LOCUS       DATNBV010000001         2303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1918561,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000001 DATNBV010000000
VERSION     DATNBV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918561"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VN627_00005"
     CDS             complement(<1..155)
                     /locus_tag="VN627_00005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HXD01667.1"
                     /translation="MIQAFAFYLFAVLCIAAGSLTILARNPVHSVLWLIVAFFNAAGL
                     MVLAGAE"
     gene            complement(168..653)
                     /gene="nuoI"
                     /locus_tag="VN627_00010"
     CDS             complement(168..653)
                     /gene="nuoI"
                     /locus_tag="VN627_00010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HXD01668.1"
                     /translation="MTAAQLIKSFTLWEFVKAHWLTLKYFFKPKATINYPYEKNPLSP
                     RFRGEHALRRYPSGEERCIACKLCEAVCPAQAITIEAEPRDDGSRRTTRYDIDMTKCI
                     YCGFCQEACPVDAIVEGPNFEFAAETREELLYDKAKLLANGDKWERAIAANLEADAAY
                     R"
     gene            complement(650..1696)
                     /gene="nuoH"
                     /locus_tag="VN627_00015"
     CDS             complement(650..1696)
                     /gene="nuoH"
                     /locus_tag="VN627_00015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HXD01669.1"
                     /translation="MTSFFQYLGLAHDWAWGISTVIGILLISLPLMLGVAMMIYVDRK
                     VWAAMALRRGPNVVGPFGLLQSFADGAKVFLQETIIPSAANKGLFLLAPIITFTVAQL
                     AWAVIPFNSGAILANINVGLLYVLAVSSLGVYGTVIAGWSSNSKYPFFSAMRASAQMI
                     SYEVSIGFILICVVLWAGTFNMNGIVLAQKGHGLGIVNGFWFNPLLFPMWVMFLISGM
                     AETGRAPFDLTEAESELVAGYQTEYSSMSFALYWLGEYANVLLMCALNATLFFGGYLP
                     PLDWAPLYLIPGVVWLLAKILFFFFVFSWVKATVPRYRYDQLMRLGWKVFLPVSLLWV
                     FLVSGYLMLTGHFA"
     gene            complement(1693..>2303)
                     /locus_tag="VN627_00020"
     CDS             complement(1693..>2303)
                     /locus_tag="VN627_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXD01670.1"
                     /translation="HMAASRMGGLMLGYARKGGIADIVGAKPKLLLSLGADEVDYANF
                     ADSIIVYIGHHGDKGAHAADIILPAAAFTEKAGTYVNTEGRVQFAEKAVFAPGDARED
                     WTILRALADALKVSVGFDSFEELRAAMAAAVPALGVEGLADYGKLPKAPKGGAKGAGA
                     EGAIAYPIKDFYLTNPIARASETMQRCSAELLHGQDFAEAAE"
BASE COUNT          445 a          698 c          796 g          364 t
ORIGIN      
        1 aactcggcgc cagcaaggac catcaggccc gccgcgttga agaaagcgac gatcagccac
       61 aggaccgaat ggaccgggtt gcgggcgagg atcgtgagcg atccggcggc gatgcacagc
      121 accgcgaaga gatagaaggc gaaagcctgg atcatggtcg cgcgccccta ccgatacgcc
      181 gcgtcggctt caaggttcgc ggcgatggcg cgctcccact tgtccccgtt cgcaagcagc
      241 ttggccttgt cgtagagcag ttcctcgcgg gtttccgcgg cgaactcgaa gttcggccct
      301 tcgacgatgg catcgaccgg gcaggcttcc tggcagaagc cgcagtagat gcacttggtc
      361 atgtcgatat cgtagcgcgt ggtgcggcgc gaaccatcgt cccgcggctc ggcctcgatc
      421 gtgatcgcct gggccgggca gaccgcctcg cacagcttgc acgcgatgca gcgctcctcg
      481 ccgctgggat agcggcgcag ggcatgctcg ccgcggaagc gcggggaaag cgggttcttc
      541 tcgtaggggt agttgatcgt cgccttgggc ttgaagaaat acttcaaggt cagccagtgc
      601 gccttcacga actcccacag cgtgaagctc ttgatgagct gggcagcggt catgcgaagt
      661 gtccggtaag catgaggtag ccgctgacga ggaacaccca cagcagcgag acgggcagga
      721 acaccttcca gcccagccgc atcagctggt cgtagcgata ccgcggcacg gtcgccttca
      781 cccagctgaa cacgaagaag aagaacagga tcttggcgag cagccagacc acgcccggga
      841 tgagatagag cggcgcccag tccagcggcg gcaagtagcc gccgaagaac agggtggcgt
      901 tgagcgcgca catcaagagg acgttggcgt attcgccgag ccagtagagc gcgaagctca
      961 tcgaggaata ttcggtctgg tagccggcga cgagctcgct ctcggcctcg gtcaggtcga
     1021 acggggcgcg gccggtttca gccatgcccg atatcaggaa catcacccac atcgggaaca
     1081 gcagcgggtt gaaccagaag ccgttgacga tgccgaggcc atggcccttc tgcgccagca
     1141 cgatgccgtt catgttgaac gtgccggccc acagcaccac gcagatcagg atgaaaccga
     1201 tcgagacttc ataggagatc atctgggcgg aggcgcgcat ggccgagaag aacgggtact
     1261 tcgagttcga cgaccagcca gcgatgacgg tgccgtagac gccgagcgag gagaccgcga
     1321 ggacgtacag gaggccgacg ttgatgttgg cgaggattgc gcccgagttg aacgggatca
     1381 ccgcccaggc gagctgggcg accgtgaagg tgatgatcgg cgcgagcagg aacaggccct
     1441 tgttcgcggc cgaggggatg atcgtttctt gcaggaagac cttggcgcca tcggcgaagg
     1501 actggagcag gccgaagggg ccgaccacgt tggggccgcg gcgcagggcc atggccgccc
     1561 agaccttgcg gtcgacatag atcatcatgg cgacgccgag catcagcggc agcgagatca
     1621 gcaggatgcc gatgacggtg gagatgcccc aggcccagtc atgggccagg ccgaggtatt
     1681 ggaagaaact ggtcattccg ccgcctccgc gaaatcctgc ccatggagca gttctgccga
     1741 gcagcgctgc atcgtttccg aggcgcgcgc gatcgggttg gtgaggtaga agtccttgat
     1801 cggataggcg atcgcgcctt ccgccccggc ccccttggca ccgcccttcg gtgccttggg
     1861 gagcttgccg tagtcagcca ggccctcgac accgagcgcc gggacggccg cggccatggc
     1921 ggcgcgcagt tcctcgaagg agtcgaaccc gaccgagacc ttcagcgcat cggcgagggc
     1981 gcgcaggatc gtccagtcct cgcgcgcgtc accgggcgcg aagacggcct tttcggcgaa
     2041 ctgcacgcgg ccttcggtgt tgacgtaggt tccggccttc tcggtgaagg cggcggcggg
     2101 caggatgatg tcggccgcgt gggcgccctt gtcaccgtgg tggccgatgt agacgatgat
     2161 ggaatcggcg aagttcgcgt agtccacctc gtccgcgccg agcgagagca gcagcttggg
     2221 cttggcgccg acgatgtcgg caattccgcc ctttcgggca tagccgagca tcagcccgcc
     2281 catgcggctg gcggccatgt gga
//
LOCUS       DATNBV010000002         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2061,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000002 DATNBV010000000
VERSION     DATNBV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2061"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VN627_00025"
     CDS             <1..556
                     /locus_tag="VN627_00025"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HXD01671.1"
                     /translation="QKTMSGQKISGKANFFIGAADAPLDPPAGWKPDRLKAKIDAGAQ
                     FVQTQFCMDPAVARRYAQRLAENGLEGFRLVIGLVPLRSARSARWIRDNLPGSIVPDA
                     IVARMEGAGDPVREGRKICDELVEQLSETPGIGGVHIMAPGNDAGLAEAVASAARIAQ
                     RQARKPAGDQAGEEASEGLLLHLQ"
     gene            783..1088
                     /locus_tag="VN627_00030"
     CDS             783..1088
                     /locus_tag="VN627_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HXD01672.1"
                     /translation="MTARIYKPTQTAMQSGLARTKEWVLEYDPEAPREIDPLMGWTSS
                     GDMRSQLKLDFATKDEAVAYAERNGIAYRLEEPKPRAAVRKAYADNFRFGRKEPWTH"
     gene            1562..1638
                     /locus_tag="VN627_00035"
     tRNA            1562..1638
                     /locus_tag="VN627_00035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1596..1598,aa:Arg,seq:tct)
     gene            complement(1897..2112)
                     /locus_tag="VN627_00040"
     CDS             complement(1897..2112)
                     /locus_tag="VN627_00040"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXD01673.1"
                     /translation="MRYTVVLGDEAEGGYSVSVPALPGCHSQGDTRDEALANIREAIE
                     LYVETLREAGEPVPTEAGKEFVEVEAA"
BASE COUNT          402 a          735 c          769 g          411 t
ORIGIN      
        1 ccaaaagacg atgtccgggc agaaaatctc cggcaaggcg aatttcttca tcggcgccgc
       61 ggatgcgccg ctcgatccac cggccggctg gaagcccgac cgcctcaagg ccaagatcga
      121 tgcgggggcc cagttcgttc agacgcagtt ctgcatggac ccggccgtgg cgcggcgcta
      181 cgcgcagcgg ctggccgaga acggcctcga aggctttcgc ctcgtgatcg gccttgtgcc
      241 tctgcggtcc gcgcgctcgg cgcgctggat tcgcgacaac ctgcccggct cgatcgttcc
      301 cgacgcaatc gttgcgcgga tggagggcgc cggcgatccc gtgcgcgagg gtcgcaagat
      361 ctgcgacgag ttggtggagc agctaagcga gacgcctggc atcgggggcg tgcacatcat
      421 ggcgccgggc aacgacgcgg gcctcgccga ggccgtcgcc agcgcggcgc ggatcgccca
      481 gcgccaggcg cgaaagcccg ccggcgacca ggccggcgag gaagcctcgg aaggcctgct
      541 gctgcatctg cagtgacacc ggcgaaatgc gtagctggat ggcccgggaa cggggcgcga
      601 acggtcgaaa tgccaccttg atttcaaagg tagttcatgt gctttgggag caggcggccg
      661 ggggttcgaa tccctctgcc ccgaccaacg ccacgcgccg tgcccggcag cggcggaacg
      721 tgtgagaacc gagcgcaggc gcccaccggc acggccgggg gcagggtgtt gtgagaaacc
      781 gaatgacggc gcgcatctac aagccgacgc agaccgcgat gcaatcgggc ctggcccgca
      841 ccaaggaatg ggtgctggag tacgatcccg aggcgccacg ggagatcgac cccctgatgg
      901 gctggaccag ctcgggcgac atgcggagcc agttgaagct cgacttcgcc accaaggatg
      961 aggctgttgc ctacgccgag cggaatggca tcgcctaccg cctggaggag cccaagcctc
     1021 gagccgcggt ccgcaaggcc tacgccgaca acttccgctt cggccgcaag gagccgtgga
     1081 cgcattgatc gtccccggcg tggccggcag acaatcctat gcaggatgac gtgcaaatat
     1141 tgtggctgtt ccatcacaga atagacgtca tccagcatga gtttgtgata agatcttgtc
     1201 tgaccgatgt aaatgtgacg gaatgttgac cgggaagacg gtggtcacgc accccgcagt
     1261 gcccgccgtt ttggggggcc ggcctaaccc tggcctgggt tcgcggtcgg ccctcatccc
     1321 attctcgccg atatgactgc gcctttccgt ggctgccgct cctggaccgt tggcgatctg
     1381 gcgccccgca gaagcttggc ggatgtcctt cccgcgtctc actggcgcgt gctaaggtct
     1441 cgtcctggcg gccgggtcag ccgcgttggg agtatctcgc agagggcctg tggcaggccg
     1501 tagcgggttg ccggtggtgt caagttcggt ctggttgggc cggcgccgtc cgggtttcca
     1561 cgtccccgta gctcagccgg atagagcatc agccttctaa gttgagggtc gcaggttcga
     1621 atcctgccgg ggacgccatc atccgatcat ctcggccatg tgatcgctgc catcacttca
     1681 acaagccgat gaactcgtcc acgtcaggcc tgcatcggcg atgatgcccc tgagcgtgcc
     1741 ggggcttgac tgtgcggtga tcaggcacga cgacccacac gctgccgctg tggaggatca
     1801 tgtgactgcc tttctgccgg acgaagacga acccggcacg ctccagtgcg gcactcaccc
     1861 tgcggccgga caagccggtc gggagcttgg ccaccgtcag gcggcctcca cctcgacgaa
     1921 ctccttgccc gcctcggtgg ggaccggctc cccggcctca cgaagcgtct caacgtagag
     1981 ctcgatcgcc tcgcggatgt tggccagggc ttcgtcgcgt gtgtcgccct ggctgtgaca
     2041 accggggaga gcgggcacac tcaccgaata gccgccttcg gcttcgtccc ccaggacaac
     2101 ggtgtagcgc atgaacaaag cccttcgtta aggaaccggt atcggtcggt tgcttggaat
     2161 gtagggcgat atggtggggg gcgccaactc cgatcaccgc cacctacctc aattcggttg
     2221 gagatcgctg ggaatccatg ctgcgattac aatactatag gtattctctg ttgtgctcgc
     2281 atctttatcg atgctcgcac gccaattgcg ttggggg
//
LOCUS       DATNBV010000003         3532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1601279,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000003 DATNBV010000000
VERSION     DATNBV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601279"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VN627_00045"
     CDS             complement(<1..933)
                     /locus_tag="VN627_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXD01674.1"
                     /translation="MTDTATKTRQLTMGEAINRALGDAMAEDETVLLFGEDVGDGEGG
                     GVVGISRGLSTKYGADRVRSTPIAEQAIIGAAVGAALGGYKPVAEIMLMNFATVAMDM
                     IVNHAAKLRFMTGGQTGVPLTVRMMTGAGISSGGQHGDYYEAWFAHVAGLKVVAPSTP
                     EDAYGLMRSCIEDPDPCIFIENLLLLAMAKGQLPDSSHRVPIGKGVVRQDGTDVTIVA
                     HSRMVVEALGAAATLAGEGISAQVIDLRSIQPWDRELVLSSAAKTGRLMVVHEATKQF
                     GIGAEIAAVASHELFGKLKAPVYRIGAAFCAVPFS"
     gene            complement(930..1934)
                     /locus_tag="VN627_00050"
     CDS             complement(930..1934)
                     /locus_tag="VN627_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HXD01675.1"
                     /translation="MKNSYVPDAATLVDIYRRAALLKANDERTRKLFTTGRLVSPYYS
                     YRGQEIIPSAISALLNDDDYVCTIYRGIHDMLAKGMPLKTLWAEVAGRVDGSCKGKGG
                     PMHLTSLEKGVMVTTGIVGSSMPIANGLAWTAKLAGNGRVAVANFGDGASNIGAFHEA
                     INLAAVWKLPVIFVCQNNEYGEHTAYEKTTSAKQIADRAVGYSIPGVRVDGNDPLAMY
                     AAAGEALERARAGEGPTLLECMTFRFHGHVFGDDDAYMPKEQKQAAIAADPVPRFRAK
                     LIADGVADEATLAAMEAAIEAEIDEAVEFALASPLPDVSELKQDVYADASVIAEDSYA
                     "
     gene            2130..2762
                     /locus_tag="VN627_00055"
     CDS             2130..2762
                     /locus_tag="VN627_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXD01676.1"
                     /translation="MASKPAERRSRQNGRKTRQRLLAAAIALWAERGSLGVTVSAVAE
                     RAGTTRRTVYHHFSTHEALITAATEYLDSELASLASGDHGPYTNPYGIVPGLAAENPE
                     LVRSVLSRMLIGDPMDNPIFASGVHFWAADLSAQGQERSPPEHITAVAIGMWYAANLV
                     ISTKTRPIERRREAERFAGTYKRIMEATLLPHAGEDQEPPMEARTNSAAK"
     gene            2794..>3532
                     /locus_tag="VN627_00060"
     CDS             2794..>3532
                     /locus_tag="VN627_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD01677.1"
                     /translation="MNQAIAVVTGATRGLGRGMARALASKGMVVHLTGRDESALAEAV
                     AEIEAAGGRANAVRCDHANDAEVAAAFARIGDAAGRIDLLVNNAAAVYPTELSGTAPF
                     WEKDVKLAEMIDVGLRSSYIATYHAAPLLLAAPAAVVAQISFYGAVSYFHGPAYGAAK
                     GGTDKMAFDMGIDFAPTPVTCISFWPGYILSDALKAVPPEYLPDDLRGALPLFETPEF
                     SGLVLEALWRDPEHKTFNGQTLIGAQLG"
BASE COUNT          653 a         1145 c         1112 g          622 t
ORIGIN      
        1 tgagaacggc accgcgcaaa aggccgcgcc gatacgatag accggcgcct tgagcttgcc
       61 gaacagctca tggctggcaa cggccgcaat ttccgcgccg atgccgaact gcttggtcgc
      121 ctcgtggaca accatgagac gcccggtctt cgctgccgat gacaggacga gttcgcggtc
      181 ccacggctgg atcgagcgaa gatcgatcac ctgcgccgag atgccttcac cggccaaagt
      241 ggcagccgca ccgagcgcct cgaccaccat gcgcgaatgg gcgacgatgg ttacgtcagt
      301 gccatcctga cgcacaacgc ccttgccgat cggcacgcga tggctgctat ccggaagctg
      361 acccttggcc atggcgagca gcaacaggtt ctcgatgaat atgcacggat cgggatcttc
      421 gatgcagctg cgcatcaatc cgtacgcgtc ctcgggcgtc gagggcgcga ccaccttgag
      481 ccccgccacg tgggcgaacc aggcctcgta atagtcgcca tgttgcccgc cggacgaaat
      541 gcccgcgccg gtcatcatcc gcacggtcaa tggcacgccg gtttggccgc cggtcatgaa
      601 acgcagcttg gctgcgtggt tcacgatcat gtccatcgcc acggtggcga agttcatgag
      661 catgatttcg gcgacgggct tgtacccgcc gagcgcggca ccgaccgcgg caccgatgat
      721 ggcctgctcg gcaatcggcg tggatcgaac ccggtcagcc ccgtacttgg tcgacaggcc
      781 gcggctgatg ccgacaacac caccgccctc cccgtcgccg acgtcttcgc cgaacaggag
      841 cacggtctca tcctcggcca tggcatcgcc gagtgcgcga ttgatcgcct cgcccatggt
      901 gagctggcgg gtcttggtag ctgtatcggt catgcgtagc tgtcctcggc aatcacgctg
      961 gcgtcagcgt agacgtcctg cttcaattca gagacatcgg ggagtggcga tgcaagggcg
     1021 aattccaccg cctcgtcgat ctccgcttcg atcgcggctt ccattgcggc aagcgtcgct
     1081 tcgtcggcaa caccgtcggc gatcagcttg gcccggaacc ggggaaccgg atcggcagcg
     1141 atggcggcct gcttctgctc cttgggcatg taggcatcgt catcgccgaa gacgtggcca
     1201 tggaagcgga aggtcatgca ttccagcagg gtcggcccct ctccggcccg agcgcgctca
     1261 agtgcctcgc cggcggcggc atacatcgcc agcggatcgt tgccgtcaac ccgcacgccc
     1321 ggaatggaat atcccacggc gcggtcggcg atctgcttgg ccgaggtcgt cttttcgtaa
     1381 gcggtgtgct cgccatactc gttgttctgg catacgaaga tcaccggcag cttccacacc
     1441 gcagccaggt tgatagcctc gtggaaggca ccgatgttcg atgcgccgtc gccgaaattg
     1501 gctaccgcca cgcgcccgtt gccggccagc ttggcagtcc aggcgaggcc attggcaatc
     1561 ggcatcgacg aaccgacgat gccggtcgtc accatgaccc ctttttcaag gctggtgagg
     1621 tgcatcgggc cgcccttgcc cttgcagctc ccgtcgaccc gcccggcgac ttcggcccag
     1681 agggtcttca gcggcatgcc tttggcaagc atgtcatgaa tgccacgata gatcgtgcag
     1741 acatagtcgt cgtcgttgag caaggcagaa atcgccgacg ggataatctc ctgcccgcga
     1801 tagctgtaat atggcgagac gagccgtccg gtcgtgaaca acttgcgggt gcgctcgtca
     1861 ttggccttga gcagcgccgc ccgacggtag atgtcgacca gcgtcgccgc gtctggaaca
     1921 taactgttct tcaccggatt ctcccaaatg cacgggcgca ccaccctgtc gggcagcccg
     1981 ttcgccgcct ctcgtaagaa caggccgctg ccgagtcaaa ggggctgatg taacagggct
     2041 gataaaacaa gctggtgaac gattgcccgt ctcgttaaga ccagatcacg ccggcacgat
     2101 cagccgtgcg cggttgggaa cgggaattga tggccagtaa gccggcagaa cgacggtcgc
     2161 ggcagaacgg ccgcaaaact cgccagcggc tcctggctgc tgcgatcgcc ctctgggcgg
     2221 agcgcggttc gctcggcgtg acggtcagcg cggtagcaga gcgcgcgggg accacccgcc
     2281 gcacggtcta ccaccacttc tcgactcacg aggctctgat cacggccgcg acggagtacc
     2341 tcgattccga gctggcgagc ctggcaagtg gggaccatgg gccctacacc aatccgtatg
     2401 gcatcgttcc cgggctggcg gcggaaaatc ctgaactggt gcgatcggtg ctcagccgca
     2461 tgctgatcgg cgacccgatg gacaacccga tatttgccag cggcgttcat ttctgggctg
     2521 ccgacctctc cgcgcaaggg caggaacgct ccccgcccga acacataacc gcggtggcga
     2581 tcggaatgtg gtatgcggcc aacctggtca tctcgaccaa gaccaggcca atcgaacgac
     2641 gccgcgaggc cgagcggttt gccggcactt acaagcggat catggaggcg accctgctgc
     2701 cgcacgccgg tgaggatcag gaaccgccaa tggaggcccg gacgaactcg gccgctaagt
     2761 aggcctggtc cgcaatccag ggaggagtag gcgatgaacc aagccatagc agtagttacg
     2821 ggggcaaccc gcggcctcgg tcgaggcatg gcccgcgcgc tcgcgagcaa ggggatggtg
     2881 gtccatctta ccggtcggga cgagagcgcc ctggccgaag ctgttgccga gatagaagcg
     2941 gcagggggac gggccaacgc cgttcgctgc gatcacgcca acgacgccga ggttgctgcg
     3001 gcctttgccc ggatcggcga tgctgctggt cgaatcgacc tgctggtcaa caatgcggcg
     3061 gcagtctacc cgaccgagct gagcggtacg gctcctttct gggagaagga cgtcaagctg
     3121 gccgagatga tcgatgtcgg cctgcgctca agctacatcg ccacttacca tgcggctccg
     3181 ctgctcctgg cggcgccggc ggcggtggtc gcccagatct cattctatgg ggcagtcagt
     3241 tacttccatg gcccggccta tggcgcggcc aagggcggga ctgacaagat ggcctttgac
     3301 atgggcatcg acttcgcacc aacccccgtg acctgcattt cattctggcc gggctacatc
     3361 ctgtccgatg cgcttaaagc ggttccgccc gaatatctgc cagacgacct gcgcggagca
     3421 cttcctctat ttgaaacgcc cgaattctcc ggcctggtcc tggaagccct gtggcgcgac
     3481 ccggagcaca agaccttcaa cggccagacg ctgatcggcg cgcagcttgg cc
//
LOCUS       DATNBV010000004         2614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2560571,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000004 DATNBV010000000
VERSION     DATNBV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2560571"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VN627_00065"
     CDS             <1..1171
                     /locus_tag="VN627_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXD01678.1"
                     /translation="VVLALDGLEVDAQMRCAAAFLNTLFDAPREEWYPALVVVDEAQL
                     FAPAAAGDVSEDARRVSLAAMTNLMCRGRKRGLAGIIATQRLAKLAKNVAAEASNFLM
                     GRTFLDIDMARAADLLGMERRQAEQIRDLARGHFLGLGPAISRRPIAVKIGEVRTRAK
                     TTIAGLMPLPTASSEDMASLLRDEPMAAPPPLPVEPEAPPMASDELLDQIEAQVPSAE
                     AVEPEPQGALFAAEETEARIAAILAEMAAEPDCTFQGAPSLFNDFTLRCRMARIPGHG
                     MDVAQFRRRFALAVAGCDAREQGWRQILAIAGDVADDLLAPFLVIARAAQMGEACPDD
                     AELARIYGTSSAGRVRRLLEHFEKAALIVVRTDYGGRRSIGIPALGVSTAPLEQA"
     gene            complement(1181..2338)
                     /locus_tag="VN627_00070"
     CDS             complement(1181..2338)
                     /locus_tag="VN627_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXD01679.1"
                     /translation="MDLNPIYRAMGTTVFEEMSALCRDGSRINLGQGFPDDNGPAALR
                     EAAARALLERSNQYPPSAGLMELREALAAFYGRTQGLALAPESFTVTSGATEALAAAI
                     LAVVSPGDEVLLFAPCYDMYAPFVRRAGGVPVIVPLHPPRWRYEHEAIAARISGRTRA
                     LILNDPLNPTGSVASPAELAMLAQLCADHDLVAICDEVWEAVRFDGQAHRSLLDFPGM
                     DSRAIKIGSAGKLFGLTGWKLGWLAAGTDLARVVARAHQFLTFTTIPATQWAVAEGLA
                     REDILAERLAGWGETRQILNSLLTEAGFAILPGPATWFTCVDLAGSGVSLADAEFSRR
                     AVHEGGVATIPISAFLEDGQTSSIVRLCHCKPPQVLAEGVRRLAVFRDGLA"
     gene            complement(2378..>2614)
                     /locus_tag="VN627_00075"
     CDS             complement(2378..>2614)
                     /locus_tag="VN627_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HXD01680.1"
                     /translation="QTYYGEHGRSRRLMGLDPENDQAEARGIVIHAADYVSPAMAANQ
                     GRVGRSQGCFAVSRGEIGSVLEMLGEGSLLYAVK"
BASE COUNT          397 a          994 c          855 g          368 t
ORIGIN      
        1 ggtcgtcctc gcgctcgacg ggctggaggt cgatgcccag atgcgctgcg ccgcggcctt
       61 cctcaacacc ctgttcgatg ccccgcgcga ggaatggtac ccggccctgg tcgtggtcga
      121 cgaggcccag ctcttcgccc ccgccgccgc cggcgacgtc agcgaggacg cgcgccgcgt
      181 cagccttgcc gccatgacca acctcatgtg ccgcggccgc aagcgcggcc ttgccgggat
      241 catcgctacc cagcgcctcg ccaagctggc caagaacgtg gctgccgaag cctccaactt
      301 cctgatgggc cgcactttcc tcgatatcga catggcccgc gccgccgacc tgctcggcat
      361 ggagcgccgc caggccgaac agatccgcga cctcgcccgt ggccacttcc tcggcctcgg
      421 tccggcgatc agccgtcggc cgatcgcggt gaagatcggc gaggtccgca cccgcgccaa
      481 gaccaccatc gccggcctga tgccgctgcc cacggccagc tcggaagaca tggcctcgct
      541 cctgcgcgac gagccgatgg ccgcgccgcc gccgctgccg gtcgaacccg aagccccgcc
      601 catggcgagc gacgagctgc tcgaccagat cgaggcgcag gtgccgtccg ccgaggcggt
      661 cgagcccgag ccgcagggcg cgctcttcgc cgccgaagaa accgaggccc ggatcgccgc
      721 catcctcgcc gaaatggccg ccgagccgga ctgcaccttc cagggcgcgc ccagcctgtt
      781 caacgacttc accctgcgct gccggatggc gcgcatcccc ggccacggca tggacgtcgc
      841 ccagttccgc cgccgcttcg ccctggcggt cgccggctgc gatgcgcggg agcagggctg
      901 gcgtcagatc ctcgcgatcg ccggcgacgt ggccgatgat ctgctcgccc cgttcctggt
      961 catcgcccgc gccgcgcaga tgggcgaagc ctgccccgac gatgccgagc tggcccggat
     1021 ctacggcacc agctccgccg gccgggttcg ccgcctgctg gagcatttcg agaaggcggc
     1081 cctgatcgtg gtccgcaccg actatggcgg gcgccgctcg atcggcattc cggcgctggg
     1141 ggtgagcacg gcgccgctgg agcaagcctg acgacgccgg tcaggccagt ccgtcccgaa
     1201 acaccgccag ccgccgcacc ccctcggcca gcacttgcgg cggcttgcaa tggcacagcc
     1261 gcacgatcga actggtctgc ccgtcctcta gaaaagccga gatcgggatg gtcgccacgc
     1321 cgccctcatg aaccgcgcgg cgcgagaact ccgcgtcggc cagtgagaca cccgagccgg
     1381 cgagatccac gcaagtgaac caggtcgccg gccccggcag gatcgcgaag ccggcctccg
     1441 tcagcagact gttcaagatc tgccgcgtct cgccccatcc ggccagccgc tcggccagga
     1501 tatcctcgcg cgccagccct tcggcaaccg cccactgggt tgccgggatc gtggtgaagg
     1561 tgaggaactg gtgcgcccgc gccaccaccc gggcgaggtc cgtcccggcc gccagccagc
     1621 ccagcttcca tccggtgagg ccgaacagct tgcccgctga gccgatcttg atcgcgcgcg
     1681 agtccatccc gggaaagtcc agcagcgacc ggtgcgcctg cccgtcgaag cgcacggcct
     1741 cccacacctc gtcgcagatc gccacgagat catggtccgc gcacagctgc gccagcatgg
     1801 ccagttcggc agggctcgcc acggagccgg tggggttgag cggatcgttg aggatcagcg
     1861 cccgcgtccg cccactgatc cgggcggcaa tcgcttcgtg ttcatagcgc cagcggggcg
     1921 ggtgcagcgg cacgatgacc ggtacgcccc ccgcccgccg cacgaatggc gcatacatat
     1981 cgtagcacgg cgcgaacagc agcacctcgt cgcccggcga aacgacggcg agtatggccg
     2041 ccgcaagtgc ctcggtcgct cctgacgtga cagtgaagga ttctggcgcc agcgccagcc
     2101 cctgggtgcg gccatagaac gcggccaacg cctcgcgcag ttccatcagc cccgccgacg
     2161 gcggatactg gttcgaccgc tccagcagcg cccgcgccgc cgcttcgcgc agcgccgcgg
     2221 ggccattgtc gtcaggaaag ccctggccca ggtttatgcg gctgccgtcg cggcatagcg
     2281 ccgacatttc ctcgaacacg gtcgtgccca tggcgcggta gatcggattg aggtccatgc
     2341 gccgcgtggt agcggcgcgc ggcaggcagg gggagtgcta tttcaccgcg tagagcaggc
     2401 tgccttcgcc cagcatctcc agcacgctgc cgatttcgcc gcgcgatacg gcgaagcagc
     2461 cctggctgcg gccgacccgg ccctggtttg cggccatggc gggcgacaca tagtccgccg
     2521 catggatcac gatgccccgg gcctcggcct gatcgttctc cggatcgagc cccatcagcc
     2581 gccgcgagcg gccatgctcg ccgtaatagg tctg
//
LOCUS       DATNBV010000005        11718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_962173,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000005 DATNBV010000000
VERSION     DATNBV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11718
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962173"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VN627_00080"
     CDS             <1..1200
                     /locus_tag="VN627_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HXD01681.1"
                     /translation="ALPLEPRLAHMLLHAARAGAAQEAAQLALLLQERGLGGRSDDLH
                     QRFDSWARDRSPRAQTSRQLAGRWAKSVPQNPGDGLPLAAILAEGFPDLIARRRDPGG
                     EQWLTAGGRGLRLDPMSPLIRAEWLAVGEAQGEAKGARITGAISLTADEVQRWLGHRI
                     ERAPTLRWVADERRVEALLEQRIGAIVLARGPDPAPDPQAIARFLADKVRTEGLGLLP
                     LSEASRALLVRAAHAGLAALSEETLRAEVDDWLLPLLTRRLDDLPAARLHEALLARLD
                     YAGRQQLDALAPPSFRSPAGTTHAIDYADPGGPSVELRVQALFGLDRHPGYGRPPQPL
                     LLKLTDPGGKPLQTTRDLPGFWRGSWADVVKDMKGRYPKHRWPAEPWTEDPSLKTKNA
                     FERGKAR"
     gene            1315..1593
                     /locus_tag="VN627_00085"
     CDS             1315..1593
                     /locus_tag="VN627_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HXD01682.1"
                     /translation="MTARIYQRPKNAMQSGKAQTDTWLLEFVPAEAKRPDPLMGWSGS
                     GDTQQQVVLQFGSEAEAQAYAARYGIETRVQVTPPKRLKLQAYADNFR"
     gene            1817..2386
                     /gene="def"
                     /locus_tag="VN627_00090"
     CDS             1817..2386
                     /gene="def"
                     /locus_tag="VN627_00090"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXD01683.1"
                     /translation="MAIREIIETPDPRLKQISARVEKFDEDLRTLVADMFETMYDAPG
                     IGLAAIQVGVPLRVVTIDLQPEDEDAEPEVCTAHGDHEHTHQPRKLEPRVFINPEILS
                     SSEDWTIYQEGCLSVPEIYAEVERPATIRARWQDLDGTVHEEQVEGLLATCLQHELDH
                     LEGILFIDHLSRLKRNMALKKLEKLRKAA"
     gene            complement(2517..2948)
                     /locus_tag="VN627_00095"
     CDS             complement(2517..2948)
                     /locus_tag="VN627_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HXD01684.1"
                     /translation="MSIEAMISEHPEVGGDYNESLGLAVRRAMLCSAICNSCADACIA
                     EMKGGADSSGSMDMSQCIRLCLDCSDVCQTTARVAMRRTGHDRQLIRAMLAVCIEACV
                     RCGEECAGHDNAHCRRCAQMCRECAEDCRAALAGLNAEVNG"
     gene            3101..4609
                     /gene="rmuC"
                     /locus_tag="VN627_00100"
     CDS             3101..4609
                     /gene="rmuC"
                     /locus_tag="VN627_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXD01685.1"
                     /translation="MNPDILSFFTLLLGLVAGVALGWFLGNRPVADWRRRFEARDLDA
                     RDLDEKFRRAITELAGTTERAQRADDQARRADEAAQRLEQLRTEHAAQLETVRLARDQ
                     VLHEKGAIAEELAALKADADNFAKRERLLVEAQEMLKKEFENAGNRVLEKAQETFLAR
                     AQDRFTESEKANAERIAAVLAPVGQRLQRYEEQVAGLEAKRVDAFGQLFGQIEALRAG
                     QEQVRAEAQRLGNSLTNAPKARGRWGERALQNVLEQCGLTEHTDFVTEHSVTTEDGRL
                     RPDAIIRIPGRKSLIIDAKVSLNAYQEAFEANDDAVRKAALDQHARSMRNHVQTLGTK
                     SYQSQFAEAPDYVVMFVPGEHFVAAALEHDPELWDFAFRNKVLLATPTNLVAIARTVA
                     EVWRQDTIAREAVEIGKAGAELYDRLAIATEHLKRMGGGLESAVSNYNKFVSSFERNV
                     LTSGKRLRDKGIAIGKREIEDVPLIDAQPRYAEVAEPVAEPEVEALPIPETE"
     gene            complement(4733..5551)
                     /locus_tag="VN627_00105"
     CDS             complement(4733..5551)
                     /locus_tag="VN627_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXD01686.1"
                     /translation="MSQRRLITGFSNPTVKFLRSLRDKKHRKAAGKFLAEGLRLLTDA
                     RESGRLPELLVMAKGRDEHPLLAALEADVLASGGEVIETEEEILGKITGKDNPQAVAG
                     VFAEFDTSLAALDRSAAPIWLVAQAMRDPGNLGTMLRTCDAVGAGGLILLDDCADPFS
                     VEAVRASMGAVFTQAIARARWDEFIAWLRGGPGQLVAASLREAVPYRGAPYAAPCFLM
                     VGNESRGLPEDYERACDLRVTMPMRGRADSLNAAIAGAVLAYEALAALVPEASA"
     gene            complement(5556..5828)
                     /locus_tag="VN627_00110"
     CDS             complement(5556..5828)
                     /locus_tag="VN627_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HXD01687.1"
                     /translation="MPSEIVEIVNQRGLHARASAKFVNAVARLPDGLDVKVAKDGTSA
                     NGGSILGLMMLGAAKGDSIEISVEGDSADQVLPKLVGLVKDGFGED"
     gene            complement(5844..6248)
                     /locus_tag="VN627_00115"
     CDS             complement(5844..6248)
                     /locus_tag="VN627_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXD01688.1"
                     /translation="MIGLILVTHGRLAEEFVHAMQHVVGRQEAVATVCIGPNDDMERR
                     RKEIAEAIRAVDSGQGAIILTDLFGGTPSNLAISLMQAGRVEVIAGINLPMLIRLAGA
                     RKTMNVVDATVAARDAGRNYITIASEFLGQDA"
     gene            complement(6325..7254)
                     /gene="rapZ"
                     /locus_tag="VN627_00120"
     CDS             complement(6325..7254)
                     /gene="rapZ"
                     /locus_tag="VN627_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HXD01689.1"
                     /translation="MDADSPQAETPGGAPQRVLLVTGLLGAGKTTVLRVLEDLGWETI
                     DNFPVRMLDQLVSAKGNDGRVPLAIGFDSRTRGFDPHRIIDRVKALTGRAGLEVTTLF
                     LDCSGQELERRYNETRRRHPLAADMPASSGIAAERELLEPLRRWADMVIVTTDFSANQ
                     LQQVIRERFGAEQAAPLTVTLSSFGFARGMPPVADLVFDMRFLDNPHWDAELRPLTGK
                     DAAVGEYVSRDPAFAEAFDRIRDLLLLLLPRYEAQGKPYVHVAFGCTGGRHRSVYVAE
                     QMAAALRDAGFSPTLLHRNLSARAADLVEGGKT"
     gene            complement(7299..7712)
                     /locus_tag="VN627_00125"
     CDS             complement(7299..7712)
                     /locus_tag="VN627_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HXD01690.1"
                     /translation="MLHQASAVAIGEQALLIEGPPGSGKSSLALALIDRGAMLIGDDG
                     VSLAGADGRILASPAPAIAGLLEVRNVGLLQYPVAERVRVALVLRLDREAERLPDGPA
                     SVELLGCRVPLVRLWPDSPVLPLRAMAALSRFGLD"
     gene            complement(7712..9235)
                     /locus_tag="VN627_00130"
     CDS             complement(7712..9235)
                     /locus_tag="VN627_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXD01691.1"
                     /translation="MFWTRRFSLTTRILAVNLIVLGLMGASVIYLDSYRKQLIAERFK
                     LARSEALIIADALAETPRSERNPLIARIGSEQVLRLRVYDARGRRLADSFLIADEPAF
                     TFADPAREPWTMQVARGIDSAMNRLTGSEPAPDYVEPAISTADAWPEIREARETGEAA
                     VRLRSAPDRTPVITAAVPYGERGEVLLTTRNARDIVDKVRYARQTLAIIIGIALVISI
                     QLSLFLARTIVQPLQLLVRAAQRVRLGRDREVVVPRLPERGDEIGLLARAVSDMSAAL
                     RQRIDAVESFAADVAHEIKNPLASLRSAVDTLGKVDDPALRQELTRIAAHDVRRIDRL
                     VTEIADASRIDAELSRTQFEPVDVVALTEALARSRAERGADGGARLVLRHAGKRCRAL
                     GDPARLERVLVNLLDNAASFSPPGGEVELATSCGDGRVRITVSDEGPGIAPEAREKVF
                     ERFHSVRPEGEDFGSHSGLGLAIARTIVTAHDGTLTATDRLDGKPGARLVLELPEAP"
     gene            complement(9279..10040)
                     /locus_tag="VN627_00135"
     CDS             complement(9279..10040)
                     /locus_tag="VN627_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXD01692.1"
                     /translation="MAPSHDAPNPPAPSSPPQQVIALVDDDRNILTTVSIALQAEGFA
                     TRVYTDGQTALKSLLENPPDLAIFDIKMPRMDGFELLQALRARSQLPVIFLTSKDEEI
                     DEARGLALGADDYIAKPFSQRLLTARIRAILRRTELVQRPQGDAANEEDGEQVVRRGR
                     LVMDRLRHQVLWDGKAVGLTVTEFLILEALATRPGVVKSRNQLMDAAYSDDVFVDDRT
                     IDSHIKRLRRKFREVDPQFSGIETLYGAGYSFADS"
     gene            10296..>11718
                     /locus_tag="VN627_00140"
     CDS             10296..>11718
                     /locus_tag="VN627_00140"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HXD01693.1"
                     /translation="MTSALSFPLDKQGFATQATIHANLGTAPLVEHALQRGEGRLTRD
                     GALLVDTGKFTGRSVKDKFVVRDATTEESINWGKINQPMSVEHWANLKADFLAALKAQ
                     DELYVADLFGGSQPEYRVAVRVITQMAWHSLFVRTLLVRPKAAELAAFAPEYTIINLP
                     SFKADPERHGSKSDTVIAVNFTEKLILIGNTEYSGEMKKGVFGLLNFLLPAQGVMPMH
                     CSANIGPDGTSAIFFGLSGTGKTTLSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMI
                     NLSAEGEPEIYATTKMFGTILENVTMDEDTRELDFTDSSKTENTRGAYPIEFIPNTSD
                     ENLGPPPSNIIFLTADAFGVLPPIARLTPEQAMYHFLSGYTAKVAGTEIGVTEPEATF
                     STCFGAAFMPRHPSVYGNLLKERIAKGGAQCWLVNTGWSGGKATTPGIKRMPIKATRA
                     LLNAALDGSLNNAEFRKDPNFGFE"
BASE COUNT         1975 a         4200 c         3736 g         1807 t
ORIGIN      
        1 gctctcccgc tcgagccgcg cctcgcccac atgctgctcc acgccgcccg cgccggcgcg
       61 gcacaagagg cggcgcagct cgccctcctg ctccaggaac gcggcctcgg cgggcgcagc
      121 gacgatctcc accagcggtt cgacagctgg gcccgcgatc gctctccccg cgcccaaacc
      181 tcccgccagc tcgccgggcg ctgggcaaaa tctgtcccgc aaaacccggg tgacggcctc
      241 ccgctcgccg cgatcctcgc cgaaggcttc cccgatctca tcgcccgccg ccgcgatccc
      301 ggcggcgaac agtggctgac cgccgggggc aggggcctgc gcctcgatcc catgagcccg
      361 ctcatccgcg ccgaatggct ggctgtcggc gaagcccagg gcgaggccaa gggcgctcgc
      421 atcaccgggg cgatctccct cacggccgat gaagtccagc gctggctcgg ccaccgcatc
      481 gaacgcgcgc cgaccctgcg ctgggtggct gatgagcgcc gcgtcgaagc cctgctcgaa
      541 cagcgtatcg gcgcgatcgt cctggcccgc ggccccgatc ccgcgccgga tccccaagcc
      601 attgcccgct tcctggccga caaggtccgc accgaaggcc tcggcctgct ccccctgtcc
      661 gaggcctccc gcgcgctcct cgtccgcgct gcccatgccg gcctcgccgc cctctccgaa
      721 gaaaccctgc gcgccgaggt cgacgactgg ctgctccccc tgctcacccg ccggctcgac
      781 gacctgccgg cggccaggct ccacgaggcc ctgctcgccc ggctcgacta tgccgggcgg
      841 cagcagctcg acgcgctcgc cccgcccagc ttccgcagcc cggccggcac cacccacgcg
      901 atcgactatg ccgatcccgg cggcccctcg gtcgaactgc gcgtccaggc cctgttcggt
      961 ctcgaccgcc atcccggcta cggccgcccg ccgcaaccac tcctgctcaa gcttaccgac
     1021 cccggcggca agcccctcca gaccacgcgc gacttgccgg ggttctggcg cggctcctgg
     1081 gccgacgtcg tgaaggacat gaagggccgt taccccaagc accgctggcc cgccgaaccg
     1141 tggaccgagg atccaagcct caagacgaag aacgccttcg agcgaggcaa ggcccgctga
     1201 aggcgaaacg tcccctcccc ggcggcgagt gcctggtgca ggggggccgc ccccgcgcga
     1261 gccacatttg caatccccct tgattccttc cccgattcgc ccgaaagcgc gcgcatgacc
     1321 gcccgtattt accagcgccc gaagaacgcc atgcagtccg gcaaggccca gaccgatacc
     1381 tggctgctgg aattcgtccc cgccgaagcc aagcggcctg atccgctgat gggctggtcg
     1441 ggctccggcg atacgcagca gcaggttgtt ctccagttcg gcagcgaggc cgaggcccag
     1501 gcctacgccg cgcgttacgg catcgaaacc cgggtccagg tcacgccgcc caagcgcctc
     1561 aagctccagg cctacgccga caacttccgc tgagcatcat atcctcccca ctcgtgggga
     1621 gggggaccgc ccgcaaagcg ggtggtggaa ggggttctcc tccgagaagc cgcgtcagtc
     1681 cgaacgcccc cgccgtcagc ccttgggggc tgccgcctcc ccactcgcgg ggaggatcgc
     1741 cactccgatt tctcccatcg caaatcgcct tcgcgccgtc ttgcgagtcg cgcgcgctga
     1801 gcttatctcg ccccccatgg ctatccgcga aatcatcgaa acccccgatc cgcgcctcaa
     1861 gcagatctcc gcccgcgtcg aaaagttcga cgaggacctg cgcaccctcg tggctgacat
     1921 gttcgagacg atgtacgatg cccccggcat cggcctcgcc gcgatccagg tcggcgtgcc
     1981 cctgcgcgtg gtaacgatcg atctccagcc cgaggacgag gacgcggagc ccgaggtctg
     2041 caccgcccac ggcgatcacg aacacaccca ccagccgcgc aagctggagc cgcgggtgtt
     2101 catcaacccc gaaatcctgt catcgagcga agactggacg atctaccagg aaggctgcct
     2161 ctcggtcccc gagatctacg ccgaagtcga acgcccggcc acgatccgcg cccgctggca
     2221 ggaccttgat ggcacggtcc acgaggaaca ggtcgaaggc ctcctcgcca cctgcctcca
     2281 gcacgagctg gaccatctcg aaggcatcct cttcatcgac cacctttcgc gcctcaagcg
     2341 caacatggcc ctgaagaagc tggaaaaact ccgcaaagct gcctgaaccc cgccagcagc
     2401 cgtcgtcccg ggctcgaccc gggaccgctg tccgctgcga agctgtgagg cgccgtgcag
     2461 gagccgccgc gcccaaaggt ccgacaccgg tcccggagca agtccgggac gacgcctcac
     2521 ccgttgacct ccgcattcaa ccccgccagt gccgcccggc aatcctccgc acactcgcgg
     2581 cacatctgcg cgcaccgccg gcagtgcgca ttgtcatgcc cggcgcattc ctcgccgcac
     2641 cgcacgcagg cctcgatgca gaccgccagc atggcgcgga tcagttgccg gtcatgcccg
     2701 gtccgccgca tcgccacccg cgccgttgtc tggcacacgt ccgagcagtc gaggcacagc
     2761 cggatgcact ggctcatgtc catgctgcct gagctatcgg ctcctccctt catctcggca
     2821 atgcacgcgt ccgcgcagga attgcagatc gccgaacaca gcatcgcccg ccgcacggcc
     2881 agtcccaggc tctcgttgta atcgccgccc acctcgggat gctcggaaat catggcttcg
     2941 atcgacatgg cacgtccttc ctgctggtcg tgcccactaa ccggcgcggg gcaggggcgt
     3001 tcccccggca gacctgtcca caaggcggtt cggccatgcg ccttgctact gcttggcttc
     3061 ctgtataagt tcccgcttcg ttccagttga gggcatggtc gtgaaccccg atatcctgag
     3121 cttcttcacc ctgctgctcg gtctcgttgc cggcgtggcg ctcggctggt tcctcggcaa
     3181 ccgcccggtc gccgattggc gccgccggtt cgaggcacgc gacctcgacg cccgcgatct
     3241 cgatgagaag ttccgccgcg cgatcaccga actcgccggc accaccgaac gcgcccagcg
     3301 cgccgatgat caggcccgcc gggcggacga ggcggcccag cggctcgaac agctgcgcac
     3361 cgaacatgcc gcccaactcg aaaccgtccg cctcgcccgc gatcaggtgc tccacgaaaa
     3421 gggcgcgatt gccgaggaac tggccgccct taaggccgat gccgacaatt tcgccaagcg
     3481 cgagcgcctg ctggttgaag cgcaggaaat gctgaagaag gagttcgaga acgccggcaa
     3541 ccgcgtgctt gaaaaggcac aggaaacctt cctcgcccgt gctcaggatc gtttcaccga
     3601 aagcgaaaag gccaatgccg aacgtatcgc ggccgtgctt gccccagtcg gccagcgcct
     3661 ccagcgctat gaagaacagg tcgccggcct cgaagccaag cgtgtcgatg ccttcggcca
     3721 gctgttcggc cagatcgagg ctctgcgcgc tgggcaggag caagtccggg ctgaggccca
     3781 gcggctcggc aattcgctca ccaatgcccc caaggctcgc ggacggtggg gcgaacgcgc
     3841 gctgcagaac gtgctcgaac agtgcggcct caccgaacac accgatttcg tcaccgagca
     3901 ttcggtgacg accgaggatg gccgcctgcg ccccgatgcg atcatccgca tccccgggcg
     3961 caagagcctg atcatcgacg ccaaggtctc cctcaacgcc taccaggagg cgttcgaggc
     4021 caatgacgac gcggtccgca aggccgcgct cgaccagcac gcccgctcga tgcgaaacca
     4081 cgtgcagacc ctcggcacca agtcctacca gagccagttc gccgaggcgc cggactatgt
     4141 ggtgatgttc gtgccgggcg aacacttcgt cgccgccgcg ctcgaacacg atccggagct
     4201 gtgggatttc gccttccgca acaaggtcct gctggccacg ccgaccaacc tcgtcgccat
     4261 cgcccgcacc gttgccgaag tctggcggca ggataccatc gcccgcgagg cggtggagat
     4321 cggcaaggcc ggggctgagc tttatgaccg gctggccatc gccaccgagc atctcaagcg
     4381 catgggcggc gggctggaga gcgcggtctc gaactacaac aagttcgtct ccagcttcga
     4441 gcgcaatgtc ctcaccagcg gcaagcgcct gcgcgacaag ggcatcgcca tcggcaagcg
     4501 cgagatcgag gacgtgccac tgatcgatgc ccagccgcgc tatgccgaag tggcggaacc
     4561 ggtggccgag ccggaagtcg aagccctgcc gatccccgaa acggagtagc accgctgcaa
     4621 aagaaaaccc ccgccagtca ggcgggggca aaggcaattg gggaggggca atgccgaagc
     4681 atcacggccg cccaattcgc ccgcccttgc tgaccatccg gtgaactgaa ccctacgcgg
     4741 atgcctcagg gaccagcgcc gccagcgcct cataggcgag gactgcaccg gcaatcgccg
     4801 cgttaaggct gtcggcccgc ccgcgcatcg gcatggtcac ccgaagatcg caggcccgtt
     4861 cgtaatcctc cggcaacccg cgcgattcgt tgccgaccat gaggaaacac ggcgccgcat
     4921 aaggcgcccc gcgatagggc acggcctccc gcagcgatgc ggccaccagc tgccccggcc
     4981 cgccgcgcag ccaggcgatg aactcgtccc accgcgcccg ggcgatggct tgcgtgaaca
     5041 ccgcccccat gcttgcccgc accgcctcca cgctgaacgg atcggcgcag tcgtccagca
     5101 ggatcagccc gcccgcgccc accgcgtcgc aggtgcgcag catggtcccg agattgcccg
     5161 gatcgcgcat ggcctgggcc accagccaga tcggcgcggc actgcggtca agcgcggcaa
     5221 ggctcgtgtc gaactcggcg aaaaccccgg ccaccgcctg cggattgtcc ttgcccgtga
     5281 tcttgccgag gatctcctcc tcggtctcga tcacctcgcc gcctgacgcc agcacgtcgg
     5341 cttcaagcgc cgccagcagc ggatgctcgt cgcgcccttt tgccatgacc agcagttccg
     5401 gcagccgccc gctttcgcgc gcgtcggtca gcaggcgcag cccctcggcg aggaacttgc
     5461 ccgcggcctt gcggtgcttc ttgtccctga gcgaacgcag gaacttgacc gtgggattgg
     5521 aaaagcccgt gatcaggcgg cgctgcgaca cgggttcagt cttcgccgaa gccgtccttc
     5581 accagcccca ccagcttggg caggacctgg tccgcgctgt caccctcgac gctgatctcg
     5641 atcgaatcgc ccttggccgc gcccagcatc atgaggccca ggatcgagcc gccgttggcg
     5701 ctggtcccgt ccttggcgac cttgacgtcc agcccgtcgg gcaggcgcgc cacggcattg
     5761 acgaacttgg cgctggcccg cgcatgcagg ccccgctgat tgacgatttc gacgatctcg
     5821 ctcggcatcc tagcccgtcc ccctcaggcg tcctgtccca gaaattcgct cgctatggtg
     5881 atgtaattgc gcccggcatc gcgcgcggca accgtggcgt cgaccacgtt catcgtcttg
     5941 cgcgcgcccg ccaggcggat cagcatcggc aggttgatcc cggcgatgac ctcgacccgg
     6001 cccgcttgca tgagcgagat cgccaggttg gaaggcgtgc cgccgaacag gtcggtcagg
     6061 atgatcgccc cctggccgct gtccaccgcg cggatcgcct cggcgatttc cttgcggcgg
     6121 cgctccatgt cgtcgttcgg gccgatgcag acggtcgcca cggcttcctg ccggccgacc
     6181 acgtgctgca tggcgtggac gaattcctcg gcaagcctgc cgtgggtcac gaggatcagc
     6241 ccgatcatga aagaggtgcg ctccgaattg ccgcccgggg ttgctgctgc cgtgaaagcc
     6301 ggcgcccggt gttggtgacc ttgctcacgt tttgccgccc tccacgagat cggcggcgcg
     6361 tgcgctcagg ttgcggtgca gcaatgtggg ggaaaatccc gcgtcgcgca aggccgcggc
     6421 catttgctcg gcgacgtaga ccgaacggtg ccgcccgccg gtgcagccga aggcgacgtg
     6481 gacatagggt ttgccctgcg cctcatagcg cggcagcagc agcagcagca ggtcgcggat
     6541 gcggtcgaac gcttcggcaa aggcgggatc gcgcgagacg tattcgccca cggccgcatc
     6601 cttgccggtc agggggcgca gctcggcgtc ccagtgcgga ttgtcgagga agcgcatgtc
     6661 gaagacgagg tcggcaaccg gcggcatgcc gcgcgcaaag ccgaagctcg acagcgtgac
     6721 cgtgagcggc gccgcctgtt ccgcgccgaa ccgctcgcgg atcacctgct gcagctggtt
     6781 ggcggaaaag tcggtggtca cgatcaccat gtcggcccag cgccgcagcg gctcgagcag
     6841 ctcgcgctct gccgcgatgc ccgagctggc cggcatgtcg gccgccagcg ggtggcggcg
     6901 gcgcgtctcg ttgtagcgcc gctccagctc ctgtcccgag caatcgagga acagggtggt
     6961 cacctcaagc ccggctcgcc ccgtcagcgc cttcacccga tcgataatcc ggtgcgggtc
     7021 gaagccgcgg gtgcgcgaat cgaagccgat ggccaatggc acccggccat cgttgccctt
     7081 ggccgagacc agctgatcga gcatgcgcac cggaaaattg tcgatcgttt cccagccaag
     7141 gtcctccagc acgcgcagca cagtcgtctt gcccgcgccg agcaggccgg tcaccagcag
     7201 cacccgctgc ggcgcgcccc cgggggtttc ggcttgaggg gaatcggcat ccatcgaggc
     7261 tgatgtgggc tcgcgatgtg agcttgtaaa ccggctgctc agtcgagccc gaaacgggac
     7321 agggcggcca tggcgcgaag tggcaggacc gggctgtcag gccagagccg aacgagcggg
     7381 acgcggcagc ccagcagttc cacgctggcc ggcccgtccg gcagtcgctc cgcctcccgg
     7441 tccagccgca ggacgagggc cacacgcacc cgctcggcga ccgggtactg cagcaatccc
     7501 acgttgcgca cctcaagcag gccggcaatc gcgggggcgg ggctggccag aatgcggccg
     7561 tccgcgcccg ccaggctcac cccgtcatcg ccgatcagca tggctccgcg atcgatcagc
     7621 gcgagggcca ggctcgactt gccgctgccc ggtggacctt cgatcagcag cgcctgttcg
     7681 ccgatcgcca cggcacttgc ctggtgcagc atcagggagc ctccggcagt tcgagcacca
     7741 gccgcgcacc cggcttcccg tcgagccgat cggtggcggt aagggtgccg tcgtgggcag
     7801 tcacgatggt gcgcgcgatg gcaaggccaa gcccgctgtg gctgccgaag tcctcacctt
     7861 cggggcgaac ggaatgaaac cgttcgaaca ccttctcccg ggcctccggc gcgattcccg
     7921 gaccctcgtc cgataccgtg atccgcaccc gtccatcgcc gcagctggtg gccagttcaa
     7981 cctcgccgcc gggcggcgag aacgaggcgg cattgtcgag caggttgacc agcacccgtt
     8041 cgaggcgggc cgggtccccc agcgcgcggc accgcttgcc ggcatggcgc aagaccagac
     8101 gggccccgcc atcggctccc cgctccgccc ggctgcgggc cagggcctcg gtcagggcga
     8161 cgacgtctac cggctcgaac tgggtgcgcg agagttcggc gtcgatccgg ctggcgtcgg
     8221 caatttcagt caccagccgg tcgatgcggc gcacgtcgtg cgcggcgatg cgggtaagct
     8281 cctggcgcag cgcggggtcg tcgaccttac cgagcgtgtc gacggcgctg cgcaggctgg
     8341 cgagggggtt cttgatctca tgggccacgt cggcggcgaa gctttccacg gcgtcgatcc
     8401 gctggcgcaa ggccgcgctc atgtccgata ccgcgcgggc gagcagaccg atctcgtccc
     8461 cgcgctcggg cagacgcggg acaaccacct cgcggtcgcg gcccagccgc acccgctgcg
     8521 ccgcccggac cagcaattgc agcggctgga caatggtccg cgccaggaac agcgaaagct
     8581 ggatcgagat gacgagggca atgccgatta tgatcgccag ggtctggcgg gcatagcgga
     8641 ccttgtcgac gatatcgcgg gcattgcggg tggtcagcag aacctcgccc cgttcgccgt
     8701 aagggacggc ggcagtgatc accggggtgc gatcaggggc cgaacgcagc cgcaccgccg
     8761 cctcaccggt ttcgcgcgcc tcgcgaattt ccggccatgc atcggccgtg gagatcgccg
     8821 gctcgacata gtcgggcgcg ggctcggacc cggtcagccg gttcatggcg ctgtcgatgc
     8881 cgcgcgccac ctgcatggtc cacggctccc tggccggatc ggcgaaagtg aaggcgggtt
     8941 cgtcggcgat gagaaaactg tcggccagac gccgcccgcg ggcatcatag acgcgcaggc
     9001 gcaagacctg ctcgctgccg atccgcgcga tcaggggatt gcgttcgctg cgcggggttt
     9061 cggccagggc atccgcgatg atcagggctt ccgagcgcgc cagcttgaac cgttcggcga
     9121 tcagctgctt gcggtaggaa tcgaggtaga tgacgctcgc ccccatcagc cccagcacaa
     9181 tcaggttcac cgcgaggatg cgcgtggtga gcgagaaccg gcgggtccag aacagctggc
     9241 ccggtgatct cgcgatgttg cgcggcgtgc ccgcgggatc agctgtcggc aaaggaatag
     9301 cccgcgccgt atagcgtctc gatgcccgag aactgggggt cgacctcgcg gaacttgcgc
     9361 cgcagccgct tgatgtggct gtcgatggtg cggtcgtcga cgaagacgtc gtcgctgtag
     9421 gccgcgtcca tcagctggtt gcggctcttc accacgccgg ggcgggtggc cagcgcctcg
     9481 aggatgagaa attcggtgac ggtgaggccg acggccttgc cgtcccacag gacctgatga
     9541 cgcagccgat ccatcaccag ccggccgcgc cggacgacct gctcgccatc ctcctcgttt
     9601 gctgcatctc cctgcggccg ctgcacgagt tccgtgcggc gcaggatcgc gcgaatgcgg
     9661 gcggtgagca ggcgctggct gaagggcttg gcgatatagt cgtccgcgcc cagggccaac
     9721 ccgcgcgcct cgtcgatctc ctcgtccttg gaggtcagga aaatcaccgg gagctgtgat
     9781 ctggcgcgca aggcctggag cagctcgaac ccgtccattc gcggcatctt gatatcgaag
     9841 atggcaaggt ccggcgggtt ttcgagcagg ctcttcagcg cggtctgccc gtcggtatag
     9901 acccgggtgg cgaagccttc cgcctgcagg gcgatggaaa cggtggtcag gatgttgcgg
     9961 tcgtcgtcga ccagcgcaat cacctgctgg ggcggggaag aaggtgcggg cgggttcggg
    10021 gcatcgtggc tgggggccat gtgctggcaa gctctcaaca agatctggac ggcaggctta
    10081 gaccggcgcg cgcggcggcg caattgatcg agaggcccgc gggcagggca ccgctgcgtg
    10141 ttttcgacgt tgatcggcgc ggcggatttg acggtttacc gtcgctggac tatgcgcggc
    10201 gccgactctc gcatacctat gcaccgagcg gaagaacgga ttttggtcca ccctccgttc
    10261 ggcctgccgg aatttggcca accgtggaga cgaacttgac ctctgccctt tcattcccgc
    10321 tcgataaaca aggcttcgcc acgcaggcga ccattcatgc caacctcggc acggcgccgc
    10381 tggtagagca tgccctgcag cggggtgaag ggcggttgac cagggatggc gcgctgctgg
    10441 tcgataccgg caagttcacc ggccgcagcg tcaaggacaa gttcgtcgtc cgcgatgcga
    10501 ccacggaaga gtcgatcaac tggggcaaga tcaaccagcc catgtcggtt gagcactggg
    10561 ccaatctcaa ggccgatttt ctcgccgcgc tgaaggccca ggacgagctc tacgttgccg
    10621 acctgttcgg cggcagccag ccggaatatc gcgtcgccgt ccgggtgatc acccagatgg
    10681 cctggcacag cctgttcgtc cgcaccctgc tcgtccgccc gaaggcggct gagcttgctg
    10741 ccttcgcgcc ggaatacacg atcattaacc tgcccagctt caaggccgat ccggaacgcc
    10801 acggcagcaa gagcgatacg gtgatcgcgg tcaacttcac cgagaagctg atcctgatcg
    10861 gcaacaccga atattcgggc gagatgaaga agggggtgtt cggcctgctg aacttcctgc
    10921 tccccgcgca gggcgtgatg ccgatgcact gctcggccaa tatcggcccg gacggcacca
    10981 gcgccatttt cttcggcctc tcgggcaccg gcaagaccac gctctcggcc gatgcttcgc
    11041 gcacccttat cggggatgac gagcatggct ggtccgacac ggcggtcttc aacttcgaag
    11101 gcggctgcta tgccaagatg atcaacctct cggccgaagg cgagccggag atctacgcga
    11161 ccaccaagat gttcggcacg atcctcgaga acgtgaccat ggacgaggat acccgcgagc
    11221 tcgacttcac ggacagcagc aagaccgaga acacccgcgg cgcctatccg atcgagttca
    11281 tccccaacac cagcgacgag aacctcgggc cgccgccgtc gaacatcatc ttccttaccg
    11341 ccgatgcctt cggcgtactg cccccgatcg cgcggctgac ccccgagcag gcgatgtatc
    11401 acttcctctc gggctacacc gccaaggtcg ccggcaccga aatcggcgtg accgagccgg
    11461 aagccacgtt ctccacctgc ttcggcgccg ccttcatgcc gcgccatccg tcggtctatg
    11521 gcaacctgct caaggagcgg atcgccaagg gcggagccca gtgctggctg gtcaacaccg
    11581 gctggtcggg cggcaaggcc accacgccgg ggatcaagcg catgccgatc aaggccaccc
    11641 gcgccctgct caacgccgcg cttgacggca gcctcaacaa tgccgaattc cgcaaggatc
    11701 cgaacttcgg cttcgagg
//
LOCUS       DATNBV010000006         2672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1282124,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000006 DATNBV010000000
VERSION     DATNBV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282124"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1180
                     /locus_tag="VN627_00145"
     CDS             281..1180
                     /locus_tag="VN627_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXD01694.1"
                     /translation="MLYRRGMEFRQIRYVLALADELHFRRAAERAHVSQPALSQALKA
                     LEEELGVRLIDRSTRGVRLTAAGELFARRGRQLLHDAELLKAGARLAGRGERASLTIG
                     LNELAGQQPAFGSILRHFRASHPEIALELADMGEAAQVSALERGQIDAGFHYRLSGSH
                     AGLATRVLNREAYRLLLPEGHPLATRDRIRLEDLADEPLIMLRREVNADTHDGILRAF
                     AERGLEPRVVLETASDAAMLTLVAEGMGLAIVMGANRRGGWQGLRLMPVEGLSLAKEF
                     VLAWNADNPAPALAQFVACAEPG"
     gene            1425..>2672
                     /locus_tag="VN627_00150"
     CDS             1425..>2672
                     /locus_tag="VN627_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD01695.1"
                     /translation="MHSNIDAVFQQKLEWMKDFVENRIRPLEFIYDYDKDAAYDLENK
                     GLRKLIVRLQQEVKDQGLWAAHLPPHLGGQGFGAVKLTYMNEMFGTSAFGPVVFGCQG
                     PDTGNQEILAMFGTDEQKEQILKPSLAQEFFTTFAMTEPQGGSDPTNLRCVARRDGDD
                     WVITGEKWFASNANHSKYMIVMTATDPDAPAHSRATMFIVPTDAPGFEIVRNIGFWSD
                     HEGTGGHPWIRFNDVRVPDSARLGPVGQGFKVAQSRLGGGRLHHAQRTLGLLKHLIDM
                     MAERAISRTSGGQPIATKQAVQMAIAESYIEYLQFRALVLQVAQMFDDQEEHGPNGRL
                     MISAIKAAMAKVVQDVTLRAVHLHGSHGLSNIMKFGEYMVVALHEGAADGVTELHLAN
                     VTKLLLRGYKPRENSNLPSETVVL"
BASE COUNT          480 a          849 c          859 g          484 t
ORIGIN      
        1 ccagcaggag gtcggcgttg ttcttggtat tgtcgagcag gccgatggtc ttgccggcaa
       61 ggccatccag ccgcgcggca atggggttca cgtcagatcc cgggtagccc accgggtcga
      121 gcagcatatc cgcgttttgc atgagggttg aggcggaggc ggcggttgcc atggcgtttg
      181 ctccagatgt ctgattggct ccgaggtaaa tgatttttcc aatcgcatcc aatcggtatt
      241 gcgaatgcat tgattgacga gggttatcag tgttcgacaa atgctgtatc gtcgtgggat
      301 ggaattccgc cagatccgct atgtcctcgc cctcgccgac gaactgcact tccgccgcgc
      361 ggcggagcgg gcccatgtct cgcagcccgc gctgtcgcag gcgttgaagg cgctcgagga
      421 ggaactcgga gtccgcctga tcgaccgatc gacccgcggc gtgcgcctga cggcagcggg
      481 cgaactgttt gcgcggcggg ggcggcaact gcttcacgat gccgaactgc tcaaggccgg
      541 cgcccggttg gcggggcggg gggagcgggc cagcctgact atcggcctca acgaacttgc
      601 cggccagcaa cccgccttcg gcagcatcct gcgccacttc cgggccagtc accccgaaat
      661 tgccctcgaa ctcgccgaca tgggcgaagc tgcccaggtc tccgcccttg aacgcgggca
      721 gatcgacgcg gggttccact accggctgag cggaagccat gccggacttg ccacccgcgt
      781 gctcaaccgc gaggcctatc ggctgctgct gccagagggt cacccgcttg ccacacgcga
      841 ccggatccgg ctggaagacc ttgccgacga gccgctgatc atgctgcggc gcgaggtcaa
      901 cgccgatacc cacgatggca tccttcgcgc tttcgccgag cgcgggcttg aaccacgggt
      961 ggtgctggaa accgcgagcg atgcggccat gctcactctc gtggccgaag ggatgggcct
     1021 ggccatcgtc atgggcgcga accgccgtgg gggctggcag ggcttgcggc tgatgccggt
     1081 ggaaggcctc tcgctcgcca aggaattcgt cttagcctgg aacgccgaca acccggctcc
     1141 cgcgctcgct caattcgttg cttgcgccga gccggggtga ccgctttcgc aactgcagga
     1201 tcgacgcggt tgccacactt gccgtcggtt cagcgtctcc aactggcgat ttgtcgttca
     1261 tgtcctgccg aatgctactg cgcgaccccg gcttcacttg acacttgtct agacaaaaat
     1321 accatgccgc gcgatggtag aaaaccacac gttccgcgca cgggcgtgac acaaaccgtt
     1381 gttttatggg cgcggcgccg cccgcggccc ctgggagaga gcgaatgcat tccaatatcg
     1441 atgccgtgtt ccagcagaag ctggagtgga tgaaggactt cgtcgagaac aggatccgtc
     1501 cgcttgaatt catctacgac tatgacaaag acgcagccta tgaccttgaa aacaagggcc
     1561 tgcgcaagct catcgtgcgc cttcagcagg aggtgaagga ccagggtctc tgggccgccc
     1621 acctgccgcc tcaccttggc gggcagggct tcggcgcagt caagctgacc tacatgaacg
     1681 agatgttcgg cacgtcggcc ttcgggccgg tggtgttcgg ctgccaggga ccggataccg
     1741 gcaaccagga aatcctcgcc atgttcggca cggacgagca gaaggagcag atcctcaagc
     1801 cttcgctggc ccaggagttt ttcaccacct tcgcgatgac cgagccccag ggcggctcgg
     1861 atcccaccaa cctgcgctgc gtcgcccgcc gtgacggcga tgactgggtg atcaccggcg
     1921 agaagtggtt tgcctccaat gccaaccatt ccaagtacat gatcgtgatg acggcgaccg
     1981 atccggatgc gccggctcac agccgcgcca ccatgttcat cgtgcccacc gacgcgcccg
     2041 gcttcgagat cgtccgcaat atcgggttct ggtccgatca cgagggcacc ggcggccacc
     2101 cgtggatccg cttcaatgac gtgcgcgtgc cggatagtgc ccgcctcggc ccggtggggc
     2161 aagggttcaa ggtggcgcag tcgcgccttg gcggaggtcg tctccatcat gcccagcgca
     2221 cgctcggcct gctcaagcac ctcattgaca tgatggccga gcgcgcgatc agccgcacct
     2281 ccggcggtca gccgatcgcc accaagcagg cggtgcagat ggctattgcc gaaagctaca
     2341 tcgagtatct gcagttccgc gccctggtgc tgcaggttgc gcagatgttc gacgaccagg
     2401 aggagcatgg tcccaatggc cggctgatga tcagcgcgat caaggcggcc atggcgaagg
     2461 tggtccagga cgtgaccttg cgggcagtcc atcttcatgg ctcgcacggc ttgtccaaca
     2521 tcatgaagtt cggcgagtac atggtggtgg ccctgcacga gggcgcggcg gacggcgtga
     2581 ccgagttgca cctggccaac gtcaccaagc tgctgctgcg cggctacaag ccgcgggaga
     2641 acagcaacct tccgagcgag accgtggtcc tg
//
LOCUS       DATNBV010000007         5796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1603547,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000007 DATNBV010000000
VERSION     DATNBV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603547"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            611..1555
                     /locus_tag="VN627_00155"
     CDS             611..1555
                     /locus_tag="VN627_00155"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXD01696.1"
                     /translation="MRSATNLLLLILAPLAGIALLFALLLVADRYFGAPGAGRDYVLR
                     IPLPQAGGRLGLPPIEGPLDASRPLVVIDAGHGGHDPGAGAGTLREKDITLALAQALR
                     VRLLADGGVRVALTRDSDRFLLLPERAEIARELKADLFISIHADSAANPDAWGATLYT
                     LSDRGSNEEASRLAASENRADTVNGVALSGQSDAVSAILVDLSQRESAAQAAEFARLV
                     LREGTGKIDFRERSIQSAAFIVLKSADVPSVLFEAGYLSNQNDAARLASPEGRQSFAE
                     VTARAIRIHFARRLAQAATGAPAPQPAAAVPEPTGTGD"
     gene            1593..4127
                     /locus_tag="VN627_00160"
     CDS             1593..4127
                     /locus_tag="VN627_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXD01697.1"
                     /translation="MADELPQQDQHLRLRRQASGLWHQSRARFSELWRTDRRFRIAGS
                     LLGLFVGLYLLVWVTVGRNLPSADTLLTYQPPLPTMVRGVNGEVVYSYARERRVQLRF
                     VDFPKPVINAFLSAEDKSFWTHGGIDYLGFVSAVADYALKIGSGERARGGSTITQQVA
                     KNILIGNEYSVTRKLKEIILARRIEGVLTKQQILELYLNEIPLGRQAFGVQAASQAYF
                     GKDVDQLQLHQAAFLAILPKAPETYSRAKYAQKAIERRNWVLDQMAKNGWATEAEVAQ
                     AKAQPLGLIPRRVESYDPSVGYYVEEVRRRLIDKYGEKAEDGPNSVYAGGLWVRTSLD
                     PQLQKAAQDSLRAGLLRYAAGKAWHGPVAHIKLGDDDWQTQLIASNISIHYQDWRIGI
                     VTERNGGNATIGFPDGTAFPLTGLPDALAAGDIIAAAPSGGNTWAVRQVPEVSGGMVV
                     ESPHSGRVLAMSGGFDAGLGSFNRATQALRQPGSTIKPFVYATALDYGMTPATMVPDQ
                     SFCVYQGAGLGEKCFRNFDMKGSGGIHTMRWGLEQSRNLMTVHIANDTGMDRVVRTIS
                     RVGIGKYDPYLSFALGAGETTVAQMVNAYSALANHGVQYAQSMIDYVQDRSGKVIWRG
                     DNRQCTGCNMAEYDGKPMPRLPLRGRQVLDARTAYQVVHMLEGVVQRGTATRLRDLGL
                     PLFGKTGTTSGPTNVWFMGGSSDIVGGVYMGFDQPRSLGGYAQGGTFAAPIMKDFIIA
                     TRARWSHTPFAAPSDIRMVKIDRASGKRVFEGEPTDDPKSSIIWEAFKADTEPPRATR
                     QDQVAAQRDEILAAIRRLREAKANGGTGEPKEEEGADFVEQQGGVY"
     gene            4364..4738
                     /locus_tag="VN627_00165"
     CDS             4364..4738
                     /locus_tag="VN627_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXD01698.1"
                     /translation="MQLARRPDHVFECNCTLCRKTGARWGYFHPEEIAATGETRSFQR
                     RDKLEPTAEIHFCPTCGTTTHFTLTPAAAARFGNTVAGVNMALANESDLAGLELRFPD
                     GNGWSGEGPFSFVREARVLGQG"
     gene            4887..5009
                     /locus_tag="VN627_00170"
                     /pseudo
     CDS             4887..5009
                     /locus_tag="VN627_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            5068..5691
                     /locus_tag="VN627_00175"
     CDS             5068..5691
                     /locus_tag="VN627_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXD01699.1"
                     /translation="MRETCRCKGGADSLARLGLDYIDLYLIHWPTPVYDSYLNAWEKL
                     IRLRESGVVRSIGVSNFTPAHIERLIRETGVTPAVNQIEMHPHFQQAELREFHRSHGI
                     VTQAWSPFGGGASGAARILDDPILTSIAARHQRTPAQVVLRWIIEQGASVIPKATSAL
                     HLRQNMDALTFALDEQDHAAIATMDRSDGRQGSDPETMRLRNVRRID"
BASE COUNT         1014 a         2049 c         1772 g          961 t
ORIGIN      
        1 attgcccttg atctgcttgt gttcggcaga ttcaaagtcg aattcctcaa tccggttgcc
       61 cttcagcaac gccacccggg tttcttccgg gtggcgcgca tcgataagca tgcgcgtggt
      121 catcaaattg tctccgtgcg cgccaccagc ttgccgcacg gggcaagcgg gcgcgtctgt
      181 tcaggtgaac gccgcgcagc gcgagagcgc gagcggcgaa acggtggttc aggttgtgcg
      241 tgtctgccgg atcgagaagg cgcggcgatt gcgaacgcgc attgtcatcg aacccaggtc
      301 ccaccggcat cgtcgcccgc agtggcgtcg aactccggaa cggggcaggg cttctcatac
      361 cagcatcaac tcggttacgt tccgccgggc gcggatggcc cggctggtcg aagcaaggtt
      421 tctatcctct cttccgttaa atctttcagc catttgatag gcgaaaaacc gtgcgggagg
      481 attgtggcga aactgcctcg gactgcctgc ctagcattgc cctttcggcg cggcaagtta
      541 cttgcttgcg ccgggccacc cgctcaggcg attgcgggcg gcggcctgaa cgcctaacca
      601 gcatgggttc atgcgctccg ccaccaatct gttgctcctg atcctcgcgc cgctcgccgg
      661 catcgcgctg ctgttcgcgc tgttgctggt ggccgatcgc tacttcggcg cgccgggcgc
      721 ggggcgcgac tatgtgctgc gcattccgct gccgcaggcc ggggggcggc tgggcctgcc
      781 gccgatcgaa ggcccgctcg atgccagccg tccgctggtg gtgatcgacg ccggccatgg
      841 cggccacgat cccggcgccg gggcgggcac cctgcgcgag aaggacatca ccctcgcact
      901 cgcccaggcc ctgcgcgtgc gcctgctggc cgatggcggg gtgcgcgtcg cgctcacccg
      961 tgacagcgac cgcttcctgc tcctgcccga gcgcgccgag atcgcccgtg agctcaaggc
     1021 cgatctgttc atttcgatcc atgccgacag cgcggccaat cccgatgcct ggggcgccac
     1081 gctctacacc ctgtccgatc gcggctcgaa tgaggaagcc tcgcgcctcg ccgcgtcgga
     1141 aaaccgcgcc gacacggtca acggcgttgc cctctcagga cagagcgatg ccgtcagcgc
     1201 catcctcgtt gatctgtcgc agcgcgaaag cgccgcccag gccgccgagt tcgcgcgcct
     1261 cgtgctgcgc gaaggcaccg gcaagatcga ttttcgcgaa cgttcaatcc agtcagccgc
     1321 cttcatcgtc ctcaagtcgg ccgacgtgcc ctcggtgctg ttcgaggccg gctatctcag
     1381 caatcagaac gatgccgccc ggctcgcctc gcccgaaggc cggcaaagct tcgccgaagt
     1441 caccgcccgc gccatccgca tccacttcgc ccgcaggttg gcgcaagcgg caaccggcgc
     1501 acccgcgccc cagccggccg cggcggttcc cgagccgacc ggaacaggcg actagccccg
     1561 cgcccgcgca ctctctatag accctcccga acatggccga cgagcttccc cagcaagacc
     1621 agcatctgcg cctgcgccgc caggcgtcgg gcctctggca ccagtcccgc gcccgttttt
     1681 ccgagctgtg gcggaccgac cggcgctttc gaatcgccgg ttccctgctt ggcctgttcg
     1741 tgggcctcta cctgctggtc tgggtcaccg tcggccgcaa cctgcccagc gccgataccc
     1801 tgttgaccta ccagcccccg ctgccaacca tggtgcgcgg ggtgaatggc gaggtcgtct
     1861 attcctatgc ccgcgaacgc cgcgtccagc tgcgcttcgt cgattttccc aagcccgtga
     1921 tcaacgcctt cctctcggcg gaagacaagt ccttctggac tcacggcggg atcgattatc
     1981 tcggcttcgt cagtgccgtg gctgactatg cgttgaagat cggctcgggc gaacgcgctc
     2041 gcggcggctc caccatcacc cagcaggtcg ccaagaacat cctgatcggc aacgagtatt
     2101 cggtcacccg caagctcaag gaaatcattc tcgcgcggcg catcgaaggc gtgctgacca
     2161 agcagcagat cctcgaactc tacctcaacg agatcccgct cggccggcag gccttcgggg
     2221 tccaggcggc gtcgcaggcc tatttcggca aggatgtcga tcagctccag ctccatcagg
     2281 ccgctttcct cgcgatcctg cccaaggctc ccgaaaccta cagccgcgcc aagtacgcac
     2341 agaaggcgat cgagcggcgc aactgggtgc tcgatcagat ggccaagaac ggctgggcca
     2401 ccgaggcgga agtcgcccag gccaaggccc agcccctcgg cctgatcccg cgccgggtgg
     2461 agtcctacga tccgtccgtc ggctactatg tcgaggaagt ccgccgccgc ctgatcgaca
     2521 agtatggcga gaaggcggaa gacgggccca actcggtcta cgcgggcggc ctgtgggtgc
     2581 gcaccagcct cgatccgcag ctccagaagg ccgcgcagga ttcgctgcgc gccggtctcc
     2641 tgcgctatgc ggccggcaag gcctggcatg gccccgtcgc ccacatcaag ctgggcgatg
     2701 acgattggca gacccagctg atcgccagca acatctcgat ccactatcag gactggcgga
     2761 tcggcatcgt caccgagcgc aatggcggca atgccacgat cggcttcccc gatggcacgg
     2821 ccttcccgct caccggcctg cccgatgccc tggccgcggg cgatatcatc gccgccgcgc
     2881 cttcgggcgg caatacctgg gcggtgcggc aagtgcctga agtgtccggc ggcatggttg
     2941 tcgaaagccc acattcaggc cgcgtcctgg cgatgtccgg cggcttcgat gccgggctcg
     3001 gctcgttcaa ccgggccacc caggcgctgc gccagcccgg ttcgaccatc aagcccttcg
     3061 tctatgccac cgcgctcgac tacgggatga cccccgccac catggtcccc gaccagtcgt
     3121 tctgcgtcta ccagggcgcc ggccttggcg agaaatgctt ccgcaacttc gacatgaagg
     3181 gttccggcgg catccacacc atgcgctggg gcctcgaaca gtcgcgcaac ctgatgaccg
     3241 tccacatcgc caacgatacc ggcatggacc gcgtcgtgcg caccatcagc cgtgtcggca
     3301 tcggcaagta cgatccctac ctgtccttcg cgctcggcgc gggcgaaacc accgttgccc
     3361 agatggtcaa cgcctattcc gcgctcgcca atcacggcgt ccagtatgcc cagtcgatga
     3421 tcgactatgt gcaggaccgc agcggcaagg tcatctggcg gggcgacaat cgccagtgca
     3481 ccggctgcaa catggccgag tatgacggca agccgatgcc gcgcctgcct cttcgcgggc
     3541 gtcaggtgct cgatgcgcgc acggcctacc aggtcgtcca catgctcgaa ggcgtggtcc
     3601 agcgcggcac ggccacccgc ctgcgcgatc tcggcctgcc gctgttcggc aagaccggca
     3661 ccacttcggg accgaccaac gtctggttca tgggcggctc gtcggacatt gtcggcgggg
     3721 tctacatggg cttcgaccag ccgcgctcgc tcggcggcta tgcgcagggc ggcactttcg
     3781 ccgcgccgat catgaaggac ttcatcatcg ccacccgcgc gcgctggagc cacaccccct
     3841 tcgccgcgcc gagcgacatc cgcatggtca agatcgaccg cgccagtggc aagcgcgtgt
     3901 tcgagggcga gccgaccgac gatcccaagt catcgatcat ctgggaagcc ttcaaggccg
     3961 ataccgaacc gccccgcgcc acccgccaag accaggtcgc cgcccagcgc gacgaaatcc
     4021 tggccgccat ccgccgcctg cgcgaagcca aggccaacgg cggcactggc gaacccaagg
     4081 aagaagaagg cgccgacttc gtcgaacaac aaggcggcgt ctattgatcc tccttccgcg
     4141 cggcagcgga cggggacgcg caaatcctcc ccgaaccggg ggagtcgcag acggcggccc
     4201 gaagggtcgc cagcgggacc atccgcagga tggtggaggg gcacccgcgg ttcttcacaa
     4261 cgccggcttg atgggtcctc cggaaaagcc ctgtcacagc ccaccaaacc cgtgctaacc
     4321 cactgaaact atgaccctat cctgcctctg cggcgctgcc caggtgcagc tcgcccgccg
     4381 ccctgaccat gtcttcgaat gcaattgcac cttgtgccgc aagaccggcg cgcgctgggg
     4441 ctacttccat ccggaggaaa tcgccgccac cggcgaaacc cgcagcttcc agcgccgcga
     4501 caagctcgaa cccacggccg aaatccactt ctgcccgacc tgcggcacca ctacccattt
     4561 cacccttacg cctgccgctg ccgcccgctt cggcaacacc gtggccggcg tgaacatggc
     4621 tctcgccaac gagtccgacc tcgccggcct cgaactgcgc ttccccgacg gcaatggctg
     4681 gagcggggaa gggccgttct ccttcgtgcg cgaagcgcgg gtgctggggc agggctgacc
     4741 caccagcatc aggtcagagc ggtgcgatct gacaacaaac agcttggtcc tcggctagga
     4801 caccccacct cccggagaat tgttccatga cctaccagat gcaattgaac gatgggcggg
     4861 tcatgccgca acttggcctg ggcctgatgc ggatcgggtc ggaaagcgaa accgagctcg
     4921 cgctggatcg cggtgtcgcc gccggatacc ggttgttcga cacggccgcc atctacggta
     4981 acgagcgcga ggtcggcgcc gcactcgtca agcacgccgc aatccggccg gagctgttca
     5041 tcaccacgaa gctgtggaac gaccaccatg agggagacgt gccgctgcaa ggggggggca
     5101 gacagcctcg cccggctcgg gctggactac atcgacctct atctgatcca ctggcccacc
     5161 ccggtttacg acagctacct gaacgcatgg gaaaagttga tccgcctgcg cgaatccggc
     5221 gtggtccgct cgatcggggt gtcaaatttc acgcccgctc atatcgaacg cctgatccgg
     5281 gaaaccgggg tgacgccggc ggtcaaccag atcgagatgc acccgcattt ccagcaagcc
     5341 gagctgcgcg aatttcatcg ctctcacggc attgtcaccc aggcctggtc gccgttcggc
     5401 ggcggcgcaa gcggagcggc gcgcatcctc gacgatccaa tcctcacgag catcgcggca
     5461 cggcatcagc gcacgcccgc gcaagtcgtg ctgcgctgga tcatcgaaca gggagcctcg
     5521 gtgatcccca aggcaaccag cgccttgcac ctgcggcaga acatggatgc gctgactttc
     5581 gctctcgatg agcaagacca tgccgccatc gcgacgatgg accgctccga cgggcgacaa
     5641 ggttcggacc ctgagaccat gcgcctgcga aacgtccggc gtatcgacta gggtcaggac
     5701 ctgttacctg cgtcatcgag gcttgagtca aaatggctga gtgcaaggaa ggggcaggaa
     5761 tacatccgta tttcaaggct tcctgacgcg gcaatg
//
LOCUS       DATNBV010000008         3428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3200591,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000008 DATNBV010000000
VERSION     DATNBV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3200591"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VN627_00180"
     CDS             <1..780
                     /locus_tag="VN627_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXD01700.1"
                     /translation="AAAMNQVTAIVRETAQGAAKVQQAIADAHREATEGGETVERAVA
                     AMTSIEQGAREITQIINVIDGIAFQTNLLALNAGVEAARAGDAGKGFAVVANEVRALA
                     QRSADAARDIKALITGSTEQVEGGVRLVHETGELLGKIVGSVGQISTSVTEIAKATEN
                     QSVSLEQVNSAIGEMDRMTQQNAAMVEQSTAAARSLADEANSLSGQVAQFRTGSAGGR
                     PSAPVVPLKQTARPQARPLPTRGNLALRPDPVADGDDWSEF"
     gene            815..1075
                     /locus_tag="VN627_00185"
     CDS             815..1075
                     /locus_tag="VN627_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXD01701.1"
                     /translation="MNTVLLPAACTRSAAEALLPELLAAVGNGPVHIDGTAVTQVGQA
                     VLQLLVSARRSGEGATIAPSAALLDAAILAGLSGELFDEVAA"
     gene            1072..3222
                     /locus_tag="VN627_00190"
     CDS             1072..3222
                     /locus_tag="VN627_00190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HXD01702.1"
                     /translation="MSPEEIQQIFFVECEESLAAAEAGLAALREGNRDSETINAVFRA
                     IHSIKGGAGAFAFGALQAYTHTFETLLSDVRDGTVPIGEPLVDLLLRALDTLSDHVTA
                     ARESDAPPADADLLAELDAARTGSVSTPAPVAAQAEPAGEANSTEAPNDPDDELGLGL
                     DDMLNDLLGTGPAAPAPETVSWQLQVRPHGAAMDNGSEPLLCLRELARLGGECQVCDT
                     VGVPPLESFRVDEGYLAWTFVMPGTVDQSAARDIFEFNANEFDLAFDGAPLPTAGAIA
                     PEAAKAAQPAPVPAIAAAQPTKPATPPADPAGATPATAAGAGASPAGAAGQSIRIDLA
                     KLDRLIDSVGELVIAQAMLAQRLGGQDIAAGEELGLLESLTRDIQENAMSIRAQPIGS
                     VFSRVPRILRELAASTGKHVRLEVIGEGTELDKTVIERLGEPLTHLIRNAVDHGIENA
                     EERIAAGKNPEGTLTLSAEQRSGRIRIRIGDDGKGIDCERVLAKAIEKGLVAPEAQLS
                     REEIENLIFAPGFSTAQTVSNVSGRGVGMDVVRQNVKDLGGRITIETEPGKGTSFILT
                     LPLTLAISDGMIISVGDQTLVVPLAHVVESLRPDETQVQAMGADRQMLNVRGQFIPVI
                     PVGRAIGATGCCGSPDKGVLIVVDSEVAGQAALLVDDISDQRQFVIKSLDANYRTVTG
                     VAGATILGDGRVALIVDVDGLVSEALRSPERLAA"
     gene            3219..>3428
                     /locus_tag="VN627_00195"
     CDS             3219..>3428
                     /locus_tag="VN627_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01703.1"
                     /translation="MKLTEPTGLADGVPGVSPGVYNAADFQAVAEIVYGEAGIVLAQG
                     KAMLVYSRLAPLVRRAGCGTFANFVA"
BASE COUNT          599 a         1193 c         1133 g          503 t
ORIGIN      
        1 gccgcggcga tgaaccaggt gaccgccatc gtgcgcgaaa cggcgcaggg cgccgccaag
       61 gtccagcagg ctatcgccga cgctcaccgc gaggcgaccg agggcggcga aaccgtcgag
      121 cgtgccgtcg cggcgatgac ctccatcgaa cagggcgccc gcgaaatcac gcagatcatc
      181 aacgtcatcg acggaatcgc cttccagacc aacctcctgg cgctgaacgc cggggtcgag
      241 gctgcgcgcg ccggcgatgc cggcaagggc ttcgccgtgg tcgccaacga ggtgcgcgcg
      301 ctcgcccagc ggtcagccga tgccgcgcgg gacatcaagg ccctgattac cggctcgacc
      361 gaacaggtcg agggcggcgt acggctggtc catgaaaccg gcgaactgct gggcaagatc
      421 gtcggcagcg ttggccagat cagcacctcg gtgaccgaga tcgcaaaggc caccgaaaac
      481 cagtcggtca gcctggaaca ggtcaactcc gccattggcg aaatggaccg gatgacccag
      541 cagaatgcgg cgatggtcga gcagtcgacc gccgccgcgc ggtcgctggc ggatgaggcg
      601 aacagcctgt cgggccaggt cgcccaattc cgcaccggct cggccggagg caggccatcg
      661 gcccccgtcg tgccgctgaa gcaaaccgca agacctcagg ctcgccccct gccgacccgc
      721 ggcaacctcg ccttgcgccc cgacccggtg gccgatggcg atgactggtc cgaattctga
      781 agcccgctcc tgcccctcgt gtgaaaggcc gatgatgaac accgtgctcc tgcccgccgc
      841 ctgcacccgc agtgccgccg aagcactcct gcccgagctg ctcgcggccg tgggcaacgg
      901 ccccgtccac atcgacggaa cagccgtgac ccaggttggc caggctgtcc tgcagctcct
      961 cgtcagcgcc cggcgcagcg gcgaaggggc aacgatcgcg ccatcggcgg cgctgctcga
     1021 tgccgccatc cttgccgggc tatccggcga attgttcgat gaggtggcgg catgagcccg
     1081 gaggaaatcc agcagatctt cttcgttgaa tgcgaagagt ccctggccgc cgcggaggcc
     1141 ggcctagcgg ccctgcgcga aggcaatcgc gacagcgaga cgatcaacgc ggttttccgc
     1201 gcgatccatt cgatcaaggg cggcgccggg gcgttcgcct tcggcgcgct ccaggcctat
     1261 acgcatacgt tcgaaacgct gctgtcggac gtacgtgacg gcactgtgcc gatcggcgaa
     1321 ccgctcgtcg acctgctgct gcgcgcactc gacaccttga gcgatcatgt caccgccgcc
     1381 cgcgagagcg atgcgccccc ggccgatgcg gacctgctgg cggaactcga tgccgcccgc
     1441 accggctctg tgagcactcc cgcgccggtc gccgcccaag ccgaacctgc cggggaggcg
     1501 aattcgaccg aggctccgaa tgatccggac gacgagctgg gtctcgggct ggacgacatg
     1561 ctcaatgacc tgctcggcac gggcccggcg gctccggccc cagaaaccgt ctcttggcaa
     1621 ctccaggtgc gcccgcatgg cgcggccatg gacaatggca gcgaaccgct gttgtgcctg
     1681 cgcgaactcg cccggctggg cggcgaatgc caggtctgcg ataccgtagg cgtgccgccg
     1741 ctggaaagct tccgcgtcga cgagggttac cttgcctgga ccttcgtcat gcccggaacg
     1801 gtcgatcagt ccgccgcgcg cgacatcttc gaattcaacg ccaacgaatt tgacctggcg
     1861 ttcgacggtg cccccctccc gacggcaggt gccatcgctc ccgaggccgc aaaggcggca
     1921 cagccagccc cggtgcctgc cattgccgcc gcgcagccga ccaagccagc gactccgccc
     1981 gccgatccgg ccggggcaac tccggccacc gccgcgggcg caggggcgtc tccggcgggc
     2041 gcggcgggcc aatcgatccg gatcgatctg gccaagctcg atcggctgat cgattcggtg
     2101 ggcgaattgg tgattgccca ggcgatgttg gcccagcgac ttggcgggca ggacattgcc
     2161 gctggcgaag aactggggct gctggaatcg ctgacccgcg acatccagga aaacgccatg
     2221 tcgatccgcg cccagccgat cggttcggtc ttcagccgcg tgccgcgcat cctgcgcgag
     2281 ctggccgcct cgaccggcaa gcatgtccgc ctggaggtga tcggcgaggg caccgaactc
     2341 gacaagacgg tcatcgagcg attgggcgaa ccgctgaccc acctcatccg caacgcagtc
     2401 gaccacggca tcgagaacgc cgaggagcgc attgccgccg gcaagaaccc ggaaggcacg
     2461 ctgacgcttt cggccgaaca gcgctccggc cgcattcgca tccgcatcgg cgacgacggc
     2521 aagggcatcg actgcgagcg ggtcctcgcc aaggccattg aaaagggcct ggttgcgccc
     2581 gaagcccagc tttcccgaga ggagatcgag aacctgatct tcgcgcccgg cttctccacg
     2641 gcgcaaaccg tatcgaacgt atcgggccgg ggcgtgggca tggacgtggt ccgccagaac
     2701 gtgaaggacc tgggcgggcg gatcaccatc gagaccgaac cgggcaaggg gaccagcttc
     2761 atcctgaccc tgccgctgac cctcgccatt tcggacggca tgatcatttc ggtcggcgac
     2821 cagaccctgg tcgttccgct cgcccacgtg gtcgaaagcc tgcggcccga cgagacacaa
     2881 gttcaagcga tgggcgcgga ccggcagatg ctcaatgtgc gcggacaatt cattccggtt
     2941 atcccggtag gtcgggccat cggggccacc gggtgctgcg gatcgcccga caagggcgtg
     3001 ctgatcgtgg tcgacagcga agtggccggc caggccgcgc tgctggtcga cgatatttcc
     3061 gaccagcgcc aattcgtcat caagagcctc gatgccaact accgcacggt taccggagtc
     3121 gccggcgcga ccattctggg cgatggccgg gtggcgctga tcgtcgacgt cgacggtctg
     3181 gtgagcgaag cactgcgcag ccccgaaagg ctggcggcat gaagctcacc gaaccaaccg
     3241 ggctggccga cggcgttccg ggcgtcagcc ccggagtcta caacgcggcc gatttccagg
     3301 ccgtagccga gatcgtctac ggcgaggccg gcatcgtcct tgcccagggc aaggccatgc
     3361 tggtctattc acgtctcgcg ccgctggtcc gccgcgccgg ctgcgggacc ttcgccaact
     3421 tcgttgcg
//
LOCUS       DATNBV010000009         6186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_7981,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000009 DATNBV010000000
VERSION     DATNBV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7981"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..2287)
                     /gene="recG"
                     /locus_tag="VN627_00200"
     CDS             complement(224..2287)
                     /gene="recG"
                     /locus_tag="VN627_00200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXD01704.1"
                     /translation="MRPDRLNPLFAETAGLKGVGAGIMRPLDKLGLTRVRDLAYHLPD
                     RFVQRRAVTDLDEAGVGEQIVVRLTPREYRSGGSARAPFRVHAEDAAGNICALTYFGR
                     NGGWARKQLPLGEPRWIAGRLDQFGQTLQIVHPEHVAEDGGVALGTLCEAVYPLSEGL
                     TQGRIAALVAQALGHLPDLPEWIEPGLMARVGWPDWKSALLEAHKGEHKPSRDRLAYD
                     ELLANSLALMLVRNANRARAGQPLQGDGRLREKLSLPFGLTGAQRRSIAEIEGDMVQG
                     SPMLRLLQGDVGAGKTVVALEAMLVAVEAGAQAALLAPTEILARQHHATLTRMASGTG
                     VTIALLTGRDKGRARESILMGLIDGSIDICVGTHAIFQESVSYRNLALVVIDEQHRFG
                     VGQRLMLTQKGKRTPHCLAMTATPIPRTLTLAQYGEMDVSRLDEMPPGRQPIDTRVVA
                     VERMGDVIGALERHLAGGQQAYWVCPMVRESETDDIAAAEARYAELRHRLGEDTVVLV
                     HGQLKPEVKDAAMERFASGQAALLVATTVIEVGVDVPNATLMVIEQAERFGLAQLHQL
                     RGRVGRGAEKSTCLLLRGDALSEVARERLALMRETQDGFRLAEEDLRLRGGGELLGTR
                     QSGDTPFRVASLEQIAELLPLAHDDARLLIERDGGLTGERGEAARLLLYLFERDYGVQ
                     LLRGG"
     gene            2419..2820
                     /locus_tag="VN627_00205"
     CDS             2419..2820
                     /locus_tag="VN627_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HXD01705.1"
                     /translation="MLLPHGTIVALVDGHNFHVFRNAGNEATPELEALPAPALDSHNH
                     SGTGHHSRPGNHAGTLVAEDAHAIAAVNWLNDQVLAHKLEHLILIAAPRTLGEMRRHY
                     HKQLEQVLMGELAKDLSGRKGPEILAALRGK"
     gene            2916..3179
                     /locus_tag="VN627_00210"
     CDS             2916..3179
                     /locus_tag="VN627_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HXD01706.1"
                     /translation="MDEPRLKRLQFRAWHRGTREADYMIGCYFDRFHGEWGEAEIAWF
                     EALIEEEDVDIMGWALGTLAVPEEFAGPLMDRMKLLDYVEIPR"
     gene            3308..4402
                     /locus_tag="VN627_00215"
     CDS             3308..4402
                     /locus_tag="VN627_00215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXD01707.1"
                     /translation="MLRRLSVAGLGEGAVRAQWDDLAARAAEPNPFLESWFLTPALAA
                     LDPAGAAEVMVLEAGGRWLGLLPLARLRRYYGRPLPHLGNWSHGNAFLGAPLVARGAE
                     AAFWTALLAHADGAAGPALFLHLTDQPLTGPLAIALMRVCAGQRRPIGLVHREERALL
                     ASDLSPEAYLEAALTGKKRKELRRQRARLSDQGALAFARKRDEAGLAEWTRDFLALEA
                     AGWKGRAGSALAADPATTALFAAALEGSAARNRLERLTLSLDGTPVAMLATFLAPPGA
                     FAFKTAFAEDFARYSPGVLLQRENLGLLADPDLAWCDSCAAANHPMIDHLWRERRAIG
                     RFSVAIGGRARRAAFSLLLKAEMARSGSQA"
     gene            4399..5241
                     /locus_tag="VN627_00220"
     CDS             4399..5241
                     /locus_tag="VN627_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXD01708.1"
                     /translation="MTVFPPTALATFAQNYPEVPHKLVHTLGSHPLLDLEALAELAEA
                     LPAASVEYNSADLPIGIDRKPDRTGIPIGETIRRIGESGSWAALKNIEQVPAYADLLH
                     SLLAELRAAIEPRTGAMLRLEGFIFITSPGGVTPYHFDPEHNILLQVRGTKTMTQFPA
                     GDPRYAPDAVHEAYHTGGGRELRWEDGLLPGGTEFALAPGEALFVPVMAPHFVRNGPE
                     SSVSLSITWRSKWSFAEADARALNKVLRRFGLTPRAPARWPASNRAKSIGWRMLRRFG
                     LAPG"
     gene            5374..>6186
                     /locus_tag="VN627_00225"
     CDS             5374..>6186
                     /locus_tag="VN627_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXD01709.1"
                     /translation="MTATTVPDLSTRTDFIPKEDYISPEFAKGEKERMWPASWLMACR
                     EEDLAEPGQFYTFNIADDSIIINRDEAGRIQAHHNVCPHRGRRLTAGCGHATKGFYCN
                     YHGWQWNLDGSNKVMIDGEEWSGIGQADVGLRPVRVDTWGGFVFVNIDGKADPLLDWL
                     HPWPEMWESYRIQDMRYCWSKSTRLDCNWKVAIEAFLEGYHAQTTHRQVNNCDGGSHS
                     ICQTFGRHAMFSMYQVGHLGERSHNAKYLPPYNSDLFEGVTDLRERIALYMKV"
BASE COUNT         1003 a         2311 c         1901 g          971 t
ORIGIN      
        1 gacctgatcg agctcaagga ccggatgctg gcgcgcaagg aagagctgat ggccatggcc
       61 aagaacggcg gcctgccgcc agcgcaggtc cgcgtcgttc aggtctgaat gcgtccgggc
      121 cggcctgccg cctgatcgtg caggttggcc cggccaacca tcaagcgccg gagcggcccg
      181 gcgcacaggc tgcggcgcgc atgattacgc gcctgatggg ggcttacccg ccgcgcagca
      241 actgcacccc atagtcccgt tcgaacaggt agaggagcag ccgggccgct tcccctcgct
      301 caccggtaag ccccccatcg cgctcgatca ggaggcgcgc atcgtcgtgg gcgagcggca
      361 gcagttcggc gatctgttcc agcgaagcca cgcgaaacgg cgtgtcgccc gattggcgcg
      421 tgccgagcag ctcacccccg ccgcgcagcc gcaggtcttc ctccgccagc cggaacccgt
      481 cctgcgtttc gcgcatcagc gccagccgct cacgcgcgac ctcgctcagc gcatcgccgc
      541 gcagcagcag gcaggtcgac ttctccgctc cacggcccac ccgcccgcgc aactggtgca
      601 gttgggccag gccaaaccgc tcggcctgtt cgatcaccat cagcgtcgcg ttgggcacgt
      661 cgacgcccac ctcgatcacg gtcgtggcga ccagcagcgc cgcctggccg ctggcgaacc
      721 gctccatcgc ggcatccttg acctcgggct tgagctggcc gtggacgagc accaccgtat
      781 cctcgcccag gcggtggcgc agttcggcat agcgggcctc ggcggcggcg atatcgtcgg
      841 tttcgctctc gcgcaccatc gggcaaaccc agtaggcctg ctgcccgccg gcgagatgcc
      901 gctcgagcgc gccaatcacg tcgcccatgc gctcgaccgc gaccacccgt gtgtcgatcg
      961 gctggcgccc tgggggcatc tcgtccagcc ggctcacatc catctcgcca tactgggcga
     1021 gcgtcagcgt gcgcgggatc ggggtggcgg tcatcgccag gcaatggggc gtgcgctttc
     1081 ctttttgcgt gagcatcagc cgctggccga ccccgaagcg gtgctgctcg tcgatcacca
     1141 ccagcgcgag gttccggtag ctcacgcttt cctggaagat cgcatgggtg cccacacaaa
     1201 tgtcgatgga gccatcgatc aggcccatca ggatggattc gcgcgcgcgc cccttgtcgc
     1261 gccccgtaag gagcgcaatt gtcactccgg tgcccgacgc catccgggtc agcgtcgcgt
     1321 gatgctgccg ggcaaggatc tcggtgggcg ccagcaaagc ggcctgtgcc cccgcctcca
     1381 ccgccaccag catcgcctcc agcgccacca cggtcttgcc cgcgccgaca tcgccctgca
     1441 gcagccgcag catgggcgag ccctggacca tgtcgccctc gatctcggca atcgaacgcc
     1501 gctgcgcccc cgtcagcccg aacgggagcg acagcttttc ccgcagccgc ccgtcgccct
     1561 gcagcggctg ccccgcccgc gcgcggttgg cattgcgcac cagcatcagg gcaaggctgt
     1621 tggccagcag ctcgtcgtag gccaaccggt cacgcgaggg cttgtgctcg cccttgtgcg
     1681 cctccagcag ggcgcttttc cagtcgggcc agccgacccg ggccatcagc cccggctcga
     1741 tccactcggg caagtccgga aggtggccca gcgcctgcgc caccagcgcc gcgatgcgcc
     1801 cttgcgtcag cccttccgac agcgggtaga ccgcctcgca cagcgtcccc agcgccacgc
     1861 cgccatcctc ggccacatgc tcggggtgga cgatctgcag ggtctgccca aactgatcca
     1921 gccgcccggc tatccatcgc ggctcgccca gcggcagctg cttcctggcc cagccgccgt
     1981 tgcgcccgaa ataggtgagc gcacagatat tgcccgccgc atcctcggca tggacgcgga
     2041 acggcgcccg cgccgatccg cccgagcggt attcgcgcgg ggtcagccgc accacgatct
     2101 gctcgcccac gcccgcctcg tcgaggtcgg tcaccgcgcg gcgctgcacg aaccggtcgg
     2161 gcaggtgata ggccaggtcg cgcacccggg tcagccccag cttgtcgagc gggcgcatga
     2221 tccccgcgcc cacgcccttg aggccggcgg tctcggcaaa cagcggattg agccggtcgg
     2281 gacgcatcgc tgcggttcat agccgctcgg cgcgaattcg ccagccgccg acatccattt
     2341 ccgcccgatt tgacccagcg catggaagcg gccggcaatc ggtgctgcac ctgatccgca
     2401 agagaaaagg agtcaccgat gctgctgccc cacggaacca ttgtcgccct ggtcgacggg
     2461 cacaatttcc acgtcttccg caacgccggc aacgaggcca cgcctgaact ggaagcactg
     2521 cccgcccctg cgctcgacag tcacaaccat tccggaacag gccaccattc gcgcccgggc
     2581 aaccacgccg gcacgctcgt cgccgaagat gcccacgcca ttgccgcggt gaactggctc
     2641 aatgaccagg tgctcgcgca caagctcgaa cacctcatcc tgatcgccgc cccgcgcacc
     2701 ctgggcgaaa tgcgccgcca ctatcacaag cagctcgaac aggtcttgat gggcgagctg
     2761 gccaaggacc tctccggccg gaaggggccg gagattctcg ccgccctccg aggcaagtaa
     2821 gcaggggcac gccggaggag accagagcgc cacgcaccct gcccctcctc cggcgtgact
     2881 tgccggaaac cgcgcgatgc cctaggcgcg cggccatgga cgagccccgc ctcaaacgcc
     2941 tccagttccg cgcctggcac cgcggcacgc gcgaggccga ctacatgatc ggctgctact
     3001 tcgaccggtt ccatggtgaa tggggcgaag ccgaaatcgc ctggtttgag gccctgatcg
     3061 aagaggaaga tgtcgacatc atgggctggg cgcttggtac cctggcagtg ccggaagaat
     3121 tcgccggccc gctgatggac cggatgaagc tgctcgacta cgtcgaaatc ccgcgctgag
     3181 ccgtccgcgc ggctcccgtt agcaaatcct ttgcagcgtc ttgctagagc cgtgcccgat
     3241 ggcatggctg gaccgtctcc cttctgaacg cgacagcggc agcacccttg cggcgccgcc
     3301 tggtgccgtg ctgcgtcgcc tatccgttgc cggcctcggc gaaggcgccg tccgcgccca
     3361 gtgggacgat ctcgccgccc gcgcggccga gcctaatccg tttctcgaaa gctggtttct
     3421 gactcccgct ctcgccgcgc tggatccggc tggcgcggcc gaggtcatgg tgctggaagc
     3481 tggcggtcgc tggcttggcc tgctccctct cgcccgcctc cgccgctact acgggcgacc
     3541 gctgccccac cttggcaact ggagccacgg caacgcattc cttggcgctc cgctggtggc
     3601 ccggggcgca gaagctgcct tctggactgc cctcctcgcc catgccgacg gcgcggcagg
     3661 cccagctctg ttcctccacc tgaccgacca gccgcttacc gggccgctcg ccatcgcgct
     3721 gatgcgggtc tgcgccgggc agcgtcgccc catcggcctc gtccaccgcg aggagcgggc
     3781 tctgctcgcc tccgacctct cgcccgaggc ctatctcgag gccgcgttga ccggcaagaa
     3841 gcgcaaggaa ctgcgccggc agcgcgctcg cctctccgac cagggcgcgc tggccttcgc
     3901 ccgcaagcgc gacgaggctg gccttgccga atggacccgg gacttcctcg cgctcgaagc
     3961 tgcgggctgg aagggccgcg ccggctcggc cctcgctgcc gacccggcca cgacggccct
     4021 gttcgccgcg gcgctcgaag gctccgctgc ccggaaccgc ctcgaacggc tgaccctcag
     4081 cctcgacgga acgccggtcg ccatgctcgc caccttcctg gccccgccgg gcgccttcgc
     4141 cttcaagacc gccttcgccg aagacttcgc ccgctattca cccggagtcc tgctccagcg
     4201 cgaaaacctt ggcctcctgg ccgacccgga cctggcctgg tgcgattcct gcgcggccgc
     4261 caatcatccg atgatcgatc acctctggcg cgagcgccgc gccatcggcc ggttctccgt
     4321 cgcgatcggc ggccgcgcgc gacgcgccgc gttctcgctg ctgctgaaag ccgagatggc
     4381 ccgctcggga agccaggcat gaccgtgttc ccgcctaccg ccctcgccac cttcgcgcag
     4441 aactatcccg aagttccgca caagcttgtc cacaccctcg gcagccaccc gctgctcgat
     4501 ctggaagccc tggccgagct ggccgaggcc ctccccgccg ccagtgtcga atacaactcc
     4561 gccgacctgc cgatcggcat agaccgcaag cccgaccgca ccggcatccc catcggcgaa
     4621 accattcgcc gcatcggcga atccggctca tgggccgcgc tcaagaacat cgagcaggtg
     4681 cctgcctatg ccgatctgct ccacagcctc ctggccgaac tgcgcgcggc catcgaaccg
     4741 cgcacggggg ccatgctgcg gctcgaaggt ttcatattca tcacctcgcc aggcggcgtc
     4801 acgccctacc acttcgatcc ggagcacaac attctcctcc aggttcgcgg caccaagacg
     4861 atgacccagt tccccgccgg cgacccgcgc tatgcgccgg acgcggtcca cgaggcctat
     4921 cacaccggcg gcggacgcga actgcgctgg gaggatggcc tgctgcccgg cggcaccgag
     4981 tttgcactgg ctcccggcga ggctctgttc gttccggtca tggcgcctca tttcgtccgc
     5041 aacgggcccg aatcgtcggt gtcgctctcg atcacgtggc gctcgaaatg gtccttcgcc
     5101 gaagccgacg cacgtgccct caacaaggtg ctccggcgct tcggcctgac gccccgcgca
     5161 cccgcccgct ggcccgcctc caaccgcgcc aagtccatcg gatggcggat gctccgccgt
     5221 tttggactgg ctccgggctg accggcaggg ctgacagccc aagttataca tgaatgtata
     5281 attatgattc cgaattttga cctgccgcaa tgatagcgga tcgaccttgt ggaactcctg
     5341 cgggcagaca accgccaacc ggagaggccg atcatgaccg ccaccacagt gcccgatctc
     5401 agcacccgca ccgacttcat ccccaaggaa gactacatca gccccgaatt cgcgaaaggc
     5461 gagaaggaac ggatgtggcc ggcttcgtgg ctgatggcgt gccgcgagga agaccttgcc
     5521 gagccaggcc agttctacac cttcaacatt gctgatgact cgatcatcat caaccgcgac
     5581 gaggccggac gaatccaggc ccaccacaat gtctgccccc atcgcggccg gcgcctgacc
     5641 gcgggctgcg gccatgccac caaggggttc tactgcaact accacggctg gcagtggaac
     5701 ctcgacggct cgaacaaggt aatgatcgat ggcgaggaat ggagcggcat cggccaggcc
     5761 gacgtcggcc tgcgcccggt ccgggtcgac acctggggtg gcttcgtgtt cgtgaacatc
     5821 gatggcaagg ctgatcccct gctcgactgg ctgcaccctt ggccggaaat gtgggagagc
     5881 taccgcatcc aggacatgcg gtactgctgg tccaagagca cccgcctcga ctgcaattgg
     5941 aaggtggcga tcgaggcctt cctcgaaggc taccacgcgc agaccacgca ccgccaggtc
     6001 aacaattgcg acggcggcag ccattcgatc tgccagacct tcggtcggca cgcgatgttc
     6061 tcgatgtacc aggtggggca tctcggcgag cgcagccaca acgccaagta cctgcccccg
     6121 tacaattccg acctgttcga aggggtcacg gatctgcgtg agcgcatcgc cctctacatg
     6181 aaggtg
//
LOCUS       DATNBV010000010         2838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1605104,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000010 DATNBV010000000
VERSION     DATNBV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605104"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VN627_00230"
     CDS             complement(<1..217)
                     /locus_tag="VN627_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01710.1"
                     /translation="MASAPLGISSVQSPTADFAYGAPARSPAHEQPGRAPALAIYVDR
                     PHLREQVREDALVAGFRVAACQSLAALG"
     gene            603..1724
                     /locus_tag="VN627_00235"
     CDS             603..1724
                     /locus_tag="VN627_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HXD01711.1"
                     /translation="MRWVRNAAIVATALLGMVLTGEAAAARPVTDCPMRDTPFSADSP
                     LVDLLLSPAAKAVLDQHGARSLAKLPPIFGSTTPPTFTAILTLRTANMLTGLKPSAMP
                     EIDAQLRALPLTQADRIARCARYDNEVPAFTLDPGKRHLLLFEKINGFKDVPSVDAAH
                     KAFADMAARKGWDIVATDKGGAINPATLKRFDAVIWNNISGDVLTLAQRKALRDYLNA
                     GGGFVAVHGSAGDPAYFWEWYADRLIGARFIGHPMGPQFQEARIVANKAHPLAKALPA
                     EWRMTDEWYSFSTDPRRVGAKVVLTLDESTYKPVGTAGADLRMGDHPLAWTNCIGPRK
                     RGRMFYSAIGHLPATYGRAEYVTLLEAAIDWASTRAACR"
     gene            complement(1854..>2838)
                     /locus_tag="VN627_00240"
     CDS             complement(1854..>2838)
                     /locus_tag="VN627_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXD01712.1"
                     /translation="YAVSSAVLPVRKFKQGTYNLGLNWQITPSAMVYFATRRGYRAGS
                     YNSPLYDAFVGNSQVFGPETLTDYEIGTKLRWESGGMSGSFDFAGYIGKDKNVQVAVP
                     TSQLSGSSLACIAEAFNFPGSTAPLCSTSGPGGTLDGRQVGIPNPTTYTNAGAATISG
                     FEAQATLSPVEGLTLSAGAAYVHVKTDSILLDTNLKTVLVANGREPFIPTQIILRQQP
                     EWTVTGGLYYETPERWILNSKAFVNLDLRYRSSYVEGEDTIRSATSVDARVGLNDIGQ
                     TGLDAAVSVTNVFDKIYDYGSVGSALSSGYHTTLRAAPRTVSLSLRYHWGR"
BASE COUNT          518 a          889 c          923 g          508 t
ORIGIN      
        1 cgccaagcgc ggcgagtgat tggcaggcag cgacgcggaa gccggcgacc agggcatctt
       61 cgcgcacctg ttcgcgcagg tgcggccgat cgacataaat cgccagcgcc ggggcgcgcc
      121 cgggctgctc atgcgccggg ctgcgcgcgg gggcgccata ggcaaaatcc gccgtgggcg
      181 attgcaccga agaaatgccg aggggagcgc tggccatgat caattcgcct ttcgttccaa
      241 tgctgttcca attcgaacag atctagaaca aatacggctc aggtcaagca ctgataagga
      301 acattacgga tgaatctctg accagttgcc ggctggggca actatttgcg aacctttgga
      361 tttttgcgtt cgaccgaggc ttgccgcaag tttggcctcc gaatctacat ccaaaatgga
      421 gggagtttgg tctggagtgg ttcaacgtgg ctgttgctgc agcccatctc cgtgagccag
      481 gcgttccggc ttcggccctg acctcgcgaa atcggtccac accgccagaa gccccttccc
      541 tctcgccgtt tgttcatcca ctggggcgaa acggaagacc gaataaccca aggaattcat
      601 cgatgcgctg ggtacgaaat gcggcaatcg tagcgacagc cctgcttggc atggtgctga
      661 ccggagaagc ggcggctgcc cggccggtga ccgattgccc catgcgcgat accccattct
      721 cggcggattc gccgctcgtc gacctgttgc tgagcccggc ggccaaggcc gttctcgatc
      781 aacacggcgc ccggtcgctg gccaagttgc ccccgatatt cggttccact accccgccga
      841 ccttcacggc gatcctgacc ttgcgcacag cgaacatgct gaccgggctc aagccctcag
      901 ccatgccgga gatcgacgcg cagttgcggg cgctgccgct tacccaggcc gacaggatcg
      961 cgcggtgcgc gcgctatgac aacgaagtgc cggcgttcac cctcgacccg ggcaaacggc
     1021 atctgctgct cttcgaaaag atcaacgggt tcaaggacgt gccttcggtc gatgcggcgc
     1081 acaaggcttt cgcagacatg gcggcgcgca agggctggga catcgtcgcg accgacaagg
     1141 gaggggcaat caaccccgca acgctgaagc ggttcgatgc ggtgatctgg aacaacatca
     1201 gcggcgacgt gctgaccctg gcgcaacgca aggccctgcg cgattacctg aacgccggcg
     1261 gcgggttcgt ggcggtgcat ggttcggcgg gcgatccggc ttacttctgg gaatggtacg
     1321 ccgaccggct gatcggcgcc cggttcatcg ggcatccgat gggcccgcaa ttccaggagg
     1381 cgagaatcgt cgccaacaag gctcaccctc tcgccaaggc actccccgcc gaatggcgga
     1441 tgaccgacga atggtattcg ttctccaccg atccacgccg cgtgggggcc aaggtcgtgc
     1501 tgaccctgga cgaaagcacc tacaagccgg tgggcacggc cggcgccgat ctgcggatgg
     1561 gcgatcatcc cctggcctgg acgaattgca tcggtccgcg caagcggggg cggatgttct
     1621 attcggcgat cgggcatctg ccggccacct acggccgggc cgagtacgtg acactgctgg
     1681 aagccgcgat cgattgggcc tctacgcgcg cggcctgccg ctgaccctgg atcggcgggt
     1741 tccgcaatgc cgccaggtat gatggcgcgt attctgctgg ccctgaacgc aggaacgggc
     1801 ggctccttgc ggaaccgccc gtccttggat ttatccggcc gcagccggga ggctcagcgg
     1861 ccccagtggt agcgcagcga cagcgacacg gtgcgcggtg cggcgcgcaa ggtggtgtgg
     1921 taacccgatg agagcgccga acccaccgat ccatagtcgt agattttgtc aaacacgttg
     1981 gtgacactca ccgccgcgtc gagcccggtc tgaccgatgt cattcaaccc gacccgcgca
     2041 tcgacgctgg tggcgctgcg gatggtgtcc tcgccctcga cgtaggagct gcgatagcgc
     2101 aggtcgaggt tgacgaacgc cttgctgttc aggatccacc gttcgggggt ctcatagtag
     2161 agaccgccgg tgacggtcca ctccggctgc tgccgcagga tgatctgcgt cgggatgaac
     2221 ggttcccggc cgttggccac cagcacggtc ttgaggttgg tatcaagcaa gatgctgtcg
     2281 gtcttgacat ggacgtaggc ggcgcccgcg ctcagcgtca ggccttcgac cggcgacagg
     2341 gtagcctggg cttcgaagcc cgagatcgtg gcggcaccgg cgttggtata ggtcgtcggg
     2401 ttcggaatgc cgacctgacg gccatcaagc gtaccgcccg gaccgctggt gctgcacagc
     2461 ggcgccgtcg agcccgggaa gttgaacgcc tcggcaatgc aggctagcga cgaacccgat
     2521 agctgcgagg tcggcacggc cacctggacg ttcttgtcct tgccaatata gccagcgaaa
     2581 tcgaagcttc ccgacatgcc gccgctttcc cagcgcagct tggtgccgat ttcgtaatcg
     2641 gtcagcgttt ccggcccgaa tacctggctg ttgccgacga aagcgtcgta gagcggggag
     2701 ttgtagctgc cggcacgata gccgcggcgg gtcgcgaagt agaccatcgc cgacggcgtg
     2761 atctgccagt tgaggccgag attgtaggtg ccctgcttga atttgcgcac cggcaggacc
     2821 gccgaactga ctgcataa
//
LOCUS       DATNBV010000011         4729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_646267,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000011 DATNBV010000000
VERSION     DATNBV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646267"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3417
                     /locus_tag="VN627_00245"
     CDS             <1..3417
                     /locus_tag="VN627_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01713.1"
                     /translation="GYNNGIVPFKSYMFGESYTREGLPAKLVSPGSPPGTLTKRQAAR
                     GALAAMYPLPRWNVIPPGDIFRVFERGGRTIGSGFPEIGLPNSSGSIQRLEEPGRPDL
                     KQSNRGPGTGLRSAIPVLNIHKTRLNDPFLWFMGTNDQPGDYRGSGCTGCHVIYANDR
                     EPRHSLTYAQYGRDGQTITVDPTIADKREPISSGHGALKDGHEGEGTEKEHGHPLQHV
                     FTRSIPTAQCMNCHMHQPNIFLNSYLGYTMWDYESDAPKMWPEHQKYPTAAEVRATND
                     RNPEGAAAHGKWGDLDFLRNVYDLNPTLKDTQFADYHGHGWNFRAIFKRDREGNLLDA
                     EGKIVSPDDPEKWRKAGEGKFVPPGVNPGKTVHLMDIHAEKGLQCADCHFAQDSHGNG
                     LIYGEVANAVQIGCKDCHGTADALPNLRTSGPAAPPKGEDLSLLRNPDGQRRFEWQDE
                     GGRRVLIQRSIVDPKLEWRVHLVKDSVDPTSPYFNAKAARAKLMSRSGADDGLFRFGP
                     GVAPGDRAHDPDKMACFTCHLSWTTSCGGCHLPIEANWKTKMHHFEGEETRNFATYNP
                     QVARDEMFQLGRHMTTKGAEIAPIRSTSALVLSSTNINRERIYIQQPPISSIGFSSQA
                     FAPHFPHTVRTTETKTCTDCHLSAADDNNAIMAQLMLLGTNFVNFVGLNAYTGLEGGL
                     QATRVTEWGEPQAVIGSYLQKYAYPDFYRLHVEQNHRELKNWTRGKTLDGKLSGETDP
                     LEQFRNVTHGTPGAVGCLQLRGEYLFVAEGKGGFMAYDVASIANKGISEPILSGPSSP
                     LGHDTGVKTTNATCMAIPTNQAIAPTRNTPELRALNQEQPFLPIYSYAAVTDSVEGLV
                     MVNVDTLADGELRNNKLKRAVTWNPDNVLKGARHIQLAGEIAYITADVGLVVVDLHDP
                     LHPKLAAVRPLRDARASAIQFRYLWVTDAEGLKLFDVTDLRNPVAMPQGTVPMADAHH
                     LYLARTYAYVAGGAEGLVIVNITRPLAPQVYQKVTFGGTMNDVQDVIVGTTNASLFAY
                     VADGRNGVKVIQLTSPDSQPNFYGFSPAPRPELIAWARTPSPALSMSKGLDRDRGVDE
                     TGGQIAVFGRLGSRPFTRAEMEALFLNKRGMPFRVTDEPDMSRWVAASTH"
     gene            complement(3628..>4729)
                     /gene="zapE"
                     /locus_tag="VN627_00250"
     CDS             complement(3628..>4729)
                     /gene="zapE"
                     /locus_tag="VN627_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HXD01714.1"
                     /translation="VAAGELKPDAEQLAAAQRLAQLQTDLEAPAPTGGILGRLLARKP
                     AARPRGIYMWGGVGRGKSMLMDLFHDTLRIAEKRRAHFHAFMLEVHALLREERKKESG
                     DPIPPVAATIARGLKVLAFDEMVVNNSADAMIMSRLFTALIMSEGVTVVTTSNRAPGQ
                     LYKDGLNREHFLPFIALIERELDVLTLNGPTDYRLDRLTGIDTWYCPLGEEATARARE
                     AFFRLTDYPPEDSAHVPSGELDLGGGRTLFVPKSFKGVAVFSFRRLCEEARGAPDYLA
                     VAQAYHTVIIMGIPRLGPERRNETARFKVLIDALYEARVKLIAAAATRPEELYDPGPG
                     GDEDGRFEFDRTVSRLMEMQSRDYLALGHGEE"
BASE COUNT          849 a         1788 c         1378 g          714 t
ORIGIN      
        1 ggctacaaca acggcatcgt cccgttcaaa tcctacatgt tcggcgagag ctacacccgc
       61 gagggcctgc ccgccaagct cgtctccccc ggctcacctc ccggcacgct gaccaagcgc
      121 caggccgccc gcggcgcgct cgccgcgatg tatccgctgc cgcgctggaa cgtgatcccg
      181 ccgggtgaca tcttccgcgt gttcgaacgc ggcggccgca ccatcggctc gggcttcccg
      241 gaaatcggcc tgcccaactc gtcaggctcg attcagcgcc tggaagagcc cggccgcccc
      301 gatctcaaac agtccaaccg cggccccggc accgggctcc ggtctgccat cccggtcctc
      361 aacatccaca agacccgcct caacgatccc ttcctgtggt tcatgggcac caacgaccag
      421 ccgggcgact atcgcggctc gggctgcacc ggctgccacg tgatctacgc gaacgaccgc
      481 gagccgcgcc acagcctcac ctacgcgcaa tatggccgcg acgggcagac catcaccgtc
      541 gatcccacca tcgccgacaa gcgtgagccg atctcatcag gccacggcgc gctcaaggac
      601 gggcacgagg gtgaggggac cgaaaaggaa cacggccacc ccctccagca tgtcttcacc
      661 cgctcgatcc ccacggccca gtgcatgaac tgccacatgc accagcccaa catcttcctg
      721 aattcctacc tcggctacac catgtgggac tacgagtccg acgcgccgaa gatgtggccc
      781 gaacaccaga agtatcccac cgccgccgag gtccgcgcca ccaacgaccg caaccccgaa
      841 ggcgccgccg ctcacggcaa gtggggcgat ctcgatttcc tgcgcaacgt ctacgatctc
      901 aaccccacgc tcaaggacac ccagttcgcc gactatcacg gccatggctg gaacttccgc
      961 gcgatcttca agcgcgaccg cgagggcaac ctgctcgatg ccgagggcaa gatcgtctcg
     1021 cccgacgatc cggaaaagtg gcgcaaggcg ggtgaaggca agttcgtgcc ccccggtgtc
     1081 aaccccggca agaccgttca cctcatggat atccatgcgg agaagggcct gcagtgcgcc
     1141 gattgccact tcgcgcagga cagccacggc aacggcctga tctatggaga ggtcgccaat
     1201 gccgtgcaga tcggctgcaa ggattgccac ggcaccgccg atgccctgcc aaacctgcgc
     1261 acctccggtc ccgccgcccc gcccaagggc gaagacctct cgctcctgcg caaccccgat
     1321 ggccagcgcc gcttcgaatg gcaggatgaa ggtggccgcc gcgtcctgat ccagcgttcg
     1381 atcgtcgatc ccaagcttga atggcgggtc cacctcgtca aggattcggt cgatcccacc
     1441 tcgccgtatt tcaacgccaa ggccgcccgc gccaagctga tgagccgctc gggcgcggac
     1501 gacggcctct tccgcttcgg gccgggcgtg gccccgggcg accgcgccca cgatcccgac
     1561 aagatggcct gcttcacctg ccacctctcg tggaccacct cgtgcggcgg ctgccacctg
     1621 ccgatcgagg ccaactggaa aaccaagatg caccacttcg agggggagga gacgcgcaac
     1681 ttcgcgacct acaaccccca ggtggcgcgt gacgagatgt tccagctcgg ccggcacatg
     1741 accaccaagg gcgccgagat cgccccgatc cggtcaacct cggcgctggt gctgtcctcc
     1801 accaacatca atcgcgaacg gatctatatc cagcagccgc cgatcagctc gatcggcttc
     1861 tccagccagg ccttcgcccc ccacttcccc cacaccgtgc gcaccaccga gaccaagacc
     1921 tgcaccgatt gccacctctc ggcggcggac gacaacaacg cgatcatggc ccagctcatg
     1981 ctgctcggca ccaatttcgt gaacttcgtc gggctcaatg cctacaccgg cctcgaaggc
     2041 ggcctccagg cgacccgcgt caccgaatgg ggcgaacccc aggccgtgat cggctcctac
     2101 ctccagaaat acgcctatcc cgatttctac cggctccatg tcgaacagaa ccaccgcgag
     2161 ctcaagaact ggacccgcgg caagacgctc gacggcaagc tctcgggcga aaccgatccg
     2221 ctcgagcagt tccgcaacgt cacccacggc acccccggcg ccgtcgggtg cctccagctg
     2281 cgcggcgaat acctgttcgt ggcggaaggg aagggcgggt tcatggccta tgacgtcgcc
     2341 tcgatcgcca acaagggcat ttccgagccg atcctgtccg gcccgtcatc gccgctcggc
     2401 cacgataccg gggtgaagac caccaacgcc acctgcatgg cgatcccgac caaccaggcc
     2461 atcgcgccca cccgcaacac gcccgaactg cgcgcgctca accaggaaca gccgttcctg
     2521 ccgatctact cctacgccgc cgtcaccgac agcgtggagg gcctggtcat ggtcaacgtc
     2581 gatacgctgg ccgatggcga gctgcgcaac aacaagctca agcgcgccgt cacctggaac
     2641 ccggacaacg ttctcaaggg cgcgcgccat atccagctcg ccggcgagat tgcctatatc
     2701 accgccgatg tcggcctcgt cgtcgtcgat ctccacgatc cgctccaccc caaactcgcc
     2761 gccgtgcgcc cgctcaggga tgcccgcgcc agcgcgatcc agttccgcta cctgtgggtg
     2821 accgacgccg aaggcctcaa gctgttcgac gtcaccgatc tcaggaaccc ggtcgccatg
     2881 ccgcagggca ccgtgcccat ggccgatgcc catcacctct atctcgcccg cacctatgcc
     2941 tatgtcgcgg gcggggccga ggggctggtc atcgtcaata tcacccggcc gctggcgccg
     3001 caggtctacc agaaggtcac cttcggcggc acgatgaacg atgtgcagga cgtcatcgtc
     3061 ggcaccacca acgccagcct gttcgcctat gtcgccgatg gccgcaacgg ggtgaaggtc
     3121 atccagctga ccagcccgga cagccagccc aatttctacg gcttctcgcc cgcgccccgg
     3181 cccgaactca tcgcctgggc gcgcacgcca agcccggcac tgtcgatgtc gaaggggctc
     3241 gatcgcgatc gcggcgtcga tgaaaccggc ggccagatcg ccgtattcgg ccgcctcggc
     3301 tcgcggccct tcacccgggc ggaaatggaa gccctgttcc tcaacaagcg cggcatgccc
     3361 ttccgggtaa cggacgagcc cgacatgagc cggtgggtcg cggcgagcac ccactagcgc
     3421 aagccgccat accctcgcca ccctgcaccc gtcgcccgtg cgttggcagg ggcctatccc
     3481 agcctcgcgt gtaaatacca ccgctgggtt cgggtgcgag atcaggatgg ggccctgcct
     3541 acccagaggc gacggcgcag cgaccgatgg tgtagggaac gacggtacgg gcagcggttt
     3601 tcggcgcgaa accgcagaga gcgaacgcta ttcttcccca tgccccagcg ccaggtaatc
     3661 ccggctctgc atttccatca gccggctcac cgtccggtcg aactcgaacc gcccgtcctc
     3721 gtcgccgccc ggacccggat cgtagagctc ctccggccgc gtcgccgccg ccgcgatcag
     3781 cttcacccgc gcctcgtaca gcgcatcgat cagcaccttg aagcgcgccg tctcgttgcg
     3841 ccgctcgggc ccgagccgcg ggatgcccat gatgatgacg gtgtgatagg cctgcgccac
     3901 ggcgaggtaa tccggcgccc cgcgcgcttc ctcgcacagc cgcctgaagc tgaacacggc
     3961 cacccccttg aagctcttgg gcacgaacag ggttcgtccg ccccccaggt ccagctcgcc
     4021 gctgggcacg tgggcgctgt cctcgggcgg gtagtcggtc agccggaaaa acgcctcgcg
     4081 cgccctggcc gtggcctcct cgcccagcgg gcaataccag gtgtcgatgc cggtcaggcg
     4141 gtccagccgg taatccgtag gcccgttcag cgtcagcacg tcgagctcgc gctcgatcag
     4201 ggcgatgaac ggcaggaaat gctcgcggtt cagcccgtcc ttgtagagct ggcccggcgc
     4261 gcggttcgag gtcgtcacca cggtcacgcc ctcgctcatg atcagcgccg tgaacagccg
     4321 gctcatgatc atcgcatcgg ccgaattgtt gaccaccatc tcgtcgaagg cgagcacctt
     4381 cagcccgcgc gcgatcgtcg ccgccaccgg cgggatcggg tctccgctct ccttcttgcg
     4441 ctcctcgcgc agcagggcat gaacctcgag catgaaggcg tgaaagtgcg cccggcgctt
     4501 ctcggcaatg cgcagggtgt cgtggaacag gtccatcagc atggacttgc cccggcccac
     4561 gccgccccac atgtagatgc cccggggccg cgccgcaggc ttgcgggcca gcagcctgcc
     4621 aagaatcccg ccggtcgggg cgggggcctc caggtccgtc tgcaactgcg ccagccgctg
     4681 cgccgccgcc agctgctctg catcgggctt cagctcgccc gccgcgacc
//
LOCUS       DATNBV010000012        14677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3202303,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000012 DATNBV010000000
VERSION     DATNBV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14677
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3202303"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..624
                     /locus_tag="VN627_00255"
     CDS             496..624
                     /locus_tag="VN627_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01715.1"
                     /translation="MTRLVTSLFGTQGSGWQVGAAGLVLIGAAVTIAYAVVDNFWG"
     gene            complement(594..1502)
                     /locus_tag="VN627_00260"
     CDS             complement(594..1502)
                     /locus_tag="VN627_00260"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HXD01716.1"
                     /translation="MRRLIRPPVLTLAALGLLVAPGLAEAHAGHHHQAPEWTFAPGVV
                     IPLALTLVLYMVGWIRLAHRSSLRMPRHRAGLFLSGWATMAAALVSPLHEAGEHSFTL
                     HMIEHELIMLPAALLLVASRPGPVLLWGLPAPLRQGLSPLLRLPLWRWLAAPLVATGL
                     QAAALVGWHMPALFNRALTSEWWHVAQHASFAGTALLFWWAMLPRRGAESSLVASICL
                     FVTSMVGSGLGALMALSASPWYPAYAAMGAMPFGLSPEMDQQLAGVIMWVPGGLFHLG
                     AALILLARSLMGTPSRRQPQKLSTTA"
     gene            complement(1490..1819)
                     /locus_tag="VN627_00265"
     CDS             complement(1490..1819)
                     /locus_tag="VN627_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01717.1"
                     /translation="MPRTDLIKRPQPFMALLLVAGGWALSHQLGSDSVFDDCLSRGGP
                     FVVLASVVGLVITAAGAIYAAASWRAPEESGRSFLAVITMLLAPIAGFAMVMQLAAGL
                     ILPRCAA"
     gene            complement(1821..2453)
                     /locus_tag="VN627_00270"
     CDS             complement(1821..2453)
                     /locus_tag="VN627_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HXD01718.1"
                     /translation="MASTAPSPQPNFALDLEPLETHAFGNRSLTWWGIVAFFLIEGTA
                     FALAIAAYFFLMSWDHAWPPPPLNPPDLLAGTLFTIVILLSEIPNTLIKWAAEKYELP
                     RVRGLLVLMSLIGLVLLVVRAFEFNSLNVFWYQNAYGSAIWMLLVLHTTHIVTDWVDT
                     LVLTALMFTPHGTEGRRFVDCSENAVYWRFVWLCWLPIYALIYWLPRLVN"
     gene            complement(2455..4368)
                     /gene="ctaD"
                     /locus_tag="VN627_00275"
     CDS             complement(2455..4368)
                     /gene="ctaD"
                     /locus_tag="VN627_00275"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HXD01719.1"
                     /translation="MSERTYLRDGPDLTGAALHDKLARTWGDRPGFWGWAASVDHKSI
                     GRRYLVTAFVFLALGGVLALAMRLQLSGPQKALIGPDRYNQIFTMHGTTMMFLFAVPV
                     MDAVAIYLLPLMLGTRNLAFPRLNAFSWWMYVAGGSMLWNAFVLNAGPDAGWFSYVPL
                     AGPEFSPGKRVDFWAQMVTFTEVAALAVAVSITATILKARAPGMTLARMPVFAWSMLV
                     VSLMVIFSMPSIALTSAMLMADRMVGTQFFNPYEYGDALLWQHLFWFFGHPEVYIIFL
                     PATGFVSEIVQVFTGRQVFGHTAVVLSLVATGLLAFGLWVHHMFATGLPRMGYAFYTA
                     ASMAVAVPSGIQIFCWIGTIWTGRPRYRLPMLWVVGFIVTFVAGGLTGVMLASVPLDL
                     QVHDTYFVVAHFHYVLIGGAVFPLLGAVVYWFPKFTGRMMDERWGKLAFWIALPAFHL
                     TFFPMHYLGIMGMPRRVYTYPAGMGWDTLNLISSIGAFLFAGGVLVFVGNALWSLKRG
                     EAAPADPWAAHGLEWTTSSPPAPYNFAHIPVVTSATPLWDPERPVTRGLRVEDREVLL
                     TTVFEARPDLREPSPMPTLWPMIAALATTVLFVCSIFTPWAIVIGSVPVGVALIAWFW
                     PKDRGPEGEPVVD"
     gene            complement(4365..5366)
                     /gene="coxB"
                     /locus_tag="VN627_00280"
     CDS             complement(4365..5366)
                     /gene="coxB"
                     /locus_tag="VN627_00280"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HXD01720.1"
                     /translation="MRRGAVLLVMALGGCSALYGPQSSAMGQGMESAHIGNLFDLFLW
                     VTVPIYLGVMVFLALAMRRRQAMVQGAARPEQEAGWRLGLVLFAVTSALILFGLSVAT
                     WFTDRAIARAGAGPALSVEVIGHQWWWEVRYHDPVAGRELRTANELVIPAGATVKVSL
                     SSPDVIHSLWIPNLAGKQDLIPGRTSDVVLKVPKPGEFRAQCAEFCGLQHAQMALPVS
                     VRAPGDFAKWYNAGLATPPVPGGGPEFAGYSLFTGRQCASCHNIAGTPASGQVAPDLT
                     HFGSRTTIASGVLPNTAASLDRWIADPQRVKPGTQMPQVPLSPEERAAVVAYLESLK"
     gene            complement(5363..5800)
                     /locus_tag="VN627_00285"
     CDS             complement(5363..5800)
                     /locus_tag="VN627_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXD01721.1"
                     /translation="MMDSPHRGAKFSGNAWRISNGARLFVWMNCAGCHSHGGGGMGPP
                     LMDAKWRYGSSMADVVYTILNGRPGGMPAFRGKITEDQAWELAAYVRSMSARTRRDAL
                     SGRADEPANVEPLPLEKRRRARAVTPEQDAAQKESSKIEGTGP"
     gene            complement(5871..7748)
                     /locus_tag="VN627_00290"
     CDS             complement(5871..7748)
                     /locus_tag="VN627_00290"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXD01722.1"
                     /translation="MKSIWPVSVLMILLAALVGLGAAVLATTYPAPHATFSDRPVVYG
                     ALPAPQDRARLASWAQPSPDGQWTWQLGDSAASRFSPLDDINARNVAQLKPVFTFETG
                     LKRGHEAGPLVVGDTMYVTTPYPNDVYALDLTRPGAPVKWKYHPQSQKAAQGVACCDV
                     VNRGAVYGAGRLYFNTLDGQTIALDAATGHEVWKAKLAEISKGETITMAPLLAEGRVL
                     VGTSGGEMGVRGSLAALDAASGKLVWRAYSTGPDKDVLIGPEFRPFYKQDQGKDLGVS
                     TWNPEGWKIGGGNPWGWVSYDPELHLIFYGTGNPGPWNPDQRPGDNKWTAGVFARDVR
                     TGQARWFYQTSPHDLFDYDDINESLLLDIPTRQGLRKVVARPARSGFFQIIDRMSGQV
                     LSAKPFAYINSMKGVDLTSGRPIMNPDKEPKEGRTTRNICPAAPGGKDWNPVSFSPRT
                     GLVYIAHNNLCMDEGDVDTNYIAGTPYLGTEARMYPGPGGNRGVFTAWDPVAGRKVWE
                     IREDLPLWSGTLATAGDVVFYGTMDGWFKAIDARTGRLLWRTKLVSGIIGMPMTYKGP
                     DGRQYVAIFAGVGGWSGAIVSGALDPGDPTTALGFVNAMKDLVKRTDAGGRLYVFALP
                     R"
     gene            complement(7934..8737)
                     /locus_tag="VN627_00295"
     CDS             complement(7934..8737)
                     /locus_tag="VN627_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXD01723.1"
                     /translation="MSRITLLAGAVGSLLLLGSCSALSRKPLRVCADPNNLPFSNRAG
                     AGFENKLAELVATDLHRPLQYVWWAQRRGYVRNTLNAAQCDLWPGVASTVDMMATTRP
                     YYRSTYVFVTRRSAGLGGLTLDDPRLRKLRIGVQMIGDDGMNTPPAHAIARREIIDNV
                     RGYMVYGDYREANPPSRIVRAVADGEIDVALAWGPVAEWYARTSTVPLRIEPVTPWLD
                     QAQWPMAYDISMGVQKHDQKLRASLDAVLARRRSEIAGLLASYGVPLVQ"
     gene            complement(8734..9693)
                     /locus_tag="VN627_00300"
     CDS             complement(8734..9693)
                     /locus_tag="VN627_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated
                     beta-propeller protein"
                     /protein_id="HXD01724.1"
                     /translation="MLALPLLLGACQSQTASTDTVFVSNEASGVVHVLDGASGKSVGA
                     LATGARPRGLALSPDGQVLYVAASKANRIEAWSVKERRRLAMWSSGSDPERFAVSPDG
                     KRLYIANEDHSAVSFLDLPSGKIIAEVKVGPEPEGMGVSPDGRLVVATAEASSTVHFI
                     DATSGRLLVSAPVGNRPRGVLFVGGGRQVWISSEQRGLIAVFDAATLQRTGTIDLVPD
                     FPEAEQVQAVEMHATPDGRRVFVSMGRTNRVAEIDPATGKVVRSFATGERTWGLAISP
                     DGKRLYAASGLSGTLTTVDLVSNRVVRTDQVGGRPWGVVAARK"
     gene            complement(9837..10745)
                     /locus_tag="VN627_00305"
     CDS             complement(9837..10745)
                     /locus_tag="VN627_00305"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXD01725.1"
                     /translation="MQSVRVPPIKQPGDLILAKLHVWANEFFLQVPNIAAAAVFLLLA
                     WGLARTAAWGVRKLLSRRERIDLGELLASIAFGLIMLLALMVAAAILFPSVKPADILA
                     TLGIGSVAIGFAFKDILQNLFAGLLLLTRRPFRRGDEIAVKDYEGTVEHIESRATLIR
                     TYDGRRVIIPNADIYTGVVVVNTAFATRRDEADFGIGYNDDLAGAMTFVTDTIRRIDG
                     VETEPGPEVIAVEFRDGDVRLRARWWTRSARSEAVHIRSRVVEAILQAFRAHGVDLPF
                     PTQIVLLHDRSGQNEDDRSEQRLRSS"
     gene            10923..11300
                     /locus_tag="VN627_00310"
     CDS             10923..11300
                     /locus_tag="VN627_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01726.1"
                     /translation="MDGTQFAALRWRSMKPACIGIALALVGCGSQSRSECEALRTTES
                     VLNELAWSAEQADAGRTPRTYLEAFQRQGVQQLKQSAQELRPHDPTAAGIVDGVAGYA
                     GTPDRLRAAARSLRAREATLEAA"
     gene            11287..12522
                     /locus_tag="VN627_00315"
     CDS             11287..12522
                     /locus_tag="VN627_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HXD01727.1"
                     /translation="MKPLELTLGILTAVGGFVDISELVFVSQAGARFGYALIWVIVLA
                     TVGIIVFGEMSGRIAAVSRQPMFTIIRKELGLTLGGVTLIGGLVSTLVTCAAEIGGVA
                     LVLQLLTGGSYLLLAALATTVFAASIWLLPFKWIERSYGLLGLVMLVFAAALLALDPP
                     WSHIAAGLVPRVPAGLSQQDMLVYGYFVVALISAIMFPYELYFYSSGGVEDAWSKADL
                     STNRVTSIVGMSLGALMAIVLLCLAAQELGPRGISPEQVGAVVLQPGLAFGKLGVILG
                     LLGILTAVAGAAIETCLANAYAVSQFFGWRWGRHRPPQDTPLFTLAWLVVFALALALV
                     CTGVEVTDLADYAVVLSIVVLPLSFLPLLLAGNDPVLMRDQVNGPWSRRLGWLFYGLV
                     VLAAVAAVPLYLLTLGGKL"
     gene            12519..12854
                     /locus_tag="VN627_00320"
     CDS             12519..12854
                     /locus_tag="VN627_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01728.1"
                     /translation="MRAADPHHIADAFLDMPLVDLHDHSCGVVDDIELAEIEPGIWEL
                     AAILVGPGAWRRRRPRWLTALLPKLKAVRIAKADIASATQVVRLMRPAAELGLATTER
                     RLLALMGGR"
     gene            13002..>14677
                     /locus_tag="VN627_00325"
     CDS             13002..>14677
                     /locus_tag="VN627_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HXD01729.1"
                     /translation="MGEFDHLDALDALKYAEESEAVRRLLADPVLDPSHSAAVAADAI
                     ELICAARRIGERHGVVQRFLREYSLASREGLALMCLAEALLRTPDTATRDRLIAEQVA
                     SAEWVRHLGHSRSLFVNASTWALMLTGRLFEPDAEARENPGAFLHRLAARLGEPVLRA
                     AVAKALRIMGGQFVLGADIGSALARAKKEHLLCSFDMLGEGARTVADADRYFRRYATA
                     IQAVGEAACGSGPELGHGISVKLSALWPRCEAAHEARVWDELYPRLRHLARAAADFGL
                     NLTIDAEEADRLVLGLKLFDRVAGETELGDWRGLGLAVQAYQPRAVAVIGEVIRIARR
                     RHRRLIVRLVKGAYWDTEIKRAQVSGMSGYPVFTTKPATDVNYLACARELLAAGEAIY
                     PQFATHNAHTLAAVRQLAAKVGRGIEFQRLHGMGEALYEAAQARWGALTVRTYAPVGE
                     HEQLLPYLVRRLLENGANSSFVHRLVDEEIAPSEVAPDPYRQLAVSPGPNSNIPLPRL
                     LYGEGRLNPLGRDLSVASVRENAAAAVASLRTETLTAGPIIAGRQLLDDGR"
BASE COUNT         2597 a         4907 c         4757 g         2416 t
ORIGIN      
        1 gaattccgct tcgccatgaa ttccgtgcag gaccgcaagc gcaatcgagg gcgatacttg
       61 aggtatcggg gatggtcgcg gcggaagaag gaagctatct ggcggtgacc aggtgagtgt
      121 tggacatgac aggtgcgttc cgccgacaca tgggtcgttc cggggtgagg cggcctcctc
      181 aaaagccgtc atccattcac atcttagctg gcggcgtgaa aggtccaagt cggggacttt
      241 cctgccgttc tcccgggatc cgtcaaacgg cagtgttttc caaaagcgga tgtcagttcg
      301 ttgagctctg tatggccgca acgtggggca atctataacc gcggattttg ccgcccttcg
      361 ttcacctccg tccctgtacg cggagaagtg cgtcgactca gaaaatttca ggccccggct
      421 gcataatccg gccctgctcg aagccgagca cctccagaaa cggaaacccc cgccaccggc
      481 cggagttgtg tgggtatgac ccgtctcgtc acatcgctgt tcggcaccca gggctccggt
      541 tggcaagttg gcgcagcagg gctcgtattg atcggcgcag ccgtcaccat cgcctacgcg
      601 gtggtggaca acttctgggg ctgacgccgg gacggtgtcc ccatcagcga ccgggcgagc
      661 aggatcagtg cggccccgag gtggaacaga ccgcctggca cccacatgat cacgcccgcc
      721 agttgctggt ccatttccgg ggagaggcca aacggcatgg ctcccatggc agcataggct
      781 gggtaccaag ggctcgccga aagcgccatc agcgctccga gcccgctacc gaccatcgag
      841 gtcacgaaca ggcagatgga cgccaccaga ctgctttccg cgccgcgtcg cggcaacatg
      901 gcccaccaga aaagcagcgc cgtgcccgcg aacgaggcat gctgcgccac gtgccaccat
      961 tcggaagtca gcgcccggtt gaacagggcc ggcatgtgcc agccgaccag cgccgcggcc
     1021 tgcagtcccg ttgccacgag cggcgcggcg agccagcgcc acagcggcag ccgcagcaag
     1081 ggggagaggc cctgacgcag cggggccggc aggccccaca acagcaccgg gcccgggcgc
     1141 gaggcgacaa gcaacagcgc ggccgggagc atgatcagct cgtgttcgat catgtgcagg
     1201 gtaaacgagt gttcgcccgc ctcgtgcagc ggcgagacca gcgctgcagc catggttgcc
     1261 caaccggaga gaaacaatcc ggcgcggtgg cgcggcatcc gcagtgagga gcgatgggcc
     1321 aaccgtatcc agcctaccat ataaaggacc aatgtcagcg ccaaggggat gaccacaccc
     1381 ggagcgaagg tccattccgg cgcttggtga tgatggccgg cgtgcgcctc ggcaaggccc
     1441 ggcgcgacca gcaggcccag cgccgccagg gtcagtacgg gcgggcggat caggcggcgc
     1501 atcgcggcag gatgaggccg gcggcaagct gcatgaccat ggcgaatccc gcgatgggcg
     1561 cgagcagcat ggtgatcacc gccagaaacg agcgtccact ctcttccggc gcgcgccagc
     1621 tcgccgccgc atagatcgcc cctgccgcag tgattaccag ccccaccacc gaggccaaga
     1681 cgacaaaggg cccgcctcgg gagaggcagt cgtcgaacac gctgtcggag cccagctggt
     1741 gcgagagcgc ccagcctcca gccaccagca gtaaggccat gaaaggttgc gggcgcttga
     1801 tcagatcggt gcggggcatg tcagttcacc agtcggggga gccagtagat cagtgcgtag
     1861 atcggcagcc agcacagcca gacgaaccgc cagtagaccg cgttctccga gcaatccacg
     1921 aagcgtcgcc cctctgtccc atgcggcgtg aacatcagcg cggtgaggac caaagtatcg
     1981 acccagtcgg tcacgatgtg ggtggtgtgc agcaccagca gcatccagat cgccgagcca
     2041 taggcgttct ggtaccagaa cacgttgagc gaattgaact cgaacgcgcg caccaccagc
     2101 aacactagcc cgatcaggct catcagcacc agcaggcccc gcacgcgcgg caactcatac
     2161 ttctccgcgg cccactttat cagggtattg ggtatctcgc tgagcaggat gacgatggtg
     2221 aacagggtgc ccgcgagcag gtcgggcggg ttcaacggcg gcgggggcca ggcgtggtcc
     2281 cagctcatca ggaagaagta ggctgcgatc gcgagcgcga aggcggtgcc ttcgatcaag
     2341 aagaaggcga cgatccccca ccaggtcagc gagcgattgc caaaggcgtg agtctccagc
     2401 ggctcgagat cgagcgcaaa gttcggctgc ggcgaaggtg cggtgctggc catgtcagtc
     2461 taccaccggc tcaccttcgg gcccgcggtc cttgggccag aaccaggcga tcagcgccac
     2521 cccgaccggc acagatccga tcacgatcgc ccacggcgtg aagatcgaac agacgaacag
     2581 caccgtcgtc gccagggctg cgatcatcgg ccacaacgtg ggcataggcg aaggctcgcg
     2641 caggtcgggc cgggcctcga acaccgtggt cagcaggacc tcacggtcct ccacgcgcag
     2701 gccgcgggtc accggccgct ccgggtccca cagcggcgtc gcgctggtca cgaccggaat
     2761 atgggcgaaa ttgtagggcg cgggcggcga gctggtcgtc cattccagcc catgcgcggc
     2821 ccagggatcg gccggcgccg cctcgccacg cttcagcgac cacagcgcgt tgcctacaaa
     2881 caccagcacc ccgccggcaa acaggaaggc gccgatactc gaaatcaagt tcagcgtgtc
     2941 ccagcccatc cccgcgggat aggtatagac ccggcgcggc atgcccatga tcccgaggta
     3001 atgcatcggg aaaaaggtca ggtggaaggc gggtagggcg atccagaagg ccagcttgcc
     3061 ccagcgttca tccatcatcc gcccggtgaa cttggggaac cagtaaacca cggctcccag
     3121 cagcgggaac accgctccgc cgatcagcac gtagtggaag tgggcgacaa cgaagtaggt
     3181 gtcatgcacc tgcaggtcca gcgggaccga tgccagcatc acgccggtga ggccacccgc
     3241 cacgaaggtg acgatgaacc ccaccaccca tagcattggc agccggtagc ggggccgccc
     3301 ggtccagatc gtcccgatcc agcagaagat ctggatgccc gagggcacgg ccacggccat
     3361 ggacgccgcc gtatagaaag catagcccat ccgtggcagg ccggttgcga acatgtggtg
     3421 aacccacagc ccgaaggcga gcagcccggt cgccaccagc gagagcacca ccgccgtgtg
     3481 cccgaatacc tgccgcccgg taaagacctg gacgatttcg ctgacgaagc ctgtggccgg
     3541 caggaaaatg atatagactt cggggtgccc gaagaaccag aacaggtgct gccagagcag
     3601 cgcatcgcca tattcgtacg ggttgaagaa ctgcgtgccg accatgcggt cggccatcag
     3661 catggcgctc gtcagcgcga tcgagggcat cgagaagatc accatcagcg agaccaccag
     3721 catcgaccag gcaaacaccg gcatccgcgc tagcgtcatc cccggggctc gggccttgag
     3781 gatcgtcgca gtgatggaaa cggccacggc cagcgcggct acctcggtga aggtcaccat
     3841 ctgcgcccaa aaatccaccc gcttgcccgg cgagaactcg ggcccggcca gcggcacgta
     3901 ggaaaaccag cccgcatccg gcccggcgtt gagcacgaag gcgttccaca gcatcgatcc
     3961 gcccgccacg tacatccacc acgagaaggc gttgagccgc gggaaagcga ggttgcgcgt
     4021 tcccagcatc agcggcaaca ggtagatcgc aacggcatcc atcaccggca cggcgaagag
     4081 gaacatcatc gtcgtgccgt gcatcgtaaa gatctggttg tagcggtcag gcccgatcaa
     4141 agccttctgc ggcccggaaa gctgcagccg catcgccagc gccagcacgc cgccgagtgc
     4201 aaggaacacg aaggccgtga ccagatagcg ccgcccgatc gacttgtgat cgacgctcgc
     4261 cgcccagccc cagaaccccg gccggtcgcc ccaggtccgc gccagcttgt cgtggagggc
     4321 cgcgccggtc aggtccggcc cgtcgcgcag gtaagtccgc tcgctcactt caggctctcc
     4381 agataggcga ccaccgccgc gcgttcctcc ggcgaaagcg gcacctgcgg catctgcgtg
     4441 cccggcttca ctcgctgcgg atcggcgatc cagcggtcga ggctcgccgc cgtgttgggc
     4501 aggaccccgg aggcgatcgt cgtccggctg ccgaaatgag tgagatcggg cgcgacctgc
     4561 ccagaagccg gcgtccccgc gatattgtga caagaggcgc actgccgccc ggtgaacagc
     4621 gaatagccgg cgaattccgg cccgcctccc ggcaccggcg gcgtcgctag tccggcatta
     4681 taccacttcg cgaaatcccc gggcgcgcgc acgctcaccg gcagggccat ttgcgcatgc
     4741 tgcagcccgc agaactcggc gcactgggcg cggaactcgc cgggcttggg caccttcagc
     4801 accacgtcgc tggtccgccc cgggatcagg tcctgcttgc cggccaggtt ggggatccac
     4861 agcgaatgga ttacgtcggg cgaggagagc gaaaccttca ccgttgcccc cgccgggatc
     4921 accaattcgt tcgccgtccg cagctcgcgg ccggcgacgg gatcgtgata gcgcacctcc
     4981 caccaccact ggtgtccgat cacctcgacc gagagcgccg gcccggcccc ggcccgcgcg
     5041 atcgcccggt cggtgaacca cgtcgccacc gagagcccga acaggatcag cgcggaggtc
     5101 acggcaaaca gcacgaggcc cagccgccag cctgcttcct gctccggcct ggccgcgccc
     5161 tgcaccatcg cctgccgccg ccgcatcgcc aaggcgagga agaccatcac ccccagataa
     5221 atcggcacgg tcacccacag gaacaggtcg aacaggttcc cgatatgggc gctctccatg
     5281 ccctggccca tcgcgctcga ctgcggccca taaagcgccg agcacccgcc cagcgccatg
     5341 accagcagga ctgcgccgcg cctcatggac cggtcccctc gatcttagag ctctctttct
     5401 gcgccgcgtc ctgctccggc gtgaccgcgc gcgcgcgccg tcgcttttcg agcggaaggg
     5461 gctcgacgtt ggctggctcg tctgcccgcc cggacagtgc atcgcgccgc gtccgggccg
     5521 acatggagcg cacataggca gccagttccc aggcctggtc ctcggtgatc tttccgcgga
     5581 atgcgggcat tcccccaggc cggccgttga ggatggtata gaccacatcg gccatcgacg
     5641 acccgtagcg ccacttcgcg tccatcagtg gcggtcccat cccgccccct ccatgtgaat
     5701 ggcagccggc gcaattcatc cacacgaaca gccgagcgcc attggaaatc cgccaggcgt
     5761 tgccggagaa tttcgcgccg cggtggggcg aatccatcat cctgttggaa aagtcgcgcg
     5821 tctcgcgccc acaggcggcg agaagggtga gcgcggccag cggcagcaga ctaacgcggc
     5881 aaggcaaaga catagagcct ccctcccgcg tcggtccgct tgacaaggtc cttcatcgcg
     5941 ttgacgaagc cgagcgcagt ggtcggatcg cccggatcga gcgcaccgct gacaatcgcc
     6001 ccggaccagc cgccgacccc ggcgaagatc gcgacgtact gccgcccgtc cggccccttg
     6061 taggtcatcg gcatgccgat gatcccgctg accagcttgg tgcgccacag gagtcgtccg
     6121 gtgcgcgcgt cgatcgcctt gaaccagccg tccatggttc cgtagaacac cacgtcgccc
     6181 gccgtcgcga gggtgccgct ccacagcggc aggtcttccc ggatctccca caccttgcgg
     6241 cccgccaccg ggtcccaagc ggtgaacacg ccgcggtttc cgcccggtcc cggatacatc
     6301 cgcgcctccg tgccgaggta gggcgtgccg gcgatgtagt tggtgtccac gtcgccttcg
     6361 tccatgcaca ggttgttatg ggcgatgtag acgaggccgg tgcgcgggct gaacgagacc
     6421 gggttccagt ccttgccgcc gggcgcggcc gggcagatgt tgcgagtggt ccggccctcc
     6481 ttgggctcct tgtccgggtt catgatcggg cggccggagg tcagatccac gcccttcatc
     6541 gaattgatgt aggcaaaggg cttggcggaa agtacctgcc cgctcattcg gtcgatgatc
     6601 tggaagaagc cggaacgtgc tggccgggcg acgaccttgc gcagcccttg ccgcgtcggg
     6661 atgtcgagca gcaggctctc gttgatgtcg tcgtagtcga acaggtcgtg cgggctggtc
     6721 tggtagaacc accgcgcctg gccggtgcgc acgtcgcggg cgaagacgcc ggcagtccac
     6781 ttgttgtcgc cggggcgctg gtcggggttc cacggccccg gattgccggt gccatagaag
     6841 atcaagtgca gttccggatc gtaggacacc cagccccacg gattgccccc gccgatcttc
     6901 cacccttccg ggttccaggt gctcaccccc aggtccttgc cctggtcctg cttgtagaac
     6961 ggcctgaact ccggcccgat cagcacgtcc ttgtctggcc ccgtgctgta ggcccgccac
     7021 accagcttac ccgaggcggc gtcgagcgcc gccaggcttc cgcgcacgcc catctcgccg
     7081 cccgacgtgc ccaccagcac ccgcccttcg gcgagcagcg gcgccatggt gatcgtctcg
     7141 cctttggaaa tctcggcaag cttggccttc cacacctcgt ggcccgtcgc cgcgtccaac
     7201 gcgatggtct ggccgtccag cgtgttgaag tagagccgtc ccgctccgta aacggccccc
     7261 cggttgacca catcgcagca ggcaacgccc tgcgccgcct tctgcgactg cggatgatac
     7321 ttccacttca cgggtgcgcc cggcctggtc aagtcgagcg catagacgtc gttgggatag
     7381 ggtgtggtga catacatcgt atcgccgacc accagcgggc cggcctcatg cccacgcttc
     7441 aggccagttt cgaaggtgaa tacgggtttc aattgggcaa catttctggc gttgatgtcg
     7501 tctagcggtg agaaccgcga ggcggcgctg tcgccaagtt gccaggtcca ttgcccgtcc
     7561 ggcgagggct gagcccaaga ggccagccgc gcccgatcct gcggtgccgg aagagcacca
     7621 tagacgaccg gtctgtcgga gaatgtcgcg tgcggagccg gataggtggt cgccaggacc
     7681 gctgcgccga gcccgactag ggccgccagc aggatcatca gcacggaaac cggccagatc
     7741 gacttcatgc tgcgactgtc ccggcgtcgg gatcatgcct cacgcgacca catccccttg
     7801 ttaggcgtcg gggccggtgt tcgtcaggcc tgccgacttg ctgccgcata aacgacacaa
     7861 tattcgcccc gttccagacg ccggcactcc aagcagcttc ttgcaaaccg catttggtgg
     7921 aatctccacc cggttattgg acgaggggaa ccccatagct ggccagcaga ccagcaatct
     7981 cgctgcggcg ccgcgccagc accgcgtcga gactcgcgcg cagcttctga tcgtgcttct
     8041 gcactcccat cgagatgtca taggccattg gccattgcgc ctgatcgagc catggcgtta
     8101 ccggctcgat ccgcagcggc acggtcgagg tccgggcgta ccactccgcc acggggcccc
     8161 aagccagtgc cacgtcaatt tcaccgtcag cgacggcccg gacaatccgt gaaggcggat
     8221 tggcctcgcg atagtcgcca tagaccatgt agccgcgtac gttgtcgatg atttcgcgcc
     8281 gggcgatggc atgggcgggc ggagtgttca ttccgtcgtc gccgatcatc tgaacgccga
     8341 tccgcagttt gcgcagtcgc ggatcgtcca gcgtcaatcc ccccagccca gccgacctgc
     8401 gggtcacgaa gacgtaggtc gagcggtagt acggccgcgt tgtcgccatc atgtcgacag
     8461 tgctcgccac ccccggccac agatcgcact gtgcggcgtt caacgtattg cggacgtagc
     8521 cgcgccgctg tgcccaccac acgtactgca aggggcggtg cagatcggta gcgacaagct
     8581 ccgccagctt gttctcgaat cccgcgccgg cccgattgga gaacggcagg ttgttgggat
     8641 cggcgcacac ccggagcggc ttgcgcgaca aggcggagca tgagcccagg agcaaaagcg
     8701 agcccactgc gcccgcgagc agggtgatcc ggctcatttg cgcgccgcca ccacgcccca
     8761 cggccgcccg ccaacctgat cggtgcgcac cacgcgattg gagacaaggt ccaccgtggt
     8821 aagtgttccg gatagcccgc tagctgcata aagccgcttg ccatcgggcg aaatcgccag
     8881 gccccaggtg cgctcacccg tcgcgaagga ccggaccacc ttgcccgtgg ccgggtctat
     8941 ctcggcaacg cgattggtcc gtcccatcga gacgaacacg cgcctgccgt ccggcgttgc
     9001 gtgcatctcg acggcctgga cctgctcggc ctcggggaaa tccggcacca gatcgatcgt
     9061 cccggtgcgc tgcagcgtgg ccgcgtcaaa taccgcgatc agcccgcgct gttccgagga
     9121 gatccagact tgtcgcccgc cgccgacgaa gaggacgccg cgcggccggt tgccgaccgg
     9181 ggcgctcacg agcagccggc cgctggtcgc atcgatgaaa tgaactgtgc tcgatgcttc
     9241 ggccgttgcc accaccagcc ttccgtcggg gctgactccc atgccttcag gttcgggacc
     9301 gaccttgact tcggcgatga tcttgccgct gggcaggtcg agaaacgaga cggcgctgtg
     9361 atcctcgttg gcgatgtaga gccgcttgcc gtccgggctc acggcaaagc gctccgggtc
     9421 cgacccagag gaccacatgg ccaacctgcg ccgctctttc accgaccagg cctcgatccg
     9481 gttggctttg ctcgcggcca cgtagagcac ctggccatcg ggcgagagcg ccaagccgcg
     9541 ggggcgggcg ccggtcgcca aggcgccgac cgacttgccg ctggcgccgt cgagcacgtg
     9601 gaccacgccg ctcgcttcgt tcgagacgaa taccgtgtcg gtcgacgccg tctgcgattg
     9661 gcaggcgccg agcagcagag gcaacgcgag catgactgac gccagggccg ctcgaggctt
     9721 gatgagcata tccttgtccc cggcccgcgc ccgcctagcc cacggcgttt cctcgtgccg
     9781 acgagggaac aacgtcgaag cacttgcgat ccctgatggg cgggaagcag cgcgcctcag
     9841 ctgctgcgca agcgctgctc tgagcggtcg tcttcattct gtccgctgcg gtcgtggagc
     9901 aggactatct gggttgggaa cggcagatcc acgccgtggg cacgaaacgc ctggaggatc
     9961 gcctcgacca cccgcgagcg gatgtgcacc gcctcgctgc gcgccgaacg agtccaccac
    10021 cgggcgcgca gccgcacgtc gccatcgcga aactccaccg cgatgacttc cgggccgggc
    10081 tccgtctcga caccgtcgat gcgccggatc gtgtcggtga caaaggtcat cgccccagcc
    10141 agatcatcgt tgtacccaat cccgaaatcg gcttcgtcgc gacgggtcgc gaatgcggtg
    10201 ttgaccacga ccaccccggt gtagatgtcg gcgttcggta tgatcactcg acgtccatca
    10261 taggtgcgga tcagcgtggc gcggctctcg atgtgctcga ccgttccctc gtagtctttg
    10321 accgcaattt cgtctccccg gcgaaatggg cgcctggtca gcaggagcag gcccgcaaac
    10381 agattctgca ggatatcctt gaatgcgaag ccgatggcaa ccgagccaat ccccagcgtg
    10441 gccaggatat cggcgggttt tacgctgggg aagaggatgg cggcggcaac catcagcgcc
    10501 agtagcatga tcagcccgaa ggcgatggac gccagcagtt cgccaagatc gatccgctct
    10561 cgccggctca gcagcttgcg cacgccccac gctgccgtgc gggcaagccc ccacgccagg
    10621 agcaagaaca cggctgccgc agcgatgttc ggaacctgga ggaagaactc gttcgcccag
    10681 acatgcagct tggccaggat cagatcgccc ggctgcttga ttgggggcac gcgtaccgac
    10741 tgcatgagcg gctaacggga gagctttacg cgaggttcct gtagctcgcc ggacagattt
    10801 tggcgtgagc ggacattcaa gcgcgtgcga tggagtggcg tgaattggtc gccctcccaa
    10861 tcccaccata ccgggacgaa gcgtttcaga cgcgttaccc actcccgcat ggtggcgcgg
    10921 cgatggacgg aacgcaattt gcggcgctcc gttggcgaag catgaagcct gcttgcattg
    10981 gcatcgcctt ggctttggtc ggatgtgggt cgcagtcccg cagcgaatgc gaagccctgc
    11041 gaacgactga gagcgttctg aacgaactgg cctggtcagc ggaacaggcc gatgccggac
    11101 ggacgccgcg cacctatctc gaggcttttc agcggcaagg cgtgcagcag ctgaagcaat
    11161 cggcgcaaga actgcgcccg catgacccta ccgccgccgg gatcgtcgac ggggtggcag
    11221 gatatgcggg gacgccagat cggctgcggg ctgctgcccg ttcgctgcgc gccagagagg
    11281 caacccttga agccgcttga gctcacgctc ggcatcctca ccgccgtggg gggctttgtc
    11341 gatatcagcg aattggtgtt cgtctcgcag gccggagcgc ggttcggcta tgcactcatc
    11401 tgggtcatcg tgctggcgac ggtcgggatc atcgtctttg gcgagatgag cggacggatt
    11461 gccgccgtgt cgcggcagcc gatgttcacg atcattcgca aggagcttgg ccttaccctc
    11521 ggcggtgtga cgctgattgg cggtctcgta agcacgctcg tcacctgcgc agcggagatc
    11581 gggggcgtgg cgctggttct gcaattgctt accggcggca gctaccttct tctggcggcg
    11641 ctggccacga ccgtcttcgc cgcatcgatc tggctgctgc cgttcaagtg gatcgagcgc
    11701 agctacggcc tgctcgggct cgtcatgctc gtgtttgccg cggctctgct agccctcgat
    11761 ccaccgtgga gccacatcgc cgcagggctg gtcccacgcg tgccggccgg cctctctcag
    11821 caggatatgc tcgtttacgg ctacttcgtc gtggcgctga tcagcgcgat catgttcccc
    11881 tacgaactct atttttactc ctctggcgga gtcgaggacg cctggagcaa ggcagaccta
    11941 tcgaccaata gggtcacctc catcgtcggc atgagtctcg gcgcgctgat ggccatcgtg
    12001 ctgctctgcc ttgcagcgca ggaactcggg ccacgcggca tctcgcccga gcaggttggc
    12061 gccgtggtgc tccaacccgg gctcgccttc ggaaagctcg gcgttatcct gggtctgctg
    12121 ggaatcctga ccgctgtcgc gggagcggcg atcgagacct gcctcgccaa tgcctatgca
    12181 gtgagccagt ttttcgggtg gcggtggggg agacatcggc ccccgcagga tacgccgctg
    12241 ttcaccctcg cttggctggt cgtgttcgcg ctcgctctgg cgctggtctg caccggcgtc
    12301 gaagtcaccg atctggcaga ctacgcggtg gtgctttcga ttgtggtcct gccgctcagc
    12361 ttcctgccgc tgctgctcgc cggcaacgat cccgtactga tgcgcgatca ggtgaacggg
    12421 ccctggtcac gccggctcgg ctggctgttc tacggcctcg tcgtgctggc ggcggtcgcg
    12481 gccgtgccgc tttacctgtt gacgttggga ggcaagctgt gagagcggcc gatccccacc
    12541 acatcgccga cgccttcctc gacatgccac tggtcgatct ccacgaccac agttgcggag
    12601 tggttgacga catcgaactg gcggaaatcg aacctggcat ctgggagttg gcggcgattc
    12661 tggttggccc gggcgcctgg cggcgacggc gccccaggtg gctcaccgcc ttgctgccca
    12721 agctcaaggc cgtgcggatc gccaaggcgg acattgcgag cgctacacag gtggttcgct
    12781 tgatgcgccc ggccgcagag ctgggcctcg caacaacaga gcgccggctg ctggccctga
    12841 tgggcgggag gtagcgcggc tcagcacttg cgcaggcgct gctctgcgat cttgcaggat
    12901 gtcctttaac gccagaccga cggcgcccga gccagcttcg cttgatgctc gccgattggc
    12961 ggaaccttgc gttcgtgctg ggccgggcgc aaattactcc gatgggcgag ttcgaccatt
    13021 tggacgcgct ggacgctctc aaatacgcgg aggagagcga ggctgtccga cggttactgg
    13081 ctgatccggt gctcgacccg tcgcatagtg ccgcggtcgc ggccgatgca attgaactga
    13141 tctgcgcagc gcggcggatc ggtgaacgcc acggtgtggt tcagcgcttc cttcgggaat
    13201 actcgctcgc aagccgcgag gggcttgccc tcatgtgcct tgccgaagcg ctgctgcgaa
    13261 cgcccgacac ggcgacgcgt gaccggctga tcgccgagca agtcgcctcc gcggagtggg
    13321 tgaggcatct cggccattcg cggagcctat ttgtcaatgc ctcgacctgg gcgctgatgc
    13381 tcaccggcag gctgttcgaa ccggatgccg aagcgaggga gaacccaggc gctttcctcc
    13441 accggctcgc cgcgcgactg ggcgagccgg tcctgcgggc tgcggtggcc aaagccttgc
    13501 gcatcatggg cggacagttc gtactgggtg cggatatcgg gagcgcgctg gcgcgggcaa
    13561 agaaagagca cctgctctgt tcgttcgaca tgctcggcga gggtgctcgc accgtggcgg
    13621 acgccgaccg ctacttccgc cgctatgcca ctgcgatcca ggcggtggga gaggctgcgt
    13681 gcggctccgg cccagagctc gggcatggga tatcggtcaa gctctcagcg ctctggccgc
    13741 ggtgcgaagc tgcccacgag gcccgcgtat gggacgagct ctatccgcgg ctccggcacc
    13801 tggcccgggc ggcagctgat ttcggcctaa atctgacgat cgacgccgaa gaggcggatc
    13861 gcctggtact cggtctcaag ctgttcgacc gggtggcagg cgagacggaa ctcggagact
    13921 ggcgcggcct gggactggcg gtgcaggcct atcagcctcg ggccgtggcg gtgatcggcg
    13981 aagtcatcag gatcgcccgg cgccgtcacc gccggctgat agtgcggttg gtcaagggcg
    14041 cctattggga taccgagatc aagcgggcgc aggtctcagg catgtccggc tatccggtgt
    14101 ttacgaccaa gcccgcgacc gatgtcaatt atcttgcctg tgcgcgcgaa ctgcttgcag
    14161 caggcgaggc tatttacccg cagttcgcca cccacaatgc acatactctc gcggctgtcc
    14221 gccagctcgc agcgaaggtt gggcgcggga tagagttcca gcgcctgcac ggcatgggcg
    14281 aggcgctgta cgaagcggcg caggcgcgct ggggtgcgct gacggtacgg acctacgcgc
    14341 ccgtcggaga gcacgagcaa ctcttgccct atctcgttcg gcgcctgctc gagaacggcg
    14401 ccaacagctc cttcgtgcat cgcttggtcg acgaggagat cgcaccatcc gaggtggcgc
    14461 ctgaccccta tcgtcagctg gccgtaagcc cgggcccgaa ctcgaatatc cccctgccgc
    14521 gcctgctcta tggtgaaggc cgcctcaacc cgctgggccg cgacctgtcg gtcgcctccg
    14581 tccgggagaa cgctgcggcg gcggtggcaa gcctgcgaac tgagacgctc accgccgggc
    14641 cgatcattgc gggccggcag cttttggatg acggacg
//
LOCUS       DATNBV010000013         3243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_325841,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000013 DATNBV010000000
VERSION     DATNBV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325841"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..845
                     /locus_tag="VN627_00330"
     CDS             195..845
                     /locus_tag="VN627_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HXD01730.1"
                     /translation="MNDTTLDLAALLCSRLCHDMLSPVGALSNGLELLADEKDADMRG
                     RIVELLEQSAKNSADKLKFFRLAFGAAGGFGEMVPVDEARSVIDALVGNNGRIEARWA
                     VEGALPKPAVKTLLNFALIGIEALVRGGTLDLGAELREGANGAESEIVVRASGPRLAF
                     DPLVGKALQGTLGAGDLSSKTAPAAMLRQLANSLGGEVQSILTEDALVMGAVLPVR"
     gene            859..1563
                     /locus_tag="VN627_00335"
     CDS             859..1563
                     /locus_tag="VN627_00335"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXD01731.1"
                     /translation="MRRWLVHREVPSPNFNARKAPVSMVVLHYTGMRTAGDALERMCN
                     PAAEVSAHYMIEEDGTVTQLVDEEHRAWHAGKAYWRGETDVNSASVGIELVNPGHEFG
                     YRPFPEAQVEALLPLLADIVKRHNVPRANVVGHSDVAPARKQDPGELFPWEELARHHL
                     ALPIPALKMRLMYDNPGAFYLALERFGYDITDGRAAIRAFQRRFRPRRVDGELDGECG
                     ALLFELLLERDLGRAR"
     gene            1586..1995
                     /gene="rnpB"
                     /locus_tag="VN627_00340"
     ncRNA           1586..1995
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VN627_00340"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2046..>3243)
                     /locus_tag="VN627_00345"
     CDS             complement(2046..>3243)
                     /locus_tag="VN627_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HXD01732.1"
                     /translation="MWPHEMGILYKAAAAMSAGAGATFNYYADMAETFPFEEPRKAAG
                     EGLFGLVVREPVGVVGAIIPWNAPVSMISWKVAPALLAGCTVVIKASPEAPGAAYVFA
                     EVAEAIGMPAGVVNVVTADREVSELLVRSPLVDKISFTGSTAAGRRIASICGERIARV
                     TLELGGKSGAVILDDYDPELAAAKIAGPACALTGQICSSLTRIVVPRSRHDALVEALA
                     ARFKAVRIGNPFDDESEIGPIAVERQRTRIEGLIASGVAEGATLAAGGGRPSHLNRGF
                     YVEPTVFGNVENSMRIAQEEIFGPVLSIIPAEDEADAIAIANDTIYGLNNSVFTHDAD
                     RAYAVARQLRSGTVGHNSFRTDFGMGFGGFKQSGIGREGNIEGLYPYLEHKSVILEGV
                     PSHVTG"
BASE COUNT          550 a         1095 c         1075 g          523 t
ORIGIN      
        1 tgggcaatcg tcgcaaatgg agcgatcagt ctgtgggttg atgcgccgaa cggtttcacg
       61 gcgctttcct acgcggtgca ggagggttaa ggcggcgggg cagggccggc cggcgcttcc
      121 ttttggaggg gggctgtttt cgtgtcctga accgtgtcaa cttcgtcaac ttgttcagca
      181 ggccttccat ttccatgaac gataccacgc tcgatctggc cgccctgctg tgttcgcgtc
      241 tgtgccacga tatgctcagc ccggtcggcg ccctgtccaa cgggctcgaa ctgctggctg
      301 acgaaaagga cgccgacatg cgcggccgga tcgtggaact gctggagcag agcgccaaga
      361 acagtgccga caagctcaaa ttcttccgcc ttgccttcgg cgccgccggc ggcttcggcg
      421 aaatggtccc ggtcgatgag gcacgcagcg tgatcgatgc cctggtcggc aacaacggcc
      481 ggatcgaggc gcgctgggca gtcgagggcg cgctgcccaa gcccgcggtg aagaccctgc
      541 tgaacttcgc cctgatcggg atcgaggccc tggtgcgggg cggtacgctc gatctcggcg
      601 cggaactgcg cgaaggcgcc aatggcgcag agagcgagat cgtggtccgg gcctcgggcc
      661 cgcgcctcgc cttcgatccg ctggtcggca aggccctgca gggcacgctc ggcgcgggcg
      721 acctctccag caagaccgca cccgccgcca tgctgcgcca gctggccaac tcgctgggcg
      781 gcgaagtcca gtcgatcctg accgaagacg cgctggtcat gggcgcggtc cttccggtcc
      841 gttaggccgg tcgtggacct gcgccgctgg ctcgtccacc gcgaggttcc gtcgcccaat
      901 ttcaacgcgc gcaaggcgcc tgtctccatg gtcgtgctgc actacaccgg catgcgcacc
      961 gccggcgatg cactggagcg gatgtgcaat ccggcggcgg aagtttcggc gcactacatg
     1021 atcgaggaag acggcacggt cacccagctg gtcgatgagg agcaccgcgc ctggcatgcc
     1081 ggcaaggctt actggcgcgg ggaaaccgac gtgaactcgg ccagtgtcgg gatcgaactg
     1141 gtgaatcccg gtcacgaatt cggctaccgc ccgtttcccg aggcccaggt cgaggccctg
     1201 ctcccgctgc tcgccgacat cgtgaagcgc cacaacgtgc cgcgcgccaa tgtcgtcggc
     1261 cattccgacg tggcccccgc gcgcaagcag gatccgggcg agctgttccc atgggaggaa
     1321 ctcgcccgcc accatctggc cctgccgatc cccgcactca agatgcggct gatgtacgac
     1381 aacccgggcg ccttctacct cgccctcgag cgcttcggct atgacatcac cgacggccgc
     1441 gccgccatcc gcgccttcca gcgccgcttc cgcccccgcc gggtcgatgg cgagctcgac
     1501 ggcgaatgcg gtgccctgct gttcgagctg ctgctggaaa gggaccttgg acgggcgcgc
     1561 tagcccatgc taaggcgccc ccgcgccagg gggccgggca gccgcggcat ctcacgatga
     1621 cgaggaaagt ccgggctcca cgaaacgagg gtggcgggta acgcccgccg ggtccggccg
     1681 aaccttcggg ggaaaccggg cctagggaaa gtgccacaga gagtagaccg ccccgcaagg
     1741 ggtaagggtg aaagggtgcg gcaagagcgc accggggccc tggcaacagg gctcgcatgg
     1801 caaaccccac ccggagcaag accgaatagg ggctgcgtgc tcgcaagagc aggcgtgttt
     1861 cgcgccagca gcccgggttg gtcgcttgag cgtcgcagca atgcgccgcc tagaggaatg
     1921 gctgccccgg ccgatacggt gccctctggc ataccgtcgg ccggacagaa cccggcttac
     1981 aggctccctg gcgcatcacc ggaccggggg gggcggcgca cggccgcccc cccgccgtcc
     2041 atgcatcagc ctgtcacgtg gcttggcacg ccttcgagga ttaccgactt gtgctcgagg
     2101 tagggataga ggccctcgat attgccttcc cggccaatgc cggattgctt gaacccgccg
     2161 aagcccatac cgaaatcggt gcggaagctg ttgtggccga ccgtgcccga gcgcagctgg
     2221 cgggcaaccg cataggcccg gtcggcgtcg tgtgtgaaca ccgagttatt gaggccgtag
     2281 atcgtatcgt tggcaatcgc gatcgcatcc gcctcgtcct cggcggggat gatcgagagc
     2341 accggcccga agatttcctc ctgggcaatg cgcatggaat tctcgacatt gccgaacacc
     2401 gtgggctcca catagaaccc gcggttgagg tgcgagggtc ggcccccgcc cgcggccagg
     2461 gtggcgcctt cggccacgcc gctggcgatc aggccctcga tcctggtgcg ctggcgctcg
     2521 acggcaatgg ggccgatctc gctctcgtcg tcgaacggat tgccgatgcg cacggccttg
     2581 aaccgagccg caagcgcttc caccagcgcg tcatggcggc tgcggggcac cacgatccgg
     2641 gtgagcgatg agcagatctg acccgtcagc gcgcaagccg gtccggcgat cttcgcagct
     2701 gcgagttcgg gatcatagtc gtcaaggatc accgctcccg acttgccgcc gagctccagc
     2761 gtcacccggg cgatccgttc accgcaaatc gaggcgatgc gccggcccgc ggcggtcgat
     2821 ccggtgaagc tgatcttgtc caccaaaggc gagcgcacca atagctcaga cacttcccgg
     2881 tctgcggtga ccacgttgac gacaccggcc ggcattccga ttgcctcggc cacttcggca
     2941 aatacatagg cagcgcccgg cgcttcaggg gaagccttga tcaccaccgt gcagcccgcc
     3001 agcagggccg gtgcgacctt ccacgaaatc atgctcaccg gcgcgttcca tgggataatc
     3061 gcgccgacca cgccgacagg ctcgcgcacg accaggccga acaggccctc gccagcagcc
     3121 ttgcggggtt cctcgaaggg aaatgtctca gccatgtcgg catagtagtt gaacgtggcg
     3181 cccgccccgg ccgacatagc cgcggcggcc ttgtagagta tgcccatttc gtggggccac
     3241 atc
//
LOCUS       DATNBV010000014         2558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2565608,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000014 DATNBV010000000
VERSION     DATNBV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2565608"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VN627_00350"
     CDS             <1..639
                     /locus_tag="VN627_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HXD01733.1"
                     /translation="ARMEDVLDLYAEPPDPKRPVVCFDESPTQLIGEARQPIPAAPGQ
                     LERYDYEYRRNGTVNLFVFLDAHRPWRRVKVTDQRTARDFAECMRELVDLHYPQAQQV
                     RVVLDNLSTHSAGALYEAFPAPEAHRVLRRLEFHYTPKHASWLNMVEIEIGVLRGQCL
                     DRRIGARDCLVSEIAAWEQQRNAAGSRITWIFTTERARTKMARAYPDPAKES"
     gene            complement(654..1235)
                     /locus_tag="VN627_00355"
                     /pseudo
     CDS             complement(654..1235)
                     /locus_tag="VN627_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
     gene            1397..1693
                     /locus_tag="VN627_00360"
     CDS             1397..1693
                     /locus_tag="VN627_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXD01734.1"
                     /translation="MSEVTLFGFPHSSFVHIAQLVLMHKEVPYTFHDLEPDMGSPKHL
                     ALHPFDRVPILQHGDFVLYETSAIVAYLEELFPTPSLQPDTRQGRARMNQWISA"
     gene            1714..2088
                     /locus_tag="VN627_00365"
     CDS             1714..2088
                     /locus_tag="VN627_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HXD01735.1"
                     /translation="MIYHVSHERNVFPELGIPSDEAVVAHALPKIEVGLQVMERQLSH
                     AQGFLLGGEATLADYYLLPSTYSFGRTPEGKTMYASFPVVQAWRERMEALPTVRQLRQ
                     ATADLPPIEHARKWAVSHRPKY"
     gene            2234..2443
                     /locus_tag="VN627_00370"
     CDS             2234..2443
                     /locus_tag="VN627_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01736.1"
                     /translation="MTTRLFTTTTIDDRMLATCSAVSAHDVTPLEIQRWEDDGGAVLL
                     GVTPRHRLSSHPAAPGYTMPGSLAA"
BASE COUNT          455 a          902 c          757 g          444 t
ORIGIN      
        1 gcccgcatgg aggacgtcct ggacctctat gccgagcccc ccgatccaaa gcggccggtg
       61 gtatgcttcg acgagagccc gacccagctg atcggcgagg cgcgacagcc gatccccgcc
      121 gcgccgggcc agctggaacg ctatgattat gagtatcgcc gcaacggcac ggtcaacctg
      181 ttcgtctttc tcgatgccca ccggccctgg cgccgtgtga aggttaccga ccagcggaca
      241 gctcgcgact ttgctgagtg catgcgcgag ttggtcgacc tccactatcc gcaagcccag
      301 caggtccggg tcgtgctgga caatctatcg acgcattctg ccggcgcgct ctatgaagcc
      361 ttcccggcac ccgaggcgca ccgcgtattg cgccgcctgg agttccacta caccccaaag
      421 catgccagct ggctcaacat ggtcgagatc gagatcggcg tgctgcgcgg ccagtgcctc
      481 gaccgccgca tcggcgcgcg cgactgcctc gtctccgaaa ttgccgcctg ggaacagcag
      541 cgcaacgccg ccggctcccg catcacctgg atcttcacca ccgagcgcgc tcgcaccaaa
      601 atggcccgcg cttaccccga ccctgccaaa gagtcataat ccctgtgcag aggtactagc
      661 tccttcttct tggtgtcgac cgagatcgcc ggctcgcccg ccgccagcgc ctcgctcacc
      721 cgtgcattga ggtaaccgaa ctgcgcgtcg cggtcgggat ggctggttcc ttcgagggtc
      781 ttgcggttgg cctgcaagct gtagcccatc gcccgcagca gatcggccac aacgttatgg
      841 cagacgcgat ggcccagggc gccaagcccg gccgccaggt tgcgcaggct cttggccgtc
      901 cacaacagtg gcgccatcgg gtcgccgcga gccgtcggct caaccaggtc cttcaggtcg
      961 gaaagcaagc tcgggtcggt aacgaccagc ggccggcgcc caccgccttt gcggcgcacc
     1021 cgctcgggcc cggccatagg cgccgcatcg ccacgcagct ccgccagacc gcggtcgatc
     1081 gtgctgcgcg ccacgccggt gatccgcgac accgcggtta ccccaccatg ccccgccgcc
     1141 agcgcttcgg ccgccgcaaa cagccgccgg ccgcgctcgt tcaggatcgg atcaagcgcc
     1201 tcgtagcgca atctgatcgc cgcgtggtca atcacccacc gattctatga actccaactc
     1261 aacctgggaa tcccagatcg gcaacccaca ccgacaccga ttcggttatt gatcaacggc
     1321 ccctaaccgg gtcgagagct tgtggcggaa tgcattcgtt tcaactcccg gacccggcgg
     1381 cggcatggag aaaaccatgt ccgaagtcac cctgtttggc ttcccgcaca gcagcttcgt
     1441 gcatatcgcg cagctcgtgc tgatgcataa ggaggtgcca tacaccttcc atgaccttga
     1501 gcccgatatg ggctctccca aacacctcgc gctgcatcca ttcgatcggg tgccgatcct
     1561 gcagcacggc gatttcgtgc tttacgagac gagcgcgatc gtcgcctatc tcgaggagct
     1621 gttcccgacg ccttcgctgc agccggatac gcgacaaggc cgggcgcgga tgaaccagtg
     1681 gatcagcgcg taaattccta ctactacccg tacatgatct atcacgtctc acatgagcgg
     1741 aacgtgttcc ccgagctcgg tatcccgtcc gacgaggcgg tcgttgcgca tgcgctgccc
     1801 aagatcgagg tcggactgca ggtcatggaa cggcagctgt cgcatgctca gggcttcttg
     1861 ctcggcggcg aagcgacgct tgccgattat tacctgctgc cgagcaccta ttcgttcggg
     1921 cgcaccccgg agggcaagac gatgtacgcg agcttcccgg tcgtgcaggc ctggcgcgaa
     1981 cggatggagg ccttgccgac tgtgcggcag ctccgccagg caaccgcgga tctgccgccg
     2041 atcgagcacg ccaggaaatg ggccgtctcg caccggccca aatactgaaa cgacggcggg
     2101 gatagccgcc gcatgttgac gtgacgctac ggggatcgca ggctccccgt cagaggcacc
     2161 gcctccaagt tctgcgcttt tcaaacctcg gccgcggtgc ctgcccggcc ggaacaggaa
     2221 cggagaagcg cacgtgacaa ctcgcctatt caccacgacg accatcgatg accggatgct
     2281 ggcaacctgc tcggctgtgt cggcccatga cgtgaccccg ctcgaaattc aacgctggga
     2341 ggatgacggc ggtgccgtgc ttctcggcgt cacgccgcgg catcggctgt catcgcaccc
     2401 cgccgctccc ggttacacga tgccgggctc cttggcggcc tagtgctacc ggttgattgg
     2461 tatggggacg ccaggctccc cgtcagaggc aacccgcctc caagttctgc gcccttcagc
     2521 caacggatct gaacctctcc gctgcgcgac atgcgcca
//
LOCUS       DATNBV010000015         3444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1285864,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000015 DATNBV010000000
VERSION     DATNBV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285864"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VN627_00375"
     CDS             <1..1116
                     /locus_tag="VN627_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXD01737.1"
                     /translation="TFLAGGTGHEPDVVSTIDDLACVIFTSGSTGRPKGCILDSRGLA
                     LKAEYHCTDLGITSEDRVLVVMPLFHVGAQSMASAGQWNGAEVHLHRGFDAGEFIRTV
                     ERERITIAHLAPTMVQMIVEHPFAATADLSSIRGILYSAAAMPDPVLRRALELFGPVL
                     HHAYGQTEGIVSRLPRHQHRPDGTERERRRLLSVGQPYPGVTVRLIDDEGQDVTDSGT
                     GEIVYRGGATFRGYWNDHVATMATLRDGWVHSGDIGKFDEDGFLYLVDRKKDMIVTGG
                     ENVASREVEEALLAHPDVAEVAVIGVPHPRWVETVHAVVVRTPEGKVDADGLIAHCRT
                     LLASYKKPTGVTFVEHLPTLANGKIDKVTLRRDIAGG"
     gene            complement(1113..1883)
                     /locus_tag="VN627_00380"
     CDS             complement(1113..1883)
                     /locus_tag="VN627_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXD01738.1"
                     /translation="MGITVAVDGAVAVVTIEQPSRRNAMTLEMRAQAQREFERLCDDP
                     EVRAIVLTGAGEHFSAGADVSDMGQSGVGGSILKMRYIHRMVRAVNRTEKPVIAAVRG
                     VCIGMSWSLALACDFVIAAEDARFQFAFRHLGLAPDGGAVFQLTRLVGLQRAKEIIYS
                     GRFVSGSEARSLGLALEALPADEVLSRALAFAGELALAPTTAIAMAKRQFDAAPAQTL
                     DQALDFEGVMQAAMINTDDFREGAAAFREKRKPSYVGR"
     gene            complement(1968..>3444)
                     /locus_tag="VN627_00385"
     CDS             complement(1968..>3444)
                     /locus_tag="VN627_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HXD01739.1"
                     /translation="RTETADHATLHLAPRPESDWALVLAMLRTIFDNGWERLPERGAI
                     AGLAELRALAASADPTELAAICDVPLADIAEAARRFALAPRGYAMAATGPALGRNGVI
                     SHWLVLALNVVTGRIDVPGGRYLPAWPLNMTAYRQEFTPPMPVPSRVRGIPSVLGWHS
                     IAELADEIETPGSGQVRALFITGGNPVSSSAHGERLARALGGLDLLVALDMFRRESHR
                     HADWLIPGQHFLERDELLMAWSQAERPFVQAARAALPSPEGIRPEWTFFRDLADALGL
                     KLFGGQMEPHPDALAEALFAYGGQTTLAETRAAEHGLEFGERSMGHLWDYLAAQGTTV
                     ALCPEEFVAELHNALAQSRTTGGASFQIISRRRNNMMNGWLAETSGSVADDPTASSVE
                     INPGDAARIGLADGAQARIASRAGELVATVAVSDAVRPGTLVIASGWGAALYDPVSGA
                     EVFRKGIERNKLVADDDLGPLSGVPRLNGTPVTISAVQGGA"
BASE COUNT          546 a         1222 c         1158 g          518 t
ORIGIN      
        1 accttccttg ctggcggaac gggtcatgag cccgacgtcg tgtccaccat cgacgatctc
       61 gcctgcgtaa tcttcaccag cggcagcacc ggccgcccca agggctgcat cctcgattcg
      121 cgcgggctcg cgctgaaggc cgaataccat tgcaccgacc tcggcatcac gagcgaggac
      181 cgcgtgctgg tggtcatgcc gctgttccac gtcggcgccc agtccatggc cagcgccggc
      241 cagtggaacg gtgccgaggt ccatttgcat cgcgggttcg atgccggtga attcatccgc
      301 acggtcgaac gcgagcgcat aaccatcgcg cacctggcgc cgaccatggt gcagatgatc
      361 gtcgagcatc cgttcgccgc cacggcagac ctgtcctcga tccgcggcat cctctattcg
      421 gccgcggcca tgcccgatcc ggtcctgcgc cgcgcgctcg aactgttcgg cccggtgctc
      481 caccacgcct atggccagac cgaaggcatc gtctcgcgcc tgccgcgcca ccagcaccgg
      541 cccgatggca ccgagcgcga gcggcggcgc ctgctctcgg tcggccagcc gtacccaggc
      601 gtcactgtcc gcctgatcga cgatgaagga caggacgtaa cggacagcgg aaccggcgaa
      661 atcgtctacc gcggcggcgc caccttccgg gggtactgga acgaccatgt cgccacaatg
      721 gccaccctgc gcgacggctg ggtccactcg ggcgacatcg ggaagttcga cgaggatggc
      781 ttcctctatc tggttgaccg caagaaggac atgattgtca ccggcggcga aaacgtcgcc
      841 tcgcgcgagg tcgaagaggc cctgctcgcc catccggacg tggccgaagt ggcggtcatc
      901 ggcgtgccgc acccgcgctg ggtcgaaacg gtccacgccg tcgtcgtccg cacgccggag
      961 ggcaaggtcg atgcggatgg gctgatcgcc cactgccgta ccctgttggc ttcctacaag
     1021 aaaccgactg gggtgacgtt cgtggaacac ctgccgacgc tggccaacgg caagatcgac
     1081 aaggtcaccc tgcgccgcga catcgccggg ggctagcgcc ccacgtagct cggcttgcgc
     1141 ttctcgcgga aggcggcggc tccctcgcgg aagtcgtcgg tattgatcat cgccgcctgc
     1201 atgacgccct cgaagtccag cgcctggtcc agcgtctgcg ccggggccgc gtcgaactgg
     1261 cgcttggcca tggcaatggc cgtggtcggt gcaagggcca gttcgccggc aaaggcgagg
     1321 gcacgggaca gcacctcgtc ggcgggcagc gcctcgagcg cgaggccaag cgaacgggcc
     1381 tcgctgccgc tgacgaaccg cccggaatag atgatctcct tcgcccgctg caggccgacc
     1441 aggcgggtca gctggaacac ggccccgccg tcgggcgcaa ggccaaggtg gcggaaggcg
     1501 aactggaagc gtgcatcctc ggccgcgatc acgaagtcgc aggccagcgc cagcgaccac
     1561 gacatgccga tgcagacccc gcgcacggcg gcgatcaccg gcttctcggt ccggttcacc
     1621 gcgcgcacca tgcggtgaat gtagcgcatc ttgaggatcg atccgcccac tccgctctgg
     1681 cccatgtcac tgacatcggc cccggcggaa aagtgctctc cggccccggt cagcacgatg
     1741 gcgcggacct cgggatcgtc gcagaggcgc tcgaactcgc gctgcgcctg cgccctcatc
     1801 tccagtgtca tggcgttgcg ccgcgagggc tgctcgatcg tcactactgc gactgcgccg
     1861 tccacggcga cggtaattcc catagcttgc ctgctcctct tcctcgcccc gattatcggc
     1921 gcccgacgca atcggcaaag tgcccgcgcg agtcagcaca tcctcgttca ggcaccgccc
     1981 tgaacggctg agatggtcac cggtgtaccg ttcagccggg gcacgccgct aagcgggccg
     2041 aggtcgtcgt cggccaccag cttgttgcgc tcgatgccct tgcggaacac ttcggctccg
     2101 ctgaccggat cgtacagcgc cgcgccccag ccgctggcga tgacgagcgt gccggggcgc
     2161 accgcatccg atacggctac cgtcgcgacc agttcgcccg cccggctggc gatccgcgcc
     2221 tgcgccccgt cagccagccc gattcgcgcg gcgtcgcccg ggtttatctc caccgaactc
     2281 gccgtaggat cgtcggccac gctgccggaa gtttcggcca gccagccgtt catcatgttg
     2341 ttgcgacgcc gcgaaatgat ctggaagctg gctccgcctg tcgttcggga ctgtgccagc
     2401 gcgttatgca gctcggccac gaattcctcc gggcacagcg ccacggtcgt cccctgggct
     2461 gcgagatagt cccacaggtg ccccatcgac cgttcgccga attcgaggcc gtgctcggcc
     2521 gcgcgcgtct cggcaagggt ggtctgcccg ccataggcaa acagcgcctc ggcgagggcg
     2581 tcggggtgcg gctccatctg gccgccgaac agcttgagcc ccaaggcatc ggcaaggtcg
     2641 cggaagaagg tccactcggg gcggatgccc tcaggcgagg gcagggcagc gcgggcagcc
     2701 tggacgaacg ggcgctcggc ctgggaccag gccatcagca gttcgtcgcg ttcgaggaag
     2761 tgctggcccg ggatcagcca gtcggcgtgg cggtggctct cgcggcggaa catgtccagc
     2821 gccaccagca ggtccagccc gcccagggcc ctggccagcc gctcgccatg ggccgaggac
     2881 gacaccgggt tgcctccggt gatgaacagg gcccgcacct ggccggagcc gggtgtctca
     2941 atctcgtcgg ccagttcggc gatcgagtgc cagccgagga ccgacgggat gccgcgcacc
     3001 cggctgggca ccggcatggg cggcgtgaac tcctggcgat aggcggtcat gttgagcggc
     3061 cacgccggga gatagcggcc gcccggaacg tcgatccggc cggtcaccac gttcagcgcc
     3121 aggaccagcc agtgcgaaat cacaccgttg cgccccagcg ccggccccgt cgccgccatg
     3181 gcatagccgc gcggggccag ggcgaaccgg cgggcggctt cggcgatgtc ggccaggggc
     3241 acgtcgcaga tggcggcaag ctcggtggga tcggcgctcg cggcgagagc ccgcaactcc
     3301 gccagcccgg caatcgcccc gcgctcgggc aggcgctccc agccattgtc gaagatcgtg
     3361 cgcagcatcg caagtaccag ggcccagtcg gactcggggc ggggcgcgag gtggagcgtc
     3421 gcatggtcgg ccgtctcggt gcgc
//
LOCUS       DATNBV010000016         3374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2246244,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000016 DATNBV010000000
VERSION     DATNBV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2246244"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /gene="glmU"
                     /locus_tag="VN627_00390"
     CDS             <1..1242
                     /gene="glmU"
                     /locus_tag="VN627_00390"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HXD01740.1"
                     /translation="GGLGASRVAVVVGPGMGSVVEAARGGLGSAVGVGVEQAERLGTG
                     HAVLMAKAALRGCSGPVVVVFGDTPLIRGETLRRLAEGIASGHAVMVAGMRPADPGAY
                     GRLVVGADGTLERIVEAKDANDAERAIGLCNAGAMALDGTVMWDLLERIAPNNAKGEY
                     YLTDVVALARARGLRCGVIEAAFEEAVGVNSRVELAEVEALFQARKRRDAMLGGATLT
                     DPASVWFSHDTVLGRDVVVGPNVVFGPGVTVGDHVAIKGFCHLEGCTIASGAQVGPFA
                     RLRPGAAIGADAHIGNFVEIKAALVGQGAKVNHLSYVGDAEVGAKANVGAGTITCNYD
                     GFTKAKTKIGAGAFIGSNTALVAPVSVGDGAIIGAGSVIVADVAPDALAVARGKQEEK
                     PGWAARFRALKAAAKLKSKRG"
     gene            1245..3068
                     /gene="glmS"
                     /locus_tag="VN627_00395"
     CDS             1245..3068
                     /gene="glmS"
                     /locus_tag="VN627_00395"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXD01741.1"
                     /translation="MCGIIGIIGKDAAAPHLIDALKRLEYRGYDSAGVATVVNGAIER
                     RRAEGKIVNLEALLQKQALAGTTGIGHTRWATHGVPNETNAHPHATKRVAVVHNGIIE
                     NFAALKAELEGQGCRFETQTDTEVVAHLLTVLLDQGKTPEQAMAAALKRFEGAFALAV
                     LVAGRSDLLLAARRGSPLAVGYGNGEMYVGSDALALAPFTRRISYLEDGDWAAVTAQG
                     ARIFDEKDHEVQRPIRETALTGALIGKGNHRHFMHKEIHEQPAIVGDTLRAYLNPATL
                     QVGLPALPFDFKTLPKLTIVACGTSSYAGMVAKYWFEGLARLPVEVDIASEFRYRDPP
                     FVAGGASLFISQSGETIDTLSALRHAKSQGQHVAAIVNVPESSIAREADVVFPTHAGP
                     EIGVASTKAFTAQLTVLACLALAAAKARGTLDAKHAGELAVALMEAPARLAEVLAHDD
                     TIRELAHSIAEARDVLYLGRGASYPVALEGALKLKEISYIHAEGYPAGEMKHGPIALI
                     DEHVPVIFVAPADGAFDKTASNAQEVAARGGKVILLSDPAGIAKLKSLAKATVEMPKT
                     HPFVAPILYAVPVQLLAYHTAVLKGTDVDQPRNLAKSVTVE"
BASE COUNT          556 a         1043 c         1258 g          517 t
ORIGIN      
        1 ggggggctgg gggcgagccg ggtggcggtg gtggtgggtc ccgggatggg ctcggtggtg
       61 gaggcggcgc ggggcgggct ggggtctgct gtgggggtgg gggttgagca ggcggagcgg
      121 ctggggacgg ggcacgcggt gctgatggcg aaggcggccc tgcgcgggtg ttcgggtcct
      181 gtggtggtgg tgttcgggga cacgccgctg atccgcggcg agacgttgcg gcggttggcc
      241 gaggggattg cttcggggca tgcggtgatg gtggcgggga tgcggcccgc ggatccgggg
      301 gcgtatgggc ggctggtggt gggtgcggac gggacgctgg agcggatcgt cgaggcgaag
      361 gacgcgaacg atgctgagcg cgcgatcggc ttgtgcaacg ccggtgcgat ggcgttggac
      421 gggacggtca tgtgggatct gctggagcgg atcgcaccga acaacgccaa gggcgagtac
      481 tacctgaccg acgtggtggc tttggcgcgg gcgcggggcc tgcgctgcgg ggtgatcgag
      541 gcggcgttcg aggaagcggt gggggtcaac tcgcgcgtcg agctggcgga ggtggaggcg
      601 ctgttccagg ccaggaagcg gcgtgacgcg atgctgggcg gggcgacctt gacggatccg
      661 gcgagcgtgt ggttcagcca cgacacggtg ctggggcgcg atgtcgtggt gggcccgaac
      721 gtggtgttcg gccccggtgt gacggtgggc gaccatgtcg cgatcaaggg cttctgccat
      781 ctcgagggct gcacgatcgc gagcggggcg caggtcggtc cgttcgcgcg attgcggccg
      841 ggggcggcga tcggcgccga tgcgcatatc ggcaacttcg tggagatcaa ggcggcgctg
      901 gtcggccagg gcgccaaggt gaaccacctg agctatgtcg gcgatgccga ggtgggcgcg
      961 aaggcgaatg tgggcgccgg gacgatcacc tgcaactatg acggcttcac caaggcgaag
     1021 acgaagatcg gggcgggtgc gttcatcggt tcgaacacgg cgctggtggc gccggtctct
     1081 gtcggcgatg gcgccatcat cggcgccggc agcgtgatcg tggcggacgt ggccccggat
     1141 gcgctggcgg tggcgcgcgg caagcaggaa gagaagccgg gctgggccgc gcgcttccgg
     1201 gcgctgaagg cggccgccaa actcaagagc aagcgaggct agcgatgtgc gggatcatcg
     1261 gcatcatcgg caaggacgcg gcggcaccgc atctgatcga cgcgctgaag cggctggaat
     1321 accgcggcta cgattcggcg ggcgttgcga cggtggtgaa cggcgccatc gagcgccgcc
     1381 gcgccgaggg caagatcgtc aatctggaag cgctgctgca gaagcaggcg ctggcgggca
     1441 cgaccgggat cggccacacg cgctgggcga cgcatggcgt gcccaacgag accaacgcgc
     1501 acccgcacgc cacgaaacgc gtggcggtgg tgcataacgg catcatcgag aacttcgcgg
     1561 cattgaaggc ggagctggaa ggccagggct gccgcttcga gacgcagacc gataccgaag
     1621 tggtggcgca tctgctgacc gtgctgctcg accagggcaa gacacccgag caggcgatgg
     1681 cggcagccct caagcgcttc gagggggcgt tcgcgctggc cgtcctggtg gcgggccgca
     1741 gcgacctgct gctggcggcg cggcgcggct cgccgctggc ggtgggctat ggcaacggcg
     1801 agatgtatgt cggctcggat gcgctggcgc tagcgccgtt cacgcggcgg atcagctatc
     1861 tcgaggacgg cgactgggcg gcggtgaccg cacagggcgc gcgcatcttc gacgagaagg
     1921 accatgaggt gcagcggccg atccgcgaga cggcgctgac gggggcgctg atcggcaagg
     1981 gcaaccaccg ccacttcatg cacaaggaga tccacgagca gcccgccatc gtcggcgata
     2041 ccctgcgcgc ctatctcaac cccgcgacct tgcaggtggg gctgcccgcc ctgccgttcg
     2101 acttcaagac cctgcccaag ctgaccatcg tcgcctgcgg cacctcctcc tatgccggca
     2161 tggtggcgaa gtactggttc gagggtctcg ccaggctccc cgtcgaggtc gatatcgcct
     2221 cggagttccg ctaccgcgat ccgcccttcg ttgcgggcgg cgccagcctg ttcatctcgc
     2281 agtcgggcga gacgatcgac accttgtcag ccctgcgcca cgccaagagc cagggccagc
     2341 acgtggcggc gatcgtcaac gtgccggaga gctcgatcgc gcgcgaggcc gacgtggtgt
     2401 tcccgaccca tgcgggtccg gagatcggcg tggcctcgac caaagccttc accgcccagc
     2461 tcaccgtgct cgcctgtctc gccctcgcgg cggcgaaagc gcgagggacg ctggatgcca
     2521 agcatgcggg cgaactcgcg gtggcgctga tggaagcgcc ggcgcgcctc gccgaggtgc
     2581 tggcgcatga cgacacgatc agggagctcg cccactcgat cgccgaagcg cgcgacgtgc
     2641 tctatctcgg ccgcggcgcc tcctacccgg tcgccctcga gggcgccctc aagctcaagg
     2701 agatctccta catccacgcc gagggctatc ccgccggcga gatgaagcac ggccccatcg
     2761 ccctcatcga cgagcacgtc cccgtcatct tcgtcgcccc cgccgacggc gccttcgaca
     2821 agaccgcctc caacgcccag gaagtggcgg cccgcggcgg caaggtcatc ctgctctccg
     2881 accccgccgg catcgccaag ctcaaatccc tcgccaaggc caccgtcgag atgcccaaaa
     2941 cccacccctt cgtcgccccc atcctctacg ccgtccccgt ccagctcctc gcctaccaca
     3001 ccgccgtcct taaaggcacc gacgtcgacc agcccagaaa cctcgccaaa tccgtcaccg
     3061 tcgagtgacg agtgacccct cgggctgtaa agaaacgcaa cggggtgtaa agccgcgcga
     3121 ctaaaagggc gtgtggttaa ctcataagta attgttttcg catgaaactt gcggctggag
     3181 cggtagcagg agccgctacc ggcatggcat ggcaattgct tgttttgcag cgcaagttcg
     3241 atctctgcga acaggttaac actgcagccg cgaccggtgt tcgtaagggg tgataagtct
     3301 ctgacaagca agggttgtcc tgaaaccggc gggacagaat gacggaagac gctggatgag
     3361 ctatactttc ggac
//
LOCUS       DATNBV010000017         4330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2885533,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000017 DATNBV010000000
VERSION     DATNBV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2885533"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VN627_00400"
     CDS             <1..330
                     /locus_tag="VN627_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01742.1"
                     /translation="YNALVEDGRKGAATAYVDGAIAEGRIGLKPVRDEYIALHMADAD
                     RAVKLISAMPKIGGGSAAIADGDPAGRAAAQEFGAEDRLVMSLFGIDEDEYRDELKAR
                     GLLKEVL"
     gene            331..741
                     /locus_tag="VN627_00405"
     CDS             331..741
                     /locus_tag="VN627_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01743.1"
                     /translation="MAALAAARSTPEKVGAHRAIPLAANAKPFQGGMVQINAAGYGVA
                     ASATAANVTVGVAVETVDNTGGANGAVSVKTKRGVYRMANSSAGDLIARSEIGKTVYV
                     VDDQTVAKTDNTGARPAAGKCFDVDAQGVWVEFL"
     gene            754..1653
                     /locus_tag="VN627_00410"
     CDS             754..1653
                     /locus_tag="VN627_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HXD01744.1"
                     /translation="MIISSDNLNKLRIGFNKAMQRGLGQATAMSSRIATRVPATQRQQ
                     SYGWLNKLPNVREWIGARVVQNLSESDYTIKEKPWELTIGVDRDDIETDNLGQYDMLF
                     AQMGHSTSGFVEQLVFNQLLAGFSTNCFDGQFYFDTDHPILDANGVATTFANTDGGAG
                     TPWFLVDVSQAILPVILQVRRDFGEIVAKDKVTDDNVFDLNEFRYGVDARMNVGFSFP
                     QLAWGSKQTLDAAHYETAKAAVGSMKGDHGRPLGLGTKLVLMVPPSLEGAGRKILASQ
                     LVNGGESNPWAGSAELVVVPWLA"
     gene            1666..2025
                     /locus_tag="VN627_00415"
     CDS             1666..2025
                     /locus_tag="VN627_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01745.1"
                     /translation="MANRPKRAAGAKPAADAPAKVVAPAGVASAEQAPPEQGEQARSG
                     PADPLGPEGDLDAATIAVRSVGAKGRWRCGRHFTQEPTKIAGADLTDDEIGRLLADPE
                     LIVGLVKDAADPAADED"
     gene            2041..2469
                     /locus_tag="VN627_00420"
     CDS             2041..2469
                     /locus_tag="VN627_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HXD01746.1"
                     /translation="MNYASQSALVDRFGERLLLDLTDRADPPAGAVDSAIVDRALADT
                     DAVIDGYLAGRYILPLATTPPLLADLALQIAIYKLHVFAPDQKIADDYKAALAMLGKI
                     ADGTVRLPAAGVEPASSGSAGVETIDRERPLTPENMTGFV"
     gene            2483..2920
                     /locus_tag="VN627_00425"
     CDS             2483..2920
                     /locus_tag="VN627_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01747.1"
                     /translation="MIRIDDVRARIEGKVPALAGQIGNAGEFSKLIEAGQMQAARKAY
                     VLPGALAGGARQAISGMFTQALEETVLVVLSVRVASDPTGARALDEISPLVRDTVRAV
                     CGWGPEDAPGVFALARGELVGAAQGMLIYQLDFTLDDQLRITP"
     gene            2917..3087
                     /locus_tag="VN627_00430"
     CDS             2917..3087
                     /locus_tag="VN627_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01748.1"
                     /translation="MSRKTADAEAPAADIEAPAAEPVTAPEQLPQTGGCFIRNPDGTL
                     TRDPAFHGEQEG"
     gene            3091..4023
                     /locus_tag="VN627_00435"
     CDS             3091..4023
                     /locus_tag="VN627_00435"
                     /inference="COORDINATES: protein motif:HMM:NF039819.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HXD01749.1"
                     /translation="MPKFWRSKTLLAKAEATYGVDPAPTGAANAILAMNVTFTPQEGE
                     VVKRAVERPYFGAQPFVAQSFRASLSFEVDLVGSGAAGTAPAWGPLLRACAVAQTVTA
                     GVKVEYTPITDSQESVAMYFDVDGIKHVMLGAKGNAVIRAGVNGNPVLAFNLMSLFTA
                     PVDAAKPSPDYTAWPDPQAASKANTPTYTIGGAAFVARSYELDLGNQVEPRMLIGYEG
                     ILITGREESLKMTVEAVAAATFNPPAVVAAGTKLATSLLHGTVVGRKVQVDTPAAQLL
                     PIGQLAEQQGVVEWTLPFVPLPVTGNDQWKITLT"
     gene            4103..>4330
                     /locus_tag="VN627_00440"
     CDS             4103..>4330
                     /locus_tag="VN627_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01750.1"
                     /translation="MFKITTNPTFTHSVHVMVPVDGGHKEQTFKATFAVMDVEELERV
                     QDEEGQRGVLRKVIKGFDELIGDDDKPVPFSD"
BASE COUNT          753 a         1442 c         1489 g          646 t
ORIGIN      
        1 tacaatgccc tggtcgaaga tggccgcaag ggtgctgcga cggcttacgt cgacggcgcg
       61 atcgccgaag gccggatcgg cctgaagccg gtgcgagacg aatacatcgc gctgcacatg
      121 gccgatgccg accgcgcagt gaagctgatc tcggccatgc ccaagatcgg tggcggcagc
      181 gcggcgatcg ccgacggcga tccggccggc cgggccgctg cccaggaatt cggagccgaa
      241 gaccgactgg tgatgtcgct cttcgggatc gatgaagacg agtaccgcga cgaactgaag
      301 gcgcgcgggc tcctgaagga ggtcctctaa atggctgctc tcgctgctgc gcgcagcacc
      361 cctgaaaagg tcggtgccca ccgcgccatt ccgctcgccg ccaacgccaa gccgttccag
      421 ggcggcatgg tccagatcaa cgccgccggg tacggcgtgg ccgcttcggc gactgccgcc
      481 aacgtcacgg tcggggtcgc ggtggaaacc gtcgacaaca ccggcggcgc caacggcgcg
      541 gtctcggtca agaccaagcg cggggtctac cgcatggcca attcctcggc cggcgacctg
      601 atcgcccgct ccgagatcgg caagaccgtc tacgtcgtcg acgaccagac ggtggccaag
      661 accgacaaca ccggcgcgcg ccccgcagcc ggcaagtgct tcgacgtgga cgcccagggc
      721 gtctgggtcg agttcctctg aggaaggccc gtcatgatca tttcgtccga caatctgaac
      781 aagctgcgca tcggcttcaa caaggcgatg cagcgcggcc tcggccaggc caccgcgatg
      841 tcctcgcgca tcgccacccg cgtacccgcg acgcaaaggc agcagtccta tggctggctc
      901 aacaagctgc cgaacgtgcg cgagtggatc ggtgcccgcg tcgtccagaa cctctccgaa
      961 agcgattaca cgatcaagga gaagccctgg gaactgacca tcggtgtcga tcgcgacgac
     1021 atcgagaccg acaacctcgg ccagtacgac atgctgttcg cccagatggg ccattcgacc
     1081 agcgggttcg tcgagcagct ggtgttcaac cagctcctcg ccgggttctc gaccaactgc
     1141 ttcgacgggc agttctactt cgacacggac catccgatcc tcgacgcgaa cggcgtggcg
     1201 accaccttcg ccaataccga tggcggcgcg ggcaccccgt ggttcctggt agacgtgagc
     1261 caggcgatcc tgccggtcat cctgcaggtc cgccgcgact tcggcgagat cgtggcgaag
     1321 gacaaggtca ccgacgacaa cgtcttcgac ctcaacgaat tccgctatgg cgtggatgcg
     1381 cggatgaacg tcggcttctc gttcccgcag ctggcctggg gatcgaagca gacgctcgac
     1441 gcggcgcact acgaaaccgc caaggccgcg gtcggcagca tgaagggcga ccatggccgc
     1501 ccgctcggcc tcggcaccaa gctggtactg atggtgccgc cctcgctcga aggcgcgggc
     1561 cgcaagatcc tcgcctcgca gctggtcaac ggcggcgaaa gcaacccctg ggcgggttcg
     1621 gccgaactgg tcgtcgtgcc ctggctcgcc tgaggagact gagcgatggc taaccgtccg
     1681 aagagggctg ccggcgccaa gccggcagcc gacgccccgg ccaaggtggt cgcaccggcc
     1741 ggggtggctt cggccgaaca ggctccgccc gagcaaggcg agcaggctcg gtccgggccg
     1801 gctgatccgc tcgggcctga aggcgatctc gacgcggcca cgatcgcggt gcggtcggtg
     1861 ggagccaagg ggcgctggcg gtgcggccgg cacttcaccc aggagccgac gaagattgcg
     1921 ggcgccgacc tgaccgacga cgaaatcggc cggctgctcg ccgatcccga actgatcgtc
     1981 ggcctggtca aggatgcagc cgatccggct gcggacgaag actaaggggg gaagcggccg
     2041 gtgaactatg ccagccagtc tgcactggtc gaccggttcg gggagcgcct gctcctcgac
     2101 ctgaccgatc gcgccgatcc gcctgcgggg gcggtggaca gcgcgatcgt cgaccgggcg
     2161 ctggccgaca ccgacgcggt gatcgatggc taccttgccg gccgctatat cctgccgctc
     2221 gcgaccacgc cgccgctgct ggcggacctg gcgctgcaga tcgcgatcta caagctgcac
     2281 gtcttcgcgc ccgaccagaa gatcgccgac gattacaagg cggcgctggc gatgctcggc
     2341 aagatcgcgg acggcaccgt tcgtctgcct gcagccgggg tcgagccggc atcctcgggc
     2401 tcggccgggg tcgagacgat cgaccgcgaa aggccgctga ccccggagaa catgacgggc
     2461 ttcgtatgat cgggcccgtg ccatgatcag gatcgatgat gtccgggcgc ggatcgaggg
     2521 aaaggttccg gcgctggccg gtcagatcgg caacgcgggc gagttctcca agctgatcga
     2581 ggcgggccag atgcaggcgg cccgcaaggc ctatgtcctg cccggggcgc tggccggcgg
     2641 cgcgcggcag gcgatttcgg ggatgttcac ccaggcgctc gaggagacgg tcctggtcgt
     2701 gctgtcggtg cgggtggcga gcgatccgac cggggcgcgg gcgctcgatg agatttcgcc
     2761 gctggtccgc gacaccgtgc gtgcggtctg cggatggggt ccggaggatg ctccgggcgt
     2821 gtttgccctg gcgcgcggcg agctggtcgg cgcggcccag gggatgctga tctaccagct
     2881 cgatttcact ctcgatgacc agttgaggat cacgccatga gcaggaagac cgccgacgcc
     2941 gaagcgccgg ccgccgatat cgaagcgccg gccgccgagc cggtcaccgc gcccgagcag
     3001 ctgccgcaga caggcggctg cttcatccgc aacccggacg ggacgctgac ccgcgacccc
     3061 gcgttccacg gcgaacagga aggctgagcc atgcccaagt tctggagatc gaagaccctg
     3121 ctggccaagg ccgaagcgac ttatggggtc gacccggccc cgaccggcgc ggccaacgcg
     3181 atcctggcga tgaacgtgac gttcaccccg caggaaggcg aagtggtcaa gcgtgcggtg
     3241 gagcggccct atttcggggc gcagccgttc gtcgcccagt cgtttcgtgc ctcgctgtcg
     3301 ttcgaggtcg acctggtcgg ttcgggcgct gccggcaccg cgccggcctg ggggccgctg
     3361 ctgcgggcct gtgccgtggc ccagacggtc actgccgggg tcaaggtcga gtacacgccg
     3421 atcaccgaca gccaggaatc ggtggcgatg tatttcgacg tcgacggcat caagcacgtg
     3481 atgctggggg caaagggcaa cgcggtgatc cgggccgggg tcaacggcaa cccggtgctg
     3541 gcgttcaacc tgatgagcct gttcaccgcg ccggtcgatg cggccaagcc ttcgcccgac
     3601 tataccgcct ggcccgaccc gcaggcggcg agcaaggcca acaccccgac atataccatc
     3661 ggcggcgcgg cgttcgtcgc gcggtcgtat gagcttgatc tcggcaacca ggtcgagccg
     3721 cggatgctga tcggatacga gggcatcctg atcaccggcc gcgaggaaag cctgaagatg
     3781 acggtcgagg ccgtcgcggc ggcaaccttc aacccgcccg ccgtcgtcgc ggccgggacc
     3841 aagctggcca cgtcgctgct gcatggcaca gtcgtcggcc gcaaggtcca ggtcgatacc
     3901 ccggcggcgc agctgctgcc gatcggccag ctcgccgagc agcagggggt cgtcgaatgg
     3961 accctgccgt tcgtgccgct gccggtcacc ggcaacgacc agtggaagat caccctgacc
     4021 tgatcctgac cggacggggg ccgctcgaga agcgtctcag gcggccctca ccaccctttc
     4081 aacgcccgct ttcgaggtcc cgatgttcaa gatcaccacc aatcccacgt tcacccacag
     4141 cgttcatgtc atggttcccg tcgacggcgg gcacaaggaa cagaccttca aggccacctt
     4201 cgcggtgatg gacgtggaag agctggagcg ggtccaggac gaagagggtc agcggggcgt
     4261 gctgcgcaag gtcatcaagg ggttcgatga gctgatcggc gacgacgaca agcccgtgcc
     4321 cttttctgat
//
LOCUS       DATNBV010000018         7615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1610427,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000018 DATNBV010000000
VERSION     DATNBV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610427"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VN627_00445"
     CDS             complement(<1..271)
                     /locus_tag="VN627_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXD01751.1"
                     /translation="MTRIGVLQMTSGIDPARNGATIVAAVAEAARGGAAMLFTPEMSG
                     LVDRDRKRAAAHIVSEDADPVLAAVREAAAAAGIWVALGSLAVKGA"
     gene            complement(273..536)
                     /gene="grxC"
                     /locus_tag="VN627_00450"
     CDS             complement(273..536)
                     /gene="grxC"
                     /locus_tag="VN627_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HXD01752.1"
                     /translation="MAKVEIYTQWGCPYCTRAKALLDGKGVSYTEYDVTMDMTKRKEM
                     AERNPGARTVPQVFIDDVAYGGSDDIHALDRAGKLDAVLGIAG"
     gene            727..1197
                     /locus_tag="VN627_00455"
     CDS             727..1197
                     /locus_tag="VN627_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HXD01753.1"
                     /translation="MNRFDFTPYRRSTVGFDRLFDLLENSSRAASGDNYPPFNIERRG
                     EDAYRITLALAGFKPADLEITAQQNLLVIQGRKGDEQSSGQYLHVGIAQRGFERRFEL
                     ADFVRVENATLEDGLLVIDLVREVPEAMKPKKIAVNGQASLSVVDAPAPTSAAA"
     gene            complement(1662..3308)
                     /locus_tag="VN627_00460"
     CDS             complement(1662..3308)
                     /locus_tag="VN627_00460"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXD01754.1"
                     /translation="MARYIFITGGVVSSLGKGLMAASLAALLQARGYRVRIRKFDPYL
                     NVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGVSARQSDNITSGRIYQQIIAKE
                     RRGDYLGATVQVIPHVTDAIKEFAQADTSSEDGGDLDFVLCEIGGTVGDIESLPFIEA
                     LRQLRNELGREQTCSVHVTLVPYIAAAGELKTKPTQHSVRELTALGVQPDLLLCRCEK
                     PIPDGERAKIALFCNVRKEAVIPALDASSIYAVPLQYHAEGLDNEVLRHFGLTAPAPD
                     LARWEDIVDRYQNPEGEVTIGVVGKYVGLQDAYKSLNEALVHGGMAHRVKVNIRWLDA
                     ELFEQDDTTIAAHLEPMHGILVPGGFGERGTEGKIASVRFARERKVPFFGICLGMQMA
                     CIEGARNTAGIAGASSTEFGPTDEPVVGIITEWMTAEGLETRAEGGDLGGTMRLGAYP
                     ARLAGNSHVSSIYGTTEISERHRHRYEVNCGYRDQLEHGGLVFSGMSPDGLLPEIVER
                     PDHPWFVGVQFHPELKSRPFDPHPLFAGFIGAALLQARLV"
     gene            complement(3496..3852)
                     /gene="secG"
                     /locus_tag="VN627_00465"
     CDS             complement(3496..3852)
                     /gene="secG"
                     /locus_tag="VN627_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXD01755.1"
                     /translation="MFLFLIVVQAIVAALLVGVILMQKSEGGGLGTGGSPAGFMSARG
                     AADFLTRTTAILATAFVVLSIVLAALAANTSGGRTIDTSLQREAPVQAPIDPLAPAQN
                     GANGAAPAQAPAPQAT"
     gene            complement(3993..4763)
                     /gene="tpiA"
                     /locus_tag="VN627_00470"
     CDS             complement(3993..4763)
                     /gene="tpiA"
                     /locus_tag="VN627_00470"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXD01756.1"
                     /translation="MPNRPYIVGNWKMNGTRAMLPEARAIDRAAGRYPAVQVALAPPF
                     TLIQALTSEVTAIGTGGQDCHVQLKGAHTGDISPAMLDDAGADFVIVGHSERRKDHGE
                     SNELVKAKAEAALGCGLGVIVCVGETLAERDAGQAEAVVKAQVSASMPSAALAGETAP
                     GKISVAYEPVWAIGTGRVAAVDDVIAMHRAIRATLTELYGEVGAGVRILYGGSVNADN
                     ARDLLAAEDVGGALVGGASLTAESFLAIVAAAGVPAES"
     gene            5131..7050
                     /locus_tag="VN627_00475"
     CDS             5131..7050
                     /locus_tag="VN627_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXD01757.1"
                     /translation="MRNSKLGVISAIVFLVIVGLSFAAGDISSSNQFGGVAGGDRVAS
                     VGDRKLSTADLTSAANNALEQLKQQNPRLSMRAFVAGGGLEEALGQMIDRTAVSVFGA
                     EHGVVASDRLVDSEITKIPAFQGPDGKFSQAAYKSLLAQRGLNDKAVRQDLADGLVAR
                     QLLVPAAYGARMPVDVVKRYAALLADRRSGTIAMLPAAVFAPKQEPSAAQLAAFYAAH
                     QGAYIRPERRTLRYAVFDETAIKSVTAPTDAEIAARYNVNKALYGPSEERKVSQLVLP
                     SEADARALVASGKGLEAAAAAKGLAVASLGPVSRTQLASQASPTVADAAFTAEKGKLV
                     GPVRSPLGWAVLRVDAIAAKPGKTLDQARAEIAADLSAQKKRTALTDYSAKIEDEFDN
                     GGSLAGVAKELGLAVKETPALTADGKVYGTPGQAAPAELARVVATAFSMEHEGQPQVA
                     EVVPGKTFVVFDVSRIVPSAPAQIAEIRGDVLADYMLEQGSAAAKAAAEKVLAALRKG
                     ATMAAALTGLGQPGVPAPQQLDVNRQQIAQQGQGTPPPLALLFSMAQGTAKVLAAPRS
                     QGWYVVSLAKIVPGSDAEIAPVIPDATRELSTVTGREYAESLRVAMREEVGVKRNQAG
                     IDAVRRQLGGEGSGN"
     gene            7062..>7615
                     /locus_tag="VN627_00480"
     CDS             7062..>7615
                     /locus_tag="VN627_00480"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994041.1"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HXD01758.1"
                     /translation="MTVQPENSPAALAQLTAGKPALVWRRLVADTETPVGAALKLFED
                     GRGDFLLESVQGGEVRGRYSLLGLAPDLVFRATGHACEINRSWTTDRAAFDTLPGDAL
                     VELRALVNACRIDVPAELPPALACLVGYFGYETIGLVEKLPRAPASALELPDMLFVRP
                     TLILVFDRLSDALWCVAPVWATGQ"
BASE COUNT         1236 a         2757 c         2410 g         1212 t
ORIGIN      
        1 ccgcgccctt caccgccagc gaccccagcg ccacccagat ccccgccgcc gccgccgcct
       61 cgcgcacggc ggccagcacc ggatccgcgt cctcgctcac gatatgggcg gcagcgcgct
      121 tgcggtcgcg gtcgaccagg cccgacatct cgggcgtaaa cagcatcgcc gccccgcccc
      181 gcgccgcctc ggctacggcg gcgacaatgg tcgccccgtt gcgcgcggga tcgatcccgc
      241 tggtcatctg gagaacgccg atgcgggtca tctcagcctg cgatccccag cacggcatcg
      301 agcttgcccg cccggtcgag cgcatggata tcgtcgcttc cgccataggc cacgtcgtcg
      361 atgaacacct gcggcacggt ccgcgccccc gggttgcgct cggccatttc cttgcgcttg
      421 gtcatgtcca tcgtcacgtc gtattcggtg tagctcacgc ccttgccgtc cagcagcgcc
      481 tttgcccgcg tgcagtaggg gcagccccac tgggtgtaga tctccacctt ggccatggtt
      541 ctccttcgct cccgcggggg ggcttgcaat cgaccgcaac gcggccaccc cgcaggtctt
      601 gaaacgcggt gcggctaaca tatctcccac tgcgtgtcat gcgccgctcc gggcatgatg
      661 cgctgccggg atgccgcatc agggtcccgt gcaggttcct gaaattgctc agacgaggat
      721 tttaccatga accgtttcga cttcaccccc tatcgccgca gcaccgtcgg cttcgatcgc
      781 ctgttcgacc tgctcgagaa ttcctcgcgg gccgcctcgg gcgacaacta tcccccgttc
      841 aatatcgagc gccgcggcga ggatgcctat cgcatcaccc tggccctggc cgggttcaag
      901 ccggccgacc tcgaaatcac cgcgcagcag aacctgctgg tgatccaggg ccgcaagggc
      961 gacgagcagt cctcgggcca gtatctccac gtcggcattg cccagcgcgg gttcgaacgg
     1021 cgcttcgagc ttgccgactt cgtccgcgtg gagaacgcca cccttgaaga cggtctgctg
     1081 gtgatcgacc tcgtgcgcga agtgcccgag gcgatgaagc ccaagaagat cgcggtgaac
     1141 ggccaggcca gcctcagcgt ggtcgatgcc ccggcgccga ccagcgcagc ggcctgatcc
     1201 gcatatatcc aggaatggct gataaattca ggggcgggtc tgggaggacc cgcccccgcg
     1261 acgcatgagc gccgccttgt tcgttcacga tcgtccgggt cgcaaaagac gatcgatccg
     1321 gaacaagccc gagatgatcg aaccggccga atagaccgga aacctttgta cacatccggc
     1381 aagatcgcca gaccccggga tcaaaacagc aacccctccc gggcaagatg caagcgattg
     1441 catcggcttc tcggggcacg gttgaccggt tgccccccct tttggcaagc catattgcaa
     1501 cgggttcgga gaaatttacc attcactcat gcctgaattt gacatgatat gaaaatcgtt
     1561 acggaattta tttatgtacc ttcgggcaat ttgccgaaag gtaacactct caggactgcc
     1621 tcatcgcggc ctggctgacg ggatcagccg gcccctccgg ttcagaccag ccgcgcctgc
     1681 agcagtgccg ccccgatgaa cccggcaaac aacggatgcg gatcgaaggg ccggctcttg
     1741 agctccggat ggaactgcac gccgacgaac cacggatggt ccggccgctc cacgatctcg
     1801 ggcagcaggc catcgggaga catgcccgaa aacaccagcc cgccatgctc cagctggtca
     1861 cggtagccgc agttgacctc gtagcggtgg cggtgccgct cgctgatctc ggtcgtgccg
     1921 tagatgctgg aaacgtggct gttgcccgcc aacctggccg gataggcccc cagcctcatc
     1981 gtgccgccca ggtccccgcc ctcggcgcgg gtctccaggc cttccgcggt catccactcg
     2041 gtgatgatgc caaccactgg ctcgtcggtc ggcccgaact cggtcgaact ggccccggcg
     2101 atcccggccg tgttgcgggc cccctcgatg caggccatct gcatgccgag gcaaatgccg
     2161 aagaacggca ccttgcgctc acgggcaaag cgcacgctgg cgatcttgcc ctcggtcccg
     2221 cgctcgccaa atccgcctgg cacgagaatg ccgtgcatcg gctcgagatg ggcggcgatg
     2281 gtcgtatcgt cctgctcgaa caactcggca tcgagccagc ggatgttgac cttgacccgg
     2341 tgcgccatgc cgccatggac cagcgcctcg ttaaggctct tgtaggcgtc ctgcaggccg
     2401 acatacttgc cgaccacgcc gatcgtgacc tcgccttcgg ggttctggta gcggtcgacg
     2461 atatcttccc accgcgccag gtcaggggcc ggcgcggtca ggccgaagtg ccgcagcacc
     2521 tcgttgtcga ggccctcggc gtgatactgc agcggcacgg catagatgct gctcgcgtcc
     2581 agcgccggga tcaccgcttc cttgcgcacg ttgcagaaca gcgcgatctt ggcgcgctcg
     2641 ccgtcgggga tcggcttttc gcagcggcac agcagcaggt cgggctgcac gccaagcgcg
     2701 gtcagctcgc gcaccgaatg ctgggtcggc ttggtcttca gctcgccggc cgccgcgata
     2761 tagggcacca gcgtcacgtg caccgagcag gtctgttcgc ggcccagctc gttgcgcagc
     2821 tggcgcaggg cctcgatgaa tggcaagctc tcgatatcgc ccaccgtgcc gccgatttcg
     2881 cacagcacga aatcgagatc gccaccgtcc tcgctcgatg tgtcggcctg ggcgaattcc
     2941 ttgatcgcgt cggtcacgtg ggggatcacc tgaaccgtcg cgcccagata gtcgccgcgg
     3001 cgctcctttg cgatgatctg ctggtagatg cggcctgagg tgatgttatc cgactggcgc
     3061 gccgaaaccc cggtgaaccg ctcgtagtgg ccaagatcca ggtccgtctc cgcgccgtcg
     3121 tcggtgacgt agacctcgcc gtgctggtac gggctcatcg tcccgggatc gacgttcagg
     3181 tagggatcga acttgcggat gcgaacgcga taaccgcgcg cctgcagcag agccgcgagg
     3241 ctcgccgcca tgagaccttt gccgagcgag gagaccacgc cgccggtgat gaaaatgtac
     3301 cgcgccatgg gagtcgggcc ttaatccgtt ctgggggacc cgggcaagcg cggcgagacg
     3361 gtttgcgagg ttaaatccac aagtcgtccc gcccgccgcg cgatccatgc cgcgccgcgc
     3421 cggtattccg gtttaaaaac aagaaagtac cggccgacac agggccgacc ggcaaaaatc
     3481 agtcgtgcag cgggatcagg tcgcctgcgg ggccggagcc tgtgccggag ccgcgccatt
     3541 ggcgccgttc tgggcggggg ccagcggatc gatcggcgcc tgcaccggtg cctcgcgctg
     3601 aagcgaggtg tcgatcgtgc ggccgcccga ggtattggcc gccagggctg ccagtacgat
     3661 cgagagcacc acgaacgcgg tcgcgaggat cgccgtggtt cgcgtgagga agtcagccgc
     3721 gccgcgcgcc gacatgaacc cggccgggct gccgccggtg ccgaggccac cgccttcgga
     3781 cttctgcatc aggatcacgc cgaccagcag cgccgcgacg atcgcctgga caacgatgag
     3841 gaaaaggaac agcgacatgg aaccacgcac atcaatggaa ttgaggggca tctaggcccg
     3901 caaggcgcgc cgcgcaagcc ggggttttcg gcctggtacg gatcgggcat ccgcccgacc
     3961 cgtgcggcac cggcccgctc cggcaagacc gctcagctct cggcggggac tccggccgcc
     4021 gcgacgatgg cgaggaagct ctccgccgtc aggctggcgc cgccgaccag tgccccgccg
     4081 acatcttcgg cggccagcag gtcgcgggca ttgtcggcat tgaccgagcc tccgtagaga
     4141 atgcgcacgc ctgcgccgac ctcgccataa agctcggtca gcgtcgcgcg gatcgcgcgg
     4201 tgcatggcga tgacgtcgtc gaccgccgcc acgcgcccgg tgccgatggc ccagaccggc
     4261 tcgtaggcca ccgagatctt gcccggcgca gtttcgccgg ccagtgccgc cgagggcatc
     4321 gaggcggaga cctgagcctt gaccacggcc tcggcctgcc cggcatcgcg ctcagccagg
     4381 gtctcgccga cgcagacgat cacgcccagg ccgcagccca aggctgcctc ggccttggcc
     4441 ttgaccagtt cattgctctc gccgtgatcc ttgcgccgct cggaatggcc gacgatcacg
     4501 aagtccgccc cggcatcgtc gagcatcgcc ggcgagatgt cgccggtgtg ggcacccttg
     4561 agttgcacgt ggcaatcctg cccaccggtg ccgattgccg taacctcgct ggtcagcgcc
     4621 tggatcaggg tgaacggcgg agccagggcc acctggaccg cgggatagcg gccggcggcg
     4681 cggtcgatcg cgcgggcctc gggcagcatc gccctcgtcc cgttcatttt ccagtttcca
     4741 acgatgtacg gcctgttcgg catgacggat ccctgcatag ctttgttgtg ccggcccggt
     4801 cacttgacgg ccggcacggc gagcccgggc gattctgccg gacgggtctt gggcgcgctg
     4861 ctaggccacc catggccgcc ctgtcaaaaa cagcctgtgc attttcagcc ccgcagccgc
     4921 gaaaaacttg taatttttcg ccagacgtcg ggcaaccttg ctgtcgggca ccatgcgccc
     4981 cccacccgcc attcctccac gaagcccgca cccacgcggt tgcgaagggc gcgattggct
     5041 cctaaagccg cgcgaaccgc gggcaattgt cggcccgcga cgaatccagc cgaggcgata
     5101 gaccgctcca tgctcaaact tttccgctcc atgcgtaatt ccaagctggg cgtcatctcg
     5161 gcgatcgtgt tcctggtaat cgtcgggctg tccttcgcgg cgggcgatat ttcgagcagc
     5221 aaccagttcg gcggcgtcgc cgggggcgac cgggtggcca gcgtgggcga ccgcaagctc
     5281 tccaccgccg atctcaccag cgcggccaac aacgcgctcg aacagctcaa gcagcagaac
     5341 ccgcgcctgt cgatgcgcgc cttcgtcgcc ggcggcggcc ttgaggaagc cctcggccag
     5401 atgatcgacc gtacggccgt atctgtcttc ggggcagagc acggcgtggt cgccagtgac
     5461 cggctggtgg acagcgagat caccaagatc cccgccttcc agggccccga cggcaagttc
     5521 agccaggcgg cctacaagtc gctgctcgcc cagcgcgggc tcaacgacaa ggccgtgcgc
     5581 caggaccttg ccgatggcct cgtcgcgcgc cagctcctcg tccccgccgc ctatggcgcg
     5641 cgcatgccgg tggacgtggt caagcgctac gccgccctgc tggccgatcg gcgcagcggc
     5701 accatcgcca tgctgccggc cgccgtcttc gcgcccaagc aggagccgag cgcggcacag
     5761 ctcgccgcct tctacgcggc ccaccagggc gcctatatcc ggcccgagcg ccgcaccctg
     5821 cgctacgccg tgttcgacga gaccgcgatc aagtccgtta ccgcccccac cgatgccgaa
     5881 atcgccgccc gctacaacgt gaacaaggcg ctctacggcc cgagcgagga gcgcaaggtt
     5941 tcgcagctgg tcctcccctc ggaagccgat gcgcgggcgc tcgtcgcctc gggcaaggga
     6001 ctcgaagccg ccgccgccgc caagggcctc gccgtcgcct cgctgggccc tgtcagccgc
     6061 acgcagctgg cgtcgcaggc aagcccgacc gttgccgatg ccgccttcac ggccgaaaag
     6121 ggcaagctcg tcggcccggt ccgcagcccg ctcggctggg ccgtgctgcg ggtcgatgcc
     6181 atcgctgcca agccgggcaa gacgctcgac caggcccgcg ccgaaatcgc cgccgacctt
     6241 tcggcccaga agaagcgcac cgccctgacc gactattcgg ccaagatcga ggacgagttc
     6301 gacaatggcg gctccctcgc cggcgtggcc aaggaactcg gtctggccgt caaggaaacc
     6361 ccggcgctga ccgccgacgg caaggtctac ggcacgccgg ggcaggccgc gccggccgaa
     6421 ctcgcccgcg tcgtcgccac cgccttctcc atggagcacg aaggccagcc gcaggtcgcc
     6481 gaagtggtgc cgggcaagac cttcgtggtc ttcgacgtat ccaggatcgt cccctcggcc
     6541 ccagcgcaga tcgccgaaat ccgcggcgat gtcctggccg actacatgct cgagcagggc
     6601 tccgccgccg ccaaggccgc cgctgaaaag gttctcgccg cgctgcgcaa gggcgccacc
     6661 atggccgccg ccctcaccgg cctcggccag cccggcgtcc ccgcgccgca gcagctcgac
     6721 gtcaaccgcc agcagatcgc ccagcagggc cagggcaccc cgccgccgct cgccctgctg
     6781 ttctccatgg cgcagggtac ggccaaggtt ctcgccgcgc cgcgcagcca gggctggtac
     6841 gtcgtcagcc tggcgaagat cgttcccggc agtgatgccg agatcgcccc ggtcatcccc
     6901 gatgccaccc gcgagctctc gaccgtcacg ggccgcgaat acgccgaatc cctgcgcgtc
     6961 gcgatgcgcg aggaagtcgg cgtcaagcgc aaccaggccg gcatcgatgc cgtgcgccgc
     7021 caactcggcg gcgagggcag cggcaactga tcacccgcgc gatgaccgtc cagcccgaaa
     7081 acagtcctgc cgccctcgcc cagctcaccg ccggcaagcc cgcgctcgtc tggcgccggc
     7141 tcgtcgccga tacggaaacc ccggtcggcg ccgccctcaa gctgttcgag gacgggcgcg
     7201 gcgatttcct gctggagtcg gtccagggcg gcgaagtccg cggccgctat tcgctgctcg
     7261 gcctcgcgcc cgacctggtc ttccgcgcca ccggccatgc ctgcgagatc aaccgcagct
     7321 ggacgaccga ccgcgccgcc ttcgacaccc tccccggcga tgccctcgtc gagctgcgcg
     7381 cgctggtgaa cgcctgccgg atcgacgtgc ccgccgagct ccccccggcc ctcgcctgcc
     7441 tcgtcggcta tttcggctat gagacgatcg gcctcgtcga aaagctcccc cgcgccccgg
     7501 cctcggcgct cgaactgcct gacatgctgt tcgtccggcc gaccctgatc ctggtgttcg
     7561 accgcctctc ggatgccttg tggtgcgtcg ccccggtctg ggccaccggc cagga
//
LOCUS       DATNBV010000019         2984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4165657,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000019 DATNBV010000000
VERSION     DATNBV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4165657"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VN627_00485"
     CDS             complement(<1..239)
                     /locus_tag="VN627_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01759.1"
                     /translation="MKALLIASTALIMIATPASAQLLGGGLGGSLGGGLTGGLGGTMG
                     SVGGMTNGTLSGAASSSGTQSVDRHHGSVAVDRSV"
     gene            complement(342..1565)
                     /locus_tag="VN627_00490"
     CDS             complement(342..1565)
                     /locus_tag="VN627_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXD01760.1"
                     /translation="MRVIFILLLGLAGPAASQIGLPSVGQTLGSTVDAVGRTVDRASG
                     LDLDGAVGSVTRLAHDRLARLTGFVRTHGDRVEFDDVGAPARRGVVLLVDADNMSLEA
                     AQKLGFSSSGVQQLGNLGLATAELAVPAGVPLRRALVLLRKALPGKTLVADQLHFPSG
                     SQDIVAAKRAGAMMPPIRTLVGLIDGGVANTVPVARTRGFATGGPAPSNHGTATASLL
                     RYAGVTNIAAADVYGRDPAGGSALAIARALDWMAGEKVPVISISLVGPRNPLLERAIA
                     ALSARGTVIVAAVGNDGPAAPPAFPASYAPVMAITGVDGRNRALIEAGRALHLDYAAP
                     GADIMAADAQGRSRPVRGTSFAAPLAAARAAAMIDRGKGGAALRGALDAEARDLGKKG
                     PDASFGRGLLCGDCR"
     gene            1620..2150
                     /locus_tag="VN627_00495"
     CDS             1620..2150
                     /locus_tag="VN627_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXD01761.1"
                     /translation="MAPPVSSHRREARVLSSSFEDGILALLPRLRRFAASLSRNLDDG
                     DDLCQMTIERALKARDQWAEGTRLDSWVYRIMRNIWIDEIRARSRRQQTFVGEEQGLE
                     AGAPPEQESRVELSMVDRAMAQLAVEQREAILLVMVEGYGYREAAEIIGCPIGTLTSR
                     LLRGRDALQQLLGETA"
     gene            2147..2878
                     /locus_tag="VN627_00500"
     CDS             2147..2878
                     /locus_tag="VN627_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXD01762.1"
                     /translation="MTIDRELLAAYADGELGPDEVARVEAAIAADPALAAELDAHRQL
                     RATLSSHFAPILDMPVPDRLSAALKTSSNVVDLGEVRRAREATRSLSNIPSRWVMGGA
                     IAASLVVGVMLGGQLPTGAPSSSNGGQLIASGPLDKALTTQLASAGGRAPVRVLLSFK
                     AGDGRFCRAFEAPALSGIACRDDKNWTILRTQAGGTQSHADYRQAGSAAAGLMAEAQA
                     MAPGGALDEQAERAARDGGWAPKTR"
BASE COUNT          504 a         1016 c         1015 g          449 t
ORIGIN      
        1 tcgaccgagc ggtcaaccgc gaccgagccg tgatgacgat cgacgctctg ggtgccgctg
       61 ctgcttgccg cgccggagag cgtgccattg gtcatgccgc cgacgctgcc catggtgccg
      121 ccgaggccgc cggtcaggcc gccgccaagc gatccgccaa ggccgccgcc caaaagttgg
      181 gccgaggcag gtgtggcgat cattataagt gccgtagagg cgatcaaaag tgccttcatg
      241 atgaattcct ttccgtctgc cgagcgcgat gcggcgctct catcaaggct aacggaccgg
      301 gttcgcggat taattcatgg ggcgcagaaa ttttttcagc gctaccggca atcgccgcac
      361 aacaggccgc gtccaaagct ggcgtcgggg cccttcttgc cgagatcgcg cgcttcggcg
      421 tccaaggccc cccgcagcgc cgcgccgccc ttgccccggt cgatcatcgc ggcggcccgg
      481 gcggcggcta ggggtgccgc gaaggacgtg ccacgcactg ggcggctgcg cccctgcgca
      541 tcggccgcca tgatgtcggc gccgggcgcg gcatagtcga ggtgaagcgc ccgtccggcc
      601 tcgatcagcg cgcgattgcg gccatcgaca ccggtgatgg ccatgacggg cgcgtaggat
      661 gcgggaaagg ctggcggggc ggcgggtcca tcattgccca ccgccgcgac gatcacggtg
      721 ccgcgcgccg acagcgccgc gatggcgcgc tccaggagcg gattgcgcgg gccgacgagg
      781 ctgatcgaga tcaccgggac cttctccccc gccatccagt cgagggcgcg cgcaatggcc
      841 agcgcgcttc cgccggccgg gtcacggcca tagacatcgg cggccgcgat attggtcacc
      901 ccggcatagc gcagcagcga cgcggtcgcg gtgccatgat tgctgggcgc gggaccgccg
      961 gtcgcaaagc ctcgggtgcg ggcaaccggg acggtattgg ccacaccgcc gtcgatcagc
     1021 ccgaccaagg tccggatcgg cggcatcatt gcccccgccc gtttcgccgc cacgatatcc
     1081 tggctcccgc tcgggaaatg cagctgatcg gcgaccagtg tcttgccggg cagcgccttg
     1141 cgcagcagga caagcgcgcg gcgcagcggc acgcccgcag gcacggccag ttccgccgtg
     1201 gccagcccca gattgccgag ctgctggacg ccactgctcg aaaagcccag tttttgcgca
     1261 gcctccagcg acatattatc tgcatccacc agcagcacga cgccccgacg tgcgggcgcg
     1321 cccacatcat cgaactcgac cctgtcgcca tgtgtccgga caaagccggt gaggcgcgcg
     1381 aggcgatcgt gcgccagtcg ggtcaccgat cccaccgccc cgtccagatc gagacccgac
     1441 gcccggtcga cggtcctgcc cactgcatcg acggtcgaac ccagcgtctg gccaaccgac
     1501 ggcagcccga tctggctggc ggccggaccg gcgagcccga gcagcaggat gaagatgacg
     1561 cgcactcgca taagccttat gcctatcgtg ataaaatata ctttgcatgg atgaaacgca
     1621 tggccccgcc cgtttcatcc cacagacgag aggcacgggt tctgagttcg agcttcgagg
     1681 acggcatttt agccctgctg ccacggctcc gccggtttgc ggcgagcctg tcgcgcaacc
     1741 tcgatgacgg cgacgacctg tgccagatga cgatcgagcg cgcgctcaag gcccgtgacc
     1801 aatgggcgga gggcacgcgg ctcgacagct gggtatatcg gatcatgcgc aacatctgga
     1861 ttgacgaaat acgcgcccgc agccgcaggc agcagacctt tgtgggggag gagcaggggc
     1921 ttgaggccgg cgcgccgccc gagcaggaaa gccgcgtcga actcagcatg gtcgaccgcg
     1981 caatggcgca gctggccgtc gagcagcgcg aggcgatcct gctggtgatg gtcgagggct
     2041 atggctaccg cgaggccgcc gagatcatcg gctgcccgat cggcacattg acctcgcggc
     2101 tccttcgggg ccgcgatgcc ttgcaacagc tgttgggaga gacggcgtga ccatcgatcg
     2161 agaactgctg gccgcctatg ccgatggcga actcggcccc gacgaggttg cgcgggtgga
     2221 agcggcgatc gccgccgatc ccgccctggc cgcggaactg gacgcgcacc gccagttgcg
     2281 cgcgaccctg agcagccact tcgcgcccat tctcgacatg ccggttccgg accggttgag
     2341 cgcggcgctc aagacatcct ccaatgtcgt cgatctgggc gaggtccgcc gagcgaggga
     2401 agcaacgcgg agcctatcca atattccctc gcgctgggtc atgggcgggg cgatcgcggc
     2461 atcgcttgtg gtcggtgtca tgctgggcgg acagcttccc accggcgcgc cgagcagcag
     2521 caatggcggc cagctcatcg cgagcggccc attggacaag gcgctgacca cgcaactggc
     2581 ctcggcggga gggcgtgcgc cggtgcgcgt cctgctctcg ttcaaggccg gcgacgggcg
     2641 tttttgtcgg gccttcgagg ccccggcgct ctccggcatc gcctgccgcg acgacaagaa
     2701 ctggacgatt ttgcgcacgc aggcaggcgg aacgcaaagc catgcggact atcggcaggc
     2761 gggctccgcg gccgccggtc tcatggcgga ggcgcaggcg atggcgcccg gcggcgcgct
     2821 cgacgaacag gccgaacgcg cggcccgaga cgggggctgg gcacctaaaa cccgctagcc
     2881 gtccagggtg aagcccacct tcatagtgac ctggaattgt tccacgcgcc cgtccttgac
     2941 atggccggtg gtcttgagga tctcgaacca tcccaaatgt cgca
//
LOCUS       DATNBV010000020         7709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2886111,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000020 DATNBV010000000
VERSION     DATNBV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2886111"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VN627_00505"
     CDS             <1..983
                     /locus_tag="VN627_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXD01763.1"
                     /translation="KTPGVTRASPLIEQPLLATFNGRVEGILVRGNTQADIDRIKPQV
                     KDGSLQLLRPGSNMVAIGARLAENLGARVGDTITIINPQGRATPFGTVPRQVGYTVGA
                     IFEIGVYDYDNTFVVMPIPDAQTLLLSGDSIGMIEVKTTDPDHVDEILAPLARKLAGV
                     AVVTDWKTINAALFEALSVERVAMFTVLSIIVLVAVFNILSSLIMLVRAKTRDIAILR
                     TMGARRNSLLKIFVTIGFSIGALGTGVGLLLGFVFLFFRQNVVNAVQYLTGQNLWDPS
                     IRFLSELPSRSDPVEIVAIAVMALVLSFLATLYPAWKAASTDPVQVLRYE"
     gene            976..1650
                     /locus_tag="VN627_00510"
     CDS             976..1650
                     /locus_tag="VN627_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXD01764.1"
                     /translation="MNDPVVRLAGVTRSFEQGGVRIDVLRGIDLDVRPGEIVALLGPS
                     GSGKSTMLQAIGLLEGGFGGRIEIAGVDASAEDSAGRTTLRREHLGFVYQFHHLLPDF
                     TAIENVILPQLIAGKSQHEAESRATELLESLGLGQRLTHKPSRLSGGEQQRVAVARAL
                     ANRPALVLADEPTGNLDEATADKVLAQFLSLVRGEGSAALIATHNERLALAMDRVVRL
                     HEGKLT"
     gene            1727..2011
                     /locus_tag="VN627_00515"
     CDS             1727..2011
                     /locus_tag="VN627_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01765.1"
                     /translation="MSHHPTTFAAEASDEAPGTIHWDDSATLHRRADGGGLLHGFKAI
                     RKGTFGELVRYVAAVPESERSHYMIEKAGDRQYLPHEIMKLAQRADLPLT"
     gene            2017..2502
                     /locus_tag="VN627_00520"
     CDS             2017..2502
                     /locus_tag="VN627_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HXD01766.1"
                     /translation="MNAHTIADFTATLPSGREISLADRQGKVLLVVNTASKCGFTPQY
                     AGLEKLWEEFGPRGFEVLAFPCNQFGHQEPGSADEIAQFCQVNFGLSFPLMGKVEVNG
                     ESATPLYRWMKDEAPGLLGTKAIKWNFTKFLIDRQGKVVRRYAPTDKPEALRADIERL
                     I"
     gene            2598..6152
                     /gene="dnaE"
                     /locus_tag="VN627_00525"
     CDS             2598..6152
                     /gene="dnaE"
                     /locus_tag="VN627_00525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXD01767.1"
                     /translation="MAAYNPFVPLRVFSSYSMLEGAIDPKAIAKLAKERGFPAIAVCD
                     RNGLYAAPAFASACKDAGIQPLIGTLLAVAREGSDAIDWLALYAQSEAGWLNLCHLVS
                     RAHLERPLEREPHVQLADLEGHTDGVIALTGAGEGALARLLAGGKAEAAHDLAAHLSA
                     LFPGRLYVEIARRGDATEEASEDALIELAYARDLPLVATNPAMFAESQFYAAHDAMLC
                     IANSTHIDAPDRPRSSGEAWVKPADMMAELFEDLPEATANTLVVARRCAYAPPKRKPL
                     LPSLAGDKEGEARMLIDDARLGLARRLEPYYPNAAQAELVEALRAEGEARVAAFDRLR
                     PGGVGEEFLEYAERLDFETGIINKMGFAGYFLIVADFIKWAKDNDIPVGPGRGSGAGS
                     LVAWSLTITDLDPIRLGLLFERFLNPERVSMPDFDIDFCETRRGEVIRYVQGKYGADH
                     VAQIITFGKLKARAVLRDTGRILQMSYGQVDRLCKLVPNLPADPWDLKRTLNGSAEFR
                     REYDNDDEVKRLVDLAVQLEGLPRNSSTHAAGVVIGDRPLAQLVPLYRDPRSDMPVTQ
                     FDMKYVEDTGLIKFDFLGLKTLSVLRKAVDLLARRGIEIDLGALAWDDPQVYELLQRG
                     DTVGVFQLESEGMRRTLAAVKPTNFGDIIALVSLYRPGPMDNIPLFGRRKNGLEAIEY
                     PHEKLAGILSETYGIFVYQEQVMQAAQILAGYSLGDADLLRRAMGKKVQAEMDAQRQR
                     FVEGCARDSGIDAKKANELFDLIDKFAGYGFNKSHAAAYALLAYQTAWFKAHYPHEFY
                     AAAMCFDMHQSEKLSVFVDDMRRNGLVIAGPDLNRSEAEFTVEQTEDGHQVRYALAGI
                     RNVGEKAMEQIVAEREKNGLFQSLDDLFSRAPQGALNRRALEGLAAAGAFDSLEPNRA
                     KVMANADMLLAVADEAERTRTSGQAGLFGGEGHHQAVRLVETPAWSRAEQMAKERENF
                     GFYFAAHPVEQWRAIASANGARTYGSLMASGGSGGGGRQMAVMAAMVEGVQRRKTKRG
                     KDFVMADFSDSSGQFSASCFEESLVESFVQWAREGTCILLNVELDSPSPEEPPRVTVR
                     GGRPLSEVREAARMELRLDITAPEALGELALLLQPGSPGKGEVVARLRTGGPREPLVR
                     LGRDFHLDGDLAEQVAAIPGVAAVGLSAKRGPSLRLVA"
     gene            6154..6561
                     /locus_tag="VN627_00530"
     CDS             6154..6561
                     /locus_tag="VN627_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HXD01768.1"
                     /translation="MDYVDPSRENFARFKALARDTPIHLLNQIRYRDKAVYPEGHPAA
                     GLGWSGEQAFAEYFRLVVPRIEALGGGLVWNASFECMMTGPAEPEWDRIFVMAFPEAS
                     GFLALVTDPDYKANVVAHRTAAVRDSRLIRYRP"
     gene            complement(6569..7420)
                     /locus_tag="VN627_00535"
     CDS             complement(6569..7420)
                     /locus_tag="VN627_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD01769.1"
                     /translation="MALDQQGKVAIVTGAGGGIGRAEALRLAADGAAVVVNDFNPKAA
                     EETAALIRANGGQAAVAAGDVASLDLAEAAVRTAVEQFGRLDVLINNAGNSRPRFIVN
                     MSEEDWDSVMDTHVKGTFAFTRAAARHWQAEWKRTGQPVSAAIVNTTSINGLHGEPGY
                     VNYVAAKGGIASITVMLAKELGGFGVRVNAIAPLARSGMSAPLVGSDVFPDDRADELS
                     PEMVAEVVGWLASPRSAPLTGEIVNFSGARLNTVHEWPEKGSATSPDRLWDYAKLDAV
                     RASLFAE"
     gene            complement(7521..>7709)
                     /locus_tag="VN627_00540"
     CDS             complement(7521..>7709)
                     /locus_tag="VN627_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01770.1"
                     /translation="PGVMREALGTTSAAPVLSTVNAEVTRQATMVAYTATFRDMALLN
                     FAMLPLLLLLRRRGPGRN"
BASE COUNT         1288 a         2563 c         2558 g         1300 t
ORIGIN      
        1 agaagacgcc gggcgttacc cgcgcctcgc cgctgatcga gcagccgctg ctggcgactt
       61 tcaacggccg ggtcgagggc atactcgtgc gcggcaatac ccaggccgat atcgaccgga
      121 tcaagccgca ggtgaaggac ggttcgctgc aactgcttcg gccgggctcc aacatggtcg
      181 ccatcggcgc gcggctggcg gaaaacctcg gcgcgcgcgt gggcgacacg atcacgatca
      241 tcaatccgca gggccgtgcc acgccgttcg gcacggtgcc ccgccaggtt ggctacaccg
      301 tcggcgcgat attcgagatc ggggtctacg actatgacaa caccttcgtc gtcatgccca
      361 tccccgatgc gcagaccctg ctgctgagcg gcgattccat cggcatgatc gaggtgaaga
      421 cgaccgaccc cgaccatgtc gacgaaatcc tcgccccgct cgcgcgcaag ctggccggcg
      481 ttgcggtggt caccgactgg aagacgatca acgccgccct gttcgaggcg ctgtcggtgg
      541 agcgggtggc gatgttcacc gtgctctcga tcatcgtgct ggtggcggta ttcaacatcc
      601 tgtcctcgct gatcatgctg gtgcgggcca agacgcgcga catcgcgatc ctgcggacga
      661 tgggcgcgcg gcgcaattcg ctgttgaaga tcttcgtgac catcggcttt tcgatcggcg
      721 cgctgggcac gggcgtgggc ctgctgctcg gcttcgtgtt cctgttcttc cggcagaacg
      781 tggtgaacgc cgtgcagtat ctcaccgggc agaacctgtg ggacccgtcg atccggttcc
      841 tgtccgaact gccgagccgc tcggatccgg tggagatcgt ggcgattgcg gtgatggcac
      901 tggtgctctc gttcctcgcc acgctttacc cggcgtggaa ggcggcgagt accgatccgg
      961 tgcaggtgct gcgctatgaa tgatccggtt gtgcggctcg ctggcgtgac ccgcagcttc
     1021 gaacagggcg gggtgcggat tgatgtcctc aggggtatcg atctggacgt gcgccctggc
     1081 gagatcgtcg ccctgcttgg gccttcgggc tcgggcaagt cgaccatgct gcaagcgatc
     1141 gggctgctcg agggcgggtt cggcggcagg atcgagatcg ccggcgtgga tgcctcggca
     1201 gaggattcgg ccggaaggac gacgctgcgg cgcgagcacc ttggtttcgt ctaccagttc
     1261 catcatctgc tgcccgattt cacggccatc gagaacgtga tcctgccgca gctgatcgcc
     1321 ggcaaatcgc agcacgaagc ggaaagccgc gcgaccgagt tgctcgaaag cctcggtctt
     1381 ggccagcggc tcacccacaa gcccagccgg ctgtccggcg gcgagcagca gcgggtggcc
     1441 gttgcccggg cgctcgccaa tcgcccggcg ctggtcctcg cggacgagcc aaccggcaat
     1501 ctcgacgaag cgacggcgga caaggtgctc gcccagttcc tcagccttgt gcgcggggag
     1561 ggtagcgcgg ccctgatcgc tacacacaac gagcgtctgg ccttggccat ggaccgggtg
     1621 gtgcggctgc acgagggcaa gcttacgtaa agttcaccgg aacctgccgg gcggggcaac
     1681 cgttttctct ttgccgggaa caacgggagc caaggaggtc cgttacgtga gccaccatcc
     1741 aaccacattt gccgccgaag cctcggacga agcaccgggc actattcatt gggacgacag
     1801 cgctacgctc catcgccgcg cagacggcgg cggcctgctg catggcttca aggcgatccg
     1861 caagggcact ttcggcgaac tggtgcgcta cgtcgcggcc gtccccgaga gcgaacgcag
     1921 tcattacatg atcgagaagg ccggtgaccg gcagtacctg ccgcacgaaa tcatgaagct
     1981 ggcgcagagg gccgatctgc cgctaacctg agcggcatga acgcacacac aatcgccgat
     2041 ttcacggcaa ccctgccgag cggccgggag atcagtcttg ccgatcggca gggcaaggtc
     2101 ctcctcgtcg tcaacacggc cagcaaatgc ggcttcaccc cgcaatatgc cgggctggag
     2161 aagctgtggg aggagttcgg cccgcgcggg ttcgaggtgc tggcctttcc ctgcaaccag
     2221 ttcggccacc aggagcccgg ttcggccgac gagatcgccc agttctgcca ggtgaacttt
     2281 gggctatcgt ttccgctgat gggcaaggtc gaggtgaacg gcgagagcgc aacgccgctc
     2341 taccgctgga tgaaggacga ggccccgggg ctgctcggca cgaaggcgat caagtggaat
     2401 ttcaccaagt tcctgatcga ccggcagggc aaggtcgtgc gccgctatgc gccgaccgac
     2461 aagcctgagg cgctgcgcgc ggatatcgag cggctgattt gatgatcctc cgcgcatgcg
     2521 gggaggatgt gcgttttcaa caatgaccgg tgggccagcc cttggtccgg cgctgtccac
     2581 tcgctacctt tgggtcgatg gccgcctaca accccttcgt gcccctgcgc gtgttctcca
     2641 gctattccat gctggagggc gcgatcgatc cgaaggcgat tgccaagctg gccaaggagc
     2701 gcggatttcc ggcgatcgcg gtgtgcgatc gcaatgggct ctatgccgcg cccgccttcg
     2761 cgtcggcttg caaggatgcc ggcatccagc cgctgattgg caccctgctg gctgtcgcgc
     2821 gcgagggcag tgacgcgatc gactggctgg cgctctatgc acagagcgaa gccggctggc
     2881 tcaacctctg ccacctcgtc agccgcgccc atctggaacg cccgctggag cgggagcctc
     2941 acgtccagtt ggccgatctc gaaggccaca cggatggcgt gatcgccttg accggtgccg
     3001 gcgagggcgc gctcgcccgc ctgctggccg gtggcaaggc cgaggcggcg catgacctgg
     3061 ccgcgcacct gtcagcactg ttccccgggc ggctctacgt cgagatcgcc cggcgcggcg
     3121 atgccaccga ggaagcctcg gaagatgcgc tgatcgaact ggcctatgcc cgcgatcttc
     3181 cgctcgtcgc cactaacccg gcgatgttcg ccgaaagcca gttctacgcc gcgcacgatg
     3241 ccatgctgtg catcgccaat tccacgcaca tcgacgcgcc cgaccggccg cggagttcgg
     3301 gcgaagcctg ggtcaagccg gccgacatga tggccgaact gttcgaggac ctgcccgagg
     3361 caacggccaa caccctcgtc gtcgcccggc gctgcgccta tgcgccgccc aagcgcaagc
     3421 cgctgctccc cagccttgcc ggcgacaagg aaggcgaagc gcggatgctg atcgacgacg
     3481 cgcgccttgg cctcgcgcgc cgcttggagc cgtattaccc caacgccgct caggctgagc
     3541 ttgtcgaagc tcttcgcgcc gagggcgagg cgcgggtggc ggcttttgac aggctcaggc
     3601 cgggcggcgt tggcgaagaa ttcctggaat atgccgagcg cctcgatttc gagaccggca
     3661 ttatcaacaa gatgggcttt gccggctact tcctgatcgt ggcggacttc atcaagtggg
     3721 ccaaggacaa cgacattccg gtggggccgg ggcgcggctc gggcgcgggc tcactggtcg
     3781 cctggtctct caccatcacc gacctcgatc cgatccgcct cggcctgctg ttcgaacgct
     3841 tcctcaatcc ggaacgcgtt tccatgcccg acttcgatat cgacttctgc gaaacccgcc
     3901 ggggcgaggt gatccgctac gtgcagggca agtatggcgc cgatcacgtt gcccagatca
     3961 tcaccttcgg caagctgaag gcccgcgccg tgctgcgcga taccgggcgc atcctgcaga
     4021 tgagctacgg gcaggtggac cgcttgtgca agctcgtgcc caacctgccg gccgatccgt
     4081 gggatctcaa acgcacgctc aacggctcgg ccgagttcag gcgcgagtat gacaacgacg
     4141 acgaggtgaa gcgcctcgtc gatctcgccg tccagctcga gggcctgccg cgcaacagct
     4201 cgacccatgc cgccggcgtc gtcatcggcg atcgtcccct ggcgcagctc gtcccgcttt
     4261 accgcgatcc gcgctccgac atgccggtga cccagttcga catgaaatat gtcgaggaca
     4321 ccggcctcat caagttcgac ttcctcggcc tgaagaccct ttcggtcctg cgcaaggcgg
     4381 tggacctgct ggcgcggcgc gggatcgaga tcgatctggg cgcgctcgcc tgggacgatc
     4441 cgcaggtcta cgagttgctc cagcgcggcg acacagtggg cgtgttccag ctggaatcgg
     4501 agggcatgcg ccgcaccctt gcggcggtga agccgaccaa cttcggcgac atcatcgcgc
     4561 tggtctcgct ctaccgcccg ggcccgatgg acaacattcc cctgttcggc cgccgcaaga
     4621 acggcctcga ggcgatcgaa tacccgcatg agaagctggc cggcatcctc tcggaaacct
     4681 acgggatctt cgtctaccag gaacaggtca tgcaggccgc gcagatcctc gccggctact
     4741 cgctgggcga tgccgacctg ctgcgccgcg ccatgggcaa gaaggtccag gccgagatgg
     4801 acgcccagcg ccagcgcttc gtcgaaggct gcgcgcggga tagcgggatc gatgcgaaga
     4861 aggcgaacga gctgttcgac ttgatcgaca agttcgccgg ctacggcttc aacaagagcc
     4921 acgccgccgc ctacgccttg ctggcctacc agaccgcctg gttcaaggcg cactatccgc
     4981 acgaattcta cgccgcggcc atgtgcttcg atatgcacca gtcggaaaag ctctcggtct
     5041 tcgtcgacga catgcgccgc aacggcctgg tcatcgccgg gcccgatctc aaccggagcg
     5101 aggccgagtt cacggtggag caaaccgagg acggacatca ggttcgctat gccttggcgg
     5161 gcatccgcaa cgtcggcgag aaggcgatgg agcagatcgt tgccgagcgc gagaagaacg
     5221 gcctgttcca gagccttgac gacctgttca gccgcgcccc ccagggcgcg ctcaaccgac
     5281 gggcgctgga aggcctcgcc gcggccggag ccttcgacag cctcgagccg aaccgggcca
     5341 aggtcatggc caatgctgac atgctgctcg ccgtggccga cgaggccgag cgcacgcgca
     5401 ccagcgggca ggccggtctg ttcgggggcg aggggcacca ccaggccgtc cggctcgtcg
     5461 agaccccggc gtggagccgg gccgagcaga tggccaagga acgcgagaac ttcggcttct
     5521 acttcgccgc gcacccggtc gagcagtggc gcgcgatcgc ctcggcgaac ggggctcgca
     5581 cttatggcag cctgatggcg tcgggtggtt cgggtggagg ggggcgccag atggcggtca
     5641 tggcggcgat ggtcgagggc gtgcagcgcc gcaagaccaa gcgcggcaag gacttcgtca
     5701 tggccgattt ctccgacagt tcgggccagt tctccgcctc gtgcttcgag gaatcgctgg
     5761 tcgaaagctt cgtgcaatgg gccagggaag gcacctgcat cctgcttaac gtcgagctcg
     5821 acagcccgag cccggaagaa ccgccgcgcg tcactgttcg tggcggccgg ccgttgtcgg
     5881 aagtgcgcga ggcggcgcgg atggagttgc ggctggacat caccgcgccg gaggcgctgg
     5941 gcgagctggc cctgctgctc cagcccggat cgccggggaa gggcgaggtt gtcgcccggc
     6001 tgcgtaccgg cggcccgcgt gaaccgctgg tccggctagg gcgcgatttc cacctcgacg
     6061 gcgacttggc cgaacaggtc gccgccatac ccggcgttgc cgccgtgggc ctttcggcca
     6121 agcgcgggcc ctcattgcgg ctggtggcct gagatggact acgtcgatcc ctcgcgcgag
     6181 aacttcgccc ggttcaaggc gctggcgcgc gacacgccga tccacttgct taaccagatc
     6241 cgctaccgcg acaaggccgt ctatcccgag ggccaccccg ccgctgggct gggctggagc
     6301 ggggagcagg ccttcgccga atacttccgc ctcgtcgtcc ctcggatcga ggcgctcggc
     6361 ggcgggctgg tgtggaacgc cagcttcgaa tgcatgatga ccggccccgc cgaacccgag
     6421 tgggatcgga tcttcgtcat ggcctttccg gaggccagcg gcttcctcgc cctggtgacc
     6481 gatcctgatt acaaggccaa tgtcgtggcc caccgcacgg ccgccgtgcg cgacagccgc
     6541 ttgatccgct accgcccctg atcgcccgct attcggcgaa caggctcgcg cgcaccgcgt
     6601 ccagcttggc atagtcccac agccggtcgg gcgaggtggc gctgcccttt tccggccatt
     6661 cgtgaaccgt gttgagccgc gcgcccgaga agttgacgat ctcgccggtg agcggggcgg
     6721 aacggggcga ggccagccag ccgacgacct cggcaaccat ctccggcgaa agttcgtcgg
     6781 cccgatcatc ggggaacacg tcgctgccga ccagcggcgc ggacatgccg ctgcgggcga
     6841 gtggcgcaat ggcgttcacc cgcaccccga agccgcccaa ctccttggcc agcatgacgg
     6901 tgatgctggc gatgccgccc ttcgcggcaa cgtagttcac atagcctggc tcgccatgga
     6961 ggccgttgat cgaggtggtg ttcacgatgg cggcactgac cggctgcccg gtgcgcttcc
     7021 actccgcctg ccagtgccgt gcggcggccc gggtgaaggc gaaggttccc ttgacatggg
     7081 tgtccatcac gctgtcccaa tcttcctcgc tcatgttgac gatgaagcgg ggacgcgaat
     7141 ttccggcatt gttgatcagc acgtcaagcc ggccaaactg ctcgaccgcc gtgcgcacgg
     7201 ctgcctcggc gagatcgagc gaggcgacat ccccggccgc gaccgccgcc tgaccgccgt
     7261 tggcccggat caatgcggca gtttcctcgg cggcctttgg attgaagtcg ttgacgacga
     7321 ccgccgcgcc atcagccgcc agccgcagcg cctcagcccg gccgatcccg ccgcctgccc
     7381 cggtgacgat cgccactttg ccttgctggt ccaatgccac ctgaacgcat tccttctctg
     7441 gtttccgcct gccggatcga caaagcgcac tggccgtaaa ttcgcaagac aaatgtcaag
     7501 ccaactggcc tgatccgggt tcagtttcgc ccaggaccac gccgacgcag caacagcagc
     7561 aggggcagca tggcgaagtt gagcagggcc atgtcgcgga aggtggcggt gtaagccacc
     7621 atggtggcct gcctggtgac ctcggcgttg acggtggaca ggacgggggc agccgaggtc
     7681 gtgcccaagg cctcccgcat gaccccggg
//
LOCUS       DATNBV010000021         4532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3206856,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000021 DATNBV010000000
VERSION     DATNBV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3206856"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VN627_00545"
     CDS             <1..408
                     /locus_tag="VN627_00545"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HXD01771.1"
                     /translation="QTSEGAAAPETKPGLTLSGGKLVLPAVSGNPGAAYFTLTNGSPR
                     PVTIAAVDVAGAGMAMMHETRDVGGHSEMTMLESPTLAPGAALAFARGGKHVMVMDLP
                     GGLKPGGTIELTLTFAGGDKLSAPLAIVGPGGE"
     gene            405..899
                     /locus_tag="VN627_00550"
     CDS             405..899
                     /locus_tag="VN627_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vgr related protein"
                     /protein_id="HXD01772.1"
                     /translation="MNALPPGGERPLTPGETALVREVFGRAVDPRPVRIRRRRWFPLQ
                     PRETVMAPCGHLHFHPHGSRYSDDFSGEGPALRGLFVHELTHVWQAQTRGKLWLPLMR
                     HPFCRYDYVVREGWPLARYGIEQQAEIVRHAHHAARGEPVPPDVPADWLTARLADVQH
                     RTSY"
     gene            complement(878..1117)
                     /locus_tag="VN627_00555"
     CDS             complement(878..1117)
                     /locus_tag="VN627_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01773.1"
                     /translation="MITRFMLPVAAAGALALGGCATNYGVEGAAAGGLGGALIGGGTG
                     AVIGAAAGAAVGSLIKKDGRCYRVSRNGSQYEVRC"
     gene            complement(1200..1772)
                     /gene="grpE"
                     /locus_tag="VN627_00560"
     CDS             complement(1200..1772)
                     /gene="grpE"
                     /locus_tag="VN627_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXD01774.1"
                     /translation="MTDETTAEAGQDGAENENVLDLGGAEVSEQADAGGAELAALREE
                     LETAKQEVLYAKAETQNVRRRMEKEVADSRAYAATSFARDVLSVSDNLARAIESIPAD
                     LREDDRFRNLVAGIEATGREIDKVFASHGISRIAAMGLPLDPNQHQAMLEIPSADVEP
                     GTVLQELQAGYMIKDRLLRPAMVAVAKKPD"
     gene            complement(1791..2852)
                     /gene="hrcA"
                     /locus_tag="VN627_00565"
     CDS             complement(1791..2852)
                     /gene="hrcA"
                     /locus_tag="VN627_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HXD01775.1"
                     /translation="MASLPINELNARAREIFRLAVEGYLASGQPVGSKALAGAGSVNL
                     SPASIRSVLHDLEMLGLLAAPHTSAGRMPTELGLRLFVDGMMQVAEPTADERAAIERR
                     LAEPGPIEQALAATSAILSELSAAAGVVMVPRREARLSQLQLVPLGADQALAVLVSED
                     GAIENRVIPLPPGTPATSLQQASNYLTARLAGRTLGDAIAAIRGEIDAGRSALDAASR
                     DLVERGLALWSQDAQARPVLIVRGQANLLDETALGDLERVRSLLDDLENKQSVAALLD
                     AAREAEATRIFIGAENRLFALSGSAVIASPYRDREGRVVGVVGVIGPTRLNYARVVPM
                     VDFTARALSAMGGSSKLIG"
     gene            2948..3664
                     /gene="rph"
                     /locus_tag="VN627_00570"
     CDS             2948..3664
                     /gene="rph"
                     /locus_tag="VN627_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HXD01776.1"
                     /translation="MRPSGRAPDEMRALSFEPHFTKHAEGSVLVSFGHTKVICTASVE
                     ERVPPFMRGKGEGWVTAEYSMLPRATHTRGSREAAKGKQSGRTQEIQRLIGRALRSVV
                     DFKKLGERQITLDCDVIQADGGTRTAAISGAWVALRIAIDKLMAQGLVKEDPLPARVA
                     AVSCGIFKGTPVLDLDYDEDSSADADANFVLIEGGHIAEVQATAEGATYDEEGLLRLL
                     RLARIGCDNIFAAQAKAVGR"
     gene            3768..4388
                     /gene="rdgB"
                     /locus_tag="VN627_00575"
     CDS             3768..4388
                     /gene="rdgB"
                     /locus_tag="VN627_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HXD01777.1"
                     /translation="MPKLTGQLVIATHNAGKLREISALLAPYGVECLSAGELGLAEPA
                     ETGTTFAENALIKAHAAAKGANLPALADDSGLCVHALGNRPGVYTADWAERQWFEGEK
                     GRDWYMAMGKIEGLLCELGPDADRSAHFACTLALAWPDGTEAIYEGTVQGSLTWPPRG
                     AKGFGYDPVFIARGMEQSFAEIAPEAKHQISHRADAFAKLVAAQFG"
BASE COUNT          733 a         1721 c         1385 g          693 t
ORIGIN      
        1 cagaccagcg agggcgctgc cgccccggaa accaagccgg gcctcaccct gagcggcggc
       61 aagctggtcc tgccggctgt cagcggcaat cccggcgcgg cctatttcac gctcaccaac
      121 ggctcgccca ggcccgtcac catcgccgcg gtcgacgtgg ccggcgccgg catggcgatg
      181 atgcatgaaa ccagggacgt cggcggccac tccgagatga ccatgctgga aagtcccacc
      241 ctcgcccccg gcgccgcgct ggcattcgcc aggggcggca agcacgtcat ggtgatggac
      301 ctccccggcg gcctcaagcc cggcggaacg atcgagctca ccctcacctt cgccggtggc
      361 gacaagctct cggccccgct cgccatcgtc ggtccgggcg gcgagtgaac gcgctcccgc
      421 ccggcggcga gcgcccgctc accccgggcg aaacggcgct ggtgcgcgaa gtcttcggcc
      481 gcgcggttga cccgcggccg gtccgcatcc gtcgccgccg ctggtttccc ttgcagccgc
      541 gcgagacggt catggccccc tgcggccatc tccacttcca cccgcacggc agccgctatt
      601 ccgatgattt ttccggcgaa ggccctgcac tgcgcggcct gttcgtccac gagttgaccc
      661 acgtgtggca ggcccagacc cgcggcaagc tatggctccc gctgatgcgc cacccgttct
      721 gccgttacga ctatgtcgtg agggaaggct ggccgctcgc ccgctacggg atcgagcaac
      781 aggccgagat cgtgcgccat gcccaccacg cggcgcgcgg ggaaccggtc ccgccggacg
      841 tgccggccga ctggctcacc gcgcgactgg cggacgttca gcaccgcacc tcatactgac
      901 tgccgttgcg gctcacccgg tagcagcgcc catccttctt gatcagcgaa cccaccgccg
      961 cgcccgccgc cgcgccaatc accgcgccag tcccgccgcc gatcaaggcg ccgccgaggc
     1021 cgcccgccgc ggcgccttcg acgccgtaat tggtcgcaca accacccagc gccagcgcgc
     1081 cggcggcagc aaccggaagc ataaagcgtg taatcatgat ccgttctcca ttgccctgtt
     1141 cccaagggga atgaacaacg gagcgcgcca gttcccgcgc gccggccggc ggcctccgct
     1201 cagtccggct tcttggccac ggccaccatg gcgggccgca gcaggcggtc cttgatcatg
     1261 tagccggcct gcagctcctg cagcacggtg ccaggctcga catcggccga ggggatctcc
     1321 agcatggcct ggtgctggtt cggatccagc ggcaggccca tcgcggcgat gcggctgatg
     1381 ccgtggctgg caaaaacctt gtcgatctcg cggcccgtcg cctcgatccc ggccaccagg
     1441 ttcctgaagc ggtcatcctc gcgcaggtcg gcgggaatcg attcgatcgc ccgggcgagg
     1501 ttgtcggaaa cggaaagcac gtcgcgggca aaggaggtcg cggcataggc gcggctgtcg
     1561 gccacttcct tttccatccg ccgccgcacg ttctgcgttt ccgccttggc gtagagcacc
     1621 tcctgcttcg ccgtctccag ctcctcgcgc agcgcggcca gttcggcccc gccggcatcg
     1681 gcctgctcgc tcacctcggc cccgccgaga tcgagcacgt tctcgttttc cgcgccgtct
     1741 tgtccggcct ctgcggtcgt ttcgtcagtc atgattctcg tgtgttccag ctatccgata
     1801 agcttgcttg agccgcccat ggcactgagc gcccgcgcgg tgaagtccac catggggacc
     1861 acccgtgcat aattcaaccg cgtcgggccg ataaccccca ccacgccgac cacccgtccc
     1921 tcccggtcgc gatagggcga ggcgatcacc gccgaacccg aaagcgcgaa caggcggttc
     1981 tccgcgccga tgaaaatgcg cgtcgcctcg gcctcgcgcg ccgcatccag cagggcggcg
     2041 acggactgct tgttttccag atcgtccagc agcgagcgca cccgttccag gtcgcccagc
     2101 gcggtttcgt ccagcaggtt ggcctgcccc cgcacgatca gcacgggccg cgcctgggca
     2161 tcctggctcc acagcgccag cccgcgttca acgagatcgc ggctcgccgc atccagcgcc
     2221 gaacgccccg cgtcgatttc gcccctgata gccgcaatgg cgtcgcccag cgtgcgcccg
     2281 gccagccgcg cggtcagata gttggaagcc tgttgcaggc tcgttgccgg tgtccccgga
     2341 ggcagcggga tcacgcggtt ctcgattgct ccgtcctcgc tcaccagcac ggccagcgcc
     2401 tgatccgcgc caagcggcac cagctgcaat tgcgagaggc gcgcttcccg gcgcggcacc
     2461 atgaccacgc ccgccgccgc cgaaagctcg gaaagaatcg cgctggtcgc cgccagcgcc
     2521 tgttcgatcg gcccgggctc ggccagccgc cgctcgatcg ccgcgcgctc gtccgccgtc
     2581 ggctcggcca cctgcatcat cccgtcgacg aacagcctca ggccgagctc tgtcggcatc
     2641 cgccccgcgc tggtatgcgg cgcggccagc aggcccagca tctccaggtc atgcagcacc
     2701 gaacggatgg aggccggcga caggttcacc gatcccgccc ccgccagcgc cttcgagccc
     2761 accggctggc ccgaggcaag atagccctcc accgcgaggc ggaagatctc gcgggcgcgg
     2821 gcgttcagtt cgttgatcgg aaggctggcc atgttttctt cgccctaatg taagcctcgc
     2881 tcttgcagcc tcaagtccga agcgccaaac gccccccagc tttgcacaca tcagacaagg
     2941 aatttccatg cgtccctccg gccgtgcccc cgacgaaatg cgcgcgctca gcttcgagcc
     3001 tcacttcacc aagcacgccg aaggctcggt gctggtctcc ttcggccaca ccaaggtgat
     3061 ctgcacagca tcggttgaag agcgcgtgcc cccgttcatg cgcggcaagg gcgaaggctg
     3121 ggtcacggcg gaatactcga tgctcccccg cgccacccac acccgcggca gccgcgaggc
     3181 ggccaagggc aagcagtcgg gccgtaccca ggaaatccag cgcctgatcg gccgcgccct
     3241 gcgttccgtc gtggacttca agaagctcgg cgaacgccag atcaccctcg attgcgacgt
     3301 catccaggcc gatggcggga cccgcaccgc cgccatctcc ggcgcctggg ttgccctcag
     3361 gatcgccatc gacaagctga tggcccaggg cctcgtcaag gaagacccgc tcccggcccg
     3421 cgtcgccgcg gtctcctgcg gcatcttcaa gggcaccccg gtgcttgacc ttgactatga
     3481 tgaggacagc tcggccgatg ccgatgccaa cttcgtcctg atcgaaggcg gccacattgc
     3541 cgaagtccag gctaccgccg aaggcgcaac ctacgacgag gaaggcctcc tgcgcctcct
     3601 gcgcctcgcc cgcatcggct gcgacaacat cttcgccgcc caggccaagg ccgtcgggcg
     3661 ctgacaccca gatcctcccc gttttcgtcc gcagggcgca aatggggagg tggcagtcgc
     3721 cgcaggcggc tgacggaggg gcaaaagctc ccaaacggaa cccgaccatg cccaaactca
     3781 ccggccaact cgtcatcgcc acccacaacg cgggcaagct gcgcgaaatt tccgcgctgc
     3841 tcgcccccta tggcgtggaa tgcctgtccg ccggcgagct cggcctcgcc gaacccgccg
     3901 aaaccggcac caccttcgcc gagaacgccc tgatcaaagc ccatgccgcc gccaagggcg
     3961 cgaacctccc cgcgctggct gacgattcgg gcctctgcgt ccacgcgctc ggcaatcgcc
     4021 ccggcgtcta cacggcggac tgggccgaac ggcaatggtt cgagggcgaa aagggccgcg
     4081 actggtacat ggccatgggc aagatcgaag ggctgctctg tgaactcggc ccggatgccg
     4141 accgctccgc ccacttcgcc tgcaccctcg ccctcgcctg gcccgacggc accgaggcga
     4201 tatacgaagg cacggtgcag ggcagcctca cctggccccc gcgcggcgcc aagggcttcg
     4261 gctacgatcc ggtgttcatc gccaggggca tggaacagag ctttgccgag attgcccccg
     4321 aggcaaaaca ccagatcagc caccgggcgg atgccttcgc caaactggtc gcagcccagt
     4381 tcggctgacg gcccccttca ccactctttc ctacaccgtc atcctgaact tgtttcagga
     4441 tccacaggcc gagctatccc ccatcgcggc ggtagaaccg gaagcgccgt gcctgcggcg
     4501 cagttggcgc catggatgct gaatcaggtt ca
//
LOCUS       DATNBV010000022         3720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4492451,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000022 DATNBV010000000
VERSION     DATNBV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4492451"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VN627_00580"
     CDS             complement(<1..167)
                     /locus_tag="VN627_00580"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337893.1"
                     /note="catalyzes the formation of 5-aminolevulinate from
                     succinyl-CoA and glycine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HXD01778.1"
                     /translation="MNYEHIFNQAIERLHSEGRYRVFIDILRNKGAYPNARCFAGHNG
                     PEPITVWCSND"
     gene            complement(273..1190)
                     /locus_tag="VN627_00585"
     CDS             complement(273..1190)
                     /locus_tag="VN627_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01779.1"
                     /translation="MTHRLNLFLLCLAVAVALPYYWLVLHNPSRNLAPEPVHMADLRR
                     LADGLPGPRPASVTVTLVGWDRTPGTLLAAGAGLKRRMYSVVSFRLDVPGQGPIVIDT
                     GTTAPLARKEGVEGFRPLRQQQLYSDMRAASHIVPTDESPETLAGLAALAQASNSAMA
                     LTRARLNPAQLPAATRAAGLPWPDRLTLGAAVRPGPPQVIAPGVVVIPTAAPTPGSQM
                     VYAHLADGREYLFAGPVAPYPVNFLDLRTRSNLLDLLEGHQDRVGAMRWLITLRQWQK
                     EAPRLYVVPGHDLMALTDKDAPSGIVYRE"
     gene            complement(1201..2049)
                     /gene="murI"
                     /locus_tag="VN627_00590"
     CDS             complement(1201..2049)
                     /gene="murI"
                     /locus_tag="VN627_00590"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HXD01780.1"
                     /translation="MVQSAAQPSPAEVPEIDPAAPVLLFDSGVGGLSVLDEVRALLPQ
                     APVIYAADTAGLPYGSKSEAEIAARVSGLLGRMTERLHPRLVCIACNTASTIALAAVR
                     EVLEVPIVGTVPAIKPAAACTQTGVIGLLGTAATIRQAYVDRLEAEFAQGKTLLRHAA
                     PELVGAAEAHLRGEAVDPAVYARAAEGLRAQPGGERIDTVVLACTHFPLVTDKLAEAF
                     GPGVRFVDGAAGIARRIAHLTQGQPFARREPDLALFTARLGEAAPWDGLLPALAARGL
                     VRTAVF"
     gene            2133..2729
                     /gene="plsY"
                     /locus_tag="VN627_00595"
     CDS             2133..2729
                     /gene="plsY"
                     /locus_tag="VN627_00595"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HXD01781.1"
                     /translation="MDGNSTLLVLAAGYLLGSIPFGLILTRLTGAGDLRSIGSGNIGA
                     TNVLRTGHKGLAAATLLFDLAKGAVAVVLARHFAGDGAAALGGAAAVLGHCFPVWLRF
                     KGGKGVATLMGVALGLAWPIGLAYAVVWLGMLAITRISSLGGMSAAVVAPFAALALGM
                     RSEAAVLAGLTALVLWLHRANIGRLREGTEPRVGSKGK"
     gene            2735..>3720
                     /gene="dprA"
                     /locus_tag="VN627_00600"
     CDS             2735..>3720
                     /gene="dprA"
                     /locus_tag="VN627_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXD01782.1"
                     /translation="MSGGTAALDQTEAFARIRLLRSPNVGPVSYRQLVARFGSASAAL
                     DALPDLVGRRGAAYRPASEARVADEIAAVRKAGARFLFEDSADYPALLRECEGAPPIL
                     VARGHAELAIRPAVAIVGARNASAAAVRLAREFGGALAEAGYAVVSGLARGIDGAAHQ
                     GALARAMDGGGTIGVIASGIDINYPPEHAALQEQIAQEGLLLAEQPAGTEPLARHFPA
                     RNRIIAGIAAGTLVVEAAWKSGSLITARLAGEMGREVMAIPGSPLDSRSHGCNQLIRD
                     GAVLCQRPEDVIELLEGFTGQPRSTFREAAPAFGAASTSEPAEPADVAGLLTT"
BASE COUNT          564 a         1299 c         1296 g          561 t
ORIGIN      
        1 tagtcgttcg agcaccacac cgttatcggc tcgggaccgt tatgcccggc aaagcaccgc
       61 gcattgggat aggcgccctt gttgcgcagg atgtcgatga acacccggta gcggccttcg
      121 gaatggagcc gctcgatcgc ctggttgaaa atgtgctcgt agttcacttc agccaaagtc
      181 ctcgaggccg gcccagcagg cgcgctgtct gccgcgccaa tataggaagg cgcggtcaat
      241 ttccagcgcg tttctgcgca agggcgattg cgttattcgc gatagacaat gccgctggga
      301 gcatccttgt cggtcagcgc catgaggtcg tgcccgggga cgacgtagag ccggggcgct
      361 tccttttgcc actggcgcaa ggtgatcagc caccgcattg cgccgacgcg gtcctggtgg
      421 ccttcgagca ggtcgaggag gttggaccga gtgcgcagat cgaggaaatt caccggatag
      481 ggggcaaccg ggccagcaaa caggtattcg cggccatcgg cgagatgcgc gtagaccatc
      541 tggctgccgg gcgtcggcgc agcggtcgga atcacgacga cccccggtgc gatgacttgt
      601 ggcgggccgg ggcgaaccgc cgcgccgagc gtgagccgat caggccaggg cagcccggca
      661 gcgcgagtcg cggcgggtag ctgggcgggg ttgagccggg cgcgggtaag cgccatcgcg
      721 gaattcgacg cctgcgcaag cgcagcaagg ccggccagcg tttcaggact ctcgtcggtt
      781 gggactatgt ggctggccgc gcgcatgtcg gaatagagct gctgctgacg aagcgggcgg
      841 aacccttcca ctccctcctt gcgggcgagc ggcgccgttg tcccggtatc gatcacgatc
      901 gggccttgcc cgggcacgtc caggcgaaac gacacgacgc tgtacatgcg ccgcttgagc
      961 ccggccccgg ccgccagcaa ggttcccggg gtgcggtccc agccgacgag cgttacggtg
     1021 accgaggccg ggcgggggcc gggaagccca tccgccaggc ggcgcagatc ggccatgtgg
     1081 accggttcag gcgcaaggtt gcgcgacggg ttgtgcagga ccagccagta atagggcagc
     1141 gccacggcaa cggccaggca gagcaggaac aggttcagcc gatgcgtcat tccgcagcga
     1201 tcagaacacg gccgttcgca ccagcccgcg cgcggcgagg gcggggagca gcccgtccca
     1261 cggcgccgct tcgccgaggc gggcggtaaa cagggccaaa tcgggttcgc gccgggcaaa
     1321 cggctggccc tgtgtcaggt gggcgatccg ccgcgcgatg cctgctgcgc cgtcgacgaa
     1381 ccgcacaccc ggtccgaagg cctcggccag cttgtcggtg accagcggga aatgggtgca
     1441 ggccagcaca acggtatcga tccgctcgcc gccgggctgg gcgcgcaggc cctcggcggc
     1501 gcgggcatag accgccgggt ccaccgcctc gccgcgcagg tgcgcctcgg ccgcgccgac
     1561 cagctcgggc gcggcatggc gcagcagcgt cttgccttgg gcgaactcgg cctccagccg
     1621 gtcgacatag gcctggcgga tcgtcgccgc cgtgccgagc aggccgatca cgcctgtctg
     1681 cgtgcacgcg gcggcaggct tgatcgccgg aaccgtgccg acgatcggca cttccaacac
     1741 ttcgcgcacg gcggccagtg caatcgtgct cgccgtgttg caggcaatgc agaccagtcg
     1801 cggatggagc cgctcggtca ttcggcccag caggcccgac acccgcgcgg caatctccgc
     1861 ctcgctcttg ctgccatagg gcaggccggc cgtatccgcg gcatagatca ctggcgcctg
     1921 cgggagcagg gcgcgcacct cgtccagcac ggaaagcccg cctacgcccg aatcgaacag
     1981 cagcacgggt gccgccggat cgatctctgg cacttctgca gggctaggtt gggcggcgga
     2041 ttggaccata ccgggggtat ccctaaattt cctctgcccg attgcaagga ccgccgctaa
     2101 agcagggcca ttatccgggg gaattcgatc gaatggacgg caattcaacc ttgctggtgc
     2161 tggctgcggg ctacctcctc ggctccatcc cgtttggcct gatcctcacc cggctcaccg
     2221 gcgcgggcga tctgcggtcg atcggttcgg ggaatatcgg cgccaccaac gtgctgcgca
     2281 ccgggcacaa ggggctggca gcggccaccc tgctgttcga tctcgccaag ggcgcggtgg
     2341 ccgtggtgct ggcacggcac ttcgctgggg atggcgcagc cgcgctcggc ggtgcggcgg
     2401 ccgtgttggg gcattgcttc ccggtctggc tgcggttcaa ggggggcaag ggcgtcgcca
     2461 ccctgatggg cgtggcgctc ggcctcgcat ggccgatcgg ccttgcctat gccgtcgtct
     2521 ggctcggcat gctggcgatc acccgcatct cgtcgctcgg cgggatgagc gcggccgtcg
     2581 tcgccccctt tgccgccctg gccctgggca tgcgctccga agcagcggtg ctcgcaggcc
     2641 tcaccgcgct ggtcctctgg ctccaccgcg ccaacattgg ccgcctgcgg gaggggactg
     2701 agccgcgggt cggcagcaag ggcaagtagg tccaatgtcg gggggcaccg ccgcgctgga
     2761 ccagacggag gccttcgccc gcatccgcct tctgcgctcg cccaacgtcg gcccggtcag
     2821 ctatcgccag ctggtcgccc ggttcggttc ggccagcgcg gccctcgatg ccctgccgga
     2881 tctcgtcggc cgccgcggcg ccgcctaccg tcccgcgagc gaagcccgcg tggcggacga
     2941 gatcgccgcg gtccgcaagg ccggcgcccg cttcctgttc gaggacagtg cggactatcc
     3001 cgccctcctg cgcgaatgtg aaggcgctcc gccgatcctc gtcgcccgag gccacgccga
     3061 actcgccatc cgccccgcag tcgcgatcgt cggcgcgcgc aatgcctcgg cggccgccgt
     3121 gcggctcgcg cgcgaattcg gcggcgcgct cgccgaagcg ggctatgcgg tcgtctccgg
     3181 cctggcgcgc gggatcgacg gggcagccca ccagggcgcg ctggcccggg cgatggacgg
     3241 cggcggcacc atcggcgtga tcgccagcgg catcgacatc aactatcccc ccgaacatgc
     3301 cgccctgcag gaacagatcg cgcaagaagg cctcctcctc gccgaacaac ccgccggtac
     3361 cgagccgctc gcccgccact tccccgcgcg caaccgcatc atcgccggca tcgcggcggg
     3421 cacgctggtg gtggaagccg cctggaaatc gggctcactc atcactgcgc gccttgccgg
     3481 cgaaatgggc cgcgaagtca tggccatccc cggctccccg ctcgacagcc gctcgcacgg
     3541 ctgcaaccag ctgatccgcg acggcgctgt cctctgccag cgcccggaag acgtgatcga
     3601 attgctcgaa ggcttcacgg gccagccgcg atcgaccttc cgcgaggcag cccctgcttt
     3661 cggtgccgcc agcacatcgg aaccggccga accggctgac gtggcaggcc tgctcaccac
//
LOCUS       DATNBV010000023         5540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_975277,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000023 DATNBV010000000
VERSION     DATNBV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975277"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /gene="atpA"
                     /locus_tag="VN627_00605"
     CDS             complement(<1..1524)
                     /gene="atpA"
                     /locus_tag="VN627_00605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HXD01783.1"
                     /translation="MEIRAAEISKVIKDQIASFGTEAQVSEVGTVLSVGDGIARIHGL
                     DKVQAGEMVEFANGVKGMALNLEADNVGVVIFGSDAEIKEGDTVKRTETIVDVPVGKG
                     LLGRVVDALGNPIDGKGPIVDAVRQRVEVKAPGIIPRKSVHEPVQTGLKAIDALVPVG
                     RGQRELIIGDRQTGKTAIAIDTFINQKGANAGTDESKKLYCIYVAVGQKRSTVAQIVR
                     QLEENGAMEYSIVVAATASEPAPLQYLAPYTGATMGEFFRDNGMHAVIVYDDLSKQAV
                     AYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAMGGGSLTALPVIETQAGDV
                     SAYIPTNVISITDGQIFLETGLFYQGVRPAINVGLSVSRVGGAAQTKAMKKVAGSIKL
                     ELAQYREMAAFAQFGSDLDASTQKLLNRGARLTELLKQKQFSPLAFEEQTVSIFAGTN
                     GYLDNIAVSQVVEYEAEMLSFLRDKHADILAGIRESKDFSDDSKSKLKAALDAFGKQF
                     "
     gene            complement(1538..2092)
                     /locus_tag="VN627_00610"
     CDS             complement(1538..2092)
                     /locus_tag="VN627_00610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HXD01784.1"
                     /translation="MENSSGITASLQGRYAAALFELAREQNLVSAVEGDLDNLGQALV
                     ASPDFSALIRNPKVSREAAAQAMDAVAGVLGLSPLTKNFLGVLAGNRRLSALPEIVRA
                     FAAIAAAERGEVTAEVTSAHPLTDDQLGALAAKLQAREGRTVKLKTSVDPDLLGGLVV
                     RIGSTQIDSSIRTRLNSLAQAMKG"
     gene            complement(2357..3919)
                     /locus_tag="VN627_00615"
     CDS             complement(2357..3919)
                     /locus_tag="VN627_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXD01785.1"
                     /translation="MKRILALLALLLAVPLALLPAPATADPSDIAAASRSVVRVVLIS
                     RTAAGVALIGHGSGFAVAPDLVVTNAHVVAPLAEDPNMRAGIVPSQGKSGYFAKVVAF
                     SPRNDLALLRLVEKGTLVPATLFTGAVTDGEDVYAVGYPGNVDQAQGLNPADLMSPVA
                     PVKTRGAVSAGRTSKGFETILHTAPIGSGNSGGPLLDACGRVIGANSFGTASETSADS
                     EFYFAISMREIMRFLTNAGINVRSTGEPCRSLADLDAAERERQAGAQALSEQQQRVAE
                     AKQSAALQAATRRAEMEVIASRENHLALAGLALLLAMASGAAAIWLGPQVRRRRERKG
                     AIVAAAVLVLLVPVAWFTRPSIAGIDDRAHDILAEASESAAPQASTTPSAPAEAAYTC
                     VFNPQRSRVTVSETADVPLNWSADGCVNGRTQYGLGAEGWSKVLAPGAEDSVTIASFD
                     PSTRTYRQDRYLLDMDTMAKVRAERGKNPAPACGAGEAEARKLGDAQAALRALLPAQP
                     NERMIYDCHPAQ"
     gene            4017..5066
                     /gene="ada"
                     /locus_tag="VN627_00620"
     CDS             4017..5066
                     /gene="ada"
                     /locus_tag="VN627_00620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HXD01786.1"
                     /translation="MTIAMSVDPINPDQAWAAVLARDRAADGRFVTGVLSTGIYCRPS
                     CAARHPARANVRFFADGRAARAAGLRPCRRCRPDEVARDEQAVLAAVAAIKAAGRGLS
                     LADLAAASGYSPGHFQRLFLRHTGLSPAAYARALRAGRAADALTEEHRVTDAVYAAGY
                     GAPSRFYEEAGRRLGMPPSAWSRGGEGVTIRWAVAKTSFGSMLVAATDRGLCRLAFGE
                     DEGDLSRRFPQARLEPGGAPFGALLAEVVAAVEEPGKGGNIPLDVRGTAFQERVWQAL
                     RAIPPGETRSYAQIAAAAGKPSAVRAAGSANGANPVAVLIPCHRVVRSDGSLGGYAWG
                     EEIKRELLRRERKEP"
     gene            complement(5079..>5540)
                     /gene="mazG"
                     /locus_tag="VN627_00625"
     CDS             complement(5079..>5540)
                     /gene="mazG"
                     /locus_tag="VN627_00625"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828544.1"
                     /note="functions in degradation of stringent response
                     intracellular messenger ppGpp; in Escherichia coli this
                     gene is co-transcribed with the toxin/antitoxin genes
                     mazEF; activity of MazG is inhibited by MazEF in vitro;
                     ppGpp inhibits mazEF expression; MazG thus works in
                     limiting the toxic activity of the MazF toxin induced
                     during starvation; MazG also interacts with the GTPase
                     protein Era; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HXD01787.1"
                     /translation="ASHEANREVRWEKLKAAERAAKGATSALDGVALALPALMRAEKL
                     QKRAARVGFDWDDFNGPADKVREELDELAEAGSDEERFAEAGDLLFAAVNAVRALDIA
                     PEEALRQANDKFERRFKGMERIAEAQGEVFAELPIGRQEALWQQVKAGERA"
BASE COUNT          812 a         1866 c         1929 g          933 t
ORIGIN      
        1 gaactgcttg ccgaacgcgt cgagcgcagc cttcagcttg ctcttcgaat cgtcggagaa
       61 gtccttggat tcgcggatgc cggcgagaat gtcggcatgc ttgtcgcgca ggaacgaaag
      121 catctcggct tcgtattcga cgacctggct gaccgcgatg ttatcgaggt agccatttgt
      181 gccggcaaag atcgagacgg tctgttcttc gaaggcgagc gggctgaact gcttctgctt
      241 gagcagttcg gtcaggcgcg cgccgcggtt cagcagcttc tgcgtcgaag cgtcgaggtc
      301 cgagccgaac tgggcgaaag ccgccatttc gcggtactgc gcgagttcca gcttgatcga
      361 gccggcaacc ttcttcatcg ccttggtctg ggccgcaccg ccgacgcgcg acaccgagag
      421 gccgacgttg atggccgggc gcacgccctg gtagaacagg ccggtttcaa ggaagatctg
      481 gccgtcggtg atcgagatca cgttggtcgg aatgtaggcc gaaacgtcgc cggcctgggt
      541 ctcgatgacc ggcagcgcgg tcagcgaacc gccgcccatg gcgtcgttca tcttcgcggc
      601 gcgctcgagc aggcggctgt ggagatagaa cacgtcgccc gggtaggctt cgcggcccgg
      661 cgggcggcgc agcagcagcg acatctggcg gtaggcgacg gcctgcttgg aaaggtcgtc
      721 gtacacgatc acggcgtgca tgccgttgtc gcggaagaac tcgcccatgg tcgcaccggt
      781 gtagggcgcg aggtactgga gcggagccgg ctcggaagcg gtcgcggcca cgacgatgga
      841 gtattccatc gcgccgttct cttcgagctg gcggacgatc tgcgccacgg tcgaacgctt
      901 ctggccgacg gcgacataga tgcagtagag cttcttgctc tcgtccgtgc cggcgttggc
      961 gcccttctgg ttgatgaagg tgtcgatcgc gatggcggtc ttgccggtct ggcggtcacc
     1021 gatgatcagc tcgcgctggc cgcggccgac ggggacgagg gcgtcgatcg ccttcaggcc
     1081 ggtctgcacg ggttcgtgca ccgacttgcg ggggatgatg cccggcgcct tgacttccac
     1141 gcgctggcgc acggcgtcga cgatcgggcc cttgccgtcg atcggattgc cgagcgcgtc
     1201 gaccacgcgg ccgagcaggc ccttgccgac gggcacgtcg acgatggtct cggtgcgctt
     1261 gacggtgtcg ccttccttga tctcggcgtc cgagccgaag atcacgacgc cgacgttgtc
     1321 ggcctcgagg ttgagggcca tgcccttcac gccattggcg aattcgacca tttcgccggc
     1381 ctggaccttg tcgaggccgt ggatgcgggc gatgccgtca ccgaccgaaa gcacggtgcc
     1441 gacttccgag acctgggcct cggtgccgaa gctggcgatc tggtccttga tgaccttcga
     1501 gatttctgcg gcgcggattt ccattgtttc gcctttctta gcccttcatg gcctgggcaa
     1561 gggaattgag acgggtgcgg atcgagctgt cgatctgggt ggacccgatg cggacgacga
     1621 ggccgcccag caggtccgga tcgacgctcg tcttgagctt gacggtgcgg ccttcgcggg
     1681 cctgcagctt ggcggcgagg gccccaagct ggtcgtcggt cagcgggtgg gcactggtga
     1741 cttcggcggt aacttcaccg cgctcggccg cggcgatcgc ggcgaaggcg cggacgatct
     1801 cgggcagggc cgacaggcgg cggttgccgg cgaggacgcc gaggaagttc ttggtgagcg
     1861 gcgagagtcc gagcacgccg gcaaccgcgt ccatcgcctg ggcggcggct tcgcggctga
     1921 ccttggggtt gcggatcagg gcgctgaaat cgggcgaggc gacgagcgcc tggccgagat
     1981 tgtcgagatc gccttcgacc gcggagacga ggttctgctc gcgcgcgagc tcaaagagcg
     2041 ccgcggcgta gcgcccctgc agactggcag tgatgccgct ggaattctcc acgcgcgtcg
     2101 tttccttctt tgtccgggtg gctgaacgaa ccgggtgatc gccagggcgc ggggcggcgc
     2161 gaaaggcagc ccccctgtcg acgccgccgc gcctagcaat gaatgttcgc agatgcaagg
     2221 catcgggagc acaaggcgaa aacatcggaa ttccgcacgt tttccagccc cggcgccgcg
     2281 agtcgctgcg agatttgccc cgatttgcgc attcgtatcc gccccggcgg ggcgagggga
     2341 gattcgagcc ggtgctctat tgcgccggat ggcagtcgta gatcatccgc tcgttgggct
     2401 gggccggcag cagggcgcgc agcgccgcct gggcatcgcc gagcttgcgc gcctcggcct
     2461 cgcccgcgcc gcaggctggc gccgggttct tgccacgctc cgcgcgcacc ttggccatgg
     2521 tgtccatgtc gagcaggtag cggtcctgcc gataggtcct ggtggaggga tcgaaactgg
     2581 cgatggtcac cgaatcctcc gcccccgggg cgagcacctt cgaccagccc tcggccccca
     2641 gcccatactg cgtgcgcccg ttgacgcagc cgtccgcgct ccagttcagc ggcacgtcgg
     2701 ccgtctccga aacggtcacc cggctgcgct gcggattgaa cacgcaggta taggcagctt
     2761 ccgccggggc agagggtgtg gtgctcgcct ggggcgcggc ggattcgctc gcttcggcaa
     2821 ggatatcgtg cgcccggtcg tcgattcccg cgatcgaagg gcgggtgaac caggcaaccg
     2881 gaaccagcag gaccagcacg gcagcggcga cgatcgcccc cttgcgctcg cgccggcgcc
     2941 ggacttgcgg gcccagccag atcgccgcgg cgccgctcgc catcgccagc agcagggcaa
     3001 ggccggccag cgccaggtga ttctcgcgcg aggcgatcac ttccatctcg gcccggcggg
     3061 tcgccgcctg cagggccgcg ctctgcttcg cctcggcgac gcgttgctgc tgctccgaaa
     3121 gggcctgggc gccggcctgg cgctcacgct cggccgcatc gaggtcggcg aggctccggc
     3181 agggctctcc ggtcgatcgg acgttgatcc cggcattggt caggaagcgc atgatctcgc
     3241 gcatcgagat ggcgaagtag aactccgaat cggcgctcgt ttccgaagcc gtgccgaacg
     3301 aattggcccc gatcacccgc ccgcaggcat cgagcagggg cccgccggaa ttgcctgagc
     3361 cgatcggggc ggtgtgcagg atggtctcga accccttgct tgtccggccc gccgaaaccg
     3421 cgccgcgtgt cttaaccggg gcgaccgggc tcatcaggtc ggccgggttc aggccctggg
     3481 cctgatcgac attgccggga tagccgacgg catagacgtc ctcaccgtcg gtcaccgccc
     3541 cggtaaacag cgtggcaggg accagcgtgc ctttctcgac cagccgcagc agggcaaggt
     3601 cattgcgcgg cgaaaaggcc actaccttgg cgaaatagcc gctcttcccc tgggagggga
     3661 cgatccccgc gcgcatgttg ggatcttcgg ccagcggcgc gacgacgtgg gcattggtga
     3721 ccaccaggtc cggcgccacg gcaaacccgg agccatgccc gatcagcgcc acccccgcag
     3781 ccgtgcgcga gatcagcacc acgcggacga ccgagcgcga ggcggcggca atgtcgctgg
     3841 gatcggcggt tgccggcgct ggcagcaggg ccagcggcac ggcgagcagc agggctaaca
     3901 aggcaaggat ccgcttcacc cccgccgcct agcctatcgc atggtttgac cgcaagctgc
     3961 gggaggcgcg tcgtgcccga tcatggcaac acttccccca ggcaaggagc cctgtcatga
     4021 ccatcgcgat gagcgtcgac cccatcaatc ccgaccaggc ctgggcagcc gtgctggcgc
     4081 gtgatcgtgc ggccgatggc cggttcgtca ccggcgtcct ctcgaccggg atctattgcc
     4141 gccccagctg cgccgcgcgc catcctgccc gcgccaacgt ccgcttcttc gctgatgggc
     4201 gcgctgcccg cgctgcgggc ctgcgcccct gccgccgctg ccgcccggac gaggtcgcgc
     4261 gtgacgagca ggcggtgctg gccgccgtgg cagcgataaa ggcggcgggg agggggctgt
     4321 cccttgccga tctcgccgca gcctccggct attcccctgg tcacttccag cgtcttttcc
     4381 tgcgccatac cgggctctcg cccgccgcct atgcccgcgc cctgcgcgcc ggacgcgccg
     4441 ccgatgcgct gaccgaggag caccgcgtca ccgatgccgt ctatgccgcc ggctacggcg
     4501 cgccatcgcg cttctatgaa gaagccggca ggcgcctcgg catgccgcca tcggcatggt
     4561 cgcgcggcgg ggaaggggtg acgatccgct gggccgtcgc gaaaacgagc ttcggttcga
     4621 tgctggtggc cgcaaccgac cggggcctgt gccgcctggc attcggcgag gacgagggcg
     4681 atctttcccg gcgattccct caggcgcggt tggaacctgg cggcgccccg ttcggcgcgc
     4741 tgttggccga ggtcgtcgcc gcagtcgagg aaccggggaa ggggggcaac atcccgctcg
     4801 acgttcgcgg caccgccttt caggagcgcg tctggcaggc cctgcgcgcc attccgccgg
     4861 gcgaaacccg cagctatgcg cagatcgccg cggccgccgg taagccttcg gccgtgcgcg
     4921 cggccggttc ggccaatggc gccaatccgg tggcggtgct gattccctgc caccgggtgg
     4981 tgcggtcaga cggttcgctc ggcggttatg cgtggggtga ggagatcaag cgcgaactgc
     5041 tgcggcgcga gcgtaaagag ccgtagccgc cagggcaatc aggcccgctc gccggccttg
     5101 acctgctgcc aaagcgcctc ctgccgcccg atcggcagct ccgcaaagac ttcgccctgc
     5161 gcctcggcga tgcgttccat ccccttgaat ctgcgctcga atttgtcatt agcctggcgc
     5221 agggcctctt ccggggcgat gtccagcgcg cgcaccgcgt tgaccgccgc aaacagcagg
     5281 tcgccggcct cggcaaagcg ctcctcgtcc gaaccggcct cggccagttc gtcaagctcc
     5341 tcgcggacct tgtccgccgg cccattgaag tcgtcccagt cgaagcccac gcgcgccgcg
     5401 cgtttctgca gcttttccgc gcgcatgagc gccggcaggg ccaaggcgac accgtccagc
     5461 gccgatgtcg cgcccttggc cgcgcgctcg gcggccttga gcttttccca gcgaacctcg
     5521 cggtttgcct cgtgactggc
//
LOCUS       DATNBV010000024         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_21312,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000024 DATNBV010000000
VERSION     DATNBV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21312"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VN627_00630"
     CDS             <1..433
                     /locus_tag="VN627_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01788.1"
                     /translation="EPLLARLAGWDDRAGLTALVDGWEALLGEPPLGRATLETFAEGR
                     AGALATLAAQIGANPGDAAGAAHGWALAELAVDLPDPADRAAAARLLGERPRLAALPR
                     PLRPLAVLAGTTGRAARRGGAAELYRPGSFVTAVRLGLLGR"
     gene            534..1370
                     /locus_tag="VN627_00635"
     CDS             534..1370
                     /locus_tag="VN627_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD01789.1"
                     /translation="MHKIGTSERPDLALAVVVGAGGLGVAIARRMALSYRVLLVDVEG
                     ARVEAEAERLRGEGCDARAVACDITSAEAVADLAAGIAASGGFQAAVIVAGISPSGGN
                     FRQIAAVNLHGAALVVEALRPIARPGSAMVMISSLAAHLFTPSAEAAEILKKPADPGM
                     VEALERCLGPDGAIPTAAYGLSKWGMNLMARRQAPAWGERGARIVTLSPGLIATPMGA
                     REFAASEAKRELYKKSPILRECTMLEIADGVEFLASPRASFISGTDLRIDGGLAAAVL
                     DA"
     gene            1418..1960
                     /locus_tag="VN627_00640"
     CDS             1418..1960
                     /locus_tag="VN627_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01790.1"
                     /translation="MNRTVLGAFGALLLAAAGMFWWQGRAAIDPGKLPLVGAASLAGG
                     DLSLPTADGHGLNGPALPEASEATKEQRRFDRLDRNRDGRITRVEMLAPRVAAFRKLD
                     VDGNNLLTFEQWAVRGSNRFKAADANGDGQLDRMEFATTKPKPRAAPACKCAGPQRKT
                     PAPPAGGTGADDGDEADQPN"
     gene            complement(1979..>2725)
                     /locus_tag="VN627_00645"
     CDS             complement(1979..>2725)
                     /locus_tag="VN627_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXD01791.1"
                     /translation="IFSVLGGMAIATPALADEANWTGPYVGVIAGWNATRSDTAATLG
                     GTWSTEAAATRTAVIDGLAARQSNNNGNIGAILGYNYQTGGIILGLEAGASTIGGSNE
                     RVTGPISPVGIPALNFVFSNKIDPKSLISVKARLGAAVGSHTMIYATGGWAWVDANHS
                     ASITSNGNYKKLGELGKTHNGFIVGAGIEHKLDSHLSIRAQYDYTDQGDVTYATAYLP
                     GSAFTTPAYSETLSQDLRLHLLRVGVSYNF"
BASE COUNT          404 a          856 c          997 g          468 t
ORIGIN      
        1 tgagccactg ttggcccggc tggcgggatg ggacgatcgt gccgggctca cggcgctggt
       61 cgacggctgg gaagccctgc ttggcgagcc gccgcttggc cgggcgacac tcgagacatt
      121 tgccgaaggc cgggcgggag cgctggcgac attggcggcg cagatcggtg ccaaccccgg
      181 cgacgcagcg ggtgcggcgc atggctgggc gctggcggaa ctggccgtcg acctgccgga
      241 cccggccgac cgcgcggcgg cggcacggct gctgggcgag cggccccgct tggctgcctt
      301 gccccgcccc ctgcggccgc tggccgtcct agctggcaca acggggcggg cggcgcggcg
      361 tggcggggca gctgaactct atcgccccgg gtcgttcgta acggcggttc gcctggggct
      421 gctgggccgc tgatggcgtt cgagcgcgga tgtgcttgaa ttccgagagc cttgcgcgcc
      481 aggtattcgc tggcacaatt tctgaagcag aacggtaagg cggggacagc aggatgcaca
      541 agatcggaac cagcgagcgg ccggaccttg ccttggcggt cgtggttggc gcaggtgggc
      601 tgggcgtcgc gattgcccgg aggatggccc tgtcctatcg ggtgctgctg gtcgatgtcg
      661 agggcgcccg cgtcgaagcc gaggcggaac gactgcgggg ggaaggctgc gatgcccgcg
      721 cggtcgcctg cgacatcacg tcggcggagg cggttgccga ccttgctgcc gggatcgcgg
      781 caagcggtgg tttccaggcg gcggtgattg tggcggggat ttcaccttcg ggcggcaatt
      841 tccggcagat tgcggcggtg aacctgcacg gcgcagccct cgtggtcgaa gccctgcgcc
      901 cgattgcaag gcccggcagt gccatggtga tgatctcgtc gctggcggcc cacctgttca
      961 cgccctccgc cgaggccgcg gaaatcctga agaagcctgc cgatccgggc atggtcgagg
     1021 cgctggaacg ctgtcttggc cccgacggag ccattccgac cgcagcttac ggtctgtcga
     1081 aatggggcat gaacctgatg gcccggcggc aggcgcctgc ctggggcgag cgaggcgcgc
     1141 ggatcgtcac cctgtcgccc ggtctgatcg ctaccccgat gggcgcgcgc gaattcgcgg
     1201 cgagcgaggc caagcgggag ctttacaaga aatcgccgat cctgcgcgaa tgcaccatgc
     1261 tcgagattgc cgacggcgtg gagttccttg cctcgccgcg cgcctcgttc atctcgggaa
     1321 ccgacctgcg gattgatggc ggactggctg ccgcagtgct ggatgcctga cgggcactgc
     1381 gctagcctgc ctctagggga atctccgggg ggtagtgatg aatcgaaccg tgcttggcgc
     1441 tttcggcgcc ctcctgctgg cggcagcggg catgttctgg tggcagggcc gcgcggcgat
     1501 tgatccgggc aagctgccgc tggtcggcgc ggccagcctc gccggcggtg acctgtcgct
     1561 tcccacggcc gatgggcatg gcttgaatgg tccagcgctg cccgaggcga gcgaagcgac
     1621 gaaggagcag cggcgcttcg accgcctgga ccgcaatcgc gatggccgga tcacccgcgt
     1681 cgaaatgctg gcgccccgcg tcgccgcctt ccgcaagctc gacgtcgacg gcaacaacct
     1741 gctgactttc gagcaatggg ccgtgcgcgg gtccaaccgg ttcaaggcgg ccgatgcgaa
     1801 tggcgacggc cagctcgacc gtatggagtt tgccacgacc aagcccaagc cgcgtgcggc
     1861 tccggcctgc aaatgcgccg gaccgcaacg gaaaacgccg gctccccccg cgggcggtac
     1921 cggcgccgac gatggcgatg aagcggacca gccgaactga tccgcccatc ttgtcggatc
     1981 agaagttgta gctgactccc acgcgaagca ggtgcaggcg caggtcctgg ctcagcgttt
     2041 cggagtaggc tggagtggtg aacgcgctgc ccggcagata ggccgtcgcg taggtcacgt
     2101 cgccctggtc ggtgtagtcg tactgggcgc ggatcgagag gtgcgagtcg agcttgtgct
     2161 cgatgcctgc gccgacgatg aagccattgt gggtcttccc cagttcgccc agcttcttgt
     2221 agttgccgtt gctggtgatg ctggcgctgt ggttcgcatc gacccaggcc catccgcccg
     2281 tggcatagat catcgtatgg ctgcctaccg ccgcgccgag acgggccttg accgagatca
     2341 ggcttttcgg atcgatcttg ttggagaaca cgaagttcag cgcaggaatg ccgacgggcg
     2401 aaatcgggcc ggtaacccgc tcgttcgaac cgccgatcgt gctcgcgccc gcttcgaggc
     2461 cgaggatgat gccgccggtc tggtagttat agcccaggat cgcgccgatg tttccgttgt
     2521 tgttggactg cctggcggcc aggccatcga tgacggcagt gcgggtggcg gccgcttccg
     2581 tggaccaggt gccgcccagg gtggcggccg tatcggacct ggtggcgttc cagccggcga
     2641 tcacgccgac ataggggccg gtccaattgg cttcgtcagc cagcgcgggg gtggcgattg
     2701 ccattccgcc cagtactgaa aaaat
//
LOCUS       DATNBV010000025         2258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1614075,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000025 DATNBV010000000
VERSION     DATNBV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614075"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VN627_00650"
     CDS             complement(<1..302)
                     /locus_tag="VN627_00650"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HXD01792.1"
                     /translation="MRTFLLATTAALAVAVPTTVQAQDAAASASSPANDDAIVVTARR
                     KEESIQSVPVAIQAFSAETLVKENIHDTKDLQRLVPGVVFNGSGSELNTTFTIRGQG"
     gene            636..1130
                     /locus_tag="VN627_00655"
     CDS             636..1130
                     /locus_tag="VN627_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HXD01793.1"
                     /translation="MNSSPAPLPASPVILVDGDACPVKEEIYKVAFRREVPVVVVSNS
                     AFRIPVHPLVRRVVVSDGFDAADDWIAGEAGPRSVVVTNDILLAERCLKAGSAVLSPA
                     GRVFTAASIGGQVATRAIMADLRSGMGEALGSSGGGPAPFSKADRSRFLSALDEALVR
                     LARG"
     gene            1198..1338
                     /locus_tag="VN627_00660"
     CDS             1198..1338
                     /locus_tag="VN627_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01794.1"
                     /translation="MAKGQKKSNREARKPKAEKPKKTNASNPSIKAGVGAVAGLENLR
                     NK"
     gene            complement(1379..1687)
                     /locus_tag="VN627_00665"
     CDS             complement(1379..1687)
                     /locus_tag="VN627_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01795.1"
                     /translation="MSHPRNIVTCTGPADPHAFDGIPLRPRTGELDRPCPLCHGHGQW
                     NDEIDLASQRSIRHICPKCDGRGWIETGDDPVPTPDIERDANGHPRWITRWSPSDDRE
                     "
     gene            complement(1782..2144)
                     /locus_tag="VN627_00670"
     CDS             complement(1782..2144)
                     /locus_tag="VN627_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01796.1"
                     /translation="MLAALLLLMTAPPRVPVPRPEPAAAQFPAPAPGTAGAPVVGGFA
                     PMSRFAPELHKPFRAAVAAITPARHPGAHLLAAQRQVVAGINYRLTLKLRDGSRWQAT
                     VWHKLDGEDEVTQATRLD"
BASE COUNT          331 a          662 c          871 g          394 t
ORIGIN      
        1 ccctggccgc ggatggtgaa ggtggtgttc agttcggaac ccgaaccgtt gaagaccacg
       61 cccgggacga ggcgctggag atccttggtg tcgtggatgt tctccttgac cagggtttcg
      121 gcgctgaacg cctgaatcgc gaccggaacg gactggatgc tttcttcctt gcggcgggcg
      181 gtcacgacga tggcatcgtc attggccggc gaactcgccg aagcggcagc atcttgcgcc
      241 tgcacagtgg tgggaacagc aaccgcaagg gctgccgtgg tggctagcag aaacgtcctc
      301 atttattcct cctcagagtg ttgccatccg ggcaacctgt ggcttctcag cctctgggga
      361 aaatggctaa tgggccatac gatattcgcc ttgccctggc cggccatctt tccccgcggc
      421 gctccaagca ccggtcggcg cgcggataca agcgagaata gccttgcacc ttcgtgatcc
      481 gaattcagtg tggtctgaaa gacacacact gaggtttccg ggtcgcaaca tggatcgatc
      541 ctgcgctcta ccggtggctt gctccttcgg acaaaggcgc gggttggcgc cttgacgggt
      601 tgcgttcgac ggcttgcgcg tggcagcggg gcaggatgaa cagttcaccc gcgcccctgc
      661 cggcttctcc tgtgatcctc gtcgatggcg acgcctgtcc ggtgaaggag gagatctaca
      721 aggtggcgtt tcgccgcgaa gtaccggtgg tggtggtgag caattcggcc tttcgcattc
      781 cggtccatcc gctggtgcgg cgggtggtgg tgagcgacgg gttcgacgcg gcggatgact
      841 ggatcgctgg agaggccggg ccgcgcagcg tggtggttac caacgacatc ctgctggcgg
      901 agcggtgcct gaaggcgggg tcggcggtgc tttcgccggc ggggcgggtg ttcaccgccg
      961 cttcgatcgg cgggcaggtg gccacgcggg cgatcatggc ggacctgcgc agcgggatgg
     1021 gcgaggcgct ggggagttcc ggcggggggc cggcgccgtt ttccaaggcg gaccggtcgc
     1081 ggttcctttc ggcactggac gaagcgctgg tgcgactggc gcgcggttga gtgttcgctt
     1141 tccggcggcc gagcgcctac cctgccggcg cgggcatggg cctgcaggag aacgatcatg
     1201 gccaagggcc agaagaaatc caaccgcgag gcgcgcaagc ccaaggcgga gaagccgaag
     1261 aagaccaatg cctccaaccc ttcaatcaag gcgggcgtgg gggcggtggc cgggctggag
     1321 aacttgcgga acaagtgacc ctgcggcggt gaccggaagg ctgccggggc ggccggggct
     1381 attcccggtc gtcggagggg gaccagcggg tgatccagcg ggggtggccg ttggcgtcgc
     1441 gttcgatgtc tggcgtggga acggggtcat cgccggtttc gatccagccg cggccgtcgc
     1501 acttggggca gatgtggcgg atcgagcgct ggctggcgag gtcgatctcg tcgttccact
     1561 ggccgtggcc gtggcacagc ggacaggggc gatcgagctc gccggtgcgc gggcgcaggg
     1621 ggatgccgtc aaacgcgtgg ggatcggcgg ggccggtgca ggtgacgatg ttgcgggggt
     1681 gggacatggt gggggcggcg taacaccccc atccaacttc ggctagttcg ctgcgctccc
     1741 gagcctgcgt atccttcccc ctgccagggg gaaggggttg gtcagtcgag gcgggtggcc
     1801 tgggtgactt cgtcctcgcc atcgagcttg tgccagacgg tggcctgcca gcgcgagccg
     1861 tcgcgcagct tgagggtgag gcggtagttg atgccggcga cgacctggcg ctgggcggcg
     1921 aggagatggg cgccggggtg ccgcgccggg gtgatcgcgg cgacggcggc gcggaacggc
     1981 ttgtgcagtt cgggggcgaa gcggctcatc ggcgcaaagc cgcccaccac cggcgcgcca
     2041 gccgtaccgg gggccggagc ggggaactgc gccgccgcgg gttccggccg gggcacggga
     2101 acgcgcggcg gcgccgtcat caggaggaga agggcggcca gcatcctgct tgcaggctac
     2161 tctgcggcgg gcaggtagag ctcgctgccc ttttcgcggt agacctcgct catgtgggcc
     2221 atgccttcct ccgcctcctc ggcggcgacg aaggtttc
//
LOCUS       DATNBV010000026         3700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3848369,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000026 DATNBV010000000
VERSION     DATNBV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3848369"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VN627_00675"
     CDS             complement(<1..207)
                     /locus_tag="VN627_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HXD01797.1"
                     /translation="MSDRRSARCMWMRGGTSKGGYFLQADLPADTTARDAFLLAVMGS
                     PDPRQIDGMGGADPLTSKVAVVSKS"
     gene            complement(200..874)
                     /gene="ligK"
                     /locus_tag="VN627_00680"
     CDS             complement(200..874)
                     /gene="ligK"
                     /locus_tag="VN627_00680"
                     /EC_number="4.1.1.112"
                     /EC_number="4.1.3.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HXD01798.1"
                     /translation="MSGIVVQNIERAAPDVINGLAACGVATVHEAQRRTGLLAAYMRP
                     IYLGARVAGSAVTISAPPGDNWMVHVAIEQLKDGDILLLAPTSPCEDGYFGDLLATSA
                     QARGCRGLVIDAGVRDVRDLTEMRFPVWSKAIYAQGTVKNTLGSVNVPVVCANQLVHP
                     GDVIVADDDGVCVVRRADAEAVFKAAQVREANETDKRAKLAAGVLGLDIYKMRERLEK
                     EGLKYV"
     gene            984..2141
                     /locus_tag="VN627_00685"
     CDS             984..2141
                     /locus_tag="VN627_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXD01799.1"
                     /translation="MDLWDMNLRHLAAVAKIAELGTMNAAASAVNLTQPALTQALARI
                     EAALGLPLFERRHDGMVPTDAADLLVPRLRAASDHIASSQVTMSRMRALLALADSGSY
                     KGAAIATGLSLPSLHRAVADLSLSLRRRLVDRRGKAVVLTEAGTALVRAFRLARLELQ
                     AGFAEVEALKGHETRRIVIGAMPLSRAKVLPAAVAQFLKRHTQVRISIVEGSRAELAE
                     PLRNGMIDMMVGALRVPLMEPDLAQEELFRDQPVVVARKGHPLDGGPGKGPLSELAVL
                     AAYPWVVAGPGAPLRLAWEHMFTSAGLEPPDVPIESGSVMIIRQLLINSDLLTLLSPD
                     QVAVELEAGWLVSLGSAPAGQERTIGVTTRATWRPTVVQEEFLQELRRAAG"
     gene            2410..3231
                     /locus_tag="VN627_00690"
     CDS             2410..3231
                     /locus_tag="VN627_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HXD01800.1"
                     /translation="MERKLALIGFGEAGNTFAAAARWGLAAAAWDLLAERREAAAGLG
                     LAPAETAAQALHGASLVLSLVTADQAVPAARACAPLLERGAWWCDMNSVSPGAKREAA
                     EIVARAGARYVDVAILAPVHPGRMAVPLLLAGEQADEAEALLSEAGFTNARVVGPEIG
                     RASAIKMIRSVMVKGIEALTAEMIQAAEAAGVTDEVLASLDASDKAMPWRARAAYNVE
                     RMVTHGLRRAAEMAESATTLAELGVEPVMTAGTARRQQAMAGRKDLLIAEQGENV"
     gene            3228..>3700
                     /locus_tag="VN627_00695"
     CDS             3228..>3700
                     /locus_tag="VN627_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD01801.1"
                     /translation="MSLVIDCHGHYTVLPKAHDAWREEQKAAFKAGHPAPPYPVISDD
                     EIRETIEANQLRLIKERGADLTIFSPRASAMAPHVGDQSVAVTWAQVCNDLIGRVVGL
                     FPETFAGVCMLPQSPEADMASSVAELRRCVTDLGFVGCNLNPDPGGGRFTHPPLT"
BASE COUNT          582 a         1162 c         1338 g          618 t
ORIGIN      
        1 gctcttgctg acgacggcga ccttgctggt caacgggtcg gcgccgccca ttccgtcgat
       61 ctggcgcgga tcagggctgc ccatgacggc caggaggaag gcgtctcgag cggtggtgtc
      121 agccgggagg tccgcttgga ggaagtagcc gccctttgac gtaccgccgc gcatccacat
      181 gcaccgggcc gagcggcgat cagacatatt tcagcccttc cttctccagc cgttcgcgca
      241 tcttgtatat gtcgaggccg agcaccccgg cggcgagctt ggcgcgtttg tcggtttcgt
      301 tggcctcgcg gacctgggcg gccttgaaca ccgcctcggc atcggcgcgg cgcacaacgc
      361 agaccccgtc gtcgtcagct acgatcacgt cgcccggatg caccaactgg tttgcgcaga
      421 ctaccggcac attcaccgaa ccgagcgtgt tcttaaccgt gccttgcgcg tagattgcct
      481 tggaccagac cgggaaccgc atctcggtga gatcgcgcac atcgcgcacg ccggcatcga
      541 tgacgagacc gcggcagccg cgagcttggg cgctggtggc gaggagatcg ccgaagtagc
      601 cgtcttcaca gggtgaagtg ggcgcgagga gcaggatgtc gccgtccttg agctgttcga
      661 tggcgacatg caccatccag ttgtcgccgg gtggcgcgct gatcgtgaca gccgaaccgg
      721 ccacgcgcgc gcccaagtag atcggacgca tgtaggcggc gagcagtcct gtgcgacgtt
      781 gcgcctcgtg gacagtggcc accccacagg cggcaagccc attgattacg tcgggcgctg
      841 cccgttcgat gttctggacg acgataccgc tcatgccgat agctccactc atctgtctgg
      901 cagaggaaca gatgactccc cacagggttc ggcgaaaggc gaaacagtgg cgtggcgatt
      961 agtttttgct aaggatcgcg tccatggacc tgtgggacat gaacctgcgc cacctcgccg
     1021 ccgtggccaa gatcgccgag ctgggcacga tgaatgccgc ggcttcggcg gtcaatctga
     1081 cgcagccggc gttgacgcag gccctcgccc gcatcgaggc cgcgcttggc ctgccgctgt
     1141 tcgagcggcg gcacgacggc atggtcccca ccgatgctgc ggacctgctg gtgccgcggt
     1201 tgcgcgcggc gagcgatcac atcgccagct cgcaggtgac catgtcgcgg atgcgggcac
     1261 tgctcgccct cgccgacagc ggcagctata agggcgctgc gatcgcgacc gggttgtcgc
     1321 tgccttcgtt gcatcgcgcc gtggccgatc tctcgctgtc gctgcggcgg cggttggtgg
     1381 accgtcgggg caaggcggtg gtgttgaccg aggccggtac ggcgctggtg cgcgcattcc
     1441 ggctggcgcg actggaactg caggctggtt tcgccgaagt ggaggcgctg aaggggcacg
     1501 agacccggcg catcgtgatc ggcgccatgc cgctgtcgcg ggcgaaggtg cttccggctg
     1561 ccgtggcgca gtttctgaag cggcacacgc aagtgcggat ctcgatcgtc gaaggctcgc
     1621 gtgccgaact ggccgagccg ctgcgcaacg gcatgatcga catgatggtg ggtgccctac
     1681 gggtgccgct gatggagccg gacctggcgc aggaggagct gttccgcgac cagccggtgg
     1741 tagtcgcgcg caaaggacat cccctcgacg gcgggcctgg gaagggccca ttgagcgagt
     1801 tggctgtcct ggccgcctac ccatgggtcg ttgccggtcc tggggctccg ctgcgcctcg
     1861 cctgggagca catgttcacc agcgcggggc tcgagccgcc ggacgtaccg atcgagagcg
     1921 gctcggtgat gatcatccgc cagctgctga tcaacagcga ccttctcacc ttgctgtcgc
     1981 ccgatcaggt ggcggtggag ctcgaggctg gctggctggt ctctctcgga agcgcgccgg
     2041 cggggcagga acggacgatc ggagtcacca ctcgtgcgac gtggcgtccg acggtggtgc
     2101 aggaagagtt tctacaggaa ctacgcaggg ccgcgggttg attgttcgcg gcttgccagc
     2161 agaagcgcga gtcttgccca agcggccgct atgggttccg gatcgggtgc cggcgacgcc
     2221 acgcccggag ccggcggggg gtaggcgcgt tgcgtggaga tgatagcaat cgggcttgat
     2281 tccgaggccc gttggcgggg cggaccgagg cgccggatcg cccagggtcg gacgaggaat
     2341 agtctgtgct tatggtatta gcggcaatcc gatttgccga tcgcgagagc ggggcgcagg
     2401 ttttcaggca tggagcgcaa gctagccctg atcggatttg gagaagcggg caacactttc
     2461 gcggcggcgg ctcgctgggg cctggcggcg gcggcctggg atctcctggc ggagcggcgc
     2521 gaggcggcgg cagggctcgg gttggctccg gccgaaacgg ctgcgcaagc gctgcacggc
     2581 gcttcgctgg tgctctcgct ggtgactgcc gaccaggccg tgcccgccgc gcgggcctgt
     2641 gcccccttgc tggagcgcgg ggcgtggtgg tgcgacatga attcggtgtc tcccggcgcc
     2701 aagcgggaag cggccgaaat cgtggccagg gcaggggcgc gctatgtcga cgttgcgatc
     2761 ctggccccgg tccatcccgg ccggatggcg gtgccgctgc tgctcgcagg cgaacaagcc
     2821 gatgaggccg aggcgctgct gagcgaggcc gggttcacca acgcgcgggt ggtgggcccc
     2881 gaaattggcc gagccagcgc gatcaagatg atccgctcgg tcatggtcaa gggcatcgag
     2941 gcgctgaccg ccgagatgat ccaggccgcc gaggcggccg gggtgaccga cgaggttctc
     3001 gcctcgctcg acgcgagcga caaggccatg ccgtggcgcg cgcgggcagc ctacaatgtc
     3061 gagcgaatgg tcacgcacgg actgcgcagg gccgcagaaa tggcggagtc ggcgactacg
     3121 ctggccgagt tgggcgtaga accggtgatg acggcgggca ccgctcgccg ccagcaggcc
     3181 atggcagggc gcaaggacct gttgatcgct gagcaaggag agaatgtgtg agcctcgtca
     3241 tcgactgcca tgggcactac acagtgctgc ccaaggcgca cgacgcctgg cgtgaggagc
     3301 agaaggccgc cttcaaggcc ggccatcctg caccgcccta tccggtgatt tccgatgacg
     3361 agatccgcga gacgatcgaa gccaaccagc tgcgcctgat caaggagcgc ggggccgacc
     3421 tgacgatctt ttccccgcgg gccagcgcta tggcgccgca cgtgggcgac cagtccgtcg
     3481 cggtcacatg ggcgcaggtg tgcaacgacc tgattgggcg cgtggtcggc ctgtttccgg
     3541 agacctttgc cggcgtctgc atgctgccgc aatcgcccga ggccgacatg gccagctcgg
     3601 tcgcggaact acgccgctgc gtgaccgacc tgggcttcgt cggctgtaac ctgaaccccg
     3661 atccgggcgg cggccggttc acccatccgc cgctgaccga
//
LOCUS       DATNBV010000027         2964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1930576,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000027 DATNBV010000000
VERSION     DATNBV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1930576"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VN627_00700"
     CDS             complement(<1..470)
                     /locus_tag="VN627_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cistern family PEP-CTERM protein"
                     /protein_id="HXD01802.1"
                     /translation="MKFSYAITGLAAAAGLLVATPALADAVTLGAADIGTSFTLNYDG
                     YSGSTAISGLTGQTTFTLTGISGSTYTFDYSVLDTSSSPITGSRISGFAFNTDPTISG
                     ATSSGTYSYTNISANYPNQIGTVDVCFLGSNTGSCAGGGSGGVTQGSAAGTGSF"
     gene            complement(602..1171)
                     /gene="pth"
                     /locus_tag="VN627_00705"
     CDS             complement(602..1171)
                     /gene="pth"
                     /locus_tag="VN627_00705"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXD01803.1"
                     /translation="MQLWVGLGNPGGQYALHRHNVGFMAVDTIAEVHNFGSVQKKFQG
                     WLQEGRIGSEKILLLKPATFMNESGRSVGEALRFYKLTPADLTVFYDELDLAPMKVKV
                     RIGGGLAGHNGLRSIDQHLGPDFRRVRIGIGHPGHKDRVTGHVLGNYAKSEMDALADM
                     LGAIAAEAEWLAKGDDVRFMSDVALRQAG"
     gene            complement(1275..1889)
                     /locus_tag="VN627_00710"
     CDS             complement(1275..1889)
                     /locus_tag="VN627_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HXD01804.1"
                     /translation="MSETLTLPAEARDRVGKGASRSLRRTGRVPAVVYGENQAPQSVH
                     VEEKELVRQLGTGHFFNSIVEIVVGGETVRTLPKDVAFHPVTDRPLHADFLRISADHK
                     VHVEVPVVFANEEASPGLKRGGVLNIVRHELELVCAPDSIPDEIRIDVTGLEVGASIH
                     ISHVALPEGTASAITDRDFTIATIVAPSGLRSEEGDNSKSEAGA"
     gene            complement(1999..2838)
                     /locus_tag="VN627_00715"
     CDS             complement(1999..2838)
                     /locus_tag="VN627_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HXD01805.1"
                     /translation="MLGALLALAACARPLPADPALWEVTGPQGERAWLFGTIHALARP
                     VDWKTPKVAEALDGADSLALEIAAIDDDARTAEVFARLARSPALPPLSARVPGGLRPA
                     LAALLQQQGLAESRFAETETWAAALMLAQAQMRGLDTGNGVDRAVVAARPALPRFEFE
                     GAEAQLGIFDRLPEADQRDLLAAVVRDAGQPDDDARIADDWRKGDMAAIAAVTHTGLL
                     ADPELREALYTGRNRDWANKLEAMMRGGRHPFVAVGAAHLAGADGLPALLAARGWQVR
                     RVE"
BASE COUNT          494 a         1034 c          964 g          472 t
ORIGIN      
        1 tggaagctgc cggtgccggc cgcgctgccc tgggtcacgc cgccgctgcc gccgccggcg
       61 caggagccgg tgttcgagcc gaggaagcag acgtcgaccg tgccgatctg attggggtag
      121 ttggccgaga tattggtata gctgtaggtg cccgagctgg tggcgcccga gatcgtcgga
      181 tcggtgttga aggcgaagcc ggaaatgcgc gaacccgtga tgggcgacga gctggtgtcg
      241 agcaccgaat agtcgaaggt gtaggtgctg ccgctgatcc cggtcagggt gaaggtcgtc
      301 tgcccggtca ggccgctgat cgccgtgctg ccggaatagc cgtcatagtt cagcgtaaac
      361 gacgtgccga tatcagccgc accaagcgtg accgcgtcgg ccagcgccgg ggtcgcaacc
      421 aaaaggccgg cggcggcggc aaggccggtg atggcatagg agaacttcat ggtgactgcc
      481 ccttcaaccc aatagagtta acgggatgga aagcatggag catgccaact gaggaattca
      541 gccagtttcg atggttaacg ccctgagcgt tcaccgtcag ttgtaagccg ttccgacaat
      601 ttcagcccgc ctggcgcagg gccacgtcgc tcatgaaccg cacgtcgtcg cccttggcca
      661 gccactccgc ctcggcggcg attgctccca gcatgtcggc cagggcatcc atctcgctct
      721 tggcatagtt gcccaggaca tgccctgtca cccggtcctt gtgcccggga tggccgatgc
      781 cgatccgcac ccggcggaaa tcggggccca gatgctggtc gatcgaacgc aggccattgt
      841 gcccggcaag accgccgcca atgcggacct tgaccttcat cggggcaaga tccagctcgt
      901 catagaatac ggtgagatca gccggcgtca gcttgtagaa ccgcagcgct tcgccgaccg
      961 agcggccgct ctcgttcatg aaggtcgcgg gcttgagcag caggatcttt tcggagccga
     1021 tccgcccctc ctgcagccag ccctggaact tcttctggac ggacccgaag ttgtggacct
     1081 cggcgatggt atccaccgcc atgaagccga cgttgtgccg gtgcagggca tattgcccgc
     1141 caggattgcc gagaccgacc cagagctgca cgtccatccc tcccaacgga aaaccggcgc
     1201 aaggaaaagc cggccaaaga aaaaccggac cgcatcgctg cggcccggct ttgcttgcgt
     1261 caaccgcgca gggctcaggc gcccgcttcg gacttgctgt tgtcgccttc ttcgctgcgc
     1321 aggcccgagg gggcaacgat ggtggcgatg gtgaagtcgc ggtcggtgat cgccgaggcg
     1381 gtcccttccg gcagggccac gtggctgatg tggatcgagg cgccgacttc gaggccggtg
     1441 acgtcgatcc ggatctcgtc ggggatcgaa tccggcgcgc agaccagttc gagctcgtga
     1501 cggacgatgt tgagcacgcc gccgcgcttc aggcccggcg aggcttcctc gttcgcgaag
     1561 accaccggca cttcgacgtg gaccttgtgg tccgccgaga tgcgcaggaa gtcggcgtgg
     1621 agcggacgat cggtgacggg gtggaaggcc acgtccttgg gcagggtgcg cacggtctcg
     1681 ccgccaacga cgatctcgac gatcgagttg aagaagtgac cggtgccgag ctggcggacc
     1741 agttccttct cttcgacgtg gacgctctgg ggcgcctggt tttcaccata gacgactgcg
     1801 gggacgcggc cagtgcgacg cagactgcgg gaggctccct tgccaacccg atcgcgcgcc
     1861 tcggccggca gggtaagcgt ttcgctcatg ctcttgcctt tcgaatgcga gaaaagatca
     1921 attcatcccg ccacgcctcc agggatgacc atgcgcggga agcggcggcc cttatgggca
     1981 aagcgcccgg aatgcaagct attccacccg cctgacctgc cacccgcgcg ccgccagcag
     2041 cgccggcagg ccgtccgccc cggccagatg cgccgcgccc accgccacga acggatgccg
     2101 cccgccccgc atcatcgcct ccagcttgtt cgcccagtcc cggttgcgcc ccgtatagag
     2161 cgcctcgcgc agctccgggt cggccagcag gcccgtgtgg gtcaccgccg caatcgccgc
     2221 catgtcgccc ttgcgccagt catcggcgat ccgggcgtcg tcatcgggct ggcccgcatc
     2281 gcgcaccacg gcggccagca gatcgcgctg gtcggcctcg ggcaggcggt cgaagatccc
     2341 cagctgcgcc tcggccccct cgaactcgaa gcgcggcagg gccggccgcg ccgccaccac
     2401 tgcccggtcc accccgttgc ctgtatccag cccgcgcatc tgcgcctgcg ccagcatcag
     2461 cgcggcagcc caggtctcgg tctcggcaaa ccggctctcc gccagcccct gctgctgcaa
     2521 caaggccgcc agggcaggcc gcagcccccc cggcactcgc gccgatagcg ggggcagggc
     2581 agggcttcgc gccagccggg cgaagacctc ggcggtccgc gcatcgtcat cgatcgccgc
     2641 gatctccagc gccagcgaat ccgcgccgtc cagcgcctcg gcaaccttgg gcgtcttcca
     2701 gtccaccggc cgcgccagcg catggatcgt cccgaacagc caggcccgct caccttgcgg
     2761 cccggtcact tcccacagcg ccggatcggc cggcagcggc cgcgcacagg ccgccagcgc
     2821 cagcaaggcg cccagcacaa ctccgttcgt cctgaggagg gggtgaagcc tttggctgaa
     2881 ccgccgtctc gacggacgcg caaccatcac tgcacccgcg tcaccgtcac tccggccttg
     2941 ccaagcatgg cctgcacgct gtcc
//
LOCUS       DATNBV010000028         9660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_659350,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000028 DATNBV010000000
VERSION     DATNBV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9660
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659350"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VN627_00720"
     CDS             complement(<1..444)
                     /locus_tag="VN627_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HXD01806.1"
                     /translation="MDRRAALGLIGGGLAAVSLPARATSIALPPLELTASRLTQGGWA
                     RGIVRSGMALDLDGAAVATDGSGAFLIAFDRDAPAEARLHARAGNGWETALPLAIAPR
                     AWQIERIDAPLRPPTVPSEEFMARRKAELAQIAAARALVTPAQGWA"
     gene            complement(458..661)
                     /locus_tag="VN627_00725"
     CDS             complement(458..661)
                     /locus_tag="VN627_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HXD01807.1"
                     /translation="MLMNSSDRPATLHFGPNGFRVLRPGPFVLCAVSGQQIPLEELRY
                     WSAVRQEAYATCELATKRLLGQA"
     gene            complement(689..2152)
                     /gene="xseA"
                     /locus_tag="VN627_00730"
     CDS             complement(689..2152)
                     /gene="xseA"
                     /locus_tag="VN627_00730"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXD01808.1"
                     /translation="MPDTPPFSDDPSGGLVAKQARGDNAEPLTISEISAALKRTVEDR
                     FGFVRVRGELSGVKRAASGHLYLCLKDENARLDGVMWKGNAGRLAFRAEDGVEVVATG
                     KLTTYPGRSNYQIVIDRMEIAGEGALLALLAKTKARLEAEGLFDQRRKRALPFLPRVI
                     GVVTSPTGAVIRDILHRLADRFPSRVLVWPVLVQGNGAAEQVAAAVRGFSALQPGGAV
                     PRPDLVIVARGGGSIEDLWAFNEEVVVRAIADCAIPVISAVGHETDTTLADFAADLRA
                     PTPTAAAEIAVPVREELAAQIAELAFRKRRCALRPVQLGRERLEARVQRLPRPETIIA
                     QRVQKLDELGERLRRGLADRIAHARTALHRPAGRLSAPLLAYQLRHGRQQLDAVRLRG
                     EDLLRRVENARRQVEALDRLRRQLDPRAPLLRGYALVTGPDGHVVGGRALAAGLAEMT
                     LEFHDGSLQVSPAGAPRKAARAPRAPDGGPARQGDLF"
     gene            2151..3440
                     /gene="purD"
                     /locus_tag="VN627_00735"
     CDS             2151..3440
                     /gene="purD"
                     /locus_tag="VN627_00735"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HXD01809.1"
                     /translation="MNILLLGSGGREHALAWKLAQSALLENGGKLFAAPGNPGIADHA
                     ECVTLDTADHAAVIAFCESHHVGLVVVGPEAPLVDGLGDSLRAAGFPVFGPNRLPAQL
                     EGSKGFTKDLCARANIPTGGYERVASEEAGLAAVRRFGAPVVIKADGLAAGKGVTVAM
                     DLPTAEDAIREIFAGRFGSAGAEAVVEEFLDGEEASFFALTDGSTIVPFASAQDHKRV
                     GEGDTGPNTGGMGAYSPARVLTPALQAEAMTRIIAPTVKQLADEGMPYSGVLFLGLML
                     TAKGPQLIEYNCRFGDPECQVMLMRLESDLGELLLACAENRLSAIEPPRFSDDVALTV
                     VMAGAGYPGEPRKGGRIDGVPAAEATGAKVFHAGTNLGIDGVLTANGGRVLNVTARGK
                     SVSEAQAAAYQAVDAIHAPELFCRRDIGWREVARERG"
     gene            complement(3482..4474)
                     /locus_tag="VN627_00740"
     CDS             complement(3482..4474)
                     /locus_tag="VN627_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HXD01810.1"
                     /translation="MAAPQYDVIAIGNAIVDVMAPCSDADIARLGLAKGGMTLVDTAR
                     AHALYEAMGPAREISGGSAANTLAGLAALGAKCAFIGQVADDQLGEVFAHDIRAGGIA
                     YATPARAGDPPTARCLIFVTPDGQRTMNTYLGASQFLPSAQVDEAAIADAAVLYLEGY
                     LWDPEEPRAAMRRAIAAARNAGRKVAFTLSDSFVIARHGDDFRALIEAGEIDILFANE
                     HELAALTGIEEFHEGITHLGGKVPTVVITRSEKGAYAIAGGEHAEVAAEPVAQVVDTT
                     GAGDLFAAGFLFGLARGRALGECLKLGAICAAEIISHYGARPEADLRALVGEQG"
     gene            4585..5022
                     /locus_tag="VN627_00745"
     CDS             4585..5022
                     /locus_tag="VN627_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01811.1"
                     /translation="MRAAFLALILLTPVLAACGGSDTAPVRSTPAPVVRPHPAPRTQP
                     RAPVRRPPAPPPVQALPGVQGVIGATGADLARQFGAPRLDVIEGDARKLQFAGSSCVL
                     DIYLYPAAPGREPQASYVDARRPGDGQDVDRAACIAAMRRGRP"
     gene            5081..6040
                     /locus_tag="VN627_00750"
     CDS             5081..6040
                     /locus_tag="VN627_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01812.1"
                     /translation="MTYQFRALAEQAQADGTITAEEILALRRDGWADGRIEPAEAEAV
                     FVLNDHLAEPTPAWAEFFVEAIVQYLVSNGSPHGFITEDQADWLIARIDRDGRVDSLV
                     ELELLATLFDKAEAVPERLKAYAAAQIEQAVLTGDGPTRDGGALAPGRITPAECRLLK
                     RYIYSFTSDAPGGVSRAEAEMLFRLKDATLGADNAPEWGDLFVKAVGNHLMAHNRFQQ
                     VSADRARELDSFMNDATPRLGAFFGRMARSDLCDNIRQAAGSVLGFGKKGVARDLAGE
                     VAADQRITESEDAWLQGQVDANHQLDALDKALLRFIAEEERKG"
     gene            complement(6128..6493)
                     /locus_tag="VN627_00755"
     CDS             complement(6128..6493)
                     /locus_tag="VN627_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HXD01813.1"
                     /translation="MTETIAQTRRPRPAAVILTPGAEARVAKLMREAPEGAIGVKLST
                     PRRGCSGLAYSVDYVTEASPLDERIETPGGLFFIDSASVLYLIGSTMDWVEDDFSAGF
                     VFQNPNAKGSCGCGESFTV"
     gene            complement(6519..6998)
                     /locus_tag="VN627_00760"
     CDS             complement(6519..6998)
                     /locus_tag="VN627_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HXD01814.1"
                     /translation="MAGEPKFSVEEVEAVSSPPRARVEEALEESAADKLGRKRDYLEG
                     FLAQQPEGLAGHEPGGALYEGVIGALRDIFDPEIPVNIYDLGLIYGVDITPEGAVAVT
                     MTLTTPHCPVAESMPGEVELRVSSVPGVRDAEVNLVWDPPWDPAKMSDEAKLELGML"
     gene            complement(7013..8236)
                     /locus_tag="VN627_00765"
     CDS             complement(7013..8236)
                     /locus_tag="VN627_00765"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HXD01815.1"
                     /translation="MSAVATIDFAALRADFPGLVTRDGAPWHYLDSGASAQKPQSVID
                     ACSRAMGPDYATVHRGVYARSAEMTLAFEAARRRIAQFMGGQESEIVFTRGATEAINL
                     VAQTWGRANLKAGDRVLLSLLEHHSNIVPWQLLRDERGIEIDVVPLTPDGLIDLAAAE
                     AMITPAHKLVAFAHVSNVLGSLLDVPRAVALARSVGAKILIDGCQAVPRVPVDVAAFD
                     CDFYVWSSHKLYGPTGVGVLWGRGDLLAGMPPWQGGGAMIDRVTFAKTTFAPAPTRFE
                     AGTPAIVEAIGLAAAIDYVEAIGIGAIHAHEARLVKSLREELRAMNSVTLFGPEDSAG
                     IVSFALEGVHPHDLGTILDEENVAIRAGHHCAQPLMDHLGVPATARASFGLYNDEADI
                     AALLKGIERTRRIFG"
     gene            complement(8233..8967)
                     /locus_tag="VN627_00770"
     CDS             complement(8233..8967)
                     /locus_tag="VN627_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HXD01816.1"
                     /translation="MSAALPLPTRKDEDFRYADFGALERVWPVAVEDIAVAAGESRAL
                     VLVPEGENVARHLAIALAPGATFDLRILNAGHAYGRVAVDVTLGEGASFTLGAAQVGG
                     GKQVLELVTNVVHAGLNAQSRQVVRSVLGGEATGTYLGKVMVARGADGTDGEQSVRAM
                     LLDRTATANARPELEIYADDVKCAHGCAVGELDAQGLFYLQSRGLPPAEAKRLMLQAF
                     LAEAFVGAEQEEALDAAALAALEALL"
     gene            complement(8964..9659)
                     /gene="sufC"
                     /locus_tag="VN627_00775"
     CDS             complement(8964..9659)
                     /gene="sufC"
                     /locus_tag="VN627_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HXD01817.1"
                     /translation="MLKGLSLTVNAGEIHAIMGPNGAGNSTTGYVLGGRPGYAVTGGS
                     VMFNGEDLLALEPHERAAAGLFLGFQYPVEIPGVSFVQFLREAANAQRRVRGEEPLSG
                     GEFLKLAREKAGLLRMDMDMLKRPVNVGFSGGEKKRAEMVQMGILDPTLAILDETDSG
                     LDIDALRICGEGINAIMRKPDKAVILITHYQRLLDYVKPDFVHVLAAGRIVRSGGPEL
                     AHELEREGYEAVA"
BASE COUNT         1525 a         3510 c         3230 g         1395 t
ORIGIN      
        1 ggcccagccc tgagccggcg taaccagggc ccgggcggcg gcgatctgcg ccagttcggc
       61 cttgcggcgg gccatgaatt cctcgcttgg caccgtcggc gggcgcagcg gcgcatcgat
      121 ccgctcgatc tgccaggcgc gcggagcgat ggcgaggggc agggccgttt cccagccatt
      181 gcccgcccgc gcatggaggc gggcctctgc cggcgcatcg cgatcaaagg cgatcaggaa
      241 agcgcccgag ccgtcagtcg ccacggccgc gccatcgagg tcgagcgcca tgcccgagcg
      301 gacgatcccg cgcgcccagc cgccctgggt cagccggctg gcggtgagct caagcggagg
      361 caaggcgatc gaggtcgcgc gggcaggcag gctcactgcc gccagaccgc cgccgatcag
      421 ccccagcgca gcccggcgat ccatgtcggg gaggcgctca ggcttggcca agcagccgct
      481 tcgtggccag ctcgcaagtg gcataggcct cctggcgcac cgcgctccag tagcgcagct
      541 cctccagtgg gatctgctgg ccgctgacgg cgcagagcac gaaggggccg gggcgcagga
      601 cgcggaagcc gttggggccg aagtgaagag tggcgggacg gtcggacgag ttcatcagca
      661 tgggggcaag gctaacagtc ttcgccgctc agaacaaatc gccctgccgg gcaggaccgc
      721 catccggcgc gcggggcgcc cgcgccgcct tgcgcggagc cccggcgggg ctgacctgga
      781 gcgagccgtc atggaattcc agcgtcatct cggcaagccc cgcagcgagg gccctgccgc
      841 cgacgacatg gccatcgggc ccggtcacca gcgcatagcc gcgcagcagc ggagcgcgcg
      901 gatcgagctg gcggcgcagg cgatcaagcg cctcgacctg gcggcgggcg ttctccaccc
      961 gccgcaacag gtcctccccc cgcagccgca ccgcatcgag ctgctgacgc ccatggcgca
     1021 actggtaggc cagcagcggg gcggagagcc gtccggccgg gcggtgcagc gcggtgcggg
     1081 catgggcgat ccggtcggcc aggccccgcc gcagccgctc gcccagctcg tcgagcttct
     1141 gcacgcgctg ggcgatgatg gtctccggcc ggggcaggcg ctggacccgc gcctcgagcc
     1201 gctcgcgccc gagctggacc ggacggagcg cacagcgtcg cttgcggaag gccagctcgg
     1261 cgatctgcgc ggccagctcc tcgcgcacgg gcacggcgat ctcggcggcg gccgtgggcg
     1321 tgggcgcgcg caggtcggcc gcgaagtccg caagcgtggt gtcggtctca tgccctaccg
     1381 cgctgatcac cgggatcgcg caatcggcaa tggcgcgcac caccacttcc tcgttgaagg
     1441 cccacaggtc ctcgatcgag ccaccgccgc gcgccacgat cactaggtcc ggtcgcggta
     1501 cggcaccgcc gggctggagc gcggaaaagc cccgcactgc cgccgccacc tgctcggccg
     1561 cgccattgcc ctggaccagc accggccaca ccagcacgcg gctggggaac cgatcggcca
     1621 gccggtgcag gatatcgcga atgaccgcgc cggtgggcga tgtcaccacg ccgatcaccc
     1681 gcggcaggaa gggcagcgcc cgcttgcggc gctgatcgaa caggccttcg gcctccagcc
     1741 gcgccttggt cttggccagc agcgccagca gcgcgccttc gccggcgatc tccattcgat
     1801 cgatcacgat ctggtagttt gagcggcccg gataggtcgt cagcttcccc gtcgcgacga
     1861 cttccacgcc gtcctcggcg cggaacgcga gccgtccggc attgcccttc cacatcaccc
     1921 cgtcgagccg ggcattctcg tccttgaggc acagataaag atgcccgctc gccgcccgct
     1981 tcacgccgga aagctcgccg cgcacccgca cgaagccgaa cctgtcctcc accgtgcgct
     2041 tcagggccgc tgaaatctcg ctgatggtca acggctcggc attgtcgccg cgcgcctgct
     2101 tcgctaccag cccgccggac ggatcatcgg aaaaaggcgg cgtatcgggc atgaacatcc
     2161 tgctcctggg ctcgggcggg cgcgaacatg cgctggcgtg gaagctggcg caatcggctt
     2221 tgctggaaaa tggcggcaag ctcttcgctg cccccggcaa tcccggcatc gccgatcacg
     2281 ctgaatgtgt caccctcgat acggccgacc acgccgccgt catcgccttc tgcgagagcc
     2341 atcacgtcgg cctcgtcgtg gtcggcccgg aagcgccgct ggtcgatggg ctgggcgata
     2401 gcctgcgcgc cgccgggttc ccggtgttcg ggcccaatcg cctgccggcc cagctcgaag
     2461 gctcgaaagg cttcaccaag gacctctgcg cccgcgccaa tatccccacc ggcggctacg
     2521 agcgcgtcgc cagcgaggaa gcaggtctgg ccgccgttcg ccgcttcggc gcgccggtgg
     2581 taatcaaggc cgatggcctc gccgcgggca agggggtgac cgtcgccatg gaccttccca
     2641 cggccgagga cgcgatccgc gagatctttg ccgggcgctt cggctcggcg ggggcggagg
     2701 ccgtggtcga ggaattcctc gacggcgagg aagcgagctt cttcgccctg accgacggct
     2761 ccaccatcgt gcccttcgct tccgcgcagg accacaagcg ggtgggcgag ggcgacacgg
     2821 ggccgaacac cggcggcatg ggcgcctaca gcccggcccg cgtgctcact cccgcgctgc
     2881 aggccgaggc gatgacccgg atcatcgccc cgacggtcaa gcagctggcc gatgagggca
     2941 tgccctattc cggcgtcctg ttcctcggcc tgatgctcac cgccaagggc ccgcagctga
     3001 tcgaatacaa ttgccgcttc ggcgatccgg aatgccaggt catgctgatg cggctggaga
     3061 gcgatctggg cgaactgctg ctggcctgtg cggaaaaccg cctctccgcc atcgaaccgc
     3121 cgcgcttctc cgacgatgtc gccctgaccg tggtcatggc cggcgccggc taccccggcg
     3181 agcccaggaa gggtggccgg atcgacggcg tgcccgccgc cgaagccacc ggcgccaagg
     3241 tgttccacgc cggcaccaac ctcggcatcg atggcgtgct gaccgccaac ggcggccgcg
     3301 ttctcaacgt caccgcgcgg ggcaagtcgg tgagcgaagc gcaggcggca gcctaccagg
     3361 ccgtcgacgc catccacgcc ccggagctgt tctgccgccg ggacatcggc tggcgcgaag
     3421 ttgcccgcga gcgagggtga ccctcagatc ctccccgcac cgcggggagg atcgcgaaaa
     3481 atcacccctg ctcgcccacc agcgccctga ggtccgcctc gggccgcgcg ccgtagtgcg
     3541 agatgatctc cgccgcgcag atcgcgccca gcttcaggca ttcccccagc gccctgccgc
     3601 gcgccaggcc gaacaggaag ccggcggcaa acaggtcgcc agccccggtc gtgtctacca
     3661 cctgcgccac tggctcggcc gcaacctcgg catgctcgcc gccggcaatg gcgtaggcgc
     3721 ctttctcgct gcgggtgatc actacggtcg gcaccttgcc gcccaggtgc gtgatcccct
     3781 catggaactc ctcgatcccg gtcagcgcgg ccagttcgtg ttcgttggcg aagagaatgt
     3841 cgatctcccc ggcctcgatc agcgcgcgga aatcgtcgcc gtggcgggcg ataacgaagc
     3901 tgtccgacag ggtgaaggcc accttgcgcc ccgcattccg cgccgccgca atcgcccggc
     3961 gcatggccgc gcgcggctct tccgggtccc acaggtagcc ttcgagatag agcacggcgg
     4021 catcggcaat ggccgcctcg tccacctgcg ccgaaggcag gaactgcgag gcaccgaggt
     4081 aggtgttcat cgtgcgctgc ccatcgggcg tgacgaagat caggcaccgc gccgtgggcg
     4141 gatcgccggc acgggccgga gtggcatagg cgatcccgcc ggcgcggata tcgtgggcga
     4201 agacctcgcc cagctggtca tcggcgacct gcccgatgaa ggcacacttc gcaccgagcg
     4261 cggcaaggcc cgccagcgta ttggccgccg aacctccgga aatctcgcgc gccgggccca
     4321 tggcctcgta aagcgcgtgc gcgcgggccg tatcgaccag tgtcatgccg cccttggcga
     4381 ggccgaggcg cgcgatatcg gcatcgctgc agggggccat cacgtcgaca atggcattgc
     4441 cgatggcgat cacgtcatat tggggagcgg ccatgccggt acctttggcg ggaggaagcg
     4501 ttgcggggcg actagcggcg ggccggggca ccggcaagcc cgatcgccgt tgacctcgcc
     4561 gccccggcag gcgatggccg cgccatgcgc gccgcgttcc tcgctctcat cctgctaact
     4621 ccggtcctcg ccgcctgcgg ggggagcgac accgccccgg tgcggtcgac gccggcgccg
     4681 gtcgtgagac cgcacccggc cccgcgcacc cagccccgcg cccccgtccg ccgcccgccg
     4741 gccccgccgc cggtccaggc cctgcccggc gtacagggcg tgatcggcgc gaccggggcg
     4801 gaccttgccc gccagttcgg cgcgccccgg ctcgacgtga tcgaaggcga tgcccgcaag
     4861 ctgcagttcg ccggcagttc ctgcgtgctg gacatctacc tctatccggc cgcccccggg
     4921 cgcgagccgc aggcaagcta tgtcgatgcg cggcgccccg gcgacggaca ggatgtggac
     4981 cgcgccgcct gcattgccgc catgcgtcgc ggccggccgt aacccgccct taaccgcaat
     5041 cgatgcatag ccgccccatg caacgggggg caccacgcct atgacttacc agttccgcgc
     5101 gctggccgag caggcccagg ccgatggcac gatcactgcc gaagagattc tcgccctgcg
     5161 ccgcgacggc tgggccgacg gccggatcga accggccgag gccgaggccg tgttcgtcct
     5221 caacgatcac ctggccgaac cgaccccggc ttgggccgag ttcttcgtcg aggcgatcgt
     5281 gcagtacctc gtgtcgaacg gcagcccgca cggcttcatc accgaagacc aggccgactg
     5341 gctgatcgcc cggatcgacc gcgatggccg ggtcgattcg ctggtcgaac tcgaactgct
     5401 cgccactctg ttcgacaagg ccgaggccgt gcccgaacgg ctcaaggcct atgccgcggc
     5461 ccagatcgag caggccgtgc tcaccggcga tggccccacc cgtgacggcg gcgccctggc
     5521 ccccgggcgg atcactccgg ccgaatgccg cctgctgaag cgctacatct attccttcac
     5581 gagcgacgcc cccggcggcg tcagccgggc cgaagccgaa atgctgttcc gcctcaagga
     5641 cgcaacgctc ggcgccgaca acgcgcctga atggggcgac ctgttcgtca aggccgtcgg
     5701 caatcacctg atggcccaca accgcttcca gcaggtttcc gcggatcgcg cccgcgagct
     5761 cgacagcttc atgaacgatg cgaccccgcg gctcggcgcc ttcttcggcc gcatggcccg
     5821 gagcgacctc tgcgacaaca tccgccaggc cgccggcagc gtgctcggct tcggcaagaa
     5881 gggtgtcgcg cgcgacctcg ccggcgaagt agccgccgac cagcggatca ccgaaagcga
     5941 ggatgcctgg ctgcagggcc aggtcgatgc caaccaccaa ctcgatgcgc tcgacaaggc
     6001 gctgctgcgc ttcatcgccg aggaagaacg caaggggtga gtcttcaggt gtgtacggaa
     6061 aggcctttcc ggcctttcct acgcggccgg tgccgcaagc ccttggcgga tgccaagggc
     6121 gcacacttca aacagtaaag ctctctccgc agccgcagct gcccttggca ttggggttct
     6181 ggaacacgaa cccggcggag aaatcgtcct ccacccaatc catcgtgctg ccgatcaggt
     6241 aaagcaccga ggcgctgtcg atgaagaaca ggccgccggg cgtctcgatc cgctcgtcca
     6301 gcgggctggc ctcggtcacg tagtccaccg aataggccag gcccgaacag ccccggcgcg
     6361 gggtggaaag cttgacgccg atcgcgccct cgggcgcctc gcgcatcagc ttggccacgc
     6421 gcgcctcggc gcccggcgtg aggatcacgg cggcggggcg ggggcggcgt gtctgggcaa
     6481 tcgtttcggt catggctgca acctatctgg ggggcggatc acaacatgcc aagttcaagt
     6541 ttggcttcat cgctcatctt ggccggatcc cacggcggat cccacaccag gttcacttcc
     6601 gcatcgcgca cgccgggcac cgaactgacc cgcagctcca cctcgccggg catcgattcg
     6661 gcaaccgggc agtggggcgt ggtcagcgtc atggtgacgg ccaccgcgcc ttccggcgtg
     6721 atgtccacgc cgtagatcag cccgaggtca tagatgttca ccggaatttc cggatcgaag
     6781 atatcgcgca gggccccgat cacgccctca tagagcgcac cgcccggctc gtgccccgcc
     6841 agcccctcgg gctgctgcgc gaggaagccc tcaaggtagt cgcgcttgcg accgagcttg
     6901 tcggcggcgc tctcctcgag cgcctcctcg acacgcgcgc ggggcggcga ggacacggcc
     6961 tcgacctctt ccacactgaa cttgggttca ccggccatgc tggattcggc ggtcatccga
     7021 agattctcct ggtgcgttcg atgcccttca gcagggcggc aatatcggct tcgtcattat
     7081 agagcccgaa cgacgcgcgg gccgtcgccg gcacgccgag atggtccatg agcggctgcg
     7141 cgcagtgatg cccggcacgg atggcgacgt tctcctcgtc gagaatcgtg ccaagatcgt
     7201 gcgggtgcac gccttcaagc gcgaagctga cgatcccggc gctgtcctcc ggaccgaaca
     7261 gggtcacgct gttcatcgcc cgcagctcct cgcgcaggga cttcaccagc cgcgcctcgt
     7321 gagcatggat tgcgccgatc ccgatcgcct cgacatagtc gatcgcagcg gccagcccga
     7381 tcgcctcgac gatcgccggc gtgcccgcct cgaaccgggt gggcgcgggg gcgaaagtgg
     7441 tcttggcgaa agtaacgcgg tcgatcatcg ccccgccgcc ctgccatggc ggcatgcccg
     7501 ccagcaggtc gccccggccc cacagcacgc caaccccggt cgggccgtag agcttgtggc
     7561 tcgaccagac gtagaaatcg cagtcgaacg cggcgacatc caccggcacg cgcggcacgg
     7621 cctggcaccc gtcgatcagg atcttcgcac cgaccgagcg cgccagcgcg accgcccgcg
     7681 gcacgtcgag caatgagccc agcacgttcg acacatgggc aaaggccacc agcttgtgcg
     7741 ccggggtgat catcgcctcg gccgcggcca agtcgatcag gccatccggg gtaagcggga
     7801 cgacatcgat ctcgatcccg cgctcgtcac gcagcagctg ccaggggacg atgttggaat
     7861 ggtgttcgag cagggagagc agcacccggt cgccagcctt gagattggcc cggccccagg
     7921 tctgcgccac aagattgatc gcctcggtcg cgccgcgggt gaagacgatt tcggactcct
     7981 gcccgcccat gaactgcgca atccggcgcc gggcggcctc gaaggccagc gtcatctcgg
     8041 ccgagcgggc gtagacgccg cggtggacgg tcgcatagtc cggccccatg gcgcggctgc
     8101 aggcgtcaat cacgctttgc ggcttctgcg ccgaagcgcc gctgtcgagg tagtgccacg
     8161 gcgcgccgtc gcgggtgacc aggccgggga aatcggcgcg cagggcggca aaatcgatgg
     8221 tggccacggc gctcacagca gggcctccag ggcggcaaga gcagcggcgt cgagcgcctc
     8281 ttcctgctcc gcgccaacaa acgcctcggc gaggaaagcc tgcagcatca gccgcttggc
     8341 ctccgccggg ggaagcccgc gcgattgcag atagaacagc ccctgcgcgt cgagctcacc
     8401 caccgcgcag ccgtgggcgc acttcacgtc gtcggcatag atttccagtt cgggccgggc
     8461 attggccgtg gccgtccggt cgagcagcat ggcgcggacc gactgttcgc cgtcggtgcc
     8521 atcggccccg cgcgcgacca tgaccttgcc gagataggtc cccgtcgcct cgccgccgag
     8581 gaccgagcgc acgacctgcc ggctctgcgc gttcaggccg gcgtggacca cgttggtcac
     8641 cagctccagc acctgctttc cgccgccgac ctgcgccgcg ccgagtgtga acgatgcccc
     8701 ttcgccaagg gtcacgtcca ccgccacccg gccataggca tggcccgcgt taagaatgcg
     8761 caggtcgaac gtcgcacccg gagccagcgc gatcgcgaga tgccgggcaa cgttctcacc
     8821 ctcgggcacc aataccagcg cgcgcgattc gcccgctgca accgcgatgt cctccaccgc
     8881 caccggccag acgcgctcca gcgcgccgaa atcggcatag cggaagtcct catccttgcg
     8941 ggtgggcaga ggcagggctg cgctcacgcc acggcctcgt aaccctcgcg ctcgagttcg
     9001 tgcgccagtt ccggcccgcc gctgcgcacg atccgtccgg cagccagcac gtgcacgaag
     9061 tccggcttca cgtagtcgag caggcgctgg tagtgggtga tcaggatcac cgccttgtcg
     9121 ggcttgcgca tgatcgcgtt gatgccctcg ccgcagatgc gcagggcgtc gatgtcgagg
     9181 cccgaatcgg tctcgtcgag aatcgccagg gtggggtcaa ggatgcccat ctggaccatc
     9241 tccgcccgct tcttctcgcc gccggaaaag cccacgttca cggggcgctt gagcatgtcc
     9301 atgtccatgc gcagcaggcc cgccttctca cgcgccagct tgaggaactc gccgccggaa
     9361 agcggctctt ccccgcgcac cctgcgctgg gcattggccg cctcgcgcag gaattggacg
     9421 aaggagacgc ccgggatctc gaccgggtac tggaaaccga ggaacagccc ggcggccgcg
     9481 cgctcgtgcg gttcgagcgc gagcaggtcc tcgccattga acatgaccga gccgcccgtc
     9541 accgcatagc ccggacgccc gcccagcacg tagccggtgg tcgaattgcc tgcgccgttc
     9601 ggccccatga tcgcatggat ctcgcccgcg ttcacggtga ggctgaggcc cttgaggatg
//
LOCUS       DATNBV010000029         2473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3849133,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000029 DATNBV010000000
VERSION     DATNBV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3849133"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1638
                     /locus_tag="VN627_00780"
     CDS             235..1638
                     /locus_tag="VN627_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXD01818.1"
                     /translation="MNLFVHLILRLAGVVLVCLVCAVGWVLIDSHRAIEKEAVASADR
                     VAGTLEALYWRELLWRGGLNREHLVPIPDWDTMATMKVISPGICITVAPGKEAPRRLC
                     SQLETISEPAPAWFKAFYDHVFGGYARVERPLTLRQPMAGLVAAEPDSGAAVRQAWHQ
                     TAVVLSVAALLAAGIGILAALFIGQSLMPARTIVAGLRRLGRGDYSYRLPSYRTAEFN
                     QISRAVNDLTTRLAETTAQRVALTNRLFQVQEEERRALARDLHDEFGQCLAAVGALAT
                     VIEMEAASGQPEIAKDARSIIDVTKRMTATLRGALARLRSQEVEELGLEASLAQLVAG
                     WNLQTEPEAIFHLDIVGDLAKLPEATALNIYRIAQECLTNAARHGKPQDVRLHVARVD
                     AEGSGAVALSVEDDGGGSPAKLSSASGYGILGIRERVAALGGSLSIGQARRGVRVAAL
                     IPLMPPRESSGATPVPA"
     gene            1635..2294
                     /locus_tag="VN627_00785"
     CDS             1635..2294
                     /locus_tag="VN627_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXD01819.1"
                     /translation="MTELTILLVDDHPVVREGYRRLLERTPGYRVVAEAHDAASAYQA
                     YQKAKPDVVVMDLSLPGVGGIEALRHIRQWDHGARILIFTMHSGAGFALKAFEAGAAG
                     YVTKGSDAAELVSSVAIVAKGGRALSNDVAREIAVERLAHPRSPLDELSPRETEILRL
                     IASSWSAEEISASLHLSLKTVRNYHYQIKAKVGARTDAHLVWLAIGAGLVQAGDQPPS
                     S"
BASE COUNT          391 a          820 c          829 g          433 t
ORIGIN      
        1 gatcgcgccc gggcagttcg aagctgcgct cgttcatgcg gatatggctc cggcggctct
       61 tgggcgtctc ggagcttatc ggtcggcaag gcaaatggcc tgggcaccga tcctcattcg
      121 ccttgggcaa tatgcccgac ccggttccct ggggtggccg gtcgggcccc cgagcttgcc
      181 aagccaagcc cgcgcgacca gtatgagaaa ggccaatttg gatgagagag taaaatgaac
      241 ctatttgttc atctcatctt gcgtctcgcc ggcgttgttc ttgtctgcct ggtctgcgcc
      301 gtcggctggg tgctgatcga ttcgcatcgg gccatagaaa aggaagcggt cgcatcggcc
      361 gaccgggtcg ccggcacgct cgaggcgctc tattggcgcg aattgctgtg gcgcggcgga
      421 ctcaaccggg agcatctggt gcctatcccg gactgggaca ccatggcgac catgaaggtc
      481 atctcgccgg gcatctgcat caccgtcgcg cccggcaagg aagcgcctcg gcgcctgtgc
      541 agccagctgg agacgatcag cgagccggcg ccggcctggt tcaaggcctt ctatgaccat
      601 gtcttcggcg gttatgcccg ggtcgagcgg cccttgacgc tgcggcagcc catggccggc
      661 ctggtcgcag ccgaaccgga tagcggcgcc gcggtgcgcc aagcctggca tcaaaccgcc
      721 gtcgtgctca gcgtcgcggc gttgctcgcc gccgggatcg gcattctcgc cgccttgttc
      781 atcgggcaat cgctgatgcc cgcccgcacc atcgtcgccg ggctgcggcg gctggggcgt
      841 ggcgactaca gctaccgcct gcccagctat cgcacggcgg agttcaacca gatttcccgc
      901 gcggtgaacg atctgaccac ccgtttggcg gagacgaccg cgcagcgcgt cgcgctgacc
      961 aatcgcctgt tccaggtgca ggaagaggaa cgccgggcgc tggcgcgcga tctccatgac
     1021 gagttcggcc agtgccttgc cgcggtcggg gctttggcca cggtgatcga gatggaggca
     1081 gcgtcgggcc agccggagat cgccaaggac gcacgctcga tcatcgacgt caccaagagg
     1141 atgacggcga cgcttcgcgg cgccctggcc cgtctgcgct cgcaggaggt cgaggagctc
     1201 ggcctggaag cgagcctcgc ccagctcgtc gcggggtgga atctgcagac cgaaccggag
     1261 gcgatcttcc acctcgacat tgtcggcgat ctggccaaac tgcccgaggc gaccgccctg
     1321 aacatctacc gcatcgccca ggaatgcctg accaatgccg ctcgccatgg gaaaccgcag
     1381 gatgtccgcc tgcatgtggc gcgtgtcgat gcggagggaa gcggtgccgt ggccctcagc
     1441 gtggaggatg atggcggcgg cagcccggcc aagctgagct cagcatcggg atatggcatc
     1501 ctcggcatcc gcgagcgggt ggcggcgctc ggcggcagcc tgtcgatcgg tcaggctcgg
     1561 cgcggggtgc gcgtggccgc tctcattccc ttgatgcccc cacgcgaatc atccggcgca
     1621 acaccggtac cggcatgacc gagctcacca tccttttggt cgacgatcat cccgtcgtgc
     1681 gggagggcta tcggcggctg ctcgagcgga cgccgggata ccgggtcgtg gcggaggccc
     1741 atgatgccgc ttccgcctat caggcctatc agaaggcgaa gcccgatgtc gtggtcatgg
     1801 atctctccct ccccggcgtc ggcgggatcg aagcgctgcg gcatatccgg caatgggatc
     1861 acggcgcacg cattctcatc ttcaccatgc acagcggcgc cggctttgcc ttgaaggcct
     1921 tcgaggcggg tgccgccggc tatgtcacca agggaagcga cgccgccgag ctcgtcagtt
     1981 cggtcgcgat cgtcgccaag ggcggccggg ctctgagcaa cgatgtggcc cgggagatcg
     2041 ccgtcgagcg cctcgcccat ccgaggtccc ccttggacga gctcagcccg cgtgagaccg
     2101 agatcctccg cctcatcgcc tcgagctggt cggcggagga gatttcggcc tcgctccatc
     2161 tcagcctcaa gaccgtccgc aactaccact accagatcaa ggccaaggtc ggcgcacgca
     2221 ccgatgccca tctggtgtgg ctcgccatcg gcgccggcct cgtccaagcc ggcgatcaac
     2281 cgccgagctc ttgatgcggc agcggggata gcttgacggc gtccccggcc cgggcaaggg
     2341 cgggaacgcg gatcgtccgg ctctgtttcg gcggcggttt aacggcgcct taaccgctct
     2401 ccatcatagg ctgttgcgca accggtccgt cgatcggttg aacctttcga ggcgggattc
     2461 ggatcgtggt ctc
//
LOCUS       DATNBV010000030         3426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2890270,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000030 DATNBV010000000
VERSION     DATNBV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2890270"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="VN627_00790"
     CDS             complement(<1..1818)
                     /locus_tag="VN627_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXD01820.1"
                     /translation="MPTNRRKPRPPKPRQATRFGLDEIVVTASARPANRLDSSISVST
                     LSDSAIAEAAPRTTAEIFRQIPGIRSESTGGDGNANIAVRGLPVASGGAKFLQLQEDG
                     MPVMQFGDIAFGNADIFLRADQTVASIQAVRGGSASTQASNAPGGVINFLSKTGKEDG
                     GTIMATAGIDYREYRLDFDYGGRIAEHTLFNVGGFWRQGEGPRRAGYDGNSGYQIKAN
                     LTQEFDGGYVRLYAKRLDDKAIGYLPMPTLVTGTNANPRFQSFPGFDISKDTPHSALF
                     TTDAGLDGNNNPTTTDIRDGMHPVVTSLGLEAVFDVADGVKLEERFRWSDVHGGFVSP
                     FPSEVAGAQSIANSVGELLTGTPGTYGLAYANGPNAGQAINPATLNGNGLLMRTHLFN
                     VKINDFGSFTNDLKLSRSLGKGTLTAGFYKARQTIDMDWVWNSYLLEVKGNNAALVNV
                     VAPGGEVLSRNGLYAYGVPFWGNCCQRSYNVTYDIDAPYAALNLDLDQLSIDASIRYD
                     HVRARGNYAGTLQVANRDVNGDGTIQPVERSVSLIDNASPSPVNYSVGYVSWSVGANY
                     RVSADAAVFARASRSGRANADRLLFGVVRPDGSVRKDDAI"
     gene            2050..3363
                     /locus_tag="VN627_00795"
     CDS             2050..3363
                     /locus_tag="VN627_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD01821.1"
                     /translation="MQEADKPRLSLARIIEMNIGFLGLQFSFGLQQANMGPIYGFLGA
                     NEATMPLLWLAGPMTGLIVQPLIGAMSDRTHSRLGRRTPYFLIGAVLCSLCLLLMPLS
                     GAVWMAASLLWILDAGNNITMEPYRAYVADRLRPGQRAVGFLTQSAFTGLAQTLSYLA
                     PTLLTSVVSRQVLDANGIPAVVRIAFAIGAVLSISTIVWSIWRVPELPLEPAEAAALA
                     ARPMTLRATLAEIGSACREMPPAMRQLALAMLCQWYAMFAYWQYIAFAVGRSLYHTHD
                     ATSSAFRDATLTAQQAGAFYNLVAFIGALGLIPVVRRIGARRAHALCLTASGAAMLAI
                     SHSGSMAVLFAAMVGIGLGWAGMMGNTYVMLADAIPPERNGIYMGIFNMFIVIPMLIQ
                     SLTLPLLYDGLLGGRPENVIRLAGVLLGLAALCVLRVKTSALNQR"
BASE COUNT          522 a         1062 c         1203 g          639 t
ORIGIN      
        1 gatcgcgtcg tccttgcgga ccgagccgtc gggccggacc acgccgaaca gcagacgatc
       61 ggcgttggcg cggccgctgc ggctggcgcg ggcgaacacc gccgcgtcgg ccgagacgcg
      121 gtagttggcg ccgaccgacc acgagacata gccgacgctg tagttgaccg ggctcgggct
      181 ggcattgtcg atcagcgaga cgctgcgttc gaccggctgg atcgtgccgt caccgttgac
      241 gtcgcgattg gccacctgca gcgtgccggc ataattgccg cgggcgcgga catgatcgta
      301 gcggatgctg gcgtcgatgg acagctggtc gagatcgagg ttgagggcgg catagggcgc
      361 gtcgatatcg taggtgacgt tgtagctgcg ctggcagcaa ttgccccaga agggcacccc
      421 ataggcatag aggccattgc ggctgagcac ctcgccgccg ggggcgacga cgttgacgag
      481 ggctgcattg ttgcccttca cctcgagcag gtaggagttc cacacccagt ccatgtcgat
      541 ggtctggcgg gccttgtaga agcccgcggt gagggtgccc ttgcccaagc tcctggagag
      601 cttaaggtca ttggtgaagc tgccgaaatc gttgatcttc acgttgaaca ggtgggtgcg
      661 catgagcagc ccgttgccgt tcagcgtggc ggggttgatc gcctggccgg cgttggggcc
      721 attggcgtag gcgagaccgt aggtgccggg ggtgccggtg agcagttcgc cgaccgagtt
      781 ggcgatcgac tgggcgccgg ccacttccga ggggaagggc gagacgaagc cgccgtggac
      841 atcggaccag cggaagcgct cttccagctt tacgccgtca gccacgtcga acacggcttc
      901 gaggccgagc gaggtgacca ccggatgcat gccgtcgcgg atgtcggtgg tggtggggtt
      961 gttgttgccg tcgagcccgg catcggtggt gaacagggcg ctgtgcggcg tgtccttgct
     1021 gatgtcgaag ccggggaacg actggaacct gggattggcg ttggtgccgg tgacgagggt
     1081 cggcatgggc aggtagccga tcgccttgtc gtcgagccgc ttggcataga ggcgcacgta
     1141 gccgccgtcg aattcctggg tgaggttggc cttgatctgg tagccgctgt tgccgtcgta
     1201 gccggcccgg cgcgggcctt cgccctggcg ccagaacccg cccacgttga acagggtgtg
     1261 ctcggcgatg cggccgccgt aatcgaagtc gaggcggtat tcgcggtagt cgatcccggc
     1321 cgtcgccatg atcgtgccgc cgtcttcctt gccggtcttc gacaggaagt tgatcacgcc
     1381 gcccggcgcg ttcgaggcct gggtcgaggc cgagccgccg cgcacggcct ggatcgaggc
     1441 gaccgtctgg tcggcgcgca ggaagatgtc ggcattgccg aaagcgatgt cgccgaactg
     1501 catgacgggc atgccgtctt cctgcagctg gaggaacttg gcgccgccgg aggccacggg
     1561 caggccgcgc acagcgatgt tggcattgcc gtcaccgccg gtcgattccg aacggatacc
     1621 ggggatctgg cggaagatct cggccgtagt gcgcggcgcg gcttcggcaa ttgcgctgtc
     1681 ggacagggtc gagaccgaga tcgagctgtc gagccggttg gcggggcggg ccgaggcggt
     1741 gacgacgatc tcgtcaaggc cgaagcgcgt cgcttgccgg ggcttcggcg gccggggttt
     1801 ccgccggttt gttggcattg tcctgcgcga aggcaggggc ggacagggcc gatagcgcga
     1861 cgccgagggc caatgcgtga cgaacgttca tggtggtgtt ttcctctccc gaaaacgatt
     1921 cgtcattatg gatatgccgg attcaaacgt ttgcaaacac aaaaatgcaa acgtttgaaa
     1981 tttgccccgg ggtgtgtcac tgtccacaca gatagcgccg gcgaagcgct ctgtggagtg
     2041 gatgcgacca tgcaagaggc tgataaaccg cggctttccc tcgcgcgcat tatcgagatg
     2101 aacatcggat tccttgggct gcaattcagc ttcgggctcc agcaggccaa tatgggcccg
     2161 atctatgggt tcctcggtgc caacgaggcg accatgccat tgctgtggct ggccggcccg
     2221 atgaccgggc tgatcgtgca gccgctgatc ggggcgatga gcgaccgcac ccattcgcgg
     2281 ctggggcggc gcacgcccta tttcctgatc ggcgcggtgc tctgttccct gtgcctgctg
     2341 ctgatgccgc tctccggcgc ggtgtggatg gccgcttcgc tgctgtggat cctcgatgcc
     2401 ggcaacaaca tcacgatgga gccctaccgt gcctacgtcg ccgaccggct gcggccgggc
     2461 cagcgggcgg tgggcttcct gacccagagc gcgttcaccg gtctggccca gaccctttcg
     2521 tatcttgcgc ccacgctgct gacctcggtg gtctcacggc aggtgctcga tgcaaacggg
     2581 atcccggcgg tggtgcggat cgccttcgcg atcggggcgg tactctcgat ctcgaccatt
     2641 gtctggtcca tctggcgcgt gccggaactg ccgctggagc cggccgaggc ggcggcgctg
     2701 gcggcgaggc cgatgaccct gcgcgccacc ctggccgaga tcggcagcgc ctgtcgcgaa
     2761 atgccgccgg ccatgcgcca gctcgcgctc gccatgctgt gccagtggta tgcgatgttc
     2821 gcctactggc agtacatcgc ctttgccgtg ggccgctcgc tctatcacac gcacgacgcg
     2881 accagctccg ccttccgtga tgccacgctc accgcgcagc aggcaggggc gttctacaat
     2941 ctcgttgcct tcatcggcgc actggggctg atcccggtgg tccgccgcat cggcgcgcgc
     3001 cgggcccatg cgctgtgcct caccgcatcg ggcgccgcca tgctggcgat ttcgcacagc
     3061 ggcagcatgg ccgtgctgtt cgccgcgatg gtgggcatcg ggctgggctg ggcgggcatg
     3121 atgggcaata cctatgtcat gctggccgat gccatcccgc ccgagcgcaa cggcatctac
     3181 atgggcattt tcaacatgtt catcgtcatc ccgatgctga tccagtcgct gacgctgccc
     3241 ctcctctatg acggcctcct cggcgggcgg cccgagaacg tcatccggct ggccggcgtg
     3301 ctgctcggcc tggcagcgct gtgcgtgctg cgcgtcaaaa cctctgcttt gaaccagcga
     3361 tgaagaacca agtccaactc atcacctatg tcgaccgcct cggcgggcgg cgcgacggcc
     3421 gcgacc
//
LOCUS       DATNBV010000031         2319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_335217,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000031 DATNBV010000000
VERSION     DATNBV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335217"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..678
                     /locus_tag="VN627_00800"
     CDS             10..678
                     /locus_tag="VN627_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXD01822.1"
                     /translation="MPYDPIKDFVPITLVAGVPNVMVMQTEKARAMGINSVADFVRYA
                     KAHPGKLNMASSGNGTSIHLSGELFKSMTGVYMVHLPYRGSGPALMDMVGGTADVMFD
                     NLPSSMQQIKAGKLKALAVTSRQRSAALPDVPTVEEAAGLKGFDATSWFGLLAPAGTP
                     PDIVNRIQQEVAKSLNTAAIKEKLLSQGAIPSGNSPAEFARHIENEHRKWAQVVKASG
                     AKVD"
     gene            complement(753..1034)
                     /locus_tag="VN627_00805"
     CDS             complement(753..1034)
                     /locus_tag="VN627_00805"
                     /inference="COORDINATES: protein motif:HMM:NF020469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HXD01823.1"
                     /translation="MLEPNGRRVLQIIGKDAQPQGIILPEQIPAAVGALEAAIAREEA
                     DQKAMIEEAKAKGETPPRFDAISLRQRAVPFLDMLRRCEEAGEEIVWGV"
     gene            complement(1037..2167)
                     /locus_tag="VN627_00810"
     CDS             complement(1037..2167)
                     /locus_tag="VN627_00810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXD01824.1"
                     /translation="MAEDFVESRVQLEGRMRDLMAVAARLLPGNPVAGDTGWWTQMLR
                     RARSVAAHSPHKDAQQVQFHYDVSDDFYAIWLDPRRVYSCAYYRDTAMSLAQAQEAKL
                     DHICRKLQLAPGERFLDIGAGWGGLLMWAAENYGVNATGITLSKNQHAYVQRLIEEKR
                     LQGRVRMELRDYRELGETEGFDKIASVGMFEHVGQANMPAYFGKIHGLLKPGGLVMNH
                     GITAGGIHPGHMGAGMGEFIEKYIFPGGELLHISHVLKDMALAGLEMVDTENLRPHYA
                     RTLWAWSDALEARLDEAQRVLATTGHVDDAQKVLRAYRLYLAGCAMIFEQGWIGLHQI
                     LATRPDGQLHTGSLRGAQSAYPFNREYMYRCSSNSSPRQPAT"
BASE COUNT          426 a          764 c          714 g          415 t
ORIGIN      
        1 tacgacaagc tgccctacga cccgatcaag gatttcgtgc cgatcacgct ggtggccggc
       61 gtgcccaatg tgatggtgat gcagacggaa aaggcacgcg cgatgggcat caacagcgtt
      121 gccgatttcg tccgctacgc caaggcacat cccggcaagc tcaacatggc ttccagcggc
      181 aacggcacct cgatccatct gtcgggcgag ctgttcaaga gcatgaccgg cgtgtacatg
      241 gtccacttgc cctatcgcgg ctccggcccg gcattgatgg acatggtggg cggaacggcg
      301 gacgtgatgt tcgacaactt gccctcgtcg atgcagcaga tcaaggccgg caaactcaag
      361 gcattggcag tgaccagccg gcagcgctca gccgcactgc cggatgttcc gaccgtcgag
      421 gaggcggcgg gcctgaaagg gttcgacgcc acttcgtggt tcggcctgct cgcccccgca
      481 ggcacaccgc cggacatcgt caatcgaatc cagcaggaag tggccaagtc gctgaacacg
      541 gccgccatca aggaaaaact gctgtcgcag ggcgcgatcc cgagcggaaa ttctccggcc
      601 gaattcgcgc gccacatcga gaacgaacac cgcaaatggg ctcaggtggt gaaggcgtcg
      661 ggggcgaaag tggactgacg cccgttcgcc ctgagcttgt cgaagggcca gcgcacaaag
      721 gcttcgacag gctcagcccg aacggttggc gctcaaaccc cccagacgat ttcctcgccg
      781 gcttcctcgc agcggcgcag catgtccagg aacggcacgg cgcgctgccg caaggaaatg
      841 gcgtcgaaac gcggcggagt ttctcccttt gccttggcct cttcaatcat ggccttctgg
      901 tccgcctcct ccctggcgat tgcagcttcc agggccccca ccgcagcggg tatctgttcc
      961 ggcaggatga tgccctgggg ttgcgcgtcc ttcccgatga tctggagtac gcggcgcccg
     1021 ttgggctcca gcatgatcag gtcgccggtt gccttggact tgaatttgaa gagcatcggt
     1081 acatgtactc gcggttgaag ggataagccg actgggcgcc gcgcagcgag ccagtatgca
     1141 actggccgtc cggtcgcgtg gcgaggatct ggtgcagtcc gatccagccc tgctcgaata
     1201 tcatcgcgca gcccgccaga tacaggcggt aggcacgcag gactttctgc gcatcgtcga
     1261 catggccggt cgtggccagc acgcgctggg cctcatcaag tcgggcttcc agggcatccg
     1321 accaggccca caaggttcgg gcgtagtgcg gccgcaggtt ctccgtatcc accatttcca
     1381 gcccggccag cgccatgtcc ttgagcacgt ggctgatgtg gagcaactcc ccgccaggaa
     1441 agatgtattt ctcgatgaac tcgcccatcc ctgcgcccat gtggccgggg tggatgccgc
     1501 cggcggtaat gccgtggttc atgacgaggc cgcccggttt gagaaggcca tggatcttgc
     1561 cgaagtaagc cggcatgttg gcttgcccca cgtgctcgaa catgcctacc gacgcaatct
     1621 tgtcgaaccc ctcggtctcg ccgagttcgc ggtaatcgcg caactccatg cgcaccctgc
     1681 cctgcaatcg cttttcttcg atcagccgct gcacataggc atgctggttc ttcgacaacg
     1741 tgatgccagt ggcattgacg ccatagttct ccgccgccca catcagcagc ccaccccagc
     1801 ccgccccgat gtccaggaag cgctcgcctg gcgcaagctg aagcttgcgg cagatatggt
     1861 cgagtttcgc ttcctgggcc tgcgccagcg acatcgccgt atcgcggtag taggcgcacg
     1921 aatagacccg gcgcggatca agccagatgg catagaagtc gtcggacacg tcgtagtgga
     1981 actggacctg ctgcgcgtcc ttgtgtggag agtgagccgc cactgatctc gcgcggcgca
     2041 gcatttgggt ccaccagccg gtgtcgcccg ccaccgggtt gccgggaagc aagcgggccg
     2101 ccaccgccat caggtcgcgc atccgccctt ccagctggac ccggctctcg acgaaatcct
     2161 cggccatccg gccgatctgg cccgctgcca tagtggcgag gctggaccag tccttgaacg
     2221 acaaagtgac cgccgccatc ggcggtccca cccgcttgcc ggcgggcaac tgcaccgcca
     2281 ccggcacggg caagccggcc aattgcgact cgatcttgc
//
LOCUS       DATNBV010000032         3658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1615141,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000032 DATNBV010000000
VERSION     DATNBV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615141"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VN627_00815"
     CDS             complement(<1..264)
                     /locus_tag="VN627_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01825.1"
                     /translation="MQLSGRLGRKPRPVKAPRAARARHITWMLALPAGITRPMGAGAL
                     GDAALGRAGPTPVSQELPSMEPAGSAEPELLLRDEGPVRVLTLN"
     gene            288..1331
                     /locus_tag="VN627_00820"
     CDS             288..1331
                     /locus_tag="VN627_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXD01826.1"
                     /translation="MAQARMNYRRLGSTGLLVSELGLGTNTFGGKGEGFWEKLGALGQ
                     AEANKIVGRAFAAGINFIDTADMYGLGESEVIVGEAIRSLGCPRDEIVLATKIGAQFQ
                     RHVNGLGASRGHIMAAVDGSLKRLKLDHIDLLMMHFFDPATPLEETLGALDDCVRAGK
                     VRYIGCSNYTGWQVVKAVETSRRMGVNAFAVVETHWSAATREIERELLPAARSEGVGM
                     LIWGALLGGILSGKYADGAASSGRMAGIVPGTVNAEGLAKVVAILGEIGAKRRVAPAS
                     VALAWLLSHREVSSVLFGARTPEQVDENLRATELTLDADEIAAIDAAAPPSRDHFMMV
                     TRNSNLARLPYAR"
     gene            1371..2267
                     /locus_tag="VN627_00825"
     CDS             1371..2267
                     /locus_tag="VN627_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXD01827.1"
                     /translation="MQATAGSGIPVDSLWADAKIKGISVAVIGQARTAAAAVELHDVV
                     FHADAESDQLTANALFDLSLARRGGDPTGHYPDFHPSQRRLGELLFIPPRTRFHGQWQ
                     RCTQRTLWVIPTDDARWAHRDWSADLLAEALDIRSGELRGAMLRLANELANPGFASEL
                     MIEAISTEVLVLLQRHLLGPKGDGAAQRGGVLDRGQLRRIEDLADNPGPPPGIADLAS
                     ACGISERHFSRLFRLATGRSAAQFVAERRIERARDRLAGSRAPIKQIAWEAGFESPAA
                     FATAFRRATGQSPRAFRQAAQN"
     gene            2402..2926
                     /locus_tag="VN627_00830"
     CDS             2402..2926
                     /locus_tag="VN627_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01828.1"
                     /translation="MLPPFAGPALGNIMQVAWVPPDIERSMAMFGKTYRVPDWCVMEV
                     DFPAVAFGESGSMKLKIALANVDAIQLELIQPIGGGIDRIYRDALPADGSFANVFHHV
                     CVKILGPLENWEAYLATLGGERPIVYTGEIGENARFCYTDERATVGMFVEHVWFSPEQ
                     EAQMASVVPTYTTK"
     gene            2938..>3658
                     /locus_tag="VN627_00835"
     CDS             2938..>3658
                     /locus_tag="VN627_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXD01829.1"
                     /translation="MPKYSTRQHLVLPIVTWDEARPSEWINDHIAMVHATSNAYVVTG
                     DDGDVVINTGTAAQGAAIREKFEALIGRKLKVAKIVFTQSHPDHTGGWEAFADPGVEL
                     YGQRNFGQICAERHMLGGFFGPRNHRVLSAMMPPGTSHNWFSTPDPAPLTTFADEMEF
                     TCSGRTYRLITLSSGETLDSLAVWLPEEKTVFTGNWAGAVQRALPNFYTARGDRDRSI
                     PTWLADCDKLLALHPELLITGH"
BASE COUNT          640 a         1193 c         1201 g          624 t
ORIGIN      
        1 attgagggtc agcacccgca ccggcccctc gtcgcgcaag agcagttcgg gttcggcaga
       61 tcctgctggt tccatcgacg gcaattcctg cgacactggg gtgggcccgg cgcggcccag
      121 cgcggcatca cctagcgcgc ccgcacccat ggggcgagta attccggcag gcaatgccag
      181 catccacgtg atgtgacgcg cgcgcgcagc ccttggcgct ttgaccgggc gcggcttgcg
      241 gccaagcctg ccggaaagct gcacgaaccg gcaagaggat catggcaatg gcgcaggcgc
      301 ggatgaacta ccggcggctc ggcagcactg gcctgctggt gtcggaactc ggtcttggca
      361 ccaacacctt cggcggcaag ggtgagggct tctgggaaaa gcttggcgca ctcggccagg
      421 cagaggccaa caagattgtc ggcagggcct ttgcggccgg catcaatttc atcgataccg
      481 ccgacatgta cggactcggg gaatccgaag tcatcgtggg cgaggccatt cgcagccttg
      541 gctgccctcg cgacgaaatc gtgctggcca ccaaaatcgg cgcccagttt cagcggcacg
      601 taaacggact gggcgcatcg cgcgggcata tcatggcggc ggtcgacggc agcctgaagc
      661 ggctcaagct cgatcacatc gacctgctga tgatgcactt cttcgatccg gccacaccgc
      721 tggaagagac gctgggagcc ctggacgact gcgtgcgcgc cggcaaggtg cgctacatcg
      781 gatgttccaa ctacaccggc tggcaggttg tgaaggcagt ggaaacctct cgccgtatgg
      841 gagtgaatgc tttcgccgtc gtcgagaccc attggtcggc cgccactcgt gaaatcgagc
      901 gtgagctgct gcccgcagcg cgtagcgaag gcgttggcat gctgatctgg ggggctctgc
      961 tgggcggcat cctcagcggc aaatatgccg atggagcagc gtccagcggg cgcatggcgg
     1021 gcattgttcc cgggaccgtc aacgccgagg ggctcgcgaa ggtggtggcc atactcgggg
     1081 agatcggggc aaagcgccgc gtagctcccg catctgtcgc gctcgcctgg ctgctctcgc
     1141 accgcgaggt atcgagcgtg ctgttcggcg cccgtactcc cgagcaggtc gacgagaatt
     1201 tgcgggcgac cgaactcacg ctcgatgccg atgagatcgc cgcaatcgac gctgcagcac
     1261 cgccgtcgcg cgatcatttt atgatggtga cgcgaaattc gaatctggct aggctgccct
     1321 acgcgcggtg atctggccaa accggcaacc attcaggctg gagaaggacc atgcaggcga
     1381 ccgctggcag cggcatcccg gttgattcac tgtgggccga cgcaaagatc aaaggtatct
     1441 cggttgcagt aatcgggcag gcgcggactg ccgccgccgc cgtcgaactg cacgacgttg
     1501 tgtttcatgc cgatgcggaa tcggaccaat tgaccgcgaa cgctctattc gatctttcgc
     1561 tggcccgccg aggcggagat ccaacggggc attatcccga ttttcatccc tcccagcgcc
     1621 ggcttggcga actgctgttc attccgcccc gcacgcggtt tcatggacaa tggcagcgct
     1681 gcacgcagcg cactctctgg gtaatcccca ccgacgacgc acgctgggcg caccgcgact
     1741 ggtcggccga tctcctggca gaggccctgg atatccgcag cggtgaactg cgcggcgcga
     1801 tgctgcggct cgccaacgag ctggcgaatc ccggttttgc ctcagaactg atgatcgagg
     1861 cgatcagtac cgaagtcctc gtgctgctcc agcgccacct gctcggaccg aagggcgatg
     1921 gtgctgcgca gcgtggcggc gtgctggacc ggggccaact ccgccgcatc gaggaccttg
     1981 ccgacaatcc tgggccgccc cccgggatcg ccgatctggc tagtgcctgc ggaatttccg
     2041 agcggcactt cagccgcctg ttccgtctcg cgacgggccg ttccgcggcg cagttcgtgg
     2101 ctgaacggcg gatcgagcgc gcgcgggacc ggctggccgg gagccgggcc ccgatcaagc
     2161 aaattgcctg ggaggccggg ttcgagagtc cggccgcctt cgccacggcc ttccgccgcg
     2221 ccaccgggca atcgccccgt gcgttccggc aggcggccca gaactgacag cgatggcgca
     2281 atcggtgcgg ttctggccgg gccgagcaaa ttctccccct tgtgccatgg cgaaccgtcg
     2341 ttagcttgtc gcgattgccg ccggacgagc ggtgaccgac tctgggacaa ggattgcatt
     2401 gatgctccct ccattcgccg ggcctgcctt gggcaacatc atgcaggtcg cctgggtccc
     2461 gcctgacatc gagcgcagca tggccatgtt cggcaagacc taccgcgtgc ccgactggtg
     2521 cgtgatggaa gtggacttcc cggcggtggc gttcggagaa agcgggtcga tgaagctcaa
     2581 gatcgcgctg gccaacgtcg acgcgatcca gcttgagcta atccagccga tcggcggcgg
     2641 gatcgaccgg atctatcgcg acgcgcttcc tgccgacggc agcttcgcca acgtcttcca
     2701 ccacgtctgc gtcaagatcc tcgggccgct ggagaactgg gaggcctacc tcgcgaccct
     2761 gggcggcgag cggccgatcg tctacaccgg cgagatcggg gagaacgccc ggttctgcta
     2821 caccgatgag cgcgcgaccg tcggcatgtt cgtggagcac gtctggttca gcccggagca
     2881 ggaggcgcag atggcctccg tggtcccgac ctatacgacc aaatgacagg cgcaaggatg
     2941 cccaagtaca gcacgcgcca gcaccttgtc ctgccgatcg tcacctggga cgaggcgcgg
     3001 ccatccgaat ggatcaacga tcacatcgcg atggtccatg ccacctcgaa cgcctacgtg
     3061 gtcaccggcg atgacggcga cgtggtcatc aataccggca cggcggcgca gggcgcggcc
     3121 atccgcgaaa agttcgaggc gctgatcggc cgcaagctca aggttgcgaa gatcgtcttc
     3181 acccagagcc acccggacca taccggcggc tgggaggcgt ttgccgatcc gggtgtggaa
     3241 ctctatgggc agcgcaattt cggccagatc tgcgccgaac ggcacatgct cggcggcttc
     3301 tttggcccgc gcaaccatcg cgtgctttcg gccatgatgc cgccgggaac gtcgcacaac
     3361 tggttctcga cgccggaccc ggcgccgctc accacctttg ccgacgaaat ggagttcacc
     3421 tgctcgggcc gcacttaccg cctgatcacc ctgtcctcgg gcgaaacgct cgattcgcta
     3481 gcggtctggc tgccggagga gaagacggtg ttcaccggca actgggccgg cgcggtccag
     3541 cgggcgttgc ccaacttcta tacagcgcgg ggcgaccgcg accgctcgat cccgacctgg
     3601 ctcgccgatt gcgacaagct gctggcgctg caccccgagc tgctgatcac cgggcacg
//
LOCUS       DATNBV010000033         4956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_979142,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000033 DATNBV010000000
VERSION     DATNBV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979142"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..131
                     /locus_tag="VN627_00840"
     tRNA            58..131
                     /locus_tag="VN627_00840"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:90..92,aa:Gln,seq:ttg)
     gene            211..1791
                     /locus_tag="VN627_00845"
     CDS             211..1791
                     /locus_tag="VN627_00845"
                     /EC_number="3.4.11.19"
                     /inference="COORDINATES: protein motif:HMM:NF009622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminopeptidase"
                     /protein_id="HXD01830.1"
                     /translation="MTSPSAGLDRAIDALPRDYPGPGGAIAVLRKGEVLARHAWGWAN
                     AERRLAFTPRSLFRLCSITKQFTCGLILDAFPDPSVLDDDIRARLPLLEQAAPKALHL
                     CHNQSGLRDYWAVAMLQGAPVEAPFGEVEAACLMAAARKLQFAPGTRYSYCNQNFRIL
                     SDILQERTGRSFVELLRSRIFERVGMSNAFLAADTRAMPDGTEGYEGTQASGFRAAEN
                     RIIWTGDAALGACLDDMIAWERHIDAARDEPESLYRRLSVPVSFADGALAAYGFGLAR
                     STEFGRATTSHGGALRGWRSHRLHVRAERISVVVMFNHLSDARAAAVDLLAAVLGEER
                     PKPDAAIPAPSWLGSYLEPETGLAVRIAPGSAGRIKLRYGHSAEELDLRADGSASSAS
                     GTCLSFSDAGLWMSRPQDNQGSRLRPCDGGPTTDVAGRYRCGELDAELTVADTGGVLY
                     GAFSGLLGQGRMEMLDPVGPDVWTLPCHRALDHTPPGDWTLAFRRGQAGRVEGVDVGC
                     WLARRIPYERVAACRGAS"
     gene            1828..3414
                     /locus_tag="VN627_00850"
     CDS             1828..3414
                     /locus_tag="VN627_00850"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HXD01831.1"
                     /translation="MSYRVMAPEFETLVDQNEAIRTVAGGFIFTEGPIWHPTGHYLLF
                     SDMPGDVRRRWDAEDGVREVRRPANKCNGMTYDADLNLIVCEHATSSLIRETPAGGRQ
                     VLASHFEGQELNSPNDVCVRSDGSIYFSDPWYGRMPGYGVERDRQLGWQGVFRLTPDG
                     KLHLVVPRHDFAMPNGLCFSPDEKLLYINDTENGLIRVYEVAANGVLRHGRIFAEGMI
                     SQKEAGVPDGMKCDERGNIWCTGPGGVWVFSPEAELLGRIAVPEVVGNLHWGGADFKT
                     LFLAASTSIYSLRTKVGPRPEPFMHKRRAPAAGKQTGAKQESEAEAETGSLALDPARC
                     ALIIQDLQNDVISEGGAFASSGAPGHATRQKIVQNVGRLARACRSLGIPVIHVWFVVD
                     PGAPGMTLNAPLFQSVRGSEAMIRGSWGAAPVAGLEPEPGDFVVEKMRMSAWEGTRLE
                     TILKALNRDVIINTGAWTNMSVEHTARTGADKGYVMIVPEDACSTVDEAWQRASIDFA
                     LRNVATITTCRALEEVMASQ"
     gene            complement(3440..3706)
                     /locus_tag="VN627_00855"
     CDS             complement(3440..3706)
                     /locus_tag="VN627_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD01832.1"
                     /translation="MTKALAVELAPKIRVNVVCPGPVATPMMEAEMDAYPDPVAARKE
                     SFERVPLKRWATAEEVARAILFLAADAPYATGASLALDGGTTAI"
     gene            complement(3703..4200)
                     /locus_tag="VN627_00860"
     CDS             complement(3703..4200)
                     /locus_tag="VN627_00860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD01833.1"
                     /translation="MGADTGRLNGMRTFITGASSGIGEATARLFAAEGARVALVARRR
                     DVLGPIARAIGERALALPADVADSRQVERAVDAAVEAFGGIDIVVNAAGVYSPVALRE
                     LTPAVWRELIDINLSGTYYVAREAALRMLDADGGAIINIGSELSHIGMALSSHYCASK
                     AGCSD"
     gene            4462..>4956
                     /locus_tag="VN627_00865"
     CDS             4462..>4956
                     /locus_tag="VN627_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXD01834.1"
                     /translation="MGAFSSISRRRLIKSTAALAALGALPMRKGFAAGTELKILNCNV
                     AWSAALGDAVAKAYQQKTGTTVTAEPTPYDSLYQKILVELSQGSSTYDLVTSDELWMR
                     QPINNGWAPALEDIKAKNPALPDMHYDNLVPQSLLYTDVGGKHYGLPMTMSTPVFIYR
                     KDLFE"
BASE COUNT          837 a         1657 c         1629 g          833 t
ORIGIN      
        1 ctggtcgccc tcttcgccga ccgccgctga cccgacctat actcgtcagc ctgactctgg
       61 ggcgtggcca agtggtaagg caacgggttt tggtcccgtg atcccaggtt cgaatcctgg
      121 cgccccagcc aacaatgaca gaaaatatag ccggatcagt tgcttcggca gttgccctcc
      181 gcgagcgaag acgcgagagt gccggccatg atgacctcgc cctccgccgg ccttgaccgc
      241 gctatcgacg ccctgccccg cgactacccc ggacccggtg gtgcgattgc cgtattgcgc
      301 aagggcgagg tgctggctcg tcacgcctgg ggctgggcca atgccgagcg gcgccttgcc
      361 ttcacgccgc gctcgctgtt tcgcctgtgc tcgatcacca agcagttcac ctgcggcttg
      421 atcttggacg cctttcccga cccgtcggtg ctcgatgacg acatacgcgc ccgcctgccg
      481 ctgttggagc aggctgcgcc aaaggcattg cacctttgcc acaaccagtc gggcctgcgc
      541 gactactggg ccgtggcgat gctgcagggt gcgcctgtcg aggcgccgtt cggtgaggtg
      601 gaagccgcct gcctcatggc cgccgctcgg aagctccagt ttgcgcccgg cacccgctac
      661 tcctactgca accagaattt tcggattctg tcggatatcc tgcaggagcg cacgggacgg
      721 agcttcgtgg agctgttgcg cagccgtatt ttcgagcgcg tcgggatgtc caacgccttc
      781 ctggccgccg acacgcgcgc catgccggat gggacggagg gctacgaggg tacgcaggcc
      841 agtggtttcc gcgccgcgga aaatcgcatc atttggacag gcgatgccgc gctcggcgcc
      901 tgcctcgacg acatgatcgc ctgggagcgg catatcgatg cggcccggga cgagcccgag
      961 tccctgtatc ggcggctgtc ggtaccggtc agctttgccg atggggcctt ggctgcctat
     1021 ggcttcggct tggcccggtc gaccgagttc ggccgggcga ccaccagcca tggcggcgcg
     1081 ctgcgcggtt ggcgcagcca tcgtctccat gttcgcgcgg agcggatctc cgtcgtcgtc
     1141 atgttcaatc acctctccga cgcacgggcc gcggcggtcg acctgctcgc cgccgtgctg
     1201 ggcgaagagc ggccgaagcc cgatgccgcc atcccggcgc cgagttggct cggcagctat
     1261 ctggagccgg agacggggct tgcggtacgc atcgcaccgg gatcagccgg acggatcaag
     1321 ctccgctacg gtcactctgc ggaagagctt gacctccggg ccgacggttc ggcgagcagc
     1381 gcctctggaa catgcttgag cttcagcgac gccgggctgt ggatgagccg cccgcaagac
     1441 aaccaaggct cgcgcctgcg cccctgtgac ggcgggccca ccaccgatgt cgccggccgc
     1501 taccggtgcg gagagctcga tgccgagctc acggtggcgg acaccggcgg cgtgctctat
     1561 ggcgccttct cggggcttct gggccagggg cgaatggaga tgctcgatcc ggtcgggccg
     1621 gatgtctgga ccttgccctg ccaccgtgcg ctcgatcaca cgccgcccgg cgactggacc
     1681 ttggcctttc gacgcggcca ggccggccgg gtcgaaggcg tcgatgtcgg ttgctggctc
     1741 gctcgccgaa ttccctacga gcgcgtagca gcctgccggg gagccagtta aagtcaaaat
     1801 agatgcgagg agcccgggag gggagcgatg tcctatcgcg tgatggcgcc ggaattcgag
     1861 accctggtgg accagaacga ggcgatccgg accgtggccg gcgggttcat cttcaccgag
     1921 gggccgatct ggcacccgac cgggcattat ctcctgttct cggatatgcc gggcgacgtc
     1981 cggcggcgat gggatgccga ggacggcgtc cgcgaggtcc gccgccccgc gaacaaatgc
     2041 aacggcatga cttatgatgc cgatctgaac ctgatcgtct gcgagcatgc aacctcctcc
     2101 ctcatccgcg agactcccgc cgggggccgc caggtcctgg cctcgcattt cgaggggcag
     2161 gagctcaaca gccccaatga cgtctgcgtc cgatcggacg gatcgatcta tttcagcgac
     2221 ccctggtacg gccgcatgcc gggctatggc gtcgagcgcg atcgccagct cggctggcag
     2281 ggggtgtttc gcctcacccc ggacggcaag ctgcatctcg tcgtcccgcg ccacgatttc
     2341 gccatgccca acgggctctg cttctcgccc gacgagaagc tcctctatat caacgatacc
     2401 gagaacgggt tgattcgcgt ctatgaggtg gcggcgaacg gcgtgctgcg gcatggccgg
     2461 attttcgccg aggggatgat ctcgcagaag gaagccgggg ttcccgacgg catgaaatgc
     2521 gacgagcggg gcaatatctg gtgcaccgga ccgggcggtg tctgggtctt ctcgcccgag
     2581 gcggagctgc tgggccggat cgccgtgccc gaagtggtcg gaaatcttca ctggggcggg
     2641 gccgatttca agacgctgtt tctggcggcc agcacctcga tttacagcct tcgcaccaaa
     2701 gtcggcccgc gtcccgagcc cttcatgcac aagcggcgtg cacccgccgc cggcaagcag
     2761 actggcgcga agcaggagag cgaggcagag gccgagactg ggagtctggc gctcgatccc
     2821 gcccgctgcg ccctgatcat ccaggatctg cagaacgacg tgatcagcga gggcggcgcc
     2881 ttcgcgagct ccggggcgcc gggccatgcc acccggcaga agatcgtcca gaatgtgggc
     2941 cgcctcgcgc gggcctgccg ctcgctgggt atccccgtga ttcatgtctg gttcgtggtc
     3001 gatcccggcg cgccgggcat gacgctcaac gcccctctct tccagagcgt tcgcggcagc
     3061 gaggcgatga tccgcggcag ctggggggcg gcgccggtcg cggggctcga gccggagccc
     3121 ggcgatttcg tggtcgagaa aatgcgcatg agcgcctggg aaggcacgcg gctggagacc
     3181 atcctcaaag cgctgaaccg cgatgtaatc atcaataccg gcgcctggac gaacatgtcg
     3241 gtggagcaca cggcccgcac cggggcggac aaaggctatg tcatgatcgt gcccgaggac
     3301 gcctgctcca cggtggatga agcctggcag cgcgcctcga tcgacttcgc cctcaggaat
     3361 gtggcgacga ttaccacttg ccgcgcgctg gaagaggtga tggcaagcca gtgaagatcg
     3421 tcaattccgg caccggcgtt caaatcgcgg tggtgccgcc gtcgagcgcg agagaggccc
     3481 cggtcgcata gggcgcgtcc gccgccagaa acaggatcgc gcgagcgacc tcttccgctg
     3541 tcgcccatcg cttcagcgga acccgctcga aggattcctt gcgggctgcg acgggatccg
     3601 gataggcgtc catctccgcc tccatcatcg gggtggccac cggccccggg cagaccacat
     3661 tcaccctgat tttcggcgcc agctcgaccg ccagggcctt ggtcaatccg agcaccccgc
     3721 cttcgaggcg cagtagtggc tgctgagcgc catgccgata tgggaaagct ccgagccgat
     3781 gttgatgatc gccccgccat cggcgtcgag catgcgaagc gctgcctcgc gggcgacgta
     3841 ataggtgccg gagaggttga tgtcgatcag ctcccgccag accgcgggcg tcagctcgcg
     3901 caaggccacg ggcgaataga cgcccgccgc gttcacgacg atatcgatgc cgccgaaggc
     3961 ctcgacggcc gcatcgaccg ccctctccac ctgccgcgaa tcggcgacat cggccggcaa
     4021 tgccagcgcc ctctcgccaa tggcgcgggc gatcggcccc agcacgtccc ggcgccgcgc
     4081 caccagcgcc acccgggcgc cctcggccgc gaacaaccgg gccgtggcct cgccgattcc
     4141 cgaggaggcg ccggtgatga aggtccgcat gccattgagc ctgcctgtgt ccgcgcccat
     4201 ctctgcctcc cccaattctg ccgccccgcc ggcgacgctc gcaaggcgcc ggttgccgcc
     4261 gtcgccccat tagagcaggg tgcgcgcttc ttgtcgaatg actgtcgaca gaaattgggc
     4321 atcgtgtaat atattcgtat gcagagaccc gaacgggcat aaagcctgcc ggtggacgaa
     4381 tgccggccgc aagccgcctt cgaccgtcgc gagaccacaa gaaacaacca gatatcaaac
     4441 agcaggacag gagcatcaca gatgggagcg ttcagttcga tctcgcggcg ccggctgatc
     4501 aaatcgacgg cggcgctggc ggcactcggc gccttgccga tgcgaaaggg atttgcggcg
     4561 ggcaccgagc tcaagatcct caattgcaat gtcgcctggt cggcagccct gggtgacgcc
     4621 gtggcgaagg cctatcagca gaagaccggg acgaccgtca cggcggagcc gacgccctac
     4681 gattccctct atcagaagat actcgtcgag ctcagccagg gatcgagcac ctatgatctc
     4741 gtcacgagcg acgagctgtg gatgcgccag cccatcaaca acggctgggc gcccgcgctc
     4801 gaggacatca aggccaagaa tcccgcgctg cccgacatgc attacgacaa tctcgtgccg
     4861 caatcgctgc tctataccga tgtcggcggc aagcactacg gactcccgat gaccatgtcc
     4921 acgccggtgt tcatctatcg caaggatctg ttcgag
//
LOCUS       DATNBV010000034         2715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3849833,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000034 DATNBV010000000
VERSION     DATNBV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3849833"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VN627_00870"
     CDS             complement(<1..1074)
                     /locus_tag="VN627_00870"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HXD01835.1"
                     /translation="MANVTVIGAQWGDEGKGKIVDWLASRADAVVRFQGGHNAGHTLV
                     VGDTTYKLSLLPSGIVTGTLSIIGNGVVLDPWHLRDEIAKLEGQGVVINTENFAIAEN
                     APLILPLHRDLDGLREAAAGSGKIGTTGRGIGPAYEDKVGRRAIRVCDLAHLDALDAQ
                     LDRLCAHHDALRAGFGQPPVDRAKLLEELREIAPSVLKYAQPVWKRLNTVKKAGARIL
                     FEGAQGVLLDVDHGTYPFVTSSNTVSGTAASGSGLGPNATGFVLGIVKAYTTRVGSGP
                     FPTELDDATGQRLGERGHEFGTVTGRKRRCGWFDAVLVRQSCAISGVTGIALTKVDVL
                     DGLETVKICTGYRLGGKVLDYFPS"
     gene            complement(1200..2180)
                     /locus_tag="VN627_00875"
     CDS             complement(1200..2180)
                     /locus_tag="VN627_00875"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXD01836.1"
                     /translation="MRWKNRPEGSNWGDFGPDDQVGRLNLITPERRRAAAAEVREGLA
                     FNLSLPLDYPRGQLIGFRNPPRLFTTSGAHGHPLRDDHVDICCDDGVTLALQFSSQWD
                     ALAHMGAAFDADGDGEAERVFYNGFRAGADVSSDSEGVPRVRHLGIETLSQTCVQGRG
                     VMVDLHREAGRNHAAVGYDRLMRTMEAQGATVEPGDMLCLYTGFDRVILDLGEQITPA
                     HAHTTCASLDAGDSRLLRWIDECGLVALISDNMAIEGFGPNAVADGDVVLPIHHLCLF
                     KLGIPLGELWYLEELNRWLAAHGRTRFLLTAPPLRLPGAVGSPATGVATV"
     gene            complement(2268..>2715)
                     /locus_tag="VN627_00880"
     CDS             complement(2268..>2715)
                     /locus_tag="VN627_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HXD01837.1"
                     /translation="AIDAGGDLAPRIAALREIAARVGARARLTLDPSERHGFEYQSWF
                     GFTLYADGMSGALGRGGTYAILGKAPGEDEPAVGFSLYPDALIDLIAAAEAPRDILFL
                     PLGHDAEVAARLRAIGWRTVAALTAADDAGALGCTHRLDGNDAVRL"
BASE COUNT          432 a          868 c         1007 g          408 t
ORIGIN      
        1 gctggggaag tagtccagca ccttgccgcc caggcgatag ccggtgcaga tcttcaccgt
       61 ctcgaggcca tcgagcacgt cgaccttggt cagcgcaatg ccggtcacgc cggagatggc
      121 gcaggactgg cgcaccagca ccgcgtcgaa ccagccgcag cggcgcttgc ggccggtgac
      181 cgtgccgaac tcatgtccgc gctctccaag gcgctgcccc gtcgcatcgt cgagctcggt
      241 cgggaacggg ccggagccga cgcgggtggt ataagccttg acgatgccga ggacgaagcc
      301 ggtggcattg gggccgaggc ccgagcccga ggcggccgtg ccgctcaccg tgttggagct
      361 ggtgacgaag gggtaggtgc cgtgatcgac atcgagcagg acgccctggg cgccctcgaa
      421 caggatgcgg gcgccggcct tcttgaccgt gttcaggcgc ttccacaccg gctgggcgta
      481 tttcagcacg gacggggcga tctcgcgcag ctcttcgagc agcttggcgc gatcgaccgg
      541 gggctggccg aagccggcgc gcagggcatc gtggtgggcg cagaggcgat cgagctgggc
      601 gtccagcgca tcgaggtggg caaggtcgca cacccggatg gcgcggcggc cgaccttgtc
      661 ttcataggcg gggccgatgc cgcgcccggt ggtgccgatc ttgcccgagc cggcggcggc
      721 ttcgcgcagg ccatcgagat cgcggtgcag cggcaggatc agcggggcgt tttcggcgat
      781 ggcgaagttt tcggtgttga tgaccacgcc ctggccttcg agcttggcga tctcgtcccg
      841 caggtgccac ggatcgagca ccacgccgtt gccgatgatc gacagcgtgc cggtgacgat
      901 gcccgagggc agcaggctga gcttgtaggt ggtgtcgccc acgaccagcg tatgcccggc
      961 gttgtgaccg ccctggaagc ggaccacggc gtcggcgcgg ctggccagcc agtcgacgat
     1021 cttgcccttg ccctcatcgc cccactgggc gccgatcacg gtgacgttgg ccaaggtttc
     1081 ctgctctttc tattgcactg aagtgtccgc gccgcgccct aggggagagt ggccgcaagg
     1141 gcaaggtgag tcgggcccgg cgggcccggg ccgcacggca gtggcgctcg agcgtgccgc
     1201 tagaccgtcg ccacgccggt tgccggggag ccgacggcgc cgggcaggcg cagcggcggg
     1261 gcggtgagga gaaagcgggt ccgcccgtgg gcggccagcc agcggttgag ctcttccagg
     1321 taccacagtt cgcccagcgg aatgccgagc ttgaacaggc agaggtggtg gatcggcagg
     1381 acgacatcgc catcggcgac cgcattggga ccgaagcctt caatcgccat gttatcggaa
     1441 atgagcgcaa ccagcccgca ctcgtcgatc cagcgcagca ggcgggaatc gccggcatcg
     1501 agcgaggcgc aggtggtgtg ggcatgggcg ggcgtgatct gctcgccaag gtcgaggatc
     1561 acccggtcga agccggtgta gaggcacagc atgtcgcccg gctcgaccgt ggcgccctgg
     1621 gcttccatcg tgcgcatcag ccggtcgtag ccgacggcgg catggttgcg cccggcttcg
     1681 cgatggagat cgaccatcac gccgcggccc tggacgcagg tttgggacag ggtctcgatg
     1741 ccgagatgac gcacgcgggg aacgccctcg gaatcgctgc tcacatccgc tccggcgcga
     1801 aaaccattgt agaagaccct ctcggcctcg ccatcgccat cagcgtcgaa ggccgcgccc
     1861 atgtgagcca gggcatccca ctgggaggag aactggaggg ccagggtgac gccgtcatcg
     1921 cagcagatgt cgacgtgatc gtcgcgcaag ggatggccat gggctccgct ggtggtgaac
     1981 agccggggcg gattgcgaaa gccgatcagt tgcccacgcg gatagtccag cggtaggctg
     2041 aggttgaagg caagaccctc ccgcacttcc gcagcggcgg cgcggcggcg ctcgggcgtg
     2101 atcaggttga ggcggccgac ctgatcatcg gggccgaaat ccccccagtt ggaaccttcc
     2161 gggcgattct tccaccgcat atgcctctcc ttgccggtgc cgccttgcgg gcgcctggca
     2221 acgactgttc ggacaagaac agcgacaagg caatcacttg ctgccgctca gagccggacg
     2281 gcgtcgtttc cgtcgagccg gtgggtgcag ccgagcgccc ccgcatcgtc ggcggcagtc
     2341 agcgccgcga cggtgcgcca gccgattgcc cgcaggcggg cggcgacttc ggcatcgtgg
     2401 ccaagcggca ggaacaggat atcgcgcggc gcttcggcgg cggcgatgag gtcgatcagc
     2461 gcgtcggggt agagcgagaa gccgacggcc ggttcgtcct cgccgggggc cttgccgagg
     2521 atggcatagg tgccgccgcg cccgagcgct ccggacatgc catcggcgta gagggtgaag
     2581 ccgaaccacg actggtattc gaagccatgc cgctcggacg ggtcgagggt gaggcgcgcg
     2641 cgggcgccga cgcgggcggc gatttcgcgc agggcggcga tgcgcggggc gagatcgccc
     2701 ccggcatcga tcgcg
//
LOCUS       DATNBV010000035         3212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1615858,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000035 DATNBV010000000
VERSION     DATNBV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615858"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1140)
                     /locus_tag="VN627_00885"
     CDS             complement(109..1140)
                     /locus_tag="VN627_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01838.1"
                     /translation="MWRKIRRRPFERLVFAGQTLPGPVSGPLSGPLPRIQPVDRGLGL
                     FAPAVPGCLGALAGAGIGARAEALGLGADMVGPGHGEERIGRAGPGRGPVGHGGEARG
                     LLAGKHGQQLVEPRGPGGGKRIALGRAVEPGEMGEQEAAVVAAAAGDLLAMVEHRLLH
                     AVQRALGQHGLGMGAGEDERRIPPGGEARAAAAADIGRLRRQGDPRTGRADRAGHGEV
                     MEEAGLALGGEAVVAGFDLVRGGGRGFTPFGFAEGELTLRARHRGAGRGGGGRKILPT
                     RGEGDRRNGGGGGSPPQRGARREAPSVSGQAAATSPRVGRIAVIRQANRPLGGNPAAA
                     EGAGGGVVG"
     gene            1256..1828
                     /locus_tag="VN627_00890"
     CDS             1256..1828
                     /locus_tag="VN627_00890"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXD01839.1"
                     /translation="MSRILGQPVHVCYVYPDFDAALERFAQGGIGPFFVMHSGDSSGR
                     YRGQDLPLNISVAFFYTGDMCIEVITLNGPQTNAYSEFIERNPTGGLHHMAYMSDDFD
                     ATLARMAAAGRPLRVVQEYFNEGDAEPFEIYCEPEGEADPIMYQLMRPGLFDAWFDAM
                     KAAADDWDGSEPIRDAYALMMEARTAAAAG"
     gene            1956..2378
                     /locus_tag="VN627_00895"
     CDS             1956..2378
                     /locus_tag="VN627_00895"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HXD01840.1"
                     /translation="MIHKAFVAATGAAALLAAGPALAAPTKLTATLTGAAETPPGKPG
                     GTGGFTVTVDPETNDFCYSLWADGIGKPTMAHLHSGAAGASGPPVITLDVTGKDDDEC
                     LAVDKEKLDPIVANPSAYYVNVHTAEFPGGAVRGQLAK"
BASE COUNT          444 a         1202 c         1091 g          475 t
ORIGIN      
        1 cggccccgct cccggccccg gcgcgggcct caaacctttc ctacaccccc aacctgacat
       61 acctgaaacg ctccccttcc tccgcccagc gggagcacca tcatgtcatc acccgacaac
      121 accccctcca gccccttcgg ccgcggctgg attcccgccg agcgggcgat tggcctgccg
      181 gatgacggca atcctcccca cgcgtgggga ggtggcagcg gcctggccgc tgacggaggg
      241 ggcttcccgg cgggcgccgc gctgcggggg agagccccct ccaccgccgt tccggcggtc
      301 cccctcccca cgagtgggga ggatcttcct tcctcctccg cccctccctg ctcctctgtg
      361 tcttgcccgg agggtaagtt caccctcggc gaagccgaag ggcgtgaacc ccctgcctcc
      421 gccgcgcacc aggtcgaacc ccgccacgac cgcttcaccc cccagcgcca ggcccgcttc
      481 ctccatcacc tcgccgtgtc cggctcggtc cgcgcggcct gtgcgcgggt cgccctgtcg
      541 ccgcaggcgg cctatgtctg ccgccgccgc tgcccgcgct tcgccgccgg gtgggatgcg
      601 gcgctcgtcc tcgcccgcgc ccatgccgag gccgtgctgg ccgagcgcgc gctggacggc
      661 gtggaggagg cggtgttcta ccatggcgag gagatcgccc gccgccgccg ctacgacagc
      721 cgcctcctgc tcgcccatct cgcccggctc gaccgcgcgg ccgagcgcga tccgcttgcc
      781 gccgcccggg ccgcgcggtt cgacgagctg ctggccatgc ttgccggcga ggagcccgcg
      841 ggcctcgccc ccgtgcccga ctggaccccg gccggggccc gcgcggccga tccgttcctc
      901 cccgtgcccc gggccgacca tgtccgcgcc gagaccgagg gcctcggccc gcgcgcctat
      961 ccccgcgccc gcaagcgcgc cgaggcagcc tgggaccgct ggcgcgaaca ggccgaggcc
     1021 gcggtcgacc ggctggatgc gggggagggg gccggagagg gggccggaga cgggaccggg
     1081 gagggtctgc cccgcgaata caagtcgctc gaagggccgg cggcggattt tccgccacaa
     1141 ccgtgtcaac ttcgtcaatc tggccgctgc gatggacagc gcgggcgccc cgtgacaacg
     1201 tgcccgccat ggccggcgcg cagcctggtg aggcgccggc gcgggagaac aggtgatgag
     1261 caggatcctc ggccagccgg tccacgtgtg ctatgtctat ccggatttcg atgccgcgct
     1321 cgagcgcttc gcccagggcg ggatcgggcc gttcttcgtc atgcattcgg gcgacagttc
     1381 cggccgatac cgcgggcagg acctgccgct caacatctcg gtcgcctttt tctacacggg
     1441 cgacatgtgc atcgaggtaa tcacgctcaa cggcccgcag accaacgcct attccgaatt
     1501 catcgagcgc aatcccaccg gcgggcttca ccacatggcc tacatgtcgg atgatttcga
     1561 cgccacgctc gccaggatgg ccgccgcggg ccggccgctc agggtggtgc aggaatattt
     1621 caacgagggc gatgccgagc cgttcgagat ctactgcgag cccgagggcg aagccgatcc
     1681 catcatgtac cagctgatgc gcccgggcct gttcgatgcc tggttcgatg ccatgaaggc
     1741 ggccgcggac gactgggacg gcagcgagcc catccgcgat gcctatgccc tgatgatgga
     1801 agcgcggacg gcggcggcgg cggggtagcg gggcagcggg ccgggcaggg cgcctgcttg
     1861 ccggggcgcg gcctcgggta taggctgatg cgcaaggcag gcggcggggg gcgcgcgatc
     1921 ctcccggctt ggcctccgag cgaggagaga tgtccatgat ccataaggct tttgtcgctg
     1981 caaccggcgc cgccgcgctg ctcgccgccg ggcccgcgct ggccgcgccg accaagctca
     2041 ccgccaccct caccggcgcg gccgagaccc cgccgggcaa gccgggcggc accggcggct
     2101 tcaccgtcac cgtcgatccg gaaaccaacg acttctgcta ctcgctctgg gccgacggga
     2161 tcggcaagcc gaccatggcc cacctccact ccggcgccgc cggcgccagc gggccgccgg
     2221 tgatcacgct cgacgtcacc ggcaaggacg acgacgaatg cctcgcggtc gacaaggaga
     2281 agctcgaccc gatcgtcgcc aacccctcgg cctactacgt caacgtccat accgccgagt
     2341 tccccggcgg cgcggtgcgc ggccagctgg cgaagtaggt aggttgcgcg gagcaacgga
     2401 ttgccccgtc gcgggcggcg gatgacaaat ggggccggcc tcgcggccag ccccattctc
     2461 gcctgccggt cgctgcctgg cgaagccgat cccgtggcac tggccttgcg gtccgtgtcc
     2521 ggtcatcggg aagcgcgcgg tcgcggtgat gcctgccgag cgccccgacc gtggcaccgg
     2581 ggggtgcttc gcatcgtgat gctcgtcgca tcggacccgg ccggtttcga cctgcgccct
     2641 gcggcgccgc cttcccccgc cgttgctcgc ctcggcgctc cggtggtgcc cggtgcgccc
     2701 cgccatctgc cggagcccgc gaccggatgc ggcaagccgc ggtccggcca ggctcccggc
     2761 ccggcgtccg gtcccgtcag gcggggctgg gatgactgga aggatgaccc gatggtcatc
     2821 tccgtcaccg tcgccgtccg cccggcggtt ccgtgagcgt tcgtgccggc ctcggcccgc
     2881 tgcccttgcg accttcgcgt cacccaccct tgcgggcagt gccgtgcgac catcgggcag
     2941 gcaggatcgg cctgcaggaa cgttcatccg gcaatggtat cgccggaacg agaacatcgg
     3001 attgtcaggg gtttcggagg ctcgctcgta ccacccgttt gcacatccgc tgcttcgata
     3061 aagaaaggct gcgcccgtcc cctgagtcgc acaagaagtt tcagatgcaa ttacccacag
     3121 ctccccgacg caccggtgga taagtgcgcc gaagcggtgg acaagttgcg taaggttagc
     3181 agtcgcgttg agcgagcagg cgcaacctga gg
//
LOCUS       DATNBV010000036         3731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_337544,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000036 DATNBV010000000
VERSION     DATNBV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337544"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..890
                     /locus_tag="VN627_00900"
     CDS             267..890
                     /locus_tag="VN627_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HXD01841.1"
                     /translation="MDAAADVLASRPESEICLQCGLCCTGAIFDYAPLTAAELPFLVS
                     GKFARWQDKAGFAFPCSQLDGACCTSYHDRPSVCRRYRCEVLVRLADGRLTRDDAARQ
                     VGRAGQLVNSALAAARDRSATIGELRRAFQSGQDGWAEAGPDERAALAPLRLALAALE
                     RHLDGHFRRPHQRQMAELPGATEASPASAEQPCSPPALRRARAVNRR"
     gene            complement(877..2283)
                     /locus_tag="VN627_00905"
     CDS             complement(877..2283)
                     /locus_tag="VN627_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXD01842.1"
                     /translation="MTFKGLQPILYGGREVWPLVEGGKGVAATNHLSSGAWAAAGGIG
                     TVSAVNADSYDAEGKIVPQVYEQLTRKERHEQLIRYAIDGAVEQVRRAYDLASGKGAI
                     NINVLWEMGGAQQVLEGVLEKTRGLVTGVTCGAGMPYKLSEIAARFNVNYLPIVSSGR
                     AFRALWKRAYHKVADLMAAVVYEDPWLAGGHNGLSNAEDPRKPEDPYPRVKALRDTMR
                     AEGVADSVPIVMAGGVWFLREWNDWIDNPELGAIAFQFGTRPLLTAESPIPQGWKDHL
                     RTLEPGDVLLHRFSPTGFYSSAVRNPFLRALEGRSERQIPYSKVAAGEHTVQLDVGVK
                     GKNFWVAPADLDRARSWFGAGYVHGLRTPDDTIVFVSPEERSEIQQDQKDCMGCLSHC
                     GFSSWKDHDDYTTGRLADPRSFCIQKTLQDIAHGGDIDKNLMFAGHAAYRFKQDPFYS
                     NNFTPTVKQLVDRILTGD"
     gene            complement(2291..3091)
                     /locus_tag="VN627_00910"
     CDS             complement(2291..3091)
                     /locus_tag="VN627_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXD01843.1"
                     /translation="MRPRRRLALALLVLAAALQVGCSAPVDRDAGRSPEARGFPRADR
                     PVSRIISTQFSTEDQRDRLGEARLVMEWSHIEPGMTLADIGAGEGYYTVRLAEKVGKK
                     GRVLAEDIDPGALERLGRRIEKERLDNVSIKLGTPDNPRLPQASFDRVLMVHMYHEVA
                     EPYAFLWHMRPALKAGGSVVVVDADRPTDQHGTPPDLLFCEFGALGFRLAAFARNPGI
                     SGYYARFEPAGPRPAPAAIRPCRLAPDAAGGRLVANAAGVSGEEPGGK"
     gene            complement(3091..>3731)
                     /locus_tag="VN627_00915"
     CDS             complement(3091..>3731)
                     /locus_tag="VN627_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HXD01844.1"
                     /translation="LDLQAGDEAGIKSVTLGIRGENAYGYLRTERGVHRLVRISPFDA
                     QSRRHTSFASLDVVPEVAGDVEVQIEDKDLRVDTYRSSGAGGQHVNTTDSAVRITHVP
                     SGIIVASQIDRSQHKNREIAMNMLKARLYEAEMQRREEAASAEHASKSDIGWGHQIRS
                     YVLQPYQMVKDLRTGVTSPTPSDVLDGALDPFMAAALAQRVTGEKVAVEDVD"
BASE COUNT          598 a         1197 c         1297 g          639 t
ORIGIN      
        1 gtgctggcgc tcgccgtcaa cctggccgtg ccgatgcggg tgtcggagga cgaggagcga
       61 gaaggcctcg acctcaccag ccacggcgaa cgcgcctggg aaatggactg atcctgaaat
      121 gaagcggcag aaacgcgcaa ataaatttgc gcgtttctgc cgggagttaa ctgtcgcatt
      181 tcaggacagg agcggtatcg ccgccgcagc cccctttact gccgtaatcg attgtttacc
      241 atcatcgccg tctgggggga cggtcgatgg atgctgctgc agacgtactg gcatcgcggc
      301 cggaaagcga aatctgcctg caatgcggcc tgtgctgcac gggcgcgatc ttcgattatg
      361 ccccgctgac ggcagcggaa ctgccatttc tcgtctccgg caagttcgcc cgctggcagg
      421 acaaggcagg cttcgccttc ccatgctcgc agctcgatgg cgcgtgctgc acatcctacc
      481 atgaccggcc gagcgtgtgc cgccgctatc gctgcgaggt gctggtgagg ctggcggacg
      541 gcaggctgac gcgggacgat gccgctcggc aggttgggcg cgccggccag ctggtgaatt
      601 ccgcgctggc cgcggcgcgc gaccggtccg ccacgatcgg cgagctgcgg cgggcgtttc
      661 aatccgggca ggatggctgg gccgaggccg gtccggacga gcgggccgcc ctcgctccgt
      721 tgcggcttgc cctggcagcg ctggagcgcc acctcgacgg tcacttccgc cggccgcacc
      781 agcggcagat ggccgaactg cctggggcaa cggaggcatc accggcgagc gcggaacagc
      841 cttgcagccc ccctgccctg cgtcgggccc gagccgtcaa tcgccggtaa ggatgcggtc
      901 caccagctgc ttcacggttg gcgtgaagtt gttggaatag aacgggtcct gcttgaagcg
      961 gtaggccgcg tggcctgcga acatcaggtt cttgtcgatg tctccgccgt gggcgatgtc
     1021 ctgcagggtc ttctggatgc agaagctgcg gggatcggcg aggcgcccgg tggtgtaatc
     1081 atcgtggtcc ttccacgacg agaacccgca gtgcgacagg cagcccatgc agtccttctg
     1141 gtcctgctgg atttcgctgc gctcttcggg cgaaacgaac acgatcgtat cgtccggggt
     1201 gcgcaggcca tggacatagc cggcgccgaa ccaggagcgg gcgcgatcga gatcggcggg
     1261 ggcgacccag aagttcttgc ccttcacgcc aacatcgagc tgcaccgtgt gctcgcccgc
     1321 ggcaaccttg gaatagggga tttgccgctc agagcggccc tccagcgcgc gcaggaaggg
     1381 attgcgcacg gccgaggaat agaagccggt cggcgagaag cggtggagca ggacatcgcc
     1441 cggctcgagc gtgcgcaggt ggtccttcca gccctgcggg atcgggcttt ccgcggtgag
     1501 caggggccgg gtgccgaact ggaacgcgat ggcgccgagc tcgggattat cgatccagtc
     1561 attccattcg cgcagaaacc agacgccgcc agccatgacg atcggcacgc tatcggccac
     1621 gccttcggcg cgcatggtat cgcgcagggc cttgacgcgc ggatagggat cttcgggctt
     1681 gcgcggatct tccgcgttgg acaggccgtt gtgcccgccg gcaagccacg ggtcttcata
     1741 gacgaccgcg gccatgagat cggcaacctt gtgataggcg cgcttccaca gcgcccggaa
     1801 ggcgcggcca gagctgacga tcggcaggta gttgacgttg aagcgggcgg cgatctcgga
     1861 cagcttgtag ggcatgccgg cgccgcaggt cacgccggtg accaggccgc gggtcttttc
     1921 cagcacgcct tcgaggacct gctgggcccc gcccatttcc cacaggacgt tgatgttgat
     1981 cgcacccttg ccggaagcga ggtcgtaggc gcggcgcacc tgttcgacgg cgccatcgat
     2041 ggcatagcgg atcagctgtt cgtgccgttc cttgcgggtc agctgctcgt agacctgggg
     2101 gacgatcttg ccttcggcat cgtagctgtc ggcattgacc gcgctgaccg tgccgatgcc
     2161 gcccgccgcc gcccaggcac ccgagctcaa gtggttggtg gcggcgacgc ccttgccgcc
     2221 ttccaccaga ggccacacct cacggccgcc gtagaggatc ggttgaagtc ccttgaaagt
     2281 cattcgattc ctacttcccg ccgggttctt cccccgaaac gcctgccgca ttggcgacca
     2341 agcggccccc tgccgcatct ggggccaacc ggcaaggcct gatggcggcg ggcgcgggac
     2401 gcgggccggc gggttcgaac cgggcgtaat atccggatat tcccgggtta cgcgcaaatg
     2461 cggcgaggcg aaagcccagt gcgccgaatt cgcagaacaa caggtcgggt ggtgtgccgt
     2521 gctggtcggt agggcgatcg gcatcgacca cgacgaccga gccgcccgcc ttcagcgccg
     2581 gccgcatgtg ccacaaaaag gcatagggtt cagcaacttc atgatacatg tggaccatga
     2641 ggacgcgatc gaacgaggcc tgcggcagcc gcgggttatc gggcgtgccg agcttgatcg
     2701 agacgttgtc gagccgctcc ttctcgatcc tgcggccgag ccgctcgagc gcgccgggat
     2761 cgatgtcctc ggccagaacg cggccctttt tccccacttt ttccgcgagg cgcacggtat
     2821 aatagccctc gcccgcgccg atgtcggcca gggtcatccc cggctcgatg tgcgaccatt
     2881 ccattaccag gcgggcctcg cccagcctgt cgcgctggtc ttcggtggag aactgcgttg
     2941 aaatgatgcg tgaaaccggc cggtcggcgc gagggaagcc gcgggcttcg ggggaacggc
     3001 cggcgtcgcg atcaaccggc gcgctgcaac cgacctgcaa cgccgctgcc agcaccagca
     3061 gggccagtgc aagacggcgg cgcggccgca tcagtcgaca tcctcaacgg cgaccttctc
     3121 gccggtcacc cgctgggcga gagcggcggc catgaacggg tcgagcgcgc catcgagaac
     3181 gtcggacggg gtcggactgg tgaccccggt gcgcaggtcc ttgaccatct gataaggctg
     3241 gaggacgtag gagcggatct ggtggcccca gccgatgtcg ctcttgctgg catgttcggc
     3301 gctggccgct tcctcgcgcc gctgcatctc ggcctcgtag agccgggcct tgagcatgtt
     3361 catcgcgatt tcgcggttct tgtgctgcga gcggtcgatc tgcgaggcga cgatgatgcc
     3421 ggagggcacg tgagtgatgc gcacggccga gtcggtggtg ttcacgtgct ggccgccggc
     3481 gcccgaggag cgataggtgt cgacgcgcag gtccttgtcc tcgatctgca cctccacgtc
     3541 gcccgccacc tccggcacca cgtccaggga ggcgaaggag gtgtggcggc ggctctgggc
     3601 gtcgaaggga gagatgcgga ccaggcggtg cacgccgcgc tccgtgcgca ggtagccgta
     3661 ggcgttctcg ccgcggatcc cgagcgtgac gctcttgatc ccggcctcgt cgccggcctg
     3721 caggtcgagg g
//
LOCUS       DATNBV010000037         2049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_665019,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000037 DATNBV010000000
VERSION     DATNBV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665019"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VN627_00920"
     CDS             complement(<1..281)
                     /locus_tag="VN627_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HXD01845.1"
                     /translation="MSYVDGFVIAVPTANREAFIAHARQFDRLLVKEGALRVVECWGT
                     DVSRGKRTDFFGAVQAEEDETVCFSWVEWPDKATRDSCHARMQELMASG"
     gene            complement(320..757)
                     /locus_tag="VN627_00925"
     CDS             complement(320..757)
                     /locus_tag="VN627_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXD01846.1"
                     /translation="MARMIFVNLPVADLPRAKAFYAALGFVNEPRFTDETAAAMQWSE
                     TIVVMLLTHAKWATFTDRPIPSPSASEVMLCISCESREDVDRMTEAAAAHRGTADANP
                     AQDHGFMYSRSFTDPDGHLWEPMWMDAAIANGEAHVSEAHAAN"
     gene            824..1543
                     /locus_tag="VN627_00930"
     CDS             824..1543
                     /locus_tag="VN627_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXD01847.1"
                     /translation="MELQKETMGRSNKSPRRGRHGKWYDDACGTAFAMELIGERWALL
                     IVRELMFGGRRFSDIRASLPGLSAKVLTERLEALEQAGIVARVRLPPPASAQLYELTE
                     LGLAADEAIMALGRWAARSPAHDPTLPLSAASLGLSFRTMFDAGRAHGMDLTATLRIG
                     GEPFRVGISGGQLEIGRGEAEEPVFTLCAPSAMPIAAAVYGKVPLDELADAGLVLGGS
                     RAAALRFIDCFHLPGKLGPPH"
     gene            1645..>2049
                     /locus_tag="VN627_00935"
     CDS             1645..>2049
                     /locus_tag="VN627_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HXD01848.1"
                     /translation="MSDIPQIQPDTRDNTILAAPDTEVSVREVFGIDVDMTVPAFSVA
                     DERVPDRDESYVFDPDTTLAILAGFAYNRRVMVQGYHGTGKSTHIEQVAARLNWPCIR
                     INLDAHISRIDLIGRDAIVLRDGLQVTEFREGL"
BASE COUNT          335 a          689 c          645 g          380 t
ORIGIN      
        1 tcgccgctgg ccatgagctc ctgcatgcgg gcgtggcacg aatcgcgggt tgccttgtcc
       61 ggccattcga cccagctgaa gcagacggtc tcgtcctctt cggcctgaac ggcgccgaag
      121 aaatcggtcc gcttgccgcg gctcacatcg gtgccccagc attccacaac gcgcagcgca
      181 ccttccttca ccagcaggcg gtcgaactgc ctggcgtggg cgatgaaagc ctctcggttg
      241 gccgtgggca cagcgatcac gaaaccatct acatagctca tccgtggtct cctaatccct
      301 cccgccgatt gcttgtcgct cagttcgcgg cgtgtgcctc ggagacgtgt gcttcgccat
      361 tggcgattgc ggcatccatc cacatcggct cccacaggtg cccgtcgggg tcagtgaagc
      421 tgcgcgaata catgaagcca tggtcctgcg ccgggtttgc atcggctgtg ccacgatgcg
      481 ccgcggcggc ttcggtcatc cggtccacgt cctcgcggct ctcgcacgag atgcacagca
      541 tgacctcgct tgcagacggg gaagggattg gacggtcggt aaacgttgcc catttggcat
      601 gggtgagcag catcaccacg attgtctcgc tccactgcat ggccgcagcc gtttcgtcag
      661 tgaagcgcgg ctcgttcacg aatccgagcg cggcgtagaa tgccttggcg cggggaaggt
      721 cagccaccgg caagttgacg aagatcatcc gggccatcgg ccttctccca caatgcgaat
      781 cgatgttgcc acacgatgcg ccgttgagtt actcttagca actatggagt tacaaaaaga
      841 aaccatgggc cgctccaaca agtctccccg ccgtggccgt cacggcaaat ggtatgacga
      901 tgcctgcggc acggcgttcg cgatggagct gatcggcgaa cgttgggcgc tgctcatcgt
      961 gcgcgaactg atgttcggtg gaaggcgttt ttccgatatc cgcgccagtc tgcccggatt
     1021 aagcgccaag gtgctgactg agcggctcga agcgctcgag caggcgggca tcgttgcccg
     1081 cgtcaggctc ccgcctccgg cttcggcgca gttgtatgaa ctgaccgaac tggggcttgc
     1141 cgccgacgag gcgatcatgg cgctggggcg ctgggcggcg cgctccccgg cccacgatcc
     1201 caccctgccg ctctccgccg cctcgctcgg cctcagcttt cgcaccatgt tcgatgccgg
     1261 tcgggcccac ggcatggacc tgaccgctac cctgcggatc gggggggagc cgttccgcgt
     1321 cggcatttcc ggcgggcagt tggagatagg tcggggcgag gctgaggagc ccgtgttcac
     1381 tctctgcgca cccagcgcca tgcccattgc ggccgcggtc tatggcaagg tgccgctgga
     1441 cgagcttgcc gatgcgggac tggtgctcgg aggcagccgc gccgctgcct tgcggttcat
     1501 cgattgcttc cacctgccgg gaaagctcgg cccgccccat tgaggggcgc cgcagtgcat
     1561 caccctgttg cctcccgaaa gtgcgggcgc gatgcgaagg ttggatattg cagccgtggc
     1621 cgcgcgccgc tagtcgcact gggcatgagc gacattcccc agatccagcc cgacacgcgc
     1681 gacaacacga tcctcgccgc ccccgacacc gaggtgagcg tccgcgaggt gttcggcatc
     1741 gatgtcgaca tgacggtgcc ggccttctcc gtggcggacg aacgcgtgcc tgatcgcgac
     1801 gagagctacg tgttcgatcc ggacacgacc ctcgcgatcc tggcgggctt tgcctacaac
     1861 cgccgcgtga tggtccaggg ctatcacggc accggcaagt ccacccacat cgagcaggtc
     1921 gccgcccggc tcaattggcc ctgcatccgc atcaacctcg atgcccacat cagccgcatc
     1981 gaccttatcg gccgcgacgc catcgtcctg cgcgacggcc tgcaggtcac cgaattccgc
     2041 gagggcctg
//
LOCUS       DATNBV010000038         3098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1299440,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000038 DATNBV010000000
VERSION     DATNBV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299440"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VN627_00940"
     CDS             complement(<1..873)
                     /locus_tag="VN627_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HXD01849.1"
                     /translation="MSSAERAVRFYQAGGPEVLKLEDAAVGQPGPGEVRIRHAAVGCN
                     FADTYFRSGYYPVQPPCGIGVEGAGTIEAIGEGVTGFAVGDRVAYNGSPLGAYSTARV
                     MPAAPLFKLPESVSFEQAAASTMRGLSATYWLTRADPRLKAGDTILLHAAAGGVGLLA
                     VQLAKLLGLRVIGTVSTEEKAAMAKAHGCDEIIFYRREDVATRVKELTGGEGVQTAFD
                     SVGKDTFEGSLKSLRRRGVLVGCGTASGPLPPLDAFQMMLQGSVYFIRPAFADFYADP
                     TERAENAALWFGHLA"
     gene            1016..2017
                     /locus_tag="VN627_00945"
     CDS             1016..2017
                     /locus_tag="VN627_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXD01850.1"
                     /translation="MAEVVRVAAITGYQETMSGFGLDPRPFLREQGHNADMLANPERL
                     IPAVSAIRLLERSAEATRCETFGLRMAEGRSLANLGATSLLIAHQPTLRQALATIVEF
                     RTRINSTLVLHMEEVEGDIVLREDFQLSRPEPSRQASGLALGVLGRLCGTVLGDGWSP
                     RNLCFSHQPPPPDELPVFSRVFRCKPVFDCEFNGLVISPEDLDRPNQRADQQLALHAR
                     QLLELVMSPAERTARQDVDQLIKLLLPSAKASIQTCAGAMGVTVRTLQRSLEAEGESF
                     SNLLNQARMQLATQYLANPRMRVTDVADMLGYSSIGAFSRWHAQTFGKPPRRERRSR"
     gene            complement(2118..>3098)
                     /locus_tag="VN627_00950"
     CDS             complement(2118..>3098)
                     /locus_tag="VN627_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXD01851.1"
                     /translation="SGPSNLSGVLQASRSVAGVGLSGQIAYSIAPDARVESLAMALDK
                     ALGLHNRLSLGLTRSFRDRQTTVTAGWTRNFGSFGLGFSGLYGGPRRFGLGLQLFTAF
                     GPDPLRGRLVRDWQPLAQSGAVAARVFVDDNGNGRFDPGEQPVEGAGFAINGSGRQHV
                     RTNAEGEALLPRLSPRTWTSLALDPGTIEDARLQPEGAGVKVLPRPGKAQVVDLPVAA
                     VVEVDGTVWLATGVSRRPIGNTRLQLIGPDGRTVAETASASDGYFILPGVRPGQYSLR
                     VDPAQLAELHLMAIPTPVVVPAGGSFVNGLEITVKAAPAPPADAPVNVRR"
BASE COUNT          513 a         1050 c         1044 g          491 t
ORIGIN      
        1 ggccagatgg ccgaaccaca gggcggcgtt ctccgcccgc tcagtgggat cggcatagaa
       61 gtcggcaaag gccgggcgga tgaaatagac tgagccctgc agcatcatct ggaaggcatc
      121 gagcggcggg agcggccccg aagccgtgcc gcagccgacc agcacacccc ggcggcgcag
      181 cgacttgagc gatccttcga aggtgtcctt gcccacgctg tcgaatgccg tctgcacgcc
      241 ttcgccgccg gtaagttcct tgacgcgcgt ggccacgtcc tcgcggcggt agaagatgat
      301 ctcgtcgcaa ccgtgcgcct tggccatggc ggccttttcc tcggtggaga ccgtgccaat
      361 cacccgcagg cccagcagct tggccagctg gacggcgagc aggccgaccc cgccggcggc
      421 agcgtgcagc aggatcgtgt ccccggcctt gagccgcgga tcggcccggg tgagccagta
      481 ggtggccgag aggccccgca tcgtggaggc ggcggcctgt tcgaacgaga cgctctcggg
      541 caatttgaac agcggcgcgg ccggcatgac ccgggccgtg ctgtaggcgc ccagcgggct
      601 gccattgtag gccacacggt cgccaaccgc gaagccggta acgccctcgc cgatggcctc
      661 gatggtgccc gccccttcca cgccgatccc gcaagggggc tggaccggat agtagccgga
      721 gcggaaatag gtgtcggcga aattgcagcc cacggccgcg tggcggatcc gcacttcgcc
      781 gggacctggc tggcccacgg ccgcatcctc gagcttgagg acctccgggc cgccggcctg
      841 gtaaaatcgc acggcgcgtt ctgccgaact cattgctgca ctctccccgt gtgttgccaa
      901 gggggatagg ggccgcggcc cttgcctttt tcacatttca tgacattgtt ttgccattcg
      961 acgccacggc tgaggcaggg actggcgcgg cacatcgtcc gagggagaag gacgcatggc
     1021 ggaagtggtc agggtagccg cgatcaccgg ctaccaggaa acgatgagcg gctttggcct
     1081 ggacccgcgg cccttcctgc gcgagcaggg gcacaatgcg gatatgctcg ccaatcccga
     1141 gcggctgatc ccggccgtgt ccgccatccg cctgctcgaa cgcagcgccg aggcgaccag
     1201 atgcgagacc ttcggcctgc gcatggcgga gggtcgttcg ctcgccaatc tcggcgccac
     1261 cagcctgctg attgcccatc agccgacgct gcggcaggcc cttgccacga tcgtcgagtt
     1321 ccgtacgcgg atcaattcga ccctcgtgct gcacatggag gaagtcgagg gcgatatcgt
     1381 cctgcgcgaa gacttccagc tcagccggcc cgaacccagc cgccaggcct cgggcctggc
     1441 gctgggcgta ctggggcgac tgtgcggcac ggtgctcggc gatggctggt cgccccgcaa
     1501 cctgtgcttc tcgcaccagc ctccgccgcc cgatgaactg ccggtgtttt cccgcgtttt
     1561 ccgatgcaag ccggtctttg attgtgaatt caacgggctg gtcatctcac cggaggacct
     1621 cgaccgcccc aaccagcgcg ccgaccagca gctggcgctg catgcccggc aattgctcga
     1681 gctggtgatg agcccggccg agcgcaccgc caggcaggac gttgatcagc tgatcaagct
     1741 gctgttgccc tcggcgaagg cctcgatcca gacctgcgct ggcgccatgg gcgtgaccgt
     1801 tcgaaccctg cagcgaagtc tcgaggcgga gggcgagagc ttcagcaacc tgctcaacca
     1861 ggcgcggatg caactggcga cccagtacct tgcaaacccg cgcatgcggg tgaccgacgt
     1921 cgccgacatg ctgggctaca gttcgatcgg cgcattcagc cgctggcacg cgcagacctt
     1981 cggcaagccg ccccgacgag agcgacggtc caggtgatgg ctgctatggc ggagcgatga
     2041 tggcagatcg gcgcaactgc cggcttcccc catcagcttc gttgacgagc atggtcgcgg
     2101 ctgaactatt cccactatta ccggcgaaca ttgacaggcg catctgccgg cggggcgggc
     2161 gcagccttca ccgtgatttc gaggccgttg acgaagctcc cgccggctgg aacgaccacg
     2221 ggcgtgggta tggccatgag gtggagttcg gccagttggg ctggatcgac gcgcaggctg
     2281 tattgtcctg gccggacccc cggcaggata aagtagccat cgctggcgct ggcggtttcg
     2341 gcgacagtgc gcccgtcagg gccaatgagt tggagccggg tattgccgat cgggcggcgg
     2401 ctcacgccgg tagcgagcca gaccgtgccg tccacctcga ccaccgctgc aacggggaga
     2461 tcgaccacct gggcttttcc ggggcgcggg aggactttga ctcctgcccc ttccggttgc
     2521 aggcgcgcgt cctcgatcgt accggggtca agcgcgagac tggtccaggt gcgcggcgag
     2581 aggcgcggta gcagcgcctc gccctcagcg ttcgtgcgga catgttgccg gccgctgccg
     2641 ttgatcgcga agcctgcgcc ctcgaccggc tgctcgcccg gatcgaagcg gccattgccg
     2701 ttgtcgtcga caaatacccg cgcggcgacg gcgcccgact gcgcgagcgg ctgccagtcg
     2761 cgcaccaatc gcccgcgcaa gggatcgggg ccgaaggcag tgaacagctg gagcccgagg
     2821 ccgaaccgtc gcggaccgcc gtagagccca ctgaacccca ggccaaagct gccgaaattc
     2881 cgggtccacc cggcggtcac tgtcgtctgc cggtcgcgga acgaacgggt caggccaagg
     2941 ctcaggcggt tatgcaggcc cagcgccttg tcgagcgcca tggccaggct ctccacccgc
     3001 gcgtcggggg caatggaata ggcaatctgg cccgacaggc ccacgcccgc cacactccgg
     3061 ctcgcctgaa gcacgcccga cagattggac ggcccgct
//
LOCUS       DATNBV010000039         2263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_667239,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000039 DATNBV010000000
VERSION     DATNBV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667239"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..2125)
                     /locus_tag="VN627_00955"
     CDS             complement(80..2125)
                     /locus_tag="VN627_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXD01852.1"
                     /translation="MTIITEGTDLTIEDGIAVITLDYPPVNAMSPDLMDGLYDALMAA
                     LDDPAVKAIVLACAGRTFIAGADIKSMGSGKAPKVDFFELQDAVENSPKPTIAAMHGT
                     PLGGGLETAMTLHYRICTPKTRMGLPEVHLGLLPGGGGTQRLPRIVGAEAALDFLLTG
                     RQIPATDALAVGLVDAVSTSEDSLRADAMAFVRQVLADGRKPVRIRDREDKHLADQAD
                     AGLFDRLRQQWTANLKGMDAPQAIFRCVEASVKGPWDAGIKVERAEFWPLMNGAQSAA
                     LRHVFLAERAAAKIPGLSPEVKPRSIGKVAVIGGGTMGSGISAAFIDAGFPVTMVETS
                     QEALDRGAARVREVFASRAQRGKLSAEEAQARGALLSPTLDYAAIADADLVVEAVFES
                     MAVKKEVFAKLSAVARPDAILATNTSFLDVNEIATAATNPERVLGLHFFSPANVMRLL
                     EIVRAAKTSDEVLVTALDLAKKLKKVGVVSGVCHGFIGNRILRARAAQADALLGEGVS
                     PYDIDAALVEFGFPMGHFQMLDLAGLDIGEPDPARAALLKAGRKGQKSGAGFYDYAAD
                     RKGKPDQAALDLVGEAFGTKLGSKTVPQDELLARLLYPMTNEGAKILDEGIALRASDI
                     DMVYVTGYGWPAHKGGPMFYADLVGLPTVAAALKGMPGVTISPLLEKLAAEDGKFNG"
BASE COUNT          363 a          725 c          784 g          391 t
ORIGIN      
        1 cgctaagctt gccaaacgcg aaaaaaggcc ggcctcgcga tctgcggggc cggccttttc
       61 tgttgaggtt cgctccggtt cagccgttga acttgccgtc ttccgcggcg agcttttcga
      121 gcagcggcga gatggtcact ccaggcatac ccttaagagc ggcggcgacg gtcggcaggc
      181 cgaccagatc ggcatagaac atcgggccac ccttgtgcgc cggccagccg tagccggtga
      241 cgtagaccat atcgatgtcg gaggcgcgca gggcgatccc ttcatcgagg atcttcgcgc
      301 cttcgttggt catggggtag agcaggcggg ccagcagttc gtcctgtggc actgtcttcg
      361 agccgagctt cgtgccgaag gcctcgccga cgaggtcgag cgcggcctga tcgggcttgc
      421 ccttgcgatc tgcggcatag tcgtagaacc cggcgcccga cttctggccc ttgcggcccg
      481 ccttgagcag cgcggcacgg gccgggtcgg gctcaccgat atccagcccg gcgaggtcca
      541 gcatctggaa gtggcccatc ggaaagccga attccacgag cgcggcatcg atatcgtagg
      601 gcgagacgcc ttcccccagc agcgcatcgg cctgggccgc ccgggcgcgc aggatgcggt
      661 tgccgatgaa gccatggcac acgccggaga cgacgcccac cttcttgagc ttcttcgcca
      721 gatcgagcgc ggtgaccagc acctcgtccg aagtcttcgc ggcccgcacg atctcgagca
      781 gccgcattac gttcgccggc gagaagaagt gcagtccgag cacgcgctcc gggttggtgg
      841 ccgcagtggc gatctcgttg acgtcgagga agctggtgtt ggtggcgagg atcgcatccg
      901 gtcgggcgac cgcgctcaac ttggcgaaga cctccttctt tacggccatg ctctcgaaca
      961 cggcctcgac gacgaggtcg gcatcggcga tggccgcata gtcgagcgtg ggcgacagca
     1021 gggcgccgcg ggcctgggct tcttcggcgc tgagcttgcc gcgctgggcg cgcgaggcga
     1081 aaacctcgcg gacccgggcc gcaccgcgat caagcgcttc ctggctggtc tcgaccatgg
     1141 tcacgggaaa gcccgcatcg atgaaggccg cggagatgcc gctgcccatc gtgccgccgc
     1201 cgattacggc aaccttgccg atgctgcgcg gctttacctc gggtgagagg ccggggatct
     1261 tggccgccgc gcgctcggcc agaaagacat ggcgcagggc ggctgactgg gctccgttca
     1321 tgagcggcca gaattcggcg cgttcaacct tgatgccggc gtcccacggc ccttttaccg
     1381 aggcctcgac gcagcggaag atcgcttgcg gggcatccat ccccttgagg tttgccgtcc
     1441 attgctggcg cagccggtca aacaggccgg catcggcctg atcggcaagg tgcttgtcct
     1501 cgcggtcgcg gatgcgcacg ggcttgcggc cgtcggccag gacctggcgg acgaaggcca
     1561 tagcgtcagc gcgcaggctg tcctcggagg tcgacacggc atcgacgagc ccgaccgcga
     1621 gggcgtcggt ggccgggatc tggcggccgg tgagcaggaa gtcgagcgcg gcttcggccc
     1681 ccacgatccg gggcaagcgc tgggtgccgc cgccaccggg caggagcccg aggtgcacct
     1741 cgggcagacc catgcgggtc ttgggggtgc agatgcgata atgcagggtc atcgcggttt
     1801 cgagcccgcc cccgagcggg gtcccgtgca tcgccgcgat ggtcggcttg ggcgaattct
     1861 caacggcgtc ctgcagctcg aagaaatcga ccttgggcgc cttgcccgat cccatcgact
     1921 tgatatcggc cccggcaatg aaagtccgcc cggcgcaggc cagcacaatc gcctttacgg
     1981 ccggatcgtc cagcgcggcc atgagagcgt cgtagagccc gtccatcagg tctggcgaca
     2041 tggcgttgac cggcggatag tcgagcgtga tcacggcgat gccgtcttca atggtgagat
     2101 cggtaccttc ggtaatgatg gtcatggcat cctctggtga gtggtgcgga acagggttca
     2161 gcggcggctg gtaagtgcgc cgatattgac ccggtccctg cggaactcct ggttcgcatc
     2221 gcgcgagcga atgtgttcgt tgtcaaccgc cgcaccgtag gcg
//
LOCUS       DATNBV010000040         4735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1618826,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000040 DATNBV010000000
VERSION     DATNBV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618826"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..811)
                     /gene="urtD"
                     /locus_tag="VN627_00960"
     CDS             complement(71..811)
                     /gene="urtD"
                     /locus_tag="VN627_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HXD01853.1"
                     /translation="MTVPLLELENVTVDFDGFKAIDDLSLAIPPGSMTVVIGPNGAGK
                     STMCDAIIGRVRPTAGRVLLRGEEIQQAAEHEVVAKGICRKFQTPGVLSPLSVIDNLR
                     IAAATDRRWWKSLTRRPDRAAGQRAEEILEMVGLADRRHALAGNLSHGEKQWLEIGMV
                     VATDAELLLLDEPTAGMGAAESTRTAEIVKALVGKHSVLVIDHDIDFVSQLGGRVVVL
                     HQGQLLREGTLAEIRADAEVAAIYLGRA"
     gene            complement(808..1839)
                     /gene="urtC"
                     /locus_tag="VN627_00965"
     CDS             complement(808..1839)
                     /gene="urtC"
                     /locus_tag="VN627_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HXD01854.1"
                     /translation="MARALPLKPAAGAMAVLLALAAPWLLSAYDLNLLGRFLALSLTA
                     MGLVLLWGEGGVLSLGQGVFFGLGGYALAMHLKLADLGPGELPDFMVWTGVSALPWWW
                     ALFRSPWFAVLAVLAVAPALAALFAWAIFRRRIGGVYFALISQALALAFATLLISQQD
                     KTGGFNGLTDYHTLFGFNLNRPAAATVLYYITVLLTLLAFAGLSWLLAGRFGKLLRAS
                     RDGSNRLRFLGHDPVPYKIVAFTLAALLTSVSGALFTLHAGVVSPALVGVVPSIEMVI
                     WVAIGGRESVIGAILGTLLVNFAKDKISTALPELWLYGLGLLFIIAVTVLPKGIAGLF
                     ARRTGETGE"
     gene            complement(1854..2747)
                     /gene="urtB"
                     /locus_tag="VN627_00970"
     CDS             complement(1854..2747)
                     /gene="urtB"
                     /locus_tag="VN627_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HXD01855.1"
                     /translation="MDAAFLSGQLFNGFSVASLYILAALGLALSFGLMRVINMAHGEI
                     LMMGSYLAWLTMRFVPGPLGLVAAMAVAFVGAGLFGALLETTLISRLTARPLDTLLAT
                     WGVSLIMQQAARDLFGATGVSVTAPAWLDGGLTLGGLQLPTTRLFILLVTGLVLAGMA
                     ALLQFTRIGLLVRAVNQDRPTAAAMGVNIRLIDAVVFSLGAAVAGLGGVVLSLLGPVT
                     PNVGQSYIVTAFLVVILGGLGSIRGTAVAALMIGLFIALSQIFVDVSFAQVLSLVFVI
                     VFLQFRPQGVIAVRSRALDAE"
     gene            complement(2917..4122)
                     /gene="urtA"
                     /locus_tag="VN627_00975"
     CDS             complement(2917..4122)
                     /gene="urtA"
                     /locus_tag="VN627_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HXD01856.1"
                     /translation="MKKTLLAAMLAGTALISACKKDGAAAPAAGDAIKVGVLHSLSGT
                     MAISEVTVKNATQLAIDEINAAGGVMGKQVTAVVEDGASDPAIFAQKATKLVQDDGVS
                     TVFGGWTSASRKAMLPVFEKTGNLLWYPVQFEGNECSPNIMYSGAQPNQQALPAYDWA
                     KQKGYKKIFLVGSDYVYPRTANLILKTHIQKDGLTLSGEEYQPLGGTEFSGVIGKIKA
                     AKPDIIINTLNGDSNVAFFKQMAAAGMGPKVVPVMSFSIAEQEAMAMGPSLVDGGYTA
                     WNYYQSLTLPDNAKFIEAYKAKFGKDAVITDPMIHGYIDVYVWKAAVEKAQSTDPAKV
                     RAAAVTLDAIQTPMGPVKFMDNNSLVQTAYIGQSQPDGQFKIIWTSPAAVAPDPYDPV
                     TFPGKTCKI"
BASE COUNT          812 a         1537 c         1646 g          740 t
ORIGIN      
        1 acctggccct tgccataggc ggccgagacg ctgcgcaggt tcagcagctg accggggacc
       61 tcgatcgtca tcacgctctc cccaggtaga tcgcggcgac ttcggcgtcg gcgcggattt
      121 cggccagggt gccttcgcgc agcagctggc cctggtggag cacgaccacg cgcccgccga
      181 gctgggagac gaaatcgatg tcgtgatcga tgacgaggac cgaatgcttg cccaccagcg
      241 ccttgacgat ttcggcggtg cgggtgcttt cggccgcgcc catgccggcg gtcggctcgt
      301 ccagcagcag gagttcggcg tcggtcgcca cgaccatgcc gatttcgagc cactgcttct
      361 cgccgtggct gagattaccg gcaagcgcgt ggcggcgatc ggccaggccg accatctcga
      421 ggatctcctc ggcgcgctgg ccggcggcgc ggtcgggccg acgggtcagg ctcttccacc
      481 agcgccgatc ggtcgctgcc gcgatcctca ggttgtcgat caccgagagc gggctgagga
      541 cgcccggggt ctggaacttg cggcagatgc ccttggcgac gacctcgtgc tcggccgcct
      601 gctggatctc ctcaccgcgc aaaagcaccc ggccggcggt ggggcgcacg cggccgatga
      661 tcgcgtcgca catggtcgac ttgcccgcgc cgttaggtcc gatcacaacg gtcatcgagc
      721 cgggcgggat ggcgagcgag aggtcgtcga tcgccttgaa cccgtcgaaa tcgaccgtga
      781 cgttctcgag ttcgagcaag ggtacggtca ttcgccggtc tccccggtgc gcctggcgaa
      841 gaggccggcg atgcccttgg ggagcacggt cacggcaatg atgaacagca ggccgaggcc
      901 gtagagccac agttcgggca gggcggtgga gatcttgtcc ttggcgaagt tgaccagcag
      961 cgtgccgagg atagcgccga tcacgctttc gcgcccgccg atcgccaccc agatgaccat
     1021 ctcgatcgag ggcaccacgc cgaccagggc gggcgagacc acgccggcgt ggagcgtgaa
     1081 cagggcgccc gacacgcttg tgagcagcgc cgcgagggtg aaagcgacga tcttgtaggg
     1141 caccggatca tggccgagga agcgcagccg gttggagccg tcgcggctgg cccgcagcag
     1201 cttgccgaac cggccggcaa gcagccagct gaggcccgcg aaggcgagca gggtgagcag
     1261 caccgtgatg taatagagca cggtggccgc ggccggacgg ttgaggttga agccgaacag
     1321 ggtgtgatag tcggtcaggc cgttgaagcc gccggtcttg tcctgctggc tgatcagcag
     1381 ggtggcgaaa gccagcgcca gggcctgcga gatcagcgcg aagtagaccc cgccgatccg
     1441 ccggcggaag atcgcccagg caaacagcgc ggcgagcgcg ggcgccaccg ccagcacggc
     1501 gagcaccgcg aaccacgggc tgcggaacag ggcccaccac cagggcagcg cgctgacccc
     1561 ggtccagacc atgaagtccg gcagttcacc ggggccaagg tcggccagct tcaggtgcat
     1621 ggcaagcgca tagccgccga gcccgaagaa cacgccctgc cccaggctca gcaccccgcc
     1681 ctccccccac agcaggacta ggcccatggc ggtgagcgac aaggcgagga agcggccgag
     1741 caggttgagg tcgtaggcgc tgagcagcca cggcgcggcc agggcaagga gcacggccat
     1801 ggcgccggcg gcgggcttga gcggcagggc gcgggccatc ggacgcaaag ctcctattcc
     1861 gcatccagcg cgcgggagcg aacggcgatc actccctgcg gccggaactg gaggaagacg
     1921 atcacgaaga cgaggctcag cacctgggcg aagctcacgt cgacgaagat ctgcgaaagg
     1981 gcgatgaaca ggccgatcat cagcgctgcc acggccgtcc cgcggatcga gccgaggccg
     2041 ccgaggatga cgacaaggaa ggccgtgacg atatagctct ggcccacgtt cggcgtcacc
     2101 ggcccgagca gggagaggac caccccgccc agccccgcca cggccgcgcc cagcgagaac
     2161 acgacggcat cgatcaggcg gatattgacg cccatcgcgg cggcggtggg ccgatcctgg
     2221 ttcaccgcgc gcaccagcag gccgatccgc gtgaactgga gcagggccgc catcccggcg
     2281 agcaccagcc ccgtcaccag caggatgaac aggcgggtgg tgggcaactg caacccgccg
     2341 agcgtcagcc cgccatcaag ccatgccggc gcggttaccg aaacgccggt cgcgccgaac
     2401 aggtcgcgcg cggcctgctg catgatcagg ctcacgcccc aggtcgccag cagggtatcg
     2461 agcgggcgcg cggtcagccg gctgatcagg gtggtctcaa gcagggcgcc gaacaggccg
     2521 gcgccaacga aggccacggc catggccgcg accagcccca gcgggcccgg cacgaagcgc
     2581 atggtcagcc aggcgaggta gctgcccatc atcaggattt cgccatgcgc catgttgatc
     2641 acgcgcatca gcccgaagct gagcgcgagc ccgagcgcgg ccaggatata gagcgaggcg
     2701 acgctgaagc cgttgaacag ctgaccagac aggaaggcgg cgtccattgc gactgtcctc
     2761 ggtttcgtca acttggcgga ggctgcggcc aactcctgcc tcgcagcgag gctccagttt
     2821 cgctggcgag gcaggaggcg gcggtccacc gctggggggg cgacggggct ggcctgaggg
     2881 gtcccggccc gccgcccaag tggtcagacc agttgatcag atcttgcagg tcttgcccgg
     2941 gaaggtgacc ggatcgtagg gatcgggcgc caccgcggcc ggcgaggtcc agatgatctt
     3001 gaactgcccg tcgggttgcg actggccgat gtaggcggtc tggaccaggc tgttgttgtc
     3061 catgaacttg accgggccca tcggggtctg gatggcgtcg agcgtgaccg ccgcggcgcg
     3121 gaccttggcc ggatcggtgc tctgcgcctt ctcgaccgcg gccttccaga catagacgtc
     3181 gatgtagccg tgtatcatcg ggtcggtgat caccgcgtcc ttgccgaact tggccttgta
     3241 ggcctcgatg aacttggcgt tatccggcag cgtcaggctc tggtagtagt tccaggcggt
     3301 atagccgccg tcgaccagcg acgggcccat cgccatggct tcctgctcgg cgatcgagaa
     3361 tgacatcacc ggcacgacct tgggccccat gcccgcggcg gccatctgct tgaagaacgc
     3421 aacgttgctg tcgccgttca gggtgttgat gatgatgtcg ggcttggccg ccttgatctt
     3481 gccgatcacg ccggagaatt cggtgccgcc aagcggctgg tattcttcgc ccgaaagggt
     3541 cagcccgtcc ttctggatgt gggtcttgag gatcaggttg gccgtgcgcg gataaacgta
     3601 gtccgagccg accaggaaga tcttcttgta gcccttttgc ttggcccagt cataggcggg
     3661 gagggcctgc tggttgggct gcgcgcccga atacatgatg ttgggcgagc attcgttgcc
     3721 ctcgaactgg accggatacc acaggaggtt gccggtcttc tcgaacaccg gcagcatcgc
     3781 cttgcggctg gccgaggtcc acccgccgaa gacggtggag acgccgtcgt cctggaccag
     3841 cttggtcgcc ttctgggcaa agatcgccgg gtcggaagcg ccatcctcga ccacggctgt
     3901 tacctgcttg cccatgacgc cgccggcggc gttgatctcg tcgatcgcga gctgggtcgc
     3961 gttcttcacg gtgacttcgc tgatcgccat cgtgccggac agcgaatgca gcacgccgac
     4021 cttgatcgca tcgccggctg ccggggcggc ggccccatcc ttcttgcacg cggaaatcag
     4081 agcggtcccc gcgagcatcg cagccagcaa ggtctttttc atcgcggtca tggcatgtca
     4141 tccttgtatg gcggcttgcc gctgaggcag gcccgcctgg tctgcagggt caggggatca
     4201 gaagcggcgt tccggcgacg ggcgtcccga ccgatacgat gcgtgtggcc ggccgcggtc
     4261 ggcctcgccc agggccagcc gccccggcgc ggcgtgcgct cgtttcatca tgagttaccc
     4321 ccttgcgccg cccggcgcct cgcttgccac catcgcggcg gccccctgtc atggtgcacc
     4381 gcaacagaca atggccatgg ggtatttgac gtaggtcgga tgaccagggc aggagggcgg
     4441 cggggattga ggaaggaccg attgtccatg gggtaaatgc cccatccggc ccggccagac
     4501 cggctgttac tcacccttca tgagaacgac ttccagtctg gcgattgcgg aaccggggcc
     4561 tgccgcgccg gccagccccg caggcggcga taatcggttg tcatcgccgg tagaggtgga
     4621 ttacgctgca ctgcactata ggattcgcag tcggggagcg ctcgatatcg tattccgcgc
     4681 ggagaaccac cgttcgcctc tggcccgcag ctttcaatct ggctgcctga ggttg
//
LOCUS       DATNBV010000041         3156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3853438,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000041 DATNBV010000000
VERSION     DATNBV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3853438"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VN627_00980"
     CDS             <1..250
                     /locus_tag="VN627_00980"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXD01857.1"
                     /translation="YNPALFWDAKAGSEKATLPPGPNGPVGVVWIDLTKPHYGIHGTP
                     NPETIGRAESHGCVRLTNWDAARLAMMVHPGTKVSFVP"
     gene            247..951
                     /locus_tag="VN627_00985"
     CDS             247..951
                     /locus_tag="VN627_00985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXD01858.1"
                     /translation="MSTRMGGYGRLRLIATTVVVTSLAWIAGLAVWFQARPAAAPPVP
                     VQSAALPAVLPARATSALVIPVLGVPRSQLVDTFTQSRANGARRHDAIDIMAPRGTPV
                     IAAAPGRVEKLFLSHDGGNTIYVRSPDGRLIYYYAHLDAYAPGLTMGQEVTAGTPLGT
                     VGSTGNADPAGPHLHFAIAEADPTAPWYQQAPALNPYPWLVSGQRPELGARTQRSAPS
                     LPSADSRRRNDLPERE"
     gene            complement(855..1235)
                     /locus_tag="VN627_00990"
     CDS             complement(855..1235)
                     /locus_tag="VN627_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXD01859.1"
                     /translation="MPVCRLAACSLALACTVLAVGKAGAAPGGPLGTLPLGVYNCELP
                     GDAEGAAGVPQPAEGFTIIHGSGYSTATGRGVYLMTGERVEFTSGPLRGVAYSRSGRS
                     FLRRLSADGSEGALRCVRAPNSGR"
     gene            1367..1933
                     /gene="efp"
                     /locus_tag="VN627_00995"
     CDS             1367..1933
                     /gene="efp"
                     /locus_tag="VN627_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXD01860.1"
                     /translation="MAKISGVDIRPGNILEYEKGIWKVAKTQHTQPGKGGAFMQVEMK
                     NLIDGRKTNVRFRSADTVERVRLDTKDFQFLYAEGDDLVFMDTETYDQITLPKDLLGG
                     ADAFLQDGMTVLLEMYDERPISVQLPEQVEATIVEADAVVKGQTASSSYKPAILDNGV
                     RVMVPPHIESGTRIVVDVYERTYVGKAN"
     gene            1938..2756
                     /locus_tag="VN627_01000"
     CDS             1938..2756
                     /locus_tag="VN627_01000"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXD01861.1"
                     /translation="MAVISGLIRVMEKAARKAGQRLRRDFGEIEHLQVSRKGPADFVS
                     KADQAAERTLWDELRAARPDWGFELEEGGTIAGAEGKPRFIIDPLDGTSNFLHGIPHF
                     AISIAVQEPRLDGKGWGEVTAALVYQPITDESFWAEKSKGAWLQDKRLRVSGRRHLDE
                     SLIATGMPFAGHGDFAEWVKIFAAIGPQVAGIRRFGSAALDLAWVAAGRYDGFWESGL
                     KPWDTAAGCLLVREAGGFVTNFKGISEPVCAAQVLAGNDALHSRLHKIVAAALK"
     gene            2820..2903
                     /locus_tag="VN627_01005"
     tRNA            2820..2903
                     /locus_tag="VN627_01005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2853..2855,aa:Leu,seq:caa)
     gene            2972..>3156
                     /locus_tag="VN627_01010"
     CDS             2972..>3156
                     /locus_tag="VN627_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01862.1"
                     /translation="MADEVYTRDGAPRETTIIHETRSSGGAGWVIAVVLIIALIAGIW
                     FFTQSNRSQVARDNAVTA"
BASE COUNT          542 a         1059 c         1057 g          498 t
ORIGIN      
        1 ctacaacccg gcgctgttct gggatgccaa ggcggggagc gagaaggcca cgctgccgcc
       61 ggggcccaac gggccggtcg gcgtcgtgtg gatcgacctg accaagccgc actacggcat
      121 tcacggcacc cccaatcctg aaaccatcgg acgggcggaa agccatggtt gcgttcggct
      181 gaccaactgg gatgccgcgc gacttgccat gatggtgcat ccgggcacca aggtttcgtt
      241 cgttccatga gcacgcggat gggcggttat ggacggctgc ggctgatcgc cacgaccgtg
      301 gtcgtcactt cgctggcgtg gatcgccggg ctggcggtct ggttccaggc ccgcccggct
      361 gccgcgccgc cggttccggt gcaatctgcg gccttgccgg cggtcttgcc agcgcgggcc
      421 acatcggcgc tggtcattcc cgtgctcggc gtcccccggt cacagctggt ggataccttc
      481 acccagtccc gcgcgaacgg ggcccggcgc catgatgcga tcgacatcat ggcgccgcgc
      541 ggaacaccgg tcatcgcggc cgcgccgggg cgggtggaga agctgttcct ctcgcacgac
      601 ggcggcaaca cgatctacgt ccgatcgccg gacgggcggc tgatctatta ctacgcccac
      661 ctggatgcct atgcaccggg attgaccatg gggcaggagg tgacggcagg cacgccgctg
      721 gggacggtcg ggagcaccgg caatgccgat ccggccgggc cccacctgca cttcgccatt
      781 gccgaggctg atccgaccgc gccgtggtac cagcaggcgc cggcacttaa cccctatccg
      841 tggctggtct ccggtcagcg gccggaattg ggcgcccgca cgcagcgcag cgcgccttcg
      901 ctgccatcgg ccgacagccg gcgcaggaac gatctgcccg agcgcgaata ggcgaccccg
      961 cgcaggggac ccgacgtgaa ctccacccgc tcgccggtca tcaggtaaac gccgcgtccg
     1021 gttgccgttg aatagcccga gccatggatg atagtgaagc cctccgccgg ttgcggcacg
     1081 cctgccgccc cttcggcgtc gcccggcagc tcgcagttgt agacgcccag cggcagggtg
     1141 ccgagcggcc cgccgggcgc ggcgccagcc ttgcctacgg caaggacagt gcaggcaagg
     1201 gcaaggctac aggcggcgag gcggcaaacg ggcatggctg cctgcctagc actccgctgc
     1261 ttccaacgcc actgcttgcc ccggcccgcg caccgcgcta agggcgcgcg ttccggccgg
     1321 tggcatgcca gcggccaagc tctttcccaa cctgaaggcg tggcgcatgg cgaagatcag
     1381 cggcgtggac atccgtcccg gcaatatcct ggaatatgaa aagggcatct ggaaggtcgc
     1441 caagacccag catacccagc cgggcaaggg cggcgccttc atgcaggtcg agatgaagaa
     1501 cctcatcgac ggccgcaaga ccaacgtccg cttccgctcc gccgacacgg tggagcgcgt
     1561 gcgcctcgac accaaggact tccagttcct ctatgccgag ggcgacgatc tggtgttcat
     1621 ggacaccgaa acctacgacc agatcaccct gcccaaggac ctcctcggcg gcgccgatgc
     1681 cttccttcag gacggcatga ccgtgctcct cgaaatgtac gacgagcgcc cgatctcggt
     1741 gcagctgccc gagcaggtgg aagccaccat tgtcgaggct gacgccgtgg tgaaggggca
     1801 gaccgcctcc tcgtcgtaca agccggcgat cctcgacaac ggcgtgcgcg tcatggtccc
     1861 gccgcacatc gagagcggaa cccgcatcgt ggtcgacgtt tacgagcgga cctacgtcgg
     1921 caaggcgaac tgatcacatg gcagtcattt ccggcctgat ccgcgtgatg gagaaggctg
     1981 cccgcaaggc cgggcagcgc ctccgccgcg atttcggcga gatcgagcat cttcaggtca
     2041 gccgcaaggg cccggccgat ttcgtctcca aggcggatca ggccgccgag cgcaccctgt
     2101 gggacgaact gcgcgcggcg cggccggact ggggcttcga gctcgaggaa ggcggaacga
     2161 ttgccggggc cgaaggcaag ccgcgcttca tcatcgatcc gctcgacggc accagcaact
     2221 tcctccacgg cattccgcat ttcgcgatct cgatcgcggt gcaggagccg cggcttgacg
     2281 ggaagggctg gggagaagtg accgcggcgc tggtctacca gccgatcacg gacgagagct
     2341 tctgggccga aaagtccaag ggcgcctggc tgcaggacaa gcgcctgcgt gtctccggcc
     2401 gccgccatct cgacgaatcg ctgatcgcca ccggcatgcc ctttgccggg catggcgatt
     2461 ttgccgaatg ggtgaagatc ttcgccgcaa tcggcccgca ggtggccggc atccgccggt
     2521 tcggctcggc cgcgctcgat ctcgcctggg tggctgccgg ccgctacgac gggttctggg
     2581 aaagcggact caagccgtgg gacacggcgg caggctgcct gctggtccgc gaggccggtg
     2641 gttttgtgac caacttcaag ggcatttccg aaccggtctg cgccgcccag gtgctggccg
     2701 gcaacgatgc cctgcattcc cggctgcaca agatcgtcgc cgcggcgttg aagtaagatt
     2761 gtgaacggca gcgcgggacg ctaaggctcg cgcggccgca cccggttctg cggaattggg
     2821 cccctgtggt ggaatggtag acgcgaccga ctcaaaatcg gttggagaaa tccgtgccag
     2881 ttcgagtctg gccaggggca ccatcggctt cggaactcat ggcagttttc gggcatttcg
     2941 tcggcaaacc caccgatgaa ggagccatgc catggccgac gaagtctaca cccgggacgg
     3001 tgcgccgcgc gaaacgacca tcatccacga aacccgctcg agcgggggtg ccggctgggt
     3061 gattgcggtg gtgctgatca ttgccctcat cgccgggatc tggttcttca cccaaagcaa
     3121 ccgcagccag gtcgcgcggg acaatgccgt gaccgc
//
LOCUS       DATNBV010000042         7910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4179195,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000042 DATNBV010000000
VERSION     DATNBV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4179195"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VN627_01015"
     CDS             complement(<1..706)
                     /locus_tag="VN627_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXD01863.1"
                     /translation="MNFRNISAWSIRNPVVPIVLFAGLVLAGIVSFLHMEVQQQPDIE
                     FPVVIVTVTQPGAAPSEIETQITQRVESAIRSIAGVNSISSTATEGSSQTIIEFQLGE
                     NINEAVNEVKNAVDQIRSDLPEGILEPSVIKAQTSSEPIGYFAVTAQDMSIEQLSWFV
                     DDTVAKRLLAIEGMATVTRGGGVNREILVTLDPPRMQAAGVTAAQVNTALRQLNTNAA
                     GGRAEIAGSRQSVRVLG"
     gene            complement(717..1901)
                     /locus_tag="VN627_01020"
     CDS             complement(717..1901)
                     /locus_tag="VN627_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXD01864.1"
                     /translation="MNFEARIASEEGERAPELAQGPVASRRTWLVIAGAAAMLVAIWF
                     LIHRGDRPAAADSASQAATVSVVAPGRATVVGAINATGTIAARYDEPVGSVGEGGQVV
                     EVRAEAGQWVRKGQVLAVIDRSVQAQAQASQAAQIRVADADARLAQANLDRGLKLVDR
                     GFISRADIDRLTATRDAAVARVGVARASLGQLQAQSARLTITAPSDGLVLERKVEKGQ
                     VVGAASGTLFRIARGGDLEVLARLSETDLAAIATGVSAEVTPVGSPRSFAGQVWQISP
                     VIDPQTRQGTARIALAYAPELRPGGFASVVIRAGSVVAPLLPESAILSDDKGSFVYIV
                     GAGNKVERRPVRTGTVSDAGIAVIEGLTGAERVVLRAGGFLQPGETVNPKVVSASAAA
                     AR"
     gene            complement(2095..2394)
                     /locus_tag="VN627_01025"
     CDS             complement(2095..2394)
                     /locus_tag="VN627_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HXD01865.1"
                     /translation="MIENQNIRPAMVIGPLGEPLTIDSLPPPSTTRWVVRRKAEVVAA
                     VNGGLLTIEEACERYNLTLEEFASWQRAVDRSGMQGLRVTRIQHYRDLYERQLKY"
     gene            complement(2387..2770)
                     /locus_tag="VN627_01030"
     CDS             complement(2387..2770)
                     /locus_tag="VN627_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01866.1"
                     /translation="MGFDFHDGAQGPGSPAQTGGFAVPWDLRGQPLPGALAPDGPLWS
                     LDWMSLKARFAAIHALRRTIDSSQEQPVPAGGFMAAGEAVLASARGNVEAVNRLCSGN
                     GKSGAGMIALLSPTVPREATEDTND"
     gene            2906..4084
                     /gene="mnmA"
                     /locus_tag="VN627_01035"
     CDS             2906..4084
                     /gene="mnmA"
                     /locus_tag="VN627_01035"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXD01867.1"
                     /translation="MEAPEISLNEVDLASLFQLSPGPDGTLAGRRIVVAMSGGVDSSV
                     VAALAARSGAETIGITLQLYDYGAATGRKGACCAGDDIRDARAVADRLGIAHYVFDHE
                     SSFREEVVERFADDYLAGRTPIPCIRCNMGPKFTDLLQMARELGADCLATGHYVKRVA
                     GSLARLGAGPELHRAADPARDQSYFLYATTEAQLDFLRFPLGSLPKSAVRELAAEAGL
                     RVAAKPDSQDICFVPDGDYAKVVKAVRPEGVAGGDIVHAESGAVLGQHAGVIHYTVGQ
                     RRGLEIGGQPEPLYVTGIDAAARRVIVGPRRLLAVSAATLTDTNRIGPLPDGPLTAKV
                     RSLARPVPVTLDGPLGEGATTRIRFAAPEFGVAPGQAAVIYAGERVVGGGWIEGTEAA
                     "
     gene            complement(4086..4457)
                     /locus_tag="VN627_01040"
     CDS             complement(4086..4457)
                     /locus_tag="VN627_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01868.1"
                     /translation="MATSRPASSTAHSRALPRRPLFRRLLWVVVIALAAAVLLYGPTL
                     AALARTGSAYGARIGCSCRFVEGRPLGQCRKDFEGGMGLVTLSEDETARSVTARFALA
                     FPQTATFTEGAGCQLQPWPRD"
     gene            4490..5620
                     /locus_tag="VN627_01045"
     CDS             4490..5620
                     /locus_tag="VN627_01045"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXD01869.1"
                     /translation="MSTRSRTFIRNLALASLALVAGCGQQAAMQAPLDPAALAVVKPA
                     AGVPREKLARAIDGLFADPAAGETRALLVLVDGKMVAERYGPGFDAGTRQLGWSLSKT
                     LTGLMIGLLVSDGRLRLDESVPVPAWQRSGDPRGEITLRQLLQMRSGLRHVEEASPVY
                     TSDTARLLLGDGHDDTAAYAEAQPLAAEPGRKFVYSSATGAILSDLAARVLADGADPA
                     LRRQVVGEYLSTRLLVPLGMASAVAEYDRAGTLMGGGMIHATARDWSKLGEFLRNGGA
                     VHGAQILPRSWVEFMTQPSPAEPGYGAQLWLNRDRADGADRLLPGKLPAGIFAAVGHL
                     GQYVIVSPAQKVTLVRLGHSDNAERQAVRERLAEILRVLPKG"
     gene            complement(5621..6550)
                     /locus_tag="VN627_01050"
     CDS             complement(5621..6550)
                     /locus_tag="VN627_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXD01870.1"
                     /translation="MTDPQTAGLNRSAALASIGVATLLLGMKAFAAWQTGSTAMLGSL
                     ADTALDLVASLATLAGVWVAAQPDDLHHRFGHGKAEALAAMFQVVLISISALGLAFRA
                     VEQFFAGGRTAAAGEGIVVSAVAMAATLGLLAWQRHVIRKTNSLAISTDHVHYQSDLL
                     LNLAVIAALALDQYFGIAGADPLFGLVIAAWLGWGAWGASTAAIEQLMDREWPQEKKK
                     RFLEVIARHPELRGVHDLRTRTSGNRDFVQFHVWVDRNMTIAAAHDVMDAIEAKIQGE
                     FPGVEILIHPDPEGHVDAGLAGRPDLTAAQIKA"
     gene            complement(6561..7187)
                     /gene="pdxH"
                     /locus_tag="VN627_01055"
                     /pseudo
     CDS             complement(6561..7187)
                     /gene="pdxH"
                     /locus_tag="VN627_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
     gene            7363..>7910
                     /locus_tag="VN627_01060"
     CDS             7363..>7910
                     /locus_tag="VN627_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXD01871.1"
                     /translation="MADPYATLGVARGASEKDIKSAYRKLAKELHPDRNKDNPKAAER
                     FSEVTGAYDLLSDKDKRARFDRGEIDADGNPTSPFGGGGFGGGGQRGFRHDGFEGFGG
                     QQEGVDISDLFEGLFGGRAAGGGGGGPFGGAGGGGFGGMGGGRRAPAKGANVQYRLKV
                     SLTDAAILAAQRITLSDGKTIDL"
BASE COUNT         1252 a         2562 c         2908 g         1188 t
ORIGIN      
        1 tgccgaggac acgaaccgac tggcgcgagc cggcgatctc cgcccggcct ccggcggcat
       61 tggtgttgag ctggcgcagg gccgtgttga cctgggccgc cgtgacgccg gccgcctgca
      121 tgcgcggggg atcgagcgtg accaggatct cgcggttgac cccgcccccg cgggtgaccg
      181 tggccatgcc ctcgatcgcg agcaggcgtt tggccacggt atcatcgacg aaccacgaga
      241 gctgctcgat ggacatgtcc tgcgcggtaa ccgcgaaata gccgatcggt tcggacgagg
      301 tctgcgcctt gatgaccgaa ggttcgagga tgccttcggg caaatcgctg cggatctggt
      361 cgaccgcgtt cttcacctcg ttgacggctt cgttgatatt ctcgcccagc tggaactcga
      421 taatcgtctg gctcgagccc tcggtcgcgg tcgaactgat cgaattgacg cccgcgatcg
      481 agcggatggc ggattccacc cgctgggtga tctgggtctc gatctcgctc ggcgcggccc
      541 ctggctgggt gaccgtgacg atgaccacgg ggaattcgat gtcgggctgc tgctgcactt
      601 ccatgtgcag gaagctgacg atccccgcca ggaccagacc ggcaaacagg acgatcggca
      661 ccaccgggtt gcggatcgac caggcggaga tgttgcggaa gttcacgagg acgggctcag
      721 cgcgccgcgg cggcggaagc ggaaacgacc ttgggattca ccgtctcgcc cggctggagg
      781 aagccgccgg cgcgcagcac tacgcgttcc gccccggtga gcccttcgat cacggcgatg
      841 ccggcatcgg agacagtgcc ggtgcgcacg gggcgccgtt ccaccttgtt gccggcgcca
      901 acgatgtaga cgaagctgcc cttgtcatcc gagaggatcg ccgattccgg gagcagcggc
      961 gcgaccacgc tgccggcgcg gatcaccacg ctggcaaacc cgcccggccg cagttcgggc
     1021 gcataggcga gggcgatgcg ggcggtaccc tggcgggtct gcggatcgat caccggggaa
     1081 atctgccaga cctggccggc gaaagagcgc ggcgaaccga ccggggtcac ttcggccgag
     1141 acgccggtgg cgatggcggc gagatcggtt tccgaaagac gggccagcac ttcgaggtcc
     1201 ccgccgcggg cgatgcggaa gagcgtgccg ctcgcggcgc cgacgacctg gcccttctcg
     1261 accttgcgtt cgagcaccag accgtcagac ggggcggtga tggtcagccg cgcggactgg
     1321 gcctggagct ggccaaggct ggcccgggcg acgccgaccc gggccacggc ggcatcgcgg
     1381 gtggcggtga gccgatcgat gtcggcgcgg gagatgaagc cgcgatcgac cagcttgagg
     1441 ccgcgatcga gattggcctg agccaggcgg gcatcggcat cggcgacgcg aatctgggcg
     1501 gcctggctgg cctgggcctg ggcctggact gaacggtcaa tcacggcgag gacctggccc
     1561 ttgcggaccc actggccggc ttcggcacgg acttcgacta cctgcccgcc ttcgccgacc
     1621 gagcccaccg gctcgtcata gcgggcggca atggtgccgg tggcgttgat tgcgccgacc
     1681 acggtggcgc ggccgggggc tacgacgctg acagtggcgg cctggctggc gctatcggcc
     1741 gcggccgggc gatcgccccg gtgaatgagg aaccagatgg caaccagcat cgcggcggca
     1801 ccggcgatga ccagccaggt ccggcggctg gcgacggggc cttgggcgag ttcgggcgcc
     1861 ctttcgcctt cctcgcttgc gatcctggct tcgaagttca ttggagaagg ccgccttatc
     1921 cgcgcggaag cgcacgggaa atcgtattta aggtcaggat cgccgcggac aagccggggc
     1981 caacctaggg tcaggaccct aacgacagcg gcataaagac gcaaacgccc gggcgcaatc
     2041 gatgcggccc gggcgagaca atttgttact cccgggccta gcccgacagc gcgatcaata
     2101 cttgagctgg cgctcgtaga gatcgcggta atgctggatg cgggtgacgc gcaggccctg
     2161 catgccggag cgatcgaccg cgcgttgcca cgaggcgaat tcctcaagcg tgaggttata
     2221 gcgctcgcag gcttcctcga tggtcaacag cccgccgttg acggcggcga ccacttcggc
     2281 cttgcgccgc acgacccagc gggtggtgct cggcgggggc agggaatcaa tcgtcaacgg
     2341 ctcgcctagc ggaccgatca ccattgcggg gcggatgttc tggttctcaa tcattggtat
     2401 cctcggtcgc ctcgcgtggg actgttggcg acagcagcgc gatcatgccc gcccctgatt
     2461 tgccattgcc cgaacaaaga cggttaacgg cttcgacgtt cccgcgtgcc gacgccagca
     2521 cggcttcgcc cgccgccatg aacccacccg ccgggacggg ctgctcctgc gacgaatcga
     2581 tggtccgccg cagcgcatgg atggccgcga agcgggcctt gaggctcatc cagtcgagcg
     2641 accagagagg cccgtcgggc gccagggcac cgggcagagg ctgcccgcgc aagtcccacg
     2701 gcaccgcgaa accgccggtt tgcgccggcg agcccgggcc ctgcgcccca tcgtggaagt
     2761 cgaatcccat ctgcatggcg acggtcttac gcccgggttg gtcaagggac ggtaaacgcg
     2821 ggttttaccg ctcggagaga ttggttaacg tattgcaggg gcaccgattt tggcgattca
     2881 gccgaccgcg tgtatggggc ggctcatgga agcgccggaa atcagtctta atgaggtgga
     2941 cctggcaagc ctgttccagc tgtcgccggg accggacggt acgctggccg ggcggcggat
     3001 cgtggtggcg atgtcgggcg gggtcgacag ctcggtggtg gcggccctcg ccgcccgcag
     3061 cggcgccgag acgatcggca tcacgctcca gctctatgac tatggggcag ccaccgggcg
     3121 caagggcgcc tgctgcgcgg gcgacgacat ccgcgacgcg agggccgtgg ccgaccgcct
     3181 cgggattgcc cattatgtat tcgaccatga gagcagtttc cgcgaggagg tggtcgagcg
     3241 gttcgccgac gattacctcg ccgggcgcac gccgatcccc tgcatccgct gcaacatggg
     3301 gcccaagttc accgacctcc tgcagatggc ccgcgagctg ggcgccgatt gcctggcgac
     3361 cggtcactat gtgaagcgcg tggcggggag tctggcacgt ttgggcgccg ggccggaact
     3421 gcaccgtgcc gccgatccgg cgcgcgacca atcctacttc ctctacgcca cgaccgaggc
     3481 acagctcgac ttcctgcgct ttccgctggg cagcctgccc aaaagcgcgg tgcgcgagct
     3541 ggccgcggaa gcgggcctca gggtcgcggc caagcccgac agccaggaca tctgcttcgt
     3601 gcccgacggc gactatgcca aggtggtcaa ggcggtgcgg cccgagggcg tggccggggg
     3661 cgacatcgtc catgccgaat cgggcgccgt ccttggccag catgccgggg tgatccacta
     3721 taccgtaggc cagcggcgcg ggctggagat cggcggccag cccgagccgc tctacgtgac
     3781 cgggattgat gccgccgcgc ggcgggtgat cgtggggccg cggcgcctcc tggcggtgag
     3841 cgccgcgact ctcaccgaca cgaaccggat cgggccgctg ccagatgggc cgttgaccgc
     3901 caaggtgcgc tcactggcca ggccggtgcc ggtgacgctc gacggaccat tgggtgaagg
     3961 ggcgacgacg cgcatccgct tcgccgcgcc ggaatttggc gtggctccgg ggcaggcggc
     4021 ggtgatctat gccggggagc gggtggttgg cggcgggtgg atcgagggga ccgaggccgc
     4081 ctgagctagt cgcgcggcca cggctgcagc tggcagcccg cgccttccgt gaacgtggcg
     4141 gtctgcggga aggccagggc aaagcgggca gtgacgctgc gcgccgtctc gtcttcgctc
     4201 agggtgacga ggcccatgcc gccttcgaaa tccttgcgac attggccgag cgggcggcct
     4261 tcgacaaagc ggcacgagca gccgatccgc gccccatagg cgctgccggt gcgggccagg
     4321 gcggcgagtg tcgggccata gagcagcacc gccgccgcga gggcgatcac caccacccag
     4381 agcaagcggc gaaagagcgg ccggcgcggc agggcccggg agtgggcggt ggacgaagcg
     4441 ggtcgggagg ttgccattgc gcgcggttct tcgcatgacc gccccgccca tgtcgacacg
     4501 ctcccgcacg ttcatccgca acctcgcgct ggccagcctg gcgctggtgg caggctgcgg
     4561 ccagcaggcg gcgatgcagg cccctctcga tccggcggcg ctcgctgtcg tcaagccggc
     4621 ggccggggtg ccgcgcgaga agctggcccg ggccatcgat gggctgtttg ccgatcctgc
     4681 cgccggggag acccgcgcgc tgctggtcct ggtggacggg aagatggtgg ccgaacgcta
     4741 tggccccggg ttcgatgcgg gcacccgcca gctcggctgg tcgctgagca agacgctgac
     4801 cgggctgatg atcggcctgc tcgtgtccga tggccggctg cggctggacg agagcgtgcc
     4861 ggtgccagcc tggcagcgca gcggcgatcc ccgcggggag atcacgctac gccagttgct
     4921 gcagatgcgc tcgggcctgc gccatgtcga ggaggcctcg cccgtctaca cttcggacac
     4981 ggcccggctg ctgctcggcg acgggcacga cgataccgcc gcctatgccg aggcccagcc
     5041 gctggccgcc gagccggggc gcaagttcgt ctattccagc gcgaccggag cgatcctttc
     5101 ggacctggcc gcgcgggtac tggccgacgg cgccgatccg gcgttgcggc gccaggtcgt
     5161 gggcgaatac ctgagcacgc ggctgctggt gccgctcggc atggcttcgg ccgtggcgga
     5221 gtacgaccgg gccggcacgc tgatgggcgg cgggatgatc catgcgaccg cacgggactg
     5281 gagcaagctg ggcgagttcc tgcgcaacgg gggcgcggtg cacggcgcgc agatcctgcc
     5341 gcggagctgg gtggagttca tgacccagcc gagcccggcc gagccgggct atggcgcgca
     5401 gctatggcta aaccgcgacc gggcggacgg tgcggaccgg ttgctgcccg gcaagctgcc
     5461 ggcggggatc tttgccgccg tgggccatct cggacagtac gtgatcgtct cgcccgccca
     5521 gaaagtgacg ctggtgcggc tgggccacag cgacaatgcc gaacgccagg ccgttcgcga
     5581 gcggctggcc gaaatcctgc gagtgctgcc gaagggctga tcaggccttg atctgcgcgg
     5641 cggtgagatc cggccgcccg gccaaccccg catcgacgtg gccttccgga tcggggtgaa
     5701 tcaggatctc gacgccgggg aactcgccct ggatcttcgc ctcgatcgca tccatcacgt
     5761 catgggctgc cgcgatggtc atgttgcgat cgacccagac atggaactgg acgaaatcgc
     5821 ggttgcccga ggtgcgggtg cgcagatcgt gcaccccgcg cagctcggga tgacgagcaa
     5881 tgacttcaag gaagcgcttc tttttctcct gcggccattc gcggtccatc agctgctcga
     5941 tcgccgcggt ggatgcgccc caggcacccc agccgagcca ggccgcgatg acgaggccga
     6001 acaggggatc ggctccggcg atgccgaaat actggtcgag cgcgagggcg gcgatgaccg
     6061 cgaggttgag cagcaggtcg gactggtagt gaacatgatc cgtcgagatc gcgaggctgt
     6121 tggtcttgcg gatcacgtgg cgctgccagg cgagcaatcc cagcgtggcg gccatcgcga
     6181 ccgcggaaac gacaatgccc tcgcccgccg ctgccgtccg cccgccggca aagaactgtt
     6241 cgaccgcgcg gaaggccagg ccgagcgccg agatagagat cagcacgacc tggaacatgg
     6301 cggccagcgc ctcggccttg ccgtggccga agcggtggtg aagatcgtcg ggctgggcgg
     6361 cgacccagac cccggcgagg gtggcgaggc tggcgacgag atcgagcgcc gtatcggcga
     6421 ggctgccgag catggcggtc gacccggtct gccaggccgc gaaggccttc atcccgagca
     6481 gaagcgtggc cacgccgatc gaggcgagcg cggcgctgcg gttgaggccg gcggtttgcg
     6541 ggtccgtcat cgcgcgcctt tcagggatag aggagcgtgc tggcccagcc gcagggcgcg
     6601 gcggcatccc ggacgaagcg gcggcgatcg tggaggcgat gctcgcggtc ctgccagaat
     6661 tcgagggcga ccggcctgag gcggaacccg aaccagtggg gtggacgctc gaccgggccg
     6721 tcgccgaaac gggccgcgac ctcgtcgtaa cgggcaagga aggtggcgcg ggaatcgagc
     6781 ggggccgact ggtcggaggc gacggcgccg agctggctgt cgcgcgagcg gctggcgaaa
     6841 taggcatcgg cctcggcgtc gctgacccgt tcgaccgggc cctcgatgcg gatctgccgg
     6901 cgcaggctct tccagtggaa cagcagggcg gcgtgcggat tggcgaccag ttcgccgccc
     6961 ttgcggctgt gcgaattggt gtagaaaacg aagccctccg ggccatgtcc cttgaggagg
     7021 accatgcgaa ccgagggaat gccatccggc gtggccgtgg ccagggccat ggcgttcgaa
     7081 tcgttgggct cggtggtccg cgcctcggca taccatgcat cgaacagggc gaagggatcg
     7141 ccggcgggga gggaagtggt cgcctgttca gagttcaccg cggattcctg acgaagttga
     7201 cgaagttgac actgttcttg gggcgggaaa aatccccttg cccttcaggc ggcggcctcg
     7261 cccctcccat agcccccccg aggcgcgtag gaaagggcgc ctaaggtcga agaggcccac
     7321 ctgtcccttg tgctgtgcca tgggcgcaac tagctagggg ccatggcaga tccatacgcg
     7381 accctggggg tggctcgcgg cgcgagcgaa aaggacatca agtccgccta tcgcaagctc
     7441 gccaaggagc ttcaccccga ccggaacaag gacaacccca aggcagccga gcgcttcagc
     7501 gaggtgaccg gggcctatga cctcctgtcc gacaaggaca agcgggcccg gttcgaccgg
     7561 ggcgagatcg atgccgacgg caatccgacc agcccctttg gcgggggtgg attcggtggg
     7621 ggcggccagc gcggcttccg ccacgacggg ttcgagggtt ttggcggcca gcaggaaggg
     7681 gtggatatct ccgacctgtt cgagggcctg ttcggcggcc gtgccgccgg cggcggcggc
     7741 ggtggcccgt tcggcggtgc gggcggcggc ggctttggcg gtatgggggg cggccggcgc
     7801 gccccggcca agggcgccaa cgtgcagtac cggctcaagg tctcgctgac cgacgcggcc
     7861 atcctcgccg cgcagcggat caccctgtcc gatggcaaga ccatcgatct
//
LOCUS       DATNBV010000043         3635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1619735,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000043 DATNBV010000000
VERSION     DATNBV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619735"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VN627_01065"
     CDS             complement(<1..936)
                     /locus_tag="VN627_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXD01872.1"
                     /translation="MKRTKAIWLAGLGLAAVAPAPAAANSAAVDYFRSRADRTAVPAL
                     LSQDERAYYKDLFGAIEHQDWARVQTLFAQKPDGPLHQVARAEYFLDAKSPRIELDAL
                     SQWLAASSATPLPQTEQVAALAMKRGATALPQLPTAAAFVALPSSPKRLRPRDTNDGT
                     MPALVAAAIQERIKADDPLGARLLLDGIDAGLSAPTRAEYRAKIAWSFYIENDDASAY
                     ALAQTAADGTGPWVAEAWWTAGLSAFRLDDCQGAADAFGRAAGLADNVELKTAALYWQ
                     SRSLVRCRHPEAAAAPLKLAAARDETFYGMLAAEQL"
     gene            complement(933..1712)
                     /locus_tag="VN627_01070"
     CDS             complement(933..1712)
                     /locus_tag="VN627_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01873.1"
                     /translation="MEGLGKRDNDGLRAGDIAGALDWWREAGVDLDFADEAHGWLAAP
                     APPDAPPGPPPGYFAPVGKTPEPVAEPARIGGAAAGLPQDLPAFRQWWLEEPALDDGL
                     VRGRVPPRGEAGAALMIVVGQPDSEDGEELLSGTQGRLLNAMLAAMGIGARDIYLASA
                     LPRTMPVPDWPNLAHRGLGAVLRHHIGLVGPKRLVVFGEGVLSLLGHDPAQNAQNSSE
                     FYHEGKTIALLGARDLTLLARPAWKARFWREWLDWTGTGTE"
     gene            1793..3442
                     /locus_tag="VN627_01075"
     CDS             1793..3442
                     /locus_tag="VN627_01075"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HXD01874.1"
                     /translation="MIERESMPYDVVVVGGGPAGLGTAIRLKQLNPELSVCILEKGSE
                     IGAHILSGATYDPKALDELLPNWRDDGCPMAEVPVTDNWHWNLTKGGKMAIPHIVMPP
                     FLSNHGNYTGSLGNLCRWLGGKAEELGVEIFPGFPAAEVLYGDKGEVIGVQTGDMGVD
                     REGNPKGDFQPGMNLLAKYTVFCEGARGHLTKRLKAKYDLEADCQPQVYGIGMKELWD
                     IDPAKHVPGRVIHTQGWPLSESDSWGGGFLYHQANNQVSLGFVCALDYKNPWVYPFEE
                     FQRWKQHPAIKAILEGGKRVAYGARAINEGGWQSVPKLDFPGGMLAGCSAGFVNVPRI
                     KGSHTAMKSGMLAAEAIAAAIAAGREGDSLGEYDASVRDSWIAKELKLVQNAEPMVAK
                     FGGELGTMLAGADMWLRTIAGFGLAKPMKHHTDASSIERADLYKPIAYPKPDGVISFD
                     RLTSVSYSFTNHEEEQPCHLRLKDPTVPTRINLPLYAGPESRYCPAGVYEFVDPDGSG
                     PRLQINAQNCVHCKTCDIKDPTQNIEWVTPEGGGGPNYPNM"
BASE COUNT          642 a         1235 c         1205 g          553 t
ORIGIN      
        1 aagctgctcg gcggcgagca tgccgtagaa ggtctcatcc cgcgcggcgg cgagcttcag
       61 gggtgcggcc gcggcttcgg gatggcggca gcgcaccagc gagcggctct gccagtagag
      121 cgccgcggtc ttcagctcga cattgtcggc gaggcctgcc gcccggccaa acgcatcggc
      181 cgcgccctgg caatcgtcga ggcggaaggc ggaaagccct gcggtccacc aggcttcggc
      241 cacccatggg cccgtgccgt cagccgcggt ctgcgcaagg gcataggccg aggcatcgtc
      301 gttctcgata tagaacgacc aggcgatctt ggcgcggtat tcggcgcgag tgggggcgct
      361 gaggccggca tcgataccat caagcagcag ccgcgcgcca agcggatcgt cagccttgat
      421 ccgctcctgg atcgcggcgg cgaccagcgc gggcatggtg ccgtcattgg tgtcgcgggg
      481 gcgcaggcgc ttggggctcg agggcagggc aacaaacgcc gccgcggtcg gcagttgggg
      541 cagggcggtg gccccgcgct tcatcgccag ggcggcgacc tgctcggttt gcggcagcgg
      601 ggttgccgag ctggccgcga gccactggct cagcgcatcc agctcgatcc tggggctctt
      661 ggcgtcgagg aaatattcgg cccgggccac ctggtgcagc gggccatcgg gcttctgggc
      721 gaacagggtc tgcacccgcg cccagtcctg atgctcgatc gcgccgaaca gatccttgta
      781 atacgcgcgc tcgtcctggg agagcagggc ggggacggcc gttcggtccg cccggctgcg
      841 gaaatagtcc accgccgcgc tgttcgccgc cgcaggtgcg ggcgccacgg cggccagtcc
      901 aaggcccgcc agccagatcg ccttggtccg cttcactcgg ttccggttcc cgtccagtcc
      961 agccattccc gccagaagcg ggccttccat gccggccgcg ctagcaatgt caggtcgcgc
     1021 gcccccagca gcgctatggt tttaccctca tggtaaaact cagatgaatt ttgagcattt
     1081 tgcgccggat cgtggcccaa gagcgataga acgccctcac cgaacaccac cagtcgtttc
     1141 ggccccacca gtccgatgtg gtggcgcagc accgcgccga ggccgcgatg ggcaaggttt
     1201 ggccagtccg gcaccggcat ggtccggggc agggcgctgg ccaggtagat gtcccgggcg
     1261 ccgattccca tcgcggccag catggcgttc agcagccggc cctgggtacc ggagagcagt
     1321 tcctctccgt cctcgctgtc cggctggccg acgacgatca tcagcgcggc gcccgcttcg
     1381 ccgcgaggcg gcacgcgccc gcggaccagg ccatcgtcga gcgcgggttc ctccagccac
     1441 cattggcgga aagcaggaag gtcctgcggc aagcccgccg cagccccgcc gatccgagca
     1501 ggctcggcca ccggctcggg cgtttttccg acaggggcga aatatccggg agggggaccg
     1561 gggggcgcgt cgggcggcgc aggggcggcc agccagccat gcgcctcgtc ggcgaaatcg
     1621 aggtcgaccc cggcctcgcg ccaccagtcc agcgccccgg caatatcgcc ggcgcgcagg
     1681 ccatcattgt cgcgtttgcc aagaccctcc atgaccagag gccttgacct gccggagaag
     1741 tgcaatcaag gcactttcga tcgaaagtgt gcaggacagg acgggttttc ccatgatcga
     1801 acgcgaatcc atgccgtatg acgtcgtcgt cgtcggcggc ggcccggcgg ggcttggcac
     1861 tgccatccgg ctcaagcagc tcaatcccga gctctcggtg tgcatcctcg agaagggcag
     1921 cgagatcggc gcgcacatcc tttcgggtgc gacgtacgat cccaaggcgc tcgacgaact
     1981 cctgccgaac tggcgcgacg acggctgccc gatggccgaa gtgccggtga ccgacaactg
     2041 gcactggaac ctgaccaagg gcggcaagat ggcgatcccg cacatcgtca tgcccccgtt
     2101 cctgtcgaac cacggcaatt acaccggttc gctcggcaac ctgtgtcgct ggctcggcgg
     2161 caaggccgag gagcttggcg tagaaatctt ccccggcttc cccgccgccg aagtgctcta
     2221 tggcgacaag ggcgaagtca tcggcgtgca gaccggcgac atgggggttg accgcgaagg
     2281 caatcccaag ggcgatttcc agccgggcat gaacctcctg gccaagtaca ccgtgttctg
     2341 cgagggcgcg cgcggccacc tcaccaagcg cctgaaggca aagtacgacc tcgaggccga
     2401 ttgccagccg caggtctacg gcatcggcat gaaggagctg tgggatatcg atccggccaa
     2461 gcacgtgccg ggccgggtga tccacaccca gggctggccg ctgtcggaaa gtgatagctg
     2521 gggcggcggc ttcctctatc accaggccaa caaccaggtc tccctgggct tcgtctgcgc
     2581 gctcgattac aagaacccct gggtctatcc gttcgaggaa ttccagcgct ggaagcagca
     2641 tccggcgatc aaggcgatcc tcgaaggcgg caagcgcgtc gcctatggcg cccgcgccat
     2701 caacgagggc ggctggcagt cggtgccgaa gctcgatttc cccggcggca tgctcgctgg
     2761 gtgctcggcc ggcttcgtca acgtgccgcg catcaagggc agccacaccg cgatgaagag
     2821 cgggatgctc gccgccgaag cgatcgccgc agccatcgcc gccggtcgcg agggcgacag
     2881 cctgggcgag tatgacgcct ccgtgcgcga cagctggatc gccaaggagc tcaagctggt
     2941 ccagaacgca gagccgatgg tcgccaagtt cggcggcgaa ctgggcacga tgctggctgg
     3001 cgccgacatg tggctgcgca ccatcgccgg cttcggcctt gccaagccga tgaagcacca
     3061 caccgacgcc tccagcatcg agcgcgccga tctctacaag ccgatcgcct atcccaagcc
     3121 cgatggcgtc atcagcttcg accggctgac cagcgtctcc tactcgttca ccaaccacga
     3181 ggaagagcag ccctgccatc tacgcctcaa ggacccgact gtacccacgc gcatcaacct
     3241 gccgctctat gctggcccgg aatcgcgcta ttgcccggcg ggcgtctacg aattcgtcga
     3301 cccggatggc agcgggccgc ggctccagat caacgcccag aactgcgttc actgcaagac
     3361 ctgcgacatc aaggacccca cccagaacat tgaatgggtc acgcccgaag gcggcggcgg
     3421 gccgaactat ccgaatatgt aatcctcccc gaaacgggga gggggaccac cgaaggtggt
     3481 ggaggggcac aggcgagtta tcccaacgct gcatgacgaa ccgaaagccc ctcccgtgat
     3541 caccggcccg aaggcaacgc tcaagctcgc gcgcaaactg cgcagtgaga tgaccttgcc
     3601 cgaaacgctg ttgtggcgcg aactaccgct gcggc
//
LOCUS       DATNBV010000044         5368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1301573,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000044 DATNBV010000000
VERSION     DATNBV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301573"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VN627_01080"
     CDS             <1..1038
                     /locus_tag="VN627_01080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXD01875.1"
                     /translation="APTPQKTWNFPSMTIPAISVAMSVFNGERFLAPAIESVLTQTFG
                     DFEFLILDDGSSDCSGTIIGDYARRDSRIRPIQRENRGLVASLNQLLAEARAPLVARM
                     DADDICKPERFARQIAFLAGHPDHGVVGSWSEDIDEHGAPYKVSGPEHPDTHERFLLA
                     IENGWPLLCHPAVMYRRDLVRSVGGYHAAFRHCEDLDLWLRLASVTRLCSLPERLIRY
                     RHYAGQVSSRHATEQQVGAAVARMAWEERRAGRPDPTADLTTLPPIEDLNTLFGRPDA
                     ASHVRAQVAPGLLYSRAGLGDHGFELVVDYLREGGARDGMWRTVARLVRFGEPVRAVK
                     LAATLARTMAA"
     gene            1041..2552
                     /locus_tag="VN627_01085"
     CDS             1041..2552
                     /locus_tag="VN627_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HXD01876.1"
                     /translation="MAAVSETGAREPAAMGVRSAALWSMAGQYAAFAIQFGASVVISR
                     FFLGPAEVGLFSIALAAALIVAILQDFGLSRYIAGLPRLNGTEISRCSSVALLFSLVV
                     AGLIAAAAWPLARLYALPSLLPLMLVIAASYLFLPLSVVPMALMARAMQFRGLFLVNV
                     GSALTQALVAVGLAAAGNSSSALAWATLAAALARGATAQALRPSPPWPLRLDGVRQVV
                     RFGSRSSGLYLTGALGTRTPDLIVGKLLTLAAVGMYSRAVSLADQFRMLVSGAIGSVF
                     YPAFARIRDRGEPLGPAYLRVCAGYSAVLWPGMAGLALAAEPIVRLLYGEAWAGVVPL
                     LQRIALLELFVVTLPLHIDLPILVGRMNQLLLLNFVDTVLSIGLLALGCLWGVEGAAA
                     SRLIYGALWIALYARFMHEVVGFEPRRLLGIYLRSGMATLAALAPLFLAYRYWLPADQ
                     IGLVPLALAAAAGVLAWLAMLAATRHPAFADLTGIAREALAPFDRRRRMPLPG"
     gene            2564..3397
                     /locus_tag="VN627_01090"
     CDS             2564..3397
                     /locus_tag="VN627_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HXD01877.1"
                     /translation="MTQVFLILAHEKADQLSALIERLAPADGPNRAVVHIDRKSPLWR
                     ETHGRFLAGNSNVTLVARPVAVRWGHASQLAATRLLLRAALARPFDLAHLISGADWPV
                     VPPAAMAASFAGACHIEVVPGVQSERMKRIRLDARMLRPDPGRPSEWYPARTLRHLSA
                     LLPARRLQPWGPWQKGSQWWSLPRDVCEVVLAGLDRAFATGLLAGTVCADEHLIQTIV
                     AHQFPDRIATNRRFLRWGEGASPAILGAGDLPEILASGAWFARKVSAEADDFFHTLPS
                     S"
     gene            3452..5272
                     /locus_tag="VN627_01095"
     CDS             3452..5272
                     /locus_tag="VN627_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HXD01878.1"
                     /translation="MPPHTATNAPGARHDGWNTLSRFLPYLWPRDNPALRWRIVVSSL
                     LILASTAVQLSLPYLLKWAVDAMNVTGPRLPAFVMLTVLGYSAGRFLGVGFDNLRNIV
                     FERVGQDATRALAENVFSRLHKLSMRFHLARRTGEVTKVIERGTKSIDTMLYFMLFNI
                     APTVLQLAIVAVIFYVNFGWGLVAATGAAVVAYIYVTRAITEWRTHLREKMNRLDGQA
                     LARAVDSLLNYETVKYFVAERREEARYAQATRAYADAAVKSENSLGLLNIAQALVVNL
                     LMAGALAWTVWGWAQGKYSAGQLVFVQTYLTQLFRPLDMLGMVYRTIRQGLIDMAAMF
                     KLIDEPQEVADAPGAPALVVRRPAVTFDNVVFGYDPDRTILHGLSLEIPAGRQVAIVG
                     PSGAGKSTIGRLLFRFYDPTAGRILIDGQDIKLVTQQSLRSAIGIVPQDSVLFNESIG
                     YNIAYGRDGATEADVEAAARAAALSDLIGRLPKGFATEVGERGLKLSGGEKQRVAIAR
                     TLVKNPPILLLDEATSALDTRTEQDILATLHAAAENRTTISIAHRLSTIADADTIFVL
                     DQGRLAEQGTHGDLLRRDGLYAEMWQRQAAEKEELSEAAE"
BASE COUNT          767 a         1942 c         1700 g          959 t
ORIGIN      
        1 gctccgaccc cgcagaagac ctggaatttc ccgagcatga ctattcccgc gatcagcgtc
       61 gccatgagcg tcttcaacgg cgaacgcttt ctcgcacctg caatcgagag cgtgctcacc
      121 cagaccttcg gcgacttcga attcctgatt ctcgacgacg gctccagcga ttgctcggga
      181 acgatcatcg gggactacgc ccggcgcgat tcccgtatcc ggccgataca gcgcgagaac
      241 cgcggcctcg tggcgagcct gaaccagctg ctcgccgaag cgcgtgcccc gctggttgca
      301 cggatggatg ccgacgacat ctgcaagccg gaacggttcg cccgccagat cgccttcctc
      361 gccggccatc ctgatcatgg cgtcgtcggt agctggagcg aagacattga cgagcacggc
      421 gcaccttaca aggtcagcgg ccccgagcac cccgacaccc acgaacggtt tctgctcgcc
      481 atcgagaacg gctggccgct gctgtgccac ccggccgtca tgtaccggcg cgacctggtc
      541 cgctcggtcg gcggctatca tgccgccttc cgtcactgcg aagacctgga cctgtggctg
      601 cggctcgcgt cggtcacccg cctgtgctcg ctgccagagc ggctgatccg ctatcgccac
      661 tacgccgggc aggtctccag ccgtcacgcg accgagcagc aggtcggggc tgccgtggcc
      721 cgaatggcct gggaggaacg ccgggccggt cggccggacc cgacggcgga tcttaccacc
      781 ctgcccccga tcgaggatct caataccctg ttcggccggc ccgatgcggc cagccatgtg
      841 cgggcccagg ttgcccccgg gctgctctat tcgcgcgccg ggctcggcga ccacgggttc
      901 gaactcgtgg tcgactacct gcgggaggga ggagcgcgag acggaatgtg gcgcaccgtg
      961 gcacggctcg tgcggttcgg ggagccggtt cgcgccgtca agctggccgc caccctcgcc
     1021 cgcaccatgg ctgcctgacc atggctgcag tgagcgaaac cggcgcgcgc gaacccgccg
     1081 ccatgggagt gcgcagcgca gctctctggt ccatggccgg ccaatacgct gccttcgcta
     1141 tccagttcgg cgcgtctgtc gtgatctcgc ggttttttct cggcccggca gaagtcggcc
     1201 tgttttccat cgcgctcgca gccgcactga tcgttgcgat cctccaggac ttcggccttt
     1261 cacgctacat cgccggcctg ccccggctca acggcaccga gatctcccgc tgcagcagtg
     1321 tcgccctgct gttctcgctg gtcgttgccg gactgatcgc cgccgccgcc tggccactcg
     1381 cgcggctcta tgcgcttccg tcgctgttac cgctgatgct cgtcatcgcc gccagctacc
     1441 tcttcctgcc gctaagtgtg gtgccgatgg cgctgatggc gcgggccatg cagttccggg
     1501 gcctgttcct ggtcaacgtc ggctcggcgc tcactcaggc actggttgcc gtgggtcttg
     1561 ccgccgccgg caattcttcg tccgctctgg cctgggccac cttggccgcc gctctcgcca
     1621 ggggcgccac ggcgcaagca ctgcggccgt ccccgccctg gccgctgcgc ctcgacggcg
     1681 tgcgccaggt cgttcgcttc ggtagccgct cgagcggact ctatctcacg ggcgccctcg
     1741 gtacgcgcac acccgacctc atcgtcggca agctgctgac cttggctgca gttggcatgt
     1801 atagccgcgc ggtctcgctt gccgaccagt tccgcatgct cgtcagcgga gccatcggca
     1861 gcgtattcta tcccgctttc gcccgcatcc gcgatcgcgg cgagccgctc ggccccgcct
     1921 acctcagggt ctgtgccgga tatagtgccg tgctctggcc aggcatggca ggcctcgctc
     1981 tcgcggcgga acccatcgtg cgcctgctgt atggcgaagc gtgggccggc gtcgttccgc
     2041 tgctccagcg gatcgccctg ctcgagctgt tcgttgtgac cctgccgctt cacatcgacc
     2101 tgccgatcct tgtcggccgg atgaaccagc tgctcctgct caatttcgtc gacacggtgc
     2161 tgtcgatcgg cctcctcgcc ttgggctgcc tgtggggggt ggagggcgcg gcggcctctc
     2221 gtctgatcta tggcgcgctc tggatcgcac tttacgcccg attcatgcac gaggtggtcg
     2281 gctttgagcc gcgccggctc ctgggtattt acctgcgcag cgggatggcc acgctggccg
     2341 cactggctcc gctgttcctg gcttaccgct actggctccc ggccgaccaa atcgggctgg
     2401 tgcccctcgc gctcgccgcc gccgccgggg ttctcgcctg gctggcgatg ctcgccgcca
     2461 cccgccatcc ggccttcgcc gacctcaccg gaatcgcccg cgaggcgctg gccccgttcg
     2521 accggcgccg gcgcatgccg ctgccgggct gacctccgcc accgtgaccc aggtgtttct
     2581 gatcctcgcc cacgagaagg ccgaccagct cagcgcgctc atcgaacgcc tcgcgccagc
     2641 ggacgggccg aaccgggccg tggtccacat cgatcgaaaa agcccgctgt ggcgcgagac
     2701 ccatggccgg ttcctggccg gcaattcgaa cgtgaccctt gtcgcccgtc ccgtcgcggt
     2761 gcgctggggc catgccagcc agctcgccgc aacccgcctg ctgctacgcg ccgcgcttgc
     2821 ccggccgttc gacctcgccc accttatcag cggggccgac tggccggtcg ttccaccggc
     2881 ggcgatggcc gccagcttcg ccggtgcgtg ccacatcgaa gtcgttccag gagtccagag
     2941 tgagcgaatg aaacgcatcc gcctggacgc gcggatgctt aggcccgatc ctgggcgtcc
     3001 gtctgaatgg taccccgccc gcaccctgcg ccacctctcg gccctgctcc cggcgcgccg
     3061 cctccagccg tgggggccgt ggcaaaaagg ctcgcaatgg tggtcactgc cgcgcgatgt
     3121 ttgcgaggtc gtcctcgccg ggctcgaccg cgcctttgcc acgggccttc ttgcggggac
     3181 agtctgtgcc gatgagcacc tgatccagac catcgttgcc caccagtttc ccgaccggat
     3241 tgcgaccaat cgaaggttcc tgcgctgggg cgagggcgcc agtccggcaa tccttggcgc
     3301 gggcgatctg ccagaaatcc tcgccagcgg cgcctggttc gcccgcaagg tgtcggccga
     3361 ggcggacgat ttcttccaca cgcttccctc cagctgaatt cggtgggtgg gcgcctttcc
     3421 atttgctgca acgcacctac ataggcgctg catgcccccc cataccgcca ccaacgcccc
     3481 cggcgcccgc cacgacggct ggaacacgct cagccggttc ctgccctacc tgtggccgag
     3541 ggacaatccg gcgctgcgct ggcggatcgt cgtctcctcg ctgctgatcc tcgcctcgac
     3601 cgcggtgcag ctttcattgc cctacctgct caagtgggcg gtcgatgcga tgaacgtgac
     3661 ggggccgcgc ctgccggcct tcgtcatgct gacggtgctc ggctattcgg cgggacggtt
     3721 cctcggcgtg ggcttcgaca acctgcgcaa catcgtgttc gaacgggttg gccaggacgc
     3781 gacccgggct cttgcggaga acgtgttctc ccgcctccac aagctgtcga tgcgcttcca
     3841 tctcgcccgc cgcaccggcg aggtcaccaa ggtgatcgag cgcgggacca agtccatcga
     3901 cacgatgctc tacttcatgc tgttcaacat cgcgccgacg gtgctgcagc tcgccatcgt
     3961 cgcggtgatc ttctacgtca atttcggctg gggcctggtc gccgcaacgg gggcggcagt
     4021 tgttgcctat atctacgtca cccgcgcgat caccgagtgg cgcacgcatc tgcgcgagaa
     4081 gatgaaccgt ctcgacgggc aggcgctggc gcgggcggtc gactcgttgc tgaactacga
     4141 aacggtgaag tacttcgtgg ccgagcgccg cgaggaagcg cgctatgccc aggccacccg
     4201 cgcctatgcc gatgccgcgg tcaagagcga gaacagcctc ggcctgctga acatcgccca
     4261 ggccctggtg gtgaacctgc tgatggccgg ggccctcgcc tggaccgtgt ggggctgggc
     4321 gcaaggcaag tattcggccg gccagctggt cttcgtccag acctacctga cccagctgtt
     4381 ccgcccgctc gacatgctcg gcatggtcta ccgcacgatc cgccaggggc tgatcgacat
     4441 ggccgccatg ttcaagctga tcgacgagcc ccaggaagtg gccgacgcgc cgggcgcccc
     4501 ggcgctggtg gtgcgccgcc ccgcggttac cttcgataac gtggtcttcg gctacgatcc
     4561 tgaccgcacg atcctccacg gcctcagcct cgaaatcccc gctgggcggc aggtggccat
     4621 cgtcggcccc tcgggcgcgg gcaagagcac gatcgggcgg ctgctgttcc ggttctacga
     4681 tcccacggca ggccgcatcc tgattgacgg gcaggatatc aagctcgtca cccagcaatc
     4741 gctgcgctcg gccatcggca ttgttccgca ggattcggtg ctgttcaacg aatccatcgg
     4801 ctacaatatc gcctatggcc gtgacggagc gaccgaggcc gatgtcgagg ccgccgcgcg
     4861 cgccgccgct ctgtccgatc tgatcggccg cctgcccaag ggcttcgcca ccgaagtggg
     4921 cgaacgcggg ctcaagctct cgggtgggga gaagcagcgc gtggccattg cccgcacgct
     4981 ggtgaagaac ccgccgatcc tgctgctgga tgaagccacc agcgcgctcg acacgcgcac
     5041 cgagcaggac atcctcgcca cgctgcacgc cgcggccgag aaccgcacga cgatctccat
     5101 cgcccaccgc ctgtccacca ttgccgatgc cgacacgatc ttcgtactgg atcaggggcg
     5161 gctcgcggag cagggaaccc acggcgacct gctgcggcgc gacgggctct atgccgagat
     5221 gtggcagcgt caggcggcgg agaaggaaga gctgagcgag gcggcggagt agcgcgggac
     5281 gcgcatcgcg catcggcaca ccccctccgt cagccgattt gcggctgcca cctccccacc
     5341 agtgcggagg atttcctaag cctgcagc
//
LOCUS       DATNBV010000045         2295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_671135,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000045 DATNBV010000000
VERSION     DATNBV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671135"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VN627_01100"
     CDS             <1..824
                     /locus_tag="VN627_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HXD01879.1"
                     /translation="ELGQALDPSNSLYRPGGHPAQVPAAGGGGRAEDLGSVPTQVVPT
                     AVTPTGPGDLDLDLDFSIGDDEPAPPPVRASAAALPGAAVDLPPVVEPMPSLDMDFGS
                     AVQPPAAAEPATARAEPAGPDGPDLDVSDKGLSFDATQTMKAAPVKAAPAPAPDAGMI
                     EFDLGALSLDLDSPPAAVAPATPAAPAAVAPAAPTASKAPAEEAFDSTAGAPHSTGGL
                     GAEDNSDPLATKLALAQEFNAIGDPDGARSLAEEVLAEASGDLKQKAQRFLAEIA"
     gene            864..1646
                     /gene="truA"
                     /locus_tag="VN627_01105"
     CDS             864..1646
                     /gene="truA"
                     /locus_tag="VN627_01105"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXD01880.1"
                     /translation="MRLALGISYNGQAYEGWQSQPSGRTVQDRLEAALSQFAVGPITT
                     VCAGRTDAGVHGLMQVVHFDTTLRREEFSWIRGTNRFLPADIAVQWAKPVPDAFHSRA
                     SAVARRYAYVVLESPVRPSIDAGRVGWVFRPLDGGAMQEAAALLIGQHDFSSFRAAAC
                     QARTPVKTMNRVTIARHGSYWRFEFEADAFLHHMIRNIMGCLLVIGQGQQPPQWMSQV
                     LAARDRDAAAPTFSPDGLYFLGPVYSPEWKLPSRTAAYDWLP"
     gene            1643..>2295
                     /locus_tag="VN627_01110"
     CDS             1643..>2295
                     /locus_tag="VN627_01110"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HXD01881.1"
                     /translation="MNVANPLFHRTRIKICGLTREPDVDAAVAAGADAVGFVMYRKSA
                     RYVSPQRAAELARRLPPFVTPVLLFVNESAAVVLAACEQVAGSIVQFHGDETPAQCLE
                     ATAGGKHPFMRVARIELDETTPFDLLEFAARYPQAQAIMLDAHVEGYGGGGKAFDWSR
                     IQVPSAAAGPVRTGAVLPHLVLSGGLNAANVTDGIVQVRPRCKTLAVDVSSGVELDGP
                     "
BASE COUNT          366 a          794 c          751 g          384 t
ORIGIN      
        1 gcgaactcgg acaggctctc gaccccagca attccctcta ccggcccggc ggccaccccg
       61 cccaggtgcc ggcggctggt ggcggcgggc gcgccgagga tttgggctcg gtacccacgc
      121 aggtcgtgcc gaccgccgtc acgccgacag gacccggcga tctcgacctg gaccttgatt
      181 tttcgatcgg cgacgacgag cccgctccgc cccccgtcag ggcctccgcg gctgcccttc
      241 ctggcgccgc ggtggacctg ccacccgtgg tcgagcccat gccctctctg gacatggatt
      301 tcggttcggc cgtacagccg cctgcagctg ctgagcccgc caccgcgcga gcggagcccg
      361 ccggcccgga tggacccgat ctggacgtgt ccgataaggg cctgagcttc gacgctaccc
      421 agacgatgaa agcggcgccc gtgaaggcgg ctcccgcgcc ggctccggac gccggaatga
      481 tcgagttcga cctgggcgcc ttgtcactgg acctcgatag tccgcccgcc gccgttgcgc
      541 ctgccacgcc ggcagcgcct gctgccgtcg cgcctgctgc gccgacagcc agcaaggcgc
      601 cggccgaaga ggctttcgac agcacggccg gtgcaccgca cagcacggga ggcctcgggg
      661 cagaggacaa cagcgacccc ttggcaacca agctcgcctt ggcgcaggag ttcaatgcca
      721 tcggcgaccc ggacggtgct cgcagcctgg ccgaagaagt cctggccgaa gcatcggggg
      781 acctgaagca gaaggcccag cgattcctcg cggaaatcgc ctgatccctt cgcgtccgcg
      841 agccaatcgg tgaaagcccg ccaatgaggc tggcgctggg catcagctac aacggacagg
      901 cctatgaagg ctggcaaagc cagccttcgg gccgcaccgt gcaagaccgg ctggaagccg
      961 ccctcagtca attcgccgtt gggccgatca ccacggtttg tgccgggcgt accgatgccg
     1021 gggtgcacgg cctgatgcag gtcgtgcact tcgacactac cctgcggcgc gaggagttct
     1081 cctggatccg cggcaccaac cgctttctgc cggccgatat cgcggtgcag tgggccaagc
     1141 cggttcccga tgcgttccat tcgcgcgcca gcgctgtggc gcgccgctat gcgtatgtgg
     1201 tgctggagtc gccagtgcgc cccagcatcg atgccggccg cgtgggttgg gtgttccgcc
     1261 cactcgacgg cggcgcgatg caggaagccg cagcgctgct gatcggccag cacgatttca
     1321 gctcgtttcg agccgctgcc tgccaggcca ggacgccggt caaaaccatg aaccgggtga
     1381 caatcgcccg ccatggcagc tactggcgct tcgagttcga ggcggacgcc ttcctgcacc
     1441 acatgatccg caacatcatg ggttgcctgc tggtgatcgg ccaaggccag cagccgccgc
     1501 aatggatgag ccaggtattg gcggcacgcg accgcgatgc tgccgcgccc acgttttccc
     1561 cggatggcct gtattttctg ggtccggttt acagcccgga gtggaaactt cccagccgca
     1621 cggctgcgta tgattggctg ccatgaatgt ggcgaatccg ctgttccacc gcacccgcat
     1681 caagatctgc ggactgacgc gcgagccgga tgtggacgct gcggtggcgg cgggcgccga
     1741 tgccgtcggg ttcgtgatgt accgcaagag cgctcgctac gtctcgccgc agcgggcggc
     1801 cgagctggcg cgccgcttgc cgcctttcgt cacccccgtc ctgctgttcg tgaatgaatc
     1861 tgccgcggtc gtcctggccg catgtgagca agtggcggga tccatcgtcc aattccatgg
     1921 cgacgagacg ccggcgcagt gcctggaagc caccgccggc ggcaagcatc ccttcatgcg
     1981 cgttgcccgg atcgaactcg atgaaaccac accgttcgac ttgctagaat tcgcggctcg
     2041 atatccacaa gcccaggcca tcatgctcga cgctcatgtc gaggggtatg gcggcggcgg
     2101 gaaagccttc gattggtcac gtattcaagt ccccagcgca gccgcaggcc cggtacggac
     2161 cggcgccgtg ctgcctcacc tcgttttgtc tggtggactc aatgcggcaa acgtgaccga
     2221 tggcatcgtg caggtgcggc cgcgctgtaa gacgctggcc gtcgatgtga gctccggcgt
     2281 cgagctcgat ggccc
//
LOCUS       DATNBV010000046         2686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3215556,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000046 DATNBV010000000
VERSION     DATNBV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3215556"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VN627_01115"
     CDS             <1..561
                     /locus_tag="VN627_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXD01882.1"
                     /translation="GNPDAPIKLVEYGSLTCPHCGELSEKGSATIRDQWVASGRVSYE
                     FRHFVRDALDLTLGLMASCGGPQTYFGQVEQVFANQKSIFDGLQGKDAQMQAAMKASD
                     AQRFTAIADVAGLPQFFAARGIPAAQAKTCLANTKLAEQMAKNTTDQGSQYQIEGTPT
                     FLVNGDKIGTSTWEELKARIEGMGVR"
     gene            591..>2686
                     /locus_tag="VN627_01120"
     CDS             591..>2686
                     /locus_tag="VN627_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXD01883.1"
                     /translation="MQIRRMKLSGFKSFVEPAELRIEPGLTGVVGPNGCGKSNLLEAI
                     RWVMGESSAKSMRGGGMEDVIFAGTTTRPARDFAEVVLHASDSAGEDLEVVRRIERGA
                     GSAYRVNGRDVRAKDVALVFADAATGAHSPALVSQGRIAAVIAAKPAERRQMLEEAAG
                     IAGLHVRRRDAEQKLRATEANLARLEDILGQLDQQIGTLRRQARAAERYTAVSDRIRV
                     AEARLVFARWRDAAAAADAARKEAQGAEQAVTTAQGLAAEAQKRQHAAAAALVEARDE
                     LADRRDDTTAHAHRLAALAGQLEAAQQRLADLDRQRRRLDEDKGDADRLTQDAAQALA
                     RLDADLAAGEAALEAAAAERPALAREVERAEAQARTAELALAQATAEQAGADAEWRVA
                     EAEVTQARARLARLEADARRQDDQRAALSREGDPEAAVTAALAERDAATARLSDLRID
                     LEARQSRKAELTAARDEAASALSAARADLAGVEREWTALTRDREARAKQASARHGLPT
                     ALDKVRAEPGYERALAAVLGRDAKSGLGTPAPGSEGRFWTGAPAPAAVPQSLAAHVPA
                     CPDELKARLALVHVAPEDDARALAPGEWLVTTGGTLRRWDGFVARGEGAAEAARLEAD
                     NRFAALDAQVPALRAAVAAAEAAQAASAAALTALQTELIAAERSLAGAAERERAALRA
                     LDQAEAAKARLAARRA"
BASE COUNT          420 a         1103 c          882 g          281 t
ORIGIN      
        1 ggcaaccccg atgcgccgat caagctggtc gaatatggct cgctcacctg cccgcactgc
       61 ggcgagctgt cggaaaaagg ctcggccacg atccgcgacc agtgggtcgc ctcgggccgc
      121 gtctcctacg aattccgcca cttcgtccgc gatgcgctcg atctcacgct cggcctgatg
      181 gccagctgcg gcgggccgca gacctatttc ggccaggtcg agcaggtctt cgccaaccag
      241 aagtccatct ttgacgggct ccagggcaag gatgcccaga tgcaggcggc gatgaaggcc
      301 tcggacgcac agcgcttcac ggccatcgcc gatgtcgccg gcctgcccca gttcttcgcc
      361 gcgcggggca ttccggcggc ccaggccaag acctgcctcg ccaacaccaa gctggccgag
      421 caaatggcca agaacaccac cgatcagggc agtcagtacc agatcgaggg cacgccgacc
      481 ttcctcgtca acggtgacaa gatcggcacc tccacgtggg aggaactgaa ggcccggatc
      541 gaggggatgg gcgtccggta agccggagga tacgggccag gggggccgca atgcagatca
      601 ggcggatgaa gctcagcgga ttcaagagct tcgtcgagcc tgccgagctg cgcatcgaac
      661 ccggcctcac cggcgtcgtc ggccccaacg gctgcggcaa gtccaacctg ctcgaagcca
      721 tccgctgggt catgggcgaa tccagcgcca agtccatgcg cggcggcggc atggaagacg
      781 tgatcttcgc cgggacgacc acgcgcccgg ctcgcgattt cgccgaagtc gtcctccacg
      841 ccagcgacag cgcgggcgaa gacctcgagg tcgtccgccg gatcgagcgt ggggcaggca
      901 gcgcctaccg ggtcaacggc cgcgatgtcc gcgccaagga cgtcgccctc gtcttcgccg
      961 atgccgccac cggggcccac agcccggccc tcgtcagcca gggccgcatc gccgcggtca
     1021 tcgccgccaa gccggccgag cgccgccaga tgctggagga agccgccggc atcgcgggcc
     1081 tccacgtccg tcgccgcgat gccgagcaaa agctgcgcgc caccgaggcc aacctcgccc
     1141 ggctggagga catcctcggc cagctcgacc agcagatcgg cacgctgcgc cgtcaggcac
     1201 gagccgctga acgctacacc gccgtttccg accgtatccg cgtcgccgaa gcccgcctcg
     1261 tcttcgcccg ctggcgcgat gccgccgccg ccgccgatgc cgcccgtaag gaggcacaag
     1321 gcgccgaaca ggccgtcacc accgcgcagg gcctcgccgc agaggcccag aagcgccagc
     1381 acgccgccgc cgccgccctg gtggaggcgc gcgacgaact cgccgaccgg cgcgacgata
     1441 ccacggccca cgcccaccgc ctcgccgccc tcgccggcca gctcgaagcc gcgcagcagc
     1501 gcctcgccga cctcgaccgc cagcgccgcc ggctcgacga ggacaagggc gatgccgacc
     1561 ggctgacgca agacgccgcc caggcgctcg cccggctgga cgccgatctc gccgccggcg
     1621 aggccgcgct ggaagccgcc gcggcggagc gcccggcgtt ggcccgcgag gtcgagcgcg
     1681 ccgaagccca ggcccgcacc gccgaactgg cgctggccca ggccaccgcc gagcaggccg
     1741 gcgccgatgc cgaatggcgc gtcgccgaag ccgaagttac ccaggcccgc gcccgcctcg
     1801 cccgccttga agccgatgcc cgccgccagg atgaccagcg cgccgccctg tcccgcgaag
     1861 gcgatcccga agccgccgtc accgccgccc tcgccgaacg tgacgccgcc accgcgcgcc
     1921 tgtcagacct gcgcattgat ctcgaagccc gccagtcccg caaggccgaa ctcaccgccg
     1981 cgcgtgacga ggccgccagc gcactatccg ccgcccgcgc cgacctggcg ggcgtcgagc
     2041 gcgagtggac cgccctcacc cgcgaccgcg aggcccgcgc caagcaggcc agcgccaggc
     2101 acggcctccc cacagcgctc gacaaggtcc gcgccgaacc gggctacgaa cgggcgctgg
     2161 ccgcagtcct cggccgcgac gcgaagtcgg gcctcggcac ccccgcgccg ggcagcgaag
     2221 gccgcttctg gaccggcgcc ccggcccccg ccgccgtgcc gcaaagcctc gccgcccacg
     2281 tccccgcctg cccggacgaa ctcaaagccc gcctcgccct cgtccacgtc gctcccgagg
     2341 acgacgcccg cgccctcgcc cccggcgaat ggctggtcac cacgggcggc accttgcgcc
     2401 gctgggacgg cttcgtcgct cgcggcgaag gcgccgccga ggccgcccgg ctcgaggccg
     2461 acaaccgctt cgccgcgctc gacgcgcagg tgcccgccct gcgcgccgcc gtcgccgccg
     2521 cggaagctgc ccaggccgcc agcgccgccg cgctcaccgc cctgcagacc gagctcatcg
     2581 ccgccgaacg cagcctcgcc ggcgcggccg agcgggaacg cgccgccctg cgcgcgctcg
     2641 atcaggccga ggccgccaag gcccgcctcg ccgcccgccg cgccga
//
LOCUS       DATNBV010000047         2775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1304782,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000047 DATNBV010000000
VERSION     DATNBV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304782"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1894)
                     /locus_tag="VN627_01125"
     CDS             complement(146..1894)
                     /locus_tag="VN627_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HXD01884.1"
                     /translation="MTDTTATLVPTPNEDRLERRFSDFATFGDALDYAAKGARGLNFH
                     DARGNLSRVYPFAELREDALAVARRLVAQGVKPEDRIALIAETGPEFAALFCGTIYAG
                     AWPVPLPLPTSFGGKDNYIEQLVVQLNSSDPSILFFPGEIAEMAGAAAARQGCAGIAF
                     ENFLADPAPAGVALPAASSDQICYLQYSSGSTRFPHGVAVTHEALLNNLAGHGHGMKL
                     IQTDRCVSWLPWYHDMGLVGCFLSPIANQVSADYLKTEDFARRPLAWLDMISRNPGTT
                     LSYSPTFGYDICARRISSQSHVAERFDLSRWRIAGNGADMIRPDVMQGFVNAFAEAGF
                     KAAAFLPSYGLAEATLAVTIMPPGEGIRVELVEEERLSGCPRDLSRPARYRAIVNCGK
                     PVRDMVVEVRGERGLPLADHHIGKVWCKGPSVMHSYFRDPESTEACLVDGWLDTGDMG
                     YLAEGYLFIVGRAKDMIIINGKNHWPQDIEWAVEQLPGFHQGDIAAFSVQADNGEEVP
                     AVLVHCRVSDPEERRRLHDQIKDKVRSITGMNCVVELVPPRTLPRTSSGKLSRAKAKK
                     LYLAGEIEPYKLSIAA"
     gene            2118..2678
                     /locus_tag="VN627_01130"
     CDS             2118..2678
                     /locus_tag="VN627_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HXD01885.1"
                     /translation="MSPSPRQKRTPRPLDAARLDELALAYVARFAASAAKLETYLQRK
                     LRERGWAGEGAPPVPAIVARFVAAGYVDDVAYARMKAGSLRRRGYGERRIGQALGAAG
                     IAEEVREEVRGAEGAQRRAALAFGRRKRLGPFGAGVDRAGQEKQLAAMLRAGHRMDLA
                     RAVVQAADMAALEQWAAEPEEEDDER"
BASE COUNT          451 a          840 c         1045 g          439 t
ORIGIN      
        1 cggctcgaac gaggcatgga acgacgcgtt tcgggcggtc ggcatcaagc ccgggatggc
       61 cggcgtgaag aagcagactg tccccggtgc ggctttggga gcgtcggcat cgccctcgcc
      121 gcggcgcggc agaccctgaa gcggcttagg ccgcgatcga cagcttgtag ggttcgatct
      181 cgccggcgag gtagagcttc ttggccttgg cccggctgag cttgcccgag ctggtgcgcg
      241 gcagggtgcg cggggggacc agttcgacca cgcagttcat gccggtgatc gagcggacct
      301 tgtccttgat ctgatcgtga aggcggcggc gttcttccgg gtcggagacg cggcagtgga
      361 cgaggacggc ggggacttcc tcgccattgt cggcctggac cgagaaggcg gcgatatcgc
      421 cctggtggaa gccggggagc tgttcgacag cccactcgat atcctgaggc cagtggttct
      481 tgccgttgat gatgatcatg tccttcgccc ggccgacgat gaacaggtag ccctcggcga
      541 ggtagcccat gtcgccggtg tcgagccagc catcgacgag gcaggcctcg gtcgattccg
      601 gatcgcggaa gtaggagtgc atgaccgagg ggcccttgca ccagaccttg ccgatgtggt
      661 gatcggccag cggcagcccg cgttcgccgc gcacttcgac gaccatgtcg cgcaccggct
      721 tgccgcagtt gacgatggcg cgatagcgcg cggggcgcga caggtcgcgc gggcagcccg
      781 agaggcgctc ttcctcgacc agttcgacgc ggatgccttc gcccgggggc atgatggtta
      841 ccgcgagagt cgcttcggcg aggccatagg aggggaggaa ggcggcggcc ttgaacccgg
      901 cctcggcaaa ggcgttgacg aagccctgca tgacgtcggg cctgatcatg tcggccccgt
      961 tgccggcgat ccgccagcgc gagaggtcga accgctcggc cacgtggctc tggctcgaga
     1021 tgcggcgggc gcagatgtcg tagccgaaag tgggggaata ggagagggtc gtgccgggat
     1081 tgcggctgat catgtcgagc caggccaggg gccggcgcgc gaaatcctcg gtcttgaggt
     1141 aatcggccga gacctggttg gcgatcggcg ataggaagca gccgacgagg cccatgtcgt
     1201 gataccacgg cagccacgag acgcagcggt cggtctggat cagcttcatc ccgtggccgt
     1261 ggccggcgag gttgttgagc agggcctcgt gggtgacggc gacgccatgg gggaagcggg
     1321 tcgaccccga ggaatactgc aggtagcaga tctggtcgga gctggccgcg ggcagggcca
     1381 cgccggcggg cgcgggatcg gcgaggaagt tctcgaaggc gatccccgcg cagccctgcc
     1441 gcgcggcggc ggcgccggcc atttcggcga tctcgccggg gaagaacagg atcgacgggt
     1501 cggagctgtt cagctgcacg acgagctgct cgatatagtt gtccttgccg ccgaacgagg
     1561 tcggcagcgg cagcggcacg ggccaggcgc cggcatagat cgtgccgcag aacagggcgg
     1621 cgaattcggg cccggtttcg gcgatgaggg cgatgcggtc ttccggcttg acgccctggg
     1681 ccaccaggcg gcgggcgacg gcgagggcat cctcgcgcag ttcggcgaag ggatagacgc
     1741 gggagaggtt gccgcgcgca tcgtggaaat taagcccgcg ggcacccttt gcggcatagt
     1801 ccagcgcatc gccgaaggtc gcgaaatcgg aaaagcggcg ctcaagcctg tcttcgttcg
     1861 gtgtcggcac cagcgtggca gtggtgtcgg tcatgcgcga atagcccgtc tgttgttgtt
     1921 gtcgccgtga ttgctgaagc tgcggattgt cgtcttgtga ttacgcgggg ctgaatgccc
     1981 tgatcgaaaa caagcactga tcggtaagca aatcccacca aaaccctgca ctggcgcaac
     2041 cccccggcca cgcaattggc cggtaattgc taccggcact ccccaatccg taacgactgt
     2101 ggcacgaata tggccgaatg tcaccgtctc cccgacagaa acgtacaccc cgccccctgg
     2161 atgccgcgcg cctggacgag ctggcgctcg cctatgtcgc ccgattcgcg gcgagcgcgg
     2221 ctaaactcga aacttacctt cagcgcaagc tcagggagcg cggctgggcc ggggaggggg
     2281 caccgccggt gccggcgatc gtcgcgcggt tcgtggctgc gggctatgtc gacgatgtcg
     2341 cctatgcccg gatgaaggcc ggatcgctgc gccggcgcgg ctatggcgag cggcggatcg
     2401 gccaggcgct gggcgcggcg ggcattgccg aggaggtgcg cgaggaggtg cgcggggcgg
     2461 aaggggcgca gcgccgcgcg gcgctggctt tcggcaggcg caagcggctg gggccgtttg
     2521 gcgcgggcgt tgaccgggcg gggcaggaaa agcagcttgc ggccatgctg cgggcagggc
     2581 accgcatgga ccttgcccgc gcggtcgttc aggcggccga catggcggca ctcgaacaat
     2641 gggccgccga gcccgaagag gaggatgacg agcggtgaag acgatcctgc gcaccctgct
     2701 gccgatcatg gcgctcgtat tggcggcccc gatgacgccc gcgctggcga ggccggctgc
     2761 ggcccattcc ggccc
//
LOCUS       DATNBV010000048         3441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_346467,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000048 DATNBV010000000
VERSION     DATNBV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346467"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VN627_01135"
     CDS             complement(<1..183)
                     /locus_tag="VN627_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HXD01886.1"
                     /translation="MVEQRIWTAALIVIGDEILSGRTHDKNIAQVAAWLGVQGIRLKE
                     VRVVADDTAAIVEAVNT"
     gene            246..1247
                     /locus_tag="VN627_01140"
     CDS             246..1247
                     /locus_tag="VN627_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006961548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HXD01887.1"
                     /translation="MSRPACQTGAMTIIAARSYRAGTTEEAALALDGCPRAAQPGLFD
                     WVGLCEPTPAEIELVRTQFGLHPLAVEDALNPTQLPKIDVYGPMLFIVARTAELEVGE
                     KIAYGQTALFLGPDYLVTVRFGSSRAHHPVRNRLEANGERLAEGGDYVAHAILDFIVD
                     GFVPILEKFEDAVQSMEERAIDSFPEPSTIRRIFRLRRQIRRFERIAGPMQEVCERLA
                     TTDLPNIDPAAHIWFRDVLDHVNRTLARMRGLKETLASIVETASLLEQHRQGEMTRQL
                     AAWAAILAVPTAIAGIYGMNFEFMPELHWRYGYFSAIALIVAVCVGLWWRFKRIGWL"
     gene            1265..2188
                     /locus_tag="VN627_01145"
     CDS             1265..2188
                     /locus_tag="VN627_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXD01888.1"
                     /translation="MAGGNTRTILIALGANVGIAAAKFAAAAITGSSAMLTEGVHSLV
                     DSTNQLLLLHGRRRAMRPADARHPFGYGRELYFWSFIVAILVFSLGAGVSVYEGVLHI
                     LEPEAASSPLVAFAVLAIAAALEGWSTIAALADFNKARGSRSVWSEIRKSKDTSNLVL
                     LLENAGALVGLGIAAVGLTISLVTGNPFWDGLASVVIGLVLGVLAVVLLYEAKGLLIG
                     ESADPRMVEAISRSAGAHPGVVKVHQVLTLHMAPEMVSAIITADFDDTISARAVEKIV
                     AAIQREVGAEFPIVTRVFVEPRDSRDPPPLD"
     gene            2237..>3441
                     /locus_tag="VN627_01150"
     CDS             2237..>3441
                     /locus_tag="VN627_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HXD01889.1"
                     /translation="MTDPAAHASLLRDGVLLLGFALGFVLVFRRLGLGATLGYLLAGA
                     VVGPQALHLVGDAEGKLGFAELGITLLLFIVGLELNPARLWKMRREIFGLGLLQVALC
                     GLAVAAFVTLGLGFTWAAAIALGLPLALSSTAQVLPMLQSSGRLRAPFGERAFAILLF
                     QDLSIIPLITIIAALSRNPAQQGGPPGWLLVLYTLGAIVGLVLAGRWLIRPLFRLIGN
                     MGEREMFVAAALFTVLAAGALMELLGLSTALGAFIAGVMLADSPYRHELEADVEPFRS
                     ILLGLFFLAVGMMLDLHAIAERPLFVAGVALALITIKSLVIFGIARLFGMAWRPALGL
                     GLLLSQGGEFGFVLFAQAQDALLIGPEAASLFGAVVTLSMATTPFLMKATARLRAEAE
                     AAPTGEREGP"
BASE COUNT          482 a         1296 c         1015 g          648 t
ORIGIN      
        1 ggtgttgacc gcctcgacga tcgccgccgt atcgtcggcc accacgcgca cttccttcag
       61 gcgaatgccc tgcaccccga gccaggccgc cacctgcgcg atgttcttgt catgcgtgcg
      121 gcccgaaagg atttcgtcgc cgatgacgat cagggcggcc gtccagatgc gttgctcaac
      181 catgcagggc tggctagccg ggtccgcgct gccgggcaag cgcggccctt cgggcaggcg
      241 ttgccatgag ccgcccggcc tgccagaccg gcgccatgac gatcatcgcc gcgcgcagct
      301 atcgtgccgg aactaccgag gaagccgccc tcgcgctcga cggctgcccg cgcgcagccc
      361 agcccggcct gtttgactgg gtcggcctct gcgaacccac gcctgctgaa attgagctcg
      421 tgcgcaccca gttcgggctc catccgctgg ccgtggaaga tgcgctcaat cccacccagc
      481 tgcccaagat cgatgtctac ggccccatgc tgttcatcgt cgcccgcacg gccgaactcg
      541 aagtgggcga aaagatcgcc tacgggcaga ccgccctgtt cctcgggccc gactatctcg
      601 taaccgttcg cttcggctcc agccgcgccc accatccggt ccgcaaccgg ctcgaagcca
      661 atggcgagcg gctggccgaa gggggagact atgtcgccca tgccatcctc gatttcatcg
      721 tcgatggctt cgtgcccatt ctcgaaaagt tcgaggacgc ggtccagtcg atggaggagc
      781 gggcgatcga ttccttcccc gagccctcca ccatccgccg cattttccgt ctgcgccgcc
      841 agatccgccg gttcgagcgc attgcaggcc cgatgcagga agtctgcgaa cgccttgcga
      901 cgaccgacct gcccaacatc gatcccgccg cccacatctg gtttcgcgac gtgctcgatc
      961 acgtcaaccg caccctggcg cggatgcgcg gcctcaagga aacccttgcg tccatcgtgg
     1021 aaaccgccag cctgctcgaa cagcaccgcc agggcgaaat gacccgccaa ctcgccgcct
     1081 gggccgcgat cctcgccgtc cccacggcca ttgccgggat ctacggcatg aacttcgaat
     1141 tcatgcccga actccactgg cgctacggct atttcagcgc gatcgccctg atcgtcgccg
     1201 tctgcgtcgg tctgtggtgg cgattcaagc ggatcggctg gctgtgatgg gagtttagaa
     1261 gcccgtggcc ggcggaaaca ccagaaccat tcttatcgcg ctcggcgcca acgtcggcat
     1321 cgccgcggcc aagttcgccg cagccgcgat caccggctcc tcggccatgt tgaccgaagg
     1381 tgtccactcc ctcgtcgatt cgaccaacca gctcctgctc ctccacggtc gccgccgcgc
     1441 catgcgcccg gccgatgcgc gccatccctt cggctatggg cgcgagctct atttctggtc
     1501 gtttatcgtc gcgatcctgg tcttctcgct cggcgcgggc gtctcggtct acgaaggcgt
     1561 attgcacatt ctggagcccg aggcggcctc gagcccgctc gtcgccttcg ccgtcctcgc
     1621 catcgccgcc gcgctcgaag gctggtcgac catcgccgcc cttgccgatt tcaacaaggc
     1681 acgcggcagc cgctcggtct ggtccgaaat ccgcaagtcg aaggacacct cgaacctcgt
     1741 cctcctgctg gaaaacgctg gtgccctggt cggcctcggc attgccgccg tgggcctcac
     1801 catcagcctc gtcaccggca atcccttctg ggacggcctt gcctcggttg tgatcggcct
     1861 cgtcctcggc gttctcgccg tggtcctgct ctatgaggca aaggggctgc tcatcggcga
     1921 aagcgccgac ccgcgcatgg tcgaagcgat ttcacgcagc gccggagcgc accccggcgt
     1981 ggtcaaggtc caccaggtcc tcaccctcca catggcgccc gagatggtct cggccatcat
     2041 caccgccgat ttcgacgaca cgatcagcgc gcgggcggtc gagaagatcg tcgccgcgat
     2101 ccagcgcgaa gtcggagccg agttcccgat cgtcacccgc gtcttcgtcg aaccccgcga
     2161 cagccgcgat ccgccaccgc tggattgacg gccccgtccg cattggcaat tccctgaaat
     2221 cccctagaca atccccatga ccgaccccgc cgcccacgcc tcgctcctgc gcgatggcgt
     2281 tctcctcctc ggctttgccc tgggcttcgt cctcgtcttc cgccgcctcg gccttggcgc
     2341 caccctcggc tatctgctcg ccggtgccgt cgtcggcccc caggcgctgc acctcgtcgg
     2401 cgatgccgaa ggcaagctcg gctttgccga actgggcatc accctgctgc tgttcattgt
     2461 cgggctcgag ctcaacccgg cgcggctgtg gaagatgcgg cgggaaatct tcggcctcgg
     2521 cctgctccag gtggcgctct gcggcctcgc cgtagccgcc ttcgtcaccc ttggcctcgg
     2581 ctttacctgg gccgcggcca ttgctctcgg cctcccgctg gcgctctctt ccaccgcgca
     2641 ggtcctgccc atgctgcaaa gctccggccg cctgcgcgcg ccgtttggcg agcgggcctt
     2701 cgccatcctg ctgttccagg acctgtcgat catcccgctg atcacgatca tcgccgcctt
     2761 gagccgcaac ccggcccagc agggcggccc gccgggctgg ctgctggtgc tttacacgct
     2821 gggcgccatc gtcggcctgg tccttgccgg gcgctggctg atccgcccgc tgttccgtct
     2881 gatcggcaac atgggcgagc gcgagatgtt cgtcgccgcg gccctgttta ccgtacttgc
     2941 cgccggcgcc ctgatggaac tgctcgggct ctctaccgca ctcggcgcct tcatcgccgg
     3001 ggtcatgctg gccgatagcc cctatcgcca cgagctcgaa gccgatgtcg agcccttccg
     3061 ttcgatcctg ctcggcctgt tcttcctcgc cgttggcatg atgctcgatc tccacgccat
     3121 tgccgaacgg ccgctgttcg tcgccggggt cgccctcgcc ctcattacga tcaagtcgct
     3181 ggtgatcttc ggcattgctc gcctgttcgg catggcctgg cgcccggcgc tcggcctcgg
     3241 cctcctgctc agccagggcg gtgaattcgg cttcgtcctc ttcgcccagg cgcaggacgc
     3301 cctgttgatc ggcccggaag cggccagcct gttcggagcg gttgtcactc tctccatggc
     3361 caccacgccg ttcctgatga aggccaccgc ccgcctgcgc gccgaggcgg aagccgcccc
     3421 cacgggcgaa cgcgaaggcc c
//
LOCUS       DATNBV010000049         4271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_994236,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000049 DATNBV010000000
VERSION     DATNBV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994236"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VN627_01155"
     CDS             <1..460
                     /locus_tag="VN627_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HXD01890.1"
                     /translation="GWSYGVRSAVSSPVGPRSFTVAAPVQADRTGDSIRLLLGDISAF
                     PAAKPVTPDELARVTDGNIRGLPNRYETNAQVLQAINTNQRLGRPDDYVTTLPARYRA
                     IDAAALDSAARQWLGPQGLIIVVVGDRKVVEPQLKGLALPVDYVTAPAGD"
     gene            515..814
                     /locus_tag="VN627_01160"
     CDS             515..814
                     /locus_tag="VN627_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01891.1"
                     /translation="MGVAGTYDVSVKSPMGELTGVLTVVPEGESFSGQLSGALGQVPI
                     SGGRIAGDTLTWQMGITSPMPLSLDCQATVSGDSLSGSVKAGAFGSMAIAGTRRA"
     gene            complement(852..1454)
                     /locus_tag="VN627_01165"
     CDS             complement(852..1454)
                     /locus_tag="VN627_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01892.1"
                     /translation="MTMKAVVRIALAAALVAVPASQAMAGWRLISAKQTVTVAKSKLA
                     VTPGEDWNRGTHRASAKGETWTIDGPSISEVYFAGGLQPGETFYKDRDKKNRPLPVMG
                     KNLQLTEVPEFYESSQRIAYDTAVYEITGIEPTKFMGQDGVKFTYTFALKDNPLKYKG
                     EARAALIKGALYLISFDAPELSFYERDRAKAEAIMDSARL"
     gene            complement(1451..2650)
                     /locus_tag="VN627_01170"
     CDS             complement(1451..2650)
                     /locus_tag="VN627_01170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXD01893.1"
                     /translation="MRLRDALMLLSALCVAAEPVMAQPGAVPPHAAAASASRDVWTEA
                     DETYVPQDQDERGLWMEMEKVERELKTSPLVIRDAALNAYVKSVLCKLIGPARCAPVR
                     LYIIRTSEFNATMAPNGMLEVYSGLLLRTRNEAQLAAVLGHEYTHYAKRHSLKLFRDV
                     RSKSASLMWFQMVVGGLLAGLLILPSIFKHSREMEQEADVGGLALMAAAGYDTREAAR
                     IWEQLRAEMDATALARKVKSKKDKDGGMFADHPPSAERVKYLTDAAVAQPGTPGVNGQ
                     DALAANLGGWWGAFADDQIKQNDFGASDYLITSLGKQGWTDWLHFARGELYRRHGAEG
                     DFDKAVGFYGEGIAAGASLPDLWRGRGLTLLKLGRTDEGRADLKEYLKRAPGAPDGAM
                     IAMMAGG"
     gene            complement(2662..3441)
                     /locus_tag="VN627_01175"
     CDS             complement(2662..3441)
                     /locus_tag="VN627_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01894.1"
                     /translation="MRRVFARAFGVCLALAAVQPAAAGVATKEGFAFPQGKEYRIIMF
                     RPDVQVGTLRTGGVDEANADWTATARANMQDAFQNAAEAREAQITFLGDPAGDSAKDL
                     NDFRGLFQVVSTEAMNHGLFADRLATKKILPTQPGGTTKLRFDWTLGADARKLKDITG
                     ADYAMFVYTHDAYGSAGRKMAQLMMAGLFGVAIVPGVHLGYAGMVDLNTGDLVWLNAD
                     IQMGGDLREKDGAEKRVKELLRGFPKRDAPPAAAIEDKASK"
     gene            complement(3557..>4271)
                     /gene="gatB"
                     /locus_tag="VN627_01180"
     CDS             complement(3557..>4271)
                     /gene="gatB"
                     /locus_tag="VN627_01180"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446471.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HXD01895.1"
                     /translation="RLFDPDTGTTRSMRSKEDAHDYRYFPDPDLLPLELDDAFVEACR
                     ASLPELPDAKRHRYENALGLTPYVAGVLTAEVETARWFEALLGEAAAKQGKGEEAVAK
                     QAANWLTSELFGALNRLGRSLDDSPLSPLQAAELLALIGNGTISGSIAKQVLEKMLET
                     GDGAQTIVEREGLRQESDTGAIEAVVAKVLADNADKVEQYKGGKEALFGFFVGQTMKA
                     MQGKANPQVVNELLKKALG"
BASE COUNT          689 a         1459 c         1369 g          754 t
ORIGIN      
        1 gggctggtcc tacggcgtcc gctcggccgt ctccagcccg gtcggccccc gcagtttcac
       61 cgtggcggcg ccggtccagg cggaccgcac gggcgattcg atccgcctgc tgctgggcga
      121 cattagcgcg tttccggcgg ccaagccggt gaccccagac gagcttgcgc gcgtgaccga
      181 cggcaacatc cgcggcctgc ccaaccgcta cgagaccaat gcgcaggtgc tgcaagcgat
      241 caacaccaac cagcgcctcg gccgcccgga cgactacgtc accaccctcc ccgcccgcta
      301 ccgcgccatc gacgccgccg ccctcgacag cgcggcgcgc cagtggctgg ggccccaggg
      361 gttgatcatc gtcgtggtgg gcgaccgcaa ggtcgtcgaa ccgcagctca agggcctcgc
      421 cctgccggtg gactatgtta ccgcgcccgc gggcgattga caccctcgcg cgcaaggccg
      481 accctcgcgc cgatcaaccg aaggagcaga cacgatgggc gtggcaggga cttacgacgt
      541 ttcggtcaag agcccgatgg gcgagctgac cggcgtgctg acggtggtgc ccgagggcga
      601 gagcttctcc ggccagctca gcggcgcgct gggccaggtg ccgatcagcg gcgggcgcat
      661 tgcgggagat accctcactt ggcagatggg gatcacctcg cccatgccgc tatcgctcga
      721 ttgccaggcg acggtcagcg gtgacagcct gagcggcagc gtgaaggccg gggccttcgg
      781 ctccatggcg atcgcgggga cacggcgggc atagcgcccg ccgcgtcccg aggcccgcgc
      841 cgcgggcggc gttagagccg cgcgctgtcc atgatcgcct cggccttggc ccggtcgcgt
      901 tcgtagaacg agagttcagg cgcatcgaag ctgatcaggt agagcgcgcc cttgatcagc
      961 gcggcacgag cctcaccctt gtatttcagt gggttgtcct tgagagcgaa ggtataggtg
     1021 aacttcaccc cgtcctggcc catgaacttc gtgggttcga tcccggtgat ctcgtaaacc
     1081 gccgtgtcgt aggcgatgcg ctgtgaactc tcgtagaact cgggcacctc ggtcagctgg
     1141 aggttcttgc ccatgaccgg gagcgggcgg ttcttcttgt ccctgtcctt gtagaatgtc
     1201 tcgcccggct ggagaccgcc cgcgaaatag acctcgctga tcgacgggcc gtcaatcgtc
     1261 caggtctccc ccttggcgct ggcgcggtgg gtgccgcgat tccagtcctc gcccggggtc
     1321 accgccagct tgctcttggc gacagtcacc gtctgcttgg cggaaatcag ccgccatccg
     1381 gccatagcct ggctggccgg cacggccacc agcgccgccg cgagcgcgat acgaaccaca
     1441 gccttcatcg tcatcctcct gccatcatgg caatcatcgc cccatcaggc gcacccggcg
     1501 cccgcttgag atattccttg agatcggccc tgccctcgtc ggtacggcca agcttgagca
     1561 aggtcagccc gcgcccgcgc cagagatcgg ggagcgaggc tcctgcggcg atgccttcgc
     1621 cgtagaagcc cacggccttg tcaaaatcgc cctcggcgcc gtggcggcga tagagttcgc
     1681 ctcgcgcgaa gtgcagccag tcggtccagc cctgcttgcc gagactggtg atcaggtagt
     1741 cgctcgcgcc gaaatcgttc tgcttgatct ggtcgtcggc gaaggcgccc caccacccgc
     1801 caaggttggc ggccaaggca tcctgcccgt tgacgcccgg ggtgcccggc tgggccaccg
     1861 ccgcgtcggt cagatacttc acccgctcgg ccgagggggg atgatcggcg aacatgccgc
     1921 cgtccttgtc cttcttcgac ttgaccttgc gcgccagcgc ggtcgcatcc atttccgcgc
     1981 gcagttgctc ccagatcctg gcagcctcgc gcgtgtcgta tccggcagcg gccatcaggg
     2041 cgagcccgcc gacatcggcc tcctgttcca tctcgcgcga gtgcttgaag atcgatggca
     2101 ggatcagcag tccggccagg agcccgccga ccaccatctg gaaccacatc aacgacgccg
     2161 atttggagcg gacatcgcgg aacagcttga gcgaatgccg cttggcgtaa tgggtatatt
     2221 catggccaag gacggccgcc aactgcgcct cgttgcgggt gcgcaggagc aggccggaat
     2281 agacctcgag catgccgttg ggcgccatgg tcgcgttgaa ttcggacgtg cggattatgt
     2341 agagccgcac cggagcacaa cgcgccgggc cgatcagctt gcacaagacc gatttcacat
     2401 aggcattgag cgccgcgtcg cggatcacga ggggcgaggt cttgagctca cgctcgacct
     2461 tttccatctc catccacaag ccgcgctcgt cctggtcctg cggcacgtag gtttcgtcgg
     2521 cctcggtcca tacgtctcgt gaagccgaag cggcggcggc atgcggcggg accgcgcccg
     2581 gctgggccat cacgggttcg gccgcgacgc agagcgccga gagcagcatc agggcatcgc
     2641 gcaggcgcac tgctttatcg cctacttcga cgccttgtcc tctatcgccg cggcgggcgg
     2701 cgcatcacgc ttcgggaagc cgcgcagcag ttccttgacg cgcttttccg cgccgtcctt
     2761 ttcgcgcaag tcgccgccca tctggatgtc ggcgttgagc cagacgagat cgccggtgtt
     2821 gagatcgacc atgcccgcat agcccaggtg cacccccggc acgatggcca cgccgaacag
     2881 gcccgccatc atcaactgcg ccatcttgcg gcccgcgctg ccataggcat catgggtata
     2941 gacgaacatg gcatagtccg cccccgtgat gtccttcagc ttgcgggcat cggcgccaag
     3001 ggtccagtcg aaccgcagct tggtggtgcc gcccggctgg gtcggcagga ttttcttggt
     3061 cgcgagccga tccgcgaaca ggccgtggtt catcgcctcg gtcgagacga cctggaacag
     3121 cccgcggaaa tcgttgagat ccttggcgct gtcgcccgcc ggatctccaa ggaaagtgat
     3181 ctgcgcctcg cgcgcctcgg cggcgttctg gaaggcgtcc tgcatgttgg cgcgggccgt
     3241 tgccgtccag tcggcattgg cctcatccac cccgccggtc cgcagggtgc ccacctgaac
     3301 gtcgggccgg aacatgatga tccggtattc cttgccctgc ggaaaggcga aaccctcctt
     3361 ggtggcaaca ccggcagcag ccggctgcac cgcagccagc gcaaggcaaa caccgaaagc
     3421 ccgcgcgaaa acgcggcgca tcgttgaaaa ctgcattgag atccccccac ccaatccgga
     3481 gtaaagtaga tttcagcagt ccactggcaa gagataattg ctcgagaagc gaacaattac
     3541 cgcttcgcac ttaggttcac cccagcgcct tcttcagcaa ctcgttgaca acctgcggat
     3601 ttgccttgcc ctgcatggcc ttcatcgtct ggccgacgaa aaacccgaac agggcttcct
     3661 tgccgccctt gtactgttcg accttgtcgg cattatcggc cagcaccttg gcgaccacgg
     3721 cttcgatcgc gccggtatcg ctttcttggc ggaggccttc gcgctcgacg attgtctggg
     3781 cgccgtcgcc ggtttccagc atcttttcca gcacctgctt ggcgatggag cccgagatcg
     3841 tgccgttgcc gatcagcgcg agcagttcgg cggcctggag cgggctgagc gggctgtcat
     3901 caagcgagcg gccaaggcgg ttgagcgcac cgaacagttc cgaggtcagc cagttggcgg
     3961 cctgcttggc cacggcttcc tcgcccttgc cctgcttggc cgcggcttcg cccagcaacg
     4021 cctcgaacca gcgcgccgtt tccacctcgg cggtcagcac gccggccacg tagggcgtaa
     4081 ggccgagcgc gttctcgtag cggtggcgct tggcatcggg cagctcgggc aggctcgccc
     4141 ggcaggcttc cacgaaggca tcgtccagct ccagcgggag caggtcgggg tcggggaagt
     4201 agcgatagtc gtgcgcgtct tccttcgagc gcatcgagcg ggtcgtcccg gtgtcgggat
     4261 cgaacaggcg g
//
LOCUS       DATNBV010000050         4616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1950818,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000050 DATNBV010000000
VERSION     DATNBV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950818"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /gene="dusB"
                     /locus_tag="VN627_01185"
     CDS             <1..745
                     /gene="dusB"
                     /locus_tag="VN627_01185"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HXD01896.1"
                     /translation="GEAAKLQEDRGAAIIDINMGCPVRKVVNGDAGSALMRDIPLATA
                     LIEATVKAVKVPVTVKMRMGWCHDSLNAPELARIAEDLGAKMITVHGRTRNQMYKGSA
                     DWAFVRKVKDAVSIPVIVNGDICGIEDAAKALEQSGADGVMIGRGAYGKPWLLAQVMH
                     WWKTGQGLPDPSIDLKYETLMEHYDAMLALYGRDTGVKMARKHLGWYTKGLPGSAEFR
                     NKVNFVDDPDEVVRSLKAFYAPFLTRQAA"
     gene            753..1871
                     /locus_tag="VN627_01190"
     CDS             753..1871
                     /locus_tag="VN627_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXD01897.1"
                     /translation="MAESPAPVPDAWRQLASLTVAVLLVGPERKIAGANPAAEQFLGQ
                     SLRRISGRRLFELIRFDEPRLMDRAGDTDSPLSARDAWATVAGVGRRRLDVTIGPVID
                     APEWQVVTFQDASAAEALGEDPGSSDDSVLRAPEILAHEIKNPLAGIRGAAQLLARKV
                     GEKDRALTSLIADEVDRIAKLIDEMQTLSRRTAAPVAPCNLHEAVRRARAVIDAAGKG
                     PRIEEEFDPSLPPVLGSADALVQVLINLLSNAAEACRGRPEPRIVVRTRFSSGLQLHA
                     VSDGKPVRLPIELRVSDNGPGVDPAMRDHIFEPFVTTKKSGQGLGLALVRKLVRDMNG
                     RITHEREDDSRTGGGWTHFRIHLPLAQEPRRSSRGKAA"
     gene            1868..3286
                     /locus_tag="VN627_01195"
     CDS             1868..3286
                     /locus_tag="VN627_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXD01898.1"
                     /translation="MSVLLVEDDTSIAIVITAALEAEGFDVTCCDSVADRDRLLSERA
                     YDALVTDVMLTDGDGIESLPAVRALHPDMPVIILSAQNTLDTAVRASDTGAFEYFPKP
                     FDIDELARTVRQAVGNRAGATQARPDDASTEGLPLVGRSAPMQAVFRMITRVLRNDLT
                     VLILGESGTGKELVAEAIHQLGHRRAGPFVAVNTAAIPADLIESELFGHEKGAFTGAV
                     ARHIGKFEQAAGGTLFLDEIGDMPMQAQTRLLRALQSGSIRRVGGREEIAIDTRIVAA
                     TNKDLEPMIAAGLFREDLFYRLNVVPIHMPALRERSDDIEALANHFLKLAALEGLPRR
                     QLTRDACELLARQPWRGNVRELKNFIYRLALLAREDLVDAASLQPLLVSTQRAEEAAR
                     GDEVPLDMAVTRWLAREAPTPGRVYDEALAAFERPLFAEVLRETGGNQLRAAQILGIN
                     RNTLRKRLSELELDPEGFGRRG"
     gene            3426..>4616
                     /locus_tag="VN627_01200"
     CDS             3426..>4616
                     /locus_tag="VN627_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HXD01899.1"
                     /translation="MAVWARRANIYALIEMIAAFAFVSMTLVTFATIRAQSDRELMPS
                     DLTATLLVGTLVPAMALLVLLGRRMALRRAAQTLGGTGRLHTQLVFFFSLISAIPTLL
                     VVVFASYLFQSGVEFWFSDSSRGMLENANKLARGYYEQNLRDVGNETIAMATDLRDYL
                     SQTRITSPQFAEGYSYQVVSRKLNESAIIQKAPDGKLLTAAIVDPEKNSTRERITADV
                     LGRLEKGEPVVVSASANRIDAVAPIDRETGVYLYAARSSDLLAFSQGQRAQNIVRAYE
                     VLTRRARTLQLRFNVALFVASLALVGLSVWFALRFADRQVKPLYDLVGAARRVGSGNY
                     ALRVEGRTGPDEIGQLNRAFNRMTAQIERQTQALVGANQQLHERRAFIEAVLESVSAG
                     VISAD"
BASE COUNT          762 a         1511 c         1562 g          781 t
ORIGIN      
        1 gggcgaggcg gcaaaactgc aggaagaccg cggcgccgcg attatcgaca tcaacatggg
       61 ctgcccggtg cgcaaggtcg tcaacggcga tgccggctcg gccctgatgc gtgacatccc
      121 gctggcaact gccctgatcg aggctacggt gaaggcggtg aaggttccgg tcacggtcaa
      181 gatgcggatg ggctggtgcc atgacagcct gaacgcgccc gagctggcgc ggattgcgga
      241 agatctcggg gcaaagatga tcactgtgca cggccgcacc cgcaaccaga tgtacaaggg
      301 ttctgccgac tgggctttcg tccgcaaggt caaggacgcg gtttccattc ccgtcatcgt
      361 caacggtgac atctgcggga tcgaggatgc cgcgaaagcc cttgaacaat ccggcgcgga
      421 tggggtgatg atcggtcggg gcgcctatgg caagccgtgg ctgctcgccc aggtgatgca
      481 ctggtggaag accgggcagg ggctccccga tccgtcgatc gacctgaagt acgagacgct
      541 gatggagcac tatgacgcca tgcttgccct ctacgggcgg gataccggcg tgaagatggc
      601 gcgcaagcac ctcggctggt acaccaaggg cctgcccgga tcggcggaat tccgcaacaa
      661 ggtcaacttc gttgacgatc cggacgaggt ggtgcggagc ctcaaggcgt tctacgcccc
      721 gttcctgacg cggcaggcgg cctgacgcgg ccatggccga gagcccggcc ccggtccccg
      781 atgcttggcg ccagctggcc agcctgacgg ttgccgtcct gctggtcggc cctgagcgca
      841 agatcgccgg ggccaatccg gcagccgagc agtttcttgg gcagagcctc aggcggatca
      901 gcggccggcg cctgttcgag ctgatccggt tcgacgaacc ccgcctgatg gaccgcgccg
      961 gcgacaccga cagcccgctt tccgcccgcg atgcctgggc cactgtcgcg ggcgttggcc
     1021 ggcgtcggct cgacgtgacg atcgggccgg tgatcgacgc tccggagtgg caggtcgtca
     1081 ccttccagga tgcgagcgcg gctgaagccc tcggcgaaga tccgggaagt tcggacgatt
     1141 ccgtcctgcg cgcacccgag attctcgctc atgagatcaa gaacccgctc gccggcatac
     1201 gcggcgcggc gcagttgctc gcccgcaagg ttggcgagaa ggaccgcgcg ctgaccagcc
     1261 ttatcgccga cgaggtcgac cggatcgcca agctgatcga cgagatgcag accctctcgc
     1321 gccgcactgc cgcgccggtc gccccgtgca acctccatga ggcggtgcgc cgcgcccgcg
     1381 ccgtgatcga cgctgcgggc aaggggccgc gcatcgagga agagttcgac ccctcgcttc
     1441 caccggtgct gggcagcgcc gatgcgctgg tccaggtgct gatcaacctg ctgtcgaatg
     1501 cggccgaggc ctgtcgcggt cgtcccgagc cgcgcattgt cgtgcgcacc cgcttttcca
     1561 gcgggctcca gctccatgcg gtgagcgacg gcaagccggt gcgtctgccc atcgaactgc
     1621 gtgtgtcgga taacggaccg ggtgtcgacc ccgccatgcg cgatcatatt ttcgagccct
     1681 tcgtcaccac caagaagagc ggtcaggggc tgggcctcgc gctcgtgcgc aagctggttc
     1741 gcgacatgaa cgggcgcatc acccatgagc gcgaggacga cagccggacc ggtggcggct
     1801 ggacccactt ccgcatccac ctcccgctgg ctcaggagcc gcgccgatca tcgaggggca
     1861 aggcagcatg agcgttctcc tcgttgaaga cgacacctcg atcgccatcg tcatcactgc
     1921 cgcgctggag gccgagggct tcgacgtgac ctgctgcgat tcggtggctg atcgcgaccg
     1981 gctcctgtcg gagcgcgcct atgatgcgct ggttaccgat gtcatgctga ccgatggcga
     2041 cgggatcgaa tccctgcctg ccgttcgcgc gcttcatccc gacatgccgg tgatcatcct
     2101 gtccgcgcag aacacgctgg atacggcggt tcgcgccagc gataccgggg ccttcgagta
     2161 tttccccaag cccttcgaca tcgacgaact ggcgcgcacc gtgcggcagg ccgtcgggaa
     2221 cagggccggc gccacgcagg cgcgcccgga tgacgcatcg accgaagggc tgcccctcgt
     2281 gggccgtagc gcgccgatgc aggccgtgtt ccggatgatc acgcgggtgc tgcgcaacga
     2341 cctgaccgtg ctgatcctgg gggaaagcgg gactggcaag gaactggtgg cggaagcgat
     2401 tcaccagctg ggtcaccgcc gcgccggacc gttcgtagcg gtgaacacgg cggcgattcc
     2461 cgccgacctg atcgagagcg aactgttcgg ccacgagaag ggcgccttca ccggggcggt
     2521 agcccggcac atcggcaagt ttgagcaggc cgccggcggc accctgttcc tcgatgagat
     2581 cggcgatatg ccgatgcagg cccagacccg gctgctgcgg gcgctgcaat cgggctcgat
     2641 ccggcgagtg ggcgggcgcg aggagatcgc gatcgacacc cgcatcgtcg ctgcgacgaa
     2701 caaggacctc gagccgatga tcgccgcggg cctgtttcgc gaggacctgt tctaccggct
     2761 caatgtcgtg ccgatccaca tgcccgccct gcgcgagcgt tccgacgaca tcgaggcgct
     2821 ggccaatcat ttcctcaagc ttgccgcgct ggagggcctg ccgcgccggc agttgacgcg
     2881 tgacgcctgc gagctgctcg cccggcagcc gtggcgtggc aatgtgcgcg aattgaagaa
     2941 cttcatctac cggctcgccc tgctcgctcg cgaagacctg gtcgatgccg cttcgctcca
     3001 gccgctgctg gtttcgaccc agcgcgcgga agaagccgcc cggggtgacg aggtgccact
     3061 agacatggcc gtaacgcggt ggctggcgcg cgaggcgccc acgcccgggc gggtctatga
     3121 cgaagcgctt gccgccttcg agcggcccct gtttgccgag gtcctgcgtg agaccggggg
     3181 caaccagctg cgcgcggcgc agatcctcgg gatcaatcgc aacacgctgc gcaaacggct
     3241 gtcggagctg gaactggacc ctgaaggctt cgggcggcga gggtagcggg ttacgccgaa
     3301 aatcgcttgc aatgcctcga tctgggtgtt gtaaccgcgc aactatgacg cagccgcctg
     3361 tatcagtcat gccacagctg gccgcaccgg cgcggtctgg ccggtggccg cgctggtggc
     3421 ggcggatggc ggtctgggca cgccgggcaa atatctatgc cctgatcgaa atgatcgcgg
     3481 ccttcgcctt cgtctcgatg actttggtga ccttcgccac gatccgggcc cagtcggatc
     3541 gcgaactgat gccgtcggac ctgaccgcga ccctgctggt gggcaccctg gttccggcca
     3601 tggccctgct ggtcctgctc gggcggcgca tggcactgcg ccgcgcggcg caaacgctgg
     3661 gcggtaccgg gcgactgcac acccagctgg tgttcttctt ctcgctgatt tcggcaatcc
     3721 cgaccctgct ggtggtggtt ttcgcgtctt acctgttcca atcgggcgtg gagttctggt
     3781 tttctgactc gtcgcgcggc atgctggaga acgccaacaa gctggcgcgc ggttattacg
     3841 agcaaaatct gcgcgacgtc ggcaacgaga ccatcgccat ggcgacggac ttgcgcgact
     3901 atctgtcgca gacccgcatc accagcccgc agttcgccga gggctattcg tatcaggtcg
     3961 tcagccgcaa gctcaatgag agcgcgatca tccagaaggc cccagacggc aagctgttga
     4021 cggcggccat cgtggatccg gaaaagaact caacccgcga gcggatcacg gccgacgtgc
     4081 tcggccggct ggaaaagggc gagccggtgg tcgtctcggc cagtgccaac cgcatcgacg
     4141 ccgtcgctcc gatcgaccgg gaaaccgggg tctatctcta tgccgcccgc tcgtctgacc
     4201 tgctggcctt cagccaggga cagcgcgccc agaacattgt tcgtgcctat gaagtgctga
     4261 cgcggcgcgc gcggacgttg cagctgcggt tcaacgtggc cctgttcgtc gccagcctgg
     4321 cgctggttgg cctgtcggtc tggtttgcgc tgcgatttgc cgaccggcaa gtgaagccgc
     4381 tttacgacct tgtcggcgcc gcccggcggg tgggcagcgg caactatgcc ctgcgcgtgg
     4441 agggacggac ggggccggac gagattggcc agctcaatcg cgccttcaac cgaatgaccg
     4501 cccagatcga gcgccagacc caggctctgg tcggtgcgaa ccagcagttg catgaacgcc
     4561 gcgccttcat tgaggcggtt ctggaatcgg tcagcgcagg cgtcatttcc gcggac
//
LOCUS       DATNBV010000051         6425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1951215,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000051 DATNBV010000000
VERSION     DATNBV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951215"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VN627_01205"
     CDS             complement(<1..414)
                     /locus_tag="VN627_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXD01900.1"
                     /translation="MDIPGLPARRAALKLLDAVLRRGETLDQAGHNAVQGLAADADKA
                     LARAIAAEALRWLPDFDALIDSATRQPLPDDAKPRSVLRLMLAQWLRLQTPPHAVVAT
                     ALPLLAGGPKRLAHGVFATLTRRGATLPRHPTLPAA"
     gene            469..636
                     /locus_tag="VN627_01210"
     CDS             469..636
                     /locus_tag="VN627_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HXD01901.1"
                     /translation="MTERATKRPDGFVKPAHWTTEPAPQPAPAKDEPDPQGLSPTRYG
                     DWEKNGIAVDF"
     gene            complement(735..1181)
                     /locus_tag="VN627_01215"
     CDS             complement(735..1181)
                     /locus_tag="VN627_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HXD01902.1"
                     /translation="MTPDVRLALAPFDPAAELAAFAALHPGAGGIVTFLGQVRAGDGV
                     EALELRHYGPLTLPGMTELAGTVAARWMLDGLLILHRSGEMQPGEPIVLVAAASRHRR
                     DAFAAADFAMDHLKSDSWFWKREKVAGAWRWIEPREQDHADLARWG"
     gene            complement(1186..1428)
                     /locus_tag="VN627_01220"
     CDS             complement(1186..1428)
                     /locus_tag="VN627_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXD01903.1"
                     /translation="MARLVFLGRLEDVAGVPHMDCPLAEPTALADLLLMLPPQLAEAL
                     QGPRVRIAHNGVVTEAAGLVVGDGDELAFLPPVSGG"
     gene            complement(1435..2436)
                     /gene="moaA"
                     /locus_tag="VN627_01225"
     CDS             complement(1435..2436)
                     /gene="moaA"
                     /locus_tag="VN627_01225"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HXD01904.1"
                     /translation="MSAAPLVDQFQRRITYLRLSVIDRCDLRCAYCMPERMTFLPKAE
                     VLTLEELHQLALGFIARGVTKLRLTGGEPLVRRDMIELVRALGRKLGDGLDELTLTTN
                     GTRLAEFAEGIAAAGVRRINVSLDTRSREGFASLARRDALPQVLEGIAAARAAGLKVK
                     LNTVALKGINEDELPDLIAWAHGQQMDLTLIEVMPLGEVEEDRFDHYLPLSAVRERLE
                     RDWTLTPSPHRTGGPARYFDIAETGGRLGLITPLTNNFCDGCNRVRVTATGQLFACLG
                     GTEQVDLRAALRSADPERELEAALDLAMKIKPKRHAFAIDKPGAAPALPRHMSMTGG"
     gene            complement(2433..2774)
                     /locus_tag="VN627_01230"
     CDS             complement(2433..2774)
                     /locus_tag="VN627_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01905.1"
                     /translation="MQLAVDALPEDPIAAAARFHAEWLPRILAALDAGEGLVTLVFPP
                     AGLAHAGWREAAVQLLARERTPARINAIVGEDPAGIAATAQFIAAAPGLTGQYLVLDG
                     NGAGPVLYNGS"
     gene            complement(2783..3154)
                     /locus_tag="VN627_01235"
     CDS             complement(2783..3154)
                     /locus_tag="VN627_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HXD01906.1"
                     /translation="MADSLILEVNDLEVDVLTGIYSEETGKPQPLRISIAARLKPAPH
                     FAADTPLSASKNYMDLKFAAGEGLPPGVHFKLIEAVADHICETVMLGDPRVEAVTVKI
                     VKLAIAEAGEQIGMTLHRDRR"
     gene            complement(3250..4134)
                     /locus_tag="VN627_01240"
     CDS             complement(3250..4134)
                     /locus_tag="VN627_01240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXD01907.1"
                     /translation="MAELVQQPRLLVGEGWADYGLVDSGHGRKLERYGPYRFIRPEPQ
                     ALWTPREADWQAHGEFVPGSDEDGGGRWQFMQSVPREGWPLARGEVTFTASCTPFRHL
                     GFFPDMAPVWDWMGEMLALKAEASTLNLFGYTGVGTLSLSAHGPVTHVDASKKSVAQA
                     RANAALSGMEQRPVRWIVDDAAKFAAREVRRGRRYDGIILDPPKFGRGPEGEVWRLEE
                     HLPGLVADCRRLLDADSRFLFLTVYAVRMSSLALAGLLDEVFAGLPGRIEHGDLAVRE
                     DGAGRLLPTAIFARWAND"
     gene            complement(4134..5534)
                     /gene="thrC"
                     /locus_tag="VN627_01245"
     CDS             complement(4134..5534)
                     /gene="thrC"
                     /locus_tag="VN627_01245"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXD01908.1"
                     /translation="MQYISTRGSAPALDFAGATLAGLASDGGLYVPGEWPRFSEAEIA
                     AMAGLPYAELAQKIMQPFVGDCLTPERLLELTRQAYGRFSHKAVTPLVQLDEQQWLLE
                     LFHGPTLAFKDVALQLLGLLFEEFLSRSDRNLTIVGATSGDTGSAAIDAVAGRAKIDI
                     FMLHPKGRVSDVQRKQMTTVLAPNVHNIAIEGSFDDAQAMVKRMFNDAAMTDRFAIGA
                     VNSINWARLMAQVVYYFAAALQLGAPARKVAFSVPTGNFGDVFAGYVAAKMGLPIERL
                     IVATNVNDILHRALANGDYSQGTVTPTAAPSMDIQVSSNFERLLFDVGGRDGVALAAQ
                     MKGFEASKAMQLTNAQREGASALFSSCRADADEMTAAIRWAFHECGETIDPHTAIGLH
                     AARVAGIDPAVPVVTLATAHPAKFTEAVTAATGLHPALPARVGDLFEREERLVDLPGD
                     YAAVADYVAAHASPKA"
     gene            complement(5621..>6425)
                     /locus_tag="VN627_01250"
     CDS             complement(5621..>6425)
                     /locus_tag="VN627_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXD01909.1"
                     /translation="AGMRECGVAEGFFFLIRTSILKALYPLGPDRVYGWNVDVTTCRE
                     VYRQERIVVADDRIMIHHPDRRPDHAIDDDAAVDEAIRHGALELKEWLVAMRERCLRP
                     APTINPTAALDLGCGPHPQNNFGANALFGIDIREIDNPNIKRADLNIEPIPFADNAFD
                     YVTASDFVEHVARLLYAPTQRFPFVELMNEIHRVLKPGGVFLSITPAYPDVKAFQDPT
                     HVNFITENTFADYFCVPNLWAAMYGYSGRFRLLHQAWDDGKLITFLRAI"
BASE COUNT         1064 a         2285 c         2134 g          942 t
ORIGIN      
        1 cgccgccggc agggtcggat gccgcggcag agtcgcgccc cgccgggtca gcgtggcaaa
       61 caccccatgc gccagccgct tgggcccacc cgccaacagc ggcagcgccg ttgccaccac
      121 ggcatggggc ggcgtctgca gccgcagcca ttgcgccagc atcagccgca gcaccgatcg
      181 gggcttggca tcgtcgggca gcggctggcg ggtggcgctg tcgatcagcg cgtcgaagtc
      241 cggcaaccag cgcagcgcct cggcagcaat cgcgcgggcg agcgccttgt cggcgtcggc
      301 agcgaggccc tgcaccgcat tgtgcccggc ctggtcgagc gtttcgcccc gccgcagcac
      361 ggcatcgagc agtttgagcg cagcgcggcg cgcgggcagg ccgggaatgt ccatggacgg
      421 cccctagagg cgattgcaat ccggcgccag cgcgcccatg tcgctcgcat gacagagcgc
      481 gccaccaaac gcccggacgg cttcgtcaag cccgcccact ggaccaccga acccgcgccc
      541 cagccggcgc cggccaagga cgaacctgat ccccagggcc tgtcgcccac gcgctacggc
      601 gattgggaaa agaacggcat cgccgtcgat ttctgagcgg ctaccgcttg atcactcccc
      661 gccggggaaa gatacgcagg ctgagagcga agcgaactag ccgaagttga gatgggcctc
      721 ggccagatcc agcctcaccc ccaccgggcc aggtcggcgt gatcctgctc gcgcggctcg
      781 atccagcgcc atgcgccggc aaccttttcg cgcttccaga accacgaatc gctcttgagg
      841 tgatccatgg cgaaatccgc cgcggcaaag gcatcgcggc ggtgccggct ggccgcggca
      901 accagcacga tcggttcgcc cggctgcatc tcccccgaac ggtgcaggat cagcagcccg
      961 tccagcatcc accgtgccgc caccgtcccg gccagttcgg tcatgcccgg cagggtcagc
     1021 ggcccgtagt gccgcagctc cagcgcctcc accccatcgc ccgcgcggac ctgccccaga
     1081 aacgtcacga tccccccggc gcccggatga agcgcggcaa acgccgccag ctcggctgcc
     1141 ggatcgaacg gagccagcgc aaggcgaacg tccggcgtca tcgcctcagc cgccgctgac
     1201 cggcggcagg aaggccagct cgtcgccgtc cccaaccacc agcccggccg cttcggtcac
     1261 cacgccgttg tgggcaatcc gcacccgtgg cccttgcagc gcctcggcca gctgcggcgg
     1321 gagcatgagc aggagatcgg ccagcgccgt cggctcggcc agcgggcagt ccatgtgggg
     1381 gacgccggca acgtcttcca gccgcccgag gaacaccagc ctcgccaccc gaaatcagcc
     1441 gccggtcatc gacatatggc gcggcagcgc cggcgccgcg ccgggcttgt cgatggcgaa
     1501 ggcatggcgc ttgggcttga tcttcatcgc caagtcgagc gccgcttcca gttcccgttc
     1561 cggatcggcc gagcgcagcg ccgcgcgcag gtcgacctgc tcggtcccgc caaggcaggc
     1621 aaacagctgg cccgtcgcgg tgacccgcac ccggttgcag ccgtcgcaga aattgttggt
     1681 gagcggtgtg atcaggccca gccgcccgcc ggtctcggcg atgtcgaagt agcgcgccgg
     1741 cccgcccgtc cgatggggcg aaggggtcag cgtccagtcc cgctccagcc gctcgcgcac
     1801 ggcggacagc ggcaggtaat ggtcgaagcg gtcttcctcg acctcgccca gcggcatgac
     1861 ctcgatcagg gtcaggtcca tctgctggcc gtgggcccaa gcgataaggt cgggcagttc
     1921 gtcttcgttg atgcccttga gcgccaccgt gttgagcttg accttgagcc cggccgcccg
     1981 cgcggcggca attccctcca gcacctgcgg cagagcatcg cgccgggcca ggctggcaaa
     2041 gccctcgcgg ctgcgcgtat caagcgagac gttgatccgg cgcacgcccg ccgccgcgat
     2101 gccctcggcg aattcggcca ggcgggtgcc gttggtggtg agggtcagct cgtccagccc
     2161 atctccgagc ttgcggccaa gcgcgcggac cagctcgatc atgtcgcgcc gcaccagcgg
     2221 ctcgcccccg gtcaggcgca gcttggtcac gccccgggcg atgaagccca gcgccagctg
     2281 gtgcagttct tccagcgtga gcacttccgc cttgggtagg aaggtcatcc gctcgggcat
     2341 gcaataggcg cagcgcagat cgcaccggtc gatcaccgac aggcgcaagt aggtgatccg
     2401 gcgctggaac tggtcgacaa ggggggcggc gctcatgagc cgttatatag caccgggcca
     2461 gcgccattgc catcgagcac cagatattgt ccggtcagcc cgggcgcggc ggcgatgaac
     2521 tgcgccgtgg cggcgattcc ggccgggtcc tcgcccacga tggcgttgat ccgcgccggg
     2581 gtccgctcgc gggccagcaa ctgcaccgcc gcctcgcgcc agccggcatg ggccagtccc
     2641 gcgggcggga agaccagcgt caccagcccc tcgcccgcat cgagcgcggc gaggatccgg
     2701 ggcagccact cggcgtggaa ccgcgccgcc gcggcgatcg ggtcctccgg cagggcatcc
     2761 accgccagct gcatggccgc cgtcagcgcc ggtcgcggtg aagggtcatg ccgatctgct
     2821 cgccggcctc ggctatggcg agcttgacga tcttgaccgt caccgcctcg acgcgcggat
     2881 cgcccagcat cacggtttcg caaatgtggt cggccacggc ctcgatcagc ttgaagtgca
     2941 cccccggcgg caggccttcg cccgcggcga acttgagatc catgtagttc ttgctggccg
     3001 aaaggggcgt gtcggcggca aaatgcggcg ccggcttcag ccgagccgcg atcgagatgc
     3061 gcagcggctg cggcttgccg gtctcttccg aatagatgcc ggtgaggaca tcgacttcga
     3121 gatcgttcac ttcaaggatg aggctgtcgg ccatggcacg cggcgatggc atggtgccgg
     3181 gccgctgtcc acccccggcc gcccgggcca tcaagacggc ctgcgggccc ggcgcagcgg
     3241 gcaatcaggt cagtcgttcg cccaccgcgc gaagatcgcc gtcggcagca gccgccccgc
     3301 cccgtcctcg cgcacggcaa ggtcaccatg ttcgatccgc ccgggcagac cggcgaacac
     3361 ctcgtcgagc aggcccgcca gcgccagcga gctcatccgc acggcataaa ccgtcaggaa
     3421 caggaagcgg ctatcggcat cgagcaggcg ccggcagtcg gccaccaggc ccggcaggtg
     3481 ctcctccagc cgccacacct cgccttccgg cccacgcccg aacttgggcg ggtcgaggat
     3541 gatcccgtca tagcgccgcc cccgccgcac ctcgcgcgcg gcaaacttgg ccgcatcgtc
     3601 gacgatccag cgcaccgggc gctgctccat gcccgacagg gcggcattgg cgcgggcctg
     3661 ggcgaccgac ttcttgctcg catcgacatg ggtaaccggc ccatgcgccg agagcgaaag
     3721 cgtgccgacc ccggtatagc caaacaggtt gagcgtgctc gcctcggcct tcagtgccag
     3781 catctccccc atccagtccc acaccggcgc catgtccgga aagaagccaa ggtgccgaaa
     3841 cggcgtgcag cttgcggtga acgtcacctc gccccgtgcc agcggccacc cctcgcgcgg
     3901 cacggactgc atgaactgcc acctgccccc gccatcctcg tccgaaccgg gcacgaattc
     3961 gccatgcgcc tgccagtccg cctcgcgcgg ggtccacagg gcctgcggtt cggggcggat
     4021 gaagcgatag ggcccgtagc gttcaagctt gcggccatgg ccggaatcga ccagcccata
     4081 gtcggcccag ccctcgccca cgagcaggcg gggttgctgg accagctcgg ccatcaggct
     4141 ttcgggctgg cgtgagccgc cacgtagtcg gccacggcgg catagtcgcc gggcaggtcg
     4201 accagccgct cctcgcgctc gaacaggtcg cccacgcgcg ccggcagcgc cgggtgcagg
     4261 cccgtcgccg cggtcaccgc ctcggtaaac ttggccggat gcgccgtggc cagcgtgacg
     4321 acaggcaccg ccggatcgat ccccgccacg cgggccgcat gaaggccgat cgccgtgtgc
     4381 ggatcgatcg tttcgccaca ctcatggaac gcccagcgga tcgccgcggt catctcgtcc
     4441 gcatcggccc ggcacgagga aaacagcgcc gaggccccct cgcgctgggc attggtgagc
     4501 tgcatcgcct tgctcgcctc gaagcccttc atctgcgcgg ccagcgccac accgtcgcgc
     4561 ccgccgacat cgaacagcag ccgctcgaag tttgacgaaa cctggatgtc catcgacggc
     4621 gccgcggtcg gcgtcaccgt tccttgcgaa taatcgccat tggccagcgc gcggtggagg
     4681 atgtcgttga cattggtcgc cacgatcagc cgctcgatcg gcagccccat cttcgctgcg
     4741 acatagccgg caaacacgtc accgaaattg cccgtcggca ccgagaaggc caccttgcgg
     4801 gccggggcgc ccagctgcag cgcggcggcg aaatagtaga ccacctgggc catcagccgc
     4861 gcccagttga tcgaattcac cgcgccgatc gcgaagcggt cggtcatggc cgcgtcgttg
     4921 aacatccgct tcaccatggc ctgggcatcg tcgaacgagc cttcgatggc gatgttgtgg
     4981 acgttgggcg cgagcacggt ggtcatctgc ttgcgctgca cgtcggagac gcggcccttg
     5041 gggtgcagca tgaagatgtc gatcttcgcc cgcccggcca cggcatcgat ggccgccgag
     5101 ccggtgtcgc ccgaggtcgc gccgacgatg gtcaggttcc ggtccgagcg cgagaggaac
     5161 tcctcgaaca gcagtccgag cagctgcagc gccacgtcct tgaaggccag cgtcggtccg
     5221 tgaaacagtt caagcagcca ctgctgctcg tccagctgaa ccagcggcgt caccgccttg
     5281 tggctgaacc ggccataggc ctgacgggtc agttccagca gccgctccgg cgtcaggcag
     5341 tcgcccacga agggctgcat gatcttctgc gccagctcgg catagggcag gccggccatg
     5401 gccgcgatct cggcttccga gaagcgcggc cattcgccgg gcacatagag cccgccgtca
     5461 gaggcaaggc cagcaagagt ggcgccggca aaatcgagcg caggggcgga gccgcgggtg
     5521 ctgatatact gcatcaagcg ccgttagcct cgcccgcgca gaatgaaaac ccttggccgc
     5581 tggcgcggcg tagatagcga ccgtccggac gcgaccgggc tcagatcgcg cgcaggaagg
     5641 tgatcagctt gccgtcgtcc cacgcctggt gcagcaagcg gaaccggccg gaatatccgt
     5701 acatggccgc ccacaggttg ggcacgcaga aatagtctgc gaaggtgttt tccgtgatga
     5761 aattcacatg ggtcggatcc tggaacgcct tcacgtcggg ataggccggc gtgatcgaca
     5821 ggaacacgcc gccgggcttc agcacccgat ggatctcgtt catcagctcc acgaagggaa
     5881 aacgctgggt gggcgcataa agcagccgcg cgacatgctc gacgaagtcg ctggcggtga
     5941 catagtcgaa ggcattgtcc gcgaagggga tcggctcgat gttcaggtcc gcccgcttga
     6001 tgttcgggtt gtcgatctcg cggatgtcga tgccgaacag cgcgtttgcg ccgaaattgt
     6061 tctggggatg cgggccgcag ccgaggtcga gcgccgcggt tgggttgatc gtgggggccg
     6121 gccgcaggca tcgctcccgc atcgcaacga gccattcctt caactccagc gcaccgtggc
     6181 ggatggcttc gtccacggct gcgtcgtcgt cgatggcatg gtccggccgg cgatccggat
     6241 ggtggatcat gatgcggtcg tcggcgacaa cgatgcgctc ctgccgatag acctcgcggc
     6301 acgtggtgac atccacgttc cagccataga cccggtctgg gccgagcgga taaagcgcct
     6361 tgaggatgct ggtacggatc aggaagaaaa agccctcggc cacgccgcac tcgcgcatgc
     6421 ccgcc
//
LOCUS       DATNBV010000052         3312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_48146,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000052 DATNBV010000000
VERSION     DATNBV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48146"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1085)
                     /locus_tag="VN627_01255"
     CDS             complement(126..1085)
                     /locus_tag="VN627_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXD01910.1"
                     /translation="MDLSIRRIKQFIAVAEEQHFGRAALRLNMAQPPLSQSIKRLEEE
                     LGVRLFDRTRGGVVLTLAGEALLYESRKIVSQANLAVAFAHEAASKQGSRSSLRLGFI
                     SPAGYRLLPAVLRTFREVNPGVTLQLCTHPSPVLSRMVQEGELDVAFMRPLAELVRGE
                     DERLLVERTPHVWATSALEPLAARESVQLRELGEQAMIFAPAQRSPDSVSKIMAAFKV
                     AGSVPRIVQETTSTDHAMLLVRARLGSTLAPATVAHCQFADVRLIPVSDLPANTNLEL
                     VMVWHPRHLSAVGASFVAHTHELLRDKDRVNLTFATAMAPMLP"
     gene            complement(1091..1759)
                     /locus_tag="VN627_01260"
     CDS             complement(1091..1759)
                     /locus_tag="VN627_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXD01911.1"
                     /translation="MTDRAAPRPRSDRQSDTTRLAFLRAAVDLLVDRGFGALTTSRVA
                     EYAGFTRGAFQHHFASRDDLLIEVHDYVLGIDLARIPADAASLPLDQRVEVLISFLEE
                     NASSRWHAAAQALRKGIDNASPVGRAIEQRSFDMIERIHTEVIGLFPEIVMSRAEWAA
                     LIRNLAGEIFGSSFYHQWKSPELIDVEDIASRALRRRIAKLYFREISGQGSRRVVPSA
                     SGRQ"
     gene            complement(1756..3027)
                     /locus_tag="VN627_01265"
     CDS             complement(1756..3027)
                     /locus_tag="VN627_01265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD01912.1"
                     /translation="MLLNQPHHVLTPARQVAVLAVLWLLYTVHSMDRSILFLVVPAIQ
                     REFALSNQQVGFLSGLAYALPAALCAIPFGLLGDRRDRRTILALLVLAWSATTGASAF
                     AAGFAILVSLRAVVGAVESGAPPIMMSVLTDTFSAFRRPLAFSFYYTGANAGVVVAGL
                     LVHYAGKAHGWRELMMIAALPGLVLAAGLFTLVKFPAHHRDAVVQFPTTTAMTFRQVF
                     RLPGVPLLYVLCILGGMNTMAIGAWLSLFLVRGHGVDLAQAGSIVAMAQGLGGVTGGI
                     TGGLVATRFGDGGSARLCLIAGIALCISVPCGIAAGLASAITPAIVAIAGWAFFTMMF
                     VAPAHSALLQQVPPGMRATAAAVLIVGVQLVGAGIGPQVVAVLADLLGGSGEGPAIRF
                     AVAIMASVALGPAVMLIRHAVRAPTGAGEAA"
BASE COUNT          637 a         1046 c         1135 g          494 t
ORIGIN      
        1 agtttgatat atggaaccgg tagtccagcc gtggcgaggc catgctgctg ccccaagcat
       61 cgctgaccgg tctgcgcgag cgggctaaaa cgccggaatt gagttcgttc aggcttgtcg
      121 ccagttcagg gcagcatggg cgccatggct gtcgcaaaag tcaggttgac gcgatccttg
      181 tcgcgcaaca attcgtgcgt atgcgcaacg aagctggccc ccaccgcgga caggtggcgt
      241 ggatgccaga ccatcaccag ctcgaggttg gtgttggccg gcagatcact gaccgggatg
      301 aggcgaacgt ctgcaaactg gcagtgggcc acggtggccg gggccagcgt actcccgaga
      361 cgcgcgcgaa cgaggagcat cgcatgatcg gtgctggtgg tttcctggac aatgcgtgga
      421 accgatccgg cgaccttgaa ggcggccatg atcttcgaca cactgtcggg cgaacgttgc
      481 gcgggagcga agatcatcgc ctgttcgccc aactcgcgca attggactga ttccctggcg
      541 gcgagcggct cgagtgccga ggtcgcccat acatggggtg tgcgctcgac caacaggcgc
      601 tcgtcctcgc cccggaccag ttccgcaagg ggccgcatga acgcgacgtc gagttcccct
      661 tcctgcacca tccgtgacag gacgggtgaa ggatgcgtgc acagctggag ggtgacgccc
      721 ggattgacct ccctgaatgt ccgcaacacc gcgggcagca ggcgatagcc ggcagggctg
      781 atgaatccga ggcggaggct ggaccgcgaa ccctgcttgc tggcggcttc gtgggcaaag
      841 gccacggcga gattggcctg ggaaacgatc ttgcgggatt cgtagaggag cgcctctccc
      901 gcgagagtga gcaccacgcc ccctctcgtc cggtcaaaca ggcgaacgcc gagctcctct
      961 tccaggcgct tgatggactg gctcagcggc ggttgcgcca tgttcaggcg caaggcagcg
     1021 cggccgaagt gctgctcctc ggccacagcg atgaactgct tgatgcgccg gatgctgagg
     1081 tccattgccg ttattgccgc cccgatgcag aaggtaccac ccggcgacta ccctgcccgg
     1141 atatctcccg gaaatacaac ttggcgatcc tccgcctgag ggcgcgggac gcaatatcct
     1201 cgacatcgat cagctcgggc gatttccact ggtggtaaaa cgaggagcca aagatttccc
     1261 cggcgaggtt gcggatcagg gcggcccact ccgcccgcga catgacgatc tcggggaaga
     1321 gcccgataac ttcggtgtga atgcgttcga tcatgtcgaa cgagcgctgc tcgatcgcgc
     1381 ggccgactgg cgaggcgttg tcgatcccct tgcgcagggc ctgggcggcc gcatgccacc
     1441 ggctggacgc attttcttcg aggaaggaaa tcaggacctc gacccgctgg tcgagaggca
     1501 aggatgccgc atcggccgga atccgggcga ggtcgatccc gagcacataa tcgtgcactt
     1561 cgatcagcag gtcgtcccgg gaggcgaagt gatgttgaaa agcgccgcgg gtaaacccgg
     1621 catattcagc gacgcgggat gtggtgagcg cgccgaaccc gcggtcgacc agcaggtcca
     1681 cggcggcgcg caggaaggca agccgcgtgg tgtcgctctg ccggtcgctg cgggggcggg
     1741 gcgcggcgcg gtcggtcacg cggcctcccc tgccccggtc ggcgctctca cggcatgcct
     1801 gatcaacatg accgccgggc cgagggcgac gcttgccatg atggcgacag caaaacggat
     1861 ggcgggcccc tcccccgagc cgccgagcag atccgcaagg accgcgacca cctgtgggcc
     1921 gatacccgcg ccgaccaact gaactccgac gatcaggacg gcggcagcgg tggcgcgcat
     1981 gccggggggc acctgttgca ggagcgcgct gtgggccggg gccacgaaca tcatcgtgaa
     2041 aaacgcccat ccggcgatgg ccactatggc cggggtgatc gccgaggcca ggcccgccgc
     2101 gatcccgcag ggcaccgaga tgcacagcgc gatcccggcg atgagacaaa gccgggccga
     2161 gccgccatcg ccgaagcggg tggcaacaag gccgccggtg atcccgccgg ttaccccgcc
     2221 gagcccctgc gccatggcga cgatgcttcc ggcctgggcg agatcgacac cgtggccgcg
     2281 cacgaggaag agcgacagcc aggcgcctat ggccatggtg ttcatgccgc cgaggatgca
     2341 caggacatag aggagaggaa cgccgggcag gcggaacacc tggcggaagg tcattgcggt
     2401 cgtcgtgggg aactggacca ccgcgtcgcg gtggtgggcc gggaacttca ccagcgtgaa
     2461 caagcctgcc gccagcacca gcccgggcag ggctgcgatc atcatgagct cccgccagcc
     2521 atgggctttt ccggcgtaat ggaccagcaa gcccgcgacg acgaccccgg cattggcgcc
     2581 ggtgtagtag aagctgaacg cgaggggccg gcggaatgcg gagaaggtat cggtgagaac
     2641 cgacatcatg atcgggggcg cgccagattc gaccgctccc accacggcgc gcaagctgac
     2701 gaggatggca aagccagcgg caaaggcact agcccccgtg gttgcgctcc acgccagtac
     2761 gaggagggcc aggatcgtcc ggcggtcacg ccgatcgccg agcaggccga acggaatggc
     2821 gcagagcgca gccggcaggg cataggccag tcccgaaagg aatccgacct gctggttgga
     2881 cagggcgaat tcgcgctgga tcgccgggac caccaggaac aggatgctgc gatccatgct
     2941 atggaccgtg tacaggagcc acaacacggc cagcacggcg acctggcgcg ccggcgtcag
     3001 cacgtgatgc ggctggttca ggagcattgg gcgcggtcag ccggggcaac cgccgacggg
     3061 atcggccggg gcggggtgcg cacgtccgag tagttcatgc ctgactggcg attacccggc
     3121 atcctccacc gcccctcgtg ccctgattgc ccgcgaacct gcgggcgacg cagacccatg
     3181 cataattgat ttttccccgt gcgcaacgtg caggtcacgc cggcggagag cggaaatgct
     3241 tggccggcaa gccatcaata cgccccccgc atatcaggga aatccgctag gttgactcat
     3301 acattcaaaa at
//
LOCUS       DATNBV010000053         2535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_352189,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000053 DATNBV010000000
VERSION     DATNBV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352189"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VN627_01270"
     CDS             complement(<1..554)
                     /locus_tag="VN627_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01913.1"
                     /translation="MIRKTTLIARTALAAALAFGTVGTAFVAAPAMAKEAAPKLSPGF
                     VKLAGPFQTELGALQKAPDAAKANALVPKWEALAAAASTPDDKFFAGQFGIAVSQMSK
                     NTDLQTRALQLTVDSGKVSGPELGKYLFYLGAAKINAKDFAGAQAALTKAQAAGATDP
                     DLPALIAETYFGQNQNQAGLDALWA"
     gene            885..>2535
                     /locus_tag="VN627_01275"
     CDS             885..>2535
                     /locus_tag="VN627_01275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HXD01914.1"
                     /translation="MSDTTDIIDPAAQPGGQPGEFTRVDIVDEMKTSYLDYAMSVIVS
                     RALPDVRDGLKPVHRRILFASNEGGFVAGRPYRKSAKIVGDTMGNYHPHGDSSIYDAL
                     ARMTQDWSMRVPLIDGQGNFGSMDPDPPASMRYTEARLTRVANFLLDDLDKDTVDFTD
                     NYDGSRQEPTVLPARFPNLLVNGAGGIAVGMATNIPPHNLGEVIDGCLAMIENPAITT
                     DQLIEIIPGPDFPTSPMILGTAGARSAYHTGRGSIIMRANHKIEEGRGDRRSIVLTSI
                     PYQVGKNGLVEKIAEAAKDKRIDGVADIRDESNREGVRVVIDLKRDATPEVVLNQLWR
                     NTPAQSNFPANMLAIRGGRPEILSLRDIIQSFIAFREEVITRRTKFELNKARDRAHIL
                     LGLVVAVTNMDEVVRIIRGAPNPGAARTALLAREWPIGEIAPYIRLVEAIEPDAEQEG
                     GTYRLSEVQVKAILDLRLHRLTALGRDEIGDELKELAAAIEEYLSILADRKKLYAVMT
                     EELNQVRQLYATPRMSQIAPAWDGIDDEDLIERDEMVVTVTL"
BASE COUNT          465 a          847 c          808 g          415 t
ORIGIN      
        1 gcccacaggg catcgagacc ggcctggttc tggttctggc cgaaataagt ctcggcgatc
       61 agggcgggga gatcgggatc ggtcgcaccc gcggcctggg ccttggtgag cgccgcctgg
      121 gctcccgcga aatccttggc gttgatcttg gcagcgccga gatagaacag gtacttgccg
      181 agttccggac cgcttacctt gccgctatcc acggtgagct gaagcgcgcg ggtctggaga
      241 tcagtgttct tgctcatctg gctgacggcg atgccgaact ggccggcaaa gaacttgtcg
      301 tccggcgtgg aagcagcggc ggcgagggct tcccacttgg gcacaagcgc gttggccttg
      361 gccgcatccg gcgccttctg cagggcgccc agttcggtct ggaacggacc agccagtttg
      421 acgaacccgg gcgaaagctt gggcgccgct tccttggcca tggccggagc cgccacgaaa
      481 gcggtgccga cagtgccgaa tgcgagggca gccgccagtg cggtgcgggc aataagcgtg
      541 gtcttgcgga tcatggtgcc ttttcgtctc ctcttcgccg ccgccccgaa gggaaggtcg
      601 ggcgcaatgt agccccaaaa cattctgcct gcgcaaatgg cccgatatcg ggcccttggc
      661 aaccacatcg gcgatatttg cttttgcttg aacgcggatt gaatggcgcg gctcccgccc
      721 gttcatgatc ggttggcgca tgccgcccgg ctgcccggca agaggtgtgg aaaaatggct
      781 ctgccactcc cttcgacaag gggttccgct ggccttgccg ggcgccatcg cctagggcgg
      841 tgggacgccg tttcccacga acaacataaa gatcctgcgc agacgtgagc gacaccaccg
      901 acatcatcga tcctgccgcg cagccgggcg gccagccggg cgaattcacc cgcgtcgaca
      961 tcgtcgacga gatgaagacc agctacctcg actacgcgat gagcgtgatc gtcagccgcg
     1021 ccctgccgga cgtgcgcgat ggcctcaagc cggtgcaccg ccgcatcctg ttcgcctcga
     1081 acgagggcgg gttcgtcgcc ggacgaccct atcgcaagag cgccaagatc gtcggcgata
     1141 ccatgggtaa ctaccacccc catggtgaca gctcgatcta cgatgccctc gcccgcatga
     1201 cccaggactg gtcgatgcgc gtgccgctga tcgacggcca gggcaacttc ggctcgatgg
     1261 accccgatcc cccggcctcg atgcgctaca ccgaggcgcg cctgacccgt gtcgccaatt
     1321 tcctgctcga cgatctcgac aaggacacgg ttgacttcac cgacaactac gacggttccc
     1381 gccaggagcc gacggtgctg ccggcccgct tccccaacct gctggtcaac ggcgcgggcg
     1441 gcatcgcggt gggcatggcg accaacatcc caccgcataa cctgggcgaa gtgatcgacg
     1501 gctgcctcgc catgatcgag aacccggcga tcaccaccga ccagctgatc gagatcatcc
     1561 cgggtccgga cttcccgaca agcccgatga tcctcggcac ggccggcgcg cgcagcgcct
     1621 atcacaccgg ccgtggctcg atcatcatgc gggccaacca caagatcgag gaaggccggg
     1681 gcgaccgccg ctcgatcgtg ctgacctcga tcccttacca ggtcggcaag aacggcctgg
     1741 tcgagaagat cgccgaggcc gccaaggaca agcggatcga cggcgtcgcc gatatccgcg
     1801 acgagtccaa ccgcgaaggc gtgcgcgtgg tcatcgacct caagcgcgat gcgacccccg
     1861 aagtcgtact caaccaactc tggcggaaca cgccggctca gtcgaacttc ccggccaaca
     1921 tgctggcgat ccgcggcggg cggccggaaa tcctctcgct gcgcgatatc atccagtcgt
     1981 tcatcgcctt ccgcgaggaa gtgatcaccc ggcgcaccaa gttcgagctg aacaaggcgc
     2041 gtgaccgggc gcacatcttg ctcggcctgg ttgtcgccgt gaccaacatg gacgaggtgg
     2101 tgcgaatcat ccgtggcgcg cccaatcccg gagccgcccg taccgccctg ctcgcgcgcg
     2161 aatggccgat cggagagatc gcgccctaca tccgcctggt tgaggcgatc gagcctgatg
     2221 ccgaacagga aggcggcacc taccgcctgt ccgaggttca ggtgaaggcg attctcgact
     2281 tgcgcctgca ccgcctgacg gcgctcggcc gcgacgagat cggcgacgag ctcaaggaac
     2341 tggccgccgc gatcgaggaa tacctgtcga tcctggctga ccggaagaag ctctatgcgg
     2401 tgatgaccga ggagctgaac caggtccgcc agctctatgc cacgccgcgc atgtcgcaga
     2461 ttgccccggc ctgggacggg atcgacgacg aggacctgat cgagcgcgac gagatggtcg
     2521 tgacggttac gctgg
//
LOCUS       DATNBV010000054         3029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_352537,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000054 DATNBV010000000
VERSION     DATNBV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352537"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VN627_01280"
     CDS             complement(<1..568)
                     /locus_tag="VN627_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HXD01915.1"
                     /translation="MHSYPDASAVVRALAPDEPVIINRPHAAARAARFFVEKFPGKSL
                     YAVKANPSPDLLRVLWDAGVSHYDVASIGEVRMVRATLPEATLCFMHPVKTASAIAEA
                     YKVHGVRTFSLDCQEELDKIIAATASEDGTPAPDLRLCVRLRVSSEYSELSLASKFGI
                     DLADAAPLLQATRQVSDWLGICFHVGSQA"
     gene            complement(803..1846)
                     /locus_tag="VN627_01285"
     CDS             complement(803..1846)
                     /locus_tag="VN627_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXD01916.1"
                     /translation="MKPRLAFLGTGWIGRSRMEAILADGAGEAVALCDPSDECAELAL
                     AFAPEAVRTETLEGLLELAPDGLVIATPSAQHAAEAITALNRGVPVFCQKPLGVSAGE
                     VRRVVGAARRADCLLGVDFSYRLAAAFAEAAALVRAGSLGKVHTVDLTFHNAYGPDKP
                     WFYDRALSGGGCLMDLGVHLADLALWLFGYPDVEVAAAALTAGGRPATAEDVEDFALA
                     TLKLAGGTTVRLACSWRAHAGCDAVIAAEFHGTSGGALVRNVGGSFHDFEAFRFTGTA
                     REQIAAPPDAWGGRAAVAWARRLAGSHAYDPAAEGFITTAEAIEAIYAAGLAGIGSAE
                     SPRQARAPSRSHG"
     gene            complement(1843..2820)
                     /locus_tag="VN627_01290"
     CDS             complement(1843..2820)
                     /locus_tag="VN627_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXD01917.1"
                     /translation="MTARTMHAAVLTGPGTIELARVPVPEPGPGQVRVALEGCGVCAS
                     NVEPWLGQPWSTWPGEPGGLGHEGWGTIDAVGDGVDLVPGTRVATLSGRSFAEYDLAA
                     PEQLIALPQPLDGQPFPAEPFGCAVNVAARADFQPRQWVAVIGAGFIGAVVTRLAAQA
                     GARVIAISRRASSRALARTMGAEVTVAMDDHWRIIEAVRTITGDELCPRVVECVGAQW
                     PLDLATELVGFGGRLVVAGYHQDGPRQVNMQAWNWKGIDVINAHERDPAVQRRGAERA
                     AEAVASGEIAAEPLLTHRFGLDRLGEALAATRDKPEGFVKAIVDLGVML"
BASE COUNT          492 a         1123 c          999 g          415 t
ORIGIN      
        1 tcgcctgcga gccaacgtgg aagcagatgc cgagccagtc ggaaacctgg cgcgtcgcct
       61 gcagcagcgg ggccgcgtcg gccagatcga tcccgaactt cgaggccagg ctcagctccg
      121 aatattcgga ggagacgcgc agccgcacgc acaggcgcag gtcaggcgca ggcgtcccgt
      181 cctcgctcgc tgtggccgcg atgatcttgt ccagctcttc ctggcaatcg aggctgaagg
      241 tgcgcacgcc gtggaccttg taggcctcgg caatcgcgct ggcggtcttc accgggtgca
      301 tgaagcacaa ggtggcctct ggcagggtgg cgcgaaccat ccgcacttcg ccgatcgagg
      361 cgacatcgta gtggctcacc ccggcgtccc acagcacgcg cagcaggtcg ggcgagggat
      421 tggccttgac cgcatagagc gacttgcccg gaaacttctc gacaaagaag cgggcagccc
      481 gcgcggcggc gtggggacga ttgatgatga ccggttcgtc gggcgcgagc gcgcgaacta
      541 cagccgatgc gtcaggatag ctgtgcaact caagggaccc ccaataggcc tttcggccat
      601 gcaacaacac aaagctgcct tgcggtttgg aagtccccat ggggcagcga gagggggcga
      661 tatagggtgc ctgcgcttaa tcgcaaatga aaaatggcca cgcctttttc gggggcgcgc
      721 ggggggagga aaacggctca aatccagcag tttggcgcac cttctggcgg ggtggcccga
      781 agtgccccca aagcccgccg cctcagccgt gcgaacgact cggcgcccgg gcctgacggg
      841 gggattcggc agagccgatt cccgcaaggc ccgcagcgta aatcgcctcg attgcctcgg
      901 ccgtggtgat gaacccctcc gccgccgggt cataggcgtg cgagccggcc agccgccgcg
      961 cccatgccac agccgcgcgg ccgccccagg catcgggcgg ggcggcgatc tgctcccgcg
     1021 ccgtgccggt aaagcggaag gcctcgaagt catggaagct gccgcccacg ttgcgcacca
     1081 gcgccccgcc ggaggtgcca tggaactcgg cggcaatcac cgcatcgcag ccggcatggg
     1141 cccgccagga gcaggccagc cgcaccgtcg tgccgccggc cagcttcagc gtggccaagg
     1201 caaagtcctc aacatcctcg gccgtcgccg ggcgcccgcc cgctgtcagc gccgccgcgg
     1261 ccacttccac gtccggatag ccgaacagcc acagcgccag gtccgccaga tgcaccccca
     1321 ggtccatcag gcacccgccg cccgagagcg cgcggtcgta gaaccacggt ttgtccggcc
     1381 cgtaggcgtt gtgaaacgtg agatcgaccg tgtggacctt gcccagcgag cccgcgcgaa
     1441 ccagcgccgc cgcctcggca aaggctgccg caagccggta ggagaaatcc acgccgagca
     1501 ggcagtccgc ccgccgcgct gcgccgacca cccggcgcac ttcgccagcg ctcaccccca
     1561 gcggcttctg gcagaacacc ggcacgcccc ggttgagagc ggtgatggct tcggccgcat
     1621 gctgggcgct gggcgtggcg atcaccagcc cgtccggcgc cagctcgagc aagccctcaa
     1681 gcgtttccgt tcgcaccgct tcgggcgcaa aggcgagagc gagctctgca cactcgtccg
     1741 aagggtcgca cagcgccacg gcctcgccgg cgccatcagc caggatcgcc tccattcgcg
     1801 aacgcccgat ccagccggtg ccgagaaagg cgaggcgcgg cttcacagca tcaccccaag
     1861 gtccactatc gctttgacga agccttccgg cttgtcccgc gtcgccgcca gcgcctcgcc
     1921 cagccggtcc agcccgaagc ggtgagtcag cagcggctcg gccgcgatct cgccgctggc
     1981 gactgcctcc gctgcccgct ccgccccgcg ccgctgcacg gccggatcgc gctcgtgggc
     2041 attgatcacg tcgatcccct tccagttcca cgcctgcatg ttgacctgcc gcggcccatc
     2101 ctggtgatag ccggccacga ccagccgtcc gccgaagccg accagctcgg tggcaaggtc
     2161 cagcggccac tgcgcgccaa cgcattcgac cacccggggg cacagctcgt cccccgtaat
     2221 ggtgcgcacg gcctcgatga tccgccagtg atcgtccatc gccacggtca cttccgcgcc
     2281 catggtccgc gccagcgccc gcgagctcgc ccggcgggag atggcgatca ccctggcccc
     2341 ggcctgcgcg gcgagccgag tcaccaccgc gccgatgaac cccgcgccga tcaccgccac
     2401 ccactggcgc ggctggaaat ccgcccgcgc cgccacgttc accgcgcagc cgaatggctc
     2461 ggccgggaag ggctgtccgt ccagcggttg aggcaaggca atcagctgct ccggcgcggc
     2521 aaggtcgtat tcggcgaagg atcgccccga cagcgtggcc acccgcgtgc ccggcaccaa
     2581 gtccacgccg tcgcccaccg cgtcgatcgt accccagcct tcgtgaccaa gcccgccggg
     2641 ctcccctggc catgtcgacc agggctggcc gagccacggc tccacgttcg aggcgcagac
     2701 cccgcaccct tccagggcca cgcgcacctg tcccgggccc ggctcgggca ccggcacccg
     2761 ggcaagttcg atcgtgccgg gcccggtgag cacggcggca tgcatggttc gtgcggtcat
     2821 ggggtgcaac ctgaggcgcc cgcagggcgt tggaggggga tggacggcat gaaccgcact
     2881 ctgcgcgaca acatcgaggc cttcgagcag cgccggcgcg acagtgatgc gcgggccggg
     2941 ccgggcgaac ggttctcgcg cctcgtcacc cggttcgccg gctcgatcac cttcgtcgcg
     3001 gtccacgcgg tggccttcgg cctgtgggt
//
LOCUS       DATNBV010000055         5579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_49968,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000055 DATNBV010000000
VERSION     DATNBV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49968"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VN627_01295"
     CDS             <1..416
                     /locus_tag="VN627_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HXD01918.1"
                     /translation="KVNPTQCEMLTMVAGQVIGNHMAVTVGGMQGHLELNVFKPLIGA
                     NILRSVDLLATGMDSFAARCVEGLQPNGARIADLVSRSLMLVTALAPEIGYDNAARIA
                     KHAHAKGQTLREAALELGLVDGPTFDRLVRPENMV"
     gene            497..1102
                     /locus_tag="VN627_01300"
     CDS             497..1102
                     /locus_tag="VN627_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXD01919.1"
                     /translation="MSIFDKLAARIMPPESAQDRANARKVAESLAGDGDWLAIVLEHH
                     RQIEAFFTQAMVAPDGPSRLNALKRLAVLLTGHANAEETVLYPALAQGGEKGHAAMAY
                     QEQAMAKVEMALLEKLDPMGRDWREKLEHIQGAVLHHVYEEEGTWFAELQQQLPTAER
                     PRLTRRFVEEFERYTRGPSQHETPMQMAAQQKGTPSAPMGK"
     gene            complement(1258..1818)
                     /locus_tag="VN627_01305"
     CDS             complement(1258..1818)
                     /locus_tag="VN627_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HXD01920.1"
                     /translation="MKPKDLPEESRELADFAVRVMNKVGEARLSVVTAESCTGGLLAS
                     LLTDLPGLSNGFERGFVTYTEEAKCELLGIDPVLIARHGVVSREIAAAMADGALAHSR
                     GKVGVGITGYAGPGPTNQHQGEAGLVHVAARRKGGRLYSRECHYGDIGRDRVRMLAAH
                     AALELIEEAVGELVASKPRPGEQTEP"
     gene            complement(1915..4440)
                     /gene="ligD"
                     /locus_tag="VN627_01310"
     CDS             complement(1915..4440)
                     /gene="ligD"
                     /locus_tag="VN627_01310"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HXD01921.1"
                     /translation="MATKDKLAEYNRKRDFTRTAEPSGKVAQTGGNGFIVQKHDATRL
                     HWDFRLEVDGVLKSWAVTKGPSADPEEKRLAVRTEDHPLAYAEFEGTIPKGEYGGGTV
                     MLWDKGTWEPIAGKSAKDLEKGHLHFVLHGERMKGEWIIFRLKPRPGEKRENWLLRKV
                     TDAYAASGDTLVERGLTSVLTGRTMAEIAADAGGAHSLKGKSGKAFAAEMDKAAQRNA
                     ALKPKPAKRAKGLPEFRPVQLATLVDAVPAGNGWMHEVKFDGYRALVAVNGGEVKVFT
                     RNGLDWTDKFQPLARALAAMDLPRALIDGEIVAPGKDGNPSFSALQAILKRGHSSQGE
                     DNPLQFHAFDLLELDGEDLSGRPNLERKDRLGALLATAQPPVFFANHVIGAGEKLFEA
                     MCGAGGEGIVSKRIDGSYLAKRGRNWVKVKCIRRQEFVVIGWKKSSARARPFASLLLG
                     QYDEGTLTYTGNVGTGFDEKTLREVAEAMAPLVSDTAPAEVPKADRRGVTWLKPQLVA
                     EVAFAEFTADGSVRHASFLGLRADKQAKDVVPERAEHLAEGGDTLPESPVKISNRGRV
                     IYPESGLTKGELADYYLQIAPLMLPTMGGRPISLIRCPQGRAKQCFFQKHDAGTFGPH
                     VHHVPITEKDGHLEEYIFVGDAAGILSCVQMGAIEFHGWCSLARDVEAPERLVIDLDP
                     DEGLGFDAVNAAARDIRRKLSDLGLVSFAMLSGGKGVHVVVPLTPGHTWAQHSDFAHR
                     FAGALALGDPDNYVATMSKAKRTGKIFVDWLRNQRGSTAVMPYSARARENAPVAVPVS
                     WEELDGLASAHEFTIRDAGKLLERAAGKALQGWGLAAQTLPQV"
     gene            complement(4440..5330)
                     /locus_tag="VN627_01315"
     CDS             complement(4440..5330)
                     /locus_tag="VN627_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXD01922.1"
                     /translation="MAARAYWSGQIRLALVSIPVEIYPATRSAGRIGFNLIHEPTGQR
                     VRYQKVVPGVGEVDPDEIVKGFEVSKGNYVLLEEKEIEAAKVESRRTLDLVQFVDAGE
                     IDVLYFEKPYFVVPADDLAEEAYVVLRDALKAARKVGVGQISVRGREHLVALKPCGRG
                     MVLEILRYADEVQKAHGFFAEIGEDKPQAELLQLATSLIEQKSGSFEPGKFHDRYEDA
                     LKRLIEKKTKAKGKLVLEDVQAPAPRGGNVIDLMAALKNSLGGGGAPPEPAEAKPARK
                     RPAAPAKKPAAETPAKRKRA"
     gene            complement(5365..>5579)
                     /locus_tag="VN627_01320"
     CDS             complement(5365..>5579)
                     /locus_tag="VN627_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01923.1"
                     /translation="GDRLHWRAYGQFMAGGALGTPLSWLLVMTMRKGLGWPMEWTAPA
                     MTVLMLIYNYLNARAAIHWRRRKAAG"
BASE COUNT          894 a         2129 c         1613 g          943 t
ORIGIN      
        1 gcaaggtcaa cccgacgcag tgcgagatgc tgacgatggt cgccgggcag gtgatcggca
       61 accacatggc ggtgacggtc ggcgggatgc aggggcatct cgaactcaac gtgttcaagc
      121 cgctgatcgg cgccaacatc ctgcgctcgg tcgacctcct cgccaccggc atggactcct
      181 ttgccgcccg ctgcgtcgaa ggcctccagc ccaacggcgc ccgcatcgcc gacctcgttt
      241 cccgctcgct gatgctggtc accgcgctcg cccccgagat cggctacgac aacgccgccc
      301 ggatcgccaa gcacgcccac gccaaggggc agaccctgcg cgaagccgcg ctcgaactcg
      361 gcctcgtcga cggccccacc ttcgaccggc tggtccgccc cgaaaacatg gtctgacgtt
      421 cgccagcagc gctcacgcgc ggaacaggcg gcgcccccgt cctttggttg gtcatccact
      481 ccattggaga ccattcatgt ccatcttcga caagctcgcc gccaggatca tgccgcccga
      541 gtccgcgcag gaccgcgcca atgcccgcaa ggtcgccgaa agcctcgccg gcgatggcga
      601 ttggctggcc atcgtgctcg aacatcaccg ccagatcgag gccttcttca cccaggccat
      661 ggtcgccccc gacggcccga gccgcctcaa tgccctcaag cgtctcgccg tgctcctcac
      721 cggccatgcc aatgccgagg aaaccgtgct ctatcccgcc ctcgcccagg gcggcgaaaa
      781 gggccacgcc gccatggcct accaggaaca ggcgatggcc aaggtcgaaa tggccttgct
      841 cgagaagctc gaccccatgg gccgggactg gcgcgaaaag ctcgaacaca tccagggcgc
      901 cgtgctgcac cacgtctacg aggaggaagg cacctggttc gccgaactcc agcagcaact
      961 ccccaccgcc gaacgcccgc gcctgacccg ccgcttcgtg gaagaattcg aacgctacac
     1021 ccgcggcccc agccagcacg aaaccccgat gcaaatggcc gcccagcaaa aggggacgcc
     1081 atccgccccg atgggcaagt aagccaatag gttgctccct ccccagcggg aggggtttcc
     1141 ttctcttctc cctccccggc gggaggggtt tccttctctt ctcccctccc cggcggggag
     1201 gggttggggg agggggtttt gctccagcaa tgccgaacac ccccacccaa ccctccctca
     1261 cggttccgtt tgttcgccgg gccttggttt ggaggcgaca agctcgccca ctgcctcctc
     1321 gatcagctcc agcgcggcat gggccgccag catgcgcacc cggtcgcggc caatatcgcc
     1381 gtagtggcat tcgcgcgaat agagccgccc acccttgcgc cgcgcggcca cgtggaccag
     1441 cccggcctcg ccctggtgct ggttcgtggg gccaggcccg gcatagccgg tgatccccac
     1501 gcccaccttg ccccggctgt gcgccagggc tccatcggcc atggccgcgg caatctcccg
     1561 gctcaccacg ccatgccgcg cgatcagcac cggatcgatc cccagcagct cgcacttggc
     1621 ctcttcggta taggtgacga acccccgctc gaacccgttg ctgagtcccg gcaggtcggt
     1681 cagcagcgag gccagcagcc ccccggtgca ggactccgcg gtgacgaccg acaggcgggc
     1741 ctcccccacc ttgttcatca cccgcacggc aaaatcggca agctcacggc tctcttcagg
     1801 caagtccttg ggtttcatgg gaaatttgac gcgtgaaggc gggggcggtt cctgccgtga
     1861 cttacatcga acccgttcgt gtcgagcgaa gtcgggacac tgctggcaag tccctcaaac
     1921 ctgcggcagc gtctgcgccg ccagccccca gccctgcaac gccttccccg ccgcccgctc
     1981 cagcaatttc cccgcatccc ggatcgtgaa ctcatgcgcg ctggcgagcc cgtcgagctc
     2041 ctcccagctc actggcaccg ccaccggcgc attctcccgc gcccgtgccg aatagggcat
     2101 gaccgcggtc gacccgcgct ggttcctcag ccagtcgacg aagatcttcc ccgtccgctt
     2161 ggccttgctc atcgtcgcca cgtagttgtc cgggtcgccg agggcgagcg cgccggcaaa
     2221 gcggtgcgca aaatcggagt gctgcgccca ggtatgcccc ggggtcagcg gcaccaccac
     2281 gtgcaccccc ttgccgcccg aaagcatggc gaacgacacc agccccaggt cggaaagctt
     2341 gcgccggatg tcccgcgccg ccgcgttgac cgcgtcgaac cccagcccct cgtcgggatc
     2401 gaggtcgatc accagccgct ccggtgcctc gacatccctc gccagcgaac accacccgtg
     2461 aaactcgatc gcccccatct gcacgcatga caggatcccc gcagcatccc cgacgaagat
     2521 gtattcctcg agatgtccat ccttctcggt gatcggcacg tgatgaacat gcggcccgaa
     2581 ggtcccggca tcgtgcttct ggaaaaagca ctgcttggcc cgcccttgcg gacagcggat
     2641 caggctgatc ggccgcccgc ccatggtcgg cagcatcagc ggcgcgatct gcaaatagta
     2701 gtcggcgagc tcgcccttgg tcagtccgct ctcgggatag atcacccggc cccggttgct
     2761 gatcttcacc gggctctcgg gcagcgtgtc gccaccctcg gcaagatgtt ccgcgcgctc
     2821 cggcaccacg tccttcgcct gcttgtcggc gcgcaggccc aggaagctcg cgtgccggac
     2881 cgatccatca gccgtaaact cggcaaaggc aacctcggcc accagctgcg gcttcagcca
     2941 ggtcaccccc cgccggtccg ccttgggcac ttcggccgga gccgtgtcgc tcaccagcgg
     3001 cgccatggcc tcggccacct cgcgcaaggt cttctcgtcg aacccggtgc cgacattgcc
     3061 ggtgtaggtc agcgtaccct cgtcgtactg cccgagcagc agcgatgcaa agggccgcgc
     3121 ccgggcgctc gatttcttcc acccgatcac cacgaattcc tgccgccgga tgcacttgac
     3181 cttcacccag ttgcgcccgc gcttcgccag gtacgagcca tcgatccgtt tcgaaacaat
     3241 gccttccccg cccgccccgc acatcgcctc gaacagcttc tcccccgccc cgatgacgtg
     3301 attggcgaag aacaccggcg gctgggcggt ggccagcaag gcccccagcc gatccttgcg
     3361 ctcgaggttc ggccggccgg acaggtcctc accgtccagc tccagcagat caaacgcatg
     3421 gaattgcagc ggattgtcct caccctgcga actatgcccg cgcttcagga tcgcttgaag
     3481 tgccgaaaaa ctggggttcc catccttccc cggcgccacg atctcgccat cgatcagcgc
     3541 ccgcggcagg tccatcgccg ccagggcgcg ggccagcggc tggaacttat ccgtccagtc
     3601 caggccattg cgggtaaaca ctttcacctc accgccattg accgccacca gtgcccggta
     3661 accgtcgaac ttcacctcgt gcatccagcc attgcccgcc ggcaccgcat cgaccagcgt
     3721 cgccagctgc accgggcgaa actccggcag ccccttggcc cgcttcgccg gcttcggctt
     3781 cagcgcggca ttcctctgcg ccgccttgtc catttcggcc gcgaaggcct tgccgctctt
     3841 gcccttaagg ctgtgcgcgc cccccgcatc ggcggcgatc tcggccatgg tccgccccgt
     3901 caggacggag gtcagccccc gctcgaccag cgtgtcgccg ctcgcggcat aggcatcggt
     3961 caccttgcgc agcagccagt tctcgcgctt ctcgccgggg cggggcttga ggcggaagat
     4021 gatccactcc cccttcatcc gctcgccatg gagcacgaag tggaggtggc ccttctccag
     4081 gtccttggct gacttgccgg caatcggctc ccacgtcccc ttgtcccaca gcatcaccgt
     4141 gccgccgcca tattcgccct tggggatcgt cccctcgaac tcggcatagg ccagcgggtg
     4201 atcctcggtc cgcacggcca gccgcttctc ctcgggatcg gcgctcggcc ccttggtcac
     4261 ggcccaggat ttgagcacgc cgtccacttc cagccggaag tcccagtgca gccgggtcgc
     4321 atcgtgcttc tggacgatga agccattgcc gccggtctgc gccactttcc ccgaaggctc
     4381 ggcggtgcgc gtgaaatcgc gcttgcggtt gtattcggca agcttgtctt ttgtcgccat
     4441 cacgcccgct tccgcttggc cggggtctcc gccgccggct tcttggcggg agccgcgggc
     4501 cgcttgcgcg ccggcttcgc ctcagccggc tcgggcggag caccaccacc gcccagcgaa
     4561 ttcttcagcg ccgccatcag gtcgatcaca ttgccgccgc gcggcgccgg tgcctgcaca
     4621 tcctccagca ccagcttgcc cttggccttg gtcttcttct cgatcagccg cttcagcgca
     4681 tcttcatatc ggtcgtggaa cttgcccggc tcgaagctgc cgctcttctg ctcgatcagg
     4741 ctcgtcgcca gctgcagcag ctcggcctgc ggcttgtcct cgccgatctc ggcaaagaac
     4801 ccatgggcct tctgcacctc gtcggcatag cgcaggatct ccagcaccat gccgcgcccg
     4861 caaggcttca gcgccaccag gtgttcacgc ccgcgcaccg agatctgccc cacgcccacc
     4921 ttgcgcgcgg ccttgagcgc atcgcgcagc accacatagg cctcctcggc caggtcatcc
     4981 gccgggacca cgaaataggg cttctcgaaa tagagcacgt cgatctcgcc cgcgtcgacg
     5041 aactgcacga gatcgagcgt ccgccggctc tccactttcg ccgcctcgat ctccttctcc
     5101 tcgagcaata cgtagttgcc cttggaaacc tcgaagccct tgacgatctc gtcgggatca
     5161 acctcgccca cgcccgggac caccttctgg tagcgcaccc gctgccccgt gggctcatgg
     5221 atcaggttga acccgatccg cccggccgaa cgggttgccg ggtagatttc gaccgggatc
     5281 gagacgaggg ccagcctgat ctggcccgac cagtatgcgc gtgctgccat gcagggggag
     5341 cgcgcgagac cggcgaatgt tccgtcagcc cgccgccttg cgccgtcgcc agtggatcgc
     5401 cgcccgagcg ttgagatagt tgtagatcag catcagcacg gtcatcgccg gcgcggtcca
     5461 ttccatgggc cagccaaggc ccttgcgcat ggtcatcacc agcagccacg acagcggcgt
     5521 gcccagcgcc ccgccggcca tgaactggcc gtaggcccgc cagtgcagcc ggtccccat
//
LOCUS       DATNBV010000056         2941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4521055,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000056 DATNBV010000000
VERSION     DATNBV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4521055"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VN627_01325"
     CDS             <1..961
                     /locus_tag="VN627_01325"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXD01924.1"
                     /translation="EGVTHVAYEASSHGLSQYRSEGLRVAAGAFTNLSRDHLDYHRDM
                     ADYFDAKMRLFDEVVDGDGAAVLWADDAWSAAASERARTRGLRLLTVGEKGDTLRLLA
                     RSPSQLGQVLKIMHEGAEHTVNLPLIGAYQAANAMVSAGLVLATGGEAAQTLAALERL
                     QPVRGRLERAAIARSGAPVYVDYAHTPDALEAAIAALRPHVKGRLIVVFGAGGDRDSG
                     KRPEMGRVAAARADLAIVTDDNPRGEDPAEIRKSVLAGCPGAREIGERREAIATAIGE
                     ATGDDIVLVAGKGHEQGQIVGRGADTRVLPFDDVAVAREYALS"
     gene            958..2424
                     /gene="murF"
                     /locus_tag="VN627_01330"
     CDS             958..2424
                     /gene="murF"
                     /locus_tag="VN627_01330"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HXD01925.1"
                     /translation="MNAAVALIQWPEDSFDDWPLALWTSDELAEACGGVASGPFQVSG
                     VEIDSRDVVEGDLFFALKGETMDGHRFLEGAFAKGAAAAVVDRKIPGNHILVADTSVA
                     LERLAQAARARIASTSVVLGVTGSVGKTSVKEALFAALDRASHGRAHRSVKSYNNHVG
                     VPLSLSRMPAGTQFGVFEMGMNHAGEIDGLTRQVRPHIALITTIAPAHIENLGSEEAI
                     ADAKAEIFRGLEPGGTAIIPADSPHFARLRAAALLLQAEVVSFGRADHADVRLLDAVP
                     APGGGSLVTIDMGDRRLCYTVAAPGEHWVTNSLAVMAAVRAAGGDLGAAGLALGELAG
                     MKGRGARHELPTPDAGTALLIDESYNANPASMTATLAQLGATPAIRRIAVLGAMKELG
                     EHGPAMHADLAGPLAAAQVDHAILVGDEMGALADELGKSAGPALGKTVQFSHCPDTAA
                     AIAALADLGLASGDAILVKGSNSVGLGKLVAALLDDKG"
     gene            2437..>2941
                     /gene="mraY"
                     /locus_tag="VN627_01335"
     CDS             2437..>2941
                     /gene="mraY"
                     /locus_tag="VN627_01335"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413324.1"
                     /note="First step of the lipid cycle reactions in the
                     biosynthesis of the cell wall peptidoglycan; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXD01926.1"
                     /translation="MLYLLAEWMHFPGLANLIRYQSFRAGAALITAMLIGLVIGPRFI
                     NMLRVRQGKGQPIREDGPATHLAKRGTPTMGGLMILISLVISMLLWMDLTNPFVWACL
                     AVTAGFGVIGFMDDFDKVTKYSHKGVPGRVRLAMEFVVAGIAAWIIVSQISTNLYVPF
                     LSGRYIPL"
BASE COUNT          451 a         1075 c          987 g          428 t
ORIGIN      
        1 cgagggggtg acccatgtgg cctatgaggc ttcgagccac ggcctgtcgc aataccgcag
       61 cgaaggactg cgggtggcgg caggagcctt caccaatctc agccgcgacc atctcgacta
      121 ccacagggac atggcggact acttcgacgc caagatgcgc ctgttcgacg aggtggtgga
      181 cggggacggc gcggccgtcc tctgggccga cgacgcatgg tcggcggcgg cgagcgagcg
      241 ggcgcggacg cgggggttac gcctgctcac ggtgggcgag aagggggaca cgctccgcct
      301 tctcgcccgc tcccccagcc agttggggca ggttctgaag attatgcatg aaggggcgga
      361 gcacacggtc aacctgccgc tgatcggggc ctaccaggcc gcgaacgcga tggtttcggc
      421 cggcctggtg ctcgccacgg gcggcgaggc ggctcagacc ctcgccgccc tggagcggct
      481 gcagccggtg cgcggccggc tggagcgcgc cgccatcgcc cgctcgggcg cgccggtcta
      541 tgtcgactat gcccacaccc ccgatgcgct cgaagcagcc atcgccgcgc tgcggcccca
      601 tgtgaaaggg cggctgatcg tggtattcgg tgcgggcggg gatcgcgatt ccggcaagcg
      661 gcccgagatg gggcgcgtcg ccgcggcccg ggccgatctc gcgatcgtca ccgacgataa
      721 tccgcgcggc gaagatccgg cggagatccg caagtcagtg ctcgccggat gtccgggagc
      781 gcgtgagatc ggcgagcggc gggaagccat cgccacggcc atcggggaag cgacggggga
      841 cgatatcgtc ctcgtcgccg gcaagggaca cgaacagggg cagatcgtcg ggcgaggcgc
      901 ggacacgcgc gtcctgccgt tcgacgatgt tgccgtagcc agggaatacg cgctgtcatg
      961 aacgctgccg ttgccttgat ccagtggccc gaggactcct tcgacgactg gccgctcgcc
     1021 ctgtggacct ccgacgaact ggccgaagct tgtggcggcg tcgccagcgg tccgttccag
     1081 gtctccggcg tggagatcga cagccgcgac gtcgtggaag gggatctgtt cttcgcgctg
     1141 aaaggcgaga cgatggacgg ccaccgcttc ctcgaaggcg ctttcgccaa aggcgcggcg
     1201 gcggcggtgg tcgatcggaa aatccccggc aaccatatcc ttgtcgcaga tacctcggtc
     1261 gccctcgaac ggctcgccca ggccgcgcgc gcgcgcatcg cttccaccag cgtcgtgctc
     1321 ggcgtcaccg gctcggtcgg caagaccagc gtcaaggaag ccctcttcgc cgcgctcgac
     1381 cgcgccagcc atggccgggc ccaccgctcg gtcaagagct acaacaacca cgtcggcgtg
     1441 ccgctcagcc tctcgcgcat gcccgcgggc acccagttcg gcgtgttcga gatgggcatg
     1501 aaccacgccg gcgagatcga cggcctgacc cggcaggtcc gcccccacat cgccctgatc
     1561 accaccatcg cccccgccca catcgagaac ctcggctccg aggaagccat cgccgatgcc
     1621 aaggccgaga tcttccgcgg gctcgagccg ggcggcacgg ccatcatccc ggccgacagc
     1681 ccccatttcg cccgcctgcg cgccgccgcc ctgctcctcc aggccgaggt tgtctccttc
     1741 ggccgcgcgg atcatgccga cgtgcgcctg ctcgatgccg tccccgcgcc cggcggcggc
     1801 tcgctcgtca cgatcgacat gggcgaccgc cgcctgtgct acaccgtcgc cgcgcccggc
     1861 gagcactggg tgacgaactc gctcgccgtc atggcggctg tccgcgcggc gggcggcgat
     1921 ctcggcgcgg ccggcctcgc gctcggcgaa ctcgccggca tgaagggcag gggggcccgc
     1981 cacgagctgc ccacgcccga cgccggcacg gcgctcctga tcgacgaaag ctacaacgcc
     2041 aaccccgcct cgatgaccgc caccctggcc cagctcggcg ccaccccggc catccgccgc
     2101 atcgccgtcc tcggcgcgat gaaggagctg ggcgaacacg gccccgcgat gcacgccgac
     2161 ctcgccggcc ccctcgccgc ggcccaggtc gatcacgcga tcctcgtcgg cgacgagatg
     2221 ggcgccctgg ccgacgaact ggggaaatcg gccggccccg cgcttggcaa aaccgtgcag
     2281 ttctcccatt gcccggacac cgccgcggcg atcgccgccc tggcggacct cggcctggca
     2341 agcggcgacg cgatcctggt caagggctcg aattcggtgg ggctcggcaa gctggtcgcc
     2401 gccctcctcg acgacaaagg ttaggtccgc gaagacatgc tctacctgct cgccgaatgg
     2461 atgcacttcc ccggcctggc gaacctgatc cgctaccagt cgttccgcgc cggcgcggcg
     2521 ctgatcacgg cgatgctcat cggcctcgtc atcggcccgc gcttcatcaa catgctgcgc
     2581 gtacgccagg gcaagggcca gccgatccgc gaggacggcc ccgccaccca cctcgccaag
     2641 cgcggcacgc cgaccatggg cggcctgatg atcctgatca gcctggtcat ctcgatgctg
     2701 ctgtggatgg acctgacgaa ccccttcgtc tgggcctgcc tcgccgtcac ggccggcttc
     2761 ggcgtgatcg ggttcatgga cgatttcgac aaggtcacga aatacagcca caagggcgtc
     2821 cccggccgtg tgcggctggc gatggagttc gtggtggccg gcatcgccgc ctggatcatc
     2881 gtcagccaga tcagcaccaa tctctacgtg cccttcctca gcggccgcta catcccgctc
     2941 g
//
LOCUS       DATNBV010000057         3872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2906397,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000057 DATNBV010000000
VERSION     DATNBV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2906397"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VN627_01340"
     CDS             complement(<1..760)
                     /locus_tag="VN627_01340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD01927.1"
                     /translation="MGMNTEELEMLRDSLRALLDEIAPPKKVREWDKADHVPPELIRR
                     FGEFGVTALAVDEEYGGVGRDVPALLVVIQEVAKRSMAMCSLVIHCTNYAGLNISEQG
                     SEAQKRELLPRVARGELLFALGLSEPNVGADLPSVETRAELRGDKVIINGAKRWCTGA
                     EISDYVYALVRSGPVEDRYKNLSFVLIPTDAPGVSMTSTGTMGIRGIPTNDVVFEDVE
                     VPVENILGGPEFWNKGWSQLAGPTLEVEKLSPSTM"
     gene            complement(905..3001)
                     /locus_tag="VN627_01345"
     CDS             complement(905..3001)
                     /locus_tag="VN627_01345"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HXD01928.1"
                     /translation="MSGYVEKSGLRVATELAGFVEDRVLPGTGIAADAFWAGAADIFA
                     RFVPRNRALLAKRDALQAAIDDWHLARAGQPHDPQAYRTFLTELGYLVPEPAPFTVNP
                     VNVDRELAETAGPQLVVPVLNARFLLNAANARWGSLYDALYGTDAIPGAAAGKGYDPA
                     RGAQVIAWAKAFLDTAAPLAAGTWAEWTGGDLALADPAQLIGRAGDNLLLAHNGLHIE
                     VVIDRAHPIGRDDPAGIADVVMESALTTICDFEDSVAAVDAEDKVLAYGNWLGLMQGN
                     LEDTFEKGGAMITRRLNPDRAYTAPDGTAFTLPGRSLLLVRNVGHLMTTPAVLLADGS
                     EAPEGILDGIVSTTIALHDLRGARANSRTGSVYIVKPKMHGPEEAAFANDLFDAIEDM
                     VGLDRNTLKIGVMDEERRTSANLAACIHAVRNRLMFINTGFLDRTGDEMHTSMQAGPM
                     LRKGDMKTTAWITAYEDRNVQIGLACGLSGKAQIGKGMWAMPDRMADMLEQKIGHPRT
                     GANTAWVPSPTAATLHATHYHRVDVFARQAERRSEPVASLDALLSIPVAGGRNWSAEE
                     IREEIDNNAQGILGYVVRWIDQGVGCSKVPDINDVGLMEDRETLRISSQHLANWLLHG
                     VVSAEQVDAALLRMAGKVDAQNAGDPLYQPMASDPAGSLAFQAARALVFEGVAQPNGY
                     TEPLLHKFRARVKAAA"
     gene            3094..>3872
                     /locus_tag="VN627_01350"
     CDS             3094..>3872
                     /locus_tag="VN627_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXD01929.1"
                     /translation="MFDPDYDLFLDIVAAGSISGAARSRAISVASLSKRLARLEQRLG
                     VRLAHRTTRRLALTQAGKDLVDTLLPMRAELGAAEERLAGRKTAAGGPLRLTAPTSFG
                     RMHVLPCLPGFLAAHPEVELSIDLSDQFVDLLDGGYDLAIRISARIGAGLTAHRLATS
                     GRVLCAAPGYLARFGTPATLRDLAGHHLLAADGQLPWPLEGPDGAILHAGASHVRTNS
                     SEVVRELAIGGCGIALRSLWDVADELETGALVRVLPQYAGS"
BASE COUNT          592 a         1364 c         1253 g          663 t
ORIGIN      
        1 ccatcgtgct cgggctgagc ttttccacct ccagcgtagg tccggccagc tgcgaccagc
       61 ccttgttcca gaattccggc ccgcccagga tgttctcgac cggaacctcc acgtcctcga
      121 aaaccacgtc gttcgtgggg atgccgcgaa tgcccatcgt gcccgtcgac gtcatcgata
      181 ccccgggcgc atcggtgggg atgagcacga acgacaggtt cttgtaccgg tcctccaccg
      241 gcccgctgcg aaccagggca tagacgtaat cgctgatttc ggcaccggtg caccagcgct
      301 tggcgccatt gatgatgacc ttgtcgccgc gcagttcggc ccgggtttcg acagagggca
      361 ggtcggcgcc gacattgggt tcggacaggc ccagcgcgaa cagcagctcg ccccgggcga
      421 cgcggggcag gagttcgcgc ttctgcgctt cggagccctg ctcgctgatg ttgagcccgg
      481 cgtaattcgt gcaatggata accagtgagc acatcgccat cgagcgcttg gccacttcct
      541 ggatgacgac cagcaaggcc ggcacgtcgc ggcctacccc gccgtattcc tcgtccaccg
      601 ccagggcggt cacgccgaat tcgccgaagc ggcggatcag ttcgggcgga acgtgatcgg
      661 ccttgtccca ctcgcgcacc ttcttgggcg gggcgatctc gtccagcagg gcgcgcaggc
      721 tgtcgcgcag catctccagc tcttcggtgt tcatccccat ggccgcctca ctccctcgaa
      781 catctctttc gttcggcagg tgccagactt gtgcggcatg tcaaaaagga tcagcccatg
      841 cacggggcga aagcacaact gtgctgtgct ttcgccaatt ccatgcacct gccgggcagg
      901 agaattaggc cgctgctttg accctggcgc ggaacttgtg cagcaggggt tcggtatagc
      961 cgttgggctg ggcaacgcct tcgaacacca gcgcccgcgc tgcctggaag gccaggctcc
     1021 cggcgggatc gcttgccatc ggttggtaca gcggatcccc ggcattctgc gcatcgacct
     1081 taccggccat gcgcaacagg gctgcatcga cctgctcggc gctcacaacc ccatgcagca
     1141 gccagttggc aaggtgctgg ctggaaatcc gcagcgtctc gcggtcctcc atcaggccca
     1201 cgtcgttgat gtcgggcacc ttcgagcagc cgaccccctg atcgatccat cgcaccacat
     1261 agccaaggat gccctgcgcg ttgttgtcga tctcctcgcg gatctcctcg gccgaccagt
     1321 tgcgccctcc ggcaaccggg atcgacagca gagcgtcgag cgaagccact ggctcgctgc
     1381 ggcgttcggc ctggcgggca aagacatcga cgcggtgata atgcgtcgcg tgcagcgtcg
     1441 ccgcggtagg cgacggcacc caggcggtgt tggcgcccgt gcgcgggtgg ccgatcttct
     1501 gctcgagcat gtcggccatg cggtcgggca tggcccacat gcccttgccg atctgcgcct
     1561 tgcccgaaag tccgcaggcc agaccgatct ggacgttgcg gtcttcgtag gcggtgatcc
     1621 aggcggtcgt cttcatgtcg cccttgcgca gcatgggccc ggcctgcatc gaggtgtgca
     1681 tctcgtcgcc cgtgcggtcg aggaagccgg tgttgatgaa catgaggcgg ttgcgcacgg
     1741 cgtggatgca ggcggccagg ttggcgctgg tccggcgctc ctcgtccatc accccgatct
     1801 tgagcgtgtt gcggtcaagg ccgaccatgt cctcgatcgc gtcgaacagg tcgttggcga
     1861 aggccgcttc ctcgggcccg tgcatcttcg gcttgacgat gtagaccgaa cccgtgcggc
     1921 tgttggcgcg cgcgccgcga agatcgtgca gcgcgatcgt ggtcgagacg atgccgtcaa
     1981 gaatgccctc gggcgcctcg ctgccatcgg ccagcaacac cgccggcgtg gtcatcagat
     2041 ggccgacatt gcgcaccagc agcagcgagc gccccggcag ggtgaatgcc gtgccgtcag
     2101 gcgcggtata ggcgcggtca ggattcaggc ggcgggtaat catggccccg cccttctcga
     2161 aggtgtcctc gaggttgccc tgcatcaggc ccagccagtt gccataggcc agcaccttgt
     2221 cctcggcatc gaccgcggcg accgaatcct cgaagtcgca aatcgtcgtc aacgccgatt
     2281 ccatcaccac gtcggcgatg ccggcgggat cgtcccggcc gatcggatgg gcgcggtcga
     2341 tcaccacctc gatgtgaagg ccgttgtgcg cgagcaagag gttatcgccg gcccggccga
     2401 tcagctgcgc cggatcggcc agggcaaggt cgccgccggt ccactcggcc caggtgcccg
     2461 ccgcgagcgg ggctgccgtg tcaaggaagg ccttcgccca ggcgatgacc tgcgcgccgc
     2521 gcgccgggtc atagcccttg ccggcggccg cgccggggat cgcatccgta ccatagagcg
     2581 cgtcgtagag gctcccccac cgcgcattgg cggcattgag caggaaccgg gcattgagca
     2641 ccggcactac cagctgcggc ccggcggttt cggcgagctc gcggtccaca ttgaccgggt
     2701 tgaccgtgaa cggcgccggt tcaggaacca gatagcccag ctcggtcagg aaggtgcggt
     2761 aggcttgcgg atcatgcggc tggccggcgc gggcaaggtg ccagtcgtcg atcgccgcct
     2821 gcagcgcgtc gcgcttggcc agcagcgcgc ggttgcgcgg gacgaaccgc gcgaagatgt
     2881 cggcagcgcc ggcccagaat gcgtccgccg cgatgcccgt gcccggcagc acacggtcct
     2941 caacgaaccc ggccaactcg gtcgccaccc gcaaacccga cttctcgaca taaccgctca
     3001 tgtgtgcctc gctttctgcc gcttccccta gccgagcccg catccatgca gtagatggct
     3061 ataattcaac acagcttctc cctcagggaa acaatgttcg atccggacta cgatctgttc
     3121 ctcgatatcg tcgccgccgg cagcatttcc ggggcggcgc gcagccgcgc catctcggtg
     3181 gccagcctgt cgaagcggct ggcccggctc gagcagcgcc tcggcgtccg gctggcccac
     3241 cgcaccaccc gccgcctcgc cctgacccag gcgggcaagg acctggtcga cacgctcctg
     3301 ccgatgcggg ccgagcttgg cgcggccgag gagcggctgg cgggccgcaa gaccgccgcc
     3361 ggcggccccc tgcgcctgac agcgcccacc tcgttcgggc gaatgcacgt actgccctgc
     3421 ctccccggct tcctcgccgc ccaccccgag gtggagctgt ccatcgacct ctccgaccag
     3481 ttcgtcgacc tgctggacgg cggctatgac cttgcgatcc gcatcagcgc ccggatcggc
     3541 gccggcctca cggcgcatcg gctggccacc agcggccgcg tcctctgcgc ggcgcccggc
     3601 tacctcgccc ggttcggcac gccggccacc ttgcgcgacc ttgccggcca ccatctgctt
     3661 gccgccgatg gacagctgcc ctggccgctc gaagggcccg atggcgctat tctccatgcc
     3721 ggcgccagcc acgtccgcac caattccagc gaggtcgttc gcgagctggc gatcggcggc
     3781 tgcggcatcg ccctgcgctc cctgtgggat gtcgccgacg agctggagac cggcgcgctg
     3841 gtccgggttc tcccgcaata cgcgggatcg ca
//
LOCUS       DATNBV010000058         3989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3867156,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000058 DATNBV010000000
VERSION     DATNBV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3867156"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="VN627_01355"
     CDS             <1..1399
                     /locus_tag="VN627_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HXD01930.1"
                     /translation="IATGAGDDLVINGPGAATVDGGPGADVFQVGGLKAAYAITRDGS
                     GGYFLTEAATGTQDRLTAVEQVRFADAVADLPFYALGVTLTGTAGADTLTGTPWADRL
                     SGLAGNDTLVAGDGDDWLSGGPGDDRLTGGPGIDTLDYADAPALVKVNLAITGKAQAT
                     GGAGNDWLVDLFENVTGSAFADTLTGNALANRIEGAAGADIIDGLAGADTLVGGLGND
                     IYVVDDAADVVIENAGEGTDLIKSAISFALPGNVENLTLTGTLAVDGAGNAQGNSLVG
                     NAAANHLSGGDGNDTLDGAVGADTLAGGLGNDSYKVDNTGDAVIEGAAEGTDLVTAAV
                     TFTLSANVENLTLSGTLAIDGTGNDLANSLIGNAAANHLYGLGGNDTIDGGPGADSMA
                     GGPGNDTYKVDDPGDSIVENPGEGTDLQRHFHPRRQYREPHSHRLNRDQRHGQCLGQF
                     PHWQRRGQRARRARW"
     gene            1377..1796
                     /locus_tag="VN627_01360"
     CDS             1377..1796
                     /locus_tag="VN627_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01931.1"
                     /translation="MLVGLAGDDTLNGGGGDDTLSGGPGADTLTGGLGTDLFVFDQRE
                     TAANRDAIQDFAHLTDRMAFDRAAFTAFANLTAGALDPTQLNLGTTATTAQHHLVYTQ
                     STGSLYYDTDGAGGAAQVLVAVLSTKPVLSAADIILL"
     gene            1929..2894
                     /locus_tag="VN627_01365"
     CDS             1929..2894
                     /locus_tag="VN627_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HXD01932.1"
                     /translation="MARPAILITAPMHHSVEAALAPHFALYRLWEQRDPDAFLRTNAH
                     DIRGVATTTLHGRIDEAFYALFPAIEIVASFGVGYDNVDVSAAAARGIVVTNTPGVLD
                     DEVADLTIGLLIATLRQIPQAERYLRAGSWLDAPFPLSHSLRGRTVGILGLGGIGKAI
                     ARRLEGFDVAIAYHGRTQQEGVRYPWLPTPLALAQACDTLIAILPGGAATRHIVDSPV
                     LAALGPEGVFINVARGSVVDQTALITALRDGTILAAGLDVYADEPHVPAELVELPNAV
                     LLPHIASASEHTRTAMGCLVADNLIAWFGEGRPLTPVAETPFPRS"
     gene            complement(2891..3655)
                     /locus_tag="VN627_01370"
     CDS             complement(2891..3655)
                     /locus_tag="VN627_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HXD01933.1"
                     /translation="MGDSAQIAPPVVANDLAFADLWAALAAGWHDFMACPIVGLALAA
                     IYVAGGWLLYFAFYRMGQVAWLVPAAGGFPIIAPLVAVGLYEASRTREAGGRPGWGTV
                     AAGVRGRGDDQVLMLAGFLFVGVTFWIIIAHGIFAIFLSESGIGTESLALFATPAGIA
                     MLAVGGAAGALLAFLFYAVTVVSLPLLIDREVDFLTAMMVSMAAVRNNFAVMLGWGLI
                     IAVGLGLAMLPLFLGLFVVLPVLGHATWHIYRRVIR"
     gene            complement(3730..>3989)
                     /locus_tag="VN627_01375"
     CDS             complement(3730..>3989)
                     /locus_tag="VN627_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD01934.1"
                     /translation="IGWAVGRRPWVLHMSQPMLGRIARFDGWWRKDRAKLTADRVSYM
                     CHPDWVVSERARPPARLWQPQIETRSGLEATAKWYREQGWL"
BASE COUNT          716 a         1413 c         1219 g          641 t
ORIGIN      
        1 catcgcgacc ggggcgggcg acgatctggt catcaacggt cccggcgcag caacggtcga
       61 cggcggtccc ggggcagatg tgttccaagt cggcggcctc aaggccgcct atgccattac
      121 ccgcgacggc agcgggggct atttcctgac ggaagcagcc actggaacgc aggaccggct
      181 gaccgctgtc gaacaggttc gcttcgccga tgccgtggcc gacctgccgt tctacgcgct
      241 cggggtaacc ctcaccggaa cggcaggcgc cgatacgctc actggcaccc cttgggctga
      301 ccgcctgtcg ggcctcgcgg ggaacgacac gctggttgcc ggtgatggcg acgattggct
      361 ctctggcggc ccgggcgatg atcggctgac gggcggcccc gggatcgata cgcttgacta
      421 tgccgatgct cccgcgctgg tgaaggtcaa ccttgccatc acaggcaagg cccaagccac
      481 gggcggagca gggaatgact ggctggttga cctgttcgaa aacgtcaccg gctcggcctt
      541 tgccgatacc ctgaccggca atgccctggc caatcgcatc gagggcgccg ccggggcgga
      601 tatcatcgat ggcctggccg gggccgatac cctcgtcggc gggctcggca acgatatcta
      661 cgtcgtcgac gatgcggctg acgtggtgat cgaaaatgct ggcgagggca ccgatctgat
      721 caagtcggcc atatccttcg ccctgcccgg caatgtcgag aacctcaccc tcaccggcac
      781 cctggccgtg gacggcgccg gcaacgctca gggcaattcc ctcgtcggca acgccgcggc
      841 gaaccacctc tccggcggtg acggcaacga cacgctggat ggcgctgtcg gcgccgatac
      901 gcttgctggt ggcctcggca acgacagtta caaagtcgac aataccggtg atgccgtgat
      961 cgaaggagct gccgaaggca ccgacctcgt caccgctgcc gtcactttca ccctctccgc
     1021 taatgtcgag aatcttaccc tgtccggcac cctcgcgatc gatgggaccg gcaacgatct
     1081 ggccaacagc ctgattggca atgccgccgc caaccatctc tatggcctcg gtgggaatga
     1141 caccattgat ggtggccccg gggccgacag catggcgggc ggccccggca acgacacgta
     1201 caaggttgac gatccgggtg acagcatcgt ggaaaacccc ggcgaaggca ccgatctgca
     1261 gcgccacttt caccctcgcc gccaatatcg agaacctcac tctcaccggc tcaatcgcga
     1321 tcagcggcac gggcaatgcc tcggccaatt ccctcattgg caacggcgcg gccaacgtgc
     1381 tcgtcgggct cgctggtgac gatacgctga atggcggcgg gggcgatgat accctctccg
     1441 gtggccccgg cgccgatacg ctcacaggcg ggctgggcac ggacctgttc gtgtttgacc
     1501 agcgcgagac cgccgcgaac cgcgatgcga tccaggactt cgcccacctg accgaccgga
     1561 tggcgttcga tcgtgcggcg tttactgctt tcgccaacct cacggcgggc gcactggatc
     1621 ccacccaact taacctcggc accaccgcca cgacggccca gcaccacctc gtgtacacgc
     1681 agtccaccgg ctcgctctat tacgatacgg atggcgcggg aggcgcggcc caggtgcttg
     1741 tggccgtgct ctccaccaag ccggtgctct cggcggccga catcatcctg ctttagcccc
     1801 acgggtgaga ggcagcggcg aggggctgaa cgacgggcgt gcctcttatc gcggcgccga
     1861 cctggtgatg cggccgacag gcactgctgc ggctggctcc gcacgcatgc cccggctatc
     1921 gtccgtcgat ggcccgcccc gcgatcctga tcaccgcgcc gatgcaccat tcggtggaag
     1981 ctgccctcgc cccgcatttc gcgctttatc gcctgtggga acagcgcgac cccgacgcgt
     2041 ttctccgcac aaatgcccat gatatccgcg gcgtggccac caccaccctg catggcagga
     2101 tcgatgaggc gttttatgcc ctgttcccgg cgatcgagat cgtcgcctcg ttcggtgtcg
     2161 gttacgacaa tgtcgatgtc agcgccgccg cggcgcgcgg cattgtcgtc accaacacgc
     2221 ccggcgtgct tgacgacgag gtggcggatc tcaccatcgg cttgctcatc gccaccctgc
     2281 gccagatacc ccaggccgaa cgctacctgc gggcaggaag ctggctggat gcgccgtttc
     2341 cgctttccca tagcctgcgg ggccggacag tcggcatcct cggcctcggc ggcatcggca
     2401 aggcgattgc ccggaggctt gaaggcttcg atgtggccat cgcgtaccat gggcgcacgc
     2461 agcaggaggg cgtgcgctac ccctggcttc ccactccgct cgccttggcc caagcatgcg
     2521 acacgctgat cgccatcctt cccggcggcg cggccacgcg ccacattgtg gattcgccgg
     2581 ttctcgccgc gctcggcccc gagggagtat tcatcaatgt cgcgcggggc agcgtggttg
     2641 accaaacagc actgatcacc gcacttcggg atggcacgat cctcgccgcc ggactggatg
     2701 tctatgccga tgagccccat gtgccggcgg agctggtcga actgcccaac gctgtcctgc
     2761 tgccccacat cgcctcggcc tcggaacaca cccgcactgc gatgggctgc ctggttgccg
     2821 acaacctcat cgcctggttc ggcgagggcc gcccgctcac cccggtggcc gaaacgccat
     2881 ttccgcgttc ctagcgaatc actcgccggt aaatatgcca ggtggcatgt cccagcaccg
     2941 gcaagaccac gaacaggccg agaaacagcg gcagcatggc aagccccagt cccacggcga
     3001 tgatcagacc ccacccgagc attaccgcga agttgttcct gacagcagcc atgctcacca
     3061 tcatcgccgt caggaaatcc acctcgcggt cgatcaggag aggcaggctt actacggtca
     3121 ccgcatagaa cagaaaggcg agcaacgcgc ccgccgcccc gccgactgcc agcatggcga
     3181 tccccgccgg ggtggcgaac agagcaaggg attcggtgcc aatcccgctt tccgacagga
     3241 aaatggcgaa aattccgtgg gcgatgatga tccagaacgt cacgcccacg aacaggaacc
     3301 cggccagcat cagaacctgg tcatcccccc gcccgcgcac tcccgcagca accgtgcccc
     3361 agcccggacg gcccccggcc tctcgggtgc ggctggcctc gtaaagcccc accgccacca
     3421 gcggcgcaat gatcggaaag ccgccggcgg cagggaccag ccaggcaact tgtcccatgc
     3481 gatagaacgc gaaatacagc agccagccgc cggccacgta gatggcagcc aaggccagcc
     3541 cgacgatcgg acaagccatg aagtcgtgcc atcccgccgc gagcgccgcc cacaggtccg
     3601 caaatgcaag atcgttggca accaccggtg gcgcgatctg agcagaatcg cccatcgccc
     3661 tctcctctcc gtcggcttcc cgatgggaag gtgacgcaag taggacggcg catcaagtat
     3721 ggtgaatgcc tagagccagc cctgttcgcg gtaccacttt gccgtggctt ccaaccccga
     3781 ccgtgtttcg atctgcggtt gccacagccg ggcaggaggc cgggcgcgct cgctcaccac
     3841 ccagtcgggg tggcacatgt agctcacccg gtccgccgtc agcttggccc ggtccttgcg
     3901 ccaccatccg tcgaacctgg caatccgccc cagcatcggt tgcgacatat gcagcaccca
     3961 cgggcggcgc cccacggccc agccgatcg
//
LOCUS       DATNBV010000059         2600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1002828,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000059 DATNBV010000000
VERSION     DATNBV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002828"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VN627_01380"
     CDS             complement(<1..858)
                     /locus_tag="VN627_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXD01935.1"
                     /translation="MTTIAELLAGVPAERPAFVAPGEPVLSHGELRRLCRETGAWLNQ
                     RSIGRNDRVAIVLPNGPAMATAFVAIAQAATTAPLNPAYREDEFDFYLGDLAARALIV
                     AAGAETPARAVAARRGIPVLELTPQPDAAGRFTLSGDGPGSGGGQQGPAAADDVALVL
                     HTSGTTARPKMVPLTQANLVASARHIGATLGLTPSDRCLNIMPLFHIHGLIGCVTASL
                     AAGASVFCSPGFNALKFFGWLAEAGASWYSAVPTMHQAILARAERNRDAMAGHPLRFI
                     RSSSASLPPQ"
     gene            1044..2282
                     /locus_tag="VN627_01385"
     CDS             1044..2282
                     /locus_tag="VN627_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXD01936.1"
                     /translation="MVDLDSQLSADHRARAVWTFVSGLDLSEFYARIKARGEVAGRPA
                     TDPKILLALWLYATLEGVGSARALDRLCRHHAVYRWLCGGVEVNHNILAEFRVESGAL
                     VDRLLTQSLTALIAAGLVRLEEVAIDGTKVRAAASKGSLSGGGKLARLSAAVGARVQA
                     LKDELASDPESSERRVKKRALQAAEEQARRVAAAAAKLAELEQERQERERTHPAEEAD
                     KGEPKASTSDVEARTMRMADGATRLAYNVQVATACGFVIAIEPTDRRNDKGLAPMLVG
                     QIEARCQAVPGRLLADSGSMTCADIASLAGAHPEMVVYSPPPAERDDVTEETKRKRAW
                     KRRREAPAVLRWRERMADAQAKLIYARRKNTEHAHAHLKNRGFGHMVVRGLAKVRAVS
                     LLHALAHNLCRALTLRAATP"
BASE COUNT          406 a          834 c          978 g          382 t
ORIGIN      
        1 ctgcggcggc agcgaggcgg aggaggagcg gatgaagcgc agcggatgac cggccatggc
       61 gtcgcggttg cgctcggcgc gggcaaggat cgcctggtgc atggtcggca ccgccgaata
      121 ccagctggcg ccggcctcgg ccagccagcc gaagaacttc agcgcgttga agcccgggct
      181 gcagaacacg ctggcgccgg cggcgagcga ggcggtgacg cagccgatca gcccgtggat
      241 gtggaacagc ggcatgatgt tgaggcagcg gtcggaaggc gtcagcccca gcgtcgcgcc
      301 gatgtggcgc gccgaggcca cgaggttggc ctgggtcagc ggcaccatct tgggccgcgc
      361 cgtggtgccc gaggtgtgca gcaccagcgc gacgtcgtct gccgcggccg gcccttgctg
      421 cccgccgccg ctcccaggcc cgtcgccgga cagcgtgaag cggccggccg cgtcgggctg
      481 tggcgtcagc tcgaggaccg ggatcccgcg ccgcgccgcc acggcgcggg ccggagtctc
      541 cgctccggcg gcgacgatca gggcgcgggc ggcgagatcg ccgagataga agtcgaactc
      601 gtcctcgcga taggccgggt tgagcggcgc cgtggtcgcc gcctgggcga tcgccacgaa
      661 ggccgtcgcc atcgccggcc cgttcggcag cacgatcgcc acccggtcat tgcgcccgat
      721 ggaccgttga ttgagccagg cgccggtctc gcggcagagc cgccgcagct cgccatggct
      781 cagcaccggc tcgccgggcg ccacgaaggc cgggcgctcg gcggggacgc cggccagaag
      841 ctctgcgatg gttgtcatca ggaacgtcct ctacgtcacc aacacgtcat cccggcgaaa
      901 gggcctgtaa ttttttgttg gttggtgagt ttggggttcc ctggtgatgg ataggatgat
      961 tcgatgggcg tgctgccagg gaggattgga tgagtgatga ggccagggtg aagcaggctg
     1021 atcgtcggca gcttcggtgg gacatggtgg atctcgacag ccagctttcg gcggatcacc
     1081 gggctcgggc ggtgtggacg tttgtgtcgg gattggacct gagcgagttc tacgctcgga
     1141 tcaaggcgcg tggtgaggtg gcgggtcgtc cggcgacgga cccgaagatc ctgctggctt
     1201 tgtggcttta cgcgacactg gagggtgttg gctcggcgcg ggctttggat cggctttgcc
     1261 ggcatcacgc ggtgtaccgg tggctgtgcg gcggggtcga ggtcaatcac aacattctgg
     1321 ccgagttccg ggtcgagagc ggggcgcttg tggaccgtct gctgacgcag agcctgacgg
     1381 cgctgattgc tgccgggctt gtccggctgg aggaagtggc gatcgacggg accaaggtga
     1441 gggcggcagc gagcaagggc tcgctttcgg gtgggggcaa gctggcgcgc ctgtcggcgg
     1501 cggtaggtgc ccgggttcag gcgctgaagg acgagctggc ctcggatcct gaaagctcgg
     1561 agcggcgggt caagaagcgg gctttgcagg cggccgagga gcaggctcgc cgggtggcgg
     1621 cggcggcggc gaagctggcc gagctcgagc aggagcgcca ggagcgcgag cggacgcatc
     1681 cggccgaaga agctgacaaa ggcgagccga aggcctcgac gagcgatgtc gaggcgcgga
     1741 cgatgcggat ggccgacggc gcgacgcgcc tggcctacaa cgtccaggtg gcgacggcgt
     1801 gtggcttcgt gatcgcgatc gagccgaccg accggcgcaa cgacaaaggg ctggcgccga
     1861 tgctggtcgg gcagatcgaa gcccgctgcc aggcggtgcc cggtcgtctg ctggccgata
     1921 gcggatcgat gacctgcgcc gacatcgcca gcttggccgg cgcgcatccc gagatggtgg
     1981 tctatagtcc gccgccggcc gagcgcgacg acgtgacgga ggagaccaag cgcaagcgcg
     2041 cctggaagcg ccggcgcgag gcgcccgccg tgctccgctg gcgcgagcgc atggccgatg
     2101 cgcaggccaa gttgatctat gcccggcgca agaacaccga gcatgcccat gcccacctga
     2161 agaaccgcgg cttcggccac atggtggttc gcggcctcgc caaggtacgg gccgtttccc
     2221 tgctgcacgc gctggcccac aatctttgcc gcgccctgac cctgcgcgcc gccacgccgt
     2281 gagcccaact cacccaatcg ccagccgcca aaccctcgcc ggcccgcggg ccagccaaac
     2341 atcgcctcga aacccggcac cacccgttgg ccgaccatcc gcagtaatgt cccgccaccg
     2401 aaacccaatt tcgttacggg ccctttcgcc gggatgacgg cgagcggcaa tgccgctacc
     2461 gcgccagctc gcggccccgc tgggcagcta cttcgtgacc gcccggtaga acaccaggtc
     2521 gctcgtcgga ccggcgacga agcccttgac gttggcccgg ctggccagcg gctcggcctg
     2581 ctggaacagg acgatcaccg
//
LOCUS       DATNBV010000060         6998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2272173,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000060 DATNBV010000000
VERSION     DATNBV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272173"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1834)
                     /locus_tag="VN627_01390"
     CDS             complement(113..1834)
                     /locus_tag="VN627_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXD01937.1"
                     /translation="MALRFPLGSFSRLPGLGQQTIAVRCRALGLVLIAIALAAVTVSL
                     GIGNEIRIGGRLHAQERDYADLVADILPPPEYIIEPWLETTLIAQGEGSAADHLERLA
                     ALRRDYETRKAYWAAKDLPADLADSMRRANRQADAFWKVADDEFAPAVRAADRTRILA
                     VHARLGTIYQAHRAEIDRLVTQANAATASLTDRSRHLVMVALAIIGGMAVLLIGAIAA
                     GVAMLLRKGLAPLTETARVLGEGLDQLASGRIDHRITEPLPAEYLPLRNSFNATAEAL
                     SGLITALDGAATNIGSAGAQIREASTDLAERAEREAHGIHTAAQVIKDVTAMSGECWR
                     SVEQVNAAVATTRSEAVEARGIVCATMSTMERIERSSKEIAKIADTVDGIAFQTNLLA
                     LNAGVEAARAGDAGKGFAVVAAEVRALAQRTTTAAQEIGRLLADSDQEVSLGVRNAAS
                     SEASFQAITAHVEQVSVMLGKVVDLARHQSSRLEEVNGSVREIEELTQHNAAMAEECS
                     AASRAMTSESSTLLEIVDGFSQSRRGHTRQASGQQSDNGAGRALPRPGQAAPRPRLSA
                     PGEPLAA"
     gene            complement(1964..4012)
                     /locus_tag="VN627_01395"
     CDS             complement(1964..4012)
                     /locus_tag="VN627_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXD01938.1"
                     /translation="MSSMVRKSLAGAFLVFLATEGPASSQSVVSVPVVQPVPQSSPTP
                     VWQGQTFVPTSGEGSEWDRARAQLRLSAATPMAQAVERWKLLASTDNLAFADYSGFLV
                     AYPGFPEEDKLRRAAEKALDRGGAQPAQVAAYFDRVAPLTNPARAQYALALAALGRTG
                     APAWALAAWRGGAMSDAAEAALAPLVRAGPADQDARMDALLWAGATTQAQRQLALVSP
                     ARQPLFAARLAALQGNDPTALGLVLPADASADPGWLYNRARQLLNAGRSAEAAFLLAN
                     RGRLAALPLDAEKWVALQLAAARSADAATAVRLAATIDDAFAPGTEISRASFRLRDDY
                     TSLMWLAGTRAVWSLGAPRSAAPLFYRYGMAAKTPQTRAKGLYWAGRALAQGGDSAAA
                     TQYFTQAARYADQFYGQLALERLGKPMPDFAKAPSVTPTLAQRQAFLSRPIAGAVREV
                     ARESDWPTAVRFFKEIANQAETPAEHQIVADLARELGRRDLGVILGQAAGAEGIQDFQ
                     HVAFPLIPTPEGADWTMVHAISRQESQFAQNAVSHAGARGLMQLMPRTAAQEAGRVGL
                     AFDAGALMSQPGYNLKIGDTFFRRMLNYYGGSYPLAIAAYNAGPGNVNKWLRANGDPR
                     TGAVDWIDWIERIPIQETRNYVQRVSENAVVYEAMNPDKAHYRGANPLSHFIGKSGPG
                     "
     gene            4123..5004
                     /gene="dapA"
                     /locus_tag="VN627_01400"
     CDS             4123..5004
                     /gene="dapA"
                     /locus_tag="VN627_01400"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HXD01939.1"
                     /translation="MFSGSIPALVTPFRDGAFDEKAFRRLVDWQIESGSSALVPCGTT
                     GEASTLSNDEHHRVIEVCIEQAAGRVPVIAGCGSNDTMNALLHMETSQQLGADAALVV
                     APYYNRPSQEGMLAHFSYLAEHSDLPIVLYNVPGRTVTDLKPATVIELARRFPGKIIG
                     MKDASGDPGRVTEHRLGIGAEFCQMSGDDGLALPFLAAGATGCISVTANVAPGLCAKF
                     QAAWAAGDVAGARALNDRLYPLHSAMFCDASPGPVKYAMARVLDWMAGDLRLPMTPCD
                     EAGRKVVDAALVHAGLI"
     gene            5098..5358
                     /locus_tag="VN627_01405"
     CDS             5098..5358
                     /locus_tag="VN627_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01940.1"
                     /translation="MATRLPSAEDRRVFDAPLHVAHGADGTVTVFFPDGHRMVLTAEA
                     AERSGAILWRTALALRSRRRGGPAHRSGQVIAVDFGEHAPRH"
     gene            5440..5922
                     /gene="smpB"
                     /locus_tag="VN627_01410"
     CDS             5440..5922
                     /gene="smpB"
                     /locus_tag="VN627_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXD01941.1"
                     /translation="MARPTPAQFDKKKIVAENRRARFDYHIDEVFEAGIALTGTEVKS
                     LRFGEGSIAESYAEVKNGEVWLVNSNVPEFSHGNRFNHEPKRLRKLLLKEREINKFVG
                     ATERKGMTLVPLSIYFNSRGRAKVELALAKGKNAADKRQTVKDRDWKREQGRLMREHG
                     "
     gene            6061..6618
                     /locus_tag="VN627_01415"
     CDS             6061..6618
                     /locus_tag="VN627_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HXD01942.1"
                     /translation="MVTKFTSWAKRKMPTREDMERSRFIRPFAHRVLASEVWRFTRRS
                     VPRGVALGMLVGIIVPFAQVLFAAFFSLSVRANVPVAALTTFITNPFTTPFIWAASYK
                     VGERLLYFDGMFHANPIDSLMRVTDGWQLIEWITAEGKVLALGLIVVAVIAAAVSYLA
                     ASFGWNAWVRTKRRRRRQARAAQGA"
     gene            6615..>6998
                     /locus_tag="VN627_01420"
     CDS             6615..>6998
                     /locus_tag="VN627_01420"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HXD01943.1"
                     /translation="MTTRLIGWLRSHVPTREALERNRFVRPFAHLILRSELWRFTRRS
                     VPRGVALGLFVGVMIPLAHFVVAAFLAVFLRANIPAAMLATFIGFPVIYVALVALAYK
                     IGEWLLHLDAMTGIQPLGETMQTTQT"
BASE COUNT         1047 a         2227 c         2586 g         1138 t
ORIGIN      
        1 cactccccca accttcgcca ggccgttgca cggataagct gcggtagccc ttccccatca
       61 agggggaggg tttttcgtgt tggcttgccg ctactcgggg gagggcgggt gatcaggcgg
      121 cgaggggttc gcccggggcg gacaggcgcg gccttggggc ggcttggccg gggcggggca
      181 aggctcggcc cgcgccgttg tccgactgct ggccggaggc ttgtcgggtg tgcccccgcc
      241 gggactggct gaatccgtcg acgatctcga gcagggtgga cgattcgctg gtcattgccc
      301 gcgacgcggc gctgcattct tcggccatgg cggcattgtg ctgggtcagt tcctcgatct
      361 cgcggaccga gccgttgacc tcttcgagcc gcgacgactg gtggcgggca aggtcgacaa
      421 ccttgccgag catcaccgaa acctgttcga cgtgggcggt gatcgcctgg aagctggctt
      481 cgctggaagc ggcgttgcgg acgccgagcg acacttcctg atcgctgtcg gccaggaggc
      541 ggccgatttc ctgcgcggcg gtggtggtgc gctgggccag ggcgcggact tcggcggcga
      601 ccacggcaaa gcccttgccc gcatcgcccg cgcgggcggc ttcgaccccg gcattgagcg
      661 ccagcagatt ggtctggaag gcgatgccgt cgaccgtatc ggcgatcttg gcgatctcct
      721 tggaggaccg ctcgatccgt tccatcgtgc tcatcgtggc acagacaatt ccgcgcgcct
      781 cgacggcttc ggagcgggtg gtcgcaacgg cggcattgac ctgttcgacg ctgcgccagc
      841 attcgccgct catcgcggtg acatccttga taacctgggc ggcggtgtgg atgccatgcg
      901 cttcgcgctc ggcgcgttcg gcgaggtcgg tagaggcttc gcggatctgg gcgccggccg
      961 aaccgatgtt ggtggcggcg ccgtcgagcg cggtgatcag cccgctgaga gcctcggccg
     1021 tggcattgaa ggaattgcgc agcggcaggt attcggccgg caggggttcg gtgatgcggt
     1081 gatcgatccg gccgctggcc agctggtcaa gcccctcgcc gagcacccgg gccgtctcgg
     1141 tcaagggcgc caggcccttg cgcagcagca tggcgacgcc cgcggcgatc gcgccgatca
     1201 acaggacggc catgccgccg atgatcgcca gcgcgaccat caccagatgc ctgctgcgat
     1261 cggtcaagct ggcggtggcg gcattggcct gggttaccag ccggtcgatc tcggcacggt
     1321 gggcctggta gatcgtgccc agccgggcat ggaccgccag gatacgcgtc cggtccgcgg
     1381 cgcgcacggc cggggcgaat tcgtcgtcgg cgaccttcca gaaggcgtcg gcttgccgat
     1441 tggcgcggcg catggagtcg gcgagatctg cgggcaggtc cttggcggcc caataggcct
     1501 tgcgggtctc gtaatcgcgg cgcagggcgg ccagccgctc caggtgatcc gcggccgagc
     1561 cctcgccctg ggcgatcagc gtggtctcca gccatggttc gatgatgtat tcgggcggcg
     1621 gcaggatgtc ggccacgagg tcggcgtagt cgcgctcttg cgcatggagc cgcccgccga
     1681 tccggatctc attgccgatg ccgagcgata cggtgaccgc ggccagggca atggcgatca
     1741 ggaccaggcc caatgcgcgg cagcgcacgg cgatcgtctg ttggcccaat ccgggcaggc
     1801 gggagaacga accaaggggg aagcggaggg ccatgcacga tcctgcggcg aaaggtgatg
     1861 cgacgcgcgg gaccctagcg gtgggcaggt caccgttggc atagcaccgc cctaggggga
     1921 accccgcagg cgatccggga ccggcttcag atttgcgccg ggatcagccc ggcccgctct
     1981 ttccgatgaa gtggctgagc ggattggcgc cgcggtagtg ggccttgtcc gggttcatcg
     2041 cctcgtagac gacggcgttt tcggagacgc gctggacgta gttgcgggtt tcctggatcg
     2101 ggatgcgttc gatccagtca atccagtcaa ccgcgccagt gcgcggatcg ccgttggccc
     2161 tcaaccactt gttcacgttg ccggggccgg cgttataggc ggcgatggcc agcgggtagg
     2221 agccgccata gtaattgagc atgcggcgga agaaagtgtc gccgatcttg aggttgtagc
     2281 cgggctggct catcagcgcc ccggcatcga aggctaggcc gacgcggccg gcttcctgcg
     2341 cggcggtgcg cggcatcagc tgcatcaagc cgcgcgcgcc ggcgtggctg acggcgttct
     2401 gggcgaactg gctttcctgc cggctgatcg cgtggaccat ggtccagtcc gccccctcgg
     2461 gcgtggggat cagcgggaag gcgacgtgct ggaaatcctg aatgccttcc gcgccggcgg
     2521 cttgcccgag gatcacgccg agatcgcggc ggccgagttc gcgggcgaga tcggcgacga
     2581 tctggtgttc ggccggggtt tcggcctggt tggcgatttc cttgaagaaa cgcacggcgg
     2641 tcggccagtc gctctcgcgc gcgacttcgc gcacggcacc ggcgatgggg cgcgagagga
     2701 aggcctggcg ctgcgcgagg gtgggggtga ccgacggcgc cttggcgaaa tcgggcatgg
     2761 gcttgccgag gcgctcgagc gcgagctggc catagaactg gtcggcatag cgcgcggcct
     2821 gagtgaaata ctgggtggcg gcggcactgt cgccaccttg cgcgagggcg cggccggccc
     2881 agtagaggcc cttggcgcgg gtctgcgggg tcttcgcggc catcccgtag cggtagaaca
     2941 gcggggcggc gctgcgcggc gcgccgaggc tccacacggc gcgggtgccg gcgagccaca
     3001 tcagcgaggt atagtcgtcg cgcaggcgga acgaggcgcg gctgatctcg gtgccggggg
     3061 cgaaggcgtc gtcgatcgtg gcggcgaggc gcacggcggt ggcggcatcg gcgctgcggg
     3121 cggcggcgag ctggagcgcg acccacttct cggcgtcgag cgggagggcg gcgaggcggc
     3181 cgcgattggc gaggaggaag gccgcctcgg cggagcggcc cgcgttcagc aactgtcgcg
     3241 cgcgattgta gagccagccc ggatcggccg aggcatcggc gggcaggacc aggccgagcg
     3301 cggtgggatc gttgccctgc agcgcggcga ggcgggcggc gaacagcggc tggcgcgcgg
     3361 gcgagacgag ggccagctgg cgctgggcct gggtggtggc gcccgcccag agcagggcgt
     3421 ccatccgcgc gtcctgatcg gccgggccgg cgcggaccag cggggccagc gcggcctcgg
     3481 cggcatcgct catcgccccg ccgcgccagg cggcgagcgc ccaggccggc gcgcccgtcc
     3541 ggcccagggc ggccagcgcg agggcatatt gcgcgcgggc cgggttggtc agcggcgcga
     3601 cgcggtcaaa ataggcggca acctgagcag gctgcgcgcc gccgcgatcg agcgccttct
     3661 cggcggcgcg gcgcagcttg tcttcctcgg ggaagccggg ataggcgacg aggaagccgg
     3721 agtagtcggc aaaggcgaga ttgtcggtgc tggccagcag cttccagcgt tcgaccgcct
     3781 gggccatcgg cgtggccgcg ctgaggcgca gctgggcgcg ggcgcggtcc cactcgctgc
     3841 cttcgccgct ggtcgggacg aaggtctgcc cctgccagac cggcgtgggg gacgactgcg
     3901 gcaccggctg gaccacgggc acggagacaa cggattgcga ggacgcgggc ccctcggtcg
     3961 ccagaaagac caggaaggca ccggcaaggg acttgcggac catgctggac attcgcctct
     4021 cgctccttat caggcgctga atgaattcac ttggctccgc tgtggaagct cccgtcccgt
     4081 tcgggttagc gctacattgg ggcaaaatca gggaagatgt ccatgttttc cggttcgatc
     4141 ccggcactgg taacgccgtt tcgcgacgga gcgtttgacg agaaggcctt ccgccggctg
     4201 gtggactggc agatcgagag cggttcgagc gcgctggtgc cgtgcgggac gacgggcgag
     4261 gcctcgaccc tctccaacga cgagcatcac cgggtgatcg aagtctgcat cgagcaggcg
     4321 gcgggccggg tgccggtgat cgccggctgc ggcagcaacg acacgatgaa cgcgctgctc
     4381 cacatggaaa cctcgcagca gctgggcgcc gatgcggcgc tggtggtggc gccgtattac
     4441 aaccggccga gccaggaggg gatgctggcg catttctcgt acctggccga gcattcggac
     4501 ctgccgatcg tgctctacaa cgtgcccggg cggacggtga ccgacctgaa gcccgccacc
     4561 gtgatcgaac tggcgcggcg gtttccgggc aagatcatcg ggatgaagga cgccagcggc
     4621 gatcccgggc gcgtgaccga gcaccggctg gggatcggcg ccgagttctg ccagatgtcg
     4681 ggcgacgacg ggctggccct gccgttcctg gccgccgggg cgactgggtg catttcggtg
     4741 acggccaatg tcgcgccggg gctctgcgcc aagtttcagg cggcgtgggc ggcgggcgac
     4801 gtggcggggg cgcgggcgct caacgaccgg ctctatccgc tccacagcgc gatgttctgc
     4861 gatgcgtcgc cggggccggt gaagtatgcc atggcccggg tgctcgactg gatggcgggg
     4921 gacctgcggc tgccgatgac cccctgcgac gaggccggtc gcaaggtggt cgacgcggcg
     4981 ctggttcatg caggattaat ctgattttat tggattaaat cagggtgggg acgggttgtt
     5041 tcgcagacgc gacaagctgc ggaacccttt gtggaaacct gtcgttaacc gtccatcatg
     5101 gccaccagac tgccctccgc cgaggaccgc cgcgtgtttg acgcacccct gcacgttgcc
     5161 cacggcgccg atggcaccgt gaccgtgttt ttccccgacg gccaccgcat ggtgttgacc
     5221 gccgaggccg ccgaacgctc gggcgcaatc ctgtggcgca ccgccctggc gctgcgcagc
     5281 cgtcggcgcg gagggccggc gcaccggtcg ggccaggtga tcgcggtcga tttcggcgaa
     5341 cacgcgcccc gccactgaac catgcgtcac taaaccagtc gccgagtctt ttgcgggcgg
     5401 ccttcccata aggagcgcgc gcccctacat gcgccggtca tggcccgccc gactcccgcc
     5461 cagttcgaca agaagaagat cgtcgcggaa aatcggcgcg cgcggttcga ctatcatatc
     5521 gacgaagtgt tcgaggcggg catcgcgctg acgggcaccg aggtgaagag cctgcgcttc
     5581 ggcgagggct cgatcgccga gtcctatgcc gaggtgaaga acggcgaggt ctggctggtc
     5641 aattccaacg tgcccgaatt cagccatggc aaccgcttca accatgagcc caagcgcctg
     5701 cgcaagctgc tgctgaaaga gcgcgagatc aacaagttcg tcggcgcgac cgagcgcaag
     5761 gggatgaccc tcgtgccgct ctcgatctac ttcaacagcc gcggccgggc gaaagtcgag
     5821 ctggcgctcg ccaagggcaa gaatgccgcc gacaagcgcc agacggtcaa ggaccgcgac
     5881 tggaagcggg agcagggccg cctgatgcgc gagcatgggt gacggcccgg atgacgacct
     5941 gaaaacttgt tcgccgcacg gattgttcct ttcatcgcag cgaaacctgt tcgcggctaa
     6001 aggacgatat cggcgcaccg cgaaaccccc tatcgtggcg caccgggacg gacagggctg
     6061 atggtcacga aattcacctc ttgggccaag cgcaagatgc caacgcgcga ggacatggag
     6121 cggagccgct tcatccgtcc cttcgcgcac cgcgtgctgg cgagcgaggt gtggcggttt
     6181 acccgccgct cggtcccgcg cggggtggcg ctgggcatgc tggtggggat catcgttccc
     6241 ttcgcccagg tgctgttcgc cgcgttcttt tcgctgagcg tacgggccaa cgtgccggtg
     6301 gcggcgctga ccaccttcat caccaacccg ttcaccaccc cgttcatctg ggcggcgagc
     6361 tacaaggtgg gtgagcggct gctctatttc gacggcatgt tccacgccaa cccgatcgac
     6421 agcctgatgc gggtgaccga cggctggcag ctgatcgaat ggatcaccgc cgagggcaag
     6481 gtgctggcac tcggcctgat cgtcgtcgcg gtgattgcgg cggcggtttc ctacctggcg
     6541 gcgagtttcg gctggaacgc ctgggtgcgg accaagcgcc gcaggcggcg gcaggcgcgg
     6601 gcggcgcagg gcgcatgacc acccggctga tcggctggct tcggtcgcac gtccccaccc
     6661 gcgaggcgct ggagcgcaac cgcttcgtgc ggcccttcgc gcatctcatc ctgcgcagcg
     6721 aactgtggcg gtttacccgc cgctcagtcc cgcgcggggt cgccctgggg ctgttcgtgg
     6781 gcgtgatgat cccgctggcg catttcgtcg tcgccgcgtt cctggccgtg ttcctgcgcg
     6841 ccaatattcc cgcggccatg ctggcgacct tcatcggctt ccccgtgatc tacgtcgccc
     6901 tcgtcgcgct ggcctacaag attggcgagt ggctgttgca ccttgacgcg atgacgggca
     6961 tccagccgct gggggaaacg atgcagacca cccagacc
//
LOCUS       DATNBV010000061         2067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1957284,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000061 DATNBV010000000
VERSION     DATNBV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957284"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..977)
                     /locus_tag="VN627_01425"
     CDS             complement(111..977)
                     /locus_tag="VN627_01425"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HXD01944.1"
                     /translation="MQKTLASAQSRLFETAAGGRLNYLEPKGDPGFYGPDSVAWRVHA
                     NPISLAIGGVAAVILELAEPRVRSGVWDHSTFRVDPLNRMKRTSEAAMITTYGSTAMA
                     QARVDMVTRMHQRVSGETPEGHTYTALEPELMTWVHVTAGYGFLNAYVRYVDPHLSVA
                     DQDRYYAEGARLGRAFGAVDPPASVADVEACFQAIRPKLRPHPIIGEFLDIVSNTSPL
                     GPAGRVLQPMVVQASIALLPQWVRDIAELPSRPLAHAAVRPVLKGLARAAGLVPGDIQ
                     KQAYARVDRRLP"
     gene            complement(987..>2067)
                     /locus_tag="VN627_01430"
     CDS             complement(987..>2067)
                     /locus_tag="VN627_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD01945.1"
                     /translation="TSVFTDKRHSLAWQKILHARGWAAPAWPKAYGGPGWSEMQRSIF
                     SAECAAAGAPNLAPMGLRMIGPCLMGYGTAEQKAYYLPRILSGEDYWCQGYSEPGAGS
                     DLAALQLRAESDGDDYVLTGSKLWTTHAHWANLMFCLVRTGFEGKPQAGITFLLIDMT
                     TPGLAVDPIVTLAGEHEVNQVFFEGVRVPKANRLGAENEGWTVAKYLLEFERGGGSAP
                     GLKVGLGRVRAIAEAEGALADPAFRRKLAEAEIAVAAIDMSERRVLSALAGGKNPGPA
                     SSMLKINGTEAMQRADELGVEAAGAYAAVFQPQAAEPGSNVEPVGPDHSLAVMARYLN
                     DRAASIYGGSNEIQRDIIARLVLGL"
BASE COUNT          316 a          703 c          717 g          331 t
ORIGIN      
        1 atgcaagagt tcctccccgt ggccgcgcct cattgggcgt cggctcatcg gtggagcgtg
       61 ctgatcttgg ctgccggcaa caagctagcg gaagtgttag gaagcgaacc ctacggcagg
      121 cggcgatcga cccgggcgta agcctgcttc tggatgtcgc ctgggacgag gccggcggcg
      181 cgcgcgaggc ccttcaggac cggtcggact gctgcgtgcg ccagaggcct ggacggtagt
      241 tccgcgatat cccgcaccca ttgcggcaag agggcgatgc tggcctgcac gaccatcggc
      301 tgcagcaccc tgcccgccgg gcccagcgga gaggtgttgg agacgatgtc gaggaactcg
      361 ccgatgatcg ggtgaggccg aagctttggc cggatggcct ggaagcaggc ctcgacatca
      421 gcgacggacg ccggcggatc gaccgcgccg aacgcccggc cgagccgcgc cccttcggcg
      481 tagtagcggt cctggtcggc cacgctgagg tgcgggtcca cgtagcggac gtaggcgttg
      541 aggaacccgt agccggcagt cacatggacc caggtcatca gctccggctc gagcgccgta
      601 taggtgtggc cctcgggcgt ctcgcccgac acccgctggt gcatgcgggt caccatgtcc
      661 acgcgcgcct gcgccatggc ggtggacccg taggtggtga tcatcgccgc ctcgctggtg
      721 cgtttcatgc ggttcagcgg atcgacgcgg aacgtcgagt ggtcccagac gccggagcgc
      781 acgcgcggct cagccagttc caggatcacc gcggccacac cgccgatagc cagcgagatc
      841 ggattggcgt gcacccgcca ggccaccgaa tccggaccgt agaaccccgg atcgcccttc
      901 ggctcaaggt agttgagccg ccccccggcc gccgtctcga agaggcggct ctgggctgag
      961 gcgagagtct tctgcatctg agtttcctag aggccgagca ccaggcgggc gatgatgtcc
     1021 cgctggattt cgttggagcc gccatagatc gaggcggccc gatcattgag atagcgcgcc
     1081 atcacggcca ggctatggtc tggacccacc ggttcgacgt tcgatcccgg ctcggcggct
     1141 tgcggctgga acaccgcggc ataggcgcca gcggcctcga cgcccaattc atcggcccgc
     1201 tgcatggcct ctgtgccgtt gatcttcagc atcgacgagg cgggcccggg gttcttgccg
     1261 ccggcgagcg cgctcagcac gcggcgctcg gacatgtcga tggccgccac ggcgatctcc
     1321 gcctcggcga gcttgcggcg gaacgcgggg tcggccagcg cgccttcggc ctcggcgatg
     1381 gctcgcacgc ggccgaggcc gaccttgagc cccggcgccg acccgccgcc ccgctcgaat
     1441 tccagcaggt acttggccac cgtccagccc tcattctcag cgccaagccg attggccttc
     1501 ggaacccgga cgccctcgaa gaacacctga ttgacctcgt gctctccggc gagcgtcacg
     1561 atcggatcga cggccagccc gggcgtggtc atgtcgatca ggaggaaggt gatcccggcc
     1621 tggggtttgc cctcgaagcc tgtgcgcacc aggcagaaca tcaggttcgc ccagtgggcg
     1681 tgcgtcgtcc aaagcttgga gccggtcagg acgtagtcgt cgccgtcgct ctccgcgcga
     1741 agctgcagcg cggcgagatc ggatcctgcg cccggctcgg aatagccctg gcaccagtag
     1801 tcctcgcccg acaggatgcg cggcaggtag tacgccttct gctcggctgt cccgtagccc
     1861 atcaggcagg gcccgatcat gcggaggccc atcggcgcaa ggttcggcgc gccggctgcg
     1921 gcgcactcgg ccgagaagat cgagcgctgc atctcgctcc agcccggccc gccgtatgcc
     1981 ttcggccagg ccggcgcggc ccagccgcgg gcgtgcagga ttttctgcca ggcgaggcta
     2041 tggcgcttgt cggtgaagac gctggtg
//
LOCUS       DATNBV010000062         3168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_356452,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000062 DATNBV010000000
VERSION     DATNBV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356452"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            520..1020
                     /locus_tag="VN627_01435"
     CDS             520..1020
                     /locus_tag="VN627_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01946.1"
                     /translation="MPHVVATCEASHLVTCLPGETRVETPVSIKAVNHIRLLIHDIAE
                     PIDGYIAPDAEHVAQLIDFAAAWDRTGPMVIHCWAGISRSTAAAFIMLCTLNPEVPEV
                     LVARSLREASPTAFPNRLMIRLGDAALGRAGRMQAAVEAIGRGEVVNEAIPFSLPADL
                     SLLHRS"
     gene            1171..2187
                     /locus_tag="VN627_01440"
     CDS             1171..2187
                     /locus_tag="VN627_01440"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01947.1"
                     /translation="MNTPVRYKTAALIPSPPPRAPARAPQPGDAVAVAVAAAILAVRA
                     GEPVVAVVPAQGAREESLPCGPFCPRSHGTLEGGVGAWTRAQIGVEVGFLQQLGAAAE
                     GADAEPAQPTVAVSYLALVGPRQCSDRTQASWRSWYNFFPWEDWRHGRPDCLADLLGR
                     LEAWAAEAGPPCATPSPGERRQRIRLAFGVAGAGWDEERVLDRYELLQEAGLIGPVAA
                     DGTTAPPLPRPVPQLLGDHARVLARAVGELRRAVKCRPVVFELMPDEFTLFELQRTVE
                     AVLGPHLHKQNFRRMVEGGGLVEPTGEHRLRTGGRPARLYRFRRDVLWERTAPGVRVK
                     AGRT"
     gene            2372..>3168
                     /locus_tag="VN627_01445"
     CDS             2372..>3168
                     /locus_tag="VN627_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXD01948.1"
                     /translation="MARISRRKFLQVSGPGVVAAKAMGMAGILASRTAPAYAQGATVH
                     WLRWNDFVPASDQLLRKEMLPEAEKALGIKINLETVNGNDLQPRITSAIQSGAGPDLI
                     MLNNNHPQLYADSLVDLSDIAEATGKAEGGYYPLVRANSNNGQKWLSMPWAIIGAMIA
                     YRKSWFEEAGANSFPETWEAYREVGKKLKAKGRPIGQTLGHTFGDSPTFTYPLLWSFG
                     GKEVEADGRTVAINTKEAVESVAFMTAFWKEAHDEGGLAWDDTNNNRA"
BASE COUNT          490 a         1081 c         1085 g          512 t
ORIGIN      
        1 gcggacagga cgagtccgac gcggacaatg ctcggcatcg ggtcgcgatg agtgggcatc
       61 tgctgcatcg gtctcccgca cgcttgtgga agaagggcaa ggccgcatcg gcacgcgtaa
      121 actgccggtg cggctcggcg ggggatcgct ctcgttgtcg gcggccccgg ccacgggcgc
      181 tgaaatggga gcacggaccg tttcgaggca tccccagttc aggcgagact tgggttggat
      241 ttgagcttga ttttgagccg gatttgagct ggatgtgcca agtacggcct gtgagaagtg
      301 agagaaattc cgagccagac gatagatcta ttccatttct gcaagaagaa aggggagatt
      361 tactttggac acgcgaattt tggggcttac gccgccgctg gggatggacg cgcatagacg
      421 gggtggccgg tttcaggcat actcgggaaa atcatgcacg atctcgtctc accgcttccc
      481 ggctcgccgg gccgtatttt tgtttgcccg ctccgcgcgg tgccgcacgt ggtggcgact
      541 tgcgaggcct ctcacctcgt cacctgcctg cccggcgaaa cgcgggtcga gaccccggtc
      601 tccatcaagg cggtgaacca tatccgcctg ctcatccacg acattgcgga gccgatcgac
      661 ggctacatcg cccccgatgc cgagcatgtc gcgcagttga tcgacttcgc ggcggcctgg
      721 gaccgcacgg ggccgatggt catccactgc tgggccggca tcagccgctc caccgcggcg
      781 gccttcatca tgctgtgcac gctgaatccc gaggtgcccg aggttcttgt cgcgcggtcc
      841 ctgcgcgagg cctcgcccac ggccttcccc aaccgcctga tgatccggct gggggatgcc
      901 gccttgggcc gcgccggccg catgcaggcg gcggtggagg ccatcggccg cggcgaggtc
      961 gtcaacgagg cgatcccctt ctccctgccg gcagacctgt ccctgctgca tcggtcctag
     1021 caccgccccg ctcgtccgga cgccccgcgg cgcgtccggc gtaagtgcgg ctcgcgtgca
     1081 ttggcagccc cctgcacaac cgcagggcag aaaattgcca tgatcggacg ttagccaacc
     1141 gatgatttcc aagagctgtc agaggcttgg atgaacaccc cagtgcgcta caagaccgcc
     1201 gcgctgatcc ctagccctcc gccgcgcgcc ccggcgcggg cgccccagcc cggtgacgcc
     1261 gtggccgtgg cggttgccgc cgccattctg gccgtgcgcg ccggcgagcc ggtggtcgcg
     1321 gtcgtgcccg cccagggcgc ccgcgaggaa tcgctgccct gcgggccgtt ctgccctcgc
     1381 tcgcacggta ccctggaggg cggcgtgggg gcctggacgc gtgcccagat cggcgtggag
     1441 gtcggcttcc tgcagcagct cggcgccgcg gccgagggtg ccgacgccga gccggcccag
     1501 cctaccgttg cggtgagcta cctggcgctg gtggggcctc gccagtgcag cgaccgcacc
     1561 caggccagct ggcgcagctg gtacaacttt tttccctggg aggactggcg gcacggccgg
     1621 ccggactgcc tcgccgacct tctgggccgc ctggaggcgt gggccgcgga ggctggcccg
     1681 ccgtgcgcca cgccgtcgcc gggggagcgc cgccagcgca tccggctggc attcggggtc
     1741 gcgggcgccg gctgggacga ggagagggtg ctcgaccgct acgagctgct gcaggaggcc
     1801 ggcctcatcg gaccggttgc cgcggacggc accacggcac cgcccctgcc gcggccggtg
     1861 ccccagctgc tgggcgacca tgcccgggtg ctggcgcgcg ctgtcggcga gctgcggcgc
     1921 gcggtgaagt gccgccccgt cgtgttcgag ctgatgccgg acgagttcac cctgttcgag
     1981 ctgcagcgga cggtcgaggc ggtgctcggc ccgcatctcc acaagcagaa cttccgccgc
     2041 atggtcgagg gcggcggcct cgtcgagccc accggcgagc accgtctgcg caccggcggc
     2101 cgccccgccc gcctctaccg cttccgccgc gatgtcctct gggagcgcac cgcgccaggg
     2161 gtgcgggtga aggccgggcg gacttgagcg gcgaggtgcc gtccggctcg gacatttccg
     2221 ttcactgacc cgaggctgct cccgaccggc gtcgatctca tgcaagcggc gccgcccgcg
     2281 cctgtcagcc tgcgccttgc gtcgatcggt cgtcgagcct acagttgaca gcaagctcgc
     2341 ttcgaacgcg ggcaatctgg gaggatgcaa tatggctcgc atttcgcgcc gaaaattcct
     2401 gcaagtctcg ggccccggag tggtggctgc gaaggccatg ggcatggccg gcatcctcgc
     2461 gagccgcacc gcgcccgcct atgcgcaggg cgccaccgtt cactggctca gatggaacga
     2521 ctttgtcccg gcatccgacc agctgctgcg caaggagatg ctgcccgagg ccgagaaggc
     2581 gctcgggatc aagatcaatc tggagacggt gaacggcaac gacctgcagc cgcgtatcac
     2641 ctcggcgatc cagtcgggcg cgggtcccga cctgatcatg ctcaacaaca accacccgca
     2701 gctctatgcc gatagcctgg tggacctgtc ggacatcgcc gaggcaacgg gcaaggccga
     2761 gggtggctac taccccctgg tgcgcgccaa ttccaacaac gggcagaaat ggctgtcgat
     2821 gccatgggcc atcatcggtg ccatgatcgc ctaccgcaaa tcctggttcg aggaggcggg
     2881 cgccaactcc ttccccgaga catgggaggc gtatcgggag gtcggcaaga agctcaaggc
     2941 caagggccgg cccatcgggc agacgctggg ccataccttc ggcgactcgc cgacgttcac
     3001 ctatcccctc ctgtggtcgt ttggcggcaa ggaggtggaa gccgatggcc gcaccgtcgc
     3061 catcaacacc aaggaagccg tcgagtccgt cgccttcatg acggccttct ggaaggaagc
     3121 ccatgacgag ggcggtctcg cctgggacga taccaacaac aaccgggc
//
LOCUS       DATNBV010000063         5543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1316986,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000063 DATNBV010000000
VERSION     DATNBV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316986"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VN627_01450"
     CDS             complement(<1..395)
                     /locus_tag="VN627_01450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HXD01949.1"
                     /translation="MTETFMNWGLSYEWAWGLATVAGTLLIALPLMLGVAMMIYADRK
                     IWAAMALRRGPNVVGPWGLLQSFADGLKVFLQETIIPSASNKGLFLLAPIITFTVALM
                     AWAVIPFNSGAVLADINVGLLYVLAISSLG"
     gene            complement(553..2574)
                     /gene="nuoG"
                     /locus_tag="VN627_01455"
     CDS             complement(553..2574)
                     /gene="nuoG"
                     /locus_tag="VN627_01455"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HXD01950.1"
                     /translation="MPKVTVDGTELEVPAGATVLQACELAGKEIPRFCYHERLSIAGN
                     CRMCLVEVAPGPPKPQASCALPAAEGQVIKTDSPMVKKAREGVMEFLLINHPLDCPIC
                     DQGGECDLQDQSVAYGRGASRYHEHKRAVTEKYMGPLIKTVMTRCIHCTRCVRFSEEV
                     AGVDEIGALYRGEAMQITTYLEHAARHEMSANVIDLCPVGALTSRPYAFEARPWELKK
                     TLSIDVSDAVGANIRLDSRGREVLRILPRVNDDVNEEWISDKARFEVDGLTRRRLDRP
                     WLRKNGKLVAATWDEAFAAVAKVKPGASVAAIAGDLVDCETMFAAKALVSALGSDLLE
                     GRQTGMSYAADNLAAVNFNTTLAGIENADAILIVGSHVRWEAPLVNSRLRKAVKRGAR
                     VFVVGPEYDLTFKAEFLGDDAGLLGKLPDHVTKAMTDAARPAVILGGAGLAKGALEPA
                     LALAEQWNLVRDLEDGSAWNGFNVLHMAASRMGGLMLGYAQKGGIADLVGAAPKLVLS
                     LGADEVDYAQFANSMIVYIGHHGDKGAHAADIILPGAAFTEKAGTYVNTEGRVQFAEK
                     AVFAPGDAREDWTILRALADALKVSVGFDSFAELRAAMVAAVPALGHEGLAGYGKLPK
                     APKGAKAEGAIVYPIKDHYLVNPVCRSSPTLQRCSAELLHGEEIAEAAE"
     gene            complement(2574..3875)
                     /gene="nuoF"
                     /locus_tag="VN627_01460"
     CDS             complement(2574..3875)
                     /gene="nuoF"
                     /locus_tag="VN627_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXD01951.1"
                     /translation="MALQDKDRIFTNVYGYQPWNLKAAQARGDWDNTKALLARGRDAI
                     VQEIKDSSLRGRGGAGFPTGMKWSFMPKEYKPDRPGFLVINADESEPGSCKDREIIRH
                     DPHKLIEGALVAGFAMGARAAYIYIRGEYIREAETLFAAVAEAYDAGLIGKNASGSGY
                     DFDVFVHRGAGAYICGEETAMIESIEGKKGQPRLKPPFPAGAGLYGCPTTVNNVESIA
                     VAPTILRRGAGWFSSFGAENNRGTKLFQISGHVNKPCVVEEAMSIPFEELIEKHCGGI
                     RGGWDNLLAVIPGGSSVPLVPGEQIRHVAMDFDGLKAVGSGLGTAAVIVMDKSTDIVR
                     AISRLSYFYKHESCGQCTPCREGTGWMWRVMERLRTGDAAVEEIDMLQEVTKQVEGHT
                     ICALGDAAAWPIQGLIRHFRPELERRIEANVRPNNFAEAAE"
     gene            complement(3877..4545)
                     /gene="nuoE"
                     /locus_tag="VN627_01465"
     CDS             complement(3877..4545)
                     /gene="nuoE"
                     /locus_tag="VN627_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HXD01952.1"
                     /translation="MADRHLEADTPELRARWGGWTFSEANVAKAKEIVARYPEGRQRS
                     AVMGLLDLAQRQVGAETNTQGWLPIPVMEYVADFLDMPIIRVVEVATFYTMYNLVPVG
                     RFHVQVCGTTPCMLRGSDDLISACAKRGMPTGGVSADGLWTFTEVECMGNCASAPMVQ
                     INDDNYEDLTAERLDAVLDALARGETPKTGTQEPGRHTVEPVGGPTSLTAMVSENHDY
                     RGEW"
     gene            complement(4538..4738)
                     /locus_tag="VN627_01470"
     CDS             complement(4538..4738)
                     /locus_tag="VN627_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01953.1"
                     /translation="MKTTFDLMRVWAAITGLILAAWYFGCLYNGMKPYPMLPMLIAAI
                     GGFEMVLYAQDIWFKRRGRQNG"
     gene            complement(4735..5085)
                     /locus_tag="VN627_01475"
     CDS             complement(4735..5085)
                     /locus_tag="VN627_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD01954.1"
                     /translation="MSNLKTAEAMIAAYNAQDVDTYVSYMTDEASEANYRGSVVREGK
                     EGTRSGLAAAFAKWPQNRAEVRHAQQVGDYVLFREYVTRGPATDGSELVEPFEVIAVY
                     SFEGDKCSRVEFIR"
     gene            complement(5082..>5543)
                     /locus_tag="VN627_01480"
     CDS             complement(5082..>5543)
                     /locus_tag="VN627_01480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211732.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HXD01955.1"
                     /translation="YDRMEFDIPVGTNSDCYDRFMVRVEEVRQSARIMKQCLAEMPIG
                     PVASTDRKVSPPKRAEMKASMEALIHHFKLYTEGFHVPAGEVYVATESPKGEFGVYLV
                     SDGSNKPYRCKIRPTAFSHLQAMDFMSKGHMLPDATAILGAIDVVFGECDR"
BASE COUNT          892 a         1859 c         1827 g          965 t
ORIGIN      
        1 cccagcgacg agatcgcgag gacatagagc aggccgacgt tgatatcggc cagcacggca
       61 cccgagttga acgggatcac cgcccaggcc atcagcgcga cggtgaaggt gatgatcggc
      121 gcgagcagga acaggccctt gttcgaagcg ctcgggatga tggtttcctg gaggaacact
      181 ttcaggccat ccgcgaacga ctgcagcagg ccccacgggc ccaccacgtt cggcccgcgc
      241 cgcagcgcca tcgccgccca gatcttgcgg tcggcataga tcatcatcgc cacgccgagc
      301 atcagcggca gcgcgatcag cagggtgccc gcgactgtcg ccaggcccca ggcccattca
      361 taggacaggc cccagttcat gaaggtctcg gtcacgctca gatcctcccc actcgtgggg
      421 aggtggcagc ggcgcagccg ctgacggagg gggccatcgg agcggcgcca cgctgcgtca
      481 ggaggcaaac cccctccacc accctgcggg tggtccccct ccccgtcccg gggaggattt
      541 cagatgcccc gatcattccg ccgcctccgc gatttcctcg ccgtggagca gttcggccga
      601 gcagcgctgc agcgtcgggc tcgaacggca caccgggttg acgaggtagt ggtccttgat
      661 cgggtacacg atcgcaccct cggccttggc ccctttcgga gccttcggca gcttgccgta
      721 gccagccaga ccctcatggc ccagcgccgg aaccgcggct accatcgcgg cgcgcagttc
      781 cgcgaagctg tcgaagccga ccgaaacctt cagcgcatcg gccagcgcgc gcaggatggt
      841 ccagtcctcg cgcgcgtctc cgggcgcgaa cacggccttc tcggcaaact gcacgcgccc
      901 ttcggtgttg acgtaggtcc cggccttctc ggtgaaggcc gcaccgggca ggatgatgtc
      961 cgccgcatgg gcgcccttgt cgccatggtg gccgatgtag acgatcatcg aattggcgaa
     1021 ctgggcatag tccacctcgt ccgcgccaag cgagagcacc agcttgggcg ccgcgccgac
     1081 gaggtcggcg atgccgccct tctgggcata gcccagcatc agcccgccca tgcgactggc
     1141 cgccatgtgc agcacgttga agccgttcca ggccgaaccg tcctcgaggt cacgcaccag
     1201 gttccactgc tcggccagcg ccagcgccgg ctcgagcgcg cccttggcca gccccgcgcc
     1261 ccccaggatc accgcaggcc gcgccgcgtc ggtcatggcc ttggtgacat gatccggcag
     1321 cttgcccagc aggccggcat cgtcgccgag gaactcggcc ttgaaggtca ggtcgtattc
     1381 cgggccgacc acgaagacgc gggcgccgcg cttgaccgcc ttgcgcaggc ggctgttcac
     1441 cagcggcgct tcccagcgga catggctgcc gacgatgagg atcgcgtcgg cgttctcgat
     1501 gccggcgagc gtggtgttga agttcaccgc ggcgaggttg tcggcggcat agctcatgcc
     1561 ggtctggcgg ccttcgagca ggtcggaccc gagcgcggag accagcgcct tggcggcgaa
     1621 catggtctcg cagtcgacga gatcaccggc gatcgcggcg acgctggcgc cgggcttgac
     1681 cttggcgacg gcggcgaaag cttcatccca ggtggcggcg acgagcttgc cgttcttgcg
     1741 cagccacggg cggtcgaggc ggcgcctggt caggccatcg acctcgaagc gggccttgtc
     1801 ggagatccac tcctcgttca cgtcgtcgtt gacgcggggc aggatgcgca ggacctcgcg
     1861 gccgcggctg tcgagccgga tattggcgcc gaccgcgtcg gagacgtcga tcgacagggt
     1921 tttcttgagc tcccacggcc gcgcctcgaa ggcatagggg cgcgaggtga gcgcgccgac
     1981 cgggcagagg tcgatcacgt tggccgacat ctcgtgcctg gcggcatgtt cgagataggt
     2041 cgtgatctgc atcgcctcgc cgcgatagag cgcgccgatc tcgtccaccc cggcgacttc
     2101 ttcggagaag cgcacgcagc gggtgcagtg gatgcagcgg gtcatcaccg tcttgatgag
     2161 gggccccatg tacttctcgg tcaccgcgcg cttgtgctcg tgatagcgcg aggccccgcg
     2221 gccataggcg accgactggt cctgcaggtc gcactcgccg ccctgatcgc agatcgggca
     2281 gtcgagcggg tggttgatga ggagaaactc catcacccct tcccgcgcct tcttgaccat
     2341 cgggctgtcg gtcttgatga cctgtccctc ggcggcgggc agcgcgcagc tcgcctgcgg
     2401 cttcggcggc ccgggggcga cctcgaccag gcacatgcgg cagttgccgg cgatgctgag
     2461 gcgctcgtga tagcagaagc gcgggatttc cttgccggcg agttcgcaag cctgcagaac
     2521 ggtggcgcct gccgggactt cgagttcggt gccgtcgacg gttaccttgg gcattactct
     2581 gcggcctccg cgaagttatt tggccgcacg ttcgcttcaa tccgccgctc cagctcgggg
     2641 cggaagtggc ggatcaggcc ctggatcggc caggcggccg cgtcgcccag ggcgcagatg
     2701 gtgtggcctt cgacctgctt ggtgacttcc tggagcatgt cgatctcctc gaccgccgcg
     2761 tcaccggtgc gcagacgctc catgactcgc cacatccagc cggtgccttc gcggcacggc
     2821 gtgcactggc cgcaggactc gtgcttgtag aagtaggaca ggcgggagat cgcgcggacg
     2881 atgtcggtcg acttgtccat gacgatgacc gccgccgtgc cgaggcccga gccgacggcc
     2941 ttcagcccgt cgaaatccat cgccacgtgg cggatctgct cacccgggac cagcggcacc
     3001 gaggaaccgc cggggatcac cgccagcagg ttatcccacc cgccgcgaat gccgccgcag
     3061 tgcttttcga tcagttcctc gaacgggatc gacatcgcct cttcgacgac gcagggcttg
     3121 ttcacgtggc cgctgatctg gaacagcttg gtcccgcggt tgttctccgc gccgaagctg
     3181 gagaaccagc ccgcgccgcg gcgcaggata gtcggcgcga cggcgatcga ttccacgttg
     3241 ttcaccgtgg tcgggcagcc atagaggccg gcgccggccg ggaacggggg cttgaggcgc
     3301 ggctggccct tcttgccctc gatgctctcg atcatcgcgg tctcttcgcc gcagatgtag
     3361 gcacccgcgc cgcggtggac gaagacgtcg aaatcgtagc ccgaaccgct cgcattcttg
     3421 ccgatcagcc cggcgtcata ggcctcggcc acggccgcga acagcgtctc ggcctcgcgg
     3481 atatattcgc cgcggatgta gatgtaggcc gcccgcgcgc ccatcgcgaa gccggccacc
     3541 agcgcgccct cgatcagctt gtgcggatcg tggcggatga tctcgcggtc cttgcacgaa
     3601 ccgggttcgg actcgtcggc gttgatgacg aggaagcccg gacggtccgg cttgtattcc
     3661 ttgggcatga aggaccactt catgccggtc gggaagcccg cgccgccgcg cccgcgcagg
     3721 ctggaatcct tgatttcctg aacgatcgca tcgcgcccgc gcgccagcag ggccttggtg
     3781 ttgtcccaat cgccgcgtgc ttgcgctgcc ttcaggttcc acggctggta gccatagacg
     3841 ttggtgaaga tgcggtcctt gtcctggaga gccatcctac cactcccccc ggtaatcatg
     3901 gttttcgctg accatggcgg tgagcgaggt cggcccgccg acgggttcga ccgtgtggcg
     3961 gccgggctcc tgggtgccgg tcttcggcgt ctcgccgcgg gcgagcgcat cgaggacggc
     4021 gtcgagccgc tcggcggtca ggtcttcata gttgtcgtcg ttgatctgga ccatcggggc
     4081 cgaggcgcag ttgcccatgc attcgacctc ggtgaaggtc cacaggccat cggccgagac
     4141 cccgccggtc ggcatgccgc gcttggcgca ggccgaaatc agatcgtccg agccgcgcag
     4201 catgcacggc gtggtgccgc agacctgcac gtggaagcgg cccaccggca cgaggttgta
     4261 catggtatag aaggtcgcca cctcgaccac gcggatgatc ggcatgtcga ggaaatccgc
     4321 gacatattcc atcaccggga tcggcagcca gccctgggta ttcgtctccg cgccgacctg
     4381 gcgctgagcc aagtcgagca ggcccatcac cgccgagcgc tggcgccctt cgggatagcg
     4441 cgcaacgatt tccttcgcct tggcaacgtt cgcttccgag aaggtccacc ctccccagcg
     4501 cgcccgcagc tcgggcgtat ccgcctcgag atgcctgtca gccattttgg cgcccccggc
     4561 gcttgaacca gatgtcctgc gcgtagagga ccatctcgaa gccgccaatc gccgcgatca
     4621 gcatgggcag catcggatag ggcttcatgc cattgtaaag gcagccgaaa taccaggcag
     4681 ccaggatcag gcccgtgatt gccgcccaca cacgcatcag gtcgaaagtg gtcttcaccg
     4741 gataaactcc acgcgagagc acttgtcgcc ctcgaaagaa tagactgcga tgacttcaaa
     4801 gggctcgacc agttccgaac catcggtagc gggcccccgc gtgacgtatt cgcggaacag
     4861 gacgtaatcg ccgacctgct gcgcatgacg cacttccgca cggttctgcg gccacttggc
     4921 gaaagcggcg gcaaggcccg aacgggtccc ttccttgcct tcgcgcacga cggagccgcg
     4981 atagttcgcc tcgctggctt cgtcggtcat gtaggagacg taggtatcga cgtcctgcgc
     5041 attgtaggcg gcgatcatcg cctctgcggt cttgagatta ctcaccggtc acactccccg
     5101 aacaccacgt cgatcgcgcc gagaatggcg gtcgcatcgg gcagcatgtg gcccttgctc
     5161 atgaagtcca tcgcctggag gtggctgaag gcggtcggcc ggatcttgca gcggtagggc
     5221 ttgttgctcc cgtcgctcac cagatagacg ccgaattcgc ccttgggact ttcggtggcg
     5281 acgtaaactt cgcccgccgg cacgtggaag ccttcggtgt agagcttgaa gtggtggatg
     5341 agggcttcca tcgaggcctt catctccgcg cgcttgggcg ggctgacctt gcggtcggtg
     5401 ctagcgaccg ggccgatggg catttcggcg aggcactgct tcatgatccg ggcggactgg
     5461 cgcacttcct cgacgcggac catgaaccgg tcgtagcaat cggaattggt gcccaccggg
     5521 atgtcgaact ccatccggtc gta
//
LOCUS       DATNBV010000064         3654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3549647,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000064 DATNBV010000000
VERSION     DATNBV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3549647"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /gene="ppk1"
                     /locus_tag="VN627_01485"
     CDS             <1..1582
                     /gene="ppk1"
                     /locus_tag="VN627_01485"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HXD01956.1"
                     /translation="PALPRYVRIPGEAAQWIGIADLIARHAGALFPGIRVLGSGTFRV
                     LRDSDIEIEEDAEDLVRYYRTAIQRRRRGKVIMLQLQQDFDPAAEALLREQLDLGNAL
                     VLPMGRQVDFTGLAAIIDEPRPDLKFPPYSPRYPERIREHDGDCFAAIREKDIVIHHP
                     YESFEVVIDFLRQAAQDPDVVAIKQTLYRAGKQSAVIAALIAAAEAGKSVTAVVELKA
                     RFDEEQNLLWASQLERAGVQVIYGFVDWKTHAKVSVVVRREGESYRTYCHFGTGNYHP
                     VTARIYTDLSFFTADPKAGRDAGALFNFITGYVEPKSTELLAISPINLRAKLYECIDA
                     EIAFARAGKPATIWAKLNQLTDEALIERLYDASRAGVEVRLVVRGICCLRPGVPGVSE
                     RITVKSIIGRFLEHSRIWAFGNGADLPNAKARVYISSADGMSRNLDRRVEVLVPIRNR
                     TVHDQVLDQVMLANLLDTEQSWILAPDGSYRRLEAGPKPFNLHKYFMTNPSLSGRGAA
                     LASGRKVPKLALRRGGGR"
     gene            1614..3089
                     /locus_tag="VN627_01490"
     CDS             1614..3089
                     /locus_tag="VN627_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HXD01957.1"
                     /translation="MAGVSETEANDLAPARAIIDVGSNTVRLVIYGGPDRAPVVLHNE
                     KVTARLGKGVAETGRLSKKAAASALAALARYRCLLDLKGVTRVDLVATAAVRDAADGP
                     EFLARIAALGYAPRLLAGEEEALTSAAGVLGAFPGAHGVVADLGGGSLELVDIADDRA
                     EHGVSLPLGTLRLPPLRAGTHQQFLDRIDAVIAQARWTTPPGATLYLVGGSFRAFARA
                     AMQQLAWPGDDPHGFALPADKALAMARGLAGQPAETLAPLPGISASRLAVLPDASAML
                     HALIRRLAPARLVFSSWGLREGVLIASLDAATRAEDPLAAGIAAFAAHQGCTAEEAAL
                     VARWTAPVLPATDESLRLPATALALASAMVEPNLRTEIAVTWALRKRWIGIDARGRAM
                     LAAALMANTARLDLPKPWRQLAPAADLAAAQAWGLAVRLCRRFSGGSSAALAGSAVAI
                     DGKALRLTVRPAYAALVNEGVERDLKSLAQHLGLKPATEIT"
     gene            complement(3116..>3654)
                     /locus_tag="VN627_01495"
     CDS             complement(3116..>3654)
                     /locus_tag="VN627_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HXD01958.1"
                     /translation="LVGGKYWAQTRPLGDLYFAYPPGSQWRSGPSINLATSPDGRHWQ
                     PHLMPGIRPHAGTVATARMGGGTPPILTKVAGRRGWLTLWHGVEPKEIVGIYRTYWSL
                     LDEHEPWRVIAGSHAALLEASPALTEPLEELMYLRDVVFTTGIVDAGGHFIVASGEAD
                     LACRITHVPKGRLSGEAI"
BASE COUNT          537 a         1464 c         1118 g          535 t
ORIGIN      
        1 cccggccctg ccgcgctacg tgcgcattcc gggcgaggcg gcgcagtgga tcggcatcgc
       61 cgatctcatc gcccgccatg ccggcgctct gttccccggc atccgcgtgc tcggttcggg
      121 caccttccgg gtcctgcgcg attcggacat cgagatcgag gaggatgccg aggatctcgt
      181 gcgctactac cgcaccgcga tccagcgccg ccgccggggc aaggtgatca tgctccagct
      241 ccagcaggac ttcgatcccg ccgccgaggc cctgctgcgc gaacagctcg atctcggcaa
      301 tgcgctggtc ctgccgatgg gccgccaggt cgatttcacc ggcctcgccg cgatcatcga
      361 cgagcctcgc cccgatctca agttcccgcc ctattccccc cgctaccccg aacgcatccg
      421 cgagcacgat ggcgattgct tcgcggcaat ccgcgagaag gacatcgtca tccaccaccc
      481 ctacgagtcc ttcgaggtgg tgatcgattt cctccgccag gccgcgcagg accctgacgt
      541 ggtcgcgatc aagcagacgc tctaccgcgc cggcaagcag tccgccgtca tcgccgccct
      601 catcgccgcg gcggaagccg gcaagtcggt gaccgccgtg gtcgagctca aagcccgctt
      661 cgacgaggag cagaacctcc tctgggccag ccagctcgaa cgcgccgggg tccaggtgat
      721 ctacggcttc gtcgactgga agacccatgc caaggtctcc gtggtggtgc gccgcgaggg
      781 cgagagctac cgcacctact gccacttcgg caccggcaac taccatccgg tcaccgcccg
      841 catctatacc gacctgtcgt tcttcaccgc cgatcccaag gccggccgcg atgccggcgc
      901 gctgttcaat ttcatcaccg gctatgtcga acccaagagc acggaactgc tcgcgatctc
      961 cccgatcaac ctgcgcgcca agctctacga atgcatcgac gccgagatcg ccttcgcccg
     1021 cgccggcaag cccgccacga tctgggccaa gctcaaccag ttgaccgacg aggccctgat
     1081 cgagcggctc tacgacgcca gccgcgccgg agtcgaggtc cgcctcgtcg tgcgcgggat
     1141 ctgctgcctg cgccccggcg tccccggcgt gtccgagcgg atcaccgtca agtcgatcat
     1201 cggccggttc ctcgaacaca gccgcatctg ggccttcggc aacggggccg acctgcccaa
     1261 tgccaaggcc cgggtctaca tctcctcggc cgacgggatg agccgcaacc tcgatcggcg
     1321 cgtcgaagtc ctcgtcccca tccgcaaccg caccgtgcac gatcaggtgc tcgaccaggt
     1381 catgctcgcc aacctgctcg ataccgagca atcctggatc ctcgcgcccg acggctccta
     1441 ccgccgcctc gaagccggcc cgaaaccgtt caacctccac aagtatttca tgaccaaccc
     1501 ctcgctttcc gggcgcgggg ctgctttggc gagcgggcgc aaggtgccca agctcgccct
     1561 gcgccgcggc ggcgggcgtt aagaccttcc gcaaaccggg tggaacgaag ggcatggccg
     1621 gcgtgagcga gactgaagcc aacgatctgg cgcccgcgcg cgcgatcatc gatgtcggct
     1681 ccaacaccgt gcgcctcgtg atctacggcg ggccggaccg cgcgcccgtc gtgctccaca
     1741 atgaaaaggt caccgcccgc ctaggcaagg gcgtcgccga aaccgggcgc ctgtcgaaga
     1801 aggccgccgc ctcggccctg gccgcgctcg cccgctatcg ctgcctgctc gacctgaagg
     1861 gcgtgacccg ggtcgatctc gtcgccacgg ccgccgtgcg cgatgcggcc gacgggcccg
     1921 agttcctcgc ccgcatcgcc gccctcggct atgccccgcg cctgctggcc ggggaagagg
     1981 aagccctcac cagcgccgcc ggcgtgctcg gcgcctttcc cggggcccac ggcgtggtcg
     2041 ccgatctcgg cggcggcagc ctcgaactgg tcgatattgc cgatgaccgg gccgaacacg
     2101 gcgtcagcct ccccctcggc accctgcgcc tgccgccgct gcgcgccggc acccaccagc
     2161 agttcctcga ccggatcgat gcggtcatcg cgcaggcccg gtggaccacg ccgcccggcg
     2221 ccacgctcta cctcgtcggc ggctccttcc gggccttcgc ccgcgccgcc atgcagcagc
     2281 tcgcctggcc gggcgacgat ccccacggct tcgccctccc cgccgacaag gccctcgcca
     2341 tggcccgcgg cctcgccggc caacccgccg aaacgctcgc tcccctcccc ggcatcagcg
     2401 cctcgcgcct cgccgtcctg cccgatgcct cggccatgct tcacgcgctg atccgccgcc
     2461 tcgcgcccgc ccggctggtc ttttcctcct ggggcctgcg cgaaggcgtg ctcattgcca
     2521 gcctcgatgc ggcaacccgc gccgaggacc cgctcgccgc cggcatcgcc gccttcgccg
     2581 cccaccaggg ctgcactgcc gaggaagccg cgctggtggc ccgctggacc gcaccggtcc
     2641 tgcccgccac ggacgagagc ctgcgcctcc cggccacggc cctggccctc gcctcagcca
     2701 tggtcgagcc caacctgcgc accgagatcg cggtgacctg ggccctgcgc aagcgctgga
     2761 tcggcatcga cgcccgcggc cgcgccatgc tcgccgccgc gttgatggcc aacaccgccc
     2821 ggctcgacct gcccaagccc tggcgccagc tcgcccccgc cgccgatctc gccgccgcgc
     2881 aagcctgggg cctcgcggtg cgcctgtgcc gccgcttctc cggcggcagc agcgcggcac
     2941 tggccggctc ggccgtggcg atagacggca aggcgctacg tctgaccgtc cgcccggcct
     3001 atgccgcact ggtcaacgaa ggcgtcgagc gcgacctcaa gtccctcgcc caacacctcg
     3061 gcctcaagcc ggcaaccgag atcacctgaa ttccttcccc cgcgatgggg gcaggctaga
     3121 tcgcctcacc cgacaagcgc cccttgggca catgggtaat ccggcaggcc aggtccgcct
     3181 cccccgatgc cacgatgaaa tgcccgcccg catccacgat cccggtcgtg aacaccacgt
     3241 cgcgcagata catcagctcc tccagcggct cggtcagcgc cgggctggcc tccagcagcg
     3301 ccgcatggct cccggcaatc acccgccaag gctcgtgctc gtcgaggagg ctccagtagg
     3361 tccggtagat gccgacgatc tccttcggct caaccccgtg ccacagcgtc agccagccgc
     3421 gccggcctgc caccttggtc aggatcggcg gtgtgccgcc ccccatccgc gccgtggcga
     3481 ccgtgccggc gtggggccgg atgccgggca tcaggtgtgg ctgccagtgc cgcccgtccg
     3541 ggctggtggc gaggttgatc gacggccccg agcgccactg cgagcccggc ggataggcaa
     3601 aatagagatc gcccaggggc cgcgtctgcg cccagtactt gccgcccacc agcc
//
LOCUS       DATNBV010000065         3393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_356746,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000065 DATNBV010000000
VERSION     DATNBV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356746"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VN627_01500"
     CDS             <1..1311
                     /locus_tag="VN627_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein"
                     /protein_id="HXD01959.1"
                     /translation="DLAEQVTAAADEVTEAEHKRALLPDPATGRAALAAAQDRHNAAR
                     LSLQSAHAALAGHDQQQAAARERVAAQRAEVRGWQARAGDAATRLAEMDRRVTEIAEE
                     RAVVAAKPPALEAEIEAAEETRLRLAAELAQADGQVQQATAAQAEADKVLAAANEALS
                     QARETRAGAAARAENEDQRRLEMTRISGERFQCPPPLLPERFAFAAEDVAGAAEEAAA
                     MDRLTAERERIGPVNLVAAEELKEAEGRHGESVAEQAELTEAVNRLRGSIGNLNREGR
                     ERLRAAFEAVDGHFRHLFTRLFQGGQAHLALVDSDDPLEAGLEIFAQPPGKRLQSLTL
                     LSGGEQALTAVALIFALFLTNPAPICVLDEVDAPLDDANIERFCDLLEAMVQETDTRY
                     LIVTHNAVTMSRMHRLFGVTMVEKGVSRLVSVDLGGAEQLLAAE"
     gene            complement(1564..>3393)
                     /locus_tag="VN627_01505"
     CDS             complement(1564..>3393)
                     /locus_tag="VN627_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXD01960.1"
                     /translation="MVDADGRIMAATSGFALRATGDPAAALVGMEFVSLLRQDDHDRI
                     SWERDGARGGPLTMYYVPVIDPDAAHAPDPDTAAALMLLVDSGMGIGAGASSAEGHAA
                     VPHLEALLAQLPLGLAMADRDGRLLFANAAFMRAAGRENELPPPFPSDLVVREDKAAL
                     SDAVRRHAQGPAAAGDIAVRLKGQPEDPVSLSLATVRGLGEAAVLLGLTDTTEETRLK
                     RQVAQATKMQAVGQLAGGVAHDFNNVLTAILGTCDLMLLRHTPGDSDYDDIQQIRANS
                     NRAASLTRQLLAFSRQQTLRPQVLQLPDVVAEVSQMLKRLIGEKIRFEVRHDRDLGPV
                     RADPTQLEQVIVNLVVNARDALMGKVESGAKPDGARLTITTRRVSSADVRAMRSEILP
                     QGDYTGLIIEDTGSGIAPDHLAKIFEPFFTTKEQGRGTGLGLSTVYGIVKQSGGFIFA
                     DSEVGKGTRFSIYFPVHHGPAQAPVVAKPEEPSKWSGGGRILLVEDEESVRAVAERSL
                     TRQGYEVTSAGDGEEGLEYVRLGRRFDLVVSDVVMPVMDGPAMAREIRAVAPDMPVLF
                     MSGYAEEQLRKEIGLANMHFLPKPLSVQQICDKVAEVMGAVRG"
BASE COUNT          529 a         1302 c         1076 g          486 t
ORIGIN      
        1 gacctcgccg agcaggtcac cgccgccgcc gacgaggtca ccgaggccga gcacaagcgc
       61 gccctcctgc ccgatcccgc caccggccgc gctgccctgg ccgccgcgca ggatcgccac
      121 aacgccgccc gcctctccct ccagtccgcc catgcggcgc tcgccggcca cgaccagcag
      181 caggccgccg cgcgcgaacg cgtcgccgcc cagcgcgccg aagtccgcgg ctggcaggcc
      241 cgcgccggcg atgccgccac ccgccttgcc gagatggacc gccgcgtcac cgaaatcgcg
      301 gaggaacgcg ccgtcgtcgc cgccaagccc cccgcccttg aggcagagat cgaggccgcc
      361 gaggaaaccc gcctccgcct cgccgccgaa ctcgcgcagg ctgacgggca ggtccagcag
      421 gccaccgccg cgcaagccga ggccgacaag gtcctcgccg cggctaacga agctctcagc
      481 caggctcgcg aaacccgtgc cggtgccgcc gcccgcgccg aaaacgagga tcagcgccgc
      541 cttgagatga cccgcatctc cggcgaacgc ttccagtgcc cgcccccgct cctccccgaa
      601 cgcttcgcct tcgccgccga ggacgtcgcc ggcgccgccg aggaagccgc cgcgatggac
      661 cgcctcacgg ccgagcgcga gcggatcggc ccggtcaacc tcgtcgcggc cgaggaactc
      721 aaggaagccg aagggcgcca cggcgaaagc gtggccgaac aggccgaact caccgaagcg
      781 gtcaatcgcc tgcgcggttc gatcggcaat ctcaaccgcg aaggccgcga acgcctgcgc
      841 gccgccttcg aggcggttga cggccacttc cgccacctgt tcacccgcct gttccagggg
      901 ggacaggctc acctcgccct cgtcgattcc gacgatccgc tcgaagccgg cctcgaaatc
      961 ttcgcccaac cgccgggcaa gcgcctgcaa tcgctcacgc tcctctccgg cggcgaacag
     1021 gcgctcaccg ccgtcgccct gatcttcgcc ctgttcctca ccaaccccgc cccgatctgc
     1081 gtcctcgacg aagtcgacgc gcccttggac gacgccaata tcgaacgctt ctgcgacctg
     1141 ctggaggcga tggtccagga gacggacacg cgttacctga tcgtcaccca caacgcggtg
     1201 acgatgagcc ggatgcaccg gctgttcggg gtaaccatgg tggaaaaggg cgtctcgcgg
     1261 ctggtcagcg tggaccttgg cggggcggag cagctgctcg cggcggagta gctactcaat
     1321 cactcccctc ccgcaggcgg aagggtgctt ttctctcact cccctcccgc aggcggaagg
     1381 gcgcttttct ctcactcccc ttccgcaggc gggagggcac ttttctctca ctcccctccc
     1441 gcaggcggga ggggttgggg gtgggcgcgc gcagcgctgg gccgcagacc caccccgctg
     1501 cgactagggc gcttcgcacc caagtctcgc tgcccctccc gcctgcggga ggggagaaat
     1561 gccctacccc ctaaccgccc ccatgacctc cgccaccttg tcgcagatct gctgcaccga
     1621 taacggcttg ggcaggaagt gcatgttggc gaggccgatc tccttgcgca gctgctcctc
     1681 ggcatagccc gacatgaaca gcaccggcat gtccggcgcc accgcccgga tctcgcgggc
     1741 catggcgggc ccgtccatca ccggcatcac cacgtcggaa accaccaggt cgaaccgccg
     1801 cccgagccgg acatattcga gcccttcctc gccgtccccg gccgaggtca cctcatagcc
     1861 ctgccgggtg agcgacctct cggccacggc gcggaccgat tcctcgtcct ccaccagcag
     1921 gatgcgcccc ccgcccgacc acttgctcgg ctcctccggc ttggccacga ccggcgcctg
     1981 ggccggacca tggtggaccg ggaagtagat cgagaaacgc gtccccttgc ccacctcgct
     2041 gtcggcgaag atgaacccgc ccgattgctt gacgatgccg taaacggtgg agagacccag
     2101 gcccgtgccg cgcccctgct ccttggtggt gaaaaacggc tcgaagatct tggcaaggtg
     2161 atcgggcgcg atgccgctgc cggtgtcctc gatgatcagc ccggtatagt caccctgcgg
     2221 caggatttcg gatcgcatcg cccgcacgtc ggcgctgctc acccgccggg tggtgatggt
     2281 cagccgggct ccgtcgggct tggccccgct ctccaccttg cccatcagcg cgtcgcgcgc
     2341 attgacgacg aggttgacga tgacctgctc cagctgggtc gggtccgccc gcaccgggcc
     2401 gaggtcgcgg tcgtggcgca cctcgaagcg gatcttctcg ccgatcaggc gcttgagcat
     2461 ctggctgact tcggcaacca cgtcgggcag ctgcaacacc tgcgggcgca aggtctgctg
     2521 gcgcgagaat gccagcaatt ggcgggtcag gctggccgcg cggttggaat tggcgcggat
     2581 ctgctggatg tcgtcatagt cgctgtcgcc cggcgtatgg cgcagcagca tcaggtcgca
     2641 ggtgcccagg atcgcggtca gcacattgtt gaaatcatgg gccacgccgc ccgccaattg
     2701 ccccacggcc tgcatcttgg tcgcctgggc cacctgccgc ttgagccggg tttcctcggt
     2761 cgtgtcggtc agccccagca gcaccgccgc ctcgcccagg ccgcgcaccg tggcaaggct
     2821 cagcgaaacc gggtcctccg gctggccctt caggcgcacg gcgatatcgc cggccgccgc
     2881 cggcccttgc gcatggcgcc gcaccgcgtc cgacagcgcc gccttgtcct cgcgcacaac
     2941 aaggtcggac gggaacggtg gcggcagctc gttctcgcgg cccgccgcgc gcatgaaggc
     3001 ggcattggcg aacagcagcc gcccgtcgcg gtcggccatg gccaggccaa gcggcaattg
     3061 cgccagcagg gcttccagat gcggcacggc ggcatggccc tcggccgaac tcgcccccgc
     3121 gccgatgccc atgccggaat cgaccagcag catcagcgcc gctgccgtat cgggatcggg
     3181 cgcatgggcc gcatccgggt cgatcaccgg cacatagtac atggtcagcg ggccgccgcg
     3241 cgcgccatcg cgctcccacg aaatgcggtc atggtcgtcc tggcgcagca acgagacgaa
     3301 ttccatgccc accagcgccg ccgccggatc gccggtggcg cgcagggcaa agccgctcgt
     3361 ggcggccatg atccgcccat cggcgtcgac cat
//
LOCUS       DATNBV010000066         2565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4523367,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000066 DATNBV010000000
VERSION     DATNBV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4523367"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1799
                     /gene="acs"
                     /locus_tag="VN627_01510"
     CDS             <1..1799
                     /gene="acs"
                     /locus_tag="VN627_01510"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HXD01961.1"
                     /translation="WMRPWTKLNRCSFDEADFGIEWFADGTLNVSANCLDRHLKSNGD
                     TTAIIWESDDGQSYDALTYRQLHAEVCRWANVLKAKGVKKGDRVTIYLPMIPAAAASM
                     LACARIGAIHSVVFGGFSPEALAGRIQDCDSRLVITADAGMRGGKQVPLKANVDAAVA
                     HCPSVESVLVVRHVGNAVEMKGGRDRWWQDERQAASPECPAAEMGAEDPLFILYTSGS
                     TGKPKGVLHTTGGYLTWAAMTHHYVFDYRPGEIYWCAADIGWVTGHSYVVYGPLANGA
                     TVVMFEGVPNYPTHSRFWEIVDRHQVNIFYGAPTALRALMREGDEWVKRTSRSSLRLL
                     GSVGEPINPEAWEWYWRVVGDGRCPIVDTWWQTETGGAMITPLPGATDLKPGSASRPF
                     FGVVPQLVDAEGKVLEGAAEGNLCITQSWPGQMRTVYGDHARFFQTYFSTYKGKYFTG
                     DGCRRDEDGYYWITGRVDDVINVSGHRMGTAEVESALVAHAKVAEAAVVGFPHDIKGQ
                     GIYAYVTCNAEVEPDEALRKELVAWVRTEIGPIATPDAIHFAPALPKTRSGKIMRRIL
                     RKIAEGDVSSLGDTSTLADPGVVDTLIATRAG"
     gene            complement(1803..2441)
                     /locus_tag="VN627_01515"
     CDS             complement(1803..2441)
                     /locus_tag="VN627_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01962.1"
                     /translation="MHTARFAHSHSIGRRSRALKTSHLMGRIWRVLFGVVLVIVAAAA
                     LAMLLGGIGVGGLFITALACILVAALLLRYPRLRVPQGDDLARGSLTEVIDNAELWLE
                     TRRAVLPANAARQVDAIGVQLDGLGLQLDHANPRPEAEAEVRRLVGEHLPAIVGGYTA
                     ARRPPGDETALVDSLARIGSEIDTITRQLATGSLDDLALKARSMDFGPGELR"
BASE COUNT          424 a          811 c          946 g          384 t
ORIGIN      
        1 actggatgcg gccgtggacg aagctcaacc gctgctcgtt cgacgaggcc gatttcggga
       61 tcgagtggtt tgccgacggc acgctcaacg tttctgccaa ttgcctcgac cggcacctga
      121 agtcgaacgg cgacaccacg gcgatcatct gggagagcga cgacggccag tcctacgacg
      181 cgctcaccta ccggcagctg cacgccgagg tctgccgctg ggccaatgtg ctgaaggcca
      241 agggagtcaa gaagggcgac cgggtcacca tctacctgcc gatgatcccg gcggcggcgg
      301 cctcgatgct ggcctgcgcg cggatcgggg cgatccattc ggtggtgttc ggcgggttca
      361 gtcccgaggc gctggcgggg cgcatccagg attgcgacag ccgcctggtc atcaccgccg
      421 acgcgggcat gcgcgggggc aagcaggtgc cgctcaaggc caatgtcgat gcggccgtgg
      481 cgcattgccc gtcggtggag agcgtcctgg tcgtgcgcca tgtcggcaat gcggtggaga
      541 tgaagggcgg gcgcgaccgc tggtggcagg acgagcgcca ggcggcctcg cccgagtgcc
      601 cggcggcgga aatgggcgcg gaggacccgc tgttcatcct ctacacctcc ggttcgaccg
      661 gcaagcccaa gggcgtgctg cacaccacgg gcggctatct cacctgggcg gcgatgacgc
      721 accactacgt gttcgactac cgccccggcg agatctactg gtgcgcggcc gatatcggct
      781 gggtgaccgg gcattcctat gtcgtgtacg ggccgctcgc caacggggcg actgtcgtga
      841 tgttcgaggg cgtgccgaac tatcccaccc acagccggtt ctgggaaatc gtcgaccggc
      901 accaggtcaa catcttctat ggcgccccca cggcgctgcg cgcgctgatg cgcgagggcg
      961 acgaatgggt gaagcgcacc agccgctcca gcctgcgcct gctgggttcg gtgggagagc
     1021 cgatcaatcc cgaggcctgg gaatggtact ggcgcgtggt cggtgacggg cgctgcccga
     1081 tcgtcgacac ctggtggcag accgagaccg gcggggcgat gatcaccccg ctgcccggcg
     1141 cgaccgatct caagccgggt tcggcctcca ggccgttctt cggggtggtg ccgcagctgg
     1201 tggatgccga gggcaaggtg cttgaggggg cggccgaggg caacctgtgc atcacgcagt
     1261 cctggcccgg ccagatgcgc accgtctatg gcgaccatgc gcggttcttc cagacctatt
     1321 tcagcacgta caagggcaag tacttcaccg gcgacggctg ccgccgcgac gaggacggct
     1381 actactggat caccggccgg gtggacgacg tgatcaacgt ctcgggccac cgcatgggca
     1441 cggcggaagt ggagagcgcg ctggttgccc atgccaaggt ggcggaagcg gcggtggtcg
     1501 ggttcccgca cgacatcaag gggcagggca tttacgccta tgtcacctgc aatgccgagg
     1561 tggagcccga cgaggccctg cgcaaggagc tggtcgcctg ggtgcgcacc gagatcgggc
     1621 cgatcgccac gcccgatgcg atccacttcg ctccggccct gcccaagacc cgttccggca
     1681 agatcatgcg ccgcatcctg cgcaagatcg cggaaggcga tgtgagcagc ctgggggata
     1741 cctccacgct ggccgatccc ggcgtggtgg atacgctgat cgcgacgcgg gcgggatagc
     1801 ggctaacgca gttcgcctgg tccgaaatcc atggagcggg ctttcagcgc caggtcatcg
     1861 agcgagccgg tggcaagctg gcgggtgatc gtgtcgattt cgctgccgat ccgggccagg
     1921 ctgtcgacaa gggcggtctc atcgcccggc gggcggcgcg cggcggtata gccgccgacg
     1981 atggcgggca agtgttcgcc gacgagccgg cggacctcgg cttcggcctc ggggcgggga
     2041 ttggcatgat cgagctggag gccgaggcca tcgagttgga cgccgatcgc atcgacctgc
     2101 cgggcagcat tggctggtag tacggcacgc cgggtttcca gccacagctc ggcattgtcg
     2161 atgacctcgg tcagcgagcc gcgcgcaagg tcatcaccct ggggaacgcg caggcggggg
     2221 tagcgcagca gcagggccgc gacgaggatg caggcgagcg cggtgatgaa caggccgccg
     2281 acgccgatcc cgccgagcag catggcgagc gcggccgcgg cgacgatgac gaggacgacg
     2341 ccgaacagga cgcgccagat ccgccccatc aggtggctgg tcttgagcgc gcgcgagcgg
     2401 cggccgatcg agtggctgtg ggcgaagcgc gcagtgtgca agcgggaggg tgaaggcgca
     2461 tccatgccaa tggaacgccg ctggggagat cgcgctcccg cccgaacggg cgagctgtct
     2521 cagctgccgg tgaggcggcg gaacatgcgc cgaaggggcc cctca
//
LOCUS       DATNBV010000067         3949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3869953,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000067 DATNBV010000000
VERSION     DATNBV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3869953"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VN627_01520"
     CDS             complement(<1..673)
                     /locus_tag="VN627_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXD01963.1"
                     /translation="MRMELGTWAAAGNAPSPEGPLAIEARGLVKRFDGFTAVDGVDLS
                     VPEGAIYGVLGPNGAGKTTTLRMLLGIIDPDEGYRRVLGTLRPHEVAHAIGYLPEERG
                     LYPAMKAFEAIAFVGALRGLPLAEGRRRGRVLLEEHGLGHVADRQIRQLSKGMAQQVQ
                     ILGCLVHEPRLVVFDEPFSGLDAINQGKLEKTMRGLADKGTTVIFSTHVIAHAERLCD
                     GIAIIA"
     gene            721..1830
                     /gene="queG"
                     /locus_tag="VN627_01525"
     CDS             721..1830
                     /gene="queG"
                     /locus_tag="VN627_01525"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HXD01964.1"
                     /translation="MVNEPVTELLPRHLLADLLADLRAEAARLGFAACGIAPAAEDPL
                     AAGRLQQWLGEGAHGQMEWMAERAHHRRSPQALWPEARSVVALGMSYAPAADPLALAG
                     QPDRARISVYAQGQDYHDTVKKALKALARWLVAEAARRGLGEIGVKVFVDTAPVMEKP
                     LGQAAGLGWQGKHTNLVSREHGSWLFLGAIYTTLDFPPDASGADRCGSCDACQQACPT
                     QAFPAPYRLDARRCISYLTIEHAGPIPEQFRAALGNRIYGCDDCLAVCPWNKFAAAAA
                     ANRAFLPRAELAAPRLADLLALDDAGFRRLFSGSPIKRIGRNRFVRNCLYAAGNSDDP
                     GLLALVLPLTADPDPVVAEAALWAATRLSTEAPHD"
     gene            1823..2470
                     /locus_tag="VN627_01530"
     CDS             1823..2470
                     /locus_tag="VN627_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD01965.1"
                     /translation="MTDEPLAATLERLDRMESTEALRQLAFTYALAVDARDLDLLVSL
                     YVDDVRTADGPGRAALKAVFDKSLRQFTTSSHLVSNHLIEFIDPDNAFGLVSCRIEHE
                     VGSEWVTMVSLYHDRYERRAGRWYFRGRIPARLYATAEDDLPLGDNKLRWPGGTPMVG
                     NFHDSLPSWSAFWGEGGGAAYDAAAYDVAADAARDTPITRLRGTPKLPPAPPFFF"
     gene            complement(2507..>3949)
                     /locus_tag="VN627_01535"
     CDS             complement(2507..>3949)
                     /locus_tag="VN627_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXD01966.1"
                     /translation="WGAALLAPLAPSLATPAAAQLRPQPVPAVLSEVLLVSQSPTESR
                     FRLTVTPKVNGFATVGTDPGRPALGLALTSRGNSAVAPPGLDGLVRGIAFDQVDSVLV
                     MRFATSAPAAASAVTISDTTIEITVSSRTARASRTSDDSPAAASLPPASTPAPGEDLF
                     ELVMLKYADVSEVVGLLTDGQTVRSNDVFIPRSPAFGSGSLTGSSYAPQPSIPPPGSD
                     DRPLGQSVDSSLAVDRRLNAIWIKGSAERIARMKAMIALIDVPVDTVILETELVELTE
                     SGAKAVGLDFTNANGQIGVATVDTGKFIPPGYPPNDHDIRLTSAALQVALYAQIQKGQ
                     GKIVSKPRIAAQSGSTAKIVTGDALPILTSITLSGVNGVSQQVQYVNVGVTLQIAPRV
                     SADGYVTSHVYSVVSSVSGYSQGYPTISQREAETSASVRDGDSFVIGGLTQESSLKTR
                     RCRCWATSRCWARPSGPTGRPRDGPNSTSW"
BASE COUNT          588 a         1438 c         1292 g          631 t
ORIGIN      
        1 cggcaatgat cgcgatcccg tcgcacagcc gttcggcatg ggcgatgacg tgggtggaga
       61 agatcactgt cgtgcccttg tcagccaggc cgcgcatcgt cttttccagc ttgccctggt
      121 tgatcgcatc gagccccgag aacggctcgt cgaatacgac caggcgcggc tcgtgaacca
      181 ggcagccgag gatctggacc tgctgggcca tgcccttgga cagctggcgg atttgccggt
      241 cggcgacatg gcccaggcca tgttcctcca gcaggacccg gccccggcgg cgcccttcgg
      301 ccagcggcag gcccctcagc gcgccgacga aggcgatcgc ctcgaaggcc ttcatcgccg
      361 gatagagccc gcgctcctcg ggcaggtagc cgatggcatg ggccacttcg tgcggacgca
      421 gggtgccgag cacgcggcga tagccctcgt cggggtcgat gatccccagc agcatgcgca
      481 gcgtcgtggt cttgccggcg ccgttgggcc cgagcacgcc gtagatcgcg ccctccggta
      541 ccgacaggtc gacgccgtcc accgcggtga acccgtcgaa ccgtttcacc agcccgcggg
      601 cctcgattgc gagcgggcct tccggtgacg gggcattgcc cgccgctgcc caagtgccta
      661 gttccattct catgagtgtg accggttacc cctgcttcgt gaagaggagg atacgcgagc
      721 atggttaacg agccggtcac cgaactgttg ccccgacatc ttctggccga tcttctggcc
      781 gacctccggg ccgaggccgc gcgcctcggt ttcgccgcct gcggcatcgc gcccgccgcc
      841 gaagacccgc tcgccgccgg ccgcctccaa cagtggctgg gggagggcgc ccacgggcaa
      901 atggaatgga tggccgagcg cgcccaccac cgccgctcgc cccaggcgct ctggcccgag
      961 gcccgcagcg tcgttgcgct cggcatgtcc tatgcccccg ccgccgatcc gctggccctt
     1021 gcgggacagc ccgaccgcgc ccggatctcg gtctatgcgc aagggcagga ctatcacgat
     1081 acggtcaaga aggccctcaa ggcgctcgcc cgctggctgg tggccgaagc ggcgcggcgc
     1141 gggctggggg agatcggcgt caaggtcttc gtcgatacgg ctccggtcat ggaaaagccg
     1201 ctgggccagg ccgccgggct cggatggcag ggcaagcaca ccaacctcgt cagccgcgag
     1261 catggctcat ggctgttcct cggcgcgatc tacaccacgc tcgacttccc gcccgatgca
     1321 agcggtgcgg accgctgcgg ctcctgcgat gcctgtcagc aggcctgccc cacccaggcc
     1381 ttccccgccc cctaccggct cgatgcccgc cgctgcatct cctatctcac catcgagcat
     1441 gccgggccga ttcccgaaca gttccgcgct gcgctgggca accgcatcta cggctgcgac
     1501 gattgcctcg ccgtctgccc gtggaacaag ttcgccgccg ccgccgcggc caaccgtgcc
     1561 ttcctccccc gcgcggaact ggccgccccc cgccttgccg atctgctcgc gctggatgac
     1621 gcgggctttc gccgcctgtt ctccggcagc ccgatcaagc ggatcggccg caacagattt
     1681 gtgcgcaatt gcctctatgc cgccggcaac agcgacgatc ccggcctgct ggcgctagtc
     1741 ctgccgctca cggccgatcc cgatccggtc gtcgccgagg ccgccctctg ggcagcaacc
     1801 cggctcagca cagaggcccc gcatgactga cgaacccctc gccgccaccc tcgagcgcct
     1861 cgaccgcatg gaatcgaccg aggccctgcg ccagctcgcc ttcacctatg ccctggcggt
     1921 cgatgcgcgc gatctcgacc tgctcgtctc gctctatgtc gatgatgtgc gcaccgccga
     1981 tggcccgggc cgcgcggcgc tcaaggccgt gttcgacaag tcgctgcgcc agttcaccac
     2041 ctcgtcgcac ctcgtctcca accacctgat cgaattcatc gacccggaca acgccttcgg
     2101 cctcgtctcc tgccggatcg agcacgaggt gggcagcgaa tgggtgacca tggtcagcct
     2161 ctatcacgac cgctacgagc gccgcgccgg ccgctggtat ttccgcggcc gcatccccgc
     2221 ccggctctat gcgacggcgg aggacgacct gccgctcggt gacaacaagc tgcgctggcc
     2281 cggcggcacg ccgatggtcg gcaatttcca cgattcgttg cccagctgga gcgcgttctg
     2341 gggggagggc ggcggcgcgg cctacgatgc cgccgcctac gatgtcgccg ccgacgcggc
     2401 ccgcgacacc ccgatcaccc gcctgcgcgg cacgcccaaa ctgccgccgg cgccgccgtt
     2461 cttcttctga accctcagga tgcggtccgc ttcaccacgt gcggcgtcac cacgatgtag
     2521 agttcggtcc gtcccttggt ctcccggtcg gtccggaagg cctggcccag caccgggacg
     2581 tcgcccagca gcggcacctg cgggtcttga gcgaagactc ctgcgtgagc ccgccgatca
     2641 cgaacgaatc gccgtcccgc accgaggccg atgtttccgc ctcgcgctgg ctgatcgtgg
     2701 ggtacccttg tgaatagccc gaaacgctgg aaaccaccga atagacgtgg ctcgtcacat
     2761 agccatcggc cgaaacccgg ggcgcgatct gcagggtcac gcccacgttg acgtactgga
     2821 cctgctgcga caccccgttg acgcccgaca atgtgatcga ggtcaggatc ggcaaggcat
     2881 cgccggtcac gatcttggcg gtcgatccgc tctgcgcggc aatgcgcggc ttggagacga
     2941 tcttgccctg gcccttctgg atctgcgcat agagcgccac ctgcagcgcg gcgctggtca
     3001 ggcggatgtc gtgatcgttc ggcgggtacc cgggcgggat gaacttgccg gtatccaccg
     3061 tcgccacgcc gatctggccg ttggcattgg tgaagtcgag ccccaccgcc ttggccccgc
     3121 tctcggtcag ctcgaccagc tcggtctcga ggatcaccgt atcgaccggc acgtcgatca
     3181 gcgcgatcat cgccttcatc cgcgcgatcc gctccgccga acccttgatc cagatggcgt
     3241 tgaggcgccg gtccacggcc agcgaactgt cgaccgactg gcccagcggc cggtcgtcgc
     3301 tgcccggcgg cgggatcgat ggctggggag cataggagct ccccgtcagg ctgcccgatc
     3361 cgaaggccgg cgagcgggga atgaacacgt cattcgagcg cacggtctgc ccgtcggtca
     3421 gcaggcccac cacttcgctg acgtcggcat atttgagcat gaccagctcg aacaggtctt
     3481 cgcccggcgc tggggtggag gcgggcggca gcgatgcggc ggcgggcgaa tcgtcgctcg
     3541 tgcggctggc gcgggccgtg cgcgaggaga cggtgatctc gatggtcgtg tcgctgatcg
     3601 tcaccgcgct ggcagccgcc ggggcgctgg tggcaaagcg catgaccagc acgctgtcca
     3661 cctgatcgaa ggcaatcccg cgcaccagcc cgtccagtcc cggcggggcg acggcgctgt
     3721 tgccccggct ggtgagggcg aggccgagcg cgggccgccc cggatcggtc ccgaccgtgg
     3781 cgaaaccgtt gaccttgggg gtgacggtca gccgaaaccg gctctcggtc ggcgactggc
     3841 tgaccagcag cacttcggag aggacggcag gcaccggctg cgggcgcagt tgcgctgccg
     3901 ctggcgtggc caaggacggc gcgagcggcg ccaatagcgc cgcacccca
//
LOCUS       DATNBV010000068         2625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1959019,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000068 DATNBV010000000
VERSION     DATNBV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959019"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VN627_01540"
     CDS             <1..488
                     /locus_tag="VN627_01540"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXD01967.1"
                     /translation="SPTRVLAQLGDPARVRITATIDERDIARVTVGQAALMSSDAWPG
                     KAIPARVAEVTPGGDPSQRAFRVRLLPTTAVSLPLGLSLEINIVTRRKDRALLVPASA
                     VVGGKVWMVLEDKAHAVEVRTDVVGSEQVEVLSGITAQDMLIDKPPEGLKEGQHVKGA
                     K"
     gene            489..1763
                     /locus_tag="VN627_01545"
     CDS             489..1763
                     /locus_tag="VN627_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXD01968.1"
                     /translation="MAARALGRLNLLVHIAAKHLLVRRRQTLVAVSGVAVGVGFFLAV
                     SALMVGSQADFVKTLIDTAPHIIISDERRSPPPQPGVAAYRGNGGGGAVELHGYKVRS
                     EVRGIKDWQAILREVNDIPGAIGSPSLSGAVTLRVGGREEGLAVVGIDPGIEAKVSTI
                     SDKLRAGKLIDLERVQGGVIIGEDQAQRLALGMGDIVAATSARGGTRNLKIVGLFKRG
                     QMQLGSGTGYMLLREAQSLLGRPFVINRIGVKLADPYSAQTVAARIEARHAYKAESWQ
                     ERSADFLGLLLTRNVIMYSVVSAILLVASFGIYTAVSNSVADKRRDIAILRSIGFSQG
                     DLQLVFIIEGAALAVVGVVLGWGLGYVLMEILSRLEFPISGDVQHIPLDRGSRQYLIA
                     ATASLSAGLVAAWLPARKTALVDPVDILRGAV"
     gene            1760..2458
                     /locus_tag="VN627_01550"
     CDS             1760..2458
                     /locus_tag="VN627_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXD01969.1"
                     /translation="MTEPVLIAEKIGRTLPGDPPVTLVAEADLAIEPGKFTAIVGPSG
                     CGKSSLLYLLGLIDRPTSGKLLLDGRDVTPLSGDARARIRLERFGFVFQFHFLLPEFT
                     ALENVMLPIRRLGRLSLDKAEARARDLLGQMGLADKAGKSPDRLSGGERQRVAIARAL
                     ANDPVLVLGDEPTGNLDSVNSARVVDLFRQLAHEQGRAVVCVTHDLEIADLADIRVSM
                     LDGRISEVRRQVRS"
     gene            complement(2443..>2625)
                     /locus_tag="VN627_01555"
     CDS             complement(2443..>2625)
                     /locus_tag="VN627_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXD01970.1"
                     /translation="QRERGRGRSLPDGSELWITVHPSYLLRLRDDGREKEKARFAADL
                     AAVAERLAELAAQDRT"
BASE COUNT          399 a          928 c          872 g          426 t
ORIGIN      
        1 ccagcccgac ccgggtcctc gcccagctgg gcgacccggc ccgcgtccgc atcaccgcga
       61 ccatcgacga gcgcgacatt gcccgggtca cggtcggcca ggcggcactg atgtcgagcg
      121 acgcctggcc cggcaaggcg atccccgccc gcgtggccga ggtcaccccc ggcggcgatc
      181 cctcccagcg cgccttccga gtgcgcctcc tgcccaccac cgctgtcagc ctgccactgg
      241 gcctgagcct cgaaatcaac atcgtcaccc ggcgcaagga ccgggcgctg ctggttcccg
      301 ccagtgccgt ggtcggcggc aaggtttgga tggtgctcga agacaaggcc catgccgtag
      361 aggtgcgcac cgacgtggtc ggcagtgagc aggtggaggt tctgtccggt atcactgcgc
      421 aggacatgct tatcgacaag ccgccggaag ggctcaagga aggccagcac gtcaaagggg
      481 ccaagtaggt ggccgctcgc gccctcggcc gcctcaacct gctcgtccac atcgcggcca
      541 agcacctgct ggtgcgccgc cggcagacgc tggtggcggt cagcggggtg gcggtcgggg
      601 tgggcttctt cctggctgtc tcggcgctga tggtcggcag ccaggccgac ttcgtgaaga
      661 ccctgatcga cacggctccg catatcatca tctccgacga gcgccgcagc ccgccgccgc
      721 agcctggcgt cgccgcatac cggggcaacg gcggaggcgg cgcggtcgaa ctccatggct
      781 acaaagtccg cagcgaagtg cgcgggatca aggactggca ggcgatcctg cgcgaagtga
      841 acgatatccc tggcgccatc ggcagcccga gcctgtcggg cgcggtgacc ctgcgcgtcg
      901 gcgggcgtga ggaaggtctg gccgtggtcg gcatcgatcc cgggatcgag gcgaaggtga
      961 gtacaatctc ggacaagctg cgcgcgggca agctgatcga cctggagcga gtccagggcg
     1021 gcgtcatcat cggcgaggat caggcgcagc gactggcact cggcatgggc gacatcgtcg
     1081 ccgccacctc ggcgcgcggc ggcacgcgca atctcaagat cgtcggactg ttcaagcgcg
     1141 gacagatgca gctgggttcc ggcaccggct acatgctgct gcgcgaggcc caatcgctgc
     1201 ttgggcggcc attcgtgatc aaccggatcg gggtgaagct ggccgatccc tattccgcgc
     1261 agaccgtcgc cgcccgtatc gaggcgcgcc atgcgtacaa ggcggagtcg tggcaggaac
     1321 gctcggccga tttcctgggc ctgctgctga cccgcaacgt gatcatgtat tcggtcgtct
     1381 cggcgatcct gctcgttgcc agcttcggca tctataccgc cgtgtccaac tcggtggccg
     1441 acaagcgccg cgatatcgcc atcctgcgct cgatcgggtt ctcgcagggc gacctccagc
     1501 tggtcttcat catcgagggc gccgccttgg ccgtggtcgg cgtggtgctc ggctgggggc
     1561 tgggctatgt gctgatggag atcctcagcc ggctggaatt ccccatctcg ggcgacgtcc
     1621 agcatatccc gctcgaccgc ggaagccggc aatacctgat tgctgccacg gcctcgctat
     1681 cggccgggct ggtcgccgcc tggctgccgg ctcgcaagac cgcgctggtc gatcccgtcg
     1741 atatcctcag gggcgcggta tgacggaacc ggtcctgatt gccgagaaga tcggccgcac
     1801 cctcccgggc gatcccccgg tcacgctggt ggccgaagcc gatctcgcca tcgagccggg
     1861 caagttcaca gcgatcgtcg gcccatcggg atgcggcaag tcctcgctgc tatatctact
     1921 cgggctgatc gaccggccga ccagcggcaa gctcctgctc gacgggcgcg acgtcacccc
     1981 gctctcgggt gatgcgcgcg cccgcatccg gctcgagcgg ttcggcttcg tcttccagtt
     2041 ccacttcctc ctgcccgaat tcaccgcgct ggaaaacgtc atgctgccga tccgccggct
     2101 cgggcgcctg tcgctagaca aggctgaggc ccgcgcgcgc gacctgctgg gccagatggg
     2161 ccttgcggac aaggcgggca aatcgccgga tcggctctcg ggcggcgaac gccagcgcgt
     2221 cgccattgcc cgcgccctgg ccaacgatcc ggtcctcgtc cttggcgacg agccgaccgg
     2281 caatctcgac agcgtcaact cggcccgagt cgtcgacctg ttccgccagc tcgctcacga
     2341 gcagggccgc gcggtcgtct gcgtcacgca cgacctggag atcgccgacc tggctgatat
     2401 ccgcgtctcg atgctcgacg gacggatcag cgaggttcgg cgtcaggtcc ggtcctgagc
     2461 tgccagctct gccaaccgtt cggccacggc cgccaggtcc gccgcgaacc gcgccttctc
     2521 cttctcccgc ccgtcatccc gcagacgcag caagtagctg ggatggaccg tgatccacag
     2581 ctcggacccg tcgggcagtg accgcccccg cccccgctca cgctg
//
LOCUS       DATNBV010000069         8055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1637573,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000069 DATNBV010000000
VERSION     DATNBV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637573"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="VN627_01560"
     CDS             <1..166
                     /locus_tag="VN627_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HXD01971.1"
                     /translation="GVLKDGMLHVQAGAGIVADSVPASEQAECENKAGALFAAAREAV
                     RVASEPGFGQ"
     gene            173..772
                     /locus_tag="VN627_01565"
     CDS             173..772
                     /locus_tag="VN627_01565"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HXD01972.1"
                     /translation="MILVLDNYDSFTWNLVHYLMELGAEVEVVRNDALTAAQALETGA
                     QGFLISPGPCTPTEAGISLDLVGAAASSGRPLLGVCLGHQSIGQYFGGKVIRGGLMHG
                     KTSPVTHDGTGVFAGIPSPFTATRYHSLIVDPVPEVLAVNARSDDGHVMGFRHKTLPI
                     HGVQFHPESIATEHGHAMLANFLRLCGLKPAEVADQTLA"
     gene            781..1776
                     /gene="trpD"
                     /locus_tag="VN627_01570"
     CDS             781..1776
                     /gene="trpD"
                     /locus_tag="VN627_01570"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HXD01973.1"
                     /translation="MTDLPPVTIHPFAEDEAQHLFGLMLDGHLTDEDIARFLVDLSGR
                     GETASEITGAARAMRARMIPINAPGNAIDVCGTGGDGHHTLNVSTAVAIVVAACGVPV
                     AKHGNRAASSKAGTADTYEALGLDLARAPERAEATLNDLGICFLHAGLHHPAMARIMP
                     IRRAIGRRTIFNLLGPLANPARVRRQLVGIARPAYVPIYAEALSRLGTDKSYVVSGDE
                     GLDELSLDAGNELAEVIAGDVLMHRVTSRDAGLMQAPVAAIRGGEPAHNARALRALLQ
                     GEPGPYRDAVLFNAAAALLVADEVADWHAGVEEAAEAIDKGLANTLLDCWIAALK"
     gene            2056..2844
                     /gene="trpC"
                     /locus_tag="VN627_01575"
     CDS             2056..2844
                     /gene="trpC"
                     /locus_tag="VN627_01575"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HXD01974.1"
                     /translation="MTDKLAEICATTAAEVARRKPLRSLAELDAAARQQTAPRGFEAA
                     LRKAAIAGYGLIAEIKKASPSKGLIRNDFDPPAHARDYQAGGATCLSVLTDAPYFQGH
                     EDYLVAARAACDLPVIRKDFMVDPWQCAEARAIGADAILIIVAALSDQQMQEIEAAAL
                     ERGMDVLVEVHNPREMARAANLRSRLIGMNNRDLKRFVTDLAITEELAPLAPEGALLV
                     GESGINTHADLLRLEKAGVRCFLVGESLMREADVAAATRRLLQG"
     gene            3016..3495
                     /gene="moaC"
                     /locus_tag="VN627_01580"
     CDS             3016..3495
                     /gene="moaC"
                     /locus_tag="VN627_01580"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HXD01975.1"
                     /translation="MSQLTHLDENGKARMVDVGAKQPTAREAVASGRIRMSPQALAAI
                     RDGEVPKGDVLAAARIAGIMAAKKTAELIPLCHPLTLDSVTLDFAFEDGALRATASAR
                     LTGKTGVEMEAMTATSIALLTIYDMAKAVDKGMVIEDVRLLAKRGGKSGEWLAEPAA"
     gene            3495..4697
                     /locus_tag="VN627_01585"
     CDS             3495..4697
                     /locus_tag="VN627_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HXD01976.1"
                     /translation="MALLSLDEAQARLLALAAPLPVERVDVPGALGRYLAEPLRARRA
                     QPAAHASAMDGYAVSSADLAGPWRIVGESAAGHPFPGSPARGEAVRISTGALLPDSCD
                     TVIIQENTLREGDSLRLSGPGLAEAHRHVRHCGSDFAFEDQLLRAGTRIGPAQLALAL
                     AAGHKHLAVRRPPRIAIIDSGDELAADPERCAPHQVPASNGAMIAALVSAVPCEVRRL
                     GPVADDMDALAKALDAAEGCDVVVTTGGASVGDHDLVRPALEAWGAKLDFWKVAIRPG
                     KPLMVARRGSQLAVGLPGNPVSSMVTAYLFLMPLLRALLGAAQPLARALPFTLGGNLP
                     PVGDRREFVRAMFDEHGQLVAVRNQDSGALGSLALAEVLIDRPAGAGFAKAGDKVAAY
                     LLGNGGLA"
     gene            4806..5489
                     /gene="lexA"
                     /locus_tag="VN627_01590"
     CDS             4806..5489
                     /gene="lexA"
                     /locus_tag="VN627_01590"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXD01977.1"
                     /translation="MLTRKQHELIRFIQDRLEETGVSPSFEEMKEALDLKSKSGVHRL
                     ISALEERGFIRRLPNRARALEVLRQPDDVTAKVTAPKITAPPVQTKAPTVRAEPANDV
                     IELPLHGRIAAGVPIEALETTAILPVPAALLGPGEHYALEVSGDSMIEAGILDGDYAL
                     IRRTDSARDGEIVVALVRGEEATLKYLFREQGKVRLDPANAAYDPQFYGPGEVQVQGK
                     LAGLLRRYH"
     gene            complement(5483..7039)
                     /locus_tag="VN627_01595"
     CDS             complement(5483..7039)
                     /locus_tag="VN627_01595"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HXD01978.1"
                     /translation="MTLSRSDPLLPRMRRGLATHIHGRLAGSPGGIAAAFASGDRGGI
                     ADADEVAMRDAGLTHLLSVSGLHVSAVIGATYLLMLRLLALWPWLALRLRLPLVAAGI
                     AALAGIGYTLLTGAEVPTVRSVAGALLVLVAVALGREPLSLRLLAAAGFAVMLVWPEA
                     VIGPSFQLSFAAVLTIVAVHQAGWMRTFAARREEPWWLAGARHLGVILLTGFAIELAL
                     LPIGLYHFHRAGVYGAMANVIAIPLTTFVTMPLIALALAMDLAGAGGAAWWAVGKSLE
                     LLLALARLIAAQPGAVTTLPAMGGGSYALFLGGGLWLALWNGRVRLLGVVPLLAGVAM
                     LVMLRAPDVLVSGDGRHVGFPRLVPGRLVVLRETRSDFARDKLNEIAGMDGDVMPLEQ
                     VPEARCNADFCALEIRRGGRTWRLLLSRGADPVPLRDLAAACERVDIVIADRWLPRSC
                     HPAVLRLDRHMLEQTGGVALDLADAHIDTVAQGQGEHGWWRVPPPPGRPTEAVGAVVP
                     RQADSPPPAQ"
     gene            complement(7020..7874)
                     /locus_tag="VN627_01600"
     CDS             complement(7020..7874)
                     /locus_tag="VN627_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01979.1"
                     /translation="MGDGAKRVTTGRPPAASPDTEPLAIIPSFLSYPQMSSDAAPAGA
                     TGPMDTWSAAFVPQSGAALQQTPWRSRASLSSMLGAAEQFLADRAFDRAPWLAVGFGA
                     GIAAWFVLPMAWQWPALIGVCLAVAVVGLVMLHRDGRWPYLAQAAAGLALACAAGCGT
                     VWTKSALMGTAGIPRPVAGSFTGIVISREEQPADGRTRLVIAMRDPTVTGVAQRIVRV
                     RLNVPLELDEPAAGEGALVQVKGRLMPPGAPDVAGQLRFRAGRLVFRPCCNRNRYQPR
                     PTGDLVTF"
     gene            7801..>8055
                     /locus_tag="VN627_01605"
     CDS             7801..>8055
                     /locus_tag="VN627_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HXD01980.1"
                     /translation="MASGSVSGEAAGGRPVVTRFAPSPTGYLHIGGARTALFNWLFAR
                     HHGGKYLLRIEDTDRARSTEPAIAAIFDGLEWLGLQGDEPA"
BASE COUNT         1359 a         3027 c         2497 g         1172 t
ORIGIN      
        1 tggcgtcctc aaggacggca tgctccacgt ccaggccggc gccggcatcg tcgcggactc
       61 cgtgccggca agcgaacagg ccgaatgcga aaacaaggcc ggcgccctct tcgccgctgc
      121 ccgcgaagct gtccgggtgg ccagcgagcc gggtttcggg cagtagacac ccatgatcct
      181 cgtcctcgac aattacgaca gcttcacctg gaacctggtc cactacctga tggagctggg
      241 ggccgaggtc gaagtcgtcc gcaacgatgc gctcaccgcc gcccaggccc tggaaacggg
      301 cgcccagggc ttcctcatct cgccgggccc ctgcaccccc accgaggccg ggatcagcct
      361 cgatctcgtc ggcgcggcgg caagcagcgg gcgccccctc ctcggggtct gcctcggcca
      421 ccagtcgatc ggccagtact tcggcggcaa ggtaatccgc ggcgggctga tgcacggcaa
      481 gacctcgccc gtcacccatg acggcaccgg cgtcttcgcg ggcattccct cgccgttcac
      541 cgccacccgc taccattcgc tgatcgttga cccggtgccc gaggtcctcg ccgtcaacgc
      601 ccgctcggac gacggccacg tcatgggctt ccgccacaag acgctgccaa tccacggcgt
      661 ccagttccac ccggaatcga tcgccaccga gcatggccac gccatgctgg cgaacttcct
      721 gcgcctgtgc ggcctcaaac ccgccgaagt ggccgaccag actctggcct gacccgcggc
      781 atgaccgacc tccctcccgt caccatccat cccttcgcag aggatgaggc ccagcacctg
      841 ttcggcctaa tgcttgatgg ccacctcacc gacgaagaca tcgcccgctt cctggtcgac
      901 ctgtccggcc ggggcgagac cgccagcgag atcaccggcg ccgcccgggc catgcgcgcc
      961 cggatgatcc cgatcaatgc gcccggcaac gcgatcgacg tctgcggcac cgggggtgac
     1021 gggcaccaca ccctcaacgt gtccacggcc gtcgccatcg tcgtcgcggc ctgcggcgtg
     1081 cccgtcgcca agcacggcaa ccgcgccgcc agctccaagg ccggcaccgc cgacacctac
     1141 gaggcgctcg gcctcgatct cgcccgcgcc cccgaacgcg ccgaggcgac cctcaatgat
     1201 ctgggcatct gcttcctcca cgccggcctg caccatcccg cgatggctcg tatcatgccg
     1261 atccgcaggg caatcggccg ccgcacgatc ttcaacctgc ttggtcccct cgccaatccg
     1321 gcccgcgttc gccgccagct cgtcggcatc gcccgcccgg cctacgtgcc catctatgcc
     1381 gaggcactca gccgcctcgg caccgacaag tcctatgtcg tctcgggcga cgaagggctc
     1441 gacgagctca gcctcgatgc cggcaacgaa ctcgccgagg tgattgccgg cgatgtcctc
     1501 atgcaccgcg tcaccagccg cgatgccggc ctgatgcagg ccccggtcgc cgccattcgc
     1561 ggcggcgagc ccgcccacaa cgcccgcgcc ctgcgcgccc tcctgcaagg cgagcccggc
     1621 ccctatcgcg acgccgtcct cttcaacgcc gccgccgccc tcctcgtcgc cgacgaagtg
     1681 gccgactggc acgccggcgt cgaggaagcc gccgaggcca tcgacaaggg cctcgccaac
     1741 accctgctcg actgctggat cgccgcactc aagtaacatt tgcatgcgcc gctcctgcat
     1801 gcgggagggg ggccaaatac tcccctcccg cctgcgggaa gggctcaaat actcccctcc
     1861 cgcaggcggg agggctcaat tactcccctc ccgcaggcgg gaggggctgg gggtgggcaa
     1921 gccagcaagc gccacacctg cctgaaatac cccttctatc ctcgcctccc ttgacctgcc
     1981 gacttcaagc cctcgcgctt tcgcgcgatc caattacctt gcacccgggc gagaccatcg
     2041 gcatggcgtc ccgccatgac cgacaaactc gccgaaatct gcgccaccac cgccgccgaa
     2101 gtcgcccgcc gcaagccgct gcgctcgctc gccgagctcg acgccgccgc ccggcaacag
     2161 accgccccgc gcggcttcga ggccgccctg cggaaggccg ccatcgccgg ctacggcctg
     2221 atcgcggaaa tcaagaaagc ttcaccttcc aagggcctga tccgaaatga tttcgatccc
     2281 cccgctcatg cccgggacta tcaggcgggc ggcgccacct gcctctcggt cctgactgac
     2341 gcgccttact tccaaggcca cgaggactat ctggtcgccg cccgcgccgc ctgcgacctt
     2401 cccgtcatcc gcaaggattt catggtcgat ccgtggcagt gcgccgaagc ccgcgccatt
     2461 ggcgcggatg ccatcctgat catcgtcgcc gcactgtccg accagcagat gcaggagatc
     2521 gaggccgcgg cactggagcg cgggatggac gtgctggtcg aggtccacaa tccccgggaa
     2581 atggcccgcg cggccaatct ccgctcacgc ctgatcggca tgaataaccg cgatctcaag
     2641 cggttcgtca ccgatcttgc gataacggaa gaactcgccc cgctcgctcc ggaaggcgcc
     2701 ctgctggtcg gcgagagcgg gataaatacg cacgccgacc tgctgcggct cgaaaaagcc
     2761 ggggtgcgct gcttccttgt cggcgaaagc ctgatgcgcg aagccgatgt ggctgcggcg
     2821 acgcgccgcc tgctccaggg ttgaacggct gtcctacagg agcgcaatct ggcatgacgg
     2881 cgcgatgacg gcccgccccg cacccgcgtt tcttaaagcc cgacccggtt cgggaaacgc
     2941 cgccgccacg ggtcggtttt ttcgctcctg aaccgtgtca accgtgtcaa cttcgtaagg
     3001 aatgccgaca gtccgatgag ccagctcacc catctcgatg agaacggcaa ggcccgcatg
     3061 gtcgacgtcg gggccaagca gccgactgcc cgcgaggccg tcgcctctgg ccgaattcgc
     3121 atgtcgcccc aggcactcgc cgcgatccgg gatggcgagg tgcccaaggg tgatgtcctc
     3181 gccgccgccc gcatcgccgg gatcatggcc gccaagaaaa ccgccgagct aatcccgctc
     3241 tgccacccgc tgacgctcga ttcggtcact ctcgatttcg cgttcgaaga cggcgccctg
     3301 cgcgccacgg ccagcgcccg gctgaccggc aagactggcg tggaaatgga agcgatgaca
     3361 gcaacttcca ttgcactcct gacaatctac gacatggcca aggcggtcga caagggcatg
     3421 gtcatcgagg acgtccggct cctcgccaag cgcggcggca agtcgggcga gtggctggcg
     3481 gagcccgccg cctgatggcc ctgctctcgc tggacgaggc gcaggcgcgc ctgctcgccc
     3541 tggccgcgcc gctgccggtg gagcgggtcg acgtgcccgg agcgctcggc cgctatctgg
     3601 ctgagcccct gcgcgcccgc cgcgcccagc ctgccgccca tgcctcggcg atggatggct
     3661 atgcggtcag cagcgccgat ctcgcaggtc cctggcggat cgtcggggaa agcgccgccg
     3721 gccatccatt cccgggcagt ccggcccgtg gcgaggccgt gcgcatctcc accggcgcgc
     3781 tgctgcccga cagctgcgac acggtcatca tccaggaaaa caccctgcgc gagggcgaca
     3841 gcctgcgcct cagcggtccg ggccttgcgg aagcccaccg tcacgtgcgc cactgcggct
     3901 cggatttcgc cttcgaggac cagcttctgc gcgccggcac ccgcatcggc ccggcccagc
     3961 tcgccctcgc tcttgccgcc gggcacaagc acctcgccgt ccgccggccg ccgcgcatcg
     4021 ccattatcga cagcggcgac gaattggccg ccgacccgga gcgctgcgcc ccgcatcagg
     4081 tccccgccag caatggcgcc atgatcgctg cccttgtgtc tgccgtgccc tgcgaagtgc
     4141 gccggctcgg tcccgtggcc gacgatatgg acgccctcgc caaggcgctg gacgcggcgg
     4201 aaggctgcga tgtcgtggta accacgggcg gcgcctcggt cggcgatcac gaccttgtcc
     4261 gccccgcact ggaagcctgg ggcgcgaaac tcgacttctg gaaggttgcg atccgcccgg
     4321 gcaagccgct gatggtcgcg cggcgcggca gccaacttgc ggtcgggcta cccggcaatc
     4381 cggtctcgtc gatggtcacg gcctacctgt tcctgatgcc gctcctgcgt gccctgctcg
     4441 gcgcggccca accgctggcg cgggccctgc ccttcaccct tggcggcaac ctccccccgg
     4501 tgggcgaccg ccgcgaattc gtccgcgcca tgttcgacga gcacggccaa ctcgtcgccg
     4561 tccgcaacca ggacagcggg gcactgggct cgctcgccct tgccgaggtc ctgatcgacc
     4621 gccctgccgg agccgggttt gccaaggccg gtgacaaggt tgccgcctat ctgctcggaa
     4681 atggcggact tgcttgacga gcaagcagaa gttgcctaat tgttccgcgt tcgttcactg
     4741 aatgatgcac atgcgggccg atttcgcccc gcctggcgca ccatcgcggc gggagtgact
     4801 aggcaatgct gactcgcaag cagcatgaac tgatccggtt catccaggac cggcttgaag
     4861 aaaccggcgt ttccccctcg tttgaggaaa tgaaggaagc gctcgatctc aagtcgaagt
     4921 cgggcgtcca ccgcctgatt tccgcgctcg aggaacgcgg cttcatccgc cgcctcccca
     4981 accgcgcccg cgctctcgaa gtcctgcgcc agccggacga tgtgacggcg aaggtgacgg
     5041 cgccgaagat cacggctccg cccgtgcaga ccaaggctcc aacggtccgg gcggaaccgg
     5101 cgaacgacgt gatcgagctt ccgctccacg gccgcatcgc cgccggcgtc ccgatcgaag
     5161 cgctggaaac caccgccatc ctgcccgtcc ccgccgccct gctcggcccg ggcgaacact
     5221 acgcactgga agtctcgggc gattcgatga tcgaggcggg catcctcgat ggcgactatg
     5281 ccctgatccg ccgcaccgat tcggcgcgcg acggcgagat cgtcgtggct ctcgtccggg
     5341 gagaggaggc gaccctcaag tatctgttcc gcgaacaggg caaggtccgc ctggacccag
     5401 ccaacgccgc ctacgatccg cagttctacg ggcccggcga agtccaggtc cagggcaaac
     5461 tggcgggcct gttgcgccgc tatcactgag ccgggggcgg cgaatcggcc tgcctcggca
     5521 cgaccgctcc caccgcctcg gtcggccggc ctggcggcgg tggcacccgc caccagccgt
     5581 gctcgccctg cccttgggcc acggtgtcga tatgggcgtc ggccaggtcc agcgccacgc
     5641 cgccggtctg ctcgagcatg tgccggtcaa gcctcagcac ggcggggtgg catgacctcg
     5701 gcagccaacg atcggcgata acgatatcca cccgctcgca agccgcggca aggtcgcgca
     5761 gcgggaccgg atcggccccg cgtgacagca ggagccgcca ggtccgtcct ccccgcctga
     5821 tctccagagc gcaaaagtcg gcattgcacc gcgcctccgg tacctgctca agcggcatca
     5881 cgtcaccgtc catgccggcg atctcgttta gcttgtcccg cgcgaagtca ctgcgcgtct
     5941 cgcgcagcac taccagtcgg ccaggaacca ggcgcggaaa gccgacgtgc cgcccgtcac
     6001 ccgaaaccag cacgtctgga gcccgcagca tcaccagcat ggccactccc gccagcaacg
     6061 gcaccacccc cagcagccgc acccgcccgt tccacagcgc cagccacagc cctccgccaa
     6121 ggaacaaagc atagctcccg ccgcccatgg cggggagcgt ggtgacggcg ccgggctggg
     6181 cggcaatcag cctggccaac gccagcagca gctcaagcga cttgccgacc gcccaccacg
     6241 ccgcgccgcc cgcgcccgca agatccatcg ccagtgccag cgcaatcaga ggcatggtca
     6301 cgaaggtcgt cagcggaatg gcgatcacat tcgccatggc cccgtaaacg ccagcgcgat
     6361 ggaaatgata aagcccgatc ggcagcaggg ccaactcgat ggcgaagccg gtcagcagga
     6421 ttaccccgag gtgacgtgca ccggcgagcc accacggctc ctcccgccgc gctgcgaacg
     6481 tccgcatcca cccggcttga tggacggcca cgatcgtcag cactgctgcg aaactcaatt
     6541 ggaagctcgg tccgataacc gcttcgggcc agaccagcat caccgcgaac cccgctgccg
     6601 ccagcagccg caacgaaagc ggctcacggc cgagagccac tgccaccagc acgagcagcg
     6661 ccccggccac actgcgcacg gtcggcacct cggccccggt cagcagggtg taaccgatgc
     6721 cagccagcgc ggcgatgcct gcggcgacga gcggcaagcg taagcgaagg gcaagccatg
     6781 gccagagcgc caacaatcgc aacatgagca gataggttgc cccgattact gcgctgacgt
     6841 gcagcccgct gaccgatagc aggtgcgtca ggccagcgtc gcgcatggcc acctcgtcgg
     6901 catcggcgat gcccccccga tcgccgctgg cgaaggccgc ggcgatgccg cccggcgatc
     6961 cggcgaggcg cccgtgaata tgcgtggcga gcccgcgtcg catgcgcggc agcagcggat
     7021 cagaacgtga caaggtcacc agttggacgc ggctgatagc ggttcctgtt gcagcaaggc
     7081 cggaaaacca agcggcccgc gcgaaatcgt agctgcccgg caacatcggg ggcgccgggg
     7141 ggcatcaatc gacctttgac ctggaccagc gcgccctcgc ccgcagcggg ttcgtccagt
     7201 tccaggggca cgttgagccg cacacggaca attcgctggg ccacgccggt caccgtcgga
     7261 tcgcgcatcg cgatcaccag ccgcgttcgg ccatcggccg gctgctcctc gcgcgatatg
     7321 acaatcccgg taaaactgcc cgcgaccggc cggggtattc cggcagtccc catgagcgcc
     7381 gacttggtcc acacagtccc gcaaccggcg gcgcacgcca gagccaggcc ggcggcggcc
     7441 tgggccaggt atggccagcg cccatcgcgg tgcagcatga ccagtcccac gacagccaca
     7501 gccaggcaca cgccgatcag cgctggccat tgccacgcca tcggcaagac gaaccaggcc
     7561 gcaatccccg caccaaatcc caccgcgagc cagggggccc gatcgaaagc tcgatcggcg
     7621 agaaactgct ccgcggcccc cagcatgctg gacaagctgg cgcggctgcg ccaaggggtc
     7681 tgttgcagtg cagcgccaga ttggggaacg aatgccgcag accatgtgtc catcggcccg
     7741 gttgccccag caggtgctgc atcgctgctc atttgcggat aggacaggaa gctcggaatg
     7801 atggcaagcg gctcggtgtc tggcgaagcg gccggtggtc gccccgtcgt cacgcgtttc
     7861 gccccgtcgc ccaccggcta cctccatatc ggcggcgccc gcactgctct gttcaactgg
     7921 ctgtttgccc gccatcatgg gggcaagtac ctgttgcgca tcgaggatac cgatcgcgcg
     7981 cgctcaaccg aacccgccat cgctgcgatc tttgacgggt tggaatggct tggcctccag
     8041 ggggacgagc cggcg
//
LOCUS       DATNBV010000070        12630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3232357,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000070 DATNBV010000000
VERSION     DATNBV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 12630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12630
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3232357"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VN627_01610"
     CDS             <1..235
                     /locus_tag="VN627_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01981.1"
                     /translation="ALARSMGLAIGSHVQLLRLAGFLAYSPRNLPDGVQGPPAPTMWR
                     WRPPRHQTPVAQPERRAPPPATGAFAALAGLIR"
     gene            245..571
                     /locus_tag="VN627_01615"
     CDS             245..571
                     /locus_tag="VN627_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HXD01982.1"
                     /translation="MRLDKLLWFLRFTKTRPLAQSLAEAGHIRLNGRRVERSAQGIRV
                     GDVLVLPLPAGVRVVEITALPARRGPADEAQACYRLLDAGAEISIAPGSNQTGPHPEH
                     QGDLLP"
     gene            568..906
                     /gene="fdxA"
                     /locus_tag="VN627_01620"
     CDS             568..906
                     /gene="fdxA"
                     /locus_tag="VN627_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HXD01983.1"
                     /translation="MTYVVTDACIRCKYMDCVEVCPVDCFYEGENMLVINPNECIDCG
                     VCEPECPAEAILPDTESGLEQWLELNAKYSAEWPNLTTKRDTPADADEHKGEEGKFDK
                     YFSAEPGEGD"
     gene            1262..1795
                     /locus_tag="VN627_01625"
     CDS             1262..1795
                     /locus_tag="VN627_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HXD01984.1"
                     /translation="MVAKAHAFDVGDYVVYPKHGVGRVIELQEQEIAGMKLELYVLRF
                     EKERMTLRVPVNKVEAIGMRKLSSDKTLREALDTLKGKPKVKRTMWSRRAQEYEAKIN
                     SGDLVSIAEVTRDLFRADDQPEQSYSERQIFEAASSRLARELAAMEKTDEPAALKKIL
                     AILNEHAPKYYEAAPAA"
     gene            complement(1850..2794)
                     /locus_tag="VN627_01630"
     CDS             complement(1850..2794)
                     /locus_tag="VN627_01630"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXD01985.1"
                     /translation="MTGNNHSQIRPAGAEAIAAAARLLAAGELVAVPTETVYGLAARA
                     DSEAAVARIYRAKGRPDFNPLIVHVPGVAEAEQLARFDERAKALAHAFWPGPLTMVLP
                     LRGDAAIAPAVTAGLPTIALRCPRHPVMREVLSACALPLAAPSANRSGAVSPTTADHV
                     AASLGDKVALVLDGGATQAGIESTIIALRAEGWSLLRPGPVTAEAVAALIGPSLADIG
                     SGRIEAPGQLASHYAPGKPVRLGALDAAEDEFLIGFGPVAGAVSLSPTGDLAEAAARL
                     YACLHEAADAAQTRVAVAPVPESGIGAAINDRLRRAAA"
     gene            2851..3030
                     /locus_tag="VN627_01635"
     CDS             2851..3030
                     /locus_tag="VN627_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01986.1"
                     /translation="MPCRLPCAAGHAAITISAGTDAFGPAGGTIHFDGFAKAGIACMA
                     LAKLLYDPNAETHGS"
     gene            3027..3857
                     /locus_tag="VN627_01640"
     CDS             3027..3857
                     /locus_tag="VN627_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HXD01987.1"
                     /translation="MTGTDEGLVLARIAAALERLAPAPAPAVDWLAAPAYVWDRSGAR
                     AVPVLAAPALALLRGIDAQKQVVVANIARHAAGYAAHDMLLWGSRGMGKSALLRSGIA
                     AAQAEVGPIALVQVAPDALEGLTGLFGILAAQDRPFLVYIDDLGFEEGDSTGPRQLRS
                     WLEGGVEARPGNVRLAVTANRRAIVSRHMSEQDDPINQRDVVDDRLALADRFGLSVGF
                     HACSQDDYLAIIAGYAETYGLAFDEGDALEWSKRRGARSGRVAWQYVNELAGRAGRTL
                     "
     gene            complement(3859..5328)
                     /locus_tag="VN627_01645"
     CDS             complement(3859..5328)
                     /locus_tag="VN627_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HXD01988.1"
                     /translation="MSETKRKILVIVGLVVVLLIAGAAYVLHGNPTDRLLSATVGRTP
                     QLVDPEPEKIPTVSIPKPIGWKDGEAPEAANGLMVNRFAQGLDHPRTMLLMPNGDVIV
                     AETNRPGVPGPAGITGFVMKLMFSRAGAGEASPNKLVLLRDANGDGVAEQRFVLRSDL
                     DSPSGLAWGANKLYVADHDAVLAFDYVPGQTALTGAPRKLRDLSPGGNHWMRNLVLSP
                     DGTKLYVAVGSSSNIGENGMKAEEGRAAIWEIDVASAKGRQYAAGMRNPNGMDWNPAT
                     GELWATVQERDMLGGDLVPDYLTNVPVGVQYGWPWFYWKDQQDDRVKEPIPDYLNEYV
                     RKPEYALGAHVAALGLRFATAGNRLGPSYANGAFIARHGSWNRKPAAGYDVVFVTFDP
                     NGNPRGMPVPVLGGFLTGHGETRGRPTWIAFDRTGALLVSDDTAGIIWRVISPTAKPS
                     EGVKPVVTGHMPPQRQLNSEAVASFRPGPEDAGANGARK"
     gene            5520..6233
                     /locus_tag="VN627_01650"
     CDS             5520..6233
                     /locus_tag="VN627_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HXD01989.1"
                     /translation="MRHHATLALALLGTIAAMPALAHDGPHGAGPRMQSHWGGPFGVW
                     YGGWNGGWNGGGWQEPTVIEYTPPAAEVPGPQPQNREEWLRECRHRLGDNGVGGAVIG
                     GVVGGVAGNVIAGHGDKVLGTVAGAAVGAIAGAAIDKAEDAPRNRDRCEAMLEGWDQG
                     GGYPAGYGYGYGYGYGVPMMLVPVTMIAGAPQAARGPCKETVVTEEIITEVPRRSRLI
                     PRRVSDKRVKLVPDKRVSQ"
     gene            complement(6246..7205)
                     /locus_tag="VN627_01655"
     CDS             complement(6246..7205)
                     /locus_tag="VN627_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXD01990.1"
                     /translation="MSDDPETQAPAAEPAVPHRRKPPALGRGLGALLGEVRREESVFG
                     APATSGAAQSGLASLAVSSIEPHPEQPRRRFDEAALDELAASIAARGVIQPVIVRPLG
                     NGRYQLVAGERRWRAAQKARLHEMPALVRDLTEREVMALALIENLQRQDLNPVEEARA
                     YDRLANAEGMTQAEIARMVDKSRSHVANLQRLLGLPEPVLDLVEAGDLSMGHARALIG
                     AEDATELARAAVDKQLSVREVEKLIRRRNRPEPQAPRRAREASGSVENADILAVQNHL
                     EEFLGLSIRIAVDPDPASGTVSIRYRTLDQLDLICQRLTGGNI"
     gene            complement(7202..7978)
                     /locus_tag="VN627_01660"
     CDS             complement(7202..7978)
                     /locus_tag="VN627_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXD01991.1"
                     /translation="MIRIAIANQKGGVGKTTSAINLATALAATGWKTLLIDLDPQGNA
                     STGIGLAAADRGASSYDLLVDQVPLAACICSTRIPNLDLIPATVDLSGAEVELVGVDN
                     RTDRLRTALASEPGYDVCLIDCPPSLGLLTLNALCAADTLLVPLQCEFFALEGLSQLL
                     QTVERVQQRFNPALGIVGVALTMYDRRNRLTDQVSDDVRSCLGQLVFETVIPRNVRLS
                     EAPSHGLPALIYDHNCSGSRAYMALARELIGRLPSRRMAA"
     gene            complement(7975..8640)
                     /gene="rsmG"
                     /locus_tag="VN627_01665"
     CDS             complement(7975..8640)
                     /gene="rsmG"
                     /locus_tag="VN627_01665"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HXD01992.1"
                     /translation="MIATEAEARALVAELGEPGSLERCEHLLALLADENTRQNLVSAA
                     SLGEAWVRHIADSAQLLRNVPRETSGPWLDLGTGAGFPGLVIAALRPALPVVMVESRA
                     RRVAWLERAARELGLANSRVLGSRLELVESFRASVISARAFAPLDRLLEISARFSTAD
                     TLWMLPKGRSAWDEVNRLAGWQHMFHVEQSLTDPDAGVVVGTVSGPLAAEVQPRGKKA
                     RSR"
     gene            complement(8637..10484)
                     /gene="mnmG"
                     /locus_tag="VN627_01670"
     CDS             complement(8637..10484)
                     /gene="mnmG"
                     /locus_tag="VN627_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HXD01993.1"
                     /translation="MHQFDIIVVGGGHAGCEAAAIAARMGARVALVTFDAAQLGAMSC
                     NPAIGGLGKGHLVREVDALDGLIGRAADAAAIHYRMLNRSKGSAVQGPRVQADRKLFK
                     ASIQQQLARQPNLTVVSGEAAALHMAGQRVAGIDLADGTRLEAGAVVLCTGTFLGGTL
                     FRGEERMVGGRIGEASALRLAAQLREADLPMARLKTGTPPRLDGRTIDWAVLEEQPSD
                     LEPWTMSPLTPTRVNPQVFCAITRTNARSHDVIRENLHRSPLFTGAIGASGPRYCPSI
                     EDKIHRFGDRDGHQVFLEPEGLGTHLVYPNGISTSLPADVQLAMVRTMPGLEQVEIVT
                     PGYAVEYDHVDPRALRPSLELRALPGLYCAGQLNGTTGYEEAAAQGLVAGMQAAAAVL
                     GREPAALDRANSYMAVMIDDLTLHGVSEPYRMLTARAEYRLRLRANNAASRLTPLGLA
                     CGAIGAERAAWFERREADRALLDAALETPVSAAELARSGLAVRTDTGRLPLREWLRFT
                     GVTLEALGQWLPDDLPEDADLAAELAEDAAYAPYLVRQEGELRALRAGEALPLGDDFA
                     YARVPGLSREMVERLQRARPATLADAGRVPGITPAALAALLVQAKRQAA"
     gene            complement(10673..11950)
                     /gene="mnmE"
                     /locus_tag="VN627_01675"
     CDS             complement(10673..11950)
                     /gene="mnmE"
                     /locus_tag="VN627_01675"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HXD01994.1"
                     /translation="MTDTIYALSSGAAPAAIAVVRVSGPDAASAMLALAGKVPPPRRA
                     VTATLRGADETLLDQALVLWLPGPGTATGEDCAEFHLHGGRAVIAAVEAALATVPGLR
                     PAQAGEFTRRAFANGRIDLAEAEGLADLLAAETELQRQSALALAGGAVSRTVDDWRVR
                     VLQLSARVEAVLDFSEDDDIGGLDAGFVPDLSALTAELAEWLARPRAEVLREGFRVVL
                     AGPPNAGKSTLFNALVEAEAAITSPIPGTTRDVVTRPVAIAGVPFLFLDTAGLRPVTA
                     DPIEQTGIDRARGELTRADLVLWLGPDAECPPGAWNIEAQADRADHPAKANPRHRVSA
                     VTGEGLPALRQDLIATARAALPRPGDAALNQRQHRLLAEAHESLAAAARFADPLLIAE
                     SLRLARVAFDSLLGRTTTEDMLDALFGRFCIGK"
     gene            complement(11963..12199)
                     /locus_tag="VN627_01680"
     CDS             complement(11963..12199)
                     /locus_tag="VN627_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HXD01995.1"
                     /translation="MKVNVEVDCTPEEARRFLGLPDVSKANEAYVDAVLKAMQGTASL
                     DQLQAMSKQLAPMGEMGMALFKQFMDLGTSGKKA"
     gene            12308..>12630
                     /locus_tag="VN627_01685"
     CDS             12308..>12630
                     /locus_tag="VN627_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXD01996.1"
                     /translation="MGEYVRIATYEGDGNFPAYVARPAGAAKAAIIVIPEIFGVNPGI
                     RQKADHFASQGYLAVSPDVFWRQVEGIELDADVPEQLQRAFGLFGGHDYDAGIRDVEA
                     TIRWIR"
BASE COUNT         2036 a         4066 c         4590 g         1938 t
ORIGIN      
        1 ggccctcgcc cggtcgatgg ggctggcgat cggcagccac gtccaattgc tgcgccttgc
       61 cggattcctc gcttattcgc cccgcaacct gcccgatggc gtgcagggtc cgcccgcgcc
      121 gacaatgtgg cgctggcgcc cgccgcgcca ccagactcca gtggcccagc cggaacgccg
      181 cgccccgccg ccagccaccg gtgccttcgc cgcgctcgcc gggctgatcc ggtaggccgg
      241 cacggtgcgg ctcgacaagt tgctgtggtt cctgcggttc accaagaccc gcccgctcgc
      301 ccagagcctg gccgaggccg gccacatccg cctcaacggc cggcgggtgg agcgcagcgc
      361 gcaggggatc cgcgtcggcg acgtgctggt gctgccgcta cccgccgggg tccgggtggt
      421 ggagatcacc gctctccccg cgcgtcgcgg accggcggac gaagcccagg cctgttaccg
      481 gctgcttgac gcgggcgcag aaatttccat agcaccgggt tcgaatcaga ccggcccgca
      541 cccggagcac caaggggatc ttttgccatg acctatgtcg tcactgacgc ctgcatccgc
      601 tgcaagtaca tggactgcgt cgaggtctgc ccggttgact gtttctatga aggcgagaac
      661 atgctcgtca tcaaccccaa cgaatgcatc gactgcggtg tgtgtgagcc ggaatgcccg
      721 gccgaggcga tcctccccga taccgagagc ggcctcgaac agtggctgga gctcaacgcc
      781 aagtattcgg ccgagtggcc gaacctcacc accaagcgcg acacgccggc cgatgccgac
      841 gagcacaagg gcgaagaagg caagttcgac aagtacttct ccgccgagcc cggcgagggc
      901 gactgagcga atcaccccgg ctcgcccgag cactgctgcg gcgcggcatg ccttggctga
      961 ctgcaacgaa agctgggtaa cctactgaaa cagccgattt aacggctgga tcttccgcgt
     1021 ccttcgcaga tgcaaccacg cgggggtgga aatttcgttt caacagtgct atatcatgtg
     1081 catcggcccg gacgcatggt tcgggccagt tgctcatcac gaaaggcaag atcgcaacag
     1141 acggtagaaa agtggccgcg tgccgcttcg ccgcaaggcg aaacgcatcc cgcaaggccg
     1201 ccttccagaa ccgtccgtgc tcaccgggtt tcccggtcct tgccaggcga aaggacgata
     1261 catggtcgcc aaggcgcacg ccttcgatgt cggagattat gtcgtttacc cgaagcacgg
     1321 tgtcggccgg gtcatcgaac tgcaggagca ggaaatcgcc gggatgaagc tggagctcta
     1381 tgtgctccgc ttcgagaagg agcggatgac tctgcgcgtt ccggtcaaca aggtcgaggc
     1441 gatcggcatg cgcaagctgt cgtcggacaa gaccctgcgc gaggctctgg ataccctcaa
     1501 gggcaagccc aaggtcaagc gcaccatgtg gtcgcgccgc gcccaggagt atgaagccaa
     1561 gatcaactcg ggcgatctcg tgtcgatcgc ggaagtgacc cgcgacctgt tccgcgccga
     1621 cgatcagccc gagcagtcct attccgagcg ccagatcttc gaagcggcct cctcgcgcct
     1681 cgcccgcgaa ctcgcggcca tggaaaagac cgacgaaccg gccgcgctga agaagatact
     1741 cgcaatcctc aacgagcatg cgcccaagta ctacgaggcc gcacccgccg cctgagtgga
     1801 tttcccggtc aagaaaaaag ggccgcccgc gagggcggcc tttttcgtgt caggcggcgg
     1861 cgcggcgcag gcgatcgttg atcgcggcgc caatgccact ttccggcacc ggcgccacgg
     1921 caacgcgagt ttgggcagcg tcggccgcct cgtgcaggca ggcatagagc cgcgccgctg
     1981 cctcggcgag atcgccggtg ggtgagaggc tgacggcgcc cgcgaccggg ccgaacccga
     2041 tcaggaactc gtcctccgcc gcgtcaaggg cgcccaggcg caccggcttg cctggcgcat
     2101 agtggctggc gagctgacct ggcgcctcga tcctgcccga tccgatgtca gccaaggagg
     2161 ggccgatcag cgcggcgacg gcttcggcag tgaccgggcc ggggcgcagc agcgaccagc
     2221 cctctgcgcg aagggcgatt atagtggatt cgatccccgc ctgcgtggcg cccccgtcca
     2281 gcaccagcgc caccttgtca cccagactgg cggccacgtg gtctgccgtg gtcgggctca
     2341 cggcgccgct gcgattggcc gagggtgcgg ccagcggcag ggcacaggcg ctcagcacct
     2401 cgcgcatcac ggggtggcgg gggcagcgca gggcgatggt cggcaatccc gccgtgaccg
     2461 cgggagcgat ggcggcatcg ccgcgcaggg gcaagaccat ggtgagggga ccgggccaga
     2521 aagcatgggc gagcgccttg gcgcgttcgt caaagcgggc aagttgctcg gcttctgcta
     2581 cgccgggcac gtgcacgatc agggggttga aatcgggccg cccctttgcc cgatagatgc
     2641 gggcaacggc tgcttcgctg tcagcccggg cggcgaggcc gtagaccgtc tcagtcggta
     2701 cggccaccag ttcgcccgcg gccaacagcc gcgccgccgc agcgatggcc tccgcgcccg
     2761 cagggcgtat ctgggagtga ttgttcccgg tcatcccggc gctatagcgg tccccgggca
     2821 caagccaagc acctggaagg accggacccg atgccctgcc gtttgccttg cgccgccgga
     2881 catgccgcca tcaccatcag cgccgggacc gacgcgttcg gaccggccgg cgggacgatc
     2941 cactttgatg gcttcgccaa agccgggatc gcctgtatgg cgctggccaa gctgctttac
     3001 gaccccaatg cggagaccca cggttcatga ccggcacaga cgaaggattg gtactcgccc
     3061 gcatcgccgc tgcgctcgag cggctggcac cggccccagc gcccgcggtc gactggctcg
     3121 ctgcgcccgc ctacgtgtgg gaccggtccg gcgcgcgcgc cgtgccggtt ctcgcagctc
     3181 cggcgcttgc gctgcttcgc ggaatcgacg cgcaaaagca ggtggtcgtt gccaacattg
     3241 cccgccacgc ggccggctac gccgcccatg atatgctgct gtggggttcg cgcggtatgg
     3301 gcaagtctgc cctgctgcgc tccggcattg ccgcagccca ggcggaggtg gggccgatcg
     3361 cgctggttca ggttgcgccg gatgcgctgg aggggttgac cgggctgttc ggcatactcg
     3421 cggcgcagga ccgcccgttc ctggtctata tcgacgatct cggcttcgag gagggcgaca
     3481 gcaccggccc gcgccagctg cgctcatggc tggaaggcgg cgtggaagcg cgccccggca
     3541 acgtacggct ggcggtaacg gccaaccgcc gggccattgt cagccggcac atgtcggaac
     3601 aggacgaccc gatcaaccag cgcgacgtgg tcgacgaccg gctggccttg gcggaccggt
     3661 ttggcctgtc ggttggcttt catgcctgca gccaggacga ttatctcgcg atcattgccg
     3721 gttacgccga aacctacggt ctggccttcg acgaaggcga tgcgctggaa tggtcgaagc
     3781 ggcgcggcgc ccgttcgggc cgcgtcgcct ggcaatatgt caacgaactg gcggggcgcg
     3841 ccgggcgaac gctgtagcct actttcttgc gccgttcgcg ccggcgtctt ccgggccggg
     3901 ccggaagctg gccaccgcct cgctgttcag ctggcgctgc ggcggcatgt gcccggtgac
     3961 caccggcttg accccctcgc tcggcttggc cgtgggcgag atcacccgcc agatgatccc
     4021 ggcggtgtcg tcgctgacca gcagggcccc ggtccggtcg aaggcgatcc aggtaggccg
     4081 accgcgggtt tcgccgtggc ctgtcaggaa cccgccaagc accggtaccg gcataccgcg
     4141 cgggttaccg ttgggatcga aggtcacgaa gaccacgtcg tagcccgcgg ccggcttgcg
     4201 gttccacgaa ccatggcggg ctatgaaggc gccgttggcg tagctcggcc ccagccggtt
     4261 gccggccgtg gcaaagcgca ggccaagcgc ggccacatgg gcgccaaggg catattcggg
     4321 cttgcggaca tattcgttga ggtagtccgg gataggctct ttcacccggt cgtcctgctg
     4381 gtccttccag tagaaccagg gccagccgta ctgcactccc accggcacgt tggtgagata
     4441 gtccggcacg agatcgccgc cgagcatgtc gcgctcctgc accgtggccc agagctcgcc
     4501 agtggccggg ttccagtcca tcccgttggg gttgcgcatg cccgcggcat attgccggcc
     4561 tttggcggag gcgacgtcga tctcccagat cgcggcgcgg ccttcttccg ccttcatccc
     4621 gttttcgccg atgttcgagg atgagcccac ggcgacatag agcttggtcc cgtcggggct
     4681 gaggacgaga ttgcgcatcc agtgattacc cccgggcgag agatcgcgca gcttgcgcgg
     4741 ggcgcccgtc agcgcggtct ggcccggcac atagtcaaag gcgagcacgg catcgtggtc
     4801 cgccacgtag agcttgttcg caccccaggc gaggccggaa ggagaatcga gatcgctgcg
     4861 caggacgaag cgctgctcgg cgacaccatc gccgttggca tcgcgcagca ggacgagctt
     4921 gttgggcgag gcctcgccgg cgccggcgcg ggagaacatc agcttcatga caaagccggt
     4981 gatcccggcg ggaccgggca cgcccggccg gttggtctcc gccacgatca catcgccgtt
     5041 gggcatgagc agcatggttc gcgggtggtc gagcccttgc gcgaagcggt tgaccatgag
     5101 gccattggcc gcctcgggtg cttcgccgtc cttccagccg atcggcttgg gaatcgagac
     5161 ggtggggatc ttctccggct cgggatcgac cagctgcggc gtacgcccca ctgtggccga
     5221 cagcaaccgg tcggtcggat tgccatgcag cacataggct gcccccgcga tgaggaggac
     5281 gacgacgagg ccgacaatga cgaggatctt gcgcttggtt tcgctcatcg cgctgagata
     5341 ggccgggcgc gccatcccga caaccggcaa ggtacgaacg gtccgtcggc gcccggacgg
     5401 accgacctgc gccgaattgg gacggccggg ccgtggcggt tggtcggcgg ctgcaaccga
     5461 tgctaaattc cgggttaacg acccgggact ggcccgttcc ggacggggga aaacgaacca
     5521 tgcgccacca tgccactctc gccctagccc tgctcggcac gattgccgcc atgccggccc
     5581 tcgcccacga cggtccgcat ggcgccggcc cgcgcatgca atcgcactgg ggcgggccct
     5641 tcggtgtctg gtatggcggc tggaacgggg gctggaatgg cggcggctgg caggagccca
     5701 ccgtgatcga gtacacgccg cctgccgcag aggtgcccgg cccacagccg caaaaccgtg
     5761 aggaatggct gcgcgaatgc cgccaccgcc tgggggacaa cggcgttggc ggcgcggtga
     5821 tcggcggcgt ggtcggcggg gtcgccggca atgtgattgc cgggcatggc gacaaggtgc
     5881 tcgggacggt cgcgggcgct gccgtgggcg cgattgccgg tgcggcgatc gacaaggcgg
     5941 aagacgcgcc gcgcaaccgc gaccgctgcg aagccatgct cgaagggtgg gaccagggcg
     6001 gcggctaccc ggccggctat ggctacggtt acggctatgg ctatggcgtt ccgatgatgc
     6061 tggtgccggt caccatgatt gccggcgcac cgcaggccgc gcgcgggccg tgcaaggaga
     6121 ccgtggtgac cgaagagatc atcaccgaag tgccccgccg ctcccgcctg atcccgcgcc
     6181 gcgtttcgga caagcgcgtg aagctggtgc ccgacaagcg ggtctcccag taaaaggctg
     6241 cgaaatcaga tattaccgcc cgtcagccgc tggcagatga gatccagctg atcgagcgtg
     6301 cgatagcgga tcgagaccgt gcccgaggcc ggatcgggat cgaccgcgat gcggatcgac
     6361 aggccgagga attcctcgag gtggttctgg acggcgagaa tatcggcgtt ctcgaccgac
     6421 ccactggcct cgcgggcacg gcgcggcgct tggggttcgg gccggttgcg gcggcggatc
     6481 agcttctcga cctcgcgcac cgataattgc ttgtcgaccg cggcgcgggc cagttcggtg
     6541 gcatcctcgg cgccgatcag ggcgcgggca tggcccatcg acaagtcacc ggcctcgacc
     6601 aggtcgagca ccggctcagg caggccgagc aggcgctgga ggttggcgac gtggctgcgc
     6661 gacttgtcga ccatgcgcgc gatttcggcc tgggtcatgc cttcggcatt ggcgaggcga
     6721 tcgtaggcgc gggcctcctc gaccgggttg agatcctggc gctggaggtt ttcgatcagg
     6781 gccagggcca tgacctcgcg ctctgtcagg tcgcggacca gcgcgggcat ttcgtggagc
     6841 cgggccttct gcgccgcgcg ccagcggcgt tcgccggcga cgagctggta gcggccattg
     6901 ccgagcgggc ggacgatgac cggctggatc acgccccgcg cggcaatgga agcggcgagc
     6961 tcatcgaggg cggcttcatc gaagcggcgg cggggctgct cggggtgagg ctcgatcgag
     7021 gagacggcga ggctggcgag gcccgactgg gcggcaccgg aagtggcagg cgcgccgaac
     7081 accgattcct cgcggcggac ctcgccaagc agggcgccga gaccgcgacc gagggccggg
     7141 ggtttgcggc ggtgcgggac ggcaggttcg gccgcggggg cctgggtttc cggatcgtca
     7201 ctcatgcggc cattctccgg gacggcaggc gcccgatgag ctcgcgggca agcgccatgt
     7261 aggcgcggct gccggagcag ttgtgatcgt agatgagggc ggggagcccg tgactcggcg
     7321 cttcggacag gcggacgttg cgcgggatca cggtctcgaa caccagctgg ccgaggcagg
     7381 aacgcacgtc gtccgagacc tggtcggtca ggcggttgcg gcggtcgtac atggtgagcg
     7441 ccacgccgac gatgccgagg gcgggattga agcgctgctg gacgcgttcg accgtttgca
     7501 gcagctggct caggccttcg agggcgaaga actcgcactg gagcgggacg agcagggtat
     7561 cggcggcaca gagcgcgttg agcgtgagca ggcccagcga gggcgggcaa tcgatcaggc
     7621 agacatcgta gcccggctca ctggcaaggg cggtgcgcag gcggtcggtg cggttgtcga
     7681 cgccgaccag ctcgacctcg gcaccggaga gatcgaccgt ggccgggatc aggtcgaggt
     7741 tcgggatcct tgttgagcaa atacatgctg caagaggcac ctgatcaacc agcaggtcat
     7801 aggaagaggc cccgcggtcg gcggcggcga ggccgatgcc ggtggaggcg ttgccctggg
     7861 gatcgaggtc aatcagcagg gttttccagc cggttgcggc cagggccgtg gcgaggttga
     7921 tggccgaggt cgttttgccc acgcccccct tctgattggc aatcgcgatg cggatcatct
     7981 gctgcgcgcc ttcttccctc gcggttgcac ttcggcagca agcggaccgg acaccgttcc
     8041 caccaccacg ccagcatccg gatcggtgag cgattgttcc acgtggaaca tatgctgcca
     8101 acccgccaag cgattcactt catcccaagc cgagcggccc ttgggcaaca tccataacgt
     8161 gtcggctgtg gaaaatcgcg cggaaatctc cagcaaacgg tcgagtggag cgaaggcccg
     8221 tgccgatatg acgctggcgc gaaaagattc gaccagttcg agacgcgagc cgagcacgcg
     8281 gctgttggca agaccgagct cgcgagcggc gcgttcgagc caagctacgc ggcgcgcgcg
     8341 cgattcgacc atcaccaccg gcagggccgg gcggagggcg gcaatcacca ggccggggaa
     8401 gccagcgccc gtcccaaggt cgagccaagg cccggatgtt tcacgtggaa cattccggag
     8461 caactgggcc gaatcagcga tgtgccgcac ccaggcttca cccaggctcg ccgccgagac
     8521 gaggttctgg cgggtattct cgtcagccag caaggcgagc agatgctcgc accgctccag
     8581 cgaaccgggc tcacccagct cggcaaccag cgcgcgcgcc tcggcttcgg tggcaatcac
     8641 gcggcctgcc gcttggcctg gaccagcagc gccgccagcg ccgccggagt aatgccgggg
     8701 acccggccag cgtcggccag cgtggcaggg cgggcgcgct ggaggcgttc gaccatttca
     8761 cgcgacaggc cgggcacccg ggcataggca aagtcatcgc cgagcggcag ggcctcgccc
     8821 gcgcggaggg cgcgcagttc gccttcctgc cggacaaggt agggcgcata ggccgcatct
     8881 tcggcgagct cggcggcaag atcggcatct tcgggcaggt cgtccgggag ccactggcca
     8941 agcgcttcca gggtcacgcc ggtgaagcga agccactcgc gcaggggcaa gcggccggtg
     9001 tccgtccgta cggctaggcc actgcgggcg agttcggcgg cgcttaccgg cgtctccagt
     9061 gcagcatcga gcaaggcgcg gtcggcctcg cgccgttcga accatgccgc gcgttcggca
     9121 ccgatcgctc cgcaggcgag gccgagcgga gtcaggcggc tggcggcatt gttggcgcgc
     9181 aggcgaaggc gatattcggc gcgcgcggtg agcatgcgat agggctcgct taccccgtgc
     9241 agcgtgagat cgtcgatcat cacggccatg taggaattgg cgcgatcgag cgcggccggt
     9301 tcgcggccga ggacggcggc ggcggcctgc atgccggcaa cgaggccctg ggcggcggct
     9361 tcctcataac cggtggtgcc gttgagctgg ccggcacaat agagcccggg cagggcgcgc
     9421 agttcgaggc tgggccgcag ggcgcgggga tcgacgtgat cgtactcgac cgcgtagcct
     9481 ggcgtgacga tctccacctg ttcgagcccg ggcatggtgc ggaccatggc gagctggaca
     9541 tcggctggga gcgaggtgga gatgccgttg ggatagacga ggtgggtgcc cagcccttcg
     9601 ggttcgagga acacctggtg cccgtcgcgg tcgccgaagc ggtggatctt gtcttcaatg
     9661 gatgggcagt agcgcgggcc cgaggcgccg attgcgccgg tgaacagcgg cgagcggtgg
     9721 aggttctcac ggatgacgtc atgactgcgc gcgttggtgc gggtgatggc gcagaacacc
     9781 tgcgggttca cccgtgtcgg cgtcagcggc gacatggtcc acggctcgag gtcggacggc
     9841 tgctcttcaa gaacggccca atcgatggtg cggccatcga ggcggggcgg ggtgccggtc
     9901 ttgaggcggg ccatcggcag gtcggcctcg cgcagctggg cggcgaggcg cagggccgaa
     9961 gcttcgccaa tgcggccgcc gaccatccgc tcctcgccgc ggaacagggt gccgccaagg
    10021 aaggtgccgg tgcacaggac cacggcgccc gcttcgaggc gggtgccgtc ggcgagatcg
    10081 atgccggcga cgcgctggcc cgccatatgc aaggccgcgg cttcacccga taccacggtg
    10141 agattgggct gccgggcgag ctgctgctgg atcgaggcct tgaatagctt gcggtccgcc
    10201 tggacgcgcg ggccctgcac ggccgagccc ttcgagcggt tgagcatgcg gtagtggatc
    10261 gctgcggcat cggctgcgcg gccgatgagg ccatcgagcg catcgacctc gcgcacgagg
    10321 tggcccttgc cgaggccgcc gatcgccggg ttgcagctca tcgcgccgag ctgggcggca
    10381 tcgaaggtga ccagagcgac gcgcgcaccc atccgcgcgg cgatggccgc cgcctcgcag
    10441 ccggcatgtc ctccgcctac gacgatgatg tcgaactggt gcatgggggc cgcgcttaca
    10501 ggaattggcg gcgggggtct aggcggggga ggtgaggtgt ttcacgtgga acacttgggg
    10561 gcatggtgtc tcgactgcgc tcgacacgaa cgggtggcgg gggaagggcg gtttgggcgt
    10621 gccccgctcc accacccttt gcgcggtccc cctccccgcc cgtggggagg atttacttgc
    10681 cgatgcagaa gcggccgaac agggcgtcga gcatgtcttc agtcgtggtg cggccgagca
    10741 ggctgtcgaa ggcgacgcgg gcaaggcgca gggattcggc gatgagcagg ggatcggcga
    10801 accgggcggc ggcggcgagg ctttcgtggg cttcggcgag gaggcggtgc tggcgctggt
    10861 tgagcgcggc gtcaccggga cgaggcaggg cggcgcgggc agtggcgatc aggtcttgcc
    10921 gcaaggcggg gaggccttct cccgtgacgg ccgagacccg gtggcgcggg ttggccttgg
    10981 ccgggtgatc ggcgcggtcg gcctgggctt cgatgttcca ggcgccgggc ggacattccg
    11041 cgtcgggccc gagccagagg acgagatcgg cgcgggtcag ttcgccgcgg gcgcggtcaa
    11101 tcccggtctg ctcgatggga tcggcggtta ccgggcgcag gcccgccgtg tcgaggaaca
    11161 ggaagggcac tccggctatg gcgaccgggc gggtgaccac atcgcgggtg gtgccgggga
    11221 tcggcgaggt gatcgcggct tcggcctcga ccagagcgtt gaacagggtg gacttgccgg
    11281 cattcggcgg cccggcaagc acgacgcgaa acccttcgcg cagaacttcg gcgcgggggc
    11341 gggcgagcca ttcggccagt tcggcggtga gcgcggaaag atccgggacg aagccagcgt
    11401 cgagcccgcc gatgtcatcg tcctcggaga aatcgagcac ggcttcgacg cgggccgaga
    11461 gctggaggac acggacacgc caatcgtcaa ccgtgcggga aacggcgcct ccggcgaggg
    11521 cgagggccga ttggcgctga agctcggttt cagcggcgag gagatcggcc aggccttccg
    11581 cctcggccag atcgatccgg ccgttggcaa aggcccggcg ggtgaattcg cccgcctggg
    11641 ccgggcgcag gccggggacc gtggcgagcg cagcctcgac cgccgcgatc accgcgcggc
    11701 cgccatggag gtggaactcg gcgcagtctt ccccagtggc ggtgccgggg ccggggagcc
    11761 agaggaccag ggcctggtcg agcagggtct cgtccgcacc gcgcagggtg gcggtgaccg
    11821 cgcggcgggg cggggggacc ttgccggcca gggccagcat ggcgctggcg gcatcggggc
    11881 cgctgacccg gacgacagcg atggccgcgg gggcagcgcc gctggacagg gcgtagatcg
    11941 tatcggtcat gcaggggtga ggtcaggcct tcttgcccga ggtgcccaga tccatgaact
    12001 gcttgaacag ggccatgccc atttcgccca tcggcgcgag ctgcttgctc atggcctgga
    12061 gctgatcgag gctggcggtg ccctgcatgg ctttgagcac ggcgtcgaca taggcctcgt
    12121 tggccttgct cacatcgggg aggccgagaa agcggcgagc ctcttcgggg gtacaatcga
    12181 cttcgacatt gaccttcatc gctcagcctc ccttgcgcat gccggcaagg ctggcacagg
    12241 gccttggcaa agtccaccgg acgggcaatc ctgccggctg gaccgggaat cgagcggaga
    12301 gcgagaaatg ggtgaatatg tgcgcatcgc aacctacgaa ggcgacggga atttcccggc
    12361 ctatgtggcg cggcccgcgg gcgcggcgaa ggctgcaatc atcgtcatcc ccgagatctt
    12421 cggggtgaac cccggcatcc gccagaaggc cgaccatttc gccagccagg gatatctcgc
    12481 cgtctccccc gacgtgttct ggcggcaagt ggaagggatc gagctggatg ccgatgtacc
    12541 cgaacagctc cagcgggcgt tcggcttgtt tggcgggcat gattacgatg ccggcattcg
    12601 cgacgtcgag gcgaccattc ggtggatccg
//
LOCUS       DATNBV010000071         2099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2601308,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000071 DATNBV010000000
VERSION     DATNBV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601308"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1761
                     /locus_tag="VN627_01690"
     CDS             88..1761
                     /locus_tag="VN627_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01997.1"
                     /translation="MIAGRSTAMLAPALLLAAMPGAMGQVAPQPANPAREAPSPSVRT
                     GVTTHFSQGWPDRLMADAARLGAVTIRDSVAWPRVEREAGRYEFTAANSGHIARACAA
                     GMTVLLGLEPRNPLYDAGQTVWSPAGQSAFARYVRALADRWPGCVIAVEIGNEINGKG
                     GMTGPATANRVAAHVALLKAVHSAVKPAHPEVSLLGGSVNTVATGFLSRLFAAGALDW
                     VDGIAVHPYRSEPEGLDRELARVTAAMHRAGRLRPIWATEFSREFPDPAAAPGWYLKA
                     AALMESAGVSAHFWYALADQPAFPTMGLERLDSTAKPVARAFAFAARELAPLGPARRV
                     DHGDPTLFHFRYGRDTQVIWGAARRLSAPPGARAFAADGTPIAMPARLGETPVVIRQA
                     PAIGFGPSDILADSALGFGEPPLRWFARKADGALLPLAPVDWEWTTYLGTPALPAFAA
                     TASGIGTTATAGTLVRYTAPTGPPMRVTAALCLRPCIPRPAVHATIMSGSRTLWEAPL
                     TGPARLAVPATLVAGEHLDLVLMPARGAAPARIPYRLQIVRGDAPAPPC"
     gene            1802..>2099
                     /locus_tag="VN627_01695"
     CDS             1802..>2099
                     /locus_tag="VN627_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01998.1"
                     /translation="MTPTLLGLIFFLVGLIVMLTGSRQDMLAYVMGCALFNGSASAVL
                     TALGNTSVQPAVVATGLLALRCILPDRKRPSLIAASLSANAWLLLFVGWSFIGAF"
BASE COUNT          261 a          851 c          656 g          331 t
ORIGIN      
        1 cgaacctgcc gccgccgtcg ccaatgccgc ccgcttcgcg ccggaacggt tcgatgcggc
       61 ctttcgcgcc gtcgtggagc aggcgctgtg atcgcggggc gtagcacggc catgctcgcg
      121 ccggcgttgc tgctggcggc catgcccgga gcgatgggcc aagtcgcccc ccaacccgcc
      181 aaccccgcgc gggaagcccc atcgccttcg gttcgcaccg gcgtcaccac ccatttcagc
      241 cagggctggc ccgatcgcct gatggccgat gccgcgcggc tgggcgcggt gacgatccgc
      301 gattccgtcg cctggcccag ggttgagcgc gaggccgggc gctatgaatt caccgccgcc
      361 aattccggcc acatcgcccg cgcctgcgcc gccgggatga ccgtcctgct cgggctggag
      421 ccgcgcaacc cgctctacga tgcggggcaa accgtctggt cgcccgccgg gcagagcgcc
      481 ttcgcccgct atgtccgcgc cctcgcagat cgctggccgg gctgcgtcat cgccgtggaa
      541 atcggcaatg agatcaacgg caagggcggg atgaccgggc ctgccaccgc caaccgcgtc
      601 gccgcccatg ttgccctgct caaggccgtt cacagcgccg tcaaaccggc ccatcccgag
      661 gtttccctcc tcggcggctc ggtcaatacc gtggccaccg gctttctttc ccgcctgttc
      721 gccgccggcg cgctggactg ggtggatggc atcgccgtcc acccctaccg atccgagcct
      781 gaagggctgg accgcgaact ggcacgggtg acagcggcca tgcaccgcgc cggccgcctg
      841 cggccgatct gggcaacgga attcagccga gagtttcctg atccagcggc agcacccggc
      901 tggtacctca aggccgccgc cctgatggaa agcgccggtg tatctgcgca tttctggtat
      961 gccctggccg atcagcccgc cttccccacc atggggctcg aacggctgga tagcacggcc
     1021 aagccggtcg cccgcgcctt cgccttcgcc gcgcgcgaac tcgccccgct cggcccggcc
     1081 cggcgggtgg accatggcga tccgaccctg tttcacttcc gctacggccg cgatacccag
     1141 gtgatctggg gcgcggcacg ccgcctgtca gcccccccgg gcgcccgcgc cttcgccgcg
     1201 gatggcaccc cgatcgccat gcccgcgcgg ctgggcgaaa cgcccgtggt gatccgccag
     1261 gcgcccgcca tcggctttgg cccgtccgac atcctggccg atagtgccct cggcttcggc
     1321 gagccgccgc tccgctggtt tgcccgcaag gccgacggtg ccctgctccc gctcgcgccg
     1381 gtggactggg agtggaccac ctatctcggc acccccgccc tccccgcctt cgccgccacg
     1441 gccagcggga tcggcaccac ggccacggcg ggcacgctgg tgcgctatac cgcacccacc
     1501 ggcccgccca tgcgcgtcac cgcggcactc tgcctccgcc cctgtatccc gcgcccggcc
     1561 gtccatgcca cgatcatgag cggcagccgc accctgtggg aggccccgct caccggcccc
     1621 gcacgcctcg cggttcccgc cacgctcgtt gccggggaac atctggacct cgtcctgatg
     1681 ccggcccgcg gagctgcccc ggcccgcatc ccctaccgcc tgcagatcgt ccgcggcgat
     1741 gcccccgccc cgccatgctg atcctccccg cccccttccc tcccgcaacg gaccgcgcgc
     1801 gatgaccccg accctgctgg gcctgatatt cttcctcgtc ggcctgatcg tgatgctgac
     1861 cggctcacgc caggacatgc tggcctatgt catgggctgc gccttgttca acggctcggc
     1921 cagcgcggtg ctgaccgcgc tcggcaatac gtcggtccag ccggcggtgg tcgccaccgg
     1981 cctactcgcc ctgcgctgca tcctgcctga ccggaagcgg cccagcctga tagccgccag
     2041 cctctcggcc aatgcctggc tgctgctgtt cgtcggctgg tcattcattg gggccttca
//
LOCUS       DATNBV010000072         4321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_684749,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000072 DATNBV010000000
VERSION     DATNBV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684749"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VN627_01700"
     CDS             <1..272
                     /locus_tag="VN627_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD01999.1"
                     /translation="AIGERIGACWKGPDAPDAPAVLLKLTLAQDGSVGAVETIEKKRF
                     AAEPAYRASATAATRAVLQCAPFSLPAADFAAWKSLALKLTPRHA"
     gene            310..1611
                     /gene="tolB"
                     /locus_tag="VN627_01705"
     CDS             310..1611
                     /gene="tolB"
                     /locus_tag="VN627_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HXD02000.1"
                     /translation="MPAAIAAFLLIAFAAAAARAELRIDITQGTVQPMPIAITDFYGQ
                     QPNDAQSGVNIAGVVSADLERSGLFKPIDKKAFIQTPDSLRTMPRFADWRVINAQALV
                     SGTVETQPDGRLRVEFRLWDVFAEQQMVGEAVTSTQENWRAIAHKVADEIYKRLTGEE
                     GYFETRIVYVAETGAFTNRVKRLAIMDQDGENNRYLTDGSNLVLTPRFSPSSQDITYL
                     SYRNNRPRVYLLNIETGQQEVLGDFAGMTFAPRFSPDGTKVVMSLSTNGNSDIYEMDL
                     RTRKVRRLTDTPAIDTGPSYSPDGTQIAFESDRGGSQQIYVMNADGSNQHRISFGEGK
                     YGTPVWSPRGDQIAYTHIKGGVFYIGVMHPDGSGERSITQGYLVEGPTWAPNGRVLAY
                     FKQAGPGGQVRLYSIDVTGFNERELVTPTDASDPAWSPLIQ"
     gene            1790..2272
                     /gene="pal"
                     /locus_tag="VN627_01710"
     CDS             1790..2272
                     /gene="pal"
                     /locus_tag="VN627_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXD02001.1"
                     /translation="MAATAMLLLAACSSKPADTGAANTTGTTAPAATATVPQGPAAGS
                     IEEFTTTIGDRVFFGFDRYDLTAEAQAQLQKIAAWLKTYPQYRFVIEGHTDDRGTREY
                     NLALGERRANATKNYLIALGIDQNRMQTISYGKERPVCTESTESCWAQNRRAVVVLQQ
                     "
     gene            2378..3541
                     /gene="ybgF"
                     /locus_tag="VN627_01715"
     CDS             2378..3541
                     /gene="ybgF"
                     /locus_tag="VN627_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HXD02002.1"
                     /translation="MYRCAAPYFRRQDGTACPNRRVSLPPDTERDPMTKFPAALPPLR
                     LALSLAWLSLAVLLAGAPIARAQDDVQALSQKVDRLQQQLADLERQVYNGQAPAAGGG
                     AAPTGDVAANHEVRLQQLESQMSTLTGQIEELRNAIQQLSARMDKMSQDTDFRLSALE
                     HGQPAGAAAAGAAAATVAGTGAPSAAAQPPLQNLQQIQPMQPLQQVDPNQPLKQSSLA
                     PATLGTLTQSQLQSANVQPAPGTAAAQAAATPSGGTIPLPGDSVEAQYEYAFDLLRQA
                     KYDDAEKGFRSFLVKHPNDYLAGNAQYWMGETYYVRGRYQDAAVTFAEGYQKYPTNSK
                     APDNLLKLGMSLGQLGKKADACVAFTELTKKFPTAPDSVMTRLKEEKRRYACR"
     gene            3642..>4321
                     /gene="tilS"
                     /locus_tag="VN627_01720"
     CDS             3642..>4321
                     /gene="tilS"
                     /locus_tag="VN627_01720"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXD02003.1"
                     /translation="MAALGPFEDRPTIAAAVSGGADSMALILLLERWARARGGSAIAL
                     TVDHRLRPEAAAEARQVAAWLTARGIAQRSLTWEHHGSGPRSALQAKARRERYRLLTG
                     WCRRRGILHLALAHHAGDQAETVLMRLARGAGIDGLAGMSTSLAREGVRLIRPLLLTD
                     PRRLRASLAAAGQAWLEDPSNRDERFERVRWRQLVPPEQRVPVALAAAEIGQERRRRE
                     HDLADLLSR"
BASE COUNT          747 a         1490 c         1370 g          714 t
ORIGIN      
        1 cggccatcgg cgagcgcatc ggcgcctgct ggaaggggcc ggacgccccc gatgccccgg
       61 cggtgctgct caagctgacc ttggcccagg acggctcggt cggcgccgtg gaaaccatcg
      121 aaaagaagcg cttcgcggcg gagcccgcct atcgcgcctc tgcaaccgcc gccacccgtg
      181 cggtgttgca atgcgcaccc ttctcactgc cggcggcgga ttttgccgcc tggaagtcgc
      241 tggcactcaa gctcaccccc cgtcacgcct agagaaaatc gaggtcatcc catgtttcgg
      301 ttgaaccgcc tgccggccgc catcgcggcc ttcctcctca ttgctttcgc cgccgccgcc
      361 gcgcgggccg agcttcgcat cgacatcacc cagggcacgg tgcagcccat gcccatcgcc
      421 atcaccgatt tctatggcca gcagcccaat gacgcccagt ccggcgtcaa tatcgccggg
      481 gtggtctcgg cggatctcga gcgttccggg ctgttcaagc cgatcgacaa gaaggccttc
      541 atccagacac ccgattcgct gcgcaccatg ccgcgcttcg ccgattggcg cgtgatcaat
      601 gcccaggccc tggtcagcgg cacggtggag acccagcccg acggcaggct tcgggtcgag
      661 ttccggctct gggacgtctt cgccgagcag cagatggtcg gcgaggcggt caccagcacc
      721 caggagaact ggcgcgccat cgcgcataag gtggcggatg agatctacaa gcggctgacg
      781 ggcgaggagg ggtatttcga gacccgcatc gtctatgtcg cggagacggg cgccttcacc
      841 aaccgcgtca aacgcctcgc catcatggac caggacggcg agaacaaccg gtatctgacc
      901 gacggcagca atctggtgct gaccccgcgc ttctcgccca gcagccagga catcacctat
      961 ctcagctatc gcaacaaccg gccgcgggtc tatctgctca atatcgagac gggacagcag
     1021 gaggtgctgg gcgatttcgc cggcatgacc ttcgcgccgc gcttctcacc cgacgggacg
     1081 aaggtggtga tgagtctctc cacgaacggc aatagcgaca tctacgagat ggatctgcgc
     1141 acccgcaagg tgcgccgcct gaccgacacc ccggccatcg ataccggccc ctcctattct
     1201 ccggacggga cccagatcgc cttcgaatcc gaccgcggcg gctcgcagca gatctatgtc
     1261 atgaatgccg acggcagcaa tcagcaccgc atcagcttcg gtgaaggcaa gtatggaacc
     1321 ccggtctggt cgccccgcgg agaccagatc gcctataccc atataaaggg cggagtcttt
     1381 tatatcggtg tcatgcatcc agatggctcc ggagagcgtt caattacgca gggctatctc
     1441 gtcgaaggcc ctacctgggc acccaatggt cgcgtcctcg cttatttcaa gcaggcgggt
     1501 ccgggcggcc aggtccggct ctacagcatc gacgtgacgg gcttcaacga gcgtgagctg
     1561 gtgacgccga cggacgcctc cgatcctgcc tggtcacccc tgattcagta gaggtggtct
     1621 tgattatcaa gagattgctg ggtaagattg ttgcagcgat acaaggtttc accctccggt
     1681 cggggcactt cgaccggatg gattgtcgcg acaattgccc agtctaggct atgacttccc
     1741 tcccatgggt cttggttcga aaggttcgaa gatgcgattg aaagttgtaa tggcggccac
     1801 ggcgatgctt ctgctggccg cctgctccag caagccggca gataccggcg ctgcgaacac
     1861 caccggcacg acggctcctg cggcgacggc gacggtcccg cagggcccgg ctgccggctc
     1921 catcgaagag ttcaccacca cgatcggcga tcgggtcttc ttcggcttcg accgttacga
     1981 tttgacggcc gaggctcagg cccagctgca gaagatcgcc gcttggctga agacctatcc
     2041 gcagtatcgt ttcgtgatcg aaggccatac cgacgatcgc ggcacgcgtg agtacaacct
     2101 cgccctcggc gagcgtcgtg cgaatgcgac caagaactat ctgatcgcac tcggcatcga
     2161 tcagaaccgt atgcagacga tcagctacgg caaggaacgc ccggtttgca ccgagagcac
     2221 cgaatcctgc tgggcgcaga atcggcgcgc cgtcgtcgtc ctgcagcagt aatcgctgcg
     2281 acggattgct acgagttgcg gcgcctgtcg ggacgcttcg gcggaaccgg ctggcgccgc
     2341 agccgtttta tagtctcgcc ggctgattgg actgttgttg taccggtgcg ccgcgcctta
     2401 ttttcgccgc caagacggta cagcttgtcc gaatcgccgc gtttctctac ccccggatac
     2461 cgagcgtgat ccgatgacaa aattccccgc cgccctgccg ccccttcgcc tcgcgctttc
     2521 gctcgcctgg ctgtcgctcg ccgtcttgct tgccggcgcc ccgatcgcgc gggcgcagga
     2581 cgatgtccag gccctctcgc aaaaggtcga tcgcctgcag cagcagctcg ccgacctcga
     2641 gcggcaggtc tacaacggcc aagcgccggc cgccggtggc ggggcagcgc ccaccggcga
     2701 tgtcgccgcg aatcacgagg tccggctgca gcagctcgag agccaaatga gcaccctgac
     2761 gggccagatc gaggagctgc ggaacgcaat ccagcagctt tccgcccgca tggacaaaat
     2821 gtcgcaggac accgacttcc gcctgagcgc cctggagcac gggcagccgg cgggtgcggc
     2881 tgcggccggt gccgccgccg cgacggttgc gggcacgggg gcgccgagcg ccgccgctca
     2941 gccgccgctg cagaacttgc agcagatcca gccgatgcag cccctgcaac aggtcgatcc
     3001 caatcagccc ttgaagcagt cgtctttggc acccgcgacc ctggggactc tgacccaaag
     3061 ccaattgcaa tcggcgaatg tccagccggc gccgggtacg gcagccgcgc aagccgcggc
     3121 cactcccagc ggcgggacca ttccgctgcc cggcgattcg gtggaggccc agtacgaata
     3181 tgccttcgac ctgttgcgcc aagccaaata cgacgatgcg gagaagggct tccggtcctt
     3241 cctggtcaag catcccaatg attatctggc gggcaatgcc cagtactgga tgggcgagac
     3301 ctattacgtc cgcggccgct atcaggatgc ggcggtgacc ttcgccgagg gctatcagaa
     3361 atatcccacc aacagcaagg cgccggacaa tctcctgaag ctcggcatgt cgctgggcca
     3421 gctcggcaag aaggccgatg cctgcgtggc cttcacggag ctgaccaaga aattcccgac
     3481 cgcgccggac agcgtcatga cgcgcctcaa agaagagaag cgccgctacg cctgccgctg
     3541 accccccagc ggctgtttca ccccgggggg ctgttccacc ccggggggct gctccgctcc
     3601 ggggggccgc accgatccgg gcagccgaat tcgcccggct gatggcggcg ctgggtccct
     3661 tcgaggatcg ccccaccatc gccgccgccg tttccggcgg ggccgacagt atggccctga
     3721 tcctgctcct cgaacgctgg gcccgcgcgc gcgggggtag cgccatcgcc ctcacagtcg
     3781 atcatcgcct gcggcccgag gcggcggccg aggcccgtca ggtcgcggcc tggcttaccg
     3841 cccgcggcat cgcccagcga agcctgacct gggagcacca tggcagcgga ccccgctcgg
     3901 ccctgcaggc gaaagcccgg cgagagcgct atcggttgct caccggctgg tgccgccgcc
     3961 gggggattct gcatctggcc ctcgcccatc atgccggcga ccaggcggag accgtgctca
     4021 tgcggctggc gcggggtgcc gggatcgacg ggctggcggg catgagcacg agccttgccc
     4081 gggagggggt gcgcctcatc cggccgctgc tgctgaccga tccgcgccgc cttcgggcga
     4141 gcctggcggc ggccggtcag gcgtggctcg aggatccctc caaccgggac gagcgcttcg
     4201 aacgggtgcg ctggcgccag ctggtaccgc ccgagcagcg agtccccgtc gcgctggcgg
     4261 ccgccgagat cggccaggag cggcgccgcc gcgagcatga cctggccgat ctcctgtccc
     4321 g
//
LOCUS       DATNBV010000073         4644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_55495,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000073 DATNBV010000000
VERSION     DATNBV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55495"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /locus_tag="VN627_01725"
     CDS             complement(<1..1660)
                     /locus_tag="VN627_01725"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HXD02004.1"
                     /translation="MSDGVGKKASDLFIECLEAEGCEYIFGVPGEENLDFLDSLSRSS
                     KIKLILTRHEQGAGFMAATYGRHTGKAGVCIATLGPGATNLVTAAAYATLGGMPMLMI
                     TGQKPIKKSKQGRFQILDVVAMMGPITKFTHQMASSDNIPSRVREAYRLAEEEKPGAT
                     HIELPEDIADEHTESRPLPRSSVRRPAADVKSIVQAVEALQNAKSPVLVIGAGANRKM
                     TGKMLREFVEQTGIPFLTTQLGKGVIDERHPLFLGCAALSAGDFVHRAIEDSDCIVNI
                     GHDVIEKPPFFMREGGPTVIHISTKTAEVDPVYFPQIEVIGDIANAIWQIREAITPNP
                     AWAFDHMLGYRRAEVAHTDGLSGDSRFPVFPPHLVQQVRAAMPDDGIICLDNGVYKIW
                     FARGYCAHQPNTVLLDNALATMGAGLPSAMMSAMLYPERKVMAICGDGGFMMNSQEME
                     TAVRLGLNLTVLILNDSAYGMIRWKQANMGFSDFGLTYNNPDFVKYAESYGAIGHRVE
                     SAAHLKELLAHCRDTPGVHLIDCPVDYSENDQILNKDIKVLSKAL"
     gene            1925..2536
                     /locus_tag="VN627_01730"
     CDS             1925..2536
                     /locus_tag="VN627_01730"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HXD02005.1"
                     /translation="MLGKPPPHVIIDGFLGPELHGALLDWALTGERRFSCSQVQGEDG
                     LVHNAGVRQSWHCDDGLGPLKAQFRASIDTARETIRQQLGIPPFAVVRTELELAAHRD
                     GSFYKPHVDTHVGAGRASTDGDRMLTLVYYLHRQPRGFSGGEIALYPFNLAAPTLIQP
                     ADNRLIAFPAHALHEVRPVAVPGDAWEDARFAINCWYNRERPV"
     gene            complement(2567..4024)
                     /gene="cysS"
                     /locus_tag="VN627_01735"
     CDS             complement(2567..4024)
                     /gene="cysS"
                     /locus_tag="VN627_01735"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXD02006.1"
                     /translation="MTEAQAPLVLYNSLTRAAEPFQPVHAGEARVYTCGPTVYNYPHI
                     GNMRSYVFADVLGRTLSFKGYRLTHVINITDVGHLTDDADAGEDKMEKMARAEARSIW
                     DIAEHYKQAYWADIRALNIRQPAKWTVATDYVPQMIEFARGIAEKHCYELDSGLYFDV
                     STVADYGRLARAQTDEGEGRIEAVEGKRNGADFAIWRKTPPGEKRQMEWDSPWGRGAP
                     GWHLECSVMSGEVLGFPFDIHTGGIDHREIHHPNEIAQNQAHCCTNGLDQPANSGARV
                     WMHNNFLVERSGKMSKSSGEFLRLQLLIDKGYHPLAYRLMCLQAHYRSELEFSWEGLG
                     AALTRLKRMVMQVANLRAQGEPAPAPGLKFAALLERFDAAISDDLNTSVALTVLDEAI
                     ALKKVDAGEKLAAIAAMDAVLGMGLLELGRADLRLRPKAATIAEADIEQSLTARKEAR
                     AAKDFARSDALREELAARGVEVMDGDPLGWEWKLA"
     gene            complement(4079..>4644)
                     /locus_tag="VN627_01740"
     CDS             complement(4079..>4644)
                     /locus_tag="VN627_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXD02007.1"
                     /translation="SGCNFQTWEATVLTGEPLKALQAVLLPAEVDDPLEYRYDKPNAS
                     PRHLARVQSLGAAMYGATGVDRSDAAGRAEFTRQNKISFGAPVLILSHFPKLVGEAQW
                     SDIGMWLQTVMLLAREAGLDTCPQEYMGLYGRTIKAHLGLSDDTLLFCGLALGYRDET
                     AAVNNFTRPRAELEEQVRFLGFEAVPA"
BASE COUNT          776 a         1492 c         1508 g          868 t
ORIGIN      
        1 agagcgcctt cgacaggacc ttgatgtcct tgttcaagat ctggtcgttc tccgaatagt
       61 caaccggaca atcgatgagg tggacgccgg gcgtgtcgcg gcaatgggcc agcaactcct
      121 tgaggtgtgc ggccgattcc acccggtggc caatggcgcc gtagctttcg gcgtatttga
      181 cgaaatcggg attgttgtag gtcagcccga aatcggagaa acccatgttg gcctgcttcc
      241 agcggatcat gccataggcg ctgtcgttca ggatcagtac ggtaaggttg aggccgagac
      301 ggacagcggt ctccatctcc tggctgttca tcatgaaccc gccgtcgccg caaatcgcca
      361 tgaccttgcg ctcgggatag agcatggcgc tcatcatcgc cgagggcagc cctgcgccca
      421 tcgtcgccag cgcattgtcg agcaggacgg tgttgggctg atgggcacaa tagccgcggg
      481 cgaaccagat cttgtagacc ccgttatcga ggcagatgat cccgtcgtcc ggcatggcgg
      541 cgcggacctg ttggaccagg tggggcggga acaccggaaa gcgggaatcg cccgaaagcc
      601 catcggtatg ggcaacctcg gcacggcgat agccgagcat gtgatcaaag gcccaggccg
      661 ggttcggagt gatcgcctcg cggatctgcc agatggcatt ggcgatatcg ccgatcacct
      721 cgatctgcgg gaaatagacg ggatcgacct cggccgtctt ggtcgaaatg tgaatcacgg
      781 tcgggccgcc ttcccgcatg aagaacggcg gcttctcgat cacgtcatgg ccgatgttga
      841 cgatgcagtc ggagtcctcg atcgcgcggt ggacgaaatc gcccgccgaa agcgcggcgc
      901 agcccaggaa cagcgggtgc cgttcgtcga tcacgccctt gccgagctgg gtcgtgagga
      961 agggaatgcc ggtctgctcg acgaactcgc gcagcatctt gccggtcatc ttgcggttgg
     1021 cccccgcgcc gatgaccagc acgggcgact tggcattctg cagcgcctcg accgcctgga
     1081 cgatcgactt cacgtcggcc gcggggcggc gcacggagct gcgcgggagc gggcggcttt
     1141 cggtatgctc gtcagcgata tcctcgggca gctcgatatg ggtggcgccg ggcttttcct
     1201 cctcggccag acgataggcc tcgcgcaccc ggctggggat attgtccgac gaggccatct
     1261 ggtgggtaaa cttggtgatc ggccccatca tggcaaccac atcgaggatc tggaaccggc
     1321 cctgcttcga tttcttgatc ggcttctgcc cggtaatcat cagcatcggc atcccgccga
     1381 gcgtggcata ggcggcggcg gtgaccaggt tcgttgcccc cggcccgagc gtggcgatgc
     1441 acacgccggc cttgccggtg tggcgcccgt aggtcgccgc catgaaccct gcgccctgct
     1501 cgtggcgggt gaggatcagc ttgatcttgc tggagcggga gagcgaatcg aggaagtcga
     1561 gattctcttc gccgggcacg ccgaagatgt attcacaccc ttcggcctcc aggcattcga
     1621 tgaacaggtc agaagctttc ttgcccacac cgtcgctcat gccgttcact cccaacggaa
     1681 aattgaacca gcgaaccggc taggcccggc accgtccgca cgtttccagt aaatcccgcc
     1741 agccgcttct tatcggcccc cgaccagcgc ggatgccaat gcgtaacagc atggcgaggg
     1801 caagtcaccc gggatcggct tcacgcaagc gacgccaaga aatactgaat gccggatgtg
     1861 gacttggggc ccggagggcc ttgatttgat tgcaggaggt ccgcgccgta tgtaacacgc
     1921 gccgatgctg ggtaagccgc cccctcatgt gatcatcgac gggtttctcg gccccgagct
     1981 tcatggtgcg ctgctcgatt gggcgctcac cggcgaacgg cgcttttcct gttcccaggt
     2041 ccagggcgag gatggcctcg tgcacaatgc cggtgtgcgc cagtcgtggc actgcgatga
     2101 tgggcttggc ccgctcaagg cgcagttccg cgcctcgatc gatacagcgc gcgagaccat
     2161 ccggcagcag ctcggcatac cccccttcgc cgtggtccgc accgagctgg aattggccgc
     2221 ccaccgcgac ggctcgttct acaagcccca tgtcgacacc catgtagggg ccggccgggc
     2281 aagcaccgac ggcgaccgga tgctgaccct ggtttactat ttgcaccgcc agccccgcgg
     2341 cttttccggc ggagaaattg cgctctatcc attcaacctt gccgctccga ccctgatcca
     2401 gcccgcggac aaccgcctga tcgccttccc cgcccatgcc ctgcacgagg ttcgcccggt
     2461 ggcggtcccc ggcgacgcct gggaagatgc gcggtttgcc atcaactgct ggtataaccg
     2521 cgagcgaccg gtctgaggac caaattcggg atgctggacc gcgcgctcag gccagcttcc
     2581 actcccagcc aagcggatcg ccatccatca cctcgacgcc gcgggctgcc agttcttccc
     2641 gcaacgcatc tgagcgggcg aaatcttttg ccgcccgggc ttccttgcgg gcggtgaggc
     2701 tttgttcgat gtcggcctcg gcgatcgtcg cggccttggg acggaggcgg aggtcggcgc
     2761 gcccaagttc gagcaggccc atccccagca ctgcgtccat cgcagcgatg gccgccagct
     2821 tctcgcccgc atccaccttc ttcagggcaa ttgcctcgtc gagcacggtc agagcaacgc
     2881 tagtattgag gtcatcggaa atcgccgcat cgaaacgctc gagcagggct gcgaacttca
     2941 ggcccggtgc gggtgccggc tcgccctggg cgcgaaggtt ggctacctgc attaccatcc
     3001 gcttcagccg cgtcagcgcc gcgccgagcc cttcccagga gaactccagc tcgctgcggt
     3061 agtgcgcctg caggcacatc aggcggtagg cgagcgggtg gtagcccttg tcgatcagca
     3121 gctgcagccg caggaattcg cccgacgact tgctcatctt gccgctgcgt tcgacgagaa
     3181 agttgttgtg catccatacc cgcgcgccgg aatttgccgg ctggtcgaga ccattcgtgc
     3241 agcagtgtgc ctgattctgg gcgatctcgt tggggtggtg gatctcccgg tggtcgatcc
     3301 ccccggtgtg gatgtcgaac ggaaagccga gcacctcgcc gctcatcacc gagcactcga
     3361 gatgccagcc gggcgcaccg cggccccacg gcgaatccca ttccatctgg cgcttttcgc
     3421 ccggcggggt cttgcgccag atggcgaagt cggccccgtt gcgcttgcct tcgacggcct
     3481 cgatccggcc ctccccctca tcggtctgcg cgcgggcgag gcgaccgtag tcggccacgg
     3541 tggagacgtc gaagtagagg ccgctgtcga gctcgtagca gtgcttttca gcgatgcccc
     3601 tggcgaattc gatcatctgc gggacatagt cggtcgcgac cgtccacttc gccggctggc
     3661 gtatattgag cgcccgaatg tcggcccagt aggcctgctt gtagtgttcg gcgatgtccc
     3721 agatcgagcg cgcctcggcg cgggccatct tctccatctt gtcctcgccg gcatcggcat
     3781 cgtcggtgag gtggccgacg tcggtgatgt tgatgacgtg ggtcagcctg tagcccttga
     3841 agctcagcgt acggcccagc acgtcggcaa agacatagga acgcatgttg ccgatgtggg
     3901 gatagttgta gaccgtcggg ccgcaggtat agacgcgcgc ctctccagcg tggaccggct
     3961 ggaacggctc ggccgcgcgg gtgaggctgt tgtagaggac gagcggggct tgggcttcgg
     4021 tcatggcgct ggcgccatgc ggcggtttgg acggccggtc aacctgccgg gacgcgcctc
     4081 aggccggcac cgcctcgaac ccgaggaacc gcacctgctc ctccagctct gcgcgcgggc
     4141 gggtgaaatt gttcactgcc gccgtctcgt cgcggtagcc cagggccagg ccgcagaaca
     4201 ggagcgtatc gtcagacagg ccgaggtgcg ctttgatcgt gcggccatag aggcccatgt
     4261 attcctgcgg gcaggtatcc aggccggctt cgcgggccaa cagcatgacg gtttgcagcc
     4321 acatcccgat atcggaccac tgcgcttcgc cgaccagctt ggggaagtgg ctgaggatca
     4381 gcacgggggc gccaaaggag atcttgttct ggcgagtgaa ctcggcccgt ccggcggcat
     4441 cgctgcgatc gacgcccgtc gcgccgtaca tcgccgcgcc gagcgattgc acgcgcgcca
     4501 gatgccgggg cgaggcattg ggcttgtcgt agcggtattc gagcggatca tccacttcgg
     4561 ccggcagcag aaccgcctgt agtgccttca gtggctcccc cgtcagcacc gtggcttccc
     4621 aggtctggaa gttgcagccc gagg
//
LOCUS       DATNBV010000074         4764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_359413,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000074 DATNBV010000000
VERSION     DATNBV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359413"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VN627_01745"
     CDS             <1..558
                     /locus_tag="VN627_01745"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXD02008.1"
                     /translation="AEARVIPDALVLSAPMLGFVRHGLPPVLSRWVAKLMCAIGDPRR
                     PAWGGSGREKPGQPPADRIALLTHDAGRYADEAWWRDRRPGLGMGPPSWGWLRAALAS
                     MQRLDRRETLARVTVPTFIAATTADRLVDYGAIARAARWIPGAQLLRLGKEARHEVLR
                     EVDPVRDRVIAAIDEFLDRTAPAAK"
     gene            566..1669
                     /locus_tag="VN627_01750"
     CDS             566..1669
                     /locus_tag="VN627_01750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXD02009.1"
                     /translation="MTDSAYPARFDFAVIGAGMAGASLAAQLAPTGARVLLLEAELVA
                     GYHATGRSAAFWTESYGGPGVQPLTTASGPALRGGGYLAPRGALTIARAGQEDELARF
                     AERYEALGVRVERLDREAIAARVPGLRQGWVAGALEPDCCDIDVARLHQDYLAAASRA
                     GAVLWTGARLAAAEESPSGWALALADGRSVRADVLVNAAGAWADRVAAMAGQPGIGLQ
                     PYRRTVAQLRTSPAPSNGLPLVLDLAEKFYFKPQSGRLWLSPHDETPSQPCDAAPEDI
                     DVATAIARLEEVVVWQVERVEHRWAGLRSFAPDRLPVYGFAGASPRFFWFAGQGGFGI
                     QTAPAAAALGASLLVPGRASPVDPGRYSPCRFG"
     gene            1747..1929
                     /locus_tag="VN627_01755"
     CDS             1747..1929
                     /locus_tag="VN627_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HXD02010.1"
                     /translation="MAHRFEIRQNKAGEYVAYFCHNAETIFWTEGYKSKASARNAIDS
                     VLKNGPGAEVVDTTAG"
     gene            2009..2260
                     /locus_tag="VN627_01760"
     CDS             2009..2260
                     /locus_tag="VN627_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02011.1"
                     /translation="MSLYDQELEQARQVLARSGADPEAFGFAMDYLPPDDTDGGGMFT
                     QEYEVVVTAPSGRSQRYVGAIGRDWVAVFARHLGEGAFR"
     gene            complement(2263..2808)
                     /locus_tag="VN627_01765"
     CDS             complement(2263..2808)
                     /locus_tag="VN627_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02012.1"
                     /translation="MNDRTESADMDSARIALGSLAATGAGILLGLAANPTMRSLPGTD
                     WRERYRSAAAAPEFQPQAGVSYFGDGSTAWVYGGVPQRISDRTLPLQPPAAIPEYDYR
                     PEPYDATPVESPADADAQGAAERAAAQAAVAAALVRSAPTTSQEHAGEERGAEIDAGP
                     ATASAAATTPPVATLAEGGPT"
     gene            complement(2861..4270)
                     /gene="lpdA"
                     /locus_tag="VN627_01770"
     CDS             complement(2861..4270)
                     /gene="lpdA"
                     /locus_tag="VN627_01770"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXD02013.1"
                     /translation="MPDQTFDYDVLVIGAGPGGYVAAIRAAQLGLKTACVESRETLGG
                     TCLNVGCIPSKAMLHASEYFDAAANGAMAKLGVKVTPELDLGAMHGQRLDAVKQLTGG
                     IEFLFKKNKVDWLKGRAAFVDAHTVALRQAEGERTVSAKNVVIATGSSVAPLPGVAID
                     QQVVVDSTGALELPAVPKRMVVIGGGVIGLELGSVWRRLGAEVTVVEFLDQLLPGMDM
                     DVRKEAAKIFAKQGMTLKLGTKVTGVAVKGKAAKVTVEPAAGGAAEVLDADVVLVAIG
                     RRPNTDGLALDRIGLELNQRGQIKTDHDFATRVPGVWAIGDVIPGPMLAHKAEDEGIA
                     VAENIAGLTGIVNHEVIPGVVYTQPEIAGVGLTEEAAKERGEIKVGKFPMLANSRAKT
                     NHEPDGFVKVIADAKTDRVLGVWCIASVAGTMIAQAAQAMEFGATSEDIAYTCHAHPT
                     HSEAIKEAAMAVQGKPIHM"
     gene            complement(4291..>4764)
                     /locus_tag="VN627_01775"
     CDS             complement(4291..>4764)
                     /locus_tag="VN627_01775"
                     /inference="COORDINATES: protein motif:HMM:NF020544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HXD02014.1"
                     /translation="RANPDSIGQFIVGTDVDEELLDAPADVHNVAIPFDLDDQWQAIH
                     FLLTGSAEATADPLSIIVGKFEKIGRDQGFGPAWIIPAERILAADAALSDLDDATLRE
                     RYDPKAMVRHNVYGAKLLAEEGPGGVDFVMDDVERLRSFLAEGAARGLAALAMIN"
BASE COUNT          644 a         1668 c         1649 g          803 t
ORIGIN      
        1 gccgaggcgc gggtgattcc cgacgccctg gtgctttccg ccccgatgct cggcttcgtg
       61 cgccatggct tgccgccggt gttgtcgcgc tgggtggcca agctcatgtg cgccataggc
      121 gacccgcgcc ggccggcctg gggcgggagt gggcgcgaaa agccgggcca gccgcccgcc
      181 gaccggatcg ccctcctcac ccatgatgcc gggcggtatg ccgacgaggc ctggtggcgc
      241 gaccgtcggc ccggcctcgg catgggcccg ccgagctggg gctggctgcg cgcggccctg
      301 gcctcgatgc agcggctcga ccggcgcgag accctggccc gcgtcaccgt ccccaccttc
      361 atcgcggcga ccacggcgga ccggctggtc gactatggcg caatcgcccg cgccgcccgc
      421 tggattccgg gcgcgcagtt gctgcgcctg ggcaaggagg cgcgccacga agtcctgcgc
      481 gaggtcgatc cggtgcgtga ccgcgtcatc gccgcaatcg acgaattcct tgaccggacg
      541 gcgcccgctg ccaagtgacg ctcccgtgac cgattccgct tatcccgcaa ggttcgattt
      601 cgccgtgatc ggcgccggca tggccggggc cagcctcgcc gcgcagcttg cccccaccgg
      661 cgcgcgcgtg ctcctgctcg aggccgagct ggtcgccggc tatcatgcca ccggccgctc
      721 cgctgccttc tggaccgaga gctacggtgg ccccggcgtc cagccgctga cgaccgcgtc
      781 cgggccggcc ctgcggggtg gtggctatct tgctccacgc ggcgcgctga ccatcgcccg
      841 cgccggccag gaggacgaac tggcgcggtt tgcggaacgc tacgaggcac tcggggtgag
      901 ggtggagcgg cttgaccgcg aggccattgc cgcgcgtgtc ccgggcctgc gccagggctg
      961 ggttgccggg gcgctggagc ccgattgctg cgacatcgac gttgcccgcc tgcatcagga
     1021 ctatcttgct gcggcgagcc gtgccggcgc ggtcctgtgg acgggtgcgc ggctggccgc
     1081 cgcagaggaa agcccctccg gctgggccct ggcgctagcc gatggccgct ccgtaagggc
     1141 agacgttctg gtcaatgccg cgggggcctg ggccgaccgc gtcgccgcca tggccgggca
     1201 gcccggcatc ggcctccagc cctatcgccg cacggtcgcg caactgcgca ccagccccgc
     1261 gccctccaac gggctgccgc tggtgctcga ccttgccgaa aagttctact tcaagccgca
     1321 gtccggtcgc ctctggctca gcccgcacga tgaaaccccc agccagccct gcgacgccgc
     1381 gcccgaggac atcgacgttg ccaccgccat cgcgcggctg gaggaggtgg tcgtgtggca
     1441 ggtcgagcgg gtcgagcatc gctgggccgg cctgcgctcc ttcgcgcccg accgcttgcc
     1501 ggtctatggc ttcgccggcg cgagtccgcg cttcttctgg tttgccgggc agggcggttt
     1561 cggcatccag acggcgccgg ctgccgcggc cctgggggcc tccctgctgg tgccggggcg
     1621 cgccagtccg gtcgatcccg ggcggtattc gccatgccgc ttcggctgat ccctggatgc
     1681 aatcggttga attcgccagt ggcagtgcta gcatcgaggc ggagtcgcga caactggagt
     1741 accccaatgg cgcaccgttt cgagatccgt caaaacaagg ccggcgaata tgtggcctac
     1801 ttctgccaca atgcggagac catcttctgg accgagggct acaagtcgaa ggccagcgcc
     1861 cggaacgcga tcgattcggt cctgaagaac ggccccgggg ccgaggtcgt cgatacgacc
     1921 gcaggctgac cgccggctcg cgcagcgttg cgtcgctcgc ccctcgggca tagcctgcga
     1981 cggcagcgga tggcagcgag gtactcagat gagcctgtac gatcaggagc ttgaacaggc
     2041 gaggcaggta ctggcccggt caggcgcgga tccggaagcg ttcggcttcg ccatggacta
     2101 cctgccgccc gacgacacgg acggcggcgg gatgttcacc caggaatacg aggtggtcgt
     2161 aacggcgccc tcgggccgct cgcagcgtta cgtcggcgcg atcgggcggg actgggtggc
     2221 cgtcttcgca cgccatctgg gcgagggcgc tttccgctga tgtcaggtcg ggccgccctc
     2281 ggccagggtg gccactggcg gggttgtcgc agctgctgac gcggtggcgg gtccagcatc
     2341 gatttccgcg ccgcgttcct cgccggcatg ctcctggctg gtggtcggcg ccgaccgcac
     2401 cagggcggcg gcgacggccg cttgcgctgc cgcccgctcg gcagcgccct gggcgtctgc
     2461 atctgccggg gattctacag gtgtcgcatc gtagggctcg ggacggtagt cgtattcagg
     2521 tatggccgcg ggcggctgca gagggagagt cctgtcgctg atgcgctgcg ggaccccgcc
     2581 atagacccag gcagtcgagc catccccgaa gtaggaaacc cctgcctgtg gctggaattc
     2641 cggcgcggcg gcagccgagc ggtagcgctc acgccagtcg gtgccgggca ggctgcgcat
     2701 ggtcggattg gcggcgagcc ccagcaggat gcccgcaccc gtcgcggcga gggagccgag
     2761 ggcaatgcgg gcactatcca tgtcggcgct ctccgtgcgg tcgttcaacc gggaaaacgc
     2821 gtcattactg gccccgttcc ggctgcggcc gcgcttgcgc ctacatgtgg atcggcttgc
     2881 cctgcacggc catggccgct tccttgatcg cttccgaatg ggtcggatgg gcgtggcagg
     2941 tataggcgat gtcttcgctg gtcgcgccga attccatggc ttgcgccgcc tgggcgatca
     3001 tcgtgccggc aacgctggcg atgcaccaca cgccgagtac gcggtcggtt ttcgcgtcgg
     3061 cgatgacctt cacgaagccg tccggctcgt gattggtctt ggcccggctg ttcgccagca
     3121 tcgggaattt gccgaccttg atctcgcccc tttccttcgc ggcttcctcg gtcaggccga
     3181 ccccggcaat ctccggctgg gtatagacca cgcccgggat cacctcgtgg ttcacgatac
     3241 cggtaaggcc cgcaatgttc tcggccacag cgatgccctc gtcctcggcc ttgtgcgcca
     3301 gcatcgggcc ggggatcacg tcgccgatag cccagacgcc cggcaccctg gtggcgaagt
     3361 cgtgatctgt cttgatctgc ccgcgctggt tcagttcaag tccgatccgg tcgagcgcca
     3421 ggccgtccgt gttcgggcgg cggccgatgg cgaccagcac cacgtcggcg tccagcactt
     3481 ccgccgcgcc gcccgcggcg ggttctaccg tgaccttggc ggctttgccc ttgaccgcca
     3541 cgcccgtcac cttggtgccg agcttcaggg tcatgccctg cttggcgaag atcttggcgg
     3601 cttccttgcg cacgtccatg tccatgccgg ggagcagctg gtcgaggaat tcgaccacgg
     3661 ttacctcggc gccgagccgc cgccagaccg aacccagctc cagcccgatc acgccgccgc
     3721 cgatgacaac catccgcttc ggcaccgccg gcagttccag cgcgcccgtg ctgtcgacca
     3781 cgacctgctg gtcgatggca acgcccggca gcggggcgac ggacgagccg gtggcgatga
     3841 ccacgttctt cgcacttacg gtccgctcac cctcagcctg tcgaagggcc accgtgtggg
     3901 cgtcgacgaa ggcggcgcgg cccttgagcc agtccacctt gtttttcttg aacaggaact
     3961 cgatgccccc ggtcagttgc ttcaccgcat ccagccgctg gccgtgcatg gccccgaggt
     4021 ccagctcggg cgtgaccttc actccgagct tggccatggc cccgttcgcc gccgcgtcga
     4081 aatattccga ggcgtggagc atggccttcg acgggatgca gcccacgttg aggcaggttc
     4141 cgcccagcgt ctcgcggctc tccacgcagg ccgtcttcag cccgagctgc gcggcgcgga
     4201 tcgccgcgac atagccgcct ggcccggcgc cgatgacgag gacgtcgtag tcgaaggtct
     4261 ggtcgggcat ggcgtcagga gtccgttatg tcagttgatc atggcgagcg cggcgaggcc
     4321 gcgagccgcg ccttcggcga ggaacgagcg cagccgttcc acgtcgtcca tcacgaaatc
     4381 caccccgccc gggccttcct cggccagcag cttggcgccg tagacgttgt gccgcaccat
     4441 cgccttggga tcgtagcgct cgcgcagggt ggcgtcgtcg aggtccgaca gggccgcatc
     4501 ggcggcgagg atccgctcgg ccggtatgat ccaggccggg ccgaagccct ggtcgcggcc
     4561 gatcttctcg aacttgccga cgatgatcga caacggatcg gcggtcgcct cggccgagcc
     4621 ggtcagcagg aaatggatcg cctgccactg gtcgtcgaga tcgaacggga tcgccacgtt
     4681 gtgcacgtcg gcgggcgcgt cgagcagttc ctcatccacg tcggtgccga cgatgaactg
     4741 cccgatcgaa tcggggtttg cccg
//
LOCUS       DATNBV010000075         6059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1638736,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000075 DATNBV010000000
VERSION     DATNBV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638736"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..93
                     /locus_tag="VN627_01780"
     CDS             <1..93
                     /locus_tag="VN627_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXD02015.1"
                     /translation="HCKTCDIKDPTQNIEWVTPEGGGGPNYPNM"
     gene            245..1048
                     /locus_tag="VN627_01785"
     CDS             245..1048
                     /locus_tag="VN627_01785"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HXD02016.1"
                     /translation="MSLHETAYAKLNLALHVRARRADGYHELETLFAFVDAGDVLTAR
                     PSALDELHILGEFAGALADPFDNIVMQALTALPRAEGLAITLEKNLPVAAGLGGGSAD
                     AGAVFRLVERSHGWPDDSATRAARLGADFPACVRSETCLGRGTGTDLEPGPTDLAGTP
                     VLLVNPRLPLATGPVFRAWDGLDRGPLPTGTARQIALAGRNDLEMPAISLCPQIAEVH
                     TALRSTGAWLTRMSGSGATCFGLYADEAQRDAAAATMPREWWQMAGKLR"
     gene            1045..1767
                     /locus_tag="VN627_01790"
     CDS             1045..1767
                     /locus_tag="VN627_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HXD02017.1"
                     /translation="MTEVFRLIGQPRFGGLLVVSDHASNHVPEDIALGIDPALLTQHM
                     ALDIGVAEVGALMAERRGMAAFQCAVSRLVCDVNRNEDAPGAVPEFSDGHAIPGNHLT
                     QSARSARIARFHAPYHAALDRVLGEAPPQLILSLHSFTPALATCDKPRPWQVGVLYNR
                     DDRAARRAIPLLEAEGLCVGDQQPYSGVELNYTMNRHAEAEGRAYLGIEIRQDEIDHP
                     AGQQRWAERLGRIANAVALQMS"
     gene            1972..3822
                     /gene="ilvD"
                     /locus_tag="VN627_01795"
     CDS             1972..3822
                     /gene="ilvD"
                     /locus_tag="VN627_01795"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXD02018.1"
                     /translation="MPAYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFT
                     QFVPGHVHLKDLGQMVAREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAALRINIPVVFVSGGPMEAGKVVLKGKEV
                     ALDLVDAMVAAADDDNYTDEEVQSIERSACPTCGSCSGMFTANSMNCLTEALGLSLPG
                     NGSTLATHADRQHLFLEAGRLVVDLCKRYYEQDDASVLPRAIASFKAFENAISLDIAM
                     GGSTNTVLHLLAAAHEAGVDFTMADIDRLSRKVPCLSKVAPAKNDVHMEDVHRAGGIM
                     AILGELDRAGLLHTELPTVHSRTMRDALNQWDIARTNSPKVQEFFKAAPGGVPTQVAF
                     SQSRRWDSLDLDRENGVIRSKEHAFSQDGGLAVLYGNIARDGCIVKTAGVDESILRFT
                     GPAKVYESQDAAVTAILTGQVVAGDVVVIRYEGPKGGPGMQEMLYPTSYLKSKGLGKA
                     CALLTDGRFSGGTSGLSIGHASPEAAEGGEIGLVETGDTIEIDIPGRTINLAVSDEVL
                     SHRRAAQDEKGWHPAKERPRKVSDALKAYAAMTTSAARGAVRDVSQLKRS"
     gene            3825..4190
                     /locus_tag="VN627_01800"
     CDS             3825..4190
                     /locus_tag="VN627_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02019.1"
                     /translation="MRRGLAIAVLSLLAAACSRGGPGGGAAADGAEKIACALGPDAKF
                     APVCALERAERGGEKLFVVHHPDGGFRSFTVLANGAGLAAADGAEPASQAIAGDQLEV
                     DVGEDRYRFPFKAAPHGAN"
     gene            4177..5571
                     /locus_tag="VN627_01805"
     CDS             4177..5571
                     /locus_tag="VN627_01805"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HXD02020.1"
                     /translation="MEPIEQVLTVAQMRAAEEDLIRAGSSVDALMQIAGRGAAEWVWR
                     MAGHHPVTVLCGPGNNGGDGYVIAEALRERGGKVRVVAAVEPKTDAARNARALYHGEV
                     VGPGAEVHGDVLVDCLLGSGLVRPLSAELFALLTRLAAHHHRTIAVDLPSGVESDSGV
                     ALNPGLPVNQLTIALGAWKQAHFLMPAAAAMGALRLVGIGCAAVPGAPGVIARPPIGT
                     PDAETHKYRRGLLAVVGGAMSGAAELACAAAMGAGAGYVRLCSDGRAVQPRAAALVVD
                     ARPLTEALADDRIAATLVGPGLGRDAAARGRLEAALATGHRLVLDADALVLMRRGDNA
                     PAGVATPHDGELAALERAFGLDGIGTKLDRARALAQATGLVILAKGPDTVIAAPDGRA
                     AFAHRASVWLSTAGTGDVLAGIVASRLATGLAPFEAASEAVWLHGEAARLAGKAFSAD
                     DLAAHVQPAYAACL"
     gene            5568..>6059
                     /locus_tag="VN627_01810"
     CDS             5568..>6059
                     /locus_tag="VN627_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HXD02021.1"
                     /translation="MSTAEEILRIAAKGDGVTASGRFAWGAAPGDLLNEDGTLEWGPH
                     HVAPICRHFGQCGGCQLQQLDEQSLVQFIEARVANASTSQGLGAEHFAPPHLSPAGTR
                     RRASLRAESSQGRVVIGYREARSHRVVELAECPVLVPELVAILGPLRKLLIRLGQGAA
                     KKPK"
BASE COUNT          925 a         2251 c         1942 g          941 t
ORIGIN      
        1 cactgcaaga cctgcgacat caaggacccc acccagaaca ttgaatgggt cacgcccgaa
       61 ggcggcggcg ggccgaacta tccgaatatg taaccagcga tctctcccca ttggggagag
      121 atacgcaggt ttggcagcgt gctgcctagc cgaagttgag agggggtcgg cactggtctg
      181 ccaggtccgc aacccctttc ccaaccctct ccccttcagg gaagagggct actatggttc
      241 gcccatgagc ctccacgaaa ccgcctacgc aaagctgaac ctcgcgctcc acgtccgcgc
      301 gcggcgggct gacggctacc atgagctcga aaccctgttc gccttcgtcg acgccggtga
      361 cgtgctgacc gcgcgcccgt cggcgctcga cgaactgcac atccttggcg aattcgccgg
      421 cgcgctcgcc gatcccttcg acaacatcgt catgcaggcg ctgacggcgc tcccccgcgc
      481 cgaagggctg gcgatcactc tcgaaaagaa cctccccgta gcggccggtc tcggcggcgg
      541 ttcggccgat gccggcgcgg tcttccggct ggtggaacgc agccacggct ggcctgatga
      601 ctcggcgaca cgcgccgctc gtctcggcgc cgactttccc gcctgcgtgc gcagcgagac
      661 ttgcctcggt cgcggcacgg gcaccgacct tgaacccggc cccaccgacc ttgccggcac
      721 ccccgtcctc ctcgtcaacc cgcgcctgcc gctcgccacc ggcccggtgt tccgggcctg
      781 ggacgggttg gaccgaggcc cgctgcccac gggaacggcg cgtcagatcg cactcgctgg
      841 ccgcaacgac ctcgaaatgc ccgctatttc gctctgtccg cagatcgccg aggtccatac
      901 ggcgctgcgc agcaccggtg cgtggcttac gcgcatgtcc ggctcgggcg cgacctgttt
      961 cgggctctac gccgacgaag cccagcgcga tgcggccgcc gccaccatgc cgcgcgaatg
     1021 gtggcaaatg gcaggcaagc tcagatgacc gaagtcttcc gcctcatcgg ccagccccgc
     1081 ttcggcggac tgctcgtcgt ctcggaccac gcctccaacc atgtgcccga agatatcgcc
     1141 ctcggcatcg atccggccct gctgactcag cacatggcgc tggatatcgg cgtggcggaa
     1201 gttggcgccc tcatggccga gcgccgcggc atggccgcgt tccagtgtgc cgtcagccgc
     1261 ctcgtctgcg acgtcaaccg caacgaggat gcccccggcg ccgtcccgga attcagcgac
     1321 gggcacgcga tccccggcaa ccacctcacg caatccgctc gctccgcccg cattgcccgc
     1381 ttccacgcgc cctatcacgc cgcgttggac cgcgtgctcg gcgaggcccc gccgcagctg
     1441 atcctctcgc tccactcctt caccccggcg ctcgccacct gcgacaagcc tcgtccttgg
     1501 caggtgggcg tgctctacaa ccgggacgac cgcgccgccc gccgcgccat tcccctgctc
     1561 gaggcggaag gcctgtgcgt cggcgatcag cagccctatt cgggcgtgga gctcaattac
     1621 accatgaacc gccacgccga agccgagggc cgcgcctacc tcggcatcga gatccgccag
     1681 gacgagatcg accaccctgc cggccagcag cgctgggccg aacggctggg ccggatcgcc
     1741 aatgccgtgg cgctgcagat gagctgacgt cgtcccggac ttgatccggg accggtgtca
     1801 gatgcgaagc cgtgacctcg cctccaggca cacctgcagc tccgctaccc accccggtcc
     1861 cggatcaggt ccgggacgac ccgttccgcc catttccgtt cgacctcgcc gccttgctgt
     1921 tctacaggcc ggcctccata tccaagcgac agaccttacc ggagccgacc catgcccgcc
     1981 taccgttccc gcaccaccac ccacggtcgt aacatggcgg gagcgcgcgg cctgtggcgc
     2041 gccacgggga tgaaggacag cgatttcggc aagccgatca ttgccgtggt gaactcgttt
     2101 acccagttcg tccccggcca cgtccacctc aaggacctcg gccagatggt cgcccgggag
     2161 atcgaggcag cgggcggagt tgccaaggaa ttcaatacca tcgcggtgga tgatggcatc
     2221 gccatgggcc atgacggcat gctctattcg ctgccgagcc gtgatctgat tgccgactcc
     2281 gtcgaataca tggtcaacgc ccactgcgcc gatgccatgg tctgcatctc caactgcgac
     2341 aagatcaccc ctggcatgct gatggcggcc ctgcggatca acatcccggt ggtcttcgtc
     2401 tcgggcgggc cgatggaggc cggcaaggtc gtcctgaagg gtaaggaagt cgcgctcgat
     2461 ctggtcgacg ccatggtcgc cgcggccgat gacgacaatt acaccgacga ggaagtgcag
     2521 agcatcgagc gctcggcttg ccccacctgc ggctcctgct cgggtatgtt caccgccaat
     2581 tcgatgaact gtctgaccga ggcgctcggc ctctcgctcc ccggcaacgg ctccacgctg
     2641 gctacccatg ccgaccgcca gcacctgttc ctcgaagcgg gccgcctcgt ggttgacctg
     2701 tgcaagcgct attacgagca ggacgacgcc tcggttctcc cgcgcgccat cgccagcttc
     2761 aaggccttcg agaacgcgat cagcctcgat atcgccatgg gcgggtcgac caacaccgtg
     2821 ctccacctgc tggccgccgc gcacgaggcc ggggtggact tcaccatggc cgatatcgac
     2881 cggctttccc gcaaggtgcc ctgcctctcc aaggtcgccc cggccaagaa cgacgtccac
     2941 atggaagacg tccaccgcgc cggcgggatc atggcgatcc tgggcgagct ggaccgcgcc
     3001 ggcctgctcc acaccgagct gcccaccgtc cacagccgca ccatgcgcga tgcgctcaac
     3061 cagtgggaca tcgcccgcac caattccccc aaggtgcagg aattcttcaa ggccgcgccc
     3121 ggcggcgtgc ccacccaggt tgccttcagc cagtcacgcc ggtgggacag cctcgatctc
     3181 gatcgcgaga acggcgtgat ccgcagcaag gaacatgcct tcagccaaga cggcggcctt
     3241 gccgtgctct atggcaatat cgcccgcgac ggctgcatcg tgaagacggc gggggtggac
     3301 gaatccatcc tcaggttcac cggcccggcc aaggtctatg aaagccagga cgccgcggtc
     3361 accgcgatcc tcaccgggca ggtggtcgcc ggcgatgtcg tggtgatccg ctacgaaggc
     3421 cccaaggggg gcccgggcat gcaggaaatg ctctacccca cgagctacct caagtcgaag
     3481 ggccttggaa aggcctgcgc gctcctcacc gacgggcgct tctccggcgg cacctcgggc
     3541 ctgtccatcg gccacgcctc gccggaagcg gccgaaggcg gtgagatcgg cttggtcgag
     3601 actggcgaca ccatcgagat cgatatcccc ggccgcacga tcaaccttgc cgtgagcgac
     3661 gaggtcctct cccaccgccg cgccgcgcag gacgagaagg gctggcaccc ggccaaggag
     3721 cgtccgcgca aggtctccga cgcgctgaag gcctatgccg cgatgaccac cagcgccgcc
     3781 cgcggcgccg tgcgcgacgt ctcgcaactc aagcgctcct gaccatgcgc cgggggctcg
     3841 ccatcgctgt cctgagcctg ctcgctgccg cctgctcgcg cggcgggccg gggggcggcg
     3901 ctgcggccga tggcgcggaa aagatcgcct gcgcgcttgg cccggatgcg aaattcgctc
     3961 ccgtctgcgc actcgagcgg gctgagcgcg ggggcgagaa gctgttcgtc gtccaccacc
     4021 cggacggcgg cttccgcagc ttcaccgtgc tcgccaatgg cgcgggcctc gccgccgccg
     4081 atggagccga accggccagc caggccattg ccggcgacca gctggaagtg gacgtgggcg
     4141 aggaccgcta ccgcttcccc ttcaaggccg cgcctcatgg agccaattga gcaggtcctg
     4201 accgtggcgc agatgcgcgc cgccgaggag gacctgatcc gcgccgggtc cagcgtcgat
     4261 gccctgatgc agattgccgg gcgcggcgcg gctgaatggg tctggcgcat ggcagggcac
     4321 catccggtca ctgtgctgtg cggtccgggc aacaacggcg gcgacggcta tgtgatcgcc
     4381 gaggcgctgc gcgaacgcgg cggcaaggtc cgcgtcgtcg ctgcggtcga gccgaagacc
     4441 gacgccgccc gcaacgcccg tgcgctgtac catggcgagg tcgttggtcc cggggccgag
     4501 gtccatggcg acgtgctggt cgattgcctg ctgggcagcg gcctcgtccg cccgctgtcg
     4561 gccgagctgt tcgccctgct cacccgtctt gccgcccatc atcaccggac catcgccgtc
     4621 gatttgccga gcggggtcga gagcgacagc ggcgtggcgc tcaaccccgg cctgccggtc
     4681 aaccagctca ccatcgcgct tggcgcatgg aagcaggcgc atttcctgat gccggccgcc
     4741 gccgccatgg gcgcgctgcg gctggtcggg atcggctgcg cggccgtccc gggagcgcca
     4801 ggcgtgatcg cccgcccgcc gatcggcaca cctgatgccg aaacccacaa gtaccgccgc
     4861 ggcctgctgg ccgtggtcgg cggcgccatg tcgggcgcgg cggaactcgc ctgcgccgcg
     4921 gccatggggg cgggggccgg ctatgtccgc ctgtgcagcg atggccgcgc ggttcagccc
     4981 cgcgccgccg cgctggtggt tgatgcccgt ccgctgacgg aagcgctggc cgacgaccgc
     5041 atcgccgcca ccctcgtcgg ccccggcctt ggccgcgatg ccgctgcacg gggtcgcctt
     5101 gaggcagcgc ttgccaccgg gcatcgcctc gtcctcgatg ccgatgcgct ggttctgatg
     5161 cgtcggggcg acaatgcgcc agccggcgtc gccaccccgc acgatggcga actcgctgcc
     5221 ctggaacgcg ccttcgggct cgatggcatc ggcaccaagc tcgaccgcgc ccgggcgctt
     5281 gcccaggcca cgggcctggt catcctcgcc aagggccccg acaccgtcat cgccgcgccg
     5341 gacggtcgcg ccgccttcgc tcaccgggcc tcggtctggc tttccacggc aggaaccggg
     5401 gacgttctcg ccggcatcgt tgcctcgcgc cttgccaccg gcctcgcgcc attcgaagct
     5461 gcctccgagg ccgtctggct ccacggcgag gccgcgcgtc ttgcgggcaa ggccttttcc
     5521 gccgacgatc ttgccgctca cgtccagccc gcctacgccg cctgcctgtg agcaccgctg
     5581 aagaaatcct gcgcatcgcc gccaagggcg atggcgtcac cgcctcgggc cgcttcgcct
     5641 ggggcgcggc gccgggtgac ctcttgaacg aggacggcac gcttgaatgg ggcccgcacc
     5701 acgtggcgcc tatctgccgc cattttggcc agtgcggcgg ctgccagctc cagcagctcg
     5761 acgagcagag cctggtccaa tttatcgagg ctcgcgtcgc caatgcctcg acctcccagg
     5821 ggttgggggc cgagcacttt gctccgccac acctctcgcc agccggcacg cgtcgccgcg
     5881 cctcgctgcg cgcggaaagt tcgcagggcc gcgtggtcat cggctaccgc gaagcccgct
     5941 cccaccgcgt ggtcgagctc gccgaatgcc cggtgctggt gcccgaactg gtcgccatcc
     6001 tcggcccctt gcgcaaactg ctgatccggc tggggcaggg cgcggccaag aagcccaag
//
LOCUS       DATNBV010000076         3962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_56721,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000076 DATNBV010000000
VERSION     DATNBV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56721"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..933)
                     /locus_tag="VN627_01815"
     CDS             complement(163..933)
                     /locus_tag="VN627_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXD02022.1"
                     /translation="MTEIAYHQLPAGPRIAYRFTDGTGPVLVFLPGYMSDMAGSKAEA
                     LFGWARATGRACLLLDYSGCGQSGGSFAEGTLSRWRDEVLALVAALCAGRRLVLVGSS
                     MGGWLMLLVALALNDSGPGRVAGLVGIAAAPDFTEWGFDEAQQALLATGEVLLEDNPY
                     GPEPTPTHPGFWRDGQGNVLLAGEIALDCPARLLQGQADADVPWDVALRLVRALRSAD
                     VQAILIKDGDHRLSREADIALLLRTVQALLGELAGQES"
     gene            1017..3008
                     /gene="thrS"
                     /locus_tag="VN627_01820"
     CDS             1017..3008
                     /gene="thrS"
                     /locus_tag="VN627_01820"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXD02023.1"
                     /translation="MSEMITISLPDGSSREMPQGSTPLDVAAAIGPGLAKAALAARVD
                     GELVDLTRPFTGDAQLALITSRDEAEALDLARHDYAHVLAEAVQALFPGTQITFGPST
                     DDGFYYDFAPKDRPFTDEDLPAIEAEMRKIIAANKPLRREVWSREQLISRWQQQGESF
                     KAEWAAELPGDEDLTVYWSGDDWLDMCRGPHLPSTGKLDPAAFKLTRVSGAYWRGDQK
                     NAMLSRIYGTGWLSKKQLDAHLTRLEEAAKRDHRKLGAEMDLFHLQQEAHGSVFWHPN
                     GYKIWRELEAYMRRAIDAAGYREVKTPQVMDARQWEQSGHWGKYRENMFVIPDEVPNV
                     DDEGPVISGEADWMALKPMNCPAHVLIFRQGIKSYRDLPLRIYENGCCHRNEPHGALH
                     GLMRVRQFTQDDAHIFCREDQIVAEVRAFCELADRIYQHFGFTYSIKLALRPEKRFGS
                     DADWDKAEGELRNAVAEAGLATPEYGWEELPGEGAFYAPKLEWHLTDAIGRTWQVGTI
                     QSDRVLPERLDASYIAEDGEKHRPVMLHRAIFGSYERFIGILIEHFAGKLPVWLAPVQ
                     AVVATIVSDADPYAADVAAQLQAAGIRVETDLRNEKINYKVREHSLAKVPHLLVVGKR
                     EAEEGTVAIRTLGEERQRFMPLAEAIALLKAEATPPDLR"
     gene            complement(3246..3374)
                     /locus_tag="VN627_01825"
     CDS             complement(3246..3374)
                     /locus_tag="VN627_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02024.1"
                     /translation="MAHALALAAQPIRHISAEWLRVAVVSGCAMALILAERALPVF"
     gene            complement(3590..>3962)
                     /locus_tag="VN627_01830"
     CDS             complement(3590..>3962)
                     /locus_tag="VN627_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HXD02025.1"
                     /translation="ARFVGPNRLALPRATGPDDPNAAIYADAKARAEAFGVEVVEVPS
                     PGRVMSGDRVEPASYMNFAVTSKLVVVPIYGTVHDEDGVAAIAELFPDRGTIGLMADA
                     VLMGGGSFHCASQQMPRPTAP"
BASE COUNT          707 a         1482 c         1215 g          558 t
ORIGIN      
        1 tgcgcgcttc cggcggcggc gcgagcggta ccgtcttgtc cgtctgcccg cgcggcttgc
       61 gcaccacgcc ctgctcaacc ggggtcgagc gagacgcccc cggcacggtc gaaaacggcc
      121 cgacctgggc gatcagcggc aaaagtggga gcaacagcgg catcatgatt cctgaccggc
      181 cagttcgccc agcagcgcct gcaccgtgcg cagcagcagg gcaatgtccg cctcgcggct
      241 gaggcgatga tcgccgtcct tgatcaggat cgcctgcacg tcggctgaac gcaaggcccg
      301 gaccagcctc agcgccacgt cccacggcac gtcggcgtcg gcctgcccct gcaacagccg
      361 cgccgggcag tcgagcgcga tttctcccgc cagcagcacg ttgccctgcc catcacgcca
      421 gaacccggga tgggtcggcg tcggctcggg cccatagggg ttgtcctcaa ggagcacctc
      481 gccggtcgcc agcagcgcct gctgcgcttc gtcgaagccc cattcggtaa agtccggcgc
      541 cgcggcaatg cccaccagcc ccgccacgcg ccccggcccg gagtcattca gcgccagtgc
      601 caccagcagc atcagccacc cgcccatcga cgagcccacc agcaccagcc gccgccctgc
      661 gcacagcgcc gccaccagcg ccagcacctc gtcccgccag cgcgaaagcg tgccctccgc
      721 gaaggacccg ccgctctgcc cgcagcctga atagtcgagc agcaggcagg ccctcccggt
      781 cgcccgcgcc cagccaaaca gcgcctcggc cttcgatccg gccatgtcgc tcatgtagcc
      841 cggcaggaat accagcaccg gccccgtccc gtcggtgaag cggtaggcga tccgggggcc
      901 tgcgggcagc tggtgatagg cgatctcggt catgcccggc ctcattcccg gcttgcgccc
      961 gcttggcaag tgcgaccggt ggacgccgcc cggcaccgcc gctatgggcc ttgcccatgt
     1021 ccgagatgat cacgatcagc ctgcccgatg gttcctcccg cgaaatgccg caggggtcca
     1081 ctccgctcga tgtcgccgcc gccatcggcc ccggcctcgc caaggccgcg cttgccgccc
     1141 gcgtcgatgg cgaactggtc gatctcaccc gcccgttcac cggcgatgcg cagctcgccc
     1201 tgatcaccag ccgcgacgag gccgaggcgc tcgaccttgc ccggcacgac tatgcccacg
     1261 tgctggccga agcggtccag gccctgttcc ccgggaccca gatcaccttc gggccgagca
     1321 cggatgacgg tttctactac gattttgccc caaaggaccg gcccttcacc gatgaagacc
     1381 tcccggcgat cgaagcggaa atgcgcaaga tcatcgccgc caacaagccg ctgcgccgcg
     1441 aagtctggtc gcgcgaacag ctgatcagcc ggtggcagca gcagggcgaa agcttcaagg
     1501 ccgaatgggc cgccgaattg ccgggcgatg aggacctgac cgtttattgg tcgggcgacg
     1561 attggctcga catgtgccgc ggcccgcacc tgccctccac cggcaagctc gaccccgcag
     1621 cgttcaagct cacccgcgtc tccggcgcct attggcgcgg cgaccagaag aacgccatgc
     1681 tcagccgcat ctacggcacc ggctggctga gcaagaagca gctcgatgcc cacctcaccc
     1741 ggctggaaga ggctgccaag cgcgatcacc gcaagctggg cgcggagatg gacctgttcc
     1801 acctccagca ggaagcccac ggctcggtct tctggcatcc caacggctac aagatctggc
     1861 gcgagctcga agcctacatg cgccgcgcga tcgatgccgc cggctaccgc gaggtcaaga
     1921 ccccgcaggt catggacgcg cgccagtggg aacagtccgg ccactggggc aagtaccgcg
     1981 agaacatgtt cgtcatcccc gacgaagtgc caaacgtcga tgacgaaggc ccggtgatct
     2041 cgggcgaggc cgactggatg gccttgaagc cgatgaactg cccggcccac gtcctgatct
     2101 tccgccaggg tatcaagtcc taccgcgacc tgccgctgcg catctacgaa aacggctgct
     2161 gccaccgcaa cgaaccccac ggcgcgctcc acgggctgat gcgcgtgcgc cagttcacgc
     2221 aggacgatgc ccacatcttc tgccgcgaag accagatcgt tgcggaagtg cgcgccttct
     2281 gcgaactggc tgaccggatc taccagcact tcggcttcac ctattcgatc aagctcgcgc
     2341 tgcgcccgga aaagcgcttc ggctccgatg ccgattggga caaggccgag ggcgaactca
     2401 ggaacgccgt cgccgaagcc ggcctcgcca cgcccgaata tggctgggag gaactcccgg
     2461 gtgaaggcgc cttctatgcg cccaagctcg agtggcacct gaccgacgcc atcggccgca
     2521 cctggcaggt cggcacgatc cagtcagacc gcgtcctgcc cgagcgcctc gatgcgagct
     2581 acatcgcgga agacggcgaa aagcatcgcc cggtcatgct ccaccgcgcg atcttcggct
     2641 cctatgagcg gttcatcggc atcctgatcg aacacttcgc cggcaagctg ccggtctggc
     2701 ttgccccggt ccaggccgtg gtggcaacca tcgtctccga tgccgatccc tatgccgcgg
     2761 acgtcgccgc ccagctccag gccgccggca tccgcgtcga aaccgatctc aggaacgaga
     2821 agatcaacta caaggtgcgc gagcacagcc ttgccaaggt tccgcacctg ctggtcgtcg
     2881 gcaagcgcga agccgaggaa ggcaccgtcg ccatccgcac cctcggcgag gaacgccaga
     2941 ggttcatgcc gctggccgaa gccatcgcgc tgctcaaggc cgaagccacc ccgcccgacc
     3001 tgcgctgacc aaaatcctcc ccgctcgcgg ggaggtggca gccgccaagc ggctgatgga
     3061 gggagcgtgc cacaagcacc gcccctcctc cgcacgtcac cctgaacgcg tttcaggacc
     3121 catggcgcaa cgtcccgacc cgctgccagg cccgaggcag gacgcaaggt cagcccatgg
     3181 atgctgaacc gggttcagca tgacgaagct ttgatcagga aaaagtcggg tcgggcccaa
     3241 ccggctcaaa aaaccggcag cgcccgctcg gccaggatca gcgccatcgc acagccgctg
     3301 acgaccgcca cccgcagcca ttctgcggaa atgtggcgaa tcggttgagc ggcaagggca
     3361 agcgcatggg ccatgcacca aggaatgcgc cgcaagtccc taccggcggg ttaacgcggg
     3421 tccggtcaaa cccactccgc tgcggctacc tttgcctccg gctcagcacg ttttccgacc
     3481 cgcccgcctg cgcgagggct gaacacggcg ccccctgctc ccctcccgca ggcgggaggg
     3541 gcagcgagac ttgggtgcga agcgccctgg tcgcagcggg gtgggcatct cacggcgcag
     3601 tcggtcgcgg catctgctgg ctcgcacaat gaaagctccc gccccccatc agcacggcat
     3661 cggccatcag cccgatggtc ccccggtccg gaaacagctc ggcaatcgcc gccacgccgt
     3721 cctcatcatg caccgtcccg tagatcggca cgaccaccag cttcgaagtc acggcaaagt
     3781 tcatgtagct cgccggctcc acccggtcgc cgctcatcac gcgcccgggc gaaggcacct
     3841 cgaccacctc caccccgaag gcctctgccc gggccttggc atcggcatag atcgccgcgt
     3901 tgggatcgtc cggcccggtc gcgcggggca gggccagccg gttcgggccg acgaaccggg
     3961 cg
//
LOCUS       DATNBV010000077         5062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1007560,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000077 DATNBV010000000
VERSION     DATNBV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007560"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..781)
                     /locus_tag="VN627_01835"
     CDS             complement(80..781)
                     /locus_tag="VN627_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HXD02026.1"
                     /translation="MYRVVTAVLGLCMALAGCSRGSPIPEGSASETVATAPAKDAVPV
                     MGPERRILAVGDSLLAGYGLASPKEAYPARLETALRARGINALITNAGVSGDTSSAGA
                     ERFGFVLKSQPAKPDLVIIEFGGNDILRAVPPEQTRANLDRMLGQARAAGLPVLLMGM
                     LAPPNLGPDFKAKFEPIYPELAKRHGARLVPFFLAPVMGKPDLVQADHIHPTAGGVDE
                     IVAATVGDVAQALPK"
     gene            851..1510
                     /locus_tag="VN627_01840"
     CDS             851..1510
                     /locus_tag="VN627_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXD02027.1"
                     /translation="MIRASGLTLTLGQTRILKGIDLAVAPGETLALLGPSGSGKSSLM
                     ALLTGLERASGGLLEVAEADFSALDEDQLARARRGRIGIVLQAFHLLPTMTARENVAT
                     PLELAGEPDAQARAAEELAAVGLGHRLDHYPAQLSGGEQQRVAIARALAPRPPLLFAD
                     EPTGNLDAATGAAIIDLLFARREVTGATLVIITHDADLARRCGRVITLADGRIASDVR
                     G"
     gene            1641..4178
                     /locus_tag="VN627_01845"
     CDS             1641..4178
                     /locus_tag="VN627_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXD02028.1"
                     /translation="MSATLPWPTAWRIARADLSWRFRGLRLLLACLFLGVAALAAIGT
                     LTGSIERELTSRGRMILGGDIGVEVWQRDLTAPELSALKALGTVSLGTRLQAMAAAGD
                     ATVPVQLKAVDDKWPLVGRLSLTDGRKVGAPPPGQAWLAEGAAERLGLAPGQRFTIAG
                     RPLMVGGIIADEPDRLGEGFSWGIPVIVRSDFPRAAGLTATGSQFRTRAAVAFNADYD
                     PAAATASLQRRFPQAGFTLRTRDKAAPGTETFIDRMGQFLVLVGLAALVIAGIGIGLG
                     LASWLEGRRASIATLKVLGATSGDIARITLLQVAAASAVAIGAGLAVGVLATPLLASA
                     LGGLLPVSPGLVIDGKALAVAAVYGLLVALVFAAPPLARARAFPAMALMRARVSPLAL
                     PWRHAALPVLIGLAAIVALALATSAQPLVTLGFLGGAAGTLALLALLGLALRRLAARL
                     PRPRGALLRTALANLHRPGSQVPALVTALGFGLSAFVLLAAVQTAMSANIAARVPQRA
                     PDFFVLDVPRERAAEFRAAVAVTAPGAQVRMVPNLRGAILAYGPPGRLARVADQPGAA
                     DKSWALRGERGLTYADAVPEGNTLTAGQWWPKNYAGPPLVSVDEKLADAIGLRLGDRI
                     AVSLLGVEREATVASFRRIDWDSMGFNYVLVFSPNMLADAPHNLAATIDLPASPDGVK
                     RRPPGLLRALVRAFPAASVIETGPILRDARGLLDQVSLAILAAAGVAVLAGIAVLIGA
                     IAAARAARTYDTAVLRVLGASRRQVLLVLLAEYGFIAGLLALIALPLGSAVAWWIIVR
                     LFDFEWLPDWPRVLAVLAGGLALVLGFAMAATLPVLRARPAQVLREL"
     gene            complement(4358..4771)
                     /locus_tag="VN627_01850"
     CDS             complement(4358..4771)
                     /locus_tag="VN627_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HXD02029.1"
                     /translation="MEWMILPYRRYAEFTGRSRRKEYWMFVLFQVLVSLAIGVVFGRT
                     SYMTGAGMMTGNVMVQGPGGMVQNLFSLVSLIPGIAVSVRRLHDQDRSGWLLLLALIP
                     FLGWFALLVFMCLEGTRGPNRFGPDPKNPTDLEAF"
     gene            complement(4805..>5062)
                     /locus_tag="VN627_01855"
     CDS             complement(4805..>5062)
                     /locus_tag="VN627_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HXD02030.1"
                     /translation="WLGIGLLGLVALAMLVPSVAVTVRRLHDRDMSGWWYLGVIIVGA
                     IPFVGFLANIAFLVLMCLPGTPGPNRFGPDPKGTDSYEAFA"
BASE COUNT          697 a         1973 c         1605 g          787 t
ORIGIN      
        1 aagcccctcg tcagcctgaa agccccctcc gtcagccctg ccgggctgcc acctccccac
       61 tcgtggggag gaattccgcc tatttcggca gcgcctgcgc aacgtctccc accgtggccg
      121 ccacgatctc gtcgaccccg ccggcggtcg gatggatatg gtcggcctgc accaggtccg
      181 gcttgcccat caccggcgcg agaaagaacg gcaccagccg ggccccatgc cttttcgcca
      241 gttccggata gatcggctcg aacttcgctt tgaaatcagg gccaaggttc ggcggggcga
      301 gcatgcccat gagcagcacc ggcaggcccg ccgcccgcgc ctgccccagc atccggtcaa
      361 ggttcgcgcg ggtctgctcg ggcgggacgg cgcgcaggat gtcgttgccg ccgaactcga
      421 tgatcaccag gtcgggcttg gccggctggc tcttcagcac gaaaccgaac cgctcggccc
      481 cggcgctgga ggtgtcgccc gaaaccccgg catttgtgat caaagcattg attccccttg
      541 cgcgcagcgc cgtctccagc cgtgccggat aggcctcctt cggactggcg aggccgtagc
      601 cggcgagcag gctgtcgccc acggcaagga tgcggcgctc cgggcccatc accggcacgg
      661 cgtccttcgc cggcgccgtg gccacggtct cgctggcgga accctcagga atggggctcc
      721 cgcgcgaaca ccctgcaagc gccatgcaca ggcccagcac cgccgttaca accctgtaca
      781 ttttgcctcc ttgccgctcc gcccctagct atgccatccc ccgccggtga caagcccccc
      841 acagccgccg atcatccgcg ccagcggcct caccctcacc ctggggcaaa cccggatcct
      901 caaggggatc gaccttgccg tcgcccccgg cgagacgctg gccctgctcg gcccctcggg
      961 ctcgggcaaa tcgtcgctga tggcgctgct gaccgggctg gagcgggcat cgggcggctt
     1021 gctggaagtg gccgaggccg atttctccgc gctcgatgaa gaccagctgg cccgggcgcg
     1081 gcgcggccgg atcgggatcg tgctccaggc ctttcacctc ctgccgacga tgaccgcgcg
     1141 ggagaatgtt gccacgccgc tcgagctcgc cggcgagccc gatgcgcagg cccgtgcggc
     1201 ggaggagctc gccgccgtcg gcctcggcca ccggctcgat cattaccccg cgcagctttc
     1261 gggcggcgag caacagcgcg tggccatcgc ccgcgccctc gccccgcgcc cgccgctgct
     1321 cttcgccgac gagccgaccg gcaaccttga tgcggccacc ggcgccgcca tcatcgacct
     1381 gctcttcgcc cggcgcgaag tgacgggggc gacgctggtg atcatcactc acgatgccga
     1441 cctcgcccgc cgctgcggcc gtgtgatcac cctcgccgac ggccggatcg ccagcgatgt
     1501 ccggggctga agccgatgct acgccggcca ctcccctccc gcaggcggga ggggctgggg
     1561 gtgggcaggc ggccagccct tcgcctgccg gcatgcccaa ccctacaccc tcccgcctgc
     1621 gggaggggaa cttgccccac atgtccgcta ccctcccctg gcccaccgcc tggcgcattg
     1681 cccgggccga cctgtcatgg cgcttccgcg gtctcaggct gctgctagcc tgcctgttcc
     1741 tcggcgtcgc cgctcttgcc gccatcggca cgctgaccgg ctcgatcgag cgcgagctga
     1801 ccagccgggg ccgcatgatc ctgggcggcg atatcggcgt cgaagtctgg cagcgcgatc
     1861 ttaccgcgcc cgaactttcc gctctgaaag ccctcggcac cgtcagcctc ggcacccgcc
     1921 tccaggccat ggcggcggcc ggcgatgcca cggtcccggt ccagctcaag gcagtcgatg
     1981 acaagtggcc gctcgtcggc aggctctcgt tgaccgatgg ccgcaaggtc ggcgcgccgc
     2041 cgcccggaca ggcctggctg gctgaaggcg ccgccgaacg gctcggcctt gcgccgggcc
     2101 agcgcttcac catcgccggc cggccgctca tggtcggcgg catcatcgcc gacgagcccg
     2161 accggctggg cgaaggtttc tcctggggca tacccgtcat cgtccggtcc gatttccccc
     2221 gcgccgccgg gctcaccgcc accggctcgc agttccgcac ccgcgccgcc gtggccttca
     2281 atgccgacta tgatccagcc gctgccaccg ccagcctcca gcgccgcttc ccgcaagccg
     2341 gcttcaccct gcgcacgcgc gacaaggccg cccccggcac cgagactttc atcgaccgga
     2401 tgggtcagtt cctggtcctc gtcggcctcg ccgccctcgt gattgccggg atcgggatcg
     2461 gacttggcct cgcctcatgg ctggagggcc gccgcgcctc gatcgcgacg ctcaaggtcc
     2521 ttggcgcgac cagcggcgac attgcccgga tcaccctgct gcaagtcgcc gcagcctccg
     2581 ccgtcgccat cggcgcgggc ctcgccgtgg gcgtcctcgc cacgccgctt ctcgccagcg
     2641 ccttgggcgg cctgctcccg gtcagcccgg gactggtgat cgacggcaag gccctcgccg
     2701 tcgccgcggt ctacggcctg ctcgtcgccc tcgtcttcgc cgccccgccg cttgcccgcg
     2761 cccgcgcctt tccggccatg gccctgatgc gcgcgcgggt cagcccgctt gccctgccgt
     2821 ggcggcatgc ggcgctgccg gttctgatcg gccttgccgc gatcgttgcc ctcgcccttg
     2881 ccacctcggc ccaaccgctg gtcaccctgg gcttcctcgg cggggccgcg ggcaccctcg
     2941 ccctactggc cctgctcggt ctggccctgc gccgcctcgc cgcccgcctg ccccgcccgc
     3001 gcggggccct gctgcgcacg gcgctcgcca acctccaccg tcccggcagc caggtccccg
     3061 ccctcgtcac cgcgctcggc ttcggcctct ccgccttcgt tctccttgcc gcggtgcaga
     3121 ccgcgatgag cgccaacatc gccgcccgcg tcccccagcg cgcacccgat ttcttcgtgc
     3181 tcgacgtgcc ccgcgaacgc gccgccgagt tccgcgcggc cgtcgccgtg actgcccccg
     3241 gcgcacaggt gcgcatggtg ccaaacctgc gcggtgcaat cctcgcctat ggcccgcccg
     3301 gccgtctggc gcgcgtggcc gaccagcccg gcgctgccga taaaagctgg gccctgcgcg
     3361 gcgagcgcgg gctgacctat gccgatgccg tgcccgaggg aaacaccctc accgccgggc
     3421 aatggtggcc gaagaactac gccggcccgc cgctcgtttc cgtggacgaa aagctggccg
     3481 acgccatcgg cctgaggctg ggcgaccgca tcgccgtcag cctgctgggt gtggagcgcg
     3541 aggccaccgt cgcctcgttt cggcggatcg actgggattc aatgggcttc aactatgtcc
     3601 tcgtgttcag cccgaacatg ctggccgacg ccccccacaa cctcgccgcc acgattgacc
     3661 tgccggccag cccggacggt gtgaaacgcc gcccgcccgg cctgctgcgc gccctcgtcc
     3721 gcgcttttcc ggccgcgtcg gtgatcgaaa ccgggccgat cctgcgcgac gcgcgcggcc
     3781 tgctcgatca ggtcagcctc gccatcctcg ccgccgccgg ggtggccgtg ctggccggca
     3841 tcgccgtcct catcggcgcc atcgccgccg cccgcgcggc gcggacttac gacacggccg
     3901 tgctgcgtgt gctcggcgcc agtcgccgtc aggtcctgct ggtgctcctc gccgagtacg
     3961 gctttatcgc cggcctgctc gccctcatcg ccctcccgct cggcagcgcc gttgcctggt
     4021 ggatcatcgt gcgcctgttc gatttcgaat ggctgcccga ctggccccgt gtgctggccg
     4081 tcctggcagg cggcctggcg ctcgtcctcg gcttcgccat ggcggcaacc ctgcccgtgc
     4141 tgcgcgcaag gccggcgcag gttttgcggg agctttagca gggggttcac gcagagtacg
     4201 cagagaagaa ggaggagacg cagagggcga agcgccaccg ggctgcgcag cggccctgtt
     4261 cgtaagacct gcgctctcgc catgcagcac ggcggctccg ccgcaagcta cctctcggcg
     4321 cctctctttt tcctctgcgc ctctgcgtga accaaaatca gaacgcttca agatcagtcg
     4381 gattcttcgg atccggccca aaccggttgg gcccccgggt gccctcgaga cacatgaaca
     4441 ccagcaacgc gaaccagccg aggaacggga tcagcgccag cagcaagagc cagcccgacc
     4501 ggtcctgatc atgcagccgc cgcaccgaga cggcgatgcc cgggatcagg ctcaccagcg
     4561 agaacaggtt ctggaccatg ccccccggcc cctgcaccat gacgttgccg gtcatcatac
     4621 cagcgccggt catgtaggag gtgcgcccga acaccacgcc gatcgccagc gagacgagca
     4681 cctggaacag cacgaacatc cagtattcct tgcgccgcga gcggccggtg aattcggcat
     4741 agcggcgata gggcaggatc atccattcca tggcggtact cctcgctggc gggacggggg
     4801 tggttcaggc gaaggcctcg tagctgtcgg tgcccttggg atcgggcccg aagcggttcg
     4861 ggcccggggt tccggggaga cacatcagca cgaggaaggc aatgttcgca aggaagccca
     4921 cgaaaggtat cgcgccgacg ataatgactc ccaaatacca ccagccggac atgtcgcgat
     4981 catggaggcg cctgacagtc actgccaccg acggcaccag catggccaac gccaccagcc
     5041 cgagcaatcc tatgcccagc ca
//
LOCUS       DATNBV010000078         2384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1639304,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000078 DATNBV010000000
VERSION     DATNBV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639304"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VN627_01860"
     CDS             complement(<1..1038)
                     /locus_tag="VN627_01860"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HXD02031.1"
                     /translation="MALTETQIMDALRAIRLPQGGDIVAAGMVSGLMLRDGHVGFAIE
                     VDPAQAPVMEPVRKAAEKAVMDLPGALSATVVLTAHRAAPQTAQRPTGPAQPQGHGHA
                     HAHGQPGGGRLSLPGIKHIIAVASGKGGVGKSTTAVNLALALHANGLTVGLLDADVYG
                     PSQPRMMGITGKPTSKDGKVMEPMANHGIKVMSMGFLIDEETPMIWRGPMVMSAITQL
                     LRDVAWGELDVLVCDLPPGTGDTQLTMSQNVPLSGAIIVSTPQDIALLDAKKGLNMFR
                     KVDVPVLGIIENMSYFSCPHCGGRSEIFSHGGARREAERFNVEFLGEIPLDIAIRETS
                     DGGQPIVVSQPK"
     gene            1284..>2384
                     /gene="hflK"
                     /locus_tag="VN627_01865"
     CDS             1284..>2384
                     /gene="hflK"
                     /locus_tag="VN627_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HXD02032.1"
                     /translation="MAWNNQGGPWGGGGGQGGGQSPWSRGPGGGGQPPDFEEMLRRGQ
                     DRMKNLVPGGMGPGKVGALIAIVAVLLWAASGLYQVQPAERGVPLVFGKYTGELTAPG
                     LHWNWPGPIGSVLTPNVDAQNTVEIGFRSTGTSNTSFDEESLILTGDENIIQVEAVVQ
                     WFIDDVEKYLFAIRDPNETLSPDALKQDTVKNAAEAAIRETIGQSPFEVVITGEGRED
                     VGKRAQALAQHILDTYNSGIKIERLSLQRSYAPQEVADAFRDVVRAGADAQSTVNDAT
                     AYKNQVTQQALGQASQIKNQAEGYRAEKIAVASGDAQRFIAVYDQYKQNPAVTSRRLY
                     LETLEQIMGNMSKVLIDTGQGGAVPYLPLDQLL"
BASE COUNT          427 a          742 c          814 g          401 t
ORIGIN      
        1 cttgggctgg ctgaccacga tcggctggcc gccatccgag gtttcgcgga tcgcgatgtc
       61 gagcgggatc tcgcccagga actcgacgtt gaacctttcc gcttcgcgcc tggcgccgcc
      121 atgggagaag atttcgctgc gcccgccgca atgggggcag gagaaatagc tcatgttctc
      181 gatgatgccg aggaccggca catcgacctt gcggaacatg ttgaggccct tcttggcgtc
      241 aagcagagcg atgtcctgcg gggtcgagac gatgatcgca ccggagagcg gcacgttctg
      301 cgacatggtg agctgggtat cgccggtgcc gggcggcagg tcgcacacca gcacatccag
      361 ctcgccccag gcgacgtcgc gcagcaattg cgtgatcgcg ctcatcacca tgggcccgcg
      421 ccagatcatc ggcgtctctt cgtcgatcag gaagcccatc gacatcacct tgatgccgtg
      481 attggccatc ggctccatga ccttgccgtc cttgctggtg ggcttgccgg tgatgcccat
      541 catgcgcggc tgcgaggggc catagacgtc ggcatcgagc agcccgaccg tgaggccatt
      601 ggcatgcagc gcgagcgcga gattgacggc ggtggtcgac ttgccgaccc cacccttgcc
      661 gcttgccacc gcaatgatgt gcttgatgcc gggcagcgag aggcggccgc cgcctggctg
      721 gccatgagcg tgcgcatggc catgcccttg gggctgggcc gggccggttg gccgttgcgc
      781 ggtttgcggg gcggcgcgat gggcggtcag taccactgtc gcgctcagcg ctcccggcag
      841 gtccatgaca gccttttcgg ccgccttgcg gaccggctcc atcaccgggg cctgcgccgg
      901 gtcgacttcg atggcaaagc cgacatggcc gtcgcgcagc atcaggccgc tgaccatccc
      961 ggccgccacg atatcgccgc cttgcggcag ccgaatggcg cgcaatgcgt ccatgatctg
     1021 ggtttcggtg agggccatgc ttgaaacacc tttggcggcg gacgatatgc gagagacggc
     1081 tcggcccggc ccgccgacgg gggcatccaa gggcgaggtc caaggagggt ttacccacgt
     1141 tgcgcgaccg gcgcgcccct gtcatataag gccgcgggtc ggcgacgcaa ttcatagatt
     1201 gcggccggtg cggagaatca tggcgggagg tctggtttcc gcctcaacga acataaattt
     1261 tccgggaata atctggagtt atcatggctt ggaacaatca gggcggccca tggggcggtg
     1321 gcggcggtca gggcggcggg cagagcccgt ggtcgcgcgg ccccggtggt ggcggccagc
     1381 cgcccgattt cgaggagatg ctgcgccgcg gccaggaccg catgaagaac ctggttcccg
     1441 gcggcatggg ccccggcaag gttggcgcct tgatcgccat cgtggcggtg ctgctgtggg
     1501 cggcgagcgg cctttaccag gtgcagccgg ccgagcgcgg cgtgccgctg gttttcggca
     1561 agtataccgg agaactgacg gcacctgggc tgcactggaa ttggccgggg ccgatcggca
     1621 gcgtgctgac gccgaacgtc gatgcccaga acaccgtcga gatcggattc cggagcaccg
     1681 gcaccagcaa caccagtttt gacgaagaaa gcctgatcct gaccggggac gagaacatca
     1741 tccaggtcga ggcggtggtg caatggttca tcgatgacgt ggagaagtat ctgttcgcga
     1801 tccgcgatcc gaacgagacg ctgtcgccgg acgccctgaa gcaggacacg gtgaagaacg
     1861 cggccgaggc cgcgattcgc gagacgatcg gccagtcgcc ctttgaagtg gtcatcaccg
     1921 gcgagggccg cgaagatgtc ggcaaaaggg ctcaagcgct ggcgcagcac atcctcgaca
     1981 cctacaactc tggcatcaag atcgagcgcc tgtcgctcca gcgctcctat gcgccgcagg
     2041 aagtggccga cgcgttccgg gacgtcgtgc gcgccggtgc cgacgcgcag tcgaccgtga
     2101 acgatgccac cgcctacaag aaccaggtga ctcagcaggc gcttggccag gcgagccaga
     2161 tcaagaatca ggccgaaggc tatcgcgccg agaagatcgc ggtggcgagc ggcgatgcgc
     2221 aacggttcat cgcggtctac gatcaataca agcagaaccc cgccgttacg tcccgccgtc
     2281 tctatctgga gacgctggaa cagatcatgg gaaacatgag caaggtcctc atcgacaccg
     2341 gccaaggcgg cgcggtgcca taccttccgc ttgaccagct cttg
//
LOCUS       DATNBV010000079         2805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_57653,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000079 DATNBV010000000
VERSION     DATNBV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57653"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1560)
                     /gene="glpD"
                     /locus_tag="VN627_01870"
     CDS             complement(34..1560)
                     /gene="glpD"
                     /locus_tag="VN627_01870"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HXD02033.1"
                     /translation="MALDRTDYDLLVIGGGINGVGIARDAAGRGLRVLLVERDDLAGH
                     TSSASTKLIHGGLRYLEYYEFRLVREALRERERLLGIAPHIAWPLRFVLPRPEGGRPG
                     WLIRLGLFIYDRLGGRQTLPRSQAVDLADPRWGAGLRPGLRRGFAYSDAWVDDARLVV
                     LNAMDAAERGATILTHTAFTGARVDGDAWLAELATAHGAQSLRARVIVNAAGPWVADI
                     IDGLPGVRREGGVALVKGSHIIVPRLYPGDHAFLLQQPDGRIVFTIPYQHRYTLIGTT
                     DVVVGQAERAAPRITSAEVDYLCAAVSPYFTQDVTPASVIATYSGVRSLHDDGKADAK
                     AITRDYVLQLGRAAGPQVLSVLGGKLTTYRRLAEHAHAKLAPWLPEAGPAWTASAPLP
                     GGDLPPGGFAAFLGQVRARWPFLPPDVSERMAHAYGTRLAAIIGAASAWDQLGEDFGS
                     GLTEAELRYLADHEWATCADDVLWRRTKLGLAAPPDLAERVAGWFAARAMGKTGRAGA
                     "
     gene            complement(1735..>2805)
                     /locus_tag="VN627_01875"
     CDS             complement(1735..>2805)
                     /locus_tag="VN627_01875"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD02034.1"
                     /translation="VEGRTKLAINGHISDYIPNPTFEVVAAPGSHEIWFRGENSEGKS
                     LRELTGDPLAFQPEFAEPESRLKLLDEQRVHAQLVFPTLASVIEGHMGNNIELMAACV
                     HSLNAWIADEWTFHYKDRLFPCAYISLADADLACAELDWALKQGARHILIRPAPVPGR
                     FGPRSPGLPEFDKFWARVNESNIFVVLHVSDSGYDEVYRWWTGGSEMLAFDRVDPLKS
                     CIDTQGRAIADMISALIAHGVMSRFPNIRWVSAENGSIWVPHLMKMLKRAYGQMPKSF
                     TRHPVETFQECVYVAPYYEEDLNELRQHVAADRLLFGSDWPHAEGLELPLDFLNEVKD
                     FTPEDQKMFMSSNLKGLLEGKR"
BASE COUNT          455 a          904 c         1015 g          431 t
ORIGIN      
        1 ttgtgccctg gtcgatggcc aggatgatgc cgctcatgcc cctgcccttc cggtcttgcc
       61 catggcccgc gccgcgaacc agccggcgac gcgttcggcg aggtcgggcg gggcggcgag
      121 gccgagcttg gtccgccgcc acagcacgtc atcggcgcag gtggcccatt cgtgatcggc
      181 gaggtagcgc agctccgcct cggtgaggcc gctgccgaag tcctcgccca actggtccca
      241 ggcggacgca gcgccgatga tggcggccag ccgcgtgccg taggcgtggg ccatgcgttc
      301 ggagacgtcg ggcggcagga atggccagcg ggcgcggact tggccaagga aggcggcgaa
      361 cccgccgggc ggcaggtcgc cgccggggag cggggccgag gcggtccagg ccgggccggc
      421 ttcgggcagc cacggggcga gcttcgcgtg ggcatgttcg gcgaggcggc ggtatgtcgt
      481 cagcttgccg ccgagcacgc tcagcacctg cgggcccgcc gcgcggccca gctggaggac
      541 atagtcccgg gtgatcgcct tggcgtctgc cttgccgtcg tcatggagcg agcgcacgcc
      601 cgaataggtg gcgatgactg aggccggggt gacgtcctgc gtgaaatagg ggctgaccgc
      661 ggcgcagaga taatcgactt ccgccgaggt gatccggggc gcggcccgtt cggcctggcc
      721 gaccacgacg tcggtcgtac cgatcagggt gtagcggtgc tgatagggaa tggtgaagac
      781 gatccgcccg tcaggctgct ggaggaggaa ggcatgatcg cccggataga ggcgcggcac
      841 gatgatgtgg ctgcccttga cgagggccac gcccccttcc cgccgcaccc cgggcaggcc
      901 atcgatgata tcggcgaccc acggcccggc ggcattgacg atgacccgcg cccggaggct
      961 ttgcgcaccg tgggccgtgg ccagttcggc cagccaggca tcgccgtcta cccgcgctcc
     1021 ggtgaacgcg gtgtgggtaa ggatcgtcgc cccgcgctcg gcggcgtcca tggcattgag
     1081 caccaccagc cgggcatcgt ccacccaggc gtcggaatag gcaaagccgc gcctgaggcc
     1141 ggggcgcagg ccggcgcccc agcggggatc ggcgaggtcc accgcctgtg agcgcggcag
     1201 ggtctgcctg ccgccgaggc gatcgtagat gaacaggccg aggcggatca gccagccggg
     1261 gcgcccgccc tccggccggg gcaggacgaa gcgcaggggc caggctatat gcggtgcgat
     1321 cccgagcagc cgctcgcgct cgcgcagggc ctcgcggacg aggcggaact cgtagtattc
     1381 gagatagcgc aggccgccgt gaatcagctt ggtgctggca gacgaggtat ggccggcaag
     1441 atcatcgcgc tccaccagca gaacccgcag gccgcgtccg gccgcatcgc gggcgatccc
     1501 cacgccgttg atcccgccgc cgatgacgag gaggtcatag tcggtccggt ccagcgccat
     1561 gcctgctccc ccggttcgcc ggtggaacgg gcgaacgtgt taccctcctg catatgcgcc
     1621 ttgccgaaga gggtgaagtc ctcgtggcaa ccgccgccgg cctcgcgcac aaaaaatgca
     1681 gccgggcgcg gggcccggct gcacaattgc cgctggctgg ggcgtcaggc ccgatcagcg
     1741 cttgccttcg agcaggccct tgaggttgga gctcatgaac atcttctgat cttcgggcgt
     1801 gaagtccttg acctcattga ggaaatcaag cggcagttcg agaccttcgg catgaggcca
     1861 gtcgctgccg aacagcaggc ggtcagccgc aacgtgctgc cgcagttcgt tgagatcttc
     1921 ctcgtagtag ggcgcgacat agacgcattc ctggaaggtc tcgaccggat ggcgggtgaa
     1981 gctcttgggc atctggccgt aggcgcgctt cagcatcttc atcaggtgcg gaacccagat
     2041 cgagccgttc tcggccgaga cccagcggat gttggggaag cggctcatca ccccgtgggc
     2101 gattagcgcc gagatcatgt cggcaatggc gcggccctgt gtgtcgatgc acgacttgag
     2161 cggatccacg cggtcgaacg cgagcatttc cgagccgccg gtccaccacc gatagacctc
     2221 gtcatagccg gaatccgaca cgtggaggac cacgaagatg ttggattcgt tcacccgggc
     2281 ccagaatttg tcaaactccg gcaggcccgg cgaacggggg ccgaaccggc cggggaccgg
     2341 ggccggacgg atcaggatgt gccgcgcgcc ctgcttgagc gcccagtcca gctccgcgca
     2401 ggccaggtca gcatcggcca gcgagatata ggcgcagggg aacaggcggt ccttgtagtg
     2461 gaaggtccac tcgtcggcga tccaggcgtt gagcgagtga acgcaggcgg ccatcagctc
     2521 gatgttgttg cccatgtggc cttcgatcac cgaggccagg gtggggaaca ccagctgggc
     2581 gtggacccgc tgctcatcca gcagcttgag gcgggattcc ggttcggcga actcgggctg
     2641 gaaggccagc ggatcgccgg tcagctcgcg cagcgacttg ccctccgagt tctcgccccg
     2701 gaaccagatc tcgtgcgaac cgggcgcggc cacgacctcg aaggtggggt tggggatgta
     2761 gtccgagata tggccgttga tcgccagctt ggtgcggcct tccac
//
LOCUS       DATNBV010000080         3882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2603208,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000080 DATNBV010000000
VERSION     DATNBV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2603208"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VN627_01880"
     CDS             <1..285
                     /locus_tag="VN627_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8"
                     /protein_id="HXD02035.1"
                     /translation="DSLALRVSRPLRVTSGGLSLLLPEAWNYATARPTMALERLALVP
                     SGRETDAELAWHGLLWGGEASASAYWRDQPGHVASLPAEKGVAISWAKGF"
     gene            complement(315..950)
                     /gene="gmk"
                     /locus_tag="VN627_01885"
     CDS             complement(315..950)
                     /gene="gmk"
                     /locus_tag="VN627_01885"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXD02036.1"
                     /translation="MASDTLHRRGLMFILSSPSGAGKTTIARRLLAEDREIAMSVSAT
                     TRPMRPGEVEGKDYHFVSQPEFDRMVEANEFYEWAHVFGHSYGTPKSHIRAALKDGQD
                     FLFDIDWQGTQQLYQKAERDVVRVFILPPSLDELQRRLVGRGTDSAEIIAARMARAQA
                     EISHWDGYDYVVVNDDVEACFGKVREILAAERMRRTRQTGLIDFVRELTRS"
     gene            complement(1021..1446)
                     /locus_tag="VN627_01890"
     CDS             complement(1021..1446)
                     /locus_tag="VN627_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02037.1"
                     /translation="MTMAFLRKRSKAVVKAEDAMDQAPDLAIAAATPSPNPATNLLIA
                     DVALRGGGALLKRAVEAGLLGATATSKRKAKDVVKGRSLGQSLVGTMIARVATRSVPG
                     ALIVGGGLLAKTLYDRKRARSAKAEGHEKLDELARKGRS"
     gene            complement(1468..1971)
                     /locus_tag="VN627_01895"
     CDS             complement(1468..1971)
                     /locus_tag="VN627_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HXD02038.1"
                     /translation="MNDTPDSLIPYDEIVQEALRAVVGRVLGQVEAGGGMLPGHHHFY
                     ITFKTGAPGVEIPTQLRERFPDEMTIVLQNKFWDLSVGEDSFSVGLSFNQVPAKLLIP
                     FAAITAFVDPAVDFGLQFQATVNDLAPEPHDDAENDAPDGEEHGGETGVAEDGSNVVT
                     IDFGRKK"
     gene            complement(2061..3422)
                     /locus_tag="VN627_01900"
     CDS             complement(2061..3422)
                     /locus_tag="VN627_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXD02039.1"
                     /translation="MSSATSPSARHPRYRRLLAPVALALALGLLPGAPGPTLAQAQAV
                     APSMRAVLADRAPGDLRAFYAARGWQPLWLDEAGRVRQAAFLLLGQVEQARMDGIRQN
                     RLRAGDLRKALDRLGNGEPANLARVEFAASRVYANWVTALRSAPHAHMQYESEALAPA
                     VPTQSAALAAAAKAPSLDQFVAEMGWMHPWYAPLRAALLSHDYDDEQRHQIALNLDRL
                     RALPPYPADRYVLVDAAGARLWMFEHGRPVDSMKVVVGKPDNQTPMMAGFIRYAILNP
                     YWNVPEDLVRQRIAWNVLEKGLTYLKTGGYQVLPDWDASAPMDPSLVDWQAVEDGRAT
                     VVVRQLPGGSNFMGRVKFEFPNPQGIYLHDTPEKALMRKDERTASSGCVRLEDAQRLG
                     RWLMGKPLPRKVAEPEKRMNLPQLVPVYITYLTVFPQKTGGLAFRSDPYGRDEGLTQL
                     AVR"
     gene            complement(3427..>3882)
                     /locus_tag="VN627_01905"
     CDS             complement(3427..>3882)
                     /locus_tag="VN627_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HXD02040.1"
                     /translation="VAALAGGDVLAGAAIGAAVGGIAGAVWADRDGDGRVDGYVREGQ
                     YYPGVPEGYDPNYQSAAAAAPACRSVGGTAVTGAAVGGVGGAAVGALAGNVLAGAVLG
                     AAVGGLAGAVWADHNNDGCVDGYVREGRYYEGAPVIQPTDNYRRTGERG"
BASE COUNT          585 a         1259 c         1415 g          623 t
ORIGIN      
        1 gacagcctgg cgctgcgtgt ttcccggccg ctgcgggtga cttcgggcgg gctctcgctg
       61 ctgctgcccg aagcatggaa ctatgccacg gcccggccga ccatggcgct ggagcggctg
      121 gcgctggtgc cgagcgggcg cgaaaccgac gccgagctgg cctggcacgg cttgctgtgg
      181 ggcggcgagg cttcggccag cgcctactgg cgtgaccaac ccggccacgt ggcgagcctg
      241 ccggcggaga agggcgtggc gatcagctgg gcgaagggtt tctagagcgt gatcaagatg
      301 atcacgctcc aagcctagct gcgggtcagt tcgcgcacga agtcgatcag cccggtctga
      361 cgggtgcggc gcatgcgttc ggccgcgagg atctcgcgga ccttgccgaa gcaggcttcg
      421 acgtcgtcat tgacgaccac gtagtcgtag ccgtcccagt ggctgatctc ggcctgggcg
      481 cgggccatgc gtgcggcgat gatctcggcg ctgtcagtgc cccggccgac caggcggcgc
      541 tgaagttcat cgaggctcgg cgggaggatg aagacacgca ccacgtcgcg ctcggccttc
      601 tggtagagct gctgtgtgcc ctgccagtcg atgtcgaaca ggaagtcctg cccatccttg
      661 agcgcggcgc ggatgtggct cttgggcgtc ccgtaggagt ggccgaaaac atgggcccac
      721 tcgtagaact cgttggcttc gaccatgcgg tcgaattcgg gctggctgac gaagtggtag
      781 tccttgccct cgacctcgcc cggacgcatc ggccgggtcg tggccgagac cgacatggcg
      841 atctcgcggt cctcggccag caggcggcgg gcgatcgtgg tcttgcccgc gcccgagggc
      901 gaggacagga tgaacatcaa cccgcggcgg tgaagcgtgt cggaagccat ggctgccctt
      961 ggcgcaaacc ggggcggcag atcaaggggg cgcggcgagt tctccgccgt gggcgcggct
     1021 tcagcttcgg cccttgcggg cgagctcgtc gagcttttca tgaccttcgg cctttgccga
     1081 acgggcgcgc ttgcgatcgt agagggtctt ggcgaggagg ccgccgccga cgatcagggc
     1141 gccggggacc gagcgcgtgg cgacgcgggc gatcatcgtg ccgaccaggc tctgcccgag
     1201 cgagcggccc ttgaccacgt ccttggcctt gcgcttgctg gtggccgtcg cgcccagcag
     1261 cccggcctcg accgcccgct tgagcagggc gccgccaccg cgcagggcca cgtcggcgat
     1321 cagcaggttg gtggcgggat tggggctcgg cgtagccgcg gcgattgcca gatcgggggc
     1381 ctggtccatg gcatcttcgg ctttgacgac ggctttggaa cgcttgcgca ggaatgccat
     1441 ggtcatcgga gccttatgcc agccgggtta tttcttccgg ccgaaatcga tggtgacgac
     1501 gttcgaccca tcctcggcaa cgccggtttc accgccgtgt tcctccccat cgggcgcatc
     1561 attctcggca tcgtcatgcg gttcgggggc gaggtcgttg accgtggcct ggaattgcag
     1621 gccgaaatcg accgccggat cgacgaaggc ggtgatcgcc gcaaagggga tcagcagctt
     1681 ggccgggacc tggttgaacg agaggccgac cgaaaacgaa tcctcgccca ccgacaggtc
     1741 ccagaacttg ttctggagca cgatcgtcat ctcgtcgggg aagcgttcgc gcagctgcgt
     1801 cgggatttcc acgcccggag cgcctgtctt gaaggtgatg tagaagtgat ggtggccagg
     1861 cagcatcccg cctccggctt ccacctggcc gagcacccgg ccaaccacgg cgcgcagggc
     1921 ttcctggacg atctcgtcgt aagggatcag actgtcgggc gtgtcgttca tcgtctctca
     1981 agtggcgacg gcggccgcga tggtcaagcg ccctgtcggt ccattgcagt ggagagaagc
     2041 ccaagacggc gaaaaatacg ttatcgtaca gccagttgcg tcaagccctc atcgcggccg
     2101 tagggatcgg agcggaaggc gaggcctcct gttttctgcg ggaagacggt caggtaggtg
     2161 atgtagaccg gcaccagctg gggcaggttc atgcgctttt ccggttcggc caccttgcgc
     2221 ggcagcggct tgcccatcag ccagcgccca agccgctgcg catcttccag ccgcacgcag
     2281 ccggaactgg cggtgcgctc atccttgcgc atcagggcct tctcgggcgt atcgtgaagg
     2341 tagatgccct gggggttggg gaattcgaac ttgacgcggc ccatgaaatt gctgccgccg
     2401 ggcagctggc gcacgacgac cgtggcgcgg ccgtcctcga ccgcctgcca gtcgaccagc
     2461 gacgggtcca tcggggcgga ggcatcccag tcgggcagga cctgataacc gccggtcttc
     2521 aggtaggtga ggcccttttc gagcacgttc caggcgatgc gctggcggac gaggtcctcg
     2581 ggcacgttcc agtaggggtt gaggatggca tagcggatga acccggccat catcggggtc
     2641 tggttgtcgg gcttgccgac gacgaccttc atcgaatcga ccgggcggcc gtgctcgaac
     2701 atccacagcc gcgccccggc ggcgtcgacc agcacgtagc gatccgccgg atagggcggc
     2761 agggcgcgca gccggtcgag gttgagggcg atctggtggc gctgctcgtc gtcatagtcg
     2821 tggctgagca gggcggcgcg cagcggggca taccacgggt gcatccaccc catctcggca
     2881 acgaactggt caagcgacgg cgctttcgcc gcggcggcga gcgcggcgga ctgggtgggc
     2941 accgccggtg caagggcttc gctctcgtat tgcatgtggg catggggggc ggagcgcagg
     3001 gccgtgaccc agttggcata gaccctcgag gcggcgaact cgacgcgggc gaggttggcc
     3061 ggctcgccat tgcccaagcg gtcgagcgcc ttgcgcaggt cgccagcgcg caggcgattc
     3121 tgcctgatcc cgtccatccg ggcctgttcg acctggccga gcaggaggaa ggcggcctgg
     3181 cggacccggc cggcctcgtc gagccacagc ggctgccagc cgcgcgcggc gtagaaggcg
     3241 cgcaggtcgc ccggtgcccg gtcggcgaga acggcgcgca tcgagggggc aactgcctgg
     3301 gcctgggcga gcgttggccc gggtgccccg ggcaggaggc ccagggccag agccagcgct
     3361 accggcgcca gtagccggcg gtagcgggga tgccgggcgg aggggctggt agcgctgctc
     3421 atcggatcag ccgcgctccc cggtgcgacg gtagttgtcg gtcggctgga tcacgggggc
     3481 gccttcgtag tagcggccct cgcgcacgta gccgtcgacg cagccgtcgt tgttgtgatc
     3541 ggcccagacg gcaccggcaa gtccgccgac cgccgcgcca aggaccgcgc cggcgaggac
     3601 attgccggcc agggccccga cagctgcgcc gccaaccccg ccgaccgccg cgccggttac
     3661 cgcggtgccg ccgaccgagc ggcaggcggg cgctgccgct gcggcagact ggtaattggg
     3721 atcatagccc tcgggcacgc ccggataata ctggccctcg cggacatagc cgtcgacgcg
     3781 gccgtcgcca tcgcggtccg cccagaccgc tccggcgatc ccgccgaccg ccgcgccgat
     3841 ggcggcgccg gccagcacgt cgccgccggc aagggcagcc ac
//
LOCUS       DATNBV010000081         3707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_60455,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000081 DATNBV010000000
VERSION     DATNBV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60455"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VN627_01910"
     CDS             <1..678
                     /locus_tag="VN627_01910"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD02041.1"
                     /translation="LVHADVREPEAMAAFFEQIDTVFGRVDVLVDVPGGGFVAPLMNT
                     TPKGWNAVMKQNFLYVLDTTQHAVRRMQAQKSGGSIIYITSIEAHRAVPTRAIYGAMK
                     AGVTNLAQSLALELGAEGIRVNTLAPDIFPTPATVSASEPVTEQEAALKAHIAIPMRR
                     YGEGPDLSGCALFLASDLSRYVTGTTLHCDGGTHASSGWFDWPDGGWNVTPSDGVARM
                     LAKGGAD"
     gene            815..1798
                     /locus_tag="VN627_01915"
     CDS             815..1798
                     /locus_tag="VN627_01915"
                     /inference="COORDINATES: protein motif:HMM:NF019174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HXD02042.1"
                     /translation="MRTKPAQSDADRLNALARIVLRPRPVSHKILLVLLGLAIAGGGR
                     WLFDQGAYGVPFLTFYPVVVLAALFLGADYALFASVAALLVIHFLLNTGSWNQAEGPV
                     RFAMLVVLSITLGVMITTGHFMRLVLFDNLRHLRQARAYNAELQHRAKNAQQMLRALI
                     GRGPAPGEDPAEFHAKLLGRVEALGRANELLGFGALEEASLADVVTSAIGPFDPGRFE
                     CAGPDGRLGKTAAIPLAMALHELGTNAIKYGALSAGGRVRVGWRQEGHGRYALEWREL
                     GGPAITVPTTHGMGSRLLRPHGGLARVELDWATAGLVCRMDLIGAEGDNGA"
     gene            1869..2225
                     /locus_tag="VN627_01920"
     CDS             1869..2225
                     /locus_tag="VN627_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXD02043.1"
                     /translation="MSNGSYEENIQLHRVRARRAPVALTCDIRQGQRPWAQSRLLNLS
                     ETGFCIVWLPALEHRRGLWLRIPGLQLLKANIRWRSGAMMGCEFEQRLHPAVFDHIVR
                     QANSGPGGLPAQMWRA"
     gene            complement(2264..3679)
                     /gene="lpdA"
                     /locus_tag="VN627_01925"
     CDS             complement(2264..3679)
                     /gene="lpdA"
                     /locus_tag="VN627_01925"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXD02044.1"
                     /translation="MADNYDVIVLGSGPGGYVAAIRAAQLGLKTAIVERENLGGICLN
                     WGCIPTKALLRSAEVFHQMKHASSYGLAAEGVTADLAAVVARSRGVAGQLNKGVTGLM
                     KKNKIAVHMGSGKLLGGGKLEVTGEKGAETLEAKHIIIATGARARDLPKAKADGKRIW
                     TYRHAMTPSELPSKLLVIGSGAIGIEFASFYNDMGSDVTVVEMMDRVVPVEDADVSAF
                     LEKALKKQGMKILTKASVGAIENGADAVTVTIKDKDGKETTEAYSHVIVAVGIVPNVE
                     NLGLEDVGVEPDKRYHIKIDGYGRTNVPGLWAIGDVVDGPWLAHKASHEGVTAAEAIA
                     QELGNKEVHPHPLDRSNIPGCTYCHPQIASVGLTEAKAKEAGLEVKVGMFPFIGNGKA
                     IALGEPEGFVKTVFDAKTGELLGAHMIGAEVTEMIQGYVVGRTLETTEAELMQSVFPH
                     PTISEAMHESVLAAYGRAIHI"
BASE COUNT          588 a         1227 c         1201 g          691 t
ORIGIN      
        1 ctggtccacg ccgacgtgcg cgagcccgag gccatggcgg cattcttcga gcagatcgac
       61 acggtgttcg gccgcgtgga cgtgctggtc gacgtgcccg gcggcggctt cgtcgccccg
      121 ctgatgaaca ccacgcccaa gggctggaac gcggtgatga agcagaactt cctctatgtg
      181 ctggatacca cccagcatgc cgtgcggcgg atgcaggcgc agaaatcggg cggttcgatc
      241 atctacatca cctcgatcga ggcccaccgc gccgtgccga cgcgggccat ctacggcgcg
      301 atgaaggccg gcgtcaccaa ccttgcccaa agcctcgcgc tcgaacttgg cgccgagggc
      361 atccgcgtca acaccctggc gccagatatc ttccccacac cggccaccgt gagcgcctcc
      421 gagcccgtca ccgaacagga agcggcgctc aaggcccaca tcgcaattcc catgcggcgc
      481 tacggcgaag gaccggacct ctcgggttgc gccctgttcc tcgcttccga tctgtcccgc
      541 tatgtcacgg gcacgaccct gcactgcgat ggcggcaccc atgcctcgtc cggctggttc
      601 gactggccgg atggcggctg gaacgtgacg ccatccgatg gcgtggcgcg catgctcgcc
      661 aagggtggcg ccgactgatc cgaggccctg ccggtaaaga tcttataaat attggtttgt
      721 tttaatgcaa ttgatgtaaa ttgtcttgat ctgaccaggc gccggtcaca atctgtcgcc
      781 gaacaggcat aaacgccgtc agggaaagga taatgtgagg acaaaaccgg cgcaaagcga
      841 tgctgaccgg ttgaatgcgc tggcgcggat cgtcctgcgt ccgcgcccgg tgagccacaa
      901 gatcctgctg gtgctgctcg gcctcgccat cgccggcggc gggcgctggc tgttcgacca
      961 gggggcttac ggcgttccgt tcctgacctt ctacccggtg gtcgtcctgg cagcgctgtt
     1021 ccttggcgcc gactatgccc tcttcgccag cgtggccgcc ttgctggtga tccacttcct
     1081 gctcaacacc ggcagctgga accaggccga ggggccggtc cgtttcgcga tgctggtggt
     1141 gctcagcatc acgctgggtg tgatgatcac caccggccac ttcatgcggc tcgtcctgtt
     1201 cgacaatctc cgacacttgc gccaggcccg cgcctacaac gccgagctgc agcaccgggc
     1261 caagaatgcc cagcagatgc tgcgcgccct gatcggccgc ggacccgcgc cgggggagga
     1321 tcccgccgaa ttccatgcca agctgctggg ccgggtggag gcgctggggc gggccaacga
     1381 gttgctcggc ttcggcgcgc ttgaggaagc gtcgcttgcc gatgtggtga ccagcgcgat
     1441 cggcccgttc gatccgggac ggttcgaatg tgccgggccg gacggccggc tgggcaagac
     1501 cgccgcgatc ccgctggcca tggccctgca tgaactgggg accaacgcca tcaagtatgg
     1561 cgcactgtcg gccgggggcc gcgttcgcgt cggctggcga caggagggcc atggccgcta
     1621 tgcgctggaa tggcgagagc ttggtggccc ggcgataacc gtgcctacca ctcatggcat
     1681 gggctcacgc ttgctgcgtc cgcacggagg gttggcccgc gtcgagctgg actgggcaac
     1741 ggccgggctc gtctgccgca tggacctgat tggcgccgaa ggcgataatg gcgcctgatc
     1801 agggaatacg gctccttaag gaagccgatg ctaccgcgtg aaacggacgg tttgtaacag
     1861 gcctcatagt gagcaacggg tcgtacgagg aaaacatcca gctgcaccgc gtgcgcgccc
     1921 gccgcgcgcc ggttgcgctc acgtgtgata tccgccaggg ccagcgcccg tgggcccagt
     1981 cgcgcctgct caacctttcc gaaaccggct tctgcatcgt ctggctgccg gcactggaac
     2041 atcgccgggg cctgtggctg cgcattcccg ggcttcagct gctcaaggcc aacatccgct
     2101 ggcgcagcgg cgcgatgatg ggctgcgagt tcgaacagcg gctccatccg gccgtgttcg
     2161 accacatcgt ccgccaggcc aacagtggcc ccggcggctt gcccgcgcag atgtggcgcg
     2221 cgtgatctgt tgaatgcggg aagcccggat gggcttcccg acttcagata tgaatcgccc
     2281 gcccgtaggc cgcgagcacg ctttcgtgca tggcctcgga gatcgtcggg tgggggaaca
     2341 ccgactgcat cagttcggcc tcggtggtct ccagcgtgcg gcccacgaca tagccctgga
     2401 tcatctcggt gacttccgcg ccgatcatgt gcgcgccgag cagttcgccg gtcttcgcat
     2461 cgaacaccgt cttcacgaaa ccttccggtt cgcccaacgc gatcgccttg ccgttgccga
     2521 tgaaggggaa catgccaacc ttgacctcga ggccggcctc cttggccttg gcctcggtca
     2581 ggccgacgct ggcgatctgc gggtggcaat aggtgcagcc ggggatgttg ctgcggtcca
     2641 gcgggtgcgg gtgcacctcc ttgttgccca gttcctgggc gatggcctcg gcggcggtca
     2701 cgccttcatg gctggccttg tgggcaagcc acggcccgtc gaccacgtcg ccgatcgccc
     2761 acaggcccgg gacattggtg cggccatagc cgtcgatctt gatgtggtag cgcttgtcgg
     2821 gctccacgcc gacgtcctcg agcccgaggt tctcgacatt gggaacgatg ccgacggcga
     2881 cgatcacgtg gctgtaggcc tcggtcgtct ccttgccgtc cttgtccttg atcgtgaccg
     2941 tcaccgcatc ggcgccgttc tcaattgcgc ccacgctggc cttggtgagg atcttcatcc
     3001 cctgcttctt gagcgccttc tcaaggaagg ccgaaacgtc ggcatcttct accggcacga
     3061 cccggtccat catctcgacg acggtaacgt cagagcccat gtcgttgtag aagcttgcga
     3121 attcgatgcc gatcgcgccc gagccgatga ccagcagctt actcggcagt tcagacgggg
     3181 tcatcgcatg gcggtaagtc cagatgcgct tgccgtccgc cttggccttg ggcaggtcgc
     3241 gggcgcgagc gccggtggcg atgatgatgt gcttggcttc gagcgtctcg gcgccctttt
     3301 cgccggtaac ttcgagcttg cccccgccga gcagcttgcc gctgcccatg tgcaccgcga
     3361 tcttgttctt cttcatcagc ccggtcacgc ccttgttgag ctggccggca accccgcgtg
     3421 agcgggcgac cacggcggcg agatcggcgg tgaccccttc ggcagccagg ccgtaggaag
     3481 aagcgtgctt catctggtgg aacacctcgg ccgagcgcag cagcgccttg gtcgggatgc
     3541 agccccagtt gaggcagatg ccgccaaggt tctcgcgctc gacgatcgcg gtcttgaggc
     3601 ccagctgcgc ggcgcggatc gcggccacat agccgccggg accggagccg aggacgatga
     3661 cgtcgtaatt atcagccatt gattgctcct aatccctctc cccttga
//
LOCUS       DATNBV010000082        10626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_691205,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000082 DATNBV010000000
VERSION     DATNBV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691205"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VN627_01930"
     CDS             <1..393
                     /locus_tag="VN627_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXD02045.1"
                     /translation="SLCSLIAGAQMHFAGRSLPEWNGRNYPWQAAQHAERGMKGRAEI
                     GHDRVIDSHYADLVRDPIGQMRKLYAQMGDELTPEAEAAMQAWLAENPQGRFGKHEYK
                     LEQYGTSKDKIAPMFEHYLSEYDIEMEG"
     gene            complement(433..1467)
                     /locus_tag="VN627_01935"
     CDS             complement(433..1467)
                     /locus_tag="VN627_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXD02046.1"
                     /translation="MSVRGHRITSFDVAALAGVSQSTVSRALAGDPVVSEPTRNRVVE
                     AARQLNYYVDENAARLRTGRTGTIAVVVISREAEDHADVNPFYFSLLGAICAAASNRG
                     HETIVSFQARPDSLAGRYEEQRKADGLIVIGTTANRPAWDYYRQLGEDGAHVCYWGSP
                     FDDLDWIRSDNHQGARLATGHLIDQGYRRIVCITSDHSPQRQFTERYVGYAERMREAG
                     LTPRDIQIDERLAREDQGRKAVTELIAGGDPFDAVFVCSDQIALGVLLELHERGIRVP
                     EQVGVAGFDGIRSGAYSIPPLTSLEPDFQSAGTMLVDKLLKQIAGQPSQERRVPVRLV
                     ERASSRGPAA"
     gene            complement(1535..2074)
                     /locus_tag="VN627_01940"
     CDS             complement(1535..2074)
                     /locus_tag="VN627_01940"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXD02047.1"
                     /translation="MTDQSQPPEQVVWSGKYITTKVRGTWEYVSRSRGIRAAVILAVD
                     EEDHVILVEQYRVPLGRSCIELPAGLIGDHDHLAGEDDHVAAARELEEETGYRAETME
                     VIGEFWSSPGMVSESYTLIRARGLVKVGAGGGTEGENITVHRVPLSALPAFVAGRRAL
                     GDAIDTKLLVVLAPHILGT"
     gene            complement(2075..2749)
                     /locus_tag="VN627_01945"
     CDS             complement(2075..2749)
                     /locus_tag="VN627_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXD02048.1"
                     /translation="MNPMTDADRARVAAAVTAAEAATSGEIVTVLAARSDDYADVALV
                     WSALVALAALLVVAWAPDFYLALYDRLTGAWVQHWTPGRLFGAAAFIAALKFGATWLI
                     LLWRPLRLALVPAPIRHARVHARALAAFRMGAERRTTGRTGILVYLSLAERRAEILAD
                     EAIASKVSADLWGEALAAMLAQLKQGRMADGLIAAVERVGAVLAEHLPRAADDSNELP
                     DRLIEV"
     gene            complement(2761..3576)
                     /locus_tag="VN627_01950"
     CDS             complement(2761..3576)
                     /locus_tag="VN627_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXD02049.1"
                     /translation="MPAPGRKLVLLWLTVLALLAPLAAHAEPTYPPLTGRVVDSANVI
                     PPDEEARLTQKLAALEAQSHRQLVVVTLPDLQGYDIADYGYQLGRHWGIGEKDKNTGA
                     LLIVAPNERKVRIEVGYGLEGTLTDGLSSLIIQQRIIPAFKAGDLPRGIEVGADAIIQ
                     QLTLPPDQAHKIAADANAQAAAPRSPGVDPGTVFFIIVFVLFFVLPALRRGFGGRRYS
                     SGGLGPLIVFDALSHMNRGGGSGWGGGGGGFGGGGGFSGGGGSFGGGGSSGSW"
     gene            complement(3582..4139)
                     /locus_tag="VN627_01955"
     CDS             complement(3582..4139)
                     /locus_tag="VN627_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXD02050.1"
                     /translation="MLSGCGINTVPTQEENAKARWADVQAAFQERANLIPNLAAVAKG
                     AAEQEKGILVGVVEARAKATSIQLSGDDLKDPAKMQAFQNAQNQLSTSVMGLGRLLAN
                     VEAYPELKSITNYQMLQSQLEGQENRIRVAIRDYNGAVQQYNTTIRTFPAMVAAKVIY
                     GAKPMVPYQAVTPGAEVAPSLEGKL"
     gene            4373..4789
                     /gene="mscL"
                     /locus_tag="VN627_01960"
     CDS             4373..4789
                     /gene="mscL"
                     /locus_tag="VN627_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXD02051.1"
                     /translation="MLSEFKAFIARGNVLDLAVGVIIGAAFGKIVSSLTDDVIMPVIS
                     TLTGGIDFSQKYVVLGTIPEGYKGEMTYAALKAAGVAMLGYGAFLTAVINFLILAFVI
                     FLIVRQANRMMPAKPAAPAGPSEVELLAEIRDALRK"
     gene            4935..5319
                     /gene="ssrA"
                     /locus_tag="VN627_01965"
     tmRNA           4935..5319
                     /gene="ssrA"
                     /locus_tag="VN627_01965"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            complement(5783..6028)
                     /locus_tag="VN627_01970"
     CDS             complement(5783..6028)
                     /locus_tag="VN627_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02052.1"
                     /translation="MLRKVGRLFVIKTRIEAFLIIYALALGATARGAHYLQQFPGWGG
                     KLLFLATTGAVFMAGAKILDCLKYEKADKERRDIEAF"
     gene            complement(6180..7022)
                     /gene="rsmA"
                     /locus_tag="VN627_01975"
     CDS             complement(6180..7022)
                     /gene="rsmA"
                     /locus_tag="VN627_01975"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXD02053.1"
                     /translation="MTPTLPPLREVIAAHGLSASKALGQNFLFDEQLLARIAAIPGNL
                     RGRPVLEVGPGPGGLTRALLRAGARVTAIEMDRRCLPALAELAEAFPGQLTVIEGDAL
                     KQDTAAHFSEPFAVVANLPYNVGTALFTGWLSGETWPPAWTSLTLMFQQEVAQRIVAD
                     PGTDAYGRLAVLAQWRSKPKLAMKVHRSAFTPPPKVMSAIVHVEPAAMPEGVSARMLE
                     RVTEAAFGQRRKMLRQSLKGLPGALAALDTLGIDPSRRAETLTIAEFVAVARTLSLQA
                     SSRA"
     gene            complement(7019..8023)
                     /gene="pdxA"
                     /locus_tag="VN627_01980"
     CDS             complement(7019..8023)
                     /gene="pdxA"
                     /locus_tag="VN627_01980"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXD02054.1"
                     /translation="MGMAPLAVSLGDPAGIGPELLATAWAAREVHHLPPFFAVGGAGL
                     LAAAASKRGLEVPIERISDPAQAAAVFAHALPVLGDEDGAYTPGHPTREGAALALASL
                     TRAAALAVSGAASGLVTGPIAKSRLAEVGFAHPGQTEFVAEASGTPPRDAVMMLAGPN
                     LRTVPLTVHVALAEVPRLVTRELIERKARIVAAALARDFGLADPCLAIAALNPHAGED
                     GRMGSEDAEIIAPAIAALRAEGIRATGPHPADALFAPHSRENFDVALCMYHDQALIPI
                     KALDFDAGVNVTLGLPIIRTSPDHGTAFAIAGKAIAHPGATIAAIRMAGECAGRRAGE
                     "
     gene            complement(8035..9297)
                     /locus_tag="VN627_01985"
     CDS             complement(8035..9297)
                     /locus_tag="VN627_01985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXD02055.1"
                     /translation="MEPGEAQGGLNIPDNIQIFGKNDPNHRRATAIVNGDIITGTDVE
                     QRLALVLSSSQAKPSEEEIARLRLQILRNLIDETLQIQEAKAQDLKVDDDQVEESYQR
                     VATQNFRQNPAALDAYLAKIGSSPASLKRQIRGELAWQAVLRRNVQPFVNVSESEVRE
                     SMDRLKAAKGTEEYRIGEIFLAATADTRPQVMANAKQIIEQIQKGGSFVAYARQYSQA
                     STAAVGGDLGWIRLAQLPTELASAAQGLQTGQLVGPIETSGGFSILYLIDKRQVLTAD
                     PRDAILSLKQVSIDFPEGTTEAQATKRAGEFAVAMKNAHGCGAVDPIAQSIGAQVVTN
                     DQVRARDLPAPLQDALLNLSLGETSPPFGSIKDGVRVLMLCGRDDPKIESGPSFDQMM
                     SQIEDDRVNKRAQAYLRDLRRDAVIEYN"
     gene            complement(9568..>10626)
                     /locus_tag="VN627_01990"
     CDS             complement(9568..>10626)
                     /locus_tag="VN627_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HXD02056.1"
                     /translation="DPVLGGKVELQLNTLAITRTAGQDTQRAFVKAQWDLTKVLPGGQ
                     LVSLTALARGDVYHSDQNSVLPKDLLYAGQTGWQGRGIAVGALDVKWPLVGSAFGGTQ
                     VFTPRVQLVASPPLRNLAVPNEDSRAIDLEDSNLFALNRFPGYDRVEDGVRFTYGFDW
                     QLDRPNWRIKTTLGQSYRLSNQATVVPTGTGLADRLSDIVGRTEVRFKDLVKLTWRYR
                     LDKDNLAVRRNEVDATVGSARTYAELGYLRLNRNITQVEDLQDREELRAAGRIALARN
                     WSVFGSAVVNLTKAAATPTAPASDGFQPIRTRMGVAYSDDCMELALTWRRDYITAGDA
                     GRGNTVQIYFALHNLGFR"
BASE COUNT         1642 a         3207 c         4025 g         1752 t
ORIGIN      
        1 tcgctgtgca gcctgatcgc gggcgcgcag atgcatttcg ccggacgcag cctgcccgag
       61 tggaatggca ggaactatcc gtggcaggcg gcgcagcatg ccgagcgcgg gatgaagggc
      121 cgggccgaga tcggccatga ccgggtgatc gattcccatt atgccgacct ggtgcgcgat
      181 ccgatcgggc agatgcgcaa gctctatgcg cagatgggcg acgagctgac ccccgaggcc
      241 gaggcggcaa tgcaggcctg gctggcggag aacccgcaag ggcggttcgg caagcacgag
      301 tacaagctcg agcaatatgg cacgagcaag gacaagatcg ccccgatgtt cgagcattac
      361 ctctcggaat acgacatcga gatggagggc tagggtcagg accccaggcg gcgaaaccct
      421 ggcagccgcc gctcaggcgg cggggccccg gctgctggcg cgttcgacca ggcggaccgg
      481 gacgcggcgt tcctggctcg gctggccggc aatctgcttg agcagcttgt cgaccagcat
      541 ggtgccggct gactggaaat cgggttcgag cgaggtcagc ggcgggatcg aataggcgcc
      601 ggagcggatg ccgtcgaacc cggccacgcc gacctgttcg ggcacgcgga tcccgcgttc
      661 gtgcagttcg agcaggacgc caagcgcgat ctggtcggag cagacgaaga cggcgtcgaa
      721 cggatcgccg ccggcgatca gctcggtcac ggccttgcgg ccctggtcct cgcgggcgag
      781 gcgctcatcg atctggatat cgcgcggggt gagcccggcc tcgcgcatgc gttcggcata
      841 gccgacatag cgttcggtga actggcgctg gggtgaatgg tcggacgtga tgcagacgat
      901 ccggcgatag ccctggtcga tcaggtgccc ggtggcgagg cgagcgccct ggtgattgtc
      961 ggaccggatc cagtccagat cgtcaaacgg ggagccccaa tagcagacgt gggcgccgtc
     1021 ttcgccgagc tggcgatagt agtcccaggc cgggcggttg gccgtggtgc cgatgacgat
     1081 caggccatcg gccttgcgct gctcctcata gcggccggcg aggctatcgg gccgggcctg
     1141 gaacgagacg atggtttcgt gcccgcgatt ggaggcggcg gcgcagatcg cgccgagcag
     1201 cgagaaatag aacgggttga cgtcggcatg gtcttccgct tcccggctga tcaccacgac
     1261 ggcgatcgtg ccggtgcgcc cggtgcgcag gcgggcggcg ttttcgtcga cgtagtagtt
     1321 cagctggcgg gcggcttcga ccacgcggtt gcgggtgggc tcgctgacca cgggatcgcc
     1381 ggcgagggcg cgcgacacgg ttgactggct gaccccggcc agtgccgcta catcgaatga
     1441 agtaatccgg tgcccgcgca cgctcattgc catcccgtta tgaccccggg cgggcttttc
     1501 ggcgcttgag gcccgaaagt aaagggttgc accttcaggt gccgaggatg tgcggggcga
     1561 ggaccaccag cagcttggtg tcgatggcat cgcccagcgc ccggcgccca gcgacgaagg
     1621 cgggcagggc ggaaaggggg acgcggtgga cggtgatgtt ctcgccttcg gtaccgccgc
     1681 cggcgccgac cttcacgagg ccgcgggcgc ggatcagggt gtagctctcg ctgaccatgc
     1741 cgggggagga ccagaattcg ccgatcacct ccatggtctc ggcgcggtag ccggtttcct
     1801 cttccagctc gcgcgcggcg gcgacgtgat cgtcctcgcc ggcgaggtga tcgtgatcgc
     1861 cgatgaggcc ggcggggagt tcgatgcagc tgcgccccag cggcacgcga tattgctcga
     1921 cgaggataac gtgatcctcc tcgtccaccg cgaggatcac ggcggcgcgg atgccgcgcg
     1981 agcgggagac gtattcccag gtgccgcgga ccttggtggt gatgtacttg cccgaccaga
     2041 cgacctgttc ggggggctgg gattgatcag tcactcacac ctcgatcagc cggtccggca
     2101 gttcgttgct atcgtccgcc gcgcggggga ggtgctcggc aaggaccgcg ccgactcgct
     2161 cgaccgcggc gatcaggcca tcggccatgc ggccctgctt gagctgggcc agcatggcgg
     2221 ccagcgcctc gccccatagg tcggcactga ccttgctggc aatggcttcg tccgccagga
     2281 tctcggcgcg gcgttcggcg aggctgaggt agaccaggat gccggtgcgc ccggtggtgc
     2341 gccgctccgc ccccatgcgg aaggcggcca gggcccgggc gtggacccgg gcgtggcgga
     2401 tcggggcggg gacgagggcc aggcgcagcg ggcgccagag caggatgagc caggttgccc
     2461 cgaacttgag cgcggcgatg aaggcggcgg cgccaaacag gcggccgggc gtccagtgct
     2521 ggacccaggc gccggtcagc cggtcataga gggcgaggta gaaatcgggg gcccaggcga
     2581 ccaccagcag cgcggcgagg gcgacgaggg ccgaccagac cagcgcgaca tcggcatagt
     2641 cgtcggaccg ggcggcgagg acggtgacga tctcgcccga ggtcgcggcc tcggcggcgg
     2701 tgaccgcggc ggcgacgcgg gcgcggtcgg catcggtcat ggggttcatg atggtatctc
     2761 ctaccacgag cccgacgagc cgccgccgcc gaagctgccg ccgccgcccg agaacccgcc
     2821 gccgcccccg aagccgcctc cgccgccgcc ccagccggag ccgccgccgc ggttcatgtg
     2881 gctgagcgcg tcgaacacga tgagcgggcc gaggccgccg ctcgaatagc gccggccgcc
     2941 gaagccgcgg cgcagggcgg gaaggacgaa gaacaggacg aaaacgatga tgaagaacac
     3001 ggtgcccgga tcgacgcccg gcgaacgggg cgcggcggcc tgggcgttgg cgtccgcggc
     3061 gatcttgtgc gcctggtccg gcggaagggt gagctgctgg atgatcgcat cggcgccgac
     3121 ctcgatcccg cgaggaaggt cgccggcctt gaaggcgggg atgatgcgct gctggatgat
     3181 gagggaggag aggccgtcgg tgagcgtgcc ttcgaggccg tagccgacct cgatccggac
     3241 cttgcgttcg ttgggggcga cgatcagcag ggcgccggtg ttcttgtcct tctcgccgat
     3301 gccccagtgg cggccgagct ggtagccata gtcggcaatg tcataaccct gcaggtcggg
     3361 cagggtgacg acgaccagct ggcggtggga ctgggcctcg agggcggcga gcttctgggt
     3421 gaggcgcgct tcctcgtcgg gcgggatgac attggcggaa tcgaccaccc ggccggtgag
     3481 cggggggtag gtgggttcgg catgagccgc cagcggcgcc agcagggcca gcaccgtgag
     3541 ccagaggagg acgagcttgc gcccgggagc gggcatggcg gtcagagctt gccttcgagg
     3601 cttggcgcca cttccgcgcc gggggtgacg gcctggtagg gcaccatcgg cttggccccg
     3661 tagatcacct tggcggcgac catggcgggg aaggtgcgga tggtggtgtt gtactgctgg
     3721 accgcgccgt tgtaatcacg gatcgccacg cggatgcggt tttcctggcc ctcgagctgg
     3781 ctctgcagca tctggtaatt ggtgatcgac ttgagctcgg gataggcctc gacgttcgcc
     3841 agcaggcggc cgagccccat caccgaggtt gagagctggt tctgcgcgtt ctggaaggcc
     3901 tgcatcttgg cggggtcctt caggtcgtcg cccgagagct ggatcgaggt ggccttggcc
     3961 cgcgcctcga ccacgccgac gaggatgccc ttctcctgct cggccgcgcc cttggcgaca
     4021 gcggcgagat tggggatgag gttggcgcgt tcctggaagg cggcctggac atcggcccac
     4081 ctggccttgg cgttttcctc ctgggtgggg accgtgttga tcccgcagcc cgagagcatg
     4141 aggctggcgg cgacgacggg cgcggcgaga gtcaggcgat tggcagacac ggtggttccc
     4201 tccactggct ccggcgcggc gccggtttgg ccgaacatag tgcacggggg gcaacgttca
     4261 aggcggggct ttgtgtcctg gcaaggccgt cgattgcgtg cgccaccatt tcggggcaca
     4321 acttggcccg agggtcggag tgctcagtaa gttgcagcag aggggatcga ctatgctttc
     4381 ggaattcaag gcttttatcg cgcgcggcaa tgtcctcgat ctcgctgtcg gcgtgatcat
     4441 cggcgcggcc ttcggcaaga tcgtctcgtc gctcaccgac gacgtgatca tgccggtcat
     4501 ctcgaccctg accggcggca tcgatttctc gcagaaatac gtggtgctgg gcacgatccc
     4561 ggagggctac aagggcgaga tgacctatgc cgcgctgaag gcggcgggcg tggccatgct
     4621 gggttatggc gctttcctga cggcggtgat caacttcctg atcctggcct tcgtgatctt
     4681 cctgatcgtg cgccaggcca accggatgat gccggccaag cccgctgcgc cggcggggcc
     4741 gagcgaggtg gaattgctgg cggagatcag ggacgccttg cggaagtagg cgaaaatcct
     4801 ccccgcacgc ggggaggtgg cagcccgtca gggctgacgg ggcggcttgc ggaccggcgc
     4861 tgcgtctggc cgagagcccc ctccaccgcc gttccggcgg tcccctcccc acgagtgggg
     4921 aggatttggg cccggtcctt ggcaaacggg gtcccgtccc ctatatccgc tttcgccggt
     4981 ttcgaccggc tatggtgata aattgcggcg tgcaataggc gcagcggacc cggggccggt
     5041 acccggcggc tccaccatcc tttccgtggc gacacgggaa agttgacggg gccgaactag
     5101 gatcgacgtg tgttgaaaag cgcagttttc acccgggctg agtaacccgt tcaaggctca
     5161 aaactcacaa gtgccaacga caacgacgca cttgctcttg ctgcgtaatt ctagggccta
     5221 acggcctgaa gttacaaagc cagaacacgg ttgaacccac cgggtaacag aagggaattc
     5281 agcggctggg ggcgagccgg gcaacagaat cgccccactt tcatgcccgt gagaggcccc
     5341 atgccgggct gcaaatcgcg cctttctgcg cttccggtgc tcacgtactg aagtacgctg
     5401 cgcgccggtt ctcgaaattc acgattttcg cctcggcctg gagcctctca cgggcatgaa
     5461 agccccaccc atttacctca gatttccgcc atgccgggct gcgaaccggc gctggctgcg
     5521 cttctggcgc tcacgtactg aagtacgctg cgcgccggtt ctcgccatca ccagtcctcg
     5581 ctccggcctg acgaaaatct gaggtaaatg agccctcctg ccatgcgccg tatctgcatg
     5641 catcgtcacc ctgaacttgt ttcagggtcc atggcgcttg ctggcagggt tgcggcaggc
     5701 tggcagacgt tgcttggttt gctcaggtgg gcccatggat gctgaaacga gttcagcatg
     5761 acgggtattc gcatctcggt ttttagaagg cctcgatgtc gcgccgttcc ttgtcggcct
     5821 tttcgtattt caggcagtcg aggatcttgg cgccggccat gaagaccgcg ccggtggtgg
     5881 cgaggaacag gagcttgccg ccccagccgg ggaactgctg gagatagtgc gcgccgcggg
     5941 ccgtggcgcc gagcgcaagg gcatagatga tgaggaacgc ctcgatccgg gtcttgatca
     6001 cgaagaggcg gccgaccttg cgcaacatgt cctgacccgg gtcccctgct tcatccgaat
     6061 tgccccggat gtggtttgca acgaatacgc aacgttcatg ccacacgccg cgttccgcgg
     6121 aaagtggtgg ttaacagttc ggcaagtgta aggctgggcg acgtcgcgcc ggtcccgggt
     6181 caagcccggg acgacgcttg taggcttaac gtccgggcca cggcgacgaa ttccgcgatg
     6241 gtcagggttt cggcgcggcg ggaggggtcg atgccgagcg tgtcgagcgc ggcgagggcg
     6301 ccggggaggc ccttgaggct ctggcgcagc atcttgcggc gctggccgaa ggcggcttcg
     6361 gtgacccgtt cgagcatgcg ggcggagacg ccttcgggca tggcggccgg ttcgacgtgg
     6421 acgattgcgc tcatcacctt gggcggcggg gtgaaggccg agcggtggac cttcatcgcc
     6481 agcttcggtt tgctgcgcca ctgcgcgaga acggcgaggc ggccgtaggc gtcggtgccg
     6541 gggtcggcga cgatgcgctg ggcgacttcc tgctggaaca tcagggtgag cgaggtccac
     6601 gcgggcggcc aggtttctcc cgagagccag ccggtgaaca gggccgtgcc gacgttgtag
     6661 ggcaggttgg cgaccacggc gaagggttcg gagaagtgcg cggccgtgtc ttgcttgagc
     6721 gcatcgcctt cgatcacggt gagctggccg gggaaggctt cggccagttc ggcgagggcg
     6781 ggcaggcagc ggcggtccat ctcgatggcg gtgacgcggg cgccggcgcg cagcagggcg
     6841 cgggtgaggc cgccgggacc cgggccgact tcgaggaccg ggcgaccgcg caggttgccg
     6901 gggatggcgg cgatgcgggc gaggagctgt tcgtcgaaca ggaagttctg gccgagtgcc
     6961 ttgctggccg agaggccatg ggcggcaatg acttcgcgca aggggggaag ggtgggcgtc
     7021 attcgccggc ccgccggccc gcgcattccc ccgccatgcg gatggcggcg atggtcgcgc
     7081 ccgggtgggc gatggccttg ccggcgatgg cgaaggcggt gccgtgatcg ggcgaggtgc
     7141 ggatgatcgg caggccaagg gtgacattga ccccggcatc gaaatcgagc gccttgatcg
     7201 ggatcagggc ctggtcgtgg tacatgcaca gggcgacgtc gaagttttcg cggctgtggg
     7261 gggcgaacag ggcgtcggcc gggtgggggc cggtggcgcg gatgccttcg gcacggaggg
     7321 cggcgatggc gggggcgatg atctcggcat cttcgctgcc catgcggccg tcctccccgg
     7381 catggggatt gagcgcggcg atggcgaggc acgggtcggc aagaccgaaa tcgcgggcga
     7441 gggcggcggc gacgatgcgg gccttgcgct cgatcagctc gcgcgtgacg aggcgcggga
     7501 cctcggcgag ggcgacgtgg acggtgagcg ggaccgtgcg caggttgggc ccggcgagca
     7561 tcatcaccgc gtcccggggg ggcgtgccgc tggcctcggc gacgaattcg gtctgccctg
     7621 ggtgagcgaa gccgacttcg gcaagacggc tcttggcgat cgggccggtg acgaggccgg
     7681 aggcagcacc ggagacggcg agcgcggcgg cgcgggtcaa cgaggcgagg gcgagggcgg
     7741 ccccttcgcg ggtggggtgg ccgggggtgt aggctccatc ctcgtcgccg agcacgggga
     7801 gggcatgggc aaagaccgcg gcggcctggg cggggtcgga gatacgctcg atcgggacct
     7861 cgagaccgcg cttgctggcg gcggcggcga ggaggcccgc gccgccgacc gcgaagaacg
     7921 gcgggaggtg atggacctcg cgcgcggccc aggccgtggc cagcagttcg gggccgatcc
     7981 cggcgggatc gccgagggag accgcgagcg gtgccatgcc catgggttgc ccgatcagtt
     8041 gtactcgatc accgcatcgc ggcgaaggtc gcgcaggtag gcctgggcgc gcttgttcac
     8101 gcggtcatcc tcgatctggc tcatcatctg gtcgaacgag gggccgcttt cgatcttggg
     8161 atcgtcgcgc ccgcagagca tcagcacgcg cacgccgtcc ttgatcgagc cgaacggagg
     8221 cgaggtttct ccgagtgaga ggttgagcag ggcatcctgc agcggggccg gcaggtcgcg
     8281 cgcgcggacc tggtcgttgg tgacgacctg ggcgccgatg gattgggcga tcggatcgac
     8341 cgcgccgcag ccgtgggcat tcttcatcgc gaccgcgaat tcgccggcgc gcttggtggc
     8401 ctgggcctcg gtcgtcccct caggaaagtc gatcgagacc tgcttgagcg agaggatcgc
     8461 atcgcgcgga tcggcggtga ggacctggcg tttgtcgatc aggtagagga tcgagaagcc
     8521 gccgctggtt tcgatcgggc cgaccagctg accggtctgc aggccctggg cggccgaggc
     8581 cagttcggtg ggcagctggg cgagacggat ccagccgaga tcgccgccga ccgccgcggt
     8641 ggaagcctgc gaatactggc gggcataggc gacgaagctg ccgcccttct ggatctgctc
     8701 gatgatctgc ttggcattgg ccatgacctg cgggcgggta tcggccgtgg cggcgaggaa
     8761 gatttcgccg atgcggtatt cctcggtgcc cttggcggcc ttgagccggt ccatggattc
     8821 gcgcacttcg ctttccgaga cgttgacgaa gggctggacg ttgcggcgca ggaccgcctg
     8881 ccaggcgagc tcgccccgga tctggcgctt gagcgaggcc ggcgaggagc cgatcttggc
     8941 gaggtaagcg tcgagcgccg cgggattctg gcggaagttc tgcgtggcga cgcgctggta
     9001 ggattcttcc acctggtcat catcgacctt gaggtcttgt gccttggctt cctggatctg
     9061 cagcgtctcg tcgatcaggt tgcgcaggat ctgcaggcgc aggcgggcga tttcctcttc
     9121 ggagggcttg gcctggctgg acgagaggac cagcgcgagg cgctgctcca catcggtccc
     9181 agtgatgatg tccccgttga cgatggccgt ggcgcggcgg tggttgggat cgttcttgcc
     9241 gaagatctgg atgttgtcgg gaatgttcag gccgccctgg gcttcgccgg gctccatcgt
     9301 ctgggcagcg gtcacgctgg cgagcgcggc ggcggacagg ccgagcacga tccggcgggc
     9361 aatggaaaag cgcggagaag tcttcacctt ggcatcgttc ctgtctgcgg gggaaacccc
     9421 gagcccccga taggcccgaa ctgcggcgat ccggcttaac cggacctgag catgggcgca
     9481 cgcgcggtcg atagcggttt tacggttggg cgcaaacagg aagtggctgt gaggcgtgcc
     9541 gcggccgggg attaccgccc gtgcgcctca gcggaagccg agattgtgca gggcaaagta
     9601 gatctggacg gtgttgccgc ggccggcatc gcccgcggtg atatagtcgc gccgccaggt
     9661 gagggcgagt tccatgcaat cgtcggaata ggcgaccccc atgcgggtgc ggatcggctg
     9721 gaagccgtcg gacgcggggg cggtgggcgt ggcggcggcc ttggtcaggt tgaccacggc
     9781 cgagccgaac accgaccagt tgcgggccag ggcgatccgg ccggcggcgc gcagctcctc
     9841 gcggtcctgc aggtcttcca cctgggtgat gttgcggttg aggcgcaggt agccgagctc
     9901 ggcgtaggtg cgggccgagc cgacagtggc gtcgacctcg ttgcggcgga cggcgaggtt
     9961 gtccttgtcg agccggtagc gccaggtcag cttgaccagg tccttgaagc ggacctcggt
    10021 gcggccgacg atgtcggaca ggcgatcggc gaggccggtg ccggtgggca cgaccgtggc
    10081 ctggttggaa agccggtagg actggccgag cgtggtcttg atccgccagt tgggccgatc
    10141 gagctgccag tcgaacccgt aggtgaagcg caccccgtcc tccacccggt cgtagccggg
    10201 gaagcggttg agcgcgaaga ggttggaatc ctcaaggtcg atcgcccgcg aatcctcgtt
    10261 ggggacggcg aggttgcgca gcggcgggct ggcgacgagc tggacgcgcg gggtgaacac
    10321 ctgggtgccg ccgaaggcgc tgccgaccag cggccacttc acatcgagcg cgccgacggc
    10381 gatgccccgg ccctgccagc cagtctgccc ggcataaagc aggtccttgg gcagaacgct
    10441 gttctggtca gagtgataga catcgccgcg ggcgagtgcg gtcaggctga cgagctggcc
    10501 cccggggagc actttcgtca ggtcccactg ggccttgacg aaggcgcgct gggtatcctg
    10561 cccggcggtg cgggtgatcg cgagggtgtt gagctgcagc tcgaccttgc cgccgaggac
    10621 cggatc
//
LOCUS       DATNBV010000083         2294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1642045,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000083 DATNBV010000000
VERSION     DATNBV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642045"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VN627_01995"
     CDS             <1..570
                     /locus_tag="VN627_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXD02057.1"
                     /translation="LHDKVLHNRYDAAVPTGFARARAVFLLREPVPTIRSIRHLFRTI
                     GSSEYANDALAADYYAERLDQLARLWRLFPAQRRIGIDFAELTGGPEAVLARLSGFLG
                     LQTPLVNAYGPAGPERHGLGDPLTSPRFSAIVGPAKPAPGQSDDGLPALPQRRIAELE
                     LAHYRVRQLICGDPPGSGDQRAKDSSQSR"
     gene            774..>2294
                     /locus_tag="VN627_02000"
     CDS             774..>2294
                     /locus_tag="VN627_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HXD02058.1"
                     /translation="MNQFLGQEPGKPGWFGLARSGELLRALPGGRQVIGMIMGMSAAL
                     NVLLLSGSIFMLMVYDQVLPSHSVPSLIGLVVLLIVAFGFQAALDHLRHRISGAAGEM
                     LERSLSDRVFGLVLESELRGGREAGQPVRDLDLLRSFLASPAPMAVLDLPWMLLFMGV
                     LFAFHWILGLVCLVGAVLVVGLTLWTDRMTTAQIQEGTRSGSERAGFLEACRRNAEVI
                     RALGMRGQIERGWERLSETHAEVHDAAGERLSAMRTFSKTFRQLLQSLILATGAWLVI
                     DGQASGGVIIASSILSSRALGPIDSAIAHWRTLISARQAWNRLALRLSASPEALARTE
                     LPRPSKTVTVESLSAVVPGTQTLLFRDVSFNLAAGESLAVVGASGSGKSSLVRALVGV
                     LPPIRGAVRLDGAALDQWDPDLAGQFIGYLPQDVELFDGTVAQNIARFDPEASAEEVV
                     AAATRAGVHDLIVHMPQGYDTVLGPGGRNLSAGQRQRIALARALFRDPFIVVLDEPN"
BASE COUNT          328 a          811 c          731 g          424 t
ORIGIN      
        1 cttcacgaca aggttctgca caaccgctat gatgccgccg ttccgaccgg tttcgccagg
       61 gcccgggccg tcttcctcct gcgcgaaccc gtgcctacga tccgctcgat caggcacctg
      121 ttccgcacca tcggcagcag cgaatatgcg aacgatgccc tcgccgccga ttactatgcg
      181 gagcggctcg atcagctcgc gcggctatgg cgcttgttcc cggcacagcg gcgcatcggc
      241 atcgattttg ccgagctcac cggcgggccc gaggccgtcc tggcccgcct gtcgggattt
      301 ctcggcctcc aaaccccgct ggtcaacgcc tatggccccg ccgggcccga gcgccatggc
      361 ctgggtgacc cgctcacttc gccccggttc tcggcgatcg ttggtccggc caagccggcg
      421 cccggccaat cggacgatgg tctccctgcc cttccgcaac ggcggatcgc cgaactcgag
      481 cttgctcatt accgggttcg gcaactgatc tgcggtgatc cccccggctc aggtgaccag
      541 cgcgccaagg acagttccca atcgcggtga aaattgccca tcctttccaa gcacctggcc
      601 cattattccc taaccctgtg aagatgcgca gaaatatcct ggtagcgttt aggtccgttg
      661 aggatactaa gggcctcccg gcagcgagac taatttgcta atgggccggg gtcgggcact
      721 tatttgatgt cgccggcaga gtagttcgag agtgcaggcg ggggctagcg ggaatgaacc
      781 agtttcttgg gcaggaaccc ggcaagcccg gctggttcgg gcttgcccgt tcgggtgaac
      841 tgctgcgggc gctgccgggc ggacggcagg tcatcggcat gatcatgggg atgagcgcgg
      901 cgctgaacgt gctgctgctc tccggctcga tcttcatgct gatggtctat gatcaggtcc
      961 tgcccagcca ctcggtaccc agcctgatcg gcctcgtggt gctgctgatc gtcgccttcg
     1021 gcttccaggc cgcactcgat cacctgcgcc accgcatctc gggcgccgcc ggcgaaatgc
     1081 tggagcgcag cctgtctgac cgggtgttcg gcctggtcct cgaatccgaa ctgcgcggag
     1141 gccgcgaagc cgggcagccg gtgcgcgacc ttgatctgct gcgctcgttt ctcgccagcc
     1201 ccgcgccgat ggccgtgctg gatctcccgt ggatgctgct gttcatgggc gtgctgttcg
     1261 ctttccactg gatcctcggg ctggtctgcc tggtcggcgc ggtgctggtg gtcggcctga
     1321 cgctatggac cgaccggatg accacggcgc agatccagga gggcacccgc agcgggtcgg
     1381 agcgcgccgg cttcctcgaa gcctgccgcc gcaacgcgga ggtgatccgc gccctcggca
     1441 tgcgtggcca gatcgaacgc gggtgggaac ggctgagcga aacccacgcc gaagtgcacg
     1501 atgcggcggg cgaacgcctc tccgccatgc gcaccttctc gaagaccttc cgccagctgc
     1561 tccagtcgtt gatcctcgcc accggcgcct ggctggttat tgatggtcag gcttcgggag
     1621 gggtgatcat cgccagctcg atcctctcct cgcgcgcgct cgggccgatc gacagcgcga
     1681 tcgcccattg gcgcaccctg atttccgcgc gccaggcttg gaaccgcctc gccctgcgcc
     1741 tttccgccag ccccgaagcc ttggcgcgga ccgagctgcc ccgcccgagc aagaccgtca
     1801 cggtcgaatc gctctctgcc gtcgtccccg gcacgcagac gctgctgttc cgcgacgtca
     1861 gcttcaatct tgccgcgggg gaaagcctgg ccgtggtcgg cgccagcggc tccggcaaga
     1921 gctcgctggt gcgggcgctg gtcggcgtac tcccccctat ccgcggggcc gtccgccttg
     1981 acggcgcggc gctcgatcag tgggatcccg atcttgccgg ccagttcatc ggctacctgc
     2041 cgcaggacgt cgaactgttc gatggcacgg tcgcccagaa catcgcccgc ttcgaccccg
     2101 aggccagcgc cgaggaagtg gtcgccgccg ccacccgcgc gggcgtgcac gatctcatcg
     2161 tccacatgcc gcagggctac gataccgtgc tcggccccgg cgggcgcaac ctctcggcgg
     2221 gccagcgcca gcgcatcgct ctcgcccgcg ccctgttccg cgatccattc atcgtcgtgc
     2281 tggatgagcc caat
//
LOCUS       DATNBV010000084         2589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_62989,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000084 DATNBV010000000
VERSION     DATNBV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62989"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VN627_02005"
     CDS             <1..492
                     /locus_tag="VN627_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02059.1"
                     /translation="YLLALIGLALAWAGLVWPALLLLGIACVSQRAAALLGHLQREAL
                     AARAGPPWRSAVSGIAVDVAIAVVLILAQPALPGESLLQRCFAPVVLIGLVRLLPRVF
                     NAGWTTWLADRLLLCLLLALMAVGGVIGSAVPAFALLLLLAGLVLNHAASGAGRSSGL
                     TRA"
     gene            612..1397
                     /locus_tag="VN627_02010"
     CDS             612..1397
                     /locus_tag="VN627_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02060.1"
                     /translation="MLPRLVDDDPAAISEDLVIQTRAMIAALAADLLRDDPETVRVVA
                     LERSLISRPGLLAHCHCLVLEAGSAERLSASGFDPVLSPVFEALLAADAETAHLAMSA
                     LAAQSRFVTRHRRLETTAAELPAELMHEALVALADLAGDDLAVRWRAGFDEGRSRLSL
                     LARLALAAGEQEQRLLDPAAAGLALFSTALAQRAGVARDEAILAGAPGQQLRLALLLR
                     AAGVAPDAARAAVGLIHGDAPLPDNWDELTGARAAALLAGADA"
     gene            1394..>2589
                     /locus_tag="VN627_02015"
     CDS             1394..>2589
                     /locus_tag="VN627_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXD02061.1"
                     /translation="MTGSETILAIGRTDAENQLTQADELLLGLQLRCGGELAGPVAVP
                     ALREAILRTRATGLRLALPITVHDGVELVTAWVELTPLVGTDDGCAIAVRQWHARPLQ
                     QDDEAVAARRRALLARQAAEFSARLDARQCALVAEGTAPDLAGLGERMTAEPGKAWTE
                     FVTVEGSAHRQPLHWRLLDGARVRVAGSDRLWQVALLPLGDMAGGPAGFELHLTSDVP
                     FPVAAEPNGSSKGAPERMIGREVAPVLRQPIARIVANAETIRSRLMGPLADEYSAYAA
                     DIAAAGQHLLALIDDLTDLEVVESAEFTTSPDRIDLAEVARQAAGILGVKSRERGIEI
                     DAPKLGESLGAVAEFRRVLQVLLNLIGNALRYGPEGSQVWIRLEHDGQFSRVIVADQG
                     PGLDAA"
BASE COUNT          355 a          824 c          922 g          488 t
ORIGIN      
        1 tacctgctgg ccctcatcgg tttggcgctg gcttgggcgg gtctggtctg gccggcgctg
       61 ctgttgctgg gcatcgcctg tgtgtcccag cgcgctgcgg ctctgctcgg gcacttgcag
      121 cgcgaggcgc ttgcagcgcg agcggggccg ccctggcgta gcgcggtttc agggattgca
      181 gtggacgtgg cgattgctgt cgtcctaatc ctcgcccaac cagcgctgcc gggcgaatcc
      241 ttgctccagc ggtgtttcgc tcccgtggtt ctgattgggc ttgtccggct gctgccgcgt
      301 gtgttcaatg ccggctggac gacgtggctg gctgaccggt tgttgttgtg cctcctgctc
      361 gccctcatgg ctgtcggggg cgtaatcggc tcggccgtgc cggcctttgc gctcctgctg
      421 ctgctggctg gactggtgct caatcatgcc gcttcagggg cggggcggag cagcgggcta
      481 acgcgagctt aaccgcccgc agctatgggg tggcaatgac tatagcggag cagacaggcc
      541 aagccgccgc caatgccgca gtccttgcgg aatttgcccg cgaggagcgc gcgcgtctgg
      601 cgctggcaaa cgtgctgccg cgcctcgtgg acgatgaccc ggctgccatc agtgaagacc
      661 tggtgattca aacacgcgcg atgatcgccg cgctcgccgc cgacttgctc cgcgatgatc
      721 cggagacggt gcgcgtggtt gcgcttgagc gttcgctgat cagccgcccg ggcctgttgg
      781 cccattgcca ttgcctggtc ctggaagcgg gcagcgccga gcggctgtcg gcaagcgggt
      841 ttgaccccgt gctatcaccc gtgttcgaag ctttgctcgc cgccgatgcc gagaccgccc
      901 atctggccat gagtgcactt gccgcgcagt cgcggttcgt gacgcggcac aggcggctgg
      961 aaacgacggc cgcggaattg ccggcggagc tgatgcacga ggcgctggtg gcgctggccg
     1021 accttgccgg cgacgatctc gccgttcgct ggcgtgccgg gttcgatgag gggcgcagcc
     1081 gcctctcgct gcttgcccgt cttgccctcg cggcaggaga gcaggagcag cgcctgctcg
     1141 atcccgcggc agccggccta gctttgtttt ccacggcgct cgcccagcgt gccggagtgg
     1201 ctcgtgacga ggccatcctg gcgggagcgc caggtcagca gttgcgtctg gccttgctgc
     1261 tgcgggcagc cggggtggct ccggatgcgg ctcgtgctgc ggttggcctg atccatggcg
     1321 atgccccctt accggataac tgggacgaac tgaccggagc tcgagccgcc gctttgctgg
     1381 ccggggccga cgcatgaccg ggagcgagac tatcctggcc atcgggcgca ccgatgccga
     1441 gaaccagctg acgcaggccg acgagttgct gctcgggttg caactgcgct gtggtggcga
     1501 actggccggc ccggttgcgg tgcctgcgct gcgggaggca atcttgcgca cccgcgccac
     1561 cgggctgagg ctcgccctgc cgattaccgt tcacgatggg gtggaactgg taaccgcgtg
     1621 ggttgaattg accccattgg tcggcacgga cgacggctgc gccattgctg tgcggcagtg
     1681 gcatgcccgg ccgctgcaac aggacgacga ggcggttgcc gcccgccgtc gggcgctgct
     1741 cgccaggcaa gcagctgaat tttccgccag gctcgacgcg cgccagtgcg ccttggttgc
     1801 ggaaggcacc gcgccggacc tcgcgggtct gggagagcgg atgactgctg agccaggcaa
     1861 ggcctggact gagttcgtca cggtggaagg cagtgcccac cgtcagccgc tgcactggcg
     1921 gttgctcgac ggggcaaggg ttcgggtcgc cggttcggat cgcctatggc aggtcgccct
     1981 gctgccgctc ggcgacatgg ccggtgggcc cgccggattc gagctgcacc tgacgtctga
     2041 cgtaccgttt cccgttgctg cggaacccaa tggaagcagc aagggagcgc ccgagcgcat
     2101 gatcggccgc gaagttgcgc cagtgctgcg ccagccgatc gcgcgaatcg tggcgaatgc
     2161 ggagaccatc cgctctcgcc tcatgggccc acttgccgat gaatacagcg cctatgccgc
     2221 agacattgcc gccgccgggc agcacctcct ggccctgatc gacgatctca ccgatctcga
     2281 agtagtcgaa tctgccgagt tcaccacttc gccggacagg atcgatcttg ccgaggtcgc
     2341 ccgccaggct gccggtattc tgggggtcaa gtctcgcgaa cgggggatcg agatcgacgc
     2401 gcccaaactt ggcgaaagtc tcggggctgt cgcggaattt cgccgggtgc tccaggtcct
     2461 gcttaacctc atcggcaatg ccttgcgcta tgggccggag ggatcgcagg tgtggatccg
     2521 gttggaacat gacgggcagt tttcgcgggt gattgttgcc gatcaggggc cggggcttga
     2581 tgcggccca
//
LOCUS       DATNBV010000085         5872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1642391,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000085 DATNBV010000000
VERSION     DATNBV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642391"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="VN627_02020"
     CDS             <1..112
                     /locus_tag="VN627_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXD02062.1"
                     /translation="SHLCAANYCQRYRPAARHPEPVDTSTSHVGFRCVQR"
     gene            complement(171..1445)
                     /locus_tag="VN627_02025"
     CDS             complement(171..1445)
                     /locus_tag="VN627_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HXD02063.1"
                     /translation="MAIQAARAGAEQGGESIEAPQLRLFVMALFFIFGGITSLNDVII
                     PKLKELFTLNYTQAMLVQFCFFTAYAVIGIPGARLVKKLGYMRGAVAGLLTMLAGCLL
                     FIPASRTATYGLFLLALFVLASGVVVVQVVANPLISMLGKPETAHSRLTFAQAFNSLG
                     TTVFPYVGSLLILGGLAKVSASQLSGEALDAYRTSESQAIVHGYLGLAAALAVVAGVV
                     WLFRNALKGERHEESSGLAGLDLLKRPRFGLGALCIFLYVGAEVSIGSLIVNYLRQAS
                     VMGLEEQAAGKMIPLYWGGAMIGRFIGSAVLRVVSPGKVLAGVAAGAIALICISAATT
                     GPVSGYSLLAIGLMNAIMFPTIFSLACEKLGPRAADGSGIINVAICGGAVVPLMTGMT
                     ADATGSLAIGLFLPAICYGVIAYFGWYARRPA"
     gene            1680..3113
                     /gene="tldD"
                     /locus_tag="VN627_02030"
     CDS             1680..3113
                     /gene="tldD"
                     /locus_tag="VN627_02030"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HXD02064.1"
                     /translation="MTSPLVADPRSLLYRSGQLSPDEAQALAQETLKACDDGELYLQF
                     IASESFGFDDGRLKTADYSRDAGFGLRGISGEMTGFAHANEISAAAIRRAGETLKLLD
                     PATGQRAAPPQATNRHLYTDASPLDAVDFAAKVQLLETIDAVARARDPRVVQVSASLA
                     ASWSVVEIVRRDGFTATDVRPLVRLNVSIVAEQNGRRETGSFGMGGRRLYDDLFQPET
                     WNRAIDVALNQALTNLESVDAPAGEMTVLLGPGWPGVLLHEAVGHGLEGDFNRKGTSA
                     FSGRIGERVGAPGVTVVDDGSILGRRGSLSIDDEGTPTQETVLIEDGVLKGYIQDRLN
                     ARLMGVAPTGNGRRESYAHAPMPRMTNTFMRGGNDDPAELLGRVKNGIYAKAFGGGQV
                     DIVSGKFVFSCTEAYRIENGKLGAPIKGATLIGDGPSVLTRVTGIGNDMALDEGVGVC
                     GKGGQSVPAGVGQPTLLVEGLTVGGTA"
     gene            3137..3703
                     /locus_tag="VN627_02035"
     CDS             3137..3703
                     /locus_tag="VN627_02035"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXD02065.1"
                     /translation="MKPAFLIAAAAAVLAGLAAPVALSAAEPQPAAVAAAPAPRPYPQ
                     LTHVAWVVPNLDKALADGAAQLGTKPANLVFRRVLTVDRGTYQGRPAHFSAEFALLEL
                     GNTQFEFIQPLTGTSPYSDALKGHRGAVLHHLAFTVPNLDGQIAAARRANPQARVVLD
                     AHLKGFDARYVYMSGVMPGTLVEFIPGG"
     gene            3792..4196
                     /locus_tag="VN627_02040"
     CDS             3792..4196
                     /locus_tag="VN627_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02066.1"
                     /translation="MNKAIVLLAAGASLLAVPVMAAPRLTGEARLADILKGRVPGKPV
                     DCIDPRINSDTQIVDGTAIVYGSGRTVYVQRPDNARSLRSDDMLVTTLRGSSQLCSID
                     VVHLHDRSGGWNRGFVGLNKFVPYTREHVARN"
     gene            4302..4805
                     /locus_tag="VN627_02045"
     CDS             4302..4805
                     /locus_tag="VN627_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXD02067.1"
                     /translation="MQGEAKRDPRRATFLKVHVRSDNGWSEAVLCNVSAHGMMLRGDD
                     LPRRGNFIEIDSGTVTVVGQVRWALAGRCGIRTREAVDLGALLGDHPQGERHSAPAGP
                     VVRYAAGPRSRPATDSKAMARALDFGLTVALLAGGAWLLGTTVTGLFGTPLEQVKAEL
                     APHRPAG"
     gene            complement(4862..5647)
                     /locus_tag="VN627_02050"
     CDS             complement(4862..5647)
                     /locus_tag="VN627_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD02068.1"
                     /translation="MFDKLFAGKVALVTGSTSGVGRASARMLAQWGCAEVVINSRNPA
                     EGAQVRDALAAELPGTQFHFVRADMNSLEEIEALFAETGRLCGGLDVFVHSGYGGGGS
                     KPDLFENMTPGQSAEVIMGVFLSLVRCCHFAIPMLKARGGGAVIGVTSDAAKVPTPGE
                     AVHGGALAGSVMFLRGLAREMGRYGIRLNAVTPSLIKDTRNYDKVMDDGYSKKLFEKI
                     EKRAQLGLPGPDDLAALITFLASPLAAHITGQAVSCNGGISGA"
BASE COUNT          967 a         2152 c         1853 g          900 t
ORIGIN      
        1 ctcgcacctg tgcgcggcga actactgcca gcgctaccgc cccgccgcgc ggcatcccga
       61 accggtcgac acctcgacca gccacgtcgg cttccgctgc gtccagcgct gaccgggcca
      121 cgacccgccg gcggaaccgg gggaggccgc agcgctcccc cgccgtgcgc tcaggccggc
      181 cgccgcgcgt accagccgaa ataggcgatc acgccgtagc agatcgccgg aaggaacagg
      241 ccgatggcca ggctgcccgt ggcatctgcg gtcatgccgg tcatgagcgg caccaccgcg
      301 ccgccgcaga tcgctacgtt gatgatcccc gagccgtcgg ccgcgcgcgg gcccagcttc
      361 tcgcaggcga gggagaagat cgtcgggaac atgatcgcgt tcatcaggcc gatcgccagg
      421 aggctgtagc ccgagaccgg cccggtcgtc gccgccgaga tgcagatcag cgcgatcgcc
      481 ccggccgcca ccccggccag caccttgccc gggctcacca cccgcagcac ggccgagccg
      541 atgaaccgcc cgatcatcgc cccgccccag tagagcggga tcatcttgcc ggccgcctgc
      601 tcctccagcc ccatgaccga ggcctggcgc aggtagttca cgatcagcga gccgatcgag
      661 acttccgcgc cgacatagag aaagatgcag agcgccccca gcccgaagcg ggggcgcttc
      721 agcaggtcga gcccggcaag gcccgagctt tcctcgtggc gttcgccctt cagcgcattg
      781 cggaacagcc agaccacgcc cgcgacgacc gccagcgccg ccgcgaggcc aaggtagccg
      841 tggacgatcg cctggctttc ggacgtgcgg taggcatcca gcgcctcgcc cgacagctgg
      901 ctggcgctga ctttcgccag tccaccgagg atcaggagcg atccgacata ggggaagacc
      961 gtggtgccca gcgaattgaa ggcctgggcg aaagtcagcc ggctgtgggc cgtctccggc
     1021 ttgccgagca tcgagatcag cgggttggcc accacctgga ccaccaccac cccgctcgcc
     1081 agcacgaaca gcgccagcag gaacaggcca tacgtcgccg tccgcgaggc ggggatgaac
     1141 agcaggcagc cggcgagcat cgtcagcagg ccggcgacgg ccccgcgcat gtagcccagc
     1201 ttcttgacca gccgcgcacc gggaatgccg atcaccgcat aggcggtgaa gaagcagaac
     1261 tgcaccagca tggcttgggt gtagttgagc gtgaacagct ctttcagctt ggggatgatc
     1321 acgtcgttga gcgaggtgat cccgccgaag atgaagaaca acgccatgac gaacagccgc
     1381 agctgcggcg cctcgatgct ttcgccgccc tgttcggccc ctgcacgggc cgcctggatc
     1441 gccattcttc ctctcccttg ctcctgatgc ggttcaccgc gcggatgctc gaataaattt
     1501 cgcatgtcgc aataccggga cattgcatcc atgcaaatgc gcagcgaacg acctgccttg
     1561 cccatcacta tgggaacatg cggtttgcct caacgcgttt tgcagcgctg gggcgatttg
     1621 accgccctca tgctccgggc ccgctggcca ttgcccgctg cgctccctat atccgggcga
     1681 tgacttctcc cctcgtcgcc gatccgcgct cgctgcttta ccgttccggc cagctttccc
     1741 ctgatgaggc ccaggccctc gcgcaggaaa cgctcaaagc ctgcgacgat ggcgagctct
     1801 atctccagtt catcgccagc gaatcctttg ggttcgatga tggccgcctg aaaacggctg
     1861 actattcacg cgatgcgggc ttcggcctgc gcggcatttc cggcgagatg accggatttg
     1921 cccatgccaa cgagatcagc gccgccgcca tccgccgcgc cggcgagacg ctcaagctgc
     1981 tcgatcccgc cacgggccag cgcgccgcgc cgccccaggc caccaaccgg cacctctata
     2041 ccgatgcctc tccgctcgac gcggtggatt tcgccgccaa ggtccagctg ctggaaacca
     2101 ttgatgccgt ggcccgcgcc cgcgatccac gggtggtgca ggtctcggcc agcctggccg
     2161 cctcctggtc ggtggtcgag atcgtccggc gcgacggctt caccgccacc gacgtgcgcc
     2221 cactggtccg gctcaacgtc tcgattgtcg ccgagcagaa cggccggcgt gagacaggga
     2281 gcttcggcat gggcggccgc cgcctctatg acgacctgtt ccagcccgaa acctggaacc
     2341 gcgcgatcga cgtggccctg aaccaggcgc tgaccaatct tgaatcggtc gatgccccgg
     2401 ccggcgagat gaccgtgctg ctcgggccgg gctggcccgg cgtgctgctg cacgaagccg
     2461 tgggccatgg cctggaaggc gatttcaacc gcaagggcac cagcgccttc tccggccgca
     2521 tcggcgaacg cgtcggcgct ccgggcgtga ccgtggtcga cgatggctcg atcctcggcc
     2581 ggcgcggctc gctgtcgatc gacgacgagg gcacgcccac ccaggaaacc gtgctgatcg
     2641 aggacggcgt gctcaagggc tatatccagg accgcctcaa cgcccgcctg atgggggtcg
     2701 cgcccaccgg caacggacgg cgcgagtcct atgcccatgc cccgatgccg cggatgacca
     2761 acaccttcat gcgcggcggc aacgacgatc cggccgaact gctgggccgg gtgaaaaacg
     2821 gcatctatgc caaggccttc ggcggcgggc aggtcgatat cgtctcgggc aagttcgtgt
     2881 tctcctgcac cgaggcctac agaatcgaga acggcaagct cggcgcaccg atcaagggcg
     2941 caaccctgat cggcgatggg ccttcggtcc tcacccgggt caccggcatc ggcaacgaca
     3001 tggcgcttga cgaaggggtc ggcgtatgcg gcaagggcgg gcagtcggtc ccggcgggcg
     3061 tcggccagcc gaccttgctg gtcgaaggcc tcactgtcgg cggcaccgcc tgaattcacg
     3121 accggaaagg accgccatga agcccgcatt cctcatcgcc gccgccgcag ccgtcctcgc
     3181 cggtcttgcc gcgcccgtcg ccctgagcgc cgccgaaccc cagccagcgg cggtggcggc
     3241 ggccccggcc ccccgcccct atccgcagct gactcacgtc gcctgggtcg tgcccaacct
     3301 tgacaaggcc ctcgccgacg gcgccgccca gctcggcacc aagccggcca acctggtatt
     3361 ccgccgggtg ctgacggtcg atcgtggcac ttaccagggc cggcccgccc acttctcggc
     3421 cgagttcgcg ctgctcgagc ttggcaatac ccagttcgaa ttcatccagc cgctgaccgg
     3481 gacttcgccc tattccgatg cgctcaaggg ccaccgcggc gccgtcctgc accacctggc
     3541 cttcaccgtt cccaatctcg atggccagat cgccgcggcg cggcgcgcca accctcaggc
     3601 cagggtggta ctggacgccc atctcaaggg gttcgacgcc cgctacgtct acatgagcgg
     3661 ggtcatgccc ggcactctgg tcgaattcat ccccgggggg taaatccggc ccggcgcaag
     3721 tcaggctccg ttcaactggc cgggtccaga ctgcgcggtc cgggcaatgt gcccggcaaa
     3781 aggaggacag catgaacaag gctatcgtgc ttctggcagc gggtgcttcg ctgctcgcgg
     3841 tgccggtcat ggccgccccg agactcacgg gcgaggccag gctggcagac atcctcaagg
     3901 ggcgggtccc gggcaagccg gtggactgca tcgatccccg gatcaacagc gatacccaga
     3961 tcgtcgacgg cacggccatc gtctatggct cgggccgcac ggtctatgtc cagcgccctg
     4021 ataatgcccg ttcgctgcgc agcgacgaca tgctggtgac cacgctgcgc ggctccagcc
     4081 agctctgctc gatcgacgtg gttcatctcc acgatcgctc cggcggttgg aaccggggct
     4141 tcgtcggcct caacaagttc gtgccctaca cccgcgaaca cgtcgcgcgt aactgaccgg
     4201 gcgcgcccgt ccttcggcga ccacttttga gtggcgccga aggtagccaa ccgtttgcaa
     4261 tttcactttc tatttatatc aatggcttag ccctgcgaat catgcaggga gaagccaagc
     4321 gcgatccacg gcgcgccaca ttccttaaag tccacgtgcg cagcgacaac ggctggtcgg
     4381 aggcggtgct ctgcaacgtt tcagcgcatg gcatgatgct gcgcggtgac gacctgcccc
     4441 gccgcggcaa cttcatcgag attgacagcg gcacggtcac ggtagtcggg caggtccgct
     4501 gggcgctcgc cggccgctgc ggcatccgca cccgcgaggc cgtcgacctc ggcgccctgc
     4561 tcggcgatca cccgcaaggc gaacggcatt ccgccccggc cggcccggtg gtccgctatg
     4621 ccgcgggccc ccgctcacgc ccggcgaccg atagcaaggc catggcccgg gcgctcgact
     4681 tcgggctgac cgtggccctg ctggccggcg gggcctggct gctgggcacg accgtgaccg
     4741 gcctgttcgg caccccgctc gagcaggtca aggccgagct cgcgccgcac cgcccggcag
     4801 gctgatcaca gccgcacctg gccttggacg cgccgcccac gcgcggcagc cgccttgccg
     4861 ctcacgcgcc cgagatcccg ccgttgcagc tgaccgcctg ccccgtgata tgcgccgcga
     4921 gcgggctggc gaggaacgtg atcagggcgg cgagatcgtc cggaccgggc aggcccagct
     4981 gggcacgctt ctcgatcttc tcgaacagct tcttcgaata gccgtcgtcc atcaccttgt
     5041 catagttgcg ggtgtccttg atcagcgagg gcgtcaccgc gttgagccgg atgccgtagc
     5101 gccccatctc gcgagcaaga ccgcgcagga acatgaccga tccggccagc gccccgccgt
     5161 gaaccgcttc gcccggcgtc ggcaccttgg ccgcgtccga agtcaccccg atcaccgccc
     5221 cgccgccgcg cgccttcagc atcggaatgg cgaaatggca gcagcgcacg aggctgagga
     5281 acacccccat gatcacctcg gccgattgcc ccggcgtcat gttctcgaac aggtcgggct
     5341 tgctgccgcc gccgccgtag ccggagtgga cgaagacatc gagcccgccg cacagccgcc
     5401 ccgtttcggc aaacagcgcc tcgatctcct ccaggctgtt catgtcggcg cgcacgaaat
     5461 gaaattgcgt ccccggcagt tccgccgcca gcgcatcgcg cacctgcgca ccttccgccg
     5521 ggttgcgact gttgatcacc acttcggcgc agccccattg cgccagcatc cgcgccgagg
     5581 cgcgccccac gcccgaggtt gatccggtga cgagcgccac cttgcccgcg aacagcttgt
     5641 cgaacatggc cgcgcccttc ctctccgcct gcgcccggcg cccggcgccc ggcggcagca
     5701 aggatgtcgc agaaccgccc gagggaaaag accccaccag aaccgtggcg ggtcatcgca
     5761 tccgtgcttg ccccccggcc ttgcccccgc caccatgtcg ctcaggggag acggcatggc
     5821 agaggccctg gacggggaat acgactacat cgtggtgggc gccggcaccg cc
//
LOCUS       DATNBV010000086         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_63530,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000086 DATNBV010000000
VERSION     DATNBV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63530"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VN627_02055"
     CDS             complement(<1..889)
                     /locus_tag="VN627_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HXD02069.1"
                     /translation="MNEVVLRPGDVGLSAWREVYRGAPVLLDPAAATAIAESAAAVAR
                     ILAKGEPVYGINTGFGKLASVRIEACDLATLQRNIVLSHAAGVGEPAPVPVIRLMLAL
                     KLASLAQGASGVAPETIALLEAMLAAGLTPIVPVKGSVGASGDLAPLAHMAATMIGVG
                     EIFVGEERRPAAKALAAAGLAPLTLGPKEGLALLNGTQFSTANGLAALFEAEVLFRSA
                     LVTGALSTEAAKGSDTPFDHRIHTLRRQPGQIEVADALRELMARSAIRASHLKDDERV
                     QDPYCLRCQPQVMGAALDVL"
     gene            complement(886..>2016)
                     /gene="hutI"
                     /locus_tag="VN627_02060"
     CDS             complement(886..>2016)
                     /gene="hutI"
                     /locus_tag="VN627_02060"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HXD02070.1"
                     /translation="DGIVAALDGRIVYAGPASEAPDLDAQIVEDCAGRWITPGLIDPH
                     THLVFAGDRAHEFELRLAGASYEEIARSGGGILSTVAATRAASEAQLVAAALPRLDAL
                     LAEGVTTIEVKSGYGLTLADELKCLRAARALAGARRVDIRTTFLGAHALPPEHAGNPD
                     GYIDLVCGQMIPAVAEAGLADAVDAFCEGIGFTPDQTRRVFEAARAHGLPVKLHAEQL
                     SNLGGAALAAGFGALSADHLEHLDEAGVAAMAKAGTVATLLPGAYYFVRETRPPPIAA
                     LRAAGVPMALATDCNPGTSPLTSPLLVMNMAATLFRMTVEECLAGVTREAARALGLAG
                     EVGTLEAGKRCDLAIWNIERPAELVYRMGFNPLRARVFGGQG"
BASE COUNT          304 a          696 c          738 g          278 t
ORIGIN      
        1 gcagcacgtc gagcgccgcg cccatcacct gcggctggca gcgcaggcaa taggggtcct
       61 gcacccgttc gtcgtccttc agatgcgagg cgcggatggc cgaccgcgcc atcagctcgc
      121 gcagggcgtc ggccacctcg atctggcccg gctgacgacg cagggtgtgg atgcgatggt
      181 cgaagggggt gtcggaaccc ttggccgcct ctgtcgagag cgcgcccgtc accagggccg
      241 aacggaacag cacttcggct tcgaacaggg cggccagccc attggccgtc gagaactggg
      301 tgccgttgag cagcgccagc ccctccttgg ggccgagggt caagggcgcg aggccggccg
      361 ccgccagcgc cttagcggcg ggccttcgct cctcgccgac gaagatctcg ccgacgccga
      421 tcatggtcgc ggccatgtga gctagcgggg ctaaatcgcc cgaggctccg accgagccct
      481 tgaccggcac gatcggggta aggcccgcgg ccagcatggc ttccagcagc gcgatggtct
      541 ctggcgctac gcccgaggcg ccttgggcca ggctcgcgag cttaagggcg agcatcaggc
      601 ggatgacggg aactggcgcg ggctcgccca ccccggcggc gtgcgacagg acgatgttcc
      661 gctgcaaagt cgccaggtcg cacgcctcga tccggacgct ggccagcttg ccgaagccgg
      721 tgttgatccc atagaccggc tcgcccttgg ccaggatgcg ggcgaccgcg gccgcgctct
      781 ccgcgatggc cgtggcggcg gcagggtcca gcaggaccgg cgcgccgcga tagacttcgc
      841 gccaggctga aaggccaacg tcccccggcc gcagcaccac ctcgttcatc cttggcctcc
      901 aaagacgcgc gcacggagcg ggttgaaccc catgcggtag accagctcgg ccggccgttc
      961 gatgttccag atggcgaggt cgcagcgctt gcccgcctcc agggtcccaa cctcgccggc
     1021 cagccccagg gcgcgggcgg cttcgcgggt caccccggcc aggcattcct cgaccgtcat
     1081 gcggaagagg gtggccgcca tgttcatcac cagcagtggc gaggtgagcg gcgaggtgcc
     1141 gggattgcag tcggtggcga gcgccatcgg cactccggcg gcgcgtaggg ccgcgatcgg
     1201 cggaggcctg gtttcgcgca cgaaatagta ggcgccgggc agcagggtcg cgaccgtgcc
     1261 ggccttagcc atggccgcga caccggcctc gtccagatgc tccagatggt cggccgacaa
     1321 tgcgccgaac ccggcggcca gcgccgcgcc ccccaggttc gagagctgct cggcgtggag
     1381 ttttaccggc agcccgtggg cgcgcgctgc ctcgaacacc cgtcgggtct gatcgggggt
     1441 gaacccgatg ccctcgcaaa acgcatccac cgcgtcggcc aggcccgcct ccgcgacggc
     1501 ggggatcatt tggccgcaga ccaggtcgat gtagccgtcg ggattgcccg catgttcggg
     1561 cggcagggcg tgcgcgccca ggaaggtggt gcggatatcg acgcggcgtg cgccggcgag
     1621 ggcgcgggcg gcgcgcaggc acttgagctc gtccgccagg gtcaggccgt agccggactt
     1681 cacctcgatt gtggtcacgc cctccgccag cagggcgtct aaacggggga gggcggcggc
     1741 gaccagctgg gcttccgagg ccgcgcgcgt ggccgcgacg gtggagagga tgccgccgcc
     1801 ggaacgagcg atctcctcat agctcgcgcc cgccaggcgc agctcgaatt cgtgggcccg
     1861 gtcgccggcg aagacgagat gggtatgagg gtcgatcagg cccggcgtga tccagcggcc
     1921 cgcgcaatcc tcaacaatct gagcgtcgag gtccggagcc tcggaggcgg gcccggcgta
     1981 gacgatccgg ccatccaggg cggcgacgat tccgtc
//
LOCUS       DATNBV010000087         5251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1323771,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000087 DATNBV010000000
VERSION     DATNBV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323771"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..180
                     /locus_tag="VN627_02065"
     CDS             1..180
                     /locus_tag="VN627_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXD02071.1"
                     /translation="MRHDPDFGAALAAHGRATIHARHTCAHRVDELLGIYAQLTSARP
                     VQTNSTPTPLRSCRV"
     gene            268..1371
                     /locus_tag="VN627_02070"
     CDS             268..1371
                     /locus_tag="VN627_02070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXD02072.1"
                     /translation="MKIAFYGSSLLSAWWNGAATYYRGVLKALAARGYQITFYEPDAF
                     DRQQHRDIDPPDWCRSVVWEATPEGLAKVVAEAARADVVIKASGVGVYDAQLLEGVMA
                     AARPDAIRLYWDVDAAASLDEMAESDLHPVRRALRSLDMVLTYGGGPPVIDRYKEFGA
                     IDCAPIYNALDPETHHPVPVLRTLTADLAFLANRLPDREARVEEFFLKAAALAQGKHF
                     LLGGNGWGDKPMPPNVRQLGHVGTNRHNAFNCSPLAVLNVARDSMARVGFSPATRVFE
                     AAGAGACLITDEWEGIELFLEPGEEVLVARDGQDVADLLHQLTPERARAIGKAALARV
                     LSQHTYDRRGAEVDGVLRMLESRARVGQREVAA"
     gene            1368..2417
                     /locus_tag="VN627_02075"
     CDS             1368..2417
                     /locus_tag="VN627_02075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXD02073.1"
                     /translation="MKLVVLGLTLSSSWGNGHATTFRALLSAFAARGHQVLFLERDRP
                     WYADHRDLPAPDFCHLAYYDSPAELEGWREPVAEADAVIVGSYVPDGVAVGEWAQSVA
                     PGRTCFYDIDTPVTLAKLARGDFEYLTPGLIPGYATYFSFTGGPVLTRLEREYGSPRA
                     RALYCSVDTARYRPQDVPKRWDLSYLGTYSDDRQPTLARLLIDVAEACPKRRFAVAGP
                     QYPAAIRWPANVERIEHLPPAAHPEFYSASRFTLNVTRADMIAAGWSPSVRLFEAGAC
                     ACPIISDQWAGIETVLPGIRLAETGADVLAILAGDAEGAGEAARAAGLARHSAAVRGA
                     ELEADLLALTEPATA"
     gene            complement(2420..2899)
                     /locus_tag="VN627_02080"
     CDS             complement(2420..2899)
                     /locus_tag="VN627_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HXD02074.1"
                     /translation="MSSLPPRSVACPAVPVSWGELLDKITILEIKRDRIAAPTARANI
                     LRELGVLARVAAAVEGVGGLAALMFRLKAVNRDLWEIEDAIREEEARGRFGRRFVGLA
                     RAVYRTNDRRAAIKREINGLLGSALVEEKSYAGRFETGPLPAASAPPQHERSSIRSI"
     gene            complement(2896..3771)
                     /locus_tag="VN627_02085"
     CDS             complement(2896..3771)
                     /locus_tag="VN627_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02075.1"
                     /translation="MHPIAPCASAPDWTAAMRAGDWERAWTLSESVLFTRDPATRDDP
                     RLPYHLRWVWDGTPPDGRHVLVRCYHGLGDVLQFARYLPELARRAASVTVEVCDRLIC
                     LLDDFPVRLVPFDQARPLPRSDCDIEIMELALALRIAPDAGESEWLRTDGFTFPAGTT
                     GLCLSSGEWDPARSVDPEWFSASPLQRPCIALDLGCSSLAVLNPGGCPADLAATASFI
                     AGCEAIVTVDTMVAHMAGSMGKPTWLMLRDEPDWRWVPGAETSAWYASLRLVHQREAG
                     DWAPVVAQVRTEVGL"
     gene            complement(3821..4264)
                     /gene="trxC"
                     /locus_tag="VN627_02090"
     CDS             complement(3821..4264)
                     /gene="trxC"
                     /locus_tag="VN627_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HXD02076.1"
                     /translation="MSEALIVACPACATRNRVPATRLGSDPTCGTCHKPLFNKYPVEL
                     NSSNFAAHADKSDVPLLIDFWAEWCGPCRQMAPAFAAAAARLEPRVRLGKLDTEAEQQ
                     LAARFGIRSIPTLVLVKRGREIARQSGAMPPDAIVRWAEQALASH"
     gene            complement(4309..4995)
                     /locus_tag="VN627_02095"
     CDS             complement(4309..4995)
                     /locus_tag="VN627_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXD02077.1"
                     /translation="MKLAILDKDRPTREAHRALAQSLGFHCAPFQTGRELMRSLQRDG
                     HDLFMVDWDLPDLNGPGLLEGIRNQVGILPPLLLLTAPLTSGDVARALAGGADCVLAK
                     PCAAEVLVAQLRAMARRSTNCGAVSTETYGGHTFDLGTRRLLVNGAALHLTPKEFTLA
                     LLLFRNLNRAVSRSYILETVWGLDPAVTTRTLDIHISKIRNKLGLRIEGGYRLSPVYG
                     FGYRLDEYAG"
BASE COUNT          876 a         1887 c         1622 g          866 t
ORIGIN      
        1 ctgcgccacg atcccgattt tggcgcggcc cttgcggccc acggtcgcgc taccatccac
       61 gcccgccaca cctgtgccca ccgcgtcgat gagctgctgg ggatctacgc gcagctaacg
      121 tcggcgcgcc cggtgcaaac caactccaca cccactcccc tccgttcgtg tcgagtctag
      181 tcgagacaca ccgcacccgt ctttcgactt tgcccgaggc gaacgggcga ggggcctcgt
      241 agaccgcaag cccaaggagc aatccgcatg aaaatcgcct tctacggctc cagcctgctc
      301 tccgcctggt ggaacggcgc ggcgacctat tatcgaggcg tgctgaaggc cctggccgcc
      361 cgcggctacc agatcacctt ctacgaacca gacgccttcg accgccagca gcaccgcgac
      421 atcgatccgc ccgactggtg ccgctcggtc gtctgggaag ccacacccga aggcctcgcc
      481 aaggtcgtcg ccgaagctgc tcgcgccgat gtggtgatca aggccagcgg cgtcggcgtt
      541 tacgatgccc agctgctcga aggcgtcatg gccgccgccc gccccgatgc gatccggctc
      601 tactgggatg tcgacgccgc cgcctctctc gacgagatgg ccgaaagcga tctccatccc
      661 gtccgccgcg ccctgcgcag cctcgacatg gtcctcacct atggcggcgg ccctccggtg
      721 atcgaccgct acaaggaatt cggcgccatc gattgcgcgc cgatctacaa cgcgctcgat
      781 cccgaaaccc accaccccgt tccggtcctg cgcacgctga ccgccgatct cgccttcctc
      841 gccaatcgcc tgcccgaccg agaagcgcgg gtggaggaat tcttcctcaa ggccgccgcc
      901 cttgcgcagg gcaagcactt cctcctgggc ggcaacggct ggggtgacaa gccgatgccg
      961 cccaatgtcc gccagctcgg ccatgtcggc accaaccgcc acaatgcctt caactgctcc
     1021 ccgctcgccg tgctcaacgt cgcccgcgat tccatggcgc gggtcggctt ctcccccgca
     1081 acccgggtgt tcgaggccgc gggggccggc gcctgcctga tcaccgatga atgggaaggg
     1141 atcgaactgt ttctggagcc cggcgaagaa gtcctcgtcg cccgcgacgg gcaggatgtc
     1201 gccgacctgc tccaccagct gacccctgaa cgcgcccgcg ccatcggcaa ggctgctctc
     1261 gcccgcgtcc tgtcgcagca cacctacgac aggcgcggag ccgaggttga tggcgtcctg
     1321 cggatgctcg agtcccgggc gcgtgttgga cagcgtgagg tggcggcatg aagctggtcg
     1381 tcctgggcct caccttgtcc tcgtcgtggg gcaacggtca tgccacgacc ttccgtgccc
     1441 tgctctcggc cttcgcggcg cgcggccacc aggtcctgtt cctcgaacgc gacaggccgt
     1501 ggtacgctga ccaccgcgat cttcccgcgc ccgatttctg ccacctggcc tattatgaca
     1561 gccccgccga gctggagggc tggcgcgaac cggtggccga ggccgatgcc gtgatcgtcg
     1621 gctcctatgt ccccgatggc gtcgccgtcg gcgaatgggc ccagtcggtc gccccgggcc
     1681 gcacctgttt ctacgatatc gataccccgg tcaccctggc caagctcgcc cggggcgatt
     1741 tcgaatacct gaccccgggg ctgatccccg gctatgccac ctatttctcc ttcactggcg
     1801 gcccggtgct cacccggctc gaacgcgaat acggcagccc gcgcgcccgc gcgctctact
     1861 gctcggtgga taccgctcgc tatcgcccgc aggacgtgcc caagcgctgg gacctaagct
     1921 acctcggcac ctattccgac gatcgccagc ccaccctcgc gcgcctgctg atcgacgtgg
     1981 ccgaggcctg tcccaagcgc cgcttcgccg tcgctggccc acaatatcca gccgcgatcc
     2041 gctggcccgc caacgtcgaa cggatcgaac atctgccgcc ggccgcccac ccggaattct
     2101 attccgcctc gcgtttcacc ctcaacgtca cccgcgccga catgatcgcg gcgggatggt
     2161 ccccctcggt gcgcctgttc gaagccggcg cctgcgcctg cccgatcatt tccgaccagt
     2221 gggctggcat cgaaaccgtc ctccccggca tccgcctcgc ggaaaccggg gctgacgtct
     2281 tggcaatcct tgccggcgat gccgaagggg caggggaggc cgcaagagcc gcaggtctgg
     2341 cccgccacag cgcagccgtg cgcggcgccg agctggaagc cgatctgctg gcgctcactg
     2401 aaccggcgac tgcctgagct tagatcgacc tgatgctact ccgttcgtgc tgaggagggg
     2461 ccgaggccgc aggcagcggc cccgtctcga agcgccccgc ataactcttc tcctccacca
     2521 gcgccgatcc cagcaacccg ttgatctccc gcttgatcgc cgcccgccgg tcgttggtcc
     2581 ggtaaaccgc ccgggccagc cccacgaacc gcctgccaaa ccgcccccgc gcctcttcct
     2641 cgcggatggc gtcctcgatc tcccacaggt cgcggttcac cgccttcagc cggaacatca
     2701 gcgcggcgag cccgcccacg ccctccaccg ccgccgccac ccgcgccagc acgcccagct
     2761 cgcgcaggat attggcccgg gccgtcggcg cggcgatccg gtcgcgcttg atctcgagga
     2821 tggtgatctt gtcgagcagc tcgccccacg aaacgggcac ggccggacaa gcgacggatc
     2881 ggggcggcag gctgctcaca gtcccacctc cgtgcgcacc tgcgccacca ccggcgccca
     2941 gtcccccgct tcgcgctggt gcaccagccg cagcgaggca taccacgcgc tggtctccgc
     3001 cccgggcacc cagcgccagt ccggctcgtc gcgcagcatc agccaggtcg gcttgcccat
     3061 cgacccggcc atgtgggcaa ccatcgtatc gaccgtcacg attgcctcgc acccggcgat
     3121 gaaactggcc gtcgcggcca gatccgccgg gcatccgccg gggttcaaga ccgccaggct
     3181 agagcaaccg aggtccagcg cgatgcaggg gcgttgcagt ggacttgcac tgaaccattc
     3241 cggatcgaca cttcgcgcag gatcccattc gcccgaactc aggcacagcc ccgtcgtgcc
     3301 cgccggaaag gtgaaaccat ccgtccgcag ccattcagat tcgcccgcat caggcgcgat
     3361 gcgcagggca agggccagtt ccatgatctc gatgtcgcag tccgaccggg gcagggggcg
     3421 agcttgatcg aatggcacca gcctgactgg aaaatcgtca agtaaacaga ttagtcgatc
     3481 acatacttca accgtcaccg aagctgcgcg gcgggccagt tcaggcaggt aacgggcaaa
     3541 ctgcagcacg tcgccgagcc cgtgatagca ccgtaccaat acgtggcgcc cgtcgggggg
     3601 cgtgccgtcc cacacccagc gcaggtgata gggcagccgc ggatcgtcgc gcgtcgcggg
     3661 gtcgcgggtg aaaagcacgg attcactgag ggtccacgcc cgctcccagt ccccggcccg
     3721 catggcggcg gtccagtccg gggcggaggc gcatggggca atcgggtgca tggcaaagcg
     3781 acgcgcgccg cccggccatg ttcccatcaa aggagtaaaa tcagtgggat gccaatgctt
     3841 gttcagccca tctcacgatt gcgtcgggcg gcatggcccc ggattgccgg gcaatctcgc
     3901 ggccccgctt caccagcacc agggtgggga tcgaacggat cccgaaccgg gccgcaagct
     3961 gttgttccgc ctcggtatcg agcttgccca gccggacccg tggctccagc cgcgcagcgg
     4021 cggcggcaaa ggccggggcc atctgccggc aaggcccgca ccattccgcc cagaaatcaa
     4081 tcagcaaggg cacgtcgctc ttgtcggcat gggccgcgaa gttgctgctg ttcagctcga
     4141 ctggatactt gttgaacaat ggcttgtggc atgtcccgca agtagggtct gaaccaagcc
     4201 gcgtggcggg cacgcggttg cgggtggcgc aggcggggca ggcgacgatc agggcttcgc
     4261 tcatgcccgc aatatgggaa cgccgctccg gcttgcaaac ccgccggttc agccggcgta
     4321 ttcgtccagc cggtagccga agccgtagac cgggctcagg cgatagccgc cctcgatccg
     4381 caagccgagt ttgttacgga tcttcgaaat gtggatgtcg agcgtccggg tggtcacggc
     4441 cgggtcaagt ccccacacgg tttcaaggat atagctgcgc gaaacggccc ggttgaggtt
     4501 gcggaacagc agcagggcca gggtgaattc cttgggggta aggtgcagcg ccgcaccatt
     4561 caccagcagc ctgcgggtgc ccaggtcgaa cgtgtggcca ccataggttt cggtgcttac
     4621 cgcgccgcag ttggtcgagc ggcgcgccat cgcccgcaac tgcgcgacca gcacttccgc
     4681 ggcgcagggc ttggccagca cgcaatcggc cccgcctgcg agcgcgcgcg ccacgtcgcc
     4741 gctggtcagc ggcgcggtca gcagcagcag gggcggcaag atcccgacct ggttgcgaat
     4801 accctccagc aagcccgggc cgttcaggtc gggcaggtcc cagtcgacca tgaacaggtc
     4861 gtgcccgtct cgctgaagcg agcgcatcag ttctcgcccg gtctggaacg gggcgcaatg
     4921 gaagcctaag ctctgcgcca gcgcccgatg ggcttcgcga gtgggcctgt ctttgtcgag
     4981 gatggcgagc ttcatgggca aacggccgtt gagcggcctt gatcctcagt tctgttatgc
     5041 gctcccatca ttgtcttagc agataaattc cggggcgcag gagtgacgtg agtaagttta
     5101 caatttttca ccatgcaagg ttttgatgga aaaccttgcc cgcgaaatgc gcaaaaactc
     5161 aggtactcta cttgaactgc gcatgaatac tgagggtgtg ctctcggtac cgtcgccagg
     5221 tggtgataga ctggctcaca tgctctcgat c
//
LOCUS       DATNBV010000088         3270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1015864,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000088 DATNBV010000000
VERSION     DATNBV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015864"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VN627_02100"
     CDS             <1..556
                     /locus_tag="VN627_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02078.1"
                     /translation="GWRLDAIHLFDAAGVEPWDCGGFAASLAHGLGALARQASLILVG
                     REYADYDDGSVPPALARALGLEHVNLALAVHSDRGSTWVSRQRGATIERLRLAGPAVL
                     SVTNDSHNRLRHPLLKNVMAARTVQFTTLAGTAASPAAQVSLVSAEPVVPLPRAIACR
                     LLHGTPQQQAEALALVLADARRAA"
     gene            553..1557
                     /locus_tag="VN627_02105"
     CDS             553..1557
                     /locus_tag="VN627_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXD02079.1"
                     /translation="MSTAAIRLTVVLPFAPDERQAQDSLIAAAGALLAGGPFSSLDIV
                     QLSPAAGAVQPRATAGAVTWWECICACATEQAPAETLAALVRAALAADGLRDMRQRLV
                     LLPAGPGAEELAALLAADFKGCSLGRCAHLQADGSTVTGRRPAFGARVWIELRTAAQF
                     CCATWRPETPGLASVQPLPNSAIRSLALSCDAPRREVELVQPADAQPRLEGAAAVVSG
                     GRGMDGPEGFALLARIASSLGAAVGGSLPAVDAGWVPVARQIGQSGKFVAPRLYFAVG
                     ISGTPQHLAGVSHETRIVALNKDPDAAIFSSAEVGAVGDWRDILPLLAQQLEARQPAG
                     "
     gene            1653..2573
                     /locus_tag="VN627_02110"
     CDS             1653..2573
                     /locus_tag="VN627_02110"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXD02080.1"
                     /translation="MEVFVRVAECGSFSRAAESLDLANATVTTCVRNLERHLDVTLIN
                     RDTRRLRLTEEGQMYLPRARELLQAVSQTEDEVRTRVGELRGWLHIETPISLGHALLC
                     PALPTFAQRYPEISAAITLTNQPHHMIERAIDVAIRMDHVEDADLVARPIYESPYVIC
                     CTPEVARTLPAHPGELDPRRCIGILPEERQHANMWSLQKGEERVELRPLGPLHFNSSD
                     ALLMAAESGVGVACVLDIFANRRLDGGSLVQVYPDWTTPVKTFYLVTAKARAGSAKVR
                     AFTDFLFEVLDSERRPSSRRAVTVKALGKR"
BASE COUNT          489 a         1113 c         1089 g          579 t
ORIGIN      
        1 cggctggcgg ctggacgcga tccacctttt cgatgcggcc ggtgtggaac cctgggactg
       61 cgggggtttc gcagcgtcgc tggcccatgg cttgggcgcg ttggcccgac aggcgagcct
      121 catcctggtc ggtcgcgaat acgccgacta tgacgatggc tccgtgccgc ctgcgctggc
      181 gcgcgcgctg ggcctcgagc atgtgaacct ggccctggcg gttcactcgg accgcggatc
      241 gacgtgggtg tcgcgacagc gcggcgccac gatcgagcgg ttgcggctcg ccggacccgc
      301 cgtgctgtcg gtcaccaacg attcgcataa ccgcctgcgc catcctttgc tgaagaacgt
      361 catggccgcc cgcacagtgc agttcaccac gctggccggc acagccgcca gtcccgctgc
      421 ccaagtcagc ctggtatctg ctgaaccggt tgtccccctg ccgcgggcaa tcgcctgccg
      481 cctgctgcac ggcacgccac agcagcaggc cgaggcgctg gcactggtgc tggccgacgc
      541 caggagagcg gcatgagcac cgccgccatt cgtctcacgg tggtcctgcc cttcgcgccc
      601 gacgagaggc aggcgcaaga tagcctgatc gctgcagccg gcgcgctgct cgccggcggc
      661 cctttttcgt cactcgacat cgtgcagctc agcccagcag caggcgcggt ccagccccgg
      721 gcgaccgccg gcgcagtgac ctggtgggag tgcatctgcg cttgcgccac cgaacaagcg
      781 cctgctgaga cgctcgccgc cctggtccgc gcagcgctcg cagccgatgg cttgcgcgat
      841 atgcggcagc gcctggtact gctgcccgcc ggaccgggcg cggaagaact cgcggcgctg
      901 ctcgcagccg acttcaaagg ttgttcgctg ggccgctgcg ctcacctcca ggcggacggc
      961 tccaccgtga ccggccggcg gcccgcgttt ggcgctcggg tgtggatcga gctgagaaca
     1021 gctgctcaat tctgctgcgc aacctggcgc ccggagacgc ctgggctggc ctccgtccag
     1081 ccgctgccga actcggccat ccgaagcctt gccttgagct gcgacgcgcc gcgccgtgag
     1141 gtggaactcg tccagcccgc cgacgcgcag ccgcgcctgg aaggcgccgc tgccgtggtg
     1201 agcggcggcc gcggtatgga tggcccggaa ggcttcgcat tgctcgcccg catcgccagc
     1261 agcctcggcg cggcggtggg gggcagcttg cccgcggtcg acgcgggctg ggtgccggtg
     1321 gcacggcaaa tcgggcaatc gggcaagttc gtggcgccgc ggctttattt cgccgtgggc
     1381 atttcaggca cgccccaaca cctggccggg gtgtcgcacg aaacgcggat cgtggcgctg
     1441 aacaaggatc cggacgccgc cattttcagc tcggccgaag tcggcgcggt gggcgactgg
     1501 cgcgacatcc ttccattgct ggcgcagcag ctcgaggcgc ggcagcccgc gggctgatgg
     1561 cggcggcggg cgccggggcg caggccggtg actacaattc ccggcaggca tccgtccgat
     1621 caaagaggtt cgatatcgac aggctctggg ccatggaagt gtttgtccgc gtggcggagt
     1681 gcggcagctt ttccagggcg gccgagtcgc tggacctcgc gaacgccacg gtgacgactt
     1741 gcgtgcgcaa cctcgagcgg cacctggacg tcacgctcat caatcgcgat acccgccggc
     1801 tgcgactgac ggaggaaggg cagatgtacc tgccccgggc gcgcgaactg ctgcaggcgg
     1861 tttcgcagac ggaagacgag gtgcgcaccc gtgtcggcga gttgcgcggc tggctgcaca
     1921 tcgagacccc gatctcgctg ggccacgcgc tgctttgtcc ggcgctgccg accttcgccc
     1981 agcgctatcc ggagatctcc gcggcgatca cgctgaccaa ccagccgcat cacatgatcg
     2041 aacgcgcgat cgacgtggcg atccgcatgg accatgtcga ggacgccgac ctggtggcac
     2101 ggccgatcta cgagagccct tacgtcatct gctgcacgcc ggaagtggcc aggacgttgc
     2161 cggcgcatcc gggcgaactc gatccccgtc gctgcatcgg catcctgccc gaggagcggc
     2221 agcacgcgaa catgtggtcg ttgcaaaaag gcgaggaacg cgtcgaattg cggccgctcg
     2281 gcccgttgca cttcaatagc agcgacgcgc tgctgatggc cgccgagagt ggcgtgggcg
     2341 tggcctgcgt gctcgatatc ttcgcgaatc gccggctgga cggcgggtcg ctggtgcagg
     2401 tctacccgga ctggaccacg ccggtcaaga ctttctatct cgtgacggcc aaggctcgcg
     2461 ccggctcggc caaggtgcgc gccttcaccg atttcctgtt cgaggtgctg gattccgagc
     2521 gccgccccag ctcgcgccgc gcggtcactg tgaaggcgct gggcaagcgc tgacgccaag
     2581 agttgtcatg ccggacttga gaagcctgcc ccggactccg atccgggggc atccatgctt
     2641 cctggattgc gggtcaggcc cgcaatgaca gcccttcttc ttgtcatgcc ggacttgaga
     2701 agcctgcccc ggactccgat ccgggggcat ccatgacttc ctggattgcg ggtcaggccc
     2761 gcaatgacat ctctcctctt gtcatcccgg gcttgacccg ggatcccttc ttcttgtcat
     2821 gccggacttg atccggcatc catgacttcc tggattgcgg gtcaggcccg caatgacatc
     2881 tcttctcttg tcatcccggg cttgacccgg gatccacggc ggacagcatg gagctcatca
     2941 ggcgaggcat ggattgcggg tcaagcccgc aatgacagcc cttcttcttg tcatgtcgga
     3001 tttgagaagc ctgccccgga ctccgatccg ggggcatcca tgacttcctg gattgcgggt
     3061 caagcccgca atgacagccc ccctcttgtc atcccgggct tgacccggga tcccttcttc
     3121 ttgtcatgcc ggacttgatc cggcatccat gacttcctgg attgcgggtc aggcccgcaa
     3181 tgacagccct tcttcttgtc atgccggact tgagaagcct gccccggact ccgatccggg
     3241 ggcatccatg acttcctgga ttgcgggtca
//
LOCUS       DATNBV010000089         3052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1016323,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000089 DATNBV010000000
VERSION     DATNBV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016323"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VN627_02115"
     CDS             complement(<1..842)
                     /locus_tag="VN627_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXD02081.1"
                     /translation="MTVPFTNLDKLYINNEWVKPESGDREAVLNPATEVTIGFAPLAG
                     LAETEAAIVAAREAFDNGPWPRMSFRERAAVMRRMHAVLTRRLPEIQALTIAEVGTTI
                     GLAHSIQTVLPLQHMMYGIEYAETIKPESGELIISPPWVPGMRDSLGLVTVVKEPWGV
                     VAGITGYNFPFLLNMAKVVPALLAGCTMVLKPSPFTPFSALLFGQIADEVGLPKGVLN
                     VVNGGLDCAKLLTEHKSIDMVTFTGSDKVGALIAAQAAPTLKKVHTELGGKSAMIVRA
                     DADLV"
     gene            complement(918..1145)
                     /locus_tag="VN627_02120"
     CDS             complement(918..1145)
                     /locus_tag="VN627_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HXD02082.1"
                     /translation="MKVVIDRAKCVGHARCQAVAPELYPLDDLGYIDSDGFDVPPGME
                     AAARNGAKACPERIIQTIGDPSGKDWPPAKA"
     gene            complement(1406..>3052)
                     /locus_tag="VN627_02125"
     CDS             complement(1406..>3052)
                     /locus_tag="VN627_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HXD02083.1"
                     /translation="LGYQAFRYHSREIRLPVLLAHAAGFLSIMTAAILVSFGHFHHAA
                     MNLWQVLGPWLVMPGLFLLVVVRRDWQAWRVLSAGLLPKLGESSYSLYLLHPLAIALA
                     YAWLPGAAAAPAAVIGAVVAARAGFRWIEQPGIAFGRWLMQPPPAGADERPARIGWLD
                     AARGCGIVLVVFGHALGGLIDSRLGAGLTAFRWLFFAIYTFHMPLFFLLAGLMVPHRL
                     ERDAAGFVRGLGTGIVAPYFLWSALQFTVIAALGTAVNNPVSGYWPTILALPWKTVSQ
                     FWFLYVLFWLHLIAAAVVPRWGAAVLLVLAVAMKLVAPMLGAPVVVRLVCAHALFYAI
                     GVVLRPDGVERLVIGQSRAHRVVVLVALALSGLVATFVMLPWFGANLPFATASSPQLA
                     NLAWRLPVLPVAGIAVVACLGLAGLPQVAGNRALAALGRLTMPVFVMHVLFVAGSRRC
                     TMPDLALCGHESVRSVQPRPGNRVRSRRGTGPASGAVGRSWRGQWRHRRGRSRGCGDW
                     KRCARTVRQARHDHGRPPDRRRAAGTWHRRGRSRRTCRLP"
BASE COUNT          625 a         1055 c          924 g          448 t
ORIGIN      
        1 accaggtcgg catcggcgcg aacgatcatg gccgacttgc cgccaagctc ggtatgcacc
       61 ttcttcaacg tgggcgcggc ctgcgccgcg atcagcgcgc caaccttgtc cgagccggtg
      121 aaggttacca tgtcgatcga cttgtgctcg gtcagcagct tggcgcaatc gagcccgcca
      181 ttgactacgt tgagcacgcc cttgggcagg cccacctcgt cggcgatctg gccgaacagc
      241 agagccgaga acggtgtgaa cggcgaaggc ttgagcacca tggtgcagcc ggccagcagc
      301 gcggggacaa ccttggccat gttgagcagg aacgggaagt tgtagccggt aatgcccgcc
      361 acgacgcccc acggctcctt taccacggtc accagtccga gcgaatcgcg catccctggc
      421 acccacggcg ggctgatgat cagttcgcca ctttcgggct tgatcgtctc ggcatattcg
      481 atgccataca tcatgtgctg aagcggaagg accgtctgga tcgagtgcgc cagaccgatg
      541 gtcgtgccca cttccgcgat cgtcagcgcc tggatctcgg gcaggcggcg ggtcagcacg
      601 gcatgcatcc tgcgcatcac tgccgcacgc tcgcggaacg acatccgcgg ccacgggcca
      661 ttgtcgaagg cctcgcgcgc cgcaacaatc gccgcttcgg tttccgccag gcccgccagc
      721 ggggcgaaac cgatggtaac ctcggtagcc gggttgagca cggcttcccg gtcacccgat
      781 tcgggcttca cccattcatt gttgatgtag agtttgtcga ggttcgtgaa cggaacggtc
      841 atgcgggatc actcgcaaaa agcaggtgcg aagctggtgc atcgcgaccg caggtaattt
      901 cgatagggaa ttgacgctca ggccttcgcc ggaggccagt ccttgccgga aggatcaccg
      961 atggtctgga taatccgctc ggggcaggcc ttggcgccgt tgcgggctgc tgcttccatg
     1021 ccgggcggta cgtcgaaccc atcgctgtcg atgtagccca gatcatcgag cggatagagc
     1081 tcgggcgcaa cggcctggca tcgggcatgg ccaacgcact tggcgcggtc gatcacgact
     1141 ttcatggtct ctcctccatc tcttccagac gcgcttgaat tctcgattct gcgcgactga
     1201 actatccagt tcagtatgtg gtcgcacgcg ccgcgtcaat cgccgcactg cacgcccgcg
     1261 aaccaacgcc cctccaggtt aacgcattcc tgccgcgctt ccccgggaat gcacggcccg
     1321 gccgctaaag gtggatttca gccatccatt ctagacccgc agatcacagt tcattctggc
     1381 ggtgacccca tggcagcaga cgacattacg gcaatcggca ggttcttcga gaccgaccgc
     1441 gacgatgcca agtcccggca gcacgtcgcc gatctggtgg ccgcccgtgg tcgtgccgcg
     1501 cttgacgcac tgtacgagcg catcgcttcc aatccccaca cccgcggctt cttccccgac
     1561 gatgccgcca ctgaccgcgc caagaacgcc caactgcgcc actggctggc cctgttccgc
     1621 gacgggatct cacccgatta ccaggcctcg gctgaacgga tcggacagac tcatgcccgc
     1681 atagggctag atccggcatg gtacatcggc ggctacccgc gacgaacagc acgtgcatca
     1741 cgaacaccgg catggtgagc cgccccagtg ccgccagcgc tcggtttccc gccacctgcg
     1801 ggagaccggc caggcccagg cacgccacaa cagcgatccc ggcaaccggc aggaccggca
     1861 gccgccacgc caggtttgcc aactgcggcg aacttgccgt tgcaaagggc aggttggcgc
     1921 cgaaccacgg cagcatcacg aacgtcgcga caagccccga aagggccagc gccacgagga
     1981 ccaccacccg atgcgcccgc gactgcccga tgaccagccg ctctacgcca tccggccgca
     2041 acaccacgcc gatggcgtaa aacagcgcgt gggcacaaac gaggcgtacg acaaccggcg
     2101 cgccaagcat tggtgccacc agcttcatcg ccaccgccag caccagcagc accgccgccc
     2161 cccaacgtgg aacgacggcg gcggcaatca ggtgcagcca gaacagcacg tagagaaacc
     2221 agaactgcga aacggttttc cacgggaggg ccaggattgt cggccaatag ccggacaccg
     2281 gattgttcac cgcggtgcca agcgcagcga tgaccgtgaa ctggagggcc gaccacagaa
     2341 agtagggcgc cacgatcccc gtcccaaggc cccggacgaa gccggcggca tcccgctcca
     2401 atctatgcgg caccatcaga cccgccagca ggaagaacag cggcatatgg aaggtgtaga
     2461 tggcaaagaa cagccaacgg aaagcagtca ggcccgcccc gagccgggaa tcgatcaacc
     2521 cgcccagggc atggccaaaa accaccagga caatgccgca gccccgggca gcgtccagcc
     2581 agccaatccg cgccgggcgc tcatcagcgc cggctggcgg gggctgcatc aaccagcgcc
     2641 cgaaggcgat gcccggctgc tcgatccatc ggaacccggc ccgggccgcc acgaccgcgc
     2701 cgatcactgc cgccggcgca gctgctgctc ccggcagcca ggcataggcc aacgcgatcg
     2761 ccagcggatg cagcagatag agggagtagc tcgattcccc caacttcggc aggagcccgg
     2821 cggagaggac ccgccaagcc tgccagtcac gccgcaccac caccagcaga aacaggcccg
     2881 gcatcacgag ccacgggccg agaacctgcc acaggttcat tgccgcatgg tggaaatgtc
     2941 cgaacgagac caggatcgca gccgtcatta tcgacaggaa gcctgccgcg tgggccagca
     3001 agaccggcag ccggatctcc cgggaatgat agcggaatgc ctggtagccg ag
//
LOCUS       DATNBV010000090         2000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1967410,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000090 DATNBV010000000
VERSION     DATNBV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967410"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VN627_02130"
     CDS             <1..855
                     /locus_tag="VN627_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02084.1"
                     /translation="HHLKCMDQRLPEPLDHYRTLIATLAHLSGTHKAGRLGAAAERDF
                     PFDREAAIVDRRNPFDTEQLTMRIERLVNFIRQYPHLFPANIAEPGFLATFTADAPRV
                     VAQQDAISRHQLANSDMIALCHWNGNIDNAWFWREQDGELRCGLIDWGSVGQMHVAQT
                     IWGCLSACEPDFIDANLPALIDLFTTEYARSGGPALDPRLLEQELELHIMTNSLRSLM
                     IAPRAILSEVPDPSQVADRYDPIFTVNETARVMVKVTTNFLNFWQRRDLGRWLRSGQF
                     ETEPEPAT"
     gene            complement(862..>2000)
                     /locus_tag="VN627_02135"
     CDS             complement(862..>2000)
                     /locus_tag="VN627_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HXD02085.1"
                     /translation="RDCGTLAIVLDHSKLDQPLTDWISLMPGCDRLGAGPFREVEGEP
                     GLEILSSGTTGKPKRVIFPTRMLVRMVESVLAGREGPMQPDILSWPYGGIGGMCNIVA
                     SAMLDRYTCVLEKFNVAEWVEGVERLKPTSVSGVPAMARMILDAKVPKERIASIKVFS
                     GGSAPMTPELQAAFEETYGIDVIWGYGATEFCGTVISWTPQLYREYRKAKLGAMGKAL
                     PGISLRVTDVDTGEELPVGAEGYLEVNVPVIGDHWIKTTDIVMIDDDGFVFHKGRGDG
                     AILRGGHKVLPEKVVDALRSHPAVLDAAVVGLPDERLGALPVSAVELREGASATPEEL
                     REYVRDMLTSPQVPARILIVPALPRTTSLKVDLKAVTAMFAADA"
BASE COUNT          356 a          695 c          606 g          343 t
ORIGIN      
        1 caccacctca agtgcatgga ccagcgcctg cccgagccgc tggaccatta ccgcacgctg
       61 atcgccacgc tcgcgcacct ttccgggacc cataaggcgg ggcggctggg cgcggcggcc
      121 gagcgggact ttcccttcga ccgtgaggcg gcgatcgtcg accggcgaaa tccgttcgac
      181 accgagcagc tgaccatgcg gatcgagcgc ctggtcaatt tcatccggca atatccacac
      241 ctgttcccgg cgaacattgc cgagcccggg ttccttgcca ccttcaccgc cgacgctccg
      301 cgggtcgtcg cccagcagga tgcgatcagc cggcaccagc tggccaattc cgacatgatc
      361 gccctgtgcc actggaacgg caacatcgac aacgcctggt tctggcgcga gcaggatggc
      421 gagctgcgct gcggcctgat cgattggggc agcgttggcc agatgcacgt agcccagacc
      481 atctggggct gtctctcagc ctgcgagccc gacttcatcg acgcgaacct gccggccctg
      541 attgacctgt tcacgaccga atatgcccgg tccggaggcc ctgcacttga cccgcgcctc
      601 ctggaacagg agcttgaact tcacatcatg accaacagcc tcaggtcgct gatgatcgcg
      661 ccgcgggcaa tcctgagcga ggtgcccgat ccctcgcagg tggcagaccg ctacgacccg
      721 atcttcacgg tgaacgagac cgcgcgggtg atggtcaagg tgacgaccaa ctttctaaat
      781 ttctggcagc gacgcgactt ggggcggtgg ctgcggtccg gccagttcga aaccgagccg
      841 gaaccagcca cctgaagccg atcaggcgtc agccgcgaac atcgcggtca cggccttgag
      901 gtccaccttg agcgaggtgg tgcgcggcag cgcaggaaca atcaggatcc gggcgggcac
      961 ctgcggcgaa gtcagcatgt cgcggacata ctcgcgcagt tcctccggtg ttgccgaggc
     1021 gccctcgcgc aattcgaccg cgctgaccgg aagcgctccc agccgttcat ccggcagccc
     1081 gacaacagcc gcatcgagca cggccgggtg gctgcgcagc gcatcgacca ccttttcagg
     1141 caggaccttg tggccaccgc gcaggatcgc gccgtcgccc cggcccttgt gaaaaacgaa
     1201 gccgtcatcg tcgatcatca cgatgtcggt cgtcttgatc cagtgatcgc cgatcaccgg
     1261 cacattcact tcgagatagc cttcggcgcc caccggcagt tcctcgcccg tgtcgacatc
     1321 ggtgacgcgc aggctgatcc ctggcaaggc cttgcccatc gcgcccagct tggccttgcg
     1381 gtattcccgg tagagctgcg gcgtccagct gatgaccgtg ccgcagaact cggtcgcgcc
     1441 gtagccccag atcacgtcga tcccataggt ttcctcgaac gcggcctgca gttccggcgt
     1501 cattggcgcg gagccgccgc tgaacacctt gatcgaggcg atccgctcct tcggcacctt
     1561 ggcatcgagg atcatgcgcg ccatggccgg cacgccgctg acggaggttg gcttcagccg
     1621 ctcgacgcct tcgacccatt cggcaacgtt gaatttctcg agcacgcagg tgtagcgatc
     1681 gagcatggcg ctggcgacaa tgttgcacat gccgccgatg ccgccatacg gccatgacag
     1741 gatatccggc tgcatcggcc cctcgcgacc ggccaacacg ctttcgacca tgcgcaccag
     1801 catgcgggtg gggaagatga cccgcttggg cttgcccgtg gtcccgcttg acaggatttc
     1861 gagaccgggc tccccctcta cctcccggaa cggccctgct ccaagccgat cgcacccggg
     1921 catcagggaa atccagtcgg tgagcggctg atccagcttg gagtgatcca gaacgatcgc
     1981 cagcgtcccg caatcccgcg
//
LOCUS       DATNBV010000091         3237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3556944,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000091 DATNBV010000000
VERSION     DATNBV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3556944"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="VN627_02140"
     CDS             complement(<1..150)
                     /locus_tag="VN627_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD02086.1"
                     /translation="MVTWPKEYGGRGLDLIQWLIFEEEYYRAGAPGRVNQNGIFLLGP
                     TLMEFG"
     gene            complement(313..1062)
                     /locus_tag="VN627_02145"
     CDS             complement(313..1062)
                     /locus_tag="VN627_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HXD02087.1"
                     /translation="MPITLTVRDRIAEILFDHPPVNAFDTKGWESIPAMIEQAGRDED
                     VRVLLIRANGRGFCGGVDIKEMQAHPERIAKLNRDNYLTFKAIRDCPIPVVGALHGFI
                     IGGGVNIAGACDSLIAAEGAFFSLPEIDRGAMGGAAFMSRMLPVQKVRTNFFTGGRVP
                     VEDLYRHGSIEKIVPLDQLEAEARALCEIIAGKSRTALVIAKQALNLIEPWNPSDMYR
                     IEQGFTLEMYAHEDSQKARDAFVEGKGAAKF"
     gene            complement(1077..2183)
                     /locus_tag="VN627_02150"
     CDS             complement(1077..2183)
                     /locus_tag="VN627_02150"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXD02088.1"
                     /translation="MTSALQTVLTERLGCRAPIIQTAMGWVAEPSLVIGSSNAGAFGF
                     LGAAVMTPDECREKILAVRKGTDKPFGVNFHSFQPGAEQIVDLILANAEQVRAVSFGR
                     GPNAKMIGRFKDAGILCIPTVGALKHARKMEELGVDMVNVQGGEGGGHTGSVPTTILL
                     PQVLDAVKVPVIASGGFADGRGLVAALAYGAVGIAMGTRFLLTRESPVPDSTKAAYLK
                     AGTDQIVVTTKLDGIPQRMVRTRLMDRIEKSGSLGMWLRAFEAGAAMKRQTGASWLHF
                     IKAARGMTGHGDVPLKQAMMAATMPMLIQKAVVEGDIEHGVMATGVVGGRIAEVPSCQ
                     ELVDRIMAEAEQRLIALGVPKADPGSNVAAASAA"
     gene            complement(2183..3079)
                     /locus_tag="VN627_02155"
     CDS             complement(2183..3079)
                     /locus_tag="VN627_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXD02089.1"
                     /translation="MTDLVEPRKVDIVYETEEPVRYEVIDNVAWITMNRPQFNNAQNG
                     QMTYALDDAFNRAVQDDAVRCIVLAGEGKNFSAGHDIGTPGRDVHKDFENRLMIPGHV
                     NKPAAELLYTREQEQYLGMCRRWRDVPKPTVAMVQGACIAGGLMLAWVCDLIIASDDA
                     FFQDPVNRMGIPGVEYFAHAFELPPRIAKGFILLGDRMSAQKAEQFGMVNRIVTRENL
                     RAETAEVAARLASQPRLGNWLTKQAINHVEELRGKRTAMDAAFHMHHFAHAQNDLVQG
                     SAIAGLGAREMAAANKREAGES"
BASE COUNT          488 a         1103 c         1048 g          598 t
ORIGIN      
        1 gccgaattcc atcagcgtgg ggccgagcag gaaaatcccg ttctggttga cccgcccggg
       61 ggcgccggcg cgatagtatt cttcctcgaa gatcagccac tgaatcaggt ccagcccgcg
      121 cccgccatat tccttcggcc aggtcaccat gccccagtcg ccgctcttca gcgtcgcttc
      181 ccactggcgg tgcctcgaac cccgcgcgcg tcgcgtcgta gtgttccagc ggctccttgg
      241 gcacgtgcgc ctccagccag gagcgtactt cggcgcggaa ggcaatctgg tccggggtat
      301 aggtgaggtc catcagaact tcgccgcgcc cttgccctcg acgaacgcgt cccgtgcctt
      361 ctggctgtcc tcgtgggcgt acatctcgag ggtgaagccc tgctcgatgc ggtacatgtc
      421 gctggggttc cagggctcga tcaggttcag cgcctgcttg gcgatgacca gcgccgtgcg
      481 gctcttgccc gcgatgatct cgcacagggc ccgggcctcg gcctcgagct ggtcgagcgg
      541 gacgatcttc tcgattgaac cgtggcgata gaggtcttca accggcaccc gcccgccggt
      601 gaagaaattg gtgcgcacct tctggaccgg cagcatccgg ctcatgaagg ccgcgccgcc
      661 catggcaccg cgatcgatct cgggcagcga gaagaacgcg ccctcggcgg cgatcaggct
      721 gtcgcaggcg ccggcgatgt tgacgccgcc gccgatgatg aacccatgca gcgcgccgac
      781 caccgggatc gggcaatcgc ggatcgcctt gaaggtcagg taattgtcgc ggttgagctt
      841 ggcgatgcgt tccgggtggg cctgcatttc cttgatatcc acgccgccgc agaagccgcg
      901 gccgttggcc cggatcagca gcacgcgcac gtcctcgtcg cggcccgcct gctcgatcat
      961 ggcggggatg gattcccagc ccttggtgtc gaaggcgttc accggagggt gatcgaacag
     1021 gatctcggcg atgcggtccc ggaccgtcag cgtgatgggc atggtcgtgc ttcctctcag
     1081 gccgcgctgg ccgctgcgac gttggaaccc gggtcggcct tgggcacgcc aagcgcaatc
     1141 aggcgctgtt cggcttcggc catgatgcga tcgacgagtt cctggcagct cgggacttcg
     1201 gcgatccgcc cgcccaccac gccggtcgcc atcaccccgt gctcgatgtc cccttcaacc
     1261 acggccttct ggatcagcat gggcatggtc gcggccatca tcgcctgctt gagcggtacg
     1321 tcgccgtggc ccgtcatgcc ccgcgcggcc ttgatgaagt gcagccacga tgccccggtc
     1381 tggcgcttca tcgccgcccc ggcctcgaac gcgcgcagcc acatgccgag cgaaccggac
     1441 ttttcgatcc ggtccatcaa ccgggtgcgg accatccgct ggggaatgcc gtcaagcttg
     1501 gtggtgacca cgatctggtc ggtcccggcc ttgagatagg cggccttcgt gctgtcgggc
     1561 acggggcttt cccgggtcag caggaagcgc gtgcccatgg cgatgcccac ggctccgtaa
     1621 gccagggccg cgacgagccc acggccatcg gcaaagccgc cgctggcgat caccggcacc
     1681 ttaaccgcat ccagcacttg cggcagcagg atcgtggtcg gcaccgaacc ggtgtggccg
     1741 ccgccttcgc cgccctggac gttgaccatg tcgacgccca gctcttccat tttcctcgcg
     1801 tgcttcagcg cgcccacggt ggggatgcac agaatgcccg catccttgaa ccggccgatc
     1861 atcttggcgt tcggaccgcg cccgaagctg accgcccgga cctgctcggc attggcgagg
     1921 atcaggtcga cgatctgctc ggcccccggc tggaacgagt ggaagttgac cccgaacggc
     1981 ttgtcggtgc ccttgcgcac ggcgaggatc ttttcccggc attcgtcagg cgtcatcacc
     2041 gccgcgccga gaaacccgaa ggccccggca ttactgctgc cgatgacgag gctcggttcg
     2101 gccacccagc ccatcgcggt ctggatgatc ggggcgcggc agcccagccg ctcggtcagg
     2161 acggtttgga gcgcgctcgt catcagcttt cgcccgcctc ccgcttattc gccgccgcca
     2221 tttccctggc gcccagtccg gcaatggcgc tgccctgcac cagatcattt tgcgcgtgcg
     2281 cgaagtggtg catgtggaac gccgcatcca tcgcggtgcg cttgccgcgc aattcctcga
     2341 cgtggttgat cgcctgcttg gtcagccagt tgcccaagcg cggctgggag gccagcctgg
     2401 cggcgacctc ggccgtttcg gcccgcaagt tctcgcgcgt gacgatccgg ttgaccatgc
     2461 cgaactgttc ggccttctgc gcgctcatgc gatcgcccag caggataaag cccttggcga
     2521 tgcgcggcgg cagctcgaag gcatgcgcaa agtactcgac cccggggatg cccatgcggt
     2581 tcaccgggtc ctggaagaag gcatcgtcag aggcgatgat caggtcgcag acccaggcca
     2641 gcatcagccc gccggcaatg caggcgccct ggaccatggc cacggtcggc ttgggtacgt
     2701 cgcgccagcg ccggcacatg ccgaggtact gctcctgctc gcgcgtgtag agcagttcgg
     2761 ccgccggctt gttgacgtgg ccggggatca tcagccggtt ctcgaagtcc ttgtggacgt
     2821 cgcggccggg cgtgccgatg tcgtgcccgg ccgagaagtt cttgccctcg ccggcgagga
     2881 cgatgcaccg cacggcgtca tcctgcacgg cgcgattgaa cgcatcgtcg agcgcgtagg
     2941 tcatctgccc gttctgggca ttgttgaact gcggccggtt catggtgatc caggccacgt
     3001 tgtcgatcac ttcgtaacgg acgggttctt ccgtctcgta gacgatgtcg accttcctgg
     3061 gctcaacgag gtccgtcatc accagctctc ctcagctccg gacgcccgga ggattgtcct
     3121 tgaatacagt ggcccgaacg ttattcgggt caaaccgggc gatcagcgcc agctgctcgg
     3181 ccgtcggcgc gggagtcgtc gggatctctt ccgcgatcct ggcgagcgga aaaccgg
//
LOCUS       DATNBV010000092         4133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4200713,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000092 DATNBV010000000
VERSION     DATNBV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4200713"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VN627_02160"
     CDS             <1..545
                     /locus_tag="VN627_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02090.1"
                     /translation="EGDQVRFFYVRPSVVGVRLDRPANVVGDGRSTIAELVAAKNEER
                     RRRASPGACPVLLDREAHRYLAQQGLDLDSRPAAGKRIFLRGTSNPTTGADTVDCRRI
                     LHPSYARLVEDFCGRLPDMRLAAVDMMMRDASQAAAPGNHWVLEVNGAPGMTSFYFPW
                     EGEPQDVAGALIERLMVEQW"
     gene            626..943
                     /locus_tag="VN627_02165"
     CDS             626..943
                     /locus_tag="VN627_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02091.1"
                     /translation="MRRPLLSAIALLLLASCANPTAETADWRREGTAPEDARADLRAC
                     TLSARKQVDREQANEGMAGDTTSQDSLIGNLNAYSQDKRVDALTRQCMLLHGYQPLTP
                     GAS"
     gene            943..1713
                     /locus_tag="VN627_02170"
     CDS             943..1713
                     /locus_tag="VN627_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HXD02092.1"
                     /translation="MAAKEEEKIVTAAVLIIGNEILSGRTKDANLGYIAEALTKLGIR
                     LREARVVSDVEAEIIAAVNECRARYDYVFTTGGIGPTHDDITSAAVARAFGVPLILDP
                     RARALLEPHYPPGGLNEARLRMAHVPEGADLVLNPVSAAPGFRMGNVFVLAGVPAVMR
                     AMFDGLKGNLRGGPPVLSATVGVYLGEGVIAAGLGALQGRYPDLEIGSYPFFRQGRYG
                     ASFVLRGTDRGLIDTAAVELRALIRQLGAEPIEGEAPG"
     gene            1804..3219
                     /locus_tag="VN627_02175"
     CDS             1804..3219
                     /locus_tag="VN627_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HXD02093.1"
                     /translation="MNWRRIAARLEGPWSPGRYWLLAGSALLALLCWLPHPYNGEEPV
                     YAITTIETWWHGSWLDPMQLGAQYGRPPLLNWVVMVGVWLLGWDHILLAMRLIALAST
                     LGMAAMVAWFAHYLSRDRLVSAFAVACFLTGDLLTQRGWMAYADPLLALATFAAIICL
                     WVAVDRRSPGLLVLAGIAITAAFLTKALTAYAFYGAAGLILLWRHENRRFLISPPSLI
                     IHACIIGFAPLWIFADAGWGQNARLIGDITARAHTGGLGRYILSLLTYWGEMLLRLLP
                     PSALWLALIIRPGLRSSEDPCPKWSLTLRWILLLAALPYLVTTDNHARHLLPLYPLFA
                     IVVAELALRRGRRLLAPLHAALIAWIVVTIPVALVISPWAQIRQHGNSAEVAALIDGQ
                     TRGHPIYTGDGSSETLAVIAHLDVLRLPAEPIGTGPRNIENGFFLYQAPPPEKAGKEV
                     ARYQLGKMSLALLCRGTACKP"
     gene            3284..3372
                     /locus_tag="VN627_02180"
     tRNA            3284..3372
                     /locus_tag="VN627_02180"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3318..3320,aa:Leu,seq:taa)
     gene            complement(3607..>4133)
                     /locus_tag="VN627_02185"
     CDS             complement(3607..>4133)
                     /locus_tag="VN627_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02094.1"
                     /translation="IHWTYDDTSYTPIQDISAAGGSGQLLTQILGNPFNVDQEQFDNA
                     VTDAMYGAHFGPPTHFTTGPAGDFKRIFYVRLGFGGPNPLSINTICTIPPAPPERNTS
                     ANGSVSLSAAFCQEGRVLTYLEASGSGYTGPNDPRFAEFIKNVTFNLFPPNNPNNPNF
                     RDGQPGGCHRFMTC"
BASE COUNT          617 a         1389 c         1382 g          745 t
ORIGIN      
        1 tggaggggga ccaggtccgc ttcttctatg tccggccctc ggtcgtcggc gtccgcctcg
       61 accggccggc caatgtggtg ggagacggca ggtcaaccat cgccgagctg gtcgccgcca
      121 agaacgagga gcgtcggcgc cgcgcgtcgc cgggcgcctg tccggtcttg ctggaccggg
      181 aagcgcatcg ctatctcgcc cagcagggtc ttgacctgga cagccggccg gccgccggca
      241 agcgcatctt ccttcgcggc acctcgaacc ccaccaccgg tgccgatacc gtggattgcc
      301 gccgcatcct gcacccctcc tatgcccggc ttgtggagga tttctgcggc cgcctgccgg
      361 atatgcgcct ggccgccgtc gatatgatga tgcgcgacgc gtcgcaggcg gccgccccgg
      421 gaaaccattg ggtgctggag gtcaacggcg cgccgggcat gaccagcttc tatttcccct
      481 gggagggcga accccaggat gtcgccggcg ccctgatcga gcggctgatg gtcgagcaat
      541 ggtaggattc cccggttgca accgcgggac gggcgacgca gagtaccgcg gtcccgccat
      601 gccgcgatcc cgcttcgagc ctcccatgcg ccgtcctctt ctgtcagcga ttgccctgct
      661 gctgctcgct tcctgcgcca atcccacggc ggagacggcg gattggcgcc gcgagggcac
      721 ggcgccggag gatgcgcgcg ccgatctgcg cgcctgcacg ctctccgcgc gcaaacaggt
      781 cgaccgcgag caggcgaatg agggcatggc cggcgacacg acgagccagg acagccttat
      841 cggcaatctg aacgcctata gccaggacaa gcgggtcgat gccctgaccc ggcaatgcat
      901 gctgctgcac ggctatcagc ccctgacccc aggggcgagc tgatggcggc gaaagaggaa
      961 gaaaagatcg tcaccgccgc ggtgctgatc atcggcaatg agatcctctc cggccgcacc
     1021 aaggatgcga acctcggcta tatcgccgag gcgctgacca agctcggcat ccggctgcga
     1081 gaggcgcggg tcgtctcgga tgtcgaggcg gagatcatcg cggcggtcaa cgaatgccgg
     1141 gcgcgctacg actatgtctt caccaccggc ggcatcggcc cgacccatga cgacatcacc
     1201 tcggccgccg tcgcccgcgc cttcggcgtg ccgctcatcc tcgatccccg ggcgcgcgca
     1261 ctgctggagc cgcattaccc gccgggcggg ctgaacgagg cgcggctgcg catggcccat
     1321 gtgccggagg gcgccgatct ggtcttgaat ccagtctccg cagcaccagg ctttcgcatg
     1381 ggcaatgtct tcgttctggc cggcgtgccg gcggtgatgc gcgccatgtt cgacgggctg
     1441 aagggaaatc tccgcggcgg cccgccggtg ctctcggcga cggtcggcgt ctatctcggc
     1501 gaaggagtca tcgccgccgg gctgggggcg ctgcaggggc gatatcccga tctggagatc
     1561 ggcagctatc ccttttttcg ccagggccgc tatggcgcga gcttcgtgtt gcgcggcacc
     1621 gatcgcggct tgatcgatac cgccgcggtg gagctccgcg ccctgatccg ccagctcggc
     1681 gccgagccga tcgagggcga ggcgccgggt tagagccgca ggcttggtct cacccacggt
     1741 actagacagt ggttcaaatg atcaggccgc ctcgcggcgc cggatagtct ggagagagca
     1801 ggaatgaatt ggcgtcgcat cgccgcgcgg ctcgagggtc cctggtcgcc ggggcgctat
     1861 tggctgctcg ccggttccgc tttgctggcg ctgctctgct ggctgccgca tccctacaac
     1921 ggcgaggagc cggtctatgc catcaccacc atcgagacct ggtggcatgg cagctggctc
     1981 gatccgatgc agctcggcgc ccagtatggc cggccgccgc tcctgaactg ggtggtgatg
     2041 gtcggggtct ggctcctggg ctgggaccat atcctgctgg ccatgcggct gatcgccttg
     2101 gcctcgaccc tgggcatggc agcgatggtc gcctggttcg cccattacct gtcgcgcgac
     2161 cggctggtca gcgcctttgc ggtcgcctgc ttcctcaccg gcgatctgct gacccagcgc
     2221 ggctggatgg cctatgccga tccgctcttg gccctggcga ccttcgccgc catcatctgc
     2281 ctctgggtcg ccgtcgatcg ccgcagcccg gggctcctcg tgctggccgg catcgccatc
     2341 acggccgcct tcctcaccaa ggcgctgacc gcctatgcct tctacggcgc cgccgggctg
     2401 atcctgctct ggcgccatga gaaccggcgc ttcctgatct ccccgccgag cctcatcatc
     2461 catgcctgca tcatcggctt tgccccgctc tggatcttcg ccgatgccgg ctggggccag
     2521 aacgcgcggc tgatcggcga tatcacggcg cgtgcccata ccggcgggct aggccgctat
     2581 atcctgagcc tgctcaccta ttggggcgag atgctgctgc ggctgctgcc gcccagcgcg
     2641 ctgtggctgg cgctgatcat ccgcccgggc ctgcgcagca gcgaggatcc ctgtcccaaa
     2701 tggagtctca cgctgcgctg gatcctgctg ctggccgcac tgccctatct cgtgaccacc
     2761 gacaaccatg cgcgccacct cttgccgctc tatccgcttt tcgccatcgt ggtcgccgag
     2821 ctggcgctca ggcgcggcag gcgcctgctg gcgccgctcc atgccgcgct gatcgcctgg
     2881 atcgtcgtca cgatcccggt cgccctcgtg atcagcccct gggcccagat ccgccagcac
     2941 ggcaattcgg cagaggtggc cgccctgatc gatggccaga ccaggggcca cccgatctat
     3001 accggcgacg gcagcagcga gaccctggcc gtcatcgccc atctcgacgt gctgcgcctg
     3061 ccggccgaac ccatcggcac cggcccgcgg aatatcgaga acggcttctt cctctatcag
     3121 gccccgccgc cggagaaggc aggcaaggaa gtggcgcgat atcagctggg aaagatgagc
     3181 ctggcgctgc tctgccgggg cacggcctgc aagccctgag ggcaggggcg gcgctggccc
     3241 ggcggaggcc gatgttctag tctcccgttc tagtctcccc agggcccgct tggtgaaact
     3301 ggtagacaca agggacttaa aatcccttgg ccggcaacgg ccgtgccggt tcgagtccgg
     3361 cagcgggcac caattttcaa tggctcagca tggttgccac acgtgagatg gctctgtgtt
     3421 cgattgcata gggggtagcg cggagaggca agacggcatc ctgcatcgat gtcccccaac
     3481 cggtccttgt cgggtggctt ctgacgtcgg gggccccagc acttgttccg cggttcgctc
     3541 tgacagcagc gctcacgcac tcccagtatc catacgtcgg tcgcgcgacc gtctgcagct
     3601 aggggcctag caagtcatga accggtggca gccgcctggc tgaccgtcgc ggaaattcgg
     3661 gttgttcggg ttgttcggcg gaaacaggtt gaaggtcacg ttcttgataa actcggcgaa
     3721 gcgcgggtcg tttgggcctg tgtatcccga gccgctggcc tcgagatagg taaggacccg
     3781 gccctcctgg cagaaggccg ccgagaggct gacgcttcca ttggccgaag tgttgcgctc
     3841 cggcggagcg ggcgggatgg tgcatatggt gttgatgctg agcgggttgg gtccgccaaa
     3901 gcccaggcgc acgtagaaaa tgcgcttgaa gtccccggcc ggccctgtgg tgaagtgggt
     3961 cggcggcccg aaatgggcgc catacatggc atcggtgacc gcgttgtcga actgctcctg
     4021 atcgacattg aaggggtttc ccaggatctg ggtcagaagc tggcccgagc cgccggctgc
     4081 ggagatgtcc tggatcggtg tgtaggaggt gtcgtcgtag gtccaatgta tcg
//
LOCUS       DATNBV010000093         5571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4535027,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000093 DATNBV010000000
VERSION     DATNBV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4535027"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VN627_02190"
     CDS             complement(<1..266)
                     /locus_tag="VN627_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXD02095.1"
                     /translation="MPDTAFPLDAATLLARAEAAMGAPDAEHDRIAPNLAALLESVHA
                     EARLSQMGRELTSELIETLLCHRISAQDWVRRHPEVLDEKIEAPV"
     gene            complement(388..2415)
                     /locus_tag="VN627_02195"
     CDS             complement(388..2415)
                     /locus_tag="VN627_02195"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXD02096.1"
                     /translation="MRLLPLAALLAGTAIAAPAHVAPVSVDAEMGPVLANPASAATIK
                     ARCDRWMAGIAARQSALEAERGPATTARTLQRYDDIANLIASASGEFGMLREVMADDA
                     RRSAAAACEVRAANANTRLTLSRKVYDRLKAIPKPRDPATALYLSRTLQTFERSGVAL
                     PASQRAKAQQLQDRIAEVGTAFDKAIADGRKTVMADPAELDGLPADFIAAHQPGADGK
                     VVISTDYPDLFPVLAYAKSESLRRRLYEANVTRAYPANDARLRDLLNLRDEFARLLGR
                     KDHASLALEDKMVDTPAKVETLLTEMASAARPASDRDYARKVAAYQLDHPGAPAFNAW
                     DNAYLTNLVQKRDFAYDRQEARQYFAYDQVRDGILALTSDLFGVTIRPWRTTAWNPQV
                     EAYEMLDRSGPRAGKVIGRFWFDSHPRPGKYNHANMINIRQGVAGKAIPVGVLVMNLP
                     AGDHRTGLMEHGDVETFLHEFGHMLHHIFGGQTARWAGQSGVATEWDFVEAPSQMLEE
                     WVYDYDTLAKFARNAKGEVIPRALVEKMNRARYFDQGMADMRQLALSNISLRYHQGPA
                     PADLGARMRELDAVYNPLPAPPFSQFQDSFGHLNGYSAIYYTYRWSKVIADDLFTRFA
                     KEGLRNPATARDYRRKVLEPGGSKPAAMLVADFLRRPISLDAYKAEMAKDR"
     gene            2479..4833
                     /gene="rnr"
                     /locus_tag="VN627_02200"
     CDS             2479..4833
                     /gene="rnr"
                     /locus_tag="VN627_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HXD02097.1"
                     /translation="MPKEHHKKPKAAGSGTGRHAPGLPSRKQVLDFIAQSDEPAGKRE
                     IAKAFGLKGQEKIQLKALLRDMAEEGLIDGNRTAFHRMGGVPRVTVLRVMEIQDGEAW
                     AIPDSWAPDDATPPPRLRVIEKGRKEAALKIGDRVLARTEELGEEGSGRWLAHPMKKL
                     ATQDEQMLGVVELDATGKGWLAPVDKRVRNAVPISDLGGAEAGQLVMAEPAGRSPRAG
                     VKVTAVLGDPLAPRAFSLIAIHKHGIPHVFSREVLEEGEVAAALALSEESREDLRHLP
                     IVAIDPSDARDHDDAIWAEPDGNGGFNAVVAIADVSFYVRPGSALDREARKRGNSVYF
                     PDRVVPMLPEVLSADVCSLRSGEDRAAMACHMRIDEHGRVQDFRFTRALVRIAEVVAY
                     EEAQRRIDAADGVSTSPDTNGGAVNISVLQNLWAAWRLLEKARGARDPLELELPERRV
                     QLDAAGRITEIAVRERLDAHRVVEDFMIAANVAAAKALESKVAPVVYRIHEPPSREKL
                     VALKDYLATFGRKLALGQVITPGLFNRMLKDVSDEAEKALVMEAVLRSQTQAYYGPRN
                     AGHFGLALGSYAHFTSPIRRYSDLLVHRALVDAFGLEQPVKGAKGSQGVPAHSGLSER
                     DRADLSRVSDAISQAERRAMEAERDTIDRYVAAWLSVRVGEVFPCRITGVRKFGFFAT
                     IVGLGGDGLVPVSTLGDERFHYDEKAQVLVGEESGEAYAIGKMLDLRLAEANPLTGAL
                     KFELPESEGRVERRGRPMELKKRGGHLVGRRGRPANIRHQGRRK"
     gene            complement(4885..5340)
                     /locus_tag="VN627_02205"
     CDS             complement(4885..5340)
                     /locus_tag="VN627_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXD02098.1"
                     /translation="MRTYLVIIDETVEAGTALRFASRRAAKTGGQLQLLALVPRQQFV
                     AFGGVQATFEEEARARAEVMVTSLAGNVFTQSGKMPAIAVCQGEAVKVVTDYLAQHPE
                     VAALVLGAAPEGSPGPLVSWFSGAGLGSLPCPLYIVPAALDDSRLDDLS"
     gene            5463..>5571
                     /locus_tag="VN627_02210"
     CDS             5463..>5571
                     /locus_tag="VN627_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HXD02099.1"
                     /translation="MPIEIKMPALSPTMEEGTLSKWLVKEGDSVASGDVL"
BASE COUNT          884 a         1715 c         2065 g          907 t
ORIGIN      
        1 actggcgcct cgatcttctc gtccagcact tcgggatggc gccgcaccca gtcctgcgcc
       61 gagatgcggt ggcacagcag ggtttcgatc agttccgagg tgagctcccg ccccatctgg
      121 ctcagccgcg cctcggcgtg aaccgattcg agcagcgcag cgagattggg tgcgatgcga
      181 tcatgctcgg catcgggcgc gcccatggct gcctcggcac gggcgaggag cgtggccgca
      241 tctagcggaa aggcggtatc cggcatctgt atctcccggc ccctcgaccg gggcgcttgt
      301 ccttgaatcg aaaggttcgg cacaagggga ggagtgtcaa gcgagcgccc ggcttcgtcg
      361 tacctcatcg cctatctgtt gggcggctta ccggtccttc gccatctccg ccttgtaggc
      421 atccaggctg atcggcctcc gcaagaaatc ggcgacgagc atcgccgccg gcttgctccc
      481 gccgggctcc agcaccttgc ggcggtagtc ccgcgccgtt gccggattac gcaggccttc
      541 cttggcaaag cgggtgaaca ggtcatccgc gatgaccttg gaccagcggt aggtgtagta
      601 gatcgccgag tagccgttga gatggccgaa gctgtcctgg aactggctga atggcggggc
      661 agggagcggg ttgtaaaccg cgtccagctc gcgcatccgc gcgccgagat cagccggcgc
      721 agggccctgg tggtagcgca gcgagatgtt ggacagggcg agctggcgca tatccgccat
      781 gccctgatcg aaatagcggg cgcggttcat cttttcgacc agggcgcggg ggatgacctc
      841 gcccttcgcg ttgcgggcga acttggcgag cgtatcgtaa tcgtagaccc attcctccag
      901 catctggctg ggcgcctcga caaagtccca ctcggtggcg acgccgctct gcccggccca
      961 ccgcgcggtc tgcccgccga agatgtggtg cagcatatgg ccgaattcgt ggaggaaagt
     1021 ctcgacgtcg ccgtgttcca tgaggccggt gcggtgatcg ccggcgggca ggttcatgac
     1081 gaggacgcca acggggattg ccttgccggc cacgccctgc ctgatgttga tcatgttggc
     1141 atggttgtac ttgcccgggc gcgggtggct gtcgaaccag aaccggccga tgaccttgcc
     1201 ggcgcgcggg cctgagcggt cgagcatctc gtaggcttcc acttgcggat tccatgccgt
     1261 ggttcgccac gggcggatgg taacgccgaa caggtcggac gtgagcgcca ggatgccgtc
     1321 gcgcacctga tcgtaagcga agtattgccg ggcttcctgc cggtcgtagg cgaaatcccg
     1381 cttctggacg aggttggtca ggtaggcgtt gtcccaggca ttgaacgcgg gcgcaccggg
     1441 atggtcgagc tggtaggccg ccaccttgcg ggcatagtcg cggtcgctgg cgggcctggc
     1501 cgccgaggcc atttccgtga ggagggtttc gaccttggcc ggcgtatcga ccatcttgtc
     1561 ctcgagcgcg agactggcgt ggtccttgcg gccgagcagg cgggcgaact cgtcgcgcaa
     1621 gttgagcagg tcgcgcaggc gggcatcgtt ggccgggtag gcgcgggtga cattggcctc
     1681 gtagagccgg cggcgcaggc tttcgctttt ggcataggcc aggaccggaa acaggtcggg
     1741 atagtcggtc gagatgacga ccttgccatc ggccccgggc tggtgggcgg cgatgaaatc
     1801 agccgggagc ccgtcgagct cggcgggatc ggccatgacg gtcttgcggc catcggcaat
     1861 cgccttgtcg aaggcggtgc cgacttcggc gatgcggtcc tgcaactgct gcgccttggc
     1921 gcgctgggat gccggcaggg caacgcccga gcgttcgaag gtctgcaggg tgcgcgagag
     1981 gtaaagcgcg gtggcgggat cccggggctt cgggatggcc ttcagccggt cgtagacctt
     2041 gcgcgacaag gtgagccggg tgttggcatt ggcggcgcgg acttcgcagg cggcggcggc
     2101 ggagcggcgg gcatcgtcag ccatgacctc gcgcagcatg ccgaattcgc ctgatgccga
     2161 ggcgatgagg ttggcgatgt cgtcatagcg ctggagagtg cgggcggtgg tggcggggcc
     2221 gcgttcggcc tccagcgcgg actggcgggc ggcgatgccc gccatccagc ggtcgcaccg
     2281 cgccttgatc gttgccgcac tggcgggatt ggcgaggacc ggccccattt ccgcgtcgac
     2341 cgagacgggg gcgacatggg cgggggccgc aatggcggtg ccggcgagca gggcggcgag
     2401 gggcaggagg cgcatgaccg gaactctcgt gagcggcgcg cgattgtcaa tcgtccggat
     2461 tgcggccaag gcgggaatat gcccaaggaa catcacaaga agcccaaggc cgccggatct
     2521 ggaactgggc gccacgcgcc cggcttgccg agccgcaagc aggtgctcga cttcatcgcc
     2581 cagtccgacg agcccgccgg caagcgcgag atcgccaagg ccttcgggct caaggggcag
     2641 gagaaaatcc agctcaaggc cctgctgcgc gacatggccg aggaaggcct gatcgacggc
     2701 aaccgtaccg ccttccaccg catgggcggc gtgccgcggg tcaccgtgct gcgggtgatg
     2761 gagatccagg acggcgaggc ctgggcgatc ccggatagct gggctccgga tgacgccacg
     2821 ccgccgccgc gcctcagggt gatcgagaag ggccgcaagg aagccgcgct caagatcggc
     2881 gaccgggttc tggcccgcac cgaggaactg ggcgaggagg gttcgggccg ctggctcgcc
     2941 catccgatga agaagctggc gacgcaggac gagcagatgc tcggcgtggt cgagctggac
     3001 gccacgggca agggctggct ggcgccggtg gacaagcgcg tgcgcaatgc cgttccgatt
     3061 tcggaccttg gtggcgcgga ggccgggcag ctggtcatgg ccgagccggc ggggcggagc
     3121 cctcgggcgg gggtcaaggt gacggcggtg ctgggcgatc cgctggcccc gcgcgccttc
     3181 agcctgattg ccatacacaa gcacggcatc ccgcacgtgt tcagccgcga ggttctggag
     3241 gagggcgaag ttgccgccgc cctggcgctt tcggaagaga gccgcgagga cctgcgccac
     3301 ctgccgatcg tcgcgatcga tccgagcgac gcgcgcgacc acgacgatgc gatctgggcc
     3361 gagccggatg gcaatggcgg gttcaatgcg gtagtcgcca ttgccgatgt cagcttctac
     3421 gtgcggcccg gttcggcgct ggaccgcgag gcgcgcaagc ggggcaattc ggtctatttc
     3481 cccgaccggg tcgtgccgat gctgccggaa gtcctttcgg ccgacgtctg ctcgctgcgc
     3541 agcggcgagg accgcgccgc catggcctgc cacatgcgga tcgacgagca cggccgggtg
     3601 caggacttcc gcttcacccg cgcgctggtg cggattgccg aggtggtggc ctatgaggag
     3661 gcccagcggc ggatcgacgc ggcggatggc gtttcgactt cgcccgacac gaacgggggt
     3721 gcggtaaaca tctctgtact ccagaacctg tgggcggcct ggcggctgct ggaaaaggcg
     3781 cggggcgcgc gcgatccgct ggagctggaa ttgccggagc ggcgggtcca gctcgatgcc
     3841 gcggggcgga tcaccgagat cgccgtgcgc gagcggctgg acgcgcaccg ggtcgtggaa
     3901 gacttcatga tcgccgccaa cgtcgccgcg gccaaggcgc tggaaagcaa ggtggcgccg
     3961 gtggtctacc gcatccacga gccgccgagc cgggaaaagc tcgtcgcgct caaggactac
     4021 ctcgcgacgt tcgggcgcaa gctggcgctg gggcaagtga tcacgccggg gctgttcaac
     4081 cgcatgctca aggacgtgag cgacgaggcg gaaaaggcgc tggtgatgga ggccgtgctg
     4141 cgcagccaga cccaagccta ttatggtccg cgcaatgccg ggcacttcgg cctcgcgctc
     4201 gggtcctatg cccacttcac ttcgccgatc cggcgctatt cggacctgct ggtccaccgc
     4261 gcgctggtcg acgcgttcgg gctggagcag ccggtgaagg gcgccaaggg cagtcagggc
     4321 gtgccggccc attcgggtct ttcggagcgc gaccgggcgg acttgtcgcg cgtttcggat
     4381 gcgatcagcc aggccgagcg gcgggcgatg gaggccgagc gggatacgat cgaccgctat
     4441 gtcgccgcct ggctgtcagt acgggtgggc gaggtgtttc cctgccggat caccggggtg
     4501 cgcaagttcg gcttctttgc gaccatcgtc ggcctgggcg gcgacgggct ggtgccggtc
     4561 tccacgctgg gcgacgagcg gttccactat gacgagaagg cgcaggtgct ggttggcgag
     4621 gagagcggcg aggcctatgc catcggcaag atgctcgatc tgcggctggc cgaggccaat
     4681 ccgctgaccg gcgcgctcaa gttcgagctg cccgagagcg agggccgggt ggagcggcgc
     4741 gggcggccga tggagctgaa gaagcgcggt ggccatctgg tgggacggcg cgggcggccg
     4801 gcgaatatta ggcaccaagg gcggcgaaag tagcgccggg ctgcagggac gacggcgcta
     4861 tcgtgtgctg ctgccccgga aacgtcagct caggtcatcc agccggctgt cgtcaagcgc
     4921 ggccggtacg atgtagagcg ggcaggggag ggagccgagc cccgcgccgg agaaccagct
     4981 gacgagcggc cccgggctcc cttccggcgc cgcgccaagg acgagggcgg cgacttcggg
     5041 gtgctgggcg aggtagtcgg tcaccacctt gaccgcttcg ccctggcaca cggcgatggc
     5101 cggcatcttg ccgctctgcg tgaagacatt cccggccagc gaggtgacca tcacctcggc
     5161 ccgtgcgcgg gcctcttcct cgaaggtggc ctggacgccg ccgaaggcga cgaactgctg
     5221 gcgcgggacg agggcaagga gctggagctg gccgccggtc ttcgcggcgc ggcgcgaggc
     5281 gaagcgcagg gcggtcccag cctcgacagt ctcatcaatg ataaccagat aggttctcat
     5341 taataccatc cttcgaattg atgtgccgtt ttggcaaaat ttgctttatc ggtcttgcga
     5401 taacttcggg tgcggttcac caccgcgcct cggagacgac gcctgccgga gcacgccctg
     5461 ccatgccgat cgaaatcaag atgccagccc tctcccccac catggaagag ggtacgctat
     5521 ccaagtggct ggtcaaggag ggagatagcg ttgcctccgg cgatgtcctc g
//
LOCUS       DATNBV010000094         3056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2282532,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000094 DATNBV010000000
VERSION     DATNBV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2282532"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="VN627_02215"
     CDS             complement(<1..1327)
                     /locus_tag="VN627_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HXD02100.1"
                     /translation="MTNKLTRFAALAGLFALATLPVAAHAQAIPATLAPEALADAADS
                     GDTAWILVSSALVMLMAAPGLTLFYGGLLRAKNFLSVMVQCGAIVAVASLLWILVGYT
                     LAFGPVTNGWIGAGTNAMFNNLGNVRANYTVPESAFALFQMCFAAITPALMAGAWVDR
                     ARFGWVVGFSAPWGLVVYAPVAHWVWGGGWLATKLGTLDFAGGIVVHTTAGVSALVVA
                     ALLGRRNGFPKTLMLPHSPALTMAGAALLWMGWFGFNGGSALAANDDAAAAIINTHAA
                     ACAAALAWLAIERVSVGKPTSVGFATGAVAGLATVTPAAGFISPGAAILFGIAAAAIC
                     YPMIQLIKFRLKIDDSLDVFAVHGVGGMTGSILLALFLAPAVGGTGYAQHMGLASQLV
                     AQVVGVGVVAVFSVIATAVLALAVNLALPMRVSEDEEREGLDLTSHGERA"
     gene            1489..2016
                     /locus_tag="VN627_02220"
     CDS             1489..2016
                     /locus_tag="VN627_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXD02101.1"
                     /translation="MRFRRLFRNIPILAALAVALAVALAGAPARAGDNDVVPYWASLR
                     AKQVNMHVGPGEDYRTSFRYHREDLPMKVLRKMEGWRLVQDPDGSRGWMLARFLKPER
                     GAIVVAAKGGSGAGGDGLAEMHQAPDAGSRLLWRLEPGVTGKLGDCAEGWCKFVVGGS
                     AGYVREDALWGPGQP"
     gene            2171..2557
                     /locus_tag="VN627_02225"
     CDS             2171..2557
                     /locus_tag="VN627_02225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXD02102.1"
                     /translation="MVDHSSYLVQYLPILIFLVIAFALSAAFVFLPMGVARLTGTHNP
                     SADKNAEYECGFPAFEDPRSQFDVRFYLVAILFIIFDLEAAFLFPWAVSLKETGWTGW
                     LTMMIFLAELVIGFAYAWKKGALDWE"
     gene            2554..>3056
                     /locus_tag="VN627_02230"
     CDS             2554..>3056
                     /locus_tag="VN627_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HXD02103.1"
                     /translation="MNDMLAKPASGPMVAPDQAFFDSLNTEVSDKGFLVTSTEELFQW
                     ARTGSLWWMTFGLACCAVEMIHVNMPRYDMERFGVAPRASPRQSDVMIVAGTLCNKMA
                     PALRKVYDQMSDPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEA
                     LVYGIIQL"
BASE COUNT          486 a         1012 c         1069 g          489 t
ORIGIN      
        1 aggcgcgctc gccgtggctg gtgaggtcga ggccctcgcg ttcctcgtcc tcggagaccc
       61 gcatcggcag ggcgaggttg acggcgaggg caagaacggc ggtggcgatg acggagaaca
      121 ccgcgacgac gccgacgccg acgacctggg cgacgagctg gctggcgaga cccatgtgct
      181 gggcatagcc ggtaccgccg accgccgggg cgaggaacag ggccagcagg atcgacccgg
      241 tcatcccgcc gacgccgtgg acggcaaaga cgtcgagcga atcgtcgatc ttgaggcgga
      301 acttgatcag ctggatcatc gggtagcaga tggccgccgc ggcgatcccg aacaggatcg
      361 cggcgcccgg cgagatgaac ccggcggccg gggtgaccgt ggcgaggccc gccaccgcgc
      421 cggtggcgaa gccgaccgag gtgggcttgc cgaccgagac ccgttcgatc gccagccagg
      481 cgagcgcggc ggcgcaggcg gcggcgtggg tgttgatgat cgcggcggcc gcatcgtcgt
      541 tggcggccag cgccgagccg ccgttgaagc cgaaccagcc catccacagc agggccgcgc
      601 cggccatggt cagggcaggg ctgtgcggca gcatcagggt cttgggaaag ccgttgcggc
      661 ggccgagcag cgcggccacg accagggcgg aaacgccggc ggtggtgtgg acgacaatgc
      721 cgccggcgaa gtcgagcgtc ccgagcttgg tggcgagcca gccgccgccc cagacccagt
      781 gcgcaacggg cgcgtagacg accaggcccc acggggcgct gaagccgacc acccagccga
      841 accgcgcccg gtccacccag gcgccggcca tcagcgccgg ggtgatcgcg gcgaagcaca
      901 tctggaacag cgcgaaggcg ctctccggca cggtgtagtt cgcgcggaca ttgccgaggt
      961 tgttgaacat ggcgttcgtg ccggcgccga tccagccgtt ggtcaccggc ccgaaggcga
     1021 gggtatagcc caccaggatc cacagcagcg aggcgaccgc cacgatcgcg ccgcactgga
     1081 ccatgaccga gaggaagttc ttggcccgca gcaggccgcc gtagaacagg gtcagcccgg
     1141 gcgccgccat gagcatgacg agcgccgagg agacgaggat ccaggccgta tcgccgctgt
     1201 cggccgcgtc ggcgagggct tctggggcca gagttgctgg aatcgcctgg gcatgggcgg
     1261 ccacgggcag cgtggcgagc gcgaacaggc ctgccagcgc ggcgaagcgc gtcagtttgt
     1321 tggtcatcga tgtccctccg ggcgccgggt tcccggtcgc gattgccgca tcgcttagga
     1381 cattgccccg aacttgcgca agcttcgcta cattacagtg tggacaggag cgtcaccccg
     1441 ccgtcgaagg cagttgctgc cgccgcactt gcgtggttat ggacgtccat gcgctttcgc
     1501 cgtcttttcc ggaatattcc catcctcgcg gccctcgccg tcgccctcgc cgtcgccctc
     1561 gccggcgcgc cggcgcgggc cggggataac gatgtcgtgc cctattgggc ttccctgcgg
     1621 gccaagcagg taaacatgca cgtcggcccg ggggaggatt accgcacctc gttccgctac
     1681 caccgcgaag acctgccgat gaaggtcctg cgcaagatgg agggctggcg gctcgtccag
     1741 gaccccgatg gctcgcgcgg ctggatgctt gcccgcttcc tcaagccgga acgcggcgcc
     1801 atcgtcgtcg ccgcgaaggg tgggagcggg gccggcggtg atggccttgc cgaaatgcac
     1861 caggcccccg atgccggctc ccgcctgctg tggcgcctcg agccgggcgt gaccggcaag
     1921 ctgggcgact gtgccgaggg ctggtgcaag ttcgtcgtgg ggggcagcgc cggctatgtc
     1981 cgtgaagatg ccctgtgggg ccccggccag ccctgatccc cgggccccga tccgcggtcc
     2041 ggtccacggc ggtactatta gcagtgatca gacatgcgca ttttggcgga gcagaggggt
     2101 tggcccactt gcgcatgccg cactgcggga ctagggcgcc ccggtagttc cgcaaggcga
     2161 gtcagccccc ttggtcgacc attcatccta tctggttcag tacctgccga tcctgatctt
     2221 cctggtcata gcctttgcgc tgtcggcggc cttcgtgttc ctgcccatgg gggtggctcg
     2281 gcttaccggc acgcacaacc ccagtgccga caagaacgcg gagtacgagt gcggtttccc
     2341 cgccttcgag gacccgcgca gccagtttga cgtgcggttt tatctggtcg cgatcctgtt
     2401 catcatcttc gatctcgagg ccgcgttcct gtttccctgg gcggtgagcc tgaaggaaac
     2461 cggctggacc ggctggctga cgatgatgat cttcctcgcc gaactggtga tcggcttcgc
     2521 ctatgcgtgg aagaaggggg cgttggactg ggaatgaacg atatgctcgc caagcccgcc
     2581 tccggcccca tggtcgcgcc cgaccaggcc tttttcgaca gcctgaacac cgaggtgtcc
     2641 gacaagggct tcctcgtcac ctccaccgag gaactgttcc agtgggcccg caccggctcg
     2701 ctgtggtgga tgaccttcgg cctcgcctgc tgcgcggtcg aaatgatcca cgtcaacatg
     2761 ccccgctacg atatggagcg cttcggcgtc gccccgcgcg cgtccccgcg ccagtccgac
     2821 gtgatgatcg tcgccggaac cctgtgcaac aagatggccc cggccctgcg caaggtctac
     2881 gaccagatgt cggacccgaa atacgtgatc tcgatgggtt cgtgcgccaa tggcggcggc
     2941 tattatcact acagctactc ggtggtgcgc gggtgcgatc gcatcgtccc ggtagacgtg
     3001 tacgtgccgg gctgcccgcc gacggcggag gcactggtgt acggcatcat ccagct
//
LOCUS       DATNBV010000095        11885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1646816,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000095 DATNBV010000000
VERSION     DATNBV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11885
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646816"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VN627_02235"
     CDS             <1..181
                     /locus_tag="VN627_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HXD02104.1"
                     /translation="ANIGDTRSLIIHPASTTHSQLGEEELQAAGAGPDTVRVSVGIEH
                     IDDIVADLAQALEQI"
     gene            202..2223
                     /locus_tag="VN627_02240"
     CDS             202..2223
                     /locus_tag="VN627_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HXD02105.1"
                     /translation="MFKKILIANRGEIACRVIKTARRMGIQTVAVYSDADARAPFVQM
                     ADEAVHIGPAPAAQSYLIADKIIAACKQTGAEAVHPGYGFLSERTSFAEALAAEGIEF
                     IGPPVNAIAAMGDKIESKKLAKAAGVNVVPGFVGEIEDTEHAVRISNEIGYPVMMKAS
                     AGGGGKGMRLAYNEKDVREGFEATKREGLNSFGDDRVFIEKFILNPRHIEIQILGDKH
                     GNVLYLNERECSIQRRHQKVVEEAPSPFVTPKMRKAMGEQCVALSKAVGYYSAGTVEL
                     IVSGADPSGESFYFLEMNTRLQVEHPVTEAITGVDLVEQMIRVAAGEKLAMTQDEVKI
                     DGWAIENRVYAEDPYRGFLPSTGRLIRYNPPVPGWTDDGAENGRRGVDGVRVDDGVYE
                     GGEVSMFYDPMIAKLVTWGETRDEAADKQIAALDAFEIEGLGHNIDFVNAIMQHPRFR
                     SGELTTGFIAEEYPEGFTGAATSEELKAKLAAIAGFIATARADRARNVDGQLAGHLPP
                     PFEWQVKLGGSTFAVEVDEDDVVVDGKVIDLAMEYTPGDRIVFAEADDEDIALKVEPT
                     RGGLKLTTRGAIHKVGILPAQVAHLAGHMIEKVPPDLSKFLICPMPGLLVSLNVAEGD
                     KVEAGQPLAVVEAMKMENILRAEKSATVKKVNATAGESLAVDAIILELE"
     gene            2299..2733
                     /locus_tag="VN627_02245"
     CDS             2299..2733
                     /locus_tag="VN627_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HXD02106.1"
                     /translation="MPTIWTIGYEKTTQDALIAALQEAGVEVLADIRALPLSRRPGFS
                     KSSLAAAARAGGMEYRHLKPLGTPAEGRAAARRHDHAALERIYAGQLELPEALAAMAE
                     LRSLASEQKVALLCYEREAAECHRSLLIGALLADFAVIDLKP"
     gene            complement(2756..3538)
                     /locus_tag="VN627_02250"
     CDS             complement(2756..3538)
                     /locus_tag="VN627_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HXD02107.1"
                     /translation="MQLAIHTRLDYGFPEPTAVILQVEAAMIPEQDVSQPWIGLSPVE
                     HFARVPGQDSIGERIMLRVAERLTVDYHATVNVQRITRDLADLPAMPLHLLPGPTVPY
                     LLESRYCPSHSFERFVAGEFPGLSGGALILAMSDYLRANFRYVAGVSTSATTAIDTFV
                     SREGVCRDYAHVLIAMARAAQIPARIASVYAPDVSPPDFHAVCEVFLAGEWHLVDPTG
                     MATGADMAKIGIGRDAADVSFLTSFGYTWLNSQEISVTRETA"
     gene            complement(3587..4702)
                     /gene="aroB"
                     /locus_tag="VN627_02255"
     CDS             complement(3587..4702)
                     /gene="aroB"
                     /locus_tag="VN627_02255"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HXD02108.1"
                     /translation="MAVIPVDVAGRPYEVRIAAGLLARAGEECAPFLRKKRVPVITDA
                     NVHNAWGQTVGASLAAAGFEPVWKILPAGEQTKSWEHLAEVTDWLLAEGVERKDAVIA
                     LGGGVIGDLTGFAAAILKRGCQFIQLPTTLLSQVDSSVGGKTAINTPAGKNLVGAFHQ
                     PALVLADPQALETLPRRELGAGYAEVLKYGLLGDAEFFQWCEANGAALMDGDHAAREH
                     AIARSVEAKARIVEQDEFETTGLRALLNLGHTFGHALEAETGYSARLLHGESVALGMV
                     LAARYSARRGELPAADAERVAAHFRSLGMMSELVELGLNVDGRALVAHMLHDKKMDAG
                     TLPFLLLRGIGGAYLAKDVDLADVAAFLDGQLQGAPA"
     gene            complement(4693..5286)
                     /locus_tag="VN627_02260"
     CDS             complement(4693..5286)
                     /locus_tag="VN627_02260"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HXD02109.1"
                     /translation="MEHDDARLDPADNAALARLISRQLKRPIVLVGMMGVGKSSVGRR
                     LANLLGLAFIDADEAIEQAAQMTIPEIFATYGEDYFRDGERRVIQRLIEESAGKRPMI
                     IATGGGAFVNPETRALILAKAIAVWLDADVETLLQRVGRKDNRPLLRTGNPREILTRL
                     KAEREPAYAQAPVHVQSGNQPHSQTLAAVMKGVAQWL"
     gene            5418..5558
                     /locus_tag="VN627_02265"
     CDS             5418..5558
                     /locus_tag="VN627_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02110.1"
                     /translation="MKQPGRIALVVIVALGLALLAWAWHNGGREPLHEIAVPVAVPES
                     AK"
     gene            5555..7393
                     /locus_tag="VN627_02270"
     CDS             5555..7393
                     /locus_tag="VN627_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02111.1"
                     /translation="MKRALLLAGAALALTSAFVAAQDAPESLLPPGFDRPAPRPARAA
                     PAPAPTGPAAAAPAQGQSSPVIQPLPVAAPRSGAVALPSNLPSLDQLEAMSPDELDKV
                     LDLRPKDDIPPAARRAMTRVGVIDEGEGGLPFWNLGKQDPDLVRAVLQGNSGKLVSRW
                     GHILLRRAVASRLDAPAGMDPAEFAALRAALLVRMGEGDGARSLVQDVDTANYSPELT
                     QAALDAYVATGDFTGICPVIALQGASRKDKPWQVLQAMCDGFGGDTSRSMASLDRLTY
                     YGAMPRIDMLLAQKYAGAAGKARKAVTIEWDKVTDMTPWRYAMTTAVGLQPPAALMKG
                     LPLDYAFQAATAPALALDGRAAAADKAAACGVLSSAAMVDLYGQLFDEDDANGEWAQR
                     AAKLRDAYVAETVGDRAGAIRDLWGDESDPLRRYSRQVLTAYAAARLPVSADLAGEAG
                     PLIAAMLSAGLDGNAMRWAGVVKSGSEGWALLALANPAANPAADQGGIDDFHGNDKSK
                     GSRKSAFLVAGLAGLGRLPVATAASMAEKYGFQLDRQSRWAQAIDRAAGYDNRALVAL
                     LAGLGMQGDSWERMTPRNLYHVVAALNRVGLSAEARMIAAEAVARG"
     gene            7390..8331
                     /locus_tag="VN627_02275"
     CDS             7390..8331
                     /locus_tag="VN627_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HXD02112.1"
                     /translation="MRQARAADPVELFLAMLAAERGAAANTLAAYRRDLEGAEEVLGD
                     LTRADGPALAGLGQAWAGLAPASLARKCSALRQFYGFLVDEGMREDDPSPALPRPRTR
                     RPLPRLLSHEEVERMLGRAEEEAAGGTPAALRMLALLELLYGSGLRASELVSLSLAAV
                     PRDAPFLHVTGKGGQQRLVPVSTRAAQALARWLAVRPGGGTRLFPSSSAQGHLTRVRL
                     FQLLRELATRAGIAAEKVSPHVLRHAFATHLLEGGADLRVLQTLLGHADIATTQIYTH
                     VEAARLVALVNARHPLAGGSAAGTGSADGLARGSAAD"
     gene            8341..9285
                     /locus_tag="VN627_02280"
     CDS             8341..9285
                     /locus_tag="VN627_02280"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HXD02113.1"
                     /translation="MISFLEFEKPVAALEARIVELRATAADGDIDLASEIERLEKKSA
                     ALLASTYQALTPWQKTQVARHPARPHFVNYIAGAFSGFMPLGGDRTFGDDQAIIGGFA
                     MLGHRRVMVIGHEKGHDTQTRIRHNFGMGKPEGYRKAIRLMELAGRFGLPVVTLVDTS
                     GAFPGVEAEERGQAEAIARSTEACLSLPVPMVAAIVGEGGSGGAVALASAERVLMLEH
                     AVYSVISPEGASSILWRTAEKAPDAAEAMKMTAQDLLKLGVIDRIVPEPVGGAHRDPL
                     RTAVSLGAAIGEELDGLAKAKPSDLMKRREERFLAIGG"
     gene            complement(9436..9609)
                     /locus_tag="VN627_02285"
     CDS             complement(9436..9609)
                     /locus_tag="VN627_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HXD02114.1"
                     /translation="MTFFKNLIRDEAGATAIEYGLIAALIAVAVMTTVSGLGSQLNTT
                     FSKTSSQLTAANS"
     gene            complement(9725..9910)
                     /locus_tag="VN627_02290"
     CDS             complement(9725..9910)
                     /locus_tag="VN627_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HXD02115.1"
                     /translation="MNLMITIRQMLRECSGATAIEYGLIAALVVVTVMFTVQGVANET
                     NRMWSNVGSTMAQATAS"
     gene            10088..10480
                     /locus_tag="VN627_02295"
     CDS             10088..10480
                     /locus_tag="VN627_02295"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HXD02116.1"
                     /translation="MLVVAAALIAADGRVCLQQRPEGKNHAGLWEFPGGKVEPGESPE
                     AALVREICEELGLTLEAAELMPCGFAGDAGIVILLYASRAWSGEAGPLEGGALGWFAP
                     EALAGQPMPPLDYPLARQVCKLLAGGMI"
     gene            10527..10603
                     /locus_tag="VN627_02300"
     tRNA            10527..10603
                     /locus_tag="VN627_02300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10561..10563,aa:Arg,seq:acg)
     gene            10833..11360
                     /locus_tag="VN627_02305"
     CDS             10833..11360
                     /locus_tag="VN627_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02117.1"
                     /translation="MTDDDGIRIIASNSEAELGARLALGDLSFNLRELAANMLRVVRG
                     AGRPENLGRQMAEVVALYARYRDQAGAYPSNYDIHQAISLRSEGNARKHTDKWSHAID
                     DIVQGSLQIAASQLLGQSTQEAAGESELMEGVARIEAIREANRLEALERRRSELAFSK
                     SKSGAKRKPVRPRGE"
     gene            11368..11514
                     /locus_tag="VN627_02310"
     CDS             11368..11514
                     /locus_tag="VN627_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02118.1"
                     /translation="MMERSDVDLDRVHQLVATATGDDVQLRARARWFVDWCASKYDTE
                     GERP"
     gene            11567..11878
                     /locus_tag="VN627_02315"
     CDS             11567..11878
                     /locus_tag="VN627_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02119.1"
                     /translation="MVLGYIAFAIYAWFDFASTNHDGLANLGLIVVTAPVAVVGFVLS
                     GGGSFALIPEGRGYLADHAIYYVPAVACTALFLWLIGRRIERAFRSADSTHDDGPPAG
                     P"
BASE COUNT         1942 a         3740 c         4283 g         1920 t
ORIGIN      
        1 cgccaacatc ggcgacaccc gctcgctgat catccacccg gcctcgacga ctcacagcca
       61 gctcggcgag gaagaactgc aagccgccgg agcgggcccg gacaccgtcc gcgtctcggt
      121 cggcatcgaa cacatcgacg acatcgtcgc tgatctggcg caggcgctgg aacagatctg
      181 aaggactgga aagagaagcc catgttcaag aaaatcctga tcgcaaaccg tggcgaaatc
      241 gcttgccgcg tgatcaagac ggcccggcgc atgggcatcc agaccgtggc ggtctattcg
      301 gacgccgacg cccgcgcgcc cttcgtgcag atggctgacg aggccgtgca catcggtccg
      361 gcgcccgccg cgcagtcgta cctgatcgcc gacaagatca tcgcggcctg caagcagacc
      421 ggcgccgagg cggtccaccc gggctacggc ttcctgtccg agcggacctc ctttgccgag
      481 gcgctggccg ccgagggcat cgagttcatc ggccccccgg tgaacgcgat cgccgccatg
      541 ggtgacaaga tcgaatccaa gaagctcgcc aaggccgccg gcgtcaacgt cgtccccggc
      601 ttcgtcggcg agatcgagga taccgagcat gccgtgcgca tctcgaacga gatcggctat
      661 ccggtgatga tgaaggcctc ggccggcggc ggcggcaagg gcatgcgcct cgcctacaac
      721 gagaaggacg tgcgcgaggg ctttgaagct accaagcgcg agggcctgaa ttcgttcggt
      781 gatgaccgcg tgttcattga gaagttcatc ctcaacccgc gccacatcga gatccagatc
      841 ctcggcgaca agcacggcaa cgtgctttac ctcaacgagc gcgaatgctc gatccagcgc
      901 cgccaccaga aggtggtgga agaagcgcct tcgcccttcg ttacgcccaa gatgcgcaag
      961 gccatgggcg aacagtgcgt cgcactgtcg aaggccgtgg gctattacag cgcgggcacg
     1021 gtggaactga tcgtctcggg cgcggacccg agcggcgaga gcttctattt cctcgagatg
     1081 aatacccgcc tgcaggtgga gcatcccgtc accgaggcga tcacgggcgt cgaccttgtc
     1141 gagcagatga tccgtgtggc cgccggcgag aagctggcga tgacccagga cgaagtgaag
     1201 atcgacggct gggccatcga aaaccgcgtc tatgcggagg acccctatcg cgggttcctg
     1261 ccttcgaccg ggcggctgat ccgctataat ccgcccgtgc cgggctggac cgacgacggc
     1321 gcggagaacg ggcggcgcgg ggttgacggc gtgcgcgtgg acgacggcgt ctatgagggc
     1381 ggcgaagtct cgatgttcta cgatccgatg atcgccaagc tcgtcacctg gggcgagacc
     1441 cgcgacgagg cggcggacaa gcagatcgcg gcattggacg cgttcgagat cgaggggctt
     1501 ggccacaaca tcgatttcgt gaacgcgatc atgcagcacc cgcgcttccg ctcgggcgag
     1561 ctgacgaccg gcttcatcgc cgaggaatac cccgaaggct tcaccggcgc cgccaccagc
     1621 gaggagctga aggccaagct ggcggccatt gccgggttca tcgccacggc gcgggccgac
     1681 cgggcgcgca atgtggacgg tcagctggct ggccacctgc cgccgccgtt cgaatggcag
     1741 gtcaagctgg gcgggagcac cttcgcggtt gaggtcgacg aggacgatgt cgtggtcgac
     1801 ggcaaggtga tcgacctcgc catggaatat acgccgggcg accggatcgt gttcgccgag
     1861 gctgacgacg aggacatcgc cctgaaggtc gagccgacgc gtggcgggct gaagctgacc
     1921 acgcgcgggg cgatccacaa ggtggggatc ctgccggcac aggtggcgca cctcgccggg
     1981 cacatgatcg agaaggtgcc gccggatctt tccaagttcc tgatctgccc gatgccgggc
     2041 ctgctggttt cgctgaacgt ggccgagggc gacaaggtgg aagccggtca gccgctggcg
     2101 gtggtggaag ccatgaagat ggagaacatc ctgcgggcgg agaagtcggc gacggtgaag
     2161 aaggtcaatg ccacggccgg cgagagcctg gcggtggatg cgatcattct cgagctggag
     2221 taggggcggc tcggtccttc cccctcgatg ggggaaggac tggttgcgca cagctctggg
     2281 agctcccacc tcaggccgat gccaacgatc tggaccatcg gttacgagaa gacgacgcag
     2341 gatgcgctga ttgccgcgct gcaggaggcg ggggtggagg ttctggccga tatccgtgcg
     2401 ctgccgctgt cgcggcggcc ggggttttcc aagtcctcgc tggcggcggc ggcgcgggcc
     2461 gggggcatgg aatatcgtca tctgaagccg ctgggcaccc cggccgaggg ccgcgccgcc
     2521 gcgcggcggc atgatcatgc cgcgctcgag cggatctatg ccggccagct ggaattgccc
     2581 gaggcgctcg ccgccatggc cgagctgcgt tcccttgcca gtgaacagaa ggtcgcgctg
     2641 ctgtgctacg agcgcgaggc cgccgaatgc caccgcagct tgctgatcgg cgcattgctg
     2701 gccgatttcg cggtgatcga tctcaagccc tgaaggcggt caggcggtca ggcagtcagg
     2761 cagtctcgcg ggtgaccgag atctcctggc tgttgagcca ggtgtagccg aacgaggtga
     2821 ggaacgagac atcggccgcg tcccgcccga tgccgatctt ggccatgtcg gccccggtcg
     2881 ccatgcccgt gggatcgacc aggtgccatt cgccggccag gaacacttcg cagacggcgt
     2941 ggaaatcggg cgggctcaca tcgggcgcat agacgctggc gatgcgcgcc gggatctggg
     3001 cggcgcgggc catggcgatc aggacatggg catagtcgcg gcagacgcct tcgcggctga
     3061 cgaaggtatc gatggcggtc gtggccgagg tgctgacgcc ggcgacgtag cggaaattgg
     3121 cgcgcaggta gtcgctcatg gcgaggatca gggcgccgcc cgacaggccc ggaaactcgc
     3181 cggcgacgaa gcgctcgaag ctgtgggacg gacagtagcg ggattcgagc aggtaaggca
     3241 cggttggccc aggcagcagg tgcagcggca tggccggaag gtcggcaagg tcgcgggtga
     3301 tccgctgaac attgaccgtg gcgtggtaat cgacggtgag gcgttcggcc acgcgcagca
     3361 tgatccgttc gccgatgctg tcctgcccgg gaacgcgggc gaagtgttcg accgggctca
     3421 ggccgatcca cggctgcgag acgtcctgct cggggatcat tgccgcctcg acctgcagga
     3481 tcacggcggt cggctcggga aagccgtagt cgagccgggt gtggatcgcg agctgcatgt
     3541 ggggaggaac ccgccagtcg tggaaaagat gccttagcgc ctggggtcag gccggtgcgc
     3601 cctgcaattg accatcgagg aacgcggcga catccgccag gtccacgtcc ttggccagat
     3661 aggccccccc gatcccgcgc aggaggagga agggtagcgt gccggcgtcc atcttcttgt
     3721 cgtggagcat gtgggccacg agggcgcggc catcgacgtt gaggccgagc tcgaccagtt
     3781 ccgacatcat gccgagcgag cggaagtgtg ccgcgacgcg ctccgcatcg gcagcgggca
     3841 gttcgccgcg gcgggcggaa tagcgggcgg cgaggaccat gccgagggcc acgctttcgc
     3901 cgtgaaggag gcgggcggaa tagccggttt cggcttccag cgcatggccg aaggtgtggc
     3961 cgaggttgag cagggccctg aggccggtgg tctcgaactc gtcctgctcg acgatgcgcg
     4021 ccttggcctc gacggacctg gcgatggcgt gttcgcgggc ggcatggtcg ccgtccatca
     4081 gggccgcgcc gttggcttcg caccactgga aaaattccgc gtcgcccagc aggccgtact
     4141 tgaggacctc ggcatagccg gcacccagtt cgcggcgggg cagggtctcg agcgcctgcg
     4201 gatcggcgag gacgagggcg ggctggtgga aggcgccgac gaggttcttg cccgcgggtg
     4261 tgttgatcgc ggtctttccg ccgacgctgg aatcgacctg gctgagcagg gtcgtcggca
     4321 gctggatgaa ctggcagccg cgcttgagaa tggcggcggc aaagccggtg agatcgccga
     4381 tcaccccgcc gccgagcgcg ataaccgcgt ccttgcgttc gacgccttcg gccagcagcc
     4441 agtcggtcac ctcggcgaga tgttcccacg acttggtctg ctcgcccgcg ggcaggatct
     4501 tccagacggg ctcgaacccg gccgcggcga gcgaggcgcc gaccgtttgc ccccaggcgt
     4561 tgtgaacatt ggcgtcggtg atcaccggaa cccgcttctt gcgcaggaac ggggcgcatt
     4621 cctcgcccgc gcgggcgagc aggccggcgg cgatgcgcac ctcgtagggg cgtcccgcga
     4681 cgtcgacggg gatcacagcc attgcgctac acctttcatg accgccgcca gcgtctggga
     4741 atggggctgg ttgccgctct gcacgtggac cggcgcctgg gcataggcgg gttcgcgctc
     4801 cgccttgagg cgggtgagga tctcgcgcgg gttgccggtg cgcaggagcg ggcggttgtc
     4861 cttgcggccg acgcgctgga gcagggtttc gacgtccgca tcgagccaga cggcgatcgc
     4921 cttggcgagg atcagggcgc gggtttccgg gttgacgaag gcgccgccgc ccgtggcgat
     4981 gatcatcggg cgcttgccgg cgctctcctc gatcaatcgc tggatcaccc ggcgctcgcc
     5041 gtcgcggaag tagtcctccc cgtaagtggc gaagatctcg ggaatcgtca tctgcgcggc
     5101 ctgctcgatc gcttcgtcgg cgtcgatgaa ggcgaggccc agcaggttgg ccagccgccg
     5161 gccgaccgag ctcttgccca cgcccatcat gccgacgagc acgatcggcc gtttcagctg
     5221 ccggctgatc agccgcgcca gtgccgcatt atcggccgga tcgaggcggg cgtcgtcatg
     5281 ttccattgca gggctgccta tagttgcgct actcgcccgt gcaagcgcgt tgcattggcc
     5341 cggcatgggc cgggcggggc gcgggaacct ggctccggat gggtcgcaca gtggcagcag
     5401 gcggaatttc gattggcatg aaacagcccg gacgcatcgc tctggtggtc atcgtggccc
     5461 ttggactggc cctcctggcc tgggcctggc acaatggcgg gcgcgaaccg ctgcacgaga
     5521 tcgccgttcc cgtggcggtt ccggagagcg cgaagtgaag cgcgccctgc tgctggcggg
     5581 cgccgccctg gcgctgacct ccgccttcgt ggctgcgcag gacgcgcccg aatcgctgtt
     5641 gcctccaggc tttgatcgtc ccgcgccgcg ccccgccagg gctgctccgg cgcctgctcc
     5701 cactggcccc gctgccgcgg caccggcgca gggtcagtct tcgcccgtga tccagccgct
     5761 gccggttgct gctccgcgat cgggtgcggt cgccttaccg tccaacctgc cgtcgctgga
     5821 ccagctcgag gcgatgtcgc cggacgagct cgacaaggtg ctcgacctgc gcccgaagga
     5881 cgacattccg cccgccgccc gccgggccat gacccgcgtc ggcgtcatcg acgagggcga
     5941 gggcgggctg ccgttctgga acctgggcaa gcaggacccc gaccttgtcc gcgccgtgct
     6001 gcagggcaat tcgggcaagc tggtctcgcg ctggggccat atcctgctgc gccgggccgt
     6061 ggcttcgcgg cttgacgcgc cggcgggcat ggaccctgcg gaattcgccg cgctgcgcgc
     6121 ggcgctgctg gtccgcatgg gcgaggggga cggggcgcgc tcgctcgtcc aggacgtgga
     6181 cacggcaaat tactcgcccg agctgaccca ggccgcgctt gatgcctatg tggcgacggg
     6241 agatttcacc gggatatgcc cggtgatcgc gctgcaaggc gcaagccgca aggacaagcc
     6301 gtggcaggtg ctccaggcga tgtgcgacgg ctttggcggc gataccagcc gctcgatggc
     6361 ctcgctcgac cggctgacct actatggcgc catgccgcgg atcgacatgc tgctggcgca
     6421 gaaatacgcg ggcgccgccg gcaaggcgcg caaggcggtg accatcgagt gggacaaggt
     6481 caccgacatg accccgtggc gctatgcgat gaccacggcg gtgggtctcc agccgcccgc
     6541 ggcgctgatg aaggggctgc cgctcgacta tgccttccag gcggcgaccg cgccggccct
     6601 cgcgctcgac ggccgggcgg cggcggcgga caaggcggcg gcctgcggcg tgctctcgag
     6661 cgcggcgatg gtcgatctct atgggcagtt gttcgacgag gacgatgcga acggcgaatg
     6721 ggcgcagcgc gcggccaagc tgcgcgatgc ctatgtagcc gagacggtgg gcgaccgcgc
     6781 cggggcgatc cgcgacctgt ggggtgatga gagcgatccg ctaaggcgct attcgcgcca
     6841 ggtgctgacc gcctatgccg ccgcgcggtt gccggtgagc gcggacctgg cgggcgaggc
     6901 cgggccgctg atcgccgcga tgctttccgc cgggctcgac ggcaatgcca tgcgctgggc
     6961 gggcgtggtg aagagcggga gcgagggctg ggccctgctg gccctggcca atcctgcggc
     7021 caatcccgcc gccgaccagg gcgggatcga cgacttccac ggcaacgaca agagcaaggg
     7081 ctcgcgcaaa tcggccttcc tggtggcggg acttgccggg ctgggccgcc tgccggtggc
     7141 gacagcggcg agcatggccg agaaatacgg cttccagctt gatcggcaat cgcgctgggc
     7201 gcaggcgatc gaccgggcgg cgggttatga caaccgcgcc ttggtggcct tgctggctgg
     7261 gctgggcatg cagggcgaca gctgggagcg gatgaccccg cgcaacctct atcacgtggt
     7321 ggcggcgctt aaccgggtgg gcctttccgc cgaggcgcgg atgatcgcgg ccgaagccgt
     7381 ggcgcgcggg tgaggcaggc gcgcgccgcc gatcctgtcg agttgtttct ggcgatgctg
     7441 gctgccgaac ggggcgccgc tgcgaatact cttgccgcct atcgtcgcga tctggaaggc
     7501 gcggaggaag tgctgggcga cctgacgcgg gccgacggtc cggcgctggc cgggctgggg
     7561 caggcctggg cgggactggc tccggcaagc ctcgcccgca aatgctcggc cctgcgccag
     7621 ttctatggct tcctggtgga tgagggcatg cgtgaggacg atccctcgcc cgccctgccg
     7681 cggccgcgca cccgtcgccc cctgccgcgc ctgctcagcc acgaggaagt ggagcgcatg
     7741 ctgggccggg cggaagagga ggccgccggg ggcacgcccg ccgcgctgcg catgctggcc
     7801 ctgctggagc tgctctatgg ttcgggcctg cgggcgagcg agctggtttc gctgtcgctg
     7861 gccgccgtac cgcgcgacgc gccgtttctc catgtgaccg gcaagggcgg gcagcagcgg
     7921 ctggtgccgg tcagcacccg ggcggcccag gcgctcgcgc ggtggctggc ggtccggccc
     7981 ggcggcggta cgcggctgtt tccctcaagc tcggcgcagg ggcacctgac gcgggtgcgg
     8041 ttgttccagc tgctgcgcga gctggcgacg cgggcaggga tcgcggctga aaaggtctcc
     8101 ccccacgtgc tgcgccatgc ctttgccacc cacctgctgg agggcggggc tgacctgcgc
     8161 gtgctgcaga cgctgctcgg ccacgcggat atcgccacga cccagatcta cacccatgtc
     8221 gaggcggcgc ggctggtcgc gctggtcaat gcgcggcatc cacttgcggg gggcagcgcg
     8281 gcggggacgg gcagtgcgga tggacttgcc cgaggcagcg cggcggacta acgcggcccg
     8341 atgatctcgt ttctcgaatt cgaaaagccg gtcgccgcgc ttgaggcgcg cattgtcgaa
     8401 ctgcgggcga ccgccgccga cggcgacatc gatctcgcct cggaaatcga gcggctggaa
     8461 aagaagagcg cggccctgct cgccagcacc taccaggcgc tgacgccgtg gcagaagacg
     8521 caggttgccc gtcacccggc gcggccgcac ttcgtgaact acatcgcggg cgccttcagc
     8581 ggcttcatgc cgctcggcgg cgatcgcacc tttggcgacg accaggcgat catcggcggc
     8641 ttcgccatgc tggggcaccg ccgggtcatg gtcatcggcc acgagaaggg ccacgacacc
     8701 cagacccgca ttcgccacaa cttcggcatg ggcaagcccg agggctaccg caaggcgatc
     8761 cgcctgatgg aactggccgg gcggttcggc ctgccggttg taacgctggt cgacacctcg
     8821 ggggccttcc ccggcgtgga ggcggaggag cgcggccagg ccgaggccat tgcccgctcg
     8881 accgaggcct gcctgagcct gcccgtgccg atggttgcgg ccatcgtggg cgagggcggt
     8941 tcggggggcg ccgtggcgct cgccagcgcg gagcgggtgc ttatgctcga gcatgcggtc
     9001 tattcggtga tctcgccgga aggcgcgagc tcgatcctgt ggcgcacggc cgaaaaggcc
     9061 cccgacgccg cagaggcaat gaagatgacc gcgcaggacc tgctcaagct cggcgtgatc
     9121 gaccggatcg tgccggagcc cgtcggcggg gcgcaccgcg atccgctgcg cacggcggtt
     9181 tcgctgggtg cggcgatcgg cgaggaactg gacggcttgg ccaaggccaa gccatcggac
     9241 ctgatgaagc gccgcgagga gcggttcctc gccatcggcg ggtgattatt tgatctagtg
     9301 cgccctccac ttcggcggag ggcttgcggg ctggtgccgc aagggaaagc ccggacggcc
     9361 ttccgtattt caacaaacac aaggggcggc aggttgccct gccgcccctc gatttgttgg
     9421 acttgcgtcg gctgattacg agttggcggc agtcagctgc gacgaggtct tggagaaggt
     9481 ggtgttcagc tgcgagccga ggcccgaaac cgtggtcatc acggcgacgg cgataagggc
     9541 ggcgatcaga ccgtattcga tcgcggtggc gccagcttcg tcgcggatca ggttcttgaa
     9601 gaaggtcatg tctagtctcc tggtttagca gtcttggtac ccggctcttc ccgtcccacc
     9661 ggtcggagcg cctttcgaag tagcaggcag tcgttcacaa aaggttaagc gaaaacacgt
     9721 gtccttatga tgcggttgcc tgcgccatcg tcgagccaac gttagaccac atgcggttcg
     9781 tttcgttggc gacgccctgg accgtgaaca tcacggtcac gacaaccagg gcggcgatca
     9841 gcccatattc gatggcagtt gcaccgctgc attcgcggag catttgccga atggtgatca
     9901 tgaggttcat gaaaagacct gtcgaatatt gcgatgaggt cgttttggca gtggcgtcgt
     9961 taccgccgcg ttaggcggca gggttaacgt gcaaaaaagt ccgacagcag ggttttccgc
    10021 aatgccgtcc tgtcgggcag ttctacgggg aattggctaa aaaattatgg atgaaattcc
    10081 gacacctgtg ctcgtcgtcg cggcggcgct gatcgcggcc gacgggcggg tctgcctgca
    10141 gcagcggccg gagggtaaaa atcacgcggg tctctgggag tttccgggcg gcaaggtgga
    10201 accgggcgag tcgcccgagg cagcgctggt gcgcgagatc tgcgaggaac tggggctgac
    10261 gctggaggcg gcagaactga tgccgtgcgg gttcgcgggc gatgccggga ttgtcatcct
    10321 gctatatgcc agccgcgcat ggagcggcga ggcggggccg ctcgaaggcg gcgcactggg
    10381 ctggttcgcg cccgaggcgc tggccggcca gccgatgccg ccactcgatt atccgctcgc
    10441 ccggcaggtc tgcaaacttc ttgccggagg gatgatttag gcattgccaa gccgcaggcc
    10501 ctgtcctatt ggcgccgctc ccgagggcgc ccgtagctca gctggataga gcatcagact
    10561 acgaatctga gggtcggacg ttcgaatcgt tccgggcgcg ccaagggaaa tccaaggaaa
    10621 atcgagcacc tcccccgatc aggggtgagg gccgattggc ccaaaataag cgcataggta
    10681 gcaccccacc caaactcgcg tcagcttgcg ggtcggagca tctgcgatca tcatctcacc
    10741 atgctggaac ccgaatcgaa ggcggctcta cgtaccgagc acctcgaacc cgcgtcgatg
    10801 gatccgagcg ggggtcaagg agaggagatc tcgtgaccga cgatgacggc atcaggatca
    10861 tcgcaagcaa ctcggaagcg gaactcggcg cccggcttgc gctcggagac ctgagcttta
    10921 acttgcgcga gctcgcggcg aacatgctca gggtagtgcg gggtgcgggg cggcctgaga
    10981 atctcggccg gcagatggcc gaagtcgtcg ctctctatgc ccgctaccgc gatcaggctg
    11041 gagcctatcc gtcgaactac gacatccacc aagcgatcag ccttcggtcg gagggcaacg
    11101 cccggaagca caccgacaag tggtctcacg ccatcgacga catcgtccag ggaagcctgc
    11161 agatcgcggc atcgcagctg ctcggccaat cgacacagga ggccgccggc gagagcgagt
    11221 taatggaggg agtcgcccgg atcgaagcga tccgcgaggc gaacaggctt gaggcgttgg
    11281 agcggaggcg gtctgagctg gcgttcagca agagcaagag cggtgccaag aggaagcccg
    11341 taaggccaag gggcgagtga ccccgtgatg atggagaggt ccgacgtcga tttggaccgc
    11401 gtgcaccagc tggtcgcgac cgctaccggg gacgacgtgc agctcagggc tcgtgcccgc
    11461 tggttcgtcg actggtgcgc gagcaagtac gataccgaag gggagcggcc atagggggag
    11521 cgatgtgaaa ccgaacaggc catggcgcct cgggtgcgca acgccgttgg tcctgggtta
    11581 tatcgcgttc gcgatttatg cctggttcga cttcgcaagc accaaccatg acgggctcgc
    11641 gaacctcggc ctgattgtgg tgaccgcgcc cgttgccgtc gtaggctttg tcttgtccgg
    11701 tggcggctca tttgccctga tccccgaagg tcgcggctac ctggcagatc acgccatcta
    11761 ctacgtccct gctgttgcct gtaccgcgct ttttctctgg ctgattggcc ggcggatcga
    11821 acgggccttc aggagcgcgg attccacgca cgacgatggt cccccggcgg ggccttgacg
    11881 ctgcg
//
LOCUS       DATNBV010000096         2052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2919896,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000096 DATNBV010000000
VERSION     DATNBV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2919896"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VN627_02320"
     CDS             complement(<1..1176)
                     /locus_tag="VN627_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HXD02120.1"
                     /translation="MTAADGDDAGLGVFARQIPTILLQRRWIILGIWLAGMVAAAIAL
                     AIIPTEYRSKAVMVITPPQVPEEMVGNAIGDNVERRLDTIRQQIISRPDLIAIINRNG
                     LYARQRESKPLSEVIDEMRRQIVLAPIESPGGSSGNNPQEVAFELSFDYPDPDAARAV
                     AQQLMERIVSIYSARTSAQASNAVDYLGSQQATLQRQISEVEGQINAVSSQNGRALAG
                     MSAPIINAGGNSSEAMILELQRENSQLLQRKSSGSGDPSSAIVAAAEAQLAAARATYA
                     DGHPDVIQARQRLAEARKLARESGPSSVGASIDQQIAFNNSQIASLRAQSASDRAQAA
                     MALSAQTRGPAAQQEITALQQKLTGLNQQYQTISAKYIAAQANARANDEQLGRQLSVI
                     "
     gene            complement(1173..>2052)
                     /locus_tag="VN627_02325"
     CDS             complement(1173..>2052)
                     /locus_tag="VN627_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02121.1"
                     /translation="FGPALPPGMPGGSGGPSDTGTTPPIITVPAPIGAGAGLPPDATV
                     IGRRISQHAFGGGINVELRTSARSTLSLAADANEYRYSDPLLSNYRMFSQAAAYSHRL
                     TEATTVQARMSVAEVDYHSGEHDVILTPMLGVSRKLGSNFDLAVYGGASIVRSRRPDG
                     TRIHSDTIALSAQLCGHYARRDFCISGQRQALPSALGGVRPSTNVQATYTDRLNRMDT
                     LSLNLAFDRRGRDSQNIFNSETMANGTVRYDHRFGDRLAVFASAGYTRLWESSVPSRS
                     EVRGTVGIAFRLGDRG"
BASE COUNT          298 a          650 c          731 g          373 t
ORIGIN      
        1 gatgacgctg agctggcggc caagctgctc gtcattcgcg cgggcattgg cctgagcggc
       61 gatatatttg gccgagatgg tctgatattg ctggttgagc ccggtcagct tctgttgcag
      121 cgcggtgatt tcctgctggg ccgccgggcc gcgggtctgc gcgctgagcg ccattgccgc
      181 ctgggcgcga tcggatgcgc tctgcgcgcg cagcgaggcg atctgggaat tgttgaaggc
      241 gatctgctgg tcgatcgagg caccgaccga gctcggtccg ctttcgcggg ccagcttgcg
      301 cgcctcggcc aggcgctggc gggcctggat gacgtccggg tggccatcgg cataggtcgc
      361 gcgcgcggcg gcgagctgcg cttcggcggc ggcaacgatg gcactgctcg gatcgccgct
      421 accgctcgac ttgcgctgga gcagctgcga gttctcgcgc tgcagctcga ggatcatcgc
      481 ctcgctgctg ttgcccccgg cattgatgat tggagccgac atgccggcaa gcgcccggcc
      541 gttctgcgag ctgacggcat tgatctgccc ctcgacctcg ctgatctggc gctggagcgt
      601 ggcctgctgg ctgcccaggt aatcgaccgc attgctggcc tgggccgagg tccgggccga
      661 gtagatgctg acgatccgct ccatcaattg ctgcgcgacg gcccgcgcgg catccggatc
      721 gggatagtcg aacgacagct cgaacgcgac ttcctgcgga ttgttgccgg agctgccgcc
      781 cggcgattcg atcggcgcca ggacgatctg gcggcgcatc tcgtcgatca cttccgacag
      841 cggcttgctc tcgcgctggc gggcatagag gccattgcgg ttgatgatcg cgatcaggtc
      901 ggggcggctg atgatctgct ggcgaatggt gtcgagccga cgttcgacat tgtcgccgat
      961 cgcgttgccg accatttcct ccggcacctg cggcggggtg atcaccatga ccgccttgga
     1021 gcggtattcg gtcgggatga tcgccagggc aatggccgcc gcgaccatcc cggccagcca
     1081 gatgcccagg atgatccagc ggcgctggag gagtatcgtc gggatctgcc gggcgaatac
     1141 gcccagtcct gcatcgtccc cgtcggctgc tgtcaccctc gatctccaag cctgaatgca
     1201 attccaaccg tgccgcgcac ttccgagcgg ctcggcacgg agctttccca cagccgggtg
     1261 tatccagccg atgcgaacac ggccaaccgg tcgccaaacc gatgatcgta gcggaccgtt
     1321 ccattggcca tggtctcgct gttaaagata ttctggctgt cgcggccgcg gcggtcgaag
     1381 gcgaggttga gcgagagcgt atccatgcgg ttgagccggt cggtataggt ggcttgtaca
     1441 ttggtggagg gccgcacccc tccaagcgcc gagggcagtg cctgccgctg gcccgaaatg
     1501 cagaaatccc gccgcgcata atgtccgcac agttgcgccg agagcgcgat agtgtcggaa
     1561 tggatgcggg tcccatccgg tcggcgcgag cggacgatcg aggcgccgcc gtagaccgcc
     1621 aggtcgaagt tgctgccgag cttgcgcgag acgccgagca tgggcgtgag gatcacatcg
     1681 tgttcgccgc tgtggtagtc gacctcggcc accgacatcc gggcctgcac ggtcgtcgcc
     1741 tcggtcagcc ggtgcgagta ggccgcggcc tggctgaaca tccggtagtt ggacagcagc
     1801 gggtcgctgt agcggtactc gttggcgtcg gcggccagcg agagggtgct gcgggcgctg
     1861 gtgcgcagtt cgacgttgat cccgccgccg aaagcgtgct gggatatccg ccggccgatc
     1921 accgtggcat cgggcggcag acccgcgccc gcgccgatcg gcgcggggac ggtgatgatc
     1981 ggcggggtcg taccggtgtc gctcggcccg ccgctgccgc ccggcatgcc cggcgggagc
     2041 gcgggcccga ac
//
LOCUS       DATNBV010000097         2719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1647931,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000097 DATNBV010000000
VERSION     DATNBV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647931"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VN627_02330"
     CDS             <1..680
                     /locus_tag="VN627_02330"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXD02122.1"
                     /translation="CQITREDQDQAAVESHKRATAAIAGGYFKSQILPVEIKTRKGVT
                     VFDTDEHVRADATVESMAALKPVFKKDGTVTAGNASGINDGAAAVVLASGKAVAEKGL
                     KPMAKILAWGHAGVEPSTMGLGPVKAVPVALKRAGLTLDQIDVIESNEAFAAQACGVA
                     KSLGFDPAKTNPNGSGISLGHPIGATGAILTVKAIYELQRTGGKYGLITMCIGGGQGI
                     AMVVERV"
     gene            683..1120
                     /gene="mce"
                     /locus_tag="VN627_02335"
     CDS             683..1120
                     /gene="mce"
                     /locus_tag="VN627_02335"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HXD02123.1"
                     /translation="MKLGRMNHIGVATPDLDESIAFYRDVMGATDITEPFVLESQQVR
                     VCFVNTPGENGTAGTQVELLQPTTPDSAVGKWLEKNPLGGQHHICYEVPDIHAAKAWF
                     EGQGKRVLGEPRIGAHGTLIFFVHPKDMGGQLTEIMETPKAAH"
     gene            1120..1902
                     /locus_tag="VN627_02340"
     CDS             1120..1902
                     /locus_tag="VN627_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXD02124.1"
                     /translation="MSVVTASLDGAVATIAIARPDKLNALTPRVFAELSTAIDEAVAQ
                     GARAVVLTGEGRFFCSGADLVADEYGYEGLPEDLGELLEEHYAPFSRKLAALDLPVVT
                     ALNGPAVGAGLSIALSGDIVVMGEGGYLLLAFVNIGLVPDAGATWLVARSAGRARALE
                     MAMLGERLGAAEAKAAGLVTRVVPDGEVLATAQALAARLAAGPALAIGAIRKQVAFAL
                     DHDFSATLDHERDNQRRLGRSADFAEAIAAFGEKRKPQFKGR"
     gene            1921..>2719
                     /locus_tag="VN627_02345"
     CDS             1921..>2719
                     /locus_tag="VN627_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXD02125.1"
                     /translation="MTDKPSIDQWQAAAAKEVKGKDLTWQTPEGIAVKPLYTADDVTV
                     EPGLPGFAPFTRGVRASMYAGRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLSVAFD
                     LATHRGYDSDHPRVVGDVGKAGVAIDSVEDMKILFDGIPLDQMSVSMTMNGAVIPILA
                     FFIVAGEEQGVDRKLLDGTIQNDILKEFMVRNTYIYPPEPSLRIVSDIFAFCAAEMPR
                     FNSISVSGYHMQEAGATADLELAYTLADAVEYVRTGVRAGLDVDAFAP"
BASE COUNT          464 a          897 c          946 g          412 t
ORIGIN      
        1 agtgccagat cacccgcgag gaccaggacc aggccgcggt cgaaagccac aagcgtgcaa
       61 cggcggcgat cgccggcggc tatttcaaga gccagatcct cccggtcgag atcaagaccc
      121 gcaagggcgt gaccgtgttc gataccgacg agcatgtccg cgccgatgcc acggtggaat
      181 cgatggcggc gctcaagccg gtgttcaaga aggacggcac cgtgaccgcc ggcaatgcca
      241 gcggcatcaa cgacggtgcg gccgccgtgg tcctcgccag cggcaaggcc gtggcggaga
      301 agggcctgaa gccgatggcc aagatcctcg cctggggcca tgccggggtc gagccgagca
      361 cgatggggct cggcccggtc aaggcggtgc cggtggcgct gaagcgtgcc gggctgacgc
      421 tcgaccagat cgacgtcatc gagagcaacg aggccttcgc ggcccaggcc tgcggcgtgg
      481 ccaagagcct cggcttcgat ccggccaaga ccaaccccaa cggctcgggc atcagcctgg
      541 gccacccgat cggggcgacc ggcgcgatcc tgaccgtgaa ggcgatctac gagttgcagc
      601 gcaccggcgg caagtatggc ctgatcacca tgtgcatcgg cggcggccag ggcatcgcca
      661 tggtcgtgga gcgggtgtga ccatgaaact cggccggatg aaccatatcg gcgttgccac
      721 gcccgatctg gatgagagca tcgccttcta tcgcgacgtg atgggggcga ccgacattac
      781 cgagccgttc gtgctggaga gccagcaggt gcgggtctgt ttcgtgaata cgccgggcga
      841 gaatggcacg gcggggacgc aggttgagct gctccagccg accacgccgg acagcgccgt
      901 cggcaagtgg ctggagaaga acccgctcgg ggggcagcac cacatctgct acgaagtgcc
      961 cgatatccat gccgccaagg cctggttcga aggccagggc aagcgggtgc tgggcgagcc
     1021 gcggatcggg gcgcacggga cgctgatctt cttcgtccac cccaaggata tgggcggcca
     1081 actcaccgag atcatggaaa cgcccaaggc tgcgcattga tgagcgtggt gaccgcctct
     1141 ctcgacggcg cggtggcgac gatcgccatc gcccggccgg acaagctcaa tgcgctgacc
     1201 ccgcgggtct tcgccgagct gtcgaccgcc atcgacgagg cagtcgcgca gggcgcccgg
     1261 gccgtggtgc tgaccggcga gggccggttc ttctgctcgg gcgcggacct tgtcgccgac
     1321 gagtatggct atgaggggct gcccgaagac ctcggcgagc tgctcgaaga gcattacgcg
     1381 ccgttttcgc gcaagctggc cgcgctggac ctgccggtgg tgaccgcgct caacggcccg
     1441 gcggttgggg cggggctgtc gatcgcgctg tcgggcgata tcgtcgtcat gggcgagggc
     1501 ggctatctcc tgctggcctt cgtcaacatc ggcctggtgc ccgatgccgg ggcgacctgg
     1561 ctggtcgcgc gcagcgcggg ccgggcccgg gcgctggaaa tggccatgct gggcgagcgg
     1621 cttggcgcgg ccgaagccaa ggcggccggg ctggtcacgc gggtcgtgcc cgatggcgaa
     1681 gtgctggcca cggcccaggc gcttgccgcc cgccttgccg caggaccggc gctggcgatc
     1741 ggcgcgatcc gcaaacaggt ggccttcgcg ctcgaccacg acttttccgc cacgctcgac
     1801 cacgagcgcg acaatcagcg gcgacttggc cgctccgccg atttcgccga ggcgatcgcc
     1861 gctttcggcg agaagcgcaa accgcaattc aagggccggt aaggccgcag ggatccgagc
     1921 atgaccgaca agccgagcat cgaccagtgg caggccgccg cggccaagga agtgaaaggc
     1981 aaggacctca cctggcagac gccggaaggc attgccgtca agccgctcta caccgccgat
     2041 gacgttaccg ttgaaccggg cctccccggc ttcgcgccgt tcacccgcgg cgtgcgcgcc
     2101 tcgatgtatg ccgggcggcc ttggaccatc cgccagtatg caggcttctc gaccgccgag
     2161 gaatcgaacg ccttctatcg ccgcaacctc gcggccgggc agaaggggct ttcggtggct
     2221 ttcgacctcg cgacgcaccg tggctatgac tcggaccatc cgcgcgtggt cggcgatgtc
     2281 ggcaaggcgg gcgtggcgat cgacagcgtc gaggacatga agatcctgtt cgacggcatt
     2341 ccgctcgacc agatgtcggt ttccatgacg atgaacggcg cggtgattcc gatcctggcc
     2401 ttcttcatcg tcgcgggcga ggagcagggc gtcgaccgca agctgctcga cgggaccatt
     2461 cagaacgaca ttctgaagga gttcatggtc cgcaacacct acatctaccc tcccgaaccc
     2521 tcgctcagga tcgtgtcgga catcttcgcc ttctgcgccg cggagatgcc gcggttcaac
     2581 tccatctcgg tatcgggcta ccacatgcag gaggccggcg cgacggccga cctggagctg
     2641 gcctacaccc tggccgacgc cgtcgaatac gtccgcacgg gcgtgcgcgc cgggctggac
     2701 gtggacgcct tcgcccccc
//
LOCUS       DATNBV010000098         2768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2921552,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000098 DATNBV010000000
VERSION     DATNBV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2921552"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..737)
                     /locus_tag="VN627_02350"
     CDS             complement(243..737)
                     /locus_tag="VN627_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02126.1"
                     /translation="MRKFVISLAAAGAALAVAAPAAAQYYPQPQPYGYGAPYGNAYGY
                     NNYGRIRSLQARVDQLQRRIYQLDRRNILSNGEARRLRGDANDLERRLHRDVRDGRGL
                     SFDEMRQIEYRIARLEQRIYREANDGNRWGNGYNNYNNGYYDRDRDGRDDRYEDDRGR
                     DHDD"
     gene            complement(848..1675)
                     /locus_tag="VN627_02355"
     CDS             complement(848..1675)
                     /locus_tag="VN627_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXD02127.1"
                     /translation="MNDQPQHWLQSAPGDPVLKGVNWGGLKTLYIKEVRRFFKVQMQT
                     VWAPAITTLLYLAIFTVALGGSGRRIMGLHFADFIAPGLIVMAMIQNAFANSSFALLV
                     GKIQGNIVDYLMPPLSTGELIAGLVGASVTRAILVGFAVWLAMLLWPGVSVAVRRPEL
                     VLWFGLMGALLLSFMGMMTSMWADKFDHAAAVTNFVVTPLSLLSGTFYSVHQLSPPFQ
                     AVSHLNPFFYVISGFRAGFLGVSDSPLWVGGLGLLALNVILWAACYRLLKSGWKIKS"
     gene            1733..2395
                     /locus_tag="VN627_02360"
     CDS             1733..2395
                     /locus_tag="VN627_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HXD02128.1"
                     /translation="MSWTEERIERLKKMWHEGATASQIADELGGVSRNAVIGKAHRLG
                     LEQRPSPVKPGEEKEVKKPAAAPPAAASAQAKAAAPAKAEAPEAAPAPAPAPAGHPQR
                     RQLTPQELQYRSIGPGGFIRQGPGEQQAPIPPAPPRRLVPAKPSPEVADKTGLLDLND
                     RICKWPIGHPGEPDFHFCGNTANPGFPYCVEHCGVAYQAQLPRRDRRPPPPLPFGGPR
                     VR"
     gene            2433..>2768
                     /locus_tag="VN627_02365"
     CDS             2433..>2768
                     /locus_tag="VN627_02365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXD02129.1"
                     /translation="MTEPAAIVLVRHGATEWSASGRHTGRSDIPLSPAGETAARALVD
                     RLPPGPYARAWTSPTQRARETARLAGFADAAIVTDLSEWDYGDYEGLTTAEIQAERPG
                     WNLFADGCPN"
BASE COUNT          499 a          970 c          909 g          390 t
ORIGIN      
        1 ccgtagagat aaacgccttc accgcggacc gggcgaaccg gcgagcgcgg atagacgggc
       61 atgagcggcg tgatggccat ggatggcggc tcctcgattg ggcgtttgtg gagcggcccc
      121 cgggccgccg cgcagcgcct atagccagcg ccgcgaaaaa gggcaaagcc gagcggcgga
      181 caagagaaaa ggcgccccgg aatcgctccc gaggcgcctc ttcttaaacc tggccgagcg
      241 acctagtcgt cgtgatcccg gccgcggtcg tcctcgtaac ggtcgtcgcg gccgtcgcgg
      301 tcgcggtcgt aatagccgtt gttgtaatta ttgtagccgt tgccccagcg gttgccgtca
      361 ttggcttcgc gatagatgcg ctgctcgagc cgcgcgatgc gatactcgat ctggcgcatc
      421 tcgtcgaagc tcaggccgcg gccatcgcga acgtcgcgat gaagccgccg ctcgagatca
      481 ttggcgtcgc cacgcaggcg gcgggcctcg ccgttgctca ggatgttgcg gcggtcgagc
      541 tggtagatcc gccgctgcag ctgatcgacc cgagcctgga gcgaacgaat ccggccgtaa
      601 ttgttgtagc cgtaggcgtt gccgtagggc gcgccgtaac cataaggctg gggctgcgga
      661 taatattggg ccgcggcggg cgccgcgacg gcaagggccg ctccggcggc agcaagggaa
      721 atcacaaact tacgcatcac ggctctcctt tgagctcgag ggtggttgcc gctgttgtgc
      781 gccgattcgc ttgatgcggg gctgaacgcc caagttatcc gccgttcaac aacattaacg
      841 cgctcactca gctcttgatc ttccagccgc tcttgagcag ccggtagcag gccgcccaca
      901 ggatgacgtt gagcgccagc aggccgagcc cgccgaccca caagggcgaa tcgctgacgc
      961 cgaggaagcc cgcgcggaag cccgagatca cgtagaagaa cgggttcaag tggctgaccg
     1021 cctggaacgg cggcgacagc tggtggaccg agtagaaggt gccggagagc agcgacagcg
     1081 gggtcaccac gaaattggtc accgccgcgg catggtcgaa cttgtcggcc cacatcgagg
     1141 tcatcatccc catgaacgac aggagcaacg cgcccatcag cccgaaccac agcaccagct
     1201 cgggccgtcg cactgcgacc gacacgccgg gccacagcag catcgccagc cacaccgcga
     1261 agccgaccag gatcgcccgc gtcaccgacg ctccgaccag cccggcgatc agctccccgg
     1321 tcgacagcgg cggcatcaga tagtcgacga tattgccctg gatcttgccg accagcagcg
     1381 cgaagctcga gttggcgaag gcgttctgga tcatcgccat gacgatcagg cccggcgcga
     1441 tgaagtcggc gaagtgcagg cccatgatcc gccgcccgct gccgcccagg gcgacggtga
     1501 agatggcgag atacagcaag gtggtgatcg ccggcgccca cacggtctgc atctggacct
     1561 tgaagaaccg ccgcacctcc ttgatataga gggtcttgag gcctccccaa ttgacgccct
     1621 tcagcaccgg gtccccgggc gcgctctgaa gccagtgctg cggttggtcg ttcacggatg
     1681 aggcgcctat cggctgggga ccggccgggc aagtttggag cagagacaaa gaatgtcgtg
     1741 gaccgaggag cgcatcgagc gcctgaagaa gatgtggcac gagggcgcca ccgccagcca
     1801 gatcgccgac gagctcggcg gagtcagccg caacgcggtg atcggcaagg cccaccgcct
     1861 cgggctcgag cagcgcccgt cgccggtcaa gccgggcgag gagaaagagg tcaagaagcc
     1921 ggccgctgcg ccgcccgccg cggcaagcgc tcaagccaag gccgctgctc cggcgaaggc
     1981 cgaagcgccc gaagccgcgc ccgcgccggc gccggcgccc gccggccatc cccagcggcg
     2041 ccagctgacc ccgcaggagc tgcagtatcg ctcgatcggc ccgggcggct tcatccgcca
     2101 gggcccgggc gagcagcagg caccgatccc gccggccccg ccgcgccgcc tggtcccggc
     2161 caagccgagc ccggaggtcg ccgacaagac cggcctgctc gatctcaatg accgcatctg
     2221 caaatggccg atcggccatc ccggcgagcc cgatttccac ttctgcggca acaccgccaa
     2281 cccgggcttc ccctattgcg tcgagcattg cggcgtcgct tatcaggcgc agctcccgcg
     2341 tcgcgaccgc cgcccgcccc cgccgctccc cttcggcggc ccgcgggttc gctagcgcct
     2401 ggcggcgacg cgccggatag caatcgccgc caatgaccga acctgcggcc atcgtcctcg
     2461 tccgccacgg cgcgaccgaa tggagtgctt cgggcaggca caccggccgc agcgatattc
     2521 cgcttagtcc cgcaggcgaa accgccgcgc gcgccctcgt cgatcgcttg ccccccggac
     2581 cctatgcccg tgcctggacc agcccgaccc agcgcgcccg cgaaaccgcc cggctcgccg
     2641 gctttgcaga cgctgcaatc gttaccgacc tcagcgaatg ggactatggc gactacgaag
     2701 ggctgacgac ggccgaaatc caggccgagc gccccggctg gaacctgttc gccgacggct
     2761 gcccgaat
//
LOCUS       DATNBV010000099         8500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2921572,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000099 DATNBV010000000
VERSION     DATNBV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2921572"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..829
                     /locus_tag="VN627_02370"
     CDS             218..829
                     /locus_tag="VN627_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXD02130.1"
                     /translation="MNRRAMTKPLARLLPALLAMIVPLALTACEQREPPPLGGAAIGG
                     PFTLVDKAGKTVRWADFAGKWRIVYFGYTWCPDACPTDVGVMMRGFDRFAKDHPDLAG
                     EVQPLFITVDPARDTPARVGEFAAAFSPRLLGLTGTPAQIDQAAKAFAAYYARGKSQP
                     GGGYLMDHSRVAYLMDRDGKPVAMLPVDRDDKAVAAELAKWVR"
     gene            834..1313
                     /locus_tag="VN627_02375"
     CDS             834..1313
                     /locus_tag="VN627_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HXD02131.1"
                     /translation="MTGPDKPFWERPLASLDKAEWEALCDGCGKCCLHKVEDADTGRI
                     WPTNVACKLLDLKTARCADYRNRRKYVSDCVRLTPRLAAELEWLPPTCAYRLRANDEP
                     LPAWHYLISGDRETVRQAGFSVAGQAISEVLAGPLEQHIIWGDETPMEVERVEDGEE"
     gene            1310..2053
                     /locus_tag="VN627_02380"
     CDS             1310..2053
                     /locus_tag="VN627_02380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HXD02132.1"
                     /translation="MSLLPDFLPEFLRGRSAPPTLTLGERVLPIEIRRLSTARRLTLR
                     LAPDGSAIRVTIPHWAPTKEALAFVHSRTDWLETQLGNVPEAAPLAPGAMLPWRGTPH
                     VLHHRTDLSRGVRLVDGALHVGGPAESLVPRLRRWVQAEARALFLADLADYALRAGVP
                     VPALALTNARRRWGSCAAEGTIRMNWRLAMAPDEVRRSVVAHEVTHLVHFDHSPRFHA
                     MLGDLFEGDLNGANRWLKRHGRGLYGPLG"
     gene            2122..2694
                     /locus_tag="VN627_02385"
     CDS             2122..2694
                     /locus_tag="VN627_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02133.1"
                     /translation="MLKQVRHGGSGEVVVGQSRLSLSPPSPYLPAMLSLGKSSYWRNV
                     HPTGAIGDFITVWRQAGKRRWPFVAAALTTTLLVFNIIVQENWKGPPAKPKVTYINSW
                     TADRSDAEIEREIIANQKLQDRLAAEQAVREAKVKEIYRTLGQVSGMDTAAIEQQAAA
                     DAAREKAAHDKAIGLKPAPGSPAAEKPAGH"
     gene            2690..3664
                     /gene="ribD"
                     /locus_tag="VN627_02390"
     CDS             2690..3664
                     /gene="ribD"
                     /locus_tag="VN627_02390"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HXD02134.1"
                     /translation="MDPQDLRWLDAAARLAGRGLPLSRPNPAVGALLVKDGKVIGRGW
                     TQPGGRPHAEAVALAQAGETARGATLYVTLEPCAHASARGPACADLTAAAGLARVVVG
                     CEDPDPRTSGEGIARLRAAGTPVDLVRSPACAASLAGYLVRARLGRPHVTLKLALSLD
                     GCIALADGQSQWITGEAARAHTHAMRARADAILVGGGTLRADRPRLDVRLPGLEARSP
                     ARWVLTRGASPAGWTALLGPEAIHDHPEAQYLFVEGGAGTAAAFLAADLVDRLLLYRA
                     PILIGGGRPGIADLGLTGLAQAHGRWAMADRRTLGSDTCEVYERVRTS"
     gene            3679..4296
                     /locus_tag="VN627_02395"
     CDS             3679..4296
                     /locus_tag="VN627_02395"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HXD02135.1"
                     /translation="MFTGIVTAIGTIREATQKGDLHAVIACPWDPAGIAIGASIACSG
                     TCLTVVERGGSAGDAWFAVDISGETLARTVPGRWVAGGRLNLEPALRLGDELGGHIVT
                     GHVDGVGEIVGICPEGDSRRVGIAAPSALAPYLAPKGSITVDGVSLTVNDVADQADGT
                     CHFALNLIPHTAEVTTLGDLAPGAKVNLEIDVLARYLQRMQNLRG"
     gene            4331..5095
                     /locus_tag="VN627_02400"
     CDS             4331..5095
                     /locus_tag="VN627_02400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXD02136.1"
                     /translation="MKIAVITPYYQEPLAKLRRCHDSVARQVPEADHIMVADGQAQAA
                     VDGWNAVHLKLPVATNDCGDTPRMIGLSLAAARGYDAFLFLDADNWLEPGHVAQLVAA
                     QQESGASVITCPRILRRLDGSALGLCPESDGIAFNDFNCYLFTRAALPLLAALAFGDR
                     ATAYVTDRRLWQHLRSSGCAIARAPEATLNYETRRAMHYQLAGETPPPYALVSYLDQA
                     TGKMIDMTIAEWRAFCRANPHLRIAPVETALTGKPG"
     gene            5200..5598
                     /locus_tag="VN627_02405"
     CDS             5200..5598
                     /locus_tag="VN627_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 family protein"
                     /protein_id="HXD02137.1"
                     /translation="MSILGNIFNKIFHHPAAQQQAQAAQPQQQAQQQQSAAPAAPTGQ
                     PQQQVDVGAVLTQMAAQKGGGGNWQSSIVDLLKLLDLDSSLAARKELANELNVHVGAD
                     GSAEENIALHKAVVQQLAANGGKIPDAMKH"
     gene            5612..5896
                     /locus_tag="VN627_02410"
     CDS             5612..5896
                     /locus_tag="VN627_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02138.1"
                     /translation="MSLFDQLAGQAGGLNLGGIAGRFGLSEEQVRSAAALLLPRSPTR
                     PSTTPRQATTWRSRRALPWHPGAAGPALISQAQANGAEGGVLGSLLRGLG"
     gene            complement(5969..7165)
                     /locus_tag="VN627_02415"
     CDS             complement(5969..7165)
                     /locus_tag="VN627_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HXD02139.1"
                     /translation="MSSTSVLTNLQQQPADALLALIKLHNADPRADKIDLGVGVYRTD
                     TGATPVFAAIKAAERKLLETQDSKAYLGPEGDMGFVHALMPYVFGAANPTMDGRIEGM
                     QTPGGTGAVRLALALAKRAGTARIWMGTPSWPNHAQIVADLGLELKGFVHATKAGLAD
                     MDSLRFAIGQAEPGDAILLHGCCHNPSGIDYSPAQWDEIAGLLRDKGLLPIIDLAYQG
                     LGAGMEEDAYGLRKVLAEVPEALIAYSCDKNFGLYRDRVGAVYIMMADAAQVNAALSN
                     GHALARAAWSMPPDHGAAAVRIILEDPALVQLWQGELDEMRARMRQVRARLAAAGAAG
                     AVDMTPFGTQNGLFSIVPLSPEQVLAMREKHGVYMAGSGRINVAGLTTGNIDKFIAAV
                     ADVTAG"
     gene            complement(7352..8113)
                     /locus_tag="VN627_02420"
     CDS             complement(7352..8113)
                     /locus_tag="VN627_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HXD02140.1"
                     /translation="MLPNLAGNITRRIAAGLAAGLLALAAQPAIAQVRSVDPDAAIDG
                     DLVKTAPAPTISAAPVATASTAAPVVTSPNAEKAAAQASTNPVQAGAQGATYHEDDLI
                     GAAEGVFGKGAHGLADLIKGLLAKQGEPNGYIVGREGGGAIVVGVRYGSGTLYHKIEG
                     QKPVYWTGPSVGLDLGASAGNTFVLVYNLYNTDDLYHRYGAGEGQAYLVGGFHVSYLR
                     RNDVVLIPVRMGVGLRLGLNAGYMNITRKSRWLPF"
     gene            8313..8405
                     /locus_tag="VN627_02425"
     tRNA            8313..8405
                     /locus_tag="VN627_02425"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8347..8349,aa:Ser,seq:gct)
BASE COUNT         1315 a         3030 c         2876 g         1279 t
ORIGIN      
        1 cgtccccatc ctttttcttc accgcgtcga ggaacttgta gcccgcgccc atgaactgtg
       61 ccgaggccgg cgccgccgga agggccagcg ccgccgccac ggccatgccc gcacaggcgc
      121 ccgcgatccg caatccccgc agcttaccaa tcctcacgcg ttcactccct gtcgcatcgc
      181 ccgatcgaca cccttgcccc gcgcggttta gcagagcatg aaccgccgcg ccatgaccaa
      241 gccacttgcc cgcctgctcc ctgcccttct ggccatgatc gtgcccctgg ccttgacggc
      301 ctgcgagcag cgcgagcccc cgccgctggg cggggcggcg atcgggggac cgttcaccct
      361 ggtcgacaag gccggcaaga ccgtgcgctg ggccgatttc gcgggcaagt ggcggatcgt
      421 ctacttcggc tacacctggt gccccgatgc ctgtccgacc gacgtgggcg tgatgatgcg
      481 cgggttcgac cgcttcgcca aggatcaccc ggaccttgcc ggcgaggtcc agccgctgtt
      541 catcaccgtc gacccggccc gcgacacgcc cgcgcgggtc ggtgagttcg ccgcggcctt
      601 ttcgccccgc ttgctgggcc tgaccggcac gcccgcccag atcgaccagg cggccaaggc
      661 ctttgccgcc tattatgcgc gcggcaagag ccagccgggc ggcggctacc tgatggacca
      721 tagccgggta gcctacctga tggaccgtga tggcaagccg gttgccatgc ttcccgtcga
      781 cagggacgac aaggccgttg ccgccgaact cgccaagtgg gttcgctagg ctggtgacgg
      841 gccccgacaa gcctttctgg gagcgcccgc tggcaagcct cgacaaggcc gagtgggagg
      901 cgctgtgcga cggttgcgga aagtgctgcc tgcacaaggt ggaggatgcg gacaccgggc
      961 ggatctggcc gaccaatgtc gcctgcaagc tgcttgacct caagaccgcg cgctgcgccg
     1021 actatcgcaa ccggcgcaag tacgtatcgg actgcgtgcg gctgacgccc cggctcgccg
     1081 ccgagctcga atggctgccg ccgacctgtg cctatcgcct gcgcgccaat gacgagccgc
     1141 tgcctgcctg gcactacctg atctcgggcg accgcgagac cgtccgccag gccggcttct
     1201 cggtggcggg gcaggcgatc agtgaagtcc ttgccggccc gctcgagcag cacatcatct
     1261 ggggcgacga gaccccgatg gaagtggagc gggtggagga cggggaggaa tgagcctcct
     1321 gccggatttc ctgcccgaat tcctccgggg ccgctccgct ccgccgacgc tcaccctggg
     1381 cgagcgagtc ctgccgatcg agatccgccg gctgtcgacc gcccggcgcc tgaccctgcg
     1441 cctcgcccct gacggcagcg cgatccgcgt gacgatcccc cactgggcgc cgaccaagga
     1501 ggccctcgcc ttcgtccatt cgcggaccga ctggctggag acccagctcg gcaacgtgcc
     1561 cgaagccgcg ccgctggcac ccggcgccat gctgccgtgg cgcggcacgc cccatgtgct
     1621 gcatcaccgc accgaccttt cgcgcggcgt gcggctggtc gacggcgcgc tgcacgttgg
     1681 cgggccggcc gaatcgctgg tcccccgcct gcgccgctgg gtccaggccg aggcgcgggc
     1741 cctgttcctg gcggacctgg ccgactatgc cctgcgcgcc ggggtgccgg tccccgcgct
     1801 cgccctcacc aatgctcgcc gccgctgggg ctcctgcgcg gcggagggga cgatccggat
     1861 gaactggcgg ctggccatgg cgcccgacga ggtccgccgc tcggtcgtcg cccacgaggt
     1921 cacccacctc gtccatttcg accattcgcc ccgctttcac gccatgctcg gcgacctgtt
     1981 cgagggcgac ctcaatgggg ccaatcgctg gctcaagcgg cacgggcgcg ggctgtacgg
     2041 gccgctgggg tagccgggcc cgggcacggc gggatgaaaa gcgctgttcg ggaaggcagt
     2101 gccgcgcgtt cggcgccatg gatgctcaaa caagttcggc atggcggatc gggcgaggtc
     2161 gttgtcggac agtcgcgcct ttccctttcg ccccccagcc cctatcttcc cgccatgctt
     2221 tccctcggca agtcctcata ctggcgcaac gtccacccca ccggggccat cggcgatttc
     2281 atcaccgtct ggcggcaggc gggcaagcgg cgctggccgt tcgttgccgc cgcgctcacc
     2341 acgacgctgc tggtgttcaa catcatcgtg caggagaact ggaagggccc gccggccaag
     2401 cccaaggtca cctacatcaa ttcgtggacg gccgaccgct ccgacgcgga gatcgagcgc
     2461 gagatcatcg ccaaccagaa gctgcaggac cgccttgccg ccgagcaggc cgtgcgcgag
     2521 gccaaggtca aggaaatcta tcgcacgctg ggccaggtct caggcatgga caccgcggcg
     2581 atcgaacagc aggcggcagc cgatgccgcc cgggaaaagg cggcgcacga caaggcgatc
     2641 ggcctgaagc ccgccccggg aagccccgct gcggagaaac ccgctggcca ttgatccgca
     2701 ggacctgcgc tggctcgatg ccgccgcgcg ccttgccggg cggggcctgc cgctgagccg
     2761 gccgaaccct gccgtgggcg cgctgctggt gaaggacggc aaggtgatcg ggcgcggctg
     2821 gacccagccc ggggggcgcc cccatgccga ggccgtggcc ctcgcccagg cgggcgagac
     2881 tgcgcgggga gctacgcttt acgtcaccct tgaaccctgc gcccatgcca gtgcgcgcgg
     2941 cccggcctgc gccgatctca ccgccgcggc gggtctcgca cgggttgtcg tgggctgcga
     3001 agaccccgat ccgcgcactt ccggcgaagg catcgcccgc ctgcgtgccg ccggaacgcc
     3061 ggtggacctc gtccgctcgc cggcgtgcgc ggccagcctg gccggctatc tcgtccgcgc
     3121 gcggcttggg cggccccacg tcacgctcaa gctggcgctc tcgctcgatg gctgcatcgc
     3181 gctggccgac gggcaatcgc agtggatcac cggcgaggcg gcccgtgccc acacccatgc
     3241 catgcgcgcc cgggccgatg cgatcctcgt cggcggggga accctgcggg ccgaccggcc
     3301 ccggctcgac gtgcgcctgc ccggccttga ggcacgcagc cccgcgcgct gggtgctgac
     3361 gcgcggcgcg tcgccagcgg ggtggaccgc ccttctcggc cccgaggcga tccacgacca
     3421 tcccgaagcg cagtaccttt ttgtcgaggg cggcgccggg accgccgccg ccttccttgc
     3481 cgcagacctg gtcgaccgcc tgctgctcta ccgcgccccg atcctgatcg gcggcgggcg
     3541 gcccggcatc gccgaccttg gcctgaccgg ccttgcccag gcccatggcc gctgggccat
     3601 ggcggaccgg cgcacgcttg gcagcgacac ctgcgaagtc tacgagcggg tcaggaccag
     3661 ctgagagacc gcatttccat gttcaccggc atcgtcaccg ctatcggcac catccgcgaa
     3721 gccacgcaga agggcgacct ccatgccgtg atcgcctgcc cgtgggaccc ggcggggatc
     3781 gccattggcg cctcgatcgc ctgctcgggc acctgcctga ccgtggtcga acggggcggt
     3841 agcgccggcg atgcctggtt cgcggtcgac atctcgggcg aaacgctggc gcgcaccgtg
     3901 cccgggcgct gggtggccgg gggacggctc aacctcgaac cggcgttgcg gctgggggac
     3961 gagttgggcg gccacatcgt taccggccat gtcgacggag tgggcgagat cgtcggcatc
     4021 tgccccgagg gcgacagccg ccgcgtcggc atcgccgcgc cctccgcgct ggcaccctat
     4081 ctggcgccca agggctcgat cacggtcgat ggcgtctcgc tcacggtcaa cgatgtcgcc
     4141 gaccaggccg acgggacctg ccacttcgcg ctcaacctga tcccgcatac cgccgaggtc
     4201 accacgctgg gcgacctcgc gcccggagcc aaggtcaacc tcgagatcga cgtgctggcc
     4261 cgctatcttc agcggatgca gaacctgcgc ggttgagacg gccgttcggg ccggtgcggg
     4321 aggcgcggcc gtgaagattg cggtaatcac gccctattac caggagcccc tggccaagct
     4381 gcgacgctgc catgacagcg tcgccaggca ggtgcccgaa gccgatcaca tcatggtcgc
     4441 cgacgggcag gcgcaggcgg ctgtggacgg gtggaacgcc gtccacctca agctgccggt
     4501 ggcgacgaac gactgcggcg acacgccgcg catgatcggc ttgtcgctgg cggcggcgcg
     4561 cggctacgac gccttcctgt tcctcgatgc ggacaactgg ctggaacccg gccatgtcgc
     4621 gcagctagtc gccgcccagc aggagagcgg tgccagcgtg atcacctgcc cgcgcatcct
     4681 gcgccggctc gacgggagcg cgctcggcct gtgccccgaa agcgacggga tcgcgttcaa
     4741 cgacttcaac tgctacctgt tcacccgcgc ggcgctgccc ttgctggcgg cgctggcgtt
     4801 cggggaccga gcgaccgcct atgtcaccga ccggcgcctg tggcagcacc tgcgctcaag
     4861 cggttgcgcc atcgcccggg cgccggaggc gaccctcaac tacgagacgc ggcgggcgat
     4921 gcattaccag ctggccgggg agaccccgcc cccttacgcg ctggtcagct acctcgatca
     4981 ggcgaccggc aagatgatcg acatgacgat cgcggaatgg cgcgcgttct gccgcgccaa
     5041 cccgcacttg cggattgcgc cggtggaaac cgcgcttacc ggaaagccgg gctgaccggc
     5101 gcgccggtcg gcccatggcg accacggccc ggcgattggc gcgggccacc ccgccctgcg
     5161 gtgcgttgcc cccgcgcaac ctcaatacag gagcggcgcg tgagcatcct cggcaacatc
     5221 ttcaacaaga tcttccatca tccggcggca cagcagcagg cccaggccgc ccagccccag
     5281 cagcaggcgc agcagcagca gagcgcagca cccgccgcgc cgaccgggca gccgcagcag
     5341 caggtcgatg tcggcgcggt actcacccag atggcggccc agaagggcgg cggcggcaat
     5401 tggcaaagct cgatcgtcga cctgctcaag ctgctcgacc ttgattcgag cctcgctgcc
     5461 cgcaaggagc tggcgaacga attgaacgtc cacgtcggcg ccgacgggtc ggccgaggag
     5521 aatatcgccc tccacaaggc ggtggtgcag cagctggcgg cgaacggtgg caagattccc
     5581 gatgcgatga agcattgagg agaggcgccc catgagcctt ttcgatcagc tggccggaca
     5641 ggccggcggc ctcaacctcg gcggcattgc cggccggttc ggcctcagcg aagaacaggt
     5701 ccgctccgcc gccgccctgc tcctgccaag atcgccgacc cggccgtcga caacaccgag
     5761 gcaagccacg acgtggcgca gcagacgggc attgccatgg caccctggcg cagctggtcc
     5821 cgccctgatc agccaggcgc aggcgaatgg cgccgagggc ggcgtcctcg gcagcctgct
     5881 caggggactg ggctgaaacg actgggctga aacgacaaag gggcgcgagg ccgaaacttc
     5941 gcgccccttt gatgctcagc gcgcccggtc agccggcggt aacgtcggcc acggcggcga
     6001 tgaacttgtc gatattgccc gtggtcagcc cggcaacgtt gatccgccct gacccggcca
     6061 tgtagacgcc gtgcttctcg cgcatggcga ggacctgctc cgggctcagc gggacgatcg
     6121 agaacaggcc gttctgcgtc ccgaacgggg tcatgtcgac cgccccggcg gcacccgccg
     6181 cggccagcct ggcgcggacc tggcgcatgc gggcgcgcat ctcgtcaagc tcgccctgcc
     6241 acagctgtac cagcgcggga tcttccagga tgatccgcac cgcggcggcg ccgtgatccg
     6301 gcggcatcga ccaggcggca cgggccagcg catggccgtt ggacagggcg gcgttcacct
     6361 gggctgcgtc ggccatcatg atgtaaaccg cgccgacccg gtcgcgatag agaccgaagt
     6421 tcttgtcgca cgaataggcg ataagcgcct cgggaacctc ggccaggacc ttgcgcaggc
     6481 cataggcatc ttcctccatc cccgcgccca ggccctgata ggccaggtcg atgatcggca
     6541 gcaggccctt gtcgcgcagc aggccggcga tctcgtccca ctgggcgggg ctgtagtcga
     6601 tcccgctggg attgtggcag cagccgtgaa gcaggatggc atcgcccggt tcggcctggc
     6661 cgatggcgaa gcgcagcgaa tccatgtcgg cgaggcccgc cttggtggca tggacgaagc
     6721 ccttcagctc gagcccgaga tcggccacga tctgcgcgtg gttgggccac gagggggtgc
     6781 ccatccagat gcgcgcggtg ccggcgcgct tggccagcgc gagggccagg cggacggcgc
     6841 cggtgccgcc cggggtctgc atgccctcga tccggccgtc catcgtcgga ttggccgcgc
     6901 cgaacacgta gggcatcagc gcatggacga agcccatatc gccttcgggg ccgaggtagg
     6961 ccttggaatc ctgggtttcg aggagcttgc gctccgccgc cttgatcgcc gcgaagaccg
     7021 gggttgcgcc ggtgtcggta cggtagacgc caacgccaag gtcgatcttg tcggcccgcg
     7081 ggtcggcatt gtggagcttg atcagcgcga gcagcgcgtc ggccggctgc tgctgaaggt
     7141 tcgtaaggac agaagtgctg gacatgggcg cgcctttagc ggccttcgcc catgcggcaa
     7201 agcccttttg ggcagacagg acaggagcgg ccaaccctcc tgactacacc cgcggttgcg
     7261 gagccgtaag gcgaagcaga gcctctactt caacaaaaaa ccggagccgc aaggcgaagc
     7321 ggagcccctt tcttccggcg cttcgcgccg atcagaacgg caaccagcgg ctcttgcggg
     7381 tgatattcat gtaaccggca ttgaggccga gccgcaggcc cacgcccatc cgcaccggga
     7441 tcagcacgac atcgttgcgc ctgaggtagc tcacgtggaa cccgccgacg agataggcct
     7501 gcccctcacc tgcgccgtag cgatgataga gatcgtcggt attgtacagg ttgtagacca
     7561 gcacgaaggt gttgccggcg ctggcgccca gatcgaggcc gaccgagggt ccggtccagt
     7621 agaccggctt ctggccctcg atcttgtggt agagcgtgcc cgagccatag cgcaccccga
     7681 cgacgatcgc cccgccgcct tcgcggccaa cgatatagcc attgggttcg ccctgcttgg
     7741 cgagcaggcc cttgatcagg tcggcgaggc catgcgcgcc cttgccgaac acgccttcgg
     7801 cagcgccgat caggtcgtcc tcgtgatagg tcgcgccctg cgcgccggcc tgcaccggat
     7861 tggtcgaggc ctgggcagcc gccttctccg cgtttggcga ggtcaccacc ggcgcggcgg
     7921 tgctggcagt ggcgaccggc gcggcgctga tggtcggcgc cggagcggtc ttgacgaggt
     7981 cgccatcgat cgccgcatcg ggatcgaccg acctgacctg ggcgatcgcc ggctgggcag
     8041 caagggcgag gagaccagcg gcaagccccg cggcaatacg gcgcgtgatg ttcccggcaa
     8101 ggttcggcag catgatccca tccctccaac ggcgcggtgg cctcttgaga atccatgcct
     8161 ggcgccgccg caatgaaccg gcgatgagcc gagtccgttt tgcgaccgga agagccgtgc
     8221 ggcgtccttt cgccggcacc gcaaaaatgc cgcgcagacc cttgccgccc cgcccacccc
     8281 tcgctatagg ccccgcttcg ccgctgcgat ccggagacgt gggtgagtgg ctgaaaccaa
     8341 cggtttgcta aaccgtcgta ctggtaaatc cggtaccgag ggttcgaatc cctccgtctc
     8401 cgccaaaaac ctactaaaaa caagggttta gctcctccct tcggaggggc ttccctgcgt
     8461 tggttcatgc tggacgtttt gccggtgtaa ggaccttgta
//
LOCUS       DATNBV010000100         2086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_698273,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000100 DATNBV010000000
VERSION     DATNBV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698273"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..822
                     /locus_tag="VN627_02430"
     CDS             94..822
                     /locus_tag="VN627_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HXD02141.1"
                     /translation="MDVLLTLARDQKVDLKKISILELADQYLAFVARARRLRLEIAAD
                     YLVMAAWLAYLKSRLLLPEPPADGEPSGAELAAALAFQLQRLEAMQKAGRDLMAMPQY
                     GRDFFGRGLPEPVQIIEQPVYKLALYDLLKAYGNYPGRRNEGLLQIAPMTLFSMDDAL
                     HRIGEMLGQVLDWTTLQTFLPPGLQRGLPRRAALAATFAASLELARTGRIELRQEGLF
                     GPIFMRRGNGNDNAPDTANTEGLQ"
     gene            819..1613
                     /gene="scpB"
                     /locus_tag="VN627_02435"
     CDS             819..1613
                     /gene="scpB"
                     /locus_tag="VN627_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HXD02142.1"
                     /translation="MTDDRYQLLRLLEAILFASTEPLGEKELAGRMPEGADINGLLQE
                     LKDQYANRGVHLLAFEDRWAFRTAPDLAPKFHIEREVVRKMSRAAIETLAIIAYHQPV
                     TRAEIEEIRGVVISKGTLDMLLEIGWIKPKGRRETPGRPVTWATTDAFLEHFGLESCD
                     ALPGVEELKAAGLLDARATVATLGTQGLPNGAPVAEEEDEEGNISDMLAQQGEDDPEA
                     QAADDAYDAEVLRRVEADEEGAEPDEEDSDDDLSGSAEPKAAAPSE"
     gene            1749..>2086
                     /locus_tag="VN627_02440"
     CDS             1749..>2086
                     /locus_tag="VN627_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02143.1"
                     /translation="MSLSQPQAATPVRFGARHWGWPSRWTILACGLALLIAAPVISVL
                     INAFGARSEIWQHLVETVLGDYVANSFLIMSGVTIGVLIVGVPAAWMTTLCRFPGRRL
                     FDWALLLPMA"
BASE COUNT          342 a          685 c          720 g          339 t
ORIGIN      
        1 ttcgacaggg atttcgcggc cgacgagggg gcgcccgcgg cgccgggcag cgagttcgaa
       61 ctgcatctcg acctcgacgg gttcgaggga ccgatcgacg tactgctgac gctggcgcgc
      121 gaccagaagg tcgatctcaa gaaaatctcc atcctggaac tggccgacca gtatctcgcc
      181 ttcgttgcac gcgcccgccg cttgcggctg gagatcgccg ccgactatct ggtgatggca
      241 gcgtggctgg cctatctcaa atcgcgcctg ctgctgcccg aaccgccggc ggatggcgag
      301 ccgagcggcg ccgagctggc ggccgccttg gccttccagt tgcagcgcct ggaggcgatg
      361 cagaaagccg ggcgcgatct gatggcgatg ccgcaatatg gccgcgactt cttcggccgc
      421 ggcctgccgg agccggtgca gatcatcgaa cagccggttt acaagctggc gctctacgat
      481 ctgctgaagg cctatggcaa ttatcccggc cggcgcaacg aaggcctgct gcagatcgcg
      541 cccatgaccc tgttctcgat ggacgatgcg ctgcatcgga tcggcgagat gctgggccag
      601 gtgctggact ggacgacact gcagacgttt cttccgccgg gcctgcaacg cggcctgccg
      661 cggcgcgcgg ctttggccgc gaccttcgcc gccagcctgg agttggcgcg caccgggcgc
      721 atcgagctgc ggcaggaggg attgttcggc ccgatcttca tgcgccgcgg caacggcaac
      781 gacaacgcgc cagacactgc gaataccgaa ggactgcaat gaccgacgat cgctatcaat
      841 tgctgcggct gctcgaggcc atcctgtttg cctcgaccga acccttgggc gagaaggagc
      901 tggcgggccg catgcccgag ggcgccgaca tcaacggcct gttgcaggag ctgaaggatc
      961 agtacgccaa tcgcggcgtt cacctgctgg cgttcgagga tcgctgggcg ttccgcaccg
     1021 cgcccgatct tgcccccaag ttccatatcg agcgcgaggt cgtgcgcaag atgagccgcg
     1081 cggcgatcga gaccctcgcc atcatcgcct atcatcagcc ggtgacgcgg gcggagatcg
     1141 aggagatccg cggcgtcgtc atttccaagg gcacgctcga catgctgctg gaaatcggct
     1201 ggatcaagcc gaagggccgg cgtgagacgc cgggccgccc agtcacctgg gcgaccaccg
     1261 atgcgttcct ggagcatttc gggctggaaa gctgcgatgc gctgccgggc gtcgaggaac
     1321 tgaaggcggc cggcctgctc gatgcccgcg ccacggtcgc gaccctcggc acccagggcc
     1381 tgccgaatgg cgcgccggtg gccgaggagg aggacgaaga gggcaacatc tccgacatgc
     1441 tggcccagca gggcgaggac gaccccgagg cccaggcggc cgacgacgcc tatgatgccg
     1501 aggtcctgcg ccgggtcgag gcggacgagg aaggcgcgga gcccgacgag gaagacagcg
     1561 acgacgatct gtccgggtcc gccgagccca aggccgcggc gccttcggaa tagcggccca
     1621 ccgccgcagg tcagggcggc gaattgcaat tcagaataag tcgcattagg gtcggggcgt
     1681 gcccgccgac ggatcgccgg cgcccatgaa ccctttgcgc gggcctatcc cgccggacgg
     1741 aacgtcgctt gagcctgtcg caaccgcaag ccgccactcc ggttcgtttc ggtgcgcgcc
     1801 attggggctg gccgtcgcgc tggaccatcc ttgcctgcgg gctcgcgctg ctgatcgcgg
     1861 cgccggtgat ctcggtgctg atcaacgcct ttggcgcccg cagcgaaatc tggcagcacc
     1921 tggtcgagac ggtgctcggc gactacgtcg caaattcgtt cctgatcatg agcggcgtca
     1981 ccatcggcgt gctcatcgtc ggcgtgccgg cggcgtggat gacgaccttg tgccgctttc
     2041 cgggccggcg cctgttcgat tgggcgctgc tgctgccgat ggcctt
//
LOCUS       DATNBV010000101         7708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2922080,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000101 DATNBV010000000
VERSION     DATNBV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2922080"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..345
                     /gene="ykgO"
                     /locus_tag="VN627_02445"
     CDS             220..345
                     /gene="ykgO"
                     /locus_tag="VN627_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HXD02144.1"
                     /translation="MKIRNSLKSLKDRHRDNRVIRRRGRTYVINKTNRRFKARQG"
     gene            443..1054
                     /locus_tag="VN627_02450"
     CDS             443..1054
                     /locus_tag="VN627_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXD02145.1"
                     /translation="MADRVEAAVFDVGRVLVQWDLGALYRPLIPDPAECAWFLDNVVT
                     EQWHYQHDAGRPTAEMVAERTAEFPEHAHLIAAWAARFNETIPGPIPGTADLVEALHA
                     RGVPLYAITNFGAEFWPAFAPHWPVIGRFRDVVVSGVEKITKPDPRIYALAAQRFGHA
                     PATMLFVDDNATNIAAAQACGWQGHHFTDAETLAEDLKARGLI"
     gene            1087..1794
                     /locus_tag="VN627_02455"
     CDS             1087..1794
                     /locus_tag="VN627_02455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXD02146.1"
                     /translation="MNRRFALIAALATAALTATPLAAAPARKPAAARPLARPAPLGDT
                     VRVTMVTALGPIEFELDHAHAPVTVENFMRYVDSRRFDGMTFYRAMKLKWGEQPNGLI
                     QTGLRGNPAKVFKPIAHEPTSRTGIIHKAGTLSMARLAPGTATSDFSILLSDLTGLDA
                     DPASNDPELRAGYAAFGHVVGGMDVVRKIWNSPVSPTAGQGAMKGQLLAPEIKVLTVR
                     RSVMPVSAPAPAANAAP"
     gene            2020..2232
                     /locus_tag="VN627_02460"
     CDS             2020..2232
                     /locus_tag="VN627_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02147.1"
                     /translation="MSKFLTVACMSLACVGLAGCKVKQTKEAEMPEVHATGGQMPAYD
                     VKGPDVSVTTEPRTVEVPKVKVTPAQ"
     gene            2250..2525
                     /locus_tag="VN627_02465"
     CDS             2250..2525
                     /locus_tag="VN627_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02148.1"
                     /translation="MKALTRCATAAAMAALALTAATSAEARTCKRLNKTQGAVVGSVA
                     GGALGAVLTGGSTAGVLGGAAVGGVGGHEIARTKYNRHCRTYRRHRR"
     gene            complement(2611..4119)
                     /locus_tag="VN627_02470"
     CDS             complement(2611..4119)
                     /locus_tag="VN627_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HXD02149.1"
                     /translation="MVARVETVAYLGLEARTVEVQCQIAPGMPGFHLVGLPDKAVNES
                     RQRVQATLSTLGLALPPKRITINLSPADLPKEGSHYDLPIALALLAAMGVTDAEQLGD
                     WVAVGELALDGRIVPSPGVLLAALHASGADKGLICPAAQGAEARWASGIPILAAHDLI
                     ALLNHLKGVQVLVPPEPGEVQEQARGPDLRQVKGQEVARRALEIAAAGGHNLLMCGPP
                     GAGKSLLAACLPGILPPLAPAEALEVSMVASVAGTLEEGRISRTRPFRAPHHSASIAA
                     LTGGGLKVRPGEVSLAHLGVLFLDELPEFQRAALDSLRQPLETGTVTVARANAHVTFP
                     ARVQLVAAMNPCRCGHLGDPALGCSRAPRCAADYQSKVSGPLLDRIDLHVDVDPVRAA
                     DLALPPPSEGSAEVAARVAAARGVQVARQGETGARCNAELDGDVLERFATPDEAGRKL
                     LLQAAEAMRLSARGYTRILRVARTIADMAGAEAVGRIHVAEALSYRRMPARA"
     gene            4314..5168
                     /locus_tag="VN627_02475"
     CDS             4314..5168
                     /locus_tag="VN627_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXD02150.1"
                     /translation="MRRLFCLALGLAGLLATPAGAQQLAYAAMAPGAGPLATQVVTVT
                     EVTVEEFTEEDTSDGAAPGSDRFLPAAPAALPHGIATYGPFRVLDGTRAALVGATDER
                     SPAALRAMLAAHPGIATLELVECPGTEDDRANLVLGRMIRARGLATHVPAGGSVRSGA
                     VELYLAGLRHRAEPGAEFAVHAWADEDGREATDYPASAPENRAYLAYYREMGMTAAQA
                     SAFYAMTNSVPNASARWLTAAEMGQWVRSDRARATLTRISPSPNGPLLSGRPRGRPAM
                     YLFRRPVR"
     gene            5165..5299
                     /gene="rpmH"
                     /locus_tag="VN627_02480"
     CDS             5165..5299
                     /gene="rpmH"
                     /locus_tag="VN627_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXD02151.1"
                     /translation="MKRTFQPSNLVRKRRHGFRARTATVGGRNVLRARRARGRKKLSA
                     "
     gene            5328..5681
                     /gene="rnpA"
                     /locus_tag="VN627_02485"
     CDS             5328..5681
                     /gene="rnpA"
                     /locus_tag="VN627_02485"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXD02152.1"
                     /translation="MLTKRADFLAANRGLRVARPGFVLLARPNGGGQMRFGITVTKKI
                     GNAVVRNRMKRRFRVLLRELLPVHGLPGSDHVLIGREGGIERDFAKLREELVAALTRA
                     AAGKGDPPRPRKGKR"
     gene            5779..5988
                     /gene="yidD"
                     /locus_tag="VN627_02490"
     CDS             5779..5988
                     /gene="yidD"
                     /locus_tag="VN627_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXD02153.1"
                     /translation="MKRLLILIARAWQLGPSRVLPPSCRYAPSCSEYAIQALEKHGAI
                     KGGWLALKRLLRCHPWGGHGYDPVP"
     gene            6027..>7708
                     /gene="yidC"
                     /locus_tag="VN627_02495"
     CDS             6027..>7708
                     /gene="yidC"
                     /locus_tag="VN627_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXD02154.1"
                     /translation="MQNQRNLILAILLTILLIFGWDSAMRYMYPEANKPQNNAAGPAA
                     TQQAAPGADPARADKPTREGGLSNPADVALEARDLATALNPAGRVPIAAPGLSGSINL
                     TGALVDDLVTNRHTATIEKNSGPARIFSPAGTPAQQFAQVGWTGANVAVPNAQTVWTA
                     PAGARLTPKTPVTLTWTNGQGQTFAITYTVDEDYLLTIRQQVTNAAPAPVHLQPIAQI
                     NRTDKTASVSTWTLHSGPFGAFDGSVKFKPNYKDVVEAGTMANAGKPDWLGFTDIYWM
                     SVLAPDGAAQGSDFRALGNGIYLANLYYPAADVAPGQSLVHTTRLFAGAKESAVLDRY
                     EQAGLARFGHAIDWGWFFWFEKPIFWLLKALFKTVGNFGVAIILLTLIVRGIMFPVAQ
                     RQFASMAAMRAVQPKMKALQERFKDDKQRLQEETMKLYKEEGVNPLAGCLPIFLQIPV
                     FFALYKVLTLAVEMRHRPFALWIKDLSAPDPLHILNLFGLLHFTPPSFLGIGVLALLL
                     GVTMFFQFRLNPAQVDPVQQQMFAIMPWVMMFVMAPFAAGLLIYWITSNCLT"
BASE COUNT         1236 a         2837 c         2435 g         1200 t
ORIGIN      
        1 ggtcgcgcga ccggggaatg gtacgcatgt caggctccgt atctgaagct acgggctagt
       61 aatggtaagg ggcgaagtca aagaccctcc ggaaggatcg tgttgagatc tttcgtgaga
      121 ggtcaccggc gcgaggacat cggcgccttg actcagaccc taccacccac taacggcccg
      181 gcttccagtt ttccgcggca cccccgcacg aggaccatca tgaagattcg caacagcctg
      241 aagtcgctca aggaccgtca ccgggacaac cgcgtgatcc gccgccgcgg ccgcacctac
      301 gtcatcaaca agaccaaccg ccgcttcaag gcccgccagg gctgaagacc ggcggccggg
      361 cctcgcccgg tcggcacagc agcggcccgc ctccatcgga gcgcgggccg cagtcgtttg
      421 aggcgtcggt taggggcagg taatggctga tcgggtcgag gcggcggtct tcgatgtggg
      481 ccgggtgctg gtccagtggg accttggcgc gctctaccgt ccgctgatcc ccgatcccgc
      541 cgaatgcgcc tggttccttg acaacgtcgt caccgaacag tggcattatc agcacgacgc
      601 cggccggccg accgccgaaa tggtcgccga acggaccgcc gagttccccg aacacgcgca
      661 ccttatcgcg gcctgggcgg cccgcttcaa cgagacgatc cccggcccga tccccggcac
      721 ggccgatctc gtcgaggcgc tccacgcgcg cggcgtgccg ctctatgcga tcaccaattt
      781 cggcgcggaa ttctggcccg ccttcgcgcc ccactggccg gtgatcggcc gcttccggga
      841 tgtcgtcgtc tcgggcgtcg aaaagatcac caagcccgac ccgcgcatct atgccctggc
      901 cgcccagcgc ttcggtcacg cgcccgccac catgctgttc gttgacgaca acgccaccaa
      961 catcgccgcc gctcaagcct gcggctggca aggccaccac ttcaccgatg ccgaaacgct
     1021 ggccgaagac ctcaaggcac gcgggctgat ctagccaatg cgggggcctg gcggcatcct
     1081 gccaccatga accgccgatt cgccctcatt gccgccctgg ccaccgccgc gctcaccgca
     1141 accccgctgg ccgccgcccc cgcgcgcaag cctgccgccg cccggccgct ggccaggccc
     1201 gcccccctgg gcgacaccgt ccgcgtcacc atggtcactg cattgggccc gatcgagttc
     1261 gagctcgacc atgcccatgc gccggtcacg gtcgaaaact tcatgcgcta tgtcgacagc
     1321 cgccggtttg acgggatgac cttctaccgc gcgatgaagc tcaagtgggg cgagcagccc
     1381 aatggcctga tacagaccgg cctgcgcggc aatcccgcca aggtgttcaa gcccatcgcc
     1441 cacgagccga ccagccggac cggcatcatc cacaaggccg gcaccctgtc gatggcccgc
     1501 cttgcccccg gcacggcgac gtccgatttc tcgatcctcc tctccgacct gaccggcctc
     1561 gatgccgatc ccgcgtcgaa cgaccccgaa ctgcgcgcgg gctatgctgc cttcggtcac
     1621 gtcgtcggcg gcatggacgt ggtccgcaag atctggaatt ccccggtgtc gcccacggcc
     1681 gggcaagggg cgatgaaggg gcagttgctt gccccggaga tcaaggtgct cacagtgcgg
     1741 cgttcggtga tgccggtatc agcaccggca ccggcggcga atgcagcgcc ctaacgggcc
     1801 tccctgatgc ccgaaccgcg gcgttcgtat cccgatcgat ttggctgagc cttcattccg
     1861 agggccgtca gaactgcgcg aagctgcgag tttggcatcc acaattggta acaacgtcag
     1921 cggctatgcc cgccgcccgg ccgcgtccgg gaacagcctg accctcactg gagtttcgac
     1981 cacagctatt gtcgtgtcca aggtggcagg agaactaata tgtcgaaatt tcttacggtt
     2041 gcttgcatgt ctctggcctg cgttggtctg gccggctgca aggtcaagca gaccaaggaa
     2101 gccgagatgc ccgaggttca tgccaccggg gggcagatgc cggcgtacga cgtcaagggc
     2161 cccgacgtgt ctgtgaccac cgagccgaga acggtcgaag tgcccaaggt caaggtcacc
     2221 ccggcgcagt gaggacagga gcagtgacca tgaaagcttt gacccggtgc gcgacggccg
     2281 ccgcaatggc ggcccttgcg ctgaccgctg ccacctccgc cgaagcgcgc acctgcaagc
     2341 gcctgaacaa gacgcaaggc gccgttgtcg ggagtgtcgc cggcggggcc cttggcgcag
     2401 tcctgaccgg cggctcgacg gctggtgtgc tgggaggcgc agcagtgggc ggtgtcggcg
     2461 gacacgagat cgccaggacc aagtacaaca ggcactgccg gacctatcgc cgccaccggc
     2521 ggtgagttgc ggcagagtgt gaagtaaaga ggcgctccga gtggaccgga gcgtctcttt
     2581 ttcattgcgg cagccagccg ccgcgcacca tcacgcccgc gccggcatcc ggcgatagct
     2641 cagcgcctcg gccacatgaa tgcgtcccac ggcctccgcg cccgccatgt cggcaatggt
     2701 ccgcgccacg cgcaggatgc gggtatagcc gcgcgccgat aaccgcatcg cctcggccgc
     2761 ctgcagcagc agtttgcgcc cggcctcgtc gggcgtggcg aagcgctcca gcacgtcgcc
     2821 gtccagctcc gcattgcacc gcgcgccggt ttccccctgg cgggccacct gcacgccccg
     2881 cgccgccgcc acccgcgccg caacttcggc cgagccttcg ctgggcgggg gcagggcaag
     2941 atcggccgcg cgcaccgggt ccacgtccac atgcaggtcg atccggtcga gcagcggccc
     3001 ggaaaccttg ctctggtaat ccgccgcgca gcgaggcgcc cggctgcagc ccagcgccgg
     3061 atcgccgagg tgcccgcagc ggcacgggtt catagccgcg accaactgga ccctggccgg
     3121 aaaggtcaca tgggcattgg cccgcgccac ggtcaccgtc cccgtctcca gcggctggcg
     3181 cagcgaatcg agcgcggcgc gctggaactc gggcaattcg tcgaggaaca gcacgcccag
     3241 atgcgccagg ctgacctcgc cgggacgcac cttcagcccg ccgccggtca gcgccgcgat
     3301 cgaggcggaa tggtggggcg cgcggaatgg gcgggtgcgg ctgatccgcc cctcctccag
     3361 cgtgcccgcc accgaggcga ccatcgatac ctccagcgcc tcggccggcg ccagcggcgg
     3421 caggatgccc ggcaggcagg ccgcgagcag gctcttgccc gccccgggcg gtccgcacat
     3481 cagcaggttg tgcccgcccg ccgcggcgat ctccagcgcg cggcgggcca cctcctggcc
     3541 cttcacctgc cgcaggtccg gcccgcgcgc ctgctcctgc acctcgcccg gctcgggcgg
     3601 caccagcacc tgcacgccct tgaggtgatt gagcagcgcg atcaggtcat gcgcggccag
     3661 gatcgggatg ccgctcgccc agcgtgcctc ggccccttgc gcggcggggc agatcagccc
     3721 cttgtccgcc ccgctggcat ggagcgcggc cagcagcacg ccgggggagg ggacgatccg
     3781 cccgtcgagc gccagttcgc ccacggccac ccagtccccc agctgctcgg catcggtcac
     3841 ccccatggcg gcgagcaggg ccagggcaat cggcaggtcg tagtgcgaac cctccttggg
     3901 caggtcggcc ggggaaaggt tgatcgtgat ccgcttggga ggcagggcca gccccagcgt
     3961 gctcagcgtg gcctggaccc gctggcggct ttcgttcacc gccttgtcgg gcaggccgac
     4021 gaggtgaaac cccggcatcc ccggcgcgat ctggcactgc acttcgaccg tgcgcgcctc
     4081 cagcccgaga taggccaccg tctccacccg cgcgaccacg cgccgtcccc cctgaaactg
     4141 cctaaggaaa agcccctatc ctgccgtgat ggatcagcct tccgccggtg tcgagagggc
     4201 gcggcacaat cgattccatt tcggacctgt cacctgctcc ttaacccgtc gtcagcaaat
     4261 cttaaccgag cccatcagca aggcggcaag gggcggggtc gcatgaacag gcgatgcggc
     4321 gcctgttctg ccttgccctc ggtcttgccg gcctgctcgc caccccggcg ggcgcgcagc
     4381 agctcgccta tgccgccatg gcgcccggcg ctggcccgct cgctacccag gtggtgaccg
     4441 taaccgaagt gacggtcgag gaattcaccg aggaagatac ctccgacggc gccgcgccag
     4501 gctccgaccg cttcctcccc gccgcccccg ccgccctccc gcacggcatc gcgacctacg
     4561 gcccgttccg cgtgctggac ggcacccgcg ccgcgctggt cggcgctacg gacgagcgca
     4621 gccccgccgc cttgcgcgcc atgctcgccg cccatcccgg cattgccacg ctcgagctgg
     4681 tcgaatgccc cggcaccgag gatgaccgcg ccaatctcgt attgggccga atgatccggg
     4741 cgaggggcct tgccacccac gtccccgccg gcggctcggt tcgctccggc gcggtcgagc
     4801 tttatcttgc cggcctgcgc caccgggccg agcccggcgc ggagttcgcc gtccacgcct
     4861 gggccgacga agatgggcgc gaggccaccg actatcccgc ttctgccccg gaaaaccgcg
     4921 cctaccttgc ctactaccgc gaaatgggca tgacggcggc gcaggcgagc gccttctacg
     4981 ccatgaccaa ttccgtcccc aatgcctcgg cccgctggct caccgccgcc gagatgggcc
     5041 aatgggtgcg gagcgataga gcaagggcga ccttgactcg aatctctccc tcgcctaacg
     5101 ggccgcttct ttccggtcgt ccgcgcgggc ggcctgcaat gtatctattt cgaaggccag
     5161 tcagatgaag cgcacttttc agccgagcaa cctcgtccgc aagcgtcgcc acggtttccg
     5221 tgcccgcacc gcgaccgtgg gtggccgcaa cgtgctgcgc gcccgccgcg cccgcggccg
     5281 gaagaagctc tctgcctgag gaagctcccg cggggcctgc cctcgcggtg ctgacgaagc
     5341 gggccgattt cctcgccgcg aatcgcggct tgcgcgtcgc ccgccccggt ttcgtcctgc
     5401 ttgcccgccc caatggaggc gggcaaatgc gtttcgggat caccgttacc aagaagatcg
     5461 gcaacgccgt cgtccgcaac cggatgaagc gccgctttcg cgtcctgctg cgcgagctcc
     5521 tgccggtcca tggcctgccg ggctcggatc atgtcctgat cggccgcgaa ggcgggatcg
     5581 agcgcgattt tgccaagctg cgcgaagaac tcgtcgccgc gctcacccgg gcggcggcgg
     5641 ggaagggcga tccgcctcgg ccgagaaagg gcaaacgctg atgccggccg catttcaccc
     5701 ctccttcagg cgggaggggc agcgagacct gggtgcgacg cgccttaagc gcagcggggc
     5761 gggcttctgc gccagccaat gaaacgcctc ctcatcctca tcgcccgcgc ctggcagctc
     5821 ggcccctcgc gggtgcttcc gccctcctgc cgctatgcgc cctcgtgctc ggaatacgcg
     5881 attcaggcgc tcgaaaagca cggggcgatt aagggtggat ggctggcatt gaagcgtcta
     5941 ttgcgctgcc acccgtgggg tgggcacggc tacgatccgg tcccctgaac cctgcaacag
     6001 atctagaaag acgagcggca aactccgtgc agaaccagcg caacctgatc ctggccatat
     6061 tgctgaccat cctgctgatt ttcggctggg acagcgccat gcgctacatg taccccgagg
     6121 cgaacaagcc ccagaataat gctgcggggc ctgccgccac gcagcaggcc gcgcccggcg
     6181 ccgatccggc gcgggccgac aagcccaccc gagagggcgg gctgtccaac ccggccgatg
     6241 tcgcgctgga ggcgagggac ctcgccaccg cgctgaaccc ggccggccgc gtgcccatcg
     6301 ccgcgccggg cctctccggc tcgatcaacc tcaccggcgc cctggtcgat gatctcgtca
     6361 ccaaccgcca caccgccacg atcgagaaga actccggccc ggcccggatc ttctcgcccg
     6421 ccggcacgcc tgcccagcag ttcgcgcagg tcggttggac cggcgctaac gtcgcggtcc
     6481 ccaatgcgca gaccgtgtgg accgcgcccg ccggcgccag gctgacgccg aagacgcccg
     6541 tcactctcac ctggaccaac ggccagggcc agaccttcgc gatcacctac acggttgacg
     6601 aggactacct gctcaccatc cgccagcagg tcaccaacgc cgccccggcg ccggtccatc
     6661 tccagccgat cgcccagatc aaccgcacgg acaagaccgc cagcgtctcc acctggaccc
     6721 tgcattccgg ccccttcggc gccttcgacg gctcggtgaa attcaagccg aactacaagg
     6781 acgtggtcga ggccggcacc atggccaatg cgggcaagcc cgattggctc ggcttcactg
     6841 acatctactg gatgagcgtc ctcgccccgg acggcgccgc gcaggggtcc gacttccgcg
     6901 cgctcggcaa cggcatctat ctcgccaacc tctattatcc ggctgccgac gtggccccgg
     6961 gccagagcct cgttcacacc accaggctct tcgccggcgc caaggaatcg gccgtgctcg
     7021 accgttacga gcaggccggc ctcgccaggt tcggccatgc gatcgactgg ggctggttct
     7081 tctggttcga gaagccgatc ttctggctgc tgaaggccct gttcaagacc gtcggcaatt
     7141 tcggcgtcgc catcatcctg ctgaccctga tcgtgcgcgg gatcatgttc ccggtcgccc
     7201 agcgccagtt cgcctcgatg gccgcaatgc gcgccgtcca gccgaagatg aaggccctcc
     7261 aggaacgctt caaggacgac aagcagcgcc tccaggaaga gacgatgaag ctctacaagg
     7321 aggaaggggt gaacccgctg gccgggtgcc tgcccatctt cctgcagatc ccggtgttct
     7381 tcgcgctcta caaggtgctg accctcgcgg tcgagatgcg ccatcggccc ttcgcgctgt
     7441 ggatcaagga tctttccgcg cccgatccgc tgcacatcct caacctgttc ggcctgctgc
     7501 attttacgcc gccctcgttc ctcggcatcg gcgtgcttgc gctgctgctg ggcgtgacga
     7561 tgttcttcca gttccggctg aacccggcgc aggtggaccc ggtgcagcag cagatgttcg
     7621 cgatcatgcc ctgggtcatg atgttcgtca tggcgccctt tgccgcgggc ctgctgatct
     7681 actggatcac ttcgaactgc ctgacgat
//
LOCUS       DATNBV010000102         2655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_699393,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000102 DATNBV010000000
VERSION     DATNBV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699393"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VN627_02500"
     CDS             complement(<1..403)
                     /locus_tag="VN627_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02155.1"
                     /translation="MPERIGGTLPPPGLLAELLDVVAGGVDVGSGVDVGVAVMIGGTL
                     PPPELLFVVAIGPVEDGTAAGVAVITGGSFPPDWGWTEMVPPWLLLELVAVVPPDWEL
                     LAFVPPDWKLVAAMPPKLAPSPGCLVPLELVA"
     gene            918..2225
                     /locus_tag="VN627_02505"
     CDS             918..2225
                     /locus_tag="VN627_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD02156.1"
                     /translation="MARRAGYTDQSLTKFAIVIAHISLKWSPAENAMHATQDVKSDVK
                     TIAPAPADLVARARALPLTLAARAAKAEQDRRVPHDTIADMQAAGLFRVFQPKRWGGY
                     EMDPATYFEIQLALAEGCMSTGWVYGVVGLHPWLMGLFDDRAAQEVWGTDDTTLICSS
                     LMPTGTAVSAPGGLRLSGRWKFSSGSEHCGWAYLGGTPAEDGKPGDRSIFLVPRSDYE
                     IEDTWRVSGLKATGSHDIVVKGAFVPDYRRLRWIDNFNCVGPGQSVNTSVLYRWIFGQ
                     IFFRGVSTPALGALQGMLDAYLDYGKKRIQRNSGVAASEEAVIQLLCAETAVAIDEMK
                     TILHRNFKVLEGYAARGEMPPLKQRVEYKFHNAVVAERCSLLASRLFKAAGTAGIAAD
                     LPFGRILNDITVGRQHISNQFEQAGKSYGAFLFGIENNKDFVL"
     gene            2319..>2655
                     /locus_tag="VN627_02510"
     CDS             2319..>2655
                     /locus_tag="VN627_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02157.1"
                     /translation="MKLCRYDDDRLGVVIGDKVHDVTQAQTEIRNSTPYTAKVDPVVA
                     ALPKWRDKLEQMAKAAPGKPVSQVKLLPPVARPPKIMAAPTNYAKHVEEMQKFRDTVP
                     GLARFSPDIE"
BASE COUNT          502 a          885 c          814 g          454 t
ORIGIN      
        1 gtgccaccag ctccaacggc accaagcagc ccggcgacgg ggctagcttt ggcggcatag
       61 ctgccaccag cttccagtca ggcggcacaa aggccagcag ctcccagtcg ggcggcacaa
      121 cggccaccag ctccagcagc agccaaggtg gcaccatctc ggtccaaccc cagtcaggcg
      181 ggaagctgcc tccggttatc actgcgactc cggccgcggt cccatcttcg accgggccaa
      241 tcgcgaccac gaacaacaac tccggcggcg gcagggtgcc tccaatcatc actgcgaccc
      301 cgacgtcgac cccgctcccg acgtcgaccc cgccggcgac cacgtcgagc agctccgcca
      361 gcaatcctgg tggcggcagg gtgcctccga tcctctccgg cattccgact ccgatcccgc
      421 aagcaaccgg gccgatcgcc aacaatcccg gcggtgccaa ggtccctccg atcctctctg
      481 ggactccgac atcgacgccg atcccgccgg ccacggggcc gatcaaccct ggcgacttgc
      541 ccggcagcag gcctccgatc ttcactccga ctccggcccc gatcccgtcg ggcaccgggt
      601 cgctcccgaa tccgggcaac tatcctggca ctcctccgat ctccacggtg ccgatcccga
      661 cccctgtccc aattccggtc cccaccggat cgagtccgag ccccaacccc gggtatggga
      721 acggcacaag ctacacgcat cagcgcggcc aatatggcgt ctacgctgat atcggcgccg
      781 atggcggcgg tcacgaaccg tgctggttgt tcaagagcaa ctacgacaga acgggcagtc
      841 cttattggct caacaggtac aaggtctgtc tcgggctgca ttaatcccga ctgagttgga
      901 ccagcacgac gggcggcgtg gcccgtcgtg ctggatacac cgatcaaagt ttgacgaaat
      961 tcgctattgt cattgcccac atttccttga aatggtcgcc ggcggaaaat gccatgcacg
     1021 cgacccaaga tgtgaagagc gatgtgaaga ccattgcgcc cgcgccagca gatctggttg
     1081 cgcgcgctcg tgcgctccca ttgacgctcg cggcgcgcgc cgccaaggct gagcaagatc
     1141 ggcgcgtacc gcacgacacc atcgcagaca tgcaggcggc cggattgttt cgcgtgttcc
     1201 agcccaagcg ctggggcggc tacgagatgg acccggcgac ttacttcgag atccagctcg
     1261 cgctcgcgga aggctgcatg tcgacgggtt gggtctatgg cgtcgtcgga ttgcatcctt
     1321 ggctgatggg cctgttcgac gaccgtgccg cgcaggaggt gtggggcaca gatgacacga
     1381 ccttgatctg ctcctcgctg atgccaaccg gtacagctgt ttcggcgccc ggcggccttc
     1441 gcctttctgg gcgctggaag ttttccagcg gcagcgagca ctgcggctgg gcctatctcg
     1501 gcggcacgcc cgcggaggat ggcaagccgg gcgatcgcag catctttctc gtgccgcgca
     1561 gcgactacga gatcgaggac acctggcgtg tgtcagggct gaaggcgacc ggcagccacg
     1621 acatcgtggt gaaaggcgcc ttcgtgcccg actatcgtcg tctgcgctgg atcgacaatt
     1681 tcaattgcgt cggtcctggc cagtcggtca acacgtcagt gctctatcgc tggatcttcg
     1741 gccagatatt cttccgcggc gtctcgacgc ccgccttggg cgcgctgcaa ggcatgctcg
     1801 acgcgtatct cgactacggc aagaagcgca tccagcgaaa ctccggggtg gcggcgtcgg
     1861 aggaggccgt gatccagttg ctttgcgccg aaaccgccgt ggcgatcgac gagatgaaga
     1921 ccatcctgca ccgcaacttc aaggtgctgg aaggctatgc cgcgcgcggc gagatgccac
     1981 cgctgaagca acgcgtcgaa tataaattcc acaatgccgt ggtggcggag cgctgcagtc
     2041 tgctggcgtc gcggctgttc aaggcggccg gcactgccgg catagccgct gatctgccgt
     2101 ttgggcggat tctcaacgat atcaccgttg gccgtcagca catttccaat cagttcgagc
     2161 aggccggcaa gagctatggc gcgttcctgt tcggcatcga gaacaacaag gactttgtgc
     2221 tctaacgggc gtgccaacac tggccaagat cggccaaaat cggctatgtc cgcgctggaa
     2281 ttgacgtcat gcgctgaggc gcccgtgggg agtagaacat gaagctctgt cgatacgacg
     2341 atgaccggct cggcgtggtg atcggggaca aggtccacga cgtaacccag gcgcagaccg
     2401 agatcaggaa ttcgaccccc tacacggcga aggtcgaccc ggtggtggcg gcgctgccga
     2461 aatggcgcga caagctcgag cagatggcca aggccgcgcc cggcaagccc gtctcgcagg
     2521 tcaagcttct gcctcccgta gcccggccgc cgaagatcat ggcggcgccg accaactacg
     2581 ctaagcacgt cgaagagatg cagaagttcc gcgacactgt tccgggcctg gcgcgattct
     2641 cgcccgacat cgaga
//
LOCUS       DATNBV010000103         4429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_76506,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000103 DATNBV010000000
VERSION     DATNBV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76506"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VN627_02515"
     CDS             <1..269
                     /locus_tag="VN627_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HXD02158.1"
                     /translation="GTDSAVISGRKAQLQAWKREFGAADRTVYRRVPDRVEISPVAPL
                     AMEHGRWEGVSLSGIALASGTYAAKWRRLGADWVIEAEIFLTTG"
     gene            complement(467..2014)
                     /locus_tag="VN627_02520"
     CDS             complement(467..2014)
                     /locus_tag="VN627_02520"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HXD02159.1"
                     /translation="MRYAYGITSALLLGGAALSLATGLPAGAQVAQNDAQQIQQVVPR
                     AGAPASFADLTAQLQPAVVNISTRQRVRVAQQGNPFAGTPFADLFGGDQAQGGTPQTR
                     EAQSLGSGFLISADGYVVTNNHVITADGQGEVESITVKTNDGTEYPAKLVGRDAASDL
                     AVLKITAPKPFPFVRFGDSRNARVGDWVIAIGQPFGLGGTVTAGIISAVYRATGSGSA
                     YDRYLQTDAAINRGNSGGPMFDMRGQVIGINNAIFSPTGGSVGIGFAIPAETAAPIVE
                     KLKTGQAIERGYLGVSIQQLNEDLADSLGIPHNRGEFIQAVVPGGAGARAGLQPGDVV
                     LRVDGKEVTPDQTLSFIVANTPPGKRIPLELIRNGQRLTVSAVVGKRPTEEQLAQDQS
                     FGSNDQDNSDNAYNQTPQKQGQGVVEQSLGISAIPLTPTIARQLGQSEGTQGVVVTAV
                     DASSDAGQKGLQRGDIVLSANYQPVTTVAQFEAVVRAAKAQNRPAVLLRVQRRGQPAT
                     YVPIRLR"
     gene            complement(2205..3068)
                     /locus_tag="VN627_02525"
     CDS             complement(2205..3068)
                     /locus_tag="VN627_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HXD02160.1"
                     /translation="MSPSALWQENKAAVAAAIAGLLVLLSCVTIVPENEQAVIVRVGE
                     PVRVINRFRPGADFGSTGAGLIFRVPIVERLVRIDKRVLSVDMDRQQVLSTDQQRIEV
                     DAYARFRIIDPVRMVRSAGTTERVAEQLQPILSSVLRQELGKRTFQSLLTAERGQAMI
                     NIRKGLDREAREYGAQVIDVRIKRADLPDGTPLESAFARMAAAREQEATTIVAQGQKN
                     AQIIRATAEANAAKVYADAFGKDPAFYDFYRAMQSYDTTFSNPKNAGQSTILLSPDSE
                     YLKQFKGKIGN"
     gene            complement(3068..4276)
                     /locus_tag="VN627_02530"
     CDS             complement(3068..4276)
                     /locus_tag="VN627_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HXD02161.1"
                     /translation="MKSIGDAIARGWLAATGGNDGEKPKGGKSAPSPWGSGDGSANGP
                     ADEPVEPARGADEPEDKPKAPGPRNPWLPGSSAPGRPSGTGGDGARRGPSLDELFRGR
                     PGGGGGFGGGRFPGIPQRPDGRSWLPLIAAGVVAVWLLSTTTHMLGPKEQGIVTTLGK
                     YSRTIEPGVSWTLPWPIENVRVEDVTSIKRDTIPEGEAEKLMLTSDQNLVDISYLVRW
                     NIKDLKLYSFQLADPDNTVKEVAEAAMRASIAEVPLAEAMGGTGRGDIEQAVRTRMQA
                     VLDAYRSGVLIQGVEIKKADPPAKVIDAFQKVTAAQQDAQREVSNAQAWAQQITQRAQ
                     GDATAFDKVYEQYKLAPEVTRRRMYYETMERVLSANEKVIVAAPGVTSYLPLPELKRK
                     AGAAAEGGNQ"
BASE COUNT          668 a         1383 c         1609 g          769 t
ORIGIN      
        1 cagggacgga cagcgcggtg atttccgggc gcaaggcgca gctgcaggca tggaaacgcg
       61 aattcggggc ggcggaccgg acggtgtatc ggcgggtgcc ggaccgggtg gagatttcgc
      121 cagtggcgcc gctcgccatg gaacatgggc gctgggaggg tgtgagcctg tccggcatcg
      181 cgctggcgag cgggacctat gccgccaagt ggcgccgcct gggagcggac tgggtgatcg
      241 aggcggagat tttcctgacg accgggtgag gggcgagagg cgtctgttac gcggaaatac
      301 gcgtcggcac ctgcccttcg ggtagccagg gctccatccc aagttcgcgg tctgcaccag
      361 cagatgccga tcagagcgag gccgggatag gcccctgcct atgcagaggc gacggttttc
      421 tgagaccggc agatgccctc tccccgcagg gagagggcca atcggttcag cgcaggcgga
      481 tcggtacgta ggtggcgggc tggccgcggc gctggacgcg caggaggacg gccggacggt
      541 tctgggcctt ggccgcgcgg accacggctt cgaactgcgc gacagtggtc accggctggt
      601 aattggccga gaggacgatg tcgccacgct gaaggccctt ctggccggca tcggacgagg
      661 cgtcgaccgc ggtgacgacc acgccttggg tgccttcgga ctggccaagc tggcgcgcga
      721 tggtcggcgt cagcgggatc gcggagatgc cgagcgactg ttcgaccacg ccctggccct
      781 gcttctgcgg ggtctggttg taggcgttgt cgctgttgtc ctggtcgttc gagccgaagg
      841 actggtcctg cgccaattgc tcttcggtag gccgcttgcc gacgacggcg ctgacggtca
      901 ggcgctggcc gttgcggatc agttcaagcg ggatacgctt gcccggaggg gtgttggcga
      961 cgatgaacga cagggtctgg tcgggcgtga cctccttgcc gtcgacgcgc aggaccacat
     1021 cgcctggctg gagcccggcg cgggcgccgg cgccgcccgg gacgaccgcc tggatgaatt
     1081 cgccgcggtt gtgcgggatg ccgagactgt cggcgaggtc ctcattgagc tgctggatgc
     1141 tcacgccaag atagccgcgt tcgatggcct ggccggtctt gagtttttcg acgatggggg
     1201 cggcggtttc cgccggaatg gcgaagccga tgccgaccga gcctccggtg ggcgagaaga
     1261 tcgcattgtt gatgccgatc acttgcccgc gcatgtcgaa catcggcccg ccggaattgc
     1321 cgcggttgat cgcggcgtcg gtctgcaagt agcgatcgta ggccgaacct gagccggtgg
     1381 cgcggtagac cgccgagatg atccccgccg tgaccgtgcc gccgaggccg aagggctggc
     1441 cgatggcgat cacccagtcg cccacccgcg cattgcggct atccccgaac ctgacgaagg
     1501 ggaagggctt gggcgcggtg atcttgagca cggcaagatc cgacgcggca tcgcggccga
     1561 ccagcttggc cggatattcg gtgccgtcgt tggtcttgac tgtgatcgat tcgacctcgc
     1621 cctggccatc ggcggtgatc acgtgattgt tggtgaccac atagccatcg gccgagatca
     1681 ggaagcccga gccgagcgac tgggcctcgc gcgtctgcgg ggtgcccccc tgggcctgat
     1741 cgcccccgaa caggtcggcg aagggcgttc cggcgaaggg gttcccctgc tgggcgacgc
     1801 ggacgcgctg gcgggtggag atgttgacca cggccggctg gagctgggcg gtcaggtcgg
     1861 caaaactggc aggggctccc gcgcggggga caacctgctg gatctgctgc gcatcgttct
     1921 gcgcgacctg ggccccggcg ggcaggccgg tggcaaggct gagggccgcg ccgccgagca
     1981 gcagcgccga agtgattcca taagcgtaac gcacgggctt cacgtcctct gtattccttt
     2041 gcctgggtgc gcgtggcggc gcaccgggtc gatcatgagc gccgatacaa gcaccttcgg
     2101 cttaacccac attgaatgac gctgttgtag gtgcgcactt ggcatccgcc aagggctggt
     2161 accgcaaagg aacggccgga gggccattcc gcacccgaca aatactaatt cccgatcttg
     2221 cccttgaact gcttcaggta ttccgaatcc ggtgaaagca ggatcgtgct ttgcccggcg
     2281 ttcttggggt tggagaaggt cgtgtcgtag ctctgcatcg cgcggtagaa gtcgtagaag
     2341 gccgggtcct tgccgaaggc atcggcatag accttggcgg cattggcctc ggccgtggcg
     2401 cgaatgatct gggcgttctt ctgaccttgc gccacgatgg tggtcgcctc ctgctcgcgc
     2461 gcggcggcca tgcgggcgaa ggcggattcg agcggggtgc cgtccggcag gtcggcccgc
     2521 ttgatgcgta cgtcgatcac ctgggcgcca tattcacgcg cctcgcggtc aagccccttg
     2581 cggatgttga tcatcgcctg gccccgctcg gcggtgagca gcgactggaa ggtgcgcttg
     2641 cccagttcct ggcgcaggac cgaggagagg atcggctgga gctgttcggc cacgcgctcg
     2701 gtcgtcccgg cggaacggac catgcggacc ggatcgatga tgcggaagcg ggcataggca
     2761 tccacctcga tgcgctgctg gtcggtcgag aggacctgct ggcggtccat gtcgaccgag
     2821 agcacgcgct tgtcgatccg caccagccgc tcgacgatcg gcacgcggaa gatgagcccc
     2881 gcgccggtcg acccgaaatc ggcgccgggg cggaagcggt tgatcacgcg gaccggctcg
     2941 ccgacgcgga cgatcaccgc ctgttcgttt tccggcacga tggtgacgca ggagagcagg
     3001 acgaggaggc cggcgatcgc ggcggccacg gcggccttgt tttcctgcca gagggcgctg
     3061 ggcgacatta ctggttcccc ccttcggcgg cagcgccggc cttgcgcttg agttccggca
     3121 gcggaaggta ggaggtgacc ccgggcgcgg ccacaatcac cttttcattg gcggagagca
     3181 cgcgctccat cgtttcatag tacatgcggc ggcgggttac ctcgggggcg agcttgtact
     3241 gctcgtagac cttgtcgaaa gcggtggcat cgccctgggc gcgctgggtg atctgctggg
     3301 cccaggcctg ggcgttggaa acctcgcgct gggcatcctg ctgcgcggcg gtgaccttct
     3361 ggaaggcatc gatcaccttg gccggcggat cggccttctt gatctccacg ccctggatca
     3421 gcacgcccga gcggtaggcg tcgagcacgg cctgcatgcg ggtgcgcacc gcctgctcga
     3481 tatcgccgcg gccggtgccg cccatggctt cggccagcgg cacttcggca atcgaggcgc
     3541 gcatggcggc ctcagccact tccttcaccg tgttgtccgg atcggcgagc tggaaggagt
     3601 agagcttgag gtccttgatg ttccagcgca cgagatagga gatgtcgacc aggttctggt
     3661 cgctcgtcag catgagcttc tcggcctcgc cctcggggat cgtgtcgcgc ttgatcgagg
     3721 tgacgtcctc gacccgcacg ttctcgatgg gccagggcag ggtccacgag acgcccggct
     3781 caatcgtgcg cgaatacttg cccagggtgg tgacgatgcc ctgttccttg ggcccgagca
     3841 tgtgggtggt ggtggacagc agccagaccg cgaccacgcc ggcggcgatc agcgggagcc
     3901 acgagcgccc gtcgggccgc tgcgggatgc cggggaagcg gccgccgccg aagcccccgc
     3961 cgccgcccgg gcggccacgg aacagttcat cgaggctggg gccgcgacga gcgccgtcgc
     4021 cgccggtacc cgaggggcga ccgggcgcgg aactgcctgg cagccacgga ttgcgggggc
     4081 cgggggcctt gggcttgtcc tccggctcat cggcgccgcg ggccggttcg accggttcgt
     4141 cggccgggcc attggccgaa ccgtcacccg agccccatgg gctgggcgcg gatttgccgc
     4201 ccttcggctt ttcgccgtcg ttcccccccg tcgcagccag ccagccgcgc gcgattgcat
     4261 cgccgattga tttcatgagc gccgttatag gaacggcccg cgcacaaaaa cagtgcccgc
     4321 tcgcgcattt tccttgctag gggctctggc cagcgaattg gagtgcctgg accggacatg
     4381 agcgtcgacc tcgaagccct gaagaacctg ctgccggacg atctcaagg
//
LOCUS       DATNBV010000104         3335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_76874,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000104 DATNBV010000000
VERSION     DATNBV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76874"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VN627_02535"
     CDS             complement(<1..188)
                     /locus_tag="VN627_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02162.1"
                     /translation="MAGRQSGLIGNSRKTGLACLLAGIAVAALAAAPALAAGDPAPAA
                     PPASAPLPRDYPAIPQAP"
     gene            273..1181
                     /locus_tag="VN627_02540"
     CDS             273..1181
                     /locus_tag="VN627_02540"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXD02163.1"
                     /translation="MSLANVDLRKLHHALVLADCGGFTAAAARLNLTQSALSRSVQSL
                     EQQLGLVIFDRGRNGARPTMAGREFLARARDIQGAMLHLERFAADLGRGAEGEVTVGL
                     GTSVCHAVLARVVGRIAAEHPRLSLTVKSEPVQALIDDLANGGCDLVFVADTHLIDRS
                     RVAASAVIPAQAGVLVRGDHPLAAAGAVSLDDLSGYTFVTGPVDPNEVRAIFGRDCRL
                     LTCDDPRLLRDLVLAGDALWLTQTFVAADDLAAGRMAMLTLADRRARSRFTIYELAMR
                     QTRLSRSAEIVAGLVRQELTERAARL"
     gene            complement(1192..2433)
                     /locus_tag="VN627_02545"
     CDS             complement(1192..2433)
                     /locus_tag="VN627_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXD02164.1"
                     /translation="MASEHRLGFDTFKSTDPDPWHQIRRDRERCPVVHVEAANGFSFL
                     QVGTYDLVREVLRQPEVFSNRMGVIPRGAEGDEEQVLEFADPPEHTIHRALIGKAFSA
                     ARVNERHARIQAVADDLVDEIAEAGNGFQLRARFGRQLPSRIISEILGVPDEDRARFI
                     EWSEIGEAAVGQLEPDSGTVKAQEAFLEYCRKCLRDRLNSPRDDLLSTIIHAQQDGQR
                     FTETEAAAMVRLLLAAGNGTTSIGISNLVYLLETNPDEKRRLLADMDGLIASAVEEGF
                     RYDCPVQGNLRGVLRETEVGGHKLMPGDRAYLFYTSANHDPAHYDEPDRFRIDRDWTR
                     EARHYAFGFGIHFCLGAELARAETQIAIRTLYTRLPNLRMKAGFRPQQVPGMVFRTWA
                     EIEMEYDGAPLPRLSESSGAG"
     gene            complement(2437..>3335)
                     /locus_tag="VN627_02550"
     CDS             complement(2437..>3335)
                     /locus_tag="VN627_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXD02165.1"
                     /translation="PGRPPAVTAMSLQTLNHLTGGNRIIAGVGTAALQAREGFQGLPW
                     GKPVRRMRDFVTILRQGLEGQPLDHDGQEWCAPYRGPGAHGLPPAPLGLAPMGEIPVC
                     IAASGPQMIALAAEIGDGWMPPGWHIGDYQMFRPLLDQGFARRPGGEPKDYKIWAHVD
                     MLVDDDVRAAMRPFKEYVVTWSAIHREFMIARGFKDVADRLAELVAQDPGADPEARLQ
                     AGEKFLRDPLWEEAIAAVPDEYIDNGWLVGPVARIEERARRWRDCGLSGMIVRYGDQL
                     SHEPIVENLDAFRAVARAFGRA"
BASE COUNT          498 a         1098 c         1178 g          561 t
ORIGIN      
        1 ttgggggcct gcgggatcgc cggatagtcg cgcggaagcg gggcgctggc ggggggagca
       61 gcaggggcgg gatcgcccgc tgccaaggcc ggagcggccg cgagggctgc cacggcgatg
      121 ccggccagga ggcaggccag acccgtcttg cgcgaattgc cgatgaggcc cgattgccgc
      181 ccagccatgc ctgtctcccg gtctgatttt ggtgttgctt gtgaatgtca ggcacgacac
      241 atggtgtcca atgcatattg ggacccgttt ccatgagttt ggcgaatgtt gacctgcgca
      301 agctgcacca tgccctggtc cttgcggact gcggcggctt tacggccgct gccgcccggc
      361 tcaacctcac ccaatcggcg ctgagccgca gcgtgcagtc gcttgagcag cagctggggc
      421 tggtgatctt cgaccgtggc cgcaatggcg cgcggccgac catggcgggg cgcgaattcc
      481 ttgcccgcgc ccgcgatatc cagggcgcca tgctgcacct tgagcggttt gccgccgatc
      541 tcgggcgcgg cgcggagggg gaggtcaccg tgggccttgg cacttcggtg tgccacgcgg
      601 tcctcgcgcg ggtggtcggg cgcatcgccg ccgagcatcc gcgcctgtcg ctgacggtga
      661 aatccgagcc tgtccaggcg ctgatcgacg atctcgccaa cggcggctgc gatcttgtct
      721 tcgtggccga tacccatctg atcgatcgca gccgggttgc cgccagcgcg gtcatcccgg
      781 cgcaggcggg cgtgctggtg cgcggcgatc atccgctggc ggccgcgggc gccgtatcgc
      841 tggatgactt gtccggatac accttcgtta ccggcccggt ggacccgaac gaggtgcgcg
      901 ccatcttcgg acgggactgc cgcctgctca cctgcgacga cccgcgcctg ctgcgcgacc
      961 tcgtgctggc gggcgatgcc ctatggctga cccagacctt cgtcgcggcc gacgacctcg
     1021 ccgccggccg catggcgatg ctcacgctgg ccgaccggcg cgcccgctca cgcttcacca
     1081 tttatgagct ggcgatgcgc cagacgcgcc tttcgcgttc ggcggaaatc gtcgccgggc
     1141 tggtgcggca ggaactgacc gagcgggcgg caaggctcta ggcagggacg gtcatccggc
     1201 gccgctgctc tcggacaggc gcggcagcgg cgcgccgtcg tattccatct cgatctcagc
     1261 ccaggtgcgg aagaccatgc cgggcacctg ctgcggccga aagccggctt tcatcctgag
     1321 attgggcaac cgggtataga gggttcggat cgcgatttgt gtctctgcgc gggcaagttc
     1381 ggcgccgagg caaaaatgga tgccgaagcc gaaggcatag tggcgcgcct cgcgggtcca
     1441 atcgcgatcg atgcggaacc ggtcgggctc atcatagtgc gccggatcgt gattggccga
     1501 ggtgtagaac aggtaggccc ggtcgcccgg catcagcttg tgaccgccga cttcggtttc
     1561 acgcaggact ccccgcaggt ttccctgcac cggacagtcg tagcggaacc cttcttccac
     1621 ggccgaggcg atcagcccgt ccatgtcggc cagcaggcgg cgcttctcgt caggattggt
     1681 ctcgagcaga tagaccagat tggaaatgcc gatcgaagtg gtgccgtttc cggcggccag
     1741 cagcaggcga accatggcgg cggcctccgt ctcagtgaag cgttggccgt cctgctgggc
     1801 atgaatgatc gtgctgagca gatcgtcccg cggcgaattc aggcgatcgc gcaggcactt
     1861 gcggcaatat tcgaggaagg cctcctgcgc cttgactgtg cccgaatcag gttcgagctg
     1921 gccgaccgcg gcttccccga tctccgacca ttcgatgaac cgggcgcgat cttcgtcggg
     1981 tacgccaagg atttccgaga tgatgcgcga aggcagctgc cggccgaacc gggctctcag
     2041 ctggaacccg ttgccggctt cagcgatctc gtcgaccagg tcgtccgcca cggcctggat
     2101 gcgggcgtgc cgctcgttga cccgggcggc ggagaaagcc ttgccgatca gcgcgcggtg
     2161 gatggtatgc tctggcgggt cggcaaattc gagcacctgt tcctcgtcgc cttccgcgcc
     2221 gcgcgggatg acgcccatgc ggttggagaa gacctccggt tggcgcagga cctcgcgcac
     2281 gaggtcgtag gtgccgactt gcaggaagga aaagccgttg gctgcctcga cgtggacgac
     2341 cgggcaccgc tcccgatcgc ggcggatctg gtgccacgga tcggggtcgg tcgatttgaa
     2401 ggtatcgaac cccaggcggt gctccgaggc cacggctcag gcccgcccga aagcgcgggc
     2461 cacggcgcgg aaggcgtcga ggttctcgac gatgggctcg tggctgagct ggtcgccgta
     2521 gcgaacgatc atcccggaga gcccgcaatc gcgccaccgg cgggcgcgtt cctcgatccg
     2581 ggcgacgggg cccaccagcc agccgttgtc gatgtattcg tcgggcacgg cagcgatggc
     2641 ctcttcccac agtggatcgc gcaggaattt ctccccggcc tggagccgcg cttcgggatc
     2701 ggcgcccggg tcctgcgcga ccagttcggc gaggcggtcg gccacgtcct tgaagccgcg
     2761 ggcgatcatg aactcgcggt ggatggctga ccaggtaacc acgtattcct tgaagggccg
     2821 catcgcggcc cggacgtcgt catcgaccag catgtcgacg tgggcccaga tcttgtagtc
     2881 cttcggctcg ccgccggggc gcctggcaaa gccctggtcc agcagcgggc ggaacatctg
     2941 gtaatcgccg atatgccagc ccggcggcat ccacccgtcg ccgatctcgg cggcgagggc
     3001 gatcatctgc gggcccgagg cggcgatgca caccgggatc tcgcccatcg gggcgaggcc
     3061 cagcggcgcc gggggaaggc catgcgcgcc cgggccgcgg tagggagcgc accattcctg
     3121 gccgtcgtga tccagcggct ggccctcgag accctggcgc aggatcgtca cgaaatcccg
     3181 cattcgccgc accggcttgc cccacggcag cccctggaac ccctcgcgcg cctggagagc
     3241 ggcggtgccg acgccggcga tgatccggtt gccgccggtg agatggttga gcgtctgcaa
     3301 cgacatggcg gttacggcgg gcgggcgccc gggca
//
LOCUS       DATNBV010000105         3444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1333904,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000105 DATNBV010000000
VERSION     DATNBV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333904"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /gene="priA"
                     /locus_tag="VN627_02555"
     CDS             complement(<1..1473)
                     /gene="priA"
                     /locus_tag="VN627_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXD02166.1"
                     /translation="MPLPLAGSYDYLGEGLAPGDFVVVPLGKRETIGVVWGPGTGEVP
                     ESKLKAVIAKLDAPPLPEITRRFIDRVAAYTLAPPGAVLRMAMSVSTALEPPRMIAAW
                     RLAPEHAPAPGQAAAPGQATSKDTPARRRVRAVLQQGPARSTSDLAREAGVGTGVVKA
                     MAAAGLLEAVSLPPLPAFRRPDGFHEGPALSPAQRAAAESLIAHLADGSVTLLDGVTG
                     SGKTEVYFEAVAATLRAGRQALVLLPEIALTGQWLERFEQRFGARPAEWHSDLTGAER
                     RVAWRAIAMGEARVVVGARSALFLPFPDLGLVIVDEEHEQAYKQEDGVAYQARDMAVL
                     RGHLARIPVVLVSATPSLETLKNVAGGRYRALHLPDRHAGAALPKVRAVDLRRDRPAR
                     QRWLSPGLVSALEETLAQGEQALLFLNRRGYAPLTLCRHCGHRMNCPNCTAWLVEHRH
                     AGRLQCHHCGYQARLPEACPSCGTAGSFAACGPGVERLAEE"
     gene            1541..2197
                     /gene="fsa"
                     /locus_tag="VN627_02560"
     CDS             1541..2197
                     /gene="fsa"
                     /locus_tag="VN627_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HXD02167.1"
                     /translation="MKFFVDTADIAEIRDLAATGLVDGVTTNPSLVAKSGRRFLDVVK
                     EICDVVPGPVSAEVAALDHAGMLGEGRKLAKIAHNVVVKVPLTPEGLKTCKALRAEGT
                     KVNVTLCFSPAQAILAAKAGASYVSPFVGRLDDVGVDGMGLIGDIVQIYDSYPEFETE
                     VLVASVRHPMHVVAAGKLGADVVTLPPPVLRQMFNHPLTDKGLAAFLADWAKTGQSIV
                     "
     gene            2215..2928
                     /locus_tag="VN627_02565"
     CDS             2215..2928
                     /locus_tag="VN627_02565"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HXD02168.1"
                     /translation="MAQNPDDAIAASPRITAAQVVDYLKRHPNFLQNHPELLDSQTPP
                     ARAQGEGVVDLQQFMVDRLRRDLTRLRGLQDELVANSRDNLSTQDRIHKAILALLDAE
                     TFEHLIEIVTTDLAVLLSVDVAALCVEAATGSAPRSVEGVQVLPSGFVDGLLGAGHEV
                     LLRDDIEGDETIFGPAAGLVRSDALIRLTVGRSAPPALLVFGTRHPGYFNAGQGTELL
                     SFLARVIEHCLRLWLGLKA"
     gene            2907..>3444
                     /locus_tag="VN627_02570"
     CDS             2907..>3444
                     /locus_tag="VN627_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXD02169.1"
                     /translation="MAGAEGLKPSPAAGDLAEPVDAWRNWLKSERRAASLSLEAYERD
                     LFAFLAFLAEHQGAPPSLQSLRDLSRGDLRAWLAARGGRGLKATSTARALSVIRGFFR
                     FLARRGLVENAAVLALRNPKLPKSVPRALSEAEAMDALDNIGELAGEPWIAKRDTALL
                     TLLYGCGLRLGEALSLTRA"
BASE COUNT          528 a         1224 c         1168 g          524 t
ORIGIN      
        1 ctcctctgcc agccgctcga ccccgggccc gcaggccgcg aagctgccgg ccgtgccgca
       61 ggaagggcag gcctcgggca gccgcgcctg ataaccgcaa tggtggcatt gcagccggcc
      121 ggcatggcga tgctccacca gccaggcggt gcagttgggg cagttcatgc gatggccgca
      181 atggcggcag agggtcagcg gcgcataacc ccggcgattg aggaacagca gcgcctgctc
      241 gccctgggcc agcgtctcct ccagcgccga gaccaggccg ggggagagcc agcgctggcg
      301 cgccggccga tcgcgccgga gatcgacggc gcggaccttg ggcaatgccg ccccggcatg
      361 gcggtcgggc agatgcaggg cccgatagcg cccgccggcg acattcttca gcgtctccag
      421 ggacggcgtg gcggagacca ggaccacggg gatgcgggcg agatggcccc gcagcacggc
      481 catgtcgcgg gcctgatagg cgacgccatc ctcctgctta taggcctgct catgctcctc
      541 atcgacgatg acgaggccga gatcgggaaa gggcaggaac agcgccgagc gcgcgccgac
      601 cacgacccgg gcctcgccca tcgcgatcgc ccgccaggcg acgcggcgct ccgcaccggt
      661 gagatcggaa tgccattcgg ccggccgggc gccgaagcgc tgctcgaagc gctcgagcca
      721 ctggcccgtc agcgcgatct cgggcagcag caccagcgcc tggcgccccg cccggagcgt
      781 tgcggccacg gcctcgaaat agacctcggt cttgcccgag ccggtgacgc cgtccagcaa
      841 ggtgacgctg ccatcggcca gatgggcgat caggctttcg gccgccgcgc gctgtgccgg
      901 cgagagcgcc gggccttcat ggaatccatc gggccggcga aaggcgggaa gcggcggcag
      961 gctgacggct tcgagcaggc ccgccgccgc catcgccttg accacgccgg ttcccacccc
     1021 ggcctcgcgc gcgagatcgc tcgtgctgcg cgccgggccc tgctgcaaga ccgcgcgcac
     1081 gcgccggcgc gcaggcgtgt ctttcgacgt ggcttggccc ggcgctgccg cttggcctgg
     1141 cgcaggcgcg tgctccggcg ccaagcgcca ggcggcaatc atccgtggcg gctcgagcgc
     1201 cgtcgacacg ctcattgcca tgcgcagcac ggcgcccggc ggcgccaatg tgtaagccgc
     1261 gactcgatcg atgaaacgcc gcgtgatctc gggcaacggc ggcgcgtcga gcttggcgat
     1321 gaccgccttc agcttgctct ccgggacctc gcccgtgccc ggcccccaga ccacgccgat
     1381 ggtctcgcgc ttgcccaaag gcacgacgac gaaatcgccc ggcgcgaggc cttcgccgag
     1441 gtagtcgtag ctgccggcca gcggcaaagg caacaagatg gcaagggagt ctggcatcgg
     1501 ctacacttta acgcgtcgag actgattcgc cttggtgagc atgaagttct tcgtggatac
     1561 cgccgatatc gccgagattc gcgacctcgc cgccaccggg ctggtggacg gcgtgaccac
     1621 caacccgtcg ctggtggcca agagcgggcg cagattcctc gatgtcgtca aggagatctg
     1681 cgatgtggtt cctggacccg tcagcgccga ggtggcggcc ctcgatcatg ccggcatgct
     1741 gggcgaggga cgcaagctcg ccaagatcgc gcataatgtt gtcgtcaagg tgccgctgac
     1801 gcccgagggg ttgaagacct gcaaggcgct gcgcgccgaa ggaaccaagg tcaatgtcac
     1861 gctctgcttc tcgccggccc aggccattct cgccgccaag gcgggcgcca gctatgtctc
     1921 gcccttcgtc ggccggctcg acgatgtcgg cgtcgacggc atgggcctga tcggcgacat
     1981 cgtgcagatc tacgactcat atcccgaatt cgagaccgag gtgctggtcg cctcggtgcg
     2041 ccaccccatg catgtggtgg ccgcgggcaa gctcggcgcc gatgtcgtca ctttgccgcc
     2101 gccggtgctg cgacagatgt tcaatcaccc cctcaccgac aagggtctgg ccgcgttcct
     2161 cgccgattgg gcgaagaccg gccagtcaat cgtttaaaat ggcgatcctc tagcttggcc
     2221 cagaatcccg acgacgcgat cgcagcatcc ccgcgcatca ccgccgcgca ggtggtcgat
     2281 tatctgaaac gtcatcccaa tttcctccag aaccatccgg agcttctgga cagccagacg
     2341 ccgcccgccc gcgcgcaggg cgaaggcgtg gtcgatctgc agcagttcat ggtcgaccgg
     2401 ctgcgccgcg atctgacgcg gctgcgcggc ctgcaggacg agctggtcgc caatagccgc
     2461 gacaatctct ccacccagga ccgcatccac aaggcgatcc tggcgctgct cgatgccgag
     2521 accttcgagc atctgatcga gatcgtcacc accgacctcg ccgtgctgct gagcgtcgat
     2581 gtggccgcgc tttgcgtcga ggccgccacc ggcagcgcgc cgcgctcggt cgagggcgtg
     2641 caggtcctgc ccagcggctt cgtcgacggg ctgctggggg ccgggcatga ggtgctgctg
     2701 cgcgacgata tcgagggcga tgagaccatc ttcggcccgg ccgccgggct ggtgcgttcc
     2761 gatgccttga tccgcctcac cgtcggccgc agcgcgccgc ccgccctgct ggtcttcggc
     2821 acccgccatc ccggctattt caatgccggc cagggcaccg agctgctgag tttcctcgcc
     2881 cgcgtcatcg agcattgcct ccggctatgg ctggggctga aggcctgaaa ccttcgcccg
     2941 ccgccggcga tctcgccgag ccggtggatg cctggcgcaa ctggctgaaa tccgaacgac
     3001 gcgcagcatc cctcagcctc gaggcctatg agcgcgacct cttcgccttc ctcgccttcc
     3061 tggcggaaca tcagggcgcg ccgccctcgc tgcagagcct gagggatctc agccgcggcg
     3121 atctgcgcgc ctggctggct gcccgcggcg gccgcggcct caaggcgaca tcgacggcgc
     3181 gcgccctcag cgtgatccgg gggttcttcc gcttcctcgc gcgccgcggg ctggtggaga
     3241 acgccgccgt gctggcgctg cgcaatccca agcttccgaa atcggtgccg cgggcgctca
     3301 gcgaagccga ggcgatggat gccctcgaca atatcggcga gctggcgggc gagccctgga
     3361 tcgccaagcg cgacacggcg ctgctcacgc tgctctatgg ctgcggcctg cggctgggcg
     3421 aagcgctctc gctgacgcgc gccg
//
LOCUS       DATNBV010000106         6163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1975635,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000106 DATNBV010000000
VERSION     DATNBV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1975635"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..1130)
                     /locus_tag="VN627_02575"
     CDS             complement(387..1130)
                     /locus_tag="VN627_02575"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXD02170.1"
                     /translation="MVAFAFVSKFRALAGLAAGLASLITANPALANASANADILAPLR
                     AAQAAKHQSGDEEFRQLFSSWQSMDKPAARQNLVSIARPASSIGGAIPGFAPQKVSIP
                     SLVPVDGVKLTSDYGMRWHPVLGGRRQHKGVDLAAPIGTPIHAAADGMVERADWFSGY
                     GLYVAIEHGGEIETRYGHMSRLNVAAGQMVHKGDIIGYVGTTGRSTGPHLHYEVRVAG
                     EAVNPMPYLQSGNIQRATGTNLAMGAGEE"
     gene            complement(1280..2089)
                     /locus_tag="VN627_02580"
     CDS             complement(1280..2089)
                     /locus_tag="VN627_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXD02171.1"
                     /translation="MGPSVAAAIRAALLTPDPRAKAMATRQVARDWRLGRLAFAFDAA
                     MPDRPARPPTPELLPPNRMPKRGRGNSERGRIALWHALAHIEFVAIDLALDMAGRFGA
                     DMGEVFVGDFLKVAADEAMHFALLERHLATLGSHYGALPAHDGLWESAHETRHDVAAR
                     LAVVPMVLEARGLDVTPATLERVRATGDWRGARILERILDDEIRHVRVGASHFESCAR
                     LRQINAAPLWKSLVVRHFRGAIKPPFNDSARAAAGLSRDLYGEVATAERAG"
     gene            complement(2104..2574)
                     /locus_tag="VN627_02585"
     CDS             complement(2104..2574)
                     /locus_tag="VN627_02585"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXD02172.1"
                     /translation="MTQPTVGASLPDIALALSDGTAVRPADYAGRKLVLFFYPKDDTP
                     GCTTENLDFTALAAEFEQAGTALLGISKDSAERHKKFAAKHGLTVPLATDAAEGGLSD
                     ALGIWTEKVNYGRTYMGMVRTTLLVDAQGKIARVWSKVKVKGHAAEVLEAAKAL"
     gene            complement(2576..5620)
                     /locus_tag="VN627_02590"
     CDS             complement(2576..5620)
                     /locus_tag="VN627_02590"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HXD02173.1"
                     /translation="MREADPDPVLRVPFGRHGQGIAHGAAPCQSRCLPASAVAGPGSR
                     RERRERSGAKRPCCAAALIISRFPIPGRLHRAELSASSAPSARTLDRRHLTGHRRSEE
                     TTVPETPANSADWTNALTRARDHAPFLAGLIERQGDLASLLAAGDGEAALTHARAAGT
                     HAPSVGAALRRERRALALALAVGDLAGAFPLARVMAELSAFADRALDAAIAEAVVERV
                     PDAAPAGFLALALGKHGAQELNYSSDIDPILLFDPAKLPRRERDDPGEAAARVARRVV
                     QLLSHVDEEGYVFRVDLRLRPASEVSPPALSIDAALTHYESSALAWERAAFIRARACA
                     GDLPAGESFLAAIRPFVWRKSLDFGAIAEIGRLTARIRQAKEGPARPGPGFNLKTGRG
                     GIREVEFYAQTHQLIHGGRHPALRQRGTRAALDALAAAGIIDPADAQVLGESYDRLRT
                     LEHRLQMVLDQQTHALPADPAALDNVARLDGLADGAALVAELEQVTEAVGTRYDALIA
                     AHGDVGNTAVAVPDQGQPRPLAAELAELGFAEPRQLAERIDGWRGGTVRALRSDAARL
                     ALDAIQPALLAALARSPEPSRAFTRWEELLARLPSALNLFHLLENRPALLELLAKVLG
                     LAPPLADALARRADLLDPLIDATAFDLPPDTATLAAELADGEEGDDYQRLLDRVRRKV
                     SERRFTLGVQLIEGASDPLAIAAANSRLAEAALRVLAEATIREFAATHGRVPGGELVI
                     LGLGRLGGGALTHASDLDLVYLFTGAHETESDGSRPLGATLYFNRLAQRISAALSVPT
                     AEGALYEVDTRLRPSGTQGPLAVSFDSFERYQREQAWTWEHMALCRARPLFGPAEARS
                     ELARIIREVLSAPRDAAKLKADVLDMRARMAEHKPGRGALDAKLRRGGLVDVEFLVHY
                     RQLATAQGLTPDLGQAIDQLATAGLVPADLRLAHDRLTRLLVAARLLAPDGEEPPPGP
                     RAALAAATGYANWDSVTAGFAAGRQIVARAWAQVFGETLELE"
     gene            5552..>6163
                     /locus_tag="VN627_02595"
     CDS             5552..>6163
                     /locus_tag="VN627_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HXD02174.1"
                     /translation="MSNALTMPPEWHPQDWIWIGFPHDAEEWPGFLGQAQEQIAAFAS
                     AVAESGQAVKLVVRDAANRARAESLVGPGVELVERVYGDVWLRDTGPLVLCGPDGSRA
                     ARRFGFNGWGEKYLMDGDQTIGADLARDAGLEITMSDWILEGGALDTDGTGLVVTTEQ
                     CLLNPNRNPHLPRGEIEARLAVDLGFTRVLWLGEGLINDHTDGH"
BASE COUNT          970 a         1846 c         2423 g          924 t
ORIGIN      
        1 gacgaatcgg agctgccgtg gaaggtcgat ctggttgacc ggcgcgcagt cagccaagcg
       61 ttcggacaga tcatcgatgc cggacgtgta gcgctcaatt gacgcctcaa aagaaaggcg
      121 attgcgtcgt ttgcaaacac gacagctttg gaaaccctcc ccgctgcgac taaggcgcac
      181 ctgcggtgcg ccaagtctcg ctcccctccc tcaagggagg gggtgggttt acgacgcttg
      241 cgtttacaaa ggacataagt ctcaaaagaa aagggcggcc tgagccgccc ccttccgatg
      301 caccctctcc gagtacgtct accggctgca tcttgccgtg cagtccggtt gggctgaccc
      361 tcagagcccc ctgatatgcg aaactcttat tcttcgcccg cacccatcgc caggttggtg
      421 cccgtggcgc gctggatgtt gcccgactgg aggtagggca tcgggttcac ggcttcgccg
      481 gcgacgcgca cttcatagtg gaggtgcgga ccggtcgagc ggccggtggt gccgacatag
      541 ccgatgatat cgcccttgtg gaccatctgg cctgcggcga cattgaggcg cgacatgtgg
      601 ccgtagcggg tttcgatttc gccgccgtgt tcgatggcga catagagacc atagcccgag
      661 aaccaatcgg cgcgttcgac catgccatcg gcggcggcgt ggatcggggt gccgatcggg
      721 gcggcgagat cgacgccctt gtgctggcgg cgaccgccga ggaccgggtg ccagcgcatg
      781 ccataatcgc tggtgagctt cacgccatcg accggaacga gcgaggggat cgacaccttc
      841 tgcggggcga agccggggat cgcgccgccg atggacgaag cggggcgggc gatggaaacg
      901 aggttctggc gggcggcggg cttgtccatc gactgccagg acgagaacag ctggcggaat
      961 tcctcatcac ccgactggtg cttggcggcc tgggcggcgc ggagcggggc gaggatatcg
     1021 gcgttggcgc tggcgttggc gagagctgga ttggcggtga tgagcgaagc gagcccggcg
     1081 gcgaggccag ccagagcacg aaacttcgac acgaaagcaa aagcgaccac tcagcagtac
     1141 cttgtcttgc ggcatgcctg ccgggggcag gcgggccgtg cgacccctgt cttatccggc
     1201 aaccaaagcc ctggtcggcc ctgtgaaacc gttcgattct gagatggtcg gaaccccccg
     1261 ccgtctcggt ggttgggagc taaccggccc tttcggccgt ggcaacctca ccatagagat
     1321 cgcgcgataa accggctgcc gcccgcgccg agtcgttgaa cggtggctta atggcccccc
     1381 ggaagtggcg gacaaccaag cttttccaca agggcgccgc attgatctgg cgcaacctgg
     1441 cgcaggattc gaaatggctt gcgcccacgc ggacgtgacg gatttcgtcg tcaaggattc
     1501 gttcgaggat gcgggcgcct cgccaatcac cggtagcgcg aacccgctcg agcgtggctg
     1561 gagtgacatc gaggccgcgg gcctcgagca ccatggggac gacggcgagg cgggcggcga
     1621 catcgtggcg ggtttcgtgg gccgactccc acaggccgtc atgcgccggg agggcgccgt
     1681 agtggctgcc cagcgtggcg aggtggcgtt cgagcagggc gaagtgcatg gcctcgtcgg
     1741 cggcgacctt gaggaaatcg ccgacgaaca cttcgcccat gtccgcgccg aagcggccag
     1801 ccatatcgag cgcgaggtcg atggcgacga attcgatatg ggcgagggca tgccacaggg
     1861 cgatgcggcc ccgttcggaa ttgccgcggc cgcgcttggg catgcggttt ggcgggagca
     1921 gttcgggcgt gggcggacgg gccgggcgat cgggcatggc ggcgtcgaac gcgaaggcga
     1981 gccgcccgag ccgccaatct cgcgccacct gccgcgtggc catggccttg gcgcgcggat
     2041 cgggcgtgag cagggccgca cggatggccg cggcaacgga agggccaatc acgggggctt
     2101 cgctcaaagc gctttcgcgg cctccagcac ttcggcggcg tggcccttga ccttcacctt
     2161 cgaccagacg cgggcgattt ttccttgtgc atcaaccagc agggtcgtgc ggaccatgcc
     2221 catgtaggtg cggccgtagt tgaccttctc ggtccagatg ccgagcgcat cggacaggcc
     2281 cccttcggcg gcatccgtcg caagggggac ggtgaggccg tgcttggcgg cgaacttctt
     2341 gtggcgttcg gcactgtcct tggagatgcc gaggagggcg gttccggcct gctcgaattc
     2401 agccgcgagt gcggtgaaat caaggttttc ggtggtgcag ccgggggtat cgtccttggg
     2461 gtagaagaac aggacgagct tgcgcccggc atagtcagcc gggcggacgg cggtgccgtc
     2521 agacagggcg agggcaatat cggggaggct tgcgcccacg gtcggttggg tcatctcact
     2581 cgagctccag ggtttcgccg aagacttgcg cccaggcacg ggcgacgatc tgccggccag
     2641 ccgcgaatcc ggcggtcacg ctgtcccaat tggcatatcc tgtggccgcc gcaagggccg
     2701 cgcgcgggcc ggggggcggc tcctcgccat cgggggcgag caggcgggcg gcgacgagca
     2761 ggcgggtgag ccggtcatgg gcgaggcgca ggtcggcggg gacgaggccg gccgtggcaa
     2821 gctggtcgat ggcttggccg agatcggggg tgaggccctg ggccgtggcc agctggcggt
     2881 aatggacgag gaattcgaca tcgacgagcc cgccgcgcct gagcttggca tcgagcgcgc
     2941 ccctgcccgg cttgtgctcg gccatgcggg cgcgcatgtc gaggacgtcg gccttgagct
     3001 tggcggcatc gcgcggggcg gagaggactt cgcggatgat ccgggcgagt tcggaccggg
     3061 cttctgcggg gccgaacagt gggcgggcgc ggcagagggc catgtgttcc caggtccacg
     3121 cctgttcgcg ctgatagcgt tcgaacgaat cgaagctgac agcgaggggc ccctgggtgc
     3181 cggacgggcg caggcgggtg tcgacctcat agagcgcgcc ttccgccgtg gggacggaga
     3241 gggcggcgct gatccgctgg gcgaggcggt tgaaatagag cgtggcgccc aagggacggg
     3301 agccgtcgga ttcggtctcg tgggcgccgg tgaacaggta gacgaggtca aggtccgagg
     3361 catgggtgag cgcgccgccg ccgagccggc cgaggccaag gatgacgagc tcgccgccgg
     3421 gaacgcggcc gtgagtggcg gcgaattcgc ggatcgtcgc ctcggcaagg acccggagcg
     3481 cggcttcggc gaggcgggaa ttggcggcgg cgatggccag cggatcggaa gcgccctcga
     3541 tcaactggac gccgagggtg aagcggcgtt cggacacctt gcggcggacc cggtcgagca
     3601 ggcgctggta atcgtcgcct tcctcgccat ccgcgagttc ggcggcgagg gtggcggtgt
     3661 cgggcgggag gtcgaaggcg gtggcgtcga tcagcggatc gagcagatcg gcccggcgag
     3721 cgagggcatc ggccagcggc ggggccaggc cgaggacctt ggccagcagc tcgagcagcg
     3781 ccgggcggtt ttcgagcagg tggaacaggt tgagcgccga gggcaggcgg gcgagcagtt
     3841 cttcccagcg ggtgaaggcc cggctcggct ctggcgaacg cgcgagggcg gcgagcagcg
     3901 cgggctggat cgcgtcaagc gccaggcggg cggcatcgga gcgcagggcg cgcaccgtgc
     3961 cgccgcgcca gccgtcgatc cgttccgcca gctggcgcgg ttcggcaaag ccgagttccg
     4021 ccagttcggc ggcgagcggg cggggctgac cctgatcggg cacggcaacg gcggtgttgc
     4081 cgacgtcgcc atgggcggcg atcagggcat cgtagcgcgt gccgaccgcc tcggtgacct
     4141 gctcgagctc ggccaccagc gcggcgccgt cggccaggcc gtcaagccgg gcgacattgt
     4201 cgagcgcggc gggatcggcc gggagcgcgt gggtctgctg gtcgagcacc atctgcaggc
     4261 ggtgttcgag ggtgcgcagg cggtcgtagc tttcgcccag cacttgtgca tcggcggggt
     4321 cgatgatgcc ggcggcggcg agggcatcga gcgcggcgcg ggtgccgcgc tggcgcaggg
     4381 cggggtggcg gccgccgtgg atcagctggt gggtctgggc gtagaactcg acctcgcgaa
     4441 tgccgccgcg tccggtcttg aggttgaagc cggggcccgg gcgggcaggt ccctccttgg
     4501 cctggcggat gcgcgcggtg aggcggccga tctcggcgat ggctccgaaa tcgaggctct
     4561 tgcgccacac gaaggggcgg attgccgcta gaaaggattc accggcgggc aggtcgccgg
     4621 cacaggcccg ggcgcggatg aaggcggcac gctcccaggc aagggccgag ctttcgtagt
     4681 gggtgagcgc ggcgtcgatg gaaagagccg gcggggagac ttcggaggcg gggcgcaggc
     4741 gcaggtcgac gcggaagaca tagccctcct cgtcgacatg ggagagcagc tggaccacgc
     4801 ggcgggcgac gcgggcggcg gcctcccccg gatcgtcgcg ctcgcgccgg ggaagcttgg
     4861 cgggatcgaa caggagaatg ggatcgatgt cggacgagta gttgagctcc tgcgcgccgt
     4921 gcttgcccag ggcgagggca aggaaaccgg caggtgcggc atcggggacg cgttcgacga
     4981 cggcctcggc gatggcggca tcgagcgcgc ggtcggcgaa ggcggagagt tcggccatta
     5041 cccgggccag cgggaaggcc ccggcgagat cgcccacggc aagggcaagg gcaagggcgc
     5101 ggcgttcgcg gcgcaaggcg gcgccgacgg agggggcatg ggtgccggcg gcgcgggcgt
     5161 gggtgagggc ggcttcgccg tcgccggcgg cgaggaggct ggcgaggtcg ccctggcgtt
     5221 cgatcagccc ggcgaggaag ggggcatgat cgcgagcgcg ggtgagggca ttggtccagt
     5281 ccgctgaatt ggccggggtt tcaggcactg tcgtctcctc ggagcggcga tgcccggtta
     5341 ggtggcggcg atcaagggtt cgcgcagagg gcgcagagga cgcagagagt tctgcacggt
     5401 gaagccgccc gggaatcggg aaccgggaga tgatcaaggc tgctgcgcag cagggtctct
     5461 ttgctccgct gcgttctctg cgctctctgc gcgaacccgg accagccaca gcgcttgccg
     5521 gaaggcacct ggactggcag ggggccgcgc catgagcaat gccttgacca tgccgcccga
     5581 atggcacccg caggactgga tctggatcgg cttcccgcac gatgccgagg aatggccggg
     5641 gttcctgggc caggcgcagg agcagatcgc cgcctttgcg agcgcggtcg ccgaatccgg
     5701 gcaggcggtt aagctggtgg tgcgcgatgc ggccaaccgg gcgcgggcgg agagcctcgt
     5761 cggcccgggg gtcgaactgg tcgagcgggt ctatggcgat gtgtggctgc gcgataccgg
     5821 gccactggtg ctctgcgggc cggacggcag ccgcgcggcg cggcggttcg gcttcaacgg
     5881 ctggggcgag aaatacctga tggacgggga ccagaccatc ggcgcggacc tggcccgcga
     5941 tgccgggctg gaaatcacga tgtccgattg gatcctcgag ggcggggcgc tggatacgga
     6001 cggcaccggg ctggtggtga cgacggagca gtgcctgctc aacccgaacc gcaatccgca
     6061 cctgccgcgc ggcgagatcg aggcgcggct ggcggtggac cttggattta cccgcgtgct
     6121 gtggctgggc gaggggctga tcaacgacca taccgacggg cat
//
LOCUS       DATNBV010000107         4780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_80537,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000107 DATNBV010000000
VERSION     DATNBV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80537"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VN627_02600"
     CDS             <1..519
                     /locus_tag="VN627_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HXD02175.1"
                     /translation="LKAVGADFVYLDASASAFARDPAFVKNLEDARAAKLMVGAVHLY
                     DPCQPADKQAANFVTVIPRDAKMLPPAVDLDRLADDCPMPVSDAAVESELTTFLNQIE
                     NHTGKAAILKLSRRFERRYHIAAAISRNLWLTGERIQPDYAGRPWMLWTANTRLVTDA
                     SPGPLRWVVARP"
     gene            542..988
                     /locus_tag="VN627_02605"
     CDS             542..988
                     /locus_tag="VN627_02605"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HXD02176.1"
                     /translation="MSTPPTRAALIAAAQTASARAHAPYSGFHVGAALGFADGTLVTG
                     ANVENASYGLSLCAETVAVAAAMNAGARGGLVAVAVLGGPPGHDGEAMPGPDPVTPCG
                     RCRQVLAELAGLGATDPVVWCVSNEEVLETRLSELLPHAFRAGQLG"
     gene            1109..2035
                     /locus_tag="VN627_02610"
     CDS             1109..2035
                     /locus_tag="VN627_02610"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXD02177.1"
                     /translation="MNRPAMLQSKPLVEGLSFAEGPRWHDGWLWFSDIARDRVMRVRE
                     DGSDLQTVAAIESPSGLGFLPDGTLLVVGMKSRQLHRVSREGGVSLHADLSAHGAILN
                     DMVVTAEGRAYVDCYRPGPAVPPPAGPDGSPCSLSGDIDRYYLNGLGFSHNLAGEILV
                     VHPDGTSAVAASGLNYPNGLGITADGRTLIASISHESRLTAFDIGENGTLANPRLWAD
                     LPGRHPDGLCLDADGGVWVACLAISAFLRVTEGGFITHHIPTYGRWSIAPALGGADRR
                     TLFMISMEPIDVPGHRSWIEHVRVSSSGAGLP"
     gene            complement(2078..3118)
                     /locus_tag="VN627_02615"
     CDS             complement(2078..3118)
                     /locus_tag="VN627_02615"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXD02178.1"
                     /translation="MPETIRPATGKAVWTAADLGDPAGLGSPFSKDEIAEMERVTDQA
                     RQRGITAETAPDNLFDWPLVDRRMKQVLAELRDGSGLALLTGLPTDRFGKDDLQLIFW
                     GLGRSIGAAVPQSAKGDRLGQIRDMTHIDPNARAYQNRNELNPHTDHTEVVGLMCLRD
                     AMEGGFTPLTNALAVHNEILRQRPDLMERLYRGYRYHRRGEEQDGQEPITPHRVPVFS
                     RLGEAVSCRLIRPYIEAAAAELGEPLSAADLTALDLVQQTARDPRFRLGLRLEPGSLV
                     LFNNYTMFHERTPFTDWPEEDRKRHLVRMWLVPDDLRPVVPEIELYGTKGGAILRSGG
                     PARSFGWGVGGQ"
     gene            3405..4433
                     /locus_tag="VN627_02620"
     CDS             3405..4433
                     /locus_tag="VN627_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02179.1"
                     /translation="MIRSKTTFVLGPGAAAELHFPGAAETLERIAAALDFSRATANQM
                     TRDTAAILRHVGKLAERTGRSEEQLYRAAQRIHQSAKVGRTVEGVIDQNDDDPLVEAF
                     GKLALAVFTLQSEQRSSVRAAPQVAGGLPMQTGDYWLTELGRLICASLPRSRLEQGLA
                     DVSLVSFTYDRSAEHVLPYVVSTAYGMTLQEAQRMVASRLKVFHPLGSVGRLPWQSGD
                     GPDVDWGNEQAWNIAALSAHIRTAGQAMKDRVALGALRAAVAASSRIVFLGFDFAPAA
                     LDLMVDGSLSHNPELVICAPGLVGPNRDAAIRMLKRKTGVERDDRLIFAEGRCLDLLK
                     DYTLLLES"
     gene            complement(4486..>4780)
                     /locus_tag="VN627_02625"
     CDS             complement(4486..>4780)
                     /locus_tag="VN627_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02180.1"
                     /translation="QGYARYRRILERRTPDFQTRLGYPVARPGAANLAMSTNQLAERF
                     GRSHGCVSMTLEMPFKDNDDLPDPMHGWSPARSRQLARDCLAALGEWLETGAA"
BASE COUNT          728 a         1687 c         1553 g          812 t
ORIGIN      
        1 ctcaaggctg tcggcgcaga tttcgtctat ctcgatgcct cggccagcgc cttcgcgcgc
       61 gatccggcct tcgtgaagaa cctcgaggat gcccgcgccg ccaagctgat ggtcggcgcg
      121 gttcacctct acgatccgtg ccagccggcc gacaagcagg ccgccaattt cgtcaccgtc
      181 atcccgcgcg acgcgaagat gctgcccccc gctgtcgatc ttgaccggtt ggccgacgat
      241 tgccccatgc cggtcagcga cgccgcggtg gaaagcgagc tgaccacttt ccttaaccag
      301 atcgagaacc acaccggcaa ggccgcgatc ctcaagctct cgcgccggtt cgagcggcgc
      361 taccacatcg ccgcggcgat ctcgcgcaac ctgtggctga cgggcgagcg catccagccc
      421 gactatgccg ggcgtccgtg gatgctgtgg accgccaaca cccgccttgt caccgacgcc
      481 agccccggcc ccttgcgctg ggtagtggcg cgcccttgaa gatgggcgca taaccggcgc
      541 catgtccact cctcccaccc gcgctgccct gatcgccgcc gcccagaccg cctcggcccg
      601 cgcccacgcg ccctattcgg gcttccatgt cggcgccgcg ctcggctttg ccgatggcac
      661 gctggtcacc ggcgccaatg tcgaaaacgc cagctacggc ctctcgctct gcgcggaaac
      721 cgtggccgtg gccgcagcca tgaacgcggg cgcacgcggt ggcctcgtcg ccgttgccgt
      781 cctcggtggc cccccgggtc acgacggcga agcgatgccc ggacccgatc ccgttacccc
      841 ctgcggccgc tgccgccagg tcctggccga actggccggg ctgggggcga ccgacccggt
      901 ggtctggtgc gtttcgaacg aggaagtgct cgaaacgcgg ctgtcggaac tgctgcccca
      961 tgccttccgg gcggggcaat tgggctgagc agtctcccac ccgtcgcacg gatgtggcga
     1021 cagcggccat gccgttgcct ccaacccatt tatctgatgc aactggcaaa accggccggc
     1081 gctgcgccgg cggagggaag ggtgatccat gaacagaccg gccatgctcc agtcgaaacc
     1141 gctggtggag gggctcagct tcgccgaagg tccgcgctgg catgatgggt ggctgtggtt
     1201 ctccgacatc gcccgcgacc gggtcatgcg ggttcgcgag gatggcagcg acttgcagac
     1261 cgtcgccgcg atcgagagcc cttcggggct gggcttcctg ccggatggaa cactgctcgt
     1321 cgtcggcatg aaatcccgcc agcttcaccg cgtaagccgg gagggagggg tgagcctcca
     1381 cgccgatctg tcagcccatg gcgccatctt gaacgacatg gtggtcacgg cagaaggccg
     1441 ggcctacgtc gactgctacc ggcccggccc cgccgtcccc ccgccagccg gaccggatgg
     1501 ctctccctgt tcgctctcgg gcgacatcga tcgctactat ctcaacggcc tcggcttttc
     1561 ccacaacctc gcgggagaga tcctggtggt tcacccggac ggtacgagtg ccgttgccgc
     1621 gtcaggcctc aactatccca atggcctggg catcaccgcc gatggacgca ctttgatcgc
     1681 gagcatttcc cacgaaagcc ggctgacggc cttcgacatc ggtgagaacg gcaccctcgc
     1741 caatccccgc ctgtgggccg acctgcccgg ccgccatccc gatggcctgt gcctcgatgc
     1801 cgatggcggc gtgtgggtcg cctgcctcgc gatcagcgcc ttcctgcggg tcaccgaggg
     1861 cggcttcatc acccatcata tccccaccta cgggcgctgg tcgatcgcgc cggcgctggg
     1921 aggcgcggac cggcgcacgc tgttcatgat cagcatggag ccgatcgacg tgcccggtca
     1981 tcgttcgtgg atcgagcatg tccgcgtgtc ttcctccggt gccgggctgc cctgaccggg
     2041 caatcctgca tcccggggcg gggcggtacc gcgtccgtca ttgtccaccc accccccagc
     2101 cgaatgatcg ggccgggccg ccgctgcgca ggatcgcccc gcccttggtc ccgtaaagct
     2161 cgatctcggg gacaaccggc cgcagatcgt cgggcaccaa ccacatccgc accaggtggc
     2221 gcttgcgatc ctcttcgggc cagtcggtga atggcgtgcg ctcgtggaac atggtgtagt
     2281 tattgaacaa taccaggctg cctggctcca gccgcaggcc aaggcggaac cgcgggtccc
     2341 gcgcggtctg ctggaccagg tccagcgcgg tcaaatccgc ggcgctcagg ggctcgccga
     2401 gttccgccgc ggcggcttcg atgtaggggc ggatcagccg gcagctgaca gcctccccca
     2461 ggcgggagaa aaccggcacc cggtgcggcg tgattggctc ctggccatcc tgctcttcgc
     2521 cccggcggtg atagcggtag ccacgataga gccgctccat gaggtcaggg cgctggcgca
     2581 ggatctcgtt atgcactgcc agtgcattcg tcagcggggt gaagccaccc tccatggcat
     2641 cgcgcaggca catcaggccg accacttccg tgtgatcggt gtgagggttg agctcgttgc
     2701 ggttctggta ggcccgggcg ttgggatcga tgtgggtcat atcgcggatc tggccaaggc
     2761 gatcgccctt ggcgctctgg ggcacggccg cgccgatcga tcggccaagg ccccagaaga
     2821 tcagttgcaa gtcgtccttg ccgaaccggt cggttggcag tcccgtcagc agggccagcc
     2881 ccgatccgtc gcgaagttcg gccaggacct gcttcatccg gcgatcgacc agcggccagt
     2941 cgaacaggtt gtcgggcgcg gtctcagccg tgatgccgcg ctggcgggcc tggtccgtca
     3001 cacgctccat ttccgcgatc tcgtccttcg agaagggaga gccgaggccg gccggatcgc
     3061 ccaaatcagc ggcagtccac accgctttgc cggtcgcggg ccggatcgtt tcgggcatgg
     3121 tcggaactcc tcaatggtgc catttatgag tttgcggcct agattgcgac ttggctgccc
     3181 gctgtccatg gtctggcgca gcctcccggg caggccgcag agccatcacg cgtatgatcc
     3241 tcgtctcccg catgaccggg cttcgcccgt ccccggccat tccttgcctc acgccgaaac
     3301 ggggcccact gcaaatcttg gttaaggaat ttccactaga gacgatcccc tgggccgccg
     3361 ccacggggga agggcggcca attggaaaac cgcaaagccg cttcatgatc cgcagcaaga
     3421 cgacctttgt cctcgggccc ggcgccgcgg cggaactgca ttttccgggc gcggccgaga
     3481 cgcttgagcg gatcgccgcc gcgctcgatt tcagccgcgc cacggccaac cagatgaccc
     3541 gcgatacggc ggcgatcctg cgccatgtcg gcaagctggc cgaacgcacc gggcgcagcg
     3601 aggagcagct ctaccgcgcc gcccagcgca tccaccagtc ggccaaggtc ggccgcacgg
     3661 tcgaaggggt gatcgatcag aacgacgacg atccgctggt ggaggccttc ggcaagctgg
     3721 ccctggccgt attcacgctc cagtccgaac agcgctcctc ggtccgcgcc gcgccccagg
     3781 tggccggagg cctgccaatg cagaccggcg attattggct gaccgaactc ggccgcctga
     3841 tctgcgccag cctgccgcgc tccaggctgg agcagggact ggccgatgta agcctggtct
     3901 ccttcaccta tgaccgctcg gccgaacacg tgctgcccta tgtcgtttcg accgcctacg
     3961 ggatgacgct ccaggaagcc cagcgcatgg tcgcctcccg gctcaaggtg ttccaccccc
     4021 ttggctcggt cggccgcctg ccgtggcagt ccggcgacgg cccggatgtc gactggggca
     4081 acgagcaggc ctggaacatc gcggccctgt cggcccacat ccgcactgcg ggccaggcga
     4141 tgaaggaccg ggtggcgctc ggcgccctgc gcgcggccgt ggcggcctcg tcgcggatcg
     4201 tgttcctcgg cttcgatttc gcccccgccg cgcttgacct gatggtcgac ggcagcctct
     4261 cgcacaatcc cgagctggtg atctgcgccc cgggcctcgt cggccccaac cgtgatgcgg
     4321 cgatccgcat gctcaagcgc aagaccgggg tggaacgcga tgaccgcctg atcttcgcgg
     4381 agggccgctg cctcgacctg ctcaaggact atacgctgct gctggaaagc tgagcattcc
     4441 ttatcttccc cgttgatggg ggtgatgctt cacgcaggca cagccttacg ccgcccccgt
     4501 ctccagccat tcgcccagcg cggccaggca atcgcgtgcc agctggcggc tgcgcgcggg
     4561 gctccagccg tgcatcggat cgggcaggtc gtcattgtcc ttgaacggca tttccagggt
     4621 catggaaacg cacccatggc tgcggccaaa gcgctcggcc agctggttgg tgctcatggc
     4681 aaggttggcc gcgcccggcc gggccaccgg gtagcccagg cgggtctgga aatcgggcgt
     4741 gcgccgctcg aggatgcggc ggtagcgggc atagccttgc
//
LOCUS       DATNBV010000108         4523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2619682,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000108 DATNBV010000000
VERSION     DATNBV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619682"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VN627_02630"
     CDS             <1..526
                     /locus_tag="VN627_02630"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HXD02181.1"
                     /translation="GRAGAHRQRQRRTAVGHAGGGALAPAGRLMGNFSRYALVALVAF
                     AMALAASWLGRSLFEPRHAQGGELHVLMHEQLDLDPAQKQKIAALEADFAEQRKVLDA
                     RLREANAGLAQAMVSEHQYGPRVAEAVDRCHMAMGELQKATLRHVFAMRAVLRPDQAA
                     RFDAAISKALTRPA"
     gene            531..1091
                     /locus_tag="VN627_02635"
     CDS             531..1091
                     /locus_tag="VN627_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXD02182.1"
                     /translation="MAGGDEADDAALVAQALAGRQMAFTTLMARHREAVYRLARNHVG
                     GEADALDVTQETFIAAFAALGRYDTARPLRGWLLRIALNKCHDWARRRAVRRLFTFAR
                     PLDDALDVADAAPDQERALGSARGVTKIRAAIAALPAQLKEPLILCAIEGLSQDEAAE
                     VLGTSRKAVETRIYRARQKLSAALEG"
     gene            1118..2863
                     /locus_tag="VN627_02640"
     CDS             1118..2863
                     /locus_tag="VN627_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HXD02183.1"
                     /translation="MDRSLTLDRRALLRTGGSLAASFALAPLFPAWAQSGTPGAGASI
                     IGGTTRLEIAPGHSSVGGRSLHHAVTVNGTIPGPLIRLKEGQTARIAVSNGLAEQTSI
                     HWHGVLVPFQMDGVPGLSFPGIDPLETFTYEFPLIQSGTYWYHSHSGMQEAVGLYGPL
                     IIDPAGPDPIVADREHVLMLADWSPMHPHEQLRRLKVQAGYFNRQRQDIAGLLARRDQ
                     PLKERLEWAKMRMDPTDISDVTGFTYSFLINGQDTQANWTGLFKPGERVRLRIINASA
                     MTNFNFRIPGLALTIVAADGNLVQPVETDELQIAIAETYDIIVTPTQDKAFGVIAEAL
                     DRSGQVRGTLAPRMGMAGEVPPLRKRPLLTMRDMGMDMSGMDMGQNGEIDLSKPASNS
                     MAGHSMNMRDKSVAPQVRYGPQVATISPSPADRLADKPTGLEDVDHRVLTYADLRSLA
                     PNPDPRKPSRQIDVHLTANMERYVWSIDGETISGGAAPIPFRENERVRVNLINDTMMP
                     HPIHLHGHFFELVSGDPGHRVRKHTVNVLPGGKVAFDLTADAPGDWAFHCHMLLHMHA
                     GMMRVVTVRRDGENA"
     gene            2863..3771
                     /locus_tag="VN627_02645"
     CDS             2863..3771
                     /locus_tag="VN627_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HXD02184.1"
                     /translation="MRGLALLAVVPLALAAPALAQEHRHDMSGMDHSGHDMAGMDMPD
                     NATPTAAPAADDTPGNAAPPPVPADHPADRFFPQARTEAARAGLMSEGRWTGSAVMVD
                     RLELRTGSGHEGYAWKGGAWYGSDLNKLVLATEGEGLFGESAERIEVQALWRHALDAW
                     FNLEAGVRQDFRPVPRRTYAVLGVEGLLPWWIEGEAQLYVSHKGDVHARLSASHDLRL
                     SGPLVLQPEVEVNFAFQDVPQLGVGSGLERIETGARLRYEIRPELAPYIGMKWERSFG
                     GMARATRGAGERASAVTAVAGLRALF"
     gene            3949..>4523
                     /locus_tag="VN627_02650"
     CDS             3949..>4523
                     /locus_tag="VN627_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD02185.1"
                     /translation="MNPLDGRIALVTGASRGAGRGIALALGEAGATVYITGRTAAASD
                     SRDEVMGRPIPGSLAETAAEIRAAGGKCVPVACDHGDDGQVARLFRQIEDEAGRLDIL
                     VNNAYQPHHNTSNAVRFWEADGPESWDSQMTVGLRSAFVASQHAARIMVRQRSGLIVA
                     ISSPSAAGYILEIPYCITKAALDRLSADMAH"
BASE COUNT          733 a         1548 c         1546 g          696 t
ORIGIN      
        1 cgggcgcgct ggcgcccacc gccagcgcca gcgccgaacc gctgtggggc atgccggcgg
       61 cggcgccctc gcacctgctg gtcgattgat gggcaatttc tcccgctatg cgctggtggc
      121 gctggtcgcc tttgccatgg cgctggcggc gagctggctg ggacgcagcc tgttcgagcc
      181 gcgccatgcg caaggcggcg aactgcacgt gctgatgcac gaacagctcg acctcgatcc
      241 agcgcagaaa cagaagatcg ccgcgctgga ggccgatttc gccgagcagc gcaaggtgct
      301 cgatgcgaga ttgcgcgaag ccaatgccgg gctcgcccag gccatggtga gcgagcacca
      361 gtatggcccc cgcgtcgccg aggcggtcga tcgctgccac atggcgatgg gcgaactgca
      421 gaaggcaacc ctgcgccatg tctttgccat gcgtgccgtg ctgcggccgg accaggccgc
      481 gcggttcgat gccgcgataa gcaaagcgct gacccggccg gcctaggcga atggcagggg
      541 gcgacgaggc agacgacgcc gcgctcgtcg cgcaggcgtt ggccgggcgg cagatggcct
      601 ttaccacctt gatggctcgt caccgagagg ccgtgtaccg cctcgctcgc aaccatgtcg
      661 gcggcgaggc cgatgcgctc gacgtcacac aggaaacctt cattgcggcc tttgcggcac
      721 ttggccgcta tgacacggcg cggcccctgc gcggctggct gctgcggatc gcgctcaaca
      781 agtgccacga ctgggcgcgc cgccgcgcgg tacgccgcct gttcaccttt gcccgtccgc
      841 tcgacgatgc gctggacgtg gctgatgctg cgcccgatca ggaaagggcg ctgggctccg
      901 cgcggggagt caccaagatc cgcgccgcaa tcgccgccct gccggcgcag ctgaaggagc
      961 cgctcatcct gtgcgcgatc gaggggctca gccaggatga ggcggcggag gtgctcggca
     1021 ccagccgcaa ggcggtggag acgcggatct accgggcgcg gcagaaattg tcggcggcgc
     1081 tggagggata ggccgagggc gcgcgtatag gagggcgatg gatcgttctc tcacgcttga
     1141 tcgccgcgcc ctgttgcgca ccgggggctc gctggccgcc agcttcgccc ttgctccgct
     1201 gttccccgcc tgggcgcaga gcggcacccc gggcgcgggc gccagtatta tcggtggtac
     1261 gacacggctg gagattgcgc cgggacactc ttcggtcggc gggcgctcgc tgcatcatgc
     1321 ggtgaccgtg aacggcacga tccccggccc gctgatccgg ctgaaggaag ggcagaccgc
     1381 ccgcatcgca gtgagcaatg ggctggccga gcagacctcg atccactggc acggcgtgct
     1441 ggtgccgttc cagatggacg gcgtgcccgg gctgtcgttc cccggcatcg atccgctcga
     1501 gaccttcacc tacgaatttc ctctgatcca atcggggacc tactggtacc acagccattc
     1561 gggcatgcag gaggcggttg gcctctacgg gccgctgatc atcgatccag ccgggcccga
     1621 tccgatcgtg gccgaccgcg agcacgtgtt gatgctggcc gactggagcc cgatgcaccc
     1681 gcacgagcag cttcgccgcc tcaaggtcca ggccggctat ttcaaccgcc agcggcagga
     1741 cattgccggt ctcctggcca ggcgcgacca gccgctgaag gaacgcctgg aatgggcaaa
     1801 gatgcggatg gaccccaccg atatctcaga tgtcaccggc ttcacctatt cgttcctgat
     1861 aaacgggcag gatacgcagg ccaattggac cggcctgttc aagcccggcg aacgggtgcg
     1921 gctgcggatc atcaatgcct cggcgatgac caacttcaac ttccgcatcc cgggcttggc
     1981 gctgacaatc gtcgcggctg acggcaatct ggtccagccg gtcgagaccg acgaactgca
     2041 gatcgccatt gccgagactt acgacatcat cgtcacgcca acgcaggaca aggcctttgg
     2101 cgtgatcgcc gaggcgctcg accgctccgg ccaggtgcgc ggcacgctgg ccccgcgcat
     2161 gggcatggcg ggcgaggtcc cgccgctcag gaagcgcccg ctgctgacca tgcgcgacat
     2221 gggcatggac atgtccggca tggacatggg ccagaacggc gagatcgacc tctccaagcc
     2281 ggccagcaat tccatggccg gccattccat gaacatgcgc gacaagagcg tggcgccgca
     2341 ggtcaggtac ggacctcagg tcgccacgat ctcgccctcg ccggccgatc gcctcgccga
     2401 caagccgacc gggctggaag acgtggacca ccgcgtcctc acctatgccg atctgcgcag
     2461 ccttgcgccc aaccccgacc cgcgcaaacc gagccgccag atcgacgtgc accttaccgc
     2521 caacatggaa cggtatgtgt ggtccatcga cggcgagacc atttccgggg gcgccgcgcc
     2581 gatccccttc cgcgagaacg agcgtgtgcg ggttaacctc atcaacgaca ccatgatgcc
     2641 gcatccgatc cacctccacg gccacttctt cgagctggtc agcggggacc cgggccaccg
     2701 cgtgcgcaag catacggtca acgtcctgcc gggcggcaag gtcgccttcg accttaccgc
     2761 cgatgcgccc ggagactggg cctttcactg ccacatgctg ttgcacatgc atgccggaat
     2821 gatgcgcgtc gtcaccgtcc gccgcgacgg ggagaacgcc tgatgcgcgg ccttgccctg
     2881 cttgccgtcg tgccgctggc gctggcggcg ccagctctcg cgcaggagca tcggcacgac
     2941 atgtcgggca tggaccattc cggtcacgac atggccggga tggacatgcc ggacaatgcc
     3001 acccctaccg ccgcgccggc tgccgacgat acgcccggca atgccgcgcc tccgccggtg
     3061 ccggccgacc acccggcgga ccgcttcttc ccgcaagcgc ggacggaggc cgctcgcgcc
     3121 ggactgatga gcgagggccg gtggaccggc agcgcggtga tggtcgaccg gctggaactc
     3181 cgcaccggtt ccggccacga gggctacgcc tggaaaggcg gcgcctggta cggcagcgac
     3241 ctgaacaagc tggtgctcgc cacggagggc gagggcctgt tcggcgagag cgccgagcgg
     3301 atcgaggtcc aggcactgtg gcgccacgcg ctcgacgcct ggttcaacct cgaggccggc
     3361 gtccggcagg acttccgccc cgtgccgcgc cgcacctatg ccgtgctcgg cgtggagggc
     3421 ctgctgccct ggtggatcga gggtgaggcg cagctctatg tctcgcacaa gggcgatgtc
     3481 cacgcccgcc tgtcggcaag ccatgacctg cgcctgtcgg gtccgctggt gctccaaccg
     3541 gaagtggaag tgaacttcgc cttccaggat gtgccgcagc ttggcgttgg ttcgggcctt
     3601 gagcggatcg agaccggcgc ccgcctgcgc tacgaaattc ggcccgagct cgcgccctac
     3661 attggcatga agtgggagcg gagcttcggc ggcatggcac gggccacgcg cggtgcgggc
     3721 gagcgcgctt cggcggttac ggccgtggcg gggctgcgcg ctctcttctg acgccaggct
     3781 ggtccgtcgc cggcgccgtg gtgggggaac gccgagccgg tgaccggagc ggggtcatgg
     3841 tgggggcgcg gtatccgcca cagccatgat gaaaccggcg gtgccttgcg ggaagccggt
     3901 gcgccccgca caacgcgaca aaatccagtg gagagaggag cctggatcat gaatccactc
     3961 gatggccgca ttgctctcgt cacgggcgcc agccgcggag ctggcagggg catcgccctc
     4021 gccctgggcg aagcgggtgc cactgtttac atcaccgggc gaaccgccgc ggcgtcggat
     4081 tcgcgggacg aggtcatggg ccggccgatc cctggttcgc tggcggaaac cgccgcggaa
     4141 atccgggccg ccgggggcaa atgcgtgccc gttgcctgcg atcacggcga cgatggccag
     4201 gtcgcccgcc tgttccggca gatcgaggac gaggccgggc ggctcgatat cctcgtcaac
     4261 aacgcctatc agccgcacca caacacctcg aacgccgtcc gcttctggga agccgacgga
     4321 cccgaatcgt gggatagcca gatgacggtc ggcctgcgct cggcgttcgt tgccagccag
     4381 cacgccgcgc ggatcatggt gaggcagcgc agcggtctga tcgtggcgat ctcgtcgccc
     4441 tcggccgcag gctacatcct cgagattccc tactgcatca ccaaggccgc gctcgatcgc
     4501 ctctcggccg atatggccca tga
//
LOCUS       DATNBV010000109         2129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4211851,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000109 DATNBV010000000
VERSION     DATNBV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4211851"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VN627_02655"
     CDS             complement(<1..324)
                     /locus_tag="VN627_02655"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXD02186.1"
                     /translation="MRGRTDLRVLIASFALLATACQAGPRDMKSSTEAYGSAEKQVLD
                     IYWTGEGPPAPLILFIHGGGWSMGSKKDGESGQPGYFVTQGYAYASLDYRLVPDVKVE
                     DQLSDV"
     gene            400..1689
                     /locus_tag="VN627_02660"
     CDS             400..1689
                     /locus_tag="VN627_02660"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HXD02187.1"
                     /translation="MANVTVIGAQWGDEGKGKIVDWLAERADVVVRFQGGHNAGHTLV
                     VGNQTYKLSLLPSGIVRGTLSVIGNGVVLDPWHFREEVGKLKNQGVTMTPDNLQVAET
                     CPLILPFHRDLDGLREDASGAGKIGTTRRGIGPAYEDKVGRRAIRVCDLAHLDDLELQ
                     LDRICAHHDALRAGFGEGPIDRERLMADLRDIADFILPFAKPVWLTLNQAKARGDRIL
                     FEGAQGVLLDVDHGTYPFVTSSNTIAGTAAGGSGLGPSAAGFVLGIVKAYTTRVGSGP
                     FPTELTDETGQRLGERGHEFGTVTGRKRRCGWFDAVLVRQSVAVSGVTGVALTKLDVL
                     DGFAELNICTGYTVNGHHYDYLPPHPQDQARAEPVYETIEGWSQSTAGARSWAQLPAQ
                     AIKYVRRIEELIGCPVALVSTSPEREDTILVRDPFAD"
     gene            1699..>2129
                     /locus_tag="VN627_02665"
     CDS             1699..>2129
                     /locus_tag="VN627_02665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXD02188.1"
                     /translation="MALEDPGFVAVLAEYNERLAREDPIVHDPQALQAADVNDLLIPV
                     GEDVGRLMHALVVGLGAHIIVELGTSYGYSTLFLADAAKVTGGKVYSYDVAANKQAYA
                     RERLQRAGLSDYVEFRLGDAVELLEGQPGPVDFVLMDLWKDL"
BASE COUNT          387 a          663 c          681 g          398 t
ORIGIN      
        1 aacgtcggag agctgatcct cgaccttcac atcgggcacg aggcgatagt cgagcgaggc
       61 ataggcatag ccctgcgtta cgaaatagcc cggctgcccg ctctcaccgt ccttttttga
      121 tcccatcgac cagccgccgc catggatgaa aaggatcagc ggcgcgggcg gcccctcgcc
      181 tgtccaataa atatcgagca cctgcttttc cgcgctgcca taggcttcgg tgctgctctt
      241 catatcgcgc ggcccggcct ggcaggcggt ggccagcagc gcgaacgagg cgattaggac
      301 acgcagatcg gttctcccgc gcatgattcg gcacataggc atggacgcga aggcccgcat
      361 ccaataggca gcgcccgctt gcaagcaaag gggagccacg tggccaatgt aaccgtcatc
      421 ggtgcccaat ggggcgatga aggcaagggt aagatcgtag actggctggc ggagcgcgcc
      481 gacgtcgtgg tgcgcttcca gggcgggcat aatgctggcc atacgctcgt cgtcggcaat
      541 cagacctaca agctgagcct gctgccctcg ggcatcgtgc gcggcacgct ctcggtgatc
      601 ggcaacggtg tcgtgctcga tccttggcat ttccgcgaag aggtcggcaa gctcaagaat
      661 cagggcgtta cgatgacacc cgacaacctg caggtggccg aaacctgccc gctgattctt
      721 cccttccatc gcgatctcga cgggctccgg gaggatgcct cgggcgccgg caaaatcggc
      781 accacccggc ggggcatcgg cccggcctat gaggataagg tcggccgccg tgcgattcgg
      841 gtttgcgacc ttgcccatct cgatgatctc gagcttcagc tcgatcgcat ctgtgcgcat
      901 cacgatgccc tgcgtgccgg gttcggcgaa ggaccgatcg atcgcgagcg gctgatggcc
      961 gacctgcggg acatcgccga tttcatcctg cccttcgcca agccggtctg gctcacgctt
     1021 aaccaggcca aggcgcgcgg cgaccgcata ttgttcgaag gcgcccaggg tgttcttctg
     1081 gacgtcgatc acggcaccta tccgtttgtc acctcttcca acaccatcgc aggcacggct
     1141 gccggcggct ccggcctcgg cccgagcgcg gcgggcttcg tgctgggcat tgtgaaggca
     1201 tatacgaccc gcgtcggttc cgggcccttt ccgacggaac ttaccgacga aaccgggcag
     1261 cggctcggcg agcgcggcca tgagttcggc acggtgaccg ggcgcaagcg ccgctgcggc
     1321 tggttcgatg cggtgctggt gcgccagtca gtggcggtgt caggtgtgac cggcgtagcg
     1381 ctcaccaagc tggacgtgct tgatggtttc gctgagctca acatctgcac gggctatacg
     1441 gtcaatggcc accactatga ttatctgccg ccccatccgc aggatcaggc gcgcgccgag
     1501 cccgtttacg aaacgatcga gggctggagc caatcgaccg ccggcgcccg cagctgggcg
     1561 caactgcctg cacaagccat caaatatgtg cgccggatcg aggaattaat cggatgcccg
     1621 gtcgcgctgg tatcgaccag ccccgagcgg gaggatacga tcctcgtgcg ggaccctttc
     1681 gctgattgag gagcaaccat ggcgctcgaa gaccctggtt tcgtagccgt actcgccgaa
     1741 tataacgagc ggctggcgcg cgaggatccg atcgtgcacg atccccaagc cctccaggca
     1801 gcagacgtga atgatctcct gattccggtc ggcgaggacg tcggaaggct catgcatgcg
     1861 cttgtcgtcg ggctcggcgc gcacatcatc gtcgagcttg gcacgtctta cggctactcg
     1921 accttgttcc tcgcggacgc ggcgaaggtg acgggcggca aagtctatag ctacgacgtt
     1981 gcggccaaca aacaggccta tgctcgcgag cgactgcagc gcgcaggctt gagcgattat
     2041 gtcgagttca gattgggtga tgccgtcgag ctgctcgaag gtcagccggg acctgtcgat
     2101 ttcgtgctga tggatttgtg gaaggatct
//
LOCUS       DATNBV010000110         5448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1655183,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000110 DATNBV010000000
VERSION     DATNBV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655183"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VN627_02670"
     CDS             complement(<1..494)
                     /locus_tag="VN627_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02189.1"
                     /translation="MSGRATPPRANRRLRLDRALGVALVALAVPAGMAMAPPASAQSP
                     RFHAPQGPVVLTREVRRTLADGQQLVVRRRYLVRFVPTASGYTVTGNPAGVEVEAPPA
                     MAELAEVERRRADLGLFPLQLDAAGLIVARPAPTESVEATRARAAMSAYLARTDLTAQ
                     ERTAA"
     gene            complement(615..1673)
                     /locus_tag="VN627_02675"
     CDS             complement(615..1673)
                     /locus_tag="VN627_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HXD02190.1"
                     /translation="MQGTAQALDGRSQGSTSRERPLALARWLLVIAALVVAIVAVGGI
                     TRLTESGVSITEWKPVSGAIPPLTQAQWQVEFDAYRQTPQYIEINGPAGMTLATYKMI
                     FFWEWVHRLLARAIGLALFGVLAWFGLRREIPSGYGPRLAALVALVGLQGAIGWWMVQ
                     SGIVHDVKVSHFRLAAHLLTALFTLGGLVWTALDLLAHARGEAPRRLTGFGLLALAVL
                     ALQLFMGALVAGLRAGHVASDWPLMQGRLFPEGVDWSQGPLHAVLSDPFLTHFDHRWW
                     AWMVVAVLVVMARRLRRAGARQSGIAIHSAFGTQVILGIATVMSGIALWLAVAHQLVG
                     ALLVAATVWGAHELGRRA"
     gene            1806..2177
                     /locus_tag="VN627_02680"
     CDS             1806..2177
                     /locus_tag="VN627_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HXD02191.1"
                     /translation="MRDALLAIRDRLDGWGVALSAACAVHCVVGLVLVTFLGLGGGVL
                     LSPSVHRIGLALAVCIGLVTLGMSALRHGNLTPALIGACGLLLMTAGLFAPHGPAEAL
                     LTIPGVGLVAFAHIRNLRHAH"
     gene            2197..3183
                     /gene="thiS"
                     /locus_tag="VN627_02685"
     CDS             2197..3183
                     /gene="thiS"
                     /locus_tag="VN627_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HXD02192.1"
                     /translation="MTEIALTLNGEPRRAPAGSSIAAFLELLGFNPAKVAVERNGDII
                     PRATLGEVVLGEGDVLEVVHFVGGGEAMNDDTWTVAGRTFRSRLIVGTGKYKDFAQNA
                     AAVAASGAEIVTVAVRRVNVSDPSAPMLTDFIDPRQITYLPNTAGCFTAEDAIRTLRL
                     AREAGGWDLVKLEVLGEARTLYPDMRETLKATEVLAKEGFLPMVYCADDPIAARQLEE
                     VGAVAIMPLGAPIGSGLGIQNKVMIRLIVEGAKVPVLVDAGVGTASDAAVAMELGCEG
                     VLMNTAIAEAKDPVLMAHAMKLAVEAGRAAYRAGRMATRRYADPSSPLAGLI"
     gene            3421..3948
                     /locus_tag="VN627_02690"
     CDS             3421..3948
                     /locus_tag="VN627_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02193.1"
                     /translation="MRNTATASLAIAEFREFASFSPCEQRYIKRSLDVGLGRQDAFKL
                     WARDGAEMASIRSQYVAYQDLKVLRCDPPVETSLDGVDGYVGKLVRVSAFDLGQGRLG
                     CFSAYRFLYERLIGPMVRPWLPAAFCGAAALPQIRPDLRKQLLQSISEAAATAPGWSP
                     TAPRFYPEWVEQEAA"
     gene            complement(3945..>5448)
                     /locus_tag="VN627_02695"
     CDS             complement(3945..>5448)
                     /locus_tag="VN627_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HXD02194.1"
                     /translation="AVIVADARLAKPLLPAVLRSDTVAHVIAIEGFKLPQLGQADCHD
                     WAALITGDAAAARRAVDARIAGIGREDLACLIYTSGTGGAPRGVMQHHGMILRNIDGA
                     AQVLAEDFGWDASGRRPEVFLSFLPLSHAYEHTGGQFLPIGMGAQIWYAEGLDKLAGN
                     IEEVRPTFMIVVPRLFEVLRTRIMKGIEKQGRVANFLLDRALKIESRAGEHGKRLVDR
                     PLDLIIARLLRPKVRARFGGRMKAMVSGGAPLNPEIGTFFAAMGLTLLQGYGQTESGP
                     VVSCNRPRVGLKMETVGPPLDKVEVRIAADGEILVRGELVMQGYWRNPEETAKVLKNP
                     GAPPGAGVWLHTGDIGHFDDKGRIVITDRKKDMIVNDKGDNVSPQRVEGMLTLQPEIA
                     QAMVVGDRRPWLVGLIVPDAEWALAWARAQDEKFDMAALQDLPAFRSAIRAAIDRVNS
                     ELSVTEKVRQFAFADEPFTIENEEMTPSLKIRRHKLKERYGARLDALYKA"
BASE COUNT          823 a         1800 c         1963 g          862 t
ORIGIN      
        1 gcggcggtgc gttcctgggc ggtgaggtcg gtccgggcga ggtaagcact cattgcagcc
       61 ctggcgcggg tggcctcgac ggattctgtc ggggccggcc gggcgacgat cagcccggcg
      121 gcatcgagct ggagcggaaa caggccaagg tcggcgcggc ggcgttcgac ctcggccagt
      181 tcggccatgg cgggcggggc ctcgacctcg accccggcgg ggttgccggt gacggtatag
      241 ccgctagcgg tgggcacgaa gcggacgagg tagcggcggc ggacaacgag ttgctggcca
      301 tcggcgaggg tgcggcgcac ctctcgggtg aggaccaccg ggccctgcgg ggcgtggaag
      361 cgcggcgact gggccgaggc gggcggggcc atggccatgc cggcgggaac ggccaacgcg
      421 acgagcgcga cgccgagcgc gcgatcgagg cgcagccgcc ggttcgcccg tgggggagtg
      481 gctctcccgc tcatgcctgg tcccccgtca tgccggcacc ggtccacgtc cgccgcgaat
      541 cgccggatcg taaccgagca tatacgcaga aatccttgcg atttgcttgt cgccgaaggc
      601 ggggcggtaa ccgttcatgc gcggcggccc agttcgtgcg cgccccagac cgtggcggca
      661 acaaggaggg cgccaaccag ctggtgggcc acggcaagcc acagggcgat gccgctcatg
      721 accgtggcaa tgccgaggat cacctgcgtg ccgaaggccg aatggatcgc gattcccgac
      781 tggcgggctc ccgcgcggcg caggcggcgg gccatgacca ccagcacggc caccaccatc
      841 caggcccacc agcgatggtc gaaatgggtg aggaacggat cgctgaggac ggcgtgcagg
      901 gggccttgcg accagtccac cccctcggga aacaggcggc cctgcatcag gggccagtcg
      961 ctggcgacgt gcccggcccg cagcccggcg acgagcgccc ccatgaacag ctggagggca
     1021 aggaccgcaa gggcgaggag gccgaagccg gtcaaccggc ggggcgcttc gccgcgggcg
     1081 tgggcgagga gatcgagggc ggtccagacc aggccgccga gcgtgaacag cgcggtgagc
     1141 aggtgggcgg cgaggcgaaa atggctgacc ttgacgtcgt ggacgatgcc cgactggacc
     1201 atccaccagc cgatcgcgcc ctgcagcccg acaagggcga ccagcgcggc gaggcgcggg
     1261 ccgtagccgc tcgggatctc gcggcgcagg ccgaaccagg cgagcacgcc gaacagggcc
     1321 aggccgatgg cgcgggccag gaggcggtgg acccattccc agaagaagat catcttgtag
     1381 gtggccagcg tcatccccgc agggccgttg atctcgatat attgcggggt ctgccggtag
     1441 gcatcgaact cgacctgcca ctgcgcctgg gtcagcggcg gaatggcccc gctgaccggc
     1501 ttccactcgg taatggagac gccgctctcc gtcagccggg tgatcccgcc gaccgcgacg
     1561 atagccacga ccagcgccgc tatgaccagc agccagcgag ccagcgctag cggccgttcc
     1621 cgggaggtgg agccttgcga acggccgtcc agcgcctgtg cggtaccttg catgagcccg
     1681 gcgatgcgcc gcaccgggga aaatcgcaag aggtgttgta aactccaccg acgttcgggc
     1741 aggcgcgaga accactacgc gcccaaccct tgaacgatgt tacaacatca catacatcgc
     1801 ctcccatgcg tgatgccctc cttgcgattc gggaccggct ggacggctgg ggcgtggccc
     1861 tttcggccgc ctgtgcggtg cattgcgtgg tcggtctggt gctggtgacc ttcctcggcc
     1921 ttggcggcgg cgtgctgctt tcgccgagcg tgcaccggat cgggctggcg ctggccgtct
     1981 gcatcggcct ggtgactttg ggcatgtcgg ccctgcggca cgggaacctg accccggcgc
     2041 tgatcggggc ctgcggactg ctgctgatga ccgcgggcct gttcgccccg catggcccgg
     2101 ccgaggcgct gctgaccatt cccggcgtgg ggctggtcgc ctttgcccac atccgcaact
     2161 tgcgccacgc gcactgaacg ctattgcccg tcggcgatga ccgaaatcgc cctcaccctc
     2221 aacggcgagc cgcgccgcgc gcccgccggc agttcgattg ccgcgttcct tgaactgctc
     2281 gggttcaacc cggcaaaggt ggcggtggag cgcaatggcg atatcatccc gcgcgcgacg
     2341 ctgggcgagg tggtgctggg cgaaggcgat gtgctggaag tggtccattt cgtgggcgga
     2401 ggtgaagcga tgaacgatga tacctggacc gttgcgggcc gcaccttccg ttcgcgcctg
     2461 atcgtcggca ccggcaagta caaggacttc gcccagaatg cggcggcggt ggctgcctcg
     2521 ggcgcggaga tcgtcacggt cgccgtgcgg cgggtcaatg tttctgatcc ttcggcgccg
     2581 atgctgacgg acttcatcga tccccggcag atcacctatc tgcccaacac tgccggctgt
     2641 ttcaccgccg aggatgcgat ccgcacgctg cggctggcgc gcgaggcggg cggctgggat
     2701 ctcgtcaagc tcgaggtgct gggcgaggcg cgcacgctgt atcccgacat gcgcgagacg
     2761 ctgaaggcga ccgaggtcct ggcgaaggaa ggcttcctgc cgatggtcta ctgcgccgac
     2821 gacccgatcg ccgcgcgcca gctggaggaa gtgggcgccg tggcgatcat gccccttggc
     2881 gcgccgatcg gctcgggcct gggcatccag aacaaggtga tgatccggct gatcgtcgaa
     2941 ggggccaagg tgccggtgct ggtcgatgcc ggcgtcggca cggcgtcgga tgcggccgtg
     3001 gccatggagc tgggctgcga gggcgtgctg atgaacacgg ccatcgccga agcgaaggac
     3061 ccggtgctga tggcccatgc gatgaagctg gcggtcgagg ccgggcgcgc ggcctatcgg
     3121 gcgggccgga tggcgacgcg gcgctacgcc gatccgtcga gcccgctggc ggggctgatc
     3181 tgacacgatt ccccctctcg cctgcgggag gggcagtgag acttgcagcg ccgcgggcgg
     3241 tgcctagtcg caggtgggca gccggcaagc gccgcgccgg cccgcttgcc cacccccgcc
     3301 ccctctcgca ggcgggaggg gagtgacacc gtggctcttg atgtcattaa ccccattcta
     3361 accatgcccg tgccagtttg gccgatgcgg gaatcgcccc gcttcgggat caccagcgcc
     3421 atgcgcaaca ccgccacagc ctcgctcgcc attgcggaat tccgcgagtt cgcctcgttc
     3481 tcgccgtgcg agcagcgcta catcaagcgc agcctcgacg tgggcttggg gcggcaggac
     3541 gcattcaagc tgtgggcgcg cgacggggcg gagatggcct cgatccgcag ccagtatgtt
     3601 gcttaccagg acctgaaggt cctgcgctgc gaccccccgg tggaaacttc gctcgacggg
     3661 gtcgatggct atgtcggcaa gctggtccgg gtgagcgcct tcgatctggg ccagggccgg
     3721 ttggggtgct tttcggccta tcgctttctc tacgagcggc tgatcgggcc gatggtgcgg
     3781 ccgtggttgc cagccgcctt ctgcggcgcg gcggccctgc cccagatccg gccggacctg
     3841 cgcaagcagt tgctccagtc gatcagcgaa gccgcggcga cggctccggg ctggtcgccc
     3901 acggcgccgc ggttttatcc cgaatgggtc gaacaggagg ccgcctaggc cttgtacagg
     3961 gcgtccagcc gggcgccgta gcgttccttc agcttgtgcc ggcggatctt gaggctgggg
     4021 gtcatttcct cgttctcgat ggtgaagggc tcatccgcga aggcgaactg gcggaccttt
     4081 tcggtcaccg agagctcgct gttcacccgg tcgatcgccg cgcggatcgc gctgcggaag
     4141 gcgggcaggt cttgcagcgc ggccatgtcg aacttttcgt cctgcgcccg ggcccaggcg
     4201 agcgcccatt ccgcatcagg cacgatcagt ccgacgagcc acggccgccg gtcgcccacg
     4261 accatggcct gggcgatctc cggttgaagg gtgagcatgc cttcgacgcg ctggggcgag
     4321 acattgtcgc ctttgtcgtt gacgatcatg tccttcttgc ggtcggtgat gacgatccgg
     4381 cccttgtcgt cgaagtggcc gatgtcgccc gtgtgaagcc agacgcctgc cccgggcgga
     4441 gcgccggggt tcttcagcac tttcgccgtc tcttccggat tgcgccagta gccctgcatg
     4501 accagctcgc cgcgcaccag gatttcgccg tccgcggcga tgcgcacctc gaccttgtcc
     4561 agcggcgggc cgaccgtctc catcttgagg cccaccctgg ggcggttgca ggagacgacc
     4621 gggccgcttt ccgtctggcc gtagccctgc agcagggtca ggcccatggc ggcgaagaag
     4681 gtgccgattt ccggattgag cggggcgccg cccgatacca tggccttcat ccgcccgccg
     4741 aacctggccc gcaccttggg gcgcagcagc ctggcgatga tgaggtcgag cgggcggtcg
     4801 accagccgct tgccgtgctc gccggcccgg ctctcgatct tgagcgcgcg gtccaacagg
     4861 aagttggcga cgcggccctg cttctcgatc cccttcatga tccgggtgcg taggacctcg
     4921 aacaggcgcg gcacgacgat catgaaagtg gggcgcactt cctcgatatt gccggccagc
     4981 ttgtcgagcc cctcggcata ccagatctgc gcgcccatcc cgatggggag gaactggccg
     5041 ccggtatgtt cataggcgtg ggacaggggg aggaaggaaa gaaacacttc cggcctgcgc
     5101 ccgctggcgt cccagccgaa atcctcggcc aggacctggg ccgcgccgtc gatattgcgc
     5161 aggatcatgc cgtggtgctg catgacgccg cgcggggcgc cgccggtgcc gctggtatag
     5221 atcaggcagg cgaggtcctc gcgcccgatc ccggcgatcc gcgcatcgac cgccctgcgc
     5281 gcggccgcgg catcgccggt gatgagggcg gcccagtcat gacagtcggc ctgacccagc
     5341 tggggcagct tgaagccttc gatggcgatc acgtgggcga cggtgtcgct acgcaggaca
     5401 gccgggagca ggggcttggc gagcctggca tcggccacga tcaccgcc
//
LOCUS       DATNBV010000111         3010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1984745,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000111 DATNBV010000000
VERSION     DATNBV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984745"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1018
                     /locus_tag="VN627_02700"
     CDS             230..1018
                     /locus_tag="VN627_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXD02195.1"
                     /translation="MAEADDELVIEHEAGLAVVRINRPAQMNSLSMDLLGRMGVAIPA
                     LIADPAVRAIMLTGTGPRAFCAGADMGMMGGPSDPEQTLAGMKSWHHWMIALRETEKL
                     VIGAINGAAAGGGFGIAMVADLLVAADTAVFKGAFSTLGVAADFGLGFTLPRAIGEKR
                     AMEIIACDRRVSAAEAKALGMVAEVFPAESFAAEARAYALKLAAGSRGIQLSKRLMRL
                     GDAAAFAAYLETEARTQTEAFQTEDVREGVRAFQERRPPRFAGR"
     gene            1108..1941
                     /locus_tag="VN627_02705"
     CDS             1108..1941
                     /locus_tag="VN627_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD02196.1"
                     /translation="MSNSPEQSWPVALVTGAGGGMGQVIAARLADRYALALSEFREEP
                     LAQLVNMLAAQGVNPAATATGDLGSPQVLEPLMASCKGRLKAVVHTAGHSPQTGTWRQ
                     ILDINLVTTAKLLDAIEAQMEPGLAVVLIASMGRLVSPPAEGALGEVLENPLAPDFLD
                     RMEPFLGDEAQKPYSAYSITKMWVAREVNRRAISWGPRGARIMSISPGMIYTRMGTSA
                     VAEAGADVLLEQTPVRRWGTPMDIANVAEFILSDKASFLTGSDILVDGGTGQLIQALK
                     G"
     gene            complement(1966..2655)
                     /gene="yihA"
                     /locus_tag="VN627_02710"
     CDS             complement(1966..2655)
                     /gene="yihA"
                     /locus_tag="VN627_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HXD02197.1"
                     /translation="MSAPRPSGPSDARVSEEETLLAERIERAGKLFSGRVEFLLSAPQ
                     LKFLPDPTVPEIAFCGRSNVGKSSLLNALTGRKAIARASVTPGRTQELNYFEVGTPTQ
                     FRLVDMPGYGFAKAPPAVVEKWRRLIRDYLRGRVVLKRTLLLVDARHGIKPVDAEFMK
                     MLDESAVGYRVVLTKADKLKASELAEVTAGTEAEARKHVAAHPQVLVTSAEVKLGIDA
                     LRAAVLEDAGA"
     gene            complement(2624..>3010)
                     /locus_tag="VN627_02715"
     CDS             complement(2624..>3010)
                     /locus_tag="VN627_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HXD02198.1"
                     /translation="HQPFILWIKDLSAPDPLHILNLFGLLPFTPPAFLGIGVLAVILG
                     TTMFFQFKLNPAPMDDMQKQIFSIMPWMMMFVMAPFAAGLLIYWITSNLLTIAQQKYL
                     YARHPQLRAMVEKERADVGAAAKRAK"
BASE COUNT          504 a          935 c         1109 g          462 t
ORIGIN      
        1 atacggacaa gatcgacgag cgaggacatc ggacccgaga ggaactcggg ggccggcggg
       61 tcaatccggt cgggcgcggc gcggctcatg gcgggaagcc tagcggcaca atggttacca
      121 ggtggcaaat cgcgcctatc acaagcgcgc gggatgaagg ccgcccttgc ctttgacggc
      181 gcggcgggat cacaaggcgg cgaccaaccg ggcagcggga gacaggacag tggccgaggc
      241 tgacgacgaa ctcgtgatcg agcacgaggc gggcctcgcc gtggtgcgga tcaaccggcc
      301 ggcgcagatg aattcgctgt cgatggacct gctgggccgg atgggcgtgg caattcccgc
      361 gctgatcgcc gatccggcgg tgcgcgcgat catgctgacc ggcaccgggc cgcgcgcgtt
      421 ctgtgccggg gccgacatgg gcatgatggg cggcccttcg gacccggaac agacgctggc
      481 cggcatgaag agctggcacc actggatgat tgccctgcgc gagacggaga agctggtgat
      541 cggcgcgatc aacggcgcgg ccgcgggcgg cggcttcggc atcgcgatgg tggccgacct
      601 gctcgtcgcg gcggatacgg ccgtgttcaa gggcgcgttt tcgacccttg gcgtggccgc
      661 cgattttgga ctcggcttca ccctgccccg cgcgatcggc gaaaagcgcg cgatggagat
      721 cattgcctgt gaccgccggg tgagcgcggc cgaggccaag gcgctgggca tggtggccga
      781 ggtctttccg gccgagagct tcgccgccga ggcccgggcc tatgccctca agctggccgc
      841 cggatcgcgc ggaattcagc tgagcaagcg cctgatgcgc ctgggcgatg ccgcggcatt
      901 tgccgcctac ctcgagaccg aggcccgcac ccagaccgag gcgttccaga cggaggatgt
      961 tcgcgaaggg gtccgcgcct ttcaggagcg gcgaccgccc cggtttgccg ggcgctaggg
     1021 tcaggacata tatggaatga tgagccggca aggttgcgac acggatcgcg ccatggcact
     1081 gggccggcga acagagagga aggtcagatg agcaattcac cggaacagtc gtggcccgtg
     1141 gcgctggtga ccggcgcagg cgggggcatg ggtcaggtca tcgccgcgcg actggccgac
     1201 cgttatgccc tggcgctgag cgaatttcgc gaggagccgt tggcgcaact ggtgaacatg
     1261 ctggcggcgc agggggtcaa tcctgccgcg accgccaccg gcgatctcgg cagtccgcag
     1321 gtgctggaac cgctgatggc cagctgcaag gggcggctga aggccgtggt ccacacggcc
     1381 gggcattcgc cgcagaccgg cacctggcgg cagatcctcg acatcaacct ggtgaccacg
     1441 gcaaagctgc tcgacgcgat cgaagcgcag atggagccgg ggctggccgt cgtgctgatc
     1501 gcctcgatgg ggcggctggt ttcgccgccc gccgagggcg ccctgggcga agtgctggag
     1561 aacccgctgg cgcccgattt cctcgaccgc atggagccgt tcctgggcga tgaggcgcag
     1621 aagccctatt ccgcctattc catcaccaag atgtgggtgg cacgcgaagt aaaccgccgg
     1681 gccatcagct gggggccgcg cggtgcgcgg atcatgtcga tctcgccggg gatgatctat
     1741 acccgcatgg gcacgagcgc cgtggccgaa gcgggggccg acgtgctgct ggagcagacg
     1801 ccggtgcgcc gctggggcac gcccatggac atcgccaacg tggccgagtt catcctctcg
     1861 gacaaggcga gcttcctcac cgggtcggac atcctggtgg acgggggcac cggccagctc
     1921 atccaggcgc tgaagggctg acgggcgcgg aggcgccggt ggtgctcagg ccccggcgtc
     1981 ttccagcacg gcggcgcgca gggcgtcgat ccccagcttc acttccgcgg aggtgaccag
     2041 cacctgcggg tgggccgcga catgcttgcg cgcctcggcc tcggtgccgg cggtgacttc
     2101 ggcgagttcg ctggccttca gcttgtcggc cttggtgagg acgacgcggt agccgaccgc
     2161 gctctcatcc agcatcttca tgaactcggc atcgaccggc ttgatgccgt ggcgggcatc
     2221 gaccagcagc agggtgcgct tgagcacgac gcggccgcgc agatagtcac ggatcaggcg
     2281 gcgccacttc tcgaccaccg cgggcggagc cttggcgaag ccgtagccgg gcatgtcgac
     2341 caggcggaac tgggtgggcg tgcccacctc gaagtagttc agctcctgcg tgcggccggg
     2401 cgtgaccgag gcgcgggcga tggccttgcg cccggtgagc gcattgagca gcgaggactt
     2461 gccgacgttc gaccggccgc agaaggcaat ttccggcacg gtgggatcgg gcaggaactt
     2521 gagctgcggg gccgacagca ggaattcgac gcggccggaa aacagctttc cggcacgctc
     2581 gatccgctcg gcgaggaggg tttcttcttc gctcaccctg gcgtcacttg gcccgcttgg
     2641 ccgcggcgcc gacatcggcc cgctccttct cgaccatggc cctgagctgg gggtgccgcg
     2701 cgtagaggta cttctgctgg gcgatggtga gcaggttcga ggtgatccag tagatcagga
     2761 ggcctgccgc aaacggcgcc atcacgaaca tcatcatcca gggcatgatc gagaagatct
     2821 gcttctgcat atcgtccatc ggcgccggat tgagcttgaa ctggaagaac atggtggtgc
     2881 ccaggatcac cgccagcacg ccgatgccca ggaaggccgg gggcgtgaac ggcaacaggc
     2941 cgaacaggtt gaggatgtgc agcggatcgg gcgcggacag atccttgatc cacaggatga
     3001 agggctggtg
//
LOCUS       DATNBV010000112         7261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4217298,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000112 DATNBV010000000
VERSION     DATNBV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4217298"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VN627_02720"
     CDS             <1..219
                     /locus_tag="VN627_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXD02199.1"
                     /translation="LGIHYNDADHDLLTYVADPAPFAVSDGTVPVPEGPGLGVEIDEA
                     KVREAAGRMTKGWRNPVWRGPDGAVREW"
     gene            complement(383..1720)
                     /locus_tag="VN627_02725"
     CDS             complement(383..1720)
                     /locus_tag="VN627_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HXD02200.1"
                     /translation="MQSAPPFNPFFMFATGIDSAAPMIDGIRHDQMELTGHYERWDSD
                     IDLVKDMGTRYLRFGPPVHRAYQAHGRYDWAHADAVLGRLYDYNIIPIADLCRLGVPD
                     WIGDFQNPDFPRHFAAYARAFAKRYPWIQLYAPVSTIFVTAFTSGKMGLLNEARSDDA
                     SFVTALKHLCAACVMAMIEILDVRPDAIFIQSERAAYFHADSPEAIGQAETLNAMRFL
                     ALDLIYGHRVESGMYEYLLDNGMTRTEYRWFMETRLGRHCVLGTDYYPANERRVDAQG
                     VRHPAGETLGYDDIVVQYHQRYRLPVMHTETSVPQGLDGEASVNWLWKEWANALRVRN
                     NGVPLVGFTWFPLIDQIGWSESGRIAPGDLRDRINPVGLYDVNRQRRPVGDAYRQLIR
                     DWRHLLPAQSVCLLMPIVLPSEYEEPMARHRRELIHRYYARRPEDMPANPFAG"
     gene            complement(1741..2625)
                     /locus_tag="VN627_02730"
     CDS             complement(1741..2625)
                     /locus_tag="VN627_02730"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXD02201.1"
                     /translation="MAIMTAPAVRAVQRGEASLGEGPVWVASEGALYWLDIHGRRILA
                     YRPDDGSCRQWHCSLRITAIAPCTSGGFIAGTERGFIRLTGLPGDCIRLGNPEPDLPG
                     NRGNDGKTDPFGHFWLGTMDDAEEQRSGALYRLDPDLAWHRMADGYGVPNGPAFSPDG
                     RWLYHTDSAERTIWRFALSADGSLADRQVFARFDERHGYPDGMTTDAEGHLWVAFWDG
                     WCLRRFAPSGEQVDELPLPVQRPTSCAFGGAALDRLFVTSARVGLSPEALAAQPDAGS
                     LFACEVAVPGLAPTLFPA"
     gene            2758..3654
                     /locus_tag="VN627_02735"
     CDS             2758..3654
                     /locus_tag="VN627_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HXD02202.1"
                     /translation="MHEWNDGFIAVDWGTTNRRAYRIDPAGAVVAEMEDESGVTAIAP
                     EGFPVALGQIVHRLGDLPLLMAGMIGSNRGWREAPYVRCPASVADLARNLLWIEPGRV
                     AIVPGVAWSGPGRADVMRGEEVQVFGHLAQARADEAWLCHPGTHTKWIKVADGAIEQF
                     RTLMTGELFALLRDHSILAPLLHADVEPGADFLAGVERGLEGADLAAELFSVRAGVLL
                     GQRDAATAAGWVSGLLVGADVRGGLASWGVGRPRCVTVLGRPTLNRLYCAALAHAGVL
                     AVGADGGPAFVAGMQSIRKALS"
     gene            3651..4310
                     /locus_tag="VN627_02740"
     CDS             3651..4310
                     /locus_tag="VN627_02740"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HXD02203.1"
                     /translation="MNGRTCHERFKANLAACPLVAIIRGVRPDETEAVGDALLEAGIR
                     VIEVPLNSPDPLRSIERLVRHAAGGATVGAGTVLDPDDVSRVAGVGGELIVAPNTDPA
                     VIAATVAAGLVSAPGFYTPSEAFIAVRAGAHALKLFPAEAASPAVLKAQMAVLPKDRP
                     VLVVGGVRPDTMQSWLEAGATGFGLGSGLYTPGLSPDEVGRRARDYVAGLGTSPLPNL
                     H"
     gene            complement(4336..5208)
                     /locus_tag="VN627_02745"
     CDS             complement(4336..5208)
                     /locus_tag="VN627_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HXD02204.1"
                     /translation="MRIVLAAGALEAHVLPAAGGSIARLDRVETGARRPLLRGTDDDR
                     ARALDCGCFPLVPFCNRIRGGAFSFAGRSVRLTPNMPPDPSPIHGQGWQGEWQVVADS
                     PAAVELRFDHDPGEWPWAYQARQVIALDPAGLSIELACTNLSDEPMPCGLGLHPYYPC
                     DSTTRLDTRVTGAWTVDADVLPVAHVPASGRYDLTDRAICGQDLDNGFDGWSGVATIR
                     WGDGMGLHLTSPDARFFQVFSPKEGGLFVAEPVSHANCALNAPEADWAQLGIVVLPPG
                     SEQVLRARLEVVVT"
     gene            complement(5205..5936)
                     /locus_tag="VN627_02750"
     CDS             complement(5205..5936)
                     /locus_tag="VN627_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD02205.1"
                     /translation="MAGRLEGKVAIVTGAAGGIGAATVRRLAADGARVAIADLDQGRA
                     AELAKSIGDQALPLVCNVADETAVAACVAATVEHFGRLDVVVNNAGLMTFKSLGDWTA
                     DDWHKVLAVDLVGAAMFVREAFRHMTGGGAVVNVASVHAERTTASCAPYSAAKAAMLS
                     LTRTAAIEGKARQIRANAVLPGAVDTPMLWENPNLKSGAEKIERADVGQPEDIAAAIA
                     FLASDDARFITGAALAVDGGRLAQL"
     gene            complement(5936..>7261)
                     /locus_tag="VN627_02755"
     CDS             complement(5936..>7261)
                     /locus_tag="VN627_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HXD02206.1"
                     /translation="PIGRRTIATFMLSTGIENSVPTIDNGRTRVDEMESCGHYRLWRE
                     DFRLCCEMGIGYLRYGPPIHTTWLAPGKYDWEFADITFAELKRLGIVPIVDLCHFGVP
                     NWLGNFQNGDFPALFEVYARDFAKRFPWVQLYTPVNEMFICAMFSAQYGWWNEQLSSD
                     TAFVTALKHIVKANVLAMRAILEVRPDAIFIQSESSEYFHAENPAAIRPAELLNSKRF
                     LSLDLNYGRRVDSEMYEYLMDGGMTRAEYHFFLDNRLKQHCIMGNDYYKTNEHRVAAD
                     GMTCASGEVFGYDEITRQYHARYGLPVMHTETNLNEGPLGTESADWLWKQWANVLRVR
                     NDGVPIVGFTWYSLTDQVDWDSALRFRNGNVNPLGLFDLDRNIRKVGEEYRKLIAAWR
                     DVLPTQSICLTVPVVPIAEIGTVHEANQADRARDIFVGASSSPSQSENG"
BASE COUNT         1174 a         2303 c         2612 g         1172 t
ORIGIN      
        1 ctcggcatcc actataacga tgccgaccat gatctgctga cctatgtggc cgacccggcg
       61 ccgtttgccg tgagcgacgg cacggtgccc gtgccggagg ggcctggcct cggggtggag
      121 atcgacgagg ccaaggtccg cgaggccgcc gggcgcatga ccaagggctg gcgcaatccg
      181 gtgtggcgcg ggccggatgg ggcggtgcgc gagtggtgag gggctagtca cgtgatgcct
      241 tggcctgccc gtcatcctga aactgtttca ggatccatgg cgcaaggatg gcgcactgtc
      301 gaacatggcg gacttggcgc gaccgtcggt ccatggatcc tgaagcaagt tcaggatgac
      361 ggtccaggcg agggtgggcc agtcaccccg cgaacggatt ggccggcatg tcttccggcc
      421 gccgcgcgta gtagcggtgg atcagttcgc ggcggtggcg ggccatcggt tcctcgtatt
      481 cgctgggaag gacgatcggc atcagcaggc agaccgactg cgcgggcagg agatggcgcc
      541 agtcgcggat cagctgccgg taggcatcgc cgacggggcg ccgctggcgg ttcacgtcgt
      601 aaagtcccac cgggttgatc cggtcgcgca ggtcgccagg cgcgatgcgg ccgctctcgc
      661 tccagccgat ctggtcgatc agcggaaacc aggtgaagcc gacgagcggc acgccattgt
      721 tgcggaccct cagcgcattg gcccattcct tccacaacca gttgaccgac gcctccccgt
      781 caagcccctg cggcacgctg gtttcggtgt gcatgaccgg caggcggtag cgctggtggt
      841 actggacgac gatatcgtcg tagcccagcg tctcgccggc ggggtggcgc acgccctggg
      901 catcgacccg gcgttcgttg gcggggtagt aatcggtgcc cagcacgcaa tggcggccaa
      961 gccgcgtttc catgaaccag cgatattcgg tgcgggtcat cccgttgtcg agcaggtact
     1021 catacatgcc cgattccacc cgatgcccgt agatcaggtc gagcgcgagg aagcgcatgg
     1081 cgttcagtgt ctcggcctgg ccgatggcct cgggcgaatc cgcgtgaaaa taggcggcgc
     1141 gctcgctctg gatgaagatc gcatcggggc gaacgtcgag gatctcgatc atagccatca
     1201 cgcaggccgc gcacaggtgc ttgagcgcgg tgacgaagct ggcgtcatcg cttctcgcct
     1261 cgttgagcaa gcccatcttg cccgaggtga aggcggtaac gaatatcgtg ctcaccggcg
     1321 cgtaaagctg gatccacgga tagcgcttgg caaaggcgcg ggcataggcg gcgaagtggc
     1381 gggggaaatc ggggttctgg aaatcgccga tccagtcggg cacgcccagc cggcagagat
     1441 cggcgatggg tatgatattg tagtcataga gccggccgag cacggcatcg gcgtgggccc
     1501 agtcgtagcg gccatgggcc tggtaggcgc ggtgcaccgg ggggccgaag cgcaggtagc
     1561 gcgtgcccat gtccttcacc aggtcgatgt cgctgtccca ccgctcgtag tggccggtca
     1621 gttccatctg atcgtggcga atgccgtcga tcatgggggc ggcggaatcg atgccggtgg
     1681 cgaacatgaa gaaaggattg aacggaggag cagactgcac gcgcgatctc tttcgatggg
     1741 tcaggcggga aacagggttg gggcaaggcc gggcacggca acctcgcagg caaacaggct
     1801 gccggcgtcg ggctgggcgg ccagcgcctc gggcgagagg ccgacccggg ccgaggtgac
     1861 gaacagccgg tccagcgctg ccccgccgaa agcgcaggag gtggggcgct ggacgggaag
     1921 gggcagttcg tcgacctgct cgcccgaggg agcgaagcgg cgcaagcacc agccgtccca
     1981 gaaagcaacc cagaggtgcc cctcggcatc ggtggtcatc ccgtcgggat agccatggcg
     2041 ttcgtcgaac cgcgcgaaga cctgccggtc ggccaggctg ccgtcggcgg acagggcaaa
     2101 gcgccagatc gtccgctcgg cggaatcggt gtggtagagc cagcgtccat ccgggctgaa
     2161 ggccgggccg ttgggcaccc cgtagccatc ggccatgcgg tgccaggcga ggtcggggtc
     2221 gagccggtaa agcgcgccgc tgcgctgctc ttcggcatcg tccatcgtgc cgagccagaa
     2281 atggccgaac ggatcggtct tgccgtcgtt gccgcgattg ccgggcaggt ccggttcggg
     2341 attgcctagc cggatgcaat cgccgggaag gcccgtcagc ctgatgaagc cgcgctcggt
     2401 cccggcgatg aagccgccgc tggtgcaggg cgcgatggcg gtgatgcgca gcgaacagtg
     2461 ccactggcgg caggagccgt catccgggcg gtaggcaagg atgcgccggc cgtggatgtc
     2521 gagccagtag agcgcgccct cgctggcgac ccagacaggt ccttcgccga ggctcgcctc
     2581 tccccgctgc acggcacgca ccgccggggc cgtcatgata gccatcacgc ttcactccca
     2641 atcaccggcc tcgcaagggt gagtagaggg aacctgccgg ggaggagccg gttccatcgg
     2701 ggcagagccc tgctgccggc gcgcagcctt gccccccgca tttacgagga cccagcgatg
     2761 cacgagtgga acgacgggtt catcgccgtg gactggggca cgaccaaccg ccgcgcctac
     2821 cgcatcgatc cggcaggcgc ggtcgtcgcc gagatggaag acgaatccgg ggtgacggcg
     2881 atcgcgcccg aaggcttccc ggtcgccctg gggcagatcg tccaccggct cggcgatctg
     2941 ccgctgctga tggcgggcat gatcggctcc aaccggggtt ggcgcgaggc gccctatgtg
     3001 cgctgcccgg caagcgtggc ggacctggcg cgcaatctgc tctggattga gccgggccgg
     3061 gtggccatcg tgcccggggt cgcctggtcg gggccgggcc gggccgatgt gatgcgcggc
     3121 gaggaggttc aggtcttcgg ccatctggcc caggcgcgtg cggatgaggc atggctgtgc
     3181 catcccggca cccacaccaa gtggatcaag gtggcggacg gcgcgatcga gcagttccgt
     3241 acgctgatga caggagagct gttcgccctg ctgcgcgacc attccatcct cgcgcccctg
     3301 ttgcatgccg atgtggagcc cggggcggac ttcctcgccg gggtcgaacg cggtctggaa
     3361 ggcgccgacc tggccgccga gctgttttcc gttcgcgccg gggtgctgct gggccagcgc
     3421 gatgcggcga cggcggccgg atgggtcagc ggcctgctgg tcggggccga cgtgcgcggc
     3481 gggctggcca gctggggcgt gggccgaccg cgctgcgtga ccgtgctcgg ccgccccacg
     3541 ctgaaccggc tctactgcgc cgcactggcc catgccgggg tgctggcagt tggcgcggac
     3601 ggcgggccgg ccttcgtggc gggcatgcag tccatcagaa aggcgctctc atgaatggcc
     3661 gaacctgtca cgaacgcttc aaggccaacc tcgccgcctg cccgctggtg gcgatcatcc
     3721 gcggggttcg gcccgacgag acggaggcgg tgggtgatgc cttgcttgag gccggaatcc
     3781 gggtgatcga agtgccgctc aattcgcccg atcccctgcg cagcatcgag cggctggtcc
     3841 gccacgcggc gggcggcgcg actgttggcg cggggaccgt gctggacccg gacgacgtgt
     3901 cgcgcgtcgc gggtgtcggc ggggagctga tcgtggcgcc caataccgat ccggcggtga
     3961 tcgccgcaac ggtcgctgcc gggctggttt cggcgccggg gttctatacg ccgagcgagg
     4021 ccttcatcgc ggttcgcgcc ggggctcatg cgctcaagct gtttccggcc gaggcggcga
     4081 gcccggcggt gctcaaggcg cagatggccg ttctgccgaa ggaccggccg gtgctggtgg
     4141 tcggcggggt tcggccagac accatgcaga gctggctgga ggccggggcg accggattcg
     4201 ggctcggctc ggggctgtat acgccgggtc tttcgccgga tgaagtcggg cggcgggcgc
     4261 gggattatgt ggcggggctg gggacatcac ccctgcccaa cctccactag ggggctggat
     4321 ggggtgagcc cgtggctaag tgacgaccac ctccaaccgc gcccgcagaa cctgctcact
     4381 gccgggaggc agtacgacga tccccagctg cgcccaatcc gcctccggcg cattcagcgc
     4441 gcagttggca tggctgaccg gttccgccac gaacaggccg ccctccttcg gggaaaatac
     4501 ctggaagaag cgggcatcgg gggaagtcag gtgcaggccc atcccgtccc cccagcggat
     4561 cgtcgccacg ccgctccagc catcgaagcc attgtcgagg tcctgcccgc agatcgcccg
     4621 gtcggtgaga tcgtaccggc cacttgccgg gacatgggcc acggggagga catcggcgtc
     4681 gactgtccac gctccggtga ccctggtgtc cagccgggtt gtgctgtcgc acggataata
     4741 ggggtgcagg ccaaggccgc agggcatggg ttcgtcggac aagttggtgc aggcaagctc
     4801 gatcgatagc ccggcgggat cgagcgcgat gacctgccgg gcctggtaag cccagggcca
     4861 ttctcccgga tcgtgatcga agcgcagttc gacggctgcg gggctgtcag ccacgacctg
     4921 ccactcgccc tgccaaccct gaccgtggat gggggagggg tcgggcggca tgttgggggt
     4981 gaggcggaca gagcgcccgg cgaagctgaa tgcgccgccc cggatgcggt tgcagaacgg
     5041 caccagggga aagcagccgc aatccagcgc gcgcgcccgg tcgtcgtccg tgccgcgcag
     5101 gagcgggcgg cgggcaccgg tttcgacccg gtccagccgg gcgatgctgc cgccggcggc
     5161 gggaagtaca tgggcctcca atgccccggc ggcgaggact attctcatag ttgcgccagc
     5221 cggcccccgt cgaccgccag cgccgcgccg gtaatgaacc gggcatcgtc gctggcaagg
     5281 aaggcgatgg ccgcggcgat gtcctcgggc tggccgacat ccgcgcgctc gattttttcg
     5341 gcgccggact tgagattggg attctcccag agcatcggcg tatccaccgc cccggggagc
     5401 acggcattgg cgcggatctg gcgcgccttg ccctcgatcg ctgccgtgcg ggtgagcgag
     5461 agcatggccg ccttggccgc ggaatagggc gcgcaggagg cggtggtccg ctcggcatgg
     5521 acgctggcga cgttcaccac cgccccgcct cccgtcatgt gccgaaaggc ctcgcgcacg
     5581 aacatcgccg cgccgaccag atcgaccgcg aggaccttgt gccaatcgtc ggcagtccaa
     5641 tcgccaagcg acttgaacgt catcagcccg gcgttgttga ccaccacgtc gagccgcccg
     5701 aaatgttcga ccgtggccgc gacgcaggcg gcgacggcgg tttcatcggc gacattgcag
     5761 acgagcggca gggcctggtc gccgattgat ttggcgagtt cggcggcacg gccttggtcg
     5821 agatcggcaa tggcgacccg ggcgccgtcc gcagccagcc gccgcaccgt cgccgcgccg
     5881 atcccgcctg ccgcgccggt gacgatcgcc accttgccct cgagccggcc cgccatcacc
     5941 cgttctcgct ctggctgggc gagctcgacg cgccgacgaa gatatcgcgc gcgcggtcgg
     6001 cctggttggc ctcgtggacc gtgccgatct cggcgatggg gacgaccggc acagtgaggc
     6061 agatcgactg ggtcggcaaa acatcgcgcc aggcggcgat cagcttgcgg tattcctcgc
     6121 ccactttccg gatattgcga tcgaggtcga acaggccaag cggattgacg ttgccgttgc
     6181 ggaagcgcag cgcgctgtcc cagtcgacct gatcggtcag cgaataccag gtgaagccga
     6241 cgatcggcac cccgtcgttg cgcacgcgca ggacattggc ccactgcttc cacagccagt
     6301 cggcgctttc ggtaccgagc ggcccctcgt tgaggttggt ctcggtgtgc atgaccggga
     6361 ggccgtaacg ggcgtgatac tggcgggtga tctcgtcata gccgaagacc tcgcccgatg
     6421 cgcaggtcat gccgtccgcc gccacgcggt gctcgttggt cttgtagtag tcgttgccca
     6481 tgatgcagtg ctgcttgagc cggttgtcga ggaagaagtg gtattccgcg cgggtcatcc
     6541 cgccatccat caggtactcg tacatctcgg aatcgacccg ccggccatag ttcaggtcga
     6601 ggctgaggaa ccgcttggaa ttcaacagtt cagcgggacg aattgccgcc gggttctccg
     6661 catggaaata ctccgagctt tccgactgga tgaagatcgc gtcgggccga acctcgagaa
     6721 tggcgcgcat cgccagcacg ttggccttca cgatgtgctt gagcgcggtg acgaaggccg
     6781 tgtcgctgga aagctgctca ttccaccagc cgtattgcgc cgagaacatc gcgcagatga
     6841 acatctcgtt gaccggcgta tagagctgga cccagggaaa gcgcttggcg aaatcccggg
     6901 cgtaaacctc gaacagggca ggaaaatcgc cgttctggaa attgcccagc cagttgggca
     6961 cgccgaagtg gcacagatcg acgatcggaa cgatgccgag ccgcttcagc tcggcgaagg
     7021 tgatgtcggc gaattcccag tcatacttgc cgggggccag ccaggtcgtg tggatcggcg
     7081 ggccatagcg caggtagccg atgcccatct cacagcacag gcggaagtcc tcgcgccaca
     7141 atcggtagtg gccgcaggac tccatctcgt ccacccgggt gcggccattg tcgattgtgg
     7201 gaacggagtt ctcgatcccg gtggaaagca tgaacgtggc gatcgttcgt ctcccgatcg
     7261 g
//
LOCUS       DATNBV010000113         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_381286,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000113 DATNBV010000000
VERSION     DATNBV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381286"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VN627_02760"
     CDS             complement(<1..286)
                     /locus_tag="VN627_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXD02207.1"
                     /translation="MKVYYDADCDLNLVTGKKIAILGYGSQGHAHAQNLRDSGVKEVA
                     IALRPGSATAKKAEGAGFKVLSNTQAAEWADIVMILAPDEHQAAIWENDLK"
     gene            complement(399..914)
                     /gene="ilvN"
                     /locus_tag="VN627_02765"
     CDS             complement(399..914)
                     /gene="ilvN"
                     /locus_tag="VN627_02765"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HXD02208.1"
                     /translation="MKITKEAEERHVLTVIVDNEAGILAKIAGLFTARGYNIDSLTVA
                     DITENHAVSRITIVTHGPPAQIDQIHAQLERLVPVHKVIDLTENGPHVERELALVKVV
                     GTGDARVEAMRLADIFRARPVDTTTSSFIFELTGAPDKIDTFIGLMRELGLVEVGRTG
                     VVGMARGTQGV"
     gene            complement(1000..>2549)
                     /gene="ilvB"
                     /locus_tag="VN627_02770"
     CDS             complement(1000..>2549)
                     /gene="ilvB"
                     /locus_tag="VN627_02770"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HXD02209.1"
                     /translation="TGKPGCVLVTSGPGATNAVTGIADAFMDSIPMVVITGQVPTSVI
                     GSDAFQEADTVGITRHCCKHNYLVKDPADLAAVINEAFQIATTGRPGPVVVDIPKNVQ
                     IATAAWSEAAPQRRNRYSPRTRGTDDEIARAVELLANAKQPVLYTGGGVVNSGPRASQ
                     LLRELQQLTGAPLTSTLMGLGSFPADHADWLGMLGMHGTYEANMAMNKADLILCVGAR
                     FDDRVTGRLDAFSPDSTKIHIDIDRSSINKTVPVDLPIVGDCAEVLEQIITGWGNRKP
                     QDLTEWKARIDGWRARNSLAYPRTPAAIAPQLAVERLFALTREHDPIITTEVGQHQMW
                     AAQHFGFFAPNRWLTSGSLGTMGYGLPAAIGAQMGNPGKLVIDIAGDASIQMNIQEMG
                     TATQYRLPVKVFVLNNEFMGMVRQWQELTYESRYSNSYSDSLPDFVKLAEAYGWKGIR
                     IESEDQLDAGIKAMIEHPGPVFVDCLVAKEDNCFPMIPSGAAHTEMLLYGDTVEGTMD
                     DEAKALV"
BASE COUNT          402 a          815 c          840 g          492 t
ORIGIN      
        1 ccttgaggtc gttctcccag atcgccgcct gatgttcatc gggcgcgagg atcatgacga
       61 tatcggccca ctcggccgcc tgggtgttgg acaggacctt gaacccggcg ccttccgcct
      121 tcttcgcggt cgccgaaccc ggccgcagcg cgatcgcgac ttccttcacg ccgctgtcgc
      181 gcaggttctg cgcatgggca tggccctgcg agccatagcc gaggatggcg atcttcttgc
      241 cggtgaccag gttcaggtcg caatcggcgt cgtagtaaac cttcatgttt ccgctctttc
      301 cttgcctgtc cgcctccggg cgcaagcccc gggaagctgg accccggagc aggtccgggg
      361 tgacgataaa cgtgagaatc cgataaaact ggcgcccctt agacgccttg cgtcccgcgc
      421 gccataccca cgacgccggt gcggcccact tcgaccagcc ccagctcgcg catcaggccg
      481 atgaaggtgt cgatcttgtc cggtgcgccg gtcagctcga agatgaagct cgaggtcgtg
      541 gtgtcgaccg gcctggcgcg gaagatgtcg gcaaggcgca tggcctcgac ccgcgcatcg
      601 ccggtgccga ccactttcac cagcgccagt tcgcgctcca cgtgcgggcc gttttcggtg
      661 aggtcgatta ccttgtggac cggcaccagc cgctccagct gggcgtgaat ctggtcgatc
      721 tgcgccgggg ggccatgcgt cacgatggtg atccggctca ccgcgtggtt ctcggtgatg
      781 tcggccacgg tgaggctgtc gatattgtag ccgcgcgcgg tgaacaggcc cgcgatcttg
      841 gcgagaatcc cggcctcgtt gtcgacgatg acggtgagga cgtggcgttc ctcggcttcc
      901 ttggtaatct tcatcatcaa atcctacccg ccgcaccgat gcgcgcattg ctgaaatcct
      961 ccccacgtgt ggggaggatt tggatgttcg ccgccagggt cacaccagcg ccttcgcttc
     1021 gtcgtccatc gtgccctcga ccgtatcgcc gtagagcagc atctcggtat gcgccgcgcc
     1081 cgaggggatc atcgggaagc agttgtcttc cttcgcgacg aggcagtcga cgaagaccgg
     1141 gccgggatgc tcgatcatcg ccttgatccc ggcgtccagc tggtcctcgc tttcgatgcg
     1201 gatgcccttc cagccatagg cctcggcgag cttcacgaaa tcgggcaggc tgtctgaata
     1261 ggaattcgag tagcggctct cataggtcag ttcctgccac tggcggacca tgcccatgaa
     1321 ttcgttgttg agcacgaaaa ccttgaccgg caggcgatat tgcgtggccg tgcccatctc
     1381 ctggatgttc atctggatcg aggcatcgcc cgcgatgtcg atgacaagct tgccggggtt
     1441 gcccatctgc gcgccgatgg cggcgggcag gccatagccc atggtgccca ggctccccga
     1501 ggtgagccag cggttcgggg cgaagaagcc gaagtgctgg gcggcccaca tctggtgctg
     1561 gccaacctcg gtggtgatga tcggatcatg ttcgcgggtg agggcgaaca gccgctcgac
     1621 cgccagctgc ggcgcaatgg ccgcgggggt gcgtggatag gccaggctgt tgcgcgcgcg
     1681 ccagccgtcg atccgggcct tccactcggt caggtcctgc ggcttgcggt tgccccatcc
     1741 ggtgatgatc tgctccagca cctcggcgca gtcgccgacg atgggcaggt cgaccggcac
     1801 cgtcttgttg atcgacgagc ggtcgatatc gatgtggatc ttggtcgaat ccggcgagaa
     1861 ggcatcgagc cgcccggtta cccggtcgtc gaagcgcgcg ccgacgcaaa ggatcagatc
     1921 ggccttgttc atcgccatgt tggcttcata ggtcccgtgc atgccaagca tgccgagcca
     1981 gtcggcatga tcagccggga atgacccaag gcccatcagg gtagaggtga gcggcgcgcc
     2041 cgtcagctgc tgcagctcgc gcaatagctg gctggcgcgc gggccggaat tgaccacgcc
     2101 gccaccggtg taaagcaccg gctgcttcgc attggccagc agttcgaccg cgcgggcgat
     2161 ctcgtcatcc gtgccccggg tgcgcgggct gtagcggttg cggcgctgcg gggcagcttc
     2221 gctccaggcc gccgtggcga tctggacatt cttgggaatg tcgacgacca ccggccccgg
     2281 gcgcccggtc gtggcgatct ggaatgcctc gttgatcacc gcggcaagat cggccggatc
     2341 cttgaccagg tagttgtgct tgcagcagtg ccgcgtgatg ccgacggtgt cggcttcctg
     2401 gaaggcgtcc gatccgatca cgctcgtcgg aacctgcccg gtgatgacga ccatcggaat
     2461 cgaatccatg aaggcgtcgg caatgccggt aacggcattg gtcgcccctg gccccgaggt
     2521 aacgaggacg cagccgggct tgcccgtcg
//
LOCUS       DATNBV010000114         5374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3257938,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000114 DATNBV010000000
VERSION     DATNBV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3257938"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VN627_02775"
     CDS             <1..725
                     /locus_tag="VN627_02775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD02210.1"
                     /translation="REPPRLEHSGRASASGFFGFVRANRGVIGVVFLCYGLISFAGYG
                     AIAWMATGYVRQFGLSLARAGVVVGAITLAASIVGAIVGGLLTDRWHARGVAGGRFLM
                     VVPSGIVGALAFAAWWSANSFGWSVAGGIVAFSMQVAAATTAPSVVAALAPNEYRGQL
                     AAFYLLVTGLFGIAAGPTAIALITDHVFADDAALRWSIIWATVPALLLAALVAWTSRG
                     YYSREAGKWQVPMNGMPEGRAG"
     gene            complement(729..2381)
                     /locus_tag="VN627_02780"
     CDS             complement(729..2381)
                     /locus_tag="VN627_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXD02211.1"
                     /translation="MGTGSEFDLIVVGSGAASVVAALVAVEAGARPVILEKTDKVGGS
                     TAMSGGVAWIPCNSLQREAGVADSYDQARAYLDACAGDAGPASSPERRHAFLTEGPQM
                     IDFLRGKGMAFVHAEGYSDYHEGQKPGALARSRSLVGQIYDARRLGAWQGRLRRNAGG
                     PPMLMHEAHDLGQNGRTWASRAAFVRVALRMLRNRLGADLVGNGMAIQGRLLEIALAA
                     GIEIRPETGVTGFVVEAGRVVGVTTQGPGGAGELRAARGVLINAGGFSHNLAMRQKYQ
                     QAPTGTDWTNANPGDTGEVMEAAMALGAAVDIMEQCWWVSISVTPDGTKLIHPFDMSK
                     PYSILVDQGGERFVNESTSYMEVGIRTYARDKVVKAVPSWFISDSRHRQRYRWAGLPT
                     GEPPREWLDAGYVVRADSIAELAERCGIPPAALTRTVERFNAFAERGVDDDFHRGEGA
                     YDRFGGDELVKPNPNLGAIARPPFYAIRFFPGDVGTCGGLMTDDKARVLRDDGSVIPG
                     LYATGNSTASVVGRSYPGAGASIGASMTFGYIAARDALGVNA"
     gene            complement(2607..2792)
                     /locus_tag="VN627_02785"
     CDS             complement(2607..2792)
                     /locus_tag="VN627_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HXD02212.1"
                     /translation="MSDQPETVYVTTHRVKCDGATDVRGGAALGHPRVWLEIDEKGYV
                     DCGYCDKRFVLKGEHAH"
     gene            2867..3811
                     /locus_tag="VN627_02790"
     CDS             2867..3811
                     /locus_tag="VN627_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXD02213.1"
                     /translation="MHEAAISIRNLVKRYAGAKGAPGKLALDDVSFDVPRGTIFGLLG
                     PNGAGKSTLINTLAGLVTKTSGEVSIWGFDIDHDTRNAKRAIGIVPQEIVFDPFFTPA
                     EVLEIQAGYYGIPKAARRTLELLAAVHLSDKAKAYSRTLSGGMKRRLLIAKAMVHSPP
                     VLVLDEPTAGVDVELRRQLWELVGELNGQGVTVVLTTHYLEEAEELCDRIAIINHGKL
                     IANKSTADLLSTAREKIVVLTLDRDLPAELPSHPAFLKCEVAGERTIEITYDKDRTNA
                     GEVLALVQGQGFVIADVTTREADLEDVFVALTSADRTA"
     gene            3826..>5374
                     /gene="nadB"
                     /locus_tag="VN627_02795"
     CDS             3826..>5374
                     /gene="nadB"
                     /locus_tag="VN627_02795"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HXD02214.1"
                     /translation="MTHDVIVIGSGGAGLTAALALAEKLKVLVLAKGTLTSGSTAWAQ
                     GGIAAVLDAGDTFENHVRDTMVAGAGLNRRETVEFVISQAPLAIDRLVELGVPFNTAK
                     NEGGLENLHLTREGGHSHRRIVHVDDATGWAVQAALLKAAEANPNITLLAGRTCIDLI
                     TGRHEERYSGSGRVWGVYALDEATGKVEAHTARATVLATGGAGRVYQFSTAPRGATGD
                     GIAMAWRAGARVGNMEMMQFHPTCLYNLEVKNFLITEAVRGEGGRLINPRTHKRFMEF
                     YDAERMELAPRDVVARAIDSEIKKYGLDHVHLDISHMPADFVREHFPTIHEKLLTLGI
                     DMTRQPIPVVPAQHYTCGGVMIDLDGRTDLPGLYAAGECTESGLHGANRLASNSLLEC
                     FVFGEAAARHILAHWDSFDAPPPVRAWDESRVTNSDEEVVIKQNWTEIRRFMWNYVGI
                     VRTTKRLERAAHRIKLLTDEVGDYYGHFRVTTDLIELRNLLQSADLIVKSALRRHESR
                     GLHYTLDY"
BASE COUNT          841 a         1812 c         1846 g          875 t
ORIGIN      
        1 tgcgcgagcc gccgcggctc gaacattccg gccgtgcttc ggcctccggc ttcttcgggt
       61 ttgtgcgggc caaccgcggt gtaatcgggg tagttttcct ctgctatggc ctcatcagct
      121 tcgccggcta cggggcgata gcctggatgg cgacgggcta tgtgcgccag ttcggcctgt
      181 cactggcgag ggcgggggtc gtggtcggcg cgatcacgct ggccgcctcg attgttggcg
      241 ccattgtcgg cggcctgctg accgacaggt ggcacgcgcg cggagtggct ggcgggcggt
      301 tcctgatggt cgttcccagc gggattgtcg gcgccctggc tttcgccgca tggtggagcg
      361 ccaattcatt cggctggtcc gtggcgggcg ggatcgtggc attttccatg caggttgcgg
      421 cggccacaac ggcgccctcc gtcgtcgccg cgctcgcgcc caacgagtat cgcgggcaac
      481 tggccgcgtt ttacctgctg gtgacgggcc tgttcggtat cgccgccgga ccaaccgcca
      541 tcgccctcat caccgatcac gtctttgccg acgacgccgc gctgcgctgg tcgatcatct
      601 gggcgaccgt gcccgccctg ctgctggccg ctctggttgc ctggacctcg cgcggctatt
      661 actcgcgcga ggcggggaaa tggcaggtcc cgatgaacgg catgccggag gggcgggctg
      721 gctgatcgtc aggcgttcac gcccagcgca tcgcgcgcgg cgatgtagcc gaaggtcatc
      781 gaggcaccga tactggcgcc tgcccccgga taggagcggc ccacgaccga ggcggtcgaa
      841 ttgccggttg cgtagagccc ggggatcacc gacccgtcat cgcgcagcac ccgcgccttg
      901 tcgtcggtca tcagcccccc gcaggtgccg acatcgcccg ggaagaagcg aatcgcgtag
      961 aacgggggtc tggcgatagc gccgaggttg gggttgggct tgaccagctc atcgccgccg
     1021 aaccggtcgt aggcgccttc gccgcgatgg aagtcgtcgt ctacgccccg ttcggcgaag
     1081 gcgttgaacc gctctaccgt ccgcgtcagc gcagcggggg gaatgccgca gcgctcggcc
     1141 agttcggcga tggaatcggc ccgcacgaca tagccggcgt cgagccattc acgcggcggt
     1201 tcgccggtcg gcaggccggc ccagcggtag cgctgccggt ggcggctgtc gctgatgaac
     1261 cagctcggca cggctttgac caccttgtcg cgcgcatagg tgcggatgcc cacctccatg
     1321 taggaggtgg attcgttgac gaaccgctcc ccgccctggt cgaccaggat cgagtagggc
     1381 ttcgacatgt cgaacggatg gatcagcttg gtcccgtccg gcgtgaccga gatcgagacc
     1441 caccagcact gctccatgat gtcgaccgcg gcgccgagcg ccatggccgc ttccatcacc
     1501 tccccggtgt cgccgggatt ggcgttggtc cagtcggtgc cggtcggcgc ctgctgatac
     1561 ttctgacgca tggcgaggtt gtgcgaaaat ccgccggcat tgatcagcac accgcgggct
     1621 gcgcgcaatt cgcctgcccc gccgggacct tgcgtcgtca cgcccacgac gcggccggcc
     1681 tcgaccacga aaccggtgac gcccgtctcg ggccggatct cgattccggc agccagcgcg
     1741 atttccagca gccggccctg gatggccatg ccgttgccga cgaggtccgc gccgagccgg
     1801 ttgcgcaaca tgcgcagggc gacacgcacg aaggccgccc ggctggccca ggttcgcccg
     1861 ttctggccca ggtcatgcgc ctcgtgcatc agcatcgggg ggccgccggc attgcgccgc
     1921 agccggccct gccaggcgcc gagccggcgc gcatcgtaga tctggcccac cagcgagcgg
     1981 ctgcgcgcga gagcgccggg cttctgccct tcgtgatagt cggaatagcc ttcggcatgg
     2041 acgaatgcca tgcccttgcc gcgcaggaaa tcgatcatct gcggcccttc cgtcaggaag
     2101 gcatggcgcc gctcggggga ggacgccgga ccggcatcgc cggcgcaggc atcgagatag
     2161 gcgcgggctt ggtcgtagct gtccgccacg cccgcttcgc gctggagcga attgcacgga
     2221 atccacgcga ccccgcccga catggcggtg gagccgccca ccttgtcggt tttctccagg
     2281 atcaccggcc gcgccccggc ttccaccgcc accagtgcgg cgaccaccga agctgccccg
     2341 ctgccgacca cgatcagatc aaattcgctg cccgttccca tcgccgacct ctccaacgca
     2401 gctcaactga cggcccggga tcttgtgatc tcgctgggtc gtcggggcat tcgagcatat
     2461 tgcggttggc atgccaatgt ttaggcgcat caccgcagtg aattcaccgg acggtgaagc
     2521 tgtcgggggg cgctgcccga caggcagtct cgccgggttg cgagtcgacg tcggcaggtt
     2581 tcgcggacgc gtcgcgacgg cgcggctcag tgtgcgtgct cgcccttcag gacgaagcgc
     2641 ttgtcgcagt agccgcaatc gacatagccc ttctcgtcga tctccagcca gacgcgcggg
     2701 tggccgagcg ccgcgccgcc gcgcacgtcg gtggcgccat cgcacttcac gcggtgggtg
     2761 gtgacgtaga cggtttctgg ctggtcgctc attgggcagt tttccgttgc tgggcgcttg
     2821 gctttgccct ttacgcgaag cccgcgctgc cgcaagagcc tgtgccatgc atgaggccgc
     2881 aatctcgatc cgcaatctgg tcaagcgcta tgccggggcg aagggcgcgc cgggcaagct
     2941 cgcgctcgac gatgtctcgt tcgacgtgcc gcgcggcacc atcttcggct tgctggggcc
     3001 gaacggggcg ggcaagtcga ccctgatcaa cacgctcgcg ggcctggtga cgaagacctc
     3061 gggcgaagtc tcgatctggg gcttcgatat cgaccacgat acccgcaacg ccaagcgcgc
     3121 gatcgggatc gtgccgcagg aaatcgtgtt cgacccgttc ttcacgcccg ccgaagtgct
     3181 ggagatccag gctggctatt atggcatccc caaggcggcg cggcgcacgc tcgagctgct
     3241 ggcggcggtg cacctgtcgg acaaggccaa ggcctattcg cgcacgctgt cgggcggcat
     3301 gaagcggcgc ctgctgatcg ccaaggcgat ggtccacagc ccgccggtgc tggtgctgga
     3361 cgagccgacc gcgggggttg acgtggaact gcggcggcag ttgtgggaac tcgtcggcga
     3421 gctcaacggg cagggggtga ccgtggtgct gaccacccat tacctcgagg aagccgagga
     3481 gctgtgcgac cggatcgcga tcatcaacca cggcaagctg atcgccaaca agtccaccgc
     3541 cgatctgctc tccaccgcgc gggaaaagat cgtggtgctc acgctggacc gagatctgcc
     3601 ggccgagctg ccgagccatc cggccttcct caagtgcgag gtcgcgggcg agcggaccat
     3661 cgagatcacc tatgacaagg atcgcaccaa cgcgggcgag gtgctggcgc tggtccaggg
     3721 gcagggcttc gtcatcgccg acgtgaccac ccgcgaggca gacctcgagg acgtgttcgt
     3781 ggcgctgacc agcgcggacc ggacggcctg agcgggaggc gcggcgtgac ccacgacgtt
     3841 atcgttatcg gctcgggcgg ggcggggctc accgcggcgc tggcgctggc cgagaagctc
     3901 aaggtgctgg tccttgccaa gggcacgctg accagcggct cgaccgcctg ggcccagggc
     3961 gggatcgccg ccgtgctcga tgcgggcgat accttcgaga accacgtgcg tgataccatg
     4021 gtggccggtg ccgggctcaa ccgccgcgag acggtggaat tcgtgatcag ccaggcgccg
     4081 ctggcgatcg accggctggt ggagcttggc gtgccgttca acaccgccaa gaacgagggc
     4141 gggctagaga acctccatct cacccgcgaa ggcggccatt cgcaccggcg gatcgtccac
     4201 gtcgacgacg ccaccggctg ggcggtccag gccgcgctgc tcaaggcggc ggaggccaat
     4261 cccaacatca ccctgctggc cgggcgcacc tgtatcgacc tcatcaccgg gcggcacgag
     4321 gagcgctatt cgggctcggg ccgcgtctgg ggagtctatg ccctcgatga ggccaccggc
     4381 aaggtcgagg cgcacacggc gcgggcgacg gtgctggcga ccggcggggc cggccgcgtc
     4441 taccagttct ccaccgcgcc gcgcggggcg accggcgacg gcatcgccat ggcctggcgc
     4501 gcgggggcgc gggtgggcaa catggagatg atgcagttcc acccgacctg cctctacaac
     4561 ctcgaggtca agaacttcct gatcaccgag gccgtgcgcg gcgagggcgg gcggctgatc
     4621 aacccgcgca cccacaagcg cttcatggaa ttctacgacg ccgagcggat ggaactggcg
     4681 ccgcgcgacg tggtggcccg cgcgatcgac agcgagatca agaagtacgg gctcgaccat
     4741 gtccacctcg acatcagcca catgccggcc gatttcgtgc gtgaacattt cccgacgatc
     4801 cacgaaaagc tgctgacgct gggcatcgac atgacccgcc agccgattcc cgtggtgccc
     4861 gcccagcact atacctgcgg cggggtgatg atcgacctcg acggccgcac cgacctcccc
     4921 gggctctatg ccgccggcga atgcaccgag agcgggcttc acggggccaa ccgcctcgcc
     4981 tccaactcgc tgctcgaatg cttcgtcttc ggcgaggcgg cggcgcggca tatccttgcc
     5041 cactgggaca gctttgacgc gcccccgccg gtccgcgcct gggacgagag ccgggtgacc
     5101 aattccgacg aagaggtggt gatcaagcag aactggaccg agatccgccg cttcatgtgg
     5161 aactacgtgg gcatcgtgcg cacgaccaag cggctggagc gagcggccca ccggatcaag
     5221 ctgctgaccg acgaggtggg cgactactac gggcacttcc gggtgacgac cgacctcatc
     5281 gaactgcgca acctgctgca gagcgcggac ctgatcgtga aatcggccct gcgccggcac
     5341 gagagccggg gcctgcacta cacgctggac tatc
//
LOCUS       DATNBV010000115         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3902517,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000115 DATNBV010000000
VERSION     DATNBV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3902517"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VN627_02800"
     CDS             <1..211
                     /locus_tag="VN627_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HXD02215.1"
                     /translation="GKKLKMLKRHLMTHYNLTPEQYRARWNLPADYPMVAPDYAEKRR
                     ELAKKIGLGRKPGAKPARGRAKASA"
     gene            357..800
                     /locus_tag="VN627_02805"
     CDS             357..800
                     /locus_tag="VN627_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HXD02216.1"
                     /translation="MHQPIDIEALCAERGLRITDQRRVIARVLSESEDHPDVDKLHER
                     AAAIDPRISIATVYRTVRLFEEAGILDRHDFGDGRARYEAAPEAHHDHMIDVETGRVI
                     EFVDPELEALQRQIAERLGYRLVDHRMELFGVRIGREKAEGGSGS"
     gene            805..1689
                     /locus_tag="VN627_02810"
     CDS             805..1689
                     /locus_tag="VN627_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXD02217.1"
                     /translation="MPAEASLDRPTPATRIGLAASLRIQGRLILILLTVLLGVPLHYV
                     CRALTRHSPIPRLTLRAIARIVGARVRIVGTPLRRDVFLLANHISWLDIPALAGASGT
                     AFVAKAEVAQVPVVGWLARLNRTVFVQREARLGVAHQINALREAMAENHALTIFPEGT
                     TTDGQSLLPFKTAMLKVLEPPPPGVMVQPVLLDYGRLAPFICWIGDEPGLANVIKVLA
                     RPGSFALTIRFLEPFSPEGHAGRKAIAAEARARIEAALVEALGKPLRTYGVTVDAVRY
                     APPPAPAVEAHDRAEGED"
     gene            1734..>2083
                     /locus_tag="VN627_02815"
     CDS             1734..>2083
                     /locus_tag="VN627_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXD02218.1"
                     /translation="MANPSPAPKTYRVKSFGCQMNVYDGERMAELLGSQGIAPAPEGE
                     EADLVVLNTCHIREKAAEKVYSDIGRIVKDWGRDDGSSPLIAVAGCVAQAEGEEIMAR
                     APAVKMVVGPQAYHR"
BASE COUNT          339 a          703 c          725 g          316 t
ORIGIN      
        1 cggcaagaag ctgaagatgc tcaagcgtca cctgatgacg cactacaacc tgacgcccga
       61 gcagtatcgc gcacggtgga atcttccggc cgactatccg atggtcgcgc ccgactatgc
      121 cgagaagcgc cgcgaactgg ccaagaagat cggcctcggc cgcaagcccg gcgccaagcc
      181 cgcccgcggc cgcgccaagg cttcagcctg atcggcaccc gggagcctcc ctgtggggag
      241 agccgccggg acagttgcga aatagccccg ccgggttcat atcgggcggg gctttttcgt
      301 gcgcggcgtc cggctgcgac gtgatctatc catgactggc cgacaacgga cgagacgtgc
      361 accaacccat cgacatcgag gcgctctgcg cggagcgtgg cctgaggatc accgaccagc
      421 ggcgggtgat cgctcgcgtg ctctcggaaa gcgaagacca tcccgacgtc gacaagctgc
      481 acgagcgcgc ggcggcgatc gacccgcgca tctccatcgc cacggtctac cgcacggtgc
      541 gcctgttcga ggaggccggc attcttgacc ggcacgactt tggcgatggc cgcgcgcgtt
      601 atgaagccgc gcccgaggcg caccacgacc acatgatcga cgtggagacc ggccgggtga
      661 tcgagttcgt cgatcccgaa ctggaagcgc tccagcgcca gatcgcggag cggctgggct
      721 accggctggt cgaccaccgg atggaactgt tcggcgtgcg catcggccgc gagaaggccg
      781 agggtggctc gggctcctga cccgatgccg gcggaggctt cgctggaccg gccgactccg
      841 gccacacgga tcgggctggc ggcgagcctg cgcatccagg ggcggttgat cctgatcctg
      901 ctgaccgtgc tgcttggcgt accgctgcat tacgtttgcc gcgcgctgac ccgccattcg
      961 ccgatacccc ggctgacgct gcgggcgata gcccggatcg tgggcgcgcg ggtcaggatc
     1021 gtgggcacgc cgctgcggcg cgacgtgttc ctgctggcca accacatttc ctggctggat
     1081 atcccggccc ttgccggggc cagcgggaca gcctttgtcg ccaaggccga agtggcgcag
     1141 gtgccggtgg tcggctggct ggcgcggctc aaccgtaccg tcttcgtcca gcgcgaggca
     1201 cggctcggcg tagcccacca gatcaatgcc ctgcgcgagg ccatggcgga gaatcatgcc
     1261 ctcaccatct tccccgaggg gacgacgacc gacgggcaat cgctgctgcc gttcaagacc
     1321 gccatgctca aggtgctgga gccgccgccg cccggggtca tggttcagcc ggtgctgctg
     1381 gactatggcc ggcttgcccc gttcatctgc tggatcggcg acgagccggg actggccaat
     1441 gtgatcaagg tgctggcccg gccgggctcg tttgcgctga cgatacgctt cctcgagccg
     1501 ttttcacccg agggccatgc cgggcgcaag gccattgcgg cggaggcacg ggcgcggatc
     1561 gaggcggcgc tggtcgaggc cttgggcaag ccgctgcgaa cctacggcgt gaccgtggat
     1621 gcggtgcgct acgcgccgcc gcccgcgccg gcagtggagg cccacgaccg ggccgagggc
     1681 gaggactgaa cgggactcga acccctgccc aaaatcgcct atgggccgcg cccatggcca
     1741 atccctcacc cgccccaaag acctaccgcg tcaagagctt cggctgccag atgaacgtct
     1801 atgacggcga gcgcatggcc gagttgctcg gcagccaggg cattgcgcct gctcccgagg
     1861 gtgaggaggc ggacctggtg gtgctcaaca cctgccacat ccgtgagaag gccgccgaga
     1921 aggtctattc cgacattggc cggatcgtga aggactgggg ccgcgacgat ggctccagcc
     1981 ccctgatcgc ggtggccggc tgcgtcgccc aggccgaggg cgaggagatc atggcccgcg
     2041 cgccggcggt aaagatggtg gtgggcccgc aggcctatca ccg
//
LOCUS       DATNBV010000116         2106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3260558,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000116 DATNBV010000000
VERSION     DATNBV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3260558"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VN627_02820"
     CDS             complement(<1..377)
                     /locus_tag="VN627_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HXD02219.1"
                     /translation="MFKPGQPPRQIECDAALQWLATPAQPNPSEFVWLHLSLSNAAAE
                     RWMRQSLQLADAFYEALHEGVGSTRLEVEGDALVAVMHDVLFNLSFDAANISTVSLCM
                     DPRLVVTARLRPLRSVDRLRASV"
     gene            complement(457..1509)
                     /gene="pstS"
                     /locus_tag="VN627_02825"
     CDS             complement(457..1509)
                     /gene="pstS"
                     /locus_tag="VN627_02825"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008171.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HXD02220.1"
                     /translation="MTLSFIPKVAVILAAAAYGLAASAGEQIRGAGATFPAPVYAAWA
                     KAYQQSNDVQISYEAVGSGGGIERIRQQQVDFGASDAPLTAEELAKAQLIQFPVVIGG
                     VVPVINITGIKPGQLKLSGALLADIYLGRIRKWNDAAIAAINPALALPNTNITVVYRS
                     DPSGSSLLWTDYLSRSSSAWRSGVGASLAPKWPTGVGGTGNEGVASYVQRTRFALGYV
                     EYIYGRRHHLSDVALSNHSGQFVQAGRDAFRAAAETADWNAAAGFQQLPTDRPGAASW
                     PVTGASFILVGKNPSDGRNTTQVLRFFDWALHQGEPIARDLDYVPVPLAMVEQLPALW
                     GTLRDGAGKRLWPQDR"
     gene            1688..>2106
                     /locus_tag="VN627_02830"
     CDS             1688..>2106
                     /locus_tag="VN627_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02221.1"
                     /translation="MLSEDTGTQGRRNAELELGYAWSHQDGTAIFLFQPQLSYGIAST
                     LDLIIQPSWTIVDRPGTGREKGLGDTNLDFKWRFYGAAPWSLGVRAGLGIPTAQEDLG
                     LPGGRVSPHALLVVTGDFTPITFDANLGYTHVPGDLGA"
BASE COUNT          412 a          672 c          643 g          379 t
ORIGIN      
        1 ttgaccgagg cgcgcagccg gtcgaccgaa cgcagcggcc gcagccgcgc ggtgaccacg
       61 aggcgcgggt ccatacacaa cgacacagtc gagatgtttg ccgcatcgaa gctgaggttg
      121 aacaagacgt catgcatcac agcaacgagc gcatcgccct ccacttccag gcgcgtagag
      181 ccgactcctt cgtgcagagc ctcgtaaaag gcatcggcca actgaaggga ctggcgcatc
      241 cagcgctcgg cggccgcatt ggacagcgac aagtgcagcc agacgaactc ggaaggattg
      301 ggctgggccg gcgttgcaag ccattgcaga gccgcgtcac actcgatctg gcggggcggt
      361 tgcccgggct tgaacaaata cccccagacg agcccggttt tgtcggaccc ataggcaagc
      421 tcggccggat ttacatgtag catagttgat tcgagctcaa cgatcctgcg gccataaacg
      481 ctttcccgcg ccgtcgcgca aggtgcccca caaagcgggc agctgctcca ccatggcgag
      541 tggaactgga acatagtcca gatcacgcgc gatgggctct ccctgatgta gggcccaatc
      601 gaaaaaccgt aacacctgcg tggtgttgcg gccatcgctg ggattcttgc cgaccaggat
      661 gaagctcgcg cccgtcaccg gccagctcgc ggctccagga cggtcggtcg gcaactgctg
      721 gaaaccggcc gcggcgttcc aatcagcggt ttcggcagcg gcacggaacg catcgcggcc
      781 cgcctgcacg aactggccac tgtggttgct gagtgcaacg tcgctaagat gatgtctacg
      841 cccatagatg tactcgacgt accccagcgc gaaccgggtc cgctgtacat aggaggccac
      901 gccctcattg cccgttccgc caacgcccgt tggccatttc ggggccagtg atgcacccac
      961 gccagatcgc cacgcactgc tcgatcgcga caggtagtca gtccacagca gagagctgcc
     1021 cgaggggtcg cttcgataca ccacggtgat gttcgtgttt ggcagcgcga gcgctggatt
     1081 gatggcggcg atggcggcgt cattccactt cctgatccgc ccgaggtaga tgtccgccaa
     1141 cagggcaccg ctcaacttca actggccggg cttgatgccg gtgatattga tgaccggcac
     1201 gacgccgccg atgacgacgg gaaattggat caactgcgcc tttgcgagct cttcggcggt
     1261 cagtggcgca tcagaggcgc cgaaatccac ctgctgctgg cgtatgcgct cgattccccc
     1321 accggagccg acggcttcat aagaaatctg tacgtcgttg gattgctggt aggccttcgc
     1381 ccatgcggca tacacgggcg ccggaaatgt cgcccccgcg ccccggatct gctcgccggc
     1441 cgaggctgcc agcccatatg cagcggcagc cagtatcacc gccacctttg gaatgaagct
     1501 caatgtcatg aaggacgatg atgcctgaga gtgcaacaag agcgcgctat gtcatctgat
     1561 cgcattattc gcctgctacg ttggagaatt atttcgagcc agcttggtgc acaaaactcg
     1621 tttgtggggc gcaggagcgt tcggcgcatt ggccctggca acatcggccg cgtacgccgg
     1681 ccaccccatg ctcagcgagg acaccggtac ccagggacgc cggaacgcgg aactcgagct
     1741 gggttatgcc tggagccacc aggatggcac cgccattttc ctgttccagc cgcaactgtc
     1801 ctacggcatc gcctcgaccc tggacctgat catccagcca tcatggacga tcgtcgatcg
     1861 acccggcacc gggcgcgaga agggattggg cgacacaaac ctcgatttca agtggcgttt
     1921 ctatggcgcc gcgccatggt cgctgggtgt gcgggccggc ctcgggatac ccaccgcaca
     1981 ggaggatctc ggcctgcccg gcggcagggt ttccccacat gctctcctgg tcgtgacggg
     2041 cgacttcact cctattacgt tcgacgccaa cctggggtac acacacgttc cgggtgactt
     2101 gggtgc
//
LOCUS       DATNBV010000117         3522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1344701,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000117 DATNBV010000000
VERSION     DATNBV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344701"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VN627_02835"
     CDS             complement(<1..825)
                     /locus_tag="VN627_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02222.1"
                     /translation="MKLLLIVLFLISTASRAEITRIEITSETPFAGGKSFGAVGPYVK
                     VTGRFHGELDPSNPNNKGIADIRSAPRNARGRIEYSADFEVLRPADPAKGNGTLFYDV
                     NNRGNKRIIHLLNDTPASNALDTPETAGDGFLMRHGFSIAWSGWIPAGRSFPAAPNLL
                     RLEVPNAEGIEQPVWDEFLFNDSRQREGKLSFTPASLDKTKAVLTVRDRNDDTPSTIQ
                     RGAWEYVVEGGTAEAIRLLPAGTPFRAGALYQFSYRAKNPPISGIGYAATRDWVAFL"
     gene            complement(844..2127)
                     /locus_tag="VN627_02840"
     CDS             complement(844..2127)
                     /locus_tag="VN627_02840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXD02223.1"
                     /translation="MVKISPALRPVLPPAAVLLAAALALWIGPSLPPSLSGLKEAGAY
                     FVLLAAAGMSLWFNRGRAFVATVSLLLAYTGYRFALDLGADSFAVRAVYTAAVVLVPL
                     NVLVALLLPERGVSYHGDHRWLFIVAGELLLVAWIASSGRNELSGAAWKSMLDHWLLR
                     SPPPPLLGRLLFGVAIAAAAWGAWAPHYSKKGGPVSPLAAGIAGALVAFFVACEWARS
                     EVVFGVFTAAAGAILVVAMLQESHRLAFNDELTGLPGRRALQEAMAGLGPRYVLAMGD
                     VDHFKSFNDTHGHDIGDQVLKLVAARLAEVEGGGRAFRYGGEEFTVLFPDLTLAEAMP
                     HLEAVRISIEGYQMAVRGEDRPKKSEEGEKRRTPGDDDSVPPDKLLSVTISIGGAEPA
                     EHSESGTGMTPAQVLKAADEALYRAKRGGRNRVSR"
     gene            complement(2129..2434)
                     /locus_tag="VN627_02845"
     CDS             complement(2129..2434)
                     /locus_tag="VN627_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HXD02224.1"
                     /translation="MREFARDSRKIVQRISFARLEKDLMRRLALERSLELLGEAARRV
                     SEAAKGEHPGIDWRALIGQRNVLAHDYGEIDHRLLYDNARRKIPLLLAELDRILGEP"
     gene            complement(2463..3095)
                     /locus_tag="VN627_02850"
     CDS             complement(2463..3095)
                     /locus_tag="VN627_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXD02225.1"
                     /translation="MAAAPPGLVNLLFGDYRHRVLSLLFPRPGDRFHVRMISRLTGVP
                     AGSLHRELRQLADAGLLVAERSGNQVLYSANEASPVFHELSSMLDKTAGTPPTLQDHA
                     AEYTIEPTGRLPEIDARALAAICRKYGVKKMSLFGSAARNELRPDSDVDLLVEFKATS
                     KTSTFDLVHMQDELSPLFANRTIQIAGPGILKNPYRRETILPDLKTLYAA"
     gene            complement(3156..>3522)
                     /locus_tag="VN627_02855"
     CDS             complement(3156..>3522)
                     /locus_tag="VN627_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02226.1"
                     /translation="KQGKDFMSVPEGAKVLPPVLVKGGKLVAALSSDARLLVFPIDEL
                     PTRPNGGVGVQIIGLPDKVTLAAVATTDGKSLVVSGIKRKNRAEETLDAKMLKEFEGK
                     RAQRGRVCDVGFRPDRLGS"
BASE COUNT          567 a         1206 c         1171 g          578 t
ORIGIN      
        1 caggaaggcg acccagtcgc gcgtggcggc gtagccgatg cccgagatgg gcgggttctt
       61 cgcgcggtag gagaactggt acagcgcgcc cgcacggaac ggcgtgccgg ccggcagcag
      121 gcggatggct tccgcagtgc cgccctcgac cacgtactcc caggccccgc gctggatggt
      181 gctgggcgtg tcgtcgttgc ggtcgcgcac cgtgagcacg gccttggtct tgtccaggct
      241 ggccggcgtg aaggacagct tgccctcgcg ctggcgcgag tcgttgaaga ggaactcgtc
      301 ccataccggc tgctcgatgc cctcggcgtt cggcacttcc agccgcagca ggttcggcgc
      361 ggccgggaag ctgcgcccgg ccgggatcca gccgctccag gcgatggaga agccgtggcg
      421 catcaggaag ccgtcgccgg cggtttcggg cgtgtcgagc gcattgctgg ccggggtgtc
      481 gttcagcaga tggatgatcc gcttgttgcc gcggttgttg acgtcgtaga acagcgtgcc
      541 gttgcccttg gccgggtcgg cgggccgcag cacttcgaag tcggcggagt actcgatgcg
      601 cccgcgcgcg ttgcgcggcg cgctcctgat gtcggcgatg cccttgttgt tcgggttgct
      661 gggatcgagc tcgccgtgga aacgccctgt caccttgacg taggggccga ccgcgccgaa
      721 ggacttgccg ccggcgaagg gcgtctccga tgtgatttcg atgcgggtga tttcagcccg
      781 ggatgcagtt gaaatcagga acagaacgat caaaagcagt ttcatgccga ccccctgccc
      841 tgattatctg ctcacgcggt tcctcccgcc acgcttggcg cgatacagcg cctcgtcggc
      901 cgccttcagc acctgggccg gggtcatccc cgtgccggac tcgctatgct cggcgggctc
      961 ggcgccgccg atggaaatgg tcaccgacag caacttgtca ggcgggaccg agtcgtcgtc
     1021 gcctggggtt cgccgtttct cgccctcttc cgacttcttc ggccggtctt cgccgcgcac
     1081 cgccatctgg taaccctcga tcgagatgcg taccgcctcc aggtgcggca tcgcctcggc
     1141 cagcgtcagg tccgggaaca gcacggtgaa ttcctcgccg ccgtagcgga aggcgcgccc
     1201 gccgccctcg acctccgcca ggcgcgctgc gaccagcttg agcacctggt cgccgatgtc
     1261 gtggccgtgc gtgtcgttga aggacttgaa gtggtccacg tcgcccatgg cgagtacgta
     1321 gcgcggcccg aggcccgcca tcgcctcctg aagcgcgcgc cggcccggca ggccggtgag
     1381 ttcgtcgttg aaggcgagcc ggtgcgactc ctgcagcatg gccaccacca ggatcgcccc
     1441 cgcagccgcg gtgaacaccc cgaacaccac ctcgctgcgc gcccattcgc aggcgacgaa
     1501 gaacgccacc agcgcccccg cgatgcccgc cgccagcggc gacaccgggc cgcccttctt
     1561 cgagtagtgc ggcgcccagg cgccccaggc ggcggcggca atcgccacgc cgaacagcag
     1621 ccggccgagc agcggcggcg gcggcgagcg cagcagccag tgatcgagca tcgacttcca
     1681 cgcggcgccc gaaagctcgt tgcgccccga ggaggcgatc caggccacca gcagcaactc
     1741 gccggcgacg atgaacagcc agcggtggtc gccgtggtac gacaccccgc gctcgggcag
     1801 cagcagcgcc accagcacgt ttagcggcac cagcaccacc gccgccgtgt acaccgcccg
     1861 cacggcgaag ctgtccgctc ccaggtcgag cgcgaagcgg taccccgtgt aggccagcag
     1921 cagcgacacc gtggcgacga acgcccgccc gcggttgaac cacaggctca tgcccgctgc
     1981 ggccagcaac acgaagtacg cccccgcctc cttcagcccc gacagcgaag gcggcagcga
     2041 aggcccgatc cagagcgcca gcgcggcggc cagcaggacg gcggccggcg gcaggacagg
     2101 gcggagggct ggcgaaatct tgaccatgtc agggctcgcc cagaatgcgg tcgagctcgg
     2161 ccagcagcaa aggaattttt cgccttgcat tgtcataaag caggcgatgg tcgatttcgc
     2221 cgtagtcgtg cgccagcacg tttcgctggc cgatgagcgc ccgccagtcg atgcccggat
     2281 gctctccctt cgcggcttcg ctgacgcgcc gggcggcctc cccaagcaac tcgagcgagc
     2341 gctccagcgc gagcctgcgc atcaagtcct tttccaggcg ggcaaacgaa atcctctgga
     2401 cgatcttgcg ggaatcccgc gcaaactcgc gcatgtccca gaggtaggcg cggtcgcgcc
     2461 tttcaggccg catacagcgt cttgaggtcc ggcaggatcg tctcgcgccg gtacgggttc
     2521 ttcagtatcc cggggccggc gatctggatc gtccggttcg cgaacagcgg cgacagttca
     2581 tcctgcatgt gcacgagatc gaacgtcgag gtcttgcttg ttgccttgaa ctcgaccagg
     2641 agatccacgt cgctatcggg acgcaattcg ttgcgcgccg ccgaaccgaa cagcgacatc
     2701 ttcttcacgc cgtatttgcg gcagatcgcg gccagggccc tggcatcgat ttcgggaagc
     2761 cggcccgtgg gctcgatggt gtattccgct gcgtgatcct gcagcgtggg cggcgtcccg
     2821 gcggtcttgt cgagcatgga ggagagttcg tggaacaccg gcgacgcctc gttcgccgag
     2881 tagagaactt ggttgccgct gcgctcggca acgagcaatc cggcgtccgc gagctggcgc
     2941 aattcccggt gcagggaacc ggctggaacc ccggtcaggc gtgaaatcat gcgcacatgg
     3001 aaccggtcgc caggccgggg gaaaagcagc gacagcacgc gatggcggta gtcgccgaac
     3061 aacaggttca ccaagcccgg cggggcagct gccacttcgg attccgttct caccattgct
     3121 acgattgtag catcaatgag aacgaatggc ctgggctagc tccccaagcg atccgggcgg
     3181 aagccgacgt cgcagacccg cccgcgctgg gcccgtttgc cctcgaattc cttgagcatc
     3241 ttggcgtcca gggtctcttc ggcgcggttc tttcgcttga tgccggagac caccaggctc
     3301 ttgccgtcgg tggtcgccac ggcagccagg gtcaccttgt ccggcaggcc gatgatttgc
     3361 acccccaccc cgccgttcgg gcgggtcggc agttcgtcga tcgggaacac cagcagccgg
     3421 gcgtccgagg aaagcgccgc aaccagcttg ccgcctttga ccagcacagg cggcaacact
     3481 tttgcccctt ccgggacgct catgaagtct ttgccctgct tg
//
LOCUS       DATNBV010000118         3376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4221230,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000118 DATNBV010000000
VERSION     DATNBV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4221230"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VN627_02860"
     CDS             <1..748
                     /locus_tag="VN627_02860"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD02227.1"
                     /translation="LPGGSSLLAEDADVREAYPVLDTTAAGYATGYGMSKWAGEVLLR
                     NAHEAFGLPVSIFRCGMILAHSRYAGQLNLPDMFTRLLLSISLTGLAPATFYREGVSS
                     PHYDGLPVDFMAGAINELGLHARSGIQTFHVVNPHDDGVSMDTFVDWIAATGRQISRI
                     ADYADWLDRFTTALKALPEHQRQRTSLPLLHSIQKQERGHALVPASRFREGIRQRGLG
                     EDGDIPHLSATLIAKYLRDLEVTSMLPESI"
     gene            835..3030
                     /locus_tag="VN627_02865"
     CDS             835..3030
                     /locus_tag="VN627_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HXD02228.1"
                     /translation="MEQRLFVCRSCNASCPIVITLDGDRPIKVEGDHDAPLYRGYTCP
                     KGRAIPEAHGNPNRLLHSLKRLPDGTRVPISSEQLVEEIAARLERIIDAHGPNAVATF
                     LGTPLTAQPTSKITMVSFVRAIGSKMFFSPMTIDQPGLLISQALHGTWQGGRMHPDQW
                     ETLLLIGGNPIVSKQYLPQNPAWQLKELQKRGLRMVVIDPRRTETARRAAVHLQAIPG
                     EDPTVLAGLIHLVFALGGLDEAFLAENAEGVDALRSAVAEFTPDYVAARAGLAVDDLV
                     AAARILVESRGGDTAPGIGSNMATRGNLMSYLTLCLQTLRGFWSRERDPVSRPRVLTP
                     RREWRAQPKAPRPAWFGAPVSKYNYRLTVAGMPAAALPELMLSDGPDRVRALFLHGSA
                     YNCWPDTAKTVRALGELDLLVMHDIEISATSAHADYIIATYDQLETSAVSAANEAVGE
                     LHPGYDWNEPYAYYRKALLKPPAGSDLMESWQIYYRVAQKLGLSLSHVAGPGPDDRIA
                     FDMTTEPDTEYLVELLCHGSAVPLEEVKRHPHGGIFETARDQVKPRDPECTDRLQLGD
                     PTMLAELAELRAEDPLGRRKTDNEFPFLFIGRRIQGATNSAPRPRGVVRTGYNPLWMN
                     PADMVRIGVDEGGEVAIRSRHGEIVGFVEADADMREGVVGMTHGFGPKPGIPYDPRRD
                     GSNVNELISWEDDADPYHGMPRMSALPVSVLARAGRAAATAAGELALPG"
     gene            complement(3051..>3376)
                     /locus_tag="VN627_02870"
     CDS             complement(3051..>3376)
                     /locus_tag="VN627_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02229.1"
                     /translation="RTIGNLPFGKWMREKLPPERVSPVVAFLAHESCPVTGQFISAAG
                     GRVTRIVYASSHGYFNPDLTVEDVRDNWATITGEADDAGGLAGYFELEGQSSEFKEIL
                     KLVGK"
BASE COUNT          576 a         1145 c         1101 g          554 t
ORIGIN      
        1 ccttcccggc gggtcgtcat tgctcgccga ggatgccgat gtgcgggaag cctatccggt
       61 gctcgacacc acggcggcgg gctatgcgac cggttacggg atgagcaagt gggccggcga
      121 agtgctgctg cgcaatgccc acgaagcgtt cggcctgccg gtatcgatct tccgctgcgg
      181 gatgatcctc gcgcacagcc gctatgccgg ccagctcaat ctgcccgaca tgttcacgcg
      241 cctgctgctg agcatttccc tcacgggcct tgccccggcc acgttctacc gcgagggcgt
      301 ctccagcccc cactatgacg gactgccagt ggacttcatg gccggggcaa tcaacgagct
      361 gggcctgcat gcccgatcgg ggatccagac ctttcacgtg gtcaatccgc atgacgacgg
      421 cgtatcgatg gacacgttcg tcgattggat agcggcaacg ggtcggcaga tctcgcgaat
      481 tgcagactat gccgactggc tcgaccggtt cacgaccgcg ctgaaggccc tgcctgagca
      541 ccagcgacag cgcacctccc tgccgctgct ccacagcatc cagaagcagg agcgtggtca
      601 cgcgctggtc ccggccagtc gcttccggga ggggataagg caacgcgggc tcggtgagga
      661 cggcgacatc ccgcacctgt cggcgacctt gattgcgaag tacctgcgcg acctcgaagt
      721 caccagcatg cttccggaga gcatttgaat gagccgcata tagcagcggc tggccaatgg
      781 ccgccgggct gggattgagg acggggaata tcccccgcca ggagtgacac gacgatggaa
      841 cagcgcctct ttgtatgtcg cagttgcaat gccagctgcc cgatcgtgat cacgcttgat
      901 ggagaccggc cgatcaaggt ggagggcgat cacgatgccc cgctttatcg cggctacacc
      961 tgccccaagg gccgcgcgat cccggaggcg cacgggaatc cgaaccgcct gctgcacagc
     1021 ctcaagcgcc tgccggatgg cacccgcgtg ccgatctcca gcgaacagct ggtcgaggag
     1081 atcgccgccc ggctggagcg gatcatcgat gcccatgggc cgaacgccgt cgccacgttc
     1141 ctcgggacgc cgctcaccgc gcagccgacc tccaagatca cgatggtcag tttcgtgagg
     1201 gccatcggct cgaagatgtt cttttcgccc atgacgatcg atcagccggg tttgctgatc
     1261 tcgcaggcgc tgcatggcac ctggcagggt ggccgcatgc atcccgatca gtgggaaacc
     1321 ttgctcctga tcggcggaaa cccgatcgtg tcgaagcagt acctgccgca aaatccggca
     1381 tggcagttga aggaactgca aaagcgcggc ctgcgcatgg tggtcatcga tccgcggcgc
     1441 accgagacgg cgcggcgggc ggcggtccac cttcaggcga tcccgggcga ggacccgaca
     1501 gtccttgctg gcctcatcca tctggtcttc gcactgggcg gccttgacga ggcattcctg
     1561 gccgagaacg cggaaggggt cgatgcgctg cgcagcgcgg tcgccgaatt cacgccggac
     1621 tacgttgcgg cgcgggccgg cctcgccgtg gacgatctgg tcgcggccgc gcgcatcctg
     1681 gtcgaatcgc gcggcggcga taccgccccc ggcatcggct ccaacatggc gacccgcggc
     1741 aacctgatga gctacctgac gctctgcctg cagaccctgc gcggcttctg gtcgcgcgag
     1801 agggacccgg tttcacggcc gcgggtgctc actccgcggc gcgaatggcg tgcccagccc
     1861 aaggcgccgc gccccgcctg gttcggcgcg ccggtctcga agtacaatta ccggctgacc
     1921 gtggccggca tgcccgctgc cgccctgccg gagctgatgc tgagcgatgg cccggaccgg
     1981 gtgcgcgccc tgttcctcca cggcagcgcc tacaattgct ggcccgacac ggccaagacc
     2041 gtgcgggcgc tcggcgagct cgacctgctc gtcatgcacg acatcgaaat ctcggcaacc
     2101 tcggcccatg ccgactacat catcgcgacc tacgaccagt tggagacctc ggccgtttcg
     2161 gcggcgaacg aggcggtggg cgagcttcat cccggctacg actggaacga gccctatgcc
     2221 tactaccgca aggcgctgct gaaaccgccg gccggctcgg acctgatgga aagctggcag
     2281 atctactatc gcgtggccca gaagctcggc ctgtcactgt cgcacgtcgc gggccccggt
     2341 ccggacgacc ggattgcgtt cgacatgacc acggaacccg acacggagta cctggtcgag
     2401 ctgctctgcc acggcagcgc cgtgccgctg gaagaggtca agcggcaccc gcacggcggg
     2461 atcttcgaga cggcgcgcga ccaggtcaaa cctcgcgacc ccgaatgcac cgaccgcctc
     2521 cagcttggcg acccgaccat gctggcggaa ctggcggaac tgcgggccga ggatccgttg
     2581 ggccgccgca agaccgataa cgagttcccg ttcctgttca tcggccgccg catccaggga
     2641 gcgaccaaca gcgccccgcg cccgcggggg gtggtgcgca ccggctacaa tccgctgtgg
     2701 atgaacccgg cggacatggt ccggatcggt gttgatgaag gcggcgaggt ggcgatccgc
     2761 tcgcgccacg gcgaaatcgt gggatttgta gaagccgatg ccgacatgcg cgaaggcgtc
     2821 gtcggcatga ctcacggctt cggccccaag cccggcattc cgtacgatcc gcggcgcgac
     2881 ggctccaacg tcaacgagct gatcagctgg gaagacgatg cggatcccta tcacggcatg
     2941 ccccggatga gcgcgcttcc cgtgtcggtc ttggcgcggg ccgggcgagc cgcggcaacc
     3001 gctgccggcg aactcgccct gccgggctga cgttcgcctc cgaccgggcc ctacttgccg
     3061 accagcttca ggatctcctt gaattccgat gactggcctt ccagctcgaa atacccggcg
     3121 aggccgcccg catcgtcggc ctcaccggtg atcgtcgccc agttgtccct gacgtcctcg
     3181 accgtgaggt cgggattgaa atagccgtgc gacgaggcgt agacgatccg tgtgacgcgt
     3241 cctcccgcag cggatatgaa ctgcccggtg acggggcagc tctcatgcgc gaggaatgcg
     3301 acgactggcg acacccgctc gggcgggagt ttctcccgca tccattttcc gaacggcagg
     3361 ttgccgatcg tgcggt
//
LOCUS       DATNBV010000119         5005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_715039,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000119 DATNBV010000000
VERSION     DATNBV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715039"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VN627_02875"
     CDS             complement(<1..405)
                     /locus_tag="VN627_02875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXD02230.1"
                     /translation="MADLVLRSPTMADVPALSLLGVAAFVHKFGDLYRPEDLLPFLAE
                     THSEAALAMAIADPLTSYQVAERGGLLLGWAKLGLTCSFPDHARGARAMELKQLYTAP
                     NAQGAGIGAALMDWAMAEFAALGADEVQLSVWR"
     gene            complement(398..1282)
                     /locus_tag="VN627_02880"
     CDS             complement(398..1282)
                     /locus_tag="VN627_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXD02231.1"
                     /translation="MITPEGVPLEFVLAPRGSRAGALFIDFVLIGLVMVVTSAALLYL
                     GIGVFGLAGIEGRNDAVGRALQFLAVIWIMAMFLLRNAWFLFFELGPRGATPGKRSQR
                     IRIAARDGGRLTAEMVIARNLLRDVELFLPASFLIAAKNGGGDLGPAGLAAAGWFLIF
                     ALFPFFNRDALRAGDLIAGTWVVEAPRMRLAPALSRAVAGPSAGYRFGEAELSVYGEY
                     ELQVLERVLREDRPESLAAVYQRICTKIGWDAGTADERAFLEAYYTQLRARLESGMRL
                     GRRKADKFAGTGSGPGRG"
     gene            complement(1383..2465)
                     /locus_tag="VN627_02885"
     CDS             complement(1383..2465)
                     /locus_tag="VN627_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HXD02232.1"
                     /translation="MALFETLRAVRLPGLFGRAPDAAHDDAIAAAALRSDRFREAREH
                     EWQRLERIVGRLEAGSLRSLSDDDLLALPVLYRTAASSLSVARETSLDAATLAYLEAL
                     VQRAWFQVYGPRQGLGTWLARFLGGGWSAAVRGLGLDLAIAFAVMVAGTIVGWLLVAS
                     DPSWYGALFPNEGMDPRVPGATREALHATLFGQSGESGLGMFAAYLFSHNAQIAILAF
                     ALGFAFGVPSLLLLIQNTATLGAMIWLFHGQGLTIEVIGWLCVHGTTELFAILLAGAA
                     GLHIGRAIAFPGERSVLDSVAAAGRRAALVMLGVVLMLMVAGMLEGFARQLIDDTTAR
                     LTLGWSVLALWLAYFFAFCRTPGRLS"
     gene            complement(2465..3802)
                     /locus_tag="VN627_02890"
     CDS             complement(2465..3802)
                     /locus_tag="VN627_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXD02233.1"
                     /translation="MNLTFAPRLAIVPTQRAAVLIGLAAPLALVIAAAAPGAWLVAPA
                     LGLALLLLVLADALLAGRLADLRLVLPADGEVGEPMPLTVLADLPEGRISSAQAALEC
                     DPRLAPGGRLVLPLTPADGAWQGHASLVPSRRGPAPVSRLWLRWTGPLGLGGRQHSRP
                     LDRTLAVWPSLAAARSPALQLFLRDSEHGMIARRIRGEGSQFEALAEYQPGMDRRRID
                     WKTSARHTHLYAREYEAERNNPIVFAFDCGRAMCEPVAGLPRIDRAISAALAAAWVAL
                     KGGDRVALYGFAARPLAATPFVTGTPGFARLQRAAATLDYRAEEPNFTLALATLAERL
                     QRRSLVVVFSEFTDPTSAELLLESVGRLTRRHVVLFVTLADEELEAVAGEAPEDMARL
                     AMAVGAEALLRTRGLVLARLRQMGVEVIEAPHDRLGTRLIDAYLALRRKGAVG"
     gene            complement(3819..4790)
                     /locus_tag="VN627_02895"
     CDS             complement(3819..4790)
                     /locus_tag="VN627_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXD02234.1"
                     /translation="MSESALEVADVARLADAIRGEIARAIVGLDHTVDHLLVALLAGG
                     HVLLEGPPGTAKTFLAQCFSTAQGRDFGRIQFTPDLMPADILGSNLFNFQTSQFTLTR
                     GPVFCELLLADEINRTPPKTQAALLEAMQEGRVTLDGETHVLPERFMVVATQNPIESQ
                     GVYPLPEAQLDRFLFKLLIPYPSLAEETKIVARFGEGSGPPRAADLGVSAVADRATLR
                     AAAAAVRSITLADSVTDYVVRLVRATRESADIATGASPRAAVLLAGAARARAALAGRD
                     YVLPDDVKALATAVLRHRLLLSPAAEIEGKEIEAIVEDLVQQTEAPR"
     gene            complement(4787..>5005)
                     /locus_tag="VN627_02900"
     CDS             complement(4787..>5005)
                     /locus_tag="VN627_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HXD02235.1"
                     /translation="ALVRERLVRALALPHRADPAAHEAAIDRALAMRAPGSPAFSTAA
                     AALRTARRPAELLRAAQDLHAIERMLER"
BASE COUNT          820 a         1584 c         1981 g          620 t
ORIGIN      
        1 ccgccagacg gagagctgga cctcgtcggc gccgagcgcg gcgaactcgg ccatggccca
       61 gtccatcagc gcggcgccga tcccggcccc ttgcgcattg ggcgcggtgt agagctgctt
      121 cagctccatg gcccgcgcgc cgcgggcatg atccgggaaa gagcaggtga ggccgagctt
      181 ggcccagccg agcagcaggc ccccgcgctc ggcaacctga tagctggtca gcgggtcggc
      241 gatggccatg gcgagggcgg cttcggagtg ggtttcggca aggaacggca gcaggtcttc
      301 cgggcggtac agatcgccga acttgtgcac gaaggcggcg acgccgagca gcgagagggc
      361 ggggacatct gccatggtgg ggctgcgcag gacgaggtca gccacggccg ggtccgctgc
      421 cggtgccggc gaacttgtcc gcctttcgcc ggccaaggcg catgccggat tccagccggg
      481 cgcgcagctg ggtgtaatag gcctcgagga aggcgcgctc gtcggcggtg ccggcatccc
      541 agccgatctt ggtgcagatc cgctggtaga ccgcggcgag gctttccgga cgatcctcgc
      601 gcaggacccg ttcgaggacc tggagctcgt attcgccata gaccgacagc tcggcctcgc
      661 cgaagcggta gcctgccgat ggcccggcga cggcgcgcga gagggcaggg gcaaggcgca
      721 tgcggggggc ttcgaccacc caggttccgg cgatgagatc gcccgcgcgc agggcatcgc
      781 ggttgaagaa tggaaagagg gcgaagatca ggaaccagcc cgccgcggcc agcccggcgg
      841 ggccgagatc gcctccgcca ttctttgcgg cgatcagaaa gctggctggc aggaacagtt
      901 cgacgtcgcg caggagattg cgggcgatca ccatttcggc ggtaagccgg ccgccatcgc
      961 gcgcggcaat gcggatgcgc tgcgagcgtt tgcccggcgt ggccccgcgc gggcccagtt
     1021 cgaagaacag gaaccaggcg ttgcgcagca ggaacatggc catgatccag atcacggcaa
     1081 ggaactgcag ggcgcggcca acggcatcgt tgcggccctc gatcccggcc aggccgaaga
     1141 ccccgatccc gagatagagc agggccgcgc tggtcacgac catgacgagg ccgatcagca
     1201 cgaaatcgat gaacagcgcc ccggcgcgcg agccgcgtgg agcaaggacg aattcgaggg
     1261 gcacgccttc gggggtgatc accgtgcggc ggcggcgagc gagatcggcg cgggcaaggt
     1321 ctgccccgtc ggcgcgccgg cgctcgcggc gaaggaaggt gggactcttc acggcagccg
     1381 gctcatgaca ggcgccccgg ggtgcggcag aacgcgaaaa agtaggcgag ccacagggca
     1441 aggaccgacc agccgagggt caggcgggcg gtggtatcgt cgatcagctg ccgcgcgaag
     1501 ccttccagca tcccggcaac catcagcatc aggacgacgc cgagcatgac cagcgccgcg
     1561 cgccgcccgg ctgcggccac ggaatcgagc acggagcgtt ctcccgggaa ggcaatagcc
     1621 cggccgatgt ggaggcccgc cgcgccggcg agcaggatgg cgaagagctc ggtcgtgcca
     1681 tggacgcaga gccagccgat cacctcgatg gtcagcccct gcccgtggaa cagccagatc
     1741 atggcgccaa gcgtggccgt gttctggatc agcaggagga gcgagggcac cccgaaagcg
     1801 aagccgaggg caaaggcgag gatcgcgatc tgggcattgt ggctgaacag gtaggccgcg
     1861 aacatgccga ggccggattc gcccgactgg ccgaacaggg tggcatgaag cgcctcgcgg
     1921 gtggcgccgg ggacgcgcgg atccatgcct tcattgggaa acagcgcgcc gtaccacgag
     1981 ggatcggagg cgacgagcag ccagccgacg atcgtgccgg cgaccatcac cgcgaaggcg
     2041 atcgcaaggt ccagccccaa gccgcgcacg gcggccgacc agccaccgcc gaggaaccgc
     2101 gccagccagg tgccgaggcc ctgccgcggg ccgtagacct ggaaccaggc gcgctgcacg
     2161 agggcttcga gataggccag cgttgccgca tcgagcgagg tttcgcgggc gaccgagagg
     2221 ctggaggcgg cggtgcggta gagcacggga agggcgagga ggtcgtcgtc cgacaggctg
     2281 cgcagggagc ccgcttcgag ccggccgacg atccgctcca gccgctgcca ttcgtgctcc
     2341 cgggcctcgc ggaagcggtc ggagcgaagg gcggcggcgg cgatcgcatc gtcgtgcgcg
     2401 gcgtcggggg cgcggccgaa caggccgggc agacgaaccg cgcgcagggt ttcgaacagg
     2461 gccatcagcc gacggctccc ttgcggcgca gggccagata ggcatcgatc aggcgggtgc
     2521 cgagccgatc gtgcggggcc tcgatcacct cgacgcccat ttgcctgagg cgggccagga
     2581 caagaccgcg cgtgcgcagc aaggcctcgg cgccgaccgc catggcgagg cgggccatgt
     2641 cttccggtgc ctcgccggcg accgcttcga gttcctcgtc ggcgagggtg acgaacagga
     2701 cgacgtgacg cctggtcagc cggcccacgc tttcgaggag cagttcggcg ctggtcggat
     2761 cggtgaattc ggagaatacc acgaccagcg agcggcgctg gagccgttcg gccagggtcg
     2821 cgagggcgag ggtgaaatta ggctcctcgg cccggtagtc gagcgtggcg gcggcgcgct
     2881 ggaggcgggc aaagcccggg gtgccggtga caaagggcgt cgcggcgagc ggccttgcgg
     2941 caaagccgta gagggcgacc cggtcacccc ccttgagcgc gacccaggcg gcggcgaggg
     3001 cggcggaaat ggcccgatcg atccggggga gcccggcgac cggttcgcac atggcgcggc
     3061 cgcagtcgaa ggcgaagacg atggggttgt tgcgctccgc ctcgtattcg cgggcgtaga
     3121 ggtgggtgtg gcgggccgag gttttccagt cgatccggcg acgatccatc cccggctggt
     3181 attcggcgag cgcctcgaac tggctgcctt cgccccggat acgacgggcg atcatgccgt
     3241 gttcgctatc gcgcaggaac agctggagcg cgggcgagcg ggcggcggcg aggctgggcc
     3301 atacggcgag ggtgcggtca agcgggcggc tgtgctggcg cccgccgagg ccgagcgggc
     3361 cggtccaccg cagccacagt cgcgagacgg gcgctgggcc gcggcggctg ggcacgagcg
     3421 aggcatggcc ctgccaggcg ccgtccgctg gggtgagggg gaggacaaga cgaccgccgg
     3481 gggcgaggcg ggggtcgcat tcgagcgcgg cttgggccga gctgatgcgg ccctccggca
     3541 agtcggccag cacggtgagc ggcatgggct cacccacttc gccgtcggcg ggaaggacca
     3601 gcctgaggtc cgcgagacgc ccggcgagga gggcatcggc gagaaccagc agcaggaggg
     3661 caaggcccag cgccggggcg accagccacg cgcccggagc ggcggcggcg atcaccagcg
     3721 caagcggcgc ggcgaggccg atcagcacgg cggcgcgctg ggttggcacg atcgccaggc
     3781 ggggggcgaa ggtgaggttc atggggcggg ggctggcttc accgcggcgc ttccgtttgc
     3841 tggacgagat cctcgacgat ggcttcgatt tccttgccct cgatctcggc ggcggggctg
     3901 agcaggagac ggtggcgcag gacggcggtg gcgagcgcct tgacgtcgtc gggcagcaca
     3961 tagtcgcgtc cagccagcgc cgcgcgggcg cgggcggcac cggcgaggag cacggcggcg
     4021 cgggggctgg cgcccgtggc gatatcggcg ctctcgcggg tcgcgcgcac gaggcgcacg
     4081 acatagtcgg tcacgctgtc ggccagcgta atcgaccgca cggcggcggc agcggcgcgc
     4141 agggtggccc ggtcggccac ggcgcttacc ccgaggtcgg cggcgcgcgg cggcccggag
     4201 ccttcgccga agcgagcgac gatcttcgtc tcctcggcga ggctgggata ggggatcagc
     4261 agcttgaaca ggaagcggtc aagctgggcc tcgggcaggg gatagacgcc ctggctttcg
     4321 atcgggttct gcgtggcgac gaccatgaac cgctcgggca ggacatgggt ttcgccgtcg
     4381 agggtgacgc ggccttcctg catggcttcg agcagggcgg cctgggtctt gggcggggtg
     4441 cggttgatct cgtcggccag cagcagctcg cagaacaccg gaccccgggt gagggtgaac
     4501 tggctggtct ggaagttgaa caggttggag ccgaggatat cggcgggcat cagatcaggc
     4561 gtgaactgga tgcggccgaa atcacggccc tgagcggtgg aaaagcactg ggcgaggaag
     4621 gtcttggccg tgcccggggg gccttcgagg aggacatggc ccccggcgag cagggcgacc
     4681 agcaggtggt cgaccgtgtg atcgaggccg acgatcgccc tggcgatctc gccccggatc
     4741 gcatcggcga ggcgggccac atcagccacc tccagcgcgg attcgctcat cgttccaaca
     4801 tcctttcgat agcgtggagg tcctgcgcgg cgcgcaggag ctctgccggg cggcgcgcgg
     4861 tgcgcagggc cgcggcggcg gtggagaagg cgggcgagcc gggcgcgcgc atggccagcg
     4921 cccggtcgat cgcggcctcg tgggcggcgg gatcggcgcg gtggggcagg gcgagggcgc
     4981 ggaccagccg ctcgcgcacc agcgc
//
LOCUS       DATNBV010000120         3909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_715427,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000120 DATNBV010000000
VERSION     DATNBV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715427"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VN627_02905"
     CDS             complement(<1..326)
                     /locus_tag="VN627_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXD02236.1"
                     /translation="MDGLMSFTLPLSRDVFFIGGEWVAPSSPEMISIISPRTEEVVGT
                     VPAGQRGDIDRAVAAARKAFDHGPWPRMSVEERGAIMRKVAAYMRERGPEVAKLVSTE
                     MGGPMS"
     gene            complement(390..515)
                     /locus_tag="VN627_02910"
     CDS             complement(390..515)
                     /locus_tag="VN627_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02237.1"
                     /translation="MTEEWLARIPDFALDPDRPPALGSGIVSTMNALPLVRVAGP"
     gene            complement(512..853)
                     /locus_tag="VN627_02915"
     CDS             complement(512..853)
                     /locus_tag="VN627_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02238.1"
                     /translation="MSFVFLYLARHPEPRATLRARPDLIVNAADELIRRHGLVASTGV
                     LTRDVEMHGAAMKADEHIILPNSFAGLDTTLFPDPWTVDIERKGWPARGLPQWAPPLL
                     ASASRGWKSGS"
     gene            complement(937..1083)
                     /locus_tag="VN627_02920"
     CDS             complement(937..1083)
                     /locus_tag="VN627_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02239.1"
                     /translation="MASNLNVLGDADSAPIVDFDMYRPPALQQGLQEAWQTLADALDR
                     PVPR"
     gene            complement(1186..2673)
                     /locus_tag="VN627_02925"
     CDS             complement(1186..2673)
                     /locus_tag="VN627_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXD02240.1"
                     /translation="MATLLERSEAPAFLQGTKLLLIDGKHIAAQSGKTFEVVNPATEE
                     VIATVAEGDAADIALAVSAARKSFESGVWRSLPASERARIMLRFADLVEENGDELSLL
                     ETMNNGMPRAFARNSVHGGAKWLRWFAGSVTRIYGQTASSAVSAPGEFHVYTRKEPIG
                     VVGLITPWNGPIGTFFIKVAPALASGCSIVQKPSEITPLTALRLGELALEAGVPAGVL
                     NVVPGFGAGAGAAMANHPGINKISFTGSTAVGKELVRASAGNLKRVTLELGGKSPCVV
                     FDDADMDIAIPGAAMAIAANTGQVCFAGSRLFVQRKSFDKVVAGIGRVLQSLKVGNGL
                     EEDTVLGPVVSAKQRDRVTGYIQTGLSEGAEIVSGGQPIEGKGFFVSPTVFANVHANM
                     KIVQEEIFGPVLVATPFDDLEEVPALANATNYGLGGGIYTTNINTAHRLAARIETGNV
                     WINCYSMLDAAMPFGGYKESGWGRELSAEGLDAYLETKSVWVKLS"
     gene            complement(2837..>3909)
                     /locus_tag="VN627_02930"
     CDS             complement(2837..>3909)
                     /locus_tag="VN627_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HXD02241.1"
                     /translation="GFPKGVVHSQHNMKVTATISVEHSGDGVPETVRGTALPLTILNV
                     MILGPLAALSTGAVHVNLDRIDAQGVAEWIGQEKIANISLVPTLVQDLLTRPDIDQEQ
                     LRSLRWLVVGGSTVPQSLPQLYKERFGRRMTVGYGLTEGPNGVAKTHENSPEETGVIG
                     RPLPHLHLAILDKEGREAPRGEVGEIAIRATDTGPWAGIYKPTLGYWKRPESTAKLLQ
                     DGWVHTGDVGVQDESGEFHIRDRGSDLILRGGANVYPAEVERVLRLDPQVRDCAVVGK
                     PHARLGQTVAAFIETESGPESERQALIERLQGLCTTNLAAYKAPVDWIVLPAMPRNAM
                     GKIVKAQLVQQLAGSVEEPQTA"
BASE COUNT          630 a         1298 c         1275 g          706 t
ORIGIN      
        1 atgctcatag ggccgcccat ctcggttgag accagtttgg cgacctccgg cccgcgctcg
       61 cgcatatagg ccgcgacctt gcgcatgatt gcgccgcgct cctccaccga catgcgcggc
      121 cagggaccat ggtcgaacgc cttgcgcgca gcggcgacgg cacgatctat gtcgccgcgc
      181 tggccggcgg gcacggtgcc gaccacttct tccgtccggg gcgagatgat gctgatcatc
      241 tcgggcgagg agggggcgac ccattccccg ccaatgaaaa agacatcacg cgaaagcgga
      301 agagtaaagc tcatcagtcc atccatttac taccgtattt tttgattctt ttatctctat
      361 ttgacggcat tgcaacggcg ggctggtggc tatggtccgg ccacccggac cagaggcagc
      421 gcattcatcg tgctgacgat gccgctgccc aatgccggcg gccggtcggg atcgagcgcg
      481 aagtcaggga tgcgcgccag ccattcctcg gtcacgagcc ggatttccag ccgcgcgagg
      541 ctgacgccag cagcggtggg gcccattgcg gaaggccgcg tgccggccag cccttgcgct
      601 cgatgtcaac cgtccagggg tcggggaaga gtgtcgtgtc cagtccggcg aagctgttgg
      661 gcaggatgat gtgctcgtcg gccttcattg ccgcgccgtg catttcgaca tcgcgcgtca
      721 gcaccccagt ggaggcgacc aggccgtggc ggcggatcag ttcgtcggca gcgttgacga
      781 tgaggtcggg ccgcgcccgc agcgtcgcac gcggctccgg gtggcgcgcg agatagagga
      841 agacgaagga catcatcgac gccaccgtgt agagtccacc agacgtgacc gccgccggcc
      901 gctaccgccg ccccggtcgc tatccagtgc ccgccattat cgggggaccg gacgatcgag
      961 ggcatcggcg agcgtctgcc atgcttcctg gaggccctgc tgcagcgccg gcgggcggta
     1021 catgtcgaag tcgacgatcg gggcggaatc ggcatctcct agcacattca agttgctcgc
     1081 catggcatcc tccccgaccc agatcctgtt tttgtcgtcg accggaggac gaacgggtca
     1141 ccctccggtc cctccgccat gtcggccagg accggcccgc cgtcgtcagg agagtttcac
     1201 ccagaccgac ttggtctcga gataggcgtc cagtccctcg gcgctcagct cgcgccccca
     1261 gcccgattct ttgtaaccgc cgaagggcat ggcggcgtcg agcatcgaat agcagttgat
     1321 ccagacattg cccgtctcga ttcgcgcggc cagcctatgc gcggtgttga tgttggtcgt
     1381 gtagatgccg ccgcctaaac catagttcgt tgcattggcg agcgcgggca cttcttccag
     1441 atcatcgaag ggggtcgcga ccagcaccgg cccgaaaatc tcctcctgaa cgatcttcat
     1501 gttggcgtgg acgttggcga agacggtggg cgacacgaag aagcccttgc cctcgatcgg
     1561 ctggcccccc gacacgatct ccgcgccttc ggaaagaccg gtttggatat agccagtcac
     1621 gcggtcgcgt tgcttggccg acacgacggg gccgaggacc gtgtcctctt ccaggccgtt
     1681 gcccaccttg agactctgga gcacccggcc gatgcccgcc acgactttgt cgaacgattt
     1741 gcgctgcacg aacaggcggg agcccgcgaa gcagacctga cccgtattgg cggcgatcgc
     1801 catggccgcg ccaggaatgg cgatgtccat gtcggcatcg tcgaacacca cgcagggcga
     1861 cttgccgccg agttccagcg tcacacgctt gaggttgccg gcagacgcac ggacgagctc
     1921 tttgccgacg gcggtcgatc cggtgaagct gatcttgttg atgccggggt gattggccat
     1981 ggccgcgccc gcgcccgctc cgaagcccgg cacgacattg agcacgccgg ccgggacgcc
     2041 tgcctcgagc gccagctcgc ccagccgcag cgctgtcagc ggggtgatct cggacggctt
     2101 ctgcacgatg ctgcagcccg aggcgagtgc aggagcgacc ttgatgaaga aggtgccgat
     2161 cgggccgttc cagggcgtga tgaggccgac gacgccgatc ggttccttac gcgtatagac
     2221 atggaactcg ccgggcgccg atacagcgct ggaggctgtc tggccgtaga tgcgcgtcac
     2281 agagcctgcg aaccagcgca gccatttggc gccgccatgg acgctgttcc gtgcaaaggc
     2341 gcgcggcatg ccgttgttca ttgtttccag cagcgacagc tcgtcgccat tttcctccac
     2401 caggtcggcg aagcgcagca tgatgcgcgc ccgctccgac gcgggcaggc tgcgccatac
     2461 gccgctctcg aaactcttgc gcgccgcgga aacggccagt gcaatgtccg ctgcgtcgcc
     2521 ctcagccacg gtggcgatca cttcctcggt ggcggggttc acgacctcga aggtcttgcc
     2581 cgactgggcc gcaatatgct tgccgtcgat cagcagcagc ttggtccctt gcaggaaagc
     2641 aggggcttcg gaacgttcca gtaatgtcgc catgatttga actcctctat gttccgtaga
     2701 tctcgcagag gcccgccgcc tgcatgcgga ccagcgctcc gccctcgcgc agggcgcgca
     2761 gttcgacctc gacgatcctc cagcggctcg ttggccgcga agacgactgc ggcgcgcact
     2821 ttcatgcccg ccgcgctcat gcggtctgcg gctcttccac cgaaccggcg agctgctgga
     2881 cgagttgcgc cttgacgatc ttgcccatgg cattgcgcgg catggcgggc agcacgatcc
     2941 agtcgacggg cgccttgtag gcggcaaggt tggtggtgca caggccctgc agccgctcga
     3001 tcagcgcctg ccgctcgctt tccggtccgc tttcggtctc gatgaaggca gccacggtct
     3061 ggccgagccg cgcatgcggc ttgcccacga ccgcgcagtc gcgcacctgc gggtcgagcc
     3121 gtagcacacg ctcgacctcc gccggataga cgttcgcgcc gccgcgcagg atgaggtccg
     3181 atccgcggtc gcgaatgtgg aattcgccgc tctcgtcttg cacgcccacg tcgccggtgt
     3241 ggacccagcc gtcctgcagc agcttggcgg tcgactcggg ccgtttccag tagccgaggg
     3301 tcggcttgta gatgccggcc catggccccg tgtcggtcgc ccggatcgcg atttcgccca
     3361 cctcgccgcg gggcgcttcg cggccctctt tgtcgaggat ggcgaggtga aggtggggca
     3421 gcggccggcc gatgacaccc gtctcttccg ggctgttctc atgcgtcttg gcaacaccgt
     3481 tcggcccttc ggtaaggccg tagcccaccg tcatgcgccg gccgaagcgc tccttataga
     3541 gctggggcag cgattggggc accgtcgatc cgccgacgac cagccagcgc agcgagcgga
     3601 gctgctcttg atcgatatcc ggccgtgtca gcagatcctg gaccagcgtc ggcacgagcg
     3661 agatgttcgc gatcttctcc tgaccgatcc attccgccac gccctgcgcg tcgatacggt
     3721 ccagattcac atgcacggcg ccggtcgaaa gggcggcgag tggtcccagg atcatgacgt
     3781 tcaggatggt gagggggagg gcggtacccc gcaccgtctc cggcacgccg tcgccgctat
     3841 gctcgaccga gatggtggcg gtgaccttca tattgtgctg gctgtggacc acgcccttgg
     3901 gaaagccgg
//
LOCUS       DATNBV010000121         7866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4222954,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000121 DATNBV010000000
VERSION     DATNBV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4222954"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /gene="miaA"
                     /locus_tag="VN627_02935"
     CDS             complement(<1..837)
                     /gene="miaA"
                     /locus_tag="VN627_02935"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXD02242.1"
                     /translation="MSTDESLVREGDLPPVALIAGPTASGKSDLAVRLALALAARGRK
                     AVVINADSAQVYADLAVLSARPSPAEMQGMPHRLYGAWDGARACSAADWAEAARREIA
                     TAHDADAVPILVGGTGLYLRTLIDGIAPVPPIDPAVRDAVRALPVAEAHAALALEDPD
                     RAALLHPSDTTRVARALEVVRSTGRPLAHWQAERSGGIGAAVTLHPAILLPDRQWLYE
                     RCDRRFAQMLTGGAIEEVQTLLARGLDLDLPVMRAIGVPEVAEFLGGRASVAMAQERG
                     AQA"
     gene            830..1714
                     /gene="serB"
                     /locus_tag="VN627_02940"
     CDS             830..1714
                     /gene="serB"
                     /locus_tag="VN627_02940"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HXD02243.1"
                     /translation="MLIARLIADTSTLAENLDRARALLEGQGIAIAMAETVLSGEDAI
                     ELQLPHGSPAALRAVLDECFSPSDMLISDRPIEVPHLFVSDMDSTMISAECIDELADF
                     AGIKDQIAAITERAMQGELDFASALTERVALLKGLGEEAIGQCLAERIAPMPGARTLV
                     STLKARGCFTVLVTGGFHQFADPVAEQLGFERVVANRLAVHGGKLTGGLVGGITDSGV
                     KRTVLLEEMARLGADAVSLATGDGANDIPMIEAATYGVAYYAKPKARAAADGWVDRGD
                     LTSVLTLLGIPREEWIWE"
     gene            complement(1788..3977)
                     /gene="purL"
                     /locus_tag="VN627_02945"
     CDS             complement(1788..3977)
                     /gene="purL"
                     /locus_tag="VN627_02945"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HXD02244.1"
                     /translation="MSDTASQITPDVVAAHGLSEEEYARVLTALGREPNMVELGIFSV
                     MWSEHCSYKSSKLHLMKLPTKAPWVICGPGENAGVIDIGDGQAAIFKMESHNHPSYIE
                     PYQGAATGVGGILRDVFTMGARPVANMNALRFGRPDHPKMKHLVQGVVAGIGGYGNCV
                     GVPTVGGETNFHKAYDGNILVNAMTVGVAQTDKIFYCAATGLGNPIVYVGSKTGRDGI
                     HGATMASADFDDKSEEKRPTVQVGDPFTEKLLIEACLELMATDAIVAIQDMGAAGLTS
                     SSVEMASKGGAGIRLNMNAVPCREEGMTPYEMMLSESQERMLMVLQPGKEPMAEAIFR
                     KWELDFAVIGEVTDTGHMVLEFDGEVVCDIPLGPLADDAPLYDRPHVPTPKPAPLADV
                     PASENIAADLVKLIGCPDLASRRWIWEQYDSQVGADTIQKSGGDAALVRVHGSGKALA
                     ISTDCTPRYCYADPFEGGKQAVAETYRNICAVGAVPLAITNCLNFANPQRPEIMGQIV
                     GCLEGMGEACRALDYPIVSGNVSLYNESKATGGGSAILPTPAIGGVGLTTDWTKNATI
                     AFKTEGDTVWLVGDLGTHLGQSLWLRELHGREEGPPPPVDLKAERANGFAVRGWIEQG
                     LATAVHDLSDGGLLVGLAEMAMAGGIGATLDVSLDTAQAFGEDQARYIVTTKAGVALD
                     GARKIGTVGGDSLAGVKVSVLKAENERFFRDWMETGAALDVTDISGN"
     gene            4162..4638
                     /locus_tag="VN627_02950"
     CDS             4162..4638
                     /locus_tag="VN627_02950"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HXD02245.1"
                     /translation="MLKKIVVAAAMASALAATAAMADEVRSDDPSSLVRAMQDSGYRA
                     ELTKDNSGDPMIKSSASGNDFTILFYGCKSNRNCTTVQFFAGFKDPDNGSLSAMNKWN
                     TDNRFGRAYQAESGSARIEYDLDLDTGGMSAALFKDNLEVFAALVDRYRKYVYNLK"
     gene            4722..4988
                     /locus_tag="VN627_02955"
     CDS             4722..4988
                     /locus_tag="VN627_02955"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXD02246.1"
                     /translation="MTQSSSSLDPAIAALSFEDALGALEQVVRQLESGEVPLDDSITL
                     YERGEALRRHCQARLDAAQARIERIVAGADGQATSVQPFDDPDR"
     gene            5008..5898
                     /locus_tag="VN627_02960"
     CDS             5008..5898
                     /locus_tag="VN627_02960"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HXD02247.1"
                     /translation="MSDILKQALAAIQADVDAGFDALLPLPGDARDRLVEAMRYAAIG
                     GGKRIRPLLLTATAEMLQVDRAMAVRASLAIEAIHVYSLIHDDLPCMDDDALRHGKAT
                     VHRAYDEATAVLAGDALHAFAFEVLADPALTGDPFTRAELVQCLATASGLSGMAGGQM
                     MDIAAETSQFDLPTVTRLQQLKTGALLGAAVEMGAILGRLPTEGRTHLRGYARDIGLA
                     FQIVDDLLDHEGDEAAAGKALRKDAAAGKQTFVSLLGPERAREQARLLVDQAVAHVVQ
                     HGAEADTLRALARYIIERDR"
     gene            5900..6427
                     /gene="coaD"
                     /locus_tag="VN627_02965"
     CDS             5900..6427
                     /gene="coaD"
                     /locus_tag="VN627_02965"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HXD02248.1"
                     /translation="MPNAGTATRIGVYPGTFDPITLGHIDIIRRGAKLVDKLIIGVTT
                     NPSKNPMFTPAERMATVKREVAAMGITNVEVVGFNALLMKFAEVQGASVIIRGLRAVA
                     DFEYEYQMAGMNQQLNPGIETIFLMADVSLQPIASKLVKEIALFGGEIARFVSPAVRD
                     QVLERVGQLGRLGEY"
     gene            6551..7294
                     /locus_tag="VN627_02970"
     CDS             6551..7294
                     /locus_tag="VN627_02970"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXD02249.1"
                     /translation="MKRRSLLSALPLAIALPFALAAAPAFAKKAPKLAAAPPTQLSAV
                     VDTDPTHDPDDVLYLDLSNGGRVAIRLVPKWAPNHVERIKTLTKQGFYNGIIFHRVID
                     GFMAQTGDPTGTGQGGSPLPDLKAEFNALPHVRGTVSMARTDQPDSANSQFFIVFYPR
                     FALDRKYTNFGRVIGGMQYVDAIERGEPPEHPTKILQASLGSENKPQVMPAPEAPPPS
                     LTAPQPVTSLAPAPVKPAAAPKPKLRKRH"
     gene            7380..>7866
                     /locus_tag="VN627_02975"
     CDS             7380..>7866
                     /locus_tag="VN627_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXD02250.1"
                     /translation="MIEIGNLAVARVLEAEGPPTPLTLLGPDPALVERHRSWLYPRFI
                     DAADESFRLNFQTWVVDLDGFVVVIDPCNGNGRQRDMPMFNQLDTPFLERFAATGFAP
                     ERVDAVFCSHLHCDHCGWNTQARGGRWVPTFPNARYYFVAAEAQRWDTRRADYRKVDF
                     EV"
BASE COUNT         1269 a         2693 c         2602 g         1302 t
ORIGIN      
        1 cgcctgggcc ccacgctcct gtgccatggc gaccgaggcc ctgccgccga ggaactctgc
       61 cacttccggc acgccgatcg cccgcatcac cggcaggtcg aggtcgagcc cgcgcgcgag
      121 cagcgtctgc acttcctcga tcgcgccgcc ggtcagcatc tgcgcaaagc ggcggtcgca
      181 gcgctcgtaa agccactgcc gatcgggcag gaggatcgcg ggatgcaggg tcaccgcggc
      241 gccgatccca ccgctgcgct cggcctgcca gtgggcgagg gggcgcccgg tcgagcgcac
      301 cacctccagc gcgcgggcca cgcgggtcgt gtccgacggg tggagcaggg cggcccggtc
      361 ggggtcctcg agcgccagcg cggcatgggc ctcagccacg ggcaaggcgc ggacggcgtc
      421 gcgcacggcg ggatcgatcg gcggaaccgg cgcgatgccg tcgatcaggg tgcgcaggta
      481 aagcccggtc ccgccgacga ggatcggcac cgcatcggca tcatgagctg tcgcgatctc
      541 ccgccgcgcc gcctcggccc agtcggcggc agagcaggcc cgcgcgccgt cccaggcgcc
      601 atatagccga tggggcatgc cctgcatctc tgccgggctt ggtcgcgcgc tgagcacggc
      661 gaggtcggca tagacctggg cagagtcggc attgatcacc acggccttgc ggccccgcgc
      721 agcaagggcc agtgccagcc gcacggcgag atcgctcttg ccgctcgcgg tcggccctgc
      781 aatgagcgcg accggcggca ggtcgccctc acggacaagg gattcatcgg tgctcatcgc
      841 gcggctgata gcagacactt cgacacttgc ggaaaatctc gatcgggccc gcgccctgct
      901 ggaaggccag ggcattgcca tcgccatggc cgagactgtg ttgagcggcg aggatgcgat
      961 cgagctccaa ttgccccacg gcagcccggc ggcgctgcgc gccgtgctcg acgagtgctt
     1021 cagcccatcg gacatgctga tcagcgatcg cccgatcgag gtcccgcacc tgttcgtgtc
     1081 cgacatggat tcgaccatga tctcggccga atgcatcgac gagttggccg atttcgccgg
     1141 tatcaaagac cagatcgccg cgatcaccga acgcgccatg caaggcgagc tggacttcgc
     1201 ctcggcgctg acggagcggg tcgcgctcct caagggcctg ggcgaagaag ccatcggcca
     1261 gtgcctcgcc gaacgcatcg cccccatgcc gggcgcgcgc accctggttt ccaccctcaa
     1321 ggcgcgcggc tgctttaccg tgctggtgac cggcggcttt caccagttcg ccgatcccgt
     1381 ggccgagcag ctcggcttcg agcgcgtcgt ggcgaaccgg ctggctgtcc acggcggcaa
     1441 gctcaccggc ggcctcgtcg gaggaattac cgacagcggg gtcaagcgca ccgtgctgct
     1501 ggaggaaatg gcccgcctcg gcgcggatgc cgtcagcctc gccaccggcg acggcgccaa
     1561 cgacatccca atgatcgagg ccgcgaccta tggcgtcgcc tactacgcca agcccaaggc
     1621 ccgcgcggcg gccgatggtt gggtggaccg gggggacctg acttcggtcc tgaccctgct
     1681 gggtattccg cgcgaggaat ggatctggga ataagaggag aggcccctcc gtcagtccgg
     1741 cgggatgcca cctccccatc gccgcgcgac ggggaggatc tgtcgcctca attgccgctg
     1801 atatccgtca catccagcgc cgcgccggtt tccatccagt cgcggaagaa gcgttcgttc
     1861 tcggccttga gcacgctgac cttgaccccg gcaaggctgt cgcccccaac cgtgccgatc
     1921 ttccgcgcgc cgtccagcgc gacgccggcc ttggtggtca cgatatagcg cgcctggtcc
     1981 tcgccgaagg cttgggccgt gtcgagcgac acgtcgagcg tggcgccgat cccgccagcc
     2041 atggccattt cggccaggcc taccagcagg ccgccgtcgg acaggtcgtg gaccgccgtg
     2101 gccagcccct gctcgatcca cccgcgcacg gcaaagccat tggcgcgttc agccttgagg
     2161 tcgaccggcg gcggcgggcc ttcctcgcgg ccatgcagtt cgcgcagcca cagtgactgg
     2221 cccagatggg tgccgagatc gcccaccagc caaaccgtgt cgccctcggt cttgaaggcg
     2281 attgtcgcat tcttcgtcca gtccgtggtc aggcccacgc cgccgatggc cggagtcggc
     2341 aggatcgccg agccaccgcc ggtggccttg ctctcgttgt agagcgagac gttgcccgaa
     2401 acgatcggat agtcgagcgc gcggcaggcc tcgcccatgc cttcgaggca gccgacgatc
     2461 tggcccatga tttcagggcg ctgcgggttg gcgaagttga ggcagttggt gatggcgagc
     2521 ggcacggcgc ccaccgcgca gatgttgcgg taggtctcgg ccacggcctg cttgccgccc
     2581 tcgaacggat cggcataaca atagcgcggg gtgcagtcgg tgctgatcgc cagcgccttg
     2641 ccgctgccgt ggacgcgcac cagcgccgcg tcgccgccgc tcttctggat cgtgtcggcg
     2701 ccgacctggc tgtcgtactg ctcccagatc cagcgccggc tggcgaggtc cgggcagccg
     2761 atcagcttga cgagatcggc cgcgatgttc tcgctcgccg gcacatcggc cagcggcgcg
     2821 ggcttgggcg tcggcacgtg gggccggtca taaagcgggg cgtcgtcggc cagaggccca
     2881 agcggaatgt cgcagaccac ctcgccgtcg aattcgagca ccatgtggcc ggtgtcggtt
     2941 acttcgccga taaccgcgaa gtcgagctcc cacttgcgga agatcgcctc ggccatcggc
     3001 tccttgccgg gctgaagtac catgagcatg cgctcctggc tctcgctcag catcatctcg
     3061 tagggggtca tgccttcctc gcggcaaggc acggcattca tgttgagccg gatgcccgct
     3121 ccgcccttgc tggccatttc gaccgagctt gaggtgagcc ccgccgcgcc catgtcctgg
     3181 atggcgacga tggcatcggt cgccatcagt tcgaggcagg cctcgatcag cagcttctcg
     3241 gtgaacggat cgcccacctg cacggtcggg cgcttttcct cgctcttgtc gtcgaaatcg
     3301 gcgctcgcca tggttgcgcc gtggatgccg tcgcgcccgg tcttcgagcc gacgtagacg
     3361 atgggattgc ccagccccgt cgccgcacag tagaagatct tgtcggtctg ggcgacgccc
     3421 acggtcatcg cgttgacgag gatgttgccg tcataggcct tgtggaagtt ggtctccccg
     3481 ccgaccgtgg gtacgcccac gcagttgccg tagccgccga tacccgcgac cacgccctgg
     3541 acaaggtgct tcatcttggg atggtccggc cggccaaagc gcagggcgtt catgttggcc
     3601 acgggcctgg cgcccatggt gaaaacgtcg cgcaggatgc cgcccacgcc cgtcgccgcg
     3661 ccctggtagg gttcgatgta gctcgggtgg ttgtggctct ccatcttgaa gatggcggcc
     3721 tgaccatcgc cgatatcgat cacgccggca ttctcgccag gcccgcagat cacccagggg
     3781 gctttcgtgg gcagcttcat caggtgcagc ttgctcgact tgtaggagca atgctccgac
     3841 cacatgaccg aaaagatgcc cagctcgacc atgttcggct cgcggccgag cgcggtcaaa
     3901 acgcgggcat attcttcctc ggacagcccg tgggcggcga cgacgtctgg ggtgatctgg
     3961 ctggcggtat cggacatggc gctgccctta gcgccggcaa gcgcggctgg ccagactcga
     4021 cgatgcccca atcccgcact tgtgccctgt cgctggcatt gcgggaaggt gtttactcgc
     4081 gggctttgcc cggtatcaat gatcggcggt acttgccgca ctagggatcc ggcaagtcat
     4141 gcgacggaac aagggggctg tatgttgaag aagattgtag tggctgcggc aatggcaagc
     4201 gcgctggcgg caacggctgc catggccgat gaggtccggt cggacgatcc gtcctccctg
     4261 gtccgggcca tgcaggactc cggctaccgg gccgagctga ccaaggacaa ttcgggcgat
     4321 ccgatgatca agagctcggc ctcgggcaac gatttcacga tcctgttcta tggttgcaag
     4381 agcaatcgca actgcaccac ggtccagttc ttcgccgggt tcaaggaccc ggacaacggc
     4441 tcgctctcgg ccatgaacaa atggaacacc gacaatcgct tcggccgggc ctaccaggct
     4501 gagagcggga gcgcccggat cgaatatgac ctcgatctcg acaccggcgg gatgagcgcg
     4561 gcgctgttca aggacaatct cgaagtcttc gcggcgctgg tcgatcgcta ccggaaatac
     4621 gtctacaacc tcaagtaagg ctgcggggca gcggggggag gggacatgtc cgcccgggca
     4681 tgcttgaccc tcgccccgcc cggggccaaa gcaggcggat catgacccag agctcttcca
     4741 gcctcgatcc cgccatcgcc gcgctttcgt tcgaggatgc gctcggcgcg ctcgaacagg
     4801 tggtgcgcca actggaaagc ggcgaggtcc cgcttgacga ttcgatcact ctctatgagc
     4861 gcggcgaggc gctgcggcgc cattgccagg cgcgcctcga tgccgcccag gcccggatcg
     4921 agcggatcgt ggccggggcg gacggccagg cgaccagcgt ccagccgttc gacgatccgg
     4981 accgctgagc ggctcgggga ggggaacgtg agcgatatcc tgaagcaggc cctcgcggcg
     5041 atccaggccg atgtcgacgc cgggttcgac gcgctcctgc cgttgcccgg cgatgcgcgc
     5101 gaccggctgg tcgaggccat gcgctatgcc gcgatcggcg ggggcaagcg cattcgcccc
     5161 ctgctgctga ccgccacggc cgagatgctg caggtggacc gcgccatggc cgtgcgggcc
     5221 agcctcgcga tcgaggcaat ccacgtctat tcactgatcc acgacgatct gccgtgcatg
     5281 gacgacgatg ccttgcgcca cggcaaggcc accgtccacc gcgcctatga tgaggcgacc
     5341 gccgtgctgg ccggcgatgc gctccatgcc ttcgccttcg aggttctcgc cgatccggcg
     5401 ctgaccggcg atcctttcac ccgcgccgaa ctggtccagt gccttgccac tgccagcggg
     5461 ctgtccggca tggccggcgg ccagatgatg gatatcgccg cggaaaccag ccagttcgac
     5521 ctgcccaccg tcacccgcct gcagcagctg aagaccggcg ccctgctcgg cgcggcggtc
     5581 gaaatgggcg cgattcttgg ccgcctgccg acggaagggc gcacgcacct gcgcggctat
     5641 gcccgggata tcggtctcgc cttccagatc gtcgacgacc tgctcgacca tgagggtgac
     5701 gaggccgccg ccggcaaggc cctgcgcaag gacgcggcgg ccggcaagca gaccttcgtc
     5761 tccctgctcg gccccgagcg ggcgcgcgag caggcccgcc tgctggtcga ccaggccgtt
     5821 gcccacgtcg tccagcacgg tgccgaggcc gacaccctgc gggcactggc gcgctacatc
     5881 atcgagaggg atcgctgaaa tgccaaatgc cggcactgcc acgaggatcg gcgtctatcc
     5941 ggggactttc gacccgatca cgcttgggca catcgatatc atccgccggg gcgccaagct
     6001 ggtggacaag ctgatcatcg gggtgacgac gaacccgtcg aagaacccga tgttcacgcc
     6061 cgccgaacgc atggccacgg tgaagcgcga agtcgcggcg atgggcatta ccaatgtcga
     6121 agtggtcggc ttcaacgcgc tgctgatgaa gttcgccgag gtgcaggggg ccagcgtcat
     6181 catccgcggc ctgcgcgccg tggccgactt cgaatacgaa taccagatgg ccggcatgaa
     6241 ccagcagctc aatccgggga tcgagacgat cttcctgatg gccgacgtgt cgctccagcc
     6301 gatcgcttcc aagctggtca aggagatcgc cctgttcggc ggcgagattg cgcgcttcgt
     6361 cagccccgcc gtgcgcgacc aggtgctgga gcgggtggga cagctcggcc ggctcgggga
     6421 atattgatcg gggcaaatcc ggcgggagcg gtcgtgccgt tcattgccat gacagtcggg
     6481 cgcggctaca cgcgccacgg tatttcgtct caccggatct tcggttgggc cgtttcgcga
     6541 gagtatcctg atgaagcgtc gtagccttct ctctgccctg ccgctcgcga ttgcgctccc
     6601 gtttgcgctc gctgccgcgc cggcctttgc caagaaggcg ccgaagctgg ccgccgcccc
     6661 gccgacccag ctgagcgcgg tggtggatac cgatcccacc cacgatcccg atgacgtgct
     6721 ctacctcgat ctgtcgaacg gcgggcgcgt cgccatccgg ctggtgccca agtgggcgcc
     6781 caatcatgtc gaacggatca agaccctgac gaagcagggc ttctacaacg ggatcatctt
     6841 ccaccgcgtg atcgacggtt tcatggcgca gaccggcgat ccgaccggga cggggcaggg
     6901 cggctcgccg ctgcctgacc tcaaggccga attcaatgcg ctgccccacg tgcgcggcac
     6961 tgtctcgatg gcgcggaccg accagcccga cagcgccaac agccagttct tcatcgtctt
     7021 ctatccgcgc ttcgcgcttg accggaagta caccaatttc ggccgggtga tcggcgggat
     7081 gcaatatgtc gatgcgatcg aacgcggcga gccgcccgag catccgacca aaattctcca
     7141 ggcttcgctg gggagcgaga acaagcccca ggtcatgccc gcgcccgaag cgccgccgcc
     7201 aagcctgacg gcccctcagc cggtgaccag cctcgctccg gcacccgtca agccggcagc
     7261 cgcgcccaag ccgaagctcc gcaagcggca ttgagctagg gtcctgaccc taacggcggg
     7321 gcttgtcgcc cgccctgaga cgtgctccca tccgcccgtg tcggagggag ccgcaggcga
     7381 tgatagaaat tggcaatctg gccgtagccc gcgtgcttga ggccgagggg ccgcccacgc
     7441 cgctcacctt gctcggcccc gatccagcct tggtggaacg ccaccgcagc tggctctacc
     7501 cgcgcttcat cgacgcggcc gatgaaagct tccgcctcaa tttccagacc tgggtggtgg
     7561 acctggacgg gttcgtggtc gtcatcgatc cgtgcaacgg caatggccgc cagcgcgaca
     7621 tgccgatgtt caaccagttg gacacgccct ttctcgaacg cttcgccgca accggctttg
     7681 cgcctgagcg ggtcgacgcg gtgttctgct cgcacctcca ttgcgatcac tgcggatgga
     7741 acacccaggc gcgcggcggg cgctgggtcc ccacctttcc caatgcccgg tactacttcg
     7801 tcgccgccga ggcccaacgc tgggacaccc ggcgggccga ctaccggaag gtcgatttcg
     7861 aagtgg
//
LOCUS       DATNBV010000122         3276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3583700,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000122 DATNBV010000000
VERSION     DATNBV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3583700"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="VN627_02980"
     CDS             complement(<1..1770)
                     /locus_tag="VN627_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HXD02251.1"
                     /translation="MRFRAIIAALATIALAACGGGGSSTPSSAVTPTPTGTATAAASY
                     TAPAQEALTVGDVQKVLAQAAAQASADSSPATVAVVDRVGNVLAVMRMTGAPTLAHIP
                     EASDGSKQDVQGLDVPAEAAAIAKAITGAYLSSGGNAFSSRTASMIVQQHFPPAPTTV
                     GLESGPLFGVQFSQLPCSDLSTRLSEGLIGPKRSPLGLAADPGGFPLYKNGVVVGGVG
                     VMADGVYGLDPNLLDVDADKDEHIALAATLGFEAPEAIRADKIYVDGTQLRYSDATYA
                     GVTSTVAAATYTATVPTIAALVPVTGYFAGTIQPGTAYGTEASGVRPVTTAEFSNSNA
                     FLLSNGAGAARFPIRAGTDAAEVGTALAAAEVRTVLEQAFTVMSRARAQIRQPLDSRA
                     QVTISVVDTHGVPLGQVRSPDAPIFGIDVSLQKARTAAFMSNANAGADLLANPSSDVR
                     GFVPALRTFLGDPAALTGKFAFSDRAGGLLSRPYFPDGQVGQPNGPLSRPIAKWSPFS
                     TGLQSALVLTNLAQHLGYVTGASGTDTAHRCTFSPDVVSGQNRLQNGIQIFPGSVPLY
                     RGSQLVGAIGVSGDGIDQDDMISF"
     gene            complement(1770..3092)
                     /locus_tag="VN627_02985"
     CDS             complement(1770..3092)
                     /locus_tag="VN627_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HXD02252.1"
                     /translation="MLCLLGLATAAYASGGFANATFEGVASCAGATCHGRAEGNGKVI
                     RQDELAHWQDPATQGGAHSRAYAVLAGHRGQQIAASLGLGPATSAPACLGCHSTDAPA
                     ALRGERYLQTDGVGCESCHGAAGSWIASHYTMAGTHAANVAAGLVPLENPRTRANVCL
                     DCHFGSGKPGQFVTHRMMAAGHPRVSFELDLFSALQQHHDEDADYLQRKGRTDSVRLW
                     AVGQAEAVRRSLTLFAQPRLASQGVFPEFYFYDCHSCHRTIEDGAERKLTFEVNPARP
                     QPFGTPPYNDENMILLSAAAKALAPAEGARFDAAARAFHVAMGAGRAEAVAAGNRLAA
                     AAGNLSNALSSGNYGNDSAFRVIAAVAGSAISPRFTDYTGSVQAVMAVDTLLNALVRE
                     GRVTVGAAAGIRADINRAYAAVKGPNAYSPPAFRAALGSAARSVEALR"
BASE COUNT          510 a          996 c         1299 g          471 t
ORIGIN      
        1 gaacgaaatc atgtcgtcct ggtcgatgcc gtcacccgag acgccgatcg cgccgaccag
       61 ctggctgccg cgatagagcg ggaccgagcc ggggaagatc tggatgccgt tctgcaggcg
      121 gttctggccc gagaccacat cggggctgaa ggtgcagcgg tgggcggtgt cggtgccgct
      181 ggcgccggtg acatagccga ggtgctgggc caggttggtg aggaccaggg ccgattgcag
      241 gccggtggag aacgggctcc acttggcgat cgggcgcgac agcgggccgt tgggctggcc
      301 gacctggcca tcggggaaat agggccgcga gagcaggccg ccggcgcgat cggaaaaggc
      361 gaacttgccg gtgagcgcgg cgggatcgcc caggaaggtg cgcaaggcgg ggacgaagcc
      421 gcgcacgtcg ctgctggggt tcgccagcag gtcggcccca gcgttggcat tggacatgaa
      481 ggcggcggtg cgggccttct gcagcgagac gtcgatcccg aagatcggcg catcgggcga
      541 gcggacctgg ccgagcggca cgccatgggt atcgacgacc gagatcgtca cctgggcgcg
      601 gctgtcgagc ggctggcgga tctgagcgcg ggcgcggctc atcacggtga atgcctgttc
      661 gagcacggtg cgcacttcgg cggcggccag cgccgtgcca acttctgcgg catcggtgcc
      721 ggcgcggatc gggaagcgcg cggcccctgc cccgttgctc aggaggaagg cgttgctgtt
      781 cgagaattcg gccgtggtga ccgggcgcac gcccgaggcc tcggtgccat aggcggtgcc
      841 gggctggatc gtgccggcga agtagccggt gaccgggacc agcgcggcga tggtggggac
      901 cgtggccgtg taggtggcgg cggcgaccgt gctggtgacg ccggcatagg tggcatcgga
      961 atagcgcagc tgggtgccgt cgacatagat cttgtcggcc cggatcgcct cgggcgcctc
     1021 gaatccgagg gtggccgcaa gcgcgatatg ctcgtccttg tcggcatcga cgtcgagcag
     1081 gttgggatcg aggccataga ccccgtcggc catgacgccg accccgccga cgacgacccc
     1141 gttcttgtag agcgggaagc cgcccggatc agcggcaagg ccgagcggcg agcgcttggg
     1201 cccgatcagg ccttcggaca ggcgggtgga gaggtccgaa cagggcagct ggctgaactg
     1261 gaccccgaac agcgggccgc tttccaggcc gaccgtggtc ggcgcggggg ggaagtgctg
     1321 ctggacgatc atggaggcgg tgcggctgga gaaggcattg ccgccgctgg agagataggc
     1381 cccggtgatc gccttggcga tggcggcagc ctccgcgggt acatcaaggc cctgcacgtc
     1441 ctgcttcgag ccgtcgctcg cctcggggat gtgggcgagc gtgggagcgc cggtcatccg
     1501 catgaccgca aggacattgc cgacccggtc aaccacggcg accgtggcgg ggctgctatc
     1561 ggccgaggcc tgggccgcgg cctgggcgag gaccttctgg acatcgccga cggtgagcgc
     1621 ttcctgggcc ggggcggtat aggacgcggc ggcggtggcc gtgcccgttg gcgtgggggt
     1681 gacggcggag gacggggtgg aactgccccc gccaccgcag gcggcgagcg cgatggtggc
     1741 aagagcggca atgatggcgc gaaaccgcat cagcgcaggg cctccaccga gcgtgcagcc
     1801 gagccgagcg ccgcgcggaa ggcaggcggg ctgtaggcat tggggccttt caccgccgca
     1861 taggcgcggt tgatgtcggc acggatgccg gcggccgcgc cgaccgtgac ccggccttcg
     1921 cggaccaggg cattgagcag ggtatcgacg gccatcaccg cctggaccga gccggtatag
     1981 tccgtgaagc gcggactgat cgcgctaccc gcgactgcgg cgatgacgcg gaaggcgctg
     2041 tcatttccgt aattgccgga ggaaagcgcg ttcgagagat tgcccgccgc ggcggcgagg
     2101 cgattgccgg cggcgacggc ttcggcgcgc cctgccccca tggcgacatg gaaggcgcgc
     2161 gcggcggcat cgaagcgggc gccttcggcc ggggcgagcg ccttggcggc ggccgagagc
     2221 aggatcatgt tctcgtcatt atagggcggc gtgccgaagg gctgcgggcg ggccgggttg
     2281 acctcgaaag tgagcttgcg ctcggccccg tcctcgatcg tgcggtggca cgagtggcag
     2341 tcgtagaagt agaattcggg gaagacgccc tgcgaggcaa ggcgcggctg ggcgaagagg
     2401 gtgagcgagc ggcgcacggc ctcggcctgg ccgacggccc agagacgcac gctgtccgtg
     2461 cggcccttgc gctggagata gtccgcatcc tcgtcgtggt gctgctggag ggcgctgaac
     2521 aggtcgagct cgaacgagac gcgcgggtgg ccggcggcca tcatccggtg cgtgacgaac
     2581 tggccgggct tgcccgagcc gaagtggcaa tcgaggcaga cattggcgcg ggtgcgcggg
     2641 ttttccaggg gaacgaggcc cgcggcgacg ttggccgcgt gggtgccggc catggtgtag
     2701 tggctggcga tccacgagcc ggcggcgcca tggcaggact cgcagccgac gccgtctgtc
     2761 tgcaaatagc gctcaccccg caaggcggcg ggggcatcgg tggaatggca gccgaggcag
     2821 gccggcgcgg aggtggcggg gccgaggccg agcgaggcgg cgatctgctg gccacgatgg
     2881 ccggcgagga ccgcataggc ccggctgtgg gccccgccct gcgtggccgg atcctgccag
     2941 tgcgccaatt cgtcctggcg gatgaccttg ccgttgcctt cggcgcggcc atggcaggtg
     3001 gctccggcgc aggaggcgac gccctcaaag gtggcattgg cgaagccgcc ggaggcatag
     3061 gcggcggtgg ccaggccgag caggcacagc atggtgacca gcagcgcccg ggcgcgcctt
     3121 ccagccgaag atggaatcac ctgcatggcc cccttgccaa tgtccctgtc cttctatcgc
     3181 cggttagtgt tgctgccaca agtcatcgtt gcccacgcca gtaacgggtt cgtcaattag
     3241 tggtggagaa tcaaacaagg gcatgtcgcc aaagag
//
LOCUS       DATNBV010000123         2228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3586201,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000123 DATNBV010000000
VERSION     DATNBV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3586201"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..454)
                     /gene="trxA"
                     /locus_tag="VN627_02990"
     CDS             complement(134..454)
                     /gene="trxA"
                     /locus_tag="VN627_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXD02253.1"
                     /translation="MSTKAVTDASFDAEVLQSGKPVLVDFWADWCGPCKMIGPSLEEI
                     SEELADQVTIAKVDIMENTETASRFGVQSIPLLILFKDGQPVAQKLGAAPKSQLKSWI
                     EGSL"
     gene            complement(545..>2228)
                     /locus_tag="VN627_02995"
     CDS             complement(545..>2228)
                     /locus_tag="VN627_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXD02254.1"
                     /translation="IMILVRKRKELAGLIVARLHARGVPVAGVDRLRLGAPLAVQDLI
                     SALRIALQPLDDLTLSGLLVSPLLGWTQEQLLEHGWREKGVALWDHLRQSRDPLVTAT
                     VEQLVQLLRQADYAPPAALLHWLLVGPWQGRRKLVARLGREANDPIDELLNAAHAYAA
                     GDVPSLAGFLRWFDAGDGELKREAESGGNLVRVMTVHGSKGLQAPIVILADAADDPDR
                     GMGVALGLADPRPDGAAHLYHTPRVLPLPGLRKEEKLGRIAQAEAEAKAAEREEHWRL
                     LYVAMTRAEEALFVTGSLGARETAPAGDSWYARIAEVMPEESWETNGLWGALAVHGEV
                     PPPAPPRAAETQLLPLPAARPRWLTSPVGEEPVPPRPLAPSSLGEDDSADPPHPPTGD
                     GLAALRGTLIHKLLERLPEVAPAARAEAADLWLARNAAALPEPARAEMAAAALKVLAV
                     GEWADLFGPGALAEVPIAAIVGGRVIAGTIDRLLVTPETLRLVDFKTARRPPAGLGEV
                     PVAILRQMAAYAAALEVTFPGRRAEVALLYTHEPRLIEIPADVLAMHKPDFA"
BASE COUNT          362 a          807 c          724 g          335 t
ORIGIN      
        1 accggcatct tgagcccggc ctgctcggag cagcaagccc ggcgctatgg gatgaactgg
       61 ccgagcggat ggcgtcgctc tgacctgaac ggtcgcgcaa gcctgcaagc gcaggtcagc
      121 gcaccccttc caatcacagc gacccttcga tccacgactt cagctggctc ttcggcgccg
      181 cacccagctt ctgggcaacc ggctggccgt ccttgaacag gatcagcagc gggatcgact
      241 ggacgccgaa gcggctcgcc gtctcggtgt tctccatgat gtcgaccttg gcgatggtca
      301 cctgatcggc cagttcctcg gagatttcct cgaggctcgg cccgatcatc ttgcacggcc
      361 cgcaccagtc ggcccagaaa tcgacgagga cgggcttgcc cgactggagg acttcggcat
      421 cgaagctggc gtcggtgacg gccttggtgg acattcgaga atactccgtg agtgaggtgt
      481 tgcccggcaa tctaggcact ctgtggcagg gcgcaacacg gcagtcccga tagcaataat
      541 cgcctcaggc gaagtctggc ttgtgcatgg ccagcacgtc ggccgggatt tcgatcagcc
      601 gcggctcatg ggtatagagc agcgccacct cggccctgcg cccggggaag gtcacctcca
      661 gcgcggcggc ataggccgcc atctgccgaa ggatcgccac gggcacctcg ccgagcccgg
      721 ccggcgggcg ccgtgccgtc ttgaagtcga ccagccgcag cgtctcgggc gtaaccagca
      781 gccggtcgat cgtcccggca atcacgcgcc cgccgacgat cgccgcaatc ggcacctccg
      841 ccagcgcccc cgggccgaac aggtccgccc attcgcccac cgccaagacc ttcaacgctg
      901 ccgccgccat ctcggcccga gccggttccg gcaaagcggc ggcattccgg gccagccaca
      961 ggtccgccgc ctcggcacgg gccgcgggcg ccacttccgg caggcgctca agcagcttgt
     1021 gaatcagtgt tccgcgcagc gccgccaagc cgtcgccggt cggcggatgc ggcgggtcgg
     1081 cgctgtcgtc ctcgcccagc gatgaaggcg cgagcgggcg cggcggcacc ggctcctcgc
     1141 ccaccgggga tgtcagccag cgggggcggg ccgctggcag gggcagcaac tgtgtttcag
     1201 cggcacgcgg cggggccggt ggcggaactt cgccatgaac ggcaagagca ccccacaagc
     1261 cattggtctc ccaggattcc tcaggcatga cctcggcaat ccgggcatac caggaatcac
     1321 ctgccggtgc ggtttccctg gcgccgaggg agccagtgac gaacagcgct tcctcggccc
     1381 gcgtcatcgc cacatacagc agccgccagt gttcttcgcg ctctgccgcc ttggcctcgg
     1441 cctcggcctg ggcgatgcgc cccagcttct cctccttgcg cagcccaggc agaggcagca
     1501 cgcgcggggt gtgatagagg tgcgccgcgc catcggggcg cgggtcggcc agtcccagcg
     1561 ccacgcccat gccccggtcg ggatcgtccg ccgcatcggc gaggatcacg atcggcgcct
     1621 gcaagccctt ggaaccgtgg accgtcatca cccgcacaag gttgcccccg ctctccgcct
     1681 cgcgcttcag ttcaccgtcg ccggcatcga accagcgcag gaacccggcg aggctcggca
     1741 cgtcgcccgc ggcataggcg tgggcggcgt tgagcagttc gtcgatcgga tcgttggcct
     1801 cgcggcccag ccgggcgacc agcttgcggc gtccctgcca tggaccgacc agcagccagt
     1861 gcagcagtgc cgccggaggg gcatagtcgg cttgcctgag aagttgcacg agctgttcga
     1921 ccgtcgccgt caccagcgga tcgcgcgatt ggcgcaagtg gtcccacagc gccaccccct
     1981 tctcgcgcca gccgtgttcg agcaactgct cctgcgtcca ccccagcagt ggcgaaacca
     2041 gcagcccgga aagtgtaaga tcatcaagtg gttgcagggc aatcctcagc gccgatatga
     2101 ggtcctgcac cgccagcggc gcacccagcc gcaagcggtc gacgccggcc accggcacgc
     2161 cccgggcatg cagccgcgcc acgatcagcc cggccagttc cttgcgcttg cgcacaagga
     2221 tcatgata
//
LOCUS       DATNBV010000124         6361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2640454,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000124 DATNBV010000000
VERSION     DATNBV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2640454"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VN627_03000"
     CDS             <1..487
                     /locus_tag="VN627_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HXD02255.1"
                     /translation="SEVVLVDADFAKPSILSTLGLPGGPGLMDALADPAIDVADCVLG
                     TDIPGLWVLPAGNATASDTEFLASSRTRAVLDRLTEGAPHRMVIFDSPPALAASPAAE
                     LAKYVGQALVVARADRTGQSALEDAIALLSGCPNIQLLLNAVQFSPSGRRFGSYYGYT
                     G"
     gene            497..2230
                     /locus_tag="VN627_03005"
     CDS             497..2230
                     /locus_tag="VN627_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXD02256.1"
                     /translation="MDRIRTTAILAGALLAGIGFPAGAGAQSISYGKASPGAGEGGPS
                     ASSDDNAVEAPASSSGERRGRHGRLRLSPYIEAAQVISAELAPQHDVLTWSTLAVGVD
                     GGVAGRNTQASFSVRYERRFGYGKSDSGDTISGLARASVGLVPRVLSFDVGALATRTT
                     TDGGAAFPGSVDPNRSSRLWSAYAGPSLSTHAGDVAVNGAYRVGYTEVGSNSAVTNNG
                     VGGVVDLFDHSVTQMASLHAGLAPGHGLPIGVGLGGGYYHEDISNLDQQVKDLSLRAD
                     VAVPVSDTVSLVGGVGYENVRVSSRDALRDPLTNAPVVVGGRYVTDKSAPRQIAYDTS
                     GFIWDAGVTWRPSRRTAFEAHVGRRYGSTTYYGSFGWRASRRSTVNISVYDSISGLGG
                     SLNRALVALPTDFEVSRNPVTGDINGCVSALGNGSGSSPCLIGALGAVRSATFRSRGI
                     QGSWSVQASRFGFGFGAGYDRRKFIAAPGTILAAASGQTDESAWLSAWLSGRIDRDSS
                     FSTFAYVDWFQSGLDGSDGTAIGANASYSRALGHHLSANAALSIQGVQHSDGPDTWDA
                     SALLGMRYSFF"
     gene            2250..3437
                     /locus_tag="VN627_03010"
     CDS             2250..3437
                     /locus_tag="VN627_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXD02257.1"
                     /translation="MYEEFYGFTGRPFQLTPDAAFYFESLTHRKALSYLGYGLAQGEG
                     FIVITGEIGSGKSTLAAHLMATIDPERLTAAQVVTSALNGDEIIHVVAQSFGLVIDAN
                     DKATALAGIEGLLHAEARGGRRCLLVVDEAQNLSIEALEELRMLSNFQLGAQPLLQTL
                     LLGQPEFRDTLQSSPRLEQLRQRVIATHHLDAMDADEVQPYVEHRLRLVGWNGRPSIE
                     TPLYPALYEATGGVPRRINQIANRLFLMGCVEERDHFDMTLFERVMEEVATDTALAPA
                     APVAEAPVHPAEQAPAVAAAPIDAVPFAEMKEAFDERDVLISELQQAVIELANTLEGQ
                     PAPVLADSGIKADLAALNQRLSGMADRLGSLEARMVEQEASVKHTLAMLIEWLESDQK
                     AAA"
     gene            3460..4332
                     /locus_tag="VN627_03015"
     CDS             3460..4332
                     /locus_tag="VN627_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HXD02258.1"
                     /translation="MRVVNALSVDVEEWFQVGAFERVIARDEWDGLASRVEDNCARIL
                     DLFAGAGVSATFFTLGWIAERHPALVRRIHEAGHEIASHGWDHARVFTLGAKAFAADL
                     ERSRKALEDAAGAPVRGYRAPSFSIDARTPWAFAVLAEQGYAYSSSVAPVVHDHYGWR
                     EAPRFAFRPLADSALVELPVTTAEFGGRRLAAGGGGFFRVLPYGFTRAAIRQVNGLGQ
                     PAVFYFHPWEIDPAQPRVGHAPLRSRLRHYTGLARMAGKLERLTGEFAWGRVDAAVAG
                     LSSDTAPSPLALAA"
     gene            4329..5393
                     /locus_tag="VN627_03020"
     CDS             4329..5393
                     /locus_tag="VN627_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HXD02259.1"
                     /translation="MNAPFALPKLTVRLADAAEPAIAAFLADHPQATVFHGAAWLEAI
                     SAATGSDALALVAEARGEVRGYLPLSEIHSPVFGRLLASTGFAVGGGLLLAEGTDAAP
                     LLAAASELAQRRSCPAIELRGGVMPADGTGWHLKREAHCGFVAPLGESDEAQLLAIPR
                     KQRAEVRKGLEQDLAVTIGTGQRDRAAHHAVYSESVRNLGTPVFPRALFDCVLDRLGE
                     NADILTVWRGETPVASVLSLYHRSAVMPYWGGGTRAARALRANDRMYFELMAHARRRG
                     CTRFDFGRSKTGSGPYHFKRNWGFVPEPLTYANWTAPGCQPRDADPTSAKHRALIAAW
                     QLLPLAVANRLGPLIARGLG"
     gene            5397..>6361
                     /locus_tag="VN627_03025"
     CDS             5397..>6361
                     /locus_tag="VN627_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HXD02260.1"
                     /translation="MGEILFIAHRLPFPPDRGDKIRSHYLLRHLAGLGPVHVACFADD
                     EADAVHEGELAALAQSHCLVPRSRPLPLAGLAALATGRPVSLAAFADARLQAYVDRVA
                     ATRPLAAIVVFSGQMGQYVPAGWTGPLVVDFVDVDSAKFGAYAAAARGPMSWVHARED
                     RLLRAEEGRLAARADASLLVSEPEAELFRTRLGPDRQGRCKVVAIGNGIDVAAYDPEA
                     VAPEPRMAAMPGPRVIFTGQMDYPPNVAAVVRAASRIMPLVRRFLPAATFHVVGRKPA
                     AEVMALHGSNGCHVWGRVDDIRPWLRAADIALVPLEIARGVQNKVL"
BASE COUNT          890 a         2277 c         2133 g         1061 t
ORIGIN      
        1 aagcgaggtc gtcctcgtcg atgcggattt cgccaagccc tcgatcctgt ccaccctcgg
       61 cctccccggc ggccccgggc tgatggatgc gctggccgat ccggcgatcg acgtggccga
      121 ttgcgtgctc ggcaccgaca ttcccggctt gtgggtcctt ccggccggca atgcgaccgc
      181 gtcggatacc gaattcctcg cctcctcgcg cacccgcgcc gtgctggacc ggctgaccga
      241 gggcgcgccg caccgcatgg tgatcttcga cagcccgccc gcgctggccg cctcgccggc
      301 ggcggagctg gcgaaatatg tcgggcaggc gcttgtcgtc gcccgtgccg atcgcaccgg
      361 acagagcgcg ctggaggatg ccatcgcgct cctctccggc tgcccgaaca tccagttgct
      421 gctgaacgcg gtgcagttct cgccgagcgg gcggcggttt gggtcttact acggatatac
      481 gggatagggc tggggcatgg accggatcag gaccaccgcg atactggcgg gcgcgctgct
      541 cgccgggatc ggcttcccgg caggagcggg ggcgcaatcg atatcctatg gcaaggcctc
      601 gccgggggcg ggcgagggcg ggccctcagc ctcgtcggac gacaatgccg tggaggcccc
      661 ggcctcatcc tcgggtgaaa gacgtggccg ccatggccgc ctgcgccttt cgccctatat
      721 cgaggcggcc caggtgatca gcgccgaact cgccccccaa catgacgtgc tgacgtggtc
      781 cacgctcgcc gtcggcgtcg acggcggggt ggcgggccgc aacacccagg ccagcttctc
      841 cgtgcgttac gagcgccgct ttggctatgg caagtcggac agcggcgata cgatttcggg
      901 cctggcccgt gcctcggtcg gcctggtccc gcgggtcctt tcgttcgacg tcggcgcgct
      961 tgccacccgc accaccaccg acggcggcgc ggcatttccc ggcagcgttg accctaaccg
     1021 ttcgagccgc ctgtggtcgg cctatgccgg ccccagtctc tccacccacg ccggcgacgt
     1081 ggcggtcaat ggcgcctacc gggtcggcta caccgaggtc ggctcgaaca gcgcggtgac
     1141 gaacaacggg gtcggcggag tggtcgacct gttcgaccat tccgtaaccc agatggcttc
     1201 gctccatgcc ggccttgccc cggggcatgg cctgccgatt ggcgtgggcc tgggcggcgg
     1261 ctattaccac gaagacatct ccaacctcga ccagcaggtg aaggacctca gcctgcgcgc
     1321 cgacgtggcc gtgccggtgt ccgacacggt ctcgctggtt ggcggggtgg gctatgagaa
     1381 cgtgcgcgtg tccagccgcg atgccctgcg cgatccgctg accaacgcgc cggtcgttgt
     1441 gggcggccgc tacgtcaccg acaagagcgc cccgcggcag attgcctatg ataccagcgg
     1501 cttcatctgg gatgccggag ttacctggcg ccccagccgg cgcacggcct tcgaggccca
     1561 tgtcggccgg cgctatggtt cgaccaccta ctacggctcg ttcggctggc gcgcctcgcg
     1621 ccgcagcacg gtcaacatct cggtttacga cagcatctcg ggcctgggcg gctcgctcaa
     1681 ccgggcgctg gttgcactgc cgaccgattt cgaggtcagc cgcaacccgg tgaccggcga
     1741 tatcaacggc tgcgtctcgg cgctcggcaa cgggtcgggc tcgagcccct gcctgatcgg
     1801 cgcgctgggc gccgtccgct cggccacgtt ccgctcgcgc gggatccagg gcagctggtc
     1861 ggtccaggcc agccgcttcg gcttcgggtt cggcgcaggc tacgaccggc gcaagttcat
     1921 cgccgccccg ggcacgatcc ttgccgccgc ttcaggccag accgacgaga gcgcgtggct
     1981 ctcggcctgg ctttccggtc ggatcgaccg cgattccagc ttctccacct ttgcctatgt
     2041 cgactggttc cagagcgggc tcgacggcag cgacggcacg gcgatcggcg ccaatgcctc
     2101 ctacagccgc gcgctgggcc accatctttc cgccaacgcc gccctctcta tccagggcgt
     2161 ccagcatagc gacggccccg atacctggga cgcctcggcc ctgctgggca tgcgttattc
     2221 cttcttctga gcgatcggga acacgagcta tgtacgaaga attctatggc ttcaccgggc
     2281 gcccgttcca gctcacgccg gacgccgcgt tctatttcga gagcctgacg caccgcaagg
     2341 cgctgtccta tctcggctat ggcctggccc agggcgaagg cttcatcgtc atcaccggcg
     2401 agatcggctc gggcaagtcg accctggccg cccacctcat ggcgacgatc gaccccgagc
     2461 ggctgaccgc ggcccaagtg gtcaccagcg cgctcaacgg ggacgaaatc atccacgtcg
     2521 tcgcccagtc cttcggcctc gttatcgacg ccaacgacaa ggcgacggcg ctggccggca
     2581 tcgagggcct gctccatgcc gaggcgcgcg gcggccggcg ctgcctgctg gtggtcgacg
     2641 aggcgcagaa cctctcgatc gaggcgctgg aagagctgcg catgctgtcc aacttccagc
     2701 ttggcgcgca gccgctgctc cagaccctct tgctcggcca gccggaattc cgcgacacgc
     2761 tgcaatccag cccacgcctc gaacagctgc gccagcgcgt aatcgctacc catcacctcg
     2821 atgcgatgga tgccgacgag gtccagccct atgtcgagca tcgcctgaga ctggttggct
     2881 ggaacggccg accctctatc gagaccccgc tctatccggc gctctatgag gccaccggcg
     2941 gcgtgccgcg gcggatcaat cagatcgcca accgcctgtt cctgatgggc tgcgtggagg
     3001 aacgcgatca cttcgacatg accctgttcg agcgggttat ggaagaggtc gcgacagata
     3061 ccgcgctcgc cccggctgcg cccgtggctg aggctccggt tcacccggcc gagcaggcgc
     3121 ccgccgttgc agccgcgccc atcgatgcgg tgcccttcgc ggagatgaag gaagccttcg
     3181 acgagcgcga cgtcctcatt tccgagctgc agcaggccgt gatcgagctg gccaacaccc
     3241 tcgaaggcca gcccgcgccg gttctcgccg acagcgggat caaggccgat ctggccgcgc
     3301 tcaaccagcg tctgagcggc atggccgacc ggctcggttc gctcgaggcg cgcatggtcg
     3361 agcaggaagc cagcgtcaag cacacgcttg ccatgctgat cgaatggctg gagagcgatc
     3421 agaaggccgc ggcctgaacg ggccgggaag gggcccttca tgcgcgtggt caatgccctc
     3481 tcggtcgatg tcgaggaatg gttccaggtc ggtgccttcg agcgggtgat cgcccgcgac
     3541 gaatgggacg gccttgcctc gcgggttgag gacaactgcg cgcggattct cgacctgttc
     3601 gccggtgcgg gggtttcggc cacgttcttc accctcggtt ggatcgccga gcgccatccc
     3661 gcgctggtgc ggcggatcca tgaggcgggc cacgagatcg ccagccacgg ctgggaccac
     3721 gcccgggtct tcacccttgg cgcgaaagcc ttcgccgcgg atctcgagcg cagccgcaag
     3781 gcgctggaag atgccgccgg agcgcccgtg cgcggctacc gtgcgcccag cttttccatc
     3841 gatgcgcgta cgccctgggc cttcgcggtg ctggcggagc agggctatgc ctattcttcc
     3901 agcgtcgccc cggtggttca cgatcactac gggtggcgcg aagccccgcg cttcgccttc
     3961 cgcccgctgg ccgacagcgc gctggtcgaa ctgccggtta ccaccgccga attcggcgga
     4021 cgccgcctgg ccgcaggtgg cggcgggttc ttccgcgtcc tgccctatgg cttcacccgc
     4081 gccgcgatcc ggcaggtcaa cgggctcggc cagccggccg tgttctattt ccatccgtgg
     4141 gagatcgacc cggcccagcc gcgcgtcggc catgccccgc tgcgctcgcg cctgcggcac
     4201 tatactggcc tcgcgcggat ggcaggcaag ctggagcggc tgaccggcga attcgcctgg
     4261 ggccgggtcg acgccgccgt tgctggcctg agctccgaca ccgccccgtc gccgctggcg
     4321 ctcgccgcat gaatgcgcct ttcgccttgc ccaagctgac cgtccggctc gccgatgcgg
     4381 ccgagccggc gattgccgct ttcctcgccg atcacccgca ggccaccgtg tttcatggcg
     4441 cggcttggct ggaagcgatc tccgcggcca cgggaagcga tgcgctcgcc ctggttgccg
     4501 aagcgcgggg cgaggtgagg ggctacctcc cgctgagcga aatccattcc cccgtgttcg
     4561 gccggttgct agcctcgacg ggctttgccg tgggaggcgg cctgctgctg gccgaaggca
     4621 ccgatgccgc gcccctgctg gcggcggcaa gcgaactggc ccagcgccgc tcctgcccgg
     4681 ccatcgaact gcgcggcggg gtcatgccgg cagacggcac ggggtggcat ctaaagcggg
     4741 aggcccactg cggcttcgtc gccccgcttg gcgagagcga tgaggcgcaa ctgctcgcca
     4801 tcccgcgcaa acagcgcgcg gaagtgcgca aggggctgga gcaggacctt gccgtcacca
     4861 tcgggactgg ccagcgtgac cgggccgccc atcatgcggt ctattcggaa agcgtgcgca
     4921 acctcggcac tcctgtcttt ccccgcgcgc tgttcgactg cgtgctcgac cggctgggtg
     4981 aaaacgccga tatcctcacc gtctggcggg gcgagacgcc ggttgccagc gtactctcgc
     5041 tctaccaccg cagtgcggtc atgccctatt ggggcggagg cacccgcgcc gcgcgcgccc
     5101 tgcgcgcgaa cgaccggatg tatttcgaac tgatggccca cgcccgccgg cgtggctgca
     5161 cccgcttcga tttcggccgc tccaagaccg gcagcggccc ttaccatttc aagcgcaact
     5221 ggggcttcgt gcccgagccg ctgacctatg ccaactggac cgcgccgggt tgccaaccgc
     5281 gcgatgccga tccgaccagc gccaagcacc gggcgctgat cgcggcctgg caattgctcc
     5341 ctctcgccgt cgccaaccgc ctcggcccgc tgatcgcgcg ggggctgggc tgagccgtgg
     5401 gcgaaatcct gttcatcgcc catcggctgc cgttcccgcc ggaccggggt gacaagatcc
     5461 gctcgcacta cctgctgcgg catctggccg ggctggggcc cgtccacgta gcctgctttg
     5521 ccgacgatga agccgacgcc gtgcacgagg gcgaactcgc cgcccttgcg cagtcccact
     5581 gcctcgtccc gcgtagccgg cccctgccgc tggcgggcct cgcggcgctg gcgaccggcc
     5641 ggccggtcag cctcgccgct tttgccgatg cgcggctgca ggcctatgtc gatcgggtgg
     5701 ccgccacccg cccgttggcg gccatcgtgg tgttttccgg tcagatgggg caatatgtcc
     5761 cggctggctg gaccgggccg ctcgtcgtcg atttcgtcga cgtggattcg gccaagttcg
     5821 gagcttatgc ggcggcggcc cgcggaccga tgagctgggt ccacgcccgc gaggaccgcc
     5881 tgctccgggc cgaggaaggc cgacttgccg cccgcgccga tgccagcctg ctggtgagcg
     5941 agccggaagc cgagctgttc cgcacgcgcc ttggcccgga ccggcaaggc cgctgcaagg
     6001 tagtcgccat cggcaacggc atcgatgtcg cggcctatga tcccgaggcg gtcgcgcccg
     6061 agccgcgcat ggccgccatg cccggccccc gtgtgatctt taccgggcag atggactacc
     6121 cacccaatgt cgccgccgtg gtccgcgcgg ccagccgcat catgccgctg gtgcgccggt
     6181 tcttgcccgc agcgaccttc cacgtggtcg gccgcaagcc agcggccgag gtcatggcgc
     6241 tgcatggcag taacggctgt cacgtctggg gcagggtcga cgatatccgg ccctggctgc
     6301 gcgccgcgga cattgctctc gtgccgctcg agattgcgcg cggggtgcag aacaaggtgc
     6361 t
//
LOCUS       DATNBV010000125         2508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3267791,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000125 DATNBV010000000
VERSION     DATNBV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3267791"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..663
                     /locus_tag="VN627_03030"
     CDS             466..663
                     /locus_tag="VN627_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02261.1"
                     /translation="MTAIDRIDALLAGITKADIQAMPSALRRRLAQALRRIADLADPL
                     AKPAEADVGVLADLKDGQQIE"
     gene            774..1022
                     /locus_tag="VN627_03035"
     CDS             774..1022
                     /locus_tag="VN627_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02262.1"
                     /translation="MTATLAERPQRWSWSPDRRRAYSRFMTEVWAQRERKPIAADPER
                     GLGYLDRGSARTFEESLARARDPRRRVRQETNWSKCRP"
     gene            1019..1189
                     /locus_tag="VN627_03040"
     CDS             1019..1189
                     /locus_tag="VN627_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02263.1"
                     /translation="MSNVVQIKATESAGLPANLNIDRAKLPDANVAAKAALAKCYAVD
                     ERPHRHGIEPLP"
     gene            complement(1555..>2508)
                     /locus_tag="VN627_03045"
     CDS             complement(1555..>2508)
                     /locus_tag="VN627_03045"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXD02264.1"
                     /translation="SPCLVGIEACATSHHWSRELQALRHTVRLMPPAYVKSYLKRQKN
                     DAADAEAICEAVARPNMRFVETKTAEQQSCLMLHRTRHLFVRQQNAVINSIRAHLAEL
                     GIVAPVGRNGVEELLDVVADPEDDRVPEVARECLAALGAQLRMLKAQILEFDQRIVAW
                     HRSNETSKRLDEIPGVGPALATVLVASVADPRAFRSGRDFSAWIGLVPKQNSSGGKEK
                     LGNITKRGDRYLRSLFTAGAGGDPLCEAPWHQAPAMAHRTVGAAANQGGGHCARQQDR
                     PHGMGHDGQRRALQGTCRPGGMNEIAPVSWRDAEVGRTNST"
BASE COUNT          444 a          820 c          788 g          456 t
ORIGIN      
        1 gcaccgccgc gccgggcgcc gagcgtgatc ccaggaatga agccgaccgc tttccccgtc
       61 agcgtcttcg ccgttgatag cgccggtgcg gtgctggagg acgtcttggt cctgtcggcc
      121 gagagccacc actaacgaag atcgggtcgc cccgcgcgtg gcgcgaccct tctgtaggca
      181 ttttccgagt ctcgccggtc atgcagcggc gtgcgcttgt gcgggctagg gggaacgcgt
      241 tgattggcct ctctccgcgg aacctccgcc aatggtccgg cgtgagcaaa ccccgctaac
      301 cggatcattg gctctcccgt taccccgcgc actcgggcca acccgggaaa gtcgttgcca
      361 ccttggaacg attttcggtc tgccgacctt ggcaacctca agggcgagat gtccgtgctc
      421 gtgaaggcaa gcgcgtagac gagagcgtat gggagttacg ttatggtgac cgcgatcgac
      481 cggatagatg cgctgctcgc cggcatcacg aaagccgata tccaggccat gccgtctgcg
      541 ctccgccgcc ggcttgcaca ggcgcttcgc cggatcgccg acctggctga cccgctggcc
      601 aagcccgcgg aggctgacgt aggcgtactc gccgacctca aggacggcca gcagatcgaa
      661 tgagatcgag ctcgaccggg cccggccagc gccactcttt catcacgcca cgcaccgaac
      721 cggcgtcggc gcgcgagttg gggcaacgag gtcacgaaat tcggagaggc agcatgaccg
      781 caaccctggc cgaacggccc cagcgctggt cctggtcccc cgacaggcgg cgcgcctaca
      841 gccgcttcat gaccgaggtc tgggcgcaac gggagcgcaa gcccatcgct gccgatcccg
      901 aaaggggcct gggatacctc gaccggggca gcgcccgcac cttcgaggag tccctggcga
      961 gggcgcgcga tccacggcgc agggtgaggc aggagacgaa ctggagcaag tgcaggccat
     1021 gagcaacgtc gtccagatca aggcgaccga atccgctggt ttgcctgcga acctcaatat
     1081 cgaccgagcg aagctgccgg acgccaacgt cgcggccaag gccgcgctcg cgaagtgtta
     1141 cgcggtcgat gaacgtcccc accgacatgg gatcgagccc ttgccataaa ccgtgttagc
     1201 gaactccctc ccccaattcg cagcttggcg cgatggcacc gtgcccggca ctgtcagcgt
     1261 atccaggctc ttggcagatt ttccgtcgcc tgtacggtag gtttcagagc gaacctgccg
     1321 caagatcaat gggctatggc agggtagtgc catgtgtgga cggctccgtg ttggcaagga
     1381 ctttcttcac gtcgcagttc tggtcggaac agccatgtgt tcggcctatt gatgcggctc
     1441 gcatgaccgc tggccataat gccttccgcg gatcgggtcc cggtcaaaag cccgcactcg
     1501 acagtgctgt ggcactagtg ggttgtcccg atctccggat cgaccggatc tgcattacgt
     1561 gctgttcgtc cttccaacct ccgcatcacg ccagctgacc ggcgcgatct cgttcatgcc
     1621 gccagggcgg caggttcctt gtagcgctcg cctttggcca tcatggccca tgccatgcgg
     1681 gcgatcttgt tggcgagcgc aatggccgcc accttggttg gccgccgcgc caacggtgcg
     1741 gtgagccatg gccggtgctt ggtgccatgg agcttcgcat agcggatcac cgccagcgcc
     1801 tgctgtaaac aggctgcgca gatatcgatc gcctcgcttg gtaatgttgc cgagcttctc
     1861 cttgccaccg ctcgagttct gcttgggaac cagcccgatc caggccgaaa agtcccgtcc
     1921 cgatcggaaa gcccttggat cggcaacgct ggccacgagt acggtggcaa gtgccggccc
     1981 gacgccaggt atctcatcca gccgtttgct tgtctcattg gagcgatgcc aagccacgat
     2041 gcgctggtcg aattcgagga tttgcgcctt cagcatccgc agctgtgcac cgagagcggc
     2101 cagacactcg cgcgcgacct ccggcacgcg atcatcctca ggatcagcca caacatcgag
     2161 cagctcctca acgccgttgc gcccgaccgg cgccacgatg ccaagctcgg caagatgcgc
     2221 ccggatggaa ttgatgacgg cattctgctg gcgcacaaat agatggcgcg tgcgatgaag
     2281 catcaggcag ctttgctgtt ctgccgtctt ggtttcgacg aaccgcatgt ttggccgagc
     2341 aacggcctcg cagatcgcct cggcatcggc ggcatcgttc ttctggcgct tcaggtagga
     2401 cttcacgtag gccggcggca tcagccgaac ggtgtggcgg agcgcctgca actcccgtga
     2461 ccaatggtgc gatgtggcgc aggcctcgat gcccaccagg cagggcga
//
LOCUS       DATNBV010000126         4008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3267811,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000126 DATNBV010000000
VERSION     DATNBV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3267811"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..692
                     /locus_tag="VN627_03050"
     CDS             234..692
                     /locus_tag="VN627_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXD02265.1"
                     /translation="MRKIILPLVASLALATPALANEARVEARGGVAWLPGTSKGTAGI
                     AAGYDYDLGSAAFVGAEVSADKVLTSGTKVAFGFTGRAGAKLNKVKLFAAGGYTTEIA
                     SGFEGNWHAGAGANLDITNNVYLTTEYRHFFKNGNGFDADAVVAGVGLKF"
     gene            complement(804..1442)
                     /locus_tag="VN627_03055"
     CDS             complement(804..1442)
                     /locus_tag="VN627_03055"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HXD02266.1"
                     /translation="MPAPAIKICGVTTPEALDAVIAARAEYVGFVFFPKSPRNLAPGA
                     AAALAARAEGRVARVGLFVDADDAAIGEAVSAARLDGLQLHGGESPERAAQLRARFGL
                     PVWKAVSVASAADVDRAAAYVGAVDLVLFDAKTPKGTLPGGMGLAFDWSLLAGWKNRA
                     AWGLAGGLNPGNVAEAVRMTGAPLVDTSSGVESAPGVKDTGLIADFCRAARG"
     gene            complement(1517..2983)
                     /locus_tag="VN627_03060"
     CDS             complement(1517..2983)
                     /locus_tag="VN627_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXD02267.1"
                     /translation="MQIDFEALFAASPNPYVVLDPALVIVWMNDAYLAATMRERADIL
                     GRPMFEAFPSSPSSDSHRLLAASFQQVLRTRQVDEIALIRYDIANPDGEMDVRYWSAT
                     HTPLLDAQGNIALILQHTVDVTELQDLRAMRDEMGLVQRAQAVQARNQDLVAEGGRMR
                     ALFEQAPGFVAVVSGPDHRFQMANAAYRTMVGHRDLIGKTVAEALPEVIEQGFVDLLT
                     EVRSSATAYVGHREKVILKNEPDEAGGERYLDFLYQPIVAEDGEVSGVFIQGHDVTAH
                     VQAEEHQLLLINELNHRVKNTLAIVQGLATQSFRRAESVEAASRTFTARLMTLARAHS
                     QLTQNKRETAGLAETIRAEVEATAGSDAERVRTHGPAASLEPQAAMSLAMIVHELSTN
                     AIKYGALSTERGGIDVTWSSTDVGDVREIALDWVESGGPPVVAPLRRGFGTRLIERGI
                     ASGLKSDVALEFKPEGLHCRVVVRQSGEHQWDYAISRD"
     gene            complement(3273..>4008)
                     /locus_tag="VN627_03065"
     CDS             complement(3273..>4008)
                     /locus_tag="VN627_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014809.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HXD02268.1"
                     /translation="AAALAAAAAPQFAAAQTGPAPVLAVGGTLLTVTADGRVTRAPDI
                     ATFSAGVVSQGKTASEALAANAADMSRVIAALKRAGIADKDIQTSNLSLNPVYQPQRN
                     MPDGTIEPPQPRIVGYQANNSVNVRQRNLKDYGKVIDTLVTAGANQVNGPSFEVENPD
                     PVLDAARAAAMQKARARAELYAHAAGLRVSRILSISESGGWSPPQPVMYRMAAMEAAP
                     PAPPSPVQAGELQMSMNVTVQFELVP"
BASE COUNT          643 a         1410 c         1263 g          692 t
ORIGIN      
        1 gaatgtccag attcgccctt cgggcaggtc gccccagacc cgtcgcctgc cctcggaaca
       61 gccgttcgtt tcaatgcaaa cttgcgttgc tgcagggcaa cacacgcagt gcaattgcgc
      121 taaactattc tattcgttca ttgcggcgac agattccacc ccctaccccg cactccgccc
      181 ccggcagaag gggcaaccgg cactgtccgg actttgaact caggagtaat accatgcgca
      241 agattatcct ccccctcgtc gcttcgctcg ccctggcgac cccggccctc gccaacgaag
      301 cccgcgtcga agcgcgcggc ggcgttgcct ggctgccggg cacgtcgaag ggcaccgccg
      361 gcatcgccgc gggctacgac tacgacctcg gcagcgccgc gttcgtcggc gccgaagttt
      421 cggccgacaa ggtcctgacc tcgggcacca aggtggcctt cggcttcacc ggccgcgccg
      481 gtgcgaagct caacaaggtc aagctgttcg cggctggtgg ctacaccacc gaaatcgctt
      541 cgggcttcga gggtaactgg cacgccggtg ccggtgccaa cctcgacatc accaacaacg
      601 tgtacctgac caccgagtac cgccatttct tcaagaatgg caacggcttc gacgccgacg
      661 ctgtggtggc cggcgttggc ctgaagttct gatcgcacca gcgacagacc tgaaacagag
      721 aagggcggcc ttaacgggcc gccctttttc tgttgttcct cccgagcgag gccgaggggt
      781 cataccgagc gtcagtttac ggactaaccc cgcgccgccc ggcagaaatc cgcaatcaac
      841 cccgtgtcct tcacccccgg cgcgctctcc acgccggaag aggtatccac cagcggcgca
      901 cccgtcatcc gcaccgcctc ggcgacgttc cccggattga gcccgccggc cagcccccag
      961 gcggcccggt tcttccaccc ggccagcaag gaccagtcaa acgccagccc catgccgccc
     1021 ggcaatgtgc ccttgggcgt cttggcatcg aacagcacca ggtcgaccgc gcccacataa
     1081 gccgccgcgc gatccacgtc tgccgcgctg gccacggaaa cggccttcca caccggcagc
     1141 ccaaaccgcg cgcgcagctg cgccgcccgc tccgggctct cgcccccatg cagctgcaac
     1201 ccatcgagcc gcgccgcgct caccgcctcg ccgattgccg catcgtcggc atcgacaaac
     1261 agccccaccc gcgcaacccg gccctctgcc cgcgccgcca gggcagcggc cgcgcccggc
     1321 gcgaggttgc gggggctctt gggaaaaaac acgaatccca catactccgc ccgcgcggca
     1381 atcaccgcgt cgagcgcttc gggcgtggtc acgccgcaaa tcttgatggc cggggcgggc
     1441 atggggcgca tttagcccac tggcacggat ttgctagggg caggggacta accacccgac
     1501 ggttgccctc gggcgatcag tcacgagaaa tcgcgtaatc ccactggtgc tcacccgatt
     1561 gccgaacgac gacccggcag tgcagcccct ccggtttgaa ttccagggca acgtccgatt
     1621 ttaggcccga cgctatcccg cgctcgatca ggcgcgtacc gaagccgcgg cgcagcggcg
     1681 caacgaccgg cgggccgccg ctttcgaccc agtccaaggc gatctcccgg acatcgccga
     1741 catccgtcga cgaccaggtc acgtcgatgc cgccccgctc cgtggacagg gctccatatt
     1801 tgatcgcatt ggtgctgagc tcgtggacta tcatcgccag tgacatcgcg gcctgcggtt
     1861 caaggctggc ggcgggcccg tgggtgcgga cccgctctgc gtcggacccc gccgttgctt
     1921 cgacctccgc ccggatagtc tcggcgaggc ccgcggtctc ccgcttgttc tgcgtcagct
     1981 ggctgtgcgc cctcgccagc gtcatcagcc gggccgtgaa tgtccgactg gcggcctcca
     2041 cactttccgc gcgccggaaa gactgcgtgg ccaagccctg gacgatggcc agcgtgtttt
     2101 tgacccggtg attgagttcg ttgatcagga gcaactggtg ctcctcggcc tgcacatgcg
     2161 cggttacatc gtgtccctgg atgaaaacgc cggaaacctc tccgtcttcc gcaacgatgg
     2221 gctgatagag gaaatccagg tatcgctcgc cgcccgcttc gtcaggctcg ttcttgagga
     2281 tcaccttctc gcggtgcccg acataggctg tcgcgctgct gcggacctcg gtcagcagat
     2341 cgacgaagcc ctgttcgatc acttctggca gagcttccgc aacggtcttg ccgatcaggt
     2401 cgcgatggcc aaccatggtg cggtaggccg cattggccat ctggaaccgg tgatcgggcc
     2461 cgctgaccac ggcgacgaag ccgggcgcct gctcgaacag ggcgcgcatt cgcccgcctt
     2521 ccgcgaccag atcctggttg cgggcctgca ctgcctgggc tcgctgcacc aggcccattt
     2581 cgtcgcgcat tgcgcgcagg tcctggagtt cggtgacgtc aaccgtgtgc tggaggatca
     2641 aggcgatgtt gccttgggca tcgagcagcg gtgtgtgcgt agcgctccag taccgcacgt
     2701 ccatttcccc gtccggattg gcgatgtcgt agcggatcag cgcgatttca tcgacctgtc
     2761 tggtcctcag aacctgctgg aacgaggctg ccagcaggcg atggctgtcc gacgaaggac
     2821 tgctgggaaa cgcctcgaac atcggccggc ccaggatatc ggcccgctcg cgcatggtcg
     2881 cggcgagata ggcgtcgttc atccagacga tgacgagggc cggatcgagc accacgtagg
     2941 ggtttggcga tgccgcgaac agtgcttcga aatcaatttg caccagctag cccctggata
     3001 ggaatgtcgc cgcccaactt cctgcagtcc cgttggcgct ggcgagccgg acggtgcgaa
     3061 tcacatcgac cagtgccgtc gcagcctccg gccattggca cgtaactgtc tgattagcca
     3121 aatcttccac ctcgctccag caaaccacgg ggcgaccgga acggctgcat tcgctggggg
     3181 tggcggactt gaagcgcggc tccgcccgtc gccccgactt gtttcggggc cgggctgttc
     3241 cataaacctg cgagcctgcc gcgggtggcg ccttacggca ccagctcgaa ttgcaccgtc
     3301 acgttcatgc tcatctgcag ttcgcccgcc tgcaccggcg agggcggggc cggcggggcg
     3361 gcttccatcg ccgccatgcg gtacatcacc ggctgtgggg gcgaccagcc gccgctttcg
     3421 ctgatcgaga ggatgcggct cacgcgcagc cccgccgcgt gggcatagag ctcggcgcgg
     3481 gcgcgggcct tctgcatggc ggcggcgcgc gcggcgtcca gcaccgggtc ggggttctcc
     3541 acctcgaagc tcggcccgtt gacctggttg gcgcccgcgg tgaccagcgt gtcgatcacc
     3601 ttgccataat ccttgaggtt gcgctggcgc acgttgaccg aattgttggc ctggtagccg
     3661 acgatccggg gctgcggcgg ctcgatcgtc ccgtcaggca tgttgcgctg gggctggtag
     3721 accggattga gcgagaggtt gctggtctgg atatccttgt cggcgatccc cgcccgcttg
     3781 agcgccgcga tcacccggct catgtcggcg gcattcgcgg ccagtgcctc gctggcagtc
     3841 ttgccctggc tgaccacgcc ggcactgaag gtcgcgatgt cgggcgcgcg ggtcacgcgg
     3901 ccgtcggcag tcaccgtcag cagggtgccg cccacggcca gaaccggagc gggcccggtc
     3961 tgcgccgcgg caaactgcgg ggcggctgcg gcggcgagcg ctgcggcg
//
LOCUS       DATNBV010000127         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2641760,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000127 DATNBV010000000
VERSION     DATNBV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2641760"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VN627_03070"
     CDS             complement(<1..299)
                     /locus_tag="VN627_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02269.1"
                     /translation="MKSGLRAFGAIVWAAPVVAQPASDPLAPLPQGTQATAPVPPPAP
                     VAIQPAPAPAIPALPAVPTVVVPRDWRGVFDAIDAGNWAAARAGIAALPPGILTPV"
     gene            complement(296..991)
                     /locus_tag="VN627_03075"
     CDS             complement(296..991)
                     /locus_tag="VN627_03075"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HXD02270.1"
                     /translation="MDSIGIAEARGALAWWLEAGVDVAVQDSPRDWLKPTSRAPTIAA
                     PVANVATPTHSTLAELQHWLASSAELPLASATARRILPHGPENAPIMLLSDAPALEDF
                     AAGQPIGGEAWALAGRMLAAIGIDSAESYSASLSCFNTPGARLADPEREACAEIARRH
                     IRLVQPERLILFGDGPALALLGKRLVEARGHIHKVEGVRAIATFHPRHLIKRPLDKSL
                     AWRDLLLLMEDKS"
     gene            1108..>2729
                     /locus_tag="VN627_03080"
     CDS             1108..>2729
                     /locus_tag="VN627_03080"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HXD02271.1"
                     /translation="MSERESMPYDVVIVGGGPAGMTAAIRLKQLANAAGRDVSVCILE
                     KGSEVGAHILSGAVLDPKALDELIPDWNDKGAPLTVPVSENHHWILGKSGKFAMPHFL
                     LPPFMHNKGTYTLSLGNFCRWLAGQAEELGVEIFPGFPAAEILFNDDGSVKGVATGDM
                     GIARDGSHRPDYQPGLELHAKYTLFAEGARGHLTKRLTEIFKLRDESGPQVFGLGVKE
                     LWDVPPEKHKPGRVIHTQGWPLRDAWGGGFLYHQENNQVALGFIVSLDYSNPYISPFD
                     ELQRWKTHPAIRTEIEGGRRVSYGARAINEGGWQAIPRLVFPGGALIGCSAGFVNVPR
                     IKGTHNAMKSAMLAAEAAFEAISQDRSGDLLEAYTAALHKSWVVKELKIVRNAQPAVA
                     HWGGMLGTLYAGLDMWLGYLGLRLPWTLKHKADNTTLREKAAARPIDYPKPDGVLTFD
                     RLSSVFISNTNHEEDQPVHLTLKDPDIPTTINLPVYAGPEQRYCPAGVYEFVEDGNGE
                     PRLQINAQNCVHCKTCDIKDPTQNINWVTPEGG"
BASE COUNT          474 a          893 c          953 g          409 t
ORIGIN      
        1 acgggggtga ggatgccggg cggaagggcc gcgatcccgg cgcgcgccgc cgcccaattg
       61 ccggcgtcga tcgcgtcgaa cacgccgcgc cagtctcttg gcacgaccac ggtcgggact
      121 gcgggcaagg ccggaatcgc cggcgcgggg gcgggctgga tggctaccgg tgccggcggc
      181 ggtaccggcg cggttgcctg agttccctgc gggagcggag cgagcggatc ggacgcgggc
      241 tgcgcgacga ccggcgcggc ccagacgatc gccccgaacg cgcgcaggcc tgacttcaag
      301 atttgtcctc catcagcagc agcagatccc gccatgccaa cgacttgtct aacggccgct
      361 taatcaggtg gcgggggtgg aaggtggcga tcgcacgcac gccctcgacc ttgtggatgt
      421 gcccgcgcgc ctcgaccagc cgcttgccga ggagggcgag cgccggcccg tcgccgaaca
      481 ggatcagccg ctcgggttgg accaggcgaa tgtggcggcg ggcgatctcg gcgcatgcct
      541 cgcgctccgg gtcggcgaga cgggcgccgg gcgtgttgaa gcaggacaaa gacgcgctgt
      601 aggactctgc ggagtcgatc ccgatcgcgg cgagcatgcg cccggcgaga gcccaggcct
      661 cgccgccgat cggctggccg gcggcgaaat cctccagcgc cggagcatcg ctgagcagca
      721 tgatcggcgc attctccggc ccgtgcggga gaattcgccg ggcggtcgcg gaggccagcg
      781 gcagctcggc gctgctcgcc agccaatgct gcagctcggc gagcgtgctg tgggtgggcg
      841 tggcgacgtt cgcgaccggg gcggcgatgg tcggcgcgcg agaggtgggc ttcaaccagt
      901 cgcgcgggct gtcctgcacg gcgacgtcga cgccggcctc gagccaccag gccaatgcgc
      961 cgcgcgcctc ggcaatcccg atcgaatcca cgtccccgcc cagagtcatc tcccttggct
     1021 cttcctcgcc cggcattgac ggaagcgtca aggaatgggc agggcagacg agtcgaattg
     1081 tgtgggtaac gcggccaagg gaggatcatg agcgagcgcg aatccatgcc ctatgacgtg
     1141 gtgattgtag gcggcggccc cgccggcatg accgcggcga tccgcctcaa gcagctggcg
     1201 aatgcggccg gacgcgacgt ttcggtctgc atcctggaga aaggcagtga ggtcggggcg
     1261 cacatcctgt ccggcgccgt gctcgacccg aaggcgctcg acgagctgat cccggactgg
     1321 aacgacaagg gcgcgccgct gaccgtgccg gtcagcgaga atcaccactg gatcctcggc
     1381 aagagcggca agttcgcgat gccgcacttc ctcttgcccc cgttcatgca caacaagggg
     1441 acgtacacgc tgagcctcgg caatttctgc cgctggctgg cggggcaggc ggaggagctg
     1501 ggagtcgaga ttttccccgg cttccccgcc gccgagatcc tcttcaacga cgacggctcc
     1561 gtgaaggggg tggcgaccgg cgacatgggg atcgcgcgcg acggctcgca tcggcccgac
     1621 taccagccgg gcctcgagct gcacgccaaa tacaccctgt tcgccgaagg cgcgcggggc
     1681 catctgacca agcggctgac cgagatcttc aagcttcgtg acgagagtgg gccacaggtt
     1741 ttcggtctcg gtgtcaagga gttgtgggac gttcctccgg agaaacataa gcccgggcga
     1801 gtgatccaca cccagggctg gccgctccgc gacgcgtggg gcggcgggtt cctctaccac
     1861 caggagaaca accaggtcgc gctcggcttc atcgtctcgc tcgactattc caacccctac
     1921 atttcgccgt tcgacgagct gcagcggtgg aagacccatc cggcgatccg gacggagatc
     1981 gaaggcgggc ggcgcgtgtc ctacggggcc cgggcgatca acgagggcgg gtggcaggcg
     2041 attccccggc ttgtgttccc cggcggcgcc ctgatcggct gctcggcggg cttcgtgaac
     2101 gtcccgcgga tcaagggcac gcacaatgcg atgaagtcgg cgatgctcgc cgccgaagcc
     2161 gcattcgagg cgatatcgca ggatcggtcc ggcgacctgc tcgaggccta taccgcagcc
     2221 ctgcacaaga gctgggtcgt caaggaattg aaaatcgtcc ggaacgcgca gcccgccgtc
     2281 gcccattggg gcgggatgct cggcaccctc tacgcaggat tggacatgtg gctcgggtac
     2341 ctcggcctgc gcctgccgtg gacgctcaag cacaaggccg acaacacgac cttgcgcgag
     2401 aaagccgcgg ccaggccgat cgactatccc aagccggacg gggtgctgac cttcgaccgt
     2461 ctgtcgtcgg tgttcatctc caacaccaat cacgaggagg accagccggt tcatctgacg
     2521 ctcaaggacc cggacatacc gacgacaatc aacctgccgg tctatgccgg gcccgagcag
     2581 cgttactgcc cggcgggcgt ttatgaattt gtcgaggatg gaaatggcga gccgcggctg
     2641 cagatcaacg cccagaattg cgtccactgc aagacgtgcg acatcaagga cccgacccag
     2701 aacatcaact gggtcactcc cgaaggcgg
//
LOCUS       DATNBV010000128         2617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1668726,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000128 DATNBV010000000
VERSION     DATNBV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668726"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VN627_03085"
     CDS             <1..458
                     /locus_tag="VN627_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HXD02272.1"
                     /translation="DIGMIDIAGSFGAKVYYGDGTRMDVLRQAGAADAEMIMFCIDGD
                     QLTADFLEGVHKAFPQAALYVRAFDRRAVVALGQAPVRKLVREVLESAVVMARRALSD
                     SGLALEQIDRAEAMYRARDRERLRLQVETGDIRVARDRIITERIGEGEG"
     gene            complement(756..2135)
                     /gene="rimO"
                     /locus_tag="VN627_03090"
     CDS             complement(756..2135)
                     /gene="rimO"
                     /locus_tag="VN627_03090"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HXD02273.1"
                     /translation="MAVEIPSPRKVGMVSLGCPKALVDSERILTRLRADGYAMSPDFA
                     GADVVLVNTCGFLDSAKEESLAAIGEAIAENGRVIVTGCMGNEAEVIRAKFPDVLAVT
                     GAHQYEAVVEAVHEAAPASLGPYVDLIPQADPGEVKLTPRHYSYLKISEGCNHACSFC
                     IIPQLRGKLASRRIDAVLREAEKLVAAGTRELLVISQDTSAYGVDVRHEERMWKDHPV
                     RTHMTDLARELGQLRTPDGSVPWVRLHYVYPYPHVDAVIPLMAEGLLTPYLDIPFQHA
                     APAVLKAMKRPANEAKVLERIRSWREICPDLAIRSSFVVGFPGETEADFQYLLDWLDE
                     ARLDRVGAFRFEPVAGASANDLPGAVPEAVKEERYARIMEKTEAISAARLAAKVGRVL
                     PVIIDEVGEPDEDGDIGATGRSQADAPEIDGAVFLRNVPEGIAPGSFVNVLVEEADAH
                     DLFGVIAGS"
     gene            2230..2595
                     /locus_tag="VN627_03095"
     CDS             2230..2595
                     /locus_tag="VN627_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HXD02274.1"
                     /translation="MGPVNWLAVALSTLVMLALWAVWPGARRARVTAPGMLGALVLLA
                     LPAAMIGHALARIGPEKLALKPWLYWMQTGGVAAFFIIPALWLARTRSAWNGREALAE
                     AGFWLVAFLAMGTVFWATA"
BASE COUNT          406 a          951 c          826 g          434 t
ORIGIN      
        1 ccgatatcgg catgatcgac atcgccggct cgttcggggc caaggtctac tatggcgacg
       61 gcacccgcat ggacgtcctg cgccaggccg gcgcggccga tgccgagatg atcatgttct
      121 gcatcgacgg cgaccagctc accgccgatt ttctagaagg cgtccacaag gccttccccc
      181 aggcggcgct ctacgtccgc gccttcgatc gccgagctgt cgtcgcgctc ggccaggccc
      241 ccgtccgcaa gctggtgcgc gaggtgctcg aatcggccgt ggtcatggcg cgccgcgcct
      301 tgtccgacag tggcctcgcc ctcgaacaga tcgaccgcgc cgaagccatg taccgcgccc
      361 gcgaccgcga acgcctgcgc ttgcaggtgg aaaccggcga cattcgcgtc gcgcgcgacc
      421 ggatcatcac cgagcggatc ggggaagggg aagggtaaca cccgtcgtcc cgggctcgac
      481 ccgggaccgc tggccaatgc gcagctgtga agctgcttca cgcgcccatg cagctgcccg
      541 gcccgcaccg gtcctggatc aagtccggga cgatgcctgt ccgcaccgcg ccgactctgc
      601 ctgctgcagc gcaaaaaaag ggcccggtcg ttgccgaccg ggccttggaa gttttgggag
      661 aggatgcctg aaaggcaccc cctatgtgcc cccgactcgg ttttgctgca agtgcgaaaa
      721 gagctgagtt agttgcagga aatgcaactg cccgttcagc tcccggcaat caccccgaac
      781 aggtcgtgcg cgtcggcctc ttccaccagc acgttcacga aactgcccgg cgcgatgcct
      841 tccggcacgt tgcgcaggaa aaccgccccg tcgatctctg gcgcatcggc ctggctgcgg
      901 cccgtggcgc caatgtcgcc gtcctcgtcc ggctcgccca cctcgtcgat gatcaccggc
      961 aggacccggc ccaccttggc cgccagccgc gccgcgctga tcgcctcggt tttctccatg
     1021 atccgtgcat agcgctcttc cttgaccgcc tccggcacgg cgccgggcag atcgttggcc
     1081 gaagcccccg ccaccggctc gaaccggaag gcgccgaccc ggtcgagccg ggcctcgtcc
     1141 agccagtcga gcaggtactg gaaatcggct tccgtctcgc ccgggaaacc caccacgaac
     1201 gaggagcgga tcgccaggtc cgggcagatt tcccgccacg aacggattcg ctccagcacc
     1261 tttgcctcgt tcgccggccg cttcatcgcc ttcagcaccg caggcgcggc gtgctggaac
     1321 gggatatcga ggtaaggcgt aagcagcccc tcggccatca gcgggatcac cgcgtcgaca
     1381 tgcggatagg gatagacgta gtgcagccgc acccacggca ctgagccatc cggcgtgcgc
     1441 agctggccca gctcgcgcgc caggtcggtc atgtgggtac ggaccggatg gtccttccac
     1501 atgcgttcct catggcgcac gtcgacgcca taggccgagg tgtcctggct gatcaccagc
     1561 agctcgcgcg tgcccgccgc caccagcttc tccgcctcgc gcagcacggc atcgatccgc
     1621 cggctggcga gcttcccgcg cagctgcggg atgatgcaga agctgcaagc gtggttgcag
     1681 ccctcggaaa tcttcaggta gctgtagtgg cgcggggtga gcttcacttc gccgggatca
     1741 gcctgcggga tcaggtccac atagggccca aggctcgccg gcgccgcctc gtgcaccgcc
     1801 tccaccacgg cttcgtactg gtgcgcgccg gtcaccgcca atacgtcggg gaactttgcg
     1861 cggatcacct ccgcctcgtt gcccatgcac ccggtgacga tcacccgccc gttctcggca
     1921 atcgcctcgc cgatcgccgc caggctttcc tccttggccg aatcgaggaa cccgcaagtg
     1981 ttgaccagca ccacgtcggc cccggcgaag tcggggctca tcgcatagcc gtcggcgcgc
     2041 agccgcgtga ggatgcgctc ggaatcgacc agcgccttgg gacagcccag gctgaccatg
     2101 ccgaccttgc ggggggaggg aatttcaact gccatgggcg cgggcccata cacgccaaag
     2161 cctgaattgg ccattgccaa gcggcgcggg ccgtggccac tacggcccgc cttatctgga
     2221 gatcacgtca tgggaccggt aaattggctc gctgtcgccc tgtccactct ggtgatgctg
     2281 gcgctgtggg cggtctggcc cggcgcccgc cgcgcccggg tcaccgcgcc tggcatgctc
     2341 ggcgccctgg tcctcctcgc cctcccggcg gcgatgatcg gccacgcgct cgcccggatc
     2401 gggccggaga agctggccct gaaaccctgg ctctactgga tgcagacggg cggcgtggcg
     2461 gccttcttca tcatcccggc cctgtggctg gcccgcaccc gctccgcctg gaacggacgt
     2521 gaggcgctgg ccgaagccgg gttctggctc gtcgccttcc tcgccatggg cactgtgttc
     2581 tgggccacgg cctgaaccca aacccgccct ctcaatt
//
LOCUS       DATNBV010000129         4539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1050653,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000129 DATNBV010000000
VERSION     DATNBV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050653"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VN627_03100"
     CDS             <1..1084
                     /locus_tag="VN627_03100"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HXD02275.1"
                     /translation="VDRLMGGEVKRRADGGWPLDAIPKASGPLGPTPPRRPWSVRRTT
                     SIDTVWPGGIGTDLHMSGRGRDLLTGADPNHPEVLAEDSFTALLSRAREIHELDVTPV
                     RAGAAALVGSRAGSQLRSAIADALPGDLASASPLYLLLDDLAGASLVAAWAWSQWADD
                     WKDRLHSAAEAELKEPVHHKMIGVCVVFSPQSSMVLEGGPETQPDLNQPVPDLDNNGD
                     PFGWHQLAAQQGPSARRSRRIDVWEEGDVVRAVTHFQDSAPTPAGGRMAVHEYVVNAT
                     IDRATMTLTGIEADPRILPFGYCPAATLNVHELVGAPVGELRERVLAQFAKTQGCTHL
                     NDVLRSLAEVPMLAGRLERARAAAAG"
     gene            1207..1629
                     /gene="ndk"
                     /locus_tag="VN627_03105"
     CDS             1207..1629
                     /gene="ndk"
                     /locus_tag="VN627_03105"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXD02276.1"
                     /translation="MAATRTFSIIKPDATRRNLTGAVTKMLEEAGLRVVASKRIHMSR
                     EQAEGFYAVHKERPFFGELVEFMISGPVVVQVLEGEDAVKRNRDIMGATNPKDAAEGT
                     IRKSYAESIEANSVHGSDSDENAAIEIAFFFKPEEIVG"
     gene            1739..1993
                     /locus_tag="VN627_03110"
     CDS             1739..1993
                     /locus_tag="VN627_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02277.1"
                     /translation="MQGMGAADEACPFEFNFDPATFKVGDTVSYRVTGSLEGFPFVGT
                     LTEVHADFVLISPGDPTKPNESYRGTRESRPLVDESEIAL"
     gene            complement(2005..2583)
                     /gene="purN"
                     /locus_tag="VN627_03115"
                     /pseudo
     CDS             complement(2005..2583)
                     /gene="purN"
                     /locus_tag="VN627_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828416.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
     gene            complement(2704..3807)
                     /gene="purM"
                     /locus_tag="VN627_03120"
     CDS             complement(2704..3807)
                     /gene="purM"
                     /locus_tag="VN627_03120"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HXD02278.1"
                     /translation="MADEPEKQSYSYEQAGVSIAAGNALVKAIAPLARATARPGADAE
                     LGGFGGFFDLKAAGYKDPLLVAGNDGVGTKVKLAIDHDRHDAIGIDLVAMCVNDLIVQ
                     GAEPLLFLDYFATGKLDKGVAERVVAGIADGCKLAGCALIGGETAEMPGMYAAGDYDL
                     AGFCVGAVERGEQLTGDRVAAGDVLIGLASSGVHSNGYSLVRRLAADKGWKLDRPALF
                     DADRLLIDHLIEPTRIYVKSLLPLIRQNRIHALAHITGGGLLENVPRVLPKHLHARID
                     AGAWEQPRLMAFLQAQGNIEPEEMARTFNCGIGMVLAVAADEVAPLLADLAAAGETAH
                     VIGAIEDGQRGCTVQGSAEVWSARQDWEAVHLA"
     gene            3881..>4539
                     /locus_tag="VN627_03125"
     CDS             3881..>4539
                     /locus_tag="VN627_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HXD02279.1"
                     /translation="MAITLPPSTAPGARFRTPLLWGGGLLAAALLGAAVVAQIEGDRG
                     IAPVASTADIEVDGITVNTTGKTGDEARRNGWREATRQAWAKAGGPEMPDGQLEGLVS
                     AVVVQSEQIGPRRYIATLGVIFDRARAGQYIGGGDAAGLRSQPLLTIPVLYSGGAAQV
                     YEMRTPWQAAWAQFHAGASAIDYVRPNGGGGDSLLITAGQVGRRSRAWWRTVLDQFGA
                     S"
BASE COUNT          694 a         1451 c         1664 g          730 t
ORIGIN      
        1 ggttgatcgt ctgatggggg gcgaggtaaa gcgccgggcc gatggcggct ggccgctgga
       61 tgcgattccc aaggcgtccg gcccgctcgg gccgaccccg ccgcgccgcc cctggtcggt
      121 tcgccggacg acctcgatcg ataccgtctg gccgggcggc atcggcaccg acctgcacat
      181 gtcgggccgg ggccgcgacc tcttgaccgg agccgacccg aaccatcccg aggtgctggc
      241 cgaagacagt ttcacggccc tgctgtcgcg ggcgcgcgag atccatgagc ttgacgtgac
      301 accggtgcgg gcgggcgccg cggccctggt gggctcgcgg gcgggcagcc agctgcgctc
      361 ggcgattgcc gatgccctgc cgggagacct ggccagcgcc tcgccgcttt atctcctgct
      421 cgacgacctg gccggggcca gcctggtggc ggcctgggcc tggtcgcaat gggccgatga
      481 ttggaaggac cgcctgcaca gcgcggccga ggccgagctg aaggagccgg tgcaccacaa
      541 gatgatcggg gtctgcgttg tcttcagccc gcagtcgtcg atggttctcg aaggcggacc
      601 cgagacgcaa cccgacctca accagccggt ccccgacctg gacaacaacg gggatccgtt
      661 cggctggcac caactggcgg cccagcaggg gccatcggcg cggcgctcgc ggcgcatcga
      721 cgtgtgggag gagggcgacg ttgtgcgggc cgtcacccat ttccaggatt ccgctccgac
      781 gccggcgggc gggagaatgg cagttcacga atacgtggtg aacgccacaa tcgatcgggc
      841 caccatgacc ctgaccggga tcgaggccga tccgcgcatc ctgccgttcg gctattgtcc
      901 ggcggcgacg ctgaatgtcc acgagctggt cggggcgccg gtgggcgagc tgcgcgagcg
      961 cgttctggcc cagttcgcca agacgcaggg ctgcacccat ctcaatgatg tgctgcgctc
     1021 gctggccgag gtgccgatgc tggcgggccg gctcgagcgg gcgcgggcgg ccgccgccgg
     1081 ctgagcgcga aggggcggtg cgcgccgtgc ggcagggctt cggccccgcc gcgccttgcg
     1141 gtgcagcatt ttcccggcta ggggcgcgct ccattcggta atcacaaagt ctgcaaggaa
     1201 cacgaaatgg cggcgacccg caccttctcg atcatcaagc ccgacgcgac ccgtcgcaac
     1261 ctgaccggcg ccgtcaccaa gatgctggaa gaagccggcc tgcgcgtcgt cgcgagcaag
     1321 cgcatccaca tgagccgtga gcaggcggaa ggcttttatg ccgtccacaa ggagcgtccg
     1381 ttctttggtg aactcgtgga attcatgatc tcgggcccgg tcgtggtcca ggtgctcgag
     1441 ggcgaggatg cggtgaagcg caaccgcgac atcatgggcg ccaccaaccc caaggacgcc
     1501 gccgagggca cgatccgcaa gagctacgcg gaatcgatcg aggcgaattc ggtccacggt
     1561 tcggacagcg acgagaacgc ggccatcgaa atcgccttct tcttcaagcc ggaagagatc
     1621 gtcggctgaa cgccgggact gagtgtgatt ttcagggagc cgccggagcg atccggcggc
     1681 tcttttcatt gcgcggcggg cggcgcggga ctatcctttg cgccaaagga gatacgcaat
     1741 gcagggcatg ggcgccgcgg acgaggcctg tccgttcgag ttcaatttcg atccggcgac
     1801 cttcaaggtc ggcgatacgg tgagctatcg cgtgaccggc agccttgagg ggtttccttt
     1861 cgtcggcacg ctgaccgagg tccatgccga cttcgtgctg atctcgcccg gcgatccgac
     1921 caagcccaac gaatcctatc gcggtacgcg cgaaagccgg ccgctggtgg atgagagcga
     1981 aatcgcgctt taggttcaga cctgctggcg ctcgcgggtg accagcgcgg cgaggcagcg
     2041 gggatacagc tggtgttcgg cgatcaggac gcgggcggcc aggctttccg cagtgtcgcc
     2101 cggcaggatc gcgaccgggg tctggcccag catggggcct tcgtcgagtt cggtggtgac
     2161 gaggtggacg gtgcagccgc cgtggctgtc gcctgcgtcg agcgcccgct ggtgggtctg
     2221 tagcccggga tatttcggca ggagcgaggg gtggatgttg accatccggc cggcccagcc
     2281 gctcacgaat tcgggcgtca ggatacgcat gtagccggcg agcgcgacat attgcgcgcc
     2341 gcggctgcgg atcgcggcgt ccatcgcgcg gtcgtgctcg gcccgggtca tgcccttgtg
     2401 cgacagggcg aaggtgggaa tcccttcggc ttcggccagg ccgagccctt tcgccgcacg
     2461 atcgttggcg gcgacgagca ccagctcgta ggggcaatcg gccgcgcggc tgccgtagag
     2521 cagggcggcc atgttggtgc ccgtgccgga catgagaacg gcgaccttgg cgcgggtggt
     2581 catgctgggg cttccctgcg tcagaagcgg tcgcgcgagt gggagacgtg gttggcccct
     2641 ccgtcaggcg cctgccagcg cctgccacct ccccatttgt gcttcgcaaa aacggggagg
     2701 aatttacgca aggtgcacgg cttcccagtc ttgccgcgcg gaccagactt cggccgaccc
     2761 ttgcacggtg cagccgcgct ggccgtcttc gatggcgccg atgacgtggg ccgtttcgcc
     2821 ggcagcggcg aggtcagcca gcaagggggc aacttcgtcc gctgctacgg caaggaccat
     2881 gccgatgccg cagttgaagg tgcgggccat ctcctcgggc tcgatattgc cctgggcctg
     2941 gaggaaggcc atcagccgcg gctgctccca ggcgccggca tcgatccggg cgtggaggtg
     3001 cttcgggagg acgcgcggta cgttttcaag caggccgccg ccggtgatgt gggcgagggc
     3061 gtgaatgcga ttctggcgga tcagcgggag caggctcttc acgtagatgc gggtgggttc
     3121 gatcaggtga tcgatcagca gccggtcggc atcgaacagg gcagggcgat cgagcttcca
     3181 ccccttgtcg gcggcgaggc ggcgcacgag cgaatagccg ttggagtgga cgcccgagga
     3241 ggcgaggccg atcaggacgt caccagcggc gacgcggtca cccgtcaact gctcaccgcg
     3301 ttcgacggcg ccgacgcaga agccggcgag atcatagtcc cccgcggcgt acatgccggg
     3361 catttcggcg gtttcgccgc cgatcagggc gcagccggcg agcttgcagc catcggcgat
     3421 gccggcgacc acgcgctcgg ccacgccctt gtccagcttg ccggtggcga aataatcgag
     3481 gaacagcagc ggctcggcgc cttggacgat gagatcgttg acgcacatgg cgacgagatc
     3541 gatgccgatc gcatcgtgcc ggtcatgatc gatggcgagc ttgaccttgg tgcccacgcc
     3601 atcgttgccg gcgactagca gcgggtcctt gtagccagcc gccttgaggt cgaaaaagcc
     3661 gccgaatccg ccgagttcgg catcggcgcc gggccgcgcg gtggcgcggg cgagcggcgc
     3721 gatggccttc accagggcgt tgccggcggc gatggagacg ccggcctgtt cataggagta
     3781 ggactgcttt tcgggttcgt cggccatatc gcagcgcgcc taccgctttc gcgcttggat
     3841 ttccacttcg cattgggcaa aaggccgcca gtttcccccg atggctatca cgctcccccc
     3901 cagcaccgcg cccggcgccc gtttccgcac cccgctgttg tggggtggag ggctgttggc
     3961 ggctgccctg ctgggcgcgg cggtggtggc gcagatcgag ggggaccggg gaatcgcccc
     4021 ggtcgcctcg acggctgaca ttgaggtgga cggcatcacc gtcaacacca ccggcaagac
     4081 cggcgacgag gcccgccgca acggctggcg cgaggcgacg cggcaggcct gggccaaggc
     4141 cggcgggccg gaaatgcccg atggccagct ggaggggttg gtttcggcgg tggtggtgca
     4201 gagcgagcag atcggcccgc gccgctatat cgccacgctg ggcgtgatct tcgaccgggc
     4261 ccgcgcgggg cagtatatcg gcggcggcga cgccgcgggc ctgcgctcgc agccgctgct
     4321 gacgatcccg gtgctctatt cgggcggggc ggcgcaggtg tatgagatgc gtacgccgtg
     4381 gcaggcggcc tgggcccagt ttcacgccgg ggcgagcgcg atcgactatg tccggcccaa
     4441 tggcggcggg ggcgattcgc tgctgatcac cgccgggcag gtgggccgcc gttcgcgggc
     4501 ctggtggcgc acggtgctcg accagttcgg cgcgagcga
//
LOCUS       DATNBV010000130         2057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_101819,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000130 DATNBV010000000
VERSION     DATNBV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101819"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VN627_03130"
     CDS             complement(<1..952)
                     /locus_tag="VN627_03130"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXD02280.1"
                     /translation="MFVGLDTSKLKISVALAEEGRQGEVRFLGEIDNTPEAVRRLVGK
                     LASRYSEVLFCYEAGPTGYGLQRQIAALGHECDVIAPSLIPKRPGERVKTNRRDALTL
                     ARLHRAGELTAIWVPDPGHEAVRELVRARDAAMADLRAKRQHLQSFLLRHGRLFPGRK
                     PWTRAHARWLSELAFEHPAQYLVLREYRQGIEDAQARLERLTQQIADVVTTWSMAPAV
                     EAYQALRGVALVTAVTFVAEIGDVRRFESPRQLMAYLGLVPCESSTGERVRRGSITKA
                     GNTRVRRVLIEGAWTYRFPARMSRLLQERQAKLPQIVRTIA"
     gene            1063..1881
                     /locus_tag="VN627_03135"
                     /pseudo
     CDS             1063..1881
                     /locus_tag="VN627_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553139.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
BASE COUNT          342 a          714 c          668 g          333 t
ORIGIN      
        1 aggcaatcgt ccgcacaatc tgcggcagtt tcgcctggcg ctcctggagc agtcggctca
       61 tccgggccgg aaagcgatag gtccaagccc cctcgatcag cacgcgccgt actctggtgt
      121 tgccggcctt ggtgatgctg ccccgccgca cccgctcgcc ggtcgagctc tcgcacggca
      181 ccagacccaa ataggccatc agctgccgcg ggctctcgaa ccggcgcaca tcgccaatct
      241 ccgccacaaa ggtcacggcg gtgacaagcg cgacaccgcg gagggcctga taagcctcga
      301 ccgcaggagc catcgaccaa gtggtgacca catcggcgat ttgctgcgtc aggcgctcca
      361 gacgggcctg cgcatcctca atcccctgtc ggtattcccg caggaccaaa tactgggccg
      421 gatgctcgaa ggcgagttcg gacaaccacc gcgcatgggc ccgtgtccag ggcttgcggc
      481 cgggaaagag ccgcccatgg cgcaacagaa acgactgcag atgctggcgc ttcgcgcgca
      541 gatccgccat cgcggcgtcc cgcgcccgca ccagttcgcg gaccgcctca tggcccggat
      601 ccggcaccca gatggcggtc agctcgccgg cccggtggag ccgggccagg gtcagggcat
      661 cccggcggtt ggtcttcacg cgctcgccgg ggcgcttcgg gatcagggag ggcgcgatga
      721 cgtcacactc atgcccgagc gccgcgatct ggcgctgcaa gccatagccg gtcggacccg
      781 cctcgtagca gaacagcact tcgctgtatc ggctggccag cttgccgacg aggcgccgca
      841 ccgcctctgg cgtgttgtcg atctcgccga ggaagcggac ctcaccctgg cggccttcct
      901 cggccaaggc gaccgagatc ttcagcttgg aggtatccag accgacaaac actctgcttc
      961 tctgcggcat ggctcgtccc ttgtgcggga ggcgcggcgc cggcccatcc ggaacaaccc
     1021 tcgctcgtcc catagcgggg acgagccacc tccgccggca acgaacatac ggtctgtcag
     1081 atcggcgtgt tcgccgccta tgtgtcgggc aaagggcacg ccttcatcga ccgggccctc
     1141 tatcttcctc aggactggac caagaacccc gcccgcctgc gtgcagcgca cgtgccggag
     1201 gaggtcgggt ttacaaccaa gccccggctt gcgctcgata tgatcgaggg cgccatcaac
     1261 gcgggggtgc cctttgggtg ggtggccgcc gacacggtct acggcacggg tgaggtcgag
     1321 ctggcgctgc gccgcttcgg cagaggatac gtgctcgggg ccggcgccac gcagctcttc
     1381 aactcctgga tcggcaagcc ggaggtggcc ggcacggccg aagcgatcgc gcgagacttg
     1441 cagccttcgg cgtgggtgcg cctgtccgcg ggcgaaggca ccaaaggacc tcggctgtat
     1501 gactgggctt acttggaact ggccgacctc gaagcagacg cgttcaaccc cgccctggtc
     1561 gggacctgga cgcggggcct gttgatccgc cgcaacctca ctggcggaga gcttgccttc
     1621 ttctcgacgt ggtgcccggc cgggacagcg atccagacgc ttgtggccgt ggaggggcgc
     1681 cgctgggcca tcgaggacgc gttcgagacc gccaagaccg aactcgggct tgctcacaac
     1741 gagacccgct cctggcacgg ctggcaccgg catgtctctc tggtcatgct cgcctttgcc
     1801 atgatgagtg cagtccggta tcgcgcgaac cacctgccgc ccctaaaaac gaagggcagg
     1861 gctcagcggg ctgcctcctg atccgctggt cggtccagga gatccggcgt gtgacgatgc
     1921 gcttggccca gcgtcgcatc cgcccagccc atgtgattgc ttggtctctg tggcgccgcg
     1981 cccaccaggc cgcggcacaa cacgctcaca tcaaacgcag accgcaactg taatgctagg
     2041 tatgggctgt gctccgg
//
LOCUS       DATNBV010000131         3046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2307412,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000131 DATNBV010000000
VERSION     DATNBV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2307412"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..981)
                     /locus_tag="VN627_03140"
     CDS             complement(112..981)
                     /locus_tag="VN627_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HXD02281.1"
                     /translation="MIAPDRPRRSVLYLPASNARAIEKARSLPADVIVLDLEDAVAPE
                     AKAEARAAAVAAVRAGGFGRRELAIRANALDTPWGADDLAAIAASGADAVVMPKVSRP
                     ADIAACQSALAAAPAGLQLWAMIETCAAVGALFDLAAMAADTRLSLWMLGTNDLAKEM
                     RARLTPDRTAFLPILTQAICAARAHGLAILDGVCNEFRDLDLFRAEAEQGLLLGFDGK
                     TLIHPDQVAPCNAVFSPSAEELARAQAIIAAFALPEYAGKGVIRVDGKMAELLHLEQA
                     QRLIAIAEQIAAS"
     gene            complement(994..1476)
                     /locus_tag="VN627_03145"
     CDS             complement(994..1476)
                     /locus_tag="VN627_03145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXD02282.1"
                     /translation="MEIEAATFRQVLGHYPTGVCVVTALDKDGNPCGMVVGSFTSVSL
                     DPPLVGFFPDKRSGSWPLIEEAGHFCVNVLASDQTDLCRQLARPGANKFTDVEYELSA
                     HNLPILAGATVAIECRLESVVPAGDHWLVLGRVLGLEARRDAGPMLFFRGQYGGFSSL
                     "
     gene            1624..2160
                     /locus_tag="VN627_03150"
     CDS             1624..2160
                     /locus_tag="VN627_03150"
                     /inference="COORDINATES: protein motif:HMM:NF022115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2478 domain-containing protein"
                     /protein_id="HXD02283.1"
                     /translation="MSRNASPIAVLQGASSEAIQGLLADFAARVAEAGGVRIAGLIEC
                     APEGCHGDAPALLRTIAGRHTFPIFQDLGSGSTACALDSVSLVAAGEQVRADIAAGCD
                     LVVLSKFAKLEAENRSGLLAAFGDAIAAGLPIVTAVSPKFMDRWDAFAAPLYACLPAD
                     AAALDAWWQGQGPIKTND"
     gene            2269..>3046
                     /locus_tag="VN627_03155"
     CDS             2269..>3046
                     /locus_tag="VN627_03155"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXD02284.1"
                     /translation="MKLEAEKIIEQAVRQTGLDDFGGETFRTGLDLLVKGINRDTGRT
                     PEVFAMNEGMFVKTLADRLQVIQALKDRPEVLDEEVKRPVFVFGLPRTGTTLLCNLLS
                     VDPARRSTIRWELDSPVPPPTTATLRTDPRALAAIKAEEEAMAANPEGGKYNRWGPLE
                     PFECVSILAHEFNTLIFESMGELPEYGEYVMNSDWTPGYEYHRKFLQLHQIDAPGVWN
                     LKMPSHIFALPQLLKVYPDARLIWTHRDPYAAAGSLCSLIA"
BASE COUNT          525 a          927 c         1097 g          497 t
ORIGIN      
        1 tacatgcccg acctgctgat ctgccggaag gaacatgcgc agatggtgct ggccgaggtg
       61 gccaaggtcg acgcctgaac tgattgatcc tccccgcttg cggggaggaa ttcagctggc
      121 cgcaatctgc tcggcgattg caatcaggcg ctgcgcctgc tcgaggtgga gcagttcggc
      181 catcttgccg tcgacccgga tgacgccctt gccggcgtat tccgggaggg cgaaggcggc
      241 gatgatcgcc tgggcgcggg ccagttcctc ggccgagggg gagaagaccg cgttgcaggg
      301 ggcgacctga tccgggtgga tcagggtctt gccgtcaaag cccagcagga ggccctgctc
      361 ggcctcggcc cggaaaaggt ccagatcgcg gaattcgttg cagacgccgt cgaggatggc
      421 gaggccgtga gcgcgggcgg cgcagatcgc ctgggtgagg atcggcagga aagccgtgcg
      481 gtccggcgtg aggcgggcgc gcatttcctt ggcgagatcg ttggtgccca gcatccacag
      541 cgacaaccgc gtatcggcgg ccatggcggc gagatcgaac agggcgccga cggcggcgca
      601 ggtttcgatc atcgcccaga gctggagccc ggcgggggcg gcggcgagcg cagactggca
      661 ggcggcaata tcagccgggc gcgacacctt gggcatgacc acggcatcgg cgccggacgc
      721 ggcgatggcg gcgagatcgt ctgcccccca gggtgtatcg agggcattgg cgcggatcgc
      781 gagttcgcgc cggccgaacc cgccagcccg gaccgccgcg acggcagcgg cgcgggcttc
      841 ggccttggct tcgggcgcca cggcatcttc gagatcgagc acgatcacat cggctggcag
      901 gctccgcgcc ttttcgatgg cccgggcatt ggacgcgggc agatagagca cggagcggcg
      961 ggggcggtca ggcgcgatca tcggacgtgg ctttcagagc gaggagaaac cgccgtactg
     1021 gccgcggaaa aacagcatcg gaccggcatc gcgccgcgct tccagaccga gcacccggcc
     1081 cagcaccagc cagtgatcgc cggctgggac gacagattcc aggcggcatt cgatcgcgac
     1141 cgtcgcgccg gcgaggatgg gcaggttgtg agcggacaat tcgtattcca cgtcggtgaa
     1201 cttgttcgcc ccggggcggg cgagctggcg gcacagatcg gtctggtcgc tggcgaggac
     1261 gttgacgcag aaatgcccgg cctcctcgat caaaggccac gagccagaac gcttgtcggg
     1321 aaagaagccg accagtggcg ggtcgagcga gaccgaggtg aacgagccca cgaccatccc
     1381 gcaggggttg ccgtccttat caagcgcggt gaccacgcag acgccggtag gatagtggcc
     1441 gagcacctga cggaaggttg ccgcctcgat ttccatttcg cccactgtgg tctcccgtaa
     1501 cttcatgact gccatgggca atgcggcggc cccgcgcaag agggcggagg gggcaggcga
     1561 gccgattagg cctgaatggc ctgcgttgtg gacagcatcg gacgaggcgg gcagggtgcg
     1621 cggatgagca gaaatgccag ccccattgcc gtgcttcagg gcgcctcgag cgaggcgata
     1681 caggggctgc tggcagactt cgcggcccgg gtagccgagg ccggcggggt gcggatcgcc
     1741 gggttgatcg agtgtgcgcc ggaggggtgc catggcgatg ccccggccct gctgcggacc
     1801 attgccggac gtcacacctt tccgatcttc caggatctgg gctcgggctc cacggcctgt
     1861 gcgcttgatt cggtttcgct ggtggcggca ggggagcagg tgcgggccga tatcgccgcc
     1921 gggtgcgatc tcgtggtgct gagcaagttt gccaagctgg aggcggagaa ccggtccggg
     1981 ctgctggcgg cgtttggcga tgcgatcgca gcggggctgc cgatcgttac cgcggtatcg
     2041 cccaaattca tggaccggtg ggatgccttc gccgcgccgc tttatgcctg cctcccggcc
     2101 gatgcggcgg cgcttgacgc gtggtggcag ggacaagggc cgatcaagac aaatgactga
     2161 cattcatgtg atcggttgct ctcggcgatt gctctcgagc actggggtag acatgcgtct
     2221 agcgatcgac tgccggattt gaccgagtcg agctgtgtgg agagttggat gaagctggaa
     2281 gccgaaaaga tcatcgaaca ggctgttcgc cagaccgggt tggatgactt cggcggggaa
     2341 accttccgca cgggactgga cctgctggtg aagggcatca accgcgatac cgggcgcacg
     2401 cccgaggtct tcgccatgaa cgaggggatg ttcgtcaaga ccctggccga ccggctgcag
     2461 gtcatccagg cgctcaagga ccggcccgag gttctggacg aggaggtgaa gcgcccggtc
     2521 ttcgtgttcg gcctgccgcg cacggggacg accctgctgt gcaacctgct ctcggtcgat
     2581 ccggcgcgac gttcaaccat acgctgggag ctcgacagcc cggtcccgcc gccgaccacg
     2641 gccacgcttc gcaccgatcc gcgcgcgctg gcggcgatca aggccgagga ggaggccatg
     2701 gcggccaacc cggaaggcgg caagtataac cgctggggcc cgcttgagcc gttcgagtgc
     2761 gtttcgatcc tggcccatga attcaatacg ctgatcttcg aatcgatggg cgagttgccg
     2821 gaatacggcg aatacgtgat gaattcggac tggacccccg gctacgaata tcatcgcaag
     2881 tttctccagc tgcaccagat cgatgcgccg ggcgtgtgga acctgaagat gccgagccac
     2941 atcttcgccc tgccgcagct gctcaaggtc taccccgatg cgcggctgat ctggacccac
     3001 cgcgatccct atgccgccgc cggttcactg tgcagcctga tcgcgg
//
LOCUS       DATNBV010000132         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4563375,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000132 DATNBV010000000
VERSION     DATNBV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4563375"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VN627_03160"
     CDS             <1..924
                     /locus_tag="VN627_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HXD02285.1"
                     /translation="KVGDLLVPMPINRTTLNALYGLRLDTEEEAAAFLASRAEPVDVV
                     ETSEDVVISAVGRELYETFFRGYTRKQWGLDPSELDKSVTSRVPTRTNVDDRYFTDKF
                     QAMPADGFTAMFEKMLDHPNIDLLLGVDYAEARAAYPHAHLVFTGPIDEYFGYRYGRL
                     PYRSLRFEHETLDQEWLQPVAVVNYPDEAVPFTRITEYKHLTGQAHPRTSITREYPQA
                     EGDPYYPVPREENQALYKRYEALARAEDGVTFVGRLATYRYYNMDQIVGQALATFRKA
                     FGEERRSRALVTARAPSASSGAAAGAVASAA"
     gene            933..2081
                     /gene="dgoD"
                     /locus_tag="VN627_03165"
     CDS             933..2081
                     /gene="dgoD"
                     /locus_tag="VN627_03165"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HXD02286.1"
                     /translation="MPALRAIETFAVPPRWLFVRVETDAGEVGWGEASLEGHAEAVAG
                     AFAALRDRFIGHDADAIEDIWQVAHRLGFYRGGPVLMSALSGLDQALWDLKGKRLGVP
                     VWQLLGGQVRQKIPVYAWIGGDRPHDVAEAARTRRDQGFHAVKMNATEDIGWLDSPRV
                     LEATVARLEAVQGQGMEAGLDFHGRVHRAMARQLARALEPLHPLFIEEPVLSEHPEAL
                     AQLAAQTSVPIALGERLYSRWDFKPFFEASCVDIVQPDLSHAGGISECRRIAAMAEAY
                     DVALAPHCPLGPIALAACLQVATTSINFVIQEMSLGIHYNDADHDLLTYVADPAPFAV
                     SDGTVPVPQGPGLGVEIDEAKVREAAGRMTKGWRNPVWRGPDGAVREW"
BASE COUNT          365 a          731 c          790 g          373 t
ORIGIN      
        1 aaggtgggtg acctgctcgt gccgatgccg atcaaccgga cgacgcttaa cgcgctatat
       61 gggttgcggc tggatacgga ggaggaggcc gctgcgttcc tcgcgtcgcg cgccgagccg
      121 gtcgacgtgg tggagacatc cgaggacgtg gtgatctcgg ccgttgggcg cgaactctac
      181 gagaccttct ttcgcggcta tacccgcaag cagtgggggc tcgatccaag cgagctggac
      241 aagtccgtca cctcgcgggt cccgacgcgg accaatgtgg acgaccgcta tttcaccgac
      301 aagttccagg ccatgcccgc cgatgggttc acggcaatgt tcgagaagat gctcgaccat
      361 ccgaacatcg acctgttgct gggggtggac tatgccgagg cgcgggcggc ctatccccat
      421 gcccacctcg tttttaccgg cccgatcgac gagtatttcg gttatcgtta cggcaggctg
      481 ccctaccgtt cgctgcgctt cgagcacgag acgctcgatc aggaatggct ccagcccgtc
      541 gcagtggtaa actatccgga cgaggccgtt ccgtttacgc ggatcaccga gtacaagcac
      601 ctcaccgggc aggcgcaccc gcgcaccagc atcacccgcg aatatccgca ggcggagggc
      661 gatccctact atcccgtccc gcgcgaggag aatcaggcgc tctacaagcg ctacgaggcg
      721 ctggcgcggg ccgaggacgg ggtaaccttc gtcggccgac tggcgactta ccgctattac
      781 aatatggacc agatcgtcgg tcaggcattg gcgaccttcc gcaaggcctt cggtgaagag
      841 cggcggagcc gggccttggt gacggcgcgg gcgccgagcg cttcgtcggg cgcagcggcg
      901 ggcgcggtcg cttcggcggc ctgagcggcg ccatgccggc gctgcgggca atagagacct
      961 tcgcggtccc cccgcgctgg ctgttcgtcc gcgtcgagac cgacgcgggt gaggtcggct
     1021 ggggcgaggc aagcctcgaa ggccatgccg aagccgtggc aggtgccttc gcggccctgc
     1081 gcgaccggtt catcggccac gatgccgacg ccatcgagga tatctggcag gtcgcccacc
     1141 gcctcgggtt ctaccggggc ggaccagtgc tgatgagcgc gctctccggg ctggaccagg
     1201 cgctgtggga tctcaagggc aagcggctgg gcgttccggt gtggcagttg ctgggcgggc
     1261 aggtgcggca gaagattccg gtctatgcct ggatcggcgg cgaccggccg cacgacgtcg
     1321 ccgaggccgc gcgtacccgg cgggaccagg gcttccacgc ggtcaagatg aacgccaccg
     1381 aggacatcgg ctggctcgac agcccccgcg tgctggaggc gaccgtggcg cggctggagg
     1441 cggtgcaggg ccagggcatg gaagccgggc tcgatttcca tggccgggtg caccgggcca
     1501 tggcgcgcca gctcgcccgt gcgcttgagc cgctgcaccc gctgttcatc gaggagccgg
     1561 tgctgtcgga acaccctgag gcactcgccc aactggccgc ccagaccagc gtgccgatcg
     1621 cgctcggcga gcggctctac agccggtggg acttcaagcc ctttttcgag gcgagctgcg
     1681 tcgacatcgt ccagcccgat ctcagccatg ccggcggcat ttccgaatgc cgccgcatcg
     1741 ccgccatggc cgaggcctat gacgtggcgc tcgccccgca ttgcccgctg ggaccgatcg
     1801 cgctggcggc ctgcctgcag gtggcgacga cgagcatcaa cttcgtaatc caggagatgt
     1861 cgctcggcat ccactataac gatgccgacc atgatctgct gacctatgtg gccgacccgg
     1921 cgccgtttgc cgtgagcgac ggcacggtgc ccgtgccgca ggggcctggc ctcggggtgg
     1981 agatcgacga ggccaaggtc cgcgaagctg ccgggcgcat gaccaagggc tggcgcaatc
     2041 cggtgtggcg cgggccggat ggggctgtgc gcgagtggtg aggggcaagt cacgtgatgc
     2101 cttggcctgc ccgtcgtcct gaacttgctt caggatccat ggcgcaagga tggcgcactg
     2161 tcggacatgg ctgacttggc gcgaccgtcg gtccatggat cctgaagcaa gttcaggacg
     2221 acgggcaggc caaggcatca cgtgacttgc ccctcacca
//
LOCUS       DATNBV010000133         7053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1052756,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000133 DATNBV010000000
VERSION     DATNBV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052756"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VN627_03170"
     CDS             complement(<1..410)
                     /locus_tag="VN627_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXD02287.1"
                     /translation="MTDNLTPKAIVRALDEHIIGQAEAKKAVAVALRNRWRRQRLSPE
                     LRDEVTPKNILMIGPTGCGKTEISRRLAKLAEAPFVKVEATKFTEVGYVGRDVDQIIR
                     DLVESALAMVREKRRAGVKAKAESAAEERILDALV"
     gene            complement(542..1102)
                     /gene="hslV"
                     /locus_tag="VN627_03175"
     CDS             complement(542..1102)
                     /gene="hslV"
                     /locus_tag="VN627_03175"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HXD02288.1"
                     /translation="MDDNRGSHGLIQWHGTTIIGVKKNGRTVIAGDGQVSMGNTVMKP
                     NARKVRRIGEGGKVIAGFAGATADAFTLFERLERKLEQYRGQLMRAAVELAKDWRTDK
                     YLRNLEALMIVADAETMLVLTGNGDVLEPEGGITAIGSGGNYALAAARALDAYEDDAE
                     TIARKAMQVAADICVFTNDRVTVETI"
     gene            complement(1223..1720)
                     /gene="bamE"
                     /locus_tag="VN627_03180"
     CDS             complement(1223..1720)
                     /gene="bamE"
                     /locus_tag="VN627_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HXD02289.1"
                     /translation="MRVNKRLVIGGAALAAGLVALSGCSSIKDHRGYLVDQALVDSVQ
                     PGIDNRASVERTLGRPTYVAQFGEPVWYYVSIDTKQAAFTRPSAYKETVLKVHFDPTG
                     NVSRVDRTGIEKVVRLDPDHHKTPTLGRNRGFLEDLFGNIGTVGAPGMGGGGGGEGGG
                     GPNGS"
     gene            1870..2391
                     /locus_tag="VN627_03185"
     CDS             1870..2391
                     /locus_tag="VN627_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HXD02290.1"
                     /translation="MSFFKRLLRPRGDDREPVRPLWHRTVELARDPRWYAEGGVADTV
                     AGRFDAITLVLCAVLLRMEREPALVAPSVLLTELFVDDMDGQLRQSGVGDLVVGKHIG
                     KLVSSLGGRLGALREALGEPGDEALVAAVNRNVSLGDKGNPPRVAALLSGFAGALDAA
                     SAEDLLAARIALP"
     gene            2388..2906
                     /locus_tag="VN627_03190"
     CDS             2388..2906
                     /locus_tag="VN627_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HXD02291.1"
                     /translation="MSAPEFSRMVDLRQITAAPVQLSANEGERKALAGRFGLVSLDRL
                     EATVSLEADGPVVSAKGRISAVWVQPCAVSGEDLAQSADEEIDLRFVPARTDHAPDEE
                     IELSEDELDEIEYTGTSFDLGEAVAQSLALAIDPFASGPEAEAARKAAGIVNEGQAGA
                     FAALAGLKLPKD"
     gene            complement(3009..3575)
                     /gene="ssb"
                     /locus_tag="VN627_03195"
     CDS             complement(3009..3575)
                     /gene="ssb"
                     /locus_tag="VN627_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXD02292.1"
                     /translation="MAGSVNKVILIGNLGQDPEVKSFQNGGRIANLRIATSESWKDRN
                     TGERKERTEWHSVVLQSDGLVGVAERYLRKGSKVYIEGQLRTRKWQDASGNDRYTTEI
                     SVGPGGSLTMLDGSGGAGREGGSGAGASGGRSWNEGGSSGGGYGQGSSSGRAGGGSSG
                     GWNQGGGGAGGTGGAGGFADDLDDDIPF"
     gene            complement(3677..5530)
                     /locus_tag="VN627_03200"
     CDS             complement(3677..5530)
                     /locus_tag="VN627_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HXD02293.1"
                     /translation="MSRQRSAALVGNPNAGKSALFNALTGARQKIANYPGVTVERKSG
                     RLVLPSGEPVELTDLPGSYGLHPTSPDEEVTAKVIMGRFPGEAAPEVLVVVLDASNLE
                     QHLVFAQEVIALGRPTVVALNMIDLAERDGLVLDPQALEQALGVPVVPTVAVRRRGLD
                     ELAAAIASAEARAAEPNPGHPRPHIDLTERRISAHAIAEAAIVSETRRHRLHAKLDKL
                     LLHPWIGPVILFALLFVVFQAVFAGATPFADALDGAVSWLGDQVRAAMPPSLARDLLT
                     DGIISGVGSVVVFLPQIVILFFFILAMEASGYMARAAFLMDRMMAGVGLSGRSFIPLL
                     SSFACAIPGIMATRSITDPKDRLTTILIAPMMTCSARLPVYAVIIAAFIPNRTVGWGV
                     GLQGLVLLALYVAGIVGAMAVALILRRSVTKGAASGFIMELPKYQMPRVKDMAIGLWQ
                     RAWIFLRRAGTIIFTVTVVLWLLLNFPRAQDGQNQVDASLAGHIANGLAVVVEPIGFN
                     RDMALSLIPAMAAREVAVSSLATTYAVAAADDAAGSQQLGDRLRGKWTLPMALAFLAW
                     FVFAPQCMSTIAVTRRETNGWKWPAFMLAYLFGLAYVAAGVTYWSAVALGL"
     gene            complement(5527..5799)
                     /locus_tag="VN627_03205"
     CDS             complement(5527..5799)
                     /locus_tag="VN627_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HXD02294.1"
                     /translation="MTLDQLPIGKRARIVAVDWSRLVDEEGQRLRALGIDTGARVALA
                     HRGVFGGRDPLAVMVGRMTVALRRVHAAAMEVEPVAETAGANGAAA"
     gene            complement(5970..6626)
                     /locus_tag="VN627_03210"
     CDS             complement(5970..6626)
                     /locus_tag="VN627_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HXD02295.1"
                     /translation="MDYSDLTLDELRLALAPAIADAAIFDGWSKNAVTNAALAHGVKP
                     EVARIAFPGGAMDMIAAWIARIDADMAAALPAERLGNLPIRERIRSLVQFRLDAVAGR
                     EEALRRALAIMAMPQNAARTAKLSWSSADRMWRLAGDTAADFNHYTKRATLAALYGAT
                     LLAFVADESEGKAETRAFLDRRIAGVMRFEKAKARLLRPREDSFSITRLLGRLRYPAN
                     "
     gene            complement(6637..>7053)
                     /locus_tag="VN627_03215"
     CDS             complement(6637..>7053)
                     /locus_tag="VN627_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXD02296.1"
                     /translation="ETALHIVTQRRDLTWLSFLLAKGANPNLRTDKGVTPLAVAVALD
                     WADGADLLLDQGARPNDPDGTGETPLIAAVHQHSVELVRLLIKAGADPLRADNSGRNA
                     RDYAELQGKDSAIWGAIDAAATAAAAKRKQSYGPSF"
BASE COUNT         1094 a         2368 c         2484 g         1107 t
ORIGIN      
        1 accagggcgt cgaggatgcg ctcctcagcg gcgctctcgg ccttggcctt gaccccggcg
       61 cggcgcttct cgcgcaccat cgccagcgcg ctttcgacca ggtcgcggat gatctggtcg
      121 acgtcgcggc cgacgtagcc gacctcggtg aacttggtcg cctcgacctt gacgaacggc
      181 gcctcggcca gcttggccag gcggcggctg atctcggtct tgccgcagcc ggtgggcccg
      241 atcatcagga tgttcttggg ggtgacctcg tcgcgcagct cggggctgag gcgctgccgg
      301 cgccaccggt tgcgcagggc cacggcgacg gccttcttgg cctcggcctg gccgatgatg
      361 tgctcgtcca gcgcgcggac gatggccttg ggggtgagat tgtcggtcat tctttcctct
      421 tacgtcatcc cggacttgat ccgggaccgc tgtcggacgc gaagctgtga gggcgcctga
      481 aggcagatcc acagcttcgc caaggccacc ggtcccggat caagtccggg atgacgcctg
      541 ttcagatcgt ttccaccgtg acgcggtcgt tggtgaacac gcagatgtcc gccgccacct
      601 gcatagcctt gcgggcgatg gtttcggcgt catcctcata ggcatcgagc gcgcgggcgg
      661 cggcgagggc atagttgccg cccgagccga tggcagtgat cccgccttcc ggttccagca
      721 cgtcgccatt gccggtgagg accagcatgg tctcggcatc ggcgacgatc atcagcgcct
      781 cgagattgcg caggtacttg tcggtccgcc agtccttggc aagttcgacc gcggcgcgca
      841 tgagctggcc gcgatactgc tcgagcttgc gctccagccg ctcgaacagg gtgaaggcat
      901 ccgccgtcgc cccggcgaac ccggcgatga cctttccgcc ctcgccgatg cgccgcacct
      961 tgcgggcatt gggcttcatc accgtattgc ccatggaaac ctggccatcg ccggcaatca
     1021 ccgtgcggcc gttcttcttc accccgatga tggtggtgcc gtgccactgg atgaggccgt
     1081 ggctgcccct gttgtcgtcc atgccccggc atatggggaa ccacggcgca ggatcaagaa
     1141 ggtttgaccg agtgcggaaa gccgatccgg gcttccgttt gcggcaccag ccctccacgg
     1201 acgtggaggg ggcaccctca aatcagctcc cgttggggcc gccgccgcct tcgccgccgc
     1261 cgccgccacc catgccgggc gcgccgaccg tgccgatgtt gccgaacagg tcttcaagga
     1321 agccacgatt gcggccgagg gttggcgtct tgtgatgatc gggatcgagc cggacgacct
     1381 tttcgatacc ggtgcgatcg acccggctga cgttgccggt gggatcgaag tgcaccttca
     1441 gcacggtttc cttgtaggcg ctcgggcggg tgaaggcggc ctgcttggtg tcgatcgaga
     1501 catagtacca caccggctca ccgaactggg cgacataggt cggccggccg agggtgcgtt
     1561 cgaccgaggc gcggttgtca atcccgggct ggaccgaatc gaccagcgcc tgatccacga
     1621 gatagccgcg atggtccttg atcgaggaac agcccgacag cgccacgagc ccggccgcga
     1681 gcgccgctcc gccaatcacc agccgcttgt tgacccgcat gtagactccg cttctctgcc
     1741 ttcaggcgcc ggttgccaag cgcccggacc cgcctatacc gcgcaagcct taggctgcgc
     1801 gggccaaacc cgcaaggacc gtggccgccg cggattgaat ccaccctgaa taaccgaaag
     1861 ccacccccga tgtcgttttt caagcgcctg ttgagacccc gcggggatga ccgcgagccg
     1921 gtccgcccgc tgtggcaccg cacggtcgaa ctggcccgtg atccgcgctg gtatgctgaa
     1981 ggcggggtgg ccgatacagt ggccgggcgg ttcgatgcga tcacgctcgt cctctgcgcg
     2041 gtcctgctgc ggatggagcg cgagccggcg ctggtggccc cctcggtgct gctgaccgag
     2101 ctgttcgtcg acgacatgga cggccagctg cgccagtccg gcgtgggcga cctcgtggtg
     2161 ggcaagcaca tcggcaagct ggtaagctca ctgggcgggc ggctcggcgc cctgcgcgag
     2221 gcgttgggcg agcccggcga cgaggcgctg gtggcggcgg tgaaccgcaa cgtttcgctg
     2281 ggagacaagg gcaatccgcc cagggtggcg gcgctgctga gcggctttgc cggcgcgctc
     2341 gatgccgctt cggccgagga cctgctggcc gcgcggatcg ccttgccgtg agcgcgcccg
     2401 aattctcgcg gatggtcgac ctgcgccaga tcacggcggc gccggtgcag ctgagcgcca
     2461 acgaggggga gcgcaaggcg ctcgcgggcc ggttcgggct ggtctcgctt gaccggctcg
     2521 aggcgacggt gagcctggaa gccgatggcc cggtggtttc cgccaagggc cggatcagcg
     2581 cggtctgggt ccagccctgc gccgtttcgg gcgaggacct cgcccagtcc gccgacgagg
     2641 agattgacct gcgcttcgtg cctgcgcgga ccgatcatgc gccggacgag gaaatcgaac
     2701 tgtccgaaga cgagctggac gagatcgaat ataccggcac cagcttcgac ctcggcgagg
     2761 cggtggccca gtcgctggcc ctggcgatcg atcccttcgc gtcagggccc gaggccgagg
     2821 cggcgcgcaa ggcggcgggg atcgtcaacg agggacaggc cggggccttt gcggcgctgg
     2881 cggggttgaa gctgccgaag gattgagcgg gcgcaagctg gcggggtcac cccccagcca
     2941 gctgcgacca ggcagcaagc ccgcaagcga agcctggatg ggggtgacct agcccccagg
     3001 cgcaaccgtc agaacggaat gtcgtcgtcc agatcgtcgg cgaagccgcc tgcgccgccc
     3061 gtgccgcccg cgccgccgcc gccctggttc cagccgcctg acgaaccgcc gcccgcgcgg
     3121 ccgctgctgc tgccctggcc atagccgccg ccggaggagc cgccctcgtt ccagctgcgg
     3181 ccgccgctgg cgccggcgcc gctgccgcct tcacggcccg cgccacccga tccgtcgagc
     3241 atggtaaggc tgccgcccgg gccgaccgaa atctcggtgg tgtagcgatc gttgcccgag
     3301 gcatcctgcc acttgcgggt gcgcagctgg ccttcgatgt aaactttcga gcccttgcgc
     3361 aggtaacgct cggccacgcc gaccaggcca tcgctctgca agacgaccga gtgccactcc
     3421 gtccgctcct tgcgttcgcc ggtgttgcgg tccttccagc tttccgacgt ggcgatcctg
     3481 aggttggcga tgcgcccgcc gttctggaaa gacttcactt ccgggtcttg gcccaggttc
     3541 ccgatcagga tcaccttgtt gacgctgcca gccatcgaat cacgttcctc ttgaatctgg
     3601 ccagtgggtt agcccatcgg gataccaggg tgcgagtccg cgcggcaggt ttcccccatc
     3661 acgatgagcg tggcgcctaa agccccagcg ccaccgcgct ccagtaggtc accccggccg
     3721 ccacataggc gaggccgaac aggtaggcga gcatgaaggc tggccatttc cagccgttgg
     3781 tctcgcgccg ggtgacggcg atggtgctca tgcactgggg cgcgaagacg aaccaggcga
     3841 ggaaggccag cgccatcggc agggtccact tgccgcgcag gcgatcgccc agttgctgcg
     3901 aaccggcggc atcgtcagcc gcggccacgg cataggtcgt cgccagcgag gagacggcaa
     3961 cctcgcgcgc ggccatggcc gggatcagcg agagcgccat gtcgcggttg aagccgatcg
     4021 gttcgaccac gacggcgagg ccattggcga tgtgcccggc aagcgaggca tcgacctggt
     4081 tctggccatc ctgcgcgcgg gggaagttca gcagcagcca cagcacgacg gtgaccgtga
     4141 agatgatcgt gccggcgcgg cgcaggaaga tccacgcgcg ctgccacagg ccgatcgcca
     4201 tgtccttgac gcggggcatc tggtacttcg gcagctccat gatgaagccc gaggccgcgc
     4261 ccttggtgac cgagcggcgc aggatcagcg ccacggccat ggccccgacg atccccgcga
     4321 catagagcgc cagcagcacc agcccctgca ggcccacgcc ccagccgacc gtgcgattgg
     4381 ggatgaaggc ggcgatgatc acggcataga ccggcaggcg ggccgagcag gtcatcatcg
     4441 gggcgatcag gatcgtggtc agccggtcct tgggatcggt gatcgatcgg gtcgccatga
     4501 tcccgggaat cgcacaggcg aaggaggaca gcagcggaat gaagctgcgc cccgacaggc
     4561 cgaccccggc catcatccgg tccatcagga aggccgcgcg ggccatgtag ccgctcgctt
     4621 ccatggcgag gatgaagaag aacaggatga cgatctgcgg caggaacacc acgaccgagc
     4681 cgacgcccga gatgatcccg tcggtcaaca gatcgcgcgc aaggctgggc ggcatggcgg
     4741 cgcggacctg atcgcccagc cacgagaccg cgccatcaag cgcatcggca aagggtgtgg
     4801 ccccggcgaa gaccgcctgg aacaccacga acagcagggc gaacaggatc accggtccga
     4861 tccacgggtg cagcaggagc ttgtcgagct tggcgtggag gcggtgacgg cgggtttccg
     4921 aaacgatcgc cgcctcggca atggcatggg ctgagatgcg ccgctcggta agatcgatgt
     4981 gggggcgcgg gtggccggga ttgggctcgg ccgcgcgggc ctcggcactg gcgatggcgg
     5041 cggcaagctc gtcgagcccg cgccggcgca ccgcgaccgt ggggacaacc ggtacgccga
     5101 gggcctgttc gagcgcctgg ggatcgagga ccagcccgtc gcgctcggcc aggtcgatca
     5161 tgttaagcgc gacgacggtg ggccggccca gggcgatgac ttcctgcgcg aagaccaggt
     5221 gctgctcgag gttcgaggcg tcgagcacga ccaccagcac ttcgggcgcg gcctcgccgg
     5281 ggaatcttcc catgatgacc ttggcggtca cttcctcgtc cgggctggtc ggatgcaagc
     5341 cgtaggagcc gggcaggtcg gtcagttcga ccggttcgcc cgagggcagg accagccgcc
     5401 cgctcttgcg ctcgaccgtg acgcccggat agttggcgat cttctggcgc gcgccggtga
     5461 gcgcgttgaa cagggcgctc ttgccggcat tgggattgcc gaccagcgcg gcggagcgct
     5521 gccggctcat gccgccgccc cgttcgcccc ggccgtctcg gccaccggct cgacctccat
     5581 cgccgcggcg tggacgcggc gcagggccac ggtcatccgg ccgaccatca ccgccagcgg
     5641 atcgcgcccg ccgaacacgc cgcgatgggc gagcgccacg cgcgcgccgg tatcgatgcc
     5701 gagcgcgcgc aggcgctggc cctcctcgtc caccaaccgc gaccagtcga ccgcgacgat
     5761 gcgggcgcgc ttgccgatgg ggagctggtc gagagtcatg agtcggcgat gccgcatgca
     5821 gcggctaatt gcaagttatt ctcaataagc gtcacgccgg tcccccccgc gcctgctgtc
     5881 tgcgacgcgg cgccggctct gtccatgcac gtggcggatc aacacagcct tgatcgaggt
     5941 catcaaacga ggggagaata catgcccgtt cagttcgcgg gatagcgcag ccggcccagc
     6001 agccgggtaa tgctgaagct gtcttcgcgc gggcgcagca ggcgggcctt ggccttttcg
     6061 aagcgcatga ccccggcgat ccggcgatcg aggaaggcgc gggtctcggc cttgccctcg
     6121 ctctcatcgg ccacgaaggc cagcagggtg gccccgtaga gcgcggccag ggtggcgcgc
     6181 ttggtataat ggttgaagtc cgccgccgtg tcgccggcga ggcgccacat gcggtcggcg
     6241 ctggaccatg acagtttcgc ggtgcgcgcg gcgttctgcg gcatggccat gatggcgagc
     6301 gcccggcgca gcgcttcctc gcgccccgcg acggcatcga ggcggaactg gacgaggctg
     6361 cggatgcgct cacggatcgg caggttgccg agccgctcgg cgggaagcgc cgcggccatg
     6421 tcggcatcga tccgggcgat ccaggcggcg atcatgtcca tcgccccgcc ggggaaggcg
     6481 atccgcgcga cctccggctt caccccatgg gcgagggcgg cattggtgac ggcgttcttg
     6541 ctccacccat cgaaaatggc ggcatcggcg atggccgggg ccagggcgag gcggagttcg
     6601 tcgagggtca gatcggaata gtccatcggt gctgcctcag aacgaggggc cataggactg
     6661 cttgcgcttg gcggcagcgg ccgtggccgc ggcgtcgatc gcgccccaga tcgcgctgtc
     6721 cttgccctgc aactcggcat aatcgcgcgc gttccggccc gaattgtcgg cccgcagcgg
     6781 atcggcgccg gccttgatca gcaggcggac gagttcgacg ctgtgctggt ggaccgcggc
     6841 gatcagcggg gtctcgccgg tgccatccgg atcgttgggg cgcgcgccct gatcaagcag
     6901 gaggtcggcc ccatcagccc agtccagcgc aacggcgacg gcgagcgggg tcaccccctt
     6961 gtcggtccgc aggttggggt tggcgccctt ggccagcagg aaactgagcc aggtgagatc
     7021 gcggcgctgg gtgacgatgt gcagcgccgt ctc
//
LOCUS       DATNBV010000134         3411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1353334,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000134 DATNBV010000000
VERSION     DATNBV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353334"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VN627_03220"
     CDS             <1..936
                     /locus_tag="VN627_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02297.1"
                     /translation="LQAAGVKPAAMVNNVPQEPMEGISFAATFTAPGSPRDGRPQYSE
                     MFGNRSMAAQGWSIVANHRMATWRFDAPPTFNEPWELYDLVKDPGQTTDLAAKMPGKV
                     AELARLFDAQAARYHVLPSHNQSEGMPEMAKLSAAQYAARGGVWRYSGPVSSVPGLLA
                     PPILSRSFRMTARLDVPAAATTGPIFAQGSTLGGMALYLDQGRPVLLLSQIDGAAARI
                     AAPAELPDGTTELTLDVDRGPRGADGRSAFTVRIGAAGKVLAEQVVQMQVDPAVPLAE
                     TFAIGRDDAAPMLAGYPASKPLAAGLSDVRFDFNR"
     gene            978..>3411
                     /locus_tag="VN627_03225"
     CDS             978..>3411
                     /locus_tag="VN627_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXD02298.1"
                     /translation="MAMRRLAVPLVLLAVAGCAHQTSQRLVDPGTPRAEAPAAEPQPA
                     PLSALVDAVAIRYEKFTLANGLTVLIHTDRKAPIVGVTTYYRVGSKNEPRGKTGFAHL
                     YEHLFFGGSENVPNFDIPLEGAGSTATNGSTWYDRTNYVETVPTGALDRALMMEADRM
                     GHLLGAVTQDKLDKQRGVVQNEKRQGDNQPYGLMEYAIGEGLFPIGHPYRHSTIGSMA
                     DLDAATLRDVRAWFHDHYGPNNVVLALAGDIDAATARPLVEKWYGDIPAGPPVTPVAA
                     PPVTLPAPARRDMADQVPTLRLTRNWSGPGLNDADAPALMVGMDILGGLASSRLDNAL
                     VRGQQLAVAVTASAQQFEEISQLQASMDVKPGVDRARAEAAFDAVIARLVGEGPTADE
                     VRRSATRLASAQIGALEQVGGFSGKGATLAEGQLYSGDPEKFRRDLQAIAALTPAQVQ
                     AALAKWLGRPVFALAVVPGPRTERGDQMGGWGDEGASPPPPKDPKQPVPAIRQGPKRA
                     IPPVAPVGELAFPALERATLSNGIAVTLARRTAVPKVMVSLSFDAGTAADRLDTPGTQ
                     GTMLALLDEGTATRNATQIAEDQERLGATISLQAGLDTSMVTLDALTTNLAPSLALMA
                     DLVRNPAFRPEDVARVKDQRLAELAQTEASPGALAYRTMAPLLFGPAHPYGLPGDGYG
                     TAPALSALTPEALRAAHQRWLRPDLARITVVGDITMARLVPLLEQAFGGWTAPVAPRP
                     AKPMAAAIPAPAPRILVVDRPNSPQSYILAGRVLPLTGRDQGREALDLANEVLGNGFL
                     SRLNLDLREDKGW"
BASE COUNT          518 a         1291 c         1122 g          480 t
ORIGIN      
        1 ctccaggccg cgggcgtgaa accggcggca atggtcaaca acgtaccgca ggagccgatg
       61 gaagggatca gcttcgccgc caccttcacc gcgccgggca gtccgcgcga cggtcggccg
      121 cagtattcgg aaatgttcgg caaccggtcg atggccgcgc aaggctggtc catcgtcgcc
      181 aatcaccgga tggccacgtg gcgcttcgat gccccgccca cgttcaacga accgtgggag
      241 ttgtacgatc tggtcaagga tcccgggcag acgaccgatc tcgccgcgaa aatgccgggc
      301 aaggtggccg aacttgcgcg cctgttcgat gcgcaggccg cccgctacca tgtgcttccc
      361 tcgcacaacc agagcgaagg catgccggag atggccaagc tttccgctgc ccagtatgcc
      421 gcgcgcggcg gcgtgtggcg ctattccggc ccggtcagca gcgtacccgg cctgctggca
      481 ccgccaatcc tcagccgcag cttccgcatg accgcgcggc tggacgtgcc cgcggcggca
      541 accacggggc cgatcttcgc ccaaggcagc acactgggcg gcatggcgct gtacctcgat
      601 cagggccggc cggtcctgct gctcagccag atcgatggcg ccgctgcccg catcgccgcg
      661 ccggccgaac tgccggacgg caccaccgag ctcacgctcg atgtcgaccg cggcccgcgc
      721 ggcgcggacg gccgcagcgc cttcaccgtg cggatcggcg ctgcgggcaa ggtgctggcg
      781 gagcaggtcg tgcagatgca ggtcgatccg gccgtgccgc ttgccgaaac cttcgccatc
      841 gggcgcgacg atgccgcgcc gatgctggcg ggctacccgg ccagcaagcc gctcgcggcg
      901 gggctgtcgg acgtccggtt cgatttcaac cgctagggtt aggaggcgct ggcgcggcga
      961 ggaaatctcg ctagaccatg gccatgcgcc gccttgccgt cccgctcgtc ctgctggctg
     1021 tcgccggctg tgcccaccag acctcgcagc gccttgtcga tcccggcacg ccgcgggccg
     1081 aggcgcctgc cgccgagccc cagcccgctc cgctctccgc cctcgtcgat gccgtggcca
     1141 tccgctacga gaagttcacg ctggcaaacg gcctgaccgt cctcatccat accgatcgca
     1201 aggcgcccat cgtcggggtc acgacctact accgtgtcgg ctccaagaac gagccgcgcg
     1261 gcaagacggg ctttgcccac ctctacgagc acctgttctt cggcggctcg gaaaacgtgc
     1321 ctaacttcga cattccgctc gaaggtgccg gctccaccgc gaccaacggc tcgacctggt
     1381 acgatcgcac caactacgtc gagaccgtcc ccaccggcgc gctcgaccgg gcgctgatga
     1441 tggaggcaga ccggatgggc cacctcctcg gcgcggtcac gcaggacaag ctggataagc
     1501 agcgcggcgt cgtccagaac gagaagcggc agggcgataa ccagccctac ggcctgatgg
     1561 aatatgcgat cggcgagggg ctgttcccca tcggccatcc ctatcgccat tcgaccatcg
     1621 gctcgatggc cgatctcgat gccgccacgc tgcgcgacgt gcgcgcctgg ttccacgatc
     1681 actacgggcc gaacaacgtg gtgctcgcgc tggccggcga tatcgacgcg gccacggccc
     1741 gcccgctggt ggaaaagtgg tacggcgaca ttcccgccgg gcctccggtc accccggttg
     1801 ccgcgccgcc ggtcaccctg ccggccccgg ccaggcgcga catggccgat caggtgccga
     1861 ccttgcggct cacgcgcaac tggagcggcc cgggcctcaa cgatgccgat gccccggccc
     1921 tgatggtcgg catggatatc ctcggcggcc tcgcctcctc gcggctcgac aatgcactgg
     1981 tgcgcgggca gcagctggct gtggccgtta cggcctcggc ccagcagttc gaggaaatca
     2041 gccagttgca ggcgagcatg gacgtcaagc ccggcgtgga ccgggccagg gcggaagcgg
     2101 ccttcgacgc ggtcatcgcc aggctcgtcg gcgaaggccc caccgccgac gaggtccgcc
     2161 gctccgccac gcgccttgcc agcgcccaga tcggcgccct ggagcaggtt ggcggctttt
     2221 ccggcaaggg cgcgacgctc gccgaagggc agctctattc cggcgatccc gagaagttcc
     2281 gccgcgatct ccaggccatc gccgcgctga ccccggcgca ggttcaggct gcgctggcga
     2341 agtggctggg ccgcccggtc ttcgccctcg ctgtcgtccc cggcccgcgc accgagcgcg
     2401 gggaccagat gggcggctgg ggtgacgagg gtgcaagccc gccgccgcca aaggacccga
     2461 agcagccggt gcccgccatc aggcaggggc caaagcgcgc catcccgccg gtcgctccgg
     2521 tgggagaact ggccttcccg gcgctcgaac gggcgaccct ctcgaacggg atcgcggtca
     2581 ccctcgcccg ccgcaccgcc gtgcccaagg tcatggtcag cctgagcttc gatgccggca
     2641 ccgccgccga ccggctcgat acgccaggca cccagggcac catgctcgcc ctgctggacg
     2701 aaggcaccgc gacccgcaac gccacccaga tcgccgagga ccaggagcgg ctcggcgcca
     2761 cgatcagcct ccaggccggg ctcgacacca gcatggtcac gctcgatgcg ctgaccacca
     2821 acctcgcccc ctcgctggcg ctgatggccg acctcgtccg caatcccgct ttccggcctg
     2881 aggacgtcgc ccgggtcaag gaccagcgcc tcgccgaact ggcgcagacc gaggccagcc
     2941 ccggggcgct cgcctatcgc accatggcgc cgctgctgtt cgggccggcc catccctatg
     3001 ggctgcccgg cgatggttat ggcacggcac cggcgctgag cgcactcacc cccgaagccc
     3061 tgcgcgcggc gcaccagcgc tggctgcggc cggatctcgc ccggatcacc gtggtcggcg
     3121 acatcaccat ggcccggctc gtccccctgc tcgaacaggc attcggcggc tggaccgccc
     3181 ccgtcgcgcc gcgcccggcc aagccgatgg ccgcggccat ccccgcgccc gcgccccgca
     3241 tcctcgtggt ggatcgcccg aattcgccgc aaagctatat cctcgccggc cgcgtcctgc
     3301 cgctgaccgg gcgggatcag ggccgcgagg cgctggacct cgccaacgag gtgctcggca
     3361 acggcttcct ctcgcgcctc aaccttgatc tgcgcgagga caagggctgg t
//
LOCUS       DATNBV010000135         9427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2947454,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000135 DATNBV010000000
VERSION     DATNBV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2947454"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..725
                     /gene="rpsF"
                     /locus_tag="VN627_03230"
     CDS             363..725
                     /gene="rpsF"
                     /locus_tag="VN627_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXD02299.1"
                     /translation="MALYEHVFLARQDLSQAQVDALAATATEIVEANGGKVTKTETWG
                     LKGLAYKIKRNRKAHFVLLNVEAPAGVVAELERQTQINEDILRYMTVRVDELEAGPSV
                     QMRKNDRERTRRRDREEA"
     gene            729..953
                     /gene="rpsR"
                     /locus_tag="VN627_03235"
     CDS             729..953
                     /gene="rpsR"
                     /locus_tag="VN627_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXD02300.1"
                     /translation="MARAFFRRRKSCPFSGKNAPKIDYKDVRLLQGFMSERGKIVPSR
                     ITAVSAKKQRELSQAIKRARHIGLLPYLVK"
     gene            967..1568
                     /gene="rplI"
                     /locus_tag="VN627_03240"
                     /pseudo
     CDS             967..1568
                     /gene="rplI"
                     /locus_tag="VN627_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
     gene            complement(1632..3938)
                     /locus_tag="VN627_03245"
     CDS             complement(1632..3938)
                     /locus_tag="VN627_03245"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXD02301.1"
                     /translation="MRADRAIRSGLTVGMAALLLASAAPALPAPPAAVTGFPAGPTAP
                     AGAPNVLIVMTDDVGFAAPSTFGGGIPTPNLDALAAGGLRYVNFHTTALCSPSRAALL
                     TGRNHHAVGAGHVPELATGEPGYTSVLPPSAATFARVLGRSGYASAFLGKHHNVPSWE
                     DGPSGPFDRWATGLGFDYFYGFHGGMTNQFSPDLIENRTPVETPREADYFLDRDLADH
                     AIDWLATHRASHQGRPFLLYLAPGTAHAPLQAPAEWLARFRGKFDAGWDAYRAETFAR
                     QKAQGLIPANAVLPPLPPGIAPWATLSAKQKAVYARYMEAYAAALACFDDQFGRVIAT
                     LRQSGELDNTLVLFISGDNGATPEGGANGAMNYLGRTAPDKELDWAYDHLDQIGGPNS
                     YPVGSAGWAVALNTPWPYYKVVASRLGGITNGMVVSWPARIKTPGIRHQWLDLTDIAP
                     TLYQAIGVTAPAVVDGVRQMPLDGASFLATLADPDAPSPHPRQYFEINGNAAIYDRGW
                     LLATRMQDSTAAGNTMPDLAAPWQLYDLANDPTQTVDVAGRYPARAKALRALFDAEAR
                     RNHVLPLQTTSGARLSSAVRPEPGNAAGRYIYRPGRWVFGKGTFPSLLGRDWTISADV
                     TVPADGLASGTLVTQGGQFQGWGLLVHGGIPAFVYRTGDMPAQITRVARHEALSPGAH
                     RISVSLARDAAGQGGELRLTVDGQPAEAAHVPQVGAFMLGNEPVAIGRDTGTTVDGDP
                     LPFAWRGDLTVTLDMGPIAGPPALLPGH"
     gene            4043..5470
                     /locus_tag="VN627_03250"
     CDS             4043..5470
                     /locus_tag="VN627_03250"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HXD02302.1"
                     /translation="MKQAHAVVAELTSLYDEAIATLRADIAAFVAHGTAPPPERRANR
                     AWCYPELRLHYAGKETRPDLARAFGRLSHPGTYACTVTRPALFADYLLEQLALLGDDY
                     AITLEVARSDQEIPFPYVIDASGGLGAVTPLDLARHFPTTELAMIGDELADGADPQPG
                     APLPLALFDALRTDFSLARLAHYTGTAPEHFQRFILFTNYHRYVDEFVDWAAGQLGER
                     YTALSGAGGLYVDAPLANARARLSDTAWRRHQMPAYHLIGPDRSGISLVNIGVGPSNA
                     KTICDHLAVLRPEAWLMIGHCGGLRDTQKIGDYVLAHAYLRDDHVLDDVLPPEIPVPA
                     IAEVQVALARAAELVSGDSGAGLKKRMRTGTVVTTDDRNWELRFTHSARRLSLSRAVG
                     IDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEEAIAAHLKIGTLA
                     CELLRDEGERLHSRKLRAFNEPPFR"
     gene            complement(5598..6365)
                     /locus_tag="VN627_03255"
     CDS             complement(5598..6365)
                     /locus_tag="VN627_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXD02303.1"
                     /translation="MRVATEFVPGFDGQPLAVHRLGEGRPLLLLHGLFSNAEVNWVKY
                     GTAQALAEAGFACIMPDLRAHGQSAHPHDPAGYPHDVLVRDLVALVGELGLADYDLGG
                     FSLGARTALRGVIAAGLAPRRLVLGGMGLAGIRGWTGRIEFFLDVITRFGTIPREDPA
                     YMAQNFMRSTRVDLVAARLLLESFIAGAAEAESENLGLATMPSLLVCGTEDDDNGSAE
                     ELAAALSDGRYAPIPGNHMSCVTRPELATAIGDFLLA"
     gene            complement(6376..7155)
                     /locus_tag="VN627_03260"
     CDS             complement(6376..7155)
                     /locus_tag="VN627_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXD02304.1"
                     /translation="MLEHFDWLHGVAGLFVGTLVGITGVGGGSLMTPILVLLFGVSPQ
                     TAVGTDLLFAALTKTAGTATHGWRATIEWRVVRRLASGSVPAAILTLIVLSQVGKFGK
                     NTSDVIVTVLGWMLIFTSIATLLQKRLAVFAKRQERLDEDESLWPTVVLGAVIGVAVT
                     VSSVGAGAIGVTALLMLYPALRVSRIVGSDIAHAVPLTLVAGFGHWLIGGVDFMLLGN
                     LLIGSIPGVVLGSMLSSRAPDKLLRPALAAVLLFSGFKLLS"
     gene            7352..8023
                     /locus_tag="VN627_03265"
     CDS             7352..8023
                     /locus_tag="VN627_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02305.1"
                     /translation="MYRTRTLTGLLMAGALLAGGASPAFASKADRAAQAIAAAQAKID
                     SAKTIGAGTEVPRRVAAAQAELARASEAKQSGHNEDAIQIAIHAQAMADAILGETTAR
                     KDADVAATRAAAQDQTAAAHSQAEAARAEAAAANARAEAAQQAAQQSAQQAAAATQAA
                     VAAAQTPPPAPEPTKVETTLTTATPATGAHRTVKKKVVVHRKVRGSTHAATVTTATTK
                     VSTGQ"
     gene            complement(8127..9152)
                     /locus_tag="VN627_03270"
     CDS             complement(8127..9152)
                     /locus_tag="VN627_03270"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXD02306.1"
                     /translation="MGYRVVVVGATGNVGREMLNILAERSFPIDEIAAVASPRSTGTE
                     IEFGETGQMLKVKNIEHFDFTGWDIALFAAGSGPTKTYAPKAASQGCVVIDNSSLYRM
                     DPDVPLIVPEVNPEAIDGYKARNIIANPNCSTAQMVVALKPLHDKAKITRVVVATYQS
                     VSGAGKEGMDELFEQSRAIFVGDQVEPKKFTKQIAFNVIPHIDVFLDDGYTKEEWKMV
                     AETKKILDPKIKLTATCVRVPVFVGHSEALNIEFENEISATEAQDILREAPGVMLVDK
                     REDGGYVTPVECVGDYATYVSRVREDITVENGLALWCVSDNLRKGAALNAVQIAELLG
                     RRHLKKG"
     gene            complement(9276..>9427)
                     /locus_tag="VN627_03275"
     CDS             complement(9276..>9427)
                     /locus_tag="VN627_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXD02307.1"
                     /translation="RVFPLYSPNIDSITVVRKGVVRRAKLYYLRGRTGKRARIAERRD
                     TRQEG"
BASE COUNT         1692 a         3236 c         3079 g         1420 t
ORIGIN      
        1 gcggcccgca cggccagcgt tccgcccgag cgcggggcga ccgtgctcac cgcatctgcg
       61 aaggcatcag gctcagcgca aacttctgag gtcatgatct ttactcctga gaggcggctg
      121 ataattcgca catgcagcag aagagtcatt gacctgccgc aaggagcggc tgcgggcatc
      181 gcgaaatccg gagaacgcgg cgccaatcat gcaaacggca gcttgcattt ccacccgcct
      241 gcgttaaggg ccgccacttg cacgcttccg atccggattc gcgcaaacct gaagaaagcc
      301 ggaggggccc cgcaatcccg cggatcagcc agcgatcggt tgaaagattg aaaggaaagg
      361 ccgtggctct ttacgagcac gtgttcctgg cgcgccagga cctcagccag gcgcaggtcg
      421 atgcgctggc tgctaccgcc accgaaatcg tcgaagcgaa cggcggcaag gtcaccaaga
      481 ccgaaacctg gggcctcaag ggcctcgcct acaagatcaa gcgcaaccgc aaggcgcact
      541 tcgtgctgct gaacgtggaa gcccccgccg gcgtcgtcgc cgagctggag cgccagacgc
      601 agatcaacga agacatcctg cgctacatga ccgtgcgcgt ggacgagctc gaagccggcc
      661 cgtcggtcca gatgcgcaag aacgaccgcg agcgcacccg tcgccgcgac cgcgaggagg
      721 cataagccat ggcccgtgcg tttttccgcc gccgcaagtc ctgcccgttc tcgggcaaga
      781 acgcgcccaa gatcgactac aaggacgtgc gtctcctgca gggcttcatg tccgagcgtg
      841 gcaagatcgt cccgagccgc atcaccgcgg tttcggccaa gaagcagcgt gagctgagcc
      901 aggcgatcaa gcgcgcccgg cacatcggcc tcctgcccta cctcgtgaag taaggaggga
      961 ctccccatgc agatcatcct cctcgaacgc atcgagaagc tcggcaccat cggcgacgtc
     1021 gtgaacgtca aggacggcta cgcccgcaac ttcctgctcc caacaagaag gccctgcgcg
     1081 ccaacgacgc gaaccggaag gtcttcgaag ccaaccgcgc ccgcatcgag gccgacaacg
     1141 ccgagcgcaa ggcgcacgcc gaagtccact cgaaggatgt cgaaggccag caggtcatcc
     1201 tcatccgcgc ggcctcgaac gccggccagc tttacggctc ggtctcggtg cgtgacatcg
     1261 tcgatgccct gaacgccgaa ggcgccaagg tgtcgaaggc gatgatcgtg ctcgagcgcc
     1321 cgatcaagac catcggcgtg ttcgacgtgc gcgtcgcgct gcacccggaa gttgccgtca
     1381 acgtcaaggt gaacgtcgcc cgttcgcccg acgaggccga actccaggcc cagggcgtca
     1441 acgtcatcga cgcgatgttc gagaccgaga cctcgggctt caccgaggcc tacgatccga
     1501 acgccgaacc cggcgagatc ccggccgaac tgctcgacga gcaggccgag ggcaccgccg
     1561 aggcgtaaca accgtttccc gcaagggatt ggaaacccct cgctccgacg ggagcggggg
     1621 gtttttccgt ttcagtggcc cggcagcaga gccggtgggc ccgcgatagg ccccatgtcc
     1681 agcgtcacgg tcagatcgcc ccgccaggca aacggcaagg gatcgccgtc gacggtggtc
     1741 ccggtgtcgc gcccgatagc caccggctcg ttgcccaaca tgaaggcgcc gacctgcggc
     1801 acgtgggcgg cctcggctgg ctgaccatcg acggtcagcc gcagttcgcc cccctgccct
     1861 gccgcatcgc gcgccaggct gaccgaaatg cggtgcgccc ccggcgagag ggcctcgtgc
     1921 ctggccacgc gggtgatctg ggccggcata tcgccggtgc gatagacgaa tgcgggaatt
     1981 ccgccgtgca ccaggaggcc ccatccctgg aactgccccc cttgggtgac cagggtgccg
     2041 gaagcgagtc catcggccgg caccgtcacg tcagccgaaa tcgtccagtc gcggcccagc
     2101 aacgacggaa aagtgccctt gccgaagacc cagcgccccg gccggtagat atagcggccc
     2161 gccgcattgc cgggctctgg ccgaaccgcc gaggacaggc gcgcgccgct ggtggtttgc
     2221 agcggcagca catggttgcg ccgggcctcg gcatcgaaca gggcccgcag cgccttggcc
     2281 ctcgccggat agcgccccgc aacgtcgaca gtctgggtcg gatcattggc aaggtcatac
     2341 agctgccatg gcgcggcgag gtcaggcatc gtatttccgg ccgccgtcga atcctgcatg
     2401 cgggtcgcca gcagccatcc ccgatcatag atcgcggcat tgccgttgat ctcgaaatac
     2461 tgccgcggat gcgggctggg agcatcgggg tcagcaagcg tggcgaggaa acttgcgccg
     2521 tccagcggca tctgccgcac accgtcgacc accgcaggcg ccgtgacgcc gatggcttgg
     2581 tagagggtgg gcgcgatgtc ggtgaggtcg agccactgat ggcggatgcc tggggtcttg
     2641 atccgggcgg gccagctcac caccatgccg ttggtgatgc cgccgagccg cgaggcaacg
     2701 accttgtagt acggccaggg cgtattgagc gcgacggccc agccggccga ccccaccggg
     2761 taggagttgg ggccgccgat ctggtcgaga tggtcatagg cccagtccag ttccttgtcg
     2821 ggtgccgtcc gcccaagata gttcatcgcc ccgttggctc cgccctccgg ggttgcgccg
     2881 ttgtcgcccg agatgaacag caccagcgtg ttgtccagct cgccgctctg cctcagcgtg
     2941 gcgatcaccc ggccgaactg gtcatcgaaa cacgccagcg ccgccgcata ggcctccata
     3001 tagcgcgcat agacagcctt ctgcttggcc gagagcgtcg cccaaggcgc gatcccgggc
     3061 ggcaggggcg gcagcaccgc gttagccgga atgagcccct gagccttctg acgggcgaag
     3121 gtttccgctc gataggcatc ccagccggca tcgaacttgc cccggaaccg ggccagccat
     3181 tcggccggtg cctgaagtgg cgcatgggcg gttcccgggg cgagatagag caggaacggc
     3241 cggccctgat gactggcgcg atgggtggcc agccagtcga tcgcgtggtc ggccagatcc
     3301 cggtcaagga aatagtccgc ctcgcgcggg gtttcgaccg gggtgcggtt ctcgatgagg
     3361 tcgggcgaga actggttcgt catcccgccg tggaagccgt agaaatagtc gaaccccaga
     3421 ccggtggccc agcgatcgaa cggtcctgac ggaccatcct cccagctagg gacattgtgg
     3481 tgcttgccaa ggaaggcgct ggcatagccg ctgcgcccga gcacccgggc gaaggtcgcc
     3541 gcgctgggcg gcaggaccga ggtgtagccc ggttcacccg tggccagctc gggcacatgc
     3601 ccggcgccca cggcgtggtg attgcgcccg gtcagcagtg ccgcgcggga gggtgagcac
     3661 aaggccgtgg tgtggaaatt gacataacgc agcccgccgg cggcgagcgc atcgaggttg
     3721 ggcgtcggaa tgcctccgcc gaaagtgctg ggggcggcaa atcccacatc gtcggtcatg
     3781 acgatcagca cattgggcgc gccggcagga gcggtcggac ctgcgggaaa gcccgtcacc
     3841 gccgcgggcg gggcgggcaa ggctggcgcc gccgaggcca gcaacagggc cgccatgccc
     3901 accgtcagtc cgctccggat cgctcggtcg gccctcatcg caaattctcc catggcttgt
     3961 gcagagggta tgccgcacca agatcatcgg caagctgctg ttgcatctgg aaaccgccgc
     4021 ccgaaccgct acccttgcag gcatgaagca agcccatgcc gtcgtcgccg agctcaccag
     4081 cctctacgac gaggccatcg ccaccctgcg cgccgacatc gccgcgttcg tcgcccacgg
     4141 caccgcaccc ccgccggaac gccgcgccaa ccgggcctgg tgctaccccg aactccggct
     4201 gcactacgcc ggcaaggaga cacggcccga cctcgcccgc gctttcggcc gcttgtcgca
     4261 cccgggcacc tatgcctgca cggtgacccg cccggcgctg tttgccgact atctgctgga
     4321 acagctggcc ctgcttggcg acgattatgc gatcaccctc gaggttgccc gttcggacca
     4381 ggaaatcccc ttcccttacg tgatcgacgc cagcggcggg ctcggtgccg tgaccccgct
     4441 cgatcttgcc cgtcacttcc cgacgaccga attggccatg attggcgacg aactggccga
     4501 cggtgccgat ccccaacccg gcgccccgct accgcttgcc ctgttcgatg cgctgcgcac
     4561 cgatttcagc ctcgcgcgcc tcgcccacta taccggaacg gcgcccgagc acttccagcg
     4621 cttcatcctg ttcaccaatt atcaccgcta cgtcgacgag tttgtcgact gggccgccgg
     4681 gcagttgggc gagcgctata ccgcgctgtc cggcgcgggc gggctctatg tggatgcccc
     4741 gctggccaat gcccgcgcgc ggctgtcgga cacggcctgg cggcgccacc agatgccggc
     4801 ttaccacctg atcggcccgg accgcagcgg catcagcctc gtcaacatcg gcgtaggccc
     4861 gtccaacgcc aagaccatct gcgatcatct tgcggtcctc aggccggagg cctggctgat
     4921 gatcggccac tgcgggggct tgcgcgacac gcagaagatc ggggactatg tgctcgccca
     4981 tgcctatctg cgcgacgatc atgtgctcga cgacgtgctc ccccccgaaa tcccggtgcc
     5041 cgccatcgcc gaggtccagg tagccctcgc ccgcgcggcg gaactggtca gtggcgacag
     5101 cggcgcgggc ctgaagaagc gcatgcgcac cgggacagtc gtcaccaccg atgaccgcaa
     5161 ctgggaactg cgcttcaccc attcggcgcg gcgcctctcg ctctcgcggg cggtggggat
     5221 cgacatggaa agcgccacca tcgccgcgca gggataccgc tttcgggtgc cctacgggac
     5281 actgctgtgc gtctccgaca agccacttca tggcgaaatc aagctgcccg ggcaggccaa
     5341 ccggttttac gaggaagcca tcgccgccca cctgaagatt ggcaccctcg cctgcgaact
     5401 cctgcgcgac gagggcgagc gcctgcattc acgcaagctg cgcgccttca acgaaccgcc
     5461 gtttcgctga ggccggtagc cgaggcgaac ggatcagcga aggtcgacac ccggaagctc
     5521 gatccaagca gcaggcgatg ctcgcgctgg gccaaaatca gctccgtccc ggcgcatcaa
     5581 cctacgatga tcatgctcta ggccagcagg aaatcgccga tcgcggtggc cagctcgggc
     5641 cgggtgacgc agctcatgtg attgcccggg atcggcgcgt accggccatc cgacagggct
     5701 gccgcgagct cttcggcgga gccgttgtca tcgtcctccg tcccgcagac cagcaggctc
     5761 ggcatggtgg caaggcctag gttctcgctc tcggcctctg ccgcgccggc gatgaagctt
     5821 tccagcagta ggcgggctgc gacaagatcg accctggtcg agcgcatgaa gttctgggcc
     5881 atgtaggccg ggtcctcgcg cggtatcgtg ccgaagcggg tgatcacgtc gaggaagaat
     5941 tcgatccgcc ccgtccagcc gcgaataccg gccagaccca tgccgcccag caccaggcgg
     6001 cggggcgcga ggccggcggc gatcacgccg cgcagcgccg tgcgcgcgcc gagcgagaag
     6061 cccccgagat cgtagtcggc aagacccagt tcgccgacga gggccacgag atcgcgcacc
     6121 agcacatcat gcgggtaacc ggcgggatcg tgtgggtggg cgctctgccc gtgggcgcgc
     6181 aggtcgggca tgatgcaggc aaaccctgcc tcggcgaggg cctgcgccgt gccgtacttg
     6241 acccagttga cctcggcatt ggaaaacagg ccgtggagca gcagcagggg ccggccttcg
     6301 ccaagccggt gcaccgccag gggctggcca tcgaagccag gcacgaattc ggtagcaaca
     6361 cgcacgcggg acgcctcagc tcagcagctt gaacccggag aacagtagca cggcggcgag
     6421 cgccgggcgc agcagtttgt cgggggcgcg ggaggacagc atgctgccga ggaccacgcc
     6481 ggggatcgag ccgatcagca ggttgccaag cagcatgaaa tccaccccgc cgatcaacca
     6541 atggccgaac ccagccacca gcgtcagcgg cacggcatgg gcgatatcgc tgcccacgat
     6601 gcgcgagacg cgcaaagccg ggtaaagcat cagcagggca gtcacgccaa tggcgcccgc
     6661 gccaaccgag gataccgtga cggccacgcc gatcaccgcg cccagcacca ccgtgggcca
     6721 aagggactcg tcctcgtcaa gccgctcctg gcgcttggcg aagacggcaa gccgcttctg
     6781 caacagggtg gcgatcgagg tgaagatcag catccagccg agcaccgtca cgatcacgtc
     6841 gctggtgttc ttgccgaact tgcccacctg actgagcacg atcagcgtca ggatcgcggc
     6901 gggcacgctg ccagaggcca ggcggcgcac tactctccat tcgatggttg cgcgccagcc
     6961 gtgagtggcg gttccggcgg tcttggtgag ggcggcgaac aagaggtcgg tgccgaccgc
     7021 cgtttgcgga ctgacgccga acaacaggac aaggatgggc gtcatcagcg aacccccgcc
     7081 gacgccggta atcccgacca gggtccccac gaacagcccg gccacgccgt gcaaccagtc
     7141 aaaatgttca agcatggcca attcccccgc ggcgcgccgt cgcgtcccgt tgggcgtcgg
     7201 ctaaccggca ggggcggctt accgcaacaa agaatctcta tcaatgcagt tgggatttgc
     7261 atggattgcg acgaacggag tgatccggaa cgcccgggac gctccgttcg ttcgcttcca
     7321 ttgtccagga aacagcattc aggagtatga catgtatcgc acgcgcacgc tgacaggcct
     7381 gctgatggcc ggcgccctgc ttgccggtgg ggcctccccg gccttcgcca gcaaagccga
     7441 tcgcgccgcc caggccattg ctgcggccca ggccaagatc gacagcgcca agaccatcgg
     7501 cgccggaacc gaggtgccgc gtcgagtcgc cgcggcccag gcggagctcg cccgcgccag
     7561 tgaggccaag cagagcggcc acaacgagga cgcgatccag atcgcgatcc acgcccaggc
     7621 catggccgat gccatcctgg gagaaaccac ggcgcgcaag gatgccgacg tcgctgcgac
     7681 ccgggccgcc gcgcaggacc agaccgccgc cgcccacagc caggccgagg ccgcccgcgc
     7741 ggaggccgcc gcagccaatg cccgggcaga ggcggcgcag caggccgcgc agcaatcggc
     7801 ccagcaggcc gctgccgcca cgcaggcagc agtggccgcc gcgcagaccc cgccgcccgc
     7861 acccgagccg accaaggtcg agacaaccct gacgaccgcc accccggcca ccggcgcgca
     7921 tcgcacggtg aagaagaagg tcgtggtcca ccgcaaggtg cggggaagca cccatgctgc
     7981 gacggtgacc accgcaacga cgaaggtttc gaccggccag tgatgctaaa gggcccggct
     8041 gctggtgcag cccggcccta gttttaacca gttcctcccg agcagcctgc tcgggaggac
     8101 ggtggttatt gagcgaacgg gcgccctcag cccttcttca ggtgccgacg cccgagcagc
     8161 tccgcgatct gcaccgcgtt cagcgcggca cccttgcgca agttgtccga aacgcaccaa
     8221 agggcgaggc cgttctcgac cgtgatgtcc tcgcgcacgc ggctcacgta ggtcgcatag
     8281 tcgcccacgc attcgaccgg ggtgacgtac ccgccgtcct cccgcttgtc gacgagcatg
     8341 acgcccggcg cctcgcgcag gatgtcctgg gcctcggtgg cgctgatctc attctcgaac
     8401 tcgatgttca gcgcttccga gtgaccgacg aagaccggca cgcgcacgca ggtcgcggtc
     8461 agcttgatct tcggatcgag gatcttcttg gtctcggcga ccatcttcca ctcttccttg
     8521 gtgtagccgt catccaggaa gacatcgatg tgcgggatca cgttgaaggc gatctgcttg
     8581 gtgaacttct tgggttcaac ctgatcgccc acgaagatcg cgcggctctg ctcgaacagc
     8641 tcatccatgc cttccttgcc cgcgccggaa accgactggt aggtggcgac gaccacgcgg
     8701 gtgatcttgg ccttgtcgtg caggggcttg agcgcgacga ccatctgcgc cgtcgagcag
     8761 ttggggttgg cgatgatgtt gcgggccttg tagccgtcga tcgcctcggg gttcacttcc
     8821 ggcacgatca gcggcacgtc cgggtccatg cggtagagcg acgagttgtc gatcaccacg
     8881 cagccctgcg aagcggcctt gggagcatag gtcttggtcg ggcccgagcc ggcggcgaac
     8941 agggcgatgt cccagccggt gaaatcgaag tgctcgatgt tcttgacctt gagcatctgc
     9001 ccggtctcgc cgaactcgat ctcggtgccg gtcgaacgcg gcgaggcgac ggcggcgatc
     9061 tcgtcgatcg ggaagctgcg ctcggccagg atgttgagca tctcgcggcc gacgttcccc
     9121 gtcgcaccca cgacaactac gcggtaaccc atggtatcac tcctgtgcgc cgctgcggcg
     9181 acgccgaatc atgcaaggtc gaaaaagcaa aagggcgccc cggcttcgtg ccagggcgcc
     9241 ctttacggtg tttggtccgt agccggcggt gggcctcagc cctcctggcg ggtgtcgcgg
     9301 cgttccgcaa tacgcgcgcg tttgccggtg cggccgcgca ggtagtagag cttggcccga
     9361 cgcaccacgc ccttgcggac gacggtaatg ctgtcgatgt tcggcgagta gagcgggaac
     9421 acgcgct
//
LOCUS       DATNBV010000136         3957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3912604,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000136 DATNBV010000000
VERSION     DATNBV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3912604"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VN627_03280"
     CDS             complement(<1..331)
                     /locus_tag="VN627_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD02308.1"
                     /translation="MGFLEDMAGLAGKVAVVTGGAGGLGWPIARDFARAGMHVAVCDR
                     DAAAIEAVRAELSGFPVKTLLVHADVREPEAMEAFFAEIDRALGRVDVLVDVPGGGFV
                     APLMNTTP"
     gene            complement(389..478)
                     /locus_tag="VN627_03285"
     tRNA            complement(389..478)
                     /locus_tag="VN627_03285"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(442..444),aa:Ser,seq:tga)
     gene            664..1704
                     /locus_tag="VN627_03290"
     CDS             664..1704
                     /locus_tag="VN627_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HXD02309.1"
                     /translation="MLAVSPSQGQVDAAGIATSGPAAPTSAESAEEAGFRGYLQLLAA
                     RARGEGVRESTIAAVTSGLTFNSLVIALDRSQPGSSANPSAAPAFAPYRRMHVDAARI
                     AGGRQMRANVAGIAPAIEAKYGVPVQILLAIWGHETNYGRYTGDFDLARSLATLAYEG
                     RRRELFADEFVALLKMADRGVPLDRLKGSWAGAFGNPQFLPSMYLRLAVDGDGDGDRD
                     IWHSRADTLASIANYFRDAGWRAGQPWGVPALVPAGFDRGVVTSRLTAPDCPRVHARH
                     ARWLTVAEWRARGVQTLAPIADDVMATLIEPDGPGQTAYLLTGNYRVILEYNCSNYYA
                     LSVGLLADEIAR"
     gene            1688..2680
                     /locus_tag="VN627_03295"
     CDS             1688..2680
                     /locus_tag="VN627_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXD02310.1"
                     /translation="MRLPVSRNHGHPRHFGLLLLATVMSLAGLLAAPLLARQPAAPAS
                     APTGPAGDYPMVLGAPYVIDGATYTPADIMNVDQVGYAVIAPDAGPGVTIAHRTLPLP
                     SYVEVTSLKTGRTILARVERRGPMSGPDLIALSSGAAAQLGADGRLPVRVRRVNPPET
                     ERALLRTGQTAPVRMDTPMSLVNVLLRKLDPALVTPPVLPAPPAPPAPEPATTDVARK
                     APIPPAAPKLAGKAAAAPARPAAAAARAGAAVPKPSPAATAARSGLVYVQVGAFSTRE
                     RAETAARQVGGTVSPVGKLFRVRISGFASPAAAAAALAKAKGAGYSDARIQRAD"
     gene            2763..>3957
                     /locus_tag="VN627_03300"
     CDS             2763..>3957
                     /locus_tag="VN627_03300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HXD02311.1"
                     /translation="MIAGALALAVPLALTGAQVPAPQAAPAALGEEDAAAAAVAAERG
                     KAGAADLTDPNSPPASLAPIPLALLVDLGSGQTLFSREPDRRFMPASITKVMTLYVAF
                     ELISRGQLNENQTFRMSQEAWKHWHGVGSTMFLDREHDVTVHDLLMGIANVSANDGCI
                     VMAEGAAGSVPNWVALMNAEARRIGMTNSHYGTPNGWMDEGQTYVTPRDLATLAAAMI
                     RRHPDLYSRYIGHQTSTWNGITQHNHDPILGKLEGADGIKTGFTNQAGYGFLGSAIRN
                     GRRLVMVVAGVDRPKDRARAAADLMDWGFAAFESRPLFAAGAQVGSAKVQDGAAGSVP
                     LLAPTAYAATSPRGKREPVALRIVYDGPLPAPIRKGDAVASLEIKVGNGPAERIPLVA
                     GADVAK"
BASE COUNT          572 a         1422 c         1343 g          620 t
ORIGIN      
        1 tgggcgtggt gttcatcagc ggagcgacga agccgccgcc gggcacgtcg accaggacgt
       61 cgacccggcc cagcgcccgg tcgatctcgg caaagaacgc ctccatcgcc tcgggctcac
      121 gcacgtcggc atggaccagc agggtcttga ccggaaagcc ggagagttcg gcgcgcaccg
      181 cctcgatggc ggcggcatcg cgatcgcaca cggcaacatg catgcccgcc ctggcaaaat
      241 cgcgcgcgat cggccagccc aggccgccgg cgcctccggt gaccaccgca accttgccgg
      301 caaggccggc catgtcttcg aggaagccca tctatactct ccgctgtcgt gccggtgatc
      361 gccgtcgccg ttgccgctat ggccccgctg gcggacaggg tgggattcga acccacggtg
      421 agcttgcacc cacggcggtt ttcaagaccg ctgccttaaa ccactcggcc acctgtcccc
      481 aagcgaggtg aaacgcggct agcgccgcgt ggcggttttg tcacgcctcg tcgcgcctct
      541 ggcgacggat cgtgaaaact tggccggggc ggctttccca agcgacaggg cctgtgcaac
      601 aaggccgcca tgctgtcgtt gccccggtcc gtgcgtctcc ttggtgcttc acttgcctcg
      661 ctgatgctgg ccgtctcgcc ctcacagggg caggttgacg cagcaggcat cgcgacgtcg
      721 ggtccggcgg cgccgaccag cgccgaatcg gcggaagaag ccggctttcg gggctatctc
      781 cagctgctcg ccgcccgggc gcggggcgag ggggtgcgcg aatcgaccat tgccgcggtg
      841 acttcgggcc tcaccttcaa ttccctggtg atcgcgcttg atcgttcgca gccgggctct
      901 tcggccaatc cctcggcagc gcccgccttc gccccttacc gccggatgca tgtcgacgcc
      961 gcgcgtatcg cgggcggacg gcagatgcgc gccaatgtcg ccggcatcgc gcccgcgatc
     1021 gaggcgaaat acggcgtgcc ggtgcagatc ctgctggcga tctgggggca tgagaccaat
     1081 tacgggcgct ataccggcga cttcgacctg gctcgctcgc tggcgaccct ggcctacgag
     1141 ggccgccgcc gtgagctgtt tgccgacgaa ttcgtcgccc tgctcaagat ggccgaccgg
     1201 ggcgtgccgc tcgaccggct caagggctcc tgggccggcg cattcggcaa tccgcagttc
     1261 ctcccctcga tgtacctgcg cctcgccgtt gacggcgacg gcgatggcga ccgcgacatc
     1321 tggcacagcc gggccgatac cctcgcctcg atcgccaact atttccgcga tgccggctgg
     1381 cgggccgggc agccgtgggg cgtgccggcg ctggtgccgg ccgggtttga ccgcggcgtc
     1441 gtcacctccc ggctcaccgc ccccgattgc ccgcgcgtcc atgcccgcca tgcccgctgg
     1501 ctgaccgtgg ccgaatggcg ggcgaggggg gtgcaaacgc ttgccccgat cgccgatgac
     1561 gtcatggcca cgctgatcga acccgacggg ccggggcaga ccgcctacct gttaaccggg
     1621 aattatcggg tcatcctcga atataactgc tcgaactact acgcgctgtc cgtggggttg
     1681 cttgcagatg agattgcccg ttagccgtaa ccacggccac ccccgtcact tcggcctgtt
     1741 gctgctggcg actgtcatgt cgcttgccgg gctgctcgcc gcgccgctgc tggcgcgcca
     1801 gcccgccgcc ccggcgtctg cgccgaccgg tccggcgggc gactatccca tggtgcttgg
     1861 cgcgccctat gtgatcgacg gggcgaccta taccccggcg gacatcatga acgtcgacca
     1921 agtcggctat gccgtcatcg cgccggacgc cgggccgggc gtcaccatcg cccaccgcac
     1981 cctgccgctg ccgagttatg tcgaggtgac ctcgctcaag accgggcgga cgatcctcgc
     2041 ccgggtagag cggcgggggc cgatgagcgg gccggacctg atcgccctct cctccggggc
     2101 cgcggcgcag ctcggggccg atgggcggct gcccgtccgg gttcgacggg taaacccgcc
     2161 ggaaaccgaa cgcgccctgc tgcgcacggg gcagaccgcg ccggtgcgga tggatacgcc
     2221 gatgtcgctg gtgaacgtgc tgctgcgcaa gcttgatccg gcgctggtga cgccgccagt
     2281 gctgccagcg cctcccgctc cgcctgcgcc ggagcctgcc acgaccgatg ttgcccgcaa
     2341 ggcgccgata ccgccggccg cccccaagct ggccggcaag gcagccgccg cccctgcgcg
     2401 gcccgctgcg gcagctgccc gcgccggtgc ggccgtgccg aagccgtccc ctgccgcgac
     2461 agccgccagg tcaggcctcg tctatgtcca ggtcggcgct ttctccacgc gggaacgggc
     2521 cgaaactgcg gcgcggcagg tgggcggcac ggtttcgccg gtcggcaagc tgttccgtgt
     2581 gcggatcagc ggcttcgcca gccctgctgc ggcagccgcc gccctcgcca aggccaaggg
     2641 cgcgggttat agcgatgccc gaatccagcg ggctgactga ctgacgccag acttggcgca
     2701 agcgccggtc cgcctcggct cgaggttggg accggaacga cttgaaaaga ctgcctttcg
     2761 ccatgatcgc cggggccctt gcgctagccg taccgctggc gctgaccggg gcccaggtgc
     2821 ccgcgcctca ggccgctccg gccgccttgg gcgaggagga cgcggcggcc gctgccgtcg
     2881 cggccgagcg cggcaaggct ggcgctgccg acctgaccga tcccaacagc ccgcccgctt
     2941 cgctcgcgcc gatcccgctg gccctgctgg tcgatctcgg ttccgggcag accctgttct
     3001 cgcgcgagcc cgaccggcgg tttatgcccg cctcgatcac caaggtgatg accctatacg
     3061 tggccttcga gctgatcagt cgtgggcagc tgaacgaaaa ccagaccttc cggatgagcc
     3121 aggaggcgtg gaagcactgg cacggcgtcg gctcgaccat gtttctcgac cgcgagcatg
     3181 acgtcaccgt ccacgatctg ctgatgggta tcgccaatgt ctcggccaat gacggctgca
     3241 tcgtcatggc cgaaggcgcg gcgggatccg tgcccaactg ggtggcgctg atgaatgccg
     3301 aggcccgccg catcgggatg accaacagcc actatggcac gcccaacggc tggatggacg
     3361 aggggcagac ctacgttacc ccgcgcgatc ttgcgacgct cgccgccgcg atgatccgcc
     3421 gtcaccccga tctctacagc cgctatatcg gccaccagac ttccacctgg aacggaatca
     3481 cccagcacaa ccacgacccg atcctcggca aactcgaggg ggccgatggg atcaagaccg
     3541 gattcaccaa ccaggcgggc tacggttttc tcggttcggc gatccgcaat gggcgccggc
     3601 tggtcatggt cgtcgccggg gtggaccggc ccaaggaccg ggcccgcgcc gcagccgacc
     3661 tgatggactg gggctttgcc gcgttcgaga gccggccgct gtttgccgcc ggggcgcagg
     3721 tgggcagcgc gaaggtgcag gacggcgccg cggggtcggt cccgctgctg gcgcccacgg
     3781 cctatgcggc gaccagcccg cgcggcaagc gcgagccggt ggccctgcgc atcgtctatg
     3841 acggcccgct gcccgcgccg atccgcaagg gtgatgctgt cgcgtcgctg gaaatcaagg
     3901 tcggcaacgg cccggccgag cggatcccgc tggttgcggg ggccgacgtg gccaagg
//
LOCUS       DATNBV010000137         5202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4565531,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000137 DATNBV010000000
VERSION     DATNBV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4565531"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VN627_03305"
     CDS             complement(<1..385)
                     /locus_tag="VN627_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXD02312.1"
                     /translation="MPKDSNPQSKKSKESSKAGDNARPNKPGKPAKPRHAAAAVDALT
                     LLEADHREVDDLFGAFEEARGDAEQRDIAVSICVALKVHARLEEELFYPAIWPVLDDQ
                     SLIAEAMVEHASARDLIAQIESGAPG"
     gene            855..1589
                     /locus_tag="VN627_03310"
     CDS             855..1589
                     /locus_tag="VN627_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXD02313.1"
                     /translation="MSSSVDCLVIGAGPAGLTAAIYLARLHLSVRVIDAGPSRAALIP
                     RTRNHAGFPGGITGRDLLQRMRQQAEEFGTSLTTGVAEKLERSGDGFAIHSADGTMQA
                     LTVLLATGVVNKRPPIAPEFHDLALSRGLPRYCPICDGYEVTDQQVGGAVERGVHLGA
                     PHYGGVALQGSALQWERVAEERRDGPARSPNKERHGRVLRVRASRHGQSTANSRSGFI
                     PRTCDPSAFRPPAQTTPVRAGQLLWL"
     gene            complement(1561..1872)
                     /locus_tag="VN627_03315"
     CDS             complement(1561..1872)
                     /locus_tag="VN627_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02314.1"
                     /translation="MTSHLVQVFLPLSCQGGSPQPRAAFAAVRKELLGRRGGVTIYAR
                     APAEGLWQDDEGVDVDRIVIFEAMDDNFDPEWWTQYRTTLQSRFHQEEILVRAIATVR
                     L"
     gene            complement(1941..2249)
                     /locus_tag="VN627_03320"
     CDS             complement(1941..2249)
                     /locus_tag="VN627_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02315.1"
                     /translation="MKVRVFVPSLGSRGEDPVYCGSDHTFPGLPSLGQALRFTDHQRG
                     DFTVVNVGYVQDGDAFIPAVWLACSETQMGYAEPEGTSQIPEYRDLNYDVPPATMTTY
                     "
     gene            2448..2642
                     /locus_tag="VN627_03325"
     CDS             2448..2642
                     /locus_tag="VN627_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02316.1"
                     /translation="MDQDTANLIAALCTRAAMIMEDNVDNMLTMSQVDRVARTARLQQ
                     LRRACDHIVSLVDAALALDA"
     gene            complement(2643..2858)
                     /locus_tag="VN627_03330"
     CDS             complement(2643..2858)
                     /locus_tag="VN627_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXD02317.1"
                     /translation="MDIRTRLGHNIRQLREAKGWSQEDYADRAGIHRTYVSDIERGRR
                     NPTITVVEKLATPFGVSPGSLLDGPAS"
     gene            2975..4357
                     /locus_tag="VN627_03335"
     CDS             2975..4357
                     /locus_tag="VN627_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02318.1"
                     /translation="MTLVFLNGADAPPQIITRVPEGEARREDALRDLLFAHPAMLPVS
                     EIDPGIGALVPIARELNVPEVGRIDAFLVDPRGALVIVECKLWRNPQARREVVGQILD
                     YARALARFSYEDLQREVSRATGRKGNALYELVAAAHPGLDEARFVDRVSRNLAAGRFL
                     LLVVGDGIAEGTQRIGEYLVAQPGLAFDFALVEMAQYSWRDGMGTERTAVQPRLLAKT
                     VVIERAVIRNEASVVIADLADEPAAPTGEGRGRKLDGALQASWRRFAEQLIAGLRFDD
                     PAQPGLRIGGLGWLKAALPRGHWLTLYRASASGKIGAYVNLAGSEGHEVFQQLLAERA
                     TIAECLPRPGCPSPAGSAPPRAPPLPSPGPHRCRGTRRPKPNRPRASRRPPISWSTAS
                     ARGSTSSGPRGERASSIARRCGAAPGRDERDPAHQVGPRAAVAPFLHARLSLFRDRRG
                     PRPCQPAHRS"
     gene            4290..4862
                     /locus_tag="VN627_03340"
     CDS             4290..4862
                     /locus_tag="VN627_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02319.1"
                     /translation="MRDFLYSEIAAVHGLANLPTDPDLAIAAGKRLCEELLEPLQATF
                     GRIVIRSAYRSAELNAFGHRMARAGAEDYNCASNAHNQAVHIWDEYDAQGCWGAAACI
                     VVPSFWDRCRAPGDWQRLAWWVHDHLAYSHMTFFTTRFAFNLAWRSTPRRRISSHVAP
                     RGLLTCPCMPGHEGSHEAQWRGIVPEQPMA"
     gene            complement(4899..>5202)
                     /locus_tag="VN627_03345"
     CDS             complement(4899..>5202)
                     /locus_tag="VN627_03345"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02320.1"
                     /translation="RPLLVPVRYEKLARMEARAVMFEVGQVHVPRAAEWLGTYLEELL
                     SFPNSRKDDQVDSTSQALDYLQQRFAATLRSRPEGRKRPKGKPRPTGVPQRRVRAL"
BASE COUNT          877 a         1738 c         1693 g          894 t
ORIGIN      
        1 cccccggcgc gccggattcg atctgggcga tgaggtcgcg cgcgctggca tgctcgacca
       61 tcgcttccgc gatcaggctc tggtcgtcca gcacgggcca gatggcggga tagaaaagct
      121 cctcctccag ccgcgcatgg actttgagcg cgacacagat cgacacggct atatcgcgct
      181 gctcggcatc gcccctggcc tcttcaaagg caccgaacag gtcatccacc tcgcggtgat
      241 cggcctcgag cagcgtcagc gcgtcgaccg ccgcggcagc atggcgaggt ttggcgggct
      301 tgcctggctt attcgggcgg gcgttgtctc ctgctttgga cgattccttc gactttttgg
      361 attgcgggtt gctgtccttg ggcatggagg tctcctcagg cgttcagcga caacctcggt
      421 gcctgcccgc cgttccgctc ccgatccgtc cgccgcgacg gaatgccttg ccggttcgtc
      481 ggaaacggtt ccgccgcaga caagtgctgc gggaccactc gcgccggaac aggcgcggtc
      541 ttgcagcgct gtggtcagac gccagccaag caggcggaga cacaccatgc cgcaatcgca
      601 atccgctacc ctctatcgca tggtcctgcc cgaccacact tgcccgttcg gagtccgcgc
      661 caggcagctg ctggaagacg ccggataaga ggccgatgac cgcatcctgt cgtcgcgcga
      721 ggcagtcgac cagttccaag acgggcagga tgtcgacacc accccacagg tcttcattgg
      781 gcaggaacgg gtcggcgggt gcgacgatct cgaacgctat cttgccggtt gagccgaagg
      841 gatcgtcagt ttcaatgtca tcttctgttg actgccttgt catcggcgcc ggtccggccg
      901 gccttaccgc agcaatctac ctcgcccgct tgcacctctc ggttcgggtc attgacgccg
      961 gacccagccg tgcagcgctg attccccgca cgcgcaatca cgccggcttc ccgggcggca
     1021 tcaccggacg agacctgttg caacggatgc gccagcaagc cgaggaattc ggcacctcgc
     1081 ttacaaccgg cgtcgcggag aaactcgaac gcagcggcga tggcttcgcc atccactctg
     1141 ccgacggcac gatgcaggca cttaccgttc tgctcgccac cggcgtcgtc aacaagcgcc
     1201 cgccgattgc acctgagttt cacgacctgg ccttgtcacg cgggctgccg cgctactgcc
     1261 ccatctgcga tggctacgag gtgaccgacc agcaggttgg cggggcggtg gaacgaggag
     1321 ttcacctcgg tgcaccccat tacggcggcg tagcgctgca gggcagtgcc ctccaatggg
     1381 aacgcgtcgc ggaagagcgc cgggatggcc cagcccgcag tcccaataaa gagcggcacg
     1441 gacgagttct gcgggttcgg gccagtcgac atgggcaaag tacggccaat agcaggagcg
     1501 gtttcatccc gagaacctgc gaccccagcg cattccggcc accggctcag acaacaccgg
     1561 tcagagccgg acagttgcta tggctctgac gaggatctcc tcctggtgga accgcgactg
     1621 caaggtagtc cggtattgtg tccaccactc cggatcaaaa ttgtcgtcca tcgcctcgaa
     1681 gatgacgatg cgatccacgt caaccccctc atcatcctgc cacagacctt ccgccggcgc
     1741 gcgcgcgtag atcgtgacgc cgccgcggcg gccgagcagt tccttgcgga cggccgcgaa
     1801 tgccgcacgc ggctggggtg atccaccctg acaactaagg gggagaaaga cctgaacgag
     1861 gtgcgatgtc atatttgcct cgagccatcg cctaagttga gtgaatgggt catggacccg
     1921 agtatgccac ttccgaccgc tcagtacgtg gtcatggtcg cgggcggcac atcgtagttg
     1981 aggtcacgat attccgggat ctgcgaagtg ccttccggct cggcgtaccc catctgcgtc
     2041 tcactgcagg ccaaccagac cgccggtatg aaggcgtcgc catcctgcac gtaacctacg
     2101 ttcacaacgg taaagtcacc tcgctggtgg tcagtgaacc gcaaagcctg cccgaggctg
     2161 ggaaggcccg gaaacgtgtg gtcgcttccg caatagaccg ggtcctcacc acggctgcca
     2221 aggcttggca caaacacccg gactttcatc agaccgtcct cctctcccgg ctgaacggac
     2281 gaccagtatc gaccagcttg gtcgcaccag cgccgatgtc cattcaacga gcgagctgac
     2341 gcaagtgtcc aggtcccgtc tgaagtagcg tcatgactgc ccgaccgagc gaacggtgtg
     2401 aagctgcata tggacttgtg cctgggaacc gggctgtgtc catggccatg gaccaggaca
     2461 ccgcaaacct catcgccgcc ctgtgcacgc gggccgccat gatcatggaa gataacgtcg
     2521 acaacatgct gaccatgagc caggtcgatc gggtcgcgcg cactgcccgg ctgcagcagc
     2581 tgaggcgggc gtgtgaccac atcgtgagcc tggttgacgc tgccttggcg ttggacgcct
     2641 gatcagctcg caggaccgtc cagcaggctg ccgggcgaca ccccgaacgg cgtagccagc
     2701 ttctcgacca ctgtaatcgt cggattgcgc cgcccgcgct cgatgtcgct gacataggtg
     2761 cggtggatgc ctgcccggtc ggcatagtcc tcctggctcc agcccttggc ttcgcggagc
     2821 tgacgaatgt tgtgaccgag gcgcgtgcgg atatccacgc tgggctaaag acagccatgt
     2881 cgctgatcta tctacagacg atcagtgaca ttggcgttga cttggggcgc cacccgagca
     2941 tgtgtcgtgt cactgataaa cgacaggtga gccgatgacc ctggtctttt tgaatggtgc
     3001 ggatgcaccc ccgcagatca tcacgcgggt gccggagggc gaagcccggc gcgaagatgc
     3061 gctgcgcgac ctgctgtttg cccatcccgc catgctcccg gtcagcgaga tcgatccggg
     3121 gatcggcgcg ctggtgccga tcgcgcgcga gctcaatgtc cccgaggtcg gccggatcga
     3181 tgccttcctg gtcgatccgc gcggcgcatt ggtgatcgtt gaatgcaagc tgtggcgtaa
     3241 cccgcaggcg cggcgcgaag tggtcggcca gatcctcgat tatgcccggg cgcttgcccg
     3301 gttcagctac gaggacctcc agcgcgaggt ctcgcgggcg acgggccgca agggcaatgc
     3361 gctctatgag ctggtcgcag cagcgcatcc cgggctcgac gaagcgcggt tcgtcgaccg
     3421 ggtctcgcgc aaccttgccg ccgggcggtt cctgctgctg gtggtgggcg acgggattgc
     3481 cgaaggcacc cagcggatcg gcgagtacct ggtggcccag cccggtctcg ccttcgattt
     3541 tgccctggtc gagatggccc agtacagctg gcgcgatggc atggggaccg agcggaccgc
     3601 ggtgcagccg cggctgctcg ccaagaccgt ggtgatcgag cgggcggtga tccgcaacga
     3661 ggcgagcgtg gtcattgccg atctcgccga cgagccggcg gcgcctacgg gcgaaggacg
     3721 ggggcgcaag ctcgatggcg ccttgcaggc gagctggcgc cgctttgcgg aacagctcat
     3781 cgccgggctc aggttcgatg atccggcgca gccgggcctg cgcatcggcg ggctcggctg
     3841 gctcaaggcc gcgctgccgc gcgggcactg gctgaccctc taccgggcga gtgcgagcgg
     3901 caagatcggg gcctatgtca accttgccgg aagcgagggg cacgaagtgt tccagcagct
     3961 gctcgccgag cgggcgacga ttgccgagtg tttgccgagg ccgggctgcc cgagcccagc
     4021 tgggagcgca ccgccaaggg cgccaccatt gccctcacct ggcccgcacc gctgccgtgg
     4081 gacgaggcgg ccgaagccga acaggccgcg cgcctcgcgc aggccgccaa tcagctggtc
     4141 aacagcttcc gcccgcggct cgaccagctc gggaccgcgg ggtgagcggg ccagcagcat
     4201 cgccagacgc tgcggcgccg cgcccggccg cgatgagcgt gacccagctc accaagttgg
     4261 gccgcgagcg gctgtcgcgc catttcttca tgcgcgactt tctctattcc gagatcgccg
     4321 cggtccacgg ccttgccaac ctgcccaccg atcctgacct cgcgatcgct gccggcaagc
     4381 ggctgtgcga ggagctgctc gagcccctcc aggcgacgtt cggccggatc gtgatccgct
     4441 cggcctatcg ctcggccgag ctcaatgcct tcggccaccg gatggctcgc gctggcgccg
     4501 aggattacaa ctgcgccagc aatgcccaca atcaggcggt gcacatctgg gacgagtacg
     4561 atgcgcaagg ctgttggggc gcggccgcct gcattgtcgt gccgagcttc tgggaccggt
     4621 gccgcgcgcc cggcgactgg cagcggctcg cctggtgggt ccacgatcac ctggcctatt
     4681 cccacatgac cttcttcacc acccggtttg cgttcaacct tgcctggcgc agcacgccgc
     4741 ggcggcggat ctccagccat gtcgccccgc gaggcctgct gacctgcccg tgcatgccgg
     4801 ggcacgaggg cagccacgag gcgcagtggc gcgggatcgt gcctgagcaa ccaatggcgt
     4861 gacctgctcc actccatggc aactgaccgt gaccggggtc atagcgcgcg cacccggcgc
     4921 tgcggcacac ccgtcggccg gggcttgccc ttcgggcgct tgcggccctc tggacggctt
     4981 cgtagcgtgg cggcaaagcg ctgctgcagg taatcgaggg cctgcgaggt actgtcgacc
     5041 tgatcgtcct tgcggctgtt ggggaaggat agcagctcct caaggtaggt cccaagccac
     5101 tcggccgcgc gcggcacatg gacctggccg acctcgaaca taaccgcgcg ggcttccata
     5161 cgcgcgagct tctcgtagcg caccggaaca agcaacggcc gg
//
LOCUS       DATNBV010000138         4972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3275369,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000138 DATNBV010000000
VERSION     DATNBV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3275369"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..651
                     /locus_tag="VN627_03350"
     CDS             307..651
                     /locus_tag="VN627_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HXD02321.1"
                     /translation="MNPTLLFPPLLLAGSNVLMNLAWYWHLKLPREAMWAAILISWGI
                     ALFEYCLAVPANRIGASRYSLYELKTMQEVFSLSGFVLVAWMLFGQRPGLPQVAGFAL
                     ILVGASLVFKGN"
     gene            653..1834
                     /locus_tag="VN627_03355"
     CDS             653..1834
                     /locus_tag="VN627_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HXD02322.1"
                     /translation="METRAGDPGAFAQFDLTRVDSPAFVVDAARLRQNLRILADVGER
                     SGARVLGALKAFSMWSVAPIIGEYLDGVCTSGLWEARLAGEFYDGEIATYCAAYKAED
                     LPEILRLSDHVIFNSPQQIERFQPLIEAARAEGQQFDIGLRINPLHAEGEVPRYDPCA
                     PHSRLGFPLDQLTAEHMALIDGIHMHTLCEQDFAPLARTWDVVFDKLEPFFGEFSWIN
                     LGGGHHITRADYQREELIEFLRDMAEDTGAQVYLEPGEAVALDAGILVGTVLDVSWND
                     MPVAITDISATCHMPDVIEAPYRPAMLGEREPEEEGEAVRLGGPSCLAGDVIGDYVLP
                     EPPEPGVRFAFLDQAHYSMVKTTTFNGVPLPSIWLWDSETDQLDCVKRFGWETFRDRL
                     S"
     gene            complement(1837..3231)
                     /locus_tag="VN627_03360"
     CDS             complement(1837..3231)
                     /locus_tag="VN627_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HXD02323.1"
                     /translation="MRLSEQGACLPSPALMERAGLCIGCGSCASREAGARMGFDSAGF
                     LKPRGAREWHRRPSPVLARTCPFSPHAHDETELAARLFPDTFIDPWIGRYRATWVGAA
                     DPATRAEGSSGGMATWLATEMLEQGEVDGVAHVVPTPADSGRLFAYTLSRSPEAVRRG
                     AKSRYYPIDLDHVLREIRATPGRYAVVGIPCFIKAIQLARTEEPLLEERIVATIGLFC
                     GHMKSARLARSFARQLGIAEDDLAAIDYRHKDPARPANWYRAEVHSRGGEVRQQDWWH
                     LAEGDWGAGFFMSPACNWCDDVAAECADVALGDAWLEPYSSDGRGTNVVVVRSGMMAR
                     LIGRGMAEGRLDLAAVDAEFVRRTQAAGFRQRREGLGWRLARAGRRRAIVPRKRVEPD
                     VAIPARRRAIYAMRMAISRWSVRVDRLAETLRAPGLYYGWARCVSAIYHALAYSRGAL
                     GRLADRVWPAAGDG"
     gene            complement(3218..4318)
                     /locus_tag="VN627_03365"
     CDS             complement(3218..4318)
                     /locus_tag="VN627_03365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HXD02324.1"
                     /translation="MRHRDRLDLQGAILSDGPAGRRLRLGVLTFHRCVNYGSYWQARC
                     LVDGLRRMGHEVVVLDHHDARIVRREWACALQPQLPLRSAQSDRRRHAAKVRGFARAQ
                     AQLPLSAPFPLTGAPQEPLDAVIVGADEVWNPSHPWYGGAGLFWGERLEAVPLVAHAV
                     SCGNHDAPFPADRAARLGRFASIAVRDVTTRARVHEATGTSPPLVLDPCLQFPPEPAS
                     PLRRAPYALVYGHDFPDWAGLAAREWAGQRGLQLLSIGYRNAFADCQWLEANPGGFAS
                     AVAGAAAVITTMFHGTVFALNARLPFAVIPSDYRATKLTDLAGLLGAERHLVRTPDAL
                     PTALAEPPGADVIGAIAAMRASSHATLRHALV"
     gene            complement(4319..4624)
                     /locus_tag="VN627_03370"
     CDS             complement(4319..4624)
                     /locus_tag="VN627_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02325.1"
                     /translation="MSSAQRTTDHDAIRKWIEARKGRPSIVRATGDAGGSKGGRKGGL
                     LRIDFGEKDEALEEIDWDEFFQIFDDNDLEFLHQDKTADGKQSRFSKFVAAGSDDDK"
     gene            4662..>4972
                     /locus_tag="VN627_03375"
     CDS             4662..>4972
                     /locus_tag="VN627_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HXD02326.1"
                     /translation="MASPRFRSGRPPRSGEGAARRTDWRVDLSPGVGLGAAMTIAPAT
                     DEDQAALRLAAEVPDTALCTIVGIDGSYSRRRGAQLAVAPDGRMAGSLADGCLEKELA
                     T"
BASE COUNT          792 a         1738 c         1649 g          793 t
ORIGIN      
        1 catgacatgg gagatcggcc cgagcgagac agtgcgctgc gcgtgcttgg tcttgaagtg
       61 ttgcggatcc ccgccgccga cgtgctgaaa tcgccagagg atatcggcga agcgctggtg
      121 cgttattgca agcggtgaat acccttccgt cagtctcgct acgcgcgctg ccacctcccc
      181 atttgtcctg cggaaaaatg gagaggatcg agcgggccgg gaccctgtcc attttcacga
      241 agtgcaaatg gggaggtggc agcgcgaagc gctgacggag gggccttttt gcgctacccg
      301 cgcctgatga acccgaccct ccttttcccg ccgttgctcc ttgccggttc caacgtgctg
      361 atgaacctcg cctggtactg gcacctcaag ctcccgcgcg aggcgatgtg ggcggcgatc
      421 ctcatttcgt ggggtattgc cctgttcgaa tactgcctcg ccgtgcccgc caaccgcatc
      481 ggcgcgagcc gctattcgct ctatgaattg aagaccatgc aggaagtctt ctcgctcagc
      541 ggcttcgtac tggtcgcgtg gatgctgttc gggcaaaggc cggggctgcc gcaggtggcg
      601 ggattcgcgc tgatcctcgt cggtgcgtcg ctggtcttca aggggaactg acatggaaac
      661 cagagctggc gatccgggcg ccttcgccca gttcgacctc acccgcgtcg acagccccgc
      721 cttcgtggtc gacgccgcgc ggctgcggca gaacctgcgc atccttgccg acgtgggcga
      781 acgctcgggc gccagggtgc tgggggcgct caaggcgttc tcgatgtggt ccgtggcccc
      841 gatcatcggc gaatacctag atggcgtgtg cacctcgggc ctgtgggagg cgcgccttgc
      901 cggcgagttc tacgatggcg agatcgccac ctactgcgcc gcctacaagg ccgaggacct
      961 gcccgaaatc ctgcgcctgt ccgatcacgt gatcttcaat tcgccgcagc agatcgagcg
     1021 gttccagccc ttgatcgagg ccgcgcgggc cgagggccag cagttcgaca tcggcctgcg
     1081 aattaacccg ctccacgccg agggcgaggt tccccgctac gacccttgcg ccccgcattc
     1141 ccggctcggt ttcccgctgg accagctgac ggcagagcac atggccctga tcgacgggat
     1201 ccacatgcac accctgtgcg agcaggactt cgcgccgctg gcacgcacct gggacgtggt
     1261 gttcgacaag ctcgagccgt tcttcggcga gttctcctgg atcaatctcg gcggcggcca
     1321 ccacatcacc cgcgcggatt accagcgcga ggaactgatc gaattcctgc gcgacatggc
     1381 cgaggatacc ggtgcccagg tctatctcga accgggcgag gccgtcgcgc tcgacgccgg
     1441 cattctcgtg ggcaccgtgc tcgacgtcag ctggaacgac atgccggtgg ccatcaccga
     1501 catctcggcc acctgtcaca tgcccgacgt gatcgaagcg ccctatcgcc cggccatgct
     1561 cggcgagcgc gagcccgagg aagagggcga agccgtgcgc ctcggcggcc cctcgtgcct
     1621 cgccggtgac gtgatcgggg actatgtcct gcccgaaccg cccgaacccg gcgtgcgctt
     1681 cgccttcctc gaccaggcac actattcgat ggtcaagacc acgaccttca acggagtgcc
     1741 gctgccttcg atctggctgt gggattcgga gaccgatcaa ctcgattgcg tcaagcgctt
     1801 cggctgggaa accttccgcg accgcctgag ctgacgctag ccgtcgcccg cggcaggcca
     1861 cacccgatcg gccagccggc ccagcgcccc gcgcgaatag gcgagggcgt ggtagatcgc
     1921 ggaaacgcag cgcgcccagc catagtagag ccccggcgcg cgcagcgttt cggccaaccg
     1981 gtccacccgc accgaccagc ggctgatcgc catgcgcatg gcatagatcg cccgcctcct
     2041 cgccggaatg gccacgtcag gctccacccg cttgcgcggc acaatggccc tccgccggcc
     2101 cgctcgggcc aagcgccagc ccagcccctc gcgacgctga cgaaagccgg ccgcctgggt
     2161 ccggcgcacg aattccgcgt cgaccgccgc aaggtccagc cgcccctccg ccatgcccct
     2221 tccgatcagc cgcgccatca tgcctgagcg gacgaccacc acgttggtcc cccgcccatc
     2281 ggaggaatag ggctcgagcc aggcgtcccc cagcgccacg tcggcgcatt cggcggcaac
     2341 gtcgtcgcac cagttgcagg ccgggctcat gaagaaaccc gcgccccagt caccctcggc
     2401 gaggtgccac cagtcctgct gccggacctc gccaccgcgg gaatgcacct cggcgcggta
     2461 ccagttggcc gggcgggccg ggtccttgtg gcgatagtcg atcgcggcga gatcgtcttc
     2521 cgcaatgcca agctgccggg caaagctacg ggcgagccgt gccgatttca tgtgcccgca
     2581 gaacagaccg atagtggcaa cgatccgctc ttccagcagc ggctcttccg ttcgggcaag
     2641 ctggatcgcc ttgatgaagc agggtatccc caccacggca tagcgccccg gcgtcgcccg
     2701 gatttcgcgc agcacatggt cgagatcgat cggatagtag cgggatttcg ccccgcgccg
     2761 cacggcttcc gggctgcgcg agagggtgta ggcgaacagc cggccgctgt ccgccggcgt
     2821 cggcaccacg tgggcgacgc cgtccacttc gccctgttcg agcatttcgg tcgcgagcca
     2881 ggtggccatg ccgccgctgc tgccttcggc cctggtcgca ggatcggcag cgccgaccca
     2941 ggttgcccgg tagcgaccga tccatggatc gatgaaggtg tccggaaaca gcctggcggc
     3001 cagctcggtt tcgtcatgcg cgtggggtga aaacggacag gtccgcgcca gcacgggcga
     3061 gggtcgccga tgccactcgc gcgccccacg cggcttgagg aacccggcgc tgtcgaaccc
     3121 catgcgcgcg cccgcctcgc gcgaggcgca ggacccgcag ccgatgcaca ggcccgcccg
     3181 ctccatcagg gccggggagg ggagacacgc gccctgctca gacaagcgca tggcgcaagg
     3241 tcgcgtgcga cgaggcgcgc atagcggcga tcgcgccgat cacgtcggcc cctggcggtt
     3301 cggccaaggc cgttggcagg gcatccggcg tgcgcaccag atgccgctcg gcgcccagca
     3361 gcccggcaag gtcggtcagc ttcgtcgccc ggtagtcaga tgggatcacg gcaaatggca
     3421 ggcgggcgtt cagcgcgaaa accgtgccgt ggaacatggt ggtgatcacc gcggccgccc
     3481 cggcgacggc gctggcgaat ccgccggggt tggcttcaag ccattggcaa tcggcaaacg
     3541 cattgcggta gccgatgctg agcaattgca atccccgctg ccccgcccat tccctggccg
     3601 cgagtcccgc ccagtccgga aagtcatgcc catagaccag cgcatagggc gcgcggcgca
     3661 gcggcgaggc gggctcgggc gggaactgca ggcaggggtc gagcaccagc ggcggtgagg
     3721 tgccggtggc ttcgtgcacg cgggcccggg tcgtcacgtc gcgcacggcg atgctggcaa
     3781 accgcccgag ccgcgcggcg cggtcggcgg ggaaaggcgc gtcgtggttt ccgcaggata
     3841 cggcgtgggc cacgagtggc accgcctcca gccgctcgcc ccagaacaga cccgcgccgc
     3901 cataccaggg gtggctgggg ttccacacct catcagcgcc aacgatgacg gcgtccagag
     3961 gctcttgcgg cgcaccggtc agcggaaacg gcgccgagag cggaagctgc gcctgcgccc
     4021 gcgcgaagcc gcgcaccttg gccgcgtgcc gacgccgatc ggactgcgcg gaacgcagcg
     4081 gcaattgcgg ctggagggcg caggcccatt ccctccggac tattcgcgcg tcgtggtgat
     4141 cgaggaccac cacctcgtgg cccatccggc gcaaaccgtc gaccaggcat cgcgcctgcc
     4201 agtaggaacc atagttgacg cagcgatgga acgtcagcac gcccaggcgc agccgacggc
     4261 cggccgggcc gtcggacagg atggctccct gcaggtcgag acggtcgcgg tggcgcactt
     4321 atttgtcgtc gtccgaaccg gcggcaacga acttgctgaa ccggctctgc ttgccgtcgg
     4381 cggtcttgtc ctgatgaagg aactcgaggt cgttatcgtc gaagatctgg aagaattcgt
     4441 cccagtcgat ctcctccagc gcctcgtcct tctcaccgaa gtcgatgcgc agcaggccgc
     4501 ccttgcggcc ccccttcgaa ccgccggcgt cgccggtagc gcggacgatg ctcggccggc
     4561 ccttgcgcgc ttcgatccac ttgcggatcg cgtcgtgatc ggtggtgcgc tgcgccgaac
     4621 tcatggcctt gctcctctgt tgtccgcctc acgcaatcgc catggccagc ccccggttcc
     4681 gcagtggtcg tccgccacga tcaggcgagg gcgcggcgcg gcggacggat tggcgcgttg
     4741 acctttcccc cggcgtgggc ttgggtgcgg cgatgacgat cgcgcccgcc accgatgaag
     4801 accaggccgc cttgcgcctc gccgccgagg tgccggacac ggcactgtgc accatcgtcg
     4861 ggatcgatgg ttcctattcg cgccgccgcg gtgcgcagct ggccgtagct ccggatggga
     4921 ggatggcggg cagccttgcc gatggctgcc ttgaaaagga acttgcgacc ca
//
LOCUS       DATNBV010000139         2486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2948970,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000139 DATNBV010000000
VERSION     DATNBV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2948970"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1565)
                     /locus_tag="VN627_03380"
     CDS             complement(123..1565)
                     /locus_tag="VN627_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD02327.1"
                     /translation="MAEHLLKFVNLPKAMPDKREAGLRRADFQEIYDRFRPAAAAAQA
                     SRCSQCGVPFCQVHCPLNNNIPDWLKLTAEGRLEEAYEIASATNTFPEICGRICPQDR
                     LCEGNCVIEQSGHGTVTIGSIERYITDTAFEMGWVKPLKPVRERPESIGIVGAGPAGL
                     AAAEFLRRKGYQVTVYDRYDRIGGLMIYGIPNFKLEKPIVLRRAEQLVQGGIQFRLNC
                     ELGRDVTLAELRSKHDAVLLAIGVYKAREMAGPGSGLDNIVPALSYLTASNRSGLGER
                     VPDFESGALDAKGKRVVVIGGGDTAMDCVRTAVRQGAISVHCVYRRDRANMPGSQREV
                     AHAEEEGVQFVWQAQPEAFLGNADNRVTGVRCQRIHLGLPDATGRQTPRVIEGSGFTI
                     EADLAIKALGFDPEDVAGMTGSRDLSLTNWGTLKIDWRTLMTSQPGVFAAGDIVRGAS
                     LVVWAIRDGRDAAASIDKYLQSAAQRVAAE"
     gene            1833..>2486
                     /locus_tag="VN627_03385"
     CDS             1833..>2486
                     /locus_tag="VN627_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HXD02328.1"
                     /translation="MPNIDILVLAILQGVTEFLPVGSSGHLLMVPQLYCWGPQAPGLE
                     LAMLLGALLAVVVCFLGNLLGMMQGFFKVLQGKRDGRVHLIGLLLVAAIPYLVAAFLV
                     EHYAGDALRSPLVIGNALVWFGLLLYGADKLGLTVRRIEHMTFGQALIIGLIQCFAVI
                     PGAGGTGITMTIGRVLGYERPHAVRFAFLLSIPILAVIAGYKGWLLFEAGAAVDFAQA
                     "
BASE COUNT          393 a          780 c          832 g          481 t
ORIGIN      
        1 aagccgcgat catgccgacg ccgcaggcat cgtgctcgtc ggccggatcg tagaggccat
       61 gggccgcgag gtgccgcgcg gtgttctgcc aaagttgtgc gaggtcggtc atgcttggcc
      121 cctcattccg cggcgacgcg ctgcgccgca ctttgcagat atttgtcgat gctggccgcg
      181 gcgtcccgcc cgtcgcggat cgcccagacc accagcgagg cgccgcgaac gatgtcgcct
      241 gccgcgaata cgccgggctg gctggtcatc aaggtgcgcc aatcgatctt cagcgtgccc
      301 cagttggtca gtgagaggtc gcggctgccg gtcattcccg ccacatcctc gggatcgaag
      361 ccgagcgcct tgatggcgag gtcggcttcg atggtgaagc cggatccctc gatcacccgc
      421 ggggtctggc ggccggtggc atcgggcagg ccgagatgga tgcgctggca ccggacgccg
      481 gtgacgcgat tgtcggcgtt gccgaggaag gcttcgggct gggcctgcca gacgaactgc
      541 acgccttctt cctcggcatg cgccacctcg cgctgcgagc ccggcatgtt ggcgcggtcg
      601 cggcgataga cgcagtgcac agaaatggcg ccctggcgca cggctgtgcg cacgcaatcc
      661 atcgcggtgt cgccgccgcc gataacgacc acgcgcttgc ccttggcgtc gagcgcgccc
      721 gactcgaagt ccggcacgcg ctcgccgagg ccgctgcggt tgctggcggt caaataggag
      781 agggcgggca cgatgttgtc gaggccactg cccggccccg ccatctcgcg tgccttgtag
      841 acgccgatcg ccagcagcac ggcatcgtgc ttgctccgca gctcggcgag tgtgacgtcg
      901 cggcccagct cgcaattgag cctgaactgg atgccgccct ggaccaattg ctcggcccgg
      961 cgcagcacga tcggcttttc cagcttgaag ttgggaatgc cgtagatcat cagcccgccg
     1021 atccggtcat agcggtcata gaccgtcacc tgatagccct tgcggcgaag gaattccgcg
     1081 gcggcgagac ccgcgggccc ggccccgacg atgccgatgg attcgggccg ttcgcgcacc
     1141 ggcttgagcg gcttcaccca gcccatctcg aaggcggtgt cggtgatgta gcgctcgatc
     1201 gagccgatgg tgacggtccc gtggccggac tgctcgatca cgcaattgcc ttcgcacagc
     1261 cggtcctgcg ggcagatgcg cccgcaaatc tccgggaagg tgttggtggc ggaggcgatc
     1321 tcataggcct cttccagccg cccctcggcg gtcagtttca gccagtcggg gatgttgttg
     1381 ttgagcgggc aatggacctg gcagaacggc acgccgcatt gcgagcagcg gctcgcttgg
     1441 gcggcggcgg cggccggacg gaaccggtca tagatctcct ggaagtcggc acgcctgagg
     1501 ccggcctccc gcttatccgg catggccttg gggagattga caaatttcag cagatgctcg
     1561 gccatcctgc gcccgtccca gccctaattg cggcgaaggt tctcagaaat cggcggcaga
     1621 ttaggtcaaa atacgcgacc aatccagcag tttttctcca ttaggacagt taatatgacg
     1681 tattataacg aagcagcctc gaaagtgacg ggcggagcac agcgattgcg gcatctaggt
     1741 tacaattagt accgttatgc gactatatgt tgcgtttttc cagcgaatcc aacgtgctat
     1801 aagctccggc gacatagcgg ggatcctacc gagtgccgaa tatcgacatc ctggtcctgg
     1861 cgattctcca gggcgtgacg gaatttctgc cagttggttc cagcggccac cttctcatgg
     1921 tgccgcaact ctattgctgg ggtccccagg cgccgggcct ggagctggcc atgctgctgg
     1981 gcgccttatt ggccgtcgtg gtgtgcttcc tcggcaatct gctcggcatg atgcagggct
     2041 ttttcaaggt cctgcagggc aagcgcgacg gccgcgtgca ccttatcggg ttgctgctgg
     2101 tggcggcgat tccctatctg gtggcggcgt tcctggtgga gcattacgcg ggcgacgccc
     2161 tgcgcagccc gctggtgatc ggcaacgcgc tcgtgtggtt tggcctgctg ctctatggcg
     2221 cggacaagct ggggctgacg gtgcggcgga tcgagcacat gaccttcggg caagccctga
     2281 tcatcggcct catccaatgc ttcgcggtca ttcccggcgc cggcggaacg ggcatcacca
     2341 tgacgatcgg acgggtgctc ggatatgagc ggccccacgc ggtacgcttc gcgttcctgc
     2401 tgtcgattcc gatcctggcc gtgatcgcgg gctataaggg ctggctgctg ttcgaggcgg
     2461 gcgccgcggt cgatttcgcg caggct
//
LOCUS       DATNBV010000140         3127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3592194,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000140 DATNBV010000000
VERSION     DATNBV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3592194"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..852
                     /locus_tag="VN627_03390"
     CDS             163..852
                     /locus_tag="VN627_03390"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HXD02329.1"
                     /translation="MNPNSADPAKVAPLKDRTALARLGEDVRRRLAADPAGYRVPTDK
                     AEIWAFAEFLSRDECERFMAIVDGVARPSAVFDLTYDAGYRTSYSGDVDRADPFVRMI
                     ERRIDDLLGIDPTCGESIQGQRYAVGQEFQAHLDWFWTLADYWPEQERRGGQRSWTAM
                     AYLNEVEEGGRTEFTRVGVSIPPQPGLLLVWNNALPDGSPNWDTMHAAHPVVRGTKYV
                     FTKWYRTRAWG"
     gene            860..2701
                     /locus_tag="VN627_03395"
     CDS             860..2701
                     /locus_tag="VN627_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXD02330.1"
                     /translation="MTFQFRGARPGLRAALLPLILLCGSVPAFGLAAPAKPSAQSDAA
                     EDRRLLALLADDARRSEALDPVDRIYRGDAVAPDALAEVFSAELSRKQLASVRQSLAA
                     LARIDRRRLSAERQLSYDAFAWDKRTALAFLQPDLRALTEVQPMNHFDGLQTAFPAIM
                     ARGGSLRYDTAADYRTNLGLMRAIAPVLDQAIVRFREGMASGVVEPRLTVRNMIGQID
                     AMLALGLAGSPFVSPVRDFPPGVSAADRAVLREAYLATTRDSVIPAYRRLRRFLADDY
                     LPVARETIGLSQMPGGAALYRALIRQHTTLPLDPAQVHQLGLAEVARIQQEMVGVEGE
                     LGYSVPLRAFFDVLRTDPRFHPSSAADLARGYARIAGEVDAQVPRFFLRVPRTRLAIE
                     PYPAYRARYEAGGSYNQGSADGTRPGVFFYNTYDLPSRYLTGMTTLFLHEGSPGHHFQ
                     ISLAQEDASLPNFQRFGGNTAYVEGWALYAETLGYPMGLYNDPMQHWGTLDDEMLRAM
                     RLVVDTGIHTMGWSRDQAIDYMLANSGMGRTDAVAEVERYIAIPGQALAYKIGGMTIQ
                     RLRAKAERELGDRFDIRAFHEQVLGSGALPLPVLEAKIDRWIAGKQG"
     gene            2774..>3127
                     /locus_tag="VN627_03400"
     CDS             2774..>3127
                     /locus_tag="VN627_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02331.1"
                     /translation="MSVTVAALYRFARFADPAALRGPLDELARAEGIKGTLLLAGEGI
                     NGTIAGSAAGIAAVLGHIRALPDCAELDVKFSSAPDMPFHRLKVRLKREIVTMGEPGI
                     DPRATVGTYVESQDWN"
BASE COUNT          488 a         1052 c         1052 g          535 t
ORIGIN      
        1 gacttcttct ggtaggtttt tgccccttcc tgccagaatg ccggtgagtg gaccggcgaa
       61 ctggcccccg tcccaccgtg gacgggggcc tttttcgtgg atcgatcgca cggggcggca
      121 attcggcttg tctcgcccgc cgcgcgctct agcgtgacgg gaatgaatcc gaattccgcc
      181 gatccggcca aggtggcccc gctgaaggac cggaccgccc tcgcccggct gggtgaagac
      241 gtgcgccggc ggttggcggc cgatcccgcc ggctaccggg tgccgactga caaggctgag
      301 atctgggcct tcgccgaatt cctcagccgg gacgagtgcg agcggttcat ggcgatcgtc
      361 gacggggtgg cccggccgtc cgccgtgttc gacctgacct acgatgcagg ctatcgcacc
      421 tcctattcgg gcgacgtcga ccgcgccgat cccttcgtgc ggatgatcga gcggcggatc
      481 gacgatcttc tggggatcga tcccacctgc ggggaaagca tccaggggca gcggtacgcg
      541 gttggacagg aattccaggc gcatctcgac tggttctgga cgctcgccga ttactggccc
      601 gagcaggaac ggcgcggcgg gcaacgctcg tggacggcaa tggcctatct caacgaagtc
      661 gaggaaggcg ggcgcacgga gttcacccgc gtcggcgttt ccattccgcc gcaaccgggg
      721 ctgctgctgg tgtggaacaa tgccttgccc gatgggtctc ccaactggga caccatgcat
      781 gcggcccacc cggtcgtgcg cgggaccaag tatgttttca ccaagtggta ccggacacgc
      841 gcgtggggct gacggggcaa tgacattcca attccgcggc gcccggccgg gcctgcgcgc
      901 cgccttgctc ccgctgatcc tgctgtgcgg gagcgtgccg gcatttgggc tcgcggcgcc
      961 ggcgaagcca tccgctcagt ccgatgcggc ggaggatcgc cgcctgctgg ccctgctggc
     1021 agacgatgca cggcgcagcg aggcgctcga tccggtggac cggatctacc gcggcgatgc
     1081 ggtcgcgccc gatgcgctgg cggaagtgtt cagcgccgaa ctgagccgaa agcagctggc
     1141 ctcggtgcgc cagagccttg ccgccctcgc ccggattgac cggcgtcgcc tgtcggccga
     1201 acggcagctc agctacgatg ccttcgcctg ggacaagcgc acggcactcg cctttcttca
     1261 gccggacctg cgtgcgctga ccgaagttca gccgatgaac catttcgatg ggctgcagac
     1321 cgccttcccg gcgatcatgg cccggggcgg gtcgctgcgc tatgataccg ccgccgatta
     1381 ccgcaccaat ctcggcctga tgcgcgcgat cgcccccgtc ctggaccagg ccatcgtgcg
     1441 cttccgcgag ggcatggcca gcggggtggt cgagccgagg ctcaccgtgc gcaacatgat
     1501 cggccagatc gacgcgatgc tggcgctggg gctggcgggt tcccccttcg tctcgccggt
     1561 gcgcgacttt ccgcccggag tgagcgcagc cgatcgggcc gtgctgcgcg aggcctatct
     1621 ggcaacgacg cgagacagcg tgatccccgc ctatcggcgc ctccgccggt tcctcgccga
     1681 cgactatctt cccgtggccc gcgaaaccat cggcctcagc cagatgcccg gtggagctgc
     1741 gctgtatcgt gccctgatcc gccagcacac caccctgccg ctcgatccgg cgcaggtgca
     1801 ccagctgggc ctcgcagagg tcgcgcggat ccagcaagag atggtcggcg tggagggaga
     1861 gcttggctac tcggtgcccc tgcgcgcctt cttcgacgtg ctgcggactg accctcgctt
     1921 ccatccgtcc agtgcggcgg accttgcgcg cggctatgcg cggatcgccg gggaggttga
     1981 cgcgcaggtc ccgcgctttt tcctgcgcgt gcccagaacc cgcctcgcga tcgaacccta
     2041 tcccgcctac cgcgcccgct atgaggccgg cggcagctac aaccagggca gcgccgacgg
     2101 cacccggccg ggcgtgttct tctacaacac ctatgacctg cccagccgtt atctcaccgg
     2161 catgaccacg ctgttcctcc acgaaggctc gcccgggcac catttccaga tcagccttgc
     2221 ccaggaagat gcgagcctgc ccaatttcca gcggttcggg gggaacacgg cctatgtcga
     2281 gggctgggcg ctctatgccg agacgctcgg ctatccgatg gggctctaca acgacccgat
     2341 gcagcactgg ggcacgctgg acgatgagat gctgcgggcc atgcggctgg tggtggatac
     2401 cggcatccac accatgggct ggagccgcga ccaggcgatc gattacatgc tggccaattc
     2461 cgggatgggg cggacggatg ccgtggccga agtggagcgc tacatcgcca ttcccgggca
     2521 ggcgctggcc tacaagatcg gtggcatgac gatccagcgg ttgcgggcca aggccgagcg
     2581 cgaactgggc gacaggttcg acattcgcgc cttccacgaa caggtgctcg gctcgggcgc
     2641 cctgcccctg cccgtgctgg aggcgaagat cgaccggtgg attgccggca agcaaggctg
     2701 agcaagatcc gctcctttgt gcccacggca aaaacaggga ggatctgagt gtgaatggcg
     2761 ctaagggcgc ggaatgtccg ttaccgttgc cgcactttac cgatttgccc gtttcgccga
     2821 tccggcagcc ctgcgcgggc cactggatga actggcccgc gcggagggga tcaagggcac
     2881 gctgctgctc gccggcgagg gcatcaatgg caccattgcc gggagtgcag cgggtatcgc
     2941 cgcggtgctt ggccacatcc gtgccctgcc cgattgcgcg gagctggacg tcaagttctc
     3001 ctccgcacct gacatgccgt ttcaccggct caaggtgcgg ctgaagcgcg agatcgtcac
     3061 catgggcgag cccgggatcg atccgcgcgc aaccgtcggc acctatgtcg agtcgcagga
     3121 ctggaac
//
LOCUS       DATNBV010000141         2612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4235551,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000141 DATNBV010000000
VERSION     DATNBV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4235551"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VN627_03405"
     CDS             <1..1094
                     /locus_tag="VN627_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXD02332.1"
                     /translation="PLFGYRSMVAATLFICVVSMMTWLHHFFTMGSGPAVNTAFGIST
                     SVIAVGTGVKIYNWIFTMYRGRVRLGVPMLWSLGFIFTFVIGGMTGVLLAVPPADFLV
                     HNSMFLVAHFHNVIVGGVVFALFAGLDFWFPKAFGFRLHEGWGKASFWFAFLGFWITF
                     APLYVLGLEGMTRRLQHVNQPEWAPMLYLSAAGVGVLAIGVVCQVVMLVVSIRQRERL
                     RDTTGDPWDGRSLEWATPSPPPFFNFAVTPNVEGEEAYWGIKLKAIESQGLGPEPQYR
                     PIEMPVHSPVGFYTAVFAAITGFSLIWKIWWLAGLGLVAAFAGFVIFAWRDIHEFHVP
                     VEQVAEADRARRAAREALLARLAREGALP"
     gene            1091..1786
                     /locus_tag="VN627_03410"
     CDS             1091..1786
                     /locus_tag="VN627_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HXD02333.1"
                     /translation="MTDAPLPAARETIRRVREDPHRLGHRDLHAREAIAGTGHGATGP
                     AHKRVLVGYGFWIFLLSDIVMFACFFAAFAVQRGATAGGPALREIIDVHRVAWETAAL
                     LLSSYTCGLSFAATNARKLVATQAFLLITGLLGLVFVGLELSEFIDLAGRGFVPQRSG
                     MLTAFFGLVGLHGLHVTLAGLWLLTMMAQVQVKGFRPEIVHRLICFNLFWHALDIVWI
                     GIFTIVYLLGAAT"
     gene            1783..2193
                     /gene="cyoD"
                     /locus_tag="VN627_03415"
     CDS             1783..2193
                     /gene="cyoD"
                     /locus_tag="VN627_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HXD02334.1"
                     /translation="MSEPDDALSSGALDAREDHAPGDEPMEDAGHWVRYANLGLGFSV
                     ILTVAAFLIPEADILYAPAIPAALLVLAIAQMGVHLVFFLHITTGPDNTNNVLALAFG
                     LLVVFLIVAGSLWIMSHLSHNMMPPAQAVMRMPL"
     gene            2303..>2612
                     /locus_tag="VN627_03420"
     CDS             2303..>2612
                     /locus_tag="VN627_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02335.1"
                     /translation="MPKYALIAVTSPAEGQDTAFRDWYENVHIPELLSVPGMTGSRRF
                     RIKSSNLGDAGRDQFIAIHEVDTDDLKAVLNGIGQTGKPPACLDPAASRTIITEEIFS
                     S"
BASE COUNT          379 a          917 c          811 g          505 t
ORIGIN      
        1 agccgctgtt cggctaccgc tcgatggtcg ccgcgaccct gttcatctgc gtcgtgtcga
       61 tgatgacctg gctgcaccac ttcttcacga tggggtccgg cccggcggtg aacaccgcct
      121 tcggcatttc gacgagcgtc atcgcggtgg gcaccggggt gaagatctac aactggatct
      181 tcaccatgta ccgcggtcgc gtacgcctgg gcgtgccgat gctgtggtcg ctgggcttca
      241 tcttcacctt cgtgatcggc ggcatgaccg gggtcctgct cgccgtcccg ccggccgatt
      301 tcctcgtcca caactcgatg ttcctcgtcg cccacttcca caacgtgatc gtgggcggag
      361 tggtcttcgc gctgttcgcc gggctcgatt tctggtttcc caaggccttc gggttccgcc
      421 ttcacgaggg ctggggcaag gcctcgttct ggtttgcgtt cctcgggttc tggatcactt
      481 tcgcgcccct ctacgtgctc ggcttggagg gcatgacaag gcgcctccag cacgtgaacc
      541 agcccgagtg ggccccgatg ctctatcttt ccgcggcggg cgtcggcgtg ctggcgatcg
      601 gcgtggtctg ccaggtcgtc atgctggtcg tctccatccg ccagcgcgag cgtttgcgcg
      661 atacgaccgg ggatccctgg gacggccgca gcctcgaatg ggccaccccc tccccgccgc
      721 cgttcttcaa cttcgccgtc accccgaatg tcgagggcga ggaagcctat tgggggatca
      781 agctcaaggc gatcgaaagc caggggctgg ggccggagcc gcagtaccgc ccgatcgaga
      841 tgccggtcca ttccccggtc ggattctaca ccgccgtctt cgccgcgata accgggttct
      901 cgctgatctg gaagatctgg tggctggcag gcctggggct tgtcgccgcc ttcgccggct
      961 tcgtcatatt cgcctggcgc gacattcacg aattccatgt gccggtagag caggtggccg
     1021 aggccgaccg ggcgcggcgg gccgcgcgcg aggccttgct ggcgcggctc gcgcgcgagg
     1081 gggccctgcc gtgaccgacg ccccgcttcc cgcggcgcgc gagacgatcc gccgggttcg
     1141 cgaagatccc catcgcctcg gccaccgcga tctgcatgcc cgcgaggcca tcgccggcac
     1201 ggggcacggc gcaaccggcc cggcccacaa gcgggtgctc gtcggctacg gtttctggat
     1261 attcctcctc agcgacatcg tcatgttcgc ctgcttcttc gcggccttcg cggtccagcg
     1321 cggggcgacc gcgggcgggc cggcgctgcg cgagatcatc gacgtgcacc gcgtcgcctg
     1381 ggaaaccgcc gcgctgctgc tgtcgagcta cacctgcggc ctgtccttcg cggcgaccaa
     1441 cgcgcgcaag ctggtcgcca cccaggcgtt cctgctgatc accggcctgc tcggcctcgt
     1501 cttcgtcggg ctcgagctta gcgaattcat cgacctcgcc gggcgcggct tcgtgcccca
     1561 gcgcagcggc atgctgactg ccttcttcgg gctggtcggc ctgcacgggc tccacgtcac
     1621 cctcgccgga ctatggctgc tgacgatgat ggcgcaggtc caggtcaagg gcttccggcc
     1681 cgagatcgtg caccggctga tctgcttcaa cctgttctgg cacgcgctgg acatcgtgtg
     1741 gatcggcatc ttcacgatcg tctacctcct gggagcagcg acatgagcga acccgacgac
     1801 gcgctctctt cgggcgccct ggatgcccgc gaggaccatg ccccgggcga cgaacccatg
     1861 gaggatgccg ggcactgggt gcgctatgcc aacctcggcc tcggcttttc cgtcattctg
     1921 accgtggcag ccttcctcat tcccgaagcc gacatcctct atgctccggc cattccggcg
     1981 gcgctgctgg tcctggccat cgcccagatg ggggtgcacc tcgtgttctt cctccacatc
     2041 accaccgggc cggacaacac caacaacgtc ctggccctcg ccttcgggct gctggtcgta
     2101 ttcctgatcg tcgcgggatc gctctggatc atgagccatc tcagccacaa catgatgccg
     2161 ccggcgcagg cggtgatgcg gatgccgctc tagcagccat tgccggccct gccgatattg
     2221 acaatatttt gtcattacgg cacacccttg cccaaggcgg cacgtcattt gccgactcca
     2281 accgcgacca aggaccctcg ctttgccgaa atacgcattg atcgccgtaa cctccccggc
     2341 agaaggccag gacaccgcat ttcgcgactg gtacgagaac gtccacatcc ccgagctgct
     2401 cagcgtcccc gggatgaccg gaagccggcg cttccggatc aagtcgtcca atctgggcga
     2461 cgccggccgg gaccagttca tcgccatcca cgaggtggat acggatgacc tgaaggcggt
     2521 cctgaacggc atcgggcaga cgggcaagcc gcccgcctgc ctcgatccgg cggccagcag
     2581 aaccatcatc accgaggaaa tcttttcctc cg
//
LOCUS       DATNBV010000142         6491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2310890,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000142 DATNBV010000000
VERSION     DATNBV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310890"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VN627_03425"
     CDS             <1..242
                     /locus_tag="VN627_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXD02336.1"
                     /translation="ARLRLTLQDRPGALFKVMEEFNAHNINIIEIWHQRIFTHLPAKG
                     LITDIECEARDREQLEALVAALRAKGYDVSQVDVN"
     gene            533..1384
                     /locus_tag="VN627_03430"
     CDS             533..1384
                     /locus_tag="VN627_03430"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HXD02337.1"
                     /translation="MTAPVRFPRFFVTSPSPCPYLPGRMERKVFTELKGPHADALNDA
                     LSRIGFRRSQTVAYRPSCIDCAACQSVRVVAEEFRPSPTQRRNLKRNSDLVAQECQPW
                     STTEQFDLLQRYLGARHPGGGMAQMDDVDFADMVEHTPVTSFVVEYREPGDGITPGRL
                     VGACLTDRQGDGLSMVYSFFDPAHEARAGLGNYIILDHIRRAAQMGLPYVYLGYWVEQ
                     SERMQYKIRYRPLERLGREGWERFDDAEQDRLVAAIAGSRGTVLPVADPGTKDGLPVG
                     DADHAFA"
     gene            1293..3590
                     /locus_tag="VN627_03435"
     CDS             1293..3590
                     /locus_tag="VN627_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXD02338.1"
                     /translation="MPDLAALFCPWPIRAPRTACRWATPITPSPDFLQGRRRSLLLAA
                     AALLLAPLPLHAQPADAELKDLIPDSAVANPEGWAGGTPPAAGNGSTAVPAPPSAPAA
                     DAVAPLAELPGLTVPWPETLPPPPALASLPPEPDVAAELAAQRQEQQAARPDAALAGE
                     QVKVGNHLTLIFPQSFKTFPEQAAFMSRFRALSALRKYDTDKDTIAQVAARARADKDL
                     LERLLRIYGYYDAVVTQAVGNPRVPGAGAGETSADAAVRFFISPGERYRFGAIDLGNL
                     AATGADYPHFHDAFPLHQGEPLDNDRIVAARTSLDTDLGENGYAFAKVGDPDLLVDHK
                     RAEGDLTIPTEPGGKYRFGAVVSRNPRFLSSRHLQDIARFKRGQIYQRTDQEDLKRAI
                     LATGLVSSVTVTPREVTPPPPPTPGAPAPTGEVALDVGLVPGPLRTIAGQVGYDTADG
                     FRLEASWEHRNFFPPEGMLRLRGIAGTKEQLVGTTVRWNNWHGRDQVLTLDLYGNTVN
                     RDAYMARTVAFTATFEKLTTLLFQKPWVWSVGLETLATQERESAVSGQTVPRNTFFVA
                     ALPLRGAIDQSDDLLDPTRGWRAALRVSPEISKQKGGPQVTYARIQADASAYFPVSRK
                     IVLAGRTRVGSIPGTAIENIAPSRRFYAGGGGSVRGYGYQEIGPRDTANAPSGGRSLV
                     EFSLEARIGTGLFGGALQVVPFIDAGSVSASTTPTFHDMRYGAGLGLRYKTGFGPVRI
                     DIGTPLNPRPGDKRVTVSVALGQAF"
     gene            3590..>6491
                     /locus_tag="VN627_03440"
     CDS             3590..>6491
                     /locus_tag="VN627_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HXD02339.1"
                     /translation="MDDTAIPAQQSLAEAGTPEEVVVRRRRTPVWLRWIAAGLAGLLA
                     LMVIAALVLESPIGHRFVVDRIARYAPASGLRVEIGRIDGSLMGSATLRDVKFSDPDG
                     LFLRVPVVQLDWRPLHWFTSGLDVRKLILHRGTLYRGPHLKPGDPNAPLLPDFDIRVD
                     QFQLDRLTVSKGIFGEQRRIDLVARADIRKGRVLLTTDGRLGGQDRLYGYLDSEPKRD
                     RFALKVDIAAPKGGFLAALTGARDSLAIKVDGAGRYTEWNGRALVTQPGQTLADLAIG
                     NRAGRYAFSGPIHPDGFLSGIPARAAGPEVAVAGEGTLVSSVLSGQFSARGRGALIDG
                     NGTVDLARNAFRQLRINAALRDPELFGPGVRLENARLAATLDGAFRDVVIDHRLTADR
                     LLVGGVRAERLVQEGPLTRSGERWTFPLNLTAARVVTGNAMFDKRLMGGHARGTIRLA
                     GTRVFSDPLEIGMPGLGGALTLSGDTRSGDYRLTGPVAARDLALANLGTANADLRLDA
                     RFGAVPWLVNADVRGRMAQVSNATLTTLAGTGVRFAGRLAIGQKQPLLLQHFTVDGSK
                     LALRVDGRVLPNGATSIVGSGRHVDYGPFTVQAQMAGDGPHAVLVFAHPLPAAGLRDV
                     RVALAPIPQGFRIETAGQSRLGPFTGTLGLFSPPGGPTRIVIDKLDVWKTSVTGTLVL
                     SGGAARGTLALTGGGVNGTIGLLPRRGGQGFDVALTVENARFGGEQPLAVGLARINAS
                     GTLANGHTTVNGNAYAEGLQTGSLFIGRLAANAALTDGSGRVTASLAGRRGSRFALQL
                     IADVSPDQYRLLASGVYAGERITMPRRAVLTRTADGWTLAPSQVDFAGGRVIASGSIG
                     EATNLDLALSDMPLSLIDIFTADLGLAGKASGTVTWRQRNGGVPTADARLEIHGLSRS
                     GLVLTSRPIDVSLVARLGERDLETRAVIREGADVRGRLQGRIANMPLAGALGD"
BASE COUNT          953 a         2223 c         2277 g         1038 t
ORIGIN      
        1 tggcgcgcct gcgcctgacc ctgcaggacc gcccgggcgc cctgttcaag gtgatggaag
       61 agttcaacgc ccacaacatc aacatcatcg agatctggca ccagcggatc ttcacccacc
      121 tgccggccaa gggcctgatc accgacatcg aatgcgaggc gcgggatcgc gagcagctgg
      181 aggctttggt cgcggcgctg cgggccaagg gctatgatgt gagccaggtg gacgtgaact
      241 gaggctgtga aggtcgctgc gctccggctc acgcaacagc accattttcg actcaccctg
      301 aaccgcgcca tggatgctga aacaagttca gcatgacggt cagtgggatg ttggcacggc
      361 ctctgcagga gaggcgtggt cgggtgatgg tccagtcccg agcaaaagcg catccgtatt
      421 aaccgcgaaa atgatggtta agcctctttc atccttgccg cggcgtcggc atattgcagt
      481 tacggcgcga acgggtcgcg cgggatggat gggtgaaagg cgccaaggcc aagtgactgc
      541 acctgtccgc tttccccggt tcttcgtcac cagcccgagc ccgtgcccct atctgcccgg
      601 gcggatggag cggaaagtct ttaccgagct caagggcccc catgccgatg cgctgaacga
      661 tgcgctgagc cggatcgggt tccgtcggag ccagaccgtg gcctaccggc cctcgtgcat
      721 cgactgcgcg gcctgccagt cggtccgcgt cgtggcggag gagttccgcc cgtcgccgac
      781 ccagcggcgc aacctcaagc gcaattccga tctggtcgca caggaatgcc aaccctggtc
      841 caccaccgag cagttcgatc tgctccagcg ctacctcggc gcgcgccatc ccggcggggg
      901 catggcgcag atggacgatg tcgatttcgc cgacatggtg gaacataccc cggtcacaag
      961 ctttgtggtg gaatatcggg agccgggtga cgggatcacg ccgggccgcc ttgtcggtgc
     1021 ctgtctgacg gaccggcagg gcgatggcct gtcgatggtc tacagcttct tcgaccccgc
     1081 tcatgaggcg cgcgcagggc ttggcaacta catcatcctc gaccacatcc gccgcgctgc
     1141 ccagatggga ctgccctacg tctatctcgg atactgggtc gaacagtcag agcgcatgca
     1201 gtacaagatc cggtaccggc cgctcgaacg gctcgggcgc gagggctggg aacgctttga
     1261 cgatgccgag caggaccgtc tcgtcgccgc cattgccgga tctcgcggca ctgttttgcc
     1321 cgtggccgat ccgggcacca aggacggcct gccggtgggc gacgccgatc acgccttcgc
     1381 ctgatttcct acagggccgc cgtcgctccc tgctgctggc cgcggcggcg ctgctgctgg
     1441 cgcccttgcc gctccacgcc cagccggccg atgccgagct caaggacctt atcccggatt
     1501 ccgccgtggc caatccggaa gggtgggccg ggggaacgcc gcccgcggcc ggaaacggat
     1561 cgaccgcggt tccggccccc ccgtcggccc ctgccgccga cgccgtggcg ccgctggccg
     1621 agctgccggg gctgaccgtg ccctggcccg aaaccctgcc gccgccgccg gcgctcgcct
     1681 cgttgccgcc ggagccggac gtggccgccg agctggcggc gcagcggcag gagcagcagg
     1741 ccgctcgtcc cgatgcggcg ctggcgggcg aacaggtcaa ggtcggcaat catctgacgc
     1801 tgattttccc gcagagcttc aagaccttcc ccgagcaggc cgccttcatg agccggttcc
     1861 gcgcgctttc ggcgctgcgc aagtacgata ccgacaagga caccattgcc caggttgccg
     1921 cgcgggcccg ggccgacaag gacctgctcg agcggctgct gcggatctat ggctattacg
     1981 atgccgtggt cacccaggcc gtgggcaatc cgcgggtgcc gggagccggg gcgggggaga
     2041 ccagcgccga tgcggcagtg cgcttcttca tctcacctgg cgagcgctat cgcttcggcg
     2101 cgatcgacct cggcaacctg gcggcgaccg gggcagacta tccccacttc cacgacgcat
     2161 tcccgctcca ccagggcgag ccgctcgaca acgaccggat cgtggctgcc cggaccagcc
     2221 tcgacaccga ccttggcgaa aacggctatg ccttcgccaa ggttggcgac ccggacctgc
     2281 tggtcgacca caagcgggcc gagggtgacc tgaccatccc gaccgagccg ggcggcaagt
     2341 accgcttcgg cgcggtggtc agcaggaacc cgcggttcct gtcctcgcgt cacctgcagg
     2401 acatcgcccg gttcaagcgc ggccagatct accagcggac ggaccaggaa gacctgaagc
     2461 gcgcgatcct ggccacgggg ctcgtttcca gcgtgaccgt gaccccgcgc gaggtgacgc
     2521 cgccgccccc accaaccccg ggcgcaccgg cgccgacggg cgaagtggcg ctggatgtgg
     2581 ggctggtgcc gggacccttg cggaccatcg ccgggcaggt gggttatgac acggccgacg
     2641 ggttccggct ggaagccagc tgggagcacc gcaacttctt cccgcccgag ggcatgctgc
     2701 ggctgcgcgg aatcgcgggg acgaaggagc agctggtcgg cacgaccgtg cgctggaaca
     2761 actggcacgg gcgcgaccag gtgctcacgc tcgatctcta cggcaacacg gtcaaccgcg
     2821 atgcctacat ggcccgcacg gtcgccttca ccgccacgtt cgagaagctg acgacgctgc
     2881 tgttccagaa gccgtgggtc tggtcggtcg gcctcgagac gctggcgacg caggagcgag
     2941 agagcgcggt ttccgggcag acagtcccgc gcaatacctt tttcgtcgcc gccctgccgc
     3001 tgcgtggtgc gatcgaccag tcggacgacc tgctcgaccc gacgcgcggc tggcgcgcgg
     3061 ccctgcgggt ttcgccggag atttcgaagc agaagggcgg gccgcaggtt acctatgccc
     3121 gcatccaggc cgatgcctcg gcctatttcc ccgtgagcag gaagatcgtc ctcgccgggc
     3181 ggacccgcgt cggctcgatc cccgggacgg ctatcgagaa tatcgcccca tcgcgccggt
     3241 tctatgccgg cggcggcggt tcggtgcgcg gctacggtta ccaggaaatc ggtccgcgcg
     3301 acacggccaa tgcgccctct ggcgggcgct cgctggtgga gttcagcctt gaggcgcgga
     3361 tcggcacggg cctgttcggc ggggcgctgc aggtggtgcc gttcatcgat gcaggctcgg
     3421 tctcggcttc gaccacgccg accttccatg acatgcgcta cggcgcgggg ctgggcctgc
     3481 gctacaagac cggattcggc ccggtccgca tcgatatcgg cacgccgctc aatccccggc
     3541 ccggcgacaa gcgggtgacg gtcagcgtcg cgcttgggca ggcgttctga tggacgatac
     3601 cgcgatcccg gcgcagcaga gcctggcgga ggcagggaca ccggaggagg tggtggtccg
     3661 ccgtcgccgc actccggtgt ggctgcgctg gatcgccgcc gggctggcgg ggctgctggc
     3721 gctgatggtg attgcggcgc tcgtgctgga gagcccgatc ggccaccgct tcgtggtcga
     3781 ccggatcgcg cgctatgcgc cggcctcggg cctcagggtc gagatcgggc ggatcgatgg
     3841 atcgctgatg ggctcggcaa ccctgcgtga cgtgaagttt tccgatcccg acgggctgtt
     3901 cctgcgggtg ccggtggtcc agctcgactg gcggccgctg cactggttca ccagcgggct
     3961 cgacgtgcgc aagctgatcc tgcatcgcgg cacgctctat cgcgggccgc acctgaagcc
     4021 cggcgatccc aatgcgccgc tgctgcccga tttcgacatc cgggtggacc agttccagct
     4081 tgatcggctg actgtcagca agggcatctt cggcgagcag cggcggatcg atcttgtcgc
     4141 ccgcgccgat atccgcaagg gccgggtgct gctgaccacc gatgggcgcc ttggcgggca
     4201 ggaccggctc tacggctatc tcgacagcga gcccaagcgc gaccggtttg ccctcaaggt
     4261 cgatatcgca gcgcccaagg gcgggttcct ggccgcgctg accggggcca gggatagcct
     4321 cgcgatcaag gtcgacgggg cgggtcgtta taccgagtgg aacgggcggg cgctggttac
     4381 ccagcccggc cagacccttg ccgatcttgc catcggcaac cgcgccgggc gctatgcgtt
     4441 cagcggcccg atccacccag atggctttct ttccggcatc cccgcgcggg ccgccgggcc
     4501 ggaggtggcc gtggcgggcg aggggacgct ggtgtcctcg gtactttcgg ggcagttttc
     4561 cgcgcgcgga cgaggcgctc ttatcgatgg caacggcacg gtggacctgg cccgcaatgc
     4621 cttccgccag ttgcggatca acgccgcgtt gcgcgatccc gagctgttcg gccccggcgt
     4681 gcggctggag aatgcccggc ttgccgcaac gcttgacggc gccttccgcg atgtggtgat
     4741 cgaccaccgg ctgaccgccg accggctgct cgtcggcggg gtgcgggccg aacggctggt
     4801 gcaggaaggg ccactgacca gatcgggcga gcgctggacc tttccgctca acctgaccgc
     4861 cgcccgcgtc gtcaccggca atgccatgtt cgacaaacgg ctgatgggcg gccacgcgcg
     4921 cgggaccatc cggcttgccg ggacccgcgt gttctccgat ccgctggaga tcggcatgcc
     4981 cggccttggc ggggcactga ccctttcggg tgatacccgc agcggcgact atcgcctgac
     5041 cggcccggtg gccgcgcgcg atctggcgct ggcgaacctg ggcacggcca atgccgacct
     5101 gcggctggat gcaaggttcg gggcagtgcc ctggctggtc aatgccgatg tgcgcgggcg
     5161 catggcgcag gtcagcaacg ccacgctgac cacgctggcg ggcaccggcg tgcgctttgc
     5221 cgggcgcttg gccatcggcc agaagcagcc gctcctgctt cagcacttca ccgtcgacgg
     5281 cagcaagctg gcgctgcggg tcgacgggcg ggtcttgccc aatggagcga ccagcatcgt
     5341 cggatcgggc cgccatgtgg actatggccc cttcacggtt caggcgcaga tggcggggga
     5401 tgggccccat gccgtgctgg tcttcgccca tccgcttccg gctgccgggc tgagggacgt
     5461 gcgggtggcg ctcgcgccga tcccgcaggg cttccgcatc gagacggcgg gccaatcgcg
     5521 gctcgggccg tttaccggca cgctcggcct gttctccccg ccgggcgggc cgacgcgcat
     5581 cgtcatcgac aagctcgacg tgtggaagac ttcggtgacg gggacgctgg tcctgtccgg
     5641 cggggcggcg cggggcacgc tggcgctgac gggcggcgga gttaacggga cgatcggcct
     5701 tctgccgcgc cgcggcggac aaggctttga cgtggcgctg accgtggaga atgcccgctt
     5761 cgggggcgag cagccgctcg ccgtgggcct ggcccggatc aatgcctcgg gcacccttgc
     5821 caatggccac accaccgtca acggcaacgc ctatgcggaa gggctgcaga cgggcagcct
     5881 gttcatcggc cgccttgccg ccaatgccgc gctgaccgac ggcagcggcc gggtcacggc
     5941 gagcctcgcc ggtcggcgcg ggagccgctt cgcgctccag ctgatcgccg acgtttcgcc
     6001 cgaccagtac cgcctgctgg cgagcggcgt ctatgcgggc gagcgcatca ccatgccgcg
     6061 ccgggccgtg ctcacccgga ctgcggacgg ctggaccctc gccccgagcc aggtggactt
     6121 cgccgggggc cgggtgatcg cttcgggcag catcggcgag gcgaccaacc ttgacctggc
     6181 gctgtcggac atgccgctgt cgctgatcga catcttcacc gccgacctgg gcctggccgg
     6241 caaggcttcc ggcacggtca cctggcgcca gcgcaacggt ggcgtgccga ccgccgatgc
     6301 ccggctggaa atccacgggc tgtcgcggtc cggcctggtg ctgacctcgc ggccgatcga
     6361 cgtttcgctg gtggcgcggc ttggcgagcg cgacctggaa acccgcgctg tgatccgcga
     6421 gggcgccgac gtgcgcggcc ggttgcaggg gcggatcgcc aacatgccgc tcgccggcgc
     6481 gctgggtgac c
//
LOCUS       DATNBV010000143         3348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3592493,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000143 DATNBV010000000
VERSION     DATNBV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3592493"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1489)
                     /locus_tag="VN627_03445"
     CDS             complement(215..1489)
                     /locus_tag="VN627_03445"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXD02340.1"
                     /translation="MPETGKDLIAQLPLRERDPANPGFMALDNIRVLDFTRALAGPQA
                     TQVLGDFGADIIKVEMPGKGDDSRMSLPQHRIGGESAFFLALNRNKRSIAVDINSDAG
                     REIILDLLETCDVLVENFTSRVMRRIGLDYASLKERFPGLIYCSVSAYGRTGSLSEAA
                     GFDTPVSAEAGLCRLNSRPGEAPTIGGTPWTDLTTGLNAAIAILIALQARSRTGMGQM
                     VEAAMYDTALSNLTFRGYECLVSGEEPRAIPVQALRGIPRGQFATQDGVLVITAGGDK
                     MFRAFATQVIDKPEWAEDPRLATDNGRAGHAEELLGAINAILQGQTSAYWSQRCKAAG
                     VPCGIMKDVGEALLSPETAEREMVYRVPHPTAGAVPVIANPARLWGTPQETAAAPPLL
                     GQHTREILAELAGYDEALVSALIEQGVIGTLG"
     gene            complement(1482..2255)
                     /locus_tag="VN627_03450"
     CDS             complement(1482..2255)
                     /locus_tag="VN627_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD02341.1"
                     /translation="MTGGLDEGSAVLVTGATGGIGQEIAAQAAAAGATVGVHGSRQES
                     VDAAMVKLRSRVPGGQFVDCAADYREPGAIAALVESFVAATGRLDGVVDCALGGPGGR
                     ITGAFTGTDPADFAPHAAGALGNVETLCFAALPHLRRQGGSFVAFASDSGRFAAPRQA
                     LVGATMAGIIGFVRNIGLELARDGIRVNCVTPSFVAETPIFERFSQAAEGRAQAAANR
                     AGLGLPTPADVAPLVLFLLGPGAARITGQVISVNGGLNA"
     gene            complement(2586..>3348)
                     /locus_tag="VN627_03455"
     CDS             complement(2586..>3348)
                     /locus_tag="VN627_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXD02342.1"
                     /translation="DPNSPTYDPQAIPFYKPEKVDDVELGIKADWTAGSIRGRTNIAV
                     FQSNFKDLQRATTYLNQVTHTASSQVGNVAKERSRGFEFETSVKWNDLLETSLSYAYL
                     DAKYTKYDGQTLRIPDNVLVENITSPVSASPKHKINLLTTFNLPVPERVGQVSLTANV
                     SYQSSMVLSDNSISDPIPEVQPAYTLVDLRADWQNVMGSPVDIGFFVKNVANKVYAIG
                     SAHVINSRLGVTGIQYAEPRTFGVRVRARFGASAQ"
BASE COUNT          546 a         1126 c         1099 g          577 t
ORIGIN      
        1 gcagggcata ttcctcggcg agttgtggta cctcgaggaa ctcgccaact ggctgcaggc
       61 gcacgaacgc tcgtcgttcc tgttgaccgc gccgcccctg cgcctgccgg gcagcgtcgg
      121 atctccgctt accgggattg ccacagtgtg aagcggaagg cccttcgccc acgcggaggg
      181 ccttcctgcc cctccaaagg ctggtccggt atgcttaccc gagcgtgccg atgacacctt
      241 gctcgatcag ggcggagaca agggcttcgt catagccggc cagttcggcc aggatttcgc
      301 gggtgtgctg ccccagcagc ggcggcgccg ccgcggtctc ctgcggcgtg ccccacaggc
      361 gggccgggtt ggcgatgacc ggcactgcac cggccgtagg gtgcggcacg cggtagacca
      421 tctcccgctc cgccgtttcc ggcgacagca aggcctcgcc cacatccttc atgatcccgc
      481 atggcacccc cgcagccttg cagcgctgcg accaataggc gcttgtctgc ccttgcagga
      541 tggcgttgat cgcgcccagc agttcctcgg catggccagc gcggccattg tcggtcgcca
      601 ggcgcggatc ttcggcccat tccggtttgt cgattacctg ggtggcgaag gcccggaaca
      661 tcttgtcccc gccggccgtg atcaccagca cgccgtcctg cgttgcgaac tgcccgcgcg
      721 ggatgccgcg caaggcctgc accgggatgg ctcgcggctc ctcgcccgat accaggcact
      781 cgtacccgcg gaaggtcagg ttcgacagcg cggtgtcata catcgccgct tcgaccatct
      841 gccccatgcc cgtgcggctg cgggcttgca gggcgatgag gatcgcaatg gcggcattga
      901 ggcccgtggt cagatcggtc cacggcgttc cgccgatggt cggcgcttcc cccggccgcg
      961 agttcagccg gcacaggccg gcctcggccg aaaccggcgt atcgaacccg gccgcttccg
     1021 aaaggctgcc ggtgcgcccg taagccgaaa cggagcagta gatcagcccg gggaagcgct
     1081 ccttcaggct ggcgtagtcc agcccgatcc ggcgcatgac ccggctggtg aagttctcca
     1141 ccagaacgtc gcaggtctcc agcaggtcga gaatgatctc gcgccccgca tcgctgttga
     1201 tgtccaccgc gatcgaccgc ttgttgcggt taagcgcgag gaaaaacgcg gattcgcccc
     1261 cgatgcggtg ctgcggcagc gacatgcgcg aatcgtcgcc cttgccgggc atttccacct
     1321 tgatgatgtc cgcgccgaag tcgcccagca cctgggtcgc ttgcggtccg gcgagggcgc
     1381 gcgtgaaatc gagcacccgg atgttgtcga gcgccatgaa gcccggattg gcggggtcgc
     1441 gttcgcgcag cggcagctgg gcgatcaggt ccttgccggt ctcaggcatt gagccctccg
     1501 ttgacgctga tcacctggcc ggtgatgcgc gcggcgcccg gccccagcag aaacagcacc
     1561 agcggtgcga cgtccgccgg ggtcggcagg cccagcccgg cgcgattggc cgccgcctgc
     1621 gcgcgtccct cggcggcttg cgaaaaacgc tcgaagatcg gggtctcggc cacgaagctt
     1681 ggtgtcacgc agttgacccg gatgccatcg cgcgcaagct ccaggccgat gttgcgcacg
     1741 aagccgatga tgcccgccat cgtcgccccg acgagcgcct ggcgcggcgc ggcgaagcgg
     1801 ccggaatccg atgcgaaggc cacgaacgat ccgccctgcc ggcgcagatg cggcagcgcg
     1861 gcgaagcaca gcgtttccac gttgccaagg gcaccggcgg catggggcgc gaagtcggcg
     1921 gggtcggtgc cggtgaacgc cccggtgatc cggccgccgg gaccgcccaa tgcgcagtca
     1981 accacgccgt cgagccggcc cgtggctgca acgaagctct cgacgagggc ggcgatcgct
     2041 cccggctcgc ggtaatcagc agcgcagtca acgaactgcc cccccggcac gcgcgagcgc
     2101 agcttcacca tcgcggcatc gaccgactct tgccgcgaac cgtgcacgcc cacagtggcg
     2161 ccggcggcgg cagcctgtgc ggcgatttcc tgcccgatcc cgcccgtcgc tccggtgacc
     2221 aggacagcac tgccttcgtc caatccgccc gtcatgccgg ctgagctccc aatcttgtgg
     2281 cgcgttgtcg cccatcttta tccagcggca tacagctcgc caaattcagc cgatatatgc
     2341 tgtgggaatg gcgcaattgg ccgcccctta atggccccat cccgctgacg cggcggcgca
     2401 agggttggca gcggtgatct ggtggtcgtg ctggctcaat gaaccaacgg agatgacgcc
     2461 cgctccggca tggctgccgc tctagcggga caggccggcc ctgtaactcg atcccgcaag
     2521 ggccgcgcgg gaatgcacct tggcatggcc ggcaagtgct gccggccatg ccatcggtgt
     2581 ctgctttact gggcagaagc cccgaagcgc gcgcgaacgc gcacgccgaa ggtccgtggt
     2641 tcggcatact ggataccggt gacgccgagc cgtgaattga tcacgtgagc cgaaccgatc
     2701 gcatagacct tgttcgccac gttcttcacg aagaacccga tgtcgaccgg cgatcccatc
     2761 acgttctgcc agtcggcccg caggtcgacc agggtatagg ccggctggac ctccggaatc
     2821 ggatcggaga tgctgttgtc cgacaggacc atcgacgact gataggacac gttcgccgtc
     2881 aggctgacct ggccaacgcg ctccggaacc ggaaggttga aggtggtcag caggttgatc
     2941 ttgtgcttcg gcgaggccga gaccggcgag gtgatgtttt caactagaac gttgtcggga
     3001 atacgaagag tttggccgtc atatttggtg tacttcgcgt caaggtaggc atagctgagc
     3061 gaggtttcga gcaggtcgtt ccacttgacc gaggtctcga actcgaaacc gcgcgaacgc
     3121 tccttcgcca cattgcccac ctgcgacgat gccgtgtgcg tgacttggtt cagataggtc
     3181 gtagcgcgct ggaggtcctt gaagttcgac tggaacacgg cgatgttcgt ccgcccgcgg
     3241 atcgagccgg cggtccagtc agccttgatg cccaactcca cgtcgtccac cttctccggc
     3301 ttatagaagg ggattgcctg cggatcgtac gtcggggagt tgggatcc
//
LOCUS       DATNBV010000144         3985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1356399,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000144 DATNBV010000000
VERSION     DATNBV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356399"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /gene="malQ"
                     /locus_tag="VN627_03460"
     CDS             <1..1392
                     /gene="malQ"
                     /locus_tag="VN627_03460"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HXD02343.1"
                     /translation="DFSPYSPSSRIFLNPALADPALLGLPPLPSRSGADLIDWPSALP
                     ERLAVLRASFAGLGEEQRARISQANAREGEGLARHALHDALYCHFRAVGLADWRSWPL
                     PFRDPTSTAVREFAAANPDEIAFHAFAQWLSREGLAAVQGRARGAGMALGLLADLAVG
                     VRPGGSDCWAMPDAMLTGLTIGAPPDPLGPAGQNWGITSYSPEGLRASGYAPFLAMIR
                     AALRSAGGLRIDHAFGLARLWVIPEGGTCLDGAYLTYPFLDLVRLVTLEAHRASAMII
                     AEDLGTAPPGFTPAVTQRNMLGMRVLWFERAADHGFIGAQDYPDGSVAMTGTHDTPTV
                     AGWWRGRDLDWAERLGRLPPEVDRRKAEEIRDWDRGLLWSTIGRGERPAPDDPEPVVA
                     AALEHVARSPAAVAIVPVEDLLADPEQPNLPGTTSEHPNWRRRLSAPLGDLLADPSVA
                     SRLAGLARCGRAG"
     gene            1491..2303
                     /locus_tag="VN627_03465"
     CDS             1491..2303
                     /locus_tag="VN627_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD02344.1"
                     /translation="MDMQLQGKTALVTGSSSGIGIGIAMTLAAEGVAVVVHGRDEGRA
                     RATADKIVGMGGTAAVAVGDLDSDVAADHVAAQALAAFGQIDILVNNAGGREDGAPLA
                     FFDIAPEQWNRTYNSNVTSAVRMIHRLVPAMRERGWGRVIQVSSVAASSSSGGVAEYA
                     AAKAGLLNLSLALSKVLANTGVTVNTISPGMILTGALEGWLDGIAAQRGFGADRDKAA
                     RWVLENSLKQTVDRLGTPDDIGYAASFLCSPRGAFVNGANIRVCGGASPSVN"
     gene            2453..3793
                     /locus_tag="VN627_03470"
     CDS             2453..3793
                     /locus_tag="VN627_03470"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXD02345.1"
                     /translation="MGPALLGLCLISLSLPARAELPSPDPAPLAVQAQLSLPANADLR
                     SFYERRGDRPLWTAGGVLSPAALDALALIRTADLDGVSPDEIGAPALDLAIGQAQSSP
                     SPGALAAAELALSRSFSAYVRRTLEAPEGGMLYEHVSLEPQVPTEARALEAAAGAPSL
                     DEYVHRMAWLHPLYASLRRAYIAAGEQDDATRSAVIANLARIRALPAVPPGGRYVLVN
                     AAAARLWMYEGDRPVDSMKVVVGKVDHQTPIMSGYIRYAIINPYWNVPADFARDKIAG
                     QVLAHGTAYLRRQGYQVLSNWTPDAVVADPATVNWKAVAQGNELLRVRQLPGPANSMG
                     QVKFEFPNQLGIYLHDTPQTQLMQEAARQFSSGCVRLEDAPRLGRWLFGGEMPQGSAT
                     EERFDLPQLVPVYITYLTVAPENGTLAMIGDPYGRDRSAQPALAIEAVGKVASR"
BASE COUNT          600 a         1326 c         1384 g          675 t
ORIGIN      
        1 gatttcagcc cctattcccc ctcgagccgg atcttcctca atccggcact ggcagatccg
       61 gcactgctcg gcctgccacc gctgcccagc cggtccggcg cggatctgat tgactggccc
      121 tcggccctgc cggagcggct ggcggtcttg cgcgcgagtt ttgctggcct cggcgaggaa
      181 cagcgcgccc ggatttcgca ggccaatgcg cgggaaggcg aggggcttgc ccgtcacgcg
      241 ctgcacgatg cgctgtattg ccacttccgg gctgtgggcc tggcggattg gcgctcatgg
      301 cccctgccat tccgcgatcc gaccagcacc gccgtccgcg agttcgccgc cgccaacccc
      361 gacgagatcg ccttccatgc ctttgcccaa tggctcagcc gcgaagggct ggcggccgtg
      421 cagggccgtg cgcgcggcgc aggcatggca ttgggcctgt tggctgatct cgcggtcggg
      481 gttcgcccgg gcgggagcga ctgctgggcc atgccggatg ccatgctgac gggcctcacc
      541 atcggcgcgc cgcccgatcc gttggggccg gccgggcaga actggggcat caccagttat
      601 tcgcccgaag gcttgcgcgc cagcggctat gcgccgttcc tcgcgatgat ccgcgccgcc
      661 ctgcgctcgg ccggggggct gcggatcgac catgccttcg gactggcgcg gctctgggtc
      721 attcccgagg gcggcacatg tctcgacggg gcttacctta cctacccgtt cctcgatctg
      781 gtgcggctgg tcacgctgga agcacaccgc gcttcggcga tgatcattgc cgaggacctg
      841 ggaacggcac ccccgggttt cacccctgcg gttacccagc ggaacatgct cggcatgcgg
      901 gtgctgtggt tcgagcgggc tgccgatcac ggcttcatcg gggcgcagga ctatccggat
      961 ggcagcgtcg ccatgaccgg gacccacgat acgccgaccg tggctggttg gtggcgtggc
     1021 cgcgatctcg actgggccga gcggctcggc cgcctgccgc ccgaagtgga tcgccgcaag
     1081 gccgaggaaa tccgcgactg ggaccgcggg ctgctgtggt cgacgatcgg ccggggagag
     1141 cggccggcac cggacgaccc ggaaccggtg gtagcggctg cgctggagca tgttgcccgc
     1201 tcgcccgcgg ccgtggcgat cgtcccggtc gaggacctgc tcgccgatcc cgaacagccc
     1261 aacctgccag gcacaaccag cgaacacccg aactggcgcc gccgactgag cgcgccgctt
     1321 ggcgatctcc tggccgatcc gtccgtggct tcgcggcttg caggacttgc gcggtgcggc
     1381 agggctggct gaccctgtcc ccgaagattc acgccgagaa atatcgcttg ctcttcccgt
     1441 caaaccttgg cccatttttg accacatcac gagaacggaa ggatcgggcg atggatatgc
     1501 aattgcaggg caagactgcc ctggtcaccg ggagcagctc gggcatcggg atcggcatcg
     1561 ccatgacgct tgctgccgaa ggtgtcgccg tcgtggttca cgggcgtgac gaggggcggg
     1621 cccgcgccac ggcagacaag atcgtcggca tgggcggtac agccgcagtt gccgtaggcg
     1681 atctcgacag cgatgtcgcg gccgaccatg tcgccgccca ggcgctggcg gccttcggtc
     1741 agatcgatat cctcgtgaac aatgccgggg ggcgcgagga tggtgccccc ctggccttct
     1801 ttgacatcgc gcccgagcag tggaaccgca cctacaacag caatgtcacc tcggcggtcc
     1861 gcatgatcca ccggctggtg ccggccatgc gcgagcgggg gtggggccgg gtgatccagg
     1921 tttcgagcgt tgccgcttca tcctcatcgg gcggagtggc ggaatatgcc gcggccaagg
     1981 ccgggctgct caacctttcg ctggccctgt cgaaggtgct cgccaatacc ggcgtcacgg
     2041 ttaacacgat ctcgcccggg atgatcctga cgggggcgct ggaaggttgg ctcgatggca
     2101 tcgccgccca gcgcggtttc ggtgcggacc gggacaaggc ggcccgttgg gtgctggaaa
     2161 acagcctcaa gcagacggtg gaccggctcg gcaccccgga cgacatcggt tatgcggcga
     2221 gcttcctgtg cagcccgcgc ggggccttcg tcaacggcgc caatatccgg gtctgcggag
     2281 gagcttcgcc ctcggtaaat tgaacggggc gccggagggg ggggtcttag ggcttgcacg
     2341 catccagtaa cgtgagtgcc caatggttca cgtcggaact gggtgtcgtt tacccggttc
     2401 tttcaccatg tcgacaacaa ctgaaacatt cacgcgaccg cgtcggaaaa tcatggggcc
     2461 ggcgctgctg ggcctgtgcc tgatttcgct ttcgctgcct gcccgcgccg agctgccaag
     2521 ccctgacccg gcgccccttg ccgtgcaggc acagctctcc ctgcccgcga atgccgacct
     2581 gcgatcattc tatgagaggc gcggggaccg gccattgtgg acggcgggtg gagtgctgtc
     2641 gccggcggcg ctggacgccc ttgcgctcat ccgcaccgcc gatctcgatg gcgtgagccc
     2701 ggacgagatc ggggctccgg cgctggatct ggcgatcggg caggcgcagt ccagtccgtc
     2761 gcccggtgcg ctcgccgccg ccgagctggc cttgtcgcgc agcttttccg cctacgtccg
     2821 ccgcaccctc gaagcgcctg aaggaggaat gctttacgag cacgtctcgt tggaaccgca
     2881 ggtgcctacc gaggcgaggg cgctggaggc tgctgctggg gctccgtcgc tggacgaata
     2941 tgtccaccgc atggcctggt tgcacccgct ctacgcctcg ctgcgccgtg cctatatcgc
     3001 cgcgggcgag caggatgatg cgactcggag cgcagtcatc gccaacctcg cgcggatccg
     3061 cgccctgcct gcggtccccc cgggcgggcg ctatgtgctg gtcaatgccg cggcggcgcg
     3121 gctgtggatg tacgagggcg acaggccggt cgattcaatg aaggtcgtgg tcggcaaggt
     3181 ggatcaccag acgccgatca tgtcgggcta catccgctat gcgatcatca acccctactg
     3241 gaacgtaccg gccgacttcg cccgggacaa gatcgccggg caggtgctgg cccatggcac
     3301 cgcctacttg cgccggcagg gctatcaggt cctttccaat tggacgcccg acgccgtggt
     3361 tgccgatccg gccacggtga actggaaagc ggtcgctcaa ggcaacgagc tgctccgcgt
     3421 gcgccagctg ccgggaccgg ccaattcgat gggccaggtc aagttcgagt ttcccaatca
     3481 gctcggcatc tacctccacg atacgccgca gacccagctg atgcaggagg ccgcgcgcca
     3541 gttcagttcg ggatgcgtgc gcctggaaga tgcgccgcgg ctgggccggt ggctgttcgg
     3601 cggggaaatg ccccagggca gcgccaccga ggagcggttc gacctgcccc agctggtgcc
     3661 ggtctacatc acttatctca ctgttgcccc ggaaaacggg accctcgcga tgatcggcga
     3721 cccctatgga cgcgatcgca gcgcacaacc ggctctggca atcgaggcgg tcgggaaggt
     3781 tgcttcgcgc taagcccggt cgggttcttc agccaaaccg atggacgaaa gaaaaagccg
     3841 ggcgaagcag ctgcttcgcc cggccaattc ttgtgaccct gccgcaggtc agccgcgctc
     3901 gccgcgggtc gggggcggcg ggggaggcgg cgggggcggc ggcggcggag gaggcggcgg
     3961 cggaggcgca gcttccatcg gcggc
//
LOCUS       DATNBV010000145         2350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_729832,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000145 DATNBV010000000
VERSION     DATNBV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729832"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /gene="rplD"
                     /locus_tag="VN627_03475"
     CDS             complement(<1..173)
                     /gene="rplD"
                     /locus_tag="VN627_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HXD02346.1"
                     /translation="MKVKVLNLDGTAGKGEVELSDAVFGLAPRADILHRVVTWQLENR
                     RGIARKARERSDVA"
     gene            complement(177..929)
                     /gene="rplC"
                     /locus_tag="VN627_03480"
     CDS             complement(177..929)
                     /gene="rplC"
                     /locus_tag="VN627_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HXD02347.1"
                     /translation="MRTGVIAKKVGMTRLFQEDGRHVPVTVLSLEDCQVVSVRTAERD
                     GYVALQLGAGQAKQKNVAKPQREHFAKAEVPLKAQVAEFRVADDAVVEVGSTIAASHF
                     VAGQLVDITGQTQGKGFQGAMRRWGFGGMRATHGVSISHRAHGSTGNRQDPGRVFKNK
                     KMAGHMGDRQRTQQNLEVVRTDDERGLIFVKGSVPGSKNAWLLVRDAVKVSRHGEAPY
                     PAGLKQAANSNTPAADTPAPEAAPEAAEGQEG"
     gene            complement(1190..1501)
                     /gene="rpsJ"
                     /locus_tag="VN627_03485"
     CDS             complement(1190..1501)
                     /gene="rpsJ"
                     /locus_tag="VN627_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HXD02348.1"
                     /translation="MEAQNIRIRLKAFDHRVLDQATGEIAETARRTGALIRGPIPLPT
                     RIEKFCVNRGPHIDKKSREQFEVRTYKRLLDIVQPNAATVDALMKLDLAAGVNVEIKL
                     A"
     gene            complement(1633..>2350)
                     /locus_tag="VN627_03490"
     CDS             complement(1633..>2350)
                     /locus_tag="VN627_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HXD02349.1"
                     /translation="SSYGFDGDNIPIIPGSALAALEGRDDNIGKESIYALMKAVDEFI
                     PQPPRPTDKPFLMPVEDVFSISGRGTVVTGRVETGIVKVGEEVEIVGLKDTQKTVVTG
                     VEMFRKLLDQGEAGDNIGALVRGIKREEVERGQVLCKPGSVKPHTEFSAEVYVLSKDE
                     GGRHTPFFANYRPQFYFRTTDVTGEVVLPEGTEMVMPGDNLTLAVKLIAPIAMDEGLR
                     FAIREGGRTVGSGVVSKITK"
BASE COUNT          395 a          748 c          781 g          426 t
ORIGIN      
        1 gcaacatcgg aacgctcgcg cgccttgcgg gcgatgccgc ggcggttttc cagctgccag
       61 gtgacgacgc ggtgcaggat gtcggcgcgc ggcgcgaggc cgaacacggc atccgacagc
      121 tcgacttcgc ccttgccggc agtgccgtcg aggttgagga ccttgacctt cacgacttag
      181 ccctcctggc cttcggccgc ctcgggcgcc gcttcgggag cgggcgtgtc ggcagccggg
      241 gtgttgctgt tagcggcctg cttcagaccg gcggggtacg gcgcttcacc gtggcgcgag
      301 accttgacgg cgtcgcggac gagcaaccag gcgttcttcg agccgggcac cgagcccttg
      361 acgaagatca ggccgcgctc gtcgtcggtg cggacgacct cgaggttctg ctgggtgcgc
      421 tgacggtcgc ccatgtggcc ggccatcttc ttgttcttga agacgcggcc cggatcctgg
      481 cggttaccgg tcgaaccgtg agcacggtgg ctgatcgaca cgccgtgcgt ggcgcgcatg
      541 ccgccgaaac cccagcggcg catggcgccc tggaagccct tgccctgggt ctggccggtg
      601 atgtcgacca gctggcccgc gacgaagtgc gaggcagcga tggtggaacc cacctcgacc
      661 acggcatcgt cggcaacgcg gaattcggca acctgggcct tgagcggcac ttccgccttg
      721 gcgaagtgct cacgctgcgg cttggcaacg ttcttctgct tggcctggcc ggcacccagc
      781 tgcagcgcga cataaccgtc acgctccgcg gtgcggaccg aaaccacctg gcagtcttcc
      841 agcgaaagca cggtaaccgg cacgtgccgt ccgtcttcct ggaaaaggcg ggtcatcccc
      901 accttcttag cgatcacgcc ggtgcgcatg atccattctc ctcacagagg cccacggaga
      961 ccatccccgc gggcgtgttc ggccctattt gtgaagcgtg ccccgtccgg gccgaagtgc
     1021 tgccatttgc ccgaaggctc gcggcagcga gacgggggac gcttgcccgg ctgtgcccga
     1081 aagcggcgcc ggaggtatcc ctatgtccgt gcggtttccc gcatgaccgg ccgaaaccgg
     1141 aagagatttt tggtccgaag accgaaactt gcgtcttgat cgaggccgat caggccaact
     1201 tgatctcgac gttcacgccg gcagccaggt cgagcttcat cagcgcgtcg acggtggcgg
     1261 cattcggctg cacgatatcg agaagccgct tgtaggtgcg gacctcgaac tgctcacgcg
     1321 acttcttgtc gatgtgcggg ccgcggttga cgcagaactt ctcgatacgg gtcgggaggg
     1381 gaatgggccc acggatcagc gcgccggtgc ggcgggcggt ctccgcgatt tcgccggtcg
     1441 cctggtcaag cacgcggtga tcgaatgcct tgaggcgaat gcggatattc tgagcttcca
     1501 tgtccactac cgatgagaaa gagcaaaaac aaaaccgacc gatccgccct cttccatctc
     1561 ggcaaagagc ggagcgatcg ttgaaaaccg gaccggcggg gaagcgaatc ccccgccggt
     1621 gcgcggccta tattacttgg tgatcttgct gacaacccca gaaccgacgg tccggccacc
     1681 ttcgcggata gcgaagcgga gaccttcgtc catggcgatc ggcgcgatca gcttaacggc
     1741 cagggtcagg ttgtcgcccg gcatcaccat ctcggtgccc tcggggagaa cgacctcacc
     1801 ggtgacgtcg gtggtgcgga agtagaactg cgggcggtag ttggcgaaga acggcgtgtg
     1861 acggccacct tcgtccttcg acagcacgta gacttcggcc gagaactcgg tgtgcggctt
     1921 gaccgaaccg ggcttgcaga ggacctggcc gcgctcgact tcctcgcgct tgatgccgcg
     1981 caccagcgcg ccgatgttgt cgccggcttc gccctgatcg agcagcttgc ggaacatttc
     2041 gacgccggtg acaacggtct tctgggtgtc cttgaggccg acgatctcga cttcctcgcc
     2101 aaccttgacg atgccggtct cgacgcggcc ggtgacgacc gtaccacgac ccgagatcga
     2161 gaacacgtct tcgacgggca tcaggaaggg cttatcggtc ggacgcgggg gctgcgggat
     2221 gaattcgtcg acggccttca tcagcgcgta gatcgattcc ttgccgatgt tgtcgtcgcg
     2281 gccttcgaga gcggccagag ccgaacccgg gatgatgggg atgttgtcgc cgtcgaagcc
     2341 gtacgagctg
//
LOCUS       DATNBV010000146         2534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2952590,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000146 DATNBV010000000
VERSION     DATNBV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2952590"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VN627_03495"
     CDS             <1..972
                     /locus_tag="VN627_03495"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HXD02350.1"
                     /translation="YKIYTPFWRALRERLPPAEPLPAPARLPAPANWPASDRLEDWGL
                     LPTAPDWAGGLRAEWCPGERGAGERLTDFAGRAARYEQQRNLPGEEGTSLLSPHLHFG
                     EISPAAVWHAVAGAGGSVGTFLSELGWRDYAQNVILQLPDYASHNARAEFDHLPWRTG
                     PEAEADLRAWQQGRTGYPIIDAGMRQLWHSGWMHNRVRMLAASFLIKHLLIDWREGER
                     WFWDTLVDADYAQNAVNWQWSAGSGVDANMFSRIMAPLGQSAKFDAAGYIRQWVPELA
                     GLSDADIHDPVCRPGGYPARIVDHAFARGRALAALAEAKDAAAADWG"
     gene            complement(1004..1357)
                     /locus_tag="VN627_03500"
     CDS             complement(1004..1357)
                     /locus_tag="VN627_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXD02351.1"
                     /translation="MSQVQTHKRYEVAAQEDRCAPRTRISIPATLRASGGRSFSTIVH
                     DLSLGGFCASAINRMQPGTVCWITLPGLESLQSEVVWWNNSLIGCAFQNLLSPIVHDN
                     ILARWQPETIFRSTL"
     gene            complement(1442..2167)
                     /locus_tag="VN627_03505"
     CDS             complement(1442..2167)
                     /locus_tag="VN627_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD02352.1"
                     /translation="MSQAEKPFAGQVALVTGASKGIGAATALALGKAGAHVILTARDT
                     KGLEAIEEAIHQAGGTATIAPVDLAEPDGIARLATAIRSRWDKLDIMVIAAALLPQLS
                     GVADIDQPSFNKALTVNVLATQSLIAHFDSLLRRSPDPRVIGLTSTVATAPRAYWGAY
                     AATKAAFEQLLDSWAQEVRAVSRIRVAIVNPGATRTAMRARAYPGEDPASVKLPEVVA
                     ERLVSLLGEQFSSPHRETVNQAA"
     gene            complement(2172..>2534)
                     /locus_tag="VN627_03510"
     CDS             complement(2172..>2534)
                     /locus_tag="VN627_03510"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445413.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXD02353.1"
                     /translation="GTTSLKIVQMMRDAGAAEVHMRIASPPTEHSCFYGVDTPERSKL
                     LAARMDVKAMAEFIQADSLAFVSIDGLYRAVGLAGRNAACPQYCDACFSGDYPTRLTD
                     LADRERPAQLTLPVDKVA"
BASE COUNT          407 a          805 c          924 g          398 t
ORIGIN      
        1 tacaagatct acacgccgtt ctggcgggcg ctgcgcgaac gcctgccgcc ggccgaaccg
       61 ctgccggccc cggcgcgctt gccagccccg gcgaactggc ccgcgtccga ccggctggag
      121 gactgggggc tgctccccac cgcgccggac tgggcgggcg gcctgcgagc ggaatggtgc
      181 ccgggcgaac ggggcgcggg cgagcgcctg accgatttcg ccgggcgcgc cgcccgctac
      241 gagcagcagc gcaaccttcc gggggaggag ggtacctcgc tgctgtcccc ccatctccac
      301 ttcggcgaga tttcaccggc ggcggtgtgg cacgcggtgg cgggggcagg gggctcggtc
      361 ggcactttcc tgagcgagct gggctggcgc gactatgccc agaacgtgat cctgcaattg
      421 cccgactacg ccagtcacaa cgcccgggcc gagttcgacc acctgccgtg gcgcaccggc
      481 cccgaagccg aggccgatct gagagcctgg cagcagggcc gcaccggcta tccgatcatc
      541 gacgccggaa tgcgccagct gtggcacagc gggtggatgc acaaccgggt gcggatgctg
      601 gccgcctcgt tcctgatcaa gcacctgctg atcgactggc gcgaggggga gcggtggttc
      661 tgggatacgc tggtcgatgc ggactacgcg caaaacgccg tgaactggca gtggagcgca
      721 gggtccggcg tggatgcgaa catgttctcg cggatcatgg cgccgctcgg ccagtcggcc
      781 aagttcgatg cggcgggcta tatccggcaa tgggtgccgg agctcgccgg gctgtccgat
      841 gcggatattc acgatccggt gtgccggccg ggcggctatc cggcgcggat cgtggatcac
      901 gcgtttgccc ggggcagggc gcttgcggcc ctggccgaag caaaggacgc ggcggctgcg
      961 gactggggat aatccgcaac cgccgcgccc ggccgggtat ctgtcagagt gtcgagcgga
     1021 agatcgtctc gggctgccag cgggccagga tgttgtcgtg cacgatcggc gagagcaggt
     1081 tctggaaggc gcagccgatc aggctgttgt tccaccacac cacttccgac tgcagcgatt
     1141 cgaggccggg cagcgtgatc cagcacaccg tgcccggctg catccggttg atcgccgagg
     1201 cgcagaagcc gccgagcgaa agatcatgga cgatggtgga gaagctgcgg ccccctgacg
     1261 cgcgcagcgt ggccggaatg gaaatgcgcg tgcgcggggc gcagcgatct tcctgcgcgg
     1321 ccacttcata gcgcttatgg gtttgaacct ggctcatcga cttgctccct accccctacg
     1381 caaaaccgcg caggtcggtg aataccgggt aaagaatggg ccaagactgc ttgccggcag
     1441 gttatgcggc ttggttaaca gtttcgcggt gtggtgagct aaactgttca ccaagaaggc
     1501 taaccagcct ctcggcaacc acctccggca gcttgacgga agccggatcc tcacccgggt
     1561 aggcgcgggc gcgcatggcc gtgcgcgtgg cgccagggtt aacgatggcc acgcggatcc
     1621 gggaaacggc gcgaacttcc tgggcccacg aatcgagcag ctgctcgaag gcagccttgg
     1681 tcgcggcata ggccccccaa taggcgcgcg gggcggtggc gacggtggag gtgaggccga
     1741 tcacccgcgg atcggggctg cgccgcagca gcgaatcgaa gtgggcgatc agcgactggg
     1801 tggcgagcac gttgaccgtc agcgccttgt tgaaactggg ctggtcgata tcggccacgc
     1861 ccgagagctg cggcagcagg gccgccgcga tgaccatgat gtcgagcttg tcccaccggc
     1921 tgcggatcgc cgtggccagg cgggcgatgc catcgggctc agccaggtcc accggcgcga
     1981 tcgtggctgt gccgccggcc tggtgaatgg cttcctcgat ggcctcgaga cccttcgtgt
     2041 cgcgcgcggt gaggatcacg tgggcgcccg ccttgcccag cgccagggcc gtcgccgcgc
     2101 caatgccctt gctcgcgccg gtgacgagcg caacctggcc cgcgaagggc ttctccgcct
     2161 ggctcatatg ctcaggcgac cttgtcgacg gggagggtaa gctgggccgg acgctcccgg
     2221 tcagccaggt cggtcaggcg ggtgggatag tcaccgctga agcaggcgtc gcaatattgc
     2281 gggcaggcgg cgttgcggcc agccaggccc acggcgcgat agagcccgtc aatcgagacg
     2341 aacgcgaggc tgtcggcctg gataaattcg gccatggctt tcacatccat ccgcgcggcg
     2401 agcagcttgg agcgttcggg cgtatcgacg ccatagaagc aggaatgctc ggtcggcggg
     2461 gaggcgatgc gcatgtgcac ttcggcggcg ccggcgtcgc gcatcatctg gacgatcttg
     2521 aggctggtgg tgcc
//
LOCUS       DATNBV010000147         3187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_396169,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000147 DATNBV010000000
VERSION     DATNBV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396169"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1795)
                     /locus_tag="VN627_03515"
     CDS             complement(173..1795)
                     /locus_tag="VN627_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HXD02354.1"
                     /translation="MSSQTHGTDVVDKAVEPGAEAVPPMSGPGSWTDPVLARTIPQLI
                     HAAAATYGDKLAFRLAEGEGELREISFRGLDERSAELGRALLARGAGKGSRIGFILTN
                     GPDWVVTYAAIARIGAVPVPISTMLRAAELVRVLRQSDVGGLIVQRRVLGHDMAERLA
                     EALPELAAPGAGDLRLPAAPYLRWICSSGEGLPPGIGTLSDLRARAAEVDAALLAEIE
                     REVHPTDQAIEIYTSGSMAAPKGVRHLHGPIMFRAQWLSGRMVPSMSAQPEFPLVMPM
                     FWVGGLMMGLMMTWVRGVTGLCTEATKINSRHALGSMLTPEDIAEMLKSPPYWGLGMS
                     ETLAAYSWGDEVRAEGWPLAVPLDHWAPGYDIRVVDEDGREVVDGETGEIQVRGYPVT
                     PALHKIERAAYFTPDGFFHTGDLCTRDGARFHFVGRGGDMIKTAGANVSPAEVEDELQ
                     RMDGVHSAYVVGLPDAERGQLVVAALVAREGMTLDLAVIEQEMRRRLSGYKVPRAYVE
                     IAREEVPMMHSNKVYRRELERLLAERLGRAAA"
     gene            complement(2082..>3187)
                     /locus_tag="VN627_03520"
     CDS             complement(2082..>3187)
                     /locus_tag="VN627_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXD02355.1"
                     /translation="KKDDLRPRLTLHDDTSGESARYMLAPGTTLSVEDGQDVQAGDIL
                     ARASREAAKTRDITGGLPRVAELFEARIPKDNAIIAKISGRIEFVRDYKAKRKIAIIP
                     EEGEPIEYLIPKSRVIDVQEGDWVKKGDNLISGSPDPHDILEVLGVEALAEYLVAEIQ
                     EVYRLQGVKINDKHIEVIVRQMLQKVEITDGGDTVLLAGEQVDREEMDEINAKLPEGH
                     APAVGKPVLLGITKASLQTRSFISAASFQETTRVLTQAAVEGKRDSLIGLKENVIVGR
                     LIPAGTGAGMNRLRVAASSRDAALRAQYRKLQESLIAPETAAEEHAAELAQGPEAAIG
                     DDPLAAVEGETHGTDADAGDYLVANEAEDNAGE"
BASE COUNT          482 a         1156 c         1018 g          531 t
ORIGIN      
        1 cgaagcagac ccgccgcctt catggcggcg atagtcgact cggggatatc gcgccgcacc
       61 tcggcctcgg cgcgccgcgc ggcgatgtcc ggcaccacgg cccgggcccg ctccagcagg
      121 tccccgatcg ccggcgtgcg ctgcagaatg gccgtgtcgt ccaacccccg cctcatgccg
      181 cggcccgccc caggcgttcg gccagcagcc gctccagttc gcggcggtaa accttgttgc
      241 tgtgcatcat cggcacctct tcccgggcga tctcaacata ggcgcggggc accttgtacc
      301 ccgagagcct gcgccgcatc tcctgctcga tcacggcgag atcgagcgtc atgccctcgc
      361 gcgcaaccag cgccgcgacc accagttgcc cacgctccgc atcgggcaag ccgaccacat
      421 aggccgaatg aaccccgtcc atccgctgca actcgtcctc gacctcggcg ggcgaaacat
      481 tggccccggc ggtcttgatc atatccccgc cgcgcccgac gaagtggaac cgggcgccgt
      541 cccgcgtgca caggtcgccg gtgtggaaga acccatccgg agtgaaatag gcggcccgct
      601 cgatcttgtg cagggccggc gtcaccggat agccgcgcac ctggatttcc ccggtttctc
      661 catcgaccac ctcgcggccg tcttcgtcca ccacgcgaat gtcatagccg ggcgcccagt
      721 ggtccagcgg cacggccagc ggccagcctt cggcacgcac ctcgtcgccc caggaatagg
      781 cggccagggt ctcgctcatg cccaggcccc aatagggcgg gctcttgagc atctcggcga
      841 tgtcctcggg cgtcagcatc gatcccaggg cgtggcggct gttgatcttg gtcgcctcgg
      901 tacagagccc ggtgaccccg cgcacccagg tcatcatcag gcccatcatc aacccgccca
      961 cccagaacat cggcatgaca agtgggaatt cgggctgtgc gctcatcgac ggaaccatgc
     1021 gcccggacag ccactgggcg cggaacatta ttggtccatg caggtgccgt acgcccttgg
     1081 gcgcggccat cgagcccgag gtatagatct cgatcgcctg atcggtggga tgaacttcgc
     1141 gctcgatctc ggcaaggagt gccgcgtcca cttccgcggc ccgcgcgcgc aggtcggaca
     1201 gggtgccgat ccccggcggc aacccctcac cgctcgagca gatccaccgc aggtacggcg
     1261 ccgctggcag gcgcaggtca cccgcgccag gtgcggccag ttccggcagc gcctcggcca
     1321 gccgctcggc catatcgtgc cccagaaccc ggcgttggac gatcagcccg cccacgtcgg
     1381 actggcgcag cacccgcacc agctcggccg cgcgcagcat ggtgctgatc gggacgggca
     1441 ccgcgccgat gcgtgcgata gcggcatagg tcaccaccca gtccggcccg ttggtcagga
     1501 tgaagccgat ccgcgaaccc ttgcccgccc cgcgggccag cagggcccgg cctagctcgg
     1561 ccgatcgctc gtccagcccg cggaagctta tctcgcgcag ttcaccctcg ccctcggcca
     1621 gccggaaggc gagcttgtcg ccataggtcg cggccgccgc gtggatcaat tgcggaatgg
     1681 tgcgggcaag caccgggtcg gtccaactcc ccggaccgga catcggcggc accgcctcgg
     1741 cccctggctc gacagccttg tccaccacgt cggtcccatg cgtctgactg gacatatcgt
     1801 ctcccgccgg gctcatcgcc ggttgccctc ttaacaccgg gccagccgag cttggcaagc
     1861 cgctcgcgca accggcgcga cgtgcaacgc aaggcatcgc aagtgtgcat ggcatgaccc
     1921 tcccgggcga accggccggg caggcaagat agaggttcca caggccgggc agcccggcca
     1981 attccgggat gcctccggac ccgcgcatcc ccaaccccaa acgaaaaacc cgccgcccgg
     2041 gagggcagcg ggtcttctga tgcgttcggg acgccgctgg cttattcgcc cgcgttgtcc
     2101 tcggcttcat tggcgacaag gtagtccccg gcatcggcat cggtgccgtg ggtctcgccc
     2161 tcgaccgctg ccagcggatc gtcgccgata gcagcttccg ggccctgcgc cagttcggcg
     2221 gcatgctctt ccgcggccgt ttccggcgcg atcaggcttt cctgcagctt gcggtactgc
     2281 gcgcgcagcg cggcatcgcg gctggaggcg gccacgcgca ggcggttcat gcccgcaccg
     2341 gtgcccgccg ggatgagccg gccgacgatc acgttctcct tgaggccgat caggctgtcg
     2401 cgcttgcctt ccacggccgc ctgcgtgagc acgcgggtgg tctcctggaa cgaagccgcc
     2461 gagatgaacg agcgggtctg cagcgaggcc ttggtgatgc cgagcagcac cggcttgccg
     2521 acggcaggcg catggccttc cggcagcttg gcgttgatct cgtccatctc ctcgcggtca
     2581 acctgttcgc cggcgagcag cacggtgtcg ccaccgtcgg tgatctcgac cttctgcagc
     2641 atctggcgaa cgatcacctc gatgtgcttg tcgttgatct tcacgccctg caagcgatag
     2701 acttcctgga tttccgcgac gaggtattcg gccagagcct caaccccgag cacttccagg
     2761 atgtcgtgcg gatcgggcga gcccgagatc aggttgtcgc ccttcttgac ccagtcgccc
     2821 tcctgcacgt cgatgacgcg gctcttgggg atcaggtact cgatcggctc gccctcttcc
     2881 gggatgatcg cgatcttgcg cttggccttg tagtcgcgga cgaattcgat ccggcccgaa
     2941 atcttcgcga tgatcgcgtt gtccttcggg atgcgggctt cgaacagttc ggcgacgcgc
     3001 ggcagaccgc cggtgatgtc gcgggtcttg gcggcttcgc ggctggcgcg ggcaaggatg
     3061 tcacccgcct gcacgtcctg gccatcctcg accgacaggg tcgtgcccgg cgccagcatg
     3121 tagcgcgcgg attcgcccga ggtgtcgtcg tgaagggtca ggcgcggacg caggtcgtcc
     3181 ttcttgg
//
LOCUS       DATNBV010000148         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2953431,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000148 DATNBV010000000
VERSION     DATNBV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2953431"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..618)
                     /locus_tag="VN627_03525"
     CDS             complement(169..618)
                     /locus_tag="VN627_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HXD02356.1"
                     /translation="MPDSPQLYATVRDDFFSARSGPLAEIRLAGTICRACGEPSLGVV
                     PACQACQSTDLAPATLSRDGTLYSYTVVRNRPPGDFKGKVDPFVPYPVGLVELPEGIR
                     VLSVLDCPVDAPRVGMAMTLDVHELYREEDGTPVIAFRFRPSAGENR"
     gene            737..1939
                     /locus_tag="VN627_03530"
     CDS             737..1939
                     /locus_tag="VN627_03530"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXD02357.1"
                     /translation="MREAVIVSTARTAICKAFRGGFNDTDAPVMAGRVIDAAVERAGI
                     DPARIDDLEFGCAVQSGNQGTNIARAGVFASSLPLDIAGAAVDRKCASGLTALAGIAR
                     GIIAGDLDVGVAGGVESVTLTLANPTKLDRVQTAAVKARVPHAYMQMIETAEVVAERY
                     GVSREAQDAFAAESHARAAAAAAAGRFADEIVPLATRRNVHAKDGSIERSEDVVVDAD
                     EGIRAGTTAESLAKLAPVWRDGEIIQQGTSVTAGNASQLSDGASAQVLVERSIAEREG
                     LPILGVFRGFQAAGCLPEEMGIGPVYAIPKLLKRAGLSIEDIGLWEINEAFASQALYC
                     RDRLGINPERLNVNGGGIALGHPFGMTGSRLTGHVLIEARRRGVRYAVVSMYVAGGMG
                     AAALFEVA"
     gene            complement(1960..2064)
                     /locus_tag="VN627_03535"
     CDS             complement(1960..2064)
                     /locus_tag="VN627_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD02358.1"
                     /translation="MERYVRDAMVLPIFGGSTAIQRNNIASLMRLPKG"
BASE COUNT          358 a          649 c          758 g          313 t
ORIGIN      
        1 tcgccggcag atacatccgc gcgcaatagg ccagctggat ctcgcgccag gccatcccgg
       61 catcggtcag cgccatggtc gccgcctcga gccccatttc ggtataggcc ttgtcgccga
      121 agcgaccgaa cgggtggatg ccgaccccgg caatgtagac gcggcgcatc agcggttctc
      181 ccctgcggaa gggcggaagc ggaaggcgat gacgggggtg ccatcctcct cgcggtagag
      241 ttcatggacg tcgagggtca tcgccatgcc gacacggggg gcatcgaccg ggcaatcgag
      301 cacggagagc acgcgaatgc cttcgggcag ttcgaccagg ccgaccggat aggggacaaa
      361 gggatcgacc ttgcccttga agtcgcccgg cggacggttg cgcaccacgg tgtagctgta
      421 gagcgtgccg tcgcgcgaca gggtggccgg cgcgagatcg gtggactggc aggcctggca
      481 ggcaggaacc acgcccagcg agggttcgcc gcaggcgcgg cagatggtcc ccgcaaggcg
      541 gatttccgcg agaggaccgc tgcgcgcgga gaagaaatca tcgcgaaccg tggcgtacag
      601 ctgagggcta tccggcattg cgcgccgctc cgattatgtt acctaacaag cgttagcctg
      661 tgttgggtgg tgcccggaat gtcaaccgga accttggccc aagccgcaca acgaccagag
      721 aggatcgaag gatatcatgc gggaagctgt tatcgtgtcg accgcccgga cggcgatctg
      781 caaggcgttt cggggtggat tcaacgatac cgacgcgccg gtcatggccg ggcgggtgat
      841 cgatgcggcg gtggagcgcg ccgggatcga cccggcgcgg atcgacgatc tcgaattcgg
      901 ctgcgcggtg caatcgggca accaggggac caatatcgcc cgggcgggcg tgttcgcctc
      961 cagcttgccg ctcgacattg ccggcgcggc ggtcgaccgc aagtgcgcct cggggctgac
     1021 ggcgctggcc ggcattgccc ggggcatcat tgccggcgat ctcgacgtgg gcgttgccgg
     1081 aggggtcgaa tcggtgaccc tgacgctggc caatccgacc aagctcgacc gggtgcagac
     1141 ggcggcggtg aaggcgcggg ttccccatgc ctacatgcag atgatcgaga ccgccgaagt
     1201 ggtggcggag cgctatggtg tcagccgaga ggcccaggat gccttcgccg ccgagagcca
     1261 cgcccgcgcg gcggcggcgg ctgcggccgg ccgctttgcc gacgagatcg tgccgcttgc
     1321 cacccggcgc aacgtccatg ccaaggacgg ctcgatcgag cggagcgagg acgtggtggt
     1381 cgacgccgac gagggcatcc gagccggcac gacggcggaa agcctggcca agctcgcccc
     1441 ggtgtggcgc gatggcgaaa tcatccagca gggaaccagc gtgacggcag gcaatgccag
     1501 ccagctctcc gatggcgctt cggcgcaggt gctcgtcgag cgcagcatcg ccgaacgcga
     1561 ggggctgccg atcctgggcg tgttccgcgg gttccaagcg gcgggatgcc ttcccgagga
     1621 gatgggcatc ggcccggtct acgcgatccc caagctgctc aagcgcgccg gcctgtccat
     1681 cgaggacatc ggcctgtggg agatcaacga ggccttcgcc agccaggcgc tctattgccg
     1741 cgaccggctc gggatcaatc cggagcgcct gaacgtgaac ggtggcggga tcgcgctggg
     1801 ccatcccttc gggatgaccg gcagccgctt gaccgggcat gtgctgatcg aggcaaggcg
     1861 ccgcggcgtg cgctatgccg tcgtttcgat gtacgtggcc ggcgggatgg gcgcggccgc
     1921 gctgttcgaa gtggcctgag gtgaggaggg gccggcccct cagcccttgg gcaaacgcat
     1981 caggctggcg atattgttgc gctggatcgc ggtggagccg ccaaagatcg gcagcaccat
     2041 ggcatcgcgc acataacgct ccatgtcgaa ctcatccg
//
LOCUS       DATNBV010000149         3405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3917906,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000149 DATNBV010000000
VERSION     DATNBV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3917906"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VN627_03540"
     CDS             <1..467
                     /locus_tag="VN627_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXD02359.1"
                     /translation="LQSVVVIGATNRPTLVDPALLRPGRFDELVYVGTPDEAGREHIL
                     KIHTARMPLASDVDLKAVARETERFTGADLEDVVRRAGLVAIRRMGRDVGAVSAADFA
                     EALTDSRATVTAEIEEEYGRMKGELKRRAAEVAPIGFLAPGMVQPTREKKHP"
     gene            complement(599..1642)
                     /locus_tag="VN627_03545"
     CDS             complement(599..1642)
                     /locus_tag="VN627_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HXD02360.1"
                     /translation="MKLPQVPDFRPILVQVPEAVIAGGVAVIVALALHFVLFRVLLRL
                     SRLSHLESDEIVVERLRGPFRWALLAIAIEIAGESSALIARVWGAVDRFVIPALLGWI
                     ALRLVRAYALAVDSRARLEADTLAARSRRTRIAILSRSARFIIVFVTVALMLLSIPGV
                     RNVGVTLITSAGLAALAVGAAAQPALKSLIAGIQMALTEPIRIDDHVVVEGESGRVED
                     IRMTYVVIRTGDQRRLIVPTARFLETTFQNWTRVGGGITGSVLLPVNPGTAIQPIRTA
                     YEEILSHRKEWDKRSGALQVSGSWIDRIELKLVMSAADPAALATLQLTVREEMLEWLR
                     VNMPEAVAVPTAP"
     gene            complement(1689..3011)
                     /locus_tag="VN627_03550"
     CDS             complement(1689..3011)
                     /locus_tag="VN627_03550"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HXD02361.1"
                     /translation="MASPAPPSGPAPGEPRPLHRGPGLPTLLLLAFVAAALIGSVALT
                     WQTVAAERSQRAQAERTNAVLVAVRDILSTATNAETGQRGYFITLDRRYLGPYLVARE
                     QYRPNFARLRALIGDQPSPRQRDLLDEIQTLDEAKFAEMAETVDLIARGNLLEARARV
                     LTDEGQDVMERLRRAIGELESLEFSALNRSSAEAQSAEQRIVPLLSGLLVTILLALAL
                     GLMQVIRAADAEGRAEGAAELALARDRADLLAHELNHRVKNLFAVVLAIVKMSARGAP
                     EAKPVVDKIAERIHALLTAHEVTQGAPMRRSANLFDLIETALRPYQSAENRAVLDGPA
                     IDLPESAVVPLGLVLHELTTNAVKYGAWSRPGGELAVRWTRSDHHIRLDWQERAAEPC
                     PPPPGGKPAHRGFGSTLIDGSARQLGGTIERSFTPRGVEVRIDLPLGE"
     gene            complement(3155..>3405)
                     /locus_tag="VN627_03555"
     CDS             complement(3155..>3405)
                     /locus_tag="VN627_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HXD02362.1"
                     /translation="SADIIKRAMVRMMPALAAAGLGHVRMLLQVHDELVFELPQADAA
                     AAAPVIERVMAEAALPSVKLDVPLGIEIGTGPNWGAAH"
BASE COUNT          515 a         1071 c         1328 g          491 t
ORIGIN      
        1 aactccagag cgtagtggtg atcggcgcga ccaaccggcc gacgctggtc gatccggccc
       61 tgctgcggcc cgggcggttt gacgagctgg tctatgtcgg cacgccggac gaggccgggc
      121 gcgagcatat cctgaagatc cacacggccc gcatgccgct ggccagcgac gtggacctga
      181 aggcagtggc gcgcgagacc gagcgcttca ccggcgcgga cctggaggac gtggtccgcc
      241 gggcgggcct ggtcgccatc cgccggatgg ggcgcgacgt gggcgcggtc agtgccgccg
      301 acttcgccga ggccctgacc gacagccgcg ccaccgttac ggccgagatc gaggaggaat
      361 atggccggat gaagggcgag ctgaagcggc gggcggcgga agttgccccg atcggcttcc
      421 tcgcgccggg catggtgcaa cccacgcggg agaagaagca cccctgatga tccgccccgc
      481 tcgtggggag gggatcgccg gaacggcggt ggagggggtt tgccagcgca ggccgatctt
      541 gcctgccagc cccctccgtc agccctgcgg gctgccacct ccccgctagt ggggaggatc
      601 agggggcggt cggcactgcc acggcttcgg gcatgttgac ccgcagccat tcaagcattt
      661 cctcgcggac cgtcagctgc agggtggcga gggccgcggg gtcggcggcg ctcatcacga
      721 gcttgagttc gatccggtcg atccacgagc cggagacctg gagggcgccg gagcgtttgt
      781 cccattcctt gcggtggctg aggatttcct cgtaggcggt gcggatcggc tggatcgcgg
      841 tgccggggtt gaccgggaga aggaccgagc cggtgatccc gccgccgacg cgggtccagt
      901 tctggaaggt ggtttcgaga aaccgggccg tggggacgat caggcggcgc tggtcgccgg
      961 tgcggatgac gacgtaggtc atccggatat cctcgacccg cccgctttcg ccttcgacga
     1021 cgacgtgatc gtcgatccgg atcggctcgg tcagcgccat ctggatcccg gcgatcagtg
     1081 acttgagcgc gggctgggcg gcggcgccga cggcgagcgc ggcgaggccc gccgaggtga
     1141 tcagggtgac gccgacattg cgcacgcccg ggatcgacag cagcatcagc gcgacggtga
     1201 cgaagacgat gatgaaccgt gccgagcgcg agaggatcgc gatgcgggtg cggcggctgc
     1261 gggcggccag ggtatcggcc tcgagccggg cgcggctgtc aaccgccagg gcataggccc
     1321 ggaccagccg cagcgcgatc catccgagca gggcaggtat gacgaagcgg tcaaccgcgc
     1381 cccagacgcg ggcgatcagc gccgagcttt cgcccgcgat ctcgatggcg atggcgagca
     1441 gcgcccagcg gaacggcccg cgcagccgtt cgaccacgat ctcgtcgctt tcgaggtgcg
     1501 agaggcggga gaggcgcagg agcacgcgga acaggacgaa gtgcagcgcg agggcgacga
     1561 tcaccgccac cccgccggcg atcacggcct cgggcacctg gacgaggatc gggcggaaat
     1621 cgggaacctg ggggagcttc atgggcctct ggttaggacg gggcagccat cagggcaatt
     1681 gcggcgactt actcgccgag cggcagatcg atcctgacct cgaccccgcg gggcgtgaag
     1741 ctgcgttcga tcgtgccgcc gagctgacgc gcggagccgt cgatcagggt ggagccgaaa
     1801 ccgcggtggg caggtttacc gccggggggc ggcgggcagg gctcggcggc gcgctcctgc
     1861 cagtcaagcc ggatgtgatg atcggaccgt gtccagcgga cggcgagctc cccgcccggg
     1921 cgcgaccagg cgccatattt gaccgcattg gtggtgagtt cgtgaagcac gagcccgagc
     1981 gggaccacgg cgctttccgg caggtcgatc gccggaccat cgagcacggc gcggttttcg
     2041 gccgactggt aggggcgcag ggccgtctcg atcaggtcga acaggttcgc gctgcggcgc
     2101 atgggggcgc cctgggtcac ctcgtgggcg gtgagcaggg cgtggatgcg ctcggcaatc
     2161 ttgtcgacca cgggcttggc ctcgggtgcg ccccgggcgc tcattttcac gatggcgagg
     2221 accaccgcga agaggttctt gacccggtgg ttgagctcgt gggcgaggag atccgcccgg
     2281 tcgcgggcga gggccagctc ggcggcgcct tcggcacggc cctcggcatc ggcggcccgg
     2341 atcacctgca tcaggcccaa ggcgagggcg agcaggatcg tcaccagcag gcccgagagc
     2401 agggggacga tccgctgctc ggccgactgt gcctcggcgc tcgaccggtt gagggcggag
     2461 aattccaggc tttccagttc cccgatcgcg cggcgcaggc gctccatcac gtcctgtccc
     2521 tcgtcggtga ggacgcgggc gcgcgcttcg agcaggttgc ccctggcgat gaggtccact
     2581 gtttcggcca tctcggcgaa cttcgcttcg tcgagcgtct ggatctcgtc aagcaggtcg
     2641 cgctggcgcg ggctgggctg gtcgccgatc agggcgcgca agcgggcgaa gttggggcgg
     2701 tactgctcac gcgcaacgag gtaggggcca agatagcgcc ggtcgagcgt gatgaaatag
     2761 ccgcgctggc cggtctcggc attggtcgcg gtgctcagga tatcgcgcac cgccacgagc
     2821 acggcattgg tgcgttcggc ctgggcgcgc tgggagcgct cggcggcgac ggtctgccag
     2881 gtgagcgcga ccgaaccgat cagcgccgcg gcaacgaaag cgagcagcag gagcgtgggc
     2941 aggcccgggc cgcgatggag cgggcggggc tcgcccgggg ctgggccaga ggggggagcg
     3001 gggctggcca tgaggcgatt ctaggggaaa gtcacggggt gtctactggg ggtgaggttg
     3061 ctggcgggcg ccgcgctgtc accctgccca accttcgcca ggccgcgctg cggccaagct
     3121 gcggtatccc tcccccatcg cgggggaggg ctggtcagtg cgccgcgccc cagttggggc
     3181 cggtgccgat ctcgatgccg agcggcacgt cgagctttac cgagggcagg gcggcttcgg
     3241 ccatgacccg ctcgatcacc ggggcggcgg cggcggcatc ggcctggggc agttcgaaga
     3301 cgagttcgtc gtggacctgc agcagcatgc ggacgtggcc gaggccggca gcggcgaggg
     3361 caggcatcat ccggaccatg gcgcgcttga tgatatcggc gctgg
//
LOCUS       DATNBV010000150         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4571219,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000150 DATNBV010000000
VERSION     DATNBV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4571219"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VN627_03560"
     CDS             complement(<1..376)
                     /locus_tag="VN627_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXD02363.1"
                     /translation="MTARHILIPLHDFSAGGTEIIAFRLVEAWLKAGRRITIVAGAAD
                     GPMLARVPAGAAVEVLSPAIPRSPVSRLRLGVPLAALARRLKPDAVMIPGNFHFILAR
                     SLRAAMPGVPIVAKISNPLIPAN"
     gene            495..1664
                     /locus_tag="VN627_03565"
     CDS             495..1664
                     /locus_tag="VN627_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HXD02364.1"
                     /translation="MSETIRPFTYVAEAGELERLKRRIALTRWPERETVADRSQGEPL
                     AELRDLVDYWQSGYDWRPCEARLNALPQYLTEIDGLDIHFIHVRSSHEQARPLILTHG
                     WPGSVLEFLKTIPALTQPEAHGGQAEDAFHVVIPSLPGFGFSGKPASTGWNLERIGRA
                     WSTLMARLGYDRWFAQGGDWGGLMTGRIASLPVPGCAGVHLNLIPGFPLPEDLENPSP
                     AAQRALKRGQEHQQWGMGYSTLQQTRPQTIGYSLVDSPVGLAGWIMEKIAEWTDNQGS
                     PYDALSRDEILDNITLYWLTATGASSARIYWEVNQSGYYQNAMFAPTLLPTAYTLFPR
                     EMAPTPPEWLRHHTNLVYANEVDRGGHFAAWEQPELFVEEVRAAFGRMSLTGDAK"
     gene            1661..>2549
                     /locus_tag="VN627_03570"
     CDS             1661..>2549
                     /locus_tag="VN627_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HXD02365.1"
                     /translation="MSEAIIPFTVAIPQAALDDLAERLDLARWPEAETVDDWSQGVPL
                     AVLQPLVEYWRNGYDWRRCEARLNSLPQFMTEIDGLDIHFIHVRSVHANATPLLLTHG
                     WPGSILEFVEAIPRLVDPEAHGGTAADAFHVVIPSLPGYAFSAKPEQTGWGLERTSRA
                     WGTLMARLGYDRWFAQGGDWGSAVTTTIGQLGVPGCAGVHVNLMYAQPPERALTDPTP
                     EEAHALERYRFYNEDDHGYALEQRTRPQTVGYSLVDSPVGLAAWILEKVYAWTDNAGS
                     PFDALSRDQVLDEITLYWLT"
BASE COUNT          432 a          851 c          843 g          423 t
ORIGIN      
        1 tgttggccgg gatcagcggg ttgctgatct tggccacgat cggcacgccc ggcatggcgg
       61 cgcgcagcga tcgcgcaagg atgaagtgga agttgccggg aatcatcacg gcatcgggtt
      121 tcagccgccg ggccagggcg gccagcggca cgccgaggcg caggcgcgag accgggctgc
      181 gcggtatcgc cggagagagg acttccaccg ctgcgccggc cgggacacgc gccagcatcg
      241 ggccatcggc cgcgccggcg acgatcgtga tccgccgtcc ggccttgagc caggcctcga
      301 ccaggcggaa ggcgatgatc tccgtgcccc cggcggagaa gtcgtggagc gggatgagga
      361 tatggcgcgc agtcatggag gcactttctt gccgttccgc ctttccgcgc ccgcgagaaa
      421 atggcaagcc cgcagcatag ggccagccgg gacagcggtt tgcggcgggg ccaaggcgga
      481 ggaaaaacag gtccatgtca gagacaatca ggccgttcac ttacgttgcc gaagccggcg
      541 aactggagcg gctgaaacgg cgaatcgcac tgacgcgctg gcccgagcgc gagacagtgg
      601 ccgaccgcag ccagggcgag ccgctggcag agctgcggga cctggtcgac tattggcaga
      661 gcggctacga ttggcgcccc tgcgaggccc ggctcaatgc cctgccgcag tacctcaccg
      721 agatcgacgg gctggatatc catttcatcc atgtccgctc gagccacgaa caggcgcgtc
      781 cgctgatcct cactcacggc tggcccggtt cggtgctgga gttcctcaag accatccccg
      841 cgctgacgca gcccgaggct catggcggcc aggcggagga tgccttccac gtggtcatcc
      901 catcgcttcc cgggttcggc ttttcaggca agccggcgag cactggctgg aatctcgaac
      961 gtatcggccg ggcctggagt acgctgatgg cgcggctcgg ctatgatcgg tggttcgcgc
     1021 aagggggtga ctggggcggg ctgatgaccg ggcgcatcgc ctcgctgccg gttcctggct
     1081 gtgccggtgt ccacctcaat ctcattcccg gctttcccct gccggaggat ctcgagaacc
     1141 cgtcgcctgc cgcgcagcgg gcgctgaagc gggggcagga acatcagcag tggggtatgg
     1201 gctactctac cctgcagcag acccggccac agacaatcgg ctattccctc gtcgattctc
     1261 ccgttggcct cgccggctgg atcatggaaa aaatcgcgga gtggaccgat aaccagggta
     1321 gcccctacga tgccctgagc cgcgacgaga tcctcgacaa catcacgctc tactggctca
     1381 ccgccaccgg ggcctcgtcg gcccggatct actgggaggt caaccagtcc ggctactacc
     1441 agaacgccat gttcgcgccg accctgcttc ccacggccta taccctgttc ccgcgcgaaa
     1501 tggcgccaac gccgcccgaa tggctgcgcc atcacaccaa ccttgtctac gcgaacgagg
     1561 tggaccgggg tggccatttc gccgcctggg agcagccgga gctgttcgtg gaagaggtgc
     1621 gggccgcctt cggccgcatg tcgctgaccg gagacgcgaa atgagcgagg ccatcatccc
     1681 tttcaccgtc gccatccctc aggccgcgct cgatgatctg gccgaacggc tcgatcttgc
     1741 ccggtggccc gaggctgaga ccgtggacga ctggagccag ggcgtaccgc tggccgtcct
     1801 gcaaccgctc gtcgagtact ggcgcaatgg ctatgactgg cgccgctgcg aggccaggct
     1861 caacagcctg ccgcagttca tgaccgagat cgacggcctg gacattcact tcatccacgt
     1921 gcgctcggtc catgccaatg ccactccgct gctgctgacc cacggctggc cgggctcgat
     1981 cctggaattc gtcgaagcca ttccgcgcct ggttgatccc gaagcccatg gcggcacggc
     2041 ggccgatgcc ttccacgtcg tgatcccgtc actccccggc tatgcctttt ccgccaagcc
     2101 ggagcagacc gggtgggggc tggaacgtac gtcgcgggcc tggggcacac tgatggcacg
     2161 gctcggctat gatcgctggt ttgcgcaagg tggcgattgg ggctcggcgg tgacgaccac
     2221 catcgggcag ctgggcgttc ccggctgtgc gggcgttcac gtcaacctga tgtatgcgca
     2281 gccgcctgag cgcgcgctga ccgacccgac gccggaagaa gctcatgccc tggaacgcta
     2341 ccggttctac aacgaggacg accatggcta cgccctcgag cagcggaccc ggccgcagac
     2401 cgtcggttac agtctggtcg attctcctgt cgggctggca gcgtggatcc tcgaaaaggt
     2461 ctacgcctgg accgacaacg ccggcagccc gttcgacgcg ctgagccgcg accaggttct
     2521 tgacgagatc accctgtact ggctcaccg
//
LOCUS       DATNBV010000151         6466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_736043,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000151 DATNBV010000000
VERSION     DATNBV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736043"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VN627_03575"
     CDS             complement(<1..858)
                     /locus_tag="VN627_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HXD02366.1"
                     /translation="MAKPKRRYVCSACGSVATRWQGQCADCGEWNSLTEDAPQTVFSA
                     KHDLSSGGRPIAFEPLDGRGEVLARRSTGLAEFDRALGGGLVPGSAILMGGDPGIGKS
                     TLLLQASARVASAGGAVVYISGEEAAGQVRLRAERLGLGKAPVSLASATSVRDILTTL
                     GEMEPPALLVIDSIQTMHSDQIEGAPGTVSQVRGCAFELIRYAKESGCALVLVGHVTK
                     DGNIAGPRVLEHMVDVVMAFEGERSHQYRILRSLKNRFGPVDEIGVFAMAGAGLEEVS
                     NPSSLFLSGR"
     gene            929..3256
                     /locus_tag="VN627_03580"
     CDS             929..3256
                     /locus_tag="VN627_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like protein"
                     /protein_id="HXD02367.1"
                     /translation="MRQKELRIGLVCYGGISLAVYMHGVTKELWKLVRASRAFHSGEA
                     CPDDGSPHDTEHVYHRLLQQAAERHGMGLRVLADIVAGASAGGINGVFLAQAIHSGQS
                     LEPLTELWLERADVDVLIDPSAKPWWRFAKFWAAPLIWFVLSRPGNVVAQSVARETRK
                     EVRAKLSRLIRSRWFEPPFGGLGFSRLIADALEAMAKSVSCPPLLPAGYPLDLFVTAT
                     DFKGHKETIRLHSPRLVEESEHRLSIGFRALVPTEPGRDLADPLELTFAARATASFPG
                     AFPALRLAEIDALAAERGLTWAGRTDFARRTMPEHAVSGDVEQVSLIDGSVLVNAPFA
                     EAMAVLRDRPAQREVDRRFVYIDPRPDRVGGLQPDDPRPPGWFSVIFGSLSSIPREQP
                     IRDNLEVLAAQSREMARLQSIVGALRPEVEESVEKALGHTLFLDRPTPKRLKAWRDKS
                     HEAARRKAGFAWHAYLEVKVAGIVETIATLVCQAVPELPHEAVLTALEGHLQRQGLGR
                     LASPGGLISPDAVTFLRSHDLAFRIRRLRLLARRLSQEWDGDPEVSDEARHEARDAVY
                     RALSLYFDREPLTLLGADFAEAAAKVVTNPAAVFDILEKRRQLPATDLVVDALLAEAL
                     GRMVPALRRKVLLSYLGFPWFDAVTLPLLRGEGLTEFDPVKVDRISPDDCSSIRPGGT
                     NATLRGVEFYNFGAFFSRAYRENDYLWGRLHGAERMIDLVSSALPGAEPMTAQELKAF
                     KREAFLAILTEEEKRLKADPGLVTGIRAEVEKAMA"
     gene            3470..3757
                     /locus_tag="VN627_03585"
     CDS             3470..3757
                     /locus_tag="VN627_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXD02368.1"
                     /translation="MGGWVYIMSNAPHGTLYIGVTANLPARIWQHREGTGSEFCQKHG
                     LTRLVYVETYDRIEDAIAREKAMKRWKRAWKLRLICRDNPNWNDLFDQINA"
     gene            complement(3972..5090)
                     /gene="dnaJ"
                     /locus_tag="VN627_03590"
     CDS             complement(3972..5090)
                     /gene="dnaJ"
                     /locus_tag="VN627_03590"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXD02369.1"
                     /translation="MSAEIDYYELLEVERGADDATLKSAYRKLAMRFHPDKNPGCEHS
                     ENRFKQINEAYECLKDPQKRAAYDRYGHAAFQQGGGFQGGGEGFSDIGDIFETIFGNA
                     FGGGQRQARRGADLRYDMEISLDEAFHGKNATIDIEVAATCDPCGGSGSTTGTGTRRC
                     TMCGGHGKVRAQQGFFMVERTCPTCQGRGEVIENPCRACGGEGRVDKPQTLDVQIPAG
                     VDTGTRIRLGGKGEAGPFGAPAGDLYIFIHVKRHKVFERDGTTLLTRVPISFTTAALG
                     GEIEIPGLDGTRHEISIPAGIQTGKQLRQRGAGMPVLQGRGRGDMVIEIQVETPTKLT
                     ARQKELLRELQATETGEECPQSRGFFDRLKDAWSDLTE"
     gene            complement(5157..>6466)
                     /gene="dnaK"
                     /locus_tag="VN627_03595"
     CDS             complement(5157..>6466)
                     /gene="dnaK"
                     /locus_tag="VN627_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXD02370.1"
                     /translation="SILEIGDGVFEVKSTNGDTFLGGEDFDTQVVEYLAEQFKKKEGM
                     DLRTDKLALQRLKEAAEKAKIELSSAQTTEINLPFITARMEGGSTTPLHLVETITRSD
                     LERMVADLIKRTIAPCQKALADAGLKASDIDEVVLVGGMTRMPKVREAVKDFFGKEPH
                     TGVNPDEVVAMGAAIQAGVLQGDVKDVLLLDVTPLSLGIETLGGIMTKMIDRNTTIPT
                     KKSQIYSTAEDNQQAVTIRVFQGEREMAQDNKLLGQFDLVGIPPARRGVPQIEVTFDI
                     DANGIVNVSAKDKGTGKEQQIRIQASGGLSDSDIDQMVRDAEKFAEEDKKRREAAEAR
                     NNADSLVHATERQLEENGDKIDAGLKGEIETAIAEAKAAIESGDAEAMTAKTQALTEK
                     AMKMGQAIYEKEQATQASPGAEAPAGDDDVVDAEFSEVDDHKA"
BASE COUNT          999 a         2390 c         1953 g         1124 t
ORIGIN      
        1 acgcccggaa aggaacaggc tcgagggatt gctgacctcc tccagcccag cgcccgccat
       61 ggcaaacacg ccgatctcgt ccaccggccc gaaccggttc ttgaggctgc gcaggatgcg
      121 gtactggtgg ctgcgctcac cctcgaaggc catgaccaca tcgaccatgt gctccagcac
      181 gcgcggcccg gcgatgttgc cgtccttggt cacatggccc accagcacca gtgcgcagcc
      241 gctctccttg gcatagcgga tcagctcgaa agcgcagccg cgcacctggc tgaccgtccc
      301 cggcgcgccc tcgatctggt cggaatgcat cgtctggatc gaatcgatga ccagcaacgc
      361 gggcggctcc atctcgccca gcgtggtcag gatatcccgc acgctggtcg ccgaagccag
      421 gctcaccgga gccttgccca accccagccg ctcggcccgg agccgcacct gcccggccgc
      481 ttcctcgccg ctgatataga ccaccgcccc gcccgccgag gcgacccgcg ccgaagcctg
      541 gagcaacagg gtcgatttcc cgatccccgg atcgccgccc atcaggattg ccgaacccgg
      601 caccagcccg ccgcccagcg cccggtcgaa ctcggccagc ccggtggacc gccgcgccag
      661 cacctcgccc ctgccgtcca gcggctcgaa cgcaatcggc cgccccccgc tggaaagatc
      721 gtgcttggcc gaaaacaccg tctgcggcgc atcttccgtg agcgagttcc attccccgca
      781 atcggcgcac tgaccctgcc agcgcgttgc gacagacccg caggcggagc acacataacg
      841 gcgtttcggc ttggccatgg cgctcgccta acaagaacgg aacaagaacg caattgccac
      901 gcgcggaatt tctgtccaca cccccgccat gcggcagaaa gaactgcgga tcggcctcgt
      961 ctgctacggc ggcatcagcc tcgccgtcta catgcacggg gtaacgaagg aactgtggaa
     1021 actcgtccgc gccagccggg ccttccattc gggcgaagcc tgccccgatg acggctcccc
     1081 ccacgatacc gaacacgtct accaccgcct gcttcagcag gccgccgagc gccacggcat
     1141 gggcctgcgc gtcctggccg atatcgtcgc cggggccagc gcaggcggga tcaacggggt
     1201 gttcctcgcc caggcgatcc attccgggca aagcctcgaa ccgctcaccg aactgtggct
     1261 cgaacgggcc gacgtcgatg tcctgatcga tccttccgcc aagccttggt ggcgcttcgc
     1321 caagttctgg gccgcgccgc tcatctggtt cgtgctcagc cgtccgggca atgtcgttgc
     1381 ccaaagcgtc gcgcgcgaaa cccgcaagga agtgcgcgcc aagctctcgc gcctgatccg
     1441 ctcgcgctgg ttcgaaccac ccttcggcgg cctcggcttc tcccgcctca ttgccgacgc
     1501 gctggaggcc atggcgaaga gcgtgtcctg cccacccctg ctccccgccg gctacccgct
     1561 cgacctgttc gtcacggcaa cagacttcaa gggccacaag gaaacgatcc gcctccattc
     1621 gcccaggctg gtggaggaaa gcgagcaccg cctttcgatc ggctttcgcg ccctcgtccc
     1681 caccgaaccg gggcgcgatc tcgccgaccc gctcgagctc accttcgccg cccgcgcaac
     1741 ggccagcttc cccggcgcct tccccgccct gcgcctcgcc gagatcgacg cgctcgccgc
     1801 cgaacgcggc ctcacctggg ccggccgcac cgatttcgcc cgccgcacca tgcccgaaca
     1861 cgctgtttcg ggcgatgtcg agcaggtctc gctgatcgac ggctcggtcc tggtcaacgc
     1921 cccctttgcc gaagccatgg ccgtcctgcg cgatcgcccg gcccagcgcg aggtcgatcg
     1981 ccgcttcgtc tatatcgatc cccggccgga ccgggtcggc ggcctccagc cggatgatcc
     2041 gcgcccgccg ggctggttct cagtgatctt cggctcgctc tcctcgatcc cccgcgaaca
     2101 gccgatccgt gacaacctgg aggtcctcgc cgcccagtcg cgcgagatgg cccggctgca
     2161 atccatcgtc ggcgccctgc gcccggaagt ggaggaatcg gtcgaaaagg cgctcggcca
     2221 cacgctcttc ctcgatcggc cgacccccaa gcgcctcaag gcctggcgcg acaagtcgca
     2281 cgaggctgcc cggcgcaagg ccggcttcgc ctggcacgcc tatctcgagg tcaaggtcgc
     2341 cggcatcgtc gagaccatcg ccacgctcgt ctgccaggcc gtgcccgagc tgccgcacga
     2401 agccgtgctc accgcgctcg aaggccacct ccagcgccag ggcctcggcc gcctcgcctc
     2461 gcccggcggc ctgatcagtc ccgatgccgt caccttcctc aggtcccacg acctcgcctt
     2521 ccgcatccgc cgcctgcgcc tccttgcccg gcgcctcagc caggagtggg acggcgatcc
     2581 cgaagtgtcc gacgaagccc gccacgaagc gcgcgacgcg gtctaccgcg ccctgtcact
     2641 ctatttcgac cgtgagccgc tgaccctgct cggcgccgat ttcgccgagg ccgcggccaa
     2701 ggtggtcacc aaccccgccg ccgtgttcga cattctcgaa aagcgccgcc aactcccggc
     2761 gaccgatctc gtcgtcgatg ccctgctggc cgaggcgctc ggcaggatgg tccccgccct
     2821 gcgccgcaag gtcctgctgt cctacctcgg tttcccgtgg ttcgatgccg tcaccctccc
     2881 cctcctgcgc ggcgaggggc tgaccgaatt cgatccggtc aaggtcgatc gcatctcgcc
     2941 cgacgattgc tcatcgatcc gccccggcgg caccaacgcc accctgcgcg gagtggagtt
     3001 ctacaatttc ggcgcgttct tctcccgcgc ctaccgcgag aacgactacc tctggggccg
     3061 cctccacggc gccgaacgca tgatcgacct cgtctcctcg gccctgcccg gcgcagaacc
     3121 gatgacggca caggagttga aggcattcaa gcgcgaagcc ttcctcgcaa tcctcaccga
     3181 agaggaaaag cgcctgaagg ccgatccggg gctggtgacg ggaatcaggg ccgaggtgga
     3241 gaaagcgatg gcttaacccc cctcgcctcc acaaaaagcc gtcgcccctg cccttcgggc
     3301 gggccggccg gcccctcagg ataaactgcc cttcgggcat gcaggggggc cagcccagcc
     3361 tcgcgtctaa ccgctaggag tgcggttgtg ggcgagcttg ggataggccc ctgcctccgc
     3421 aggggcgacg gcaattgatc gcaaagccgg ttctgttatc atttcagcga tgggcgggtg
     3481 ggtctacatc atgagcaatg ccccccacgg cacgctctac atcggagtaa ccgccaatct
     3541 ccccgcccgc atctggcaac accgcgaggg caccggctcc gaattctgcc agaagcacgg
     3601 cctcacccgc ctcgtctacg tcgaaaccta cgaccggatc gaggatgcca tcgctcgcga
     3661 aaaagccatg aaacgctgga aacgcgcctg gaaactgcgc ctgatctgcc gcgacaaccc
     3721 gaactggaac gacctgttcg accagatcaa cgcgtaagtc acaacagcgt tcaccaatat
     3781 ccgtcgcccc tgcgcaggca ggggcccagc ccaagcttgc ccacaaccgc agcggcaccg
     3841 ttcttgcgcg aggctggaca ggcccctgcc tgcccgaagg gcagttcatc ctgagcggcg
     3901 ggccggctgg ccagccgaag ggcaggggcg acggtgctaa acgagctcag atcgcgggtg
     3961 acaaagcccc cctactccgt caggtcgctc cacgcgtcct tcagccggtc aaaaaaaccg
     4021 cggctctgcg ggcattcctc cccggtttcc gtggcctgca gctcgcgcag cagttccttc
     4081 tggcgggcgg tcagcttggt tggcgtttcg acctggattt cgatcaccat gtcgccccgg
     4141 ccccggccct gcagcaccgg catgcccgcg ccacgctggc gcagctgctt gcccgtctgg
     4201 atgccggcgg gaatgctgat ctcatgccgc gtgccgtcaa gcccggggat ctcgatctct
     4261 ccgcccagcg ccgccgtggt gaagctgatc ggcacgcggg tgagcagggt ggtcccgtcg
     4321 cgctcgaaca ccttgtgccg cttgacgtgg atgaagatgt agaggtcgcc cgccggcgcg
     4381 ccaaacggcc cggcttcgcc cttgccgcca aggcggatgc gagtgccggt atccacgccc
     4441 gccgggatct gcacgtcgag cgtctgcggc ttgtcgaccc gtccttcgcc tccgcaggcg
     4501 cggcagggat tctcgatcac ctcgccgcgg ccctggcagg tcgggcaagt ccgctcgacc
     4561 atgaagaaac cctgctgcgc gcgcaccttg ccgtggccgc cgcacatggt gcagcgccgc
     4621 gttccggtgc ccgtcgtcga gcccgaaccg ccgcacgggt cgcaggtagc cgccacttcg
     4681 atgtcgatgg tagcgttctt gccgtggaac gcctcgtcga ggctgatctc catgtcatag
     4741 cgcaagtccg ccccgcgacg ggcctgacgc tggcccccgc cgaaggcgtt gccgaagatc
     4801 gtctcgaaga tgtcgccgat gtcggaaaag ccttcgccgc cgccctggaa gccgccgccc
     4861 tgctggaagg cggcatggcc atagcggtca taggcggcgc gcttctgcgg atccttgagg
     4921 cattcgtagg cctcgttgat ctgcttgaag cggttttccg aatgctcgca gcccggattc
     4981 ttgtccgggt gaaacctcat cgccagcttg cgataggcgc tcttgagcgt ggcgtcgtcc
     5041 gccccccgct caacctccag tagttcgtaa tagtcgattt cagctgacat gacccgcccc
     5101 taacgcgttc cgccgcccac gaaaatacgc gagcggcgga agctgtgcat tgccgatcag
     5161 gccttgtggt cgtcgacttc cgagaactcg gcgtcgacca cgtcgtcgtc acccgcggga
     5221 gcctccgcac ccggagatgc ctgggtcgcc tgctccttct cgtagatcgc ctggcccatc
     5281 ttcatggcct tctcggtgag agcctgggtc ttggcggtca tcgcttccgc gtcgccgctc
     5341 tcgatcgcgg ccttggcctc ggcgatggcg gtctcgatct cgcccttgag cccggcgtcg
     5401 atcttgtcgc cgttctcttc cagctggcgc tcggtggcat ggaccaggct gtcggcattg
     5461 ttgcgggcct cggccgcctc acggcgcttc ttgtcctctt cggcaaactt ctcggcatcg
     5521 cgcaccatct ggtcgatgtc gctgtccgaa agaccgccgg aggcctggat gcggatctgc
     5581 tgctccttgc cggtgccctt gtccttggcg ctcacgttca cgatgccgtt ggcgtcgatg
     5641 tcgaaggtca cctcgatctg cgggacgccg cgccgcgccg gcggaatgcc gacgaggtcg
     5701 aactgcccga gcagcttgtt gtcctgcgcc atctcgcgct cgccctggaa cacgcggatc
     5761 gtcaccgcct gctggttgtc ctcggcggtc gagtagatct ggctcttctt ggtcgggatc
     5821 gtggtgttgc ggtcgatcat cttggtcatg atcccgccca gcgtctcgat tcccagcgac
     5881 agcggggtca cgtcgagcag cagcacgtcc ttgacgtcgc cctggagcac gccggcctgg
     5941 attgccgcgc ccatggcgac gacctcgtcc gggttcacgc ccgtgtgggg ctccttgccg
     6001 aagaagtcct taaccgcctc gcgcaccttg ggcatgcggg tcatgccgcc gacgaggacg
     6061 acctcgtcaa tgtccgaagc cttgagcccg gcatcggcca gcgccttctg gcacggggcg
     6121 atggtgcgct tgatcaggtc ggccaccatg cgttccaggt cggagcgggt gatcgtctcc
     6181 accaggtgca gcggagtcgt gctgccgcct tccatgcgcg cggtgatgaa cggcaggttg
     6241 atctcggtgg tctgcgcgct cgacagctcg atcttggcct tctcggcggc ttccttcagc
     6301 cgttgcaggg caagcttgtc ggtccggagg tccatgccct ccttcttctt gaactgctcg
     6361 gccaggtatt cgaccacctg ggtgtcgaag tcctcgccgc ccaggaaggt gtcgccgttg
     6421 gtcgacttca cctcgaacac gccgtcgccg atctccagga tcgaga
//
LOCUS       DATNBV010000152         2275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2319500,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000152 DATNBV010000000
VERSION     DATNBV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2319500"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VN627_03600"
     CDS             complement(<1..660)
                     /locus_tag="VN627_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXD02371.1"
                     /translation="MTAALAPDATAAPKVGYPIYDADHHYYETPDAFLRHLDKKYHDK
                     FQYVTLANGRTKLVIDGFLSNYIPNPTFNVVAGPGTHENFYRANNPEGLTMREMGGKP
                     IKSIAAFHNGEAHLKMMDEQGIHAAIIYPTLASIIEVRLGHQVELTQALLHSLNLWVS
                     EEYGFGNGRQFPVGAISLSDPDWAVREMEFLLKAGCKQVLIRPAAVPTETGFRPWGDP
                     AL"
     gene            complement(820..1557)
                     /locus_tag="VN627_03605"
     CDS             complement(820..1557)
                     /locus_tag="VN627_03605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD02372.1"
                     /translation="MDPLFDFTGKVVLVTGGSRGLGKEMVLAFAERGADVIITSRKID
                     NCEAVADQVRAMGRRALAIGAHAAKWPEIDAMLDKAYAEFGRIDIAINNAGMSPAMPS
                     PDVTEALFDSVIGLNFKGPFRLAAVVGKRMMEGDGGVIINVSSSGAIIPLPGVVPYGA
                     SKAALNAMSRSLAKEFAPKVRVNVLSAGQFLTDIAKAWEPEQRETSNNIIGRPGRPEE
                     VVTAALALASPHSSYITGAFYQADGGL"
     gene            complement(1671..2093)
                     /locus_tag="VN627_03610"
     CDS             complement(1671..2093)
                     /locus_tag="VN627_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD02373.1"
                     /translation="MTPDQENLARMAIRHTQSIYNNCGDRGQVEEMVATFTPDGVMQT
                     NFGVYEGRDGILAFVRSVVARNGDADMAGSRHNLTTSRIEFESDTVALGWTYFFVSRR
                     GVTLEDGIYIDRYEKLGDRWLIAHRRVKSLWFLNPPES"
BASE COUNT          393 a          686 c          756 g          440 t
ORIGIN      
        1 gagcgcggga tcgccccaag gacggaagcc ggtttccgtc gggaccgccg ccgggcggat
       61 gaggacctgc ttgcagccgg ccttgagcag gaactccatc tcgcgcacgg cccagtccgg
      121 gtcggacagg ctgatcgcgc caaccgggaa ctggcggccg ttgccgaagc cgtattcctc
      181 ggaaacccac aggttcagcg agtgcagcag ggcctgggtc agctcgacct ggtgaccgag
      241 ccggacttcg atgatggacg ccagcgtcgg gtagatgatg gcggcatgga ttccctgctc
      301 gtccatcatc ttgaggtggg cctcgccatt gtggaaggcg gcgatcgact tgatcggctt
      361 gccgcccatc tcgcgcatgg tcagcccttc cgggttgttg gcgcgataga agttttcatg
      421 cgtgccagga ccggcaacca cgttgaaggt gggattgggg atgtagttcg agaggaaccc
      481 gtcgatcacc agcttggtgc ggccattggc gagggtcacg tactggaact tgtcgtgata
      541 tttcttgtcc aggtggcgca ggaaggcgtc gggcgtttcg tagtagtgat ggtcggcatc
      601 gtagatcggg tagccgacct tgggagcggc ggttgcatca ggggcgaggg cggcagtcat
      661 caggggcgat ctccacagag aaatgatgaa tcatcaaatt tcttctatgc ctgattttga
      721 tctgtgtcaa tttcctctgc accggggcgt ctccggacac gcaaacgggc gcggatctct
      781 cggatccgcg cccgctgaac gcctgcatcg ccgtcggggt cagagtccgc cgtcagcctg
      841 gtagaatgcg ccggtgatgt aggaggaatg cgggctggcg agggccaggg cggcggtcac
      901 cacttcctcc gggcggcccg gccgaccgat gatgttgttg ctggtttcgc gctgttcggg
      961 ctcccatgcc ttggcgatat ccgtgaggaa ctgcccggcc gagagcacgt tgacgcggac
     1021 cttcggcgcg aattccttgg cgagcgaacg gctcatggca ttgagcgcgg ccttggaggc
     1081 gccatagggc acgaccccgg ggagcgggat gatcgcgccg gaggaggaga cgttgatgat
     1141 caccccgcca tccccttcca tcatgcgctt gccgacgacg gcggcaaggc ggaacgggcc
     1201 cttgaagttc agcccgatga cgctgtcgaa cagggcttcg gtcacgtcgg ggctgggcat
     1261 ggccgggctc atcccggcat tgttgatggc gatatcgatc cgcccgaact cggcataggc
     1321 tttgtcgagc atggcatcga tttcgggcca cttggcggca tgggccccga tcgccagggc
     1381 gcggcggccc atggcgcgga cctggtcggc cacggcctcg caattgtcga tcttgcggct
     1441 ggtgatgatc acgtcagccc cgcgctcagc gaaagcgaga accatctcct tgcccaggcc
     1501 tcggctgccg ccggtgacga ggaccacctt gccggtgaaa tcgaacagcg gatccatgcc
     1561 agtcatctcc tcatatcccc caacgaacac agagttggcg acgccccgcc gcactcctgc
     1621 tggcggctgt tttgcatctc actagacact tgtccagtct gcatgtgatg tcaggattcg
     1681 ggaggattga ggaaccagag cgacttgacc ctgcggtggg caatcagcca gcggtcaccg
     1741 agcttctcgt aacggtcgat gtaaatccca tcctcgagag tcacgccgcg gcgcgagacg
     1801 aagaagtagg tccaccccag ggcgacggta tcgctctcga actcgatgcg cgaggtcgtc
     1861 agattgtggc ggctgccggc catgtccgca tcgccattgc gcgcaacaac cgaccggacg
     1921 aaggccagga tgccgtcgcg accctcgtag accccgaagt tggtctgcat gacaccatcc
     1981 ggcgtgaagg tggccaccat ctcctcgacc tgcccgcggt cgccgcagtt gttgtagatc
     2041 gattgggtgt ggcgaatggc catccgggcc aggttctctt gatcgggcgt catggtattc
     2101 ctctcgggcg atagcttgcg ccagattgcg gcgccccgca cgggtcggaa accatcctgc
     2161 aaatctatca tcagcttgat gtgctggccc tgcgcagcac ccttgcgatg gtatggccga
     2221 cggggacttg gcgaccccgc gccggtgcgg cactccttgg tgaaagcgtg tcgcc
//
LOCUS       DATNBV010000153         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_398923,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000153 DATNBV010000000
VERSION     DATNBV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398923"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VN627_03615"
     CDS             <1..307
                     /locus_tag="VN627_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02374.1"
                     /translation="DIVITCTASPLPIVGKGMVERAIRTRKHRPILMVDLAQTPDVEP
                     EVAELDDVFLFTAADLAAMVGDVAAHQAAHAQRQGHYGEAFSSPGGGCPPAHPGARA"
     gene            330..1688
                     /locus_tag="VN627_03620"
     CDS             330..1688
                     /locus_tag="VN627_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXD02375.1"
                     /translation="MNAELETGRDRRSMVRIIDPAMAAQDGERRAFLHRILHVGASVP
                     VLYALLDAEMAAAAGPPNATHELAIPPAPPAAALQTHPALVVRTFSDPYLELLRLLRE
                     ACEVEHALMMQYLYAAFSLKPAYHAIAGAAAPGSDTLLGVAIQEMEHLACVNRLLVAL
                     GAAPNLLPAEFPYEPDIYPFEFNLEPLTPKSLAKYVYVEAPIGYFDGQVRGADGRLIR
                     AVLDMIGRKRRPNHIGSLYAAIIALVREVGERPAMPDMKPWVAKLEAIKEEGEVHHFA
                     FFKSLFLASHPGFGGRANAWELAVPDANYPSYAMASNPTAYIGHENQVHNAKALALAW
                     LADLHYWTALLLLDQYFRNGDPAAHGLALAHMMGPLLALGRHLPKLGIGVPFDPLNLG
                     TSPGIDGQHNRRLITALLEEIVVVSKRVEDSLPADYAHEVTRQSLAAMREESPAFATL
                     AR"
     gene            complement(1739..>2079)
                     /locus_tag="VN627_03625"
     CDS             complement(1739..>2079)
                     /locus_tag="VN627_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HXD02376.1"
                     /translation="KKMFLAEIGQEEPGLNRLIRAAFKLLGLQTYFTAGVKEVRAWTI
                     HIGDSAPQAAGVIHTDFERGFIRAQTIAFDDYISYKGEQGAKDAGKMRSEGKEYVVKD
                     GDVLNFLFNV"
BASE COUNT          356 a          729 c          640 g          354 t
ORIGIN      
        1 cgacatcgtg atcacctgta cggccagccc gctgccgatc gtgggcaagg gcatggttga
       61 acgggccatc cggacccgca agcaccgccc gatcctcatg gtcgacctgg cgcagacgcc
      121 ggacgtcgag ccggaggtgg cggaactcga cgacgtcttc ctgttcacgg ccgccgacct
      181 cgccgcgatg gtgggcgatg tcgcggcgca tcaggcggcg catgcgcaac gacaggggca
      241 ttacggtgaa gccttcagca gccctggcgg gggctgcccg ccagcgcacc ccggcgcacg
      301 ggcgtagtcg atcaccgggg agaacgatcg tgaacgcaga acttgaaacc ggccgtgatc
      361 gccgcagcat ggtccgcatc atcgatccag cgatggcggc gcaagatggc gaaagacgcg
      421 cattcctgca ccgcatcctg catgtgggcg ccagtgtgcc ggtgctttac gccctgctcg
      481 acgccgaaat ggcggctgcc gccgggccac cgaacgcgac gcacgagctc gccattcctc
      541 ccgccccgcc ggcagcggcc ctccagacac acccggcact ggtggtgcgg actttctccg
      601 atccctacct cgagttgctc cgcctgctgc gcgaggcctg cgaggtcgag cacgcgctga
      661 tgatgcagta cctctatgcg gcgttttcac tcaagccggc ctaccacgca atcgccggcg
      721 ctgcggcgcc gggcagcgat accctgctcg gggtggcgat ccaggaaatg gaacacctgg
      781 cctgcgtcaa ccggctgctg gtcgcccttg gcgcggcgcc caacctgctg ccggccgagt
      841 ttccgtacga gcccgatatc taccccttcg agttcaatct cgaaccgctg acgccgaaga
      901 gcctcgccaa atacgtctat gtcgaggcac ccatcggcta cttcgatggc caggtgcgtg
      961 gcgccgatgg gcgcttgatc agggccgtcc tcgacatgat cggccgcaag cgccgcccga
     1021 accacatcgg cagcctctat gcggcgatca tcgccctcgt gcgcgaggtc ggcgagcggc
     1081 ccgcgatgcc cgacatgaag ccatgggtgg cgaagctgga agcgatcaag gaagagggcg
     1141 aggtgcatca ctttgcgttc ttcaagagcc tcttcctggc cagccatccg ggatttggcg
     1201 gccgcgccaa cgcctgggag cttgcggtcc ccgatgccaa ctatccgtcc tatgccatgg
     1261 ccagcaaccc gacggcctac atcggccacg aaaatcaggt gcacaacgcg aaggccctgg
     1321 cgctcgcctg gctggccgat ctccactact ggacggcgct gctgctgctc gatcaatact
     1381 tccgcaacgg cgaccccgcc gcccacggcc tcgcgctcgc ccacatgatg gggccgctgc
     1441 ttgcgcttgg acggcacttg cccaagctgg gcatcggtgt ccccttcgac ccgttaaacc
     1501 ttggcaccag ccccggcatc gacggtcagc acaaccggcg gctgatcacc gcgctgctcg
     1561 aggagatcgt cgtcgtcagc aaacgcgtcg aagactcgct gccggccgac tatgcccatg
     1621 aggtgacccg gcagtccctc gccgcaatgc gcgaggaaag cccggcattc gccaccctgg
     1681 cccggtaagt tgcccgatcc gatcccggca cggcatgcgc ctggatccag gatctggttc
     1741 acacgttgaa caggaagttc agcacatcgc cgtccttgac cacgtattcc ttgccctcgc
     1801 tgcgcatctt gcccgcgtcc ttggcgccct gctcaccctt gtacgaaatg tagtcatcga
     1861 atgcgatcgt ctgggcgcgg atgaagccgc gctcgaagtc ggtgtgaata actccggcag
     1921 cttgcggtgc gctgtcgccg atgtgaatcg tccaggcccg cacctccttc acgccggccg
     1981 tgaagtaggt ctgcaggccc agcagcttga aggccgcgcg gatcaggcga ttcaggccgg
     2041 gctcctcctg cccgatttcg gcgaggaaca tcttcttgt
//
LOCUS       DATNBV010000154         2312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_111319,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000154 DATNBV010000000
VERSION     DATNBV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111319"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VN627_03630"
     CDS             complement(<1..1152)
                     /locus_tag="VN627_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXD02377.1"
                     /translation="MSPRNYPSIVVPPPGPKAQAVVARDAEWTSTCYIKEYPLVVARG
                     SGVMVEDVDGNRYLDFMAGIAVSSTGYGHPKVVAAVKEAADRFLHICGSDFYYEGMAN
                     LCERLARVAPGPSKKRVFLTNSGTEATEAAIKLARYATGRTAISAFRGAFHGRTTGAV
                     TLTSSKARQHAGFGPLLPDVHHVPYAYRYRCQFCGDEPACNRGCIGVIEEDLFARHLD
                     PHDVAAIFIEPIQGEGGYVVPPPGYLADLRALCDRYGILLVADEVQSGVGRTGKMFAC
                     EHEGVEPDILLTAKGLGSGMPIGAMISRESITTWESGSHGSTFGGNPVCCAAALATLD
                     LVEGGLMANAARLGERLMGGACRLMNKHSCIGDVRGRGLMVGMEFVKDRT"
     gene            complement(1164..>2312)
                     /locus_tag="VN627_03635"
     CDS             complement(1164..>2312)
                     /locus_tag="VN627_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXD02378.1"
                     /translation="DLLDYYAKQVQDADGYVKPLGKLLPNENTRSVMRPYGVFACIAP
                     FNFPLALSTGMSAAALLTGNTVVYKPSEDTPWTGLRLAEIYRDAGLPAGVFNFIPGRR
                     EEIGDAMWQHPGVDGVVFTGSKAVGLRIHAGLSGGWIKPALLELGGKNAAIVLESADL
                     DAAAEGVMRSAFSLQNQKCSATSRVYVQREVAAPFIQRLVEKTRGIRMGDPSERDIFF
                     GPVINQQAVDRFERAVSQARTEGSIVLGGERLLGGPFDRGYFVAPTIARLPLESTLYR
                     DELFVPFVAIGEVSGLAQALQETNRVEYGLTAGIFSKNPAEVARFFDEVEAGVCYANK
                     RTGATTGAWPGAQPFCGWKASGSTGKGGCGPYYVAQFMREQSRTVIEG"
BASE COUNT          386 a          811 c          717 g          398 t
ORIGIN      
        1 ggtccgatcc ttgacgaact ccattcccac catcaacccg cggccccgca catcgccgat
       61 gcaggagtgc ttgttcatca gccggcaggc tccgcccatc agccgctcgc ccagacgcgc
      121 ggcgttggcc atcagcccgc cttccaccag gtccagtgtg gccagcgcgg cggcgcagca
      181 aaccgggttc ccgccgaatg tggagccatg cgacccgctc tcccaggtgg tgatcgactc
      241 tcgggagatc atggcgccga tcggcatgcc cgagcccagt cccttggccg tgagcaggat
      301 atcgggctcg acgccctcgt gctcgcaggc gaacatcttt ccggtccggc ccacacccga
      361 ctggacctcg tcggccacga gcagaattcc gtagcggtcg cacagcgccc gaagatcagc
      421 caggtagccg ggaggcggca ccacgtagcc accctctccc tgaatcggct cgatgaagat
      481 ggccgcgacg tcgtggggat cgaggtggcg ggcgaacagg tcctcttcga tcaccccgat
      541 gcatccgcga ttgcaagcgg gctcgtcgcc gcagaactga caacggtagc ggtacgcgta
      601 cggcacgtgg tgaacgtcgg gcagcagcgg accgaaaccc gcatgctgtc gcgccttgct
      661 cgacgtaagc gtcactgctc cggtggtccg cccgtgaaat gcgccgcgga acgcgctgat
      721 ggcagttctc cccgtggcgt agcgggccag cttgatcgct gcctccgttg cttccgttcc
      781 cgagttggtg agaaacactc gctttttgga cggccccggc gccactctgg ccagccgctc
      841 acagaggttg gccatcccct cgtagtagaa atcggacccg cagatgtgca ggaaccggtc
      901 ggccgcctcc ttcaccgccg cgaccacctt gggatggccg tacccggtgg aggacaccgc
      961 gatgcccgcc atgaagtcga gatagcggtt gccgtccaca tcctccacca tcacaccgct
     1021 gccccgcgcg acgaccagcg ggtactcctt gatgtagcag gtggaggtcc attccgcgtc
     1081 gcgcgccacc acggcctgcg ccttggggcc ggggggcggc acgacgatgc tggggtagtt
     1141 gcgcgggctc atgaagtgtt tccttatccc tctatgaccg ttcggctctg ctctcgcatg
     1201 aattgcgcga cgtaataggg gccgcatccg cccttcccgg tggagcccga tgccttccag
     1261 ccgcaaaacg gctgggcgcc gggccaggcc cccgttgtcg caccggtccg cttgttggcg
     1321 taacacaccc cggcctccac ctcatcgaag aaccgggcca cctccgcagg attcttggag
     1381 aaaatccctg ccgtgagtcc gtattctacc cggttggtct cctggagcgc ctgggccaga
     1441 ccactcacct cgccgatcgc gacaaacggc acaaatagct cgtcccggta gagcgtgctc
     1501 tccagcggca atcgcgcaat cgtgggagca acgaagtacc ctcgatcgaa cgggccaccc
     1561 aggagccgct cccctcccag cacgatgctg ccctcggttc gtgcctggga taccgcgcgc
     1621 tcgaaccgat ccactgcctg ctggttgatc accgggccga agaagatgtc tcgctccgag
     1681 ggatcgccca tccggatgcc gcgggtcttc tccaccagcc gctggatgaa gggcgcggcc
     1741 acctcgcgct gaacgtacac ccgcgatgtc gcgctgcact tctggttctg caggctgaac
     1801 gccgagcgca tcacgccttc ggccgcggcg tccagatcgg cgctctccag cacgatggcc
     1861 gcgttctttc cgcccagctc cagcagtgcg ggcttgatcc agccgccgct gagcccggca
     1921 tggattctga gccctacagc ctttgatccg gtgaacacca cgccgtccac gccggggtgc
     1981 tgccacatcg cgtcgccgat ttcttcccgg cgacccggaa tgaagttgaa gacacccgcc
     2041 ggcaggccgg catcccgata gatctccgcc aggcgaagcc cggtccacgg ggtgtcttcc
     2101 gagggcttgt acacgacggt gttgcccgtg aggagcgctg cggccgacat accggtggag
     2161 agggcgagcg ggaaattgaa cggcgcgatg caggcgaaga cgccgtaagg ccgcatcacc
     2221 gagcgcgtgt tctcgttggg gaggagcttg cccagcggct tcacgtagcc gtcggcgtcc
     2281 tgcacctgct tggcgtagta gtcgagcagg tc
//
LOCUS       DATNBV010000155         3649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1065259,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000155 DATNBV010000000
VERSION     DATNBV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065259"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..997)
                     /locus_tag="VN627_03640"
     CDS             complement(302..997)
                     /locus_tag="VN627_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HXD02379.1"
                     /translation="MSWTDERIEKLTKMWEGGSTASQIADELGGVSRNAVIGKAHRLG
                     LKARPSPVKPNEKEAAPAAAPRPAREQAAPAAPRPAPAPKAEAAPRPAPSASAPRPAA
                     PAPVAAAPASGEPAAPPPPRIVSVGPGGFLRQGPGDQQAPIPPAPPRRLVPAKPSPEI
                     ADKTSLLDLNDRICRWPMGHPGEPDFHFCGDKVNPGFPYCVEHCGRAYQAQLPRGARR
                     APPPLPFGGPRVR"
     gene            1089..1961
                     /locus_tag="VN627_03645"
     CDS             1089..1961
                     /locus_tag="VN627_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXD02380.1"
                     /translation="MADQPQLAAASDAPVPARDFPAQGVPIIHGINGLGLWTLYIKEV
                     RRFFKVQTQTIWAPAITTLLYLMIFTVALGGGSRLVLGVPFATFVAPGLIAMGMMNNA
                     FANSSFSFLAGKIQGTIVDYLMPPLSEMELMIALVGAALTRAVLVGLALAAAIWLWPG
                     VDLTVHHWWAVIWFGLMGSVLLALIGLLTSLWAEKFDHNAAVTNFVITPLTMLSGTFY
                     VIDRLAPAFQTVSRMNPFFYVISGFRFGFLGQSDIGSTNMAVLHGGIGLGVLNLVLGV
                     VTYALLKSGWRLKN"
     gene            complement(2054..2434)
                     /gene="hspQ"
                     /locus_tag="VN627_03650"
     CDS             complement(2054..2434)
                     /gene="hspQ"
                     /locus_tag="VN627_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HXD02381.1"
                     /translation="MERASFFSPQAGRLIDAPRHAHARFGIGDVVRHKSADFRGVIFD
                     IDPVFANSEEWYEAIPQEVRPTREQPYYHLLAENGENAYVAYVSQQNLVGDGESGPVE
                     HPSVEQLFEDFRDGHYRLRRGLTH"
     gene            complement(2506..3522)
                     /gene="virB11"
                     /locus_tag="VN627_03655"
     CDS             complement(2506..3522)
                     /gene="virB11"
                     /locus_tag="VN627_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HXD02382.1"
                     /translation="MLPLPPSEVLPHGEPLHLPRSVYLDAYLAPFRPWLECETVTEIL
                     VNRPGELWIEDAATPGMTRIDLPEVTDELLLRLAQQVARVSHQGINREHPLLAATLPD
                     GARVQLCGPPATREHWAMAIRRHRLLDLGLDAYDRGPLTPRAEAPWPDPQAQPIAYLR
                     EAVRRRRTILVSGGTSTGKTTFLNAMLREIPAEDRVVLVEDTAELRLPGTNGVGLIAV
                     HGELGEARVSADELVQAALRLRPDRIVVGELRGAETISFLRAINTGHPGSFSTVHANS
                     PRGALDQIALMAMQTGIGLTRTETLDYAASVIDLVVQLDRVDGRRGVAAIASTAALRD
                     CVQA"
     gene            complement(3534..>3649)
                     /locus_tag="VN627_03660"
     CDS             complement(3534..>3649)
                     /locus_tag="VN627_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HXD02383.1"
                     /translation="AAVGQTGQIGPTVSVRQGQPIRVFTARDLDFSKVTAP"
BASE COUNT          512 a         1110 c         1401 g          626 t
ORIGIN      
        1 gccttggggg cgggagccgg gcgcggcgcg gccggggcag cctgttcgcg cgccggcctg
       61 ggcgcgggtg gaagccgggg tcgattcact cgggatcaag ggcctgatgc tctcggtctg
      121 gggtgacgcg ggcaagacca agtcgatcgg cggccgcgtc ggattccgat tctgatcgat
      181 tgccgcaagg ctaacaggaa ggggcgggcc gcgaggtccg cccctttcgc ttgggcgccg
      241 ggcgcaggcg aaaaaaggcc cgcggggttc cgcgggcctt tgttgtgctt gggttagaaa
      301 cttagcggac gcgtgggccg ccgaagggga gcggcggcgg cgcgcggcgg gcgccgcgcg
      361 ggagctgggc ctggtaggcc cggccgcagt gttcgacaca gtaggggaac ccggggttca
      421 ccttgtcacc acagaagtgg aaatcgggct cgcccgggtg gcccatcggc cagcggcaga
      481 tccggtcgtt cagatcgagc aggctggtct tgtcggcgat ttccgggctg ggcttggcgg
      541 gaaccaggcg gcgcggcggg gccggaggga tcggggcctg ctggtcaccc ggtccctggc
      601 ggaggaaccc gcccgggccg accgagacga tccgcggtgg cggcggtgca gcgggttcgc
      661 cggaagccgg cgcggcggcg accggagcgg gtgctgccgg gcgcggggcc gaggcggagg
      721 gcgccgggcg cggggcggct tcggccttgg gggcgggagc cgggcgcggc gcggccgggg
      781 cagcctgttc gcgcgccggc ctgggcgcgg cggccggggc agcctccttc tcgttgggct
      841 tgaccgggga gggccgggcc ttgagcccaa gccggtgggc cttgccgatc accgcgttgc
      901 ggctgacgcc gcccagttca tcggcgatct ggctggcggt tgatccgcct tcccacatct
      961 tggtcagctt ttcgatgcgt tcgtcggtcc agctcatgcc tgacgtgtac tttccttcgc
     1021 ggcgcccgcc gggcccaatg ccccgggtgc gcttgccaat ggcttgcggt gccgatagtc
     1081 gccactgcat ggccgatcaa ccccagcttg ccgccgcgtc cgatgcacct gttccggcac
     1141 gggattttcc cgcgcagggg gtgccgatca ttcacgggat caacgggctg ggcctgtgga
     1201 ccctctatat caaggaagtg cgtcgctttt tcaaggttca gacccagacg atctgggccc
     1261 cggcgatcac gaccctgctt tacctgatga tcttcaccgt agcgctgggt ggcggaagcc
     1321 ggctggtgct gggcgtgccc ttcgccacgt tcgttgcgcc ggggctgatc gcgatgggca
     1381 tgatgaacaa cgccttcgcc aattcgagct tcagtttcct ggccggcaag atccagggga
     1441 ccatcgtcga ctacctgatg ccgccgcttt cggagatgga gctgatgatc gcgctggtcg
     1501 gcgcggcgct gacccgggcc gtgctggtgg gcctggcgct tgccgcggcg atctggctgt
     1561 ggcccggggt ggaccttacg gtgcaccact ggtgggcggt gatctggttc gggctgatgg
     1621 gctcggtcct cctcgcgctg atcggcctgc tgacctcgct gtgggccgag aagttcgatc
     1681 acaacgcggc ggtgaccaac ttcgtgatca cgccgctgac catgctttcg ggcacgttct
     1741 acgtgatcga ccggctggcc ccggctttcc agaccgtcag ccggatgaac ccgttcttct
     1801 acgtgatctc gggcttccgc ttcggcttcc tcggccagag cgacatcggc agcacgaaca
     1861 tggccgtgct gcatggcggg atcgggctgg gcgtgctcaa cctcgtgctg ggcgtggtga
     1921 cctatgccct gctgaagagc ggctggcggc tgaagaattg attggtgcgg gggagaagct
     1981 cctcgctggc gctcggcgta ggccttcgac ttcgctcggg agcgagtggg gaaataagag
     2041 gacgttattg aacctagtgc gtcagcccgc ggcgcaggcg gtagtgtccg tcgcggaaat
     2101 cctcgaacag ctgttcgacc gaggggtgct cgaccggccc gctttcgcca tcgccgacca
     2161 ggttctgctg gctgacatag gcgacatagg cattttcgcc attctcggcg aggaggtggt
     2221 agtagggctg ttcgcgcgtg gggcggactt cctgcgggat ggcttcgtac cattcctcgc
     2281 tgttggcgaa gaccggatcg atatcgaaga tcacgccgcg aaagtcagcc gacttgtggc
     2341 gcaccacgtc gccgatgccg aagcgggcgt gggcatggcg cggcgcatcg atcagcctgc
     2401 cggcctgggg agagaagaag ctggcgcgtt cgatccgttg cataagccca atataggccg
     2461 attgcccggg gcggcaaggc gacggcccgg cgatcccccg atttctcagg cctggacaca
     2521 atcgcgcagg gcggccgtgg aggcaatggc ggcgactccg cggcggccat ccacccggtc
     2581 gagctggacc acgaggtcga tcaccgaggc ggcatagtcg agcgtttcgg tgcgggtgag
     2641 gccgataccg gtctgcatgg ccatcagcgc gatctgatcg agcgcgccgc gtggggaatt
     2701 ggcgtggacg gtggagaagg agccggggtg gccggtgttg atggcgcgca ggaaactgat
     2761 cgtttcggcc ccgcgcagtt cgcccaccac gatccggtcg ggtcgcaggc gcagcgcggc
     2821 ctggaccagt tcgtcggccg agacgcgcgc ttcgcccagc tcgccgtgca cggcgataag
     2881 gccgacgccg ttggttccgg gcaggcgcag ctcggcggtg tcttccacca gcacgacgcg
     2941 atcttcggcg gggatttcgc gcagcatggc gttgaggaag gtggtcttgc cggtggaggt
     3001 gccgcccgag acgaggatcg tgcgccgccg ccgcacggct tcgcgcaggt aggcgatggg
     3061 ctgggcctgg ggatcgggcc agggggcttc ggcgcgcggg gtgagcgggc cgcgatcata
     3121 tgcatcaagg ccgagatcga gcaggcgatg gcggcggatg gccatggccc aatgctcgcg
     3181 cgtggccggc gggccgcaca gttggacgcg ggcgccatcg ggcagggtgg cggcgagcag
     3241 ggggtgttcg cggttgatcc cctggtggct gacccgggcg acttgctggg cgaggcgcag
     3301 gagcagctcg tcggtgactt cgggcagatc gatccgggtc atacccggcg tggcggcatc
     3361 ctcgatccac agctcaccgg gccggttgac gaggatttcc gtgacggtct cgcactccag
     3421 ccagggtcgg aagggggcga gataggcatc gagatagacg ctgcggggca agtgcagcgg
     3481 ctcgccatgg ggcaggactt cggacggggg caagggcagc aggccgcctt cgctcacggc
     3541 gcggtgactt tcgaaaaatc gagatcgcgc gcggtgaaga cgcggatcgg ctggccctgg
     3601 cggacgctca cggtcggccc gatctgaccg gtctggccga cggctgccg
//
LOCUS       DATNBV010000156         4273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_737935,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000156 DATNBV010000000
VERSION     DATNBV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737935"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VN627_03665"
     CDS             <1..953
                     /locus_tag="VN627_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HXD02384.1"
                     /translation="FEEHCEVLLTSQGEARQIDADILAKCLLQARSKNRLAEGMGKIT
                     VDSPIPYLLSDLSNILQNEMGKLDKGTNSIPYMRIKTKLDELKGDPRYQFMFSGVLVG
                     DTMAEFISKIFRLPASGKPISIVDVSGVPSEVTSTVVAVLGRLIFDFAIWARDEETRP
                     VLLVCEEAHRYVPNERNADGSSVGRVLSRIAKEGRKYGVSLGLITQRPSDLAEGVLSQ
                     CGTIISMRLNNDRDQAFVKAAMPEGARGFLDSIPALRNRECIICGEGVSVPIRVSFDD
                     LEEIKRPASADPSISELWRQSGGEEEMVQRTVMRWRAQGR"
     gene            complement(1035..2492)
                     /locus_tag="VN627_03670"
     CDS             complement(1035..2492)
                     /locus_tag="VN627_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXD02385.1"
                     /translation="MNLPAPASSSPSSSSPADTTDQPGAASPRPLHGDLTQGPILRTL
                     LVFSIPTLLSNMLQTLNGSVNAVWVGRLIGESALAATANANVIMFLLFSAVFGFSMAT
                     TVKVGQHFGARNIAASRQTFGAGTGFCVMLSLVVAVFGWVASPWLLHLMATPPESMTE
                     ADAYLRIIFLSIPFSTASLMLAMGLRGVGDARTPLYAMVLTVAVDIVLNPLLIRGAGP
                     LPAFGIAGSAASTAVANLTGALLMLWALYARDLPLRLRGRELAHLWPARSELGYIITK
                     GLPMGAQMLLISSAQLVILGLVNREGLDTTAAYGASLQLWNYLQMPAFAIASAVSAMV
                     AQAIGAGYHERVGQVTRVGVIGNLALTGVLAAIIVAAHEPLFALFLGADSPAIPIAGH
                     IQAICTWSFMLSGMMMVLTGTMRSYGAVISPLVIMFVAFYPARLGFYFLAHPIMGGEA
                     LWWSYPFSSAASVLMTWWAYTRGSWRKQRDRAYAP"
     gene            complement(2498..3934)
                     /locus_tag="VN627_03675"
     CDS             complement(2498..3934)
                     /locus_tag="VN627_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXD02386.1"
                     /translation="MTMLARQVEAAPTASGSLATGPILRTMLAFSASTLVSNLLQTVI
                     QSIGMIWIGRLLGEEALAATANGNQVMSLLSTVMLGLAMATMIRIGRHAGAGDASEAR
                     GAFACGLGLTIALGLALGLGGFLLTDRILHLLGTPPESLAEARAYLRVMFLTLPAIGL
                     TNTFAMASRGAGNARIPLYGTVVTLLASATLTPVLILGFAGLPRLGVAGAALASGLSN
                     LLGVLLMAALLWRRSSPLRLGRAELPRLAPWDRESPWLLRNGVPISVHMNLVVLAQLV
                     MIGLVNREGLNYTAAFGVSLQIWLYLQMPSFAISAGITAMAAQAIGAGDHVRVGQIKR
                     TGVRVSLTIVGLMIAVILLLIRPILALFLGAGSPALPIGVHMQVICIWAFIFTTAMSA
                     MTAVLRAYGAQLVTVVVNLVALFGMRLACYFAAYPMIGADALWWSFVVGSAASLALTH
                     LAYQRGGWGREAEPGLRRRLAEGDHAPS"
     gene            complement(3931..>4273)
                     /locus_tag="VN627_03680"
     CDS             complement(3931..>4273)
                     /locus_tag="VN627_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HXD02387.1"
                     /translation="LDPLVSQYNVSPKLAHSGNPAELALVPERLAAFAADPRAWFKFV
                     IASPGDLAEVLALRDAHHMAAERILLMPEGTSPATLRERMAWLAPLALEHGLRLTDRL
                     HIHLYGDTRGT"
BASE COUNT          766 a         1359 c         1554 g          594 t
ORIGIN      
        1 acttcgagga gcactgcgag gtgctgctga cctcgcaggg cgaagcgcgc cagatcgatg
       61 ccgacatcct tgccaagtgc ctgctccagg cgcggtccaa gaaccgcctc gccgagggca
      121 tgggcaagat cacggtcgac agtccgattc cctacctgct ttcggacctg tccaacatcc
      181 tccagaacga gatgggcaag ctcgacaagg gcaccaactc gatcccctac atgcggatca
      241 agaccaagct cgacgagctc aagggcgatc cgcgctacca gttcatgttc tcgggcgtgc
      301 tggtgggtga caccatggcc gagttcatct ccaagatctt ccgcctgccg gcctcgggca
      361 agccgatctc gatcgttgat gtctcgggcg tgccctcgga agtgacctcg accgtggttg
      421 ccgtgctggg ccggctgatc ttcgactttg cgatctgggc ccgcgacgag gagacgcggc
      481 ccgtcctcct cgtctgtgag gaagcccacc gctacgtccc caacgagcgc aatgccgatg
      541 gctcctcggt tggccgcgtc ctatcccgca tcgccaagga aggccgcaaa tacggcgttt
      601 ccctgggcct gatcacccag cgcccctcgg accttgccga gggcgtgctc tcgcagtgcg
      661 gcacgatcat ctcgatgcgc ctcaacaacg accgcgacca ggcctttgtg aaagcggcca
      721 tgccggaagg cgcgcgcggc ttcctcgatt cgattccggc cctgcgcaac cgcgaatgca
      781 tcatctgcgg cgaaggtgtc tcggtgccga tccgcgtttc gttcgacgat ctcgaagaga
      841 tcaagcgccc ggcctcggcc gacccctcga tctccgagct gtggcgccag tccggcggcg
      901 aggaggagat ggtgcagcgc accgtgatgc gctggcgcgc gcaggggcgg taaatccggc
      961 ctgcagcggc ccgcggcggc ctgcgaacgg catctttctc ggctcaccct gggctggctg
     1021 cggccagatt gcggctacgg cgcgtaggcc ctgtcgcgct gcttgcgcca ggacccgcgg
     1081 gtgtaggccc accaggtcat cagcaccgat gcggccgagc tgaacggata ggaccaccac
     1141 agcgcctcgc cgcccatgat cggatgagcc aggaagtaga agccgagccg cgccgggtag
     1201 aaggccacga acatgatcac gagcggtgaa atcacggcgc catagctgcg catggtcccg
     1261 gtcagcacca tcatcatgcc cgagagcatg aacgaccagg tacagatcgc ctgaatgtgg
     1321 ccggcgatgg gaatggccgg actgtcggcc cccaggaaca gcgcgaacag cggttcgtgc
     1381 gcggcgacga tgatcgcggc cagcacgccg gtcagggcga ggttgccgat cacgccgacc
     1441 cgggtgacct ggcccacccg ctcgtgatag cccgcgccga ttgcctgggc gaccatggcg
     1501 ctcaccgccg aggcgatggc gaatgccggc atctggaggt agttccacag ctggagcgag
     1561 gcgccgtagg ccgccgtggt gtccagcccc tcgcggttga cgaggccgag gatcaccagc
     1621 tgggccgagg agatcagcag catctgcgcc cccattggca ggcccttggt gatgatatag
     1681 ccgagttccg accgggccgg ccacaggtgc gccagctcgc ggcccctgag gcgcagcggc
     1741 aggtcgcggg catagagggc ccagagcatc agcaatgcgc cggtgaggtt ggcaacggcg
     1801 gtggaggccg ccgagccggc gatgccgaag gccggcagcg ggccggcgcc gcggatcagc
     1861 agcgggttca gcacgatgtc gaccgccacg gtcagcacca tcgcatagag cggagtgcgg
     1921 gcatcgccca ccccgcgcag gcccatggcg agcatcagcg aggcggtcga gaaggggatc
     1981 gagaggaaga tgatgcgcag gtaggcgtcg gcttcggtca tcgactcggg gggcgtcgcc
     2041 atcaggtgga gcagccaggg cgaggccacc cacccgaaca ccgccacgac cagcgaaagc
     2101 atgacgcaga agcccgtgcc cgcgccgaag gtctgccgcg aggcggcgat gttgcgggcg
     2161 ccgaaatgct ggccgacctt gacagtggtc gccatcgaga agccgaacac ggccgagaac
     2221 agcaggaaca tgatcacgtt ggcattggcc gtcgccgcca gcgcgctctc gccgatcagc
     2281 cggccgaccc agacggcgtt gaccgagccg ttgagggtct ggagcatgtt cgagaggagg
     2341 gtggggatcg agaacaccag cagggtgcgc aggatcggcc cctgggtgag atcaccatgc
     2401 agcgggcgcg gcgatgcggc gccgggttgg tcggtcgtgt cagctgggga ggaggaggag
     2461 ggggaggacg aggcaggtgc gggcaagttc atttccgtca agatggggca tgatcgccct
     2521 ccgccaagcg gcggcgcagg ccgggctcgg cctcgcgccc ccagccgcct cgctggtagg
     2581 caaggtgggt gagcgccagt gaagcagccg agccgaccac gaacgaccac cacagggcgt
     2641 cggctccgat catcggatag gcggcgaagt agcacgccag ccgcatgccg aacagggcga
     2701 cgaggttgac cactacggtt accagctgcg cgccgtaggc ccggagcacc gcggtcatcg
     2761 cgctcatcgc cgtggtgaag atgaacgccc agatgcagat gacctgcatg tgcacgccga
     2821 tcggcagggc cgggctgccc gcgccgagga acagggccag gatcgggcgg atgagcagca
     2881 ggatcacggc gatcatcagg ccgacgatgg taagcgagac gcgcactccg gtgcgcttga
     2941 tctgccccac ccggacatga tcgcccgcgc cgatggcctg ggctgccatg gcggtgattc
     3001 cggcgctgat cgcgaagctg ggcatctgca ggtagagcca gatctgcagg ctgaccccga
     3061 aggcggcggt gtaattgagc ccttcgcgat tgacgaggcc gatcatcacc agctgggcga
     3121 ggaccacgag gttcatgtgc accgaaatcg gcacgccgtt gcgcagcagc cacggggatt
     3181 cgcggtccca cggggccagc cggggcagct cggcccggcc caggcgcagc ggcgaggagc
     3241 ggcgccagag cagggcggcc atgaggagga cgccgagcag gttggacagc ccgctcgcca
     3301 gcgccgcgcc ggcaacgccc aggcgaggca ggccggcgaa gccgaggatc aggaccgggg
     3361 tcagcgtggc cgaggcgagg agggtgacga ccgttccgta gagcgggatg cgcgcgtttc
     3421 cggcaccgcg cgaggccatg gcgaaggtgt tggtgaggcc tatcgccggc agggtcagga
     3481 acatgacgcg caggtaggcc cgcgcctcgg cgaggctttc gggcggcgtg ccgagcaggt
     3541 gcaggatccg gtcggtcagc aggaagccgc cgaggccgag ggcgaggccc agggcgatgg
     3601 tgagccccag gccacaggcg aaggcgccgc gcgcctcgga cgcatcgccc gcgccggcgt
     3661 ggcggccgat gcggatcatc gtggccatgg cgaggccgag catgaccgtg ctgagcaagc
     3721 tcatcacctg gttgccgttg gccgtggcgg cgagtgcttc ctcgcccagc aggcggccga
     3781 tccagatcat gccgatcgac tggatgacgg tctgcaacag gttggagaca agggtcgagg
     3841 ccgagaaggc cagcatggtg cgcaggatcg ggcccgtggc gagcgaaccg gaagcggtag
     3901 gcgccgcttc cacctgccgg gccagcatgg tcatgtgccg cgcgtgtcgc cgtagaggtg
     3961 gatatggagc cggtcggtca gccttaggcc atgttcaagc gccagggggg cgagccaggc
     4021 catccgctca cgcagggtgg cggggctagt cccttcgggc atgagcagga tgcgttcggc
     4081 ggccatgtgg tgagcgtcgc gcagggcgag gacctccgca aggtcgccag gcgaagcgat
     4141 cacgaacttg aaccaggcgc ggggatcggc ggcgaaggca gcaaggcgtt cgggcacgag
     4201 cgccagttct gccgggttgc cggaatgggc gagcttgggg ctgacgttgt actggctgac
     4261 cagcggatcg agg
//
LOCUS       DATNBV010000157         2987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_738251,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000157 DATNBV010000000
VERSION     DATNBV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738251"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VN627_03685"
     CDS             <1..747
                     /locus_tag="VN627_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD02388.1"
                     /translation="AIAEALAKEGAEVIIHGRDPTSAEAVRDRIVAAGGRAHIVLASL
                     SDPAAVDDLARQALALGPIDILVNSAGAAPTINHWFTGPDDAWQQQFQLSTFYVAQLI
                     RAIVPAMRARGWGRVINISSGAAIRGMPLHPEYAAAKLALHSMTATLAAELGDCGVTV
                     NAIVAGGVATPNTLKNISENARLHGFTETGLAQEKRVIAEVWKAQIPLARLGRPEEVA
                     DAACFLASARASYITGATLRVDGGASGAVF"
     gene            952..2277
                     /locus_tag="VN627_03690"
     CDS             952..2277
                     /locus_tag="VN627_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HXD02389.1"
                     /translation="MGKLTTTKVRTAKPGVTPEGMPVKAVYQDGEGLFLLVKPSGARF
                     WMLRVQANGRRRDIGLGAVALEGVGAGAFGDHDPVADTSLMLRSSLTLAEARRKAAAL
                     RELAKAGTDPVAARDRERVKIPTFAEAVTEAHKALASGWSPKTAKAFKASLEEHATPK
                     LGALLVNAIGTAEVITALAPIWTDKPVMARKVRTRIGQVLAFAKARGWRKDALPDARE
                     LRSGLSKQGRGGNFAAMPYAELPGFVAGELGREATASRTAMLFAVLTGARSGEVRNAT
                     WEQIDLDRGTWTRPAAIMKMKEGHVVTLSAAAIALLERYTPNDLRKGLVFSGVRGGPL
                     SDMSLTKALRTAGRGETVHGFRSAFRDWAAEQMPTIPAMVAEMALAHKVGTKTEQAYL
                     RSDLRDMRRALMDAWGRFAAPSLSPGGSNVVDMSRTGQASQQRAAGGQS"
     gene            2274..2444
                     /locus_tag="VN627_03695"
     CDS             2274..2444
                     /locus_tag="VN627_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02390.1"
                     /translation="MSDLEFIVLFVAIYGIGIAAAWFADRYEVHVSAIREEEYLRGFH
                     DGRFTKKPPPHM"
     gene            2629..2937
                     /locus_tag="VN627_03700"
     CDS             2629..2937
                     /locus_tag="VN627_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXD02391.1"
                     /translation="MTAATMRTNLGDWENPPVIQDQVIAYMEQMGGAMILSDIYRPLG
                     LPRYTVNRVLGRLHSKGVLSRYKIPVRLRQYDRCQGGALPDGATRQCYLYSFADEVVA
                     "
BASE COUNT          545 a          914 c         1028 g          500 t
ORIGIN      
        1 gccatcgccg aagcgctcgc caaggagggg gccgaggtca tcatccacgg ccgggatccg
       61 acaagcgccg aggccgttcg cgaccggatc gtggcggcgg gcggaagggc gcatatcgtg
      121 ctcgcatccc tgtccgatcc ggctgcggtc gacgatctgg cgcggcaggc gctggcgctg
      181 ggcccgatcg acattctcgt caacagcgcc ggcgctgcgc cgacaatcaa tcactggttt
      241 acaggtccgg acgatgcctg gcagcagcag ttccagcttt cgaccttcta cgtggcccag
      301 ctgatccgtg ccatcgttcc ggcgatgcgc gcgcggggat ggggccgggt gatcaacatc
      361 agcagcggcg ccgcgatcag gggcatgccg cttcacccgg aatatgcggc ggccaagctg
      421 gcgctccatt cgatgacggc aacactcgcg gcggagctgg gggactgcgg ggtgaccgtg
      481 aatgccatcg tcgccggcgg cgtcgccacg cccaacacgc tgaaaaatat cagcgagaat
      541 gcccgccttc acggctttac cgaaaccggt ctggcacagg aaaagcgggt gatcgcggaa
      601 gtgtggaagg cgcagattcc gctcgcccgg ctgggccgtc ccgaagaggt ggccgatgcc
      661 gcctgcttcc tcgcatcggc gcgcgcgtcc tacatcactg gtgccacgct gcgggttgac
      721 ggcggcgcga gcggggccgt cttctgaggc ccgccgggcc tggcgctggc gatgaaggaa
      781 aatggctccc tgagtaggat tataactcgc gtccagcagg gggcatcgat ttctaccgat
      841 gtccacgtaa cccgcataaa gccgttgcat tccgtctacg ctcgtccatg attggaaatc
      901 ggcactgacc cacagccaag gaattgtgtg gggaccgatg tggggactgc aatgggcaag
      961 ctgacaacga ccaaggtgcg aacggccaag ccgggggtga cgccagaggg catgccggtg
     1021 aaggcggtct atcaggacgg agagggcttg ttcctcttgg tgaagccttc gggcgcgcgt
     1081 ttctggatgc tccgggtgca ggcaaacggc aggcgccgcg acatcggcct tggcgccgtc
     1141 gcgcttgagg gcgtcggggc tggtgcattc ggcgaccatg atccggttgc cgacacctcg
     1201 cttatgctac ggtcgtcgct cacgctggcc gaggcgcgca ggaaggcggc tgcgctgcgc
     1261 gagctggcga aggctggaac ggaccccgtg gccgcacgag acagggagcg ggtcaaaatc
     1321 ccgaccttcg ccgaggcggt gacagaggcg cacaaggccc tagcgtccgg ctggagcccg
     1381 aagacggcca aggcgttcaa ggcctcgctg gaggaacatg ccaccccgaa actgggtgcg
     1441 ctgctggtga acgccatagg cacggcagag gtgatcaccg cgctcgcccc gatctggact
     1501 gacaagccgg tcatggcccg caaggtgcga acccgcatcg gacaggtgct ggcattcgcc
     1561 aaggcgcgcg gctggcgcaa agatgcgctt cccgacgccc gagaactgcg ctcgggcctg
     1621 tccaagcaag gtcggggcgg aaacttcgca gcgatgcctt atgccgagtt gcctggcttc
     1681 gtggcgggtg aactagggcg ggaggctact gccagccgga cggcgatgct cttcgcagtt
     1741 ctgaccggcg ctcgctctgg cgaggttcgt aacgcgacgt gggagcaaat cgacttggat
     1801 agggggacct ggacccgtcc ggccgccatc atgaagatga aagaggggca cgttgttacc
     1861 ctgtcggcgg cagccattgc gttgttggaa cggtacactc ccaacgactt gcgtaagggc
     1921 ctggtcttct cgggcgttcg gggcggaccg ctgtctgata tgtcgctgac gaaggcgctg
     1981 cgcacggctg ggcgcgggga gacggtgcac ggattccgct ccgccttccg cgattgggcg
     2041 gccgagcaga tgccgactat tccggcgatg gtggctgaaa tggcgctcgc tcacaaagtt
     2101 gggaccaaga ccgaacaggc ctatctgcgc agcgaccttc gcgacatgcg ccgcgcccta
     2161 atggatgcat ggggccgctt cgctgcaccg tccttgtcgc ccggtggcag caacgtggtg
     2221 gacatgtccc gcacgggcca ggcttcacag cagcgagcag cgggcggtca atcatgagcg
     2281 atctcgaatt catcgtcttg ttcgttgcca tttacggcat tgggatagca gccgcatggt
     2341 ttgccgaccg atatgaggta cacgtgagtg cgatccggga agaagaatat ctgcggggct
     2401 tccatgatgg tcgcttcacg aagaagccac cgccccacat gtagggccgg ttgagcccgg
     2461 gacttagggc gcgagggagg cgcgagcccg ccttccaact ctgtccgccc gcgtagccag
     2521 tattctgccg tcctcaggca gctcctcgcc gagagtgatc cgcagtctgg acagcggact
     2581 ccccatgacc gagaacagcc cacgacggga acagaaaggc ggctcacgat gacagctgcg
     2641 accatgcgaa ctaaccttgg cgattgggaa aacccgccgg tcatccaaga ccaggtgatc
     2701 gcctacatgg agcagatggg cggcgcgatg atcctgagcg acatctaccg accactcggg
     2761 ctgccgcgct acacggtcaa ccgggtgctg ggccggctgc attccaaggg cgtcctgtcg
     2821 cgctacaaga taccggtccg gctacgccaa tatgatcgat gccaaggggg agccttgcca
     2881 gacggcgcca cccggcaatg ctacctctac agctttgcgg atgaggtcgt ggcgtgaggc
     2941 gcgtcgcggt cggcgcggcg ctcgcctggg cgggaatagt agggctg
//
LOCUS       DATNBV010000158         2141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_399755,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000158 DATNBV010000000
VERSION     DATNBV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399755"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..>2141
                     /locus_tag="VN627_03705"
     CDS             173..>2141
                     /locus_tag="VN627_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HXD02392.1"
                     /translation="MATKAVSGTGGASRRSAPSGKWRLVLRRSVRRALELGAALLLLG
                     AALFLALALASYRQTDPSASTAAGGVVLNWMGLPGAFVADRALFLFGPTAVLLVPLLV
                     AWGRRLWRFAEAEESGEELGEAASGGKHWWRSVMILLLAMALIGTVLSLAFTAPGGTL
                     PATMGGLAGLLGAKGLRALAAFLPEMARGWAILGAAIACLVGGAVLAARVFALDWASL
                     LALPRLFGRRRAEAASLGNLQGLEPAREAPMREPRAVAGEPAPETPRRPTEISDPTRP
                     IKPAQTLNPKRQGDLFDEYELPGLDLLADPPPNAQPKVDKLALERNARLLETVLDDFN
                     VKGEITAVRTGPVVTMYELEPAPGIKASRVIGLADDIARNMSAISARVASIPGRTVMG
                     IELPNADRQMVSFKELVGCDRFANSKGLLPIILGKDIAGEPIVADLATMPHLLVAGTT
                     GSGKSVGLNCILLSLLYRLTPAECRMILVDPKVLELKSYDNIPHLLSPVVTEPAKAVR
                     ALKWAVEEMERRYRMMSEISVRNLTGFNEKVRNAIAKGKPLGKRIPIGFDPDTGEELY
                     EEQQFEYQTLPQIVVIVDELADLMVTVGKEIEVLIQRLSQKSRAAGIHLIMATQRPSV
                     DVITGVIKANLPTRISFNVTSRIDSRTILGEQ"
BASE COUNT          323 a          725 c          767 g          326 t
ORIGIN      
        1 ggcccctccc gcctgcggga ggggagcaag acgaagcagg cttcgttgcg tggtgcgtgc
       61 ggcgttccgt tcaccgcaat aaaagcgccc ggattccctt ggattcttga cggggagtcg
      121 cccccgggcg cattatgcgc cgtgcacgga aggaaggggc gcaaagcagc cgatggcgac
      181 caaggcagta tccgggaccg gtggggcgag ccgccgttcc gcgccttcgg gcaagtggcg
      241 gctggtgctg cgccggtcgg tgcggcgggc gcttgagctg ggcgcggcgc tgctgctgct
      301 gggcgcggcg ctgttcctgg cgctggccct ggcctcctat cgccagaccg atccttcggc
      361 ctcgaccgcc gcgggcggcg tggtgctcaa ctggatgggc ctgcccggcg ccttcgtggc
      421 cgaccgcgcg ctgttcctgt tcgggccgac ggcggtgttg ctggtgccgc tgctggtcgc
      481 ctggggccgc cgcctgtggc gctttgccga ggcggaagag agcggcgagg aactgggcga
      541 ggcggcttcc ggcggcaagc actggtggcg ctcggtcatg atcctgctgc tggccatggc
      601 gctgatcggc acggtgctga gcctggcctt caccgcgccg ggcggcaccc tgccggcaac
      661 catgggcggc cttgccggcc tgctcggcgc caaaggcctg cgcgctctcg ccgcgttcct
      721 gcccgagatg gcgcggggct gggcgatcct gggcgccgcg attgcctgcc tggtgggggg
      781 cgcggtgctg gcggcccgcg tctttgcgct ggactgggcg agcctgctgg ccctgccccg
      841 gctgttcggg cgtcggcggg cggaggcggc gagccttggc aacctgcagg ggctcgagcc
      901 ggcccgcgaa gcgccgatgc gcgagccgcg cgcggttgcc ggtgaacccg cgcccgagac
      961 gccccggcgg ccgacggaga tcagcgatcc gacccgcccg atcaagccgg cgcaaacgct
     1021 caatcccaag cggcagggcg acctgttcga cgaatacgaa ctgcccggac tcgacctcct
     1081 ggccgatccg ccgcccaatg cgcagccgaa ggtcgacaag ctggcgctgg agcgcaacgc
     1141 ccgcctgctc gagaccgtgc tggatgactt caacgtcaag ggcgagatca cggccgtgcg
     1201 cacggggccg gtcgtgacca tgtacgagct tgagcctgcc cccggcatca aggccagccg
     1261 ggtaatcggc ctcgccgacg acatcgcccg caacatgagc gcgatctcgg cccgcgtcgc
     1321 ctcgatcccc ggccggacgg tcatgggcat cgagctgccc aatgccgacc ggcagatggt
     1381 cagcttcaag gagctggtcg gctgcgacag gttcgccaat tccaagggcc tgctgccgat
     1441 catcctgggc aaggacattg ccggcgagcc gatcgtggcg gacctggcga ccatgcctca
     1501 cctgctggtg gcgggtacga ccgggtcggg caagtcggtc ggcctcaact gcatcctgct
     1561 ctcgctgctc tatcggctga cgccggccga gtgccggatg atcctggtcg atcccaaggt
     1621 gctcgagctc aagtcctacg acaacatccc ccacctgctg agccccgtgg tgaccgagcc
     1681 ggccaaggcc gtgcgcgcat tgaagtgggc ggtcgaggag atggagcggc gctaccggat
     1741 gatgagcgag atctcggtgc gcaaccttac ggggttcaac gagaaggtcc gcaacgcgat
     1801 cgccaagggc aagccgctgg gcaagcgcat cccgatcggg ttcgacccgg atacgggcga
     1861 ggagctctac gaggagcagc agttcgaata ccagaccctg ccgcagatcg tggtgatcgt
     1921 cgacgagctg gcggacctga tggtcaccgt cggcaaggag atcgaggtgc tgatccagcg
     1981 cctcagccag aagagccgcg ccgccggcat ccacctgatc atggccaccc agcgcccgtc
     2041 tgtcgacgtc atcaccgggg tgatcaaggc caacctgccg acacggatca gcttcaacgt
     2101 caccagccgc atcgacagcc gcacgatcct cggcgagcag g
//
LOCUS       DATNBV010000159         2412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1066272,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000159 DATNBV010000000
VERSION     DATNBV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066272"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /gene="uraD"
                     /locus_tag="VN627_03710"
     CDS             complement(<1..311)
                     /gene="uraD"
                     /locus_tag="VN627_03710"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HXD02393.1"
                     /translation="MAQTFKQPPGSLDRAAFVALYGRVYEHSPWIAEAAWSAGLTAEH
                     DTVEGLHQTLAAIVDAAPRERQLALLCAHPDLAGRLAVRGELTVESTSEQASAGLDKC
                     TP"
     gene            complement(311..1510)
                     /locus_tag="VN627_03715"
     CDS             complement(311..1510)
                     /locus_tag="VN627_03715"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HXD02394.1"
                     /translation="MDPIVADWISLALRWAHVMVGIMWIGTSFHFIWLDASLRREPGM
                     GEGLAGASWMVHGGGFYRATKFLVAPPEMPADLHWFKYEAYFTWITGFLLLAVIYYVG
                     ANDLLIDPRVMELTPGKAIGISLGGLAAGWILYDLLCRSPIGDRTGLLAFLVFLLACV
                     AAYGFTHVFSGRAAFVHIGAFLGTIMAANVFFIIIPNQKKVVAALIAGKIPDPALGKR
                     AKQRSLHNNYLTLPVVLMMISNHYPMMFEHAQSWLLVVGILLFSGLLRLYANMKNAGA
                     SGSPVTAILFYAAMVGIGAIAYASYRPDADLEAVGPVTFADVQAIMAHRCQACHSANP
                     TDAAYKVPPKGIAFDTADQIQRMAPQIERMVVLTHAMPLANKTGMLPEERLVIAKWLA
                     GGKTGVD"
     gene            1652..>2412
                     /locus_tag="VN627_03720"
     CDS             1652..>2412
                     /locus_tag="VN627_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXD02395.1"
                     /translation="MPDTEDLAIFVRVVELGSLSAAGRDMRLSAAVVSNRIARLEKNV
                     GTRLLNRTTRHVSLTEDGSHYYEHCVAILNELEQAESALDAITAEPRGPIKVTVPSVF
                     GRLHIAPHIPQFIERHPQMQVRLHLTDMLVDLIQERIDLAIRIAELSDSTAIVRKLAN
                     NRRLIVASPDYLKRRGTPQVPEDLLDHNCLLLRFPGSKQYRWTLRGPDGPITLRVSGN
                     MDSNNGEALRDWCLAGHGLALKSLWEIVDDLNEGRL"
BASE COUNT          452 a          817 c          754 g          389 t
ORIGIN      
        1 ggcgtgcatt tatcgaggcc cgcgctcgcc tgctccgacg tcgattccac agtcagttcg
       61 ccgcgcaccg ccagccgccc cgccaggtcg ggatgggcgc agagcagcgc cagttgccgc
      121 tcccgcggtg ccgcatccac aattgcggcc agggtctggt gcagcccctc gaccgtgtcg
      181 tgctctgccg tcaatcccgc cgaccaggcg gcctcggcga tccacggcga atgctcatag
      241 acgcggccgt agagcgcgac gaaggcggcg cggtcgaggc tgcccggcgg ctgtttgaag
      301 gtctgcgcca tcaatccacg cccgtcttgc cgccggccag ccacttcgcg atcaccagcc
      361 gctcctccgg cagcatgccg gtcttgttgg cgagcggcat ggcgtgcgtc agcaccacca
      421 tgcgctcgat ctgcggcgcc atgcgctgga tctggtcggc ggtgtcgaag gcgatgccct
      481 tgggcggcac cttgtaggcc gcgtcggtcg ggttcgccga atggcaggcc tggcagcgat
      541 gcgccatgat cgcctgcacg tcggcgaagg tgaccggccc gaccgcttcc agatccgcgt
      601 ccggccgata gctggcatag gcgatcgcac cgatgccgac catcgccgcg tagaacagga
      661 tggccgttac cggcgacccg ctcgcgcctg cattcttcat gttggcatag agccgcaaga
      721 ggccgctgaa cagcaggatg ccgacgacga gcagccagct ctgcgcatgc tcgaacatca
      781 tcgggtaatg gttgctgatc atcatcagca ccacgggaag ggtgagataa ttgttgtgca
      841 gcgaacgctg cttggcgcgc ttgccgagcg cggggtccgg aatcttgccg gcgatcagcg
      901 cggccacgac cttcttctgg ttcgggatga tgatgaaaaa gacgttggca gccatgatgg
      961 tgccgaggaa cgcgccgata tgcacgaaag ccgcgcggcc gctgaacaca tgggtgaagc
     1021 cataggccgc cacgcaggcc agcagaaaca ccagaaaagc cagcagcccc gtgcggtccc
     1081 cgatcggcga gcgacagagc aggtcgtaga ggatccagcc cgccgccagc ccgccgaggc
     1141 tgatgccgat cgccttgccc ggcgtcaact ccatcacgcg cggatcgatc aacagatcat
     1201 tggcgccgac ataatagatg accgcgagca gcaggaagcc ggtgatccag gtgaaatagg
     1261 cttcgtattt gaaccagtga agatcggccg gcatttccgg cggcgccacc aggaacttgg
     1321 tcgcgcgata gaagccgccg ccatgcacca tccagcttgc gccggccagc ccctcgccca
     1381 tccccggctc gcgccgcagg ctggcatcca gccagatgaa atggaacgag gtgccgatcc
     1441 acatgatgcc gaccatgaca tgggcccagc gcaaggcgag gctgatccag tcggcaacaa
     1501 tcgggtccat ggcgatcccc ctaacggaat ccccgctcct acggcgcggg agaggggtga
     1561 ctgagccttc tcaatctatc aaacctggcc ctggcttccg acgccactac ctggcattct
     1621 actgaaaatc atcttcaaga aatcggcaac aatgcccgat accgaggatc tggccatttt
     1681 cgtgcgggtg gtggagctgg ggagcctgtc ggccgccggg cgggacatgc gcctgtcggc
     1741 ggccgtggtc agcaaccgga tcgcccggct ggaaaagaac gtgggcaccc gcctgctcaa
     1801 ccgcaccacc cggcatgtca gcctgaccga ggatggctcg cactattacg agcattgcgt
     1861 ggccatcctc aatgagctgg agcaggcgga aagcgcgctc gatgccatca cggcggagcc
     1921 gcgcggtccc atcaaggtga cggtcccatc cgtgttcgga cggctgcata tcgctcccca
     1981 tatcccgcag ttcatcgaac gccacccgca gatgcaggtg cgcctgcacc tcaccgacat
     2041 gctggtcgac ctgatccagg agcgcatcga cctcgccatc cgcatcgccg agctgtcgga
     2101 ttcgaccgcc atcgtgcgca agctggccaa taaccgccgc ctgatcgtgg cctcgcccga
     2161 ctacctgaag cgtcgcggca cgccgcaggt gccggaggat ctgctggacc acaactgcct
     2221 gctgctgcgc tttccgggct cgaaacagta ccgctggacc ctgcgcggtc ccgacggacc
     2281 gatcacgctc cgcgtctccg gcaacatgga cagcaacaac ggcgaagccc tgcgcgactg
     2341 gtgtctcgcc ggccacggcc tcgcgctcaa gtcattgtgg gagatcgtcg acgacctgaa
     2401 cgaaggccgg ct
//
LOCUS       DATNBV010000160        10850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1066330,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000160 DATNBV010000000
VERSION     DATNBV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10850
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066330"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VN627_03725"
     CDS             <1..1110
                     /locus_tag="VN627_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXD02396.1"
                     /translation="RQAEDESELRETLAEQDVAPTDVVVIRRSIAADPPSRKRLARRS
                     MRHDDPLPDDVLIAAIRVQGSPQWLVARVFVPPAERLLLTTLLGQTLFIYAVLVSAMW
                     LLLRRITRPLAALTARLERFAETRDPDGQLLAEGPDDIARLIAAHNAMEDRIALLLDE
                     KDVMLGAIGHDLKTPLAALRVRIESVDDEGERARMAATIEDITRTLDDILSLARVGRP
                     SDPVERTELAALVLSVVEEYEDMGQPVDLGETTRVALPLRPTWIRRALRNLIGNALRY
                     GARATVSVTREGDAAVIRIADEGPGIPDDQIARMLEPFTRGDPSRNSETGGAGLGLTL
                     ARAIAEQHGGTLTLANRTDSAGRVTGLEAVLRLPV"
     gene            complement(1107..2681)
                     /locus_tag="VN627_03730"
     CDS             complement(1107..2681)
                     /locus_tag="VN627_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HXD02397.1"
                     /translation="MDDIFLGLASNGERQVLRLGRANRHGLVAGATGTGKTVTLQTVA
                     EQFSGAGVPVFLADVKGDLSGICMPGSHEFKHADSLEQRARDLGITDYAYKDNPAVFW
                     DLYGEKGHPIRTTVSEMGPLLLSRLLGLNETQEGVLTIAFRYADDNSLLLLDFDDLQQ
                     VLIACAEQASDLTTKYGNISKASVGTIQRQLLTFASQGADRFFGEPAFEINDFLKTDD
                     QGRGYINILSADKLMQSPKLYATFLLWLLSELFEALPEVGDPEKPGLVFFFDEAHLLF
                     DNAPAALFDKVEQVVRLIRSKGVGVYFISQNPIDIPEKVAGQLGNRVQHALRAFTPRD
                     QKAIKAAAETFRINPALDVETAITELKVGEALVSTLDMEGAPSVVQRTLIAPPRSRLG
                     PVTDKERAIIQSISPFAGKYDTAVNRESAAEVLAQKAQDAAAAAQVVQEQGVDAQRAQ
                     PRQTPSMWEKAGKAAFGAAAASAGTMLARSLTGRKSGGSPVASAASAAAGSIGTALGG
                     ALLGRFARGLLGGLLR"
     gene            2789..4609
                     /gene="pabB"
                     /locus_tag="VN627_03735"
     CDS             2789..4609
                     /gene="pabB"
                     /locus_tag="VN627_03735"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HXD02398.1"
                     /translation="MAAPLPSSSFVLLDDARGPEARPARLFRAPREVVVARHADEVGP
                     ALERIEELRQAGLHLAGMLAYEAGLALEARLAGSTAARAGAAGPLVWFGAFAECEEIA
                     ASEVPGWLGRQASGPAGIGPLDPAISAGTYARACGRVHEAIAAGDIYQANLTFPLVGV
                     WRGDPLAIYAALRESGGGGHGGIVFDGSHWLLSLSPEQFFALEGEAATVSPMKGTRPR
                     PSDPEADSAADAAAAAELAGSAKDRAENLMIVDLMRNDLSRVALPGTVRVDAPFRVES
                     YPTVHQLVSTVTARLAPGRGAGDLIGALFPCGSITGAPKIRAMELIGELERDARGAYC
                     GAIGAIAPTGEATFNVAIRTLRLTPDPGGAARGRAVLGVGSGLVADSQCLPEWRECLV
                     KGGFVRSSAADFDLIETMAFTPDEGIALLELHLERAKASAAQLGFSFDRHAIRNAIQA
                     LCFEAEEPAKVRLTMARSGAYALEIGALPAALPGPARCAVLPLPVDAGDLRLRHKTSD
                     RQFYAAALRAAKAAGGDEALFIDDAGRLTEGSFTNLFVERGGTLLTPPAARGLLPGVL
                     RRSLIEAGRASEADLTLDDLAGGFLLGNALRGLLPATLLS"
     gene            4623..5801
                     /locus_tag="VN627_03740"
     CDS             4623..5801
                     /locus_tag="VN627_03740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXD02399.1"
                     /translation="MTASPHVSAALQRIAPSRTTAMTDRARELSEQGRDIISLSVGEP
                     DFATPDHVIAAAKAAMDAGDTKYTAVGGTARMKAAAALHFARDLGIAVPTSQVTISAG
                     GKQAIFHALLATLDPGDEVLIPAPWWVSYPEIVRFAGGTVVPLETNATGGFRITAAQL
                     SAAISPRTRWLLLNSPGNPTGATYSARELQALGEVLRAHPQVLVMSDDIYAPLRYSAG
                     AHATLAVECPDLTERVLTVSGVSKSHAMTGFRIGVAAGPRWLIAAMEKLQSHSSGNPC
                     SVSQAAAVAAFEGPQEFLADWRERFRARRDMVVAGINAIPGLSTPVPDGAFYCMIDAA
                     PLMGRFGDDDSLALHLLEHGVAIVAASGFGGRDGFRISFAADEAKLEEALRRIRGAVA
                     "
     gene            5798..6478
                     /locus_tag="VN627_03745"
     CDS             5798..6478
                     /locus_tag="VN627_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HXD02400.1"
                     /translation="MSELPILFEDAEALVIDKPAGLPLDQPRAGGMSLEDRLDDLRLG
                     FARPPAPVHRLDRDTSGCLLLARNPKALKRFAAAFEARLVEKRYLGIVGGPVSGEEGT
                     IALSLSKISSAADGWRMIVAKKGKPAVTHWRKLAENGGLTLVEFRPETGRTHQIRVHA
                     AHAFGHALLGDERYGGRPGPRTMLHAADLVVPRPGKEPVRAHAPLPADFVRLGFADPQ
                     DSLAKEPE"
     gene            6475..6900
                     /gene="arfB"
                     /locus_tag="VN627_03750"
     CDS             6475..6900
                     /gene="arfB"
                     /locus_tag="VN627_03750"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HXD02401.1"
                     /translation="MSAEAAAEEAIARAMAHASESFVTATGPGGQNVNKVATAVQLRV
                     NVYALRLAPPVFHRLKDLAGSRMTARGELLLVARQYRTQEQNRADARERLAQLLREAH
                     RLPEKRAKSRLNRVGKTQRLEGKKRRGTVKAGRGRIALD"
     gene            complement(6915..7109)
                     /locus_tag="VN627_03755"
     CDS             complement(6915..7109)
                     /locus_tag="VN627_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02402.1"
                     /translation="MRPAAAFVLLLTVGLAACAKPDNAPGPGGVTVGEARALDEAAEM
                     IESEQPPRIATAPKATQPAN"
     gene            complement(7166..7498)
                     /locus_tag="VN627_03760"
     CDS             complement(7166..7498)
                     /locus_tag="VN627_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02403.1"
                     /translation="MNKSISFALLGAASLLLAACGSSDTASEQATPDNVEMPAEETMS
                     TVAAMPSPDAGATEAADPNAGAKPNDAAAAAAAAAADFDAAGDGAEKPVTVKPGAEKA
                     IDQAEKKM"
     gene            7642..8643
                     /locus_tag="VN627_03765"
     CDS             7642..8643
                     /locus_tag="VN627_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXD02404.1"
                     /translation="MKLEPTLRALADLTRLRIMRLLADMELAVGELAQVLGQSQPRVS
                     RHIKILCDAGLAERRREGSWVFLRIAVDETGAPHLGTAVARLLATGEGEDPAFAARCA
                     EDRRHLAAIRAARESQAAGWFARHAAEWDTLRSLHSPDDLVEGALARQLGPRLGALLD
                     VGTGTGRMAELFAPAAERVAALDKSPEMLRLARARLQHLPAGKVDLLQGDFTALPFAE
                     AAFDTVLFHQVLHYAQEPEVALAEAARVTRPGGRVAVVDFAAHDREDLRTLHQHARLG
                     FSDEQMLALLTAAGFGPDQPLAIPGTGAGQPLTVKIWTATRPVATGGATDKTRKAVS"
     gene            8640..9530
                     /gene="metF"
                     /locus_tag="VN627_03770"
     CDS             8640..9530
                     /gene="metF"
                     /locus_tag="VN627_03770"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HXD02405.1"
                     /translation="MIPTDLPGPAFADLPGDISVSFEFFPPKSEKMEAQLWEAITELA
                     PLDPSFVSVTYGAGGSTRERTHATVARIVNETALVPAAHLTCVGASKGEITEIADQYW
                     ESGVRHIVALRGDPSTGAGTKFEPHPQGYASAAELVAGLKARHDFEISVAAYPEVHPD
                     AASAEADLDNLKRKLDAGATRAITQFFFSTDAWFRFVDKALAAGISAPILPGIMPVTS
                     FAAIRRMSANTEIPAAMETMFEGLDERPRPRALVSAVAAADLCQRLYAGGVRDFHFYT
                     LNKAEQAYAICHLLGLRPKQ"
     gene            9527..10591
                     /locus_tag="VN627_03775"
     CDS             9527..10591
                     /locus_tag="VN627_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HXD02406.1"
                     /translation="MTQLTPSAARIAFQAEAAKRILITDGAQGTEFQKMGLTEADYAG
                     NLGLSHDQKGNNDMLCLASPQAVEKVHRDYFDAGADIAETNTFSSNRISQADYGAEHL
                     VRELNVAAAGIARRIADEYAAKDGRPRFVAGGIGPTNKTLSLSPDVNDPGYREIDWDF
                     LVDVYKEQAAALMEGGADFILIETIFDTLNAKAGIMACKQLERELGREVPIMLSMTLT
                     DLSGRNLSGHTVEAFWHAVRHARPLTVGLNCSFGATQLRPHVKLLSEIADSLIMVYPN
                     AGLPNELGQYDEQPVTTAGLVHEWAAAGQVNVLGGCCGSTPAHIAEMARAVQGLAPRT
                     VPVLDHVTRLAGLEPFTLAA"
BASE COUNT         1643 a         3793 c         3763 g         1651 t
ORIGIN      
        1 cgccaggccg aggacgagag cgaactgcgc gaaaccctgg ccgagcagga cgtggcgccg
       61 accgatgtcg tggtcatccg ccgcagcatc gcggcagatc cgccatcgcg caaacggctg
      121 gcccgccgga gcatgcggca cgacgaccct ctgcccgatg acgtcctgat cgccgcaatc
      181 cgcgtgcaag gctcaccgca gtggctggtc gcccgcgtct tcgtgccgcc cgcggagcgc
      241 ctgctgttga cgacgctgct ggggcagacc ctgttcatct acgccgtgct ggtgagcgcg
      301 atgtggctgc tgctgcgccg gatcacccgc cccctcgccg cgctgaccgc ccggctcgag
      361 cggttcgccg agacccgcga ccctgatggc cagctcctag ccgaagggcc ggacgacatt
      421 gcccgcctga ttgccgcgca caacgccatg gaagaccgca tcgccctgct gctcgacgaa
      481 aaggacgtga tgctcggcgc catcgggcat gacctcaaga ccccgctggc cgccctcagg
      541 gtgcgcatcg aatcggttga tgacgagggc gagcgggccc gcatggccgc aacgatcgag
      601 gacatcaccc gcaccctcga cgatatcctc tccctcgccc gcgttggccg gccgagcgat
      661 ccggtggagc gcaccgagct cgccgccctc gtgctctccg tggtcgagga atacgaggac
      721 atgggccagc cggtcgacct gggcgaaacc acccgcgtgg cgctgccgct gcgccccacc
      781 tggatccgcc gggccctgcg caacctcatc ggcaatgccc tgcgctatgg cgcgcgggcc
      841 acggtctcgg tcacccgcga aggggatgcg gcggtcatcc ggatcgccga cgagggaccc
      901 ggaattccgg acgaccagat cgcccgcatg ctcgaaccct tcacccgcgg agacccttca
      961 cgcaacagcg agacaggcgg cgcagggctt ggccttacgc ttgcgcgggc gattgccgaa
     1021 cagcacggcg gcacgcttac ccttgccaac cggaccgaca gcgccggccg cgtgaccggc
     1081 ctcgaagccg tgctccggct gcccgtttag cgcagcagcc cgccgagcag gccgcgggcg
     1141 aagcggccga gcagggctcc gccaagggcc gtgccgatcg aacccgccgc cgcgctggcc
     1201 gcgctggcca cgggcgagcc gccggacttc cggccggtga gcgagcgggc gagcatggtc
     1261 cccgccgaag ccgccgccgc gccgaaggcc gccttgccgg ccttttccca catgctcggc
     1321 gtctgccgcg gctgggcacg ctgggcgtcg acgccttgtt cctgcaccac ttgggcggcc
     1381 gccgcagcgt cctgcgcctt ttgcgcgaga acctcggcgg cggattcccg gttcaccgcc
     1441 gtgtcgtact tgccggcaaa ggggctgatt gactgaatga tcgcccgctc cttgtcggtt
     1501 accggcccca gccgcgagcg gggcggcgcg atcagggtgc gctggaccac cgacggcgcg
     1561 ccttccatgt cgagcgtgga aaccagcgcc tcgcccacct tgagttcggt gatcgccgtt
     1621 tcgacgtcga gcgcggggtt gatccggaag gtctcggccg ccgccttgat cgccttctgg
     1681 tcgcgcgggg tgaaggcgcg cagggcgtgc tgcacccggt tgcccagctg accggcgact
     1741 ttctccggga tatcaatggg attctgcgag atgaaataga cacccacgcc cttggagcgg
     1801 atcagccgga ccacctgctc gaccttgtcg aacagcgccg ccggggcgtt gtcgaacagg
     1861 agatgggcct cgtcgaagaa gaacacgagc ccgggctttt ccgggtcgcc gacttcgggc
     1921 aaggcctcga acaattcgga caggagccag agcaggaagg tcgcgtaaag cttggggctt
     1981 tgcatcagct tgtcggcgga gaggatgttg atgtagcccc ggccctgatc gtcggtcttg
     2041 aggaagtcgt tgatttcaaa ggccggttcc ccgaagaacc ggtctgctcc ctggctggcg
     2101 aaggtcagca gctggcgctg gatcgtgccg accgaagcct ttgagatgtt gccgtatttt
     2161 gtggtgagat cgctcgcctg ctcggcgcag gcgatcagga cctgctggag atcgtcaaaa
     2221 tccagcaaaa gcaggctgtt atcatcggca taacggaagg cgatggtgag cacgccttcc
     2281 tgggtctcgt tgagcccgag gaggcgggag aggaggagcg ggcccatttc cgacacggtg
     2341 gtgcggatcg ggtggccctt ttcgccgtag agatcccaga acaccgccgg gttgtccttg
     2401 taggcatagt cggtgatgcc gaggtccctg gcccgctgct cgagcgaatc ggcgtgcttg
     2461 aattcgtggc tcccgggcat gcaaatgcct gaaagatcgc ccttaacatc ggccaggaag
     2521 accggcaccc cggcaccgga gaactgttcg gcgaccgtct gcagggtcac cgtcttgccg
     2581 gttccggtgg cgccggcgac caggccgtgg cggttggccc ggccgagacg aagcacctgc
     2641 cgctccccat ttgaggccag cccgaggaag atatcgtcca ttgccgctcc ttggcgcaag
     2701 agtagcgacc ctcccgcaag ggcaaggaat agccgcctct tcgcgccttt tgcaacgccg
     2761 ctgcaacacc actacaagcc gccgcgccat ggctgcaccg ctcccgtcct cttccttcgt
     2821 cctgctcgac gatgcccgcg gcccggaagc ccggccggca cgcctgtttc gtgcccctcg
     2881 ggaggtggtc gtcgcccgcc atgcggacga ggtcggcccc gcgctggagc ggatcgagga
     2941 gctgcgccag gcgggcctgc acctggctgg catgctcgcc tacgaggcgg gcctggcgct
     3001 ggaggcgcgg cttgccggga gcaccgccgc ccgcgcaggg gcggcagggc ccctggtgtg
     3061 gttcggggcc ttcgccgaat gcgaggagat cgccgcgagc gaggtgccgg gctggctggg
     3121 gcggcaggcc agcggcccag cggggatcgg cccgctcgac ccggcgatat ccgccgggac
     3181 ctatgcccgc gcttgcgggc gggtccacga ggcgattgcg gcgggggaca tctaccaggc
     3241 caacctcacc ttcccgctgg tcggcgtctg gcgcggcgat ccgctggcga tctatgcggc
     3301 gctacgcgaa tcgggcgggg gcgggcacgg cgggatcgtg ttcgacggat cccactggct
     3361 gctcagcctc tcgcccgaac aattcttcgc actggagggc gaggcggcga cggtcagccc
     3421 gatgaagggc acccggcccc gcccgtccga ccccgaggcc gacagcgccg ccgatgccgc
     3481 agcggcggcc gagctggcgg ggagcgcgaa ggaccgggcg gaaaacctga tgatcgtcga
     3541 cctgatgcgc aacgacctgt cgcgcgtggc cctgcccggc acggtccggg tcgatgcgcc
     3601 gttccgggtc gaaagttatc cgaccgtcca ccagctcgtc tccacggtga ccgcgcggct
     3661 cgcgccgggg cgcggcgcgg gcgacctgat cggggcgctg tttccctgcg gttcgatcac
     3721 cggagcgccc aagatccggg cgatggaact gatcggcgaa ctggaacgcg atgcccgcgg
     3781 cgcctactgc ggcgcgattg gcgcgatcgc gcccactggc gaggcgacgt tcaatgtggc
     3841 aatccgcacg ctgcgcctta cccccgaccc gggcggagcg gcgcgaggtc gggcggtcct
     3901 tggcgtcggc tccggtctgg tcgccgattc gcaatgcctg ccggagtggc gggagtgtct
     3961 ggtcaaaggg ggtttcgtgc gcagttccgc cgccgatttc gacctgatcg agaccatggc
     4021 cttcacgccc gacgaaggca tcgcccttct ggaactccac ctcgaacggg ccaaggcgag
     4081 cgctgcgcag ctgggcttct cgttcgaccg ccacgccatc cgcaatgcca tccaggcgct
     4141 gtgctttgag gccgaggagc cggccaaggt tcgcctgaca atggcccgca gcggcgccta
     4201 tgcgctggaa atcggcgcac tgcctgcagc cctgcccggc ccggcccggt gtgcggtgct
     4261 gccgctgccg gtcgacgcgg gcgacctgcg cctgcgccac aagaccagcg accgccagtt
     4321 ctacgccgcc gccctgcgcg ccgccaaggc cgcgggcggg gacgaggccc tgttcatcga
     4381 cgatgcgggc cggctgaccg aggggtcctt taccaacctg ttcgtggagc gcggcggcac
     4441 gctgctgacc ccgcccgccg cgcgcggcct gctgccgggc gtgctgcgcc gctcgctgat
     4501 cgaggccggc cgcgccagcg aggcggacct gacgcttgac gatctcgccg gaggcttcct
     4561 cctcggcaat gccctgcgcg gcctcctgcc cgcgaccctg ctctcctgac tgaccggaac
     4621 cgatgaccgc ttctccccat gtctccgccg cgctccagcg catcgcgccc tcacgcacca
     4681 cggcgatgac cgaccgcgcg cgggagctgt ccgaacaggg ccgcgacatc atctcgctct
     4741 cggtgggcga gcccgacttc gccacgccgg accatgtgat tgccgcagcc aaggcggcga
     4801 tggacgcggg cgacacgaag tataccgccg tgggcggcac ggcccggatg aaggcggcgg
     4861 ccgcgctcca ctttgcccgc gacctcggga tcgcggtgcc gaccagccag gtgacgatca
     4921 gtgccggggg caagcaggcg atcttccatg ccctgctcgc cacgctcgac ccgggcgacg
     4981 aggtgctgat ccccgcgccg tggtgggttt cctaccccga gatcgtccgc tttgccgggg
     5041 gcacggtcgt cccgctggaa accaatgcca caggcggctt ccgcatcacc gccgcccagc
     5101 tgtctgccgc gatcagcccg cgcacccgct ggctgctgct caacagcccc ggcaacccca
     5161 cgggcgcaac ctattcggca cgggaactgc aggcattggg cgaagtgctg cgcgcccatc
     5221 cccaggtgct ggtgatgagc gacgacatct atgctccgct gcgctactcc gccggcgccc
     5281 atgccacgct ggcggtggaa tgcccggacc tgaccgagcg ggtgctgacc gtctccggcg
     5341 tgtccaagag ccatgccatg accggcttcc gcatcggcgt cgccgccggg ccgcgctggc
     5401 tgatcgcggc gatggagaag ctgcagtcgc actcgtcggg caatccctgt tcggtctcgc
     5461 aggccgccgc cgtagccgcc ttcgaaggcc cgcaggaatt cctggctgac tggcgcgagc
     5521 gcttccgcgc ccggcgcgac atggtggtgg ccgggatcaa tgccattccc gggctgtcta
     5581 ccccggtgcc ggacggagcc ttttactgca tgatcgatgc cgcgccgctt atgggccgct
     5641 tcggcgacga cgactccctg gcgctgcacc tgctcgagca cggggtggca atcgttgccg
     5701 catccggctt tggcggcagg gacggctttc gcatctcctt cgccgccgat gaggcgaagc
     5761 tggaagaggc cctgcggcgg atcaggggag ccgtggcatg agcgagctgc ccatcctgtt
     5821 cgaagacgcc gaggcgctgg tgatcgacaa gcccgccggc ctgccgctgg accagccccg
     5881 cgccgggggc atgagcctgg aggaccggct cgacgacttg cggctggggt ttgcccggcc
     5941 accggcgccg gtccaccggc tggaccgcga cacttccggc tgcctgttgc tggcgcgcaa
     6001 tcccaaggcc ctgaagcgct ttgcggccgc cttcgaggcg cggctggtgg agaagcgcta
     6061 tctcgggatc gtgggcggcc cggttagcgg agaagaaggc acgattgcgc tctcgctgtc
     6121 caagatcagc agcgccgccg atggctggcg gatgatcgtg gcgaagaagg gcaagccggc
     6181 ggtgacccac tggcgcaagc tggccgagaa tggcgggctg accctggtcg aattccgccc
     6241 ggaaaccggc cgcactcacc agatccgcgt ccatgccgcc catgccttcg gccatgccct
     6301 gcttggcgac gagcgctacg gcggcaggcc ggggccgcga accatgctcc atgcggcaga
     6361 cctggtggtg ccgcgccctg gcaaggagcc ggtgcgcgcc catgctccgc tgccggcgga
     6421 cttcgtgcgg ctgggctttg ccgatccgca ggacagcctg gcgaaggagc cggaatgagc
     6481 gccgaggcag ccgccgaaga ggcgattgcc cgggccatgg cccatgccag cgaaagcttc
     6541 gtcacggcca cgggcccggg cgggcagaac gtcaacaagg tggcgacggc agtgcagctg
     6601 cgggtcaacg tctatgcctt gcggctggcc ccgccggtgt tccatcgcct gaaggacctc
     6661 gccggatcgc ggatgaccgc gcggggcgag ctgctgctgg tggcccggca ataccgcacc
     6721 caggagcaga accgcgccga tgcgcgcgaa cgcttggcgc agctgctgcg cgaagcgcac
     6781 cggctgccgg aaaagcgggc caagagccgg ctcaaccggg tgggcaagac ccagcggctg
     6841 gagggcaaga agcgccgcgg gacggtcaag gccgggcggg ggcggattgc gcttgattga
     6901 gcgggcgggc gtcgtcagtt cgctggctgg gttgccttcg gcgccgtggc gatgcggggt
     6961 ggctgttcgc tttcgatcat ctcggccgct tcgtcgagcg cgcgggcctc gcccacggtg
     7021 acgccgcccg ggcccggcgc attatcgggc ttggcgcagg cggcaaggcc tacggtcagc
     7081 aggaggacaa aggcggcggc cgggcgcatc ggggcgtgtg gcaggtgcgg gcggcaggag
     7141 cgggcgccgg gcccgcccct gtggctcaca tcttcttttc ggcctggtcg atcgcctttt
     7201 cggcgcccgg cttgaccgta accggctttt cggcgccatc gccggcggcg tcgaaatcgg
     7261 ccgccgcggc ggcggcggca gcggcggcgt cattgggctt ggcgcccgcg ttgggatcgg
     7321 ccgcttcggt ggcgccggca tcgggcgagg gcatggcggc gaccgtgctc atggtttctt
     7381 cggccggcat ctcgacattg tcgggcgtgg cctgttcgct tgcggtgtcg gagctgccgc
     7441 aggcggcgag gaggagcgag gcggcgccga gcagcgcaaa ggagatggac ttgttcatgg
     7501 gcggcggcct ttcggatggc agggcaagtt gcgcagtcct ctagccgagg caggggcccg
     7561 ggcaaagccc cgaggctgcg gatttgcgac gtggtgcgca cccttgcaga gatataaaga
     7621 aatctttata tgcgcctcgc aatgaagctc gaacccaccc tgcgcgccct ggcggacctg
     7681 acccgactga ggatcatgcg cctgctggcc gacatggagc tggcggtggg cgaactggcg
     7741 caggtgctgg ggcagagcca gccgcgagtc tcgcgccaca tcaagatcct gtgcgatgcg
     7801 ggcctggccg agcggcggcg cgaaggctcg tgggtgttcc tgcggatcgc ggtggacgaa
     7861 accggggctc cgcacctcgg cacggccgtg gcccggctcc tcgccacagg cgagggcgag
     7921 gaccccgcct tcgccgcccg ctgcgcggag gatcgccggc acctggccgc gattcgcgcc
     7981 gcgcgcgaat cgcaggccgc gggctggttc gcccgccacg cggccgagtg ggacacgctg
     8041 cgttcgctcc attcgcccga cgatctggtc gaaggcgcgc tggcccggca gttggggccc
     8101 cggcttggcg ccctgcttga cgtcggcacg gggacggggc gcatggccga gctgttcgca
     8161 cccgccgccg agcgcgtcgc cgcgctcgac aagagcccgg aaatgctgcg ccttgcccgc
     8221 gcgcggctgc agcacctgcc ggcgggcaag gtcgacctgc tgcagggcga tttcaccgca
     8281 ttgccctttg ccgaggccgc gttcgatacg gtgctgtttc atcaggtgct gcactatgcg
     8341 caggagcccg aggtggccct ggccgaggcg gcgcgggtca cccgcccggg cggacgcgtg
     8401 gccgtggtcg atttcgccgc gcacgaccgc gaggatctgc gcaccctgca ccagcacgcc
     8461 cggctcggct tttccgacga gcagatgctg gccctgctga ccgccgccgg ctttggcccg
     8521 gaccagccgc tggcaatccc cggcaccggg gccggccagc cgctcacggt caagatctgg
     8581 actgccaccc gccccgtcgc gaccggcggt gcgaccgaca agacccgaaa ggctgtttcg
     8641 tgattcccac tgacctcccc ggccccgcgt ttgccgacct gccgggcgat atctcggttt
     8701 ccttcgaatt cttcccgccc aagagcgaga agatggaagc ccagctgtgg gaggcgatca
     8761 ccgagctggc gccgctggac ccgagctttg tctccgtcac ctatggcgcg ggcggatcga
     8821 cccgcgagcg gacccatgcg accgtggcgc ggatcgtcaa cgaaaccgcg ctggttcccg
     8881 cggcgcacct gacctgcgtg ggcgcgagca agggcgagat caccgagatc gccgaccagt
     8941 actgggaatc cggcgtgcgc cacatcgtcg cgctgcgcgg ggatccctcg accggagccg
     9001 gcacgaagtt cgagccgcat ccgcagggct atgccagcgc ggcggaactg gtggcagggc
     9061 tgaaggcgcg gcacgacttc gagatttcgg tggcggccta tccggaagtc catcccgatg
     9121 ccgccagcgc ggaggccgac ctcgacaatc tcaagcgcaa gctcgacgcc ggggcgaccc
     9181 gcgcgatcac ccagttcttc ttctcgaccg atgcctggtt ccgctttgtc gacaaggctc
     9241 tcgcagcagg catttccgcg ccgatcctcc cggggatcat gccggtcacc tcgttcgccg
     9301 cgatccgccg gatgagcgcc aacaccgaga taccggcggc gatggaaacc atgttcgagg
     9361 gcctcgacga acgcccccgc ccgcgcgcgc tcgtctccgc cgtagccgcc gccgacctgt
     9421 gccagcggct ctacgccggc ggggtgcgcg acttccattt ctacacgctc aacaaggccg
     9481 agcaggccta tgcgatctgc cacctgctcg gattgaggcc gaaacaatga cccagcttac
     9541 ccccagcgcc gcccgaattg ccttccaggc cgaagccgcg aaacgcatcc tcatcaccga
     9601 cggcgcgcaa ggcaccgaat tccagaagat gggactcacc gaggcggact atgcgggcaa
     9661 cctcggcctg tcgcacgacc agaagggcaa caacgacatg ttgtgcttgg cgagcccgca
     9721 ggccgttgag aaggtccacc gcgactattt cgacgccggc gccgacattg ccgagaccaa
     9781 caccttctcc tccaaccgca tcagccaggc cgactacggc gccgagcatc tcgtgcgcga
     9841 gctcaacgtg gcggcggcag ggatcgcacg gcggatcgcg gacgaatacg ccgcgaagga
     9901 tggccggccg cgctttgtgg cgggcgggat cgggccgacc aacaagacgc tttccctttc
     9961 acccgacgtc aacgatccgg gctaccgcga gatcgattgg gatttcctgg tggacgtcta
    10021 caaggagcag gccgccgcgc tgatggaagg cggggcggac ttcatcctga tcgagacgat
    10081 tttcgacacg ctcaacgcca aggccgggat catggcctgc aagcagctcg agcgcgagct
    10141 tggccgcgag gtgccgatca tgctgtcgat gacgctgacc gacctttcgg gccgcaacct
    10201 ttcgggccac accgtcgagg ccttctggca cgcagtgcgc catgccaggc cgctgacagt
    10261 ggggctgaac tgctcgttcg gcgcgaccca gctgcggccg cacgtgaagc tgctgagcga
    10321 gatcgccgac agcctgatca tggtctatcc caatgcgggg ctgcccaatg agctcggcca
    10381 gtatgacgag cagccggtga ccaccgccgg gctggtccac gaatgggcgg cggccgggca
    10441 ggtcaacgtg ctcggcggat gctgcggatc gaccccggcg catatcgcgg agatggccag
    10501 ggccgtgcag ggcctcgccc cgcgcacggt gccggtgctg gaccatgtca cccggctggc
    10561 cgggctggaa ccattcacac tggcggcctg agcgaacccg ccccgccgca aggccggggc
    10621 accacaatct cccctcccgc ttgcgggagg ggctggggga gggcttgtta gggccacctt
    10681 ccgccagccg aaaccaccag gccctccccc gacccctccc gcaagcggga ggggggagct
    10741 ttgatcatga cgcagacccc cacttccgcc cgcttcgtca acatcggcga acgcaccaac
    10801 gtcaccggct cggccgcctt caagaagctg atcctcgccg gcgactacgc
//
LOCUS       DATNBV010000161         4629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2957828,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000161 DATNBV010000000
VERSION     DATNBV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2957828"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /gene="nth"
                     /locus_tag="VN627_03780"
     CDS             complement(<1..628)
                     /gene="nth"
                     /locus_tag="VN627_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXD02407.1"
                     /translation="MKPAEIAEIFDRFAAADPDPRGELAFINPYTLLVAVVLSAQATD
                     ASVNKATEPLFKLVDTPAAMVKLGETKLKEYIRTIGLYNTKAKNIIRLSQMLIEAHGG
                     AVPVDRDILETLPGVGRKTANVVLNVAFAHPTLAVDTHIFRVCNRTGLAPGKTPRAVE
                     DGLEKVVPPERKLHAHHWLILHGRYVCIARKPLCEKCIIADICKWPGKT"
     gene            727..1218
                     /locus_tag="VN627_03785"
     CDS             727..1218
                     /locus_tag="VN627_03785"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HXD02408.1"
                     /translation="MSDPAAEPVLFDALLTPHRSLSPRGFALLMAIAGLVGFGFGSAF
                     LVMGAWPIFGFCGAEWLLFYVCFRLNYRAARMRERVRLTPALLTVERRDPRGKTQSWS
                     FQPYWLRVEMDDPPEHGSQLSLASHGKRLTIGSFLSPPERQDLAEALREALARARQAP
                     AAG"
     gene            1277..2185
                     /locus_tag="VN627_03790"
     CDS             1277..2185
                     /locus_tag="VN627_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXD02409.1"
                     /translation="MAASGRDYVLLLLLSAMWGGSFLLIKLAVATLPPLWVASLRIAV
                     GGLALLLVLRLRGLALPRGRAVWARLAFMGIVGNLAPFALIGWGELQVASGLAAILMA
                     MVPLMVMVIAHFRMPDEPLTPGKIAGIVLGIIGVVVLIGPSALSGVGSHVAAELAILA
                     ATACYAASALAARGLPPMRADAMSAGMLLVAAPVGLIAAGLIDSPASLAPSLESLLAA
                     ALLGLLCTGLGYVLFFRILTSAGAGFASMNNFLVPPFGVIYGWMVLSEHLPASAFAAL
                     ALILLGLVALRWRLSPSPPERGRGPG"
     gene            complement(2190..2996)
                     /gene="dapB"
                     /locus_tag="VN627_03795"
     CDS             complement(2190..2996)
                     /gene="dapB"
                     /locus_tag="VN627_03795"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HXD02410.1"
                     /translation="MTDCRIGVVGAAGRMGRVLLRLAATTPGYRLAAGSERPGAAELG
                     RDLGSLAGMEPLGLILGDNAEAVFAASDAVLDFTTPSAALAHAALAAASGKAHVIGTT
                     GIDAAGEAKLAEAAAGAPIVYAGNMSLGVNLLAELVTQVARSLDPAWDIEIIEMHHRH
                     KVDAPSGTALLLGRAAAAGRNVKLEEVAERGRDGITGARKSGTIGFTALRGGDVVGEH
                     SVIFATEGERIELTHKATSREIFARGALRAARWAAGRKPGLYAMKDVLGL"
     gene            complement(3088..4227)
                     /gene="dnaJ"
                     /locus_tag="VN627_03800"
     CDS             complement(3088..4227)
                     /gene="dnaJ"
                     /locus_tag="VN627_03800"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXD02411.1"
                     /translation="MAKQDYYDTLGVGRDASAEQLKKAYRGLAMKFHPDRNPGDKNAE
                     HKFKEISEAYDVLKDDQKRAAYDRFGHRAFENGHGAGNAGGFDFAAGGGGFADIFEEM
                     FGEFMGGRRGQQARQRGGDLRYNMEITLEEAFAGKQATIRVGALGQCDVCAGSGAEKG
                     SKPITCPTCRGSGKVRHSQGFFTVETTCAGCQGGGKIIDKPCRSCGGQGRSRQDKTLN
                     VTIPPGVEDGTRIRLAGEGEAGLRGSPGGDLYIFLTVAPHKLFQREGANVYCRVPITM
                     TAAALGGIIEVPSVDGSRARITIPAGTQTGQQFRLRSKGMSVLRSHERGDMYAQIMVE
                     TPMHLTKRQQELLQEFENAGKGKQHSPEAEGFFAKVKEFWNELKE"
     gene            complement(4424..>4629)
                     /locus_tag="VN627_03805"
     CDS             complement(4424..>4629)
                     /locus_tag="VN627_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02412.1"
                     /translation="SQISMKIGEQLYKAQQPGGEGGPGNGPGGAHGGPAGGSSGNGQA
                     GGDEKVVDADFEEVDDRKGKQAN"
BASE COUNT          576 a         1651 c         1542 g          860 t
ORIGIN      
        1 ccgtcttgcc gggccatttg caaatgtcgg cgatgataca tttttcgcac aagggtttgc
       61 gagcgatgca gacgtagcgc ccgtgcagga tcagccagtg gtgggcgtgg agcttgcgtt
      121 ccggggggac gactttctcc agcccgtcct ccaccgcccg cggcgtcttt cccggcgcca
      181 ggccggtgcg gttgcagacc cggaagatat gggtgtcgac ggccagggtg gggtgggcga
      241 aggcgacgtt cagcaccaca ttggcggtct tgcggccgac ccccggcagg gtctcgagga
      301 tgtcgcggtc gaccggcacc gcgccgccat gcgcctcgat cagcatctgg ctgaggcgga
      361 tgatgttctt ggccttggtg ttgtagaggc cgatggtccg gatgtactcc ttgagcttcg
      421 tctcccccag cttcaccatc gccgcgggcg tatcgaccag cttgaacagc ggctcggtcg
      481 ccttgttgac gctggcgtcg gtcgcctggg ccgagagcac caccgccacc agcaaggtat
      541 aaggattgat gaaggcgagc tcgccccgcg gatcgggatc agcggccgcg aagcggtcga
      601 agatctcggc gatttcggcc ggcttcagcc gcccgctgga ttttcggacc ttcttcgcca
      661 tggcctgctc ccccatccgc ttgcctgagt ctagccctcc ggcggcgcgg actctatatt
      721 ccagccatga gcgatcccgc cgcagagccg gtgctgttcg acgccctgct cacccctcat
      781 cgcagcctga gtccccgggg cttcgccttg ctgatggcca tcgccgggct ggtgggcttc
      841 ggcttcggct cagcctttct ggtcatgggc gcctggccca tcttcggctt ctgcggtgcc
      901 gaatggctgc tgttctatgt ctgcttccgc ctcaattacc gggcggcgcg gatgcgggag
      961 cgcgtgcggc tcacaccggc cctgctcacc gtcgagcgcc gcgatccccg cggcaagacc
     1021 cagtcctgga gcttccagcc ctattggctc cgggtcgaga tggatgatcc gcccgagcat
     1081 gggagccagc tttccctggc ctcccatggc aagcgcctga ccatcggcag ctttctctcg
     1141 ccgcccgagc gtcaggatct ggccgaggcc ctgcgcgagg ccctggcccg cgccaggcag
     1201 gcccccgccg ccggctgagc ggcgcccagc cgcccagccc tggccccaca caagcatggc
     1261 ggatcggcct atccctttgg cggccagcgg ccgcgactat gtcctgctgc tgctcctctc
     1321 ggccatgtgg ggcgggtcct ttctgctgat caagcttgcc gtcgccactt tgccgccgct
     1381 ctgggtcgcc tctctcagga tcgcggtcgg cgggctggcg ctgctcctgg tgctgcggct
     1441 gcggggcctg gcgctgccgc gggggcgggc cgtctgggcc cgcctggcct tcatggggat
     1501 cgtgggcaat ctcgccccct tcgccctgat cggctggggc gagcttcagg tcgccagcgg
     1561 cctcgcggcc attctcatgg ccatggtgcc gctgatggtc atggtgattg cgcatttccg
     1621 catgccggac gagccgctca cccccggcaa gatcgccggc atcgtcctcg ggatcattgg
     1681 cgtcgtcgtg ctcatcggcc cttcggcgct ctcgggggtg ggcagccatg tggcggcgga
     1741 gctggcgatc ctcgccgcca ccgcctgcta tgccgcctcg gcgctcgccg cccgcggcct
     1801 gccgccgatg cgggccgatg cgatgagcgc cggcatgctt ctcgtcgcgg cacccgtcgg
     1861 cctcatcgcc gccggcctga tcgattcgcc ggcgagcctc gcgcccagcc tggaatcatt
     1921 gctggctgcc gctttgctgg gactgctctg caccggcctc ggctatgtgc tgttcttccg
     1981 catcctgacc agcgccgggg cgggcttcgc ctccatgaac aacttcctgg tgccgccttt
     2041 cggcgtcatc tatggctgga tggtgctgtc ggagcatctg ccggcctcgg ccttcgccgc
     2101 tctggcgctg atcctgctgg gcctggtggc gctgcggtgg cgcctttccc cctcgccccc
     2161 ggaacggggg agaggaccgg ggtgagcgct cacagcccca gcacgtcctt catggcatag
     2221 agtccgggct tgcggccggc ggcccagcgg gcggcgcgca gcgcgccgcg ggcgaagatc
     2281 tcgcggctgg tggccttgtg ggtgagctcg atccgctcgc cctcggtggc gaagatcacg
     2341 ctatgctcgc cgacgacatc gccgccgcgc agcgcggtga agccgatggt cccgctcttc
     2401 cgcgcaccgg tgatgccgtc gcggccgcgc tcggccacct cctccagctt cacattgcgg
     2461 ccggcggcgg cggcccggcc gaggaggagg gccgtacccg agggcgcgtc gaccttgtgg
     2521 cggtgatgca tctcgatgat ctcgatgtcc caggcgggat cgagggatcg ggcgacctgg
     2581 gtcaccagct ccgccagaag attgaccccc aggctcatat tcccggcata gacgatgggc
     2641 gccccggccg cggcctcggc cagcttcgcc tcgcccgccg catcgatccc ggtggtgccg
     2701 atgacatggg ccttgcccga ggctgcggcg agggcggcat gggccagggc cgccgagggg
     2761 gtcgtgaaat ccagcaccgc atcggatgcg gcgaagaccg cctcggcatt atcgcccagt
     2821 atcaggccca ggggctccat tccggcgagg ctgcccagat cgcgcccgag ctcggccgcc
     2881 ccgggccgct cgctgcccgc cgccaggcga tagcccgggg tggtggcggc gaggcgcagc
     2941 agcacccggc ccattcgccc ggccgcgccg acgacgccga tacggcaatc ggtcatgatt
     3001 gtgatccccc gcttctatcc cgcttggcgg gacggacccc ctcaccccgg ccctctcccc
     3061 ctttcagggg gcgagggggt gagggagcta ctccttcagc tcgttccaga attccttgac
     3121 cttggcgaag aagccttcgg cctcgggact gtgctgcttg cctttgccgg cattctcgaa
     3181 ttcctgcagc agctcctgct ggcgcttggt cagatgcatg ggcgtttcga ccatgatctg
     3241 ggcatacatg tcgccgcgct catgggagcg cagcacgctc atgcctttgg agcgcaggcg
     3301 aaattgctgg ccggtctggg tcccggcggg aatggtgatg cgcgcccggc tgccgtccac
     3361 cgagggcacc tcgatgatgc cgcccagcgc cgccgcggtc atggtgatgg ggacgcggca
     3421 gtaaacattg gcgccctcgc gctgaaacag cttatggggc gccacggtga ggaagatgta
     3481 gagatcgccc ccgggcgagc cgcgcaggcc ggcctcgccc tcgcccgcca ggcggatgcg
     3541 ggtcccgtcc tccacccccg gcgggatggt gacgttgagg gtcttgtcct ggcggctgcg
     3601 gccctggccg ccgcaggagc ggcagggctt gtcgatgatc ttgccgccgc cctggcagcc
     3661 ggcgcaggtc gtctcgacgg tgaagaagcc ctgggaatgg cggaccttgc cgctgccgcg
     3721 gcaggtcggg caggtgatgg gcttggagcc cttctccgcc ccgctgccgg cgcagacgtc
     3781 gcattggccc agcgcgccga cgcggatcgt cgcctgcttg cccgcgaagg cctcctccag
     3841 ggtgatttcc atgttgtagc gcagatcgcc gccgcgctgg cgcgcctgct ggccgcgccg
     3901 cccgcccatg aactcgccga acatctcctc gaagatatcg gcgaagccgc cgcccccggc
     3961 ggcgaaatcg aagccgcccg cattgcccgc gccatggccg ttctcgaagg cccggtggcc
     4021 gaagcggtca taggccgcac gcttctgatc gtccttcagg acgtcatacg cctcgctgat
     4081 ttccttgaat ttgtgctcgg cgttcttgtc ccccggattg cggtccggat ggaacttcat
     4141 cgccaggccc cgataggcct tcttcagctg ctcggccgag gcgtcgcgcc caacaccgag
     4201 ggtatcgtaa tagtcctgct tcgccatggc ttgtcacgca cccgccggcc ggctcatcca
     4261 ccagcgccgc agcatccccc tttttccgcg atctgcaaga ccacccccgc ggtcggtcct
     4321 gccgatcctc tgtcgctttg aacctgaggg gacagcgcct tccctctccc gcaggatcgg
     4381 gaaggcgccg tcggcctcga aggttttcgg cgggccagca ggtttagttg gcctgcttgc
     4441 ccttgcggtc gtcgacctct tcgaagtcgg cgtccaccac tttctcgtcg ccgccggcct
     4501 ggccattgcc ggagctgccg cccgcggggc ccccatgggc gcctcctggg ccatttccgg
     4561 ggccgccttc gccgcccggc tgctgcgcct tatagagctg ctcaccgatc ttcatggaga
     4621 tctgcgaca
//
LOCUS       DATNBV010000162         7059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2655415,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000162 DATNBV010000000
VERSION     DATNBV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2655415"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VN627_03810"
     CDS             complement(<1..221)
                     /locus_tag="VN627_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HXD02413.1"
                     /translation="MTDLTELGVAAIREGVAKGDFTAVEVAEAFNANVAAAQGSLNAF
                     IVATPEAALEAARKVDADRAGGKALGKMAG"
     gene            complement(223..510)
                     /gene="gatC"
                     /locus_tag="VN627_03815"
     CDS             complement(223..510)
                     /gene="gatC"
                     /locus_tag="VN627_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HXD02414.1"
                     /translation="MSVDTATVAKIASLARLKVTEADLAAMVPELNGILAWVEQLGEV
                     DVTGVEPMTAVIPNHLRLREDAVTDGGIRDAVLANAPAPEHGFFGVPKVIE"
     gene            complement(535..1074)
                     /locus_tag="VN627_03820"
     CDS             complement(535..1074)
                     /locus_tag="VN627_03820"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXD02415.1"
                     /translation="MTEITRVPLQPLEKGSLAKLWLGVAAAVAVAAGAAWAAMPAMVS
                     VKTVQAGEGANPTLDDVAFIDYTGKLANGTVFDQGKAPLALREVVPGFTQALEQMQAG
                     GKYKVMIPAKLAYGDKATGPIPANSDLYFDIEVLGVMSRQQFEQQRQMMQMMQGMQGH
                     GGAGAGAGAEGAPEAAPKP"
     gene            complement(1188..1394)
                     /gene="rpsU"
                     /locus_tag="VN627_03825"
     CDS             complement(1188..1394)
                     /gene="rpsU"
                     /locus_tag="VN627_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXD02416.1"
                     /translation="MQILVRDNNVDQALRALKKKLQREGVYREMKLRRHYEKPSEKRA
                     REKAAAVRRARKLERKRAERDGAK"
     gene            1747..2160
                     /locus_tag="VN627_03830"
     CDS             1747..2160
                     /locus_tag="VN627_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HXD02417.1"
                     /translation="MSSPPTFASSSLLVAAGGGLGAWLRYCTGRVWLSAIGPVAASAF
                     PWATLTANVLGSLAMGLLVGWLARYGSAGGEAWRLLLGVGVLGGYTTFSSFALEFALF
                     VERGALASAALYVGLSLAAGFAALFAGLGLARVMI"
     gene            2157..3410
                     /locus_tag="VN627_03835"
     CDS             2157..3410
                     /locus_tag="VN627_03835"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXD02418.1"
                     /translation="MSGNDDQVRQFTVGPDDDGARLDRWFKRNLPQIGFATVSRWART
                     GQVRVDGKRADPADRVVKGQVIRVPPGGSEPAGKAPRARKPLTEDQLATADAMVIHRD
                     RSAIVLNKPPGLATQGGSGTFEHVDGLLDAYVRGDGPRPRLVHRLDKDTSGVLLIAAT
                     PGSAAFFSRRFSGRSARKIYWALVVGIPDIKDGLIELPLSKQPGTGGEKMMVDESGEG
                     QPARTRYRVLDRAGNSAAWVELQPLTGRTHQLRVHMAAIGHPIVGDGKYGGQAAFLTG
                     SISRKMHLHARRLIIEHPDGAPLDVTAELPEHFANSMAQLGFDEADGAELADAPKVDE
                     KEARKTAAKAHSKQYRKERRGERRKRSEAPAANRKPTGQRAGKPAGKAPGKPVGNPAG
                     KSAGKPGTRPPARTGAPRKPGPRKD"
     gene            3410..4009
                     /locus_tag="VN627_03840"
     CDS             3410..4009
                     /locus_tag="VN627_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HXD02419.1"
                     /translation="MHPNPAFRHEDRALLEALVDEIGFGMVFAATPDGPRVAHTPLLS
                     TSDGAVQFHLSRGNGLARWLAGETALVVVNGPDAYVSPRFSGNPAQVPTWNYVAVELE
                     GRIRRMDSDGLAGLLEALAARHEQRLSGEPWTMAEMPETELRRMMSGIVGFELEVEAW
                     RPTFKLSQNKPPAERKAIATALQAAGSPALAELMLRLVP"
     gene            4006..4662
                     /locus_tag="VN627_03845"
     CDS             4006..4662
                     /locus_tag="VN627_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXD02420.1"
                     /translation="MTSPLAVFDCDGTLVDGQGAVVAAMESAFRQTGLAVPDPHLVRR
                     MVGLSLPQAVARLAREASAEQVREAVAAYKTAFRAAREAGTLAEPLFPGIAALLDDLA
                     AAGWTMAVATGKSDRGLAHCLASHGIADRFTSLQTAERHPSKPDPAMLEAALFETDTA
                     PEAAVMIGDTAYDMAMAAAIGVRAVGVAWGYHEGPELIAAGAAYVARDAADLKEYLLQ
                     "
     gene            4659..4895
                     /locus_tag="VN627_03850"
     CDS             4659..4895
                     /locus_tag="VN627_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02421.1"
                     /translation="MTAPASPERPVKDPARARFFAIALVRLSGAVMVLLGLLITERRL
                     DLPWALGVVLTVAGFFDVFVMPKILARRWRSPAA"
     gene            4892..5587
                     /locus_tag="VN627_03855"
     CDS             4892..5587
                     /locus_tag="VN627_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HXD02422.1"
                     /translation="MKRFYKEATIGERDGRWHVLLDGRAVKTVGGRAQSLPSAALAEA
                     MAAEWAAQGEEIDAATFFLRDLADYALDVVAARPEDAIAAIAPYGETDTLCYRADDGD
                     PLRREQDLVWEPLLAAAEARHALAFTRIGGIMHELQPEATRARIAQLLAALDPFQLAP
                     LQTLTTLAASLTLGLAALEPDADLAALWNAANLEEDWQAERWGRDDEAEARRERRREA
                     FHAAAKFATLARS"
     gene            5610..6014
                     /locus_tag="VN627_03860"
     CDS             5610..6014
                     /locus_tag="VN627_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD02423.1"
                     /translation="MSTPTTGELLVAMLRAIERNDMAAVAELTHPDVVFETPYGPGGA
                     MRTEGRKAVVALLTAVGETMFAEAKFTIDREYACADPAFAMAEYRSDVRLYNGGTYGN
                     RYIAVCEARDGQIILFREYFNPLPIMQSLTPA"
     gene            6092..>7059
                     /locus_tag="VN627_03865"
     CDS             6092..>7059
                     /locus_tag="VN627_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXD02424.1"
                     /translation="METTIRVDQGPLTFKHLILLVIALATGKPKDAPQGPPLRSNRPH
                     RDHSPASPDAAARGLHAATPTEIPARGWKEVLLRTWKEAGDDNVGLVSAGVAFYAFLA
                     LVPLLGAVVLTYGLFASPAGVARSMARLTTLLPQEVAQIIGQQLTQVVQTSGGKKGIG
                     LLVALALAIFGARSAVGGIITSLNIAYEEQEKRGFLFVNLLAIGMTAAMVVLIVLALG
                     AATGMALLDEALPHLPGVVTALLKLLSYAILAIIAAATAATLYRYGPSRDEAKWRWIT
                     PGTLLFALAWVVLTAGFGFYAANLGHYGATYGSLAGVVVLLTWTYLS"
BASE COUNT         1076 a         2583 c         2302 g         1098 t
ORIGIN      
        1 ccggccatct tgcccagcgc cttgccgccc gcgcggtcgg catcgacctt gcgcgcggcc
       61 tccagcgccg cttcgggcgt ggcgacgatg aaggcattca gcgatccttg cgccgcggcg
      121 acattggcgt tgaaggcttc cgccacttcg acggcggtaa agtcgccctt ggccacgccc
      181 tcgcggatcg cggcaacgcc cagttcggtc aggtcggtca tgttattcga tcaccttggg
      241 cacgccgaag aagccatgct ccggggccgg ggcattggcc agcaccgcat cgcggatgcc
      301 gccgtcggtc accgcatcct cgcgcaggcg caggtggttg gggatcaccg ccgtcatcgg
      361 ctcgaccccg gtcacatcga cctcgccgag ttgttcgacc caggcgagaa tgccgttgag
      421 ttcaggcacc atggcggcga ggtcggcctc ggtcaccttg aggcgggcga gcgaggcgat
      481 cttcgccacg gttgcggtat cgaccgacat gtcagtccct cgaatctgtg aaaatcaggg
      541 cttcggcgcg gcttccggag caccttcggc cccggcaccg gcaccggctc ccccatggcc
      601 ctgcatgccc tgcatcatct gcatcatctg gcgctgttgc tcgaactgct ggcggctcat
      661 cacgccgagc acctcgatat cgaagtaaag gtcggagttc gccgggatcg gcccggtcgc
      721 cttgtcgcca taggcgagct tggccgggat catgaccttg tacttgccac cggcctgcat
      781 ctgctcgagc gcctgggtga aaccggggac gacttcgcgc agggccagcg gggccttgcc
      841 ctggtcgaac accgtgccgt tggcgagctt gccggtatag tcgatgaagg ccacgtcatc
      901 gagcgtgggg ttcgcgccct cgccggcctg gaccgtcttg accgaaacca tcgccggcat
      961 ggcggcccag gccgccccgg cggcaaccgc caccgccgcg gcaacgccga gccagagctt
     1021 ggcgagcgag cccttttcga gcggctggag cggaacgcgg gtgatctcgg tcatcgattc
     1081 gtcctcatcg ggtcaggaac gcgcacagca aaaagggcgc ggaaaaatcc gcgccctctt
     1141 ggcggaaagc agcggccggt tcaataccgg ccagcgcgct tttcttctta cttcgcgccg
     1201 tcgcgctcag cgcgcttgcg ctcgagcttg cgggcgcggc gaacggcggc agccttttcg
     1261 cgggcgcgct tttccgaggg cttttcatag tgacggcgca gcttcatctc gcggtacacg
     1321 ccctcgcgct gcagcttctt cttgagcgcg cgcaaggcct ggtcgacgtt gttgtcgcgg
     1381 acgagaattt gcataaaccg aaacacctca caaagcagcg gacgagaacc ccgccggtca
     1441 gctggcgcgg gggagcatcc gatatgcaat accaaacgtg ccgaatctct cctgagtccg
     1501 gctcgaagaa gcgggccgct agcagaggcg gtcctcattg gcaagcgaga ggcggcgaat
     1561 gcctgcgccg ggccacccag ccacccggtc cgctcactac taccgtcatc ccgtaccgtc
     1621 atcctgaact tatttcagga tccatggcgc ggcgccggct tcaggatcac gccgcctttg
     1681 gacaccagac atcgcgctcc acccctgcac tggccaatcc gcccctcccc cgctaaaggc
     1741 accccgatga gttcaccccc caccttcgcc agctcctcgc tgctcgtcgc cgccggcggc
     1801 gggctgggcg cctggctgcg ctattgcacc ggccgcgtct ggctctccgc catcggcccg
     1861 gtcgccgcca gcgccttccc ctgggcgacg ctgaccgcca acgtcctcgg cagtctcgcc
     1921 atgggcctgc tggtcggctg gctcgcccgc tacggcagcg ccgggggcga ggcttggcgg
     1981 ctgctgctcg gcgtcggcgt gctcggcggc tacaccacct tttcctcctt cgcgctggag
     2041 ttcgccctgt tcgtggaacg cggcgcgctg gcttcggcgg ccttatatgt cggcctgtcg
     2101 ctggcggcgg gcttcgccgc cctgttcgcc ggcctcggcc ttgcccgggt gatgatatga
     2161 gcggcaatga cgatcaagtc cgccagttca ctgtcggccc cgatgacgac ggcgcccggc
     2221 tggaccgctg gttcaagcgc aacctgccgc agatcggctt tgccacggtc tcgcgctggg
     2281 cgcgcaccgg ccaggtgcgg gtggacggca aacgcgccga tccggcggac cgcgtggtca
     2341 agggccaggt catccgcgtg ccccccggcg gcagcgaacc cgccggcaag gcgccccgcg
     2401 cccgcaagcc gctgacggaa gaccagctgg ccacggccga tgccatggtc atccaccgcg
     2461 atcgttcggc catcgtgctc aacaagcccc cgggcctcgc cacgcagggc ggctcgggca
     2521 ccttcgagca tgtcgatggc ctgcttgacg cctatgtgcg cggggatggc ccgcgcccgc
     2581 gcctggtcca ccggctcgac aaggatacct ccggcgtcct cctcatcgcg gccactccgg
     2641 gcagcgcggc cttcttctcg cgccgcttct ccggccgctc ggcgcgcaag atctactggg
     2701 cgctggtcgt cggcatcccc gacatcaagg acggcctgat cgaactgccc ctctcgaagc
     2761 agccgggcac cggcggcgag aagatgatgg tcgacgaaag cggagagggc cagcccgccc
     2821 gcacccgcta tcgcgtgctt gaccgcgcgg gcaacagcgc cgcctgggtg gaactccagc
     2881 cgctgaccgg ccgcacccac cagctgcgcg tccacatggc ggcgatcggc caccccatcg
     2941 tgggcgacgg caaatacggc ggccaggcgg cattcctcac cggctcgatc agccgcaaga
     3001 tgcacctcca tgcccgccgc ctaatcatcg agcacccgga cggcgccccg ctggacgtca
     3061 ccgccgaact gcccgagcac ttcgccaact cgatggccca gctgggcttc gacgaggccg
     3121 acggcgccga gctggccgat gcgcccaagg tcgacgagaa ggaagccagg aaaaccgcgg
     3181 ccaaggccca ttccaagcag taccgcaagg agcgccgggg tgagcggcgc aagcgcagcg
     3241 aagcgcctgc ggccaaccgc aagcccaccg ggcaacgggc cggcaaaccc gccgggaagg
     3301 cacccggcaa gcccgtaggc aaccccgcag ggaagtccgc cggcaagccc ggcacccgcc
     3361 cgccggcccg caccggagcg ccgcgcaagc cgggtccccg gaaggactga tgcaccccaa
     3421 cccggccttc cgccacgagg accgcgccct gctcgaggcg ctggtcgacg agatcggctt
     3481 cggcatggtc ttcgcggcaa cgccggacgg gccgcgggtc gcccataccc cgctgctttc
     3541 caccagcgac ggggccgtgc agtttcacct gtcgcgcggg aatggcctgg cgcggtggct
     3601 ggcgggtgag acggcgctgg tcgtggtcaa cgggccggat gcctacgttt ccccccgctt
     3661 cagcggcaat ccggcgcagg tgccgacatg gaactacgtg gcggtggaac tggaagggcg
     3721 gatcaggcgc atggacagcg atgggctggc cggcctcctc gaagccctcg ccgcgcgtca
     3781 tgagcaacgg ttgagcggag aaccctggac catggccgaa atgccggaaa ccgaactgcg
     3841 gcggatgatg agcgggatcg tcggcttcga gctggaggtc gaggcctggc ggcccacatt
     3901 caagctgtcg cagaacaagc cgccagccga acgcaaggcc attgcaacgg cgctgcaagc
     3961 cgccggatcg cccgccctgg ccgaactgat gctgcggttg gtgccatgac cagcccgctg
     4021 gccgtgttcg actgcgatgg caccctcgtc gacggccagg gcgccgttgt cgccgccatg
     4081 gagagcgcct tccgccagac cggccttgcc gttccggatc cgcatcttgt ccgccgcatg
     4141 gtcggcctct ccctgcccca ggccgtggcg cggcttgccc gcgaggcgag cgcggagcag
     4201 gtgcgcgagg cagtcgccgc ttacaagacc gccttccgcg ccgcgcgcga ggcggggacc
     4261 ctggccgagc ccctgttccc cggcatcgcc gccctgctgg acgaccttgc cgccgccggc
     4321 tggaccatgg ctgttgccac cggcaagagc gaccggggcc tcgcccattg cctcgccagc
     4381 cacggcattg ccgatcgctt cacctcgctc cagacggccg aacgccatcc ctccaagccg
     4441 gacccggcca tgctcgaggc agccctgttc gagaccgata ccgcgcccga ggcagcggtg
     4501 atgatcggcg ataccgccta tgacatggca atggccgccg cgatcggcgt gcgcgcggtg
     4561 ggcgttgcct ggggctatca cgaagggccc gaactgatcg ccgccggcgc cgcctacgtg
     4621 gcccgcgacg cggccgacct caaggagtac ctgctgcaat gactgccccc gcctcgcccg
     4681 aaaggccggt caaggacccg gccagggccc ggttcttcgc catcgccctg gtgcgcctgt
     4741 caggcgcggt gatggtcctc ctggggctcc tgatcaccga gcgccggctc gacctgccct
     4801 gggcgctcgg cgtggtgctg accgtggccg ggttcttcga cgtattcgtc atgcccaaaa
     4861 tcctcgcccg gcgctggcgg agcccggctg cgtgaagcgg ttctacaagg aagcaaccat
     4921 tggcgagcgc gacgggcgct ggcatgtcct gctcgatggc cgggcggtga agaccgtcgg
     4981 cggccgagcg cagtccctgc ccagcgccgc gcttgccgaa gccatggccg ccgaatgggc
     5041 cgcgcagggc gaggaaatcg atgccgcgac cttcttcctg cgcgaccttg ccgactatgc
     5101 gctcgatgtc gtcgccgcgc ggcccgaaga cgccatcgcc gcgatcgcgc cctacggcga
     5161 gaccgatacc ctgtgctacc gggccgacga cggcgacccc ctgcgccgcg agcaggatct
     5221 ggtgtgggaa ccgctcctcg ccgcggcgga agcgcgccat gccctcgcct tcacgcggat
     5281 cggcgggatc atgcacgagc tgcagcccga agcgacgcgc gcccggatcg cccagctgct
     5341 cgccgccctc gatcccttcc agctggcgcc gctgcagacg ctcaccacgc tggccgcctc
     5401 gctcaccttg ggcctcgctg cgctggagcc ggatgccgat ctcgccgccc tgtggaatgc
     5461 cgccaacctc gaggaggact ggcaagccga acgctggggc cgcgatgacg aggccgaggc
     5521 ccggcgcgag cgccgccgcg aggcctttca tgcggccgcg aaatttgcca ccctcgcccg
     5581 cagttaaatc acaaaagcgg agagaagcga tgagcacccc caccaccggc gaactgctcg
     5641 tcgccatgct gcgcgcgatc gagcgcaacg atatggccgc cgttgccgaa ctgacccatc
     5701 ccgacgtcgt gttcgaaacg ccttatggtc cgggcggcgc aatgcggacc gaggggcgca
     5761 aagcggtcgt ggccctgctc acagccgtgg gcgaaaccat gttcgccgag gcaaagttca
     5821 cgatcgaccg ggaatatgcc tgcgccgatc ccgccttcgc catggccgaa taccgcagcg
     5881 acgtgcgtct ctacaacggc gggacctacg gcaatcgtta cattgccgtg tgcgaagccc
     5941 gcgacgggca gatcatcttg ttccgcgaat acttcaatcc gctgccgatc atgcagtcgc
     6001 tcacgccggc ttgagcctcc agggcctcgc cgggcacgtt cggcccggag cggatcggcc
     6061 tcccgcgcgt tgccccggct ggaggtgcgc gatggaaacc acgatccggg tcgaccaggg
     6121 cccgctcacg ttcaagcatc tgattctgct ggtaattgcc ctggccaccg gcaagcccaa
     6181 ggatgccccg cagggcccgc cattacgttc gaaccggccg caccgagacc actctcccgc
     6241 ctcgcccgat gccgccgccc gcgggctcca cgccgcgacg cccaccgaga tcccggccag
     6301 gggctggaag gaagtcctgc tgcgcacctg gaaggaagcg ggcgacgaca atgtcgggct
     6361 ggtctccgcc ggcgtcgcct tctacgcttt ccttgccctc gtccccctgc tcggcgccgt
     6421 agtgctgacc tatggcctgt tcgccagccc cgccggcgtc gcgaggtcga tggcccgctt
     6481 gaccacgctc ttgccgcagg aggtggcgca gatcatcggc cagcaactga cccaggtcgt
     6541 ccagacctcc ggcggcaaga agggcatcgg cttgctggtg gccctcgccc tggccatctt
     6601 cggcgcacgc agcgccgtgg gcgggatcat cacctcgctc aacatcgcct acgaggagca
     6661 ggaaaagcgc gggttcctgt tcgtcaacct gctggcaatc ggaatgacgg cggccatggt
     6721 cgtgctgatc gtcctcgcac tcggcgcggc cacgggcatg gccctgctcg acgaggccct
     6781 gccgcatctg cccggcgtgg tgacggccct gctcaagctg ttgtcctatg ccattctggc
     6841 gatcatcgcc gcggccacgg cggccacgct ctaccgctat ggcccctcgc gcgacgaggc
     6901 gaagtggcgc tggatcacgc cgggcacgct gctgttcgcc ctcgcctggg tggtgctgac
     6961 cgccggcttc ggcttctatg ccgccaacct tggccactat ggcgcgacct acggctcgct
     7021 cgccggggtc gtcgtcctgc tgacctggac ctatctgtc
//
LOCUS       DATNBV010000163         2522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_742720,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000163 DATNBV010000000
VERSION     DATNBV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742720"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..714)
                     /locus_tag="VN627_03870"
     CDS             complement(25..714)
                     /locus_tag="VN627_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXD02425.1"
                     /translation="MTKSRILVSSLCAVSLLSLSACVTDPNTGQQKVSRTAIGGGVGA
                     LGGLLLGGLIGGGTGRIIGAGIGGIAGAAYGHTLDKQIKELKEQTAGSGVDVTPVDNG
                     QAILVNLPEGVTFDVGSYSLKPEFRATLDKVAESLKQYPDSLIDVYGHTDSTGSDAYN
                     QTLSENRARTVANYLQMQGVNAARIRSQGYGETMPVADNATEEGRRKNRRVEIKIVPV
                     TQDDVARARGQ"
     gene            835..2142
                     /locus_tag="VN627_03875"
     CDS             835..2142
                     /locus_tag="VN627_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXD02426.1"
                     /translation="MTPFPWSDVLIIAGLILLNGLFSMSELAIVSARPARLKIAAERG
                     STGARCALALADNPGKFLSTVQIGITLIGIIAGAYSGASLGHPTAERLIAWGLPPKHA
                     EEIGFALVIAVTTYFSLVVGELVPKQVALRLAEPIAIYAALPMRILARVTAPLVWLLD
                     RSSTLLMRVIGLKSATEQEVTEEELHMMFAEATRSGVLEEEERAIMTGIMRLADRPVR
                     ELMTPRTVLDTIDRRATEADVRALLKETPHSLWPVTDGAPDRIVGVVKAKDVLSVLLA
                     GKRLQIARLMKKAEIVPDQLDAMDALRILQGADVAMAMVHDEYGHLEGVVTPADLLTA
                     IVGEFASYQDEDADPMVVTREDGSLLISGALPADALAARLELELPADRDYATAAGYVL
                     SVIKRLPGEGEHFHEQGWRFEVVDMDGRKIDKLLVSREDAEAG"
     gene            complement(2166..>2522)
                     /locus_tag="VN627_03880"
     CDS             complement(2166..>2522)
                     /locus_tag="VN627_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HXD02427.1"
                     /translation="RLAKPHAMAGMNVLCAETDEEAWYHASSMDQSFVALRTGTPGRL
                     KPPVAGYRESLPPHVLAGLDHTRQVSAIGSRDTVRAAIAEFVERTGVDELIVAGATFD
                     PAVRRRSLVLTMEAIA"
BASE COUNT          391 a          905 c          803 g          423 t
ORIGIN      
        1 ccgctggcgg cgaggatttg ccgcctactg cccccgcgcc cgggccacgt catcctgcgt
       61 caccggcacg atcttgatct ccacgcgccg gttcttgcgg cggccttcct cggtcgcatt
      121 gtcggccacc ggcatggtct cgccatagcc ctggctgcgg atgcgtgccg cgttcacgcc
      181 ctgcatctgc aggtagttcg ccacggtccg cgcgcggttc tccgaaaggg tctggttgta
      241 ggcgtccgag ccagttgaat cggtgtggcc gtaaacgtcg atcaggctgt cgggatactg
      301 cttgaggctc tcggccacct tgtccagcgt cgcccggaat tccggcttca ggctgtagga
      361 gccgacatcg aaggtcacgc cctccggcag gttgacgagg atcgcctggc cattgtcgac
      421 cggggtcacg tccacgcccg atccggcagt ctgctccttg agctccttga tctgcttgtc
      481 gagggtatgc ccataggccg ccccggcgat gccgccgatg cccgcgccga tgatccggcc
      541 cgtgccgccc ccgatcagcc caccgagcag gaggccgcca agcgcgccca cgccgccgcc
      601 gatggccgtg cgcgaaacct tctgctggcc ggtgttcgga tcggtcacgc aggccgaaag
      661 gctcagcagc gaaacggcgc agaggctgga gacgaggatg cgggatttgg tcatggcaga
      721 tgccctttct gctggtccgg gggcccggag atcgcgcgaa agtgaatcag ccaacctgat
      781 cccaaggtga acgaagggtt cgcgcctcgc gccatatctg ctagcgccgc ctgcgtgaca
      841 ccctttccct ggtccgatgt tctcataatc gcggggctga tcctgctgaa cggcctgttc
      901 tccatgtcgg aactggcgat cgtctcggcg cggcctgcac ggctcaagat cgcggcggag
      961 cgcgggtcga ccggggcgcg ctgcgccctc gccctggcgg acaatccggg caagttcctc
     1021 tccacggtcc agatcgggat cacgctcatc ggcatcatcg ccggtgccta ttccggggcc
     1081 agcctcggcc acccgacggc ggagcggctg attgcctggg gcctcccgcc caagcatgcc
     1141 gaggagatcg gcttcgccct ggtcatcgcg gtgaccacct acttcagcct cgttgtcggc
     1201 gagctcgtcc ccaagcaggt cgccttgcgc ctggccgaac cgatcgcgat ctacgccgcc
     1261 ctgccgatgc gcattctcgc ccgggtcacc gcgccgctgg tctggctgct cgaccgttcg
     1321 tccaccctgc tcatgcgcgt gatcggcctg aagtcggcca ccgagcagga ggtgaccgag
     1381 gaagagctgc acatgatgtt cgccgaggcc acccgctcgg gcgtgcttga agaggaagag
     1441 cgcgcgatca tgaccgggat catgcgcctc gccgatcgtc cggtgcgcga actgatgacc
     1501 ccgcgcaccg tgctcgacac gatcgaccgc cgggcgaccg aagccgatgt ccgcgccctg
     1561 ctcaaggaaa ccccgcattc gctctggccg gtaaccgacg gcgcgcccga ccggatcgtc
     1621 ggcgtggtca aggccaagga cgtgctctcg gttcttctcg ccggcaagcg cctgcagatc
     1681 gcccgcctga tgaagaaggc cgagatcgtg cccgaccagc tcgacgccat ggacgccctg
     1741 cgcatcctcc agggcgccga tgtcgccatg gccatggttc atgatgaata cggccacctc
     1801 gaaggcgtgg tcaccccggc ggacctgctc accgccatcg tcggcgaatt cgcctcctac
     1861 caggacgagg acgccgaccc gatggtggtc acgcgcgagg acggctcgct gctgatctcc
     1921 ggcgccctcc cggccgatgc ccttgccgcc cgcctcgaac tcgagctccc cgccgaccgc
     1981 gactatgcca cggccgcggg ctatgtcctc tcggtgatca agcgcctgcc gggcgagggc
     2041 gaacacttcc acgaacaggg ctggcgcttc gaggtggtcg acatggacgg ccgtaagatc
     2101 gacaagctgc tggtcagccg ggaggatgcg gaggcggggt gagcttgcag taaacaccca
     2161 actcctcagg caatagcctc catcgtcaac accagcgacc gccgccgcac cgcgggatcg
     2221 aacgtcgccc ccgccacgat cagctcgtcc accccggtcc gctcgacaaa ctccgcaatc
     2281 gccgcccgca ccgtgtcccg gctgccgatg gcggaaacct gccgggtgtg gtccagcccg
     2341 gccagcacgt gcggcggcag actctcacga tagcccgcca ccggcggctt caaccgcccc
     2401 ggcgtgccgg tgcgcagggc aacgaagctc tggtccatgg acgaggcatg gtaccatgcc
     2461 tcctcgtcgg tttcggcgca gagcacgttc atcccggcca tggcatgggg tttcgccagc
     2521 cg
//
LOCUS       DATNBV010000164         5680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2015792,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000164 DATNBV010000000
VERSION     DATNBV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015792"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..999
                     /gene="mmsB"
                     /locus_tag="VN627_03885"
     CDS             130..999
                     /gene="mmsB"
                     /locus_tag="VN627_03885"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HXD02428.1"
                     /translation="MKIAFIGLGNMGGGMAANLVKAGYEVRAFDLAPAACDRAAGSGC
                     TVVASIMDAVCDADAVVSMLPNGAIVEQVYGEDVIGQAPAHALLIDCSTIDIATTRSV
                     AGTASDAGYEMVDAPVSGGIAAANGGTLTFMVGGSSEAFARARPILSAMGKAVIHAGA
                     IGNGQAAKICNNMLLGATMIATCEAFRLAERLELDLQTFYDISSKASGQSWSMTSYCP
                     VPGVGPRSPADNGYQGGFAAGLMLKDLRLAMAAAAESDAHVPMAAMAEQLFARFVDEG
                     NAGMDFSGIIQTL"
     gene            complement(1063..1842)
                     /locus_tag="VN627_03890"
     CDS             complement(1063..1842)
                     /locus_tag="VN627_03890"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXD02429.1"
                     /translation="MGKLAGKVAIITGGARGMGAATSRLFVAEGAKIVIADVLEAEGA
                     ALAAELGEAARFYKLDVTSEDDWASVVAAAEAELGPVDVLVNNAGVVMFKALLDTTKA
                     DFEGVLQVNLVGQFLGLKAVAPGMIARGRGAIVNISSVDGMKGANCLAAYASSKWGVR
                     GLTKVAAMELGHKGIRVNSVHPGGVDTIMSNHTGAPRAELDKKYANVPLQRIGGPDEV
                     AAATAFLASDEASYIHGGEIVVDGGMTVGVYYDGFPGAPGM"
     gene            complement(1889..2344)
                     /locus_tag="VN627_03895"
     CDS             complement(1889..2344)
                     /locus_tag="VN627_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD02430.1"
                     /translation="MLSLQEISDRLEIQDLLARYSFAIDERDWDALDRVFTADAWVDY
                     TAAGGVKGSYPEIKAWLPEALARFPMFQHMVATTRLEIAGDTAGSRTILFNPMTYRAA
                     DGQDTVFFIGLWYRDRFARTADGWRIIERVEEMGWTHNAPAMPPVPDTQ"
     gene            complement(2423..3502)
                     /locus_tag="VN627_03900"
     CDS             complement(2423..3502)
                     /locus_tag="VN627_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXD02431.1"
                     /translation="MAKTKDYGLGEFDFPRGWFMIGAASDASRIPAAIRYFGKDLVLY
                     RGESGTPYVTDAYCPHMGAHLARNTTSYIVRDGEHVEGESIRCPFHGWRFGPDGVCNH
                     IPYSDFIPKAATLQTYPVIERAGILWMWHDPEGLEPDVELPDFGGHYGAPGWVEWKVD
                     YMGDLNSHGIEIVDNMADFGHFVPIHGATDWQYFANEFKDGHLHQYYSAGHRTLTANP
                     EDALVLDTWYEGPGFLQSEMAGTFDSFIMIANTPIDDGVTRAWHALMVKVHDGNRETT
                     AADRELALAYQEGSRLAFAQDVEIWANKRACINPLAIPKDGPYAKVRLWYRQFYNPRD
                     KAPEIQGRVNGLHVTLDKRADQKVA"
     gene            complement(3651..5336)
                     /locus_tag="VN627_03905"
     CDS             complement(3651..5336)
                     /locus_tag="VN627_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXD02432.1"
                     /translation="MNWNMEVDVVVVGSGAGGMVSALVAARNHAQVVVVEKDKLWGGT
                     SATSGGGIWIAGSDQAKAEGFEDNPEDAFTYLRALSADNVPDENIRAFVDNGAVMLRW
                     ITANTPVVYKAQPYPDYHAENPGGSVTGYRTHLPETFDGTPLGKDLKTQRFPSPAASL
                     FGYLNWTFAETYQLLYRSKGWLTGLIRNLAKYWFDWPLRFTSRKDRRLTLGNSLTGGL
                     RVALNEAGVPVWLRTPMKELVTEGGKVVGVVVERDGKPFRIAVRKGVVLGAGGFDKNQ
                     QMRDANAPLYPKAQLSGGVTSNTGDSIRAAAAIGAGTMNLQSAWAAPVFYVPGEDRGR
                     LCTIERALPGCIMVNQKGERYLNEAASYHIVGQQMARREREHGDGSPSWMVFDNRYRH
                     LYPMGPLLPLVPDWLQSGPVKSILKKGRTIAELARAIGADLATLEAQVERFNRHAAKG
                     EDPDFHRGEAAYDKMYGDYRNQPNPCLRPLTEGPFYAMPIYPGDIGTNGGIRTNARAQ
                     VVDESGQPIPGLYAVGNNAASAMGESYPGAGVTIGPAMTFGFIAARDMMGVNE"
     gene            complement(5478..>5680)
                     /locus_tag="VN627_03910"
     CDS             complement(5478..>5680)
                     /locus_tag="VN627_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXD02433.1"
                     /translation="PSRAELQAVQVVAFFNMLFGFFWSSPLIESLSGRPATDPVAMDL
                     QKSLLRSFIGYMEQASHAAAIS"
BASE COUNT          973 a         1807 c         1824 g         1076 t
ORIGIN      
        1 cgcatccacg agcaaatcaa tgagatcctg cgcttgatcg ttggcgcaga cctcctcaca
       61 tgaggtatcc cgcagtcagt tcgacccgct gtcgacccat ctgtcagctg atgagcaatg
      121 gagccgagca tgaaaatcgc gtttatcgga cttggcaaca tgggtggcgg catggcggca
      181 aacctggtca aggccgggta cgaagtgcgc gcgttcgacc ttgcccccgc tgcctgtgat
      241 cgggccgccg gaagcggctg taccgtcgtc gcatcgatca tggatgccgt gtgcgatgcc
      301 gacgcggtcg tgtcgatgct gcccaatggc gcgatcgtgg agcaggtcta cggcgaggac
      361 gtgatcgggc aggcccccgc ccatgccctg cttatcgact gctccacgat tgatattgca
      421 acgacgcgct cggttgccgg gacggcatcg gatgcggggt acgaaatggt cgatgcgccg
      481 gtgtccggcg gtatcgccgc ggctaacggc ggcacgctga ccttcatggt gggcggcagc
      541 agcgaagcct ttgcccgcgc tcgcccgatc ctgtccgcga tgggcaaggc ggtgatccat
      601 gccggcgcca tcggcaatgg gcaagcggcc aagatctgca acaacatgct gctgggcgcg
      661 accatgatcg ccacctgcga ggcatttcgc ctggccgagc ggttggaact cgacctccag
      721 accttctacg acatttcgtc aaaggcctcc ggccagagct ggtccatgac cagctattgc
      781 ccggttcccg gcgtgggtcc gcgaagcccg gccgacaacg gttaccaggg cgggttcgca
      841 gccggactga tgctcaagga tttgcgcctg gcaatggcgg cggcagccga gagtgacgcg
      901 catgtgccga tggcggcgat ggcagaacag ctgttcgcca ggttcgtgga cgaaggaaat
      961 gcgggaatgg acttttccgg catcatccag acgctgtaat cccggccgcc ggcgcgggcg
     1021 cgagggccga ccccctgcgc ttctcgcaaa agcgcgggcc ggctacatcc ccggcgcccc
     1081 cggaaagccg tcgtagtaga ctccgactgt catgccaccg tcgaccacga tctcaccgcc
     1141 gtggatatac gaggcttcgt cgctggccag aaaggcggta gcggcggcaa cttcgtctgg
     1201 tccaccaatt cgctgcagcg gcacattggc atacttcttg tcgagttccg ccctcggcgc
     1261 tccagtgtga ttggacatga tcgtgtccac accgcccggg tgcaccgaat tgacgcggat
     1321 acccttgtgt cccaattcca tcgcggcgac cttggtgagg ccgcgaacgc cccatttcga
     1381 cgaggcatag gcagccaggc agttggcccc cttcatgccg tcgaccgaag agatgttgac
     1441 gatagcccct cgtccccgcg cgatcatgcc cggagcaact gccttcaggc cgaggaactg
     1501 gccgacaagg ttgacttgca ggacgccttc aaagtcagcc ttcgtcgtgt cgagcagggc
     1561 cttgaacatt actacgccag cgttgttgac gagcacgtcg accgggccaa gttccgcttc
     1621 cgctgctgcg accacgctgg cccaatcatc ctcgctggtc acgtccagct tgtagaagcg
     1681 ggccgcttcg ccaagttccg cggcgagggc agcgccctcg gcttccagca cgtcggcgat
     1741 gacgatcttc gcgccttccg ccacgaacag ccgcgacgtt gcagcgccca tgccccgtgc
     1801 gccgcccgtg atgatggcga ccttgcctgc cagcttgccc atgttttctc ccggatactg
     1861 tctgatgttg cggtttgtcg gctgaagttc actgcgtgtc cggcacgggc ggcatcgccg
     1921 gggcgttatg ggtccagccc atctcctcga cccgttcgat gatgcgccag ccgtccgccg
     1981 tgcgcgcgaa gcgatcacgg taccacaagc cgatgaagaa gaccgtgtcc tgcccgtcag
     2041 ccgctcgata ggtcatcggg ttgaacagga tcgtccggct gcctgccgtg tcgccggcga
     2101 tctcgagacg ggttgtggcg accatgtgct ggaacatcgg aaagcgcgcc aaggcttccg
     2161 gcagccatgc cttgatctcc ggataggacc ccttcacgcc gcccgcggcc gtgtaatcaa
     2221 cccaggcatc ggcggtgaac acccggtcca gcgcgtccca gtcgcgttca tcgatcgcga
     2281 agctatagcg ggcgaggaga tcctggatct ccagccggtc cgatatttcc tgtaggctca
     2341 gcattgtgcg cgctctctgg ccgatcagcc gttgccggca tcgcgggggg agagtggtcc
     2401 ctccccgcga cgttaccctg cgtcaggcca ccttctggtc ggctcgcttg tcgagcgtca
     2461 catgcaggcc gttgacgcgg ccctggatct ccggcgcctt gtcgcgcggg ttgtagaact
     2521 ggcggtacca gaggcgcacc ttggcgtagg ggccgtcctt gggaatggcg agcgggttga
     2581 tgcaggcgcg cttgttggcc cagatctcga cgtcctgggc gaaggccagg cggctgccct
     2641 cctgataggc cagcgccagt tcgcggtctg ccgcggtggt ttcgcggttg ccgtcatgca
     2701 ccttgaccat gagcgcgtgc caggcccggg tgacgccgtc atcgatcggc gtgttcgcga
     2761 tcatgatgaa gctgtcgaag gttccggcca tctctgactg gaggaagccg gggccttcgt
     2821 accaggtgtc gagcaccagg gcatcctcgg ggttggcggt cagggtgcgg tggccggcgc
     2881 tgtaatactg atggaggtgc ccatccttga attcattggc gaaatattgc caatcggtgg
     2941 caccgtggat cggaacgaaa tggccgaaat ccgccatgtt gtcgacgatc tcgatgccat
     3001 ggctgttgag gtcgcccatg tagtcgacct tccactcgac ccagcccggg gcgccgtaat
     3061 ggccaccgaa atcgggcagt tcgacatcgg gttcgagccc ttccggatcg tgccacatcc
     3121 acaggatccc cgcgcgctcg attaccgggt aggtctggag cgtcgccgcc ttggggatga
     3181 agtccgaata gggaatgtgg ttgcagacgc catcgggacc gaagcgccag ccgtggaacg
     3241 ggcagcggat cgattcgcct tcgacgtgct caccatcgcg gacgatgtag gacgtggtgt
     3301 tgcgcgccag gtgggcgccc atgtgggggc agtaggcgtc ggtgacgtag ggcgtgccgc
     3361 tctcaccccg atagaggaca aggtccttgc cgaaatagcg gatcgcggcg gggatgcggg
     3421 aggcatcgct ggccgcgccg atcatgaacc agccgcgcgg gaaatcgaat tctcccaggc
     3481 catagtcctt ggttttcgcc atctgcatca ctccttttgc gctttcgagc cgaatccggc
     3541 gttctgtcgg cgcatattcc cgatatatac cgtctggatc ggcgatttgc gaattatttg
     3601 ccctactgcc gcatcgctct ggcgcattgc gcgaccgcaa aaacgctggt tcattcgttc
     3661 acgcccatca tgtcgcgtgc ggcgatgaag ccaaaggtca tggccgggcc gatcgtgacg
     3721 ccggcaccgg ggtagctttc acccatcgcc gacgccgcat tgttgcccac cgcgtagagg
     3781 ccggggatcg gctggccgct ctcgtccacg acctgggcgc gggcgttggt gcgaattccc
     3841 ccgttcgtgc cgatgtcgcc gggatagatc ggcatggcat agaatggccc ctcggtcagc
     3901 gggcgcaggc aggggttggg ttggttccgg taatcgccgt acatcttgtc ataggccgct
     3961 tcgccccggt ggaaatcggg gtcctcgccc ttggcggcat ggcggttgaa ccgctccacc
     4021 tgggcttcaa gcgtggccag gtcggccccg atggcgcgag ccagttcggc gatggtccgg
     4081 ccctttttca ggatcgattt gaccggaccg gactggagcc agtcaggcac gagggggagc
     4141 agcggcccca tcgggtagag atggcgatag cggttatcga acaccatcca gctcggggag
     4201 ccatcgccat gctcgcgctc gcgacgggcc atctgctggc cgacgatgtg gtatgacgcc
     4261 gcctcgttga ggtagcgctc gcccttctgg ttgaccatga tgcaacccgg cagggcccgt
     4321 tcgatggtgc agagccgccc ccggtcctca ccgggcacat agaacaccgg cgccgcccag
     4381 gccgattgca ggttcatcgt gcccgcaccg atcgccgcgg ccgcgcggat cgaatctccg
     4441 gtattgctgg tcacgccgcc cgacagctgc gccttgggat agagcggggc attggcgtcg
     4501 cgcatctgct ggttcttgtc gaacccgccc gcccccagga caacgccctt gcgcacggcg
     4561 atgcggaacg gcttgccatc gcgctcgacc accacgccga ccaccttgcc gccttcggta
     4621 acgagctcct tcattggcgt gcgcagccac accggaacgc ctgcctcgtt cagcgccacg
     4681 cgcaggccgc ccgtcaacga attgcccaag gtcaggcgcc ggtccttgcg cgaggtgaag
     4741 cgcagcggcc aatcgaacca gtactttgcg agattgcgga tcaggccggt caaccacccc
     4801 ttgctgcgat agagcaactg gtaggtttcc gcgaaagtcc agttgagata gccgaacagg
     4861 ctcgccgcgg gcgacgggaa gcgctgcgtt ttcagatcct tgccgagcgg ggtgccatca
     4921 aacgtctcgg gcaggtgcgt gcggtagccg gtgacggagc cgccggggtt ttccgcgtga
     4981 taatcgggat agggctgggc cttgtacaca accggcgtat tggcggtgat ccagcgcagc
     5041 atgaccgcgc cgttgtccac aaaggcgcgg atgttttcgt caggcacgtt atcggccgac
     5101 agggcgcgca gataggtgaa ggcatcttcg ggattgtcct cgaaaccttc ggccttggcc
     5161 tggtcgctgc cggcaatcca gatgcccccg cccgaagtgg cgctggtgcc gccccacagc
     5221 ttgtccttct ccaccacgac gacctgcgcg tggttcctgg ccgcgacgag ggcggagacc
     5281 atcccgccgg ctccagaccc gacgacgacg acatcgactt ccatgttcca gttcatcgtc
     5341 agcaatcctg caatcggccc ggtgggcggg ctctaatccc gtttacgaac gcatttttcg
     5401 gacaaaccgc accgatgtca actccatgcg taacatttgg taattgtcat tacgtgaatc
     5461 ggcgaaattg tagccgatca ggaaatggcc gccgcatgcg aggcctgttc catgtagccg
     5521 atgaacgagc ggagcaggga tttctggaga tccatcgcga ccgggtccgt agctggccgt
     5581 ccggagaggc tttctatcag gggggaggac cagaagaacc cgaacagcat gttgaagaac
     5641 gccaccacct ggacagcctg cagctccgcc cgcgagggca
//
LOCUS       DATNBV010000165        10892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4580516,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000165 DATNBV010000000
VERSION     DATNBV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4580516"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VN627_03915"
     CDS             <1..579
                     /locus_tag="VN627_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXD02434.1"
                     /translation="VGEDQKQHLELARDVAQKFNFDFCPEDAPVFTLPDPIIPPEAAR
                     IMSLRDGSAKMSKSDPSEMSRINLTDDADTIMTKVKKAKTDPEPLPSEAAGLEGRPEA
                     ANLVGIYSAMSGKSVAEILGEFGGKGFGAFKPALGELLVESLSPINARFVGLRSDQEM
                     LDAILARGASRARALAIPTLDKVYTALGLVRG"
     gene            969..1700
                     /locus_tag="VN627_03920"
     CDS             969..1700
                     /locus_tag="VN627_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02435.1"
                     /translation="MREGTYGPSPRERAISALLSLALCLALAFMIVRMGAMGPSLPAN
                     GSRLVAVDVSAHESDKPRQAQRSPAHHVAAASATAPVITPPPPVPATSPVPALNLIPL
                     TREQLASADIGRMPRRGPAAGPPAPQDGGADGGGSGDGPGGARLYAAEWVREPTNAEL
                     ATYIPHEAAAPGAWATIACRTIDRFHVEDCREMEESPPGSGLARGLRQAAWQFLVRPP
                     RVNGKALVGSWVRIRFEFSHAAGSG"
     gene            1840..2535
                     /locus_tag="VN627_03925"
     CDS             1840..2535
                     /locus_tag="VN627_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HXD02436.1"
                     /translation="MSRKKFEFTALGKSLKLAAVPLVLASLSACATGLDTSVSRFQSQ
                     MPAPAGQTFAVVADNPALAGGLEFAQYARLVEGQMVRLGYTPSDPAHATMIVRFDYGV
                     DRGRERIRSTGAGFRDPFWDPWYGYRGFGGGYYGRPYRWGAYGGWGFGWYDPWFDNDV
                     ESYTVYTSGVSLKIDRKVDGVRLFEGKAEAVSTSNRLQYLVPNLVEAMFTGFPGHSGE
                     TVRITVAPENAKH"
     gene            2603..3757
                     /locus_tag="VN627_03930"
     CDS             2603..3757
                     /locus_tag="VN627_03930"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXD02437.1"
                     /translation="MKAALFLRIALLIALPSALLAAPLGAQATSPRWSTVALADLHKW
                     VAAAPADALPAPDASALEAAERLGDGAAVDRAADGLALKLATMHLTGCCGANHAGWHI
                     VDTDSTADLPARIAAAVSGGTLDAFFAGLAPQNPDYAALRAAYAAEQDPGRKATLARN
                     MERWRWLPREPGSRYLLVNTAAFEVRYWSGGKLVDRRAVINGKVSSPTPIFAARVTGI
                     TFNPWWDIPPNIVREGIGKLARTNPAAARARGYVWSGGKFRQRPGPTNSLGLMKLVMP
                     NPFNIYLHDTPSKNLFAKPVRAFSHGCVRVSDALGFASVLLGEDRAAVDARVASGATA
                     TVSLPAAMPVYIAYFTAGLGPDGQVAFYPDIYGRDAAMGDMKDNKPFCAA"
     gene            complement(3780..4247)
                     /gene="dut"
                     /locus_tag="VN627_03935"
     CDS             complement(3780..4247)
                     /gene="dut"
                     /locus_tag="VN627_03935"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HXD02438.1"
                     /translation="MPSPNDPVPVLVRRLPHGAGLDLPAYATEGAAGMDVVSAEDVTI
                     APGARHAVATGLAVAIPPHFEIQVRPRSGLALKHGITVPNTPGTIDSDYRGELKVILI
                     NHGAEPFVIRRGDRVAQLVLAPVTRAGWLEVEHLEETARGEGGFGSTGGVAAL"
     gene            complement(4231..5886)
                     /locus_tag="VN627_03940"
     CDS             complement(4231..5886)
                     /locus_tag="VN627_03940"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0071513 - phosphopantothenoylcysteine
                     decarboxylase complex [Evidence IEA];
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate synthase"
                     /protein_id="HXD02439.1"
                     /translation="MAGPRILLIVGGGIAAYKSCELVRMIRKGGGSVTCVLTDGGAKF
                     VTPMALAALSENPVHTTLWDLRNEVEMGHIQLSREADLVAVCPATADLMAKMAAGIAD
                     DLATTLLLATDKPVLALPAMNVRMWQHAATQRNVASLRDAGVFVMDPDEGAMACGEFG
                     PGRLPEPDAVWRLIASWFTGPEALPMRDGAGWSAPEDEPFTPLDPLPTFANDEGDSGP
                     DVGLGGLSASLIGRAAGKGAKKAMALEALGEEEALSAPAAEPEAPAVPPPGAGALLAK
                     KGKGRAAPPTDPEALNHLVNERQSAPQPIPGDQFGISVGGQLPLEPAAPFVRPEDGPL
                     SGRHVLVTAGPTHEPIDPVRYIANRSSGQQGFAIAAAAARAGAQVTLVAGPVNLATPP
                     GVDRIDVESAREMQAAVKEALPADVAIMVAAVADWRTKDVATSKLKKRGSAPPVLMLT
                     ENPDILASVGSSPSLKPRLLIGFAAETDKVIEHARDKRKRKGADWIVANDVSFDSKGK
                     SVMGGKSNTVHIVTADGVENLPEMPKDMVATALVERIANALAE"
     gene            complement(5891..7036)
                     /locus_tag="VN627_03945"
     CDS             complement(5891..7036)
                     /locus_tag="VN627_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02440.1"
                     /translation="MAEPGVTTRYAAWPRRAALAALALWLALMAVAALTPIRAGDNDA
                     AAGARGPAAASATLQVRTRDEDLQLYDDVIARLRHGEGYYGVIAEEHRRHRFPLHPGF
                     AVRLPTLAVIEAALPARALAPLSVVLALGVLLAWWRKLGGDPAALPVRSLAMAALLIG
                     VTIGTTRYFFVLHELWAGQLLALSFGLHRPERGRWLGAWLAAALALAIREHALPFVLL
                     MAAFALWQRRWREGLAWAGLAVLFLAALAVHLHLVARMVVPSDPPSASWITLRGLSGW
                     LSMLVLSSNLRLLPHAVGAVLVVASLLGWSGWKGQAGLFGTLLQAGYGLAFMIAGRGD
                     NFYWGMVVAPTLLVGLAFLPATLRDLWRAANPGQIPIALGRQGVQRT"
     gene            complement(7029..8183)
                     /locus_tag="VN627_03950"
     CDS             complement(7029..8183)
                     /locus_tag="VN627_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02441.1"
                     /translation="MDTDDARLAGLEARPSFDRYAGFSQFMARVVLGLLAAFLLLAVI
                     APGYSPPPPEPPKITVTDASGHNVVKSDDNDLRLYRLIYQRLARGDNYYVAATEEQRA
                     NNYPVAPGLTVRLPTLAMVSVALGPVGLVALQVMLFVAMMLANARRLGLEAGGEDRRP
                     MALALLMAGIASGLSARYNMVHEIWAAQLIALSFGLHRPDEGRWGGAWIAAAAALAVR
                     ELVLPFVMLMAAWALWHRRWREGAAWIALIALFGAALMVHLHLAEAQIRPGDPVSPSW
                     FVLKGIAGLMYKVNNSTFLSLVPVPVAGIFVVLSLFGWTGWRTPMGNFGALLTLGYAF
                     AFSIAGRDNNFYWGILITPLLFMGAAFLPIALPSLWRAAGLARRREPALG"
     gene            complement(8150..9742)
                     /gene="ubiB"
                     /locus_tag="VN627_03955"
     CDS             complement(8150..9742)
                     /gene="ubiB"
                     /locus_tag="VN627_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HXD02442.1"
                     /translation="MTTATTHILRLLKWGRILARHGALRIIEEGFNTPAPVRRLCKLA
                     RFGTRQPRIPDYAGAFQAIGPAAIKLGQTLATRPDLVGEEAAHNLLTLQDSLPPVAFD
                     EIRTEIEASFQQPLEALFASVEREPVGAASIAQVHRAVTADGRTVAVKVLRPGIARKF
                     ARDIDTYEWAAAHLEALGGEAERLRPRLVVENLKRWTARELDLRREAASASELAEAMK
                     SFAGYHVPEIDWDRTNGKVLTIEWVDGAKMSDLAALRAAGHDLPELAGRLVLAFLTQA
                     ISVGFFHADMHQGNLFVRPDGTIVAIDFGIMGRIDRRARQWLAEILYGLTTGNYRRVA
                     EIHFEAQYVPSYHSVDEFATALRAVGEPMRGRPVSELSVGQMLDGLFAITRDFDMAVQ
                     PHLLLLQKTMVMVEGLAQALDPAINMWDVSAPFVRSWIRDELGPEAAIAEKLREDFQT
                     LLRLPDLVRRVEERFPAKGGAPEAPPLRDIGLVWGGRSSGAEAVRQPDGNHMRASWPG
                     YLLAGIGGGLVTWILMTHGWPG"
     gene            complement(9746..10492)
                     /locus_tag="VN627_03960"
     CDS             complement(9746..10492)
                     /locus_tag="VN627_03960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXD02443.1"
                     /translation="MNDQVSSDHVSFGYEEVSPEEKTRRVGAVFSSVARKYDVMNDAM
                     SAGLHRLWKDKFVRRVKPRPGESILDMAGGTGDIAFRMARHGALVTVSDINEDMLAVG
                     MDRAAKKAIQGLTWSPQNAETLTFPDRAFDAYTIAFGIRNVTRIDAALAEAHRVLKFG
                     GRFFCLEFSTTEWPGFKEAYDAYSHHLVPKLGQMIAGDAESYRYLIESIRRFPPMPRF
                     AQMIRDAGFSRVKVEPILGGLVAIHSGWKA"
     gene            complement(10489..>10892)
                     /locus_tag="VN627_03965"
     CDS             complement(10489..>10892)
                     /locus_tag="VN627_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXD02444.1"
                     /translation="PITVIALTFAVAALALAPFAAWEWRSGQVVHWGPGVFGAFAYVA
                     LLASLSAYFLFNAAAARTGPVVAGQAITLLPLFGAVLSALLLGEVLHLYHLWGMALIV
                     VGIALGAFAAARGRPESPSGAMPAARLEDAP"
BASE COUNT         1733 a         3563 c         3999 g         1597 t
ORIGIN      
        1 gtgggcgagg accagaagca gcacctcgag ctggcgcgcg acgtggcgca gaagttcaac
       61 ttcgacttct gcccggagga cgcgccggtg ttcaccctgc ccgacccgat catcccgccc
      121 gaagccgcgc ggatcatgtc gctgcgcgac ggcagcgcca agatgagcaa gtccgatccc
      181 tcggaaatga gccggatcaa cctgaccgac gatgccgaca cgatcatgac caaggtcaag
      241 aaggccaaga ccgatcccga gccgctgccc tccgaggccg ccgggctgga ggggcggccg
      301 gaagcggcga acctggttgg catctattcg gccatgtcgg gcaagagcgt ggccgagatc
      361 ctcggcgaat tcggcggcaa gggcttcggc gcgttcaagc cggcgctggg cgaactgctg
      421 gtggaaagcc ttagccccat caacgcacgg ttcgtgggcc tgcgcagcga tcaggaaatg
      481 ctcgacgcaa tcctcgcccg cggcgcttcg cgggcgcggg ccctggcgat cccgacgctc
      541 gacaaggttt acacggcgct gggtctggtg cggggctgaa ggttttctcc gccccccgca
      601 cgcttcgtca tgctgatctt ggttcagcat ccatgacgcg aagtttcggt cagcgccgcg
      661 ccgtgggcct ggcgcggtgc gggtggcacg cccggcccgt ggatcctgaa acgggttcag
      721 gatgacggag tggtttgggg tcatggcgcg ctccggtctt gcctgctcgt catgctgaac
      781 ctgtttcagc atccttggcg caaggcttcg cttggcgctg cgcaggatgc cggggctcgc
      841 gacgggggga cggcatcggc ggaggccttc ctggtcagct tacggaaagg ttctggtggg
      901 cagggagcca ggcggggctg cgtttcactg ttgcgtggga gcgccgcttg atcctgtttc
      961 agcctgacat gcgtgagggg acctatggcc cctctccccg cgagcgcgcg atttcggcgc
     1021 tgctgtcgct ggcgctgtgc ctggcattgg cattcatgat cgtgcgcatg ggggcgatgg
     1081 ggccgagcct gccggcgaac ggctcgcggc tggttgcggt cgatgtaagc gcgcacgaat
     1141 ccgacaagcc ccggcaggcc cagcgcagcc cggcgcatca cgtggcggcg gcgagtgcca
     1201 cggctccggt gatcacccct cccccgccag tgccggccac cagccctgtt cccgcgctga
     1261 acctcattcc gctcacccgc gagcaactgg cctcggccga tatcggccgc atgcccaggc
     1321 gcggcccggc tgccggccct cccgcccccc aggatggcgg cgccgacggc gggggcagcg
     1381 gggacggccc cggcggtgcg cggctctatg ccgccgaatg ggtgcgcgag cccaccaatg
     1441 ccgaactggc aacctacatt ccgcacgagg ccgcggcgcc cggtgcctgg gcgacgattg
     1501 cctgccgcac gattgaccgg ttccatgtcg aggactgccg ggagatggag gaaagcccgc
     1561 ccggctcggg ccttgcgcgg gggctccggc aggctgcctg gcagtttctg gttcgcccgc
     1621 cccgggtgaa cggcaaggcg ctggtcggct cgtgggtgcg cattcgcttc gagttcagcc
     1681 acgccgccgg ctcgggctga tcgcgatcct gttcggcgca cttgcgccac ggctggccgc
     1741 gcggcattca agcgcagttc agtgcccgtg cgttagacca acacacaggt gcgccagccc
     1801 gcggcaggag agcggcgcgg attcgaaggg ataagaggca tgtcgcgcaa gaaattcgaa
     1861 tttaccgctc tgggcaaatc cttgaagctg gcggcagtgc cgctggtgct ggccagcctt
     1921 tcggcctgcg ccaccgggct cgacaccagc gtcagccgct tccagtcgca gatgccggcc
     1981 ccggccgggc agactttcgc cgtggtggcc gacaatccgg cgctggccgg ggggctcgag
     2041 tttgcgcagt acgcccggct cgtcgaaggg cagatggtgc ggctgggcta taccccgtcc
     2101 gacccagccc atgcgaccat gatcgtgcgg ttcgactatg gcgttgaccg aggccgggag
     2161 cgcatccgtt cgaccggcgc gggattccgg gatccgttct gggatccctg gtatggctat
     2221 cgcggcttcg gcggcggcta ctatggccgg ccctatcgct ggggcgccta tggcggctgg
     2281 ggcttcggct ggtatgaccc gtggttcgac aatgatgtgg agagctacac cgtctacacc
     2341 agcggcgtct cgctgaagat cgaccgcaag gtggacggtg tgcggctgtt cgagggcaag
     2401 gccgaggcgg tctccacctc caaccggctg cagtacctgg tgccgaacct ggtggaagcc
     2461 atgttcaccg gcttcccggg ccattcgggc gagacggtgc ggatcaccgt ggcgccggag
     2521 aacgcgaagc actgatcggg ccgatccgtt gatttgatcg aggcccttgc ccggttgaat
     2581 ggaggcgcgc aggagaaggg cgatgaaagc cgcccttttc ctgcgcatcg ccctgctgat
     2641 cgcgctgccg agcgccctgc tcgccgctcc gctcggcgcg caggcgacct ccccccgctg
     2701 gtcgaccgtg gcgctggccg acctgcacaa gtgggtggcc gccgcgcccg ccgatgccct
     2761 tcccgccccc gacgcgagcg cgctggaagc ggcggagcgg ctcggcgacg gggcggcggt
     2821 tgaccgcgcg gcggacgggc tggcgctgaa gctcgccacc atgcacctca ccggctgctg
     2881 cggggcgaac catgccggct ggcacatcgt cgacaccgac agcacggccg acctcccggc
     2941 gcggatcgcg gcggcggtga gcggcggaac gctcgacgcg tttttcgcgg ggctggcccc
     3001 gcagaacccg gactatgccg ccctgcgcgc ggcctatgcg gcggagcagg acccgggccg
     3061 caaggccacg ctggcgcgca acatggagcg gtggcggtgg ctgccgcgcg agccggggtc
     3121 acggtatctg ctggtcaata cggcggcctt cgaggtgcgc tactggtcgg gcgggaagct
     3181 ggtggaccgg cgggcggtga tcaacggcaa ggtttcctcg cccacgccga tcttcgcggc
     3241 gcgggtgact ggcattacct tcaatccgtg gtgggacatt ccgcccaata tcgtgcgcga
     3301 gggcatcggc aagctggcgc ggaccaaccc ggcggcggca cgggccaggg gctatgtgtg
     3361 gagcgggggc aagtttcgcc agcggcccgg gccgaccaat tcgctggggc tgatgaagct
     3421 ggtcatgccc aatccgttca acatctatct ccacgacacc ccgagcaaga acctgttcgc
     3481 gaagcccgtg cgcgccttca gccacggctg cgtgcgggtg agcgatgcgc ttgggtttgc
     3541 ctcggtgctg ctgggcgagg accgggcggc ggtggacgcg cgggtggcga gcggtgcaac
     3601 ggcgacggtc agcctgccgg cggcgatgcc ggtctatatc gcctatttca ccgccgggct
     3661 tgggccggac gggcaggtgg cgttctatcc cgacatctac gggcgggacg cggccatggg
     3721 ggacatgaag gacaacaagc cgttctgcgc ggcgtgatcc tgcccgcgag cggggaggat
     3781 cagagcgccg cgaccccgcc ggtggagccg aagccgccct cgccgcgggc ggtttcctcg
     3841 aggtgctcga cctccagcca gccagcccgc gtcaccgggg cgagcaccag ctgggccacc
     3901 cggtctcccc ggcggatcac aaacggctcg gcgccgtgat tgatcaggat gaccttgagt
     3961 tcgccgcgat agtccgaatc gatggtgccc ggcgtgttcg gcacggtgat gccgtgcttg
     4021 agggcgaggc cggagcgggg gcggacctgg atttcgaagt ggggcgggat ggcgacggca
     4081 aggccggtgg cgacggcgtg gcgggcgccg ggggcgatgg tgacgtcctc ggccgaaacc
     4141 acgtccatgc cggccgcgcc ttcggtggca taggcaggaa ggtccagccc tgcgccgtgg
     4201 ggcaggcgcc tgaccagcac gggcacggga tcattcggcg agggcattcg cgatcctttc
     4261 gacaagggcc gtggcgacca tgtccttggg catctcgggc aggttctcca ccccgtcggc
     4321 cgtgacgatg tgcacggtgt tggacttgcc gcccatcacc gacttgccct tgctgtcaaa
     4381 cgagacgtcg ttcgcgacga tccagtccgc ccccttgcgc ttgcgcttgt cccgggcgtg
     4441 ctcgatcacc ttgtcggtct cggcggcgaa accgatcagc agcctgggct tgagcgaggg
     4501 gctggagccc acgctggcga ggatatcggg gttttcggtg agcatcagca ccggcggggc
     4561 cgagccgcgt ttcttgagct tggacgtggc gacatccttg gtgcgccaat cggccacggc
     4621 agcgaccatg atcgcgacat cggccgggag ggcctccttg accgcggcct gcatctcgcg
     4681 cgcggattcg acatcgatcc ggtcaacccc cggcggggtg gcgaggttga ccggccccgc
     4741 gacgagcgtg acctgcgccc cggcgcgcgc ggcggcggcg gcgatggcga agccctgctg
     4801 gcccgacgaa cgattggcga tgtagcgcac cggatcgatc ggctcgtgcg tggggccggc
     4861 ggtgacgaga acgtggcggc cggagagcgg gccatcctcg ggccggacga aaggcgcagc
     4921 cggttcgagc ggcagctggc cgccgacgct gatgccgaac tggtcgcccg ggatcggctg
     4981 gggggcggac tggcgctcgt tgacgaggtg gttaagcgct tccggatcgg ttggcggggc
     5041 ggcgcggccc ttgcccttct tggccagcag ggcgcccgcc cccggcggag ggactgcggg
     5101 cgcttcaggt tcggcggccg gggcggagag cgcctcctct tcgcccaggg cctcgagggc
     5161 catggccttc ttcgcgccct tgccggcggc gcggccgatc agcgaggcgg agaggccgcc
     5221 gaggccgaca tccggaccgc tatcgccttc atcattggcg aaggtgggga gcggatcgag
     5281 cggcgtgaac ggctcgtcct ccggcgccga ccagccggcg ccatcgcgca tcggcagggc
     5341 ttcgggcccg gtgaaccacg aggcgatgag gcgccagacg gcatcgggtt cgggaaggcg
     5401 gccggggccg aattcgccgc aggccatggc gccctcgtcc gggtccatca cgaagacccc
     5461 ggcatcccgc aggctggcga cgttgcgctg ggtggcggcg tgctgccaca tgcgcacgtt
     5521 catcgcgggg agggcgagaa cgggcttgtc agtcgccagg aggagcgtgg tcgccagatc
     5581 gtcggcaatg ccggcggcca tcttggccat gagatcggcc gtggccgggc agaccgcgac
     5641 cagatcagcc tcgcggctga gctggatgtg gcccatctcc acctcgttcc tgagatccca
     5701 cagggtggtg tggacggggt tttccgagag ggccgccagg gccatgggcg ttacgaactt
     5761 ggcgccgcca tcggtcagca cgcaggttac cgagccgccg cccttgcgga tcatccgcac
     5821 gagttcgcac gacttgtagg cggcgatgcc gccgccgacg atcaggagga tgcgcgggcc
     5881 agccattggg tcatgttctc tgcacacctt ggcggccgag ggcaatcggg atttgccccg
     5941 gattggcggc acgccacagg tcgcgcaggg ttgcggggag gaaagcgagg ccgacgagca
     6001 gcgtgggcgc gacgaccatg ccccagtaga agttgtcgcc ccggcccgcg atcatgaagg
     6061 cgagaccata gccggcctgg agcaaggtgc cgaacaggcc ggcctgcccc ttccagccgg
     6121 accagccgag caggctggcg accaccagca cggcgccgac cgcgtggggc aggagcctga
     6181 ggttgctgga gagcaccagc atcgacagcc agccgctgag cccgcgcagg gtgatccacg
     6241 aggcggaggg cggatcggag ggcacgacca tccgggccac gaggtggaga tgaacggcca
     6301 gtgcggcgag gaacaggacg gcaaggccgg cccaggccag cccttcgcgc cagcgccgct
     6361 gccacagggc gaaggcggcc atgaggagca cgaagggcag cgcatgttcg cggatcgcaa
     6421 gggcgagcgc ggcggcgagc caggcgccga gccaccggcc ccgctcgggc cggtggaggc
     6481 cgaaggagag cgcaaggagc tggcccgccc agagttcgtg gagaacgaag aaatagcggg
     6541 tggtgccgat ggtcacgccg atcagcaggg cggccatggc caggctgcgc accggcaggg
     6601 cggcgggatc gccgccgagc ttgcgccacc aggccagcag cacgccaagc gccagcacga
     6661 ccgaaagcgg cgcaagcgcg cgagcgggca gcgcggcttc gatcacggcc agggtcggca
     6721 ggcgaaccgc gaagcccggg tgcagcggga agcggtgcct gcggtgttcc tcggcgatga
     6781 ccccatagta gccttcgccg tggcgaaggc gggcgatcac gtcgtcgtag agctggaggt
     6841 cctcgtcgcg ggtgcggacc tgcagcgtgg cggaagcggc agcgggccct cgggcaccgg
     6901 cggcggcatc attatcgcct gcgcggatcg gggtcagggc cgcgacggcc atgagggcca
     6961 gccacagcgc cagggccgcc aaggccgccc gccgcggcca ggcggcatag cgggtggtga
     7021 cgccaggctc agccaagcgc gggctccctt cgccgcgcca accccgccgc gcgccacagc
     7081 gatggcagcg cgatcggcag gaaagccgcg cccatgaaca gcagcggggt gatgagaatg
     7141 ccccaataga agttgttgtc gcgcccggcg atcgagaagg caaaggcata gccaagggtc
     7201 agcagggcgc cgaagttgcc catcggcgtg cgccagccgg tccagccgaa caggctcagc
     7261 accacgaaaa tgcccgcgac aggaaccggc acgagcgaga ggaaggtgga gttgttcacc
     7321 ttgtacatca gcccggcgat gcccttgagc acgaaccacg aggggctgac cggatcaccg
     7381 ggacggattt gggcctcggc gaggtgcagg tggaccatca gcgccgcacc gaacagggcg
     7441 atcagcgcga tccacgcggc cccttcgcgc cagcggcggt gccacagggc ccaggcggcc
     7501 atcagcatga cgaacggcag caccagctcg cgcacggcga gcgcggcggc agcggcgatc
     7561 caggcacccc cccagcggcc ctcatccggc cggtggaggc cgaaggacag ggcgatcaac
     7621 tgagcggccc agatttcgtg aaccatgttg tagcgcgccg acagtccgct ggcgatgccg
     7681 gccatcagca gggccagcgc cattggccgg cggtcttcgc cgcccgcctc gagcccgagc
     7741 cggcgggcat tggcgagcat catggcgacg aacagcatga cctgcagcgc gaccaggccg
     7801 accggtccga gcgcgacgga aaccatggcc agggtgggca ggcggaccgt gaggccgggc
     7861 gcgacggggt agttgttggc ccgctgctcc tcggtggccg cgacatagta attatctccg
     7921 cgagcaaggc gctggtagat caggcgatag agcctgaggt cgttatcgtc gcttttgaca
     7981 acgttgtggc ccgacgcatc ggtgaccgtg atcttgggcg gttcgggcgg cgggggcgaa
     8041 tagcccggag cgatcacggc cagcagcagg aacgcggcca gcaggccgag gaccacgcgg
     8101 gccatgaatt gcgaaaaacc cgcgtaacga tcgaagctgg gccgcgcctc tagcccggcc
     8161 agccgtgcgt catcagtatc cacgtaacca gtcccccgcc gatccctgca aggaggtatc
     8221 ccggccagct agcccgcata tggttaccat cgggttgacg gacggcctcc gcgccggacg
     8281 agcggccgcc ccagaccagc ccgatgtcgc gcagcggcgg ggcttcgggg gcgcctccct
     8341 tggcgggaaa gcgctcttcc acccggcgca ccagatcggg caggcgcagc agggtctgga
     8401 agtcttcccg cagcttttcg gcgatcgccg cctcgggccc gagttcgtcg cggatccacg
     8461 aacgcacgaa gggcgccgaa acgtcccaca tgttgatcgc gggatcgagc gcctgggcca
     8521 ggccttcgac catgaccatg gtcttctgca ggagcagcag gtggggctgg accgccatgt
     8581 cgaaatcgcg ggtgatggca aagagcccgt cgagcatctg gccgacagag agctcgctca
     8641 ccggacggcc acgcatcggt tcgcccacgg cgcgcagcgc ggtcgcgaac tcatctaccg
     8701 agtggtagct cggcacgtat tgcgcctcga agtggatttc ggcgacgcgg cggtaattgc
     8761 cggtggtcag gccatagagg atttccgcca gccactggcg cgcgcggcgg tcgatccgcc
     8821 ccatgatccc gaaatcgatg gcgacgatgg tgccgtccgg ccggacgaag agattgccct
     8881 ggtgcatgtc ggcatggaag aagccgacgc tgatcgcctg ggtgaggaag gcaagaacga
     8941 ggcggccggc caattcgggc aggtcgtgac cggcggcgcg cagggcggca aggtcgctca
     9001 tcttggcgcc gtcgacccat tcgatggtca ggaccttgcc gttggtgcgg tcccagtcga
     9061 tctcgggcac gtgatagccg gcgaagctct tcatcgcctc ggccagttcc gaggccgagg
     9121 cggcttcgcg gcgcaggtcg agctcgcgcg cggtccagcg cttcaagttc tcgaccacca
     9181 gccgcgggcg cagccgctcc gcctcgccgc cgagcgcctc gagatgggcc gcggcccatt
     9241 cgtaggtatc gatgtcgcgg gcgaacttcc tggcgatccc ggggcgcagg accttgaccg
     9301 cgacggtgcg gccatcggcg gtgaccgcgc ggtggacctg ggcgatcgag gcagcgccga
     9361 ccggttcgcg ctcgaccgag gcgaacaggg cttcaagcgg ctgctggaag ctcgcctcga
     9421 tctcggtgcg gatttcatcg aaggcaacgg gtggcaggct gtcctgcaag gtgagcaggt
     9481 tgtgggccgc ttcctcgccg acgaggtcgg gccgggtggc gagggtctgc ccgagcttga
     9541 tcgccgccgg gccgatggcc tggaaggcgc cggcatagtc gggaatgcgc ggctggcggg
     9601 tgccgaagcg ggcgagcttg cacaggcggc gcaccggggc gggcgtgttg aacccttcct
     9661 cgatgatgcg cagcgctccg tggcgcgcga ggatgcggcc ccacttgagc agccgcagga
     9721 tatgggtggt ggcggtggtc acccctcagg ctttccagcc cgaatgaatg gcgacgaggc
     9781 cccccagaat gggctcgacc ttcacgcggc tgaacccggc gtcgcggatc atctgcgcga
     9841 accggggcat gggcgggaag cggcggatgg attcgatgag gtagcggtag gactcggcgt
     9901 cgccggcgat catctggccc agtttcggca cgagatggtg cgaataggca tcataggctt
     9961 ccttgaagcc tggccattcg gtggtcgaga attcgaggca gaagaaccgg ccgccgaact
    10021 tgagcacgcg gtgggcttcg gcgagagccg catcgatgcg ggtcacgttg cggatgccaa
    10081 aggcgatcgt gtaggcatcg aaggcgcgat ccgggaaggt gagggtctcg gcgttctgcg
    10141 ggctccaggt gaggccctgg atggcctttt tcgcggcgcg gtccatgccg acggcgagca
    10201 tgtcctcgtt gatgtcggac acggtgacca gcgcgccgtg gcgggccatg cggaaggcga
    10261 tgtcgcccgt gccgccggcc atgtcgagga tggactcgcc cggccgcggc ttcacccggc
    10321 gaacgaactt gtccttccac aggcggtgaa gcccggcgct catcgcgtcg ttcatcacgt
    10381 cgtacttcct ggcgacgctg gagaagaccg cgccgacgcg ccgggtcttc tcctcggggg
    10441 agacctcttc gtagccgaag gaaacgtgat cgctggaaac ctgatcgttc atggcgcgtc
    10501 ctctagccgc gccgcaggca tggcgccaga gggactctcg ggccgccccc gcgccgccgc
    10561 gaatgcgccc agggcgatgc cgaccacgat cagcgccatg ccccacaggt gatacagatg
    10621 cagcacctcg cccagcagca gcgcggagag cactgcgccg aacagcggca ggagcgtgat
    10681 cgcctgtccc gccacgaccg gaccggtgcg cgccgcggcc gcattgaaca gaaaataggc
    10741 cgagagcgaa gccaacagcg cgacgtaggc aaaggcgccg aacaccccgg ggccccagtg
    10801 caccacctgc ccggagcgcc attcccacgc agcgaacggc gccagtgcga gggcggccac
    10861 ggcgaaggtc agggcaatca cggtgatcgg cg
//
LOCUS       DATNBV010000166         2223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_404909,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000166 DATNBV010000000
VERSION     DATNBV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404909"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..895
                     /gene="rlmB"
                     /locus_tag="VN627_03970"
     CDS             68..895
                     /gene="rlmB"
                     /locus_tag="VN627_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HXD02445.1"
                     /translation="MSDRDRKPPFRRGGGKPFEKGRKFARPHGHRDRDSSPDGPVILY
                     GWHTVSAALANPERGIRKLFLTENAARRLADENIDTRVPPEIVRPSAIDQRLGPDAVH
                     QGLLAEADPLPSPDIDTLAQEGIVLVLDQITDPHNVGAILRSAAAFAVKAIVTTARHS
                     PEATGVLAKSASGALELVPLVTVQNLARALNELNDRGFMTVGLDSEGTEDLGAVALQQ
                     PLALVLGAEGKGLRQLTRETCRVVARLDMPGEIKSLNVSNAAVLALYIGASRLGLMK"
     gene            complement(951..1328)
                     /locus_tag="VN627_03975"
     CDS             complement(951..1328)
                     /locus_tag="VN627_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02446.1"
                     /translation="MRQILKGLIAAVAVMAAAPAMACGYAPCAQPAYVAPVVGYGCNT
                     CGGWVHERLPDPEQQYYYVNQGPTYTGPGNWAPRRVYREGAISGYGYGYSRQYRVHRY
                     YHRHHHGYAARYHHSQRVLRRYY"
     gene            complement(1422..1856)
                     /locus_tag="VN627_03980"
     CDS             complement(1422..1856)
                     /locus_tag="VN627_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02447.1"
                     /translation="MALALSIAMTSTASAGGGFDIVIPGRPGVPVIINGIDASYTVVE
                     GEWGLGQNNQVQPTIYGGRYIDPVPHVGRYYPSAGRMPGYGRLEIEPPANRRLPQPAE
                     SYHQSWSAQSAPLPAQSNVPVNPPEIIYAPDYDRRRPGHLPR"
     gene            1970..>2223
                     /locus_tag="VN627_03985"
     CDS             1970..>2223
                     /locus_tag="VN627_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02448.1"
                     /translation="MDRRSIARDGAIQTLTPSRSPAGGSVRRPGGPLDCHLRGNIAPS
                     HLSRSLRTPAEMPEMTTKTPAKNVLWIMCDQLRYDYLGCTG"
BASE COUNT          409 a          719 c          709 g          386 t
ORIGIN      
        1 agcccctaaa aacggctctt ggggtgccca aattgccata ttcggggacc cgtgacacaa
       61 gctccccatg agcgatcgcg accgaaaacc gccgttccgc cgtggcggcg gcaaaccctt
      121 cgaaaaaggg cggaaattcg cccgtccgca cggtcaccgg gaccgggatt cgagcccgga
      181 cgggccggtg attctctatg gctggcacac ggtttcggcc gccctggcca acccggaacg
      241 gggaattcga aagctgttcc tgaccgaaaa cgccgcaagg cgcctggccg acgagaacat
      301 cgacacccgc gttcccccgg agatcgtccg ccccagcgcg atcgaccagc ggctcgggcc
      361 cgacgccgtg caccaggggc tattggcgga ggccgatccc ctgccctcgc ccgatatcga
      421 tacgctggcc caggaaggca tcgtgctggt gctcgaccag atcaccgatc cgcacaatgt
      481 cggcgccatc ctgcgctcgg cggcggcgtt cgccgtgaag gcgatcgtca ccaccgcccg
      541 ccacagcccg gaggccaccg gcgtgctggc caaatccgcc tccggcgcgc tggaactggt
      601 gccgctggtc acggtgcaaa acctcgcccg tgcgctcaac gaattgaacg accgcggctt
      661 catgacggtc gggctcgaca gcgagggaac tgaggacctc ggcgcggtgg cgctgcagca
      721 gccgctcgcg ctggtgctgg gcgccgaggg caagggcctg cggcaactga cgcgcgagac
      781 ttgccgcgtg gtggcgcggc tcgacatgcc cggcgagatc aagagcctga acgtctcgaa
      841 cgccgcagtg ctggcgcttt acatcggcgc gagccgactg gggcttatga aataaaggac
      901 gcccgctcgc ggaagcgaac gggcgtcgaa gttttcaaac taccgttgga tcagtagtag
      961 cggcgcagca cgcgctggct gtggtgatag cgcgccgcgt aaccgtgatg gtgccggtga
     1021 taatagcggt gcacgcgata ctgccggctg tagccatagc cgtagccgga gatcgcgcct
     1081 tcgcgataga cccgacgcgg cgcccaattg ccgggaccgg tataggtcgg gccctggttg
     1141 acgtagtagt actgctgctc cggatcgggc agccgctcgt gaacccagcc gccgcaggta
     1201 ttgcagccgt atcccacgac gggcgcgaca taggccggct gtgcgcaagg ggcgtaaccg
     1261 caggccatcg cgggcgctgc ggccatgacg gcgacagcgg cgatcaatcc tttaagtatc
     1321 tgacgcatta ctctctcctg ttcgtatttt cgttggcttt agagtttctt gtttgagcat
     1381 gatctttcgg aaaaccggtg cccacttttc cggatcatgc tctaacgtgg aaggtggccc
     1441 ggcctgcgcc ggtcatagtc cggcgcgtag atgatctcgg gcggattgac ggggacgttc
     1501 gattgcgccg gcagcggtgc cgactgcgcc gaccaggatt gatggtagct ctcggccggc
     1561 tgcggcaatc tgcggttcgc cggcggctcg atttccagcc gcccgtaacc gggcatgcgg
     1621 ccggcgctcg gataataacg gccgacgtgc ggcaccggat cgatatagcg gccgccatag
     1681 atggtcggct ggacctggtt gttttgcccc agaccccact cgccctcgac cacggtatag
     1741 gaggcatcga tgccgttaat gatgacggga accccgggtc ggcccggaat cacgatgtcg
     1801 aagccaccgc cggcagaggc cgtcgacgtc atcgcgatcg aaagtgccag tgccacgcca
     1861 atgcgcatcg cttgggttcc cgttctcctc gaggtcccaa tttaatccaa cgcgcgattt
     1921 caggagttaa ggcggccggc caaactgccg ataaggctaa cgcgccagca tggatcgccg
     1981 ttcaattgcg agggatggcg ctatccaaac gttaacgccg agccggagtc cggccggggg
     2041 atcggtacgc cgtcccggtg gcccgctcga ttgtcatttg cgcggcaata tcgcaccatc
     2101 ccacctctcc cgctctctcc gaactccagc cgaaatgcct gaaatgacga ccaaaacgcc
     2161 tgcgaagaac gtgctctgga tcatgtgcga ccagcttcgc tacgactatc tcggctgcac
     2221 cgg
//
LOCUS       DATNBV010000167         6012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_404960,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000167 DATNBV010000000
VERSION     DATNBV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404960"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VN627_03990"
     CDS             <1..845
                     /locus_tag="VN627_03990"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HXD02449.1"
                     /translation="EVIGDPIAQSKSPAIHGFWLGELGIAADYRACHVTAAGLADYIA
                     ARRGDPAWRGCNVTMPHKQAVMGLLDAIDPLAARVGAVNTVVPGADGRLTGYNTDVPG
                     FLEPLEPLLAQTHLFRMARVIGTGGAARAIIAGLAGHGFVLVLAGRNPDKARALLDEL
                     DPTGEHHAIDLAHFAEPTDFAFDDREGLCDLVVNASLLGMTGQPPLAFDWSHAPPGSL
                     AYDIVTSPLDTRFLKDARAAGFRTIDGLAMLIGQADYAFAHFFGRRPPRERDSELRRR
                     ITGG"
     gene            838..1440
                     /gene="coaE"
                     /locus_tag="VN627_03995"
     CDS             838..1440
                     /gene="coaE"
                     /locus_tag="VN627_03995"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165732.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HXD02450.1"
                     /translation="MDEARRPFILGLTGSIGMGKSAVAAMFHGLGVPVFDADAAVHEL
                     QGPGGELVQAIEAAFPGTTGAGGVDRQELGPMVLGQPEQLARLEALIHPAVGAMRAAF
                     LAENADKPLIVFDIPLLFEKGGQGVDAVVVVSASPEAQRARVLARPGMTEEKFAQILK
                     LQVPDAEKRARADFVIDTGTSLAETRHAVQRLVHQLTGRD"
     gene            1489..2184
                     /gene="dnaQ"
                     /locus_tag="VN627_04000"
     CDS             1489..2184
                     /gene="dnaQ"
                     /locus_tag="VN627_04000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HXD02451.1"
                     /translation="MREIIFDTETTGLDPLKGDRMVEIGCIELVNRVPTGQVFHAYFN
                     PCRDMPAEAEAVHGLSAAFLADKPLFGERCAELLEFIGDAPLVAHNAGFDFAFLNFEL
                     AQCGLEPVCHSRMVDTVKIARVRHPGAKLSLDALCTRYGIDRSHRTRHGALLDAELLA
                     QVYVELNGGRQIGLELAGARSSDVVAEVRLVHRTRVYREPRPHGPTEAELAAHAAFLE
                     GVKAPLWNEGAAP"
     gene            2209..2775
                     /gene="raiA"
                     /locus_tag="VN627_04005"
     CDS             2209..2775
                     /gene="raiA"
                     /locus_tag="VN627_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HXD02452.1"
                     /translation="MDIRVAGHQVETGEALQSHAAERLTTIVEKYFNRGLSSQVTFNR
                     GPAASFRCDIVTHVMQGLILKGAAFAPDAHVALDQAAEKIDKQLRRYKRRLKDHHEQA
                     SHAARAEEAAYVVFAEPEPEAEEIVVDAPLVIAETRVDVPEATVSDAVMMLDLRNTNA
                     LLFKNAGTGRHNMVYRRGDGSIGWVEPS"
     gene            2916..3377
                     /locus_tag="VN627_04010"
     CDS             2916..3377
                     /locus_tag="VN627_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXD02453.1"
                     /translation="MTALFTLVPEAVGTIRADDKPAIMRQLAGRFAEVYGLEASHVEE
                     RIEERERLGSTGFGRGVAIPHARIEGLSRPVAVFLRLEAPVAFDAADGMPVDLVFGLL
                     SPEHAGAAHLHALAAISRMMRDERMHQALSEAPGAEALYGLLCNVIDRTAA"
     gene            3352..3864
                     /locus_tag="VN627_04015"
     CDS             3352..3864
                     /locus_tag="VN627_04015"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXD02454.1"
                     /translation="MSSTVPLPDQADPEGVGPEVAGAALHWRALERLYAAAPINRLFN
                     SELTITGEGRARIAFMVDERVHHAAGAAHGTIYFKMIDDAAFYAANTLVTDRFLLTTS
                     MNLHFVRPIKRGPVVAEGHWVSGRRRVLVAEARLLDAEGEEIGRGTGTFMRSRIPLSG
                     LPGYHPAAGT"
     gene            3882..4214
                     /locus_tag="VN627_04020"
     CDS             3882..4214
                     /locus_tag="VN627_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HXD02455.1"
                     /translation="MAEPEARLPTHIEVGALLRRVQQEGGFATVIARGEADAGTLMVV
                     LADRNAPPRAFERMPQMDGTRAWTLSRAADPAEPHAFSEWLERRHGQDKDLWIIELDI
                     PQGERFIP"
     gene            4554..5138
                     /locus_tag="VN627_04025"
     CDS             4554..5138
                     /locus_tag="VN627_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HXD02456.1"
                     /translation="MTPQAVAAASAPDNLPGSSSATPQTNPGDADLRPAREEKLPPAL
                     QTAPEAESRVSLAPAAATLSRAAALGSIEMTRELECLAGAIYFEAKSESLEGQLAVGR
                     VVINRSKSGRFPSSYCGVVYQRSQFSFIRGGQMPAIDHSSRMWQNAVAVAKIADANAY
                     PSAAEGALFFHAARVSPNWRLTKVARVDNHVFYR"
     gene            complement(5289..6011)
                     /gene="xth"
                     /locus_tag="VN627_04030"
     CDS             complement(5289..6011)
                     /gene="xth"
                     /locus_tag="VN627_04030"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXD02457.1"
                     /translation="MLEWLEETRPAVACLQEIKTQDEGFPAAEFEKIGYGAIWHGQKG
                     FNGVAMLADGAQPVEIQRGLAGDPEDVHSRYLEADVFGLRIAGIYLPNGNPVPGPKFD
                     YKLRWMERLRARMAEIAAEEVPAVVMGDFNVIPEDKDVWSPRAMAADALMQPESRDAY
                     ARLLGDGWTDAIDVLNPRGGVWTFWDYQAGAWQRDHGFRIDQALLSPELADRLVAAGV
                     DKEHRGREKASDHAPVWVEVRG"
BASE COUNT          952 a         2019 c         2052 g          989 t
ORIGIN      
        1 ccgaagtgat tggcgatccg atcgcgcaat cgaagtcgcc ggcgatccac gggttctggc
       61 tgggcgagct ggggatcgcg gccgattacc gcgcctgcca cgtcactgcg gcggggctgg
      121 ccgactatat cgcggcgcgg cggggcgatc ctgcctggcg cgggtgcaac gtgaccatgc
      181 cgcacaagca ggcggtgatg gggctgctcg atgcgatcga cccgctggcg gcgcgggtcg
      241 gtgcggtcaa caccgtcgtg ccgggggcgg acgggcggtt gaccggctac aacaccgacg
      301 tgccgggatt tctcgagccg ctggaaccgc tgctggcgca gactcacctg ttccgcatgg
      361 cgcgggtgat cgggaccgga ggggcggcgc gggcgatcat cgcggggctc gccgggcacg
      421 gcttcgtgct ggtgctggcc gggcgcaatc ccgacaaggc gcgggccctg ctcgacgagc
      481 tagaccccac cggcgagcac catgcaatcg accttgccca tttcgccgag cccaccgact
      541 tcgccttcga tgaccgcgaa gggctttgcg acctggtggt caatgcgagc ctgctgggga
      601 tgaccggcca gccgccgctc gccttcgact ggagccatgc cccgccgggg agcctggcct
      661 atgatatcgt cacatcgccg ctcgacaccc ggttcctcaa ggacgcgcgg gcggcgggct
      721 tccgcacgat cgacgggctc gccatgctga tcgggcaggc agactatgcc ttcgcccact
      781 tcttcggccg ccgcccgccg cgcgagcggg acagcgagct caggcggaga atcaccggtg
      841 gatgaggcaa gacgcccgtt cattctgggc ctgaccggct cgatcggcat gggcaagtcg
      901 gcggtggcgg cgatgttcca tggccttggc gtgccggtgt tcgacgccga cgccgcggtg
      961 cacgaactgc aagggccggg cggggaactg gtccaggcga tcgaagcggc gttcccgggg
     1021 acgaccgggg cgggcggggt cgaccggcag gagctggggc cgatggtgct cggccagccg
     1081 gagcagctgg cccggctgga ggccctgatc caccccgccg tgggcgcgat gcgcgcggcg
     1141 tttctggccg agaatgccga caagccgttg atagtgtttg atattcccct gctgttcgag
     1201 aagggcgggc aaggcgtcga cgcggtggtg gtggtctcgg cctcgccgga ggcgcagcgc
     1261 gcgcgggtgc tggcccgacc gggaatgacc gaggagaaat tcgcccagat cctgaagctg
     1321 caagtgcccg acgcggagaa gcgcgcccgg gccgacttcg tgatcgatac cggaacttcg
     1381 ctggccgaaa cgcgccatgc ggtgcagcgg ctggttcacc aattgacggg gcgcgactga
     1441 cacaatcgag cttgcacccg ggccgttgca ggccgataag ttcaaacgat gcgtgagatt
     1501 attttcgaca ccgaaaccac cggtctcgat ccgctcaagg gcgatcgcat ggtggaaatc
     1561 ggctgcatcg agctggtgaa ccgggtgccc acgggccagg tcttccacgc ctatttcaat
     1621 ccctgccgcg acatgccggc cgaggccgag gcggtccacg gcctgtccgc cgccttcctc
     1681 gccgacaagc cgctgttcgg cgaacgctgc gcggaactcc tcgaattcat cggcgatgcc
     1741 ccgctggtgg cgcacaacgc cggattcgac ttcgcctttc tcaattttga gctggcccaa
     1801 tgcgggctcg agccggtgtg ccacagccgg atggtggata cggtcaagat cgcgcgggtc
     1861 cgccatcccg gggccaagct cagcctcgac gcgctgtgca cccgctacgg gatcgaccgc
     1921 agccaccgca cccgccacgg cgcgctgctc gacgccgaac tgctggccca ggtctatgtc
     1981 gagctgaacg gcggccggca gatcggcctc gaactggccg gcgcgcgcag ttccgacgtg
     2041 gtcgccgagg tccggctggt gcaccgcacc cgcgtctacc gcgagccgcg cccgcatggg
     2101 ccgaccgaag cggaactggc cgcccatgcc gcgttccttg aaggcgtgaa agcgccgctc
     2161 tggaacgagg gggcggcccc ctgaaatgtt agaaggagga gaatgattat ggacattcgc
     2221 gttgctggtc accaggtcga aaccggcgaa gccctccaga gccacgctgc cgaacggctt
     2281 accacgattg tcgagaagta cttcaaccgc ggcttgtcca gtcaggtgac cttcaatcgc
     2341 ggcccggccg cctcgttccg ctgcgatatc gtcacgcacg tgatgcaggg gctgatcctc
     2401 aagggggcgg ccttcgcgcc cgatgcccac gttgcgctgg atcaggccgc cgagaagatc
     2461 gacaagcagc tgcgccgtta caagcgccgc ctcaaggacc atcacgaaca ggccagccac
     2521 gctgcccgcg ccgaagaggc cgcctatgtg gtcttcgcgg agccggagcc cgaggccgag
     2581 gagatcgtcg tcgacgcccc gctggtcatt gccgagaccc gggtcgatgt tcccgaggcg
     2641 accgtgtccg acgcggtgat gatgctcgac ctcaggaata cgaatgccct gctgttcaaa
     2701 aatgctggca ccggcaggca taatatggtc taccgccgcg gcgatgggtc gatcggctgg
     2761 gtcgaaccgt cctgacgcgc aagatttctc cggcggagaa cctgcgcgcc acttccggcg
     2821 tttgactttg agtagtccgg tgtccgggct agatgacgcc atccgtcagc cccgccaggg
     2881 gccgccggat ggcccgacag gcgatacgaa cccagatgac cgctctcttc acgctcgttc
     2941 ccgaggccgt cggcaccatc cgcgccgatg acaagccggc gatcatgcgc cagctggccg
     3001 gccgcttcgc cgaggtctac ggcctcgagg cgagccacgt ggaagaacgg atcgaggagc
     3061 gcgagcggct cggcagcacc gggttcgggc ggggtgtcgc cattccccat gcccggatcg
     3121 aggggctgag ccgcccggtc gcggtgtttt tgcggctcga ggctccggtt gccttcgacg
     3181 cggcggacgg gatgccggtc gatctcgtgt tcggcctgct gtcacccgaa catgctggcg
     3241 cagcccacct gcatgccctc gccgcgatct cgcgcatgat gcgcgacgag cggatgcacc
     3301 aggcgctgag cgaggcgccc ggcgccgagg cgctctacgg cctgctctgc aatgtcatcg
     3361 accgtaccgc tgcctgacca ggccgacccg gaaggggtcg ggccggaggt cgctggcgcg
     3421 gcgctgcact ggcgcgcgct cgaacggctc tatgcggccg caccgatcaa ccggctgttc
     3481 aattccgagc tgaccatcac cggcgagggc cgggcacgga tcgccttcat ggtcgacgag
     3541 cgggtccatc atgccgccgg tgcggcgcac ggcacgatct atttcaagat gatcgacgat
     3601 gccgctttct acgcggccaa taccctggtg accgaccgct tcctgctgac cacctcgatg
     3661 aacctccact tcgttcgccc gatcaagcgc gggccggtgg tagccgaagg gcactgggtc
     3721 agcgggcgcc gccgggtgct ggtcgccgag gcgcggctgc tcgatgccga gggcgaggag
     3781 atcggccgcg gcaccggcac tttcatgcgc agccggatcc cgctctcggg cctgcccggc
     3841 taccatccgg cggccgggac ctgatttcgt ggctgggcgg aatggctgaa ccggaagcgc
     3901 ggctgccgac ccatatcgag gtgggcgcgc tgctgcggcg ggttcagcag gagggcggct
     3961 ttgccacggt gattgcccgc ggcgaggccg acgcggggac gctcatggtc gtcctggcag
     4021 accgcaatgc cccgccccgg gccttcgagc gcatgccgca gatggatgga acgcgcgcct
     4081 ggacactgtc ccgcgcggcc gatcctgccg agccccatgc cttctccgaa tggctcgaaa
     4141 ggcgccatgg ccaggacaag gacctgtgga tcattgaact ggatatcccg caaggcgaac
     4201 ggttcatccc atagtattcg tgcgcaggtt gactctgttg cgctgcacaa tcaagggcgc
     4261 acctccactt acgcgcaggc ggtctgacgg agcaagcaag ctccgcggct ggcacgcaga
     4321 cgggtggcag ccggcgagtt accggtggtc agggagggtc gcacgcttgt gcaaccttgg
     4381 cttgtccatc ccctcgtgca ggtgtgccaa ccgccgataa agacgggttg atgagtttca
     4441 aaagcaagtt ttccagtgcg ttcgccgcga cactggctgt tatgtttacg attgttgccg
     4501 ctgacggttc cggcgcggca gcccagaatc ggaatcccca gatcaccgga cagatgacgc
     4561 cgcaggccgt ggccgccgct tctgctcccg acaatcttcc cggcagctct tccgccacgc
     4621 cccaaaccaa tcccggtgac gccgacctgc gtccggcccg cgaggaaaag ctcccccccg
     4681 ccctgcagac cgcgcccgag gccgaaagcc gcgtgagcct ggcccccgcc gccgccaccc
     4741 tgtcgcgcgc tgccgcgctc ggcagcatcg agatgacgcg cgagctggaa tgcctggccg
     4801 gcgcgatcta cttcgaggcc aagagcgaga gccttgaagg ccaactcgcg gtgggccgcg
     4861 tggtgatcaa ccgctcgaag tcgggccgct tcccttcgtc ctattgcggc gtggtctacc
     4921 agcgctcgca gttctcgttc atccgcggcg ggcagatgcc ggcgatcgat cactcctcgc
     4981 gcatgtggca gaacgccgtg gccgtggcca agatcgccga cgccaatgcc tatccgagcg
     5041 cggccgaagg cgccctgttc ttccacgccg ctcgcgtctc gccgaactgg cgcctgacca
     5101 aggtcgcccg cgtggacaac catgtgttct accgctgatc ggttgatcgg ctgatcggcg
     5161 cacccgtcat gggggcaagt tctacgcccg ccttcccaac cccgggaagg cgggcgtttt
     5221 ttacgcaaca ggtttggcta caagtctaca ccggggcgac tgtgtaattc tcgaccgcgc
     5281 gggcggaact aaccgcgcac ttccacccac accggcgcgt gatcgctggc cttttcccgg
     5341 ccgcgatgct ccttgtcgac gccggcggcg acgaggcggt cggcgagttc ggggctgagc
     5401 agtgcttggt cgatgcggaa gccgtggtcg cgctgccagg cgccggcctg gtagtcccag
     5461 aaggtccaga cgccgccccg ggggttgagc acgtcgatcg cgtcggtcca gccgtcgccg
     5521 agcaggcggg cataggcatc gcgcgattcg ggttgcatca gggcatcggc cgccatcgcg
     5581 cggggggacc agacgtcctt gtcctccggg atgacgttga aatcgcccat gaccacggcg
     5641 ggcacttcct cggccgcaat ctcggccatg cgggcgcgca ggcgttccat ccacctgagc
     5701 ttgtagtcga acttggggcc ggggacgggg ttgccgttgg ggaggtagat cccggctatg
     5761 cgcaggccga agacatcggc ttccaggtag cgcgaatgga cgtcttccgg atcgccggcc
     5821 aggccgcgct ggatctcgac gggttgcgca ccgtcggcca gcatggccac gccgttgaag
     5881 cccttctgcc cgtgccagat cgcgccgtag ccgatcttct cgaactcggc ggcggggaag
     5941 ccttcgtcct gggtcttgat ttcctgcagg caggcgaccg cggggcgggt ttcctccagc
     6001 cattccagca gg
//
LOCUS       DATNBV010000168         2048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_405572,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000168 DATNBV010000000
VERSION     DATNBV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405572"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(594..1184)
                     /locus_tag="VN627_04035"
     CDS             complement(594..1184)
                     /locus_tag="VN627_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HXD02458.1"
                     /translation="MPRHLVQSPAPLRRAILAAALAGALTGLAGCTPSGLAERPAVGS
                     FAYRIGPGDRLRIATYGEDRLTGEFTVTAQGAIAFPLLGEVAAEGRTVSEFTALLQRR
                     LGSEYMRNPQVSVEMVNFRPVYILGEVTRPGEFPYGERLSVYALVAKAGGFTYRANQG
                     YALIRSENEPGERAVRLTSATAVQPGDTIRIPERTF"
     gene            1382..1942
                     /locus_tag="VN627_04040"
     CDS             1382..1942
                     /locus_tag="VN627_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HXD02459.1"
                     /translation="MTTPRFLAGRLLLAMPGMFDPRFARAVIAMCIHDENGAMGVGVG
                     RLHEGLRFRAVLEHSGIDPGEAPDAPVHVGGPVEQQRGFVLHSTDWGGNGTVEVAPLC
                     ALSFSVDVLRAIAEGRGPSRWLFALGYAGWGEHQLEDEMREHGWHAAAAHPEILFDTP
                     VADRWQAAWKAEGIDTALLASETGHA"
BASE COUNT          329 a          690 c          723 g          306 t
ORIGIN      
        1 gcaagaaccg cgccgggccg ggacagaccc ggtgatgcca gcggcggcag ggcgatgtcc
       61 caagggcttg cgaaggggct cgtcctccct tccaattcaa ggacacgagc gccagaggcg
      121 gccggagcaa tcgcgcttgg agaaccggcc acatgccagg cagcgcccgg caggcccctg
      181 accgaggcag cagccattgc ccaacccggg gcgctggccg tccgccaccc ggcaccggct
      241 gagccccccg aaagcggccc agccgcgaca ggtccgccgc gatcactccc aatcgctgcc
      301 cccagaggca ttgcgatcgc cgcgaccggt gcgactgccc atccggtgaa tgcgcccgga
      361 tgtgccgccg tgaccggtcc gcgcgccgtc tgtgcgggca ggaagcggga atcgagctgg
      421 ctggtattga gcatcggcgg ccccggcgaa agctcctgca gggcaggagg gcatgggtag
      481 gatagcaagc cctgccgcgg gtctgaccgg gacttctacc tagcccgccc cggagacatc
      541 tccggatcgg aggcaaatgc ccggtcagcc ccggcgacgg cgagcccggc tgatcagaaa
      601 gtgcgctccg gtatgcggat cgtatcgccc ggctggacgg ccgtggcact ggtcaggcga
      661 acggcgcgtt ccccgggttc gttttcgctg cggatcaggg catagccctg gttcgcgcgg
      721 taggtaaacc cgccggcctt ggccaccagg gcatagacgc tgagccgttc cccataaggg
      781 aactcccccg ggcgggtgac ttcgcccagg atgtagaccg ggcggaaatt gaccatttcc
      841 accgatacct gcgggttgcg catgtattcg ctgccgagac ggcgctggag cagggcggtg
      901 aattcggaca cggtgcggcc ttcggcggca acttcaccaa gcagcggaaa ggcgatggcg
      961 ccctgcgcgg tgacggtgaa ttccccggtc aggcgatctt ctccgtaggt ggcgatgcgc
     1021 aggcgatccc cggggccgat ccggtaggcg aagctgccga ccgccgggcg ttccgccagg
     1081 cctgacggcg tacagccggc aagcccggtc agagccccgg ccaaagcggc ggcaaggatg
     1141 gcccggcgga gcggagcggg cgattggacg agatggcgag gcatgtgaag ctggttcccc
     1201 ccgaagtggt cacctgcgta tttaacccga tctgcgcccc gcttgtggcc aagtgcctcc
     1261 acttcggtgc aaacccacat cgccgctgca actgcgctgc aagcggcgag cagcacccgg
     1321 cacgcgccgg gaccggcgta aactggtttc ctgccagcgt ccgatggcct aggctgggcc
     1381 gatgaccacg ccgcgttttc tggccgggcg gctgctgctg gccatgcctg gaatgttcga
     1441 tccgcgcttt gcccgcgccg tcattgccat gtgcatccat gacgagaacg gtgccatggg
     1501 cgtcggcgtt ggacggctgc acgaaggcct gcgcttccgc gcggtgctcg agcattccgg
     1561 gatcgatccg ggcgaggcgc ccgatgcgcc ggtccatgtc ggcgggccgg tggaacagca
     1621 gcgcggcttc gtgctccatt cgaccgactg gggcggcaac ggcacggtcg aggtggcccc
     1681 gctctgcgcg ctgtcgttct cggtcgacgt gctgcgcgcg attgccgagg ggcgcggccc
     1741 atcgcgctgg ctgttcgcgc tcggctatgc cgggtggggc gagcaccagt tggaagatga
     1801 gatgcgcgag catggctggc atgccgccgc cgcgcacccc gaaatcctgt tcgatacgcc
     1861 ggtggccgat cggtggcagg ccgcgtggaa ggccgaaggg atcgacaccg ccctgctggc
     1921 aagcgaaacc ggccacgcct gagggcagcg ccggattggc catcacgggc atgtgcatgc
     1981 cccctcggca cttgaccatc ccggccggat cccgacatct gggcccaaga ggatcatccc
     2041 atgcgcgc
//
LOCUS       DATNBV010000169        15130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3289745,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000169 DATNBV010000000
VERSION     DATNBV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 15130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15130
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3289745"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VN627_04045"
     CDS             complement(<1..266)
                     /locus_tag="VN627_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HXD02460.1"
                     /translation="MWGKTAILIAMALGAAAAPARADTVVDSDHVLGGGSANLPPYLQ
                     CVPYARAVSGVLIFGDARTWWDQADGRYARGTTPKVGAVMTFKP"
     gene            407..1792
                     /gene="der"
                     /locus_tag="VN627_04050"
     CDS             407..1792
                     /gene="der"
                     /locus_tag="VN627_04050"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HXD02461.1"
                     /translation="MLPQVIIIGRPNVGKSTLFNRLVGKKLALVDDQPGVTRDRRFGE
                     AHLLGLDFTIVDTAGWEDEDAASLPGRMRQQTEAALKGADVALFVVDARAGITPLDEE
                     IARHLRSSTVPIVLLANKAEGRAGDPGLYEAFSLGFGEPIAFSAEHGQGLADLFEALL
                     PHVDDKQREHEEQVAADEAEDDYDPQAILKLAIVGRPNAGKSTLINALLREDRLLTGP
                     EAGITRDSIAVDWTWTDPKTKAERPVRLIDTAGMRKKAQVVEKLEKLSVADARHAIDF
                     AEVVVLLLDATRGLEHQDLKIASMVLEEGRALIIAINKWDVAEDASGLFNGVRAALDE
                     GLAQVRGVPLLSVSARTGKGLDTLIQAAFDIRDAWSKRVPTAALNRWFDDALAANPPP
                     APGGRRIKLRYITQAKTRPPGFVLFGTRLDQLPESYRRYLVNGIRRELGFDAVPIRLT
                     LRSPKNPFAKK"
     gene            complement(1806..1976)
                     /locus_tag="VN627_04055"
     CDS             complement(1806..1976)
                     /locus_tag="VN627_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02462.1"
                     /translation="MANSIRSAPVRLLRRRPQPDSLRLPHPSWRLALGSAPGGEDQRL
                     ASASEFWARLGL"
     gene            complement(2114..3424)
                     /locus_tag="VN627_04060"
     CDS             complement(2114..3424)
                     /locus_tag="VN627_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HXD02463.1"
                     /translation="MDAAPGLLPHAGEKGRLASLDLLRGVAVLGILAINIGGFAGPIA
                     ATLSPNQLEPGTFADEAAWAFALVVFEGKMRALFTLLFGASLVLFMERADRRGLNGER
                     LQFRRLGWLALAGYLHFLLLWWGDILFTYALAGFAALAFRQAPPRALGAGALALFAGW
                     HLLLAVASGPDVAREERVLSGHASPGEAVAYALVRDEVRADTQREVARDRMGFAAQVA
                     AKWREEPSEPVSSALAGMGETLPLMLLGMALYRGGFYSGQVRRRTLRRLAVGGIAGGG
                     AATLAFLGWAWPRQFPPDLMQAALACGLALPHLAMALGYGAALLLAVPRFAPTWLGQR
                     LSAAGRMAFTNYIAMTAVMCALFQGWGLGLIGTMPERWQWLLVLAGWALMLGWSQPWL
                     ARFRHGPLEWLWRSATWGRWQPLRRQVRVAASQENLSRQSPANW"
     gene            complement(3429..4550)
                     /locus_tag="VN627_04065"
     CDS             complement(3429..4550)
                     /locus_tag="VN627_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXD02464.1"
                     /translation="MRGIRIWPAAALVAMAALPAQADVKAGADAWSRGDYAGAVAQWQ
                     EPARSGDPDAEFNLGQAYRLGRGVPRDLGRAEELFGKAAAQGHVQAADNYGLLMFDRG
                     ARAQAMPYVKAASDRGDPRAQYLMAIAHFNGDLAAKDWVRAYALMTLARSAGLPQAAP
                     AIQQMDSFIPLAERQQGASLAPQLAQQAETTRNTQIASNDLGEAPAAAKPLAADAPPP
                     VGVPEPGGAATAGADYARPAGAQAPPLPVPGAKPVAVRPAVGAAPKPVGAAAPALKPA
                     PAKPAVAVALAPKPRPAAAVTGGIWRVQLGAFGVAANADALWARASARPELAGHRRID
                     LRAGKVSKLQAGGFASPGAAASACAGLTRAGFTCLVTQD"
     gene            4814..5536
                     /locus_tag="VN627_04070"
     CDS             4814..5536
                     /locus_tag="VN627_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXD02465.1"
                     /translation="MRVLAIASQKGGSGKTTLSGHLAVQAQRAGAGPVVLIDIDPQGS
                     LADWWNERADDFPAFAQTTVARLAADLQVLRQQGFKLAVIDTPPAITMAIQSVIGVAE
                     LIVVPTRPSPHDLRAVGATVDLCERAGKPLLFVVNGATPKARITSEAAVALSQHGTVA
                     PVTLHHRTDFAASMIDGRTVMEVDPNGRSSMEVAALWGYIADRLEKNFRRTVFAAVPQ
                     NPAQAQAQARPVGGFGRRVAGS"
     gene            5536..5994
                     /locus_tag="VN627_04075"
     CDS             5536..5994
                     /locus_tag="VN627_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02466.1"
                     /translation="MGRSVDENRFASLKPALLARKGGAKPAMRPQFAALEAAYGPIPD
                     DLGPEDLGWNDFGPEADAPDRIVPITRARPGRPERAAPAAAGARKAAFTLRLDPERHL
                     KLRLACALDARSAQALVTDALDRLLEAMPDLDLLTHDAAALRGKRRPAKA"
     gene            6016..7419
                     /locus_tag="VN627_04080"
     CDS             6016..7419
                     /locus_tag="VN627_04080"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXD02467.1"
                     /translation="MASTDFHLVLHREGGNRILRDRQSGVRLPLAVGSALAFSLLAGC
                     TGQGAIHSASAASADRAFAAEAAKSERAVAKAEAVVARTPQEAAARADLGRAYLAAGR
                     FESARTALNDAMTLGDQTGRTALSLALAQIGAGQFREAVALLDERRGDIPAGDLGLAL
                     ALAGESSRGVAILADAVRGGENTPKLRQNLAYAYALDGRWAEAKVMAAQDVPADQLDR
                     RLAIWALSALPDRSRDRVAGLLGAPVRMDPGQPAALALQTEAAAPRMADATPVVAPEP
                     ATELPAAELPAVAAAPQTTVATNLAAQSAPVAAAEPAVAVHRTPTLASVAQAFGAREL
                     KASKFRPTEPAVPARTRAIAAAMARPVAGQGTHVVQLGAFASEKNARRAWGIYTRENP
                     KLAAYRPVIVPAVIKGRQLWRVAAGGFTGRFAANGLCSQLRSHGSACFAYALPTRATP
                     APAAPGHDLGAPQRARR"
     gene            7634..8020
                     /locus_tag="VN627_04085"
     CDS             7634..8020
                     /locus_tag="VN627_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXD02468.1"
                     /translation="MHKKTMAVALAAAALATATGAQAQGWGVWVGSGQQYGYGDGGGG
                     WAVRAVCSGERARSLEGRLNHEEREGEIDPGTADRIHEAIDRLEDRSRNECAEGDRRA
                     IWNIAQRFDRIQGWMENEAHGDGRRW"
     gene            complement(8069..9301)
                     /locus_tag="VN627_04090"
     CDS             complement(8069..9301)
                     /locus_tag="VN627_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXD02469.1"
                     /translation="MISAPLTIVNARLVLPGCKPVAGALRSEGDRIVAVGPDVAAQSG
                     DRVHDAKGLLVTPGLVDLGVFAVDKPAFHFGGITRAALMPDQGPPLDHPARVRFAAQS
                     GKPGLWVHPLAAATRGLEGSEIAEIALMRESGARAVSTGRGWIADSGTMLRLLRYCAM
                     LGMVVVSHAEDSGLAGSAVATAGEMATRLGLPSAPAEAEALAVARDIALAEIAGARLH
                     LRQVTTAAGLDLVRAAKRRGVAVTAGVTPAHFMLSDTALGDFRTFLRLSPPLRSEADR
                     KAVITAIGDGTIDVIASGHDPRGPEDKRLPFADAEPGMAGAETLLATSLSLVRDGVID
                     VTRLIELLAETPARLLGVKAGRLEAGYEADVAVIDAERPWIVNSDKMAASAGNTPFDR
                     QGVQGRCVALFKGAAQVS"
     gene            complement(9298..10320)
                     /locus_tag="VN627_04095"
     CDS             complement(9298..10320)
                     /locus_tag="VN627_04095"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HXD02470.1"
                     /translation="MTAVQTPSGTSSGRYPAGSLAFPHRNLLGLAGLAPHELLFLLDE
                     AEQWVELNRQPKKRADLLRGLTIINAFFENSTRTLLSFEIAGKRLGADVVNMHAATSS
                     VKKGETLIDTALTLNAMRADAIVIRHASSGAVRLIADKVDCPVLNAGDGQHEHPTQGL
                     LDALTIRRALRARGETEETFSGLKVVICGDILHSRVARSNIHALTTLGAEVRVCAPPA
                     LMPAEIEQLKVTAYSDFDAALAGADVVMMLRLQLERMQGQFVPSPREYRHLYGLTLDR
                     LARAAPGALVMHPGPMNRGIEIDSNVADHAERSLITTQVEMGVAMRMTCLDVLTRRAR
                     GLEGWA"
     gene            10601..12472
                     /gene="sppA"
                     /locus_tag="VN627_04100"
     CDS             10601..12472
                     /gene="sppA"
                     /locus_tag="VN627_04100"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HXD02471.1"
                     /translation="MIFARKVWHLLVAIKDGLVLLFMLLFFGLLYGVLAMRPGPGAVR
                     DGALLLRLKGPVVEEPAAPQPLAVLSSGSLPAPQYRARDLVRALDAAAADGRIKAVVL
                     DLSEFTGGGFVHMAELGAAMDRVRAAKKPVLTYSLGYDDDATLLAAHSTEAWINPMGA
                     LYVLGPGGQQQYFKALLDKLKVTIHVYKVGTFKDAIEPWDRDSMSDASRQARTAVIQA
                     VFAQWQANVRAARPRANLDLVLKDPVGWMKAAQGDPARAALASGLVDRIGDRIEFGQR
                     VAQLAGKDQGDDRPGAFAHSSGAALLAAHPQSTAGKKIGVLTIAGDIVDGSAGPGAAG
                     GDRIAALIDGKDAKDLAGLVVRVDSPGGSVMASEQIRKAITRAKARGVPVAVSMANYA
                     ASGGYWVSTPANRIFAEPGTVTGSIGIFAVLPSFEKSLAAIGVKSDGVRTTPLSGQPD
                     VLGGFTPEISAMLQSGIEAGYARFVGLVAKSRGKSFADVDRIAQGRVWDGGTARQLGL
                     VDQFGTLDDALGWVAGQARLGQGEWHPVYLGQNANPYASLLEKLSGPDDSEAPEADVF
                     ARLAQRQQARLFAALAMADHLMGAHGAQALCLACPAMPTPQPAPKLGWLQALVELAK"
     gene            complement(12610..12789)
                     /locus_tag="VN627_04105"
     CDS             complement(12610..12789)
                     /locus_tag="VN627_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02472.1"
                     /translation="MATQPPPRPDQADPIDPSIPGELPPVITPDELPNEPQGVPEVAP
                     DFDQPDTGPSEVPEY"
     gene            12931..13015
                     /locus_tag="VN627_04110"
     tRNA            12931..13015
                     /locus_tag="VN627_04110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12965..12967,aa:Leu,seq:tag)
     gene            13077..14669
                     /gene="tig"
                     /locus_tag="VN627_04115"
     CDS             13077..14669
                     /gene="tig"
                     /locus_tag="VN627_04115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXD02473.1"
                     /translation="MQIVETTNEGLKRGYTVTIAATLIAERIEGEVAKIAPQVRMPGF
                     RPGKVPANLVKKMHGPALHNDALQAAIREAMDKLVADNALRPAMQPAVTLGEGYDLGQ
                     DAVLTVTLEVLPAIAAPSLEGLKLEKLVVPVSDEQVTEAITRIASGAKSFVDAKKGKK
                     AENGDQVTIDFVGKVGDEAFEGGSAEDVALELGAGRFIPGFEEQLVGVKEGDEKQLKV
                     TFPADYPAENLKGKDATFDVKVKAVKIAGETKLDDDFAKNLGLESLEQLQGLLRGQLE
                     QETAGLTRTQMKRQLLDQLAAGHDFAVPPSMVQAEFDQIWQQLLQEAQNEADPEAAKA
                     ELEAEQDDYRRIAERRVRLGLLLSEIGQANGVEVSQQEMSMLLQQAAQQYRPEDRQRF
                     IDYIRSEPLAAAQLRAPLYEDKVVDFLFDKAEVTERQVTRDELQAAIEAEEGAGSGHV
                     HGPDCNHDHGDKPAAKKKAPAKKAKADEAPAEAAAEEAPAEKAPVKKAPAKKAAAAKA
                     DEAPAAEVAPKKKAPAKKPAAK"
     gene            14855..>15130
                     /locus_tag="VN627_04120"
     CDS             14855..>15130
                     /locus_tag="VN627_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02474.1"
                     /translation="MLPFMCTPLPPLGDLYSHIGRYHVMLTHGESPYLDRYYEFHWAL
                     IPNMGQDLLMVPVGRLLGAERGGILLSALILPATILGIRAVSIAAHGR"
BASE COUNT         2368 a         5553 c         4985 g         2224 t
ORIGIN      
        1 tagggcttga aggtcatgac ggcgccgact ttgggcgttg ttccgcgcgc gtagcggcca
       61 tcggcctggt cccaccaggt ccgcgcgtcg ccaaagatca gcacgccgga aacggcccgc
      121 gcataaggca cgcactggag atagggtggc aggttcgcgc tgccgccgcc cagaacgtgg
      181 tcgctgtcga ccaccgtgtc ggctcgcgcc ggcgcggcgg cggcgcccag cgccatggcg
      241 atcaggattg cggttttgcc ccacatacgg caactcactg gccagtttcc ggttaatcca
      301 gtctcgccaa acagggttaa caattggttg gcgccgtgtt cgtggccgct gccatagtgc
      361 ccccgcgctt gcgccggagg cggaaaaccg gcactggccc gggatcatgc tcccccaggt
      421 aatcatcatc gggcgtccca acgtcggcaa gtcgaccctg ttcaaccgcc tggtcggcaa
      481 gaagctcgcc ctggtggatg accagcccgg cgtcacccgc gatcgccgct ttggcgaggc
      541 gcacctgctc ggcctcgatt tcaccatcgt cgatacggcc ggctgggagg acgaggatgc
      601 cgccagcctg ccgggccgca tgcgccagca gaccgaggct gcgctgaagg gcgccgatgt
      661 cgccctcttc gtggtcgacg cccgcgccgg gatcaccccg ctggacgagg aaatcgcccg
      721 ccacctgcgc tccagcacgg tgccgatcgt gctgctggcc aacaaggccg aaggccgggc
      781 cggcgatccc gggctctatg aagccttctc gctcggcttc ggcgaaccca tcgccttttc
      841 ggccgagcac gggcagggac tggccgatct gttcgaggcc ctgctgcccc atgtggacga
      901 caagcagcgc gaacacgagg agcaggtcgc cgccgacgag gccgaggacg attatgatcc
      961 ccaggccatt ctcaagctcg ccatcgtcgg ccggccgaat gccggcaagt ccaccctgat
     1021 caatgccctg cttcgggaag accggctgct gaccgggccg gaagccggga tcacccgcga
     1081 ttccatcgcg gtggactgga cctggaccga tccgaaaacg aaggccgagc gccccgtgcg
     1141 cctgatcgac acggccggca tgcgcaagaa ggcgcaggtc gtcgagaagc tggaaaagct
     1201 gtcggtcgcc gatgcgcgcc atgcgatcga ctttgccgag gttgtcgtcc tcctgctcga
     1261 tgccacccgc gggctcgagc accaggatct caagatcgcc tcgatggtgc tggaagaggg
     1321 ccgggccctg atcatcgcga tcaacaagtg ggacgtggcc gaggatgcct cgggcctgtt
     1381 caacggcgtg cgcgccgcgc tcgatgaagg ccttgcccag gtgcgcggcg tgcccctgct
     1441 gtcggtctcc gcgcgcaccg gcaaggggct cgataccctg atccaggccg ccttcgatat
     1501 ccgcgatgcc tggtcgaagc gcgtgcccac ggctgcgctc aaccgctggt tcgacgatgc
     1561 gctggctgca aacccgccgc ccgcgccggg cggacggcgg atcaagctgc gctacatcac
     1621 ccaggcgaaa acccgcccgc ccggcttcgt cctgttcggc acccggctcg atcagcttcc
     1681 cgaatcctac cggcgctatc tcgtcaacgg catccgccgc gaactgggtt tcgatgcggt
     1741 gccgatccgg ctgaccctcc gcagcccgaa gaaccctttc gccaaaaagt agcgccgatt
     1801 tcgccctaga gccccagccg tgcccagaac tcgctggcgc tggcgaggcg ctggtcttcg
     1861 cctccgggcg cgctgcccag ggccagccgc cacgaggggt ggggcaggcg caggctgtcg
     1921 ggctgaggcc ggcggcgcag caggcggacg ggagcggaac ggatagaatt ggccatttcg
     1981 tcttctccat gagggaacac gaaatcacgc gcggtcgcga aggcttcgtg cgctttagcc
     2041 gttggtgacg cggagcaagc tgacaggatc aaaagccgcg atcgcatccg gaaaccggca
     2101 cgataggtaa actctaccaa ttcgctggag attgtcgaga taggttctct tgcgacgccg
     2161 caacgcgcac ttgccgccgc aagggctgcc accggcccca ggtggccgag cgccacagcc
     2221 actcaagcgg accgtggcga aaccgggcga gccacggctg gctccacccc agcatcagcg
     2281 cccagccggc cagcaccagc agccattgcc agcgttccgg catggtgccg atcaggccca
     2341 gcccccagcc ctggaacaga gcgcacatca cggcagtcat ggcgatgtag ttggtgaagg
     2401 ccatccgccc cgccgcgctc agccgctgcc ccagccatgt cggcgcaaac cggggcacgg
     2461 ccagcagcag cgcggcgccg tagccgagcg ccatggccag gtgtggcagc gccaggccac
     2521 aggcgagggc ggcctgcatc aggtccggcg ggaattgacg cggccaggcc cagccgagga
     2581 aggcaagcgt cgctgcgccg ccgcccgcga tgccgcccac cgccaggcgc cgcagggtgc
     2641 gccgccgcac ctgccccgaa tagaatccgc cgcgatagag cgccatgccc agcagcatca
     2701 gcggcagggt ctcgcccatc ccggccagcg ccgagctcac cggctcgctc ggctcttcgc
     2761 gccacttggc ggcaacctgc gcggcaaaac ccatccggtc gcgcgccact tcgcgctgcg
     2821 tgtctgcgcg aacctcgtcg cgcaccaggg cataggccac cgcctcgccc ggcgaggcat
     2881 ggccggacag cacccgctcc tcgcgcgcaa cgtcggggcc ggaggcaacc gccagcagca
     2941 ggtgccaccc ggcaaacagc gccagcgccc ccgcgcccag cgcgcgcggg ggcgcctggc
     3001 ggaaggcgag ggcggcaaac ccggccagcg cataggtgaa caggatgtcg ccccaccaca
     3061 gcagcaggaa gtgcaggtaa cccgccagcg ccagccagcc gaggcggcga aactgcagcc
     3121 gctcgccgtt cagcccgcgc cggtcggccc gctccatgaa caggaccagc gaggcgccga
     3181 acagcagggt gaacagcgcc cgcatcttgc cctcgaacac cacgagggcg aaggcccagg
     3241 ccgcttcgtc cgcgaatgtc cccggctcca gctggtttgg cgaaagcgtc gccgcgatcg
     3301 gcccggcaaa gccgccgatg ttgatggcga gaataccgag cacggcgacc ccgcgcaaca
     3361 gatccaggct ggcgaggcgg cccttctcgc cagcgtgagg cagcagcccg ggggccgcgt
     3421 ccaagtgatc agtcctgcgt cacgagacag gtgaagcccg cccgggtcag ccccgcacag
     3481 gccgaagctg ccgcgccggg cgaggcaaag ccgcccgcct gcagcttgct gaccttgccg
     3541 gcgcgcaggt cgatccggcg gtgcccggcc agctccggcc gggccgatgc cctggcccac
     3601 agcgcgtcgg cattggcggc caccccgaag gcgccgagct gcacccgcca gatgccgccg
     3661 gtcacggcag cggcgggcct tggtttcggc gcgagcgcca ccgccaccgc aggcttggca
     3721 ggcgccggct tgagcgcggg cgctgcggcc cccaccggct tgggcgccgc gcccaccgcc
     3781 ggccgcaccg caacgggctt ggctccgggc acgggcaggg gcggggcctg cgccccggcg
     3841 ggccgggcat agtccgcccc ggccgttgcg gcgccgcccg gttccggcac acccaccggc
     3901 ggcggcgcat ccgcagccag cggctttgcc gccgccggtg cctcgccaag atcgttgctg
     3961 gcgatctggg tattgcgggt ggtctccgcc tgctgcgcca gctgcggtgc aaggctggcg
     4021 ccctgctggc gctcggccag cgggatgaag ctgtccatct gctggatcgc cggggcggcc
     4081 tgcggcagcc cggccgagcg ggccagcgtc atcagcgcat aggcgcgcac ccagtccttg
     4141 gccgcaaggt ccccgttgaa atgggcaatc gccatcagat actgcgcgcg cggatcgccc
     4201 cggtccgacg ccgccttgac atagggcatg gcctgggccc gggccccgcg atcgaacatc
     4261 agcaggccat agttgtccgc cgcctgcaca tggccctgtg cggcggcctt gccgaacagc
     4321 tcctcggccc ggcccagatc gcgcggcacg ccccggccga gccggtaggc ctggcccagg
     4381 ttgaactcgg catcgggatc gccggacctg gccggctcct gccactgggc cacggccccg
     4441 gcatagtcac cccggctcca ggcatcggcc cccgccttga cgtcggcctg agcgggcagg
     4501 gccgccatgg ccaccaatgc agcggccggc cagatccgga ttcccctcat ggctccccct
     4561 caagcgtcat gcgtccgcct gccccgcgac ggtttcaatc ggtatgcttg tgccacacgg
     4621 gccacgcgga acaagccccc acgcccggat ttcccgccat ttcggtgcct ccccccgatc
     4681 ccttcgcggg cgccgcgtta acctgatctt agggacccca tgctttggtt cgcgtgaggt
     4741 gatgcgtttg ctggggggat attctcccgt tcggcggatc gccagcaccg cattcaagtc
     4801 gggggtatga actttgcggg ttttggcaat tgcatcgcag aagggcggct cgggcaagac
     4861 cacgctctcc gggcatctgg cggtccaggc gcagcgcgcc ggggctggtc cggtcgtgct
     4921 gatcgatatc gatccgcagg gctcgctggc cgattggtgg aacgaacgcg cggacgattt
     4981 ccccgccttc gcccagacca ccgtcgcccg cctcgcggcc gatctccagg tgttgcgcca
     5041 gcagggcttc aagctcgcgg tcatcgatac ccccccggcg atcaccatgg cgatccagtc
     5101 ggtcatcggc gtggccgaac tgatcgttgt tccgacccgg ccgagcccgc acgacctgcg
     5161 ggccgtcggc gcaacggtcg atctgtgcga acgcgcgggc aagccgctgc tgttcgtggt
     5221 caacggcgcc acccccaagg cgcggatcac cagcgaggcc gccgtcgcgc tctcgcagca
     5281 tggcaccgtc gccccggtca ccctgcatca ccggaccgat ttcgccgcct cgatgatcga
     5341 tgggcgcacg gtcatggaag tcgatcccaa cggccgctcg tccatggaag tcgccgcgct
     5401 gtggggctac atcgcggacc ggctggaaaa gaacttccgc cgcaccgtct tcgccgcagt
     5461 gccgcagaac ccggcccagg cccaggccca ggctcgcccg gtcggcggct tcggccgccg
     5521 cgtggcaggc tcgtgatggg ccgctcggtg gatgagaacc gcttcgcctc gctgaagccg
     5581 gctctgctcg cccgcaaggg cggggccaag cccgccatgc gcccgcaatt cgccgccctt
     5641 gaggcggcct atgggcccat tccggacgat ctaggccccg aggatctagg ctggaacgat
     5701 ttcggtcccg aggccgatgc ccccgatcgc atcgtcccga tcacccgtgc ccgccccgga
     5761 cgcccggaac gcgccgctcc cgcggccgca ggcgcgcgca aggcggcctt caccctgcgg
     5821 ctcgatcccg agcggcacct caagctgcgc ctcgcctgcg cgctcgatgc ccgcagcgcc
     5881 caggcgctgg tgaccgacgc gctcgaccgc ctgctcgagg ccatgcccga cctcgatctc
     5941 ctgacccacg acgctgccgc cctgcgcggc aagcgtcgcc cggccaaagc ctgatttttg
     6001 cgaggagtta tttctatggc ctcgaccgat ttccacctcg tcctgcaccg cgaaggcggc
     6061 aaccgcatcc tgcgcgatcg ccagtccggc gtgcgcctgc cgctggccgt cggctcggcc
     6121 ctggcctttt cgctgctcgc gggctgcacc gggcagggtg cgatccactc cgccagcgcc
     6181 gcctcggcgg accgggcttt cgccgccgaa gcagcaaagt cggaacgcgc cgtcgccaag
     6241 gccgaagccg tggttgcccg caccccgcag gaggctgccg cccgtgccga tctcggccgg
     6301 gcctatcttg ccgccggacg cttcgaatcc gcccgcaccg cgctcaacga cgcgatgacg
     6361 ctcggcgacc agaccggccg caccgccctc tcgctcgccc ttgcccagat cggcgcgggc
     6421 cagttccgcg aagcggtcgc cctgctcgat gaacgccgcg gcgacattcc cgccggcgat
     6481 ctcggccttg cccttgccct tgccggcgaa agctcgcgcg gggtcgcgat cctcgccgat
     6541 gccgtgcgcg gcggggaaaa cacccccaag ctgcgccaga acctggccta tgcctatgcg
     6601 ctcgacgggc gctgggccga ggccaaggtc atggccgcgc aggacgttcc ggccgaccag
     6661 ctcgaccgcc gcctggcgat ctgggccctt tccgcgctgc ccgaccgcag ccgtgaccgt
     6721 gttgccggcc tgctcggcgc gccggttcgg atggacccgg gccagcccgc cgccctggcc
     6781 ctgcaaaccg aagccgccgc tccccggatg gccgatgcca cgccggtcgt cgcgcccgag
     6841 ccggccaccg aactgccggc cgccgaactg ccggcggttg ccgccgcgcc gcagaccacc
     6901 gtcgccacga acctcgcggc ccaatcggct ccggttgcgg ctgccgagcc cgccgttgcc
     6961 gttcaccgta cgccgaccct cgccagcgtg gcccaggctt ttggcgcgcg tgaattgaag
     7021 gcaagcaagt tccgcccgac cgagccggca gtgcccgccc gcacccgcgc cattgccgcc
     7081 gccatggccc gcccggtcgc tggacagggc acccatgtcg tccagctcgg tgccttcgcc
     7141 agcgaaaaga acgcccgccg cgcctggggg atctacaccc gcgagaaccc gaaacttgcg
     7201 gcctatcgcc cggtgatcgt ccccgccgta atcaagggcc ggcagctgtg gcgcgtcgcc
     7261 gccggcggct ttaccggccg cttcgccgcc aatggcctgt gcagccagct gcgctcgcat
     7321 ggatcggcct gcttcgccta tgccctgccg acccgcgcca ccccggcgcc ggccgcaccg
     7381 ggccacgatc tcggcgcccc gcagcgcgcg cgccgttaac ctttttttga ctatgccggc
     7441 gcatcatcgc gccagcatga aacagcctct ccctgacccc gccgggcaac cggcggggtt
     7501 ttttctcgtg cgctcaagtg aaggcaatcg gcgccaagct gaacgccggc caaccgaatc
     7561 gttcagcctc gctccaaggc caccggcata gcctgcaacc tcctgaacat ccgggaagcg
     7621 atggaggttt ggcatgcaca agaagacgat ggccgtggca ctcgccgcag cagccctggc
     7681 aacggcaacc ggcgcccagg cgcaaggctg gggcgtctgg gtcggctccg gccagcaata
     7741 cggctacggc gacggtggcg gcggatgggc cgtgcgggcg gtctgctcgg gcgagcgggc
     7801 gcgcagcctc gaaggccggc tgaatcatga agagcgcgag ggcgagatcg atccaggcac
     7861 ggccgaccgg atccacgagg ccatcgaccg gctggaagac cgttcgcgca acgaatgcgc
     7921 cgaaggcgat cgccgcgcca tctggaacat cgcccagcgc ttcgaccgca tccagggctg
     7981 gatggagaac gaagcccacg gcgacgggcg ccgctggtaa gcctgcctga aaccggtcgc
     8041 ccggcctttt ggtcgcgcgg ccggtgcctc aggaaacctg tgccgcgccc ttgaacagcg
     8101 ccacgcagcg gccctgcacg ccctgccggt cgaacggcgt attgcccgcg ctcgccgcca
     8161 tcttgtccga attgacgatc cacggccgct ccgcgtcgat caccgcgacg tcggcctcgt
     8221 agcccgcctc gagccgaccg gccttcaccc ccagcagccg cgccggggtt tctgccagca
     8281 gctcgatcag ccgcgtcacg tcgatcacgc cgtcgcgcac cagcgagagg ctggtggcga
     8341 gcagcgtttc cgccccggcc atgcccggtt cggcatcggc aaagggcagg cgcttgtctt
     8401 ccggcccgcg cggatcgtgg cccgaggcga tgacgtcgat ggtcccatcg ccgatcgccg
     8461 tgatcaccgc cttgcggtcg gcctcgctgc gcagcggcgg ggaaaggcgc aggaacgtgc
     8521 ggaaatcgcc cagcgccgtg tccgaaagca tgaagtgcgc cggcgtcacc cccgcggtca
     8581 ccgccacgcc gcgccgcttg gccgcgcgca ccaggtccag tccggcggcg gtcgtcacct
     8641 gccgcaggtg cagccgcgcc ccggcgattt cggccagagc aatgtcgcgc gccacggcca
     8701 gcgcctcggc ctcggccggg gcgctcggca ggccgaggcg cgtcgccatc tcgcccgctg
     8761 tcgccacggc gctaccggcg aggccagagt cttcggcatg gctgaccacc accatgccca
     8821 gcatcgcgca gtagcgcagc agccgcagca tcgtgcccga atcggcgatc cacccgcgcc
     8881 ccgtgctcac ggcgcgcgcg cccgactcgc gcatcagcgc gatctcggcg atctcgctgc
     8941 cttccagccc gcgcgtcgcc gccgccagcg gatggaccca cagccccggc ttgcccgact
     9001 gcgcggcaaa ccgcacccgc gccggatggt cgagcggcgg cccctggtcg ggcatcagcg
     9061 ccgccctggt gatcccgccg aaatgaaacg ccggcttgtc gaccgcgaac acgccgagat
     9121 cgaccaggcc cggagtgacc agcaggccct tggcatcatg aacccggtcg cccgactgcg
     9181 cggcaacatc cggccccacg gcgacgatcc ggtccccttc gctgcgcagc gcgcccgcaa
     9241 cgggtttgca ccccggcagc accagccgtg cattgacgat ggtcagcggc gcggaaatca
     9301 tgcccagccc tccagcccgc gcgcccgccg ggtcagcaca tcaaggcagg tcatccgcat
     9361 ggccacgccc atttccacct gggtggtgat cagcgagcgt tcggcatggt cggccacgtt
     9421 gctgtcgatc tcgatgcccc ggttcatcgg gccgggatgc atcaccagcg cccccggcgc
     9481 ggccctggcg aggcggtcca gcgtcaggcc gtagagatgc cggtattcgc gcggcgaggg
     9541 cacgaactgg ccctgcatcc gctccagctg gaggcgcagc atcatcacca cgtcggcccc
     9601 ggcgagcgcg gcatcgaaat cggaataggc ggtcaccttc agctgctcga tctccgccgg
     9661 catcagcgcc ggcggggcgc agacgcgcac ttccgcgccc agcgtggtca gcgcatgaat
     9721 gttggagcgg gcgacgcggc tgtgcagaat atccccgcag atgacgacct tcagcccgct
     9781 gaaggtttct tccgtctcgc cccgcgcccg cagcgcccgg cggatcgtca gcgcgtcgag
     9841 caggccctgc gtcgggtgtt catgctgccc gtcgccggca ttgagcaccg ggcagtccac
     9901 cttgtcggca atcagccgca ccgcgccgga actcgcatgc cggatcacga tcgcgtcggc
     9961 ccgcatcgcg ttgagcgtca gcgccgtgtc gatcagcgtt tcgcccttct tcacgctgga
    10021 tgtcgccgca tgcatgttga ccacgtcggc cccgagccgt ttgccggcga tctcgaagct
    10081 cagcagcgtg cgggtggaat tctcgaagaa ggcgttgatg atggtgagcc cgcgcagcag
    10141 gtccgcccgc ttcttgggct ggcggttcag ctcgacccat tgctcggcct cgtcgagcag
    10201 gaacagcaat tcgtggggcg ccaggccggc gaggcccagc aggttgcgat gcgggaaggc
    10261 caggctcccg gccggatagc ggcccgagct tgtccccgac ggggtttgca cagctgtcat
    10321 tgaagaccat gccttaggga agggcggcgc gcggctcaag ggggcgcgcg cgcgatgtcc
    10381 acgattgtct ttgctgaggc tcgctttgct cgcgacaaag accgcatggc ctttgcgcgg
    10441 caccagccca ctcccccggc ccgaccaccc atcaagtatg cttccgtagg tggtcgggcc
    10501 gggggagtgg gctggtgccg caagccttcg acggatgtcg aaggcgcccc cttcaaacgt
    10561 cgccttcccc cgatccccca aaccccctat caccccggca atgatattcg cgcgcaaggt
    10621 ctggcacctc ctggtggcca tcaaggacgg cctcgtcctc ctgttcatgc tgctgttctt
    10681 cggcctgctc tacggcgttc tggcgatgcg gcccggcccc ggcgcggtgc gcgatggcgc
    10741 gctgctgctg cggctcaagg gccccgtcgt ggaagaaccc gccgcgccgc agccgctggc
    10801 cgtgctatcc tccggcagcc tgcccgcccc gcaataccgc gcccgcgatc tcgtccgcgc
    10861 gctcgatgcg gcggcggcgg acggccggat caaggccgtg gtgctggacc tctccgaatt
    10921 caccggcggc ggcttcgtcc acatggccga acttggcgcg gcgatggacc gggtgcgcgc
    10981 ggcgaagaag ccggtgctga cctattccct cggctacgac gacgatgcca ccctgctcgc
    11041 cgctcattcc accgaggcgt ggatcaaccc gatgggcgcg ctctacgtgc tcggcccggg
    11101 cggccagcag cagtatttca aggccctgct cgacaagctc aaggtcacca tccacgtcta
    11161 caaggtcggc accttcaagg acgcgatcga gccgtgggac cgcgattcga tgtccgatgc
    11221 ctcgcgccag gcccgcaccg ccgtgatcca ggccgtcttc gcccagtggc aggcgaatgt
    11281 ccgcgccgcc cggccgaggg cgaacctcga tctcgtcctc aaggacccgg tcggctggat
    11341 gaaggccgcc cagggcgatc cggcccgcgc cgcgctcgcc tcggggctgg ttgaccgcat
    11401 cggtgaccgg atcgagttcg gccagcgcgt tgcccagctc gccggcaagg atcagggcga
    11461 cgatcgcccc ggcgcctttg ctcactccag cggcgcggcc ctgcttgccg ctcaccccca
    11521 gtccaccgcc gggaagaaga tcggcgtgct gaccattgcc ggcgatatcg tcgatggctc
    11581 cgccgggccc ggcgctgccg ggggagaccg cattgccgcc ctgatcgacg gcaaggatgc
    11641 caaggacctc gccggcctcg tcgtccgggt cgattcgccg ggcggttcgg tcatggcctc
    11701 cgaacagatt cgcaaggcga tcacccgggc caaggcgcgg ggcgttccgg tggcggtctc
    11761 catggccaac tatgcggcca gcggcggtta ctgggtctcc acgcctgcca accgcatctt
    11821 cgcggaaccc ggcacggtta ccggttcgat cggcatcttc gccgtgctcc cctcgttcga
    11881 gaaatcgctc gccgccatcg gggtcaagtc cgacggggtg cgcaccacgc cgctgtccgg
    11941 acagcccgat gtgctcggcg gcttcacccc cgagataagc gccatgctgc aatcggggat
    12001 cgaggccggc tacgcccgct tcgtcggcct ggttgccaag tcacgtggca agagcttcgc
    12061 cgacgtcgac cggatcgcgc agggccgtgt ctgggacggc ggaaccgccc gccagcttgg
    12121 cctcgtcgat cagttcggca cgctcgacga tgcgctcggc tgggtcgccg ggcaggccag
    12181 gctggggcag ggggaatggc acccggtcta cctcggccag aatgccaatc cctatgcttc
    12241 cctgctggaa aagctctccg gcccggatga cagcgaggcc cccgaagccg atgtcttcgc
    12301 ccgccttgcc cagcgccagc aggcccgcct gttcgccgcc ctcgccatgg cggaccacct
    12361 gatgggcgcg cacggcgccc aggcgctgtg cctggcctgc ccggccatgc cgacgccgca
    12421 acccgcgccg aagctgggct ggttgcaggc gctggtggaa ctggcgaaat agcgtcgtcc
    12481 cgggcttgac ccgggaccgc tggccaatgc gaagctggaa cggcagcatt caagcgcatc
    12541 agcgctgaca ggcaagccca cagctccgca ggcgccaccg gtcccgggtc aagcccggga
    12601 cgacgcctgt caatactccg gcacctcgct cggcccggtg tccggctggt cgaagtccgg
    12661 cgccacctcg ggcacgccct gcggctcgtt cggcagctcg tccggcgtga tcaccggcgg
    12721 cagttcgccc ggaatggagg gatcgatcgg gtccgcctgg tcgggccggg gcgggggctg
    12781 ggtagccatg gtcttctcct gaagtcgcgg aaacgccgga cgactccagc gcgttggccc
    12841 caatatagtt cctcccatcc gccagcgctt gcccttttcg cgcgaggtcg ctacgggcca
    12901 cggctcttgg atttcgggct tcgtcgacat gcgggcgtgg cggaactggt agacgcagca
    12961 ggtttaggtc ctgccatcgc aagatgtggg ggttcgagtc ccttcgcccg caccagtccg
    13021 gacaccctcc cgcaccgacg aagccgcaac gcgttgttga gaaggcattt atcatcatgc
    13081 agattgtcga gaccacgaac gagggtttga agcgcggcta tacggttacc atcgccgcca
    13141 ccctgattgc cgagcggatc gagggcgagg tcgccaagat cgccccgcag gtgcgcatgc
    13201 ccggcttccg ccccggcaag gtcccggcca atctggtcaa gaagatgcac ggcccggcgc
    13261 tccacaacga cgccctccag gccgcgatcc gcgaggcgat ggacaagctg gtggccgaca
    13321 atgcgctgcg cccggcgatg cagccggccg tcaccctggg tgagggctat gacctcggcc
    13381 aggacgcggt gctgaccgtc accctcgaag tcctccccgc catcgccgcg ccctcgctcg
    13441 aaggcctcaa gctcgaaaag ctggtcgtcc ccgtcagcga cgagcaagtc accgaagcga
    13501 tcacccgcat cgcctcgggc gccaagagct ttgtcgatgc caagaagggc aagaaggccg
    13561 agaacggcga tcaggtcacc atcgatttcg tcggcaaggt cggcgatgag gcgttcgaag
    13621 gcggcagcgc cgaggacgtc gcgctcgaac tgggcgccgg ccgcttcatc cccggcttcg
    13681 aggaacagct cgtcggcgtg aaggaaggcg acgagaagca gctcaaggtg accttcccgg
    13741 ccgactatcc ggccgagaac ctcaagggca aggacgccac gttcgacgtc aaggtgaagg
    13801 ccgtgaagat cgccggcgag accaagctcg acgacgattt tgccaagaac ctcggcctcg
    13861 aaagcctcga gcagctgcag ggcctgctgc gcggccagct tgagcaggaa accgccgggc
    13921 tcacccgcac ccagatgaag cgtcagctgc tcgaccagct cgccgccggc cacgatttcg
    13981 ctgtgccgcc ctcgatggtc caggccgagt tcgaccagat ctggcagcag ctgctccagg
    14041 aagcccagaa cgaagctgac ccggaagccg ccaaggcgga actggaagcc gagcaggacg
    14101 actatcgccg catcgccgag cgccgcgtgc gcctcggctt gctgctctcg gaaatcggcc
    14161 aggccaatgg cgtggaagtg agccagcagg aaatgtccat gctgctccag caggccgccc
    14221 agcagtatcg cccggaagac cgccagcgtt tcatcgacta catccgctcc gagccgctcg
    14281 ccgccgccca gctgcgcgcg ccgctctatg aggacaaggt cgtcgacttc ctgttcgaca
    14341 aggccgaggt gaccgagcgc caggtgaccc gcgacgaact ccaggccgcg atcgaggccg
    14401 aagagggcgc cgggtcaggc cacgtccacg gtccggactg caaccacgac cacggcgaca
    14461 agccggcggc caagaaaaag gctccggcca agaaggcgaa agcggacgag gctccggccg
    14521 aagccgctgc cgaggaagcc cccgccgaaa aggctcccgt taagaaggct ccggccaaga
    14581 aggctgctgc cgccaaggct gacgaggctc ccgctgcgga ggtcgcgccc aagaagaagg
    14641 ctccggccaa gaagcctgct gccaagtagg ttgcgtcaac ccctgggttt tgcttctact
    14701 cccccggcaa gctctcgata ggggtgccgg ggggaaaatg gacgtatttg cgccgctaac
    14761 gcggtttgtt gtcttgccgg gccaggcggt atgcgccgac cgtctgcgct ggtacgaatt
    14821 gcggctgacg tggcttgccc tggccttggt gtcagtgctg ccgtttatgt gcacgccgct
    14881 accgccgctg ggcgatctct acagccatat cggacgatac cacgtcatgc tgacgcatgg
    14941 ggaatcgccc tatcttgacc ggtattacga gttccactgg gccctgatcc ccaacatggg
    15001 gcaggacctg ttgatggtgc cggtgggccg gctgctcggc gcggagcggg gcggtattct
    15061 gctgtccgcc ctcatcctgc cggcgaccat cctgggcatc cgggccgtaa gcatcgcggc
    15121 gcacgggcgg
//
LOCUS       DATNBV010000170         4042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3930549,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000170 DATNBV010000000
VERSION     DATNBV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3930549"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VN627_04125"
     CDS             complement(<1..278)
                     /locus_tag="VN627_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXD02475.1"
                     /translation="MAHDLTIGFLPLVDACLPILAREHGFAEAEGLSLTMVRDMSWAT
                     VLDRLLYGHSDAAHLVAPLAIATTLGRGRPAAPLSVPFVLGLNGNAITL"
     gene            complement(278..856)
                     /locus_tag="VN627_04130"
     CDS             complement(278..856)
                     /locus_tag="VN627_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HXD02476.1"
                     /translation="MRIAIVDESATRAAVIGEGLAAFDDCELFVVTERRGLVARIAEI
                     DPDIVLMDLGNPSRDMLEEYFAVSRALARPIAMFVDESDDEAIAASIDAGVSSYVVDG
                     LAAHRIRPILDLAVRRFNAFSRLQADLAEAKGKLADRETVDKAKRILMDSRGLAEPQA
                     YAELRKAAMDQGKRIAEIAEAVVTAHRLMGGT"
     gene            complement(944..1018)
                     /locus_tag="VN627_04135"
     tRNA            complement(944..1018)
                     /locus_tag="VN627_04135"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(984..986),aa:Thr,seq:tgt)
     gene            complement(1094..1990)
                     /locus_tag="VN627_04140"
     CDS             complement(1094..1990)
                     /locus_tag="VN627_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXD02477.1"
                     /translation="MTIPAGDGPLLGAVEAGGTKFVLAIGTASGKVLASHRLPTTTPA
                     ETLAATADWFSAQAPLAAIGVGSFGPAVLDPASPRYGHITDTPKPGWSDCDIAGHFAR
                     RFGVPIGFDTDVNGAALGEYLHGAGKAGQSLAYVTVGTGIGGGLVIGGEPVHGASHPE
                     MGHLFPRRPAGDTVFAGTCPFHGDCLEGLASGPAIKARWGASLDHLPAGHEAHAIVAD
                     YLAQLCHTLFALTAVETIVLGGGVSQAPGLVERVRRRSAELSAGYLPGGKRHAVVAPG
                     LGQNAGIVGALVLAARAIAQSA"
     gene            complement(1987..3111)
                     /locus_tag="VN627_04145"
     CDS             complement(1987..3111)
                     /locus_tag="VN627_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 68 protein"
                     /protein_id="HXD02478.1"
                     /translation="MTTAWTPAQVRTIDPATAPRVPVIAGAERSDLLPGLVYWDMWPI
                     QDATGHIARLGERELWMALSAPHRGDPALRHFEAKIRLLERTGPLGAERWTDLGPVLP
                     DGPVPYEREWAGSASTDGTRVSLWFTGAGTSERPGGYQQALYEAHAIIGPDGLPRDWS
                     APQLSLASGPAYQSADAHDGEPGRIKAFRDPAWLCNPADGAEYLVFTASVAGASSPFN
                     GAVGLARRTADGWEPLPPLVTADGVNNELERAHVVVHQGRYLVFWVTQTSTFSPQLRS
                     APSGLYGMIADSLAGPWRPLNGSGLVLANPVDEPLQSYSWFVSADLVVSSFVDIWGLR
                     GGPCPVDTAALARHFGGVPAPLLQLVVEGDCCRIAERTTA"
     gene            complement(3211..>4042)
                     /locus_tag="VN627_04150"
     CDS             complement(3211..>4042)
                     /locus_tag="VN627_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXD02479.1"
                     /translation="VNGDGTIQPVERSVSLIDNANPSPVNYSVGYVSWSIGANYRVSP
                     DAAVFARASRGGRANADRLLFGVVRPDGSVRKDDAIDYVNQYELGFKYRSGPFGLFVT
                     GFHATTQEQNFEVTSGRFLDRTYKATGVEIEAAWRSGIFDLRGGATYTHARIAADAIT
                     PANKGHTPRRQAELIYQFTPSLDFDVARVGLNIIGTTKAYAQDENNLVFPGYTQVNAF
                     VNVRPMKGVELSLNANNLFNVVGITEAEEGSIVNNANNYVRARSINGRTVSASLRYDF
                     "
BASE COUNT          640 a         1247 c         1540 g          615 t
ORIGIN      
        1 agggtgatcg cattgccgtt gaggccgagc acgaagggga ccgacagcgg ggccgccggt
       61 cggccccgcc cgagcgtggt ggcgatcgcc agcggggcca cgagatgggc cgcgtcggaa
      121 tggccataga gcaggcggtc aagcacggtt gcccagctca tgtcgcgcac catggtcagc
      181 gacaggcctt cggcctcggc gaagccgtgc tcgcgggcaa ggatcggcag gcaggcatcg
      241 acgagcggga gaaagccgat cgtcaggtca tgggccatca ggttcctccc atcagccggt
      301 gcgcggtaac caccgcctcg gctatttcgg caatgcgctt gccctggtcc atcgcggcct
      361 tgcgcagttc ggcataggcc tgcggttcgg caaggccgcg gctgtccatc aggatgcgct
      421 tggccttgtc gaccgtctca cggtcggcca gcttgccctt ggcctcggcc aggtccgcct
      481 gcaggcgcga aaacgcgttg aatcggcgca ctgcaaggtc gaggatcggg cggattcggt
      541 gtgccgccag gccgtcgacc acgtaggacg acaccccggc atcgatcgag gcggcaatcg
      601 cctcgtcgtc gctttcatcg acgaacatgg caatcggacg ggccagggcc cggctcaccg
      661 cgaagtattc ctcgagcatg tcgcgcgagg gattcccgag gtccatcagc acgatatccg
      721 gatcgatctc ggcaatccgg gcgaccagcc cgcgccgctc ggtcaccacg aacaactcgc
      781 aatcgtcgaa cgcggccagc ccttcgccga tcactgcggc gcgggtggca ctttcatcga
      841 cgatggcaat ccgcattcct gtcattgtgc agtgcaatag ggctgagtgc aacacgcagg
      901 gccatgtcgc ctgtgcgacc cagccatcgg agggacagga taatggtgcc ggctgaggga
      961 tttgaacccc cgaccttcgg tttacaaaac cgctgcacta ccactgtgct aagccggcgc
     1021 tgcccaagct gggcgggtgt ggggacgcct ttgccctaag gcgcgaccaa ggtctaggct
     1081 gtatcgccgg cggtcaggcc gattgcgcga tggcgcgggc ggcgaggacg agggcgccga
     1141 cgatgccggc gttctgaccc aggcccgggg cgacgaccgc atggcgcttg ccgccgggga
     1201 gatagccggc cgatagctcc gcgctgcgac ggcgcacgcg ctcgacgagg ccgggggcct
     1261 ggctgacgcc gccgcccagg acgatggttt cgaccgcggt cagggcaaac agggtgtggc
     1321 acagctgggc gaggtaatcg gcgacgatgg cgtgggcctc gtggccggcg ggcagatggt
     1381 cgagcgaggc gccccagcgg gccttgatcg ccgggccgct ggcgaggcct tcaaggcaat
     1441 cgccgtggaa cgggcaggtg cccgcaaaga cggtgtctcc cgcgggacgg cgggggaaga
     1501 ggtggcccat ctctggatgg gaggcgccgt ggaccggctc gccgccaatc accagcccgc
     1561 cgccgatgcc ggttccgacc gtgacatagg cgagcgactg gccggccttg cccgcgccgt
     1621 gcaggtattc gcccagggcc gcgccgttga cgtcggtatc gaagccgatc ggcactccga
     1681 agcggcgggc gaaatggccg gcgatgtcgc aatccgacca gccgggcttg ggtgtgtcgg
     1741 tgatgtggcc gtagcggggc gaggcggggt ccagcaccgc cgggccgaag ctgccgacac
     1801 cgatagcggc cagcggcgcc tgggcggaaa accagtcggc cgtggcggcg agggtttcag
     1861 ccggcgtggt ggtgggcagg cggtggctgg caagaacctt gcccgaggcc gtgccgatgg
     1921 cgaggacgaa cttggtgccg ccggcttcca ccgcaccgag gagcgggccg tccccggcgg
     1981 ggatggtcat gcggtggtcc gttcggcgat gcggcagcaa tcgccttcga ccacgagttg
     2041 aagcaggggc gcgggcacgc cgccgaagtg gcgagcgagg gcggcggtgt caaccgggca
     2101 gggcccgccg cgcaggcccc agatatcgac gaaactgctc acgaccagat cggccgagac
     2161 gaaccagctg tagctctgca gcggttcgtc cacggggttg gcgaggacca ggccgctgcc
     2221 gttgagcggg cgccaggggc cggcgaggct atcggcgatc atgccgtaga gcccgctcgg
     2281 cgcgctgcgc agctgggggg agaaagtgct ggtctgggtg acccagaaga cgagatagcg
     2341 gccctggtgg acgacgacgt gggcccgttc gagttcgttg ttgacgccat cggcggtgac
     2401 cagcgggggc aggggctccc agccgtcggc ggtgcgccgg gcaaggccga ccgcgccgtt
     2461 gaacgggctg gatgcgcccg ctacggatgc ggtaaagaca aggtattccg cgccgtcggc
     2521 cggattgcat agccaggcgg gatcgcggaa agccttgatg cggcccggct cgccgtcatg
     2581 agcatcggcg ctctggtaag ccgggccgga agccagcgag agctgcgggg ccgaccagtc
     2641 gcgcggcagg ccgtccgggc cgataatggc gtgggcttcg tagagggcct gctggtagcc
     2701 accgggcctt tcgctggttc cggcgccggt gaaccagagc gagacgcggg tgccgtcagt
     2761 gctggcggaa ccggcccatt cgcgttcgta ggggaccggg ccatcgggca ggaccgggcc
     2821 gaggtcggtc cagcgttccg cgccgagcgg gccggtgcgt tcaagcaggc ggatcttcgc
     2881 ctcgaaatgg cgcagcgccg ggtcgccgcg gtgcggcgcg ctgagcgcca tccacagttc
     2941 gcgttcgcca agccgggcaa tgtggccggt ggcatcctgg atcggccaca tgtcccagta
     3001 gaccaggccg ggaaggaggt ccgaccgctc agccccggcg atgacgggca cgcgcggagc
     3061 ggtggccggg tcgatggtgc ggacttgggc gggagtccat gcggtggtca tgacaggtct
     3121 ggttttccgg ggagggcatt cagggacaag cggcgggcaa ggggcgggcg gagcggcacc
     3181 tggcgcgccg caccgcccgc cgggggaggc ttagaaatcg tagcgcagcg aggccgagac
     3241 ggttcgtcca ttgatcgacc gggcgcgcac gtagttgttg gcgttgttga cgatggagcc
     3301 ttcctccgcc tcggtgatgc cgacgacgtt gaacaggttg ttggcgttga gcgacagttc
     3361 gacgcccttc atcggccgga cgttcacgaa ggcgttgacc tgggtgtagc cggggaagac
     3421 gaggttgttc tcgtcctggg cataggcctt ggtcgtgcca atgatgttga ggccgacgcg
     3481 ggccacgtcg aaatccagcg acggggtgaa ctggtagatc agctcggctt gccggcgcgg
     3541 ggtgtggccc ttgttggcgg gcgtgatcgc atcggcggcg atgcgggcgt gggtgtaggt
     3601 ggcgccgccg cgcaggtcga aaatgcccga gcgccaggcg gcctcgatct cgacgccggt
     3661 cgccttgtag gtgcgatcga ggaagcggcc gctggtgacc tcgaagttct gttcctgggt
     3721 cgtggcgtgg aagccggtga cgaacaggcc gaacgggccg gagcggtact tgaagcccag
     3781 ttcgtactgg ttgacgtaat cgatcgcgtc gtccttgcgg accgagccgt cgggccggac
     3841 cacgccgaac agcagacgat cggcgttggc gcggccgccg cggctggcgc gggcgaacac
     3901 cgcggcatcg ggcgagacgc ggtagttcgc accgatcgac cacgagacat agccgacgct
     3961 gtagttgacc gggctcggat tggcgttgtc gatcagcgag acgctgcgct cgaccggctg
     4021 gatcgtgccg tcgccgttga cg
//
LOCUS       DATNBV010000171         6481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4247386,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000171 DATNBV010000000
VERSION     DATNBV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4247386"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..955
                     /locus_tag="VN627_04155"
     CDS             110..955
                     /locus_tag="VN627_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HXD02480.1"
                     /translation="MRLDLVDVFANGPLSGNPLAVVRGDVALSEMQMASLTRWLGYSE
                     TAFVLPPVDPAADYRVRIFSPAGELPFAGHPTLGSTFAWLAAGGEPRHPGIVVQECGI
                     GLVEVRRDVGQLSFKAPPLSRSAPLSASERDEAIRIAGLREACVVDAVHACNGPDWQL
                     LRLAALDDLLAAEPMPRARRGTKLGLVAPHLAESRVAYELRAFVADAGGRLFEDPVTG
                     SLHAAAAQHLLASGMAEGAWLAAQGRKVGADGRVHVHVDLRGEVWIGGVVRAVAMDAR
                     LTPLA"
     gene            complement(960..2207)
                     /gene="rlmN"
                     /locus_tag="VN627_04160"
     CDS             complement(960..2207)
                     /gene="rlmN"
                     /locus_tag="VN627_04160"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXD02481.1"
                     /translation="MADTNLMPIPGHIDPVPVPRDVTPRADGRVDLLGLPRPRIADLL
                     AEAGLDAKAAKLRAKQVFHWLYHRGVTDFEAMTDIAKTMRPWLAERFVIGRPEVVVAQ
                     HSEDGTRKWLLRTADDHEFEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNL
                     TPGEIVGQVMLARDELGEWPKGAMQGLDEDEDEGSYTSDGRLLTNIVMMGMGEPLYNF
                     DNVRDALRLVMDGDGLALSKRRITLSTSGVVPMMARAGEEIGVNLAVSLHAVSKEVRD
                     EIVPINRKFGIEELLQACASYPGASNARRITFEYVMLKDKNDSDDDARELVRLIKQYK
                     LPAKVNLIPFNPWPGAIYECSSPERIRRFSEIVFEAGISAPIRTPRGRDIDAACGQLK
                     TAAQKLSRAELDRLAEEKQAALG"
     gene            complement(2244..2660)
                     /locus_tag="VN627_04165"
     CDS             complement(2244..2660)
                     /locus_tag="VN627_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02482.1"
                     /translation="MFGTWGAFRDPAIPRCYAIAMAEPSTLSREFQPYAAIGTWPRQG
                     ARGQVHFRLSRRLAAGRAVTLSIGGRRFALAAGAADAWAQDPAMDAAIVAAMRSAPGM
                     WISAHDAKGHSFGNGYQLDGAATAMDAATVGCARLR"
     gene            2811..3821
                     /locus_tag="VN627_04170"
     CDS             2811..3821
                     /locus_tag="VN627_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HXD02483.1"
                     /translation="MVFDRAARGAELLPHARLLREILDSQLEMVCRFRRDGTILFVNR
                     AYAEAMAQPAEALVGQSLWQFIPPEDRTAVEAGLDQLRPDHPHVNIENRMETAAGIRW
                     TLWRNHGLNWDGQGTLLEAQSTGFDITERKELEEQRQLLIDELNHRVRNTLTVVQGMA
                     HQTFRGSEIPPRQLAAFYARLHALAGAHTALSRSNWAGAEMAEVIRQGLAICGDDIER
                     IAMSGPPAKLRPSAAVALALVLHELATNAVKYGALSDHAGRVAVRWQVGAEDRLAVTW
                     QERGGPPVVPPARTGFGSRLITSSIARQLDGSVDLAFEPEGLTCRLEFPLRSDRTPSD
                     LP"
     gene            3818..4171
                     /locus_tag="VN627_04175"
     CDS             3818..4171
                     /locus_tag="VN627_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXD02484.1"
                     /translation="MITGKRILIVEDEFLVAAMLEDELADAGALVIGPAATLADGLRL
                     ARTGDFDAAVVDWNLDGENSGPIAQALLQRDLPFVVSTGYGAVADEFAGCPLLAKPYD
                     PAELVSLLAGLLGQG"
     gene            complement(4186..5283)
                     /locus_tag="VN627_04180"
     CDS             complement(4186..5283)
                     /locus_tag="VN627_04180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXD02485.1"
                     /translation="MFKFLSRSAEAVEPASAPAQPPHHEGPGRAGRRELLERIGDFLV
                     RHDLDVTGSNLTIAQAAFSGADLALASRIAEQERTGAAIDQAWLDRIAPAEEEDDQAQ
                     AKEAELDRLMTRLDTSISSFAATTRKAGGATAVYGDTLQEQVGAIAETAGGARSDEMV
                     ANLVGIARAMLDRTREIEHEMKRSSQEAESLRESLERARRDAELDHLTGLPNRRAFEA
                     VLQREVREARAEIDNLTVAICDIDRFKSVNDTHGHDTGDRVIQAVAEVLARISNDRCH
                     VARHGGEEFVLLFRGKSVAEAAEMLDQARETMASRHLINRLTDQPIGQVTFSGGVANV
                     FAHGDPRDALRAADQALYQAKADGRNRICRA"
     gene            complement(5395..>6481)
                     /gene="sthA"
                     /locus_tag="VN627_04185"
     CDS             complement(5395..>6481)
                     /gene="sthA"
                     /locus_tag="VN627_04185"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HXD02486.1"
                     /translation="HRNNIRTVSGMARFVSPHEIAVSRTDGSDLAVTAERFLIAVGTV
                     PHRPDCIPFDQCHVVDSDSIVEVPRVPRSLAVIGAGVIGIEYATIFSALDVPVTLIEP
                     RDSFLDFVDREVIGEFVHMLRDRGMTIRLGCSVDRIERGPDGWPVTWLTDGRRIRSDM
                     VLYAAGRKGATGALALDRCGLATDHRGRLEVDPKTFQTAVPHIYAAGDVIGFPSLAST
                     SMEQGRIAACHAFGLPIPPAPAYFPYGIYAVPEISTVGLNEEECRARKIGYEVGIARF
                     RETSRGHIMGLHAGMMKLIFSLKTRRLLGAHIIGEGATELVHIGQAVINLKGTLDYFI
                     ENTFNYPTLAEAYKVAALDAWNRMSAA"
BASE COUNT         1068 a         2044 c         2221 g         1148 t
ORIGIN      
        1 tcttcatctg tccggtgctc ctcaaacggc gggcccagtt aacggccttg ccaaccgata
       61 gtccggccaa gggttccgta acatgaacgg acgtggcacg aagcggcgca tgagacttga
      121 tctggtcgat gtgttcgcga acgggccgct cagcggcaat cctctcgccg tggtcagggg
      181 ggatgtcgcg ctgtcggaga tgcagatggc gtcgctgacc cgctggctcg gctactcgga
      241 gacggccttc gtgctgcccc cggttgaccc cgccgccgac taccgggtgc gcatattctc
      301 gccagctgga gaactgccgt tcgccggaca tcctacgctg ggaagtacct tcgcctggct
      361 ggccgccggt ggggagccac ggcatccggg gattgtcgtt caggaatgcg gcatcggact
      421 ggtcgaggtc cgtcgcgacg ttggccagct gtcgttcaag gcgccgccgt tgagcagatc
      481 cgcgcctctg tcagcatcgg aacgcgatga ggccatccgc atcgccggct tgcgcgaagc
      541 ctgcgtcgtt gatgccgttc acgcctgcaa cgggccggat tggcagctgc tgcgcctggc
      601 cgctctggat gatctgctcg ccgcagagcc gatgccgcga gcccgccgtg gaaccaagct
      661 cggccttgtt gcgccgcacc tggccgaatc gcgggtcgct tacgaattgc gggcgttcgt
      721 agccgacgcc ggcggtcggc tgttcgagga ccccgtgacc ggcagcctgc atgccgcggc
      781 agcccagcat ctgctggcga gcggcatggc agaaggcgct tggctcgcag cacaggggcg
      841 caaggtcggc gctgatgggc gggttcacgt ccacgtcgat ctacgcggcg aggtgtggat
      901 cgggggcgtt gtgcgtgccg tcgccatgga cgcccgattg acgccgctgg cttgagaatt
      961 cagccgagcg cggcctgttt ttcctcggcc agccggtcca actcggcgcg gctcagcttc
     1021 tgcgcagcgg tcttcagctg gccgcaggcc gcatcgatat cgcgcccgcg cggcgtgcga
     1081 atcggagcgc tgatcccggc ttcgaacacg atctcggaaa agcggcggat ccgttcgggg
     1141 ctcgaacatt cgtagattgc cccgggccac gggttgaacg ggatcagatt gaccttggcc
     1201 ggcagcttgt attgcttgat caggcggacc agctcgcgcg catcatcgtc gctgtcgttc
     1261 ttgtccttga gcatcacgta ttcgaaagtg atgcgccggg cattcgaggc cccgggatag
     1321 gaggcgcagg cctgcagcag ctcctcgatg ccgaacttgc ggttgatcgg cacgatctca
     1381 tcgcgcacct ccttgctcac cgcatgaagc gaaaccgcga ggttcacgcc gatttcctcg
     1441 ccggcgcgcg ccatcatcgg gacgacgccg ctggttgaaa gggtgatgcg gcgcttcgac
     1501 agggcgaggc cgtcgccgtc catgaccagg cgcagggcgt cgcgcacatt gtcgaaattg
     1561 tagagcggtt cacccatgcc catcatcacg atgttggtaa gcaggcgtcc gtcggacgtg
     1621 taggagcctt cgtcctcgtc ctcgtccagc ccctgcatcg cgcccttggg ccattcgccc
     1681 agttcgtcgc gcgccagcat gacctgcccg acgatctcgc ccggcgtcag gttgcgcacc
     1741 agccgcatgg ttccggtatg acagaaccgg cagttgagcg tgcacccgac ctggctggat
     1801 acgcacaggg tgccccggtc cgcgtcgggg atgaagacca tctcgaactc gtgatcgtcc
     1861 gccgtgcgca gcagccactt gcgggtgcca tcctcggaat gctgggcaac cacgacctcc
     1921 ggccgtccga tgacgaagcg ttccgccagc cacgggcgca tggtcttggc gatgtccgtc
     1981 atcgcttcga agtcggttac gccgcggtgg tagagccaat gaaacacctg cttggcccgc
     2041 agcttggcgg ccttggcgtc gagcccggcc tcggcgagca gatcggcgat gcgagggcgc
     2101 ggcagtccaa gcaggtcaac gcgcccgtcg gcgcgcgggg tcacgtcacg ggggacgggc
     2161 accgggtcga tgtgcccggg gatcggcatg aggttcgtat cggccatgga ggcgcgcgat
     2221 atagtggcaa ttcccggcaa ttgctagcgc aggcgggcgc agccgaccgt ggcggcgtcc
     2281 attgcggtcg cggcgccgtc cagttgatag ccgttgccga acgagtgacc cttggcatca
     2341 tgggcgctga tccacatgcc gggcgccgaa cgcatggcgg cgacgatggc ggcgtccatt
     2401 gccggatcct gagcccaggc atcggccgcg cccgccgcga gggcaaagcg gcgccctccg
     2461 atcgagagcg tcaccgcccg ccccgctgcg agccggcgag acaggcggaa atgaacctgg
     2521 ccgcgcgcgc cttgccgtgg ccaggtgccg atggcggcat agggctggaa ctcgcgggag
     2581 agcgtcgagg gctccgccat ggcaatggca tagcagcgcg gaatggcggg atcgcgaaag
     2641 gccccccacg tgccgaacat gcccaggctg tcgcgcgcaa gagccggctg gatggcgatg
     2701 atcaagacag cggccagggc acaaaaagag atcggatggg gtgtcacacc caagcatttg
     2761 cagatcgcgg gcaccaacgg aagcctctga cgattgcttt tctcaaaggc atggtgttcg
     2821 atcgggcggc aagaggggct gaactgttgc cgcacgcgag attgcttcgt gagattctcg
     2881 actcccagct cgagatggtc tgtcggttcc ggcgcgacgg gacgatcctg ttcgtgaacc
     2941 gggcctatgc cgaagctatg gcgcagcctg ccgaggcgct ggttggccag agcctgtggc
     3001 agttcatccc gccggaagac cgcacggcag tggaggccgg gcttgaccag cttcgtcccg
     3061 atcatcccca cgtcaatatc gagaaccgga tggaaacggc tgccggcatc cgttggacct
     3121 tgtggcgcaa ccacgggctt aactgggacg ggcagggtac actgctggag gcccaatcca
     3181 ccggcttcga cattaccgag cgcaaggaac tggaagagca gcgacagctg ctgatcgacg
     3241 aactcaatca ccgtgtgcgc aacacgctta ccgtggtcca gggcatggcc caccagacgt
     3301 tccgcggcag cgagatcccg ccccggcaac tggctgcgtt ctacgcccgc ctccacgccc
     3361 tggccggcgc tcacacggcg ctcagccgat cgaattgggc tggagccgaa atggccgaag
     3421 tcatccgcca gggacttgcg atttgcggcg atgacatcga gcggattgca atgtccggcc
     3481 cgcccgcaaa attgcggccc agcgcggcag tggcgcttgc cctcgttctg cacgagctgg
     3541 caaccaatgc ggtgaagtac ggcgccttgt cggatcatgc ggggagagtg gcggttcgct
     3601 ggcaagtcgg ggccgaagac cggctggcag tcacctggca ggagcgcgga gggccgcccg
     3661 tcgtgccccc tgcccgcacc ggcttcggtt cgcgcctgat caccagttcc attgcgcgtc
     3721 agctcgatgg cagcgtggac cttgcctttg agccagaggg gctgacttgc cggctggaat
     3781 ttccgctgcg ctccgaccgg acgccgagtg acctgccatg attactggca agcgtatcct
     3841 gatcgtggag gacgaattcc tcgtcgccgc catgcttgag gacgaactgg ccgatgccgg
     3901 ggcactcgtc atcgggccgg cagcgacact tgccgacggc ttgcggctgg cccgcaccgg
     3961 cgacttcgac gcggctgtcg tggactggaa cctggacggc gaaaattcgg ggccgatcgc
     4021 ccaggctttg ctgcagcgcg acctgccctt cgttgtttcc accggttacg gggcggtggc
     4081 ggacgagttc gccggatgcc cactcctggc caagccctac gaccccgcgg aactggtgag
     4141 cctgctggct gggttgctcg gacaaggttg aggccgtcag gcccttcagg cgcggcagat
     4201 gcgattcctc ccgtctgcct tggcctgata aagcgcctgg tccgcggcgc gcagggcatc
     4261 acgcggatcg ccgtgggcga agacgttcgc aaccccgccg gaaaaggtta cctgcccgat
     4321 cggctggtcg gtcagacgat tgatgagatg gcgcgaggcc atcgtttcgc gggcctggtc
     4381 gagcatctcc gccgcttcgg ccacgctctt gccccgaaac agaaggacga actcctcccc
     4441 gccatgccgg gcgacgtggc accggtcgtt ggaaatgcgg gcaagtacct cggccacggc
     4501 ctggatgacc cgatcgccgg tgtcatggcc atgggtgtcg ttcactgact tgaaccggtc
     4561 gatgtcgcaa atggctacgg taaggttgtc gatctccgcc cgggcttcac gcacctcgcg
     4621 ctggagcacg gcctcgaagg cgcggcggtt cggcagtccg gtcaggtggt cgagctcggc
     4681 gtcgcgccgg gcgcgttcca ggctttcacg cagggattcg gcttcctgcg acgagcgttt
     4741 catttcatgt tcgatctcgc gggtccggtc gagcatggcg cgggcgatac ccaccagatt
     4801 ggcgaccatc tcgtcggacc tcgccccacc ggcagtttcc gcgatcgctc ccacctgctc
     4861 ctgcagagtg tcgccataga ccgcggtagc tccgcccgcc ttgcgggttg tcgcggcaaa
     4921 gctggagatc gaagtgtcga ggcgggtcat caggcgatcg agctcggcct cctttgcctg
     4981 ggcctggtca tcttcttcct cggcgggtgc gatccggtcc agccaggcct ggtcgatcgc
     5041 ggcgccggtg cgctcctgct ccgcaattcg gctggccagg gcaagatcgg caccggaaaa
     5101 ggcagcttgg gcaatggtga ggttgctacc agtcacgtcg aggtcgtggc gtacaaggaa
     5161 gtcgccgatc cgttccagca gttcccgccg cccggcgcgg ccggggccct cgtgatgggg
     5221 gggctgggca ggggccgacg cgggctcgac cgcttcggcc gagcgcgaca ggaacttgaa
     5281 cattgatgac cgtcctcggt ttatttggcg acccgcggat caggctcgat tagaccgtgt
     5341 gtggctgaca ttctcttggc gaggccatcc ggtaatctac gggaggccga tttttcaggc
     5401 agcgctcatc cggttccagg catcgagcgc agcgaccttg taggcttcgg cgagcgtggg
     5461 gtagttgaag gtgttctcga tgaaatagtc gagcgttccc ttcaggttga tcacggcctg
     5521 accgatgtgg acgagctccg tggccccttc gccgatgatg tgggcgccga gcaggcggcg
     5581 ggtcttcagg gagaagatca gcttcatcat gccggcgtga aggcccatga tgtggccgcg
     5641 cgacgtttcg cggaaacggg cgattcccac ttcataaccg atcttgcggg cgcggcattc
     5701 ctcctcgttg aggccgaccg tgctgatctc cggcacggcg tagatgccgt aggggaaata
     5761 ggcgggcgcc ggggggatgg gcaggccgaa ggcgtggcag gcggcgatgc ggccctgctc
     5821 catcgaggtg gaggcgaggc tggggaagcc gatcacgtcg cccgcggcat agatatgagg
     5881 gacggcggtc tggaatgtct tgggatcgac ctccagccgg ccccggtgat cggtggcaag
     5941 accgcagcgg tcgagcgcca gcgccccggt cgcgcctttg cggccggcag catagagcac
     6001 catatcggag cgaatgcggc ggccgtcggt cagccaggtg accggccagc catctggccc
     6061 gcgctcaatc cggtcgaccg aacagcccag gcggatggtc attccgcggt cgcgcagcat
     6121 gtggacgaat tcgccgatca cctcacggtc gacgaaatcg aggaagctgt cgcgcggctc
     6181 gatcagggtg acggggacgt cgagggcgga aaagatcgtg gcatattcga tgccgatcac
     6241 acctgcgcca atgaccgcga ggctgcgtgg cacgcgtgga acctctacaa tgctgtcgct
     6301 gtcgaccacg tggcattgat cgaacgggat gcaatcaggc cggtggggca cggtgccaac
     6361 cgcgatcagg aaccgttctg cggtaacggc aaggtcgctg ccatcggtac gggacacggc
     6421 aatttcatgc ggcgagacaa agcgcgccat gccggaaacg gtgcggatat tgttgcggtg
     6481 g
//
LOCUS       DATNBV010000172         2897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4247455,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000172 DATNBV010000000
VERSION     DATNBV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4247455"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VN627_04190"
     CDS             <1..646
                     /locus_tag="VN627_04190"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HXD02487.1"
                     /translation="ASMNYSLNLGWPLYLSTKNTIMKAYDGRFKDLFEEVYNTEFKAQ
                     FEAAGISYEHRLIDDMVASALKWSGKFVWACKNYDGDVQSDTVAQGFGSLGLMTSVLL
                     SPDGKTVEAEAAHGTVTRHYRMHQQGKATSTNPVASIFAWTRGLMYRGKFDNTPDVVK
                     FAETLERVCVETVESGKMTKDLALLIGPDQNWLTTEGFFQAIVDNLETEMANWA"
     gene            complement(753..1133)
                     /locus_tag="VN627_04195"
     CDS             complement(753..1133)
                     /locus_tag="VN627_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02488.1"
                     /translation="MRKSAYAGITFAFFVVSVLLQWLSGWAEQSAGTAPPDKAANIAQ
                     FTAGLARETFAHWQGGFLQVLWQMLGLGWFLHLGSAATRRQDERVERKLDVLLGYLAL
                     EREREDIDRDGEREASERPEPRIR"
     gene            1245..>2897
                     /locus_tag="VN627_04200"
     CDS             1245..>2897
                     /locus_tag="VN627_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXD02489.1"
                     /translation="MSADLHTPALSDALVILGAAGLVIPIFARFRITPVIGFILVGLL
                     VGPHGLGGRVGEQPWLYHVTISDPGGLAPFAEFGIILLLFSVGLELSFARLWAMRREV
                     FGVGAAELLGSAALIGTGLAAMGQAWPTALALGLALALSSTALVLKITDMRTPVGRAA
                     LSMLLFEDIALVPIIFLLGALGGRGDAGGSSAILTTLGLGSLAVAGLLVAGRQLLPGL
                     FAQAARTKSPELFLSASLLVVIIAALVTSATGLSPIVGALIAGLLIAETEYHGEVEAM
                     TEPFKGLALGIFLITVGMQLDLGLIWSDFGRIVAALALVFFGKAVVTALMLRLSGARR
                     GTSTEAGLLLASPSETTLIVLSAGLSAGMLTGEVAQFWQTVTALGLTVTPIMALIGRW
                     AARRVDRGSLRPADNEDEGQRSLIIGFGRVGRMVADMLDEHDQPYRAIDADADLIAAA
                     QRDGYAAQYGDAARPGQLGRLGLDGASAVILTMDDPQGALRLVRRIRKENAGVPIIAR
                     ARDAVHAGQLYRAGATHAVPETLESSLQLSEAVLVDLGVPMGF"
BASE COUNT          437 a          992 c          958 g          510 t
ORIGIN      
        1 cgcctcgatg aactactcgc tcaacctggg ctggccgctg tatctctcga ccaagaacac
       61 gatcatgaag gcctacgacg ggcgcttcaa ggacctgttc gaggaggtct acaacaccga
      121 gttcaaggct cagttcgagg ccgccggcat ctcctacgag caccgcctga tcgacgacat
      181 ggtcgcctcg gcgctcaagt ggtcgggcaa gttcgtctgg gcctgcaaga actacgatgg
      241 cgacgtccag tccgacaccg tggcccaggg ctttggctcg ctgggcctga tgacctcggt
      301 cctgctctcg cccgacggca agacggtcga agccgaagcc gcccacggca ccgtgacccg
      361 ccactaccgc atgcaccagc agggcaaggc gaccagcacg aacccggtcg cctcgatctt
      421 cgcctggacc cgcgggctca tgtaccgcgg caagttcgac aacacgcccg acgtggtgaa
      481 gtttgccgag accctcgagc gcgtctgcgt ggagacggtg gaaagcggca agatgaccaa
      541 ggacctcgcc ctgctgatcg gccccgatca gaactggctg acgacggagg gcttcttcca
      601 ggccatcgtc gacaatctcg agaccgaaat ggccaactgg gcctgacgcg ttgggcggct
      661 gcgcgggtta gttctcgcag ccgtccccgt ttcctccggt gaaggctgtt gcaaccacgt
      721 gtccgtcggg tgcacgctga agcgagccga agtcaccgga tacgaggctc agggcgttcg
      781 ctggcttccc gctcgccgtc acgatcgatg tcctcgcgtt cccgttccag agcaaggtag
      841 ccgagcagga cgtccagctt gcgttccacc cgctcgtcct gccggcgcgt ggcggccgaa
      901 ccgagatgca ggaaccagcc gagcccgagc atctgccaca acacctgcag gaaccctcct
      961 tgccagtgag cgaaggtctc gcgcgccaag ccggcggtga actgcgcgat gttcgctgcc
     1021 ttgtccggcg gcgcagtgcc agcggattgc tcggcccacc cgcttagcca ttgcagcagg
     1081 acggaaacga cgaaaaacgc gaaagtgatt cccgcgtagg cagacttgcg catggacgac
     1141 cccgatttga ggttggccta tgcaattaag taactgagtt gtcatgatta atttcgcgta
     1201 gccgcgcccg tgacaaggac tgcacccttc cctaccctgc gccgatgtcc gccgacctgc
     1261 acacccccgc cctgtccgat gcgctggtga tcctgggggc tgcggggctg gtgatcccga
     1321 tcttcgcgcg gttccggatc accccggtaa tcggattcat tctcgtcggc ctgctggttg
     1381 ggccgcatgg cctaggcggt cgggtgggcg agcagccctg gctctaccac gtgacgatct
     1441 ccgatcccgg cggcctcgcg ccgtttgccg agttcgggat catccttttg ctgttctcgg
     1501 tcggcctgga gctgtcgttc gcccgcctct gggccatgcg ccgggaggta ttcggcgttg
     1561 gcgcggcaga actgctgggc agcgcagcac tgatcggcac aggcctggcc gccatgggcc
     1621 aggcctggcc aacggcgctg gcgctggggc tggccctcgc gctctcgtcc accgcgctgg
     1681 tactcaagat caccgatatg cgcacgccgg tcggccgggc ggcgctgtcg atgctgttgt
     1741 tcgaggatat cgcgctggtg ccgatcatct tcctgctcgg cgcgctgggc ggccgcggcg
     1801 atgccggcgg ttcctcggca atcctgacca ccttgggact gggctccctc gccgtcgccg
     1861 gcctgctcgt ggccgggcgg cagctgctcc ccggcctgtt tgcccaggcg gcgcggacca
     1921 agagcccgga actgttcctc tcggccagcc tgctggtggt gatcattgcc gcgctggtca
     1981 cgtcggcgac cggcctttcg ccgatcgtcg gcgcgctgat cgccgggctg ctcatcgccg
     2041 aaacggaata tcacggcgag gtcgaggcga tgaccgaacc gttcaagggc ctggcgctcg
     2101 gcatattcct gatcacggtc ggcatgcagc tcgacctggg gctgatctgg agcgatttcg
     2161 ggcgcatcgt ggccgccctg gcgctggtgt tcttcgggaa agccgtggtc acggcgctga
     2221 tgctgcgcct ctcgggcgcg cggcggggta cctcgaccga ggccggcctg ctgctcgcca
     2281 gcccctcgga gacgacgctg atcgtccttt ccgccgggct gtcggcgggc atgctgaccg
     2341 gcgaagttgc gcagttctgg cagacggtca ccgcgcttgg gttgaccgtg acccccatca
     2401 tggccctgat cggccgctgg gcggcgcggc gcgtggaccg gggcagcctg cgcccggccg
     2461 acaatgagga cgagggccag cggtcgctga tcatcggctt tggccgcgtc ggccggatgg
     2521 tcgccgacat gctggacgag cacgaccagc cctaccgcgc gatcgatgcc gatgccgatc
     2581 tcatcgccgc ggcccagcgc gacggctatg cggcgcagta tggcgacgcc gcccgccccg
     2641 gccagctggg ccggctcggc ctggatgggg cgagcgcggt catcctcacc atggacgatc
     2701 cccagggggc gctgcggctg gtccgccgga tccgcaagga gaacgccgga gttccgatca
     2761 tcgcccgcgc ccgcgatgcc gtccatgccg gccagctcta tcgggccggg gccacccatg
     2821 ccgttcccga aacgctggaa agctcgctcc aactctccga agccgtgctg gtcgacctcg
     2881 gcgtgccgat gggcttc
//
LOCUS       DATNBV010000173         4034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_747304,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000173 DATNBV010000000
VERSION     DATNBV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747304"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VN627_04205"
     CDS             complement(<1..502)
                     /locus_tag="VN627_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HXD02490.1"
                     /translation="MSTEVTGGAVAIPVRRDDYTGAGGQTTRAVPLEVPIAIELNGVA
                     YAVMMATPLDLADFITGFLLAEGLAEAGEIEEPSFHPVEGEAGCGIVARLNLPAHRAA
                     PLMERARRRLGDSSCGICGIESVELALRPLPRLPAAPPIASAAIRRGLAAMREAQVLG
                     RETAATH"
     gene            complement(499..2013)
                     /locus_tag="VN627_04210"
     CDS             complement(499..2013)
                     /locus_tag="VN627_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HXD02491.1"
                     /translation="MTVVRISDDALALACGADAVAAAFAAAGCTVERVSSWGMHWLEP
                     LVEIDGQGFGPASPADAAAILDGTSTKAIGPVAAHPFLARQYRLTFARAGRTRPLSLD
                     DYAATGGWAGLDRARGMAPEQVVAEVIASGLRGRGGAGFPAGIKWQTVAGAPGTRKYV
                     VCNADEGDSGTFADRMVMEGDPFQLLEGMAIAAHAVGAGQGYIYVRSEYPHAIAKLRA
                     AIDLAAPMLAPFAIEVRVGAGAYVCGEETSLLNSLEGKRGEVRAKPPLPALQGLFGRP
                     TVVNNVLTLTAVAHILAPGGAEAYAALGMGRSKGSMPVQLAGNIRHGGLFEVPFGITL
                     GELVHEIGGGTASGRPVKAVQVGGPLGAYIPPSQFDLPFDYEAFAAADALIGHAGVVV
                     FDDSADMGRMARFAMEFCAAESCGKCTPCRLGSTRGVELLDRIRAGTPLADPVAALPQ
                     MHNAPKVPRSREADLALLEDLCETMQFGSLCALGGFTPYPVRSALRHWPDDFLP"
     gene            complement(2010..2429)
                     /locus_tag="VN627_04215"
     CDS             complement(2010..2429)
                     /locus_tag="VN627_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXD02492.1"
                     /translation="MPDLDQIIAAHANREGPLLPILHDVQHAFGCVSPEAERAIALAL
                     NLSRAEVHGVVSFYHDFTATPDPRPCVQLCRAEACQSRGVEALVPAAQAAAGDRVRLA
                     TVYCLGLCSAGPAARVGDRVHARLDEAALVQLVEAAR"
     gene            complement(2468..4033)
                     /locus_tag="VN627_04220"
     CDS             complement(2468..4033)
                     /locus_tag="VN627_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXD02493.1"
                     /translation="MVIYMPMIPEAVFAMLACARLGAIHSVVFGGFAAHELAKRIDDA
                     KPSVILTASCGIEGARTIAYKPMVDEARDLASHQVEHVVVHQRERLQAELGARGDCDW
                     TALRAETAAEPIPAPVPVAADDPLYILYTSGTTGVPKGVVRENGGHAVALAWSMANVY
                     GVGPGEVFFSASDIGWVVGHSYIVYAPLLVGATTVLFEGKPVGTPDAGTFWRVIDKHK
                     VKAMFTAPTAIRAVRKEDPEAKFLHEIGTGPLKAVFLAGERADPETIAWLEGHTGLPV
                     IDNWWQTELGWPAIASCFAMGDTRRKPGSAGFPVPGYEFAILGEDGHPVPDGQSGAVA
                     IRAPLPPGAFRTLWNNPAGFARTFASFPGWYETGDAGFRDEQGFLHIMGRTADIINVA
                     GHRLSTGQMEQIVAAADGVAECAVVGADNALKGQVPVAFVVPRAGFEQDETMAARVIA
                     LVREDIGAVAALRTCHVVAQLPKTRSGKILRNVLRKLAAGEQVATPPTIDDPAIIPAI
                     REVIASERTFRAG"
BASE COUNT          651 a         1354 c         1472 g          557 t
ORIGIN      
        1 catgtgtggc ggcggtctcg cggcccagaa cctgggcttc ccgcatcgcc gccaggccgc
       61 ggcgtatcgc ggccgaggca atgggtggcg cggctggaag gcggggcagc gggcgcaggg
      121 caagctcgac gctttcgatg ccgcagatgc cgcaggaact gtcgcccagc cggcggcgcg
      181 cccgctccat cagcggggcc gcgcggtggg cgggcaagtt gagccgggcg acgataccgc
      241 agccggcctc gccctcgacc ggatgaaacg aaggctcctc gatctcaccg gcttcggcga
      301 ggccttcggc aagaaggaag ccggtgatga agtccgcaag gtcaagcggg gtcgccatca
      361 tcacggcgta ggcgacgccg ttgagctcga tggcgatcgg cacttccagc ggcaccgccc
      421 gggtggtctg gccgcccgcc ccggtatagt cgtcccggcg gaccgggatc gctaccgccc
      481 cgccggtgac ctcggtgctc acggcaggaa atcgtccggc cagtgacgca gggccgagcg
      541 gacgggatag ggcgtaaacc cgcccagcgc gcagagcgag ccgaactgca tggtctcaca
      601 caggtcttcc agcagggcga gatcggcctc gcggctgcgg gggaccttgg gcgcgttgtg
      661 catctgcggc agggcggcca ccgggtcggc cagaggcgtg ccggcgcgga tgcggtcaag
      721 cagctcaacg ccgcgggtcg agccgaggcg gcagggcgtg cacttgccgc agctttcggc
      781 ggcgcagaat tccatggcga agcgggccat gcggcccatg tcggcactgt cgtcgaacac
      841 gaccacgccg gcatggccga tcagcgcatc ggcggcggcg aacgcctcgt agtcgaacgg
      901 caggtcgaac tgggagggcg ggatataggc gccgagcggc ccgccaacct ggaccgcctt
      961 gaccgggcgg ccgctggccg tgccgccacc gatttcatgg accagttcgc caagggtgat
     1021 gccgaagggg acctcgaaca ggccgccgtg gcggatgttg ccggcgagct ggaccggcat
     1081 ggagcccttc gaccggccca tgccgagcgc ggcataagcc tctgctccgc cgggcgcgag
     1141 gatgtgggca acggcagtca gcgtcagcac gttgttgacc acggtggggc ggccgaacag
     1201 gccctggagg gcaggcaagg gcggcttggc gcgaacctca ccgcgctttc cttcgagcga
     1261 attgagcaac gaggtttcct caccgcagac ataggccccc gcgccgaccc gcacttcgat
     1321 ggcgaagggc gcgagcatgg gcgcggccag atcgatcgcc gcgcgcagct tggcgatggc
     1381 gtggggatat tcgctgcgga catagatgta gccctgcccg gccccgaccg cgtgggcggc
     1441 gatggccatg ccttcgagca gctggaacgg atcgccttcc atcaccatgc ggtcggcaaa
     1501 agtgccgctg tcgccctcgt cggcattgca gacgacatac ttgcgggtgc ccggggctcc
     1561 ggcgacggtc tgccacttga tcccggcggg gaagcccgcc ccgccccggc cacgcaggcc
     1621 cgaggcgatg acttcggcga cgacctgctc gggcgccatg ccgcgcgccc ggtccagccc
     1681 ggcccagccg ccggtcgcgg catagtcatc taggctcaac ggccgcgtac ggccggcgcg
     1741 ggcgaaagtc agcctgtact gccgcgcaag gaacgggtga gcggcgaccg gaccgatcgc
     1801 cttggtgctc gttccgtcga ggatcgcggc ggcatcggcc ggggaggccg ggccgaagcc
     1861 ctgcccgtcg atctccacca gcggttcgag ccaatgcatg ccccagctgg agacgcgctc
     1921 cacggtgcag ccggcggcag caaacgcagc ggccacggca tccgcgccgc aggcgagcgc
     1981 aagggcatcg tcgctgatgc gcacgacagt catcgggcgg cctccaccag ctggacgagc
     2041 gccgcttcat cgagccgggc atggacacgg tcgcccaccc gggcggcggg gcccgcgctg
     2101 cacaagccga ggcaatagac ggtggcgagg cgcacccggt cacccgcggc ggcctgcgcc
     2161 gccggaacca gggcttcgac cccgcgcgac tggcaggctt cggcccggca cagctggaca
     2221 caagggcgcg gatcaggcgt ggccgtgaaa tcgtggtaga agctgaccac gccgtggacc
     2281 tcggcgcgcg acaggttgag cgcaagcgca atcgcccgct ccgcctcggg cgagacgcag
     2341 ccgaaggcat gctgcacatc gtgcaggatc ggcagcaggg gaccctcgcg attggcgtgg
     2401 gcggcgataa tctgatcaag gtccggcatg gtcacggcgc gacgcttgcg ccgggacgcg
     2461 gcgagcgtca acccgcgcgg aaggttcgct cgcttgctat cacctcgcgg atggccggga
     2521 tgatcgcggg atcgtcaatc gtcggcggag tggccacctg ctccccggcg gccagcttgc
     2581 gcagcacgtt gcgcaggatc ttgcccgaac gggtctttgg cagctgggcg acgacatggc
     2641 aggtgcgcag cgcggcaacc gcgccgatat cctcgcgcac gagggcgatc acccgggcgg
     2701 ccatggtctc atcctgctcg aacccggcgc gcggcacgac gaaggcgacc ggaacctggc
     2761 ccttgagcgc attgtcggcg ccgaccacgg cgcattcggc cacgccatcg gcggcggcga
     2821 cgatctgctc catctggccg gtggagaggc ggtggcccgc gacattgatt atatcggcgg
     2881 ttcggcccat gatgtggagg aaaccctgct catcgcgaaa gcccgcgtcg ccggtttcat
     2941 accagcccgg gaagctggcg aaggtcctgg cgaagcccgc cgggttgttc cacaaggtgc
     3001 ggaaggcgcc cggcggcagc ggggccctga tcgccacggc gccgctctgg ccatcgggca
     3061 cggggtgtcc gtcctcgccg aggatcgcga actcgtatcc cggcaccggg aaaccggcag
     3121 agccgggctt gcggcgggta tcgcccatgg cgaagcagga ggcgatggcc ggccagccga
     3181 gctcggtctg ccaccagttg tcgatcaccg gcaggccggt gtggccttcg agccaggcga
     3241 tggtttcggg atcggcgcgt tcgccggcga ggaacacggc cttgagcgga ccggtgccga
     3301 tctcgtgcag gaacttggcc tccggatctt ccttgcgcac ggcgcggatg gcggtcggcg
     3361 cggtgaacat ggccttgacc ttgtgcttgt cgatgacccg ccagaaggtg ccggcatcgg
     3421 gcgtacccac cggcttgcct tcaaacagga ccgtggtcgc gcccaccagc agcggggcgt
     3481 agacgatgta gctgtggccc acgacccagc cgatgtcgga ggcggagaag aacacctcgc
     3541 ccgggccgac gccgtagaca ttggccatcg accaggcgag cgccacggca tggccgccgt
     3601 tttcgcgcac gacgcccttg gggacaccgg tcgtgcccga agtgtagagg atatagagcg
     3661 gatcgtcggc ggcgacggga acgggcgcgg gaatgggttc ggcggcggtt tccgcccgca
     3721 atgcagtcca gtcgcaatcg ccccgggcgc cgagttcggc ctggagccgc tcacgctggt
     3781 ggaccacgac atgctcgacc tgatgactgg cgaggtcccg cgcctcgtcg accatcggct
     3841 tataggcgat ggtccgcgcg ccctcgatac cgcaggaggc ggtgaggatg acgctgggct
     3901 tggcatcgtc gatccgcttg gcgagttcat gcgcggcgaa cccgccgaaa acgaccgaat
     3961 ggatcgcgcc gagccgggcg caggcgagca tggcgaagac tgcctcgggg atcatcggca
     4021 tgtagatcac cacc
//
LOCUS       DATNBV010000174         4245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1367726,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000174 DATNBV010000000
VERSION     DATNBV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367726"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VN627_04225"
     CDS             complement(<1..618)
                     /locus_tag="VN627_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HXD02494.1"
                     /translation="MTGSKTGYRGRRVKILATVGPASRSPEMIARLLRAGVDAFRVNM
                     SHGDHATHAETIATIRAAEKDFGRPVAILCDLQGPKLRVGTFANGRERIRHGAHFTLD
                     RDPAPGTEERVCLPHPELFGVLKAGQRLLIDDGKVQLRVIKAGADAILTTAEVGGFIS
                     DRKGVNVPDAVVPVPALTEKDRRDLAFAIEHHADWIALSFVQRPED"
     gene            688..1023
                     /locus_tag="VN627_04230"
     CDS             688..1023
                     /locus_tag="VN627_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HXD02495.1"
                     /translation="MSCSENTNSPDHDPLDALDDTVAAAAFRRLVRHLRHRHDAQNID
                     LMGLAGFCRNCLADWIVEAGAPMDKTEAREAIHGMPAPEWKARFQTEATPEQLARMEE
                     SLKKNGGTH"
     gene            1140..1376
                     /locus_tag="VN627_04235"
     CDS             1140..1376
                     /locus_tag="VN627_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HXD02496.1"
                     /translation="MAESADDRLRLLIERVERLEEEKKGIQDDIKDVYAEAKATGYDA
                     KIMKQIVRIRKMKPDDRREMEAILQTYMAALGIE"
     gene            1621..2481
                     /locus_tag="VN627_04240"
     CDS             1621..2481
                     /locus_tag="VN627_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD02497.1"
                     /translation="MDKSAEFTPPVHEDGLPGSEAGLNPRPDYAPRYPGSGRLAGKVA
                     IVTGADSGIGRAVAVLFAREGARVAIAYLCEDQDAHETLEAIEKEGSEGLLLSGDLGD
                     PEHAAAVVRDTVARWGRLDVVVNNAGEQHPDQDIRDITAEQLQRTFQTNIFAMFYLVQ
                     AAREHLKPGAAIINCTSVTMYQGSRELLDYSSTKGAITAFTRSLSENLVGEGIRVNAV
                     APGPIWTPLNPSGGASPEKLEHFGESTPMKRPGQPNEVAPCFLFLACEDSSYMSGQVL
                     HPNGGTIVNG"
     gene            2551..3315
                     /locus_tag="VN627_04245"
     CDS             2551..3315
                     /locus_tag="VN627_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXD02498.1"
                     /translation="MAGHSKFKNIMHRKGAQDKKRAAQFSKLSREITVAAKMGMPDPD
                     MNPRLRAAVLAARAQSMPKDNIQRAIDKASKGDAENYEEVRYEGYGPGGVALIVEALT
                     DNRNRTATNVRTAFAKNGGNLGASGAVSHGFERLGLIEYPGKAGDEEKVLEAAIEAGA
                     DDVESDLGDGDENPGLHQIWTSVENLHEVAKALEAVLGDAEAVKLVWRPNLKADVDEA
                     TAATLIRLIDVLEEDDDVQTVWGNYEISDEIMEKLG"
     gene            3403..3753
                     /locus_tag="VN627_04250"
     CDS             3403..3753
                     /locus_tag="VN627_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HXD02499.1"
                     /translation="MWQLAAKALLSGALIAAIAEIGKRLPALGALVASLPLVSVLGMV
                     FLWHARPDAENMAVHAEATFWYVLPSLPMFLLIPVLLRHGLPFWAALLAGCLLTVLLY
                     LGMTNFGPRLGLKI"
     gene            3755..4231
                     /gene="ruvC"
                     /locus_tag="VN627_04255"
     CDS             3755..4231
                     /gene="ruvC"
                     /locus_tag="VN627_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXD02500.1"
                     /translation="MLILGLDPSLTCTGWGIIAKSGNRLTHVANGQVRTDPSASLPDR
                     LVTLDRELTDVILAHRPDCAAVEEVFVNKNPQSTLKLGQARGVALLAAARAGLPVSEY
                     ATRLVKKAIVGTGGAEKAQVQAMLKVLLPAAKVTGADAADALAVAIAHAHLLGAHR"
BASE COUNT          771 a         1569 c         1292 g          613 t
ORIGIN      
        1 gtcctcgggc cgctggacga acgagagcgc gatccagtcg gcatgatgct cgatcgcgaa
       61 ggcaaggtcg cggcggtcct tctcggtcag cgccggcacc ggcaccaccg catcgggaac
      121 gttgacgccc ttgcggtcgg agatgaaccc gccgacctcg gccgtggtca ggatcgcatc
      181 ggccccggcc tttatcaccc gcagctggac cttgccgtcg tcgatcagca ggcgctgccc
      241 cgccttcagc acgccgaaca gctccgggtg gggcaggcag acgcgctcct ctgtgccggg
      301 tgcaggatcg cggtcgagcg tgaaatgggc gccgtggcgg atccgctcgc gcccgttggc
      361 aaaggtgccc acgcgcagct tggggccctg caggtcgcac aggatcgcca ccggccgccc
      421 gaaatccttc tccgccgccc ggatcgttgc gatggtctcg gcatgcgtgg cgtggtcgcc
      481 atggctcatg ttgacgcgga acgcatcgac cccggcccgc agcaaccgcg cgatcatctc
      541 cgggctccgg ctggccgggc cgacggtggc cagaatcttg accctccgcc cgcgatatcc
      601 tgtcttcgaa ccggtcacgt tttatccttc cgctgcgccc atcgcctatg gcaagggtga
      661 ggccaaaccc caaggactca aagcccgatg agctgctccg aaaatacgaa ttcaccggac
      721 cacgatccgc tcgacgcgct cgacgatacc gtggccgccg ctgcctttcg ccgcctcgtc
      781 cgccacctgc gtcatcgtca cgatgcccag aacatcgacc tgatgggcct cgccggcttc
      841 tgccgcaact gcctggctga ctggatcgtc gaagccggcg cacccatgga caagaccgag
      901 gcgcgcgagg cgattcacgg catgcccgcc cccgaatgga aggcccgctt ccagaccgag
      961 gcgacgccgg aacagctcgc ccgcatggaa gaaagcctga agaagaacgg cggcacgcac
     1021 tgatcctccc ccctccttgt cccggggagg atttccacag ccgcgctttt gcccgaatct
     1081 ccccggccag ctaatcgccc cgccggttca ccgattctaa cttccggagc ttcatgacca
     1141 tggccgaatc cgccgacgac cgcctgcgcc tgctgatcga acgcgtggag cgtctcgagg
     1201 aagagaagaa gggcatccag gacgacatca aggacgtcta cgccgaagcc aaggccaccg
     1261 gctacgacgc caagatcatg aagcagatcg tccgcatccg gaagatgaag cccgacgatc
     1321 gccgcgagat ggaagccatc ctgcagacct acatggccgc gctcgggatc gagtgacgtc
     1381 gctggggaaa gcctatccgg gctttttgcg gcaccagccc gctcccccgg cccaaccacc
     1441 cacgaggata tgctgtcggg tggtcgggcc gggggagcgg gctggtgccg caagccctcc
     1501 acggatgtgg agggcgcacc aaccaaagac tcggatcggg tgagcgcata gcgcgcctgc
     1561 ccgcggaacg accccaacgc agtgcggttg caatcccgtt gtaaatggga gcgaaccgcc
     1621 atggacaaat ccgccgaatt cacgcccccc gtccatgaag acggcctgcc cggcagcgaa
     1681 gcgggcctga acccccggcc cgactacgcc cccaggtacc ccggctccgg ccgcctcgcc
     1741 ggcaaggtcg ccatcgtcac cggcgccgac agcggcatcg gccgcgccgt cgccgtgctc
     1801 ttcgcccgag agggggcccg cgtcgccatc gcctacctgt gcgaggacca ggacgcccac
     1861 gaaacgctcg aagccattga aaaggaaggc agcgaaggcc tgctcctgtc cggcgacctc
     1921 ggcgatcccg agcacgccgc cgccgtggtg cgcgacaccg tcgcccgctg gggccgcctc
     1981 gacgtggtgg tcaataacgc cggcgagcag caccccgatc aggacatccg cgacatcacc
     2041 gccgagcagc tccagcgcac cttccagacc aacatcttcg cgatgttcta cctcgtccag
     2101 gccgcccgcg agcacctgaa acccggcgcc gcgatcatca actgcacctc ggtgacgatg
     2161 tatcagggct ccagggaact gctcgactat tccagcacca agggcgcgat cacggccttc
     2221 acccgctcgc tctcggaaaa cctcgtcggc gagggaatcc gcgtcaacgc cgtcgccccc
     2281 ggcccgatct ggactccgct caacccctcg ggcggggcga gccccgagaa gctcgagcac
     2341 ttcggcgaga gcacgcccat gaagcgcccc ggccagccca acgaggtcgc cccctgcttc
     2401 ctgttcctcg cctgcgagga ttccagctat atgtcgggcc aggtccttca ccccaacggc
     2461 gggaccatcg tgaacgggtg agattgcccg gaaggccggt ctcggctagg ggcgctggca
     2521 atcgccacac ccaacccgag cgaaaagaaa atggccggcc attccaaatt caagaacatc
     2581 atgcaccgca agggcgcgca ggacaaaaag cgcgctgcgc agttctccaa gctctcccgc
     2641 gaaatcaccg tcgcggcgaa gatgggcatg cccgatcccg acatgaaccc gcgcctgcgc
     2701 gccgcggtcc tcgccgcccg cgcccagtcg atgcccaagg acaatatcca gcgcgcgatc
     2761 gacaaggcca gcaaaggcga tgccgagaac tacgaggaag tgcgctatga gggctatggc
     2821 cccggcggcg tcgccctcat tgtcgaggcg ctgaccgaca accgcaaccg caccgccacc
     2881 aacgtgcgca ccgctttcgc caagaacggc ggcaacctcg gtgcgtcggg ggcggtgagc
     2941 cacggcttcg agcgcctcgg cctcatcgaa taccccggca aggccggtga cgaggaaaag
     3001 gtcctcgaag ccgcgatcga agccggcgcc gacgatgtcg agagcgatct gggcgatggc
     3061 gacgaaaacc ccggcctgca ccagatctgg accagcgtcg aaaacctcca cgaagtcgcc
     3121 aaggcgctcg aagccgtgct cggcgacgcc gaggcggtca agctcgtctg gcgccccaac
     3181 ctcaaggccg atgtggacga agccaccgcc gccaccctga tcaggctgat cgacgtgctg
     3241 gaggaagacg acgacgtcca gaccgtctgg ggcaactacg agatctcgga cgagatcatg
     3301 gaaaagctgg gttaatcctc cccgggacgg agagggggac cacgcacagc gtggtggagg
     3361 ggcaggcgcc gttcttcacc acccaaccgg agaacgcccg caatgtggca actcgccgcc
     3421 aaggccctgc tctccggcgc gctgatcgcc gccatcgccg aaatcggcaa gcgcctcccc
     3481 gcgctcggcg ccctggtcgc ctcgctgccg ctggtctcgg tgctgggcat ggtgttcctc
     3541 tggcatgccc gcccggatgc ggaaaacatg gccgtccacg ccgaggcgac cttctggtac
     3601 gtcctcccca gcctgccgat gttcctcctg atcccggtac tgctgcgcca cggactgccg
     3661 ttctgggccg cgctcctcgc cggctgcctg ctcaccgtcc tgctctacct cggcatgacc
     3721 aacttcggtc cgcgcctcgg cctcaagatc tgacatgctg atcctgggcc tcgacccctc
     3781 gctcacctgt accggctggg ggatcatcgc caagtcgggc aaccggctga cccatgtcgc
     3841 caacggccag gttcgcaccg atccctcggc ctccctgccc gaccgcctgg tcacgctcga
     3901 tcgcgagctc accgacgtga tcctggcgca ccggccggac tgcgcggcgg tggaagaggt
     3961 cttcgtcaac aagaaccctc agtccaccct caagctcggc caggcccgcg gcgtcgccct
     4021 cctcgctgcc gcccgcgccg ggctgcccgt ctccgaatat gccacccgcc tcgtcaagaa
     4081 ggcgatcgtc ggcaccggcg gggcggaaaa ggcgcaggtc caggccatgc tcaaggtcct
     4141 cctgcccgct gcgaaagtga ccggggccga tgccgccgat gccctcgccg tcgccatcgc
     4201 ccacgcgcac ctgctcggcg cccaccgctg acgaccgaac gctcc
//
LOCUS       DATNBV010000175         8296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1369100,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000175 DATNBV010000000
VERSION     DATNBV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369100"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1239)
                     /locus_tag="VN627_04260"
     CDS             complement(7..1239)
                     /locus_tag="VN627_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HXD02501.1"
                     /translation="MLRPFHLGWQSTTAQGDRSRRLVEDIAALSERAASAEYRRVLAD
                     FNERHWQTEAMFDQRYREVEDVLRIDPARFSRSRKRLIGAYFCHEYTYAAAALMNPSI
                     VPHPDQTGMQNGACRFVMSLRAVGEGHISSIAFREGIAEANGSFTLWPQSSFATAVEL
                     DNADLACVDCAVGVHRHEDSSLSNTVIFPITEQQRGGLEDLRLVRFCHDDGTYEWIGT
                     YTAYSGSSIRSELLRTFDFKRFELEPILGAAGRNKGMALFPQKIGGQYMMLGRQDGKN
                     LYLLRSDRLERWEEEGSLLLEPKYPWEFIQIGNCGSPVLTDAGWLVITHGVGAMRKYA
                     LGAALLDRDDPSRVIGRTKEPILTAVDADRSGYVPNVVYTCGVMPVGERLLVPYGISD
                     SAVGFATCLISDLLALMA"
     gene            complement(1271..3649)
                     /locus_tag="VN627_04265"
     CDS             complement(1271..3649)
                     /locus_tag="VN627_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXD02502.1"
                     /translation="MHSDAFRPLRSGEPGTADIVPIFSAPTVPSPRDIARPARRVALV
                     GSYAPRKCGIATFTRDVVEQFARYHPEIALDVYALDNPETAPVTYSGVAGVIPQEDAE
                     AYLRAARAINESGADALWLQHEYGIYGGEDGAMVCDFIDRVAAPLVLTCHTVLAQPSP
                     EQERVLRHLVSRASRIMVMSSHARELLVRRYGASAAVVEVIPHGAPDRPFGRKEQFKG
                     EMGLAGRKVLMTFGLLGPGKGLERVIEALPAIVGRHPEVLYRIVGATHPNCVLHEGER
                     YRDSLAELARRLGVEDHIAWDNRFLATDELLDQLEACDIYVTPYPNLQQATSGTLSYA
                     VALGKAVVSTPYVHATELLADEIGVLIEPGSSAAIAEAVSALLDRPERLAAIERRAYA
                     RGRTTIWPEFADATAGLVARAAAPPAREVPLTVLPGLSGVLTMSDATGMLQHGFGVVP
                     DRRHGYCLDDNARALMLMNVAQGLSRSERMRWSTTYASFVQHAWNPELRRFRNFMNFE
                     RTWCEEAGSDDSNGRALWALGHTVENAPDPDFRRWAQVWFDEVCPVFIDGLDWPRTQA
                     FAMLGAAAMLRADPGHGAARELLERGGSLLHALLEAGRRPDWAWFEAMLGYDNPRLSQ
                     ALIEAGALLGRADWTRAGLETLGWIAGRQTAAAGHFRPIGSETFGLTHASLPFDQQPL
                     EAQAAIEAARSAWDVTGERCWLDHALSAHRWFFGANDRGAVLADIATGRSRDGVTPRG
                     ANENCGAESILAFQLAHYSMLALWSGARAAAERTGDILEPGSRSVSQPFANP"
     gene            complement(3840..4562)
                     /locus_tag="VN627_04270"
     CDS             complement(3840..4562)
                     /locus_tag="VN627_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXD02503.1"
                     /translation="MSEADLRAPRPVRLAAMVMAGLLHLAAAVLLVRAFAPGFTAEVA
                     RRVVSTFTVTVTAPEPEPKPAPAAAAPEAAGAAGVAGDKARPRAVAAPVPKIVLAKPP
                     APPVAGTGSADRAGAALGSGTGAGGPGSGTGAGGSGSGSGGGGGTPLAKIAGDINSAR
                     DYPIASRALRLGDYVEIWLTVGTDGRPSACKVARASRDAAADAITCRLAMERFRFRPA
                     LDASGAARIATYGWRQRWFLKD"
     gene            complement(4608..5165)
                     /locus_tag="VN627_04275"
     CDS             complement(4608..5165)
                     /locus_tag="VN627_04275"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HXD02504.1"
                     /translation="MTLPRPLLGAVLAGGQSRRFGSDKALAVLEGQTLLERAVGRLAG
                     WCDAVVAVGRAEAPVPVVPDWPAPGMGPLGGFAGALRYAQGEGFAAVLTVGVDSLGLP
                     EDLPARLAPGPACVADQPVVALWPVAVLPVLAAILSAPGRHAVRDLIAAAGARSVVLA
                     GASANVNTPEDLARVADGGDLPPAG"
     gene            complement(5162..6055)
                     /gene="nudC"
                     /locus_tag="VN627_04280"
     CDS             complement(5162..6055)
                     /gene="nudC"
                     /locus_tag="VN627_04280"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HXD02505.1"
                     /translation="MKLDIAFAGSPIDRADRLRTDASALDALKGPAARLLLLDGLSPR
                     YGEDGRLAWGSLADADEGSELVFLGLMDGAACFAPVSPGLWGPGRPQGPNFRVLMGGL
                     APDQLATYGGAKALVDWHARHRFCARCGAPTELAKGGWQRNCTAEACKTEHFPRVDPV
                     TIMLVELAGAAEPTVLLGRQPMFPPGNYSALAGFVEPGETIEEAVAREIEEEAGVRAR
                     DVTYIASQPWPFVSNLMIGCHAWADDAALTIDRNELDDARWFTRSEVVRALSGEPESA
                     LKLPPPLAIAHQLLRWWVART"
     gene            complement(6061..7158)
                     /locus_tag="VN627_04285"
     CDS             complement(6061..7158)
                     /locus_tag="VN627_04285"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HXD02506.1"
                     /translation="MTASDDEICRGTAPALAAWYAAHARSLPWRAPPAAAAAGAVPPD
                     PYRVWLSEVMLQQTTVAAVKGYFARFTERWPTVADLAAAEDGEVMAAWAGLGYYARAR
                     NLLACAREVARRGGQFPHTEAGLRELPGLGAYTAAAVAAIAFGQKAVVIDANVERVVA
                     RLFAIGEPLPAGRAAIRAAAEGITPETGAGDFAQAMMDLGATICRAREARCLVCPLAQ
                     LCRARAGGDPTAYPAKAARAARPERAGRAFWIEREGAVWLVRRPGKGMLGGMRALPDD
                     GWSARGDGSGQAPLALDWRAAGVVRHGFTHFTLAMSVSMAAIGAGHPPGEGEWWAIDR
                     IEEAGLPTLFARAAMLARADAAAQTDGRERA"
     gene            7188..7733
                     /locus_tag="VN627_04290"
     CDS             7188..7733
                     /locus_tag="VN627_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HXD02507.1"
                     /translation="MERDRKPAAKGQKTGAARKYERPRGGQARAIADLMPEIGRTAFR
                     RFGFVQSSVVTRWPEIVGPHHAKICAPESIRFAPGEKSGGILQLVVVPAHAPLIQHVI
                     PEIIERVNRFFGYNAVAKVKLRQGVVAQPQAKDRTAPPSLKPIPLELGESLRDIGDPE
                     LRTVLESLARSLDANSRKDRN"
     gene            7735..>8296
                     /locus_tag="VN627_04295"
     CDS             7735..>8296
                     /locus_tag="VN627_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXD02508.1"
                     /translation="MSPKRLLAGLLALIALGPATVAPTIAEAAPAKGGPAVLVIATPQ
                     GSHVVGRPGAANKLVEYVSYTCPHCAAFEKEGAPKLMLTAIRPGRMSVEYRPFMRNIV
                     DVAATLMVGCGAPSRFPGNHAAVLRGQETWFKPPSEDAQQRWRTGDFGTRMRAVAADM
                     NLYPIFTARGYARAELDRCLANEQLAN"
BASE COUNT         1322 a         3146 c         2714 g         1114 t
ORIGIN      
        1 ggccactcag gccatcagcg ccagcaggtc cgaaatcagg caggtcgcaa aacccaccgc
       61 gctatccgaa atcccgtagg gcaccagcag ccgctcgccc accggcatga ccccgcaggt
      121 atagaccacg ttgggcacat agcccgagcg gtcggcatcg acggcggtga ggatcggctc
      181 ctttgtccgc ccgatcaccc gcgaaggatc gtcgcggtca agcagggccg cgcccagcgc
      241 atacttgcgc atcgcgccca ccccgtgggt gatcaccagc cagccggcgt cggtcagcac
      301 cgggctgccg cagttaccga tctggatgaa ttcccaggga tatttcggct caagcagaag
      361 cgagccttcc tcctcccatc gctccagacg gtccgagcgc aggaggtaaa ggttcttgcc
      421 gtcctgccgc cccagcatca tgtactgccc gccgatcttc tgcggaaaca gcgccatgcc
      481 cttgttgcgc ccggccgccc cgaggatcgg ctccagctcg aaccgcttga agtcgaacgt
      541 gcgcagcagt tccgaccgga tcgagctgcc cgaataggcg gtataggtgc cgatccactc
      601 gtaggtcccg tcatcgtggc agaagcgcac gagccggagg tcttccagcc cgccgcgctg
      661 ctgctcggtg atcgggaaga tcaccgtgtt cgagaggctc gaatcctcgt gccggtgcac
      721 gcccaccgcg cagtcgacac aggcgagatc ggcattgtcg agttccaccg ccgtggcaaa
      781 cgagctctgc ggccacaggg tgaagctgcc gttggcctcg gcgatgccct cgcggaaggc
      841 gatcgaggag atgtgaccct cgcccaccgc gcgcaggctc atcacgaacc ggcaggcccc
      901 gttctgcatg ccggtctggt cgggatgcgg cacgatggat gggttcatca gggcggcggc
      961 ggcataggta tattcgtggc agaaataggc cccgatcagc cgcttgcgcg agcgcgagaa
     1021 gcgcgccgga tcgatcctga gcacgtcctc gacctcgcgg tagcgctggt cgaacatggc
     1081 ctcggtctgc cagtgccgct cattgaagtc ggccagcacg cggcgatact ccgcgctcgc
     1141 cgcccgctcc gagagggcgg cgatatcctc caccaaccgg cgcgagcgat cgccctgcgc
     1201 ggtcgtggac tgccacccca ggtgaaaggg gcgcagcacc acgcggctgg gatcggcatg
     1261 cagccgggta tcaaggattt gcaaagggct ggctgacgct tcgcgatccc ggctcaagga
     1321 tatctcccgt tctctctgcc gcggcccgcg cgccgctcca caatgctagc atggaatagt
     1381 gcgccagctg aaacgccagg atcgattccg ccccgcaatt ttcgtttgca ccgcgcgggg
     1441 ttacaccatc gcgtgagcgg ccggtcgcaa tatcggccaa aaccgcgccg cgatcgttgg
     1501 cgccgaagaa ccagcggtgc gcgctcaggg catggtccag ccagcaccgc tcgccggtca
     1561 cgtcccatgc gctgcgcgcc gcctcgatcg ccgcctgggc ttcgaggggc tgctggtcga
     1621 acgggagcga ggcatgggtc aggccgaagg tttccgaacc gatcgggcgg aagtgcccgg
     1681 ccgccgccgt ctggcgcccg gcaatccagc cgagcgtttc cagcccggcg cgggtccagt
     1741 ccgcccggcc gagcagcgcg cccgcctcga tcagcgcctg gctcagccgg ggattgtcat
     1801 agccgagcat ggcctcgaac caggcccagt ccgggcggcg cccggcctcg agcagcgcat
     1861 gaagcaggct gccgccgcgc tcgagcagct cgcgcgcggc gccgtggcct ggatccgcgc
     1921 gcagcatggc cgccgcgcca agcatggcga aagcctgggt gcgcggccag tcgagcccgt
     1981 cgatgaacac cgggcagact tcgtcgaacc acacctgcgc ccagcgccgg aaatcgggat
     2041 cgggcgcgtt ctccaccgtg tggccaaggg cccacagcgc ccggccgttg gaatcgtcgg
     2101 agccggcctc ctcgcaccag gtacgttcga aattcatgaa attgcgaaag cgccgcagtt
     2161 ccgggttcca cgcgtgctgt acgaagctcg cataggtcgt cgaccagcgc atccgttcgc
     2221 tgcgcgaaag gccctgggcc acgttcatca gcatcagcgc gcgggcattg tcatccaggc
     2281 agtagccgtg gcgccggtcg gggaccacgc cgaagccgtg ctggagcatg ccggtcgcat
     2341 cgctcatcgt cagcacgccg gaaaggcccg gcaacacggt cagcggcact tcgcgcgcgg
     2401 gcggcgcggc ggcgcgggcg acaaggcccg ccgtggcatc ggcgaattcg ggccagatcg
     2461 tcgtccgccc ccgcgcatag gcgcgccgct cgatcgcggc gaggcgctcg ggccggtcaa
     2521 gcagggccga aaccgcctcg gcaatcgccg cgctggagcc gggctcgatc agcacgccga
     2581 tctcgtccgc gagcagttcg gtcgcgtgga cataaggcgt ggaaaccacc gccttgccca
     2641 gcgccaccgc gtaggacagc gtgccggagg tcgcctgctg gagattggga tagggcgtca
     2701 catagatgtc gcaggcctcg agctgatcga gcagctcgtc ggtcgcgagg aagcggttgt
     2761 cccaggcgat atggtcttcc acgccgagcc ggcgggccaa ttcggccagg ctgtcgcgat
     2821 agcgttcgcc ctcgtggagc acgcaattgg ggtgagtcgc gccgacgatc cggtagagca
     2881 cctcgggatg gcgcccgacg atcgccggca gggcctcgat cacccgctcc agccccttgc
     2941 ccgggccgag caggccgaac gtcatcagca ccttgcggcc ggccaggccc atttcgcctt
     3001 tgaattgctc cttgcggcca aacggccggt cgggtgcgcc gtgggggatc acctcgacca
     3061 ccgccgcgct ggcgccgtag cggcgcacca gcagttcgcg ggcgtggctg ctcatcacca
     3121 tgatccgcga ggcgcggctg acgagatggc gcagcacccg ctcctgctcg ggcgaaggct
     3181 gggccagcac ggtatggcag gtcaggacca gcggcgcggc gacccggtcg atgaagtcgc
     3241 agaccatcgc cccgtcttcg ccgccgtaga tgccgtattc gtgctggagc cagagcgcat
     3301 cggccccgct ctcattgatc gcccgggcag cgcgcagata ggcttccgca tcctcctgcg
     3361 ggatcacccc tgcaaccccc gaataggtca ccggcgccgt ctcgggattg tcgagcgcat
     3421 agacgtcgag cgcaatctca gggtggtagc gggcaaactg ctcaaccacg tcgcgcgtga
     3481 aagtggcgat gccgcacttg cgcggcgcat aggacccaac cagcgccacc cgccgggcgg
     3541 ggcgcgcgat gtcgcgcggc gacgggacag tcggggcgga aaagatcggt acgatgtccg
     3601 ccgtgccggg ctcgccggag cgcagggggc gaaacgcgtc gctgtgcatg ggcagggtcc
     3661 tcagtggttc gagccggtcg aggaacgcag gccagcgggc cggcaaggcc ggcgctgcac
     3721 ggggatatag gtcccccctg ctcctcgcaa gaacatactc cgcgagtgcc ccccggttcc
     3781 cgcgccgacg ccgccctgcc ccagccgtgc gacgagcatc acaccgttga aatttatcgc
     3841 taatccttca ggaaccagcg ctggcgccag ccatatgtcg caattctggc cgcgccgctg
     3901 gcatccagcg ccgggcggaa gcggaagcgc tccatggcga ggcggcaggt gatcgcatcg
     3961 gcggcagcat cgcggctggc gcgagctacc ttgcaagcgc tgggccggcc atcggttccc
     4021 acggtcagcc agatctcgac atagtccccg agccgcagcg cccggctggc gatcggatag
     4081 tcccgcgccg agttgatgtc cccggcgatc ttggcgagcg gggtgccgcc acccccgccg
     4141 ctgccgctgc ccgatccgcc tgccccggtc ccgctgcccg gtccgcccgc gcccgtcccg
     4201 ctgccaaggg ctgcccccgc ccggtccgca cttcccgtcc ccgccaccgg cggcgcgggc
     4261 ggcttcgcca gcacgatctt cggcaccggc gccgccaccg cccgcggccg cgccttgtcg
     4321 ccggccacac ccgcggcccc ggcagcctcc ggggcggccg cggcgggcgc cggtttcggt
     4381 tcaggctcgg gcgcagtcac cgtcacggta aacgtgctga ccacccgccg cgccacttcg
     4441 gcggtgaacc ccggcgcaaa ggcccgcacc agcagcaccg ccgccgccag atgcagcagc
     4501 cccgccataa ccatggcggc aagccgcacc ggccgcggcg cacgaagatc ggcttcgctc
     4561 atcggccggg tgatgccacg gggcgccggc ggtgcgcaac gggccggcta gccggcggga
     4621 ggcaaatccc ccccatccgc caccctggcc aggtcctcgg gcgtattcac attggcgctc
     4681 gctcccgcca agaccacgct ccgcgcgccc gccgcagcaa tcaggtcgcg caccgcatgg
     4741 cgacccggcg ccgagaggat tgccgccagc accggcagga ccgccaccgg ccacagcgcc
     4801 accaccggct ggtcggccac gcaggccggt cccggcgcca gccgcgccgg caggtcctcc
     4861 ggcaagccaa gcgaatccac gcccacggtc agcaccgccg caaagccttc gccctgcgca
     4921 taacgcagcg ccccggcaaa gccgcccagc ggccccatcc ccggcgccgg ccagtccggc
     4981 acgaccggga ccggtgcctc ggcccggccg accgccacca ccgcatcgca ccaccccgcc
     5041 agccggccta cggcccgctc cagcagcgtc tggccctcca gcacggccag cgccttgtcg
     5101 ctcccgaacc ggcgcgattg cccgcccgcc agcaccgcgc ccagcaaggg gcggggaagc
     5161 gtcaagtccg cgccacccac cagcgcagca gctgatgggc aatcgccaac ggcggcggca
     5221 gcttgagcgc agactcgggc tcgccggaca aggcgcggac aacttcgctg cgggtaaacc
     5281 agcgcgcatc gtccagctcg ttgcggtcga tggtcaaggc cgcgtcgtcg gcccaggcgt
     5341 ggcagccgat catcaggttg gaaacaaagg gccacggctg gctggcgata taggtcacgt
     5401 cgcgcgcccg cacgccggcc tcttcctcga tctcgcgggc cacggcttcc tcgatcgtct
     5461 cgcccggctc gacgaacccg gccagcgccg aatagttgcc gggcggaaac atcggctggc
     5521 ggccgagcag aacggtcggc tcggcggcac cggccagttc gaccagcatg atcgtcaccg
     5581 gatcgacccg cgggaaatgc tcggtcttgc acgcctcggc ggtgcagttg cgctgccagc
     5641 cgcccttggc cagctcggtc ggcgcgccgc agcgggcgca gaagcggtgg cgcgcgtgcc
     5701 agtcgaccag cgccttggcc ccgccatagg tcgccagctg atcgggcgcg agcccgccca
     5761 tcaacacgcg gaagttcggc ccctgcggcc gtcccggccc ccacagcccg ggcgagaccg
     5821 gggcaaagca ggccgcgccg tccatcaggc cgaggaagac cagctccgat ccctcgtccg
     5881 catcggccag tgagccccag gcgagcctgc cgtcctcgcc gtagcgcgga gacaggccgt
     5941 cgagcagcag cagccgcgcc gccggcccct tcagggcgtc gagcgccgag gcatcggtgc
     6001 gcagccggtc ggcccggtca atcggtgacc cggcaaaggc aatgtccagc ttgattggcg
     6061 tcacgcccgt tctctcccat ccgtttgtgc agccgcatcc gcccgggcca gcatggccgc
     6121 ccgggcaaac agcgtcggca gcccggcctc ttcgatccgg tctattgccc accattcccc
     6181 ttcgcccggc ggatggccag ccccaatcgc cgccatgctc accgacatcg ccagcgtgaa
     6241 atgggtgaaa ccgtgccgga ccaccccggc cgcccgccag tccagcgcca gcggcgcctg
     6301 gcccgaaccg tcgccccggg ccgaccagcc atcgtccggc agggcgcgca tcccgccaag
     6361 catgcctttg cccggccggc gcaccagcca gaccgctccc tcacgctcga tccagaacgc
     6421 ccgcccggcc cgctcgggcc gcgccgccct ggccgccttg gccggatagg cggtcggatc
     6481 acccccggcc cgcgcccggc acagctgcgc cagcggacag accaggcacc gcgcctcgcg
     6541 cgccctgcag atcgtggcgc caaggtccat cattgcttgc gcgaaatctc ccgcgccagt
     6601 ctcgggcgtg atcccctcgg ccgccgcccg gatcgccgcc cgcccggcgg gcaggggctc
     6661 gccaatcgcg aacagccgcg ccaccacccg ctcgacattg gcgtcgatca ccacggcctt
     6721 ttgcccgaag gcaatcgccg ccaccgccgc cgccgtatag gcccccagcc ccggcagttc
     6781 gcgcaggccc gcctcggtgt gcggaaactg cccgccccgc cgcgccactt cgcgcgcgca
     6841 ggccagcagg ttacgggcgc gggcatagta gcccagcccc gcccaggcgg ccatgacctc
     6901 gccatcctca gccgcggcca gatcggcaac ggtcggccaa cgctcggtga acctggcgaa
     6961 atagcccttc accgccgcca ccgtggtctg ctgcagcatg acttccgaca gccacacccg
     7021 atagggatcg ggcggcaccg cccccgccgc cgccgccgcc ggcggggcgc gccacggcag
     7081 gctgcgggcg tgggcggcat accaggcagc cagggccggc gccgtgcccc ggcaaatttc
     7141 gtcgtcactg gcggtcacgg cgcggctatg gcatggcagc gcccgcaatg gaacgggatc
     7201 gcaaaccagc cgccaagggg cagaaaaccg gggcagcccg caagtatgag cgcccgcgcg
     7261 gcggccaggc ccgcgccatc gccgatctca tgcccgaaat cgggcgcacc gccttccgcc
     7321 gcttcggctt cgtgcagtcc agcgtggtca cccgctggcc ggaaatcgtc ggcccgcacc
     7381 atgcgaagat ctgcgcgccc gaatcgatcc gcttcgcgcc gggcgaaaag agcgggggca
     7441 tcctccagct ggtcgtagtc ccggcccatg ccccgctgat ccagcatgtc attcccgaga
     7501 tcatcgaacg ggtgaaccgc ttcttcggct acaatgccgt ggccaaggtc aagctgcgcc
     7561 agggcgtcgt cgcgcagccc caggccaagg accgcaccgc cccgcccagc ctcaagccta
     7621 tcccactgga actgggcgaa agcttgcgcg atatcggcga tcccgaactt cgcaccgtgc
     7681 tcgaatcgct cgcccgcagc ctcgatgcca acagccgcaa ggaccgcaac tgacatgtcc
     7741 ccgaagcgcc ttctcgccgg cctgctcgcc ctcatcgcgc tcggcccggc aaccgtggcc
     7801 cccacaattg ccgaagccgc gccggccaag ggcgggccgg ccgtgctggt gatcgccacg
     7861 ccccagggca gccacgtcgt cggccggccg ggcgcggcca acaagctggt cgaatatgtc
     7921 agctacacct gcccgcactg cgccgccttc gagaaggaag gcgcgccgaa gctgatgctc
     7981 accgcgatcc gcccgggccg gatgtcggtg gaataccggc cgttcatgcg caacatcgtc
     8041 gatgtcgcgg ccacgctgat ggtcggctgc ggcgccccct cgcggttccc cggcaatcat
     8101 gccgccgtgc tgcgcggcca ggaaacctgg ttcaagccgc ccagcgaaga cgcccagcag
     8161 cgctggcgga ccggcgattt cggcacccgc atgcgcgccg tcgccgccga catgaacctc
     8221 tatccgatct tcacggcgcg cggctatgcc cgggccgaac tcgatcgctg ccttgcgaac
     8281 gagcagctgg ccaatg
//
LOCUS       DATNBV010000176         2522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1369296,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000176 DATNBV010000000
VERSION     DATNBV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369296"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1314)
                     /locus_tag="VN627_04300"
     CDS             complement(127..1314)
                     /locus_tag="VN627_04300"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXD02509.1"
                     /translation="MTVAAPSVFDAIVLGAGAAGLTCAAQAGQRGRRVVLLDHAEAPG
                     KKILISGGGRCNFTNLHTAPERYLSANPHFARSALSRYTPADFLGLVEAYGIAWHEKT
                     LGQLFCDGSARQIVELLLEECSRGQVDLRCGAAIREVSHADGRFSVRTDAEVVTAPAL
                     VIATGGPSIPKLGATGFAYDLARQFGLKVVEPRPALVPLTLSGEDALFRELSGVATEV
                     AASCGKARFREAALFTHRGLSGPAILQVSSYWRRGDMVGIDFLPGAAEGWLVAAKQAR
                     PKTSVQGALRAALPDRLADALAERLALAGPLAGVTDKALRAAEARLAGWEFRPNGSEG
                     FAKAEVTIGGVSTAELSSKTMEAKKVPGLYVIGEAVDVTGWLGGYNFQWAWASAWAAG
                     QAV"
     gene            complement(1380..>2522)
                     /locus_tag="VN627_04305"
     CDS             complement(1380..>2522)
                     /locus_tag="VN627_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXD02510.1"
                     /translation="WLKTWQIACREDDIPKAGDFHIYRVAHLSWLVVRQEDGSVKAHQ
                     NVCLHRGRPLRDCNAPGATEFRCPYHGWSWKIDGSVKEITAEWDFPGVRESVAQLPGA
                     KVALWNGFVFINPDENAIPIEEYLGPVMIDHFKTFNYPGKYKQAHVRRKMKANWKIVA
                     EAFKESYHVIATHPQMMLTGGDSVQTRYDSFGHFFRAGHGQINSVSPQRGIYRTDEEQ
                     VAEHLAMADLNRNFLRGIIGDEVEQYSDMELNDSTFNDLFPNCHPWGGWIRIVFRFFP
                     GDNPDESIMDVMFLAPWPKDKPKPPPAKTKELAFEQSWAEAPELGSLARIIDQDCGNV
                     PFIQEALKGKKGGKVWTSGYMESMIRAFHDHYDKYMGLDENGEFID"
BASE COUNT          420 a          891 c          781 g          430 t
ORIGIN      
        1 gaatggtcat cactctcagc cggatgcatg atgaaccaac caccgggatg cagcgcgacg
       61 gctttctcag ccagctctcc aaactagaca cgcgctacga ttggcggccg ctggcctagc
      121 gcgttcctaa accgcctgcc ccgccgccca ggcgctggcc caggcccact ggaagttgta
      181 accgcccagc caccccgtca cgtccaccgc ctcgccgatc acatacagcc ccggcacctt
      241 cttcgcctcc atggtcttgg aggaaagctc ggccgtgctg accccgccga tcgtcacctc
      301 ggccttggcg aagccttcgc tgccattggg cctgaattcc caccccgcca gccgcgcctc
      361 ggccgcgcgc agggccttgt cggtcacccc ggcgagcggc cccgcaagcg ccagccgctc
      421 ggccagtgca tcggccagcc ggtcgggcag cgccgcgcgc aaggctccct gcacgctcgt
      481 ctttggccgc gcctgcttgg ccgccaccag ccagccctca gcggcgccag gcaggaaatc
      541 gattcctacc atgtcgcccc gccgccagta cgacgacacc tgcaggatgg ccgggcccga
      601 caggcctcgg tgcgtaaaca gcgccgcctc gcgaaaccgc gccttgccgc agctcgccgc
      661 cacctccgtc gccacccccg acagttcgcg gaacagcgcg tcctccccgc tcagcgtcaa
      721 cggcaccagc gccgggcgcg gctccaccac cttgagcccg aactggcgcg ccagatcgta
      781 ggcgaacccg gtcgcgccaa gtttggggat cgacgggccg ccagtcgcga tgaccagcgc
      841 cggggcggta accacctccg cgtccgtccg caccgagaag cgcccgtcgg catggctcac
      901 ctcccggatc gccgccccgc agcgcaggtc cacctgcccc cgcgaacatt cctccagcag
      961 cagctcgacg atctgccgcg ccgatccgtc gcagaacagc tggccgagcg tcttctcgtg
     1021 ccaggcgatg ccataggcct caaccaggcc gaggaaatcg gccggcgtgt agcggctcag
     1081 ggctgaacgg gcgaagtgtg gattggccga aagataccgc tcgggcgccg tatgcaggtt
     1141 ggtgaaattg caccgcccgc cgccggagat gaggatcttc ttgcccggcg cctcggcatg
     1201 gtcgagcagc accacgcgcc gaccgcgctg cccagcctgg gccgcgcacg tcagcccggc
     1261 ggcccctgcg cccagcacga tcgcatcaaa gacagaaggc gccgccacgg tcatggcgac
     1321 gcctctatca ggttatggcg gcacgggaag gctcccgcac ctgatgccgt agccgaacct
     1381 cagtcgatga actcgccgtt ctcgtcgagg cccatgtact tgtcgtagtg gtcgtggaac
     1441 gcgcggatca tgctttccat gtagcccgac gtccagacct tgccgccctt cttgcccttc
     1501 agggcttcct ggatgaacgg gacgttgccg cagtcctggt cgatgatgcg ggccagcgag
     1561 cccagttccg gcgcttcggc ccacgattgt tcgaaggcca gttccttggt cttggctggc
     1621 ggcggcttgg gcttgtcctt cggccagggc gcgaggaaca tgacgtccat gatgctttcg
     1681 tccggattgt caccggggaa gaaccggaag acgatgcgga tccagccgcc ccagggatgg
     1741 cagttgggga acaggtcgtt gaaggtgctg tcgttcagct ccatgtcgga atactgctcg
     1801 acctcgtccc cgatgatccc gcgcaggaaa ttgcggttga gatccgccat ggcgagatgc
     1861 tcggcgacct gctcttcatc ggtgcggtag atgccgcgct gcgggctgac cgagttgatc
     1921 tggccgtgcc cggcgcggaa aaagtgcccg aacgaatcgt agcgcgtctg caccgaatcg
     1981 ccgccggtga gcatcatctg cgggtgggtg gcgatgacgt ggtaggattc cttgaacgcc
     2041 tcggcgacga tcttccaatt ggccttcatc ttccggcgga cgtgggcctg cttgtacttt
     2101 ccgggatagt tgaaggtctt gaagtgatcg atcatcaccg gcccgagata ttcctcgatc
     2161 gggatggcgt tctcgtcagg gttgatgaag acgaagccgt tccacagcgc caccttggcc
     2221 ccgggcagct gggccacgct ctcacgcacg cccgggaaat cccactccgc ggtgatttcc
     2281 ttgaccgagc cgtcgatctt ccacgaccag ccgtgatagg ggcagcggaa ttcggttgca
     2341 cccggcgcat tgcaatcgcg caaggggcgc ccgcggtgaa ggcagacgtt ctggtgcgcc
     2401 ttgaccgaac cgtcctcctg gcgaaccacc agccagctca ggtgggccac ccgataaatg
     2461 tggaagtcgc ccgccttggg aatgtcgtcc tcgcggcagg cgatctgcca ggtcttgagc
     2521 ca
//
LOCUS       DATNBV010000177         7215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3605592,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000177 DATNBV010000000
VERSION     DATNBV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3605592"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /gene="secY"
                     /locus_tag="VN627_04310"
     CDS             complement(<1..1118)
                     /gene="secY"
                     /locus_tag="VN627_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXD02511.1"
                     /translation="MASRADNIASTLSLANFSKATELKKRIWFTIGALIVFRFLSFVP
                     LPGVNPLILSDLYSRTQGGILDLFNTFSGGGLQRMSLIALGVMPYITASIVIQLAASL
                     HPSLAALKKEGESGRKKLNQYTRYGAVVLTAIQGWFVASGLEAYGASSGLQAVVMPGM
                     VFRVGAVISLIGGTMFLLWLGEQITSRGIGNGTSLIIMAGIVAQMPTFIANLFEGGRS
                     GSISGFTIVGLVVLLVALILGICFFERATRRLLIQYPKRATQKGMMQADRSHLPLKLN
                     TAGVIPPIFASSLLLLPLTITQFAGNSVSPESKTGAFIVTLNQYLAHGKPLYMVLYAL
                     GIVFFSFFYTAVVFNPEETSENLKKNGGFIPGIRPGKNT"
     gene            complement(1285..1806)
                     /gene="rplO"
                     /locus_tag="VN627_04315"
     CDS             complement(1285..1806)
                     /gene="rplO"
                     /locus_tag="VN627_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HXD02512.1"
                     /translation="MKLNDIRDNSGARKGRMRVGRGIGSGKGKTAGRGQKGAKARSGV
                     SVNGFEGGQMPLHMRIPKRGFNNIFAKDFAEVNLGLVQKAIDAGKLDVNATIDHAALK
                     AVGLGRGGKDGVRLLGKGAFSAKATFAVDGVSAGARAAVEAAGGTVTVAEAGPSEQEK
                     KAARAAANKAAKA"
     gene            1947..3437
                     /locus_tag="VN627_04320"
     CDS             1947..3437
                     /locus_tag="VN627_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02513.1"
                     /translation="MAIISTGEPGGRAGLIGRPLAQALILAGLALVLRWSSFGDPDLA
                     ADETFYQTVGIAMHHGALPYVDVWDRKPLGLFLIYWAITGISEAPLAYQLAAWACAAA
                     TAWVIARIARLWTGAQGALLAGGLYLVWLTPLFGFGGQSPVFYNLLVASAALLVLRAR
                     PRLARGGSSRGASMAMLLAGLAITVKTSAAFEGIYFGLALVAASLRSNEPLAWILARA
                     ARWALLGALPTVLIAAWYAGVGHWPEWWHAMVTANLAKGGDWATSSLRLVLLWRLVLP
                     VVLAAAAGLILMRAEDRSFPAGWLLAALVGLAAIPNFYPHYALPLLVVLCLCAAPVLD
                     RGWLGLVAAAILAGLGLHTESPFRPGQAARSARTFAELSAAIRAHDRGPDGSPDLLVY
                     AGPAQLYPMTGSRLPTPLAFETHLSQVTERNVSHLDTVAELRRVLSARPGAVVLPVQV
                     RNGPAIPETWAMVNGYVRANCRPILQRHVEDWLLADDLVVWGDCRR"
     gene            complement(3588..3764)
                     /gene="rpmD"
                     /locus_tag="VN627_04325"
     CDS             complement(3588..3764)
                     /gene="rpmD"
                     /locus_tag="VN627_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HXD02514.1"
                     /translation="MAKIKIKQIGSPIRRPESQKKILVGLGLGKMHRVVEVEDTAEVR
                     GAIAKLPHMVAVVD"
     gene            complement(3770..4510)
                     /gene="rpsE"
                     /locus_tag="VN627_04330"
     CDS             complement(3770..4510)
                     /gene="rpsE"
                     /locus_tag="VN627_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXD02515.1"
                     /translation="MADETNNEGGIAVEAAAPEATGGEQQREGRGPRGGNRGRGGNDR
                     GGRDGNRGRGGNDRNRGRGGDDEGGEELIEKLVHINRVSKTVKGGKRFGFAALVVVGD
                     GKGRVGFGHGKAREVPEAITKATASAKKKMIRVALKEGRTLHHDGRGHFGAGKVNVRS
                     APAGTGIIAGGPMRAVFESLGVADVVTKSVGTSNPYNMIRATFDALSDQTSPKSVAQR
                     RGKKVADLLGRGGASAAEAEAAAEAIAE"
     gene            complement(4513..4863)
                     /gene="rplR"
                     /locus_tag="VN627_04335"
     CDS             complement(4513..4863)
                     /gene="rplR"
                     /locus_tag="VN627_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXD02516.1"
                     /translation="MAKLSLFDRRRRRVRTALKARSGGRPRLSVHRSGRHIYAQIIDD
                     AAGRTVVSASTLTKGSKGPGATTAAAAEVGKALAEAAKAAGVTSVVFDRGGFLFHGRV
                     KALADAAREGGLEF"
     gene            complement(4863..5396)
                     /gene="rplF"
                     /locus_tag="VN627_04340"
     CDS             complement(4863..5396)
                     /gene="rplF"
                     /locus_tag="VN627_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXD02517.1"
                     /translation="MSRIGKVPVAIPGGVTAAVADGVLTVKGPKGTLTLNLRDEISYV
                     VEEGRIVVRPANDTKQARSFWGMQRTLVSNLVTGVTDGYTKVLEITGVGYRANAQGKN
                     LKLQLGYSHDVDFAVPEGIEIKTPDNTTVEISGIDKQKVGQVAAEIRRWRKPEPYKGK
                     GIKYRGEYIFRKEGKKK"
     gene            complement(5396..5791)
                     /gene="rpsH"
                     /locus_tag="VN627_04345"
     CDS             complement(5396..5791)
                     /gene="rpsH"
                     /locus_tag="VN627_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXD02518.1"
                     /translation="MALTDPLGDMLTRIRNGQRAKKDSVLSPASKLRASVLEVLQREG
                     YIRGYSQDATGAHPQLRIELKYFEGEPAIKHVARVSKPGRRVYSGSKELPVVRNGLGI
                     TIVSTPRGVLSDAEARASNVGGEVLAEVF"
     gene            complement(5802..6107)
                     /gene="rpsN"
                     /locus_tag="VN627_04350"
     CDS             complement(5802..6107)
                     /gene="rpsN"
                     /locus_tag="VN627_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HXD02519.1"
                     /translation="MAKLSSVNKNERRKKLVAKYAAKFAALKAQADDKSLDETERLIA
                     RLKLAELPRNANPTRVRNRCTTTGRPRGYYRKFGLCRIELRDLANKGMVPGVTKSSW"
     gene            complement(6135..6716)
                     /gene="rplE"
                     /locus_tag="VN627_04355"
     CDS             complement(6135..6716)
                     /gene="rplE"
                     /locus_tag="VN627_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006960326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXD02520.1"
                     /translation="MAEKYTPRLKAKFEAEIAAAMQAKFGYKNAMEIPKLEKITLNMG
                     VGEASQDKKKVTTAAAEMELIAGQKPVITKAKKSIAQFKLREGMPIGCKVTLRRERMY
                     EFLDRLVTIAMPRIRDFRGLNPKSFDGRGNYAMGLKEQIIFPEISYDQIEKVRGMDII
                     VTTTAKTDEEARELLRLFGFPFPQDAAEQQQAA"
     gene            complement(6709..7026)
                     /gene="rplX"
                     /locus_tag="VN627_04360"
     CDS             complement(6709..7026)
                     /gene="rplX"
                     /locus_tag="VN627_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HXD02521.1"
                     /translation="MAAAKIKKGDSVVVRSGKDKGRTGTVLQVMPKDGKVLVAGVNVI
                     ARHRKPSQVNPQGGIDRVEAPMAISKVSVADKDGKPTRVRFEERDGKKVRVAVKSGET
                     IDG"
     gene            complement(7026..>7215)
                     /locus_tag="VN627_04365"
     CDS             complement(7026..>7215)
                     /locus_tag="VN627_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007394238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HXD02522.1"
                     /translation="RIAVEADDRTVGTTNVIRFDSNAAVLVGKNEEPIGTRIFGPVVR
                     ELRGRGFMKIISLAPEVL"
BASE COUNT         1096 a         2395 c         2390 g         1334 t
ORIGIN      
        1 gtattcttgc ccgggcggat tccgggaatg aacccgccgt tcttcttcag gttctccgag
       61 gtttcctcgg gattgaagac gaccgcggta tagaagaacg agaagaacac gatgccaagg
      121 gcatagagca ccatatagag cggcttgcca tgcgccaggt actggttgag cgtgacaatg
      181 aacgccccgg tcttgctctc gggcgaaacc gagttgccgg cgaactgggt gatcgtcagc
      241 ggcagcagca gcagcgagga ggcgaagatt ggcgggataa cgccggcggt gttgagcttg
      301 agcggcaggt ggctgcgatc cgcctgcatc atgcccttct gcgtcgcgcg cttcggatac
      361 tggatcagca gccggcgcgt ggcgcgctcg aagaaacaga taccgagaat cagcgcgacc
      421 agcaggacga ccaggccgac gatggtgaag ccggagatcg agcccgagcg gccgccttcg
      481 aacaggttgg cgatgaaggt cggcatctgg gcgacgatgc cggccatgat gatcagcgaa
      541 gtgccgttgc cgatgccgcg gctggtgatc tgctcaccca gccacagcag gaacatggtc
      601 ccgccgatca gcgagatcac cgcgccgacg cggaagacca tgcccggcat gaccactgcc
      661 tggaggccgc tggaggcgcc gtaggcctcg agcccggagg cgacgaacca accctggatg
      721 gcggtgagca cgaccgcgcc gtagcgcgtg tactggttga gcttcttgcg cccgctttca
      781 ccttcctttt tcaaggcggc cagcgacgga tgcagcgagg cggccagctg gatcacgatc
      841 gaggcggtga tgtagggcat cacgccgagc gcgatcaggc tcatgcgctg caggccgccg
      901 ccagagaagg tgttgaacag gtcgaggatg ccgccctggg tgcggctgta gagatccgag
      961 aggatcagcg gattcacgcc cggcagcggc acgaaggaaa ggaaccggaa gacgatcagc
     1021 gcgccgatcg tgaaccagat gcgcttcttg agctcggtgg ccttggagaa gttggcgagg
     1081 ctgagcgtgc tcgcgatatt gtcggcgcgt gaagccatgg gatggggtgt gcctcgtgtg
     1141 actgcgggcc cacatagtgc gcccgaaccg tcttgtcgaa cccggccggg ataaatcgcg
     1201 tcgtcccggc ccaacccgga actgccgccc tgcccggatg gggctggcgc agttgggggt
     1261 caggcccggt acgacgttgt cagttcaggc cttggccgcc ttgttggcag cggcgcgggc
     1321 ggccttcttt tcctgctcgc tcggaccggc ttccgccacg gtcaccgtgc cgccggcggc
     1381 ttccaccgcg gcgcgggcgc cggcggaaac gccatcgacc gcgaaggtgg ccttggccga
     1441 gaacgcgccc ttgccgagca ggcggacacc gtccttgccg ccgcggccga ggccgacggc
     1501 cttgagcgca gcgtggtcga tcgtggcatt cacgtcgagc ttgccggcgt cgatcgcctt
     1561 ctggacgagg cccaggttca cctcggcgaa gtccttcgcg aagatgttgt tgaagccgcg
     1621 cttcggaatg cgcatgtgga gcggcatctg gccgccctcg aacccgttca cggaaacgcc
     1681 cgaacgcgcc ttggcgccct tctggccgcg ccctgcggtc ttgcccttgc cggaaccgat
     1741 gccgcgcccg acgcgcatgc ggcccttgcg ggcaccggaa ttgtcacgga tgtcgttgag
     1801 tttcatgtcg tagcactcgc tttcgctttg aatcgcgctt gatgggagcg gggcccttag
     1861 gcgaagcggc accaggagtc accccctttt ccgccccctt ccctcccgga cgatccccgc
     1921 tagactggcc ggaggaaggg gaattcatgg caatcatcag cacaggcgag ccaggcggca
     1981 gggccgggct tatcggcagg ccgctcgctc aggcgctgat ccttgccggg ctggcgctgg
     2041 tgctgcgctg gtcgtcgttc ggcgacccgg acctcgcggc ggacgaaacc ttctaccaga
     2101 ccgtggggat tgccatgcat cacggcgccc tgccctatgt cgacgtgtgg gacaggaagc
     2161 cgctcgggct gttcctgatt tactgggcga tcaccggcat ctcggaggcg ccgctggcct
     2221 accagttggc cgcctgggcc tgcgcggcgg caaccgcgtg ggtcatcgcg cgcattgccc
     2281 ggctgtggac tggtgcgcaa ggggcgctgc tggcgggcgg actctacctc gtctggctga
     2341 cgccgctgtt cggcttcggc gggcagagtc cggtattcta caacctgctg gtcgccagcg
     2401 cggcgctgct ggtcttgcgg gcgcgtcccc gcctcgccag gggcggatca agtcgcggcg
     2461 ccagcatggc catgctgctc gcgggcctgg cgatcacggt caagaccagc gcggcgttcg
     2521 agggcatcta cttcggcctc gcgctggtcg ccgcatcgct gcgctcgaac gagccgctgg
     2581 cctggatcct cgcccgggct gcccgctggg cgctgctcgg cgccttgccc accgtgctga
     2641 tcgccgcctg gtacgcgggg gtggggcact ggccggaatg gtggcacgcc atggtcaccg
     2701 ccaacctcgc caagggtggt gactgggcta cctccagcct gcgcctcgtc ctgctgtggc
     2761 gcctggtgct gccggtcgtg ctggcagcgg ccgccggcct catcctgatg cgcgccgagg
     2821 accggtcctt tcctgccggc tggctgctgg ccgcgctggt gggcctcgcc gcaattccca
     2881 acttctaccc gcactatgcc ctgccgctgc tggtagtcct gtgcctctgc gctgcgccgg
     2941 tgctggaccg cggctggttg ggtctggtgg cggcagcgat cctcgccggt ctcggcctcc
     3001 ataccgaaag cccgttccgt cccggccagg ccgcgcgttc cgcccgaacc ttcgctgagc
     3061 tcagcgcagc cattcgcgct cacgaccgcg gccccgatgg cagcccggac ctactggtct
     3121 acgccggccc agcccaactc tacccgatga caggcagccg gctgcccacc cccctcgcct
     3181 tcgagacaca cctcagccag gtgaccgaac gcaacgtcag ccatctcgac acggtggccg
     3241 aactgcgccg ggtgctgtcc gcgcgcccgg gcgcagtggt tctgccggtg caggtccgca
     3301 acggcccggc gatcccggaa acctgggcca tggtgaacgg ctatgtgcgc gcaaactgcc
     3361 gcccgatcct gcagcgccac gtcgaagact ggctgctggc ggatgacctc gtcgtctggg
     3421 gcgactgccg ccgctgaagc aaatgactgc ttcccagcgc tctcggctcg tatcgagcga
     3481 agtcgagaca ccgcgcgccg ggtccatttg tcgcggcacg tacaccgaac gaaaacggcc
     3541 ccgggtcacc ccgaggccgc ttcgaatttc ccgcctggga accctggtca gtcgaccacg
     3601 gccaccatgt gcggcagctt agcgatggcg ccgcgcactt cggcggtgtc ttccacctcg
     3661 acgacgcggt gcatcttgcc caggcccaga cccacgagga tcttcttctg gctttccggg
     3721 cgacggatcg gcgagccgat ctgcttgatc ttgatcttcg ccatgagact tactccgcga
     3781 tggcttcagc cgcggcttcc gcctcggctg cgctggcgcc gccacggccc agcaggtcgg
     3841 cgaccttctt gccgcgacgc tgggcgaccg acttcggcga agtctggtcg gacagcgcgt
     3901 cgaaggtggc gcggatcatg ttgtaggggt tcgaggtgcc gaccgacttg gtcaccacgt
     3961 cggcaacgcc caggctctcg aagacagcgc gcatcggacc accggcgatg atgcccgtac
     4021 cggccggagc cgaacgcacg ttgaccttgc cggcgccgaa gtggccacgg ccgtcatggt
     4081 gcagggtccg gccttccttg agggcgacgc ggatcatctt cttcttggcc gaggcggtcg
     4141 ccttggtgat ggcttcgggc acttcgcgcg ccttgccatg accgaagccg acccggccct
     4201 tgccgtcgcc cacgacgacc agcgccgcga agccgaagcg cttgccgccc ttcaccgtct
     4261 tggagacgcg gttgatgtgg acgagcttct cgatcagctc ctcgccacct tcatcgtcgc
     4321 cgccacggcc acggttgcgg tcgttgccgc cgcggccacg gtttccatca cggccgccac
     4381 gatcgttgcc gccacggcca cgattaccac cacgggggcc acggccctcg cgctgctgct
     4441 cgccaccggt tgcttccgga gcggcggctt cgacggcgat accgccttcg ttgttcgttt
     4501 cgtcagccat gctcagaact ccagcccgcc ttcacgggcg gcatcggcca gcgccttgac
     4561 gcggccatgg aagaggaagc cgccgcgatc gaacacgacg gaggtcacgc cagcagcctt
     4621 ggcggcttcg gcgagcgcct tgccgacttc ggcggcagcc gccgtcgtcg cgccggggcc
     4681 cttggaaccc ttggtcagcg tcgaggccga aaccacggtg cgaccggcag catcgtcgat
     4741 gatctgcgcg tagatgtgac ggcccgaacg atgcaccgaa agccgcgggc ggccaccgga
     4801 gcgcgccttg agcgcggtgc gaacccgccg gcgacggcgg tcgaagagcg agagcttggc
     4861 cattacttct tcttcccttc cttgcggaag atgtactcgc cgcggtactt gatgcccttg
     4921 cccttgtagg gttcgggctt gcgccaacga cggatctcgg cggcaacctg gcccaccttc
     4981 tgcttgtcga tgcccgagat ctcgaccgtg gtgttgtccg gggtcttgat ctcgatgcct
     5041 tccggaaccg cgaagtcgac gtcatggctg tagccaagct gcaacttcag gttcttgccc
     5101 tgagcgttgg cacgatagcc cacaccggtg atctcaagga ccttggtgta gccgtcggtg
     5161 acgcccgtca ccaggttcga caccagcgtg cgctgcatgc cccagaacga ccgggcctgc
     5221 ttggtgtcgt tggcgggacg gacgacgatg cggccctcct cgacgacata gctgatctcg
     5281 tcgcgcaggt tcagcgtaag ggtgcccttg gggcccttca cggtgagcac gccgtcggcg
     5341 acggcagcgg tcacgccgcc ggggatcgcc accggaactt taccgatgcg gctcatcaga
     5401 acacctccgc cagcacttcg ccgccgacgt tgctggcgcg ggcctcggca tccgagagca
     5461 cgccgcgggg cgtcgagacg atggtgatgc caaggccgtt acggactacg gggagttcct
     5521 tggaacccga gtagacgcgg cggcccggct tggagacgcg agcgacgtgc ttgatcgccg
     5581 gctcgccttc gaaatacttc agttcgatgc gcagctgcgg gtgggcgcca gtggcgtcct
     5641 gcgagtagcc acggatatag ccttcgcgct gcagcacttc gagcacgctg gcgcgcagct
     5701 tcgaagccgg cgaaaggacg gagtccttct tcgcacgctg gccgttgcgg atgcgggtga
     5761 gcatatcacc caggggatcg gtcaatgcca tcgctagatc cttaccagct cgacttcgtc
     5821 acgcccggaa ccatgccctt gttggcaagg tcacggagtt cgatgcggca aaggccgaac
     5881 ttgcggtagt agccgcgcgg gcggccggtg gtggtgcagc ggttgcgtac ccgggtcggg
     5941 ttcgcattgc gcggcagctc tgccagcttc aggcgggcga tcaggcgctc ggtttcgtcg
     6001 agcgacttgt cgtcggcctg ggccttgagc gcggcaaact tcgccgcata ctttgcaacg
     6061 agcttcttac gacgctcgtt cttgttgact gaactcagtt tcgccatgga cttaagctct
     6121 ttctgtcgga acgcttaggc ggcctgctgc tgttcagcag cgtcctgcgg gaaggggaag
     6181 ccgaacaggc gcagcagttc gcgggcctct tcgtcggtct tggcggtggt ggtgacgatg
     6241 atgtccatcc cgcgcacctt ctcgatctgg tcgtagctga tctccgggaa gatgatctgc
     6301 tccttgagac ccatcgcgta attgccgcgc ccgtcgaacg acttcgggtt cagcccgcgg
     6361 aagtcacgga tgcggggcat cgcgatggtg accaggcggt cgaggaattc gtacatgcgt
     6421 tcgcggcgca gtgtgacctt gcagccgatc ggcatgccct cgcgcagctt gaactgcgcg
     6481 atcgacttct tcgccttggt gatcacgggc ttctggccgg cgatgagttc catctcggca
     6541 gcggccgtgg tgaccttctt cttgtcctgg ctcgcctcgc ccacgcccat gttgagcgtg
     6601 atcttctcga gcttggggat ttccatcgcg ttcttgtagc cgaacttggc ctgcatcgcg
     6661 gcagcgattt cagcttcgaa cttggccttc aggcgcggcg tgtacttttc agccatcgat
     6721 cgtctccccg gacttgacgg ccacgcggac cttcttgccg tcgcgctctt cgaagcgcac
     6781 gcgggtgggc ttgccgtcct tgtcagccac agagaccttc gagatcgcca tcggcgcctc
     6841 gacgcggtcg atgccgccct gcggattgac ctggctcggc ttgcgatgac gggcaatcac
     6901 gttgacgccg gcgacgagaa ccttcccgtc cttcggcatt acctgaagga cggtgccggt
     6961 gcggcccttg tccttgcccg aacggacgac gacagagtcg cccttcttga ttttggcggc
     7021 ggccattaca gcacctccgg cgcgaggctg atgatcttca tgaagccacg gccacgcagt
     7081 tcgcgcacga ccgggccgaa gatacgggtg ccaatgggct cttcgttctt gccgacgagc
     7141 acggccgcat tgctgtcgaa gcggatgacg ttcgtcgtcc cgacggttcg gtcatccgct
     7201 tcgacagcaa tgcgg
//
LOCUS       DATNBV010000178         2800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3296675,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000178 DATNBV010000000
VERSION     DATNBV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3296675"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            680..1903
                     /locus_tag="VN627_04370"
     CDS             680..1903
                     /locus_tag="VN627_04370"
                     /inference="COORDINATES: protein motif:HMM:NF008589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HXD02523.1"
                     /translation="MPVARPAGKPARRGWWWRALWIIILLAAAVEFGLWFAGRQSSAP
                     TAATRAQTAAASIQVGVAAVTKGDIPVTLDELGTVTPLATVTVKSQVSGHITDIKFQE
                     GQIVQKGDLLMVIDVRPFTIALESAQATLAHDRALLANARVDLARFQKLMEQDSVAKQ
                     TLDTQRFLVTQDEATVQLDQAAVDNAKLNLDYCHITAPITGRIGLRQVDLGNYVTAGS
                     DALVVIAQIQPITVVFALPEDNLPAVMKQLQAGAVLPTTAYDRSMTTKIATGQLLTLD
                     NQIDTTTGTVKLKAEFDNKDQSLFPNQFVNIRLLVSTLTGVPVVPRAAVLNGAPGDYV
                     YLVQPGDTVAIRPVKLGPADGERVSIKSGLVPGDRVVVDGTDRLRDGAKINIAGSAKP
                     ARGTEDKSKNAATNP"
     gene            2160..>2800
                     /locus_tag="VN627_04375"
     CDS             2160..>2800
                     /locus_tag="VN627_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXD02524.1"
                     /translation="MNPSRPFILRPVATTLLMAAVLLTGIVAYQFLPLSALPEVDYPT
                     IQVQTFYPGASPDVMTSSVTAPLERQLGQMPNLLQMASTSSVGASVITLQFSLDISLD
                     IAEQEVQAAINAAGNLLPSDLPAPPIYAKVNPADAPILTLALTSKTLPLPQIQDLADT
                     RLAQKISQVPGVGLVSISGGQRPAVRVQANLRALSAYGLNIDDLRTTIANANV"
BASE COUNT          482 a          985 c          882 g          451 t
ORIGIN      
        1 tgggcggcga tcatcccatc atttgattcg acccagccgc gaagatgtcg tctgcgcggc
       61 tttcgattca cggaattggc tccccggaac ggaggccaag gcatcccata taaagacgcc
      121 ggctccctga ggagccggcg gatttagtgt tgggtcgggc tgctgggggg acaaccgaca
      181 cctgttgcga tcccggggga gaccgcagac ccggggaaat gctccgcggg cgagcccggc
      241 tacaatcggg gagttcgccg ggctcgatga aggaacttcg cagcccgccg gaagttccaa
      301 tcaggagccg aattttattt tctcctgatc gtacggcagt ccgggggctc gccgcaggag
      361 gggactgtct cggccgtgta atattgcagt gcaacgaagc cgatggcagc ggcagctgcg
      421 ctatcgggtg tcacctcagt ccggcttccc agacagtgcc cattgcaatt tcagcggccc
      481 cagcagacat gaaattttac aaaggactgc cttgccggac atctgccggc gacgggcggg
      541 cgtcagactg cgccgagcgc ctgcccggcg gcgccggaag cggccaagca aagcccgttc
      601 aggaacactg atgcccgacc aggatcctcg gcaagccacc gcctcgacac atgcgcggga
      661 gacccggccc gtgccggaga tgcccgtggc gcgccccgcg ggcaagccgg cccggcgcgg
      721 ctggtggtgg cgcgcgctct ggatcatcat tctcctggcg gcggcggtcg agttcggcct
      781 ctggttcgcc ggccgccaat ccagcgcgcc cacggcggcg acccgcgccc agacggccgc
      841 ggcctcgatc caggtggggg tcgccgctgt gaccaagggc gatatccccg tgaccctcga
      901 cgagctgggc accgtgacgc cgctggcgac cgtcaccgtc aagagccagg tcagcgggca
      961 tatcaccgac atcaaattcc aggagggcca gatcgtccag aagggcgacc tcctcatggt
     1021 gatcgacgtg cggcccttca ccatcgcgct ggaatcggca caggccaccc tggcccatga
     1081 tcgggccctg ctggccaatg cccgggtcga cctcgcccgc ttccagaagc tgatggagca
     1141 ggattccgtc gccaagcaga cgctggacac ccagcgcttc ctggtcaccc aggacgaagc
     1201 gacggtccag ctcgaccagg ccgcggtcga caatgcgaag ctgaacctcg actactgcca
     1261 tatcaccgcg cccatcaccg gccgcatcgg gctgcgccag gtggatctcg gcaattacgt
     1321 cacggccggc agcgacgccc tcgtcgtcat cgcccagatc cagccgatca ccgtcgtctt
     1381 cgctctgccc gaggacaatc tgccggcggt gatgaagcag ctccaggccg gcgccgtcct
     1441 gcccaccacc gcctatgacc gcagcatgac gaccaagatc gccaccggcc agcttctgac
     1501 cttggacaac cagatcgata cgaccaccgg cacggtgaag ctgaaggccg agttcgacaa
     1561 caaggaccag agcctcttcc ccaatcagtt cgtcaatatc cgcctgctgg tctcgaccct
     1621 caccggcgtg ccggtcgtcc cgcgcgcggc ggtcctcaac ggcgcgccgg gcgactatgt
     1681 ctatctcgtg cagcccggcg ataccgtcgc catccgcccg gtcaagctcg ggcccgccga
     1741 tggcgagcgg gtctcgatca agtccggcct cgtccccggg gatcgggtgg tggtggacgg
     1801 caccgaccgc ctgcgcgacg gcgccaagat caacatcgcc ggctcggcga agccggcgcg
     1861 cgggacggaa gataaatcca agaatgcggc gaccaatcca tgaggagcgg gcgcttagat
     1921 ccttcacccc tgcttccttc cccctccccc tgcgggccga taggcccgcg ctttgagggg
     1981 ggagggccgg ggtgggggtg gccgaatcag cagcagaagt ctcgggcttc atgcagcgtc
     2041 gttgtgatcc gcatccgcgg atcgaacccc ccaccctacc ctcccccgca aggggggagg
     2101 gggtgctttc ctgacgcggc ttgcgcggtt gcgggcgtgc aagggcatag gggcgcccca
     2161 tgaacccgtc gcggcccttc atcctgcggc cggtggcgac gaccctgctc atggcggcgg
     2221 tgctgctcac cggcatcgtc gcctatcagt tcctgccgct ctccgctctg ccggaggtcg
     2281 actatcccac catacaggtc cagaccttct atcccggggc gagcccggat gtgatgacct
     2341 cctcggtgac ggcgccgctg gagcgccaac tgggccagat gcccaatctg ctgcagatgg
     2401 cctcgaccag ctccgtcggc gcctcggtca tcactttgca gttcagcctc gacatcagcc
     2461 tcgacatcgc ggagcaggag gtgcaggcgg cgatcaatgc ggcgggcaat cttctgccct
     2521 ccgatcttcc ggcaccgccg atctacgcca aggtcaatcc cgcggacgcc cccatcctca
     2581 ctttggcgct gacctccaag accctgccgc tgccccagat ccaggatctg gccgataccc
     2641 gcctggccca gaagatctcc caggtgccgg gggtggggct ggtcagcatc agcggcgggc
     2701 agcggccggc ggtgcgtgtc caggccaatc tgcgggccct ttccgcctat ggcctcaaca
     2761 tcgacgatct gcgcacgacc atcgccaatg ccaatgtcaa
//
LOCUS       DATNBV010000179         2127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1373753,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000179 DATNBV010000000
VERSION     DATNBV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373753"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VN627_04380"
     CDS             <1..672
                     /locus_tag="VN627_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXD02525.1"
                     /translation="HGESQWSPDGIYDLDRFADDTRAVVRALARPPVLVGASLGGLAS
                     LIVASTPPPPVAGLVLVDIAVRMEPEGTREIGSFMTAAPDGFASIDEAADAVSAYLPH
                     RPRPKDVSGLMRNLRQRADGRWVWHWDPAFITAKPGPKGVGSGNADYLAAAARRLAVP
                     TLLVRGGRSRVVSKEGAAEFVELVPHARLVDVAEADHMVAGDANDRFNEAVFGFLDGL
                     PAQVG"
     gene            799..1971
                     /locus_tag="VN627_04385"
     CDS             799..1971
                     /locus_tag="VN627_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HXD02526.1"
                     /translation="MVWAAVLGADSPIGLTIIRELGERGVKVLAVGRSADAIGRYSRF
                     TARFIATDRPVAEFLPPLVREYRISAVLAFSEGHLLQLAGLKDQLGNCRVLTPDAGQL
                     ATVLDKERTLAIAARLGMRVPVSWAPCKGEDFARRAAELDYPVAVKWADPNGVAQRLR
                     ESGLPLEKVEYAASADELTAILRRYDPIQTWPLVQSWCPGQGLGQMMHMHTGEATLRF
                     QHRRVREWPRSGGISSMCEGVSLDLHREQFALSEKLLREIGWEGPAMVEYRHDAATNT
                     YWLMEVNGRFWGSLPLAHHCGADFAWETWRCGMDLPGTEPRLRTRRARYMAPDLKHLV
                     AVMVDRTMPLSDRLAFAARYLAEFADPRMRYYVWSWRDPLPFLGSVVSAVRNVWHR"
BASE COUNT          328 a          689 c          746 g          364 t
ORIGIN      
        1 catggcgaaa gccaatggtc gcccgacggc atttatgacc ttgaccgctt cgctgatgac
       61 acccgcgccg tggtccgcgc cctggctcgg ccgccggtgc tggtcggcgc gtcgcttggc
      121 ggactggcga gcctgatcgt cgcttccact cccccgccgc ctgtggccgg tctggtgctg
      181 gtcgacattg cagtaaggat ggagcccgag ggcacgcgcg agatcggctc gttcatgacc
      241 gccgcgcccg atggctttgc ctcgatcgat gaggcggccg acgcggtctc cgcctactta
      301 ccgcaccgcc cccggccgaa ggacgtcagc ggactgatgc gcaaccttcg gcagcgggcc
      361 gacggtcgct gggtgtggca ctgggacccg gccttcatca ccgccaagcc aggcccgaaa
      421 ggggtcggct ccggcaatgc cgactatctc gccgctgccg cgcggcggct ggccgtgccc
      481 accctgctgg tgcggggtgg gcgcagccgg gttgtgagca aggaaggcgc ggcggagttc
      541 gtcgaattag tcccccacgc ccgactcgtc gacgtggccg aagccgacca catggtggcc
      601 ggcgatgcca acgaccggtt caatgaggcc gtgttcggct tcctggatgg actgccggcc
      661 caggtaggct gaggccgacc cccgtcatcc cggcgctagt gcggccttat cggcgaaggc
      721 gcggtgctcc gccgtggtgt taaccgccaa gttcgcctag taggctgtgg atcatgcggt
      781 gaggggcatt cgcaagcaat ggtgtgggct gcagttctgg gggcagacag tccgatcggg
      841 ctgaccatca tccgcgaact gggggagcgt ggcgtcaagg tcctggcggt cggccgatcg
      901 gccgacgcga tcgggcgata ctcgcgcttc acggcccgct tcatcgcgac cgatcgcccg
      961 gtggcggagt tcttgccgcc gctggttcgc gaataccgga tctctgccgt gctggccttt
     1021 tccgaaggcc acctgcttca actggcgggc ctcaaggacc agttgggcaa ctgccgcgtc
     1081 ctcacgccgg acgcaggcca gcttgcgacg gtgctagaca aggagcgcac gctggccatc
     1141 gccgcccgcc tgggcatgcg cgttccggtg agctgggccc cgtgcaaggg tgaggacttc
     1201 gcgcggcgtg ccgcagagct tgactatccg gttgcggtga aatgggcaga cccgaacggc
     1261 gtggcgcagc gcctcaggga aagcggcctg ccgctcgaaa aggtcgaata tgccgcctcg
     1321 gctgacgagc tgaccgccat tctgcgccgc tacgatccga tccagacctg gccactggtg
     1381 cagagctggt gtcccggcca gggtcttggg cagatgatgc acatgcacac aggcgaggcc
     1441 accttgcggt tccagcaccg gcgagtgcgc gaatggcccc gttccggggg gatatccagc
     1501 atgtgcgagg gggtaagcct cgatctacac cgggagcagt ttgccctttc ggagaagttg
     1561 ctccgtgaga tcggctggga gggcccggcc atggttgagt atcgccacga cgccgccacg
     1621 aacacctact ggctcatgga ggtgaacgga cgtttctggg ggagcctgcc gctggcgcac
     1681 cattgcgggg ctgatttcgc ctgggaaacc tggcggtgcg ggatggacct gcccggcacc
     1741 gagccgcgcc tgcgcacgcg ccgtgcgcgc tacatggcgc ccgacctgaa gcatctggtc
     1801 gccgtgatgg tggaccgaac gatgcccctg tcggaccgcc tggcattcgc cgccagatac
     1861 cttgccgaat tcgccgatcc gcggatgcgc tattacgtct ggtcgtggcg cgatccgcta
     1921 ccgttcctgg gcagcgtggt ctccgcggtc aggaatgtct ggcaccgctg aacgtgcggc
     1981 acagccccgc cacggaacgt tcgatccgcg caaggcacac gcgggtatag gcatcgctga
     2041 gccgataggg gtcatggagg tgcggaacgg ggaattcggc ataggtgccg agcagccccc
     2101 tcggcagatc ggcaaagcgt ggctggg
//
LOCUS       DATNBV010000180         3579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4251696,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000180 DATNBV010000000
VERSION     DATNBV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4251696"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VN627_04390"
     CDS             complement(<1..215)
                     /locus_tag="VN627_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD02527.1"
                     /translation="MDFNYSEMQQMLVDSATKLVADSYTLEDLREQKHNPAGYNAKNW
                     AQFAEMGWLALPLPEDAGGLGCSIEDAV"
     gene            complement(245..1450)
                     /locus_tag="VN627_04395"
     CDS             complement(245..1450)
                     /locus_tag="VN627_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD02528.1"
                     /translation="MHFEFDSSLDAFRQEIREFLAENLTEGMRLRQQYEGTLFAGSKE
                     DEREWIRLLAKKNCEVYAWPKELGGQDWSAMKQFVFEDELYKGFAPPISFNVLHMIGP
                     VIYRFGSEDLKARFLPGLRSGEIQFCQGFSEPGSGSDLASLRTRADRVGDKYVINGSK
                     IWTSGAHEANHCFCLVKTDTTVKPQRGISMILIPMDTPGIQVRTIPQINGDEEHLCQV
                     FFENVEVPAGNLIGEENMGWTYAKKLLEGERTSSSYIFWNKREMRRLVNMAGQEEQNG
                     NPIAKDLVWRSRLAKIQAELIALEWSVLRVLANEEFAKGLPAAASALKVRGSQLQQAI
                     TEAQTDLLGEKAMRRYSVHAMPGDQGGMWPEHVLGRTNTGLLLRAATVFGGSLQVQRN
                     ILAATAFGL"
     gene            1751..2674
                     /locus_tag="VN627_04400"
     CDS             1751..2674
                     /locus_tag="VN627_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD02529.1"
                     /translation="MIDPSKLVLRADSLDGDRILVTGGGSGLGRIMAEGFAWLGARVY
                     ICGRRGGLLDEAAADINGAVGCQAVRGLPCDIRSARSITELADAIWADGGALTGLVNN
                     AAANFISRSEDISERGFDAIADTVFRGTWLMTQEVARRWLNAGDQGAVLSILTTWVWS
                     GGPFAVPSAMSKAAIDVMTQSLAVEWGGRGIRLNALCPGAFPTKSVEDRLLVREQGMH
                     NAPGNPLRRNGTPDELANVAAFLLSDGAGFINGQTIAVDGAGWQMGEATFAGLTAWTD
                     ADWQEAREKIRANDAGDKAARTVDAPARGRR"
     gene            2729..>3579
                     /locus_tag="VN627_04405"
     CDS             2729..>3579
                     /locus_tag="VN627_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXD02530.1"
                     /translation="MTEGLFTAAERHPDKAAAILAPSGDRLTFGELVDRSARLAAILR
                     QHLDEGDRVAILMENRLDYYVAVWAARRSGLRYVPINWHLHARESAYIVNDCGARALI
                     ASEHLRALAEQSAAQSPNLELLLSVDGDFGAFRAIAGMPPAPPAPEVEGSAMMYSSGT
                     TGAPKGIIRPLGGHPAGTPGPAEDLITRGYGFNAETVFLCPGPLYHAAPIHWTAGTQV
                     MGGTSVVFPSFDPEAVLAAIERHRVTHALFVPTHFVRLLKLPEEVRARYDLSSLKAVI
                     HAAAPCPP"
BASE COUNT          588 a         1188 c         1162 g          641 t
ORIGIN      
        1 accgcatcct cgatcgagca gccgaggccc ccggcgtctt ccggtagcgg cagggcgagc
       61 cagcccattt cggcgaactg ggcccagttc ttcgcattgt agccggccgg attgtgcttc
      121 tgttcgcgca ggtcctcgag cgtgtagctg tcggcaacga gcttggtggc gctgtccacc
      181 agcatctgct gcatctcgga atagttgaaa tccatggctg aaatccgtca gaattgcgtc
      241 ggcgtcagag gccgaaggcg gttgccgcga ggatgttgcg ctggacctgg aggctgccgc
      301 cgaacacggt ggccgcgcgc aggagcagcc cggtattggt gcggcccagc acgtgttccg
      361 gccacattcc gccctggtcg ccgggcatgg cgtggaccga atagcggcgc atcgccttct
      421 cgccgagaag atcggtctgc gcctcggtga tcgcctgctg cagctgcgaa ccgcgaacct
      481 tgagcgcaga cgccgctgcg ggaaggccct tggcgaattc ctcgttggcg aggacgcgca
      541 gtaccgacca ctcgagcgcg atcagttcgg cctggatctt ggcgaggcgg ctgcgccaca
      601 ccaggtcctt ggcgatcggg ttgccgttct gctcctcctg gccggccatg ttgaccaggc
      661 ggcgcatctc gcgcttgttc cagaagatgt acgagctgct cgtgcgctcc ccttcgagca
      721 gcttcttggc gtaggtccac cccatgttct cttcgccgat caggttgccg gcgggaacct
      781 cgacgttctc gaagaacacc tggcacaggt gttcctcgtc accgttgatc tggggaatgg
      841 tgcggacctg gatgcccgga gtgtccatcg ggatcaggat catgctgatg ccgcgctgcg
      901 gcttcaccgt ggtatcggtc ttgaccaggc agaagcagtg gttggcctcg tgtgccccgc
      961 tggtccagat cttggaaccg ttgatgacgt acttgtcgcc cacccggtcg gcgcgggtgc
     1021 gcagcgaagc caggtccgat ccggagcccg gctccgaaaa gccctgacag aactggatct
     1081 cgcccgaacg caggccgggc aggaaacggg ccttgagatc ctcgctcccg aatcggtaga
     1141 taaccggccc gatcatgtgc agcacgttga agctgatcgg gggtgcaaag cccttgtaca
     1201 gttcatcctc gaagacgaac tgcttcatcg cgctccagtc ctgcccgccg agttcctttg
     1261 gccaggcgta aacctcacag ttcttcttcg ccagcagcct gatccattcg cgctcgtcct
     1321 ccttcgaacc ggcgaacagc gtaccttcgt attgctggcg caggcgcatt ccctcggtca
     1381 ggttctcggc gaggaattcc ctgatttcct gacggaacgc atcaagggaa gaatcgaact
     1441 cgaaatgcat ctgtcgtctc caaacagtcc cgctcgcggg gtcggacgtg atcagcgctc
     1501 gccgaagggg gtctgcgtcc agacccgatt gcaccgccgg cgcagcatcg caaccacatc
     1561 cgcgattcat cgcgatatgg atggcacaag ccgcagggag aaagctgcgc cgccatgcac
     1621 cgatacaaca ggcgtccgaa ggggcacgaa ccgcgcgcgg acatgatatg cgcgcggcgg
     1681 ccggctcgcc ggcttgcgcg ggcacccaag ttcggtttgt atcacgcacc acgccaggag
     1741 gggcaggccg atgatcgatc cgagcaagct cgtcctgcgt gctgattccc tcgacgggga
     1801 ccgcattctc gtcactggcg gcggctcggg gctgggcagg atcatggccg aaggcttcgc
     1861 ctggcttggc gcgcgcgttt atatctgcgg ccggcgcgga ggtttgctgg atgaggctgc
     1921 agccgacatc aatggggcgg tggggtgcca ggccgtgcgc gggctgccct gcgacatccg
     1981 ctccgccagg tccattaccg agctggccga tgccatctgg gccgatggcg gtgcgctgac
     2041 cgggctggtc aacaatgcgg cggcaaactt catctcccgt tcggaagata tctcggagcg
     2101 cggtttcgac gcgatcgcgg ataccgtgtt ccgcggcacc tggctgatga cccaggaagt
     2161 agcccggcgc tggctaaacg cgggtgacca gggcgcggtc ctttcgatcc ttacaacgtg
     2221 ggtgtggagc ggcgggcctt ttgcggtacc ttccgcaatg tcgaaggcgg cgatcgacgt
     2281 gatgacccaa tcgctcgccg tggaatgggg cggtcgcggt atccggctca acgccttgtg
     2341 tccgggcgcc ttcccgacca agtcggtgga agaccgcctg ctcgtcaggg aacaagggat
     2401 gcacaatgcc ccgggcaacc cgctgcgccg caacggcacg cccgacgaac tggccaatgt
     2461 cgcagccttt cttctttccg acggtgccgg attcatcaac gggcagacca tcgcggtcga
     2521 cggcgccggc tggcagatgg gcgaggcgac cttcgccggg ctgaccgcgt ggaccgatgc
     2581 ggactggcag gaggcgcggg agaaaatcag ggccaacgac gccggcgaca aggcggcgcg
     2641 gaccgtggat gctcccgccc gcggccggcg ctgaaagcac accggccgcg aggtgcggac
     2701 aagagaaagc aagggagaat tgccgataat gaccgaaggg ctgtttaccg ccgccgagcg
     2761 ccaccccgac aaggccgcgg ccatcctggc gccgtcgggc gataggctca cctttggtga
     2821 gctggttgat cgctcggccc ggctcgccgc catccttcgc cagcacctcg atgagggtga
     2881 ccgtgtcgcg atccttatgg agaaccggct cgactactat gtcgccgtct gggccgcccg
     2941 gcgctctggc ctgcgctatg tcccgatcaa ctggcacctc catgcgcgcg agagcgccta
     3001 tatcgtcaac gactgcggcg cccgcgccct gatcgccagt gagcatctgc gcgccctcgc
     3061 cgaacagtcg gcggcgcaaa gccctaacct ggagctgctc ctgtcggtcg atggcgattt
     3121 cggtgccttc cgcgcaatcg ccggcatgcc cccggccccg ccagcgccgg aggtggaagg
     3181 gtcggcgatg atgtattcct ccggcacaac cggcgcgccg aaggggatca tccggcccct
     3241 cgggggccac cccgccggca cgcccggacc tgccgaggac ctcatcacgc ggggctatgg
     3301 cttcaacgcg gaaacggtct tcctctgccc cggcccgctc taccacgccg cgccgattca
     3361 ctggaccgcc ggcacccagg tgatgggcgg cacgtccgtc gtctttccca gcttcgatcc
     3421 cgaagccgtg ctcgccgcga tcgagcggca ccgggtgacc catgccctgt tcgtgccgac
     3481 ccatttcgtc cgccttctga aattgccgga ggaggttcgc gcgcgctacg acctctcgag
     3541 cctgaaggcc gtgatccatg ccgctgcccc ctgccctcc
//
LOCUS       DATNBV010000181         7187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3298673,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000181 DATNBV010000000
VERSION     DATNBV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3298673"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="VN627_04410"
     CDS             <1..120
                     /locus_tag="VN627_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HXD02531.1"
                     /translation="LNLSEIAGVLEVSIPRVHQLKAQALEQLRKALSGVADIL"
     gene            351..1049
                     /locus_tag="VN627_04415"
     CDS             351..1049
                     /locus_tag="VN627_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXD02532.1"
                     /translation="MSSCDTCVVRNRAICAALDSTEIGALNTIGRTRTLAPGESLIWE
                     GDDSVLVANVIDGVLKLSTGTEDGREQIVGMVFPSDFIGRPFGKTTGHGVTALTEARV
                     CVFNRRDFDSFAREHPALEHKLLQRTLTELDRTRRWMLLLGRKSASEKVASFLLEMCE
                     RASGSGCESGEEPAEVLLDLPVSRQQIADVLGLTIETVSRQFTRLKAEGVIDLPSRRE
                     VHIRDRFVLAAEAG"
     gene            complement(1113..1799)
                     /locus_tag="VN627_04420"
     CDS             complement(1113..1799)
                     /locus_tag="VN627_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HXD02533.1"
                     /translation="MKKTMLSAAVAVAALAMPAAAHAGSNEGKLQIKLLATGVLPDGD
                     ISRVNNATAIAGTPLAANPHTYASDTVVPTVAIEYFFNPNVSVETICCLTKHHVNGAG
                     SLAGTNLVNDVTVIPATVTVKYHAPLGPIKPYVGVGPTLFIMVDSKPGATAATFSSST
                     KMSSEVGVAVQAGVDVPLGNSGYALSLDAKKYWVSTTARWFNGAGTEVLSTKHKLDPW
                     VLSAGVAYRF"
     gene            2031..3713
                     /gene="ccoN"
                     /locus_tag="VN627_04425"
     CDS             2031..3713
                     /gene="ccoN"
                     /locus_tag="VN627_04425"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HXD02534.1"
                     /translation="MVTLTGRAGIWLAALLIAILAVAAAADTGFAIHMGIVAAACLVG
                     LWFTLTKADYSALGRGLSAAPDQSRYDDDPIRWGVIATVFWGMAGFAAGLFIALQLAF
                     PVLNLGFEYTTFGRLRPLHTSAVIFAFGGNALIATSFYVVQRTCRARLAFPGLARFVF
                     WGYQLFIVLAATGYLLGITQGKEYAEPEWYVDLWLTIVWVSYLAVFVGTLVKRTEPHI
                     YVANWFYLSFILTVAMLHLVNNVNLPVSWVGSRSYPLWAGVQGALVQWWYGHNAVGFF
                     LTAGFLAMMYYFVPKQAERPIYSYRLSIIHFWALIFLYIWAGPHHLHYTALPDWAQTL
                     GMVFSVMLWMPSWGGMINGLMTLNGAWDKVRTDPIIRMFVISLAFYGMSTFEGPMMSV
                     KSVNSLSHYTDWTIGHVHSGALGWNGMITFACIYYLTPRLWARTRMYSLRMINWHFWL
                     ATVGIVFYAASMWVAGITQGLMWREYGADGYLVNSFAETVAALHPMYLLRAFGGLLYL
                     SGAVIMTVNVWKTVAGSPLREEAPLRDAAYDAEKDRPLIAGSTAAAYAAAAE"
     gene            3727..4515
                     /gene="ccoO"
                     /locus_tag="VN627_04430"
     CDS             3727..4515
                     /gene="ccoO"
                     /locus_tag="VN627_04430"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HXD02535.1"
                     /translation="MTTASKNEPGSKGFLASHKKLERNVTLLAVLAFAAVTVGGIVEI
                     APLFWIDNTIEKVEGMRPYTPLEQAGRDIYVREGCYLCHSQMIRPFRDETERYGHYSL
                     AAESMYDHPFQWGSKRTGPDLARVGGRYSDEWHVQHLTDPRSVVPESVMPMYAFLKDK
                     DLSASDVEGSMSKELTALYRVGVPYSKEQIAHADEDMKAQAGMGDATELAKRWPKAQA
                     RDFDGDPNRLTEMDALVAYLQVLGTMVDVNSAAAQEDLAKETGR"
     gene            4512..4673
                     /locus_tag="VN627_04435"
     CDS             4512..4673
                     /locus_tag="VN627_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HXD02536.1"
                     /translation="MTQYDTLRHLADSWGLVAMGAVFLALCLWPFRPGARASNDAAAR
                     MIFEDEDNG"
     gene            4666..5613
                     /gene="ccoP"
                     /locus_tag="VN627_04440"
     CDS             4666..5613
                     /gene="ccoP"
                     /locus_tag="VN627_04440"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HXD02537.1"
                     /translation="MADHKKIDEATGVETVGHEWDGIEELNNPLPRWWVLTFYACILF
                     AIAYVVIYPAIPGRHKASDGLWKWSSRGALAAEVAAADKARGPVMAALAATPIEALPA
                     NPDLMEKAVAGGKAAFRVNCVQCHGAGAAGSKGYPNLNDDDWLWGGDLKTIETTITHG
                     IRFAGDDQTRQSQMPSFGHDGILSPEQVGDVATYVRSLSGEEKRSAATQRGAAIFAAN
                     CAVCHGPEGKGGRQVGAPNLADHIWLYGGNQADVVASVTNAHAGVMPAWGHRLDPVTI
                     KMLAAYVHSLGGGEKFAPAPAVAAAVAAPAAAPAGAAAK"
     gene            5675..7150
                     /gene="ccoG"
                     /locus_tag="VN627_04445"
     CDS             5675..7150
                     /gene="ccoG"
                     /locus_tag="VN627_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HXD02538.1"
                     /translation="MRNPAPVATGLYEKRKAVFPRQIDGKFRRLKWAIMAVTLTIYYA
                     TPWLRWNRGPYAPDQAVLVDLAHRRFYMFSIEIWPQEFYFVAGLLIMAGIGLFLVTSA
                     VGRAWCGYACPQTVWTDLFQHVDRLIDGDRNAQMRLAGAPWGPRKVFKRGLKWTIYLA
                     ISFWTGGAWIMYFADAPTLTVDFWSGHAAPVAYATIAVLTGITFWFGGFMREQVCIYM
                     CPWPRIQTAMLDEKSLLVTYKDWRGEPRASVKATKADPDGKFGDCIDCNQCVAVCPTG
                     IDIRQGPQIGCITCALCIDACDRVMQQVGRPRGLIDYATLEDAAREQAGNPPTPHRKL
                     IWHPRTLVYLGIWSAIGLALLFALGTRRHLDLAVQKDRNPPYMLLSDGSVRNAWTLKL
                     KNMEGRPRNMEVALEGLPGAVMWTDTMGRDQAARRLVEQVPADTTEPVRVYVAAPAGT
                     GARTGRGELAFVMTALDKEGGRDRVETRFDAPDEEEHHDDR"
BASE COUNT         1168 a         2361 c         2403 g         1255 t
ORIGIN      
        1 ttgaaccttt cggaaatcgc cggggtgctg gaagtctcga tcccgcgggt gcaccagctc
       61 aaggcccagg cgctggagca gttgcgcaag gccctgagcg gcgtggcgga catcctgtga
      121 caggaatcgc ccgggctcga cccggggccg gtgtcggatc ttcaaggggc cttgctagat
      181 ggctgcggca cagcttcgca ccggatctcg gccccggatc gagcccggga cgacgtgggc
      241 cgccgcgcgc ttgatctccg gcaatgacat cagccaaatg tctaaacctt atgtatgttt
      301 aaagctgctc acgattgccc cggaacccgt ccggtggtaa gcggttgatc atgtcctctt
      361 gcgatacctg cgttgtccgg aaccgcgcca tctgcgcggc cctcgattcc accgagatcg
      421 gcgcgctcaa taccatcggt cgcacgcgaa ccctggcacc gggcgaatcg ctgatctggg
      481 aaggggacga ttccgttctc gtcgccaacg tgatcgatgg cgtcctgaag ctctccactg
      541 gcacggaaga tgggcgcgag cagatcgtcg gcatggtgtt cccgtcggat ttcatcggcc
      601 ggcccttcgg caagaccacc gggcatggcg tgaccgcgct gaccgaggcc agggtttgcg
      661 tgttcaaccg ccgcgatttc gacagtttcg cgcgcgaaca tcccgcgctg gaacacaagc
      721 tgctccagcg cacgctgacc gaactggatc gcacgcgccg ctggatgctg ctgctggggc
      781 gcaagtcggc cagcgagaag gtggccagct tcctgctcga gatgtgcgag cgggcgagcg
      841 gttcgggctg cgagagcggg gaggagcccg ccgaagtgtt gctcgatctc ccggtttcgc
      901 gccagcagat cgccgacgtg ctggggctga cgatcgagac ggtcagccgg cagttcaccc
      961 ggctcaaggc cgaaggggtg atcgacctgc cttcgcgccg cgaggtccat atccgcgacc
     1021 gcttcgtcct cgccgcggaa gcggggtgaa gaaatcccct cccgcaggcg ggaggggaga
     1081 ggagtggcgg cgggctgcgc cgccggggct ggtcagaagc ggtaggccac gcctgcgctc
     1141 agcacccacg ggtcgagctt gtgcttggtg gagagcactt cggtgcccgc cccgttgaac
     1201 cagcgcgccg tggtcgacac ccagtacttc ttggcatcga ggctcagcgc atagccgctg
     1261 ttgcccagcg gcacgtccac gcccgcctga accgcaacgc cgacctcgct cgacatcttg
     1321 gtggaggagg agaatgtggc cgcggtcgcg ccgggcttgc tgtcgaccat gatgaacagg
     1381 gtcgggccca cgccgacgta gggtttgatg ggtccgagcg gcgcgtggta cttgaccgtg
     1441 accgtggccg ggatgaccgt gacgtcattg accaggttgg tgccggcaag cgagcctgcg
     1501 ccgttgacgt ggtgcttggt caggcagcag atcgtctcga ctgagacgtt cgggttgaag
     1561 aaatactcga tcgccaccgt cggtacgacc gtgtcgctgg cataggtgtg cgggttggcg
     1621 gcgagcggcg tgccggcaat ggcggtcgcg ttgttgaccc gcgagatgtc gccgtcgggc
     1681 agcacgccgg tggcgagcag cttgatctgg agcttgccct cattgctgcc ggcgtgggcc
     1741 gcggcgggca tggcgagcgc ggcgacggcc accgcggcgg aaagcatggt cttcttcata
     1801 tccctcatcc tgtgccgccg ctcgcctgcc cctcgcaggc gtttcatgcg gcgtcgcaca
     1861 taagggtggg gcgaaatggc gggcgctcat tgatcgagcg caatcgcgcg caatttacga
     1921 ctttggtcga atatgtgtgc gtccaatgca tgttttgatc cgggtcaatg ctgcattgca
     1981 ggggcagagg catgggtgag cccacatctc cggcctgaga gggaaggctc atggttacct
     2041 tgactgggcg cgctggcatc tggctggctg cgctgcttat tgccatcctg gcggtcgccg
     2101 ctgccgcgga caccggcttt gccattcaca tgggcattgt cgccgcggcc tgcctcgtcg
     2161 gcctctggtt caccctgaca aaggccgact attcggccct ggggcgcggg ctctccgccg
     2221 cgccggacca gagccgctac gatgacgatc cgatccgctg gggcgtgatc gccacggtgt
     2281 tctggggcat ggcgggcttt gccgcggggc tgttcatcgc cctgcagctg gccttcccgg
     2341 tcctcaacct cggcttcgaa tacaccacct tcgggcggct gcggccgctg cacacttctg
     2401 ccgtcatctt cgccttcgga ggcaatgcgc tgatcgcgac ctcgttctac gtggtccagc
     2461 gcacctgccg ggcgaggctg gccttccccg gcctcgcccg tttcgtgttc tggggctacc
     2521 agctgttcat cgtgctggcg gcgaccggct acctgctcgg gattacccag ggcaaggaat
     2581 atgccgagcc cgaatggtac gtggacctgt ggctgacgat cgtgtgggtt tcctaccttg
     2641 ccgtcttcgt cggcacgctg gtcaagcgga ccgagccgca catctacgtg gccaactggt
     2701 tctacctctc gttcatcctg accgtggcca tgctccacct ggtcaacaac gtgaacctgc
     2761 cggtttcgtg ggtgggcagc cgctcgtacc cgctgtgggc gggcgtacag ggcgcgctgg
     2821 tgcagtggtg gtatgggcac aacgcggtcg gcttcttcct gaccgccggc ttcctcgcca
     2881 tgatgtacta cttcgtgccc aagcaagccg agcggccgat ctattcctac cggctctcga
     2941 tcatccactt ctgggcgctg atcttcctct acatctgggc tggcccgcac catctccact
     3001 acaccgcgct gcccgactgg gcgcagacgc tgggcatggt cttctcggtc atgctgtgga
     3061 tgccgagctg gggcgggatg atcaacggcc tgatgacgct gaacggcgcc tgggacaagg
     3121 tccgcaccga tccgatcatc cgcatgttcg tgatcagcct cgccttctac gggatgagca
     3181 cattcgaggg cccgatgatg agcgtgaaga gcgtgaactc gctctcgcac tacaccgact
     3241 ggaccatcgg ccacgtccat tccggcgcgc tggggtggaa cgggatgatc accttcgcct
     3301 gcatctacta cctgacgccg cgcctctggg cccggacccg gatgtattcg ctgcggatga
     3361 tcaactggca cttctggctc gcgaccgtgg ggatcgtgtt ctacgcggct tcgatgtggg
     3421 tggcggggat cacgcagggc ctgatgtggc gcgaatacgg cgccgacggc tacctggtga
     3481 actccttcgc cgaaacggtc gccgcgctgc acccgatgta cctgctgcgc gcctttggcg
     3541 gcctgctcta cctctcgggc gcggtgatca tgaccgtgaa cgtgtggaag accgtggccg
     3601 gctcgccgct tcgcgaggaa gcgccgctgc gcgacgccgc ctacgacgcc gagaaggacc
     3661 gtccgctgat cgccggcagc accgctgctg cctatgccgc tgccgctgag tgagggggcg
     3721 cgagacatga ctaccgcatc gaaaaatgaa ccgggttcca agggctttct ggcgagccac
     3781 aagaagctcg agcgcaacgt gaccctgctt gccgtcctcg ccttcgcggc ggtgacggtg
     3841 gggggcatcg tcgagatcgc gccgctgttc tggatcgaca acacgatcga gaaggtggag
     3901 ggcatgcgcc cctacacccc gctggaacag gccgggcgcg acatctacgt gcgcgagggg
     3961 tgctacctct gccacagcca gatgatccgt ccattccgcg acgagaccga gcgctacggc
     4021 cactacagcc tcgccgcgga atcgatgtac gaccatccgt tccagtgggg ctccaagcgc
     4081 accgggcccg atctcgcccg cgtgggcggg cgctattcgg atgaatggca tgtccagcat
     4141 ctgaccgatc cgcgctcggt cgtgccggaa tcggtgatgc cgatgtatgc cttcctcaag
     4201 gacaaggacc tctcggcgag cgacgtcgaa ggctcgatga gcaaggaact gaccgcgctt
     4261 taccgtgtgg gcgtgcccta ttcgaaggag cagatcgctc acgccgacga ggacatgaag
     4321 gcgcaggccg gcatgggcga tgcgactgag ctggccaagc gctggcccaa ggcccaggcc
     4381 cgcgatttcg acggcgatcc caaccgcctg accgagatgg atgcgctggt cgcctatctc
     4441 caggtgcttg gcaccatggt cgacgtcaac agcgccgccg cccaggagga tctcgccaag
     4501 gagacgggtc ggtgacgcaa tatgacaccc tgcgccatct ggcggattcc tgggggctcg
     4561 tcgccatggg cgcggtgttc cttgccctgt gcctctggcc cttccgcccc ggcgcgcgcg
     4621 cgagcaacga cgccgccgcg aggatgatct tcgaggatga agacaatggc tgaccacaag
     4681 aaaatcgatg aagccaccgg cgtcgagacc gtcggacacg agtgggacgg catcgaggaa
     4741 ctgaacaacc cgctgccacg ctggtgggtg ctgactttct acgcgtgcat cctgttcgcg
     4801 atcgcttacg tggtgatcta tccggcgatc ccggggcggc acaaggcgag cgacgggttg
     4861 tggaagtggt cgagccgcgg ggcgctcgcc gccgaggtgg ccgctgcgga caaggcccgc
     4921 ggcccggtta tggcggcgct agctgcgacc ccgatcgagg cgctgccggc caatcccgac
     4981 ctcatggaaa aggccgtggc gggcggcaag gccgccttcc gcgtcaactg cgtccagtgt
     5041 cacggtgccg gcgcggcggg aagcaagggc taccccaacc tcaacgatga cgactggctg
     5101 tggggcggcg atctcaagac cattgagacc accatcaccc acggcatccg cttcgccggt
     5161 gacgaccaga cccgccagag ccagatgccg agcttcgggc acgacgggat cctgtcgccc
     5221 gagcaggtcg gcgacgtggc cacctatgtc cgctcgctct cgggcgagga gaagcgcagc
     5281 gccgccaccc agcggggcgc cgcgatcttc gcggccaact gcgcagtctg ccacgggccc
     5341 gagggcaagg gcgggcgcca ggtcggcgcg ccgaacctgg ccgatcacat ctggctctac
     5401 ggcggcaacc aggccgatgt ggtcgccagc gtgaccaacg cccatgccgg cgtgatgccc
     5461 gcctggggcc accgcctcga tccggtgacc atcaagatgc tggccgccta tgtccactcg
     5521 ctgggcggcg gcgagaagtt cgctccggcg cctgccgtgg ctgcagccgt cgctgctccg
     5581 gcagcggcac cggccggagc ggccgccaag tgaaataagg tcggcaggcc cgccgctgag
     5641 gggaggcggg cctgccacaa caaggtgcaa gatcatgcgt aacccagccc cggtcgccac
     5701 tgggctctat gaaaagcgca aggccgtctt tccccgccag atcgatggca agttccgccg
     5761 gttgaaatgg gcgatcatgg ccgtcaccct gacgatctac tatgcaacgc cgtggctgcg
     5821 ctggaatcgc ggaccctatg cccccgacca ggcggtgctg gtcgatctcg cccaccgccg
     5881 cttctacatg ttctcgatcg agatctggcc gcaggagttc tacttcgtcg cgggcctgct
     5941 gatcatggcg ggcatcggcc tgttcctggt gaccagcgcc gtgggccggg catggtgcgg
     6001 ctatgcctgc ccccagacgg tgtggaccga cctgttccag cacgttgacc gcttgatcga
     6061 cggcgatcgc aatgcccaga tgcggctggc cggcgcgcca tgggggccgc ggaaggtgtt
     6121 caagcgcggc ctcaagtgga cgatctacct cgccatctcg ttctggaccg gcggcgcctg
     6181 gatcatgtac tttgccgacg cgccgacgct caccgtcgat ttctggtccg gccacgccgc
     6241 gccagtggcc tacgcgacca tcgccgtgct gaccggcatc actttctggt tcggcggctt
     6301 catgcgcgaa caggtgtgca tctacatgtg cccgtggccg cgcatccaga ccgccatgct
     6361 cgacgagaag agcctgctcg tcacctacaa ggactggcgc ggcgagccgc gcgccagcgt
     6421 caaggcaacc aaggccgacc ccgatggcaa gtttggcgac tgcattgact gcaaccagtg
     6481 cgttgccgtc tgcccaacgg ggatcgacat ccgccagggc ccgcagatcg gctgcatcac
     6541 ctgcgccctg tgcatcgatg cctgcgaccg ggtcatgcag caggtcggcc gcccgcgcgg
     6601 cctgatcgac tatgccacgc tggaagatgc ggcacgcgag caggcgggca atccacccac
     6661 cccgcaccgc aagctgatct ggcatccgcg cacactggtc tacctcggca tctggagcgc
     6721 gatcggcctt gccctgctgt tcgcgctcgg cacccggcgg catctcgacc tcgccgtgca
     6781 gaaggaccgc aacccgccct acatgctcct gagcgatgga tcggtgcgca atgcctggac
     6841 cctgaagctg aagaacatgg aaggccgccc gcgcaacatg gaagtggcgc tcgaaggcct
     6901 gccgggcgcg gtgatgtgga ccgataccat gggccgcgat caggccgcgc gtcggctggt
     6961 cgaacaggtg ccggccgata ccaccgagcc ggtgcgggtc tatgtggccg cgccggcggg
     7021 gacgggcgcc aggacgggta ggggcgaact ggccttcgtc atgaccgcgc tggacaagga
     7081 aggtggccgc gaccgggtcg aaacccggtt cgatgcgccc gacgaagagg agcaccacga
     7141 tgaccgctga gcacacgggc ccggccccgc gccccttcac cgggcgg
//
LOCUS       DATNBV010000182         2253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4588509,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000182 DATNBV010000000
VERSION     DATNBV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4588509"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VN627_04450"
     CDS             <1..510
                     /locus_tag="VN627_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidase"
                     /protein_id="HXD02539.1"
                     /translation="GWYSTYWVKMLNDIEVLDRPDDNYWMKVAYTIPDTPRADVKPGQ
                     TGIKTVPINRMVPRSFMTNIKPGASLPAGAPALLRGIAFGGDTGVGRVDLSSDGGTTW
                     RPAELGRDEGKYGFRRWESSFTFPAKGPYTLMVRCTNSDGLAQPDRPNWNPSGFMRNV
                     IEATPVVAG"
     gene            523..849
                     /locus_tag="VN627_04455"
     CDS             523..849
                     /locus_tag="VN627_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXD02540.1"
                     /translation="MSSRLPLLLLAAIVLAPVPGHSEPPLTLTSVTVELPESDNMFTG
                     PGSEALNNNCLACHSAGMVLNQPALPKATWQAVVAKMIHIYKAPVEEKDVGPIVDYLA
                     GIKPAN"
     gene            complement(869..2149)
                     /locus_tag="VN627_04460"
     CDS             complement(869..2149)
                     /locus_tag="VN627_04460"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXD02541.1"
                     /translation="MGNSTVDLTFERALAAEIAASELMRMRVVAVTLAILLVADQLIF
                     LFARGLIEQFVQKPLPGWLPLRVIGPFLVYETVALVILRYRLARERSFPTIARYANAV
                     IETSLPTVILWWVTQFASPEIAFGAWPAMLYFVFIVASTLRLNFLLPAFTGAVAAAGY
                     LGLVLWVLSRHDINTDPLNYLTKAAIMVFAGIVAGLVALRLRAKFRQVFMEAASRERI
                     TNLFGQHVSPAVVDRLLDRPAEFAGEVSEVCVMFLDIRNFTANARGRPPEEVVEFLNA
                     AFAFMIESIDRHGGFINKFLGDGFMAIFGAPIEDRAAASHATAAAREILAEIDRRGLP
                     EAPWPLSIGIGLHIGPAVTGNVGSPRRKEYTAIGDTVNLASRLEQLTKEYSARLIVSD
                     AVMGALSGTAGTATSLGAVAVRGYAEPVGIWRLD"
BASE COUNT          428 a          729 c          745 g          351 t
ORIGIN      
        1 ggttggtatt cgacctattg ggtcaagatg ctgaacgaca tcgaggtgct ggatcgcccc
       61 gacgacaact actggatgaa ggtggcctat accattcccg acacgcctcg cgccgatgtg
      121 aaacccgggc agacgggcat caagacggtg ccgatcaacc ggatggtgcc ccgttccttc
      181 atgaccaaca tcaagcccgg cgccagcctg cctgccggcg ccccggcgct tctgcggggc
      241 atcgccttcg gcggcgacac cggcgtcggg cgggtggatc tctcgagcga tggcggcacg
      301 acctggcggc ccgcagagct cggccgcgac gaaggtaaat atggattccg gcgctgggaa
      361 agctccttca ccttccccgc caaggggccc tataccctga tggtccgctg cacgaacagc
      421 gatggcttgg cgcagcccga tcggcccaat tggaatccga gcggcttcat gcgcaatgtc
      481 atcgaggcga cgcccgtcgt cgccggctga aggaggccga gcatgtcatc caggttgccc
      541 ttgctgctgc tggcggccat cgtcctggcg ccggtgccgg gccatagcga gccgccgctc
      601 acgctgacct cggtcaccgt cgagcttccg gagagcgaca acatgttcac cggccccgga
      661 tcggaggccc tcaacaacaa ttgcctcgcc tgtcattcgg ccggcatggt gctcaaccag
      721 cctgccttgc cgaaagccac ctggcaggcg gtggtcgcca agatgatcca tatctacaag
      781 gcgccggtgg aagagaagga cgtgggcccg atcgtcgatt atctggcggg catcaaaccc
      841 gccaattgaa gtgccgtgga attcagcatc aatccagccg ccagatgccc accggctcgg
      901 catagcctct gaccgcgacg gcgccgagcg aggtcgcggt gccggccgtg ccgctcagcg
      961 cccccatcac cgcatcggag acgatcagcc gggcggaata ctccttggtg agctgctcga
     1021 gccgcgaggc cagattcacg gtgtcgccga tggcggtgta ttctttgcgc cgcggcgagc
     1081 cgacattgcc ggtgaccgcc ggaccgatat gcaagccgat gccgatgctg agcggccagg
     1141 gcgcctcggg caggccgcgg cggtcgatct cggccaggat ctcccgcgcc gcggcggtgg
     1201 cgtgactcgc ggcggcacgg tcttcgatcg gcgccccgaa gatcgccatg aagccgtcgc
     1261 ccaggaattt gttgatgaag cccccatgcc ggtcgatcga ttcgatcatg aaggcgaagg
     1321 cggcattgag gaattcgacg acctcctccg ggggccggcc acgcgcattg gccgtgaaat
     1381 tgcggatgtc gaggaacatc acgcagacct cgctgacctc gccggcgaat tccgccgggc
     1441 ggtccagcag ccgatccacc accgcgggag agacatgctg gccgaagaga ttggtgatgc
     1501 gctcgcgcga ggccgcctcc atgaagacct gccggaactt cgcgcgcagc cgcagggcga
     1561 cgaggccggc gacgatcccg gcgaagacca tgatcgcggc cttggtgaga tagttgagcg
     1621 ggtcggtgtt gatatcgtgg cgcgacagga cccagaggac cagccccaga tagcctgcgg
     1681 cggcgaccgc gccggtgaag gcgggaagca ggaagttgag ccgcaacgtc gaggcgacga
     1741 tgaagacgaa atagagcatc gccggccagg cgccgaaagc gatctccgga cttgcgaatt
     1801 gcgtgaccca ccacaggatg accgtcggca ggctggtctc gatgaccgca ttggcatatc
     1861 tggcgatcgt cgggaagctg cgctctcgcg caaggcggta acgcaagatg accagcgcca
     1921 ccgtctcata gacgaggaac ggcccgatca cccgcagggg cagccagccc ggcagcggct
     1981 tctgcacgaa ctgctcgatc agcccgcggg cgaacaggaa gatcagctga tcggcgacga
     2041 gcaagatggc caaggtgacg gccaccacac gcatgcgcat cagctcgctg gcggctattt
     2101 ccgccgccag cgcgcgttcg aaggtaaggt cgacggttga attccccatg cgcgccgagc
     2161 ttaacccagg caaggacgtt ctcgaaggcg aagtctgcaa ggtggcaagg cctcggggtc
     2221 ggcgcgggtt attccgccgc ttgcaatgcc cac
//
LOCUS       DATNBV010000183         4235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_754811,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000183 DATNBV010000000
VERSION     DATNBV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754811"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VN627_04465"
     CDS             <1..238
                     /locus_tag="VN627_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXD02542.1"
                     /translation="TGWLTVRGKARDHYSRTYDAPMPYMLRLTDDGVALHASMVREGR
                     ATHGCIGLPYAFAARLYDAMQVGGPVLIVGTNPA"
     gene            307..837
                     /locus_tag="VN627_04470"
     CDS             307..837
                     /locus_tag="VN627_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HXD02543.1"
                     /translation="MAARRPSSRALRRTAVLGVALALLTGTVGPERSFRDRLLTAHNA
                     ERAAVGVGPLAWNAHLAASAQDWADHLAATGQFRHAPETIADPQGENLWAGTRGYFSL
                     EDMVGAWGREKRFFRQGTFPANSTTGRVEDVGHYTQLVWRDTSDVGCAEARGAGEDVL
                     VCRYASPGNYRGERPF"
     gene            1009..1224
                     /locus_tag="VN627_04475"
     CDS             1009..1224
                     /locus_tag="VN627_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02544.1"
                     /translation="MTILIAFWGVPLLTMLVVALACLVSGRTRRAAARFLGLDVHTPS
                     SPQPGHRPVPLRIDARTDDHHDGRSAA"
     gene            1375..2961
                     /locus_tag="VN627_04480"
     CDS             1375..2961
                     /locus_tag="VN627_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXD02545.1"
                     /translation="MTRQPEMTFDALVVGAGLCGSWVAKELTSGGMTVGLVDAGALLP
                     DSLFTPVRSGAGLFSLRRHLFRLKLLATGRTDLGFSDVASEQTHRLFLDRRHHPYATS
                     GADFTWHRLRAVGGRGHVWGRVMLRMTERQLAGWPVSYAELAPYYEEVERLLELGGDR
                     STDEAVPDGAYVHHRSLNALEQAFCAAVERGKPERRAVVNHVAGYDPAALSPMLRIAQ
                     ATGQLTLLPETVATRLLIDDRAGPDDRITGIGAACARTGAQRELRARHVILAASAFET
                     LRLLLNSRSDGHPEGLGNAHGLLGTGLLEHLNATVFEQLPASSRNPRPTYRHNPFKLN
                     EEPHGFYIPPLRPAAPATAPAPAERFGIQGTISADTGIFYIGAFGETDRSQANRLTLD
                     PARRDKFGVPLAAIHFAWSDNDLATWRCMNETLADMTEAFCASSGVKFGHSVVGALRK
                     AIQQNAVPVAGTNHECGGAQMGTDPETSVVDAHNRLWEAPNVLVCDAACFPTLPPQNP
                     TATTMALAVRASRRLLADAR"
     gene            2958..4079
                     /locus_tag="VN627_04485"
     CDS             2958..4079
                     /locus_tag="VN627_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD02546.1"
                     /translation="MTASVPSDPFPAGARVLVTGATGFIGGHLVERLAALGAAEIRCP
                     VRDRRGAARLTGGPVRAEPADLADADRMASLVAGTDYLFHCAWDIADPQANIPILRSL
                     LAACTAEAPRRFVLTSSFSVYQPFPDGTLTEATADGDRENAYVDVKLTLEDMVLAATR
                     AGRLNGTVIQPAIVYGPRGGGWTRLAAERLRTGEVVLPDDGAGLCNPVHVDDVVDAML
                     LAAVRPGAVGERFIAAGPEAVTWAEYHGRLAHEIGAPPPVLVPAATILAALAAAEAAP
                     APSAPRRLVRRLLRSGRVRRLTGLLPLLRRLIAPLGRWAGLPERERHWPERPILELYR
                     GKATASWRKAEQAIGFRPRVSFDEGMRQTGAWLRGEAGA"
BASE COUNT          588 a         1631 c         1358 g          658 t
ORIGIN      
        1 caccggctgg ttgaccgtac gcggcaaggc gcgcgaccac tattcgcgca cctacgacgc
       61 gccgatgcca tacatgctgc gcctcactga cgacggcgtg gcgctgcacg ccagcatggt
      121 gcgcgaggga cgggcgaccc atggctgcat cggcctgccc tatgccttcg ccgcccggct
      181 ctatgacgcg atgcaggtgg gcggcccggt gctgatcgtg ggaaccaatc cggcgtgact
      241 ccgctcgtcg catcgcggcc gggccattaa cctatagtta ggcttgtccg cgtcacatgg
      301 gcgccgatgg ctgcccgtcg tccttcctcc cgcgccctgc gccgcaccgc cgtgctcggc
      361 gtggccctcg ccctgctcac cgggacggtc ggccccgagc gttcgttccg cgaccgcctg
      421 ctgaccgcgc acaatgccga gcgcgccgcg gtcggcgtcg ggccgctggc ctggaatgcc
      481 cacctcgccg cctctgcgca ggactgggcc gatcacctcg ccgccaccgg ccagttccgc
      541 cacgcccccg agaccatcgc cgatccccag ggcgaaaatc tctgggccgg cacgcgcggc
      601 tacttttcgc tggaagacat ggttggcgcc tggggccgcg agaagcggtt cttccgccag
      661 ggcaccttcc ccgccaacag cacgaccggc cgggtcgagg acgtgggtca ctacacccag
      721 ctggtctggc gcgacaccag cgacgtgggc tgtgccgagg cgcggggcgc cggtgaggac
      781 gtactcgtct gccgctatgc gagccccggc aactatcgcg gcgaacgccc cttctgagcc
      841 cttccggggt tctccccaga ccctgacgcc gcctgcgccg agccgcgcgg gtcgtccaaa
      901 tgtggcacat cgccgtttat ctgcgcttgc ctcgtgaaca caatcgcgcc tcccgtgttt
      961 accccttgtt aattaacagc ttgtcgggga aacaccgtgg cctggatagt gacgatcctg
     1021 atcgcattct ggggagttcc gctgctgacg atgctggtgg tggcccttgc ctgtctggtc
     1081 tccggacgca cgcggcgtgc ggccgcacgt ttcctcggcc tcgacgttca cacgccatcc
     1141 tcgccgcagc cggggcacag gcccgttccc ttgcgtatcg atgctcgcac cgacgatcac
     1201 cacgacggac gatccgccgc ctgatcaccc ggttgccgcg ccccgcccag cgggaacttt
     1261 ccacctgaac cgcggttggg gcgtcaggcg agtggagaag ttcatgcgga tcgggggccc
     1321 agtgtcgcgt cgctcgccag cgggcctcgc cccggtcatg cctccgcccc gaccttgacc
     1381 cgccagcccg aaatgacctt cgatgcgctc gtcgtcggcg ccggactgtg cggttcctgg
     1441 gtagccaagg aactgaccag cggcggcatg acggtcggcc tggtcgacgc gggcgcattg
     1501 ctccccgaca gcctgttcac ccccgtgagg tccggggccg gtctgttcag cctgcgccgc
     1561 cacctgtttc gcctcaagct gctcgccacc ggacgcaccg accttggctt cagcgacgtg
     1621 gcgagcgaac agacccaccg gctgttcctc gaccgccggc accaccccta tgccacgtcc
     1681 ggcgccgact tcacctggca ccgcctgcgc gcggtgggcg ggcgcggcca tgtctggggc
     1741 cgggtcatgc tgcggatgac cgagcggcaa ctggcgggct ggccagtaag ctatgccgaa
     1801 cttgccccct attacgaaga ggtcgagcgc ctgctggaac tcggcggcga ccgttcgacc
     1861 gacgaggccg tgccggacgg cgcctatgtg caccaccgct cgctcaacgc gctcgagcaa
     1921 gccttctgcg ccgccgtgga gcgcggaaag ccggagcgcc gcgccgtcgt caatcacgtt
     1981 gccggctacg atccggcggc cctctcgcca atgctgcgga ttgcgcaggc gaccgggcag
     2041 ctcacgctcc tgcccgaaac cgtggccacc cgtctcctga tcgatgacag ggcggggccc
     2101 gatgaccgga ttaccggaat cggcgctgcc tgcgcccgca ccggcgccca gcgcgagctt
     2161 cgcgcccgcc acgtgatcct cgccgcctcg gcattcgaaa ccctacggct gctgctcaat
     2221 tcccgcagcg atggccatcc cgagggcctc ggcaatgccc atggcctgct cggcactggc
     2281 ttgttggagc atctcaacgc gacggtcttc gaacagctcc ccgcctcgtc tcgcaatccg
     2341 cgccccactt accggcacaa cccgttcaag ctgaacgagg agccccacgg cttctacatc
     2401 ccaccgctcc ggcccgccgc cccggcaacc gctcccgccc cggccgaacg cttcggcatt
     2461 cagggcacga tctcggccga cacgggcata ttctatatcg gcgccttcgg cgaaaccgac
     2521 cgttcccagg caaatcgcct gacgctcgat ccggcgcggc gcgacaagtt cggcgtgccc
     2581 ctggccgcca tccacttcgc ctggtcggac aacgacctcg ccacctggcg ctgcatgaac
     2641 gagaccctcg ccgacatgac cgaggcgttc tgcgcttcgt ccggggtgaa gttcggccac
     2701 tcggtcgtcg gtgcgctgcg caaggcgatc cagcaaaacg cggtgccggt ggccggaacc
     2761 aaccacgaat gcggcggcgc gcagatgggc accgacccgg aaaccagcgt ggtcgatgcc
     2821 cacaatcgcc tgtgggaagc gcccaacgtg ctggtgtgcg atgccgcctg ctttcccacc
     2881 ctgccgccgc agaacccgac agcgacgacc atggcgctgg ccgtccgggc cagccgccgg
     2941 ctcctggcgg atgcccggtg acggcgtccg tgccctccga tcccttcccc gccggcgcgc
     3001 gggtgctggt caccggggca accggcttca tcggcggcca cttggtcgag cggctggccg
     3061 ccctcggcgc cgccgaaatc cgctgcccgg tgcgcgaccg gcgcggggcc gcgcggctta
     3121 ccggcggacc cgtccgcgcc gaaccggccg acctcgccga cgcggaccgc atggccagcc
     3181 tcgtcgccgg caccgattac ctgttccact gcgcctggga catcgccgat ccgcaggcca
     3241 acatcccgat cctgcgcagc ctgctggcgg cctgcacagc agaggcgccg cgccgcttcg
     3301 tgctcaccag ttcgttctcg gtctaccagc ccttccccga cggcaccctc accgaagcca
     3361 ctgccgatgg cgatcgcgag aatgcctatg tcgacgtcaa actgacgctc gaggacatgg
     3421 tcctcgccgc cacccgcgcc gggcgcctca atggcacggt gatccagccc gcgatcgtct
     3481 atggcccgcg cggcggcggg tggacgcggc tcgcggccga gcggcttcgg accggggagg
     3541 tggtcctgcc cgacgatggc gcaggcctgt gcaatccggt ccatgtcgac gacgtggtcg
     3601 atgccatgct gctcgccgcg gttcgccccg gagccgtggg cgagcggttc atcgccgccg
     3661 ggcccgaggc ggttacctgg gcggaatacc acggccggct ggcccacgag atcggcgccc
     3721 cgccgccggt gctcgtgccc gccgcaacaa tccttgcggc ccttgccgcc gcggaagcag
     3781 cccctgcccc ctctgccccc aggcgactgg tccgccgcct gctgcgatct ggccgggtgc
     3841 gccgcctgac cgggctgctg cccctgctgc gccgcctcat cgccccgctc ggtcgctggg
     3901 ccgggctgcc ggagcgcgaa cggcactggc ctgagcggcc gatcctcgag ctctaccggg
     3961 gcaaggcgac ggcgagctgg cgcaaggccg agcaggcaat cggcttccgc ccgcgcgtgt
     4021 cgttcgacga aggcatgcgc cagaccggcg cctggctgcg cggcgaggcg ggcgcatgac
     4081 tgccaccgcc gaaccccccg ccgcagccgc accgcgcccc catggccgga tcggggatat
     4141 ccccatcctc cacggctggc gcgccatctc gatcctgctg gtgctctcct cccactggct
     4201 gccgctgccc aaggtcctgg cgctaaacga tgccg
//
LOCUS       DATNBV010000184         2153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3300786,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000184 DATNBV010000000
VERSION     DATNBV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3300786"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VN627_04490"
     CDS             <1..736
                     /locus_tag="VN627_04490"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXD02547.1"
                     /translation="IASATPPGKDKLFWYNDYLAGRLKDSKVSLHLGSAAGLDDVTAL
                     DPAVAILAAGTRRIDLPIIGADHAIVRDAFDLLMGERPLELAAGSHVLVYGGGETGCE
                     TAEFCAEHGMKVTLVSRSPANKLARSADAVYRGGLIRRLHANPLVTIVDNTHVLSVGD
                     RGVVIETNGSDGAETRTVVVDRLYLAQGRQPDNRLADALERAGIAVSVIGDSRKVGRI
                     GDAVHTAYQAVLGLKAQYVPPVPAAC"
     gene            complement(750..1409)
                     /locus_tag="VN627_04495"
     CDS             complement(750..1409)
                     /locus_tag="VN627_04495"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXD02548.1"
                     /translation="MREQAEKRRRMVETQLIARGIADGRVLEAMGQVPREVFVDEALR
                     QFAYDDTALPIAAEQTISQPYIVALMAEAAAIGPGDLVLEVGAGSGYAAAVLGAMGAE
                     VIAIERHSELVALARERMLLLGYPNVGVEEGDGSIGLPALAPFEAIVVSAASREVPPP
                     LLEQLAPGGRLVIPVGPPDAQSLLRITRGTDGTNRREDLGPVRFVPLIGAHALPPGSP
                     G"
     gene            1631..>2153
                     /locus_tag="VN627_04500"
     CDS             1631..>2153
                     /locus_tag="VN627_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HXD02549.1"
                     /translation="MSWQWAALATLVPIALLFAFAALIEHGGARAAARPHAHRRLRHL
                     AYTLALGVYCSSWTFYGAVGSVVRDGWNYLPIYLAPALVLLLAPRFLGRLSRAVAEEK
                     AKTVSDFIAARFGHDVVVARLVTVIALLGTIPYVALQLRSIGNAVSLVSGTPVGLPAT
                     IAAAAVLALFAILF"
BASE COUNT          307 a          796 c          705 g          345 t
ORIGIN      
        1 catcgcctcg gccaccccgc cgggcaagga caagctgttc tggtataacg actacctcgc
       61 cggccgcctg aaggacagca aggtttcgct ccacctcggc tcggccgccg ggctggatga
      121 cgtcaccgcc cttgatccgg cggtcgcgat ccttgccgcc ggaacgcggc ggatcgacct
      181 gccgatcatc ggcgccgacc acgcgatcgt gcgcgacgct ttcgacctcc tgatgggcga
      241 acgcccgctc gaactggcgg ccggcagcca tgtccttgtc tacggtggcg gcgaaaccgg
      301 ctgcgagacg gccgagttct gcgccgaaca cggcatgaag gtcaccctcg tcagccgctc
      361 gccggccaac aagctcgccc gctccgccga tgcggtctac cgcggcggcc tgatccgccg
      421 gctccacgcc aatccgctgg tcaccatcgt cgacaatacc cacgtgctca gcgttggcga
      481 cagaggcgtg gtgatcgaga ccaacggctc cgacggtgcc gagacccgca cggtggtggt
      541 ggatcgcctc tatctcgccc agggccgcca gcccgacaac cggctggccg atgcgctgga
      601 gcgcgccggg atcgcggttt cagtcatcgg cgacagccgc aaggtcggcc gcatcgggga
      661 tgccgtgcac accgcttacc aggccgtgct cgggctcaag gcgcaatacg tcccgccagt
      721 gccggccgcc tgctgaagct ggccgctcgt cagcccgggc tgcccggcgg cagggcatgg
      781 gccccgatca gcggcacgaa gcgcactgga ccaaggtcct cacggcggtt cgtgccatcc
      841 gtgccgcggg ttatccgcag cagtgactgc gcgtctggcg ggcccaccgg gatgaccaga
      901 cgcccacccg gggccagttg ctccagcagg ggcggcggga cctcccggct ggcggccgaa
      961 acgacgatgg cctcgaacgg ggcgagggcg ggcaggccga tcgaaccgtc gccctcctcc
     1021 accccgacat tcggatagcc cagcagcagc attcgctcgc gggccagcgc caccagttcg
     1081 gaatggcgct cgatggcgat gacctcggca cccatcgcgc cgagcacggc cgcggcataa
     1141 ccggagcccg cgcccacctc cagcaccagg tcgccgggtc cgatcgcggc ggcctcggcc
     1201 atcagcgcga cgatataggg ctgcgagatc gtctgctctg ccgcgatggg cagcgcggtg
     1261 tcgtcatagg cgaattgccg cagtgcctcg tcaacgaaga cctcgcgggg cacctggccc
     1321 atcgcttcga gcacgcgccc gtcggcaatc ccccgggcga tcagctgcgt ctcgaccatc
     1381 cgccggcgct tttcggcctg ttcacgcatg gccggcatcc tcctccggcc tggccgaacc
     1441 gggcacccgc tcggctcccc ggccgtaatc tgccaggacg ccaccgcagt cgaggaacag
     1501 ttgcattgcc cttcgcctct ctggggctga acggcgggaa atgcgattgg gtccggtcac
     1561 gcccaccgtt gacctcaccg caagcgcagt cgattgtgca ggccatgggt cagaggaagg
     1621 ggcaggcgcg atgagctggc aatgggcggc acttgccacg ctcgtgccga tcgcgctgtt
     1681 gttcgccttt gccgccctca ttgagcatgg cggcgcacgc gccgcggccc ggccccatgc
     1741 tcaccggcgc ctgcgacacc tcgcctatac tctcgcgctc ggcgtctact gctcgagttg
     1801 gacgttctac ggcgcggtcg gctcggtcgt gcgcgatggg tggaactacc tgccgatcta
     1861 ccttgctcct gccctggtcc tcctgctcgc cccgcgcttc ctcggccggc tctcgcgggc
     1921 cgtggcggag gagaaggcca agaccgtgtc cgactttatc gccgctcgct tcggccacga
     1981 tgtggtcgtc gcccggctgg tgacggtgat cgcgctcctg ggcacgatcc cctacgtcgc
     2041 gctccagctg cgctcgatcg gcaatgcggt ctcgctcgtg tcgggcacgc ccgtcggact
     2101 gccggccacg atcgccgccg ctgccgtgct ggcgctgttc gcgatcctgt tcg
//
LOCUS       DATNBV010000185         2533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4253877,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000185 DATNBV010000000
VERSION     DATNBV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4253877"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VN627_04505"
     CDS             <1..455
                     /locus_tag="VN627_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HXD02550.1"
                     /translation="IPKPNGGGERALGIPTIRDRVVQTAAKLVLEPIFEADFDDGAYG
                     YRPGRSATDAIKEVHRLICRGYTDVVDADLSKYFDTIPHADLLKSVARRIADRHVLWL
                     IKLWLKAPIEERDGDGKRHMSGGKTSTCGTPQGGVATPPTMVQNFRLI"
     gene            773..2470
                     /locus_tag="VN627_04510"
     CDS             773..2470
                     /locus_tag="VN627_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HXD02551.1"
                     /translation="MTSIRAAIYARVSSEQQAADSTINSQIAALEARLAQDGLSLMPD
                     EHFCDEGYSGATLVRPALERLRDAVAAGHLDRIYVHSPDRLARRYAYQVLLIDEFRRA
                     GVEVVFLNRSIGLSPEDDLLLQVQGMLAEYERAKILERSRRGKRHAAHQGEVSVLSGA
                     PYGYRYIGKHIGGGVARYEVQADEAHIVRQIFQWIGHERVSIGEVCRRLQAQGCPTRS
                     GKPAWDRSTVAGILKNPAYAGAAAFGKTRVGPLPARLRPVRGGAEQPRRAYGVYDVAP
                     EAWISVPVPPLIDAALAEAAVEQLAENRQRSRQSARGRRYLLQGLVVCRQCGYAYYGK
                     AVSLASAKGQRRHYAYYRCCGSDAYRFGGERLCANQQVRTDRLDEAVWQEVERVLEDP
                     SWIAAEYERRLVQARDPAAADLASLEGQIAKLRRSLDRLIDGYAEGLIDKAEFEPRAA
                     GLRQRLRGWDEQAVRIRNEAAQRAALSLIVGRLEDFARQVRQRMAAVDWSMQRDLIRL
                     LVKRVEVDREDVTVVLRVTPSPAGPGSPNPNAGHILHHCGWSDLTVPFECLPAPCAG"
BASE COUNT          441 a          831 c          834 g          427 t
ORIGIN      
        1 tgatcccgaa gccgaacggc ggaggcgagc gtgcgctcgg cattccgacc atacgcgatc
       61 gggtggttca gaccgccgcc aagctcgtgc tggaaccgat tttcgaggcg gatttcgatg
      121 atggagccta tggctaccga cccggtcgca gtgcgaccga cgcgatcaag gaagtgcacc
      181 ggctgatctg ccggggctat accgatgtcg ttgacgccga tctatcgaag tatttcgata
      241 cgatcccgca tgcggacctc ctcaaatcag tggcccgccg catcgccgac cggcacgtgc
      301 tgtggctgat caagctgtgg ctgaaggcgc cgatcgagga gcgggatggc gacgggaaac
      361 ggcacatgag cggcggcaaa accagcacat gcggcacgcc ccaaggtggc gtcgccactc
      421 cacccacaat ggtgcaaaac ttccgtttga tctgacgata acgatcccac gggatcggct
      481 gcgcccgcac tatcgcgacg gcttcgcagg cgcaccgttg atggcgtgtc ggccgtgcaa
      541 tccgcatcgg cccggtcacg gaggcgccga tgccccggcg cagaacaagc tttcgccctc
      601 tcatagtccg ggtgagccat gaggccaacc ggctcgaaca gcacgtcatg gccgacgcct
      661 ttgagcggct gttgccgatc attgaacgcc ggcttcgacc ccagcccgac acctgcgact
      721 cacctggcca ggctacatcc aacaccacat tcatttctag aaagacacac acatgacgtc
      781 tatacgcgcc gccatctatg cccgcgtctc ctccgagcag caggccgccg acagcaccat
      841 caacagccag atcgcagccc tggaggcccg cctcgcccag gacggcctgt cgctgatgcc
      901 cgatgaacat ttctgcgacg aaggctacag cggcgccacc ctggtgcgac ccgccctcga
      961 gcgtctgcgc gacgctgtcg ccgccggtca cctcgaccgt atttacgtgc attcgcccga
     1021 ccgccttgcc cgccgttatg cctaccaggt gctgctgatc gacgagtttc gccgcgccgg
     1081 ggtcgaggtc gtgttcctca accgctcgat cggcctctcg ccggaggacg accttctgct
     1141 gcaggtgcag ggtatgctcg ccgaatacga gcgtgccaag atcctggagc gcagccgacg
     1201 cggtaagcgg cacgccgccc accagggcga ggtgagcgtg ttgtcgggcg cgccctatgg
     1261 gtaccgctat atcggcaagc acatcggcgg cggggtggcc cgctacgagg ttcaggcgga
     1321 cgaggcccac atcgtgcgtc agatctttca gtggatcggc cacgagcgcg tcagcatcgg
     1381 cgaggtgtgc cggcggctgc aggcccaggg ctgtccgacc cgcagcggca agccggcctg
     1441 ggaccgctcg accgtggcgg gcatcctcaa gaaccccgct tatgccggcg cggcggcctt
     1501 cggcaaaacc cgggttgggc cgctgccggc ccgcctgcgt ccggtgcgcg ggggcgccga
     1561 gcagccccgc cgcgcctatg gcgtctacga cgtggccccg gaggcgtgga tcagcgtgcc
     1621 ggtcccaccc ctgatcgacg cagcgctggc cgaggcggcg gtcgagcagc tggcggagaa
     1681 ccgacagcgt agccgccaaa gcgcccgcgg ccggcgctat ctgctgcaag ggctggtggt
     1741 ctgtcggcag tgcggttatg cctactatgg caaggcggtc agcctggcct cggccaaagg
     1801 ccagcggcgc cattatgcct actatcgttg ctgcggcagt gatgcgtatc gtttcggtgg
     1861 cgagcgcctg tgcgcaaacc agcaggtgcg caccgatcgg ttggacgaag cggtgtggca
     1921 agaggttgag cgcgtgcttg aagatcccag ttggatcgcg gccgagtatg agcggcgcct
     1981 cgtccaggcc cgcgaccccg ccgccgccga tctggccagc cttgagggtc agatcgccaa
     2041 gctgcgccgc agcctggacc ggctgatcga cggctatgcg gaaggcttga tagataaggc
     2101 cgaatttgag cctcgcgccg ccgggctgcg ccagcggctg cggggctggg acgagcaggc
     2161 agttcggata cgcaacgagg cggcgcagcg cgcagcactg tcgctgatcg tgggccggtt
     2221 ggaagacttt gctcgccagg tgcggcaacg gatggcggcg gtagactggt ccatgcaacg
     2281 cgacctgatc cggctgctcg tcaagcgcgt cgaggtcgat cgagaggacg tcaccgtggt
     2341 cctgcgtgta acaccatcac ctgccggtcc aggatcacca aaccccaatg ctgggcatat
     2401 tttgcaccat tgtgggtgga gtgatctcac cgtgcctttc gaatgtcttc ctgcaccatg
     2461 tgctggatga atggttcgag accgaggtga aaccgcgtct cagaggggcg tgcacccttg
     2521 ttcggttcgc cga
//
LOCUS       DATNBV010000186         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1084337,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000186 DATNBV010000000
VERSION     DATNBV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084337"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VN627_04515"
     CDS             <1..702
                     /locus_tag="VN627_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXD02552.1"
                     /translation="EEPFIAAQFQVRSIPTVYAVFQGQPVADLTSARTESQLKGMLDQ
                     LLAKLPIQPGGAPAPDLAPLLAMGDEVLSAGDGERAAGIYAQIMELAPDNAAALAGLV
                     RALTMAGHLDEAEAALAETDAKLAEEPAVAQARAALELARSKPEDSELAALRAAAAER
                     PADMDAQLAFAGGAFAAGERDVAAETLLRMIAADRTWNEGAARAKLLQIFEAVGLEDP
                     WVAAQRRKLSTILFG"
     gene            714..1328
                     /locus_tag="VN627_04520"
     CDS             714..1328
                     /locus_tag="VN627_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXD02553.1"
                     /translation="MASQRISIFPLPGAVLYPGLQLPLHIFEPRYRAMISDALARDRR
                     IGMIQPQQPAEGAPLYAVGCVGKIGEVEALPDGRFNLILEGEARFRILREREVTTPFR
                     QIDAELFEELEHETLAPVERAAFERESRRFAAIQGYRVDWESVARLDDQSLINGVAQV
                     APFDPAAKQALLEAPDLSSRCELIIQLMQFFARRDSDDDRATLQ"
     gene            complement(1394..>2032)
                     /locus_tag="VN627_04525"
     CDS             complement(1394..>2032)
                     /locus_tag="VN627_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02554.1"
                     /translation="VIAAELALDRDAAARGQWTALAAAAAPAAVVFTPAPGTRIAWAR
                     DWLKGRANPPVAERWQSRSVWSSCDGALVVSRGTWRAGDRGGWFARVWQRQGDGSYKW
                     LVTRSGAEVREDAEPDMIAAQVAECPARKARDEDEPHRKSRPPKPARAKDLPPLDPLR
                     HGGASPDGSLRWEASVGGDGGPHLTAVWRKDGAEQALPVDGMAGSSPPPNLR"
BASE COUNT          295 a          787 c          658 g          292 t
ORIGIN      
        1 gaggagccgt tcatcgccgc ccagttccag gtgcggtcga tcccgacggt ctatgccgtg
       61 ttccagggcc agccggtcgc cgatctcacc agtgcccgca ccgagagcca gctcaagggc
      121 atgctcgacc aattgctcgc caagctgccg atccagccgg gcggcgcgcc ggcgcccgat
      181 ctcgcaccgc tgctggcgat gggcgacgaa gtgctgtccg ccggggatgg cgagcgcgct
      241 gccgggatct atgcccagat catggaactg gcgcccgaca atgccgctgc cctcgccggg
      301 ctggtccgcg cgctgaccat ggccggtcat ctcgacgaag ccgaagccgc cttggccgaa
      361 accgatgcca agctcgccga ggaacctgcc gtcgcccagg cccgcgccgc gctggaactc
      421 gcgcgcagca agccggagga cagcgaactc gccgccctgc gcgccgccgc ggccgagcgc
      481 ccggccgaca tggacgccca gctggccttc gccggcggcg cctttgcggc gggcgagcga
      541 gacgtcgccg ccgagaccct gctgcggatg atcgcggcag accgcacctg gaacgagggc
      601 gccgcccgcg ccaagctgct ccagatcttc gaggccgtgg gcctggagga tccctgggta
      661 gcggcgcagc ggcgcaagct ttccaccatt cttttcggct gacccggatc gccatggcca
      721 gccagcgcat ctcgatcttc ccgctgcccg gtgcggtgct ctatcccgga ctgcaattgc
      781 cgctgcacat cttcgaaccg cgctaccggg caatgatttc cgatgccctc gcgcgggaca
      841 ggcggatcgg catgatccag ccgcagcaac ccgccgaggg cgccccgctc tatgcggtgg
      901 gctgcgtggg gaagatcggc gaggtcgagg ccctgcccga cggtcgcttc aacctgatcc
      961 tcgaaggcga ggcccgcttc cgcatcctgc gcgagcgcga ggtgaccaca ccgttccggc
     1021 agatcgatgc cgagctgttc gaggaactgg agcacgagac cctggcgcca gtggagcgcg
     1081 ccgccttcga acgcgaatcc cgccgcttcg ccgcgatcca gggctaccgg gtggattggg
     1141 aatcggtcgc ccggctcgac gaccagagcc tgatcaacgg cgtcgcccag gtcgccccgt
     1201 tcgatccggc cgccaagcag gccctgctcg aggcgcccga cctctcatcc cgctgcgagc
     1261 tgatcatcca gctgatgcag ttcttcgccc gccgcgattc ggacgacgac cgggcgacgc
     1321 tgcagtagcg tttggccgcg ccgtcgcacc cccccatggg gatgcacacc gcagctttgc
     1381 agcgacaagc tgcctagcgc agattgggtg ggggtgaaga ccccgccatc ccgtcaaccg
     1441 gcagcgcctg ctccgcccca tccttgcgcc acaccgcagt caagtgcggc ccgccatcgc
     1501 cgcccacact ggcttcccag cgcagcgaac catcgggcga tgccccgcca tggcgcagcg
     1561 gatcgagcgg cggcaggtcc ttcgcgcggg ccggcttcgg tggcctgctc ttccggtgcg
     1621 gctcatcctc gtccctcgcc ttgcgcgccg ggcactcggc cacctgcgcc gcgatcatgt
     1681 cgggctcggc gtcctcgcgg acctcggcgc cggacctggt gaccagccat ttgtaactgc
     1741 catcgccctg ccgctgccac acccgcgcaa accagccgcc ccggtcgccg gcccgccagg
     1801 tgccccggct caccaccagc gccccgtcgc agctcgacca gaccgagcgc gattgccacc
     1861 gctctgcaac cggcggattg gcccggccct tcagccagtc acgcgcccag gcgatgcggg
     1921 ttccgggcgc gggcgtaaac accacagccg ccggcgccgc tgccgctgcc agtgccgtcc
     1981 attgcccgcg cgccgccgca tcgcggtcca gcgccagttc ggcggcgatc ac
//
LOCUS       DATNBV010000187         5197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3940893,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000187 DATNBV010000000
VERSION     DATNBV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3940893"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VN627_04530"
     CDS             <1..189
                     /locus_tag="VN627_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02555.1"
                     /translation="YRKLRCAAWCLPSVALFFPVPMVPWATRARDSLPLLYRAFPLWQ
                     GLHAATRAASYVCFAGEQ"
     gene            328..1497
                     /locus_tag="VN627_04535"
     CDS             328..1497
                     /locus_tag="VN627_04535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXD02556.1"
                     /translation="MSTPVVINAVRTAVATARKGTLANTSAEQLGETILRAALERSGV
                     DPLLVDDVIFAESMYGGGAVARYAAVKVGLMQAGGMAVNRHCAGSLSSVGVAAGSIIA
                     GMERLVIAGGVQASSMTPMTQFRKALTNEFETRMAPTHPDTPGAPGSDMSITVGWNTA
                     QAVGLTREEMDAWAARSHARAIAAIDAGQFKDEITPVEALQVDGSTKVFDVDEHPRRD
                     STLEKMATLKVIHPEIEGFSITAGNSSGVNDAASALTIASADFAKGEGHDPIAKILGW
                     TAKGVNPFDTGLGAIESIKKLLERTGRKASDIALWEINEAFASVPIAACKLLGLDDNL
                     VNISGSGCSIGHPIAASGGRMLTTLINDLKRRGGGLGVAAMCAGGGQGGAVLIEV"
     gene            complement(1571..3397)
                     /locus_tag="VN627_04540"
     CDS             complement(1571..3397)
                     /locus_tag="VN627_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02557.1"
                     /translation="MRAGPEIADAVIPTGAGNGCPQETAAEATAALRLATLVTLLLIF
                     ASAVGAQLIFPSLRYPGNLLYLTQFSARRQDIPVGFGLAGLFVLTGLLRRKPPSTAAR
                     LPGLTMRRALAVVAATVLVTWAIRTMVLFDHDFTRDEQMASFDAAIYGAGHLFERIPQ
                     GMRALYIPLNSLFLFPVSDHSAWVSSYLPVNALIRGLAGKFLPDAMVSALLTGAAGLL
                     LWRIARRVWPDDRTTQMVVLACFAGSSQVVLTGGATFAMSTHLALNLLWLACFLRGTR
                     GGHAGAMLTGLLATGIHQPVFHPLFVLPFLDLLRRRGEWRTLACYVAAYGLIGLFWLA
                     WPHYVLAEIAPGPGPDAVIAATQTGLIDRLLLLLTPPNLDALWVMAGNVLRFVTWQHA
                     LLVPLALVGVLRCWRGDDLARALALGVALLLVAMTILLPPQGHGWGYRYLHGLIGNCC
                     LLAGYGWRWLAQRGAAPVRAMQLATVVSVAVLLPLHAIMVRGFIAPFAEARAAVARTP
                     ADVVVFETGKIPFDSNFVVNRADLSNRPLVLLGDMLAPSDLAALCPGRTIAFANASQF
                     NDLAAYYEEPPTAGPGKHQWALMRVAERAGCRVVPFRRDQGA"
     gene            3578..3805
                     /locus_tag="VN627_04545"
     CDS             3578..3805
                     /locus_tag="VN627_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HXD02558.1"
                     /translation="MADNTSKRGAGDRNRVAAGQGYELRYFARKHGLTTAQANDLIRR
                     IGNDRAKLNEAAVRLRRSSSGKDDGTSQASA"
     gene            3840..4247
                     /locus_tag="VN627_04550"
     CDS             3840..4247
                     /locus_tag="VN627_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HXD02559.1"
                     /translation="MADSRTAPAPALPHTFGAAMLRGLRCSCPRCGSARLFARWLKPV
                     DSCPACGIDWTPQRADDFPAYIAIIVTGHLLAPIMIALALDYALQPLVLAAVLFPSAI
                     AMMLGLLQPAKGLVIALQWWHGMHGFVRERVQA"
     gene            complement(4259..4720)
                     /locus_tag="VN627_04555"
     CDS             complement(4259..4720)
                     /locus_tag="VN627_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXD02560.1"
                     /translation="MSNDAPPTLPRPMQTNIAFLATDIGRLFRKRFDTSARAVGVTGP
                     QWRVLAALSFHPGITQAVLAGLLDVEAITVGRMIDRLQKSGLVERRPAPADRRAWLLF
                     ITPAAEKLLGDLQKVAEGLMAETLANFAPEEFDEMVKLLNRLRDNLVAQVA"
     gene            complement(4798..>5197)
                     /locus_tag="VN627_04560"
     CDS             complement(4798..>5197)
                     /locus_tag="VN627_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXD02561.1"
                     /translation="GALQARIADPRFKLAKTYLVQVEGEPNEAALDRLQRGVDLTDGR
                     TRPAEARRIDPPALWPRDPPVRFRKSVPDCWIELTIREGRNRQVRRMTAAVGHPTLRL
                     VRWRVGEWTLAGLAPGEWREIAVSPPERRA"
BASE COUNT          927 a         1758 c         1732 g          780 t
ORIGIN      
        1 tatcgcaaac tgcgctgtgc agcttggtgc ctgccttcag ttgcattgtt ctttcccgtg
       61 ccaatggtgc cgtgggccac gcgggcccgc gattcgttgc cgttgcttta tcgcgctttc
      121 cccctctggc aaggtctcca cgcggccacg cgcgcggcat cctatgtatg cttcgccggc
      181 gagcagtgaa cctcgggtgc cgcccacatc atcggacctg tgttgttagc atgcgcgccg
      241 ccggaggccc ttgcgctgcc ttccgccagc cccataaccg caagataggc aaaaattcgc
      301 ccgacggggc gcaagaagcg aggaatcatg tccacacctg ttgtcatcaa cgccgtccgg
      361 acggctgttg ccacggcccg caagggaacg ctggccaaca cctcggccga acagctgggc
      421 gagaccatcc tgcgcgccgc gctggagcgg tccggggtcg atccgctgct ggtggacgac
      481 gtgatcttcg ccgaatcgat gtacggcggc ggcgccgttg cccgctatgc cgcggtcaag
      541 gtcggcctga tgcaggccgg cggcatggcg gtgaaccgcc attgcgcggg cagcctgtcc
      601 tcggtcggcg tagcagctgg ttcgatcatc gccggaatgg agcggctggt catcgccggc
      661 ggcgtccagg ccagctcgat gaccccgatg acccagttcc gcaaggcgct gaccaatgaa
      721 ttcgaaaccc gcatggcgcc gacccatccc gatacgccgg gcgcgcctgg cagcgacatg
      781 tcgattaccg taggctggaa cacggcccag gccgtgggcc tgacgcgcga ggagatggac
      841 gcctgggctg cccgctccca cgcccgcgcc attgcggcga tcgatgccgg gcagttcaag
      901 gacgagatta ccccggtcga ggcgctgcag gtcgacggtt cgaccaaggt gttcgacgtc
      961 gacgaacatc cccggcgcga cagcacgctg gaaaagatgg cgacgctcaa ggtcattcac
     1021 ccggaaatcg aaggcttctc gatcactgca ggcaattcca gcggcgtcaa tgacgcggct
     1081 tcggccctga cgattgccag cgcggatttc gcgaagggcg aaggccacga tccgatcgcg
     1141 aagatccttg gatggactgc caaaggcgtc aatccgttcg acactggcct tggcgcgatt
     1201 gaatcgatca agaagctgct ggagcggacg ggccgcaagg cgagcgacat cgccctctgg
     1261 gaaatcaacg aggccttcgc ttccgttccg atcgcggctt gcaagctgct cgggcttgac
     1321 gataacctcg tcaacatctc gggcagcggc tgttcgatcg gccacccgat cgctgcctcg
     1381 ggcgggcgta tgctgacgac gctgatcaac gacctcaagc ggcggggtgg cggccttggc
     1441 gtcgcggcga tgtgcgccgg cggcggccag ggcggtgcgg tgctgatcga agtctgatca
     1501 acacggcgcc gaatggcacg aacaacggcg ggggcaacca ggcgcttgct cccgccgttt
     1561 tcgattcagg ctaagcgcct tgatcccgcc ggaacggtac cacgcggcag cccgcacgct
     1621 cggccacccg catcagggcc cactggtgct tgcccggtcc cgcggtcggc ggttcctcat
     1681 agtaggcggc aagatcattg aactggctgg cgttggcgaa tgcgatggtc cggcccgggc
     1741 agagcgcggc caggtccgag ggagccagca tgtcgcccag caggacgagc ggacggttcg
     1801 acaggtcggc ccggttgacc acgaaattgg aatcgaacgg gatcttgccg gtttcgaaga
     1861 cgaccacgtc ggccggcgtc cgcgcgaccg ccgcacgggc ttcggcaaag ggagcaatga
     1921 acccgcggac catgattgcg tggagcggca gcagcactgc gaccgaaact acagtcgcca
     1981 gttgcatcgc ccgcaccggc gccgccccgc gctgcgcgag ccagcgccag ccataaccgg
     2041 ccagcaggca gcagttgccg atcaggccgt ggaggtagcg gtagccccag ccgtggcctt
     2101 ggggcggcag caggatcgtc atggccacaa gcagcagcgc cacgccaagg gccagcgcgc
     2161 gggcgagatc atcgccccgc cagcaccgga gcacgcccac cagtgccagc ggcaccagca
     2221 gtgcatgctg ccaggtgacg aaacgcagca cgttgccagc catgacccac agcgcatcga
     2281 gattgggcgg ggtcagcagg agcagaagcc gatcgatcag cccggtctgg gtggcggcga
     2341 tcaccgcatc gggtccgggc ccgggcgcga tctccgccag gacgtagtgc ggccaggcca
     2401 gccagaacag accaatcagg ccataggcgg ccacgtagca cgccaacgtg cgccactcgc
     2461 cccggcggcg cagcaggtcg aggaaaggga ggacgaacag gggatggaat accggctggt
     2521 ggatgccggt ggccagcagg cccgtgagca tcgctccggc atggccgccc cgggttccgc
     2581 gcaggaagca ggcgagccac agcaggttga gcgccaggtg cgtgctcatg gcgaaggttg
     2641 ccccgccggt cagcaccacc tgcgacgagc cggcgaagca ggccagcacc accatctgcg
     2701 tcgtgcggtc atcgggccag acccgccgcg caatgcgcca cagcagcagg ccggcggcgc
     2761 cggtgagcag cgccgaaacc atggcatcag gaaggaactt gccggcaagg cccctgatca
     2821 gggcattgac cggcagatag ctcgacaccc aggccgaatg atcgctcacc gggaacagga
     2881 acagtgaatt caaggggatg tagagcgccc gcattccctg cgggatgcgc tcaaacaggt
     2941 gccccgcgcc gtaaatcgcc gcatcgaacg aggccatctg ctcgtcgcgg gtgaaatcgt
     3001 ggtcgaacag gaccatggtg cggatagccc aggtaaccag caccgttgcc gcaaccacgg
     3061 caagggcgcg gcgcatggtc agccccggca gccgggcagc agtcgacgga ggtttccggc
     3121 gcagtaggcc ggtcaaaacg aacaggccgg caaggccaaa ccccaccgga atgtcctgcc
     3181 gtctcgcgga aaactgggtc aggtacagca ggttgcccgg atagcgcagg ctgggaaaga
     3241 tcagctgcgc acccaccgcc gaagcgaaga tcagcagcag ggtgaccaag gtagccaggc
     3301 gcagggctgc ggtggcctcg gccgcggtct cttgggggca accattgcca gcgccggtcg
     3361 ggatgacggc atcggcgatt tcaggccctg cccgcattgg ctccccccct ggacggcaca
     3421 cgacggcccc tgccgcccat agggggcggc agcctggtca gcaagaccgc cccggcgatc
     3481 aagcctcccg ggaactaggc atgacacccc aacgttggca tcatgagcag gctggcatgg
     3541 cttgaagccg cccctgcaaa caggtgcgga gatgcagatg gcggacaata ccagcaagcg
     3601 cggcgcgggg gaccgcaacc gcgttgccgc cggccagggc tacgaactgc gctattttgc
     3661 gcgcaagcac ggattgacca ctgcccaggc caatgacctt atccggcgca tcggcaacga
     3721 tcgcgccaag ctcaacgagg cggccgtgcg gctgcggcgc agctcgagcg gaaaagacga
     3781 cgggacgagc caggccagcg cttgacctga cgcccgcaaa ggccgagtgg ctggccatca
     3841 tggccgactc gcgaaccgct cctgcgcccg ccctgcccca cactttcggc gccgccatgc
     3901 tgcgcggcct gcgttgctcc tgcccccgtt gcggctcagc tcggctgttt gcccgctggc
     3961 tcaagccggt ggacagctgc cccgcttgcg gcatcgactg gacgccgcag cgggcggacg
     4021 atttccccgc ctacatcgcc atcatcgtca ccggccacct gctggcgccg atcatgatcg
     4081 cccttgcgct cgattatgcg ctgcagccgc tggtcctggc cgcggtgctg tttccctcgg
     4141 cgatcgccat gatgctgggc ctgctccagc cggccaaggg cctcgtcatc gcgctgcagt
     4201 ggtggcacgg gatgcacggc ttcgttcgcg agcgcgtgca agcctgaggt aattcggctc
     4261 aggccacctg cgccaccaga ttgtcgcgca ggcggttgag cagcttgacc atttcgtcaa
     4321 attcctcggg cgcgaaattg gccagggttt cggccatcag cccttcggcc accttttgca
     4381 aatcgcccag cagcttctcg gccgccggag tgatgaacag tagccatgca cgccggtctg
     4441 ccggggccgg acgccgctcc accagccccg atttctgcag ccggtcgatc atcctaccca
     4501 ccgtgatcgc ctcgacatcg agcaggccgg caagcacggc ctgggtaatg ccgggatgga
     4561 agctgagcgc agccagcacg cgccattgcg gcccggtgac cccaaccgcc cgcgcgctgg
     4621 tatcgaaccg cttgcggaac aggcgcccga tatccgtcgc cagaaacgcg atgttggtct
     4681 gcatcggccg gggcagcgtc ggtggcgcgt cgttggacat gactgaatac ctagcagcac
     4741 accgacgcat gtaaatgcgc tggcctggct gtcccccgca gtgttcaggc ccggtgttca
     4801 ggctcggcgc tcaggtgggc tgactgcaat ttcgcgccat tcgcccggag cgagccccgc
     4861 cagggtccac tcgccgaccc gccagcgcac caaccggagc gtgggatgac cgactgccgc
     4921 cgtcattcgc cgcacctgcc ggttgcggcc ctcgcggatg gtgagctcga tccagcagtc
     4981 cggcaccgat ttgcggaacc gcaccggcgg atcgcggggc cacagtgcgg gcggatcgat
     5041 ccgccgcgcc tcggccgggc gcgtgcgccc atcggtcagg tccacgccgc gttgcaggcg
     5101 atcgagcgct gcctcgttag gctcgccttc cacctggacg agataggtct tggccagctt
     5161 gaagcgtggg tcggcgatgc gggcctggag cgccccg
//
LOCUS       DATNBV010000188         2281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2025376,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000188 DATNBV010000000
VERSION     DATNBV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025376"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VN627_04565"
     CDS             <1..160
                     /locus_tag="VN627_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXD02562.1"
                     /translation="GISGAIQHLAGMKDSKTIIAINKDEDAPIFQVADIGLVGDLFNV
                     VPELVGKL"
     gene            complement(215..1423)
                     /locus_tag="VN627_04570"
     CDS             complement(215..1423)
                     /locus_tag="VN627_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HXD02563.1"
                     /translation="MRRTATGLLAGMAVLFVMAHALRTSHPLWPWVLAFAEAAMVGGL
                     ADWFAVTALFRRPLGLPIPHTAIIPENKDRIADTMAVFLQDNFLTPEVVARRMAGMNL
                     AAAAGAWLADPHAGEGERLRAGAGELFAQVLESLDPDRLGNQVRGGLVRQLDKLDVAP
                     LLGSALAAAIADKRHMAVIESMVRWAGFTLEANEDLVRELIHRRANAILRWTGLDEKL
                     ANSVIEGLYKLLAEVIVDPAHPLRAKIEEGLAQLAWGLSHDPRMQARVQRIKRELLGN
                     PALAQWWQAVWERLRIALVARLRDPQGGLGVSLRESLAELGQTLQGDPALQDQLNRLA
                     RRTAVGLATRYGSQIVRLVSETVKRWDATTVTNRLEGAVGRDLQFIRINGTLVGGLFG
                     LGIHAVEVWL"
     gene            complement(1468..>2281)
                     /locus_tag="VN627_04575"
     CDS             complement(1468..>2281)
                     /locus_tag="VN627_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXD02564.1"
                     /translation="GGTVPLSRVAEISFGSGPTSIQRYNQQRRVFIGADLAPGVVKSQ
                     AMDAINALPVMKNLPQGVSNTAYGVDLWQQEMIRNVGAAVLAGVVLVFSVLVLLYHRI
                     VSPLVNMGSLFLAPLGGLLALGFLGKPVSMPVLIGILMLFGIVAKNSILLIDFAIEEM
                     AQGRKKFDAIVEAGHKRAVPIVMTTVAMTAGMIPTAVSLSGDAAWRAPMGIVVIGGLV
                     MSTMLTLLIVPAAFSLADGLEKRLGPALRRRFLTYEPHHGRNLPEPGAYPAE"
BASE COUNT          384 a          754 c          803 g          340 t
ORIGIN      
        1 cggcatctcc ggcgcgatcc agcaccttgc cggcatgaag gattcgaaga ccatcatcgc
       61 catcaacaag gacgaggacg ccccgatctt ccaggtggcc gacatcggcc tggtcggcga
      121 cctgttcaac gtggtgccgg agctggtcgg caagctctga ggctttgccg cagcacgaac
      181 ctcgaaaggc cccgcttgcg cggggccttt ttcgttacag ccacacctca accgcatgaa
      241 tccccagccc gaacagaccg ccgaccagcg tgccgttgat gcggatgaac tggaggtcgc
      301 ggccgacggc gccttcgagg cggttggtga ccgtggtggc gtcccagcgc ttgaccgttt
      361 cggagactag ccggacgatc tggctgccgt agcgggtggc caggccgacg gcggtgcggc
      421 gggcgaggcg gttgagctgg tcctgcaggg cgggatcgcc ctggagggtc tggccgagtt
      481 cggcgaggct ttcgcgcagg gacacgccca gcccgccctg cggatcacgc agccgcgcga
      541 cgagggcaat gcgcaggcgc tcccagacgg cctgccacca ctgcgccagg gccggattgc
      601 ccagcagttc gcgcttgatg cgctggaccc tggcctgcat ccgcggatca tgggacaggc
      661 cccaggccag ctgggccagc ccttcctcga tcttcgcgcg cagcggatgg gccggatcga
      721 cgatcacttc agccagcagc ttgtagaggc cctcgatcac cgaattggcc agcttctcat
      781 cgaggccggt ccagcgcagg atggcattgg cgcggcggtg gatcagttcg cggacgaggt
      841 cctcgttcgc ctcgagcgtg aagccggccc agcgcaccat gctttcgatc accgccatgt
      901 ggcgcttgtc ggcaatggcg gcggccagcg cgctgccgag cagcggggcg acatcaagct
      961 tgtcgagctg gcggacgagg ccgccacgga cctgattgcc gagccgatcg ggatcgagcg
     1021 attccagcac ctgggcaaag agttcccctg ccccggcgcg cagccgctcg ccttcgccgg
     1081 cgtggggatc ggccagccag gcgccggcag cggcggcaag gttcatcccc gccatgcggc
     1141 gggcgacgac ctcgggcgta aggaaattgt cctgcaggaa caccgccatc gtgtcggcga
     1201 tgcggtcctt gttttccggg atgatcgcgg tgtgcgggat cggcaggccc agcgggcggc
     1261 ggaacagggc ggtgacggcg aaccagtctg ccagcccgcc gaccatggcg gcttcggcaa
     1321 aggcgagcac ccacggccag agcggatgac tggtgcgcag cgcatgggcc atgacgaaca
     1381 gcacggccat gccggcgagc aggccggtgg cggtgcgccg catgcgccgg gcgcgatcgg
     1441 gttgggcggg agggcgcggc ggagatctca ctccgccggg tacgccccgg gttcgggcag
     1501 gttccgccca tggtgcggtt cgtaggtcag gaaccgccgt cggagggccg ggccgagccg
     1561 cttttcgaga ccatcggcca ggctgaaggc cgccgggacg atcagcaggg tgagcattgt
     1621 ggacatcacc aggccgccaa tcaccacgat ccccatcggc gcgcgccagg cggcatcgcc
     1681 cgacagcgag accgcggtcg ggatcatgcc cgcggtcatc gccacggtgg tcatcacgat
     1741 cggcacggcc cgcttgtggc cagcctcgac gatggcatcg aacttcttgc ggccctgggc
     1801 catctcctcg atcgcgaagt cgatcagcag gatcgagttc ttggccacga tcccgaacaa
     1861 catgagtatg ccgatcagca ccggcatcga caccggcttg ccgaggaagc cgagggcaag
     1921 cagcccgccg agcggcgcga ggaacagcga gcccatgttg accagcggcg acacgatgcg
     1981 gtgatagagc aggaccagca ccgagaagac cagcaccacc cctgccagta cggccgcgcc
     2041 gacattgcgg atcatttcct gctgccacag gtcgaccccg taggctgtat tggagacgcc
     2101 ctgaggcagg ttcttcatga cgggcagggc gttgatggca tccatcgcct ggctcttgac
     2161 gacgccggga gcgaggtcgg ccccgatgaa cacgcgccgc tgctggttgt agcgctggat
     2221 gctggtgggc ccggagccga agctgatctc ggccacccgg ctgaggggga cagtgccgcc
     2281 a
//
LOCUS       DATNBV010000189         6541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3609883,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000189 DATNBV010000000
VERSION     DATNBV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3609883"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VN627_04580"
     CDS             <1..206
                     /locus_tag="VN627_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HXD02565.1"
                     /translation="YLDSTGPVAGEAVQGFMSDNLEALVERALAHGGTTPDPIREVPG
                     FGLRVVFVVDPEGHVNEVIQMNA"
     gene            216..1274
                     /locus_tag="VN627_04585"
     CDS             216..1274
                     /locus_tag="VN627_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02566.1"
                     /translation="MVRELAENFRPVAPPQLIDDAYSPDQHARLLQVVRDNGPWPLIL
                     AENFKTPEEVIATISGSIPEGVKLTWDMIGLNPVFRGYLARGGTCFYPEIEDCYYNSR
                     FLELVRNYWGCQYAEPETFLFNIQGPTPIGGPPHLDGTVFRGLTMENTPLWLLLTMAK
                     SCLFDRWRSKKGQVIAWYYNGKIGGGFNCWPNGPSAEPMQINAPMWGKAVVVENEMMF
                     HHGQATGPVALRNPQGLDISSTFGADPEDPTGWQIETFGKVNQKVPAGEMRFLVHWGA
                     RLFTDMADLKRTYEHTDDITAETAIGMLIDDMKQRGVEFEVPTDPLHDNAFVGVLAQA
                     YNIGGPTNIPPDALDEAA"
     gene            1346..2335
                     /locus_tag="VN627_04590"
     CDS             1346..2335
                     /locus_tag="VN627_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HXD02567.1"
                     /translation="MLRNDIDREAYLRMCKIRGFEEAVTALITEGEVPGACHSSVGQE
                     AAIVGACMALRNDDYVSGTHRSHGHPIGKGAALKPLMAEILGKATGVCKGRGGSMHLT
                     DKSVGSIGESAIVGGGIPIATGAALTAQVKGTDQVALTFFGDGASNEGVLAECLNMAA
                     VWKLPVIFFCENNGYAAVTPARDSHGQPDVARRADGYGVPGVVVDGQDMDAVLAATRT
                     AVARARAGEGPTLIEAKTYRFDEHCVGLFIKGGYRTQEEVQFFMNERDPLKLFRQVML
                     DRGHGEDDINAVEAEARGLVAEAVQFAKDSPYPEPEAAFELVHATPLATPIRR"
     gene            2347..3321
                     /locus_tag="VN627_04595"
     CDS             2347..3321
                     /locus_tag="VN627_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HXD02568.1"
                     /translation="MTEQTTQRMTYLDALGLAQREEMARDENVIIIGEDITLYAAGGA
                     YGNIDSKRVRSAPISECGFAGMAVGAAITGLRPIVDLTIANFVYLASDPIINQAARLR
                     YMTGGQFNVPVVYRASMWHSQANAAQHSDRPYPMFMNVPGLKVVCPATPEDAKGLLKA
                     AIRDDDPVLVFEDNDLWGQKGEVPLDTDFVVPIGKARTHREGSDVTIVTVSGSLRFAL
                     KAADDLAKDGISVDLVDLRTVLPIDSEAILNSLGKTGRIVIVDPAHRTCGAAAEIAAI
                     VAEDGFDLLRKPVRRVVTPDVPIPFAPTMEKPLYPSREAIVAAVRSLM"
     gene            3353..3592
                     /locus_tag="VN627_04600"
     CDS             3353..3592
                     /locus_tag="VN627_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HXD02569.1"
                     /translation="MRVKVVMPKWGTGMNEGTVVKWLKAVGDRVEAGDMLVEIETSKS
                     TQEMEAPASGTLAEILLPEGEEAEVRTHIATIETD"
     gene            3595..4506
                     /locus_tag="VN627_04605"
     CDS             3595..4506
                     /locus_tag="VN627_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD02570.1"
                     /translation="MATREILGPDISGKIALVTGSSRGIGRAVAQRLAVAGAAVVVTG
                     RSSGQPAVGKRWGQDHVVPGTLEETVALIEQAGGKAVSLAADLEDLDQVRNLVDRAAE
                     LAGGGIDILVNNAGFVDFAPVADMAFDTFERTLTHYVRAPFLLSQAAVRHMREAGAGW
                     IVNVSSQDALPPSRPYPDHEKVRGYHVYAAAKAALNRLTQGMAAELVDENIAVNVIGP
                     STAIRTPGADALIPAEFPTEDVEYLAEAVLAMCHLPAAERTGLIGYSMHIPWHYGIPV
                     RSLDGKTELPRRAPPAWSHPAIAASGE"
     gene            4532..5380
                     /locus_tag="VN627_04610"
     CDS             4532..5380
                     /locus_tag="VN627_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD02571.1"
                     /translation="MTPEQAFTGGTAVITGAGSGIGEGLARVAARHGMNVVLADIAQD
                     RVERVAAEIAATGARALAVVTDVTDIAALERLADAAWDRFGGVRLAINNAGIETIGYS
                     WELPDATWDKAIDINIRGVVHGSRIFARRMLEAGQAGYIANVASIGSLGMMPTQAPYM
                     MSKHAVLSFTECLFLEMQVRNAPIQVSAVLPAQVATRIFEDAPADLGSPMVSRQREMM
                     HAMITQMGITPEQAGEVILAGIASGEFWVSTHPETTAAMAAARGAYLAALSTPSLPEE
                     VRALVE"
     gene            5457..5588
                     /locus_tag="VN627_04615"
     CDS             5457..5588
                     /locus_tag="VN627_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02572.1"
                     /translation="MTDEYLVGMLFKRLLLLRNLHGGPDPALARLARGRAHRASEST"
     gene            5590..>6541
                     /locus_tag="VN627_04620"
     CDS             5590..>6541
                     /locus_tag="VN627_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HXD02573.1"
                     /translation="MAANDPMVVGIGRSHYSRNSGRTTWGMAVESCRLAVEDAGMAMS
                     DVDGIVEFNTADSTGSLNVAYGLGLPGIRYPVDLQNGGNIVTFAVALAAMAVQTGKCK
                     AILVYRSLNSRSGKRLGTFTGDLAIGGDDQYGAPHGYLAPGQWFAMYCRRYMHEFGTT
                     NEDLGEVAIVTRQHAVKNDWAMMRDPITMADYMASRWIYDPFRLYDCALEADGACAIL
                     ITSRERAQDLRQAPVQVVDEETFMGRHPDSWSDFGHLFSAHSAPPLWQRTGLTARDMD
                     FACIYDCFTFTTIAVMEDYGFCEKGGAGEYFRSGKATYGGD"
BASE COUNT         1162 a         2152 c         2190 g         1037 t
ORIGIN      
        1 agtacctgga ttccaccgga ccggttgccg gcgaagccgt gcagggcttc atgtccgaca
       61 acctcgaggc gctggtcgag cgggcactgg cgcacggcgg cactacgccg gatccgatcc
      121 gggaagtgcc cggcttcggc ctcagggtcg tcttcgtcgt cgatcccgag ggccatgtga
      181 acgaagtcat ccagatgaat gcgtgaggtg ctgtcatggt acgtgaactt gccgaaaact
      241 ttcgccccgt cgcgccgccg cagctgatcg acgatgccta ttcgccggac cagcacgcaa
      301 ggctgctgca ggtggtccgc gacaatggcc cctggccgct gatcctggcc gagaacttca
      361 agacgcccga ggaagtgatc gccacgattt cggggtcgat ccccgaaggc gtgaagctga
      421 cgtgggacat gatcggactg aacccggtgt tccgcggcta tcttgcccgc gggggcacct
      481 gcttctaccc cgagatcgag gactgctact acaacagccg cttccttgag ctcgtgcgca
      541 attactgggg ctgccagtac gccgaaccgg aaaccttcct gttcaacatc caggggccga
      601 ccccgatcgg cgggccgccg cacctcgacg gcaccgtgtt ccgcggcctg accatggaga
      661 acaccccgct ctggctgctg ctgacgatgg ccaagtcgtg cctgttcgac cgttggcggt
      721 cgaagaaggg ccaggtcatc gcctggtact acaacggcaa gatcggcggc ggcttcaatt
      781 gctggccgaa cgggccgagc gccgaaccga tgcagatcaa cgcgccgatg tggggcaagg
      841 cggtcgtcgt cgagaacgag atgatgttcc accatggcca ggccaccggc ccggtcgccc
      901 tgcgcaaccc gcaggggctg gacatcagct ccaccttcgg cgccgatccc gaagacccga
      961 ccgggtggca gatcgaaacc ttcggcaagg tcaaccagaa ggttccggcc ggcgagatgc
     1021 ggttcctcgt ccactggggc gcgcgcctgt tcaccgacat ggcggatctc aagcgcacct
     1081 acgagcacac cgacgacatc accgctgaaa cggcgatcgg gatgctgatc gacgacatga
     1141 agcagcgcgg cgtcgagttc gaagtgccga ccgacccgct ccacgacaac gcgttcgtcg
     1201 gcgtgctggc ccaggcctac aacatcggcg ggccgaccaa cattccgccg gacgcgctcg
     1261 acgaggcggc ctgaggacca agacatccgg cggggtcgat tgaagcggcc ccgcccaaaa
     1321 aacttccgcc gggaagggga gagccatgct ccgcaacgac atcgaccgcg aggcctactt
     1381 gcggatgtgc aagatccgcg gcttcgaaga ggccgtcacc gccctcatca ccgaaggcga
     1441 ggtgccgggc gcctgtcatt ccagcgtggg ccaggaagcc gccatcgtcg gcgcctgcat
     1501 ggcgctgcgc aacgacgact atgtttcggg cacgcaccgc agccacggcc atccgatcgg
     1561 caagggggcc gcgctcaagc cgctgatggc cgaaatcctc ggcaaggcca cgggcgtgtg
     1621 caaggggcgc ggtggttcga tgcacctgac cgacaagtcg gtcggctcga tcggggaatc
     1681 cgcgatcgtg ggcggcggta tcccgatcgc caccggggcg gcgctcaccg cgcaggtcaa
     1741 ggggaccgac caggtcgcgc ttaccttctt cggcgatggc gcctcgaacg agggcgttct
     1801 ggccgagtgc ctcaacatgg cggccgtgtg gaagctgccg gtgatcttct tctgcgagaa
     1861 caacggctat gccgctgtga cgcccgcgcg cgacagccat ggccagccgg acgtggcgcg
     1921 ccgggccgat ggctacggcg ttcccggcgt ggtcgtcgac gggcaggaca tggatgccgt
     1981 gctcgcagca acgcgtacgg ccgttgcccg cgcccgcgcc ggtgaaggcc ccacgctgat
     2041 cgaggccaag acctatcgct tcgacgaaca ctgcgtcggt ctgttcatca agggcggcta
     2101 ccgcacccag gaggaagtgc agttcttcat gaacgagcgc gatccactca agctgtttcg
     2161 ccaggtcatg ctcgatcgcg ggcacggcga ggacgacatc aacgcggtgg aggccgaggc
     2221 ccgcgggctg gtggcagagg cggtgcagtt cgccaaggac agcccctatc ccgagcctga
     2281 ggcagccttc gagctggtcc acgccacgcc gcttgcgacc ccgatccggc gctgagagaa
     2341 cccgacatga ccgaacagac cacccagcgc atgacctacc ttgacgccct gggccttgcc
     2401 cagcgcgagg aaatggcgcg tgacgagaac gtcatcatca tcggcgagga catcacgctc
     2461 tatgccgccg gcggcgctta cggcaatatc gacagcaagc gcgtgcgcag tgcgccgatc
     2521 tccgagtgcg gctttgccgg catggccgtg ggcgcggcga tcacgggcct caggccaatc
     2581 gtcgacctga ccatcgccaa cttcgtctat ctcgcatcag atccgatcat caaccaggcc
     2641 gcgcgcctgc gctacatgac cggcggccag ttcaacgtgc cggtcgtcta ccgcgcgtcg
     2701 atgtggcaca gccaggccaa tgccgcgcag cattcggacc ggccctatcc gatgttcatg
     2761 aacgttccgg ggctgaaggt cgtctgcccg gcaacgcccg aggatgccaa gggcctgctc
     2821 aaggccgcga tccgcgatga cgatccggtg ctggtgttcg aggacaacga cctgtggggc
     2881 cagaagggcg aagtgccgct cgatactgat ttcgtcgtgc cgatcggcaa ggcccgcacc
     2941 caccgcgagg gcagcgacgt gaccatcgtg acggtttccg gctcgctccg gttcgcgctg
     3001 aaagcggctg acgatctcgc gaaggacggc atcagcgtcg acctggtcga tctgcgtacc
     3061 gtgttgccga tcgacagcga ggcgatcctc aattcgctgg gcaagacggg gcggatcgtc
     3121 atcgtcgacc cggcacaccg cacctgcggc gcggccgcgg aaatcgccgc cattgtcgcc
     3181 gaggacgggt tcgacctgct gaggaagccg gttcggcggg tggtgacgcc ggacgtgccg
     3241 atcccgtttg cgccgacgat ggaaaagccg ctctacccca gccgcgaagc cattgtcgcg
     3301 gccgtgcgct cgctgatgtg accggcagcg atcccggttc aggagaattc cgatgcgtgt
     3361 aaaggtcgtc atgcccaagt ggggcactgg aatgaacgaa ggcaccgtgg tcaagtggct
     3421 gaaggccgtt ggtgaccgcg tcgaggccgg cgacatgctg gtcgagatcg aaacctcgaa
     3481 atcgacccag gaaatggaag cgcccgcatc ggggacgctg gccgaaatcc tcctgcccga
     3541 aggcgaggaa gccgaagtgc gcacgcacat cgccacgatc gaaacagact gaccgtggcg
     3601 acgcgcgaga tcctcggtcc ggacatttcg ggcaagattg cgctggtgac cggcagcagc
     3661 cgcggcatcg gccgcgcggt ggcccagcgc cttgcggtgg caggcgcagc cgtggtggtg
     3721 accgggcgct cgtcgggcca gccggccgtg ggaaagcggt gggggcaaga ccatgtcgtg
     3781 cccggcacgc tcgaggaaac ggtcgcactg atcgaacagg ccggcgggaa ggcggtatct
     3841 ctggccgccg acctggagga tctcgaccag gtccgcaacc tggtcgaccg cgcggccgag
     3901 ctggctggcg gcgggatcga tatcctcgtc aacaatgccg gtttcgtcga cttcgctccg
     3961 gtggcggaca tggcgttcga cacgttcgag cgtacgctca cccactatgt tcgcgcgccg
     4021 ttcctgctct cgcaggcagc cgtgcgccac atgcgcgagg caggggccgg atggatcgtc
     4081 aatgtcagct cgcaggatgc gctgcccccc tcgcggccct atcctgatca cgagaaggtg
     4141 cggggctacc acgtctatgc tgcggccaag gcggcactca accgcctgac ccagggcatg
     4201 gccgccgaac tggtcgacga aaacatcgcc gtgaatgtca tcggcccttc gaccgcgatc
     4261 cgcacgcccg gagccgacgc gctgatcccg gcggaattcc cgaccgagga cgtggaatac
     4321 ctggccgaag cggtgctggc catgtgccac ctgccagcgg ccgagcgcac cggcctgatc
     4381 ggctattcga tgcatattcc ctggcactac gggatcccgg tacgcagcct cgatggcaag
     4441 accgaactgc cacggcgcgc gcccccggca tggtcgcacc cggccatcgc ggcatcgggt
     4501 gaataggcgg gccaagctgc aggagagagc tatgacaccc gagcaggcgt ttaccggcgg
     4561 caccgccgtg atcacagggg caggcagcgg cattggcgaa ggcctggccc gcgtggccgc
     4621 ccgtcatggc atgaacgtcg tgcttgccga tatcgcgcag gatcgggtgg aacgggtcgc
     4681 ggcggagatc gccgccaccg gcgcccgggc gctggcggtg gtgaccgacg tgaccgacat
     4741 cgcggcgctg gagcgcttgg ccgatgcggc gtgggatcgc ttcggcggcg tgcgcctggc
     4801 catcaacaat gccgggatcg agacgatcgg ctacagctgg gaactgcccg atgccacctg
     4861 ggacaaggcg atagacatca acattcgcgg ggtggtccac ggctcgcgca tctttgcgcg
     4921 gcgcatgctg gaggccggtc aggcaggcta catcgccaac gtcgcctcga tcgggtcgct
     4981 gggcatgatg ccgacccagg cgccctacat gatgagcaag cacgccgtgc tctcgttcac
     5041 cgaatgcctg ttcctggaaa tgcaggtcag gaacgcgccg atccaggttt cggccgtgct
     5101 gcctgctcag gtcgccacgc gcatattcga agatgccccg gccgacctcg gttcaccgat
     5161 ggtgtcccgc cagcgcgaga tgatgcacgc catgatcacg cagatgggca tcaccccgga
     5221 gcaggccggc gaggttatcc tggccgggat cgcgtcaggg gaattctggg tttcgaccca
     5281 tcccgagacc acggcggcca tggccgcggc gcgcggtgcc tatctggccg cgctcagcac
     5341 gccctcattg cccgaggaag ttcgcgcgct ggttgaatga tgcctgctgg ctgatggcca
     5401 ggcccgatct gggtacctac tggacaggcc atcgctgatg gacaatgatg gcgggcatga
     5461 ccgacgagta cctcgtgggc atgctgttca agcgcctgct tctgttgcgc aacctgcacg
     5521 gcggtccgga ccccgccctt gcccggctgg cgcggggccg ggcccatcga gcatcggaga
     5581 gcacctgaag tggcagcaaa cgatcccatg gtggtcggca tcggccgatc gcactattcg
     5641 cgcaattcgg gccggaccac ctggggcatg gcggtggaat cctgccggct ggcggtggag
     5701 gatgccggca tggccatgtc cgacgtcgac gggatcgtgg agttcaacac ggccgacagc
     5761 accggatcgc tgaacgtggc ctatgggctg gggctgcccg gcatccgcta tccggtggac
     5821 ctgcagaacg gcgggaatat cgtcaccttc gcggtcgccc tggcggcgat ggcggtgcag
     5881 accggcaagt gcaaggcgat cctcgtctac cgttcgctca acagccggtc gggcaagcgg
     5941 ctgggcacat ttaccggtga tctcgcgatc ggcggcgacg accagtacgg cgccccgcac
     6001 ggctatctgg cgcccggcca gtggtttgcc atgtattgcc ggcgctacat gcacgaattc
     6061 ggcacgacca acgaagacct cggcgaggtc gcaatcgtca cccgccagca tgccgtgaag
     6121 aacgactggg cgatgatgcg cgatccgatc accatggctg actacatggc ctcgcgctgg
     6181 atctacgatc cgttccggct ctacgactgc gcgcttgagg cggacggggc ctgcgccatc
     6241 ctcatcacct cccgcgaacg cgcgcaggac ctccggcagg cgcccgtgca ggtggtggac
     6301 gaggagacct tcatgggccg ccatcccgac agctggagcg atttcggcca cctgttctcg
     6361 gcccattcgg cgccgcccct gtggcagcgc accggcctga cagccaggga catggacttc
     6421 gcctgcatct acgactgctt caccttcacc accatcgccg tgatggagga ctatggcttc
     6481 tgcgagaagg gcggggcggg cgaatacttc cgctcgggca aggcgaccta cggcggggac
     6541 t
//
LOCUS       DATNBV010000190         2203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4255429,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000190 DATNBV010000000
VERSION     DATNBV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4255429"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /gene="mrdA"
                     /locus_tag="VN627_04625"
     CDS             <1..1681
                     /gene="mrdA"
                     /locus_tag="VN627_04625"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXD02574.1"
                     /translation="AFSAVTVRMPELPGVIPQRGFSRFYPTGPAVAHLIGYVGPASAE
                     DYEKDRNPLLITPGYKVGKDGLEKAFEPVLRGVPGARRTEVTATGRVVRDLAMREDVP
                     GNPIKLTIDGALQDYAARRIGLESAAVVVVDCQTGGILALVSMPAFDPNSFVGGIGRL
                     QWKMLNEDDHIPLLNKAMRGLYPPGSTMKPMATLALQQHGVDPEERVVCPGGYRLGSR
                     FFRCDAVHGVVNMRSAIEHSCNTYFWSMAHRVGYDAIAPMAKLLGLGQEFDLPGTSQR
                     YGTIPDAAWKMRRYKQEWTVADSLNASIGQGYVSVSPLQLAVMVSRIASGKNLNPSLM
                     FGQEKPLGPALPFTPEQLQVPHDGMFAVVNGGGTAVASKLQVPGIQMAGKTGTAQVRA
                     LTSRGHVGDWKSRDHSLFIGYAPTDAPRYALSVVVEHGTFGARAAAPIARDVLTFLFD
                     QGKAWDTLLAMEKEWGGTPMERMDAKYRAYAAQYGTSAPKVSGDEAMQEAISKADNSA
                     PPVNDAGTTPDTVHEEPPSPSANTPADITARPAQSGAPAPTPSASPAAGAQ"
     gene            1683..>2203
                     /locus_tag="VN627_04630"
     CDS             1683..>2203
                     /locus_tag="VN627_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HXD02575.1"
                     /translation="MLRGTIPDPVARQPWQVILPLFALTTFGATVLYSAAGGSLRPWA
                     LTHFLRFFVFLGMAVAISRVRKETFKSLAYPIYGVIVVLLVLVELVGGIGGGSQRWLN
                     LGFMTLQPSELMKPGIVLVLARFYDDLPASMTASWRALIPAATLLAVPAGLVIIQPDL
                     GTGLAITFGATVVV"
BASE COUNT          330 a          730 c          767 g          376 t
ORIGIN      
        1 tgccttttcc gccgttaccg tgcgcatgcc ggaattgccc ggggtgatcc cgcagcgggg
       61 cttctcccgg ttctatccga ccggaccggc cgtggcccat ctcatcggtt atgtcggccc
      121 ggcctcggcc gaggactacg agaaggaccg caatccgctg ctgatcacgc cgggctacaa
      181 ggttggcaag gacggactgg agaaggcctt cgagccagtc ctgcggggcg tgccgggggc
      241 gcggcgcacc gaggtgacgg ccacgggccg ggtggtccgc gatctcgcca tgcgcgagga
      301 cgttccgggc aacccgatca aattgaccat cgacggcgcg ctgcaggact atgcggcgcg
      361 gcgcatcggc ctcgaatcgg cggctgtcgt cgtggtcgat tgccagaccg ggggcatcct
      421 ggcgctcgtt tccatgccgg ccttcgatcc caactcgttc gttggcggaa tcgggcggtt
      481 gcagtggaag atgctcaacg aggatgacca catcccgctg ctcaacaagg ccatgcgcgg
      541 gctctatccg ccgggttcga cgatgaagcc gatggcgacg ctggccctgc agcagcatgg
      601 cgtggacccg gaggaaaggg tcgtctgccc cggcggctac cggcttggca gccgcttctt
      661 ccgctgcgat gcggtgcacg gcgtggtcaa catgcggtcg gccatcgagc attcgtgcaa
      721 cacctatttc tggtcgatgg cgcaccgggt gggttacgat gcgattgcgc caatggcgaa
      781 actgctgggc ttggggcagg agttcgatct gcccggcacc agccagcgct acggcacgat
      841 cccggacgcg gcctggaaga tgcggcggta caagcaggaa tggaccgtgg cggattcgct
      901 caatgcctcg atcgggcagg gctatgtctc ggtctcgccg ctgcagctgg cggtgatggt
      961 ctcgcgcatt gcctcgggca agaacctgaa cccgtcgctg atgttcgggc aggagaagcc
     1021 cttgggcccc gccctgccat tcacgcccga gcaactgcag gtgccgcatg acgggatgtt
     1081 tgccgtggtc aacggcggcg gcacggcggt cgcctccaag ctgcaggtgc cgggcatcca
     1141 gatggcgggg aagaccggca cggcgcaggt gcgcgcgctg accagtcgcg gccatgtggg
     1201 cgactggaag agccgcgacc attcactgtt catcggctat gcgcccaccg acgcgccgcg
     1261 ctatgcgctc tcggtggtgg tggagcacgg taccttcggc gcccgcgccg ccgcgccgat
     1321 cgcgcgggac gtgctgacct tcctgttcga ccagggcaag gcctgggata ccctgctcgc
     1381 catggaaaag gagtggggcg gcacgccgat ggagcggatg gacgccaagt accgcgccta
     1441 tgccgcgcaa tatggcacct ccgcgcccaa ggtcagcggt gatgaggcga tgcaggaggc
     1501 catttccaag gccgacaaca gcgccccgcc ggtgaacgac gccgggacca cgcccgatac
     1561 ggtgcacgag gagccgccca gcccctcggc caatacaccg gccgatatca ccgcgcggcc
     1621 tgctcaatct ggcgctccag cgcccacgcc gtccgcgtcg cccgcggcgg gagcgcagta
     1681 gcatgctgcg cggcaccatt cccgatccgg tggcgcggca gccgtggcag gtgatcctgc
     1741 cgctgtttgc gctcaccacc ttcggcgcga ccgtgctcta ttccgccgcg ggcggttcgc
     1801 tgcggccctg ggccttgacg catttcctgc gtttcttcgt gttcctgggc atggccgtcg
     1861 cgatctcgcg ggtgcgcaag gagacgttca agagcctcgc ctacccgatc tacggggtga
     1921 tcgtggtgct gctggtgctg gtggagctgg tcggcggaat cggcgggggg agccagcgct
     1981 ggctcaacct ggggttcatg acgctccagc cctcggagct gatgaagccg gggatcgtcc
     2041 ttgtcctggc ccggttctac gacgacctgc cggccagcat gaccgcctcg tggcgggccc
     2101 tcatcccggc ggcgacgctg ctagcggtgc ccgcggggct ggtcatcatc cagcccgatc
     2161 tcggcacggg gcttgccatc accttcggcg cgaccgtcgt cgt
//
LOCUS       DATNBV010000191         2487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3941908,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000191 DATNBV010000000
VERSION     DATNBV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3941908"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VN627_04635"
     CDS             <1..731
                     /locus_tag="VN627_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HXD02576.1"
                     /translation="SDYYGPHVTNRLIREALAPYPDDLVIVTKIGARRGEDGSWLPAF
                     SPEALTQAVHDNLHNLGLDVLEVVNLRIMLDPMAPAEGSIEAPLTALAELRRQGRVRH
                     IGLSNVTPAQVAEGRRICDIACVQNQYNLAHRADDAMIDDLARDGIAYVPFFPLGGFS
                     PLQSSTLSEVAARLGVTPMQVALAWLLRRAPNILGIPGTSSVAHLRENLAAAECVLPD
                     DAVSELDGVARMAGLDKPVAIHQR"
     gene            936..2165
                     /locus_tag="VN627_04640"
     CDS             936..2165
                     /locus_tag="VN627_04640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD02577.1"
                     /translation="MGSKIDSREAWVVAVAALAIASICMGAPLVSSVALKHVAADLGG
                     YRSIPSAALSLAMLGTGVGGLAMGLLAERFGVRPIIMFGATMVCAGLMLSASGEAWQL
                     YLGHGLLIGLLGNAAIHAPLYVYVARWFEARRGTAVALIASGQYVAGAVWPPIFERVV
                     AAYGWRQCMVGFGLFVVAFAVPLAALFLRAPPELVEAKPTAAGAAGNGTVLGLPPNLV
                     FAMLAVASFLCCVPMAMPTAHLIAYCGDIGLSAARGAAMVSVLLVTAFFSRQLWGWTS
                     DRIGGLKTLLVGSAAQVTAMTGFLLTQDEAGLFAVAAAFGLGFSGLVPAYMLAIRQLF
                     PARESSWRIPALLLMAMAGMATGSWSAGVIYDYAGYYAPAFATGIVSNLLHLCIAATL
                     VLLWRRMSARTAVGQVA"
     gene            complement(2219..>2487)
                     /locus_tag="VN627_04645"
     CDS             complement(2219..>2487)
                     /locus_tag="VN627_04645"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HXD02578.1"
                     /translation="FIMNPAVVVVWATGGWLAWAQGMVNDRWLWAKFGLVLLLTGYHH
                     ALGVWRKDFAEDWNTRSPRFYRLMNEVPTLLMIGIVVLVIVKPF"
BASE COUNT          357 a          872 c          826 g          432 t
ORIGIN      
        1 ccagcgacta ctacggcccc cacgtcacca accggctgat ccgcgaggcg cttgcgccct
       61 atcccgacga tctcgtcatt gtcaccaaga tcggcgcccg gcgaggcgag gacggatcgt
      121 ggctgcccgc gttctcgccc gaggctctga cccaggcggt gcacgacaac ctgcacaacc
      181 tcgggctcga cgtgcttgag gtggtcaacc tgcgaatcat gctcgacccg atggcccctg
      241 ccgaaggatc gatcgaggcc ccgctcaccg cactggcaga gctgcggcgg cagggcaggg
      301 tgcgccacat cgggctcagc aacgtaacgc ctgcacaggt cgcggaaggg cgtcggatct
      361 gcgacatcgc ctgcgtgcag aaccagtaca acctggcgca tcgggccgac gatgccatga
      421 tcgacgatct tgcccgcgac ggcattgcct acgtgccgtt cttcccgctc ggagggttca
      481 gcccgctgca atcgtccaca ttgtcggagg tggccgcccg cctcggcgtc acacccatgc
      541 aggtcgcact cgcctggctg ctgcgccgcg cacccaacat ccttgggatc cccggcacct
      601 cgtccgtcgc ccacctgcgg gagaacctcg ccgcagccga atgcgtgctg ccggatgatg
      661 cagtgagcga gcttgacggt gtggcgcgca tggccgggct cgacaaaccg gtcgcgatcc
      721 accaaaggtg acccccgcaa tcggctgggc cgccgggcac gacaatgacc ccggcaaagc
      781 aggtttcccg gcccgcctgg ggcacggcca ggttgtggac cagccgattc cgatggctga
      841 catgcgccgg acccgggcga caaagccttg gcggtcgggc aaattttgtg ctgtcggagc
      901 cggaataatg gtctttactg gtcgggagga ccgcggtggg cagcaaaatc gacagtcggg
      961 aggcgtgggt cgtcgccgtc gcggcgctgg cgattgcctc catctgtatg ggcgcgcccc
     1021 tggtgtccag cgtggccctc aagcacgtgg cggccgatct cggcggttat cgctccatcc
     1081 cctcggcggc cctctccctc gccatgctcg gcaccggcgt cggcgggctg gcgatgggct
     1141 tgctggccga gcgcttcggc gtgcgcccga tcatcatgtt tggcgccacg atggtgtgcg
     1201 ccggcctgat gctctcggcc agcggtgaag cctggcagct ctatcttggg cacggcctcc
     1261 tcatcggtct gcttggcaac gcagccatcc atgcgccgct ctacgtctat gtcgcccgct
     1321 ggttcgaggc gcggcgcggc acggcggtgg cgctcatcgc cagcggccag tatgtggcag
     1381 gtgcagtctg gccgccgatc ttcgagcgcg tggtcgccgc ctacggctgg cggcaatgca
     1441 tggtcggctt cggcctcttc gtcgtggcgt ttgccgtccc gttggcggcg ctgttcctgc
     1501 gcgcgccgcc agagctggtg gaggccaagc cgaccgcggc cggcgcggcc ggcaacggca
     1561 ccgtcctcgg cctgccgccg aacctcgtat ttgccatgct cgccgttgcc tcgttcctct
     1621 gctgcgtgcc catggcgatg ccgaccgccc acctgatcgc ttactgtggc gatatcggcc
     1681 tcagcgcggc caggggcgcc gccatggtgt ccgtgctgct ggtgacggct ttcttcagcc
     1741 ggcagctgtg gggctggacg tccgaccgta tcggcggtct caagacgctc ctcgtcggct
     1801 cggcggcgca ggtcacggcc atgacgggct tcctgctgac gcaggacgag gccggcctgt
     1861 ttgccgtggc ggcggcattc ggcctcggct tctcggggct ggtccccgcc tacatgctcg
     1921 ccatccgcca gctgtttccg gcgcgcgagt ccagctggcg catcccggcc ctgctgctga
     1981 tggcgatggc cggcatggca acggggagct ggagcgccgg cgtcatctac gactacgccg
     2041 gctactacgc ccccgccttc gccaccggca tcgtttccaa cctgctccac ctctgtatcg
     2101 ccgccaccct ggtattgctc tggcgacgga tgtcggcccg caccgcggtg ggacaggtgg
     2161 catagcgggc ggcctgacgg gccttcaggg aggggcgaca ggcagggcgc aatcggccct
     2221 agaacggctt gacgatcacc agcaccacga tcccgatcat gagaagcgtc ggcacctcgt
     2281 tcatgaggcg gtagaagcgc gggctgcggg tgttccagtc ctcggcgaag tctttgcgcc
     2341 agacgccaag cgcgtggtgg tagccggtca gcagcagcac caggccgaat ttggcccaca
     2401 gccagcgatc gttgaccatg ccctgcgccc aggccagcca gccgcccgtc gcccacacca
     2461 ccacaaccgc tggattcatg atgaagg
//
LOCUS       DATNBV010000192         7242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4591145,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000192 DATNBV010000000
VERSION     DATNBV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4591145"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VN627_04650"
     CDS             complement(<1..621)
                     /locus_tag="VN627_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXD02579.1"
                     /translation="MKRRKSDSSRGRIAAKIPTETKLVKSAGRVLEILEFFDDFQRPA
                     TVMEVAVALSYPQSSTSALLRSLVATGYLTYDAQARTYITSTRVALLGSWLNSPFFAE
                     GTLISLMKELNELTGDTVVLATRNGLYAQYIHVIQATSPARLHLTLGTVRPLAASGAG
                     YAILSTMTDAEITRIVMRMNAEAPEGQPLVKLRDLLADIEQVRERGY"
     gene            975..2135
                     /locus_tag="VN627_04655"
     CDS             975..2135
                     /locus_tag="VN627_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD02580.1"
                     /translation="MSAARLHLELPVLTPEAEELRRDVRAFIAEERDSGRLPPPRRIG
                     LGFDKAMTRRIAAKGWIGLTWPKQYGGQERSALERYVLNEELLAAAVPVGAHWVADRQ
                     SGPVLLKFGTEAQRQFYLPRIAAGELTFSIGMSEPESGSDLASLKTKAEKVDGGWKIN
                     GRKIWTTNAHHAQYIIALCRTSVVEGSRHDGLSQLIVPMDSPGVSISPIVSMALEHDF
                     NEVLFEDVFVPDDALIGKEGNGWNQVSAELAYERSGPERWLSSFRLISELVDVLGPDA
                     RPSTLEDLGRLIGQLLSLRQLSMSVASMLQQGKSPNMEASIVKDLGTKFEQEAVRVVR
                     DIVNAEDFISHGRAERLQALMIHAQQYAPGFTIRGGTNEILRGIIARGIGLR"
     gene            2140..3198
                     /locus_tag="VN627_04660"
     CDS             2140..3198
                     /locus_tag="VN627_04660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD02581.1"
                     /translation="MSEQMNENRAFFRDTVERILADTLDKSHIDAAENGELPDALYGA
                     LVENGITLMLVPEDQGGIGASLGDAAVILRAAGEAAAPGPLLETMLGQALLASAGLEP
                     ADGLVTLAFVDEFEAPDVGASTWANAAVHDIPWAGKVGKVLVVARAAGNAARLVVSDA
                     AAWTVATRPDAAGEPRDSISGAAVPVMVAEIGDYDHWLRSAAILRGAQILGAIDWSFR
                     RSVEYAGERKQFGREIGKFQAIQQMLAELAGHVLAASGIVEAAAEGATPALVAAARSR
                     LGDAGDAAITICHQVHGAMGFSLEYALNSRTRRIMAWRNDFGSVPYWRRTLAGGFAGL
                     SREGFWPAVADAGLQQVA"
     gene            3200..3754
                     /locus_tag="VN627_04665"
     CDS             3200..3754
                     /locus_tag="VN627_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD02582.1"
                     /translation="MSAPADWTPERLADRGQITEVLYRYCRAIDRIQAGHLIGPVFHE
                     DALIDKTGTPDPVAAWAAVVAQRHPGVPAASHMVTNVLIEFLGPDSAFVESWCLAVER
                     HPPTADEPRTMDRVYRVRYADRFERRADGLWRIASRTFLTDHVMAIPLDSAPDPLGAS
                     RLTTRRDADDPAMRMRRELGLPPV"
     gene            3835..4500
                     /gene="hisG"
                     /locus_tag="VN627_04670"
     CDS             3835..4500
                     /gene="hisG"
                     /locus_tag="VN627_04670"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HXD02583.1"
                     /translation="MLAEKKPLTFAVPKGRILDEALPLMERAGVVPVAEFHDKKSRAL
                     SFACENSDMRIIRVRAFDVATFVAHGAAQVGIVGSDVVEEFAYADLYAPVDLDIGHCR
                     LSVAEPAGEATRNGAASHLRVASKYPNLTRKHFEKMGVQAEVVKLNGAMELAPGLGLA
                     SRIVDLVSTGKTLKDNGLVETSIILPVSARLIVNRAALKTDERVGALVEQFRAAVASL
                     KAA"
     gene            4500..5792
                     /gene="hisD"
                     /locus_tag="VN627_04675"
     CDS             4500..5792
                     /gene="hisD"
                     /locus_tag="VN627_04675"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXD02584.1"
                     /translation="MLRLSTRDSGFEKAFRRLVRDRRESEADVARDVELILADVRNRG
                     DAALAELTAKFDRHQLVSDDDWRIAPDACAAAYEALDSELRTALDLAAERIRAYHAAQ
                     KPENRDYTDATGVRLGAVWRAVDAAGLYVPGGRAAYPSSLLMNALPAKVAGVERLVVV
                     TPTPGGAANPLVLAAAHIAGVDEVWRVGGAHAVGALAYGTDRIRPVDVITGPGNAWVA
                     EAKRQLYGVVGIDMVAGPSEILVIADAKNNPEWIAADLLSQAEHDPAAQAILITDDPV
                     FADMVADQVGVTLSLLRTAKVASESWNTHGVIIEVGSLDEAPALANALAAEHVEIATD
                     EPEALFRAIRHAGSVFLGRHTPEAIGDYLAGPNHVLPTGRRARFASGLSVLDFMKRTS
                     FIQMSEGAMGAIGPAAVALAEAEGLPAHARSVAVRLGQ"
     gene            5789..6232
                     /gene="nusB"
                     /locus_tag="VN627_04680"
     CDS             5789..6232
                     /gene="nusB"
                     /locus_tag="VN627_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXD02585.1"
                     /translation="MKSGSKARAAARLAAVQALYQYEMEETRLPQLLDEFHMHRLGAE
                     IEDDQYAQADVRFFDDLVKGAIARRDEIDGLLAEKLATGWKIERLDRTMLQILRAGAY
                     ELMARADVPTGTAISEYLDVAHAFFDEREAKFVNGVLDAVARAVR"
     gene            6386..>7242
                     /gene="thiL"
                     /locus_tag="VN627_04685"
     CDS             6386..>7242
                     /gene="thiL"
                     /locus_tag="VN627_04685"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HXD02586.1"
                     /translation="MTTELAFIAALRGLATHPAARNLDDDCAVLEIGGEALVLTHDVM
                     AEGVHFLPEADPADVAWKLVATNMSDLAAKGAEPFGVLLGYQLGKDDTRFLTGLGDAL
                     THYGAALLGGDTVGGNGPQTVGLTAIGRATTRPVPSRAGARAGDRVWLTGAVGGAMIG
                     LEALRAGAGDSTAYRRPKARLAEGISLARRVTAMMDVSDGLLLDAWRLARASGVSLAL
                     NTAAVPLACPESRRHEALRWGDDYELLFTLPAATDPGVGATCIGEVQAGPAGTLRLDG
                     RLVESGENLG"
BASE COUNT         1178 a         2562 c         2385 g         1117 t
ORIGIN      
        1 ataaccgcgc tcgcgcacct gctcgatatc ggcaaggaga tcgcgcagct tgaccagcgg
       61 ctgcccctcg ggcgcctcgg cgttcattcg catgacgatg cgggtgatct cggcatccgt
      121 catggtcgaa aggatcgcat agcccgcccc cgaggcggcg agcgggcgga ccgtgccgag
      181 ggtcaggtgg agccgcgccg ggctggttgc ctggatcacg tggatatatt gcgcgtaaag
      241 cccgttgcgg gtcgccagca caaccgtgtc gccggtgagc tcgttgagct ccttcatcag
      301 cgagatgagc gtcccttcgg caaagaacgg cgaattgagc cacgaaccga gcagggcgac
      361 gcgggtcgag gtgatatagg tgcgggcctg ggcatcatag gtgagatagc cggtggcgac
      421 gaggctgcgc agcagggccg aggtgctgga ctgcggatag gacagcgcca ccgccacttc
      481 catcactgtc gccgggcgct ggaaatcgtc aaagaactca aggatctcga gcacgcgacc
      541 ggcggacttg accagtttgg tctcagtcgg gatcttggcg gcaatccggc cgcgggaaga
      601 atcggatttg cgacgtttca tggcatcaca tcctctctgc cgacccagtt cgccatcgcc
      661 agagcgatga aatcgaatcg gtcgatcaca ttctcgatgt agaggcgcct agtcccaacc
      721 ggtaagagcc tcaactgttt tgccccccag caggtgcaaa tcccgaaaat cacccgaatt
      781 cgatccgcta tatcacatat atgcgtcggt tttcccggaa tcacaggtag cgggcaacag
      841 aagcgtgatg gatagagcga tcgacattgc accggccgca accctcccca taggcatcgg
      901 ccattctccg acagctcctc cagaggaacc cagatgatcg ttcgcacagg gcacggcccg
      961 acgggagccc ggccatgagt gccgcccgcc tccacctgga attgcccgtc ctcaccccgg
     1021 aagccgaaga gctgcgccgg gacgtccgcg ccttcatcgc cgaggagcgg gacagcggcc
     1081 gcttgccgcc gccgcgccga atcggcctgg gattcgacaa ggcaatgacc cgccgcatcg
     1141 cggccaaggg ctggatcggc cttacctggc ccaagcaata tggtggtcag gaacgctccg
     1201 cgctcgaacg ctatgtgctc aacgaagagc tgctggccgc cgccgtgccg gtcggcgcgc
     1261 actgggtggc cgatcgccag tcgggtccgg tcctgctcaa gttcggcacc gaagcccagc
     1321 gccagttcta cctcccccgg atcgctgcgg gcgagctgac cttctcgatc ggcatgagcg
     1381 aaccggaatc cggctccgac ctcgcctcgc tcaagaccaa ggcggagaag gtcgacggcg
     1441 gctggaagat caacggccgc aagatctgga cgaccaacgc ccaccacgcc cagtacatca
     1501 tcgcgctgtg ccggacctcg gtggtcgagg gttcgcgtca cgatggcctg agccagctga
     1561 tcgtgccgat ggacagcccg ggcgtgtcga tcagcccgat cgtatccatg gcgctcgagc
     1621 atgatttcaa cgaagtcctg ttcgaggacg tgttcgttcc cgacgatgcc ctgatcggca
     1681 aggaaggtaa cggctggaac caggtctcgg ccgaactggc ctatgagcga tccggcccgg
     1741 aacggtggct ctccagcttc cgcctgatca gcgagctcgt cgacgtgctc ggccccgatg
     1801 cccgcccctc cacgctcgag gatctcggcc ggctgatcgg ccagctgctc tccctgcgcc
     1861 agttgtcgat gtcggtcgcc tcgatgctcc agcagggcaa gtcgcccaac atggaggcct
     1921 cgatcgtcaa ggacctcggc accaagttcg agcaggaagc cgtccgcgtc gtgcgcgaca
     1981 tcgtcaatgc cgaggatttc atttcccatg gccgggccga gcgccttcag gccctgatga
     2041 tccacgccca gcaatatgcg cccggcttca ccatccgtgg cggcaccaac gaaatcctgc
     2101 gcggcatcat cgcccgcggg atcggcctga ggtaaggcga tgagcgaaca gatgaacgag
     2161 aaccgcgcct tcttccgcga caccgtggaa cgcattctgg ccgatacgct ggacaagtcg
     2221 cacatcgatg ccgccgagaa cggcgagcta cctgacgcgc tgtatggcgc gctcgtggag
     2281 aacggcatta ccctgatgct ggtgcccgag gaccagggcg ggatcggcgc cagcctcggc
     2341 gatgccgcag tcatcctgcg cgccgcgggc gaagcggccg cccctggccc gctgctcgaa
     2401 accatgctcg gccaggccct gctcgccagc gcggggcttg agcccgccga cgggctggtg
     2461 acccttgcct ttgtcgacga attcgaagct cccgatgtcg gcgccagcac gtgggccaac
     2521 gccgccgtgc acgacattcc atgggccgga aaggtcggca aggtgctggt tgtcgcccgc
     2581 gctgccggca acgccgcccg cctcgtcgtc agcgacgccg ccgcatggac cgtggcgaca
     2641 cgccccgacg ccgccggcga accgcgcgac tccatttccg gcgcggccgt cccggtaatg
     2701 gtggccgaga ttggcgacta cgatcactgg ctgcgctcgg ccgcgatcct gcgcggcgcc
     2761 cagattctcg gcgcgatcga ctggtcgttc cgccgcagcg tcgaatatgc cggcgaacgc
     2821 aagcagttcg ggcgcgaaat cggcaagttc caggcaatcc agcagatgct ggctgaactg
     2881 gctggccacg tcctcgcggc cagcgggatc gtcgaggccg cggccgaggg agctaccccc
     2941 gcgctggtcg cggcggcgcg ctcgcgcctt ggtgatgccg gcgatgccgc gatcacgatc
     3001 tgccaccagg tccacggcgc catgggcttc agcctcgaat atgcgctgaa ctcgcggacc
     3061 cggcggatca tggcctggcg caatgacttc ggcagcgtgc cctactggcg gcgcaccctg
     3121 gccggcggct tcgccgggct gtcgcgcgaa ggcttctggc ccgccgtggc cgacgccggc
     3181 ctgcagcagg tcgcctgacg tgagcgcccc ggccgactgg acgcccgagc gcctggccga
     3241 ccgcggccag atcaccgaag tgctctaccg ctactgccgc gccatcgacc gcatccaggc
     3301 cggccacctg atcggcccgg tgttccatga ggacgcgctg atcgacaaga ccggcacccc
     3361 cgatcccgtg gccgcctggg ccgccgtggt cgcccagcgc caccccggcg ttccggcagc
     3421 ctcgcacatg gtcaccaacg tactgatcga gttcctcggg ccggacagcg ccttcgtaga
     3481 gtcatggtgc ctcgccgtcg agcgccatcc gcccacggca gacgagccgc gcaccatgga
     3541 ccgggtctac cgcgtccgct acgccgaccg cttcgagcgc cgcgccgatg gcctctggcg
     3601 catcgcctcg cgcaccttcc tgaccgacca cgtcatggcc atcccgctcg acagcgcgcc
     3661 cgacccgctc ggcgccagcc ggctgacgac ccggcgcgat gccgatgatc cggccatgcg
     3721 gatgcgccgc gaactggggc tgccgcccgt ctgaccgcag gggtgtgccg tggcaatgcg
     3781 gcgcgggcag ccatttcctt gcccttccgc cgatctgccc ctagaggcgc ggccatgctt
     3841 gccgagaaga agcccctgac gttcgcggtc cccaagggcc gcatcctcga cgaggcgctc
     3901 ccgctgatgg aacgcgccgg tgtggtgccc gttgcggagt tccacgacaa gaagtcgcgc
     3961 gcgctgtcct tcgcctgcga gaacagcgac atgcggatca tccgcgtgcg cgccttcgac
     4021 gtggcgacct tcgtggccca cggcgcggcc caggtcggca tcgtcggctc cgacgtggtc
     4081 gaggaattcg cctatgcgga cctttacgct cccgtcgatc tcgatatcgg ccactgccgc
     4141 ctgtccgtcg ccgaaccggc cggggaagcg acgcgcaacg gtgctgcgag ccacctgcgc
     4201 gtggccagca agtatcccaa cctcacccgc aagcacttcg agaagatggg cgtgcaggct
     4261 gaggtggtga aactcaacgg tgcgatggaa ctcgccccgg gcctgggcct cgccagccgg
     4321 atcgtcgacc tcgtgtccac cggcaagacc ctcaaggaca atggcctggt cgagaccagc
     4381 attatcctcc ccgtctccgc ccgcctgatc gtcaaccgcg cggcgctgaa gacggatgag
     4441 cgcgtcggcg ctctggtgga gcagttccgg gcagccgtcg cctcgctgaa ggcggcctga
     4501 tgctgcggct gagcacccgc gattccggct tcgagaaggc tttccgccgc ctcgtgcgcg
     4561 atcggcgcga gagcgaggcc gatgtcgcgc gcgatgtcga gctgatcctc gccgacgtcc
     4621 gcaaccgcgg cgatgcggcc ctcgcggagc tcaccgccaa attcgaccgc caccagttgg
     4681 tgagcgacga tgactggcgc atcgcgcccg acgcctgcgc cgcggcctat gaggcactgg
     4741 acagcgaact gcgcaccgcg ctcgatctcg ccgccgagcg catccgcgcc taccacgccg
     4801 cgcagaagcc ggaaaaccgg gactatactg acgccaccgg agtgcgcctc ggcgcggtct
     4861 ggcgggcggt tgacgcggcc gggctctacg tccccggcgg gcgtgcggcc tatccctcgt
     4921 cgctgctgat gaatgccctc ccggctaagg tcgccggggt cgagcggctg gtcgtggtga
     4981 cgccgacgcc cggcggggcg gccaacccgc tggttctcgc cgccgcccac atcgccggcg
     5041 tcgacgaggt ctggcgcgtc ggcggggccc atgcggtcgg cgccctggcc tatggcaccg
     5101 accggatcag gccggtcgac gtgatcaccg gcccgggcaa tgcctgggtc gccgaggcca
     5161 agcgccagct ctacggcgtc gtcggcatcg acatggtcgc gggccccagc gaaatcctcg
     5221 tcatcgccga cgcgaagaac aaccccgaat ggattgccgc cgacctgctc agccaggccg
     5281 agcacgaccc ggcggcccag gccatcctca tcaccgacga tccggtcttc gccgacatgg
     5341 tcgccgatca ggtcggagtg accctttcgc tcctgcgcac ggccaaggtg gccagcgaga
     5401 gctggaacac gcacggcgtg atcattgagg tcggcagcct tgacgaggcc ccggccctgg
     5461 ccaacgccct cgccgccgaa cacgtggaga tcgccacgga cgagccggag gccctgttcc
     5521 gggcgatccg ccacgccggc tcggtcttcc tcggccgcca cacgcccgag gccatcggcg
     5581 actatctggc cgggccgaac cacgtgctgc ccacgggccg ccgcgcgcgg ttcgcctccg
     5641 gtctctcggt gctcgatttc atgaagcgaa ccagcttcat ccagatgagc gaaggcgcca
     5701 tgggcgcgat cgggcccgcc gccgtggcgc tggccgaagc ggaaggcctg ccggcgcatg
     5761 cgcggtcggt ggcggtgagg ttgggtcaat gaagtcgggt tcgaaagcac gcgccgccgc
     5821 acggctggcc gccgtccagg cgctctatca gtacgagatg gaagaaacgc gcctgcccca
     5881 gctgctcgac gaattccaca tgcaccgcct tggcgccgag atcgaggacg accagtacgc
     5941 ccaggccgac gtgcgcttct tcgacgacct cgtgaagggc gccattgccc gccgcgatga
     6001 gatcgacggc ctgctggccg aaaagctcgc caccggctgg aagatcgagc ggctagaccg
     6061 caccatgctc cagatcctgc gcgccggcgc ctacgaactc atggcgcggg cggatgtgcc
     6121 caccggcacc gcgatcagcg aatacctcga tgtggcccac gccttcttcg atgaacgcga
     6181 ggcgaagttc gtgaacgggg tgctggacgc cgtggccagg gctgtgcggt gacatgaagg
     6241 tcctccccgt tttcgtcgca ggcgcaaagg gggaggtggc aggcgccgtc aggcgtgtgg
     6301 cggaggggtt gcagccgctc caccaaccgc tgcgcgggcg gtccccatcc ccccttgtgc
     6361 cttcggcaag agcggggagg atctggtgac caccgaactt gccttcatcg ccgccctgcg
     6421 cggcctcgcc acccatcccg ccgcgcgcaa tctcgacgac gattgcgccg tgctggagat
     6481 cggcggcgag gcgctggtgt tgacgcatga cgtcatggcc gaaggcgtcc atttccttcc
     6541 cgaggccgat ccggcggacg tggcgtggaa gctggtcgca accaacatgt ccgatctcgc
     6601 agccaagggc gccgagccct tcggcgtgct gctgggttac cagctgggca aggacgatac
     6661 ccggttcctg accggcctcg gcgacgccct cactcactac ggcgccgcct tgcttggcgg
     6721 cgacaccgtg ggcggcaacg gtccgcaaac cgttgggctg accgccatcg gccgggccac
     6781 cacccgcccc gtgccctccc gcgctggcgc ccgggccggt gaccgggtat ggctgacggg
     6841 tgccgttggc ggagcgatga tcgggctcga agccttgcgc gccggagcgg gcgacagcac
     6901 ggcttaccgc cgccccaagg cgcgccttgc cgaagggatc agcctcgccc ggcgcgtcac
     6961 cgccatgatg gacgtttccg acgggctcct gctcgatgcc tggcggcttg cccgggcaag
     7021 cggggtgagc ctcgccctga acacggccgc agtccccctg gcctgccccg aatcgcgccg
     7081 ccacgaggca ctgcgctggg gggatgacta cgaactgctg ttcaccttgc ccgctgcaac
     7141 cgatcccggc gtgggggcaa cctgcatcgg cgaagtgcag gccggcccag cgggaaccct
     7201 gcgcctcgat ggccgactgg tcgaatccgg ggaaaatctc gg
//
LOCUS       DATNBV010000193         2340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2335240,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000193 DATNBV010000000
VERSION     DATNBV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2335240"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VN627_04690"
     CDS             <1..964
                     /locus_tag="VN627_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HXD02587.1"
                     /translation="NAGVGQSLANATTMALLDTNAENLRITTYDTAGGSSAAATRAIA
                     DGNKLILGPLMGDEVAAVAHVARPARVPIISFSNDDSVAAPGAFIMGSLPAQSIARTV
                     RYARAHGANRFAALVPEGEYGQRAEAAFKSAVIGSGGTIAGIESYARTNTSVISAARR
                     LKAKGGFDAVLLADNGRLAALAAPSLRGGKAPVRLLGTELWSADPVVTGSPALSGALY
                     SAVSEGRFRQFAESYKNRFGTPPHRIATLGYDAVLLTLRVAREWRPGTTFPMARIGDR
                     GGFLGLDGPFRFGEDGVVQRAFEVNEVRPGGVTVVSPAPARFQD"
     gene            1030..1881
                     /locus_tag="VN627_04695"
     CDS             1030..1881
                     /locus_tag="VN627_04695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXD02588.1"
                     /translation="MALPRISIITPAYRAEQFIESTITSVVSQGYPDLEYIVLDGAGD
                     STADILRRHDESIHHWRSAPDEGQYAAVTEGFAMATGEVLGWLNADDILLPRSLFVIG
                     EIFAQFPEIDWITTNKPGLMDASGALAHFGYVPGFNARAFLDGFYLQGAAPKGFFIQQ
                     EATFFRRSLWEKAGACIPPFPLAGDFALWCEFYKHAELVSVAYPLAAFRVVEGQRSEA
                     IDAYLAEGREALANLRQAVNYAPSAPFPENPVYPSRVIMNQNPRQVGAGWTIVPYNYA
                     PMTDISL"
BASE COUNT          395 a          864 c          689 g          392 t
ORIGIN      
        1 caatgccggg gtcgggcaaa gcctcgccaa tgcaacgacc atggccctgc ttgataccaa
       61 cgccgaaaac ctgcggatca cgacctatga cactgccggc ggctcgtccg ccgcggccac
      121 ccgcgccatc gccgatggca acaagctgat cctcgggccc ctcatgggag acgaggtcgc
      181 cgccgtggcc cacgtcgccc ggcccgcccg cgtgccgatc atctcgtttt cgaacgacga
      241 tagcgtggcc gcgcccggcg cctttatcat gggcagcctt ccggcccagt cgatcgcccg
      301 caccgtgcgc tatgcccgcg cccacggcgc caatcgcttc gccgcgctcg tccccgaagg
      361 cgaatatggc cagcgcgccg aagccgcctt caagagcgcc gtgatcggat ccggcggcac
      421 catcgccggc atcgaaagct atgcccgcac caacacctcg gtgatcagcg ccgcccgccg
      481 gctcaaggcc aagggcgggt tcgacgcggt cctgctggcc gacaacggcc ggctcgccgc
      541 cctcgccgca ccgtccctgc gcggcggcaa ggccccggtc cggctgctcg gcaccgaact
      601 gtggagcgcc gatccagtgg tgaccggttc gcccgccctg tccggcgcgc tctattcggc
      661 cgtgtcggaa gggcgcttcc gccagttcgc cgaaagctac aagaaccgct tcggcacccc
      721 gccccaccgc atcgccaccc tcggctacga cgcggtcctg ctgaccctgc gcgtcgcgcg
      781 cgagtggagg ccgggaacca ccttccccat ggcccgcatc ggcgatcggg gcggcttcct
      841 cggtctcgat ggccccttcc gctttggcga agatggcgtc gtccagcgcg ccttcgaggt
      901 gaacgaggtt cgccccggcg gtgtcaccgt ggtcagtccc gcaccggcgc gcttccagga
      961 ctgagcaggg ccgacagggg tcgacatcgg tggccgcatc gttaacctca atcgtggaag
     1021 ctcgaattaa tggcattgcc gcgtatttcc atcatcaccc cggcctaccg ggctgaacaa
     1081 ttcatagaat cgacaataac ttcggtcgtc tcacaaggtt atcccgatct tgaatacatc
     1141 gtgctcgacg gcgccggcga ttcgaccgcc gatatcctgc gccgccacga tgagagcatc
     1201 catcactggc gcagcgcgcc cgacgaaggc caatatgcgg ccgtaaccga agggttcgcc
     1261 atggccaccg gcgaggtgct gggctggctg aacgccgacg atatcctgct gccccggtcg
     1321 ctgttcgtga tcggcgaaat cttcgcgcag ttcccggaga ttgactggat caccaccaac
     1381 aagcccgggc tcatggacgc cagcggtgcc ctggcccatt tcggctatgt gccgggtttc
     1441 aacgcgcggg ccttccttga tgggttctac ctgcagggcg cggcgcccaa gggcttcttc
     1501 atccagcagg aggcaacctt cttccgccgg agtctgtggg agaaggctgg ggcctgcatc
     1561 cccccatttc cgctcgccgg cgatttcgcc ctgtggtgcg agttctacaa gcacgccgaa
     1621 ctcgtgtccg tcgcttatcc gctggccgcc ttccgcgtgg tcgagggaca aaggtcggaa
     1681 gcgatcgacg cctatctagc ggaagggcgg gaggcgctgg cgaaccttcg ccaggcggtc
     1741 aactacgccc cctccgcgcc attcccggaa aatccggtct atccttcccg ggtcatcatg
     1801 aaccagaacc cccgccaggt cggcgccggc tggaccatcg ttccctacaa ctacgccccc
     1861 atgaccgaca taagcctgta accggcgctt gcggacaacc ccgtcccgcc ccgggcatgg
     1921 tcgaaggcga tccagaagca aatcccgccg ctcctcgcta acgcccccgg gaaaagaaac
     1981 ccgtcgcccc tgcgaaggca ggggtccatc ccagcctttc gggtctgccc agacaatgcg
     2041 ttgatcagca aacctcgcct gggcccctcg ccccgtgtcg ctgtgaggcc aagatggccc
     2101 cacacctgac gataaccccc aacccccgcc ttgcgcccgg aatcccgccg ctcctataac
     2161 cggtcctatg tccgacgatc tcttcgacaa gctccccgcc gccagcggcg aagcctatga
     2221 tggttccgcg atcgaggtgc tcgaagggct tgagccggtc cgccgccgtc cgggcatgta
     2281 tatcggcggc acggacgaac gcgctttcca tcacctcgct gccgaagtgc tcgacaatgc
//
LOCUS       DATNBV010000194         2261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3304799,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000194 DATNBV010000000
VERSION     DATNBV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3304799"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VN627_04700"
     CDS             complement(<1..329)
                     /locus_tag="VN627_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HXD02589.1"
                     /translation="MTPQARVQAGIEILDAVIAAARNKGAPADRIIADWFRPRRYAGS
                     KDRRAVRELVYAAIRQCGEVPVSGRAAMLRVAEGDPALASLFDGSRHAPAPVVPGEPV
                     ASGGTAPG"
     gene            complement(339..1796)
                     /gene="guaB"
                     /locus_tag="VN627_04705"
     CDS             complement(339..1796)
                     /gene="guaB"
                     /locus_tag="VN627_04705"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HXD02590.1"
                     /translation="MDIPLGLTFDDVLLRPAESDILPSMADTRTKLTREIALNIPVIS
                     SAMDTVTEADMAIVMAQLGGIGVLHRNLTVEEQCAAVRAVKRFESGMVVNPITISPNA
                     TLGEARALMQVNRISGIPVVESNGKLCGILTNRDVRFADNPAQPVRELMTHQNLATVK
                     TGVSQEEARRLLHQRRIEKLLVVDEAYHCVGLITVKDIEKAVNYPSATKDAAGRLRVA
                     AATTVGDKGFERTEALLAAECDVVIIDTAHGHNRDVARAVERVKKLSNSAQVVAGNVA
                     TAEATRALIDAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIMDAAEAADKLGVPVIG
                     DGGLRTSGDAAKALAAGASVVMVGSMLAGTEEAPGETFLYQGRAYKSYRGMGSVGAMA
                     RGSADRYFQQDIKDQMKLVPEGIEGQVPYKGPAKDVIHQLVGGIKAAMGYTGCGTIDE
                     LHSKAQFVQITGAGLKESHVHDVTITREAPNYKVG"
BASE COUNT          357 a          796 c          715 g          393 t
ORIGIN      
        1 ccgggcgcgg tgccgccgct ggcgaccggc tctcctggca ccaccggcgc aggagcgtgg
       61 cgcgaaccat cgaataggct ggcaagcgcc gggtcaccct cggccacccg cagcatggcc
      121 gcccgcccgc tcaccggcac ctcaccgcac tgccgaatcg ccgcatagac cagttcgcgc
      181 acggcgcggc ggtccttgga accggcatag cggcgcgggc ggaaccagtc ggcaatgatc
      241 cggtcggccg gcgcgccctt gttgcgcgcg gccgcaatca ccgcatcgag tatctcgatg
      301 cctgcctgaa cccgggcctg gggggtcatg ccccggcctc agccgacttt gtagttcggc
      361 gcttcccgag tgatcgtcac gtcgtggacg tggctttcct tgagccccgc gccggtgatc
      421 tggacaaact gggccttgct gtgcagctcg tcgatcgtgc cgcagccggt atagcccatc
      481 gccgccttga tcccgccgac cagctggtgg atcacatcct tggccggccc cttgtacggc
      541 acctgcccct cgatcccctc gggcaccagc ttcatctggt ccttgatgtc ctgctggaag
      601 tagcgatccg ccgagccgcg cgccatggcg ccgaccgaac ccatgccgcg gtacgacttg
      661 taggcccgcc cctggtagag gaacgtctcg cccggcgctt cctcggtccc ggccagcatc
      721 gagccgacca tcaccaccga agctccggcc gccaaggcct tggccgcgtc gcccgaggtg
      781 cgcaggccgc cgtcgccgat caccggcacg cccagcttgt ccgcggcttc cgcagcgtcc
      841 attatcgcgg tcagctgcgg cacgccaacg cccgcgacga tccgggtcgt gcagatcgag
      901 cccggcccga tccccacctt gaccgcgtca gcccccgcat cgatcagcgc gcgggtggcc
      961 tcggccgtgg caacgttgcc cgccacgacc tgcgccgaat tcgacagctt cttcactcgt
     1021 tccaccgcac gggcgacgtc gcggttatgg ccatgggcgg tgtcgatgat caccacatcg
     1081 cactcggcgg ccagcagggc ctcggtgcgc tcaaagccct tgtcgcccac cgtggtcgcg
     1141 gcggcgacgc gcaggcgccc ggcggcatcc ttggtggcgg acgggtagtt gaccgccttc
     1201 tcgatgtcct tgaccgtgat caggcccacg caatgataag cctcgtcaac caccagcagc
     1261 ttctcgatcc gccgctggtg cagcagcctg cgggcttctt cctgcgaaac gccggtcttc
     1321 accgtggcga ggttctggtg cgtcatcagc tcgcgcacgg gctgggccgg gttatcggca
     1381 aagcggacat cgcggttggt gagaatgccg cacagcttgc cattggattc gaccaccggg
     1441 atgccggaaa tgcggttcac ctgcatcagc gcccgggctt cgcccagggt ggcgttgggc
     1501 gagatcgtga tcgggttcac caccatcccg ctctcgaacc gcttgactgc gcggacggcg
     1561 gcacactgct cttccacggt gaggttgcgg tgaagcaccc cgatcccccc gagctgtgcc
     1621 atgacaatcg ccatgtcggc ctcagtcacc gtgtccatcg ccgaggagat caccgggatg
     1681 ttgagcgcga tctcgcgggt cagcttcgtc cgggtatcgg ccatcgaagg caggatgtcg
     1741 gactctgccg ggcgcagcag cacgtcatcg aaggtaaggc cgagggggat gtcgatcagt
     1801 gccactggtt tgcctgtctg ctcgggaagt tcccggggga ttcgtggcgg cccatgtaac
     1861 cgcatgggtg cgggttaaca agccaaggtt cccggcgcat gacagcggcg agatttcacg
     1921 cggaggcgcg gagaagaata agggttcgcg cagagatcgc agaggacgca gagacgatgg
     1981 gcctttccgc gaagcggaga cgaaacctag atgaggctct gagcgcacgg acattggagg
     2041 cagcttcgcc gcccaagcct ctctgcgtcc tctgcgatct ctgcgcgaac gattccctgt
     2101 tcttctccgc gcctccgcgt gaaatctcgc cgctgtcatg cgctgggaac cttggcttgt
     2161 taacccgcac ccatgcggtt acatgggccg ccacgaatcc cccgggaact tcccgagcag
     2221 acaggcaaac cagtggcact gatcgacatc cccctcggcc t
//
LOCUS       DATNBV010000195        10759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1382391,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000195 DATNBV010000000
VERSION     DATNBV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382391"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VN627_04710"
     CDS             <1..185
                     /locus_tag="VN627_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HXD02591.1"
                     /translation="TGSIMGGATVHDVERFVAMYMAGEITLDAVVTDRLSLDHINDGL
                     DMIRTGQTVRAVVVYD"
     gene            230..1099
                     /locus_tag="VN627_04715"
     CDS             230..1099
                     /locus_tag="VN627_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXD02592.1"
                     /translation="MHSEAPPDLLRWKVGDVSICVIPEVDVELPATMFFPAATIAEIA
                     DIGWLYPRFMTDNHDLKLRMQAFVVETPDRLIVVDTCYGNDKQRPGDTNMLATPFLER
                     FGQAGYDPEKVDTVLCTHLHVDHVGWNTRLIGGKWVPTFPNARYLIARVEHENWSPAD
                     REMVGDYYTDSIQPIFDAGLVDLVETDHRVSPEVSLIPTPGHTLGHVSIVIASQGEEA
                     LITGDFIHHPAQLARPEWSAFIDHDGDRARETRLAVWEKLAGSDALLCGSHFMPPVAG
                     RISKDGRGYKLIA"
     gene            1127..1813
                     /locus_tag="VN627_04720"
     CDS             1127..1813
                     /locus_tag="VN627_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD02593.1"
                     /translation="MGQAMPEVCLMVGMGESLGEALVRKFAAQGIRVAFVGRRAESVA
                     QHEQRLRAMGLAATGYAADAADEQAMADVVTRITSEWGAPTLLIYNAAVMQQGPLVDA
                     PPSAARRSFEANALGALNSVQQVLPGMRQAGRGTILISGGAAALHPHPGWGILALGKA
                     ALRSITLSLAQELAPENIRVNSVVIHGSIQYDPWYAPDRIADTFWSIYAAPDANLPVE
                     VEFKRPEEPS"
     gene            1810..2193
                     /locus_tag="VN627_04725"
     CDS             1810..2193
                     /locus_tag="VN627_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD02594.1"
                     /translation="MMTREQAVDLVTEFLARFSAMEFGRLGELMADDVIVEMPYTPGS
                     TVHHGRAECVAYLDRSVRSFVKQVKFTITDTLFDPARQAILVDHRNDGVRVDGRPYHN
                     RYLGIFDLADGRIVRWQQFLNPALL"
     gene            2283..2810
                     /locus_tag="VN627_04730"
     CDS             2283..2810
                     /locus_tag="VN627_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXD02595.1"
                     /translation="MAVFGASHLPIGVSDMDKVLPFYTRVLGFNVVSDFIQDIGPDQG
                     RDLHGGRHIRRRQVWLRQDDKAHSFAIALDEMSLGGPADHRADIYDLGIHHIGLWVDN
                     IQEIVQRAREGGHPVVMPHTSSPDGYGDDDAEGRIASVFLRDPEGNLIQCDQRIREDE
                     FTDWHSGSASKYVED"
     gene            2873..3778
                     /locus_tag="VN627_04735"
     CDS             2873..3778
                     /locus_tag="VN627_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXD02596.1"
                     /translation="MTGFTLKPLDHIGREVVGLDFAHLADREVAAALYAAWLEHGVLL
                     FREAGTSDEVHLCLSRIFGELEEYSVKEILVKDNPDLIFIGGESQKGTPKIIDGEKRA
                     GFVYYHHDTNYTPYVPKGAVLRMAITPERGGDTVWTDTRRAWEALPQHLKSRIEGLSA
                     VVSSSAGIPDRRLWGWPGHTAAYGEASPDAFEFNPGAPRVEPLVTVHPETGAKALMID
                     PFDFVKIVGMNQAEGDELFEEIAAHALRPEFSYRHHWAVHDMVAWDNRRTMHCAMGYP
                     YELSRLAYRTTLAGGMAIGAWYDGA"
     gene            3804..4610
                     /locus_tag="VN627_04740"
     CDS             3804..4610
                     /locus_tag="VN627_04740"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD02597.1"
                     /translation="MRTAGFVGIGDQGEPIAGRILAGGFPLAVWARRPDSMARLLAAG
                     ATAASTPAELGRACDYVGLCVTTDDDVAEVVETLLPAMRPGSLLAIHATVSPAGCEEL
                     ARICAQRDVRFLDAPVSGGRKVAEAGALTVMCGGDAADLAYATPVLATFSDTIIHAGP
                     AGCGQKAKIVNNSLLTANLGTAAAAIRAGEALGLDRDTLLEVLGRSSGYSRAIDICRT
                     IPAGGTMSDHGAAILTKDAMLLQESLPDDANAAALLAAARQFFVAALPRL"
     gene            4668..5273
                     /locus_tag="VN627_04745"
     CDS             4668..5273
                     /locus_tag="VN627_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXD02598.1"
                     /translation="MTVQAIIEAAERLFAKHGIDGVSMRQISQEAGSGNHFSVQYHFG
                     DKKALAEAIIANRMPDLERRRGELLAEVTRAGALTDVHALMRALLLPYCYQTDVNGEH
                     SYAAFAIHLYWHSSIPPTWQTSPEFAPMVRHIITLISQQLDLPDDIAELRLRLALVSF
                     LHVVVDRDQWRTGVHGRAVEDMWEDAVRFAVGGLTAPWLAA"
     gene            complement(5343..6656)
                     /locus_tag="VN627_04750"
     CDS             complement(5343..6656)
                     /locus_tag="VN627_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD02599.1"
                     /translation="MNDMILVSVDDHIIEPANLFERHVSAKYKDQAPRVVTFRGGEQR
                     WKFQDRLIPTIASCAVSGRKREELGAEASNYEDMRSGCYDSAARVADMNANGVLSSVC
                     FPTMTGFAGQLFLDKSDPDLMLAMIRAYNDWHVQDWCGPHPGRFIPLGFVPLWDTDLA
                     IAEIERMARMGVRTVCLPENPSALGLPSIHRDYWLPVLDALVDHDMVVSIHIGTAGPP
                     PYPSMDTPQDWFNSVINLLAAHTMADWVFSPVLRKAPDLRIALSEGCMGWVPFMLERV
                     DAAYDNHRFWTYQNLGELRPSDLIKRHFMFCFHEDQFGLEQRHKVGIDRIGWECDYPH
                     CDSTWPTSPEKLWEQVNSFPAAEIERISHANALDFFKFDPFVHVPREQATVGALRALA
                     AGHDTSERSLGGSGRHPELDPELNVLTARALQNLHEQLIKLEPAE"
     gene            complement(6694..7860)
                     /locus_tag="VN627_04755"
     CDS             complement(6694..7860)
                     /locus_tag="VN627_04755"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD02600.1"
                     /translation="MIFSANSHFIEPAHVFEGRMPRALADRAPRVIDYGEGGQAWTFA
                     EQVRPLIQCTATAGIPQEQWSGRTVVRYEDIRKGCYDPHARLADMDRDGVDVAVCYSA
                     YAGMGFGGDLFAHTDDLELAHASIRAFNDWMHEEWVAADPTRFVAVGVLSYRDPVEAA
                     REVRRNAARGFKGVLFRNPLDLGLAWTGDPVWDPLFRACEETGTILVHHTNALEGWPA
                     RAPAGSVQPPYGSVSVPFQVSALDTVNSFLWAGIGHRFPGIRILISESGGSWLPHLIR
                     RIDWALGWAPMFADGWPDPKVGPAEALARNFAFSTLEADQARSIEADHGISCWMLEDD
                     YPHMESVWPETANHFARQLAGEPADFTERFLWRNGSELFRHPMPARRAIAAQQA"
     gene            8212..9528
                     /locus_tag="VN627_04760"
     CDS             8212..9528
                     /locus_tag="VN627_04760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD02601.1"
                     /translation="MDDIPAASPAAKAARPAGNRVLLPALLAVMAVQQIDRNIINVVL
                     EPLKREFGLSDAQLGLLAGTIFASSYVIAGLPIGRLADRVNRRNLLAALLVVWSLLTS
                     LSAFCFSYAMLVLVRTGVGAAESGTQPTAMSMLSDVYPAERRATVAGLIYGSAMFGGF
                     LSFAVGGYVAANYGWRNAFLVAGLPGLLLAAFMMIAVKEPVRTAATPAAAVNVQSSFL
                     RDIRSLLFHPILGPIYLAGTICVLVVSAVSAWAISLLVRYNGLSLAEAGVIVALATSL
                     SGTCGPPLAGWLTDRAGRRSAGNVLRIPAISAAACGLGAWGFAHSFVPALSIACLCLM
                     GLTSLAHAGGSISTISRFAPPQVRALGFALFSVAGSVLGLAIGPWLVGILSDASADPQ
                     PLRSAMAMVAPVNLVAVGLFLLGARRIDRRAPDLAPDPLMAIPTKR"
     gene            9533..>10759
                     /locus_tag="VN627_04765"
     CDS             9533..>10759
                     /locus_tag="VN627_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD02602.1"
                     /translation="MTYDLVIRNGTLVDGTGSPGRQADVAVNGDRIVAIGDLAGKGRE
                     EIDATGLVVAPGFVDVHTHYDAQVFWDPRLTPSCFHGVTSVIAGNCGFSIAPLNAEAA
                     PYLTRMLAKVEGMPVAALRQGVPWNWSSFADYLARLEGRVGLNIGVMAGHSAIRRVVM
                     GERAVGSEATPAELAQMKDVLAESLAAGALGFSTTLALTHCDAEGQPVPSRHASRDEV
                     IALSGVCREFEGTSLEVIPNVGRFGEPEYRLMTDMSLAAQRAVNWNALVPSDTPGQTV
                     SQLEASDYAARHGAEVVALTVPQPMSMRINLFTGFIFEALGGFAQLFRMPVDERIEWL
                     RHPENRAELDRRGTSSESGIFQFVAKWDQLVISTSGDQSLIGRRVGDIARDRGVTPID
                     AMIDIAIADGLKTSFLV"
BASE COUNT         1834 a         3687 c         3399 g         1839 t
ORIGIN      
        1 ggaccggctc gatcatgggc ggcgccacgg ttcacgatgt cgagcggttc gtggccatgt
       61 acatggccgg cgaaatcacg ctcgacgcgg tggtgaccga tcgcctctcg ctcgatcaca
      121 tcaacgacgg cctcgacatg atccgcaccg gccagaccgt gcgggccgtc gtggtctacg
      181 actgaacgac cgatcgcgta tcgaaccacc ggcaacacag gggacgacaa tgcattccga
      241 agcgccgcca gacttgctcc gttggaaagt tggcgacgtg tcgatctgcg tcatacccga
      301 ggtcgatgtc gaactgccgg ccacgatgtt ctttccggct gccaccatcg ccgaaatcgc
      361 agacatcgga tggctctatc cccgcttcat gaccgacaac catgacctca agttgcgcat
      421 gcaggccttc gtggtggaaa cgcccgaccg gctgatcgtg gtggacacct gctacggcaa
      481 cgacaagcag cgccccggcg ataccaacat gctcgccacc cccttcctcg agcggttcgg
      541 tcaggcgggc tatgatccgg aaaaggtcga taccgtcctt tgcacccatc tccacgtcga
      601 ccacgtgggc tggaacacgc gcctcattgg cggcaagtgg gtgccgacct tccccaacgc
      661 ccgctatctg atcgcccgcg tcgaacacga aaactggtca cccgccgacc gcgaaatggt
      721 cggtgactat tacacggact cgatccagcc gatcttcgac gccggactgg tcgatctggt
      781 cgagaccgat caccgcgtct ccccggaagt cagcctgatc cccaccccgg ggcacaccct
      841 cggccatgtc agcatcgtga tcgcatccca gggggaagag gcgctcatca ccggggattt
      901 catccatcat ccggcccagc tggcccggcc ggaatggtcc gccttcatcg atcacgacgg
      961 tgaccgggcc cgcgagacac gcctggccgt ctgggagaag ctcgcaggat cggacgcgct
     1021 gctgtgcggc agccatttca tgccgcccgt ggccggccgg atcagcaagg atggacgggg
     1081 ctacaaactg atcgcctgaa ggcgggcgaa ctgaagcaat caacagatgg gacaagctat
     1141 gccggaagtg tgcctgatgg tgggaatggg cgaaagtctc ggcgaggccc ttgtccgcaa
     1201 attcgccgcg cagggcattc gcgtcgcctt cgtgggtcgg cgggcggaaa gcgtcgcgca
     1261 acacgagcag cgcctgcgcg ccatgggact cgctgcaacg ggctatgccg ccgacgccgc
     1321 cgacgaacag gccatggccg acgtggtgac caggatcacc agcgaatggg gcgcgcccac
     1381 gctcctgatc tacaacgccg ccgtaatgca acagggcccc ctggtcgacg ccccgccaag
     1441 cgcggcccgt cgttcgttcg aggcaaacgc gctgggcgca ctgaacagcg tgcagcaagt
     1501 cctgccgggc atgcgacagg ccggccgggg cacgatcctc atttccggcg gagccgccgc
     1561 ccttcatcct cacccgggct ggggcatact ggccctgggc aaggccgcgc tgcgcagcat
     1621 tacgctgtcc ctggcccagg agctcgcccc ggagaacatc cgggtgaatt ccgtcgtcat
     1681 ccacggcagc atccagtacg atccctggta tgcccccgat cggatcgcgg acacgttctg
     1741 gtcgatctat gccgcgccag atgcgaacct gccggtcgaa gtggaattca agcgcccgga
     1801 agaaccatca tgatgacgcg cgaacaggcg gtcgatctgg taaccgaatt cctcgcgcga
     1861 ttttccgcca tggaattcgg ccgcctcggc gaactgatgg ccgatgatgt gatcgtcgag
     1921 atgccctaca cgccaggcag cacggtccac cacggccgcg ccgaatgcgt ggcctatctc
     1981 gatcggtccg tccgcagttt cgtgaagcag gtgaagttca ccattaccga caccctcttc
     2041 gacccggcgc ggcaggctat cctggtggat caccgcaacg atggcgtgcg ggtcgatggc
     2101 cgcccctatc acaaccgcta cctcggcata ttcgacctcg ccgacggacg catcgtccgc
     2161 tggcagcaat tcctcaatcc cgccctgctc tgatagtatc ggatcgggcg gggcgatgat
     2221 gaaccaggca ccgcagcacc gcccccggcg cttgctgcac tcttcgaagg aaaggcttcc
     2281 ctatggcggt attcggcgct tcccacttgc cgatcggtgt cagcgacatg gacaaggtgc
     2341 tgcccttcta cacccgggtt ctcggcttca acgtcgtttc cgatttcatt caggacatcg
     2401 gcccggatca ggggcgggac cttcacggcg gccggcacat ccgccggcgc caggtctggc
     2461 tgcgccagga tgacaaggcc cattccttcg cgatcgcgct tgatgaaatg tcgctcggcg
     2521 gccccgctga tcaccgggcc gacatctacg atctcggcat tcaccacatt ggcctgtggg
     2581 tggataacat ccaggaaatc gtccagcgcg cccgcgaagg cggacatccc gtggtgatgc
     2641 cgcacaccag ctcgcccgac ggctatggcg acgacgatgc cgaagggcgg atcgccagcg
     2701 tgttcctgcg cgatccggaa ggcaacctga tccagtgcga ccagcggatc agggaggatg
     2761 aattcaccga ctggcacagc ggcagcgcgt cgaaatacgt cgaagactaa acggcttgcc
     2821 ggccacccgg tcacgggtgg aggccggccg gacaacggaa ggagatgcga aggtgaccgg
     2881 ctttaccctg aaaccgctcg accacatcgg ccgggaagtc gtcggcctcg acttcgcgca
     2941 cctcgccgat cgcgaggtcg ccgccgcgct ctatgcagcc tggctcgaac atggcgtcct
     3001 gctgtttcgc gaggccggaa cctcggatga agtccatctt tgcctctccc gcatcttcgg
     3061 cgagctggaa gaatattcgg tcaaggaaat cctggtgaag gacaatcctg acctgatctt
     3121 catcggcgga gaaagccaga aaggcacccc caagatcatc gatggcgaaa agcgcgccgg
     3181 cttcgtctac taccaccacg ataccaacta cacgccctac gtgcccaagg gcgccgtcct
     3241 gcgcatggcg atcacgccgg agcgcggcgg cgatacggtc tggaccgata cccgccgcgc
     3301 gtgggaagcc ctgccccagc atctcaaaag ccggatcgag ggcctgtcgg cagtcgtctc
     3361 gtcaagcgcc ggcatacccg accgccggct gtggggctgg cccggtcaca ccgccgccta
     3421 tggcgaggcg tcgcccgatg cgttcgaatt caatccgggc gcgccgcggg tcgagccgct
     3481 ggtcaccgtc catcccgaaa cgggcgccaa agcgttgatg atcgatccgt tcgatttcgt
     3541 gaaaatcgtc ggcatgaacc aggccgaagg cgacgaactg ttcgaagaga tcgccgccca
     3601 cgccctgcga ccggagttct cctatcgcca ccactgggcg gtgcatgaca tggtcgcctg
     3661 ggacaatcgc cgcaccatgc attgcgctat gggctacccc tacgaactgt cgcgcctggc
     3721 ctatcgcacc acgctggcgg gcggcatggc aatcggcgcc tggtacgatg gcgcctgatc
     3781 gggcggctgt ggccgcgcca ggcgtgcgga cagcgggctt cgtcgggatc ggcgatcaag
     3841 gcgaaccgat agccgggcgc atcctcgccg gcggcttccc gcttgcggtc tgggcccgca
     3901 ggccggacag catggcgcgg ctgctcgcag ccggcgcaac ggccgcgtca accccggccg
     3961 agctcggccg cgcctgcgat tatgtcggcc tgtgcgtaac gaccgacgat gacgttgccg
     4021 aagttgtcga aactctcctg ccggccatgc gcccgggcag cctcctggcc attcacgcca
     4081 ccgtttcccc ggccgggtgc gaggaacttg cccggatctg tgcccagcgc gatgttcgct
     4141 ttctcgatgc gcctgtgtcg gggggccgca aggtggccga ggccggcgcc cttactgtca
     4201 tgtgcggtgg cgacgccgcc gacctcgctt atgccacgcc cgtgctcgcc accttttcag
     4261 acacgatcat tcacgctggc ccagccggat gcgggcagaa agccaagatc gtcaacaaca
     4321 gcttgctcac cgcaaacctc ggcaccgcgg cggccgccat acgcgccggt gaggcgctgg
     4381 gactggatcg cgacacgctg ctcgaggtgc tcggtcggtc cagcggatac agccgggcga
     4441 tcgacatctg ccgcaccatc ccggccggcg ggacgatgag cgatcacggc gcggcgatcc
     4501 tgaccaagga cgcgatgctg ctccaggaaa gccttccgga tgatgcaaac gccgcagccc
     4561 tcctcgcggc cgcccgccag ttcttcgtcg ccgcgctgcc aaggctgtaa ccgcaggagc
     4621 ggcaccggtc tttatatcgg cgttccccga ggctaagggg aacggcaatg acagtccaag
     4681 ccatcatcga agccgccgag cgcctgtttg ccaagcacgg catcgacggc gtctcgatgc
     4741 ggcagatcag ccaggaagcc ggttccggca accatttctc cgttcagtac cacttcggcg
     4801 acaagaaggc gctggccgag gctatcatcg ccaatcgcat gcccgatctg gaacggcggc
     4861 gcggcgaact gctggcggaa gtgacgagag ccggcgcgct gacggatgtg catgccctga
     4921 tgcgggccct gctcctgccc tattgctacc agaccgacgt gaacggcgaa cacagctacg
     4981 ccgccttcgc catccacctc tactggcatt ccagcattcc gccgacgtgg cagacctcac
     5041 ccgaattcgc gccgatggtg cggcacatca tcactctcat cagtcagcaa ctcgatctgc
     5101 ctgacgatat cgccgaactg cggctgaggc tggccctcgt atcgtttctc cacgtcgtgg
     5161 tcgatcgcga tcagtggcgc accggtgtcc acggccgggc tgtcgaagac atgtgggaag
     5221 acgcggtgcg gtttgcggtg ggcggcctca ctgctccctg gctcgctgct tgatcgcatg
     5281 actcaaggcc gatggcaccg gccgatcgct cacccgcgat cgtccggtgc cctatccgcg
     5341 ccctattcgg cgggctccag cttgatcagt tgctcatgca aattctgcag cgcccgcgcc
     5401 gtgagcacgt tgagttcggg gtcgagctcc ggatggcgcc cgctaccgcc aagtgaacgc
     5461 tcgctggtat cgtgaccggc cgccagagcg cgcagggcgc ccacggtggc ctgctcgcgc
     5521 gggacatgga cgaatgggtc gaacttgaag aagtccagcg cattggcatg gctgatgcgc
     5581 tcgatctcgg ctgcggggaa gctattcacc tgttcccaca gcttctccgg tgaggtcggc
     5641 caggtggaat cgcagtgcgg gtagtcgcac tcccacccga tccggtcgat gcccaccttg
     5701 tgccgctgct caagcccgaa ctggtcttcg tggaagcaga acatgaagtg ccgcttgatg
     5761 aggtcggagg ggcgcagctc gcccaggttc tggtaggtcc agaagcggtg gttgtcgtaa
     5821 gcggcgtcca cccgttcgag catgaacggg acccacccca tgcagccctc cgacagggcg
     5881 atccgcaggt ccggcgcctt gcgcaggacc ggcgaaaaca cccagtcggc catggtatgg
     5941 gccgcaagca ggttgatcac cgagttgaac cagtcctgcg gggtgtccat cgacggatag
     6001 ggcggcggcc ccgcggtgcc gatgtggatc gagaccacca tgtcgtggtc aaccagggca
     6061 tccagcaccg gcagccagta gtcccggtgg atcgagggca ggccgagcgc cgagggattt
     6121 tccggcaggc aaaccgtgcg cacgcccatc ctggccatcc gctcgatctc ggcaatcgca
     6181 aggtcggtat cccacagcgg cacgaaaccg agcggaatga accggcccgg gtgcgggccg
     6241 caccagtcct gcacatgcca gtcattgtag gcgcggatca ttgccagcat caggtcgggg
     6301 tccgacttgt cgaggaagag ctggccggca aacccggtca tggtcgggaa gcagaccgag
     6361 gacagcaccc cgttggcatt catgtcggcc acgcgcgcgg cggaatcgta gcagccgctg
     6421 cgcatgtcct cgtagttcga agcttccgcg ccaagttcct cccgcttgcg gccggaaacg
     6481 gcgcaggatg cgatcgtcgg gatcagccgg tcctggaact tccaccgctg ctcaccgccg
     6541 cgaaaggtaa cgacgcgggg ggcctggtcc ttgtactttg cactcacgtg ccgttcgaac
     6601 aggttggcgg gctcgatgat gtgatcatcg acgctcacca ggatcatgtc gttcatctgc
     6661 atggtcagtc tccgcggtaa tctgtctgcc agttcaggcc tgttgggcgg cgatggcccg
     6721 gcgggccggc atcgggtgac ggaacagctc ggagccgttg cgccagagga atcgttcggt
     6781 gaaatccgcc ggttcgccag cgagttgccg ggcgaaatgg ttggcggttt caggccacac
     6841 cgattccatg tggggatagt cgtcctcaag catccagcag gaaatcccgt ggtccgcctc
     6901 gatcgacctg gcctgatccg cctcgagcgt gctgaaggcg aagttgcgtg ccagggcctc
     6961 ggccgggcca accttggggt cgggccagcc gtcggcgaac atgggcgccc agcccagcgc
     7021 ccagtcgatc cgccggatca ggtgcggcag ccaggagccg ccggattcgg aaatcaggat
     7081 gcggatgccg ggaaagcgat ggccgatccc ggcccacagg aagctgttga ccgtatccag
     7141 cgcggagacc tggaacggca ctgaaaccga accatagggc ggctgcaccg atccggccgg
     7201 agcgcgggcc ggccagccct ccagcgcatt ggtgtggtgg acaagaatcg ttccggtttc
     7261 ctcgcaggcg cggaacagcg gatcccagac cgggtccccc gtccacgcca ggccgaggtc
     7321 cagcggattg cggaacagaa cgcccttgaa gccgcgcgcc gcattgcgcc gcacctcgcg
     7381 tgccgcctcc accgggtccc ggtaggacag gacgccgacc gcgacgaacc gggtgggatc
     7441 ggcggcgacc cattcctcgt gcatccagtc gttgaaggcc cggatcgagg catgggccag
     7501 ttcgagatcg tcggtgtggg cgaacaggtc accgccaaag cccatgccgg cataggccga
     7561 atagcagacc gccacatcga ccccatcgcg gtccatgtcg gcgagccggg catgcggatc
     7621 gtagcagccc ttgcggatat cctcgtagcg cacgaccgtg cgcccgctcc actgctcctg
     7681 cggaatgccc gcagtggcgg tgcactggat cagcgggcgg acctgctcgg cgaaggtcca
     7741 ggcctgcccg ccctcgccat agtcgatcac gcggggcgcc cgatccgcca gggcgcgcgg
     7801 catgcgcccc tcgaagacat gcgccggctc gatgaaatgg ctgtttgcgg aaaagatcac
     7861 cccgcggttc tccccagctc tcgcactggc gcgatccggc caggcgggat catggtgttg
     7921 ttccgggagc atgccggggc cgcggcgttg atcgccagcc cgccacggcg cggcagcacc
     7981 ctcgaaattt cacccatgcc gtctcccgga cccactgcat gtaggccagt cctattgtcc
     8041 ttgccaagct acgacgggtg acttagaact gcaaacacaa agtgcgagca gcacggaaat
     8101 cgggagatgt gtggatgttg gccagcgcgt gcccggaatg cgtacgcccg gtcagccgtg
     8161 gtgtgccaac cgcgcatccc gccaaatccg aacggtatca tcatgctttc catggatgat
     8221 atccccgccg catcgcctgc cgccaaggcg gcccgcccgg ccggtaatcg cgtgctcttg
     8281 ccggccctcc tcgccgtcat ggccgtccag cagatcgacc gcaacatcat caatgtcgtg
     8341 ctcgaaccgc tcaagcgcga gttcgggctg agcgatgccc agctcgggct cctggccggc
     8401 acgatcttcg catcgagcta tgtcatcgcc ggcctgccga tcgggcggct ggccgatcgg
     8461 gtcaaccggc gcaacctcct cgccgcgctc ctggtcgtct ggagcctgct gacctcactg
     8521 agcgcattct gcttcagcta tgcaatgctg gtgctggtgc gcacgggcgt cggcgcggcc
     8581 gaatccggca cccagcccac ggcaatgtcg atgctgtcgg atgtgtaccc ggccgaacgg
     8641 cgggcgacag tggctggcct gatctacggc tcggccatgt tcggtggttt cctcagcttc
     8701 gcggtcggcg gctacgtcgc cgcgaactat ggctggcgca atgccttcct cgtcgccggc
     8761 ctgcccggac tgctgcttgc cgccttcatg atgatcgccg tcaaggagcc cgtgcgcacg
     8821 gccgcaacgc ctgctgcggc agtcaatgtt cagagctctt tcctccgcga catccgctcg
     8881 ctgctgtttc acccgatcct gggaccgatc tatctggccg gcacgatctg cgtcctggtc
     8941 gtctcggccg tctctgcctg ggccatttcc ctgctggtcc gctacaacgg cctgagcctg
     9001 gccgaggccg gggtcattgt tgccctggcg accagcctga gcggcacctg cgggccgccg
     9061 ctggcgggat ggctgaccga ccgcgccggc cgccgctcgg ccggaaatgt cctgcgcatc
     9121 cccgcgatct cggccgccgc ctgcggcctg ggggcctggg gcttcgccca cagcttcgtc
     9181 ccggccctgt ccatcgcctg cctgtgcctc atgggcctga cctcgctggc ccatgccggc
     9241 ggctccattt ccaccatcag ccggttcgcc ccaccccagg tccgcgcgct gggcttcgct
     9301 ctgttttccg ttgccggcag cgtgctcggc ctggcgatcg gaccgtggct ggtcgggatc
     9361 ctgagcgatg cctcggccga tccccaaccg ctgcgctcgg ccatggcgat ggtggcgccg
     9421 gtgaacctcg tggcggtggg gctgtttctg ctcggcgcgc gccgcatcga ccgccgcgcg
     9481 cccgacctcg cgcccgaccc cctcatggct attccaacga aaaggtgatc agatgaccta
     9541 cgatctcgtg atccgcaacg gcacgctcgt tgacgggacc ggctcccccg gccgccaggc
     9601 cgacgtcgcc gtcaatggcg accggatcgt cgccatcggc gatctcgccg gtaagggacg
     9661 cgaggagatt gacgccaccg gcctcgtcgt cgccccgggc tttgtcgacg tgcacaccca
     9721 ttacgacgct caggtcttct gggatccgcg gctcaccccc tcgtgctttc atggcgtcac
     9781 ctcggtcatc gccggcaact gcggcttcag catcgcgccg ctcaatgccg aagcggcgcc
     9841 ctatctcacc cggatgctgg ccaaggtcga aggcatgccc gtggccgcgc tgcggcaggg
     9901 cgtgccatgg aactggagca gctttgccga ctatctcgcc cgcctcgagg gccgcgtcgg
     9961 cctcaacatc ggggtcatgg ccgggcacag cgcgatccgc cgcgtggtga tgggcgaacg
    10021 cgcggtcggc tccgaggcca cccccgccga actggcccag atgaaggacg tgctggctga
    10081 aagtcttgcc gccggcgcct tgggcttctc gaccacgctc gccctcaccc actgcgatgc
    10141 cgaaggccag ccggtaccct cgcgccatgc ctcgcgcgac gaagtcatcg cgctgtccgg
    10201 cgtgtgccgc gagttcgaag gcacctcgct cgaggtgatc cccaatgtcg gccgctttgg
    10261 cgagccggag taccggctca tgaccgacat gtcgctggct gcccagcgcg cggtgaactg
    10321 gaacgccctc gtccccagcg atacgcccgg ccagaccgtc agccagcttg aggcgagcga
    10381 ctatgccgct cgtcacggcg ccgaagtcgt cgcgctgacc gtgccccagc cgatgtcgat
    10441 gcggatcaac ctgttcaccg gcttcatctt cgaagcgctc ggcggttttg cccagttgtt
    10501 ccgaatgccg gtggacgagc gcatcgaatg gctgcgccat cccgaaaacc gtgcggaact
    10561 tgatcgtcgc ggcacgtcct cggaatccgg catcttccag ttcgtcgcca agtgggacca
    10621 gctggtgatc tccacctccg gtgaccagtc gctgatcggc cggcgggtcg gcgatatcgc
    10681 gcgcgatcgc ggcgtcacac cgatcgacgc catgatcgac atcgccatcg ccgatggcct
    10741 caagacctcg ttcctggtc
//
LOCUS       DATNBV010000196         2307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1696048,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000196 DATNBV010000000
VERSION     DATNBV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696048"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VN627_04770"
     CDS             complement(<1..1091)
                     /locus_tag="VN627_04770"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HXD02603.1"
                     /translation="MGVEVVSLGCRMNLSESERLRTLLAGADDLVVVNSCAVTGEAVR
                     QTRQAIRRARRDRPTARLLVTGCAAEVERAALGAMPEVNGLVANATKLDPRAWNAPAS
                     AVVAPRSHTRAYVAVQNGCDHACTFCVIPQGRGPSRSLPVSAVLREVERHLDEGSREV
                     VLTGVDLTSWGHDLPDAPRLGELVVAILAAFPALPRLRLSSVDGVEIDPLLLELIAGE
                     ARVMPHVHLSLQHGHDLVLKRMKRRHCRADAVALVEHLRAARPNLAIGADLIAGFPTE
                     DDAMHAANRSVVRELGVVHGHVFPYSARGGTPAARMPQVDAATIRRRAAELRADVAAE
                     RADWLQSLTGQPLAVLAERDGTGHAPNFAR"
     gene            complement(1094..1921)
                     /gene="dapF"
                     /locus_tag="VN627_04775"
     CDS             complement(1094..1921)
                     /gene="dapF"
                     /locus_tag="VN627_04775"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXD02604.1"
                     /translation="MAGSSTRVPFIKMHGLGNDFVVLDARTGGVPPVDTRLAAALADR
                     HRGIGCDQLILIEPSATADLRMRIFNADGSEVEACGNATRAVGLLHGKPARIETLAGV
                     LEARPDGAGIAVDMGRPRFGWDEIPLAWAMDTLHLPVGWEDLADPVAVNVGNPHVIFF
                     VADCAAVDLARLGPLIETDPLFPERVNVNVATIESRTAIRLRVWERGAGLTLACGTGA
                     CATAIGAMRRGLVERTVTVTLPGGALTITWTDEDRILMSGPATEAFRGSFDPAAYGL"
     gene            complement(2057..>2307)
                     /locus_tag="VN627_04780"
     CDS             complement(2057..>2307)
                     /locus_tag="VN627_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXD02605.1"
                     /translation="TIVQAITSLGTGLGLPITAEGVENAEVVAELQRLGQFMAQGYLY
                     GRPEPAEKTRERLAGLGMLNTADGLAPVPADDKLSDVA"
BASE COUNT          368 a          803 c          809 g          327 t
ORIGIN      
        1 cgggcgaagt ttggcgcatg gccggtcccg tcgcgttcgg caagcactgc gaggggctgg
       61 cctgtcaggg attgcaacca atccgcgcgc tcggccgcaa catcggccct gagttcggcg
      121 gcgcgccgcc ggatcgtcgc cgcatcgacc tgcggcatgc gcgcggccgg cgtgccgccg
      181 cgggcggaat aggggaagac atggccgtga accacgccga gctcgcgcac caccgagcga
      241 ttggcagcgt gcatggcatc atcctcagtg gggaatccgg cgatgaggtc cgcgccgatc
      301 gcgaggttag gccgcgcggc ccgcaggtgc tcgaccagcg ccacggcatc ggcccggcag
      361 tgccgccgtt tcatccgctt gagcacaagg tcatgcccgt gttgcagcga gaggtgcaca
      421 tgcggcatga cccgagcctc gcccgcgatc agttccagca gcagcggatc gatctccacc
      481 ccgtcgaccg aggaaagccg cagccggggc agggcaggga aagccgcgag gatcgcaact
      541 accagttcgc ccaggcgggg cgcatctggc aggtcatgcc cccaggaggt taggtcaacg
      601 ccggtgagca caacctcgcg cgagccctcg tcgaggtggc gctcaacctc gcgcagcacg
      661 gcgctgaccg gcagcgagcg gctgggcccg cgtccttgcg gaatgacgca gaaggtgcag
      721 gcatggtcgc acccgttctg cacggcgaca taggcgcggg tatggctgcg cggagcgaca
      781 acggcactgg ccggggcgtt ccacgcccgc ggatcgagct tggtcgcgtt cgccaccagc
      841 ccattgacct cgggcatggc ccccagcgcc gctcgctcca cctcggccgc gcagccggtt
      901 acgagcaggc gcgccgtcgg ccggtcccgg cgcgcgcggc ggatggcctg ccgggtctgg
      961 cgcacggcct cgccggtgac ggcgcaggaa ttgaccacga caaggtcgtc cgccccggcc
     1021 agcaaggtcc gcagccgctc gctttccgac aggttcatcc ggcagccgag ggaaacgact
     1081 tcgacgccca cgctcagagc ccgtaagcgg ccggatcgaa gctgccgcgg aaggcctcgg
     1141 tcgccgggcc gctcatcagg atgcggtctt cgtccgtcca ggtgatcgtc agcgctccgc
     1201 cggggagggt gaccgtgact gtgcgctcca ccaatccgcg ccgcatcgcc ccgattgccg
     1261 tggcacaggc gcccgtgccg caggccagcg tcagcccggc gccgcgttcc cataccctga
     1321 ggcggatcgc cgtgcggctt tcgatcgtgg cgacattgac gttgacgcgc tcgggaaaca
     1381 gcgggtcggt ctcgatcagc gggccgagcc gggcgaggtc aaccgcggcg caatcggcca
     1441 cgaagaagat gacatggggg ttgcccacgt tgaccgcaac cggatcggca aggtcttccc
     1501 agccgaccgg gaggtgcagc gtgtccatcg cccaggcgag cgggatctcg tcccagccaa
     1561 accttggccg gcccatgtcc accgcgatcc ccgcgccgtc aggccgcgct tccagcacgc
     1621 cggccagggt ctcgatccgg gccggcttgc catggagcag tccgacggcg cgcgtggcat
     1681 tgccgcaggc ttccacctcg ctgccgtcgg cgttgaagat ccgcatgcgc aggtcggccg
     1741 tggcggaggg ttcgatcagg atcagctgat cgcagccgat ccctcggtga cgatcggcca
     1801 aggctgccgc cagccgcgtg tcgacgggag gcacgccccc ggtccgcgcg tcgagcacga
     1861 cgaaatcatt gccgaggccg tgcatcttga tgaacggcac gcgagtagag gagccagcca
     1921 tggcggcgat ctaggcgctg cgccgcagcg cggcaagtgc ggcgtcaggc gggctgctcc
     1981 ccgctcacac actcagcatg acgagtagga caggcggaga ctggtggaac gaagattgct
     2041 gcgggcggca agcgcttcac gccacgtccg agagcttgtc gtcagccgga accggcgcca
     2101 ggccatcggc cgtgttgagc atgcccagtc cggccagccg ctcgcgcgtc ttttccgcgg
     2161 gctcaggccg accatagagg tagccctggg ccatgaactg gccgagccgc tgcaattcgg
     2221 ccaccacttc ggcgttttcg acgccttcgg ccgtgatcgg caggccaagg cccgtgccga
     2281 gcgaagtgat cgcctggacg atggtcg
//
LOCUS       DATNBV010000197         2282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1696819,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000197 DATNBV010000000
VERSION     DATNBV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696819"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="VN627_04785"
     CDS             <1..156
                     /locus_tag="VN627_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXD02606.1"
                     /translation="LGYRIFLVRRYLSMDIILPYVAWISLLAILTDWALLTISRKAFP
                     WAHGDAR"
     gene            153..944
                     /locus_tag="VN627_04790"
     CDS             153..944
                     /locus_tag="VN627_04790"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXD02607.1"
                     /translation="MSPDTAPILEFRDVWVEYGDKVVLERVNLAIRPGEFVSVVGPSG
                     AGKSTFLRLILGQEGPSRGEVLLDGTPLAPECSPDRGVVFQRYSVFPHLTALGNVLFG
                     LECAHAPLTARLFGTARARARGEAVAMLEAVGLGHSLDVYPAQMSGGMQQRLAVAQAL
                     VKHPRVLLLDEPFGALDPGIRLDMHDLVGKLWQEHRLTVVMVTHDINEAFKLGTRVLA
                     FDKRRHDPHAPNRYGATAVYDVTLDRKTRAAGPPAELLAAADAAA"
     gene            complement(955..1731)
                     /locus_tag="VN627_04795"
     CDS             complement(955..1731)
                     /locus_tag="VN627_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXD02608.1"
                     /translation="MTMVTSRAADCSDAEWAVRVELAACYRAVDLFGMTDLVHNHITA
                     RVPGPDHHILINAYGLHYEEICASNLLKIDLDGNIVTRPEGLPYGVNLAGYVIHSAVH
                     GARPDVACVLHTHTRAGVAVSAMDEGLLPISQTALRFHGRLGYHDFEGPATDLAERER
                     LIAHLGQDYALILRHHGLLTCGRTIGEAFTLMQRLDTSCKIQVDAMAGTVTLPTEQSQ
                     ARTAAMFAPTANDPGAPPLGTIEWAAILRQLDRKDPSYRN"
     gene            complement(1737..2060)
                     /locus_tag="VN627_04800"
     CDS             complement(1737..2060)
                     /locus_tag="VN627_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02609.1"
                     /translation="MKKYKLLVLSNCAPGTDDEFNRWYNEVHIPEVLQVPGFTGAWRG
                     PISEVTAEGSEHRFCAVYDFESDDVGATLGELAGRAADGRMHMSDTLDLAGVSMRAFE
                     VQSQQ"
BASE COUNT          348 a          778 c          771 g          385 t
ORIGIN      
        1 cttggctacc gcatcttcct cgttcgccgc tacctctcga tggacatcat cctaccctat
       61 gtcgcctgga tcagcctgct ggcgatcctg acagactggg cgctgctcac catcagccgc
      121 aaggccttcc cctgggccca cggagacgcg cgatgagccc ggataccgct ccgatcctcg
      181 aattccgcga cgtttgggtc gaatatggcg acaaggtcgt gctcgaacgg gtcaacctgg
      241 cgatccggcc gggcgagttc gtctcggtgg tcggcccctc gggcgcgggc aagagcactt
      301 tcctgcgcct gatcctcggc caggagggcc ccagccgggg cgaggtcctg ctcgacggga
      361 cgccgctcgc gcccgaatgt tcgcccgatc gcggcgtggt gttccagcgc tattcggtgt
      421 tcccgcacct gaccgcgctt ggcaacgtgc tgttcgggct tgaatgcgcg catgcgccgc
      481 tgacagcgcg gttgttcggc acggcccgcg cccgggcgcg cggcgaggcc gtggccatgc
      541 tcgaggcggt tggtctcggc cacagcctgg atgtctaccc ggcgcagatg tcgggcggga
      601 tgcagcagcg gctggccgtg gcccaggccc tggtcaaaca cccgcgcgtg ctcctgctcg
      661 acgaaccctt cggcgcgctc gatcccggca tccggctcga catgcacgac ctggtcggca
      721 agctgtggca ggaacaccgg ctgaccgtgg tcatggtcac ccacgacatc aacgaggcgt
      781 tcaagctcgg cacccgcgtg ctggctttcg acaagcgccg ccacgacccc catgcgccca
      841 accgctacgg cgcgacggcg gtctatgacg taacgctcga tcgcaagacc agggcggccg
      901 ggccgccggc ggaactcctc gccgccgccg acgctgcggc ctgagcggcc cgcctcagtt
      961 gcggtaggag gggtccttgc ggtccagctg gcgcaggatc gcggcccatt cgatcgtacc
     1021 gagcggcggc gcacccggat cgttggcggt gggcgcgaac atcgcggcgg tgcgagcctg
     1081 cgattgctcg gtcggcaggg tgaccgttcc cgccatggca tcgacctgga tcttgcagga
     1141 ggtatccagc cgctgcatca gggtgaaggc ctcgccgatg gtccgcccgc aggtcagcag
     1201 gccgtggtgt cgcaagatca gcgcatagtc ctgccccagg tgggcgatca ggcgctcgcg
     1261 ctcggccagg tcggtcgccg ggccctcgaa atcatgatag ccaagccggc catggaagcg
     1321 cagcgccgtc tgcgagatcg gcagcagccc ttcgtccatg gccgagacgg caacgcccgc
     1381 ccgggtgtgg gtgtgcagca cgcaggcgac atccgggcgc gccccgtgga ccgccgaatg
     1441 gatcacgtag ccagccaggt tgacaccata gggcaagccc tcgggccggg tgacgatgtt
     1501 gccgtccaga tcgatcttga gcaggttcga ggcgcagatt tcctcgtagt gcaggccata
     1561 ggcgttgatc aggatatgat gatccggccc cggcacccgc gcggtgatgt ggttgtggac
     1621 gaggtcggtc atcccgaaca ggtcgaccgc gcggtagcag gccgcgagtt cgacccggac
     1681 cgcccattcg gcatcgctgc aatcggcggc ccggctcgtg accatggtca tcggctctat
     1741 tgctgcgact ggacttcgaa ggcgcgcatc gagacgccgg ccaggtcgag cgtgtcggac
     1801 atgtgcatcc ggccatcggc ggcgcggcca gcaagctcgc ccagggtggc gccgacgtcg
     1861 tcgctctcga aatcgtagac agcgcagaag cggtgctcgg aaccctcggc cgtgacctcg
     1921 gagatcgggc cgcgccaggc gccggtgaag ccggggacct ggagcacttc gggaatgtgc
     1981 acctcgttgt accagcggtt gaattcgtca tccgtgcccg gggcgcagtt gctgagcacc
     2041 agcagcttgt acttcttcac gcggcctctc ctcttttatt ccgctttccg gaacggagat
     2101 tgggaccggc gcggcgccgt ggtcaaccgc ggccggtgag gacccgcgat tcagccccta
     2161 tgcgatagag gccattttac cgaggcgaca gacggcccgt ttccggctag ctgccgcgca
     2221 tgagctgcca ctgttcactc ccgcgccagc catgctgacc cgcctgcgcg gagcgctcga
     2281 cc
//
LOCUS       DATNBV010000198         8357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2670038,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000198 DATNBV010000000
VERSION     DATNBV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8357
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2670038"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..766
                     /locus_tag="VN627_04805"
     CDS             2..766
                     /locus_tag="VN627_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HXD02610.1"
                     /translation="MANFTRNRAFFVNAAVALAFAAIGALGGWLWESHRIGADPAARL
                     AGEDRAAIEQVIHDYLMAHPEVLPKAMAELERRQNAHQLASVRGDVERAVPGAVLGNP
                     QGKVVLVEFTDYACAYCRKSLGDVDALIAANPDLKVVVRELPILTAESADAAKMALAA
                     AEQGKYAEFHKAMFASARPNAETIAAAAQAAGLDMARARAVIARPETEAELVRNLDLA
                     KTLGFTGTPSWVIGDALLTGAVGLDQLSKAVTEARS"
     gene            868..2028
                     /locus_tag="VN627_04810"
     CDS             868..2028
                     /locus_tag="VN627_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXD02611.1"
                     /translation="MAFLPFHPTPFFANKNQAFWRLQAVGWGGALLLRAMSSIANGQP
                     ASFLVLVLIATVTGFSISLILSVIYRLLISQRALVTWGVTALVLPVAVTLYAFIDAWV
                     IGLYRPESGASFAQLLIGVFYLDMTLLAAWSALYYAINFYLQIEEQNDQLIRLENQAT
                     QAQLAMLRYQLNPHFLFNTLNSISTLVLLKQTEPANAMLSRLSAFLRYTLVNEPAGRV
                     TVAQEVETLKLYLDIERMRFEERLRTTFNIDDSTRAGLLPSLLLQPLVENAIKYAVGT
                     QESGAEITITTQLVGSLLRITVSDTGPGLQNTAADNRLSGVTFDGGEPVSTGVGLANI
                     RDRLAQAYGDRHRFEIIDPPEGGFTVLIEIPFERREPAPASAAAARPALAAG"
     gene            2079..2888
                     /locus_tag="VN627_04815"
     CDS             2079..2888
                     /locus_tag="VN627_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HXD02612.1"
                     /translation="MTIRTILVDDEKLAIQGLQLRLEKFPDVEVIDTCSNGREAIRKI
                     KTEKPDLVFLDIQMPGFDGFSVVKGVMEIEPPLFVFVTAYQEHAVRAFEANAVNYLMK
                     PVDEDKLADTLDRVRTRLAEKRSAEEAEKLKNVLAEVAPDAIDQMPEDEGDAAALGAG
                     RYEKLINIKDRGQIFRVDVDSIEHIEAAGDYMCIRTADNSLILRETMKDLERRLDPRV
                     FQRVHRSTIVNLNQVRQVKPHTNGECFLVLGSGAQVKVSRSYRDVVARFVH"
     gene            2933..3787
                     /gene="nadC"
                     /locus_tag="VN627_04820"
     CDS             2933..3787
                     /gene="nadC"
                     /locus_tag="VN627_04820"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HXD02613.1"
                     /translation="MTFAIPGFDLAAFVAATLAEDLGEGWPGGGRDVTSESVIPANAR
                     FAGVMDSRDAIVVCGLPIAAAFFRALDPAVEIEILHEEGARVPAGTELMRLSGKARAM
                     LTAERSALNTVQHLSGVATMTRQYVDAMQGHATLLDTRKTIPGLRHLEKYATRTGGAK
                     NHRLGLWDAAMIKDNHIAVAGSIGQAVGAARAAGVEVICEVDHLGQIEPALAAGAGHL
                     LLDNMDPAMLREAVAIVNGRVPTEASGGVRLDTIAAIAATGVTYVSVGRLTQSAPAAD
                     IGLDFTPL"
     gene            3784..4479
                     /locus_tag="VN627_04825"
     CDS             3784..4479
                     /locus_tag="VN627_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HXD02614.1"
                     /translation="MKALAAGLLALLPTPALAQAYQCSLPPRLAPLPPVRPDGPVRRT
                     PIAGYSLAVSWSPEFCRQDRDPASVQCSGRNGRFGFVLHGLWPEAPAGPPPQWCALTP
                     RPQPETVRQALCATPVPWLIEHEWAKHGSCMAQSPEAYRGIARHIWARFKWPDADRLS
                     LRPGLTVGDLREGVALANPGLPRASIGVVLSEQGWLRELRICHNRQYRPAACPRAAWG
                     PGDGKPLKIWRGL"
     gene            complement(4488..4943)
                     /locus_tag="VN627_04830"
     CDS             complement(4488..4943)
                     /locus_tag="VN627_04830"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HXD02615.1"
                     /translation="MTRWPLPEPMRLALEQARKGAGEGEVPIGAVIVKDGEVIVMAHN
                     QPRTLHDPTAHAELLAIRRAAQRLGNERLDGCELWVTLEPCAMCAGAIAHARIARVYY
                     AAPDPKGGAVEHGSRVFEQAQCLHRPEVYSGMGEAEASGLLREFFAAKR"
     gene            5175..5465
                     /gene="rpmB"
                     /locus_tag="VN627_04835"
     CDS             5175..5465
                     /gene="rpmB"
                     /locus_tag="VN627_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXD02616.1"
                     /translation="MSRICELTGKGRQVGHNVSHANNKTKRVFLPNLQNVTLLSEGLD
                     RSFKFRVSTHGLRSVEHNGGLDNWLLKTADAKLSPRAVKVKRELAKKAAAAA"
     gene            5632..5808
                     /locus_tag="VN627_04840"
     CDS             5632..5808
                     /locus_tag="VN627_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HXD02617.1"
                     /translation="MTSTLQALVRDEAGATAIEYGLIAALIAVAIMTTVSGLGSQLNS
                     TFSMTSSQLNAANS"
     gene            5936..6109
                     /locus_tag="VN627_04845"
     CDS             5936..6109
                     /locus_tag="VN627_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HXD02618.1"
                     /translation="MTTLNRLVRNEDGATAIEYGLIAALIAVAVMTTVAGLGSQLDTT
                     FSKTSSQLTAANS"
     gene            complement(6145..7431)
                     /locus_tag="VN627_04850"
     CDS             complement(6145..7431)
                     /locus_tag="VN627_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HXD02619.1"
                     /translation="MLKRIATGDVELGMFIHKLEGNWFSHPFWKSRFLLQDEHLLSQL
                     QRSAVPGVVIDTAKGLDTAAIPAAPQHKAAPAAPMALQRGARRAAAARLGASRTAERD
                     AALRSVAPRTMVQEFGLATRVAEKGRRTISRAFLEARLGKAIQPQAVEPVVDDIFASI
                     QRNAQAFNGLMRCKRETEFVYRHALAVCALMISLGRAMKLPADEIRLAGMAGLLLDVG
                     VGSLGLDLGSAGDLRDIAPDLLRGHVVTGSELLVAGGVPDPVVQACLQHHERLDGSGY
                     PYGLPAEHIGRYGRMAAIYDAYDLLVDDTGGHAALDPAAAIEELARAGAGFDPALVGL
                     FTEALGMHPIGSVVDLESGRVAMVVAQDESDPTRPRVRVFWSRNEQRALPPSDIALAH
                     CFGEDRIVGRADLADLIPGEAAPLRERLFAGACTAG"
     gene            7671..8318
                     /locus_tag="VN627_04855"
     CDS             7671..8318
                     /locus_tag="VN627_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXD02620.1"
                     /translation="MDDPVRPGTGTDPETDLRAAPRVSLLIRPAKLIAPSGEYLCVLR
                     DASATGVKVRCFHPLPAQSMVLELGNGDRYDVERMWEQGDHAGLRFRQAIPVEYLIRE
                     TGPYPKRPVRLRLTLSALLVAGRDVLRAAVLDLSQQGARIECGEQLARDRQFRLEIPG
                     LAPIVAKVRWRRPPAAGLVFEETFRLEDFARLAFRLQPLPEPPGEDDDNPFKRLA"
BASE COUNT         1357 a         2993 c         2628 g         1379 t
ORIGIN      
        1 catggccaat ttcacccgca accgcgcgtt cttcgtcaat gccgccgtcg ccctcgcctt
       61 cgccgcgatt ggcgcccttg gcgggtggct gtgggaatcc caccgcatcg gtgccgatcc
      121 cgcggcgcgc ctcgccgggg aggatcgcgc cgctatcgag caggtgatcc acgattacct
      181 gatggcccac cccgaagtgc tgcccaaggc catggccgag ctggagcgcc gccagaacgc
      241 gcaccagctc gccagcgtgc gcggcgatgt ggagcgggcc gtgcccggcg cggtgctcgg
      301 caatccgcag ggcaaggtcg tccttgtcga attcaccgac tatgcttgcg cctactgccg
      361 caagagcctg ggcgacgtcg atgcgctgat cgcggccaat cccgatctca aggtggtggt
      421 ccgcgagctg ccgatcctca ccgcggaaag cgccgatgcg gccaagatgg ccctcgccgc
      481 ggccgagcag ggcaaatatg ccgagttcca caaggccatg ttcgccagcg cccggccaaa
      541 tgccgaaacc attgccgctg ccgcccaggc cgccggcctc gacatggccc gggcccgcgc
      601 cgtcatcgcc cggcccgaaa ccgaggccga actggtccgc aacctcgatc tcgccaagac
      661 gctgggcttc accggcacgc ccagctgggt catcggcgat gccctgctga ccggcgcggt
      721 cgggctggac cagctgtcca aggcggtgac cgaagcgaga tcttgatggg gtgtgccctc
      781 cacatccgtg gagggcttgc gggccggtgc cgcaaggaaa ccgggatggg ctttccgcat
      841 tcagcaaagt tgcgctagaa cccgcccatg gccttcctgc cgttccaccc caccccgttc
      901 ttcgccaaca agaaccaggc cttctggcgc ctccaggcgg tcggctgggg cggggcgctg
      961 ctgctgcgcg ccatgtcgtc gatcgccaat ggccagcccg cctcgttcct cgtcctcgtg
     1021 ctgatcgcca cggtcaccgg cttttcgatc tccctgatcc tctcggtcat ctaccgcctg
     1081 ctgatcagcc agcgcgccct cgtcacctgg ggggtcaccg ccctcgtcct gccggtggcg
     1141 gtcacgctct acgccttcat cgatgcctgg gtaatcgggc tttaccggcc ggagagcggc
     1201 gccagcttcg cgcagctgct gatcggcgtg ttctacctcg acatgaccct gctcgcggca
     1261 tggtcggcgc tctactacgc gatcaacttc tacctccaga tcgaggagca gaacgatcag
     1321 ctgatccggc tggaaaacca ggcgacccag gcccagctgg ccatgctgcg ctaccagctc
     1381 aacccccatt tcctgttcaa cacgctgaac tcgatctcca ccctcgtcct gctcaagcag
     1441 accgagccgg ccaatgccat gctcagccgg ctctccgcct tcctgcgcta tacgctggtg
     1501 aacgagcccg ccgggcgggt caccgtcgcg caggaagtgg aaaccctgaa gctctacctc
     1561 gatatcgagc gcatgcgctt cgaggaacgg ctgcgcacca ccttcaacat cgacgattcc
     1621 acccgcgccg gcctgctgcc ctcgctgctg cttcagccgc tggtcgagaa tgcgatcaaa
     1681 tatgccgtgg gcacgcagga gagcggggcc gaaatcacca tcaccacgca actcgtcggc
     1741 tcgctgcttc gcatcaccgt ctccgacacc gggccgggat tgcaaaacac cgccgccgac
     1801 aacaggctgt ctggcgtgac tttcgatggc ggcgaacccg tgtccaccgg cgtgggcctt
     1861 gcgaacatcc gcgaccggct ggcccaggcc tatggcgacc ggcaccgctt cgagatcatc
     1921 gatccgccgg aaggcgggtt caccgtcctg atcgaaatcc cgttcgaacg ccgcgaaccc
     1981 gcgcctgcga gcgcggcggc tgcccgcccc gcgctcgcgg cgggttgaaa ccaatcgccg
     2041 cgccgcccgt atgggaggca cgcaatacaa ggtgaggcat gaccattcgc acaatcctcg
     2101 tcgatgacga gaagctagcg atccaggggc tccagctcag gctcgagaag ttccccgacg
     2161 tggaggtgat cgacacctgc tccaacgggc gcgaggcgat ccgcaagatc aagaccgaaa
     2221 agccggacct cgtcttcctc gacatccaga tgccggggtt tgacggtttc tccgtggtca
     2281 agggcgtgat ggagatcgaa ccgccgctgt tcgtcttcgt caccgcctat caggagcacg
     2341 ccgtccgcgc cttcgaggcc aatgccgtga attacctgat gaagccggtc gacgaggaca
     2401 aactcgccga cacgctcgac cgcgtccgca cgcgcctcgc cgaaaagcgc agcgcggagg
     2461 aggcggaaaa gctcaagaac gtccttgccg aagtggcgcc cgatgccatc gaccagatgc
     2521 ccgaggacga aggcgatgcc gccgccctcg gcgccgggcg ttacgaaaag ctcatcaaca
     2581 tcaaggatcg cgggcagatc ttccgcgtcg atgtcgacag catcgagcat atcgaggccg
     2641 cgggcgatta catgtgcatc cgcactgccg acaacagcct gatccttcgc gaaacgatga
     2701 aggacctcga acgccgtctc gatccgcgcg tgttccagcg cgtccaccgc tcgactatcg
     2761 tcaacctcaa ccaggtccgc caggtcaagc cccacaccaa cggcgaatgc ttcctcgtgc
     2821 tcggttcggg ggcccaggtg aaggtttcgc gctcctaccg cgacgtggtg gcgcgcttcg
     2881 ttcactgatc cggggttgcg ctgacggcgg tggtcggcga aatgccgagc ccatgacctt
     2941 cgccatcccc ggtttcgacc tcgccgcctt cgtcgccgcc actctcgccg aggatcttgg
     3001 cgaaggctgg cccggcggcg gccgcgatgt caccagcgag agcgtgatcc ctgcgaacgc
     3061 ccgctttgcc ggggtgatgg acagccgcga tgcgatcgtc gtctgcggcc tgccgatcgc
     3121 cgcggccttc ttccgcgcgc tcgatccggc ggtcgagatc gagatcctgc atgaggaagg
     3181 cgcccgcgtc cccgccggga ccgagctgat gcgcctgtcc ggcaaggccc gggccatgct
     3241 caccgcggaa cgcagcgccc tcaacacggt ccagcatctc tccggcgtgg cgacgatgac
     3301 ccgccagtat gtcgatgcca tgcagggtca cgccaccctg ctcgataccc gcaagaccat
     3361 ccccggcctt cgccacctcg aaaaatacgc cacccgcacc gggggcgcga agaaccaccg
     3421 gctcggcctg tgggatgccg ccatgatcaa ggacaaccat atcgcggtcg ccggatcgat
     3481 cggccaggcc gtgggcgcag cgcgcgccgc cggggtcgag gtgatctgcg aggtcgatca
     3541 tctcggccag atcgaaccgg ccctggcggc cggggcgggt cacctgctgc tcgacaacat
     3601 ggaccccgcc atgctgcgcg aggccgtcgc catcgtgaac ggccgggtgc cgaccgaggc
     3661 ttcgggcggc gtccgcctcg atacgatcgc cgcgatcgcc gctacgggag ttacctacgt
     3721 ctcggttggc cgcctcaccc agtcggctcc ggctgccgat atcgggctgg acttcacccc
     3781 gctgtgaagg ccctcgccgc cggcctcctg gcgctgctgc ccacgccggc actggcccag
     3841 gcctaccagt gcagcctgcc accccgactg gcgccgctgc ccccggtcag gcccgacgga
     3901 ccggtgcggc gcacgcccat cgccggttat agcctcgcgg tctcgtggtc gcccgagttc
     3961 tgccggcagg atcgcgatcc cgcctcggtc cagtgttccg ggcgcaacgg ccggttcggc
     4021 ttcgtgctcc acggcctctg gcccgaagcg cctgccggac cgccgccgca atggtgcgcg
     4081 ttaaccccgc gcccgcagcc cgaaacggtg cgccaggctc tctgcgccac gccggtgccg
     4141 tggctgatcg agcacgaatg ggccaagcac ggctcctgca tggcccagtc ccccgaagcc
     4201 tacaggggca ttgcccgcca catctgggca aggttcaaat ggcccgatgc cgaccgcctg
     4261 tcgctgcgcc ccggtctcac cgtgggtgac ctgcgcgaag gcgttgcgct ggccaatccc
     4321 ggcctcccgc gcgcatcgat cggagtggtg ctgagcgagc agggctggct gcgcgaactg
     4381 cgcatctgcc acaatcgcca gtatcggccc gccgcctgcc cgcgtgccgc ctggggtccg
     4441 ggcgatggaa aaccgctgaa gatctggcgc gggctctaga tcacgctcta gcgtttcgcc
     4501 gcgaagaatt cccgcagcag gcccgatgcc tcggcctcgc ccatgccgga atagacttcg
     4561 ggccggtgca ggcactgtgc ctgctcgaac acccgcgacc cgtgctccac cgcgccgccc
     4621 ttgggatcgg gcgcggcata atagacccgc gcgatccgcg catgggcaat ggcccccgca
     4681 cacatcgcgc agggctccag cgtcacccac agctcgcagc catcgagccg ctcgttgccg
     4741 agcctttgcg cggcccgccg aatcgccagc aactcggcat gggctgtcgg atcgtggagc
     4801 gtgcgaggct ggttgtgcgc catgacgatc acttcgccgt ccttgacgat taccgccccg
     4861 atcggcactt cgccctcgcc cgcccccttg cgcgcctgct ccagcgccag gcgcatcggt
     4921 tcgggaaggg gccaccgggt catggtgctg cgctaggacc ggcacgggtg cgcggcaagc
     4981 gtgtctttgt cgggtacgcc ctccacatcc gtggagggct tgcgggaccg gcccgctccc
     5041 cgcaaggcag atgccagcct gccggtccct gcacgatgac tccaacttga ccttgggggg
     5101 gtgaatcggt aagggcgcgc ttcttcaagt tttcggcccc tgttcgggca ccagattctg
     5161 tagagagcaa agccatgtcc cgtatttgcg aactgaccgg caagggccgc caggtcggcc
     5221 acaacgtcag ccacgccaac aacaagacca agcgcgtctt cctgcccaac ctgcagaacg
     5281 tcacgctgct ctcggaaggg ctggaccgca gcttcaagtt ccgcgtgtcg acccacggcc
     5341 tgcgctcggt ggagcacaat ggcgggctgg acaactggct gctgaagacg gctgatgcca
     5401 agctttcgcc ccgcgcggtg aaggtgaagc gtgagctggc caagaaggcc gccgccgccg
     5461 cctgattcgc ccctgcggcg aacaacgatg gacgcgccgg gcaaccggcg cgttttttcg
     5521 ttttggcgca aggcattgcg ccgattgttc cggccaggcg tttacgtctc tttcactatc
     5581 cttcggcata gatggcgacg gaccctgggg gctaccagga gtgagttcgc catgaccagc
     5641 accctgcagg ccctcgtccg cgacgaggcc ggcgccaccg ctatcgaata cggcctgatc
     5701 gccgccctca tcgccgtcgc catcatgacc acggtttcgg gactgggctc ccagctcaat
     5761 tccaccttct cgatgacgtc gtcgcagctc aacgccgcca actcgtagca gcgcccccgg
     5821 aaatgggcgg tttcaacccg gcgaaaccgc ccgtttccag cctttaccga tcatttacta
     5881 tcttaaggga taatcttctc ggttctaggg gagaacccgg aggatatttg tcatgatcac
     5941 cacgctcaac cgccttgtcc gcaatgaaga cggcgccacc gccatcgaat atggcctgat
     6001 tgccgcgctc atcgccgtcg ccgtgatgac cacggttgcc ggcctcggct cgcagctcga
     6061 caccactttc tcgaagacct cgtcgcagct caccgccgcg aactcgtaag gtctggcggt
     6121 ccggcggcgg ccctgcccgc agggtcagcc cgcggtacag gctccggcga acagccgctc
     6181 gcgcagcggc gcggcttcgc cggggatcag gtccgccagg tcggcccggc cgacgatgcg
     6241 gtcctcaccg aaacaatggg caagggcgat gtcgctgggc ggcagcgccc tttgctcgtt
     6301 acgcgaccag aacacccgca cccgcggccg cgtggggtcg ctctcgtcct gggcgacgac
     6361 catcgccacc cgtcccgatt cgaggtcgac caccgaaccg atcggatgca tgcccagcgc
     6421 ctcggtgaac aggccgacga gggcgggatc gaaccctgct ccggcccgcg cgagctcctc
     6481 gattgctgcc gccgggtcga gcgcggcatg tcccccggtg tcatcgacca gcaggtcata
     6541 ggcatcgtag attgcggcca tgcggccgta gcggccgatg tgctcggcgg ggaggccata
     6601 gggatagccg ctgccgtcca gtcgctcatg gtgctgcagg caggcctgga ccaccgggtc
     6661 cggcactccc cctgccacca gcaactcgct gccggtcacc acgtggccgc gcagcaggtc
     6721 aggggcgata tcgcgaaggt ccccggcgga ccccagatcg aggccgagac tgcccacgcc
     6781 cacgtcgagc agcagcccgg ccattccggc aaggcggatc tcgtcggccg ggagcttcat
     6841 cgcccggcct agcgagatca tcagtgcgca gaccgcaagg gcatggcggt agacgaattc
     6901 ggtttcccgc ttgcaccgca tgagcccgtt gaaggcctgg gcattgcgct ggatcgaggc
     6961 gaagatgtca tcgaccaccg gctcaaccgc ctgcggctgg atcgccttgc ccagtcgcgc
     7021 ttccaggaag gcgcggctga tcgtgcgccg gcccttttcg gccacccgcg tggccaggcc
     7081 gaattcctgg accatggtgc gcggggcaac gctgcgcaat gcggcatcgc gttcggccgt
     7141 gcggctggcg cccaagcgtg cggcggcggc cctgcgggct ccgcgctgaa gcgccattgg
     7201 cgcggctggc gccgccttgt gctgcggtgc ggccggaatg gctgccgtat ccagcccctt
     7261 ggccgtgtcg atgacgacgc cgggcacggc gctgcgctgc agctggctca acaggtgctc
     7321 gtcttgcagc aggaaccgcg atttccagaa cgggtggctg aaccagttgc cctccagctt
     7381 gtggatgaac atgccaagtt ccacgtcacc ggttgcgatc ctcttgagca ttgccccctc
     7441 actcccctta agtgcttgag caatctaacc aagacccctt tgccgcaggc aaaacgatcc
     7501 cccggggcaa atacggtgcc gccgggtaag cctctggaaa ggcggcgatc cggcaaaggt
     7561 taagccgcgg ttagccctgt tgcggaagag ggctgcaacc tcgttccggc tagtcccctc
     7621 atgaccgcat ggcgcacggg gcgtgaagcg gggcgcaagg ggcaaggcgc atggacgatc
     7681 cggtgaggcc cggaacaggt acggatcccg agactgacct gcgcgccgcg ccgcgcgtca
     7741 gtctgctgat ccgcccggcc aagctgatcg cgccgtccgg cgaatacctg tgcgtcctgc
     7801 gcgatgcgtc ggccactggg gtcaaggtgc gctgcttcca tccgctgccg gcccagtcca
     7861 tggtcctcga actcggcaat ggcgatcgct acgacgtgga gcggatgtgg gagcagggcg
     7921 atcacgccgg cctgcgcttc cgccaggcga ttccggtcga atacctcatt cgcgaaaccg
     7981 ggccctatcc caagcgcccg gtccgtctgc gcctcaccct gtccgccctg cttgtggcgg
     8041 gccgcgacgt gctgcgcgcc gcggtgctgg acttgtcgca gcagggagcg cgaatcgaat
     8101 gcggtgagca actcgctcgc gatcgccagt tccgcctcga aattcccggg ctcgccccga
     8161 tcgttgccaa ggttcgctgg cgccgcccgc ccgccgccgg cctggtgttc gaggagacgt
     8221 tccggctgga agacttcgcc cgcctcgcat tccgcctcca gcctctgccc gagccgcccg
     8281 gcgaggatga cgacaatccc ttcaagcgtt tggcctgaga tcggcgatac cgggttccac
     8341 tcccaccggg gagggga
//
LOCUS       DATNBV010000199         4944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_764700,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000199 DATNBV010000000
VERSION     DATNBV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764700"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..2480
                     /locus_tag="VN627_04860"
     CDS             219..2480
                     /locus_tag="VN627_04860"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HXD02621.1"
                     /translation="MTEESNVRFTEREALFYHKTIRPGKIEIIASKPMATQRDLSLAY
                     SPGVAVPVQAIADDPATAYDYTAKGNLVAVITNGTAILGMGNLGALASKPVMEGKAVL
                     FKRFADVDSIDIELDSEDPQALIEAIAMMEPSFGGINLEDIKSPECFIIEQALRERMK
                     IPVMHDDQHGTAIIAAAGLLNACHLTGRKFEDVKVVVNGAGASALACTALIKSMGVRH
                     ENVTVCDTKGVIYQGRDHVDQFKSAHAIMTDKRTLKEALVGADIFLGLSAKGAVTQDM
                     VKGMAPQPIIFAMANPDPEITPPEAKAVRPDAIVATGRSDYPNQVNNVLGFPFIFRGA
                     LDVRATTINEEMKIAAARAIAELARAQVPEEVAAAYGINHQFGLDYFIPAPFDPRLIE
                     VVSSAVAKAAMETGVAQKPIEDFEAYRHELKARLNPTTSVLTTIYQQVKEHPKRVVFA
                     EAENEVVLRAAIQFRDFGYGTPVLVGRDQIVRDKLVELGVEHPQSYEIHNSAISSHVP
                     AMVDLLYDKLQRRGFLHRDVQRMVNQDRNVFAAALVKLGVADAMITGMTRTFSQSMKE
                     VRQVLDPKPGHTPFGVHMMVAKNYTVFLADTTVNERPSAEELAHIAVETAAVARRMGH
                     DPRVAFLSFSTFGNPAGKWLDPIRGAVALLDEMQPGFEYEGDIAPDAALNPKVMANYP
                     FSRLSGPANVLVMPGLQSANISAKLLRELAGNAVIGPMLIGMEKPVQIAPMTALAPDI
                     LTLAVLAAGGVVG"
     regulatory      2519..2620
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            2715..3287
                     /locus_tag="VN627_04865"
     CDS             2715..3287
                     /locus_tag="VN627_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HXD02622.1"
                     /translation="MQALLTSTAVVALAEIGDKTQLLAILLATRFKRPWPIIAGIFAA
                     TIVNHFFAALIGSEVASFLDGKWFRYAVAISFLAMAAWTLVPDKLDDEEEVKPARFGV
                     FVTTVISFFLVEMGDKTQIATIALGARYHDAVQVALGTTVGMMIANVPAVFLGNELIR
                     RVPLNVVRMVTAALFAVIGAWLLLQTAGLV"
     gene            complement(3384..4148)
                     /locus_tag="VN627_04870"
     CDS             complement(3384..4148)
                     /locus_tag="VN627_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXD02623.1"
                     /translation="MDLAGFDLAALLPFIAVGFAAQLVDGALGMAFGIISNTALVAVL
                     GVPPAIASQRIHVVECFTTAMSGLSHLYHRNIDKRLFLRLLVPGMAGGVAGAYLLTSL
                     DAGVVKPFVLLYLAGIGIYLLVRGLLYPPQVREARHIAPLGLVGGFLDAAGGGGWGPV
                     VTSNLLIQGADPRKVVGTVNAVEFFLTLTVSAAFIWRLGFADLAGATLGILIGGVLAA
                     PIGAWGARHLPAKLMLIMVGVVLTLTSVYGVYRAWG"
     gene            complement(4244..>4944)
                     /locus_tag="VN627_04875"
     CDS             complement(4244..>4944)
                     /locus_tag="VN627_04875"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HXD02624.1"
                     /translation="TALKEGDPTTLNRLYGRSSSHKLRKGQSELVETLLPAIAVPDEG
                     EISALSLFGDDRPLHLEIGFGGGEHLAHRADLLPDHGFIGCEPFLNGVAQALGHIRDQ
                     HLANVRIHMGDALDVLRRVPDQSLTMVYLLHPDPWPKARHAKRRMMNDGPVNMFAAKL
                     KPGGEFRFGTDHAVYLRHALMVMRRHRDQFEWLCDKPRDFQVRPGGWPETRYEHKART
                     VYGHEVWYFRYRRK"
BASE COUNT          903 a         1766 c         1514 g          761 t
ORIGIN      
        1 caggcagtcc gccgcctcgg ccttgaggcc aaggtactgc gccatcatcg gcgtggtccc
       61 ggcggggatg gccggaccct cagggcggac tgcggcatgc atggagcgag ggcttagccc
      121 cccccgcagc gattcgacaa acacccgacc cgcacctttc ccctatcgtc tggcgccaag
      181 ccgcgttagg gaacccgcga aagcacaggg aggccagcgt gaccgaggaa agcaacgtcc
      241 gttttaccga gcgcgaggcg ctgttctatc acaagacgat ccgtcccggt aagatcgaga
      301 tcatcgcctc caagccgatg gcgacccagc gcgacctgtc gctcgcctat tcccccggcg
      361 tcgccgtccc tgtccaggcc atcgccgacg atccggccac cgcctacgac tataccgcca
      421 agggcaacct cgtcgccgtc atcaccaacg gcacggcgat cctgggcatg ggcaatctcg
      481 gcgcgcttgc ctcgaagccg gtgatggaag gcaaggccgt cctgttcaag cgcttcgccg
      541 acgtcgattc catcgacatc gagctcgaca gcgaagatcc gcaggccctg atcgaagcca
      601 tcgccatgat ggagccgagc ttcggcggca tcaacctaga agacatcaag tcgcccgagt
      661 gcttcatcat cgagcaggcc ctgcgtgagc ggatgaagat cccggtcatg cacgacgacc
      721 agcacggcac cgcgatcatc gccgcggccg gcctgctcaa cgcctgccac ctcaccggcc
      781 gcaagttcga ggacgtcaag gtcgtggtca acggcgccgg cgcctcggcg ctggcctgca
      841 cggccctgat caagtcgatg ggcgtgcgcc acgagaacgt caccgtctgc gataccaagg
      901 gcgtgatcta ccagggccgc gaccacgtcg accagttcaa gtcggcccac gccatcatga
      961 ccgacaagcg caccctcaag gaagccctcg tcggcgccga catcttcctc ggcctctcgg
     1021 ccaagggcgc ggtgacccag gacatggtca agggcatggc cccgcagccg atcatcttcg
     1081 ccatggctaa ccccgatccg gagatcaccc cgcccgaggc gaaggccgtc cgtcccgatg
     1141 ccatcgtcgc caccggccgg tcggactatc ccaaccaggt caacaatgtc ctcggcttcc
     1201 ccttcatctt ccgcggcgcg ctcgatgtgc gcgccaccac catcaacgag gaaatgaaga
     1261 tcgccgccgc ccgcgccatc gccgagctgg cccgcgccca ggtgcccgag gaagtggccg
     1321 ccgcctatgg catcaaccac cagttcgggc tggactactt catccccgca ccgttcgatc
     1381 cgcgcctgat cgaagtcgtc tcctcggccg tcgccaaggc cgcgatggaa accggtgtgg
     1441 cgcaaaagcc gatcgaggat ttcgaggcct accgccacga actcaaggcc cgcctcaacc
     1501 cgaccacctc ggtgctcacc acgatctacc agcaggtcaa ggaacatccc aagcgggtgg
     1561 tcttcgccga ggcggaaaac gaagtggtcc tgcgcgccgc gatccagttc cgcgatttcg
     1621 gctatggcac gccggtcctc gtcgggcgcg accagatcgt gcgcgacaag ctggtggaac
     1681 tcggcgtcga gcatccgcaa agctacgaga tccacaattc ggcgatctcc agccacgtgc
     1741 cggccatggt cgacctgctc tacgacaagc tccagcgccg cggcttcctc catcgcgacg
     1801 tgcagcgcat ggtcaatcag gaccgcaatg tcttcgccgc cgccctcgtc aagctgggcg
     1861 tggccgatgc gatgatcacc ggcatgaccc gcaccttctc gcagtcgatg aaggaagtgc
     1921 gccaggtcct cgatcccaag cccggccaca cgccgttcgg cgtccacatg atggtcgcca
     1981 agaactacac cgtgttcctc gccgatacga ccgtgaacga gcggccgagc gccgaggaac
     2041 tggcccatat cgccgtggaa accgccgccg tcgcccgccg catgggccac gatccgcgcg
     2101 tcgccttcct ctcgttctcg accttcggca atcccgccgg caagtggctc gacccgatcc
     2161 gcggggccgt ggccctgctc gacgagatgc agcccggctt cgaatacgaa ggcgatatcg
     2221 cccccgatgc cgcgctcaac cccaaggtca tggccaacta tccgttcagc cgcctgtcgg
     2281 gtccggccaa cgtgctggtc atgccggggc tgcagtcggc caatatctcg gccaagctgc
     2341 tgcgcgagct cgccggcaac gccgtcatcg gcccaatgct gatcggcatg gaaaagcccg
     2401 tgcagatcgc accgatgacc gcgctcgcgc cggacatcct gaccctggcc gtgctcgccg
     2461 ccggcggcgt ggtcggctga gagaagtgct tgatccacaa tgacgggcag gctaacgccc
     2521 gcccgtcatc tggggagtag ccagccgcgc cgccgcgcgg gcccatgcgt caacatactc
     2581 ggccgagagg ccgtggcgca cgggggatgg gaaaccatcc gggcgagacc aatggcgccg
     2641 aacgcttcgc ctgtgccggg ccgaagcgcc ggcgtccgtt gtgtttcgca tcaaggcccg
     2701 gccggagaag ttcattgcaa gctctgctca cctccaccgc cgtggtcgcc ctcgccgaga
     2761 tcggcgacaa gacccagctc ctcgccatcc tcctggccac ccgcttcaag cgcccctggc
     2821 cgatcatcgc cggcatcttc gccgccacca tcgtcaacca tttcttcgcc gccctgatcg
     2881 gcagcgaggt cgcctcgttc ctcgacggca agtggttccg ctatgcggtc gcgatctcgt
     2941 tcctcgccat ggccgcctgg accctcgtgc ccgacaagct ggacgacgag gaagaggtga
     3001 agcccgcccg cttcggcgtc tttgtcacca cggtgatctc gttcttcctc gtcgagatgg
     3061 gcgacaagac ccagatcgcc acgatcgcgc tcggcgcgcg ctatcacgat gccgtgcagg
     3121 tcgcgctcgg caccacggtc ggcatgatga tcgccaacgt gcccgccgtg ttcctcggca
     3181 acgaactgat ccgccgcgtg cccttgaacg tcgtgcgcat ggtcaccgcc gccctgttcg
     3241 cggttatcgg cgcctggctt ctcctgcaaa ccgcaggcct cgtctgaagc caatgctcct
     3301 tccccctcga tggggggagg atgcgcggac taggcagctt gctgcctagt cgcagctgga
     3361 ggggggtaag ggcgccaacc gccctagccc caagcccgat aaaccccata gacactcgtc
     3421 agcgtcagca ccacgccaac catgatcagc atcagcttgg ccggcaggtg cctggctccc
     3481 caggccccga tcggcgcggc cagcacgcca ccgatgagga tgcccagcgt cgccccggca
     3541 aggtcggcaa agcccagccg ccagatgaag gcggccgaga cggtcagcgt caggaagaac
     3601 tccaccgcat tgaccgtgcc caccaccttg cggggatcgg cgccctggat cagcaggttg
     3661 gaggtcacca ccggccccca gccgccgccg cccgccgcat cgaggaaccc gccgaccagc
     3721 cccagcggcg cgatgtgcct cgcctcgcgc acctgcggcg gatagagcag cccgcgcacc
     3781 agcaggtaga tgccgatgcc ggccaggtag agcagcacga aaggcttcac cacgccggcg
     3841 tcgagcgagg tcagcaggta ggcccccgcc accccgccgg ccatgcccgg caccagcagc
     3901 cgcaggaaca gccgcttgtc gatattgcgg tggtacaggt ggctcagccc cgacatcgcc
     3961 gtggtgaagc attcgaccac atggattcgc tgcgaggcaa tggccggcgg cacgccgagc
     4021 acggcgacca gggccgtatt ggaaatgatc ccgaaagcca tgccgagcgc gccgtccacc
     4081 agctgcgcgg caaagcccac cgcgatgaac ggcagcaagg cggccaggtc gaagcccgca
     4141 aggtccatga tcgaatcgcc ccatcaatct ccaccgcaat gatagagata aatgtggcag
     4201 gagcgaggaa cgatggtttg gccggcctaa actaccgctt ggctcacttg cgccgatagc
     4261 ggaagtacca gacctcgtgg ccgtaaaccg tgcgggcctt gtgctcgtag cgcgtctccg
     4321 gccagccgcc ggggcgcacc tggaaatcgc gaggcttgtc gcacagccat tcgaactggt
     4381 cgcggtggcg gcgcatgacc atcagggcat ggcgcaggta gaccgcatgg tcggtgccga
     4441 agcgaaactc gccgcccggc ttgagcttgg cggcaaacat gttgaccggc ccgtcgttca
     4501 tcatccgccg cttggcatgg cgtgccttgg gccagggatc gggatgcagg agatagacca
     4561 tcgtcagcga ctggtcgggc acgcggcgca ggacgtccaa cgcgtcaccc atgtggatgc
     4621 ggacgttggc gagatgctgg tcgcggatgt ggccgagcgc ctgcgcgacg ccgttgagga
     4681 acggctcgca accgatgaag ccatggtccg gcaaaaggtc cgcgcggtgg gcgagatgtt
     4741 cccctccacc gaagccgatc tccaggtgga gcggccggtc gtcaccgaac aggctcaagg
     4801 cgctgatctc gccttcatcc ggcacagcaa tggccggaag gagggtctcg accagctccg
     4861 actggccctt gcgcagcttg tggctgctcg accggccata gagccggttg agggtcgtcg
     4921 gatcgccttc cttgagcgca gtca
//
LOCUS       DATNBV010000200         3652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1387223,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000200 DATNBV010000000
VERSION     DATNBV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387223"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..558
                     /locus_tag="VN627_04880"
     CDS             304..558
                     /locus_tag="VN627_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HXD02625.1"
                     /translation="MLHFELVTPARLVRSENVYMVVVPGSEGEFGVLEGHAPFMSTVK
                     DGVLKVYRTEGGAPDEIVVAGGFAEVGASGLTVLAEHVEG"
     gene            684..2189
                     /locus_tag="VN627_04885"
     CDS             684..2189
                     /locus_tag="VN627_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02626.1"
                     /translation="MNSTGRGELIPRMLVGISAYRIHAVAFFAAITLVTRCSILGDWN
                     HEIDVQFYMLAGRRLLDGAVLYVDVWDRKPPTLYLTYAILTAFPDPFLAVQLAGAVCT
                     VLASYGLYRIAWTYTNPQGSLLAGAAYAVLTMQFGGGTAEVPILFAPLMVAGAWSLVT
                     ALPGLSRGAVPPRIFAGIACAGLALSFKQSALLECLAFGIAVIGLAWRGGAEKCYLVK
                     TALLLSFTGALPLLAVAMWYWGTGHFAVLWQSLVLSNTTRLFDTGAGMVARYAILAEL
                     LAPIGLFAVLGARAMRPLREAPKPYFLALGWLAAASAALLSYPGLYLHYALPLLLPLC
                     LVAAPFFARRDIGLAAFGLLSVATLIHAGAFNFAERARSRAASAQIVEYLRRETPQHR
                     LLVLNSSPWLYREVHSVPPPTALLFPPHLYDGAEAGASGLDTSAELRRVLDWRPEAVV
                     IQVPLPAQPLNQATVAMVDTYVHRCKRLRQFTLHDHNGPQVQAVYTGCAAV"
     gene            complement(2186..2644)
                     /locus_tag="VN627_04890"
     CDS             complement(2186..2644)
                     /locus_tag="VN627_04890"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HXD02627.1"
                     /translation="MRIVLPIAALSLMLASPVAAASTLPGWLAGTWARENGAAWAEEL
                     WTAPRHGQMLGLFREGFGPDVNTWAYLRIEADRDGNPVLIMHKSGEAAIRYPLAVASE
                     AAIEFASAGQPYPQRIRYWREGQLLMIEISRMDGGETKRFNYRPVSTFQD"
     gene            complement(2685..>3652)
                     /locus_tag="VN627_04895"
     CDS             complement(2685..>3652)
                     /locus_tag="VN627_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXD02628.1"
                     /translation="PRFTTPLNVMLGFLAVMGVMRFDGYATAATRGSYDRAADGVPFN
                     SAVAATGHEAVPADRRNLVIILVEALGRPVSPAASALFESDWNRPEWRTRYDVRTGDV
                     RYFGSTTSGEMRELCSRWKLDETIPFGPTECLPQRLRAAGYETTALHGFSGDMFDRRN
                     WWTKAGFDEVDFGPQLKQAGAHSCGGVFPGACDTDVPSQIAAHLKRSAVRGGHPQLVY
                     WVTLNSHLPVIEDASLGTVDCQLGGEALAAESPLLCPLFTVHHRLANAISRMAMDPAL
                     PPTDILIVGDHMPPFFAREARMNFDPAHVPWVLLKARPAKAHAPS"
BASE COUNT          621 a         1141 c         1182 g          708 t
ORIGIN      
        1 gtccgctcgt tcaaggcggt ggtcgacggc gaatacgacc acctgcccga agccgcgttc
       61 tacatggtcg gcggcatcga cgaagcggtc gagaaggcca agaagctggc tgaagaagcg
      121 taagcggatc gccccgcccg catgggcggg gtaccccaat ctcccctccc gcccgcggga
      181 ggggtcgggg gagggcctgt tgggcggcct tctctgacac gatgacaagc cctcccccag
      241 ccccttccgc aagcgggagg ggagtgagaa gaacctcctg cgatgcggag gtggagactg
      301 gttatgctgc acttcgaact cgtcaccccc gcccggctcg tccgcagcga gaacgtctac
      361 atggtggtcg tccccggcag cgaaggcgag ttcggcgtgc tggaaggcca cgcgccgttc
      421 atgtcgaccg tgaaggacgg cgtcctcaag gtttaccgga ccgagggcgg cgcgcctgat
      481 gaaatcgtag tggccggcgg ctttgccgag gtcggcgcca gcgggctgac ggttctggcc
      541 gaacacgtcg agggctgagc cccaccttcc tgcgtacaat gcagtgaaca ggagcacctg
      601 gcggcttgcc gccgggcgct cgttcgttat gccctgccaa cacggatgaa ttgcccagac
      661 tccccgttgc agagtagcga aaaatgaatt cgactggccg aggagagctg atcccaagga
      721 tgctcgtcgg gatttcagcc taccggatcc atgccgtcgc ctttttcgcc gcgataaccc
      781 tggtgacgcg ctgttcaata ttgggggact ggaatcacga gatcgatgtc cagttctata
      841 tgcttgcggg acgacggctg ctggacggag cggtcctgta tgtcgacgtt tgggatcgca
      901 aaccgccaac cctttatctg acatacgcga ttctcaccgc gttccccgat ccattcctcg
      961 ccgttcaact tgccggcgcg gtctgcaccg ttctagcctc ttacggtctt taccggattg
     1021 catggaccta cacgaacccg caaggcagcc tcctcgcggg tgccgcatac gctgttctga
     1081 caatgcagtt cggtggcgga accgctgaag tgccgatcct gttcgctccg ttgatggtcg
     1141 cgggtgcatg gtcactggtg acagctcttc cgggcctttc tcgcggcgct gtgcccccaa
     1201 gaatttttgc cgggatcgcc tgcgccggac tggccctttc gttcaagcaa tctgccctgt
     1261 tggaatgcct tgcattcgga atcgcggtga taggtctagc ctggcggggg ggcgctgaaa
     1321 aatgctacct cgtgaagact gctttgctgc tttccttcac gggagcgctg ccgctgctgg
     1381 cggtcgcgat gtggtactgg ggcacgggcc atttcgctgt cctttggcag tcactcgtcc
     1441 tgtccaacac gacacgattg tttgacactg gcgcgggcat ggtggcccgc tatgccatcc
     1501 tcgctgagtt gcttgcaccg atcggattgt tcgctgtgct gggcgcgcgg gccatgcgcc
     1561 cgcttcgtga ggcccccaag ccatatttcc ttgcccttgg ctggctggcc gcagccagtg
     1621 ctgccttgct ctcttatccc gggctctatc tgcactatgc cctgcccctg ttgctgccac
     1681 tctgccttgt cgccgcaccg ttcttcgcac gcagggacat cggacttgct gcattcggcc
     1741 tgctttcagt agcaacgctg atccatgccg gcgcattcaa cttcgctgag cgcgcgcgtt
     1801 cacgcgcagc ttcggcgcag atcgtcgaat atctccgcag ggaaacaccg caacatcggc
     1861 tgctcgtttt gaacagttcg ccttggcttt acagggaagt gcactccgtg ccgccgccca
     1921 cggcgctgct gttcccaccc catctttacg acggggcaga ggcaggggcc agcggccttg
     1981 acacttctgc cgagcttcgg cgagtgctgg attggaggcc tgaagccgtc gtgatccagg
     2041 tgccgttgcc ggctcaaccg ctcaatcagg cgacagtagc tatggtcgac acctatgttc
     2101 accgatgcaa acggctacgg caatttacgc tccacgatca taatggaccg caggttcaag
     2161 cggtctatac cggctgcgcg gcagtttagt cctgaaaagt agacaccggc cggtaattga
     2221 accgcttggt ctccccgcca tccatgcggg atatctctat catcagcagt tgtccctcac
     2281 gccagtagcg gatgcgttgg ggatagggtt ggccggcact ggcaaactcg atcgctgcct
     2341 cgctcgcaac ggcaagcggg taccggatcg ccgcttctcc gctcttgtgc atgatgagca
     2401 cagggttgcc gtcgcggtct gcctctattc tcaggtaagc ccaggtgttg acgtccggac
     2461 cgaatccttc gcggaacagg cccagcatct ggccgtgacg gggggcagtc cacagctcct
     2521 ccgcccaggc ggcgccattc tcgcgcgccc aggtgccggc gagccagccc gggagggtgc
     2581 tggcggcggc cacggggctg gcgagcatca gggaaagggc ggcaatgggc aggacgatgc
     2641 gcattcggga tgcttagccc agcctgcgcc gcggcccaag cggttcagct gggcgcgtga
     2701 gccttggccg ggcgcgcctt gaggagaacc cagggcacgt gggccggatc gaagttcatc
     2761 cgggcttcgc gggcgaagaa cggcggcatg tgatcgccga cgatgaggat gtcggtgggc
     2821 ggcagggccg ggtccatggc catccgggag atggcattgg cgaggcggtg gtggactgtg
     2881 aacaaggggc aaagcagcgg cgattcggcg gcgagcgcct ctcccccgag ctggcagtcg
     2941 acagtaccga gactggcgtc ctcgatcacc ggaaggtgcg aattgagcgt cacccagtag
     3001 accagctgcg gatggccgcc gcgcacggca ctgcgcttga gatgggcggc gatctgcgag
     3061 ggaacatcgg tgtcgcaagc gccgggaaac acgccgccgc aggaatgcgc cccggcctgt
     3121 ttcagctgag gaccgaagtc gacctcatcg aagcctgcct tggtccacca gttgcggcga
     3181 tcgaacatat ctcccgagaa accgtgtagt gcggttgtct cgtagccggc ggcgcgcagg
     3241 cgttggggca ggcattcggt ggggccgaac ggaatggttt cgtccagctt ccaccggctg
     3301 cacagttccc gcatctcgcc gctggtggtc gagccgaagt agcgcacatc gccggtgcgg
     3361 acatcgtagc gggtgcgcca ttcgggtcga ttccaatcgg attcgaacag cgccgaggcg
     3421 gcgggtgaga ccggacggcc aagcgcttcg accagaatga tgacgaggtt gcgacgatcc
     3481 gccggcaccg cttcgtgccc cgttgccgcc actgccgagt tgaacggcac cccatcggcg
     3541 gcacggtcat agctgccgcg ggtggcggct gtcgcatagc catcgaagcg cattacgccc
     3601 atcaccgcga ggaacccgag catgacgttc aacggcgtgg tgaaccgtgg ca
//
LOCUS       DATNBV010000201         4542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2675076,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000201 DATNBV010000000
VERSION     DATNBV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2675076"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /locus_tag="VN627_04900"
     CDS             complement(<1..1508)
                     /locus_tag="VN627_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXD02629.1"
                     /translation="MHSSIKISASILALASAGLAAPAFAQAAPAAAEGTTASETTGLT
                     DIVVTATKRETNLQKTPIAISVLGADLVEKRHVQSLIDLADGGVPSLRVATFEARQSA
                     LTIGIRGIVPFDQNQVAREPGVGVYVDGVYLGRSQGLNAALFDVQRIEVLRGPQGTLF
                     GRNTEGGALSIVTKTPTGEFGGRVSAGVGNYGSRDGEIHLNLPAFANFAIKVDGIYDH
                     QDATVKNPASGQYGWNYHDRVGGRIAGRWTPVDGLTVDVAYDQAKDENTPFYSQLVNY
                     NPLNKTIGTYIVDPSLVNPNLTSSSSYILVAPGTTTKCSSCIAPLAPLVTVSGSNRMS
                     VADLGVTQQPSVDETHGFNAAIKYKLSPEIELRSITAWRGVETHQWDGSAGAHRSVFV
                     PYGEFGRYSLSELFQHQFSQELQVVGTLPHLDFVLGGYYFNEHVQERAATPNPLKWNL
                     DGTGYTFINEVPANPTGAISSSNQGWDRRYWYIQRDSQAVGKSYGVFGQATWT"
     gene            1765..2118
                     /locus_tag="VN627_04905"
     CDS             1765..2118
                     /locus_tag="VN627_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXD02630.1"
                     /translation="MRDHSPAPTDHALPGETRYRHGELIVDPVAFAVRFRGKVLPLRP
                     NEFRLLAFFVAHPDRVFSRTALIANLGKDSDMIDERTVDVWTGRLRRALREHGVPDPL
                     RTVRAQGYVLDSISE"
     gene            complement(2128..2820)
                     /locus_tag="VN627_04910"
     CDS             complement(2128..2820)
                     /locus_tag="VN627_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02631.1"
                     /translation="MISPRFRAGALCAIAAGLLASPVSAGLMLSKVILDLGPESAPRD
                     DIELWNDGAERLYVVAEPSEIADPGLPTQLRKPSPDPAVTGLLVTPQKLILEPGEHKL
                     VRIAAVAARPARDRVYRVTIKPVAGPVSSDQSALKLFVGYDALVLYRPEQPRADLAAT
                     RNGRQLTIHNGGNTNVELAQGRQCDAGGQDCRDLPANRLYPGADWTIALPFTTPVTYR
                     TWTGRTEGTAKF"
     gene            complement(2817..3287)
                     /locus_tag="VN627_04915"
     CDS             complement(2817..3287)
                     /locus_tag="VN627_04915"
                     /inference="COORDINATES: protein motif:HMM:NF027306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HXD02632.1"
                     /translation="MPRAALRGGAGLTIDLPAYRSYDVRLRAAAGSALGYDGATRRVT
                     LFPGNVAAFSWQTRPQLAIYARLVAPDGAPLANAALSAGSNIAHTAADGGFMLQLDAE
                     RTITAHLPDGTTCTAQVPDSAEGDASHGFASLKALVCERQTMPSLFAKHREQQP"
     gene            complement(3251..>4542)
                     /locus_tag="VN627_04920"
     CDS             complement(3251..>4542)
                     /locus_tag="VN627_04920"
                     /inference="COORDINATES: protein motif:HMM:NF028277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HXD02633.1"
                     /translation="LDERSRVDAYVGGRLVSSGLFEAGNQALDTSGMPEGSYEVVLRI
                     AAARGGIREERRQFTRSPAIPPLGHDAWGLTAGALVDEGRGGSLLGTVTRTPYVQASW
                     AHRLGPHWALGLGVLGTDQRQMVQARAMLAGRRLIGDASLLVSAKGDYGAFARIGSQP
                     GGRLSFNFDARLVTTRDGRPLLPAATPITVWAGPQKQFGQGPLASAPFGQGSRHEAEV
                     SGDVAWQLGRSRIALVGSWRKVDGQSQYAIGPSLRVPLLRARDFEVVGRADYAETERG
                     HSGFVGISLMLTRGNKSFSAESGLSRSAGTAGGPSATGSLRGTISGETGLGEVQGSAA
                     LTRDEQASYASADVTLRGRRGELAAGIGQALGGVGGTQYALSLRTAVAAAGGHIALGR
                     IDGGEARIMARVEARRANASSCWSTTCPAPRCAAGRA"
BASE COUNT          687 a         1457 c         1672 g          726 t
ORIGIN      
        1 gtccaggtcg cctggccgaa gacgccgtag ctcttgccca cggcctggct gtcgcgctgg
       61 atgtaccagt acctgcggtc ccagccctgg ttggacgagg agatcgcgcc ggtcggattg
      121 gcggggacct cgttgatgaa agtgtagccg gtgccgtcaa ggttccactt gagcgggttg
      181 ggcgtcgcgg cccgctcctg aacgtgttcg ttgaagtagt agccgcccag cacgaaatcg
      241 aggtgcggca gggtgccgac aacctgaagc tcctggctga actggtgctg gaacagttcc
      301 gagaggctgt agcggccgaa ctcgccgtag ggaacgaaga cgctgcgatg ggcgccggcc
      361 gagccgtccc actggtgggt ttccacgccg cgccaggcgg tgatcgagcg cagttcgatt
      421 tccgggctca gcttgtactt gatcgccgcg ttgaacccgt gggtctcgtc gacgctgggc
      481 tgctgggtca cgcccagatc ggccaccgac atgcggttgg agccagacac cgtcacgagc
      541 ggggcgagcg gcgcgatgca cgacgagcac ttggtcgtgg tgcccggcgc gacgaggata
      601 tacgagctcg acgaggtcag gttggggttc accaggctcg ggtcgacgat ataggtgccg
      661 atcgtcttgt tcagcggatt gtagttgacc agctggctgt agaacggggt gttctcgtcc
      721 ttcgcctggt cgtaggcgac gtccaccgtc agtccgtcga ccggggtcca gcggccggcg
      781 atgcggccgc cgacgcgatc atggtagttc cagccgtact ggccgctggc ggggttcttc
      841 accgtcgcgt cctggtggtc gtagatgccg tcgaccttga tggcgaagtt ggcaaaggcc
      901 ggcaggttga ggtggatttc gccatcgcgc gaaccatagt tgcccacgcc ggccgagacg
      961 cggccgccga attcgccggt gggggtcttg gtcacgatcg agagcgcgcc gccttcggtg
     1021 ttgcggccga acagggtgcc ctgcggcccg cgcaggactt cgatgcgctg cacgtcgaac
     1081 agggcggcat tgaggccctg cgaacggccg aggtagacgc cgtcgacata gacgccgacg
     1141 cccggttcgc gggcgacctg gttctggtcg aagggaacga tgccgcgaat gccgatggtc
     1201 agggctgact ggcgggcttc gaaggtggcg acacggagcg agggaacgcc gccgtcggcc
     1261 aggtcgatca ggctctggac gtggcgcttc tcgaccaggt cggcaccgag gaccgagatg
     1321 gcgatcgggg tcttctggag gttggtttcg cgcttggtgg cggtgacgac gatgtcggtc
     1381 aggccggtcg tctcgctggc ggtggtgcct tcagcggcgg cgggagcagc ctgcgcgaag
     1441 gcgggagcgg caagtccagc gctggcgagc gcgaggatcg aggcggaaat cttgatcgag
     1501 ctgtgcatga tgcgttccct tccgggcctt tcgttcagtg gccggcggga agacgaaccc
     1561 tcatggtcga ttcggctgag ccggaccccc gttggccgag gcccggctag gccagccgag
     1621 ttgcagttcg tggacagttc gatgaccgga atatgacacg gacggctaga ctttgctgca
     1681 atgcggaatg tcaggaaacc gtcacgatat cgtcatgcgg gcgctgcatc ggggtcgcag
     1741 tgccattttt cagacagcct gctcatgcgc gaccattctc ctgccccgac cgatcacgcc
     1801 ctgccgggcg agacccgcta ccgccacggc gagcttatcg tcgatccggt ggccttcgcg
     1861 gtgcgttttc gcggcaaggt gctgccattg cggcccaacg aattccgcct gctggccttc
     1921 ttcgttgccc accccgaccg ggtgttcagc cgcaccgcgc tgatcgccaa tctcggcaag
     1981 gacagcgaca tgatcgacga gcgcacggtc gatgtctgga ccggccgcct gcgccgcgcc
     2041 ctgcgcgagc acggggtgcc cgatccgctg cgcacggttc gcgcgcaggg ctatgtactc
     2101 gacagcatca gcgaatagat tggccgctca gaacttcgcc gtgccttcgg tccggccggt
     2161 ccaggtccgg taggtcaccg gggtggtgaa cggcagagcg atggtccagt cggcgccggg
     2221 atagagccgg ttggccggca gatcgcggca atcctgcccg cccgcatcgc actggcgccc
     2281 ctgggcgagc tctacattgg tattgccgcc gttgtggatc gtcagctgcc gcccgttgcg
     2341 ggtggcggca aggtcggccc tgggctgctc ggggcggtag agcaccaggg catcgtagcc
     2401 gacgaacagc ttcagggcgc tctggtcgga cgagaccggc ccggcaaccg gcttgatcgt
     2461 cacccggtag acccggtcgc gcgcggggcg ggcggccacg gcggcaatgc gcaccagctt
     2521 gtgttcgccg ggctcgagga tcagcttctg cggggtgacg agcagaccgg taaccgccgg
     2581 atcgggcgag ggcttgcgca gctgcgtggg caggcccgga tcggcgatct cggaaggttc
     2641 ggccacgaca tagagccgct cggcgccatc gttccacagc tcgatatcgt cgcgcggggc
     2701 gctctccggg ccgaggtcga gaatgacctt gctcagcatc aggccggccg agaccgggct
     2761 ggcaagcagg ccggcggcta tcgcgcagag ggcaccagcg cggaaacggg gtgaaatcat
     2821 ggctgttgtt ccctgtgttt ggcgaacagg cttggcattg tctggcgctc gcagacaagc
     2881 gccttgagcg aggcgaagcc gtggctggcg tcgccttcgg cgctgtcggg cacctgggcg
     2941 gtgcaggtag tgccgtcggg caggtgagcg gtgatggtcc gttcggcatc gagctggagc
     3001 atgaagccgc cgtccgccgc cgtatgggcg atgttgctgc ctgcgctcag cgcggcattg
     3061 gcgagcgggg cgccatcggg ggcgaccagc cgggcataga tcgccagctg cgggcgggtc
     3121 tgccacgaga aggcggcgac attgccgggg aacagggtga cgcgccgggt tgcgccgtcg
     3181 tagccgagcg ccgagccggc agcggcgcgc aggcggacat cgtaggagcg gtaggccggc
     3241 aggtcgatgg tcaggcccgc cccgccgcgc agcgcggcgc ggggcacgtc gttgaccagc
     3301 agctcgaagc gttcgcccgc cgggcctcga cccgggccat gatccgcgcc tcgccgccat
     3361 caatccggcc gagggcaata tgcccgccgg ccgcggcaac cgcggtgcgc agcgacagcg
     3421 catattgcgt gccgcccacc ccgcccagcg cctggccaat cccggcagca agttcgccgc
     3481 ggcggccgcg cagggtgacg tcggccgagg cataggaggc ctgctcgtcg cgggtcagcg
     3541 cggcgctgcc ctggacttcg ccgaggccgg tttcgcccga gatggtgccg cgcaaggagc
     3601 cggtagcgct ggggccaccg gcggtgccgg cggagcggga gagcccgctt tcggcgctga
     3661 acgacttgtt accgcgcgtc agcatcaggc tgatgccgac gaagccggaa tggccgcgct
     3721 cggtctcggc gtagtcagcc cgcccgacga cctcgaaatc ccgcgcgcgc agcaggggca
     3781 cgcgcagact ggggccgatg gcatactggc tctgcccgtc taccttgcgc cagctgccga
     3841 cgagggcaat ccggctccga ccaagctgcc aggcgacatc gccgctgacc tcggcctcgt
     3901 ggcggctgcc ctgtccgaag ggggccgacg cgagcgggcc ttgcccgaat tgcttctgcg
     3961 gtccggccca gaccgtaatc ggcgtggcag cgggcagcag cgggcgtcca tcgcgcgtgg
     4021 tgacgaggcg ggcgtcgaaa ttgaacgaga gccgtccgcc cggctgcgag ccgatccggg
     4081 caaaggcgcc gtaatcgccc ttggccgaaa ccagcaggct ggcatcgccg atgagacggc
     4141 ggccggcgag catggcgcgg gcctggacca tctggcgctg gtcggtgccc agaacgccga
     4201 gacccagcgc ccagtggggg ccgagccggt gggcccagct cgcctggaca tagggcgtgc
     4261 gggtcaccgt gcccagcagc gagccgccgc ggccttcgtc caccagcgca ccggcggtga
     4321 ggccccaggc atcgtggccg agcggcggga tcgccgggct gcgggtgaac tggcggcgct
     4381 cctcgcggat gccgccgcgc gccgcggcga tccgcagcac gacttcgtag gacccttcgg
     4441 gcatgccgct ggtatcgagc gcctgattgc cagcctcgaa caggccggat gaaaccagcc
     4501 gcccgccgac ataggcatcg acccgcgagc gttcgtcgag at
//
LOCUS       DATNBV010000202         7323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2675576,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000202 DATNBV010000000
VERSION     DATNBV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2675576"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VN627_04925"
     CDS             <1..426
                     /locus_tag="VN627_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD02634.1"
                     /translation="VMNCCHAAMPDMVAARHGRIVTITSDSGRVGDPRLAAYAGAKAG
                     AAGFMRSLASEAARYAVTCNNISLATQEPPTWADWTEEQKAEFYRSPQGQTRMARYAI
                     RRFGKPRDVAAMALFLCSDAADWITGQTYPVNGGYSFAI"
     gene            577..996
                     /locus_tag="VN627_04930"
     CDS             577..996
                     /locus_tag="VN627_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02635.1"
                     /translation="MSIFKKATLATVLGATALSAASPAMAQGYYYGHRSGGDSTGAAI
                     AGGIIGLALGAIVASSVNNHHDRYAERGWTWRDGYYWDRDGRRYYRDGRPCEDNRGYR
                     NGYYDRRGYDDRGYGYGDRGYYGRDNYYSGQTSRYGY"
     gene            1197..2369
                     /gene="purT"
                     /locus_tag="VN627_04935"
     CDS             1197..2369
                     /gene="purT"
                     /locus_tag="VN627_04935"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HXD02636.1"
                     /translation="MTFVAKILLLGSGELGREFVISAKRLGCEVIACDAYDNAPAMQL
                     ADGREVFSMLDPLALRAAIERHRPDFVVPEVEAIRTEVLAELEAEGVHIVPSARATQM
                     TMNRDAIREVAAVELGLRTSRYRYAESLDEVRAGAAHTGLPCVIKPVMSSSGKGQSTV
                     RAEADLEAAWNYAVANMRGDRARVIVEEFVAFDYEITLLTVRTREGVLFCPPIGHRQE
                     RGDYQESWQPAAMSPAALAAAQDMARRVVDDLGGYGIFGVEFFVKGEDVIFSELSPRP
                     HDTGMVTLISQGLSEFDLHARAILGLPIPQVTLYGPSASAVILADRHAGELAYAALAE
                     ALATPGGEVDLRIFAKPTARPWRRMGVALARAGTTDEARRVAAEAAGKVRIVYGGE"
     gene            2510..2890
                     /locus_tag="VN627_04940"
     CDS             2510..2890
                     /locus_tag="VN627_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02637.1"
                     /translation="MTLLKKAVLTGALGASALTVASPAMADDWRWRRHHGGDEAGAAV
                     AGGIIGLALGAAIASGSRDRYYDDDYYYRHHRPRARVYVYQSYPRYYGGYDRPYYRHY
                     YPHGYYNGYGGGYYGGYGGYYRGW"
     gene            2933..4111
                     /locus_tag="VN627_04945"
     CDS             2933..4111
                     /locus_tag="VN627_04945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXD02638.1"
                     /translation="MSAAPPNSSAHYADLRIALFSGNYNYVRDGANQALNRLVGYLLR
                     QGAQVRVYSPVVEHPAFPPTGDLVNLSSFAVPGRAEYRIPTHLSRKVRRDLAQFAPNV
                     VHVSSPDPGAHRAVSWARARHLPILASVHTRFETYPRYYNLAFIEPLVEALLRRFYRR
                     CDALVAPSESMAQVLREQRMNYDIDIWSRGVDREIFDPARRDMAWRRQLGIADNEVVV
                     GFFSRLVMEKGLDVFSDAIDELRRRGVAHRVLIVGDGPARDWFGSRLPDAIFAGFQSG
                     DNLARAVASMDVLFFPSITETFGNVTLEAMASGLPVVVAAATGSESLVYDNRSGRLIR
                     PGAIREYADALEAYIADPALRAAHGAAGEARSREFSWDRINQVVADTYLRLIRRNAAR
                     "
     gene            4159..5481
                     /locus_tag="VN627_04950"
     CDS             4159..5481
                     /locus_tag="VN627_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HXD02639.1"
                     /translation="MADLFPEALPPAAPDAPREDAPLADRLRPRDLSEVIGQEHITGP
                     DGAIGRMVAAGRLSSLILWGPPGTGKTSIARLLADAVGMRYHAVSAVFSGVADLKKAF
                     AEADVAARAGQRTLLFVDEIHRFNRAQQDGFLPFVESGTVTLVGATTENPSFELNAAL
                     LSRAQVLILHRLDADALERLLARAEALEGKPLPLDADARAALVASADGDGRFLLNQAE
                     TLWSVNLPEPLDPAGLGKFLQRRVAVYDKDREGHYNLISALHKALRGSDPQAALYYLA
                     RMLTAGEEPLYVLRRLVRFASEDIGLADPQALVQCLAAKDAYDFLGSPEGELAVVQAC
                     LYCATAPKSNAAYAAQKAAWKSARETGSLMPPANILNAPTRLMKQIGYGKDYAYDHDA
                     DDGFSGANYWPEGMAPQAFYRPVERGFEREVLKRLDWWDKKRRERAAE"
     gene            5478..6365
                     /locus_tag="VN627_04955"
     CDS             5478..6365
                     /locus_tag="VN627_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02640.1"
                     /translation="MTPTRFGHFAAIDWSGAAGERHQGIAVALAATGKAAPALVRPGH
                     RWSRPEVLHWLLHDLPPDSLVGLDLGQSLPFADRGAFFPGWSESPPGARELWALVDRV
                     CAADLHLAASSFVDHPEAARHFRRHGGREGDCFGADGKPGGRGRFRLTERAQEAMGCR
                     PYSNFNLVGAAQVGKSSLTGMRLLHRLSGHLPVWPVDPLPPRGSVIVEIYTTIAAMAA
                     GRPAGRSKMRSHADLAEALAALGSAPVPGRGPIADHASDALVTAAWLRQAAHDPALWS
                     PGGLTPELAATEGWTFGAR"
     gene            6403..7215
                     /locus_tag="VN627_04960"
     CDS             6403..7215
                     /locus_tag="VN627_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXD02641.1"
                     /translation="MRTTVNPLRLEHMTVFDLAPRQVIDLAEQLEIPFISLFLSDGLP
                     GARPVTRAEVPDLRNRLRSSPVTLDSAEVVVLSPDRASWQPTLELAAELGARSVVAIH
                     VLPADDAEAAAQLAELAAAAASFDLKVSLEPISMGQVRTPDHGQRLIRQTGAANIGLT
                     LDLLHIVRTGTPLGTLAAIDPAVIFTAQVCDGPATIAPDHAVEEAGYQRGIPGTGVFP
                     VVDFFRALPPDIVIGMEVPLKSQREAGMSALERTRAVLDATRRLQQLALAGG"
BASE COUNT         1130 a         2798 c         2241 g         1154 t
ORIGIN      
        1 gtgatgaact gctgccatgc ggccatgccc gacatggtcg ccgcccgcca tgggcgcatc
       61 gtcacgatca cctcggattc cggccgcgtc ggcgatcccc gcctcgccgc ctatgccggg
      121 gccaaggccg gagcggcagg cttcatgcgc tcgctcgcca gcgaggccgc ccgctacgcc
      181 gtgacctgca acaacatttc cctcgccacc caggagcccc ccacctgggc cgactggacc
      241 gaggagcaga aggcggagtt ctaccgcagc ccccaaggcc agacccgcat ggcgcgctac
      301 gccatccgcc gctttggcaa accgcgcgac gtcgccgcca tggccctttt cctctgctct
      361 gatgccgcgg actggatcac cggtcagacc tacccggtca acggcggata cagcttcgca
      421 atctgacttc ccgttcagtc acgccttaac aaacctgaac gaacgctgcc aagcacactc
      481 gacacgcgtt caggttcgca agcctaaaga tgaactcaag cggcgaatca gccaaggcaa
      541 tcgggcctga cctgagccgc cgcaaggagt tcagccatga gcatcttcaa gaaggcaacg
      601 cttgccaccg tccttggcgc cactgccctg agcgccgcca gcccggccat ggcccagggt
      661 tactactatg gccatcgctc gggcggtgac agcaccggcg cagccattgc cggcggcatc
      721 atcggcctgg ccctcggcgc catcgtcgcc tccagcgtca acaaccacca cgaccgctac
      781 gcagagcgcg gctggacctg gcgcgacggc tactactggg accgcgacgg ccggcgctac
      841 taccgtgacg gccgcccctg cgaggacaac cgcggttatc gcaacggcta ctacgaccgg
      901 cgcggctacg acgatcgcgg ctacggctac ggcgaccgcg gctactacgg gcgtgacaac
      961 tactattcgg gccaaacctc ccgctacggc tactgatctt ccccagaccg accctgcggc
     1021 cgccctcctg cggccaaggg gaagggaggg cgtccggcct gccgccgggc gccctttgcc
     1081 gtgtctgccc tatgcacatt tcgccggtga tagcttgccc gaagcaccgg gccgggcgtg
     1141 gacattgcgc gcgcttgccg tcacctacgc atgaccaaac aagcggggca tgtaacgtga
     1201 ctttcgtggc gaaaattctt ctgctcggct ctggcgaact gggccgcgag ttcgtgatat
     1261 cggccaagcg gctcggctgc gaggtcatcg cctgcgacgc ctatgataat gcgccggcca
     1321 tgcaactggc cgacgggcgc gaagtcttct cgatgcttga tcccctggct ctgcgcgcag
     1381 cgatcgagcg ccaccggccc gatttcgtcg tgcccgaggt cgaagcgatc cggaccgagg
     1441 tcctggccga actcgaagcc gagggggtgc acatcgtgcc gtcggcccgc gccacccaga
     1501 tgacgatgaa ccgcgatgcc atccgcgagg ttgccgcggt cgagctgggc ctgcgcacct
     1561 cgcgttaccg ctatgccgaa agcctggacg aggttcgcgc cggcgccgcg cacaccggcc
     1621 tcccttgcgt gatcaagccg gtcatgtcct cgtcgggcaa gggccagagc acggttcgtg
     1681 cggaggccga cctcgaagcg gcctggaact atgccgtggc caacatgcgc ggcgaccggg
     1741 cgcgggtcat cgtcgaggaa ttcgtcgcct tcgactatga aatcaccctg ctcaccgtcc
     1801 gcacccgcga gggcgtgctg ttctgcccgc ccatcgggca ccgccaggaa cggggcgact
     1861 accaggagag ctggcagccg gccgcaatgt cgcccgcggc ccttgccgcc gcgcaggaca
     1921 tggcccgccg cgtggtcgac gatcttggcg gctacggcat cttcggcgtg gagttcttcg
     1981 tcaagggcga ggacgtgatc ttctccgaac tctccccccg cccccacgac accggcatgg
     2041 tcaccctgat ctcgcagggg ctcagcgagt ttgacctgca tgcccgcgcg atcctcggcc
     2101 tgcccattcc gcaggtgacg ctttatggcc cgtccgcgtc ggcggtgatc ctcgccgatc
     2161 gccatgccgg ggagctggcc tatgccgccc tcgccgaggc cctggccacg ccggggggcg
     2221 aggtcgacct caggatattc gccaagccga ccgcgcgccc gtggcggcgc atgggcgtcg
     2281 ccctggcccg ggccgggaca accgacgagg cccgccgcgt ggcggccgag gcggccggca
     2341 aagtgcggat cgtttacggc ggggaatgag cgccagccgc ctgccgtggc cttgaattca
     2401 gcctgtcgca aaccgtcgca gggttaaccg ggcgttcagc cccaaaatgg ctcaatctct
     2461 cctaaccgcg cggcaggagc gggtttttct aggctggaga gaacccacca tgacccttct
     2521 caagaaagca gtactgaccg gcgcgcttgg cgcctcggcc ctgaccgtag cctcaccggc
     2581 catggccgac gactggcgct ggcgccggca ccacggcggc gacgaagccg gcgcggccgt
     2641 agccggcggc atcatcggcc tggccctggg cgcagccatc gcctcgggca gccgcgaccg
     2701 ctactacgat gacgactatt attatcgtca tcaccggccg cgcgcacggg tctacgtcta
     2761 ccagtcctac ccgcgctact acggcggcta cgaccggccc tactaccgcc attactatcc
     2821 ccacggctac tacaacggct acggcggcgg ctactacggc ggctacggcg gttattaccg
     2881 gggctggtaa ggccctcgcc aaaacctgaa accgggtgta gggggcggcc gcatgtcggc
     2941 ggcccccccg aactcctcag cgcattatgc ggacctccgc atcgccctgt tcagcggtaa
     3001 ctacaattat gtgcgcgacg gggcgaacca ggcgctgaac cggctggtcg gctatcttct
     3061 gcgccagggc gcccaggtcc gggtctattc tccggtcgtc gaacaccccg ccttcccgcc
     3121 gaccggcgat ctcgtcaacc tgtcctcctt cgccgtcccc ggccgggccg agtatcgcat
     3181 cccgacccac ctttcccgca aggtccggcg cgacctggcg cagttcgcgc ctaacgtcgt
     3241 tcacgtctcc tcgcccgatc ccggcgcgca ccgcgccgtc tcctgggccc gcgcacgcca
     3301 cctcccgatc ctcgcgtcgg tccacacccg gttcgagact tacccgcgct actacaatct
     3361 cgccttcatc gagccgctgg tcgaggcgct gctgcgccgg ttctaccgcc gctgcgatgc
     3421 cctcgtcgcc ccgtcggaat cgatggccca ggtcctgcgc gagcagcgca tgaactacga
     3481 tatcgacatc tggtcgcgcg gggttgaccg cgaaatcttt gaccccgccc ggcgcgacat
     3541 ggcctggcgc cgccagctcg gcattgccga caatgaggtc gtcgtcggct tcttcagccg
     3601 cctcgtcatg gaaaaggggc tcgacgtctt ctccgacgcg atcgacgaac tgcggcgccg
     3661 gggcgttgcc caccgcgtcc tgatcgtcgg tgacgggccc gcgcgcgact ggttcgggag
     3721 ccgcttgccc gacgccatct tcgccggctt ccagtcgggc gataacctcg cccgcgcggt
     3781 cgcctcaatg gacgtgctgt tcttcccctc gatcaccgaa accttcggca acgtcacgct
     3841 cgaggccatg gccagcggcc tgcccgtcgt cgtcgccgcc gccaccggca gcgagagcct
     3901 ggtctacgac aaccgctccg gacgcctgat ccgccccggc gcgatccgcg aatatgccga
     3961 tgccctggaa gcctacatcg ccgacccggc cctgcgcgcc gctcacggcg ccgccggcga
     4021 ggcgcgcagc cgcgaattct cgtgggaccg catcaaccag gtcgttgccg acacctacct
     4081 gcgcctgatc cgccgcaacg ccgcgcgctg aacgcggcgg atgcaatctg cgccgcatcg
     4141 tcctatctag cgtctgcgat ggccgacctc ttccccgaag ccctgccccc tgccgcgccc
     4201 gacgccccgc gcgaggatgc gccgctggcc gatcgcctgc gcccgcgcga cctgagcgaa
     4261 gtcatcgggc aagagcatat caccggaccg gacggcgcga tcggccggat ggttgccgcc
     4321 ggacgcctgt cctcgctgat cctgtggggg ccccccggca ccggcaagac ctcgatcgcc
     4381 cgcctcctcg ccgatgccgt gggcatgcgc taccatgcgg tgagcgccgt gttctcgggc
     4441 gtcgccgatc tcaagaaggc cttcgccgaa gccgatgtcg ccgccagggc ggggcagcgc
     4501 acccttctgt tcgtggacga gatccaccgc ttcaaccgcg cccagcaaga cggcttcctg
     4561 cccttcgtgg aaagcggcac ggtcaccctc gtcggtgcta cgaccgaaaa ccccagcttt
     4621 gagctcaacg ccgcgctgct ctcccgcgcc caagtcctga tcctccaccg gctcgatgcc
     4681 gatgcgctgg aacggctgct cgcccgggcc gaggcgctgg agggcaagcc cctccccctg
     4741 gatgccgatg cccgcgccgc gctggtcgcc tcggcggatg gagacggccg cttcctgctc
     4801 aaccaggccg aaaccctgtg gtcggtcaac ctgcccgaac cgctcgatcc agcgggcctc
     4861 ggcaagttcc tccagcgccg tgtcgccgtt tacgacaagg accgggaggg gcattacaat
     4921 ctcatctccg cgctccacaa ggcactgcgc ggctccgacc cgcaggccgc gctctactac
     4981 ctcgcacgca tgctcaccgc gggagaagag ccactctacg tcctgcgccg cctcgtccgc
     5041 ttcgcgagcg aggacatcgg gcttgccgac ccgcaagccc tcgtccagtg cctcgctgcc
     5101 aaggacgctt acgatttcct cggctccccc gagggcgagc tcgccgtagt ccaggcctgc
     5161 ctctattgcg cgacggcccc caagtcgaac gccgcctatg ccgcgcagaa ggccgcgtgg
     5221 aaatcggccc gcgaaaccgg ttcgctgatg cccccggcca atatcctcaa cgcccccacc
     5281 aggctgatga agcagatcgg ctacggcaag gactacgcct acgaccacga cgccgacgac
     5341 ggcttttccg gcgccaacta ctggcccgaa ggcatggccc cgcaagcctt ctaccgcccg
     5401 gtcgagcgcg gcttcgaacg cgaggtgctg aagcggcttg actggtggga caagaagcgc
     5461 cgcgagcgcg cggccgagtg acgccaactc gctttggtca cttcgccgcg atcgactggt
     5521 caggcgccgc gggcgagcgc caccaaggca tcgccgtcgc cctcgccgcc accggcaagg
     5581 cagctcctgc cctcgtccgc cccggccacc gctggtcgcg ccctgaagtg ctgcactggc
     5641 tgctccacga tctcccgccc gacagccttg tcggcctaga cctcggccag tcgctccctt
     5701 ttgccgaccg cggcgccttc ttccccgggt ggagcgagag cccgcccggt gcgcgcgagc
     5761 tgtgggcgct ggtagaccgg gtctgcgccg ccgatcttca cctcgccgcc agcagcttcg
     5821 tcgatcatcc agaggctgcc cgtcacttcc gccgccacgg cggccgcgaa ggcgactgtt
     5881 tcggcgccga tggcaaaccg ggcggccgcg gccgcttccg cctgaccgag cgcgctcagg
     5941 aggccatggg ctgccggccc tattccaatt tcaacctcgt cggcgcggcc caggtcggca
     6001 agtcgagcct caccggcatg cgcctgcttc atcgcctgtc cggccacctc ccggtctggc
     6061 cggtcgatcc gctgccgccc cggggatcgg taatcgtcga aatctacacc accatcgccg
     6121 ccatggccgc cggccgcccc gcagggcgct ccaagatgcg cagccatgcc gatcttgccg
     6181 aggccctcgc cgccctgggc agcgcgccgg tgccgggccg cggccccatt gccgatcacg
     6241 ccagcgacgc gctggtcacc gccgcatggc tccgccaggc cgcccacgat cctgcccttt
     6301 ggtcgcccgg cggcctcacc ccggaactgg ccgcaaccga aggctggacc ttcggcgccc
     6361 gctgagtcac atctgctcgg ctgatcggga aaggatgctg cgttgaggac gaccgtgaac
     6421 cccctgcgcc tcgaacacat gaccgtgttc gatcttgcgc cccgccaggt catcgacctc
     6481 gctgaacagc tcgagattcc gttcatcagc ctgttcctct cggacggact gcccggcgcg
     6541 cgtccggtca cgcgagccga agtccctgac ctgcgaaacc gcctgcgttc gtccccggtc
     6601 accctcgatt ccgccgaagt ggtcgtgctc agccctgatc gcgcgtcgtg gcaacccacg
     6661 ctcgaacttg ccgccgaact cggcgcgcgc agcgtcgtcg cgattcacgt gctgccggcc
     6721 gacgatgccg aggcagcggc gcagctcgcc gaacttgccg cagccgccgc ctcgttcgac
     6781 ctcaaggtct cgctggagcc gatctccatg ggccaggtcc gcacgcccga ccatgggcag
     6841 cgcctgatcc ggcaaaccgg cgccgccaac atcgggctca ctctcgatct cctgcatatc
     6901 gtccgcaccg gaaccccgct cggcacgctc gccgcgatcg accctgcggt gatcttcacc
     6961 gcccaggtct gtgacggccc cgctacgatc gcgcccgatc acgcggtgga ggaagcgggc
     7021 taccagcggg gcatccccgg aaccggcgtg ttcccggtcg tcgacttctt cagggcgctt
     7081 cccccggata tcgtgatcgg gatggaggtc ccgctgaaaa gtcagcgcga agccgggatg
     7141 agcgcgctcg agcgaacccg cgccgtcctc gatgcgaccc gccggctcca gcagctggcc
     7201 cttgccggag gctgacccgg cctcaggccg ccggatgggc caccgcgacc ctcggcacct
     7261 ggccacctct caccagcaag gtcgccacgg ctcccgccac catcatcagg cccgccagca
     7321 cca
//
LOCUS       DATNBV010000203         3537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3310998,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000203 DATNBV010000000
VERSION     DATNBV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3310998"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VN627_04965"
     CDS             <1..322
                     /locus_tag="VN627_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HXD02642.1"
                     /translation="FECAGAYGSLDQAISAVRRGGTVAAPGFHWGQDKFFGMGALIKE
                     VTIRYTNMYNTAEFARAVDTLDLGHVEPRAMITSTVDLAGAPAAFEELRGANTQCKVM
                     IRPW"
     gene            373..1314
                     /locus_tag="VN627_04970"
     CDS             373..1314
                     /locus_tag="VN627_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXD02643.1"
                     /translation="MLDPSAARIDRDFARTIDWNLLKVFLEIVRAGGIGAAARTLGKQ
                     QPSISASLKRLEDQLGARLFDRSTAGIRITPAGKALQALCEDLFETVRMAQHQVAQAT
                     KQVGGAVRIQLVSSIVCTEFDEAIASFHRRHPAITMELRVSPWRGVLEALASGEVEVG
                     IGYEGAAGPELVYEPMFIETQQLYCARAHPLYGRKLERLADLRREGFVLTGADEPEAV
                     TRLRSRHGLGEEQAGLAEDVHEALRLVSMGVGIGFLPEGAAESEVRAGRLWPLLPDDQ
                     QVAYEIFLISRADPSRDTATQLFLDEIRRRLRARRPS"
     gene            1407..3245
                     /locus_tag="VN627_04975"
     CDS             1407..3245
                     /locus_tag="VN627_04975"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HXD02644.1"
                     /translation="MTILAGLWQRLLLSRPGQPVDTLTRVDWLQGPVLYADLRQPPEL
                     TRRIAASCLAELSVDEARLLATQEGFAGRFALAGNLAEWHREIDFRPCPLGDRGRLED
                     HGDLLVEHGVDADYIEHWQREPAPRGVPSGSARFVSEADGRAVILVRHGPRFAYARAR
                     GMALAPGELERRLGSASAAEARGLIDCEISFGTVTPQAWRIDRSSLPWRVGDLLLSIG
                     ARIAGARLDVADTAADGLPFARRLRLVELHGSLLPDDSGLQPEAPALPPAPGESTGPF
                     QSIPVIDMAALGGGDPAAEAACVAAIREAAAHVGFLMVTGHGIAPEVAARLQAAARRF
                     FEQPLAAKQACYIGQSTNHRGYVPEGEEQLGDPGLDTTNKVPDRKEAFDLALDLAADC
                     VPAGHLMLGPNQWPALPGFREDVMAYYGAVFAVGRRLLRGFALALGQRGDAFDHLVTM
                     PPSQLRLIHYPHDAAAADAPGIGAHTDYELFTLLLATGPGLEVMNQAGEWIDAPPVPG
                     ALVVNIGDMLEYWSAGAFVATSHRVRKVGAERWSFPLFFALDYDVELRPLGQREAAPI
                     RTGEHLYAQTAQTFRYLKDRALPEAARPVSSFGQVARHRHSIPDAA"
     gene            3271..>3537
                     /locus_tag="VN627_04980"
     CDS             3271..>3537
                     /locus_tag="VN627_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase"
                     /protein_id="HXD02645.1"
                     /translation="MKLTFRKTLQAAALASLVLTAACSGAKKEAAAPAAEAPKKEFNI
                     GWSIYAGWMPWPYAEKSGILKKWADKYGIKINLIQVNDYVESVNQ"
BASE COUNT          547 a         1271 c         1170 g          549 t
ORIGIN      
        1 gttcgaatgc gcgggcgcct atggctcgct cgaccaggcc atctccgccg tgcggcgcgg
       61 cggcacggtg gccgcgcccg ggttccactg ggggcaggac aagttcttcg gcatgggcgc
      121 gctgatcaag gaagtgacca tccgctacac caacatgtac aacaccgccg agttcgcccg
      181 cgccgtcgac acgctcgacc tcgggcatgt cgaaccccgc gcgatgatca ccagcacggt
      241 cgaccttgcc ggcgccccgg ccgccttcga ggaactgcgc ggcgccaaca cgcaatgcaa
      301 ggtgatgatc cgcccctggt gaccagcgcg gggcggctcc acaaaccggc tccaccccgc
      361 taggctgcgg ccatgctcga cccttccgcc gcacggatcg accgcgactt cgcccggacc
      421 atcgactgga acctgctcaa ggtgttcctc gagatcgtcc gcgccggcgg catcggtgcg
      481 gcggcgcgga cgctcggcaa gcagcagccc agcatcagcg cctcgctgaa acggctggaa
      541 gaccagcttg gcgcccgcct gttcgatcga tccaccgccg gcatccgcat cacccccgcg
      601 ggcaaggccc tccaggcctt gtgcgaagac ctgttcgaga ccgtgcgcat ggcccagcac
      661 caggtcgccc aggcgaccaa gcaggttggc ggcgcggtgc ggatccagct ggtcagctcg
      721 attgtctgta ccgagttcga cgaggcgatc gcctcgttcc accgccgcca cccggcgatc
      781 accatggaac tgcgcgtgtc gccttggcgc ggtgttctcg aagcgctcgc ctcgggcgaa
      841 gtggaagtcg gtatcggcta cgaaggagcg gcgggcccgg aactggtcta cgagccgatg
      901 ttcatcgaaa cccagcagct ctactgcgcc cgggcccacc cgctctacgg ccgcaagctg
      961 gagcggctgg ccgaccttcg gcgcgaaggc ttcgtactga cgggggccga cgagccggag
     1021 gcggtcaccc ggctgcgcag ccggcacggc ctgggcgagg aacaggccgg ccttgccgaa
     1081 gacgtgcacg aggcactgcg gctggtgtcg atgggtgtcg gcatcggctt cctgcccgaa
     1141 ggcgccgcgg aaagcgaagt tcgggccggc cggctatggc cgctgctgcc ggacgaccag
     1201 caggtcgcct acgaaatctt cctcatctcg cgcgccgatc cctcgcgcga cacggccacc
     1261 cagctgttcc tcgacgagat ccgccggcga ctgcgcgcac gccgaccctc atagccttaa
     1321 cctatgagcc tcgtcattat ttgatagcta gcggtatgag ccgaatcgcg tagcgtttcg
     1381 cccatgatcc tggtgcagac atttgcatga ccatcctcgc cggcctgtgg cagcgcctgc
     1441 tgctctcgcg gcccggccag ccggtggata ccctcacccg ggtggattgg ctgcaggggc
     1501 ccgtgcttta cgccgacctg cggcaaccgc ctgagttgac ccgacggatc gccgcctcgt
     1561 gcctcgccga actcagtgtt gacgaggccc ggctgctggc gacacaggaa ggcttcgccg
     1621 gacgcttcgc gcttgccggc aaccttgccg agtggcaccg cgagatcgat tttcgcccct
     1681 gcccgctggg tgatcgcggc cgcctggaag accatggcga cctccttgtc gagcacggcg
     1741 tcgatgccga ctatatcgaa cactggcagc gcgaaccggc gccgcgcggg gtgccgagtg
     1801 gctcggcccg gttcgtgtcc gaagcagacg ggcgggcagt gatcctcgtc cgccatggac
     1861 cgcgctttgc ctatgcccgc gcccgcggca tggctcttgc cccgggcgaa ctggagcgcc
     1921 gcctcggctc ggccagcgcg gccgaagcgc gcggccttat cgactgcgag atttctttcg
     1981 gcacggtaac gccgcaagcc tggcggatcg accggtcgag cctgccctgg cgcgtgggcg
     2041 acctgctgct gagcattggc gcgcggatcg ccggggcgcg gctcgacgtg gccgatactg
     2101 ccgccgatgg cctgcccttc gcccgccggt tgcggctggt ggaactgcat ggctcgcttc
     2161 tgccggatga cagtgggctg cagccggaag ccccggccct cccgcccgca cccggcgaaa
     2221 gcacgggccc attccagtcg atcccggtca tcgatatggc cgcgctcggc ggcggcgatc
     2281 ccgccgcgga ggcggcctgt gtcgcggcga tccgcgaagc ggcggcccac gtcggcttcc
     2341 tcatggttac cggccacggc atcgcgccgg aagtggcggc gcggctgcag gcagcggcgc
     2401 ggcggttttt cgaacagccg ctggccgcca agcaggcctg ctacatcggc cagtcgacca
     2461 atcaccgcgg ctatgtcccc gagggcgaag aacagctcgg cgatccgggc ctcgatacaa
     2521 cgaacaaggt ccccgatcgc aaggaggcct tcgacctcgc cctcgatctc gccgccgatt
     2581 gcgtcccggc gggccatctg atgctcgggc cgaaccagtg gccggcgctc cccggcttcc
     2641 gcgaggacgt catggcctac tatggcgcgg tcttcgccgt gggccggcgg ctgctgcgcg
     2701 gctttgccct tgccctgggg cagcgcggcg atgccttcga ccacctcgtg accatgccgc
     2761 ccagccagct gcggctgatc cactatcccc acgatgccgc agccgccgac gcacccggca
     2821 tcggcgcgca taccgactac gagctgttca cgctgctgct ggccacgggg ccgggcctgg
     2881 aggtcatgaa ccaggcgggg gaatggatcg atgccccgcc cgtgcccggc gcgctggtgg
     2941 tgaacatcgg cgacatgctg gaatactggt cggcaggggc cttcgtcgcc acctcgcacc
     3001 gggtccgcaa ggtcggcgcc gaacgctggt cgttcccgct gttcttcgca ctcgactacg
     3061 atgtggaact gcggccgctc ggtcagcgcg aagccgcgcc catccgcacc ggcgagcatc
     3121 tctacgccca gacggcgcag accttccgct atctcaagga ccgcgccctg cccgaggctg
     3181 cgcgcccggt ctcctcgttc gggcaggtcg cccgccaccg ccattccatt ccagacgccg
     3241 catgaagcgg cggagcccag agggggacgc atgaaactga ctttccgcaa gaccttgcag
     3301 gcagcggcgc ttgccagcct ggtgctgacc gcggcctgct ccggcgccaa gaaggaggcc
     3361 gccgccccgg ccgccgaggc gcccaagaag gagttcaaca tcggctggtc gatctatgcc
     3421 ggctggatgc cctggcccta tgccgagaag agcggcatcc tcaagaagtg ggccgacaag
     3481 tacgggatca agatcaacct gatccaggtg aacgactacg tcgagagcgt gaaccag
//
LOCUS       DATNBV010000204         2763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1703794,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000204 DATNBV010000000
VERSION     DATNBV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703794"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VN627_04985"
     CDS             <1..515
                     /locus_tag="VN627_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXD02646.1"
                     /translation="GALAAPAEEIAAVRDALRGEQAQVRGLIDTLRDPSGLLAHRPLA
                     ADLDRALTEAAAHWHVAASFAIDAPIEAAAGLSHELRQLLREAVANAARHGNASRIDA
                     RLGRTGGGLELVVEDDGSGFDEASGKTPPWSIGERVAALGGRLDVVTGSGGTRLSIFL
                     PLSSGPEQTS"
     gene            512..1144
                     /locus_tag="VN627_04990"
     CDS             512..1144
                     /locus_tag="VN627_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXD02647.1"
                     /translation="MTTVLIADDHPFIRAGVQAVLEPTAFRIVAAAGSGAEALAALKE
                     HDPEVCIFDVTMPAPDGVETLKAMREAGDRRPVILLTAHIDDQRLAEAVAAGVNGILS
                     KDGAEEVLAGSLERVMAGETVIAPDLLARARLQAERRSRPSPFAVLTPRERTIVALAM
                     RGTRNREIAAELGITEGTVKVYLHALYQKLGVDNRTELAVLALKHPDEFA"
     gene            1389..>2763
                     /gene="zwf"
                     /locus_tag="VN627_04995"
     CDS             1389..>2763
                     /gene="zwf"
                     /locus_tag="VN627_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXD02648.1"
                     /translation="MSFTADCLLLFGATGDLAQRMLLPSLCALDADRLVAPDLRIVGT
                     ARQGLDDEGYRAFARTALEKFLPEDRKAAIDPFLERLHYQPLDANETAHFDSLAAKVG
                     PIGGGLAIFLSTAPSLFGPTIAGLVGSGLAGEKTRIGLEKPLGTDLASSRVINDAVAA
                     AFPENRIFRIDHYLGKETVQNLLALRFANLLFEPLWNAAHIDHVQITVAETVGLESRV
                     GYYDGSGALRDMVQNHMLQLLALVAMEPPASFDSTAVRDEKVKVLRSLRKVACDQTVT
                     GQYRAGAIGGQAVPGYDQELGKPSDTETFVALKAHVDNWRWKGVPFYLRTGKRLPERV
                     TEIVVQFRNVPHSIFAARGAATMPNRLIIGIQPEENITLSLMAKVPGLDRGGIRLREV
                     PLDIAMDDAFAGPKKRIAYERLLLDLVEGDQTLFVRRDEVEAQWEWVDAIRTCWVDNG
                     ITPKTYTA"
BASE COUNT          443 a         1041 c          856 g          423 t
ORIGIN      
        1 atggtgccct cgccgcgccg gccgaggaaa tcgccgccgt gcgcgatgcc ctgcgcggcg
       61 aacaggcgca ggtgcgcggc ctgatcgata ccctgcgtga tccctccggt ctgcttgccc
      121 atcgcccgct tgccgccgac ctcgaccgcg ccctgaccga agcggcggcc cactggcacg
      181 tcgctgccag ctttgccatc gatgcgccga tcgaagctgc cgccgggctc agccacgaat
      241 tgcgccagct cctgcgcgaa gccgtggcca atgccgcccg ccacggtaac gcctcccgca
      301 tcgacgctcg cctcgggcga accgggggcg gcctcgaact ggtcgtcgag gacgatggca
      361 gcggcttcga cgaagcctcc ggcaagaccc cgccgtggtc catcggcgag cgcgtggcgg
      421 cactcggcgg caggctcgac gttgtaaccg gcagcggcgg aacccgtctc tccatcttcc
      481 ttccgctctc cagcggcccg gagcaaactt catgaccacc gtgctgatcg ccgacgacca
      541 tcccttcatc cgcgccggtg tccaggccgt tctggagccg accgcgttcc gcatcgtcgc
      601 tgccgcgggg tccggggccg aggcgctcgc cgcgctcaag gaacacgatc ccgaagtctg
      661 catcttcgac gtgaccatgc ccgcgcccga tggcgtggaa acgctcaagg cgatgcgcga
      721 ggcgggtgac cgtcgcccgg tcatcctcct caccgcccat atcgacgacc agcgcctcgc
      781 cgaagccgtg gcagccgggg tcaacggcat cctcagcaag gacggggccg aggaggtcct
      841 ggccgggtcg ctcgaacggg tcatggccgg cgagacggtc atcgcgcccg atctgcttgc
      901 ccgcgcccgc ctgcaggccg aacggcgcag ccgcccttcg cccttcgccg tgctcacccc
      961 gcgcgagcgc accatcgtgg ccctagccat gcgcggcacc cgcaaccgcg agatcgccgc
     1021 cgaactcggc atcaccgagg ggacggtcaa ggtctacctc cacgccctct accagaagct
     1081 cggggtggac aaccgcactg aactggccgt cctcgccctg aagcaccccg acgagttcgc
     1141 ctgattcccg gcccatgccg gcccgggctg cggcgcagac gaatgcaccg gcatgtgcag
     1201 acgtattctt ggcgacgccg cgctccccct ggataaggac cggtcctgac cggatgcggt
     1261 tgcccctgat tggcgcttgc ctgcgcggcg ccatggagga acactcgccg ccagatcggg
     1321 ccgcctgatc cggccgaata tcgggaacgc ctgccgatgc cgtccgtagc caacggggat
     1381 ttgcgacaat gagcttcacc gccgattgcc tgctgctgtt cggcgccacg ggtgacctgg
     1441 cgcagcgcat gctgctgccc tcgctctgcg cgctcgatgc ggaccggctg gtcgcgcccg
     1501 acctgcgcat cgtcggcacg gcccgccagg ggctagacga tgaaggctat cgcgccttcg
     1561 cccgcaccgc gctcgagaaa ttcctcccgg aagaccgcaa ggccgcgatc gaccccttcc
     1621 tcgaacgcct ccactaccag ccgctcgatg cgaacgagac cgcccatttc gattcgctgg
     1681 cggccaaggt cggtccgatc ggcggaggcc tcgccatctt cctgtcgacc gcgcccagcc
     1741 tgttcgggcc gaccatcgcc ggactcgtcg gctcgggcct tgccggcgag aagacccgca
     1801 tcggcctcga aaagccgctc ggcaccgatc tcgcctcgag ccgggtcatc aacgacgctg
     1861 tcgccgccgc cttcccggaa aaccgcatct tccggattga ccactacctg ggcaaggaaa
     1921 ccgtccagaa cctgctggcc ctgcgcttcg ccaatctcct gttcgagccg ctgtggaacg
     1981 ccgcgcatat cgaccatgtc cagatcacgg tggcggaaac cgtgggtctt gaatcccgcg
     2041 tcggctacta cgacggctcg ggcgccctgc gcgacatggt ccagaaccac atgctccagc
     2101 tgctggcgct cgtcgccatg gagccgccgg caagtttcga ctccaccgcc gtgcgcgacg
     2161 aaaaggtcaa ggtcctgcgc tcgctgcgca aggtcgcctg cgaccagacc gtcaccggcc
     2221 agtaccgcgc cggagccatc ggcggccagg ccgtgcccgg ctatgaccag gaactgggca
     2281 agcccagcga caccgagacc ttcgtcgcgc tcaaggccca tgtcgacaac tggcgctgga
     2341 agggtgtccc cttctacctg cggaccggca agcgcctgcc cgagcgggtg accgagatcg
     2401 tcgtccagtt ccgcaatgtg cctcattcga tcttcgccgc gcgcggggcg gcaaccatgc
     2461 ccaaccgcct gatcatcggc atccagcccg aagagaacat caccctttcc ctcatggcca
     2521 aggtgccggg gctcgaccgc ggcggcatcc gcctgcgcga agtccccctc gacatcgcca
     2581 tggacgacgc cttcgccggc ccgaagaagc gaattgccta cgagcgcctc ctgctcgatc
     2641 tggtcgaggg cgaccagacc ctgttcgtcc ggcgtgacga ggtggaggcg cagtgggaat
     2701 gggtcgatgc gatccgcacc tgctgggtcg ataatggcat cacccccaag acctacaccg
     2761 ccg
//
LOCUS       DATNBV010000205         9562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_429993,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000205 DATNBV010000000
VERSION     DATNBV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429993"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2913
                     /locus_tag="VN627_05000"
     CDS             <1..2913
                     /locus_tag="VN627_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXD02649.1"
                     /translation="QAQVPGVGGTWKDLTDNVNLMATNLTNQVRGIADVVTAVAQGNL
                     KRKLTFDAKGEIAALAETINGMIETLSTFGDQVTNMAREVGVEGRLGGQARVPGAAGL
                     WRDLTDNVNAMAANLTNQVRSIAEVATAVTKGDLTRSIAVEASGEMAALKDNINEMIL
                     NLKDQTLKNAEQDWLKTNLARFSRMLQGERDLATISNLIMSELAPLVNAQYGVFYVTA
                     REEEDTRLDLVASYGAEDHQQLKRQFGLREGLIGQAAADKRPIRLDKVPGDFIRIGSG
                     LGQGSPANIQILPALFEDEVKAVIELASFEEFSETHHSFLTQLMETVGIVLNTIAATM
                     RTEGLLAQSQLLTQELQARQTELTRKQEELHATNEELQEKAQLLENEKRQVEAKNFEI
                     DMARRAVEEKAEQLALTSKYKSEFLANMSHELRTPLNSLLILSKLLADNQQGNLNEKQ
                     TEFARTIHSAGTDLLNLINDILDLSKIESGTVSIDIGDMPLAAMKQHLERTFRQLASE
                     KDLAFQVEFDEALPQSIQTDEKRLQQIVLNLLSNAFKFTARGGVTLRARPASRGWSPT
                     HPVLGELETALEISVQDTGIGIPEDKQKLIFEAFQQADGTTSRKYGGTGLGLSISREI
                     ARLLGGELQVRSVPGEGSTFTLYVPMKAVSAPNALLPAPATDGEPMMGFAPQDDRDDL
                     CGEPFVLIVEDDPTFATILLELAHQSGLKGVISSAGSGTVALVRKLRPSAITLDLGLS
                     DIDGFVLLDLLRHEPDTCDIPVYVISGAEQAAHALTLGASGITEKPANRDELCDLFNS
                     IVETARAATPRKPPRRAAKGRPAEQQGLAGSRILIVDDDIRNIYSLTSVLESHDVEVI
                     HAERGAEGIRLLETTPEIDAALIDIMMPEMDGYETMRQIRLTPAIAHVPLVAVTAKAM
                     KGDRQKCLDAGASDYISKPVDIDLLLALLRVWIGRSRSQGAAGRTTQPARRPVRVA"
     gene            2913..4967
                     /locus_tag="VN627_05005"
     CDS             2913..4967
                     /locus_tag="VN627_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXD02650.1"
                     /translation="MPPLAKPAAAPDVPPDLGPLDHGEDFASTARILLVDDDERNLLA
                     LGHVLRDVGEVVTASSGREALRQLLHGEFAVILLDVFMPEMDGYEVAELIRERRQTAR
                     IPIIFLSAVNKETEHLMRGYAMGAVDYVFKPVDPVVLTSKVGVFVDLFDMRKRVEAKN
                     RAERELREASFRAQLERLQIENELSASRARQASVLEALPLALFEAVSDGAGMLTREFV
                     AGDIARLAGADAAALESGMLCWEERIHPDDLSALGMPADPQDAFSAEYRWRCADGSHK
                     HFIERAVPISCDAGGTTRWAGTLFDVTDRKQLEAQLVQAGKMDALGQLTGGVAHDFNN
                     VLAAVLGGARLLERRAPLDERHRRIVEQMRVAAERGADLVRRMMAFARRQELTPVYLT
                     PSAVCDEVAGLVEQTLGSQVTLEWDCSACDLVFHADRSQLELALVNLIINARDAMPEG
                     GTVRVAISELACAPSGQIGAASSLLIEVTDTGSGMPPEVLERITEPFFTTKGVGKGTG
                     LGLSMVAGFVQQSGGSIDIVSEVGKGTAVRITMPAVRPRPAEPGAPHEPAAGEGMVLR
                     HVIVVDDDPAVRSIVAEQLRELGASVREMASGADALAALAAASEPVELLLTDYAMPGL
                     NGLQTIAEARVLRPELGCALMTGYADDRIEALIPHSTRLLRKPIAPGDLVTLLSAPVA
                     TA"
     gene            4993..5370
                     /locus_tag="VN627_05010"
     CDS             4993..5370
                     /locus_tag="VN627_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02651.1"
                     /translation="MKQLRSEQVLAFITTTFRSAWAIELLCLLRSDPQRSYSPGELVR
                     LLSASRVVVEQSVAALAALGLVSTEDDGTVRFDAPDHMDGLVAAVAELYQRSPDAVRR
                     AIARGNSRSLAAFADAFRLRSDE"
     gene            5367..5639
                     /locus_tag="VN627_05015"
     CDS             5367..5639
                     /locus_tag="VN627_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HXD02652.1"
                     /translation="MTEVLPAAVYLLCFLTSTACAVLLARSYARTRLRLLLWSAICFG
                     LLAGNNLTLVVDLLLLPQVDLRLLRHLFALAAIAVLLFGFVWERDD"
     gene            5639..5905
                     /locus_tag="VN627_05020"
     CDS             5639..5905
                     /locus_tag="VN627_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HXD02653.1"
                     /translation="MESLLKFTAGAITMGFLVAALFFLRFWRTTREGLFLSFAFAFFL
                     LGAGQAMLTFTDMGDEDRSWLFVVRLAAFSCILLAIWRKNRASR"
     gene            6069..7121
                     /locus_tag="VN627_05025"
     CDS             6069..7121
                     /locus_tag="VN627_05025"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXD02654.1"
                     /translation="MSLFESLGVSLPIVQAPMVGVSTPELAAAVCNAGALGSIGVAAT
                     DARGAGEMIAAVRTLSSRALNVNVFVHQPARRDTAKEAAWLAAMEPLFRQYGVQPPPE
                     LRPIYKSFLEDDEMLALLVETAPAVVSFHFGLPDAGRIAALKNAGCTLFATATNLEEA
                     VAAEAAGIDAVVAQGWEAGGHRGVFDPASPDSRLGTLPLTRLLVQRCGLPIIAAGGIM
                     DGAGIRAALDLGAMAAQLGTAFVACPESSADAAYREALAGPAAMDTVMTSAISGRPAR
                     GLANRFTAWGESVGSPHPGYPMTYDAGKALIAAAKAAGETGYAAQWAGQGAPLSRAMP
                     AAELVAVLAKELHFES"
     gene            complement(7136..7927)
                     /locus_tag="VN627_05030"
     CDS             complement(7136..7927)
                     /locus_tag="VN627_05030"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXD02655.1"
                     /translation="MLDEDHRKLLGAFVRARREAIVPDTPGRRRRTPGLRREELAARA
                     GIGATWVAWIEQGRAVRASAETMGRLADSLDLTQAERTYLFSLAGHRDPADPFAQAPG
                     AAPLAITALVSRLDWPAYGLDAAWTVCCANAPARRLFVGLFDGEAQPNLLRYVFTSPE
                     ARRLLPDWAERARRLLAEFRRDYGRGLSDPRVAGVVDWLLENSEHFRNGWEEQAVMSR
                     EGGVRVFQHPDEGELRFIQHTLGDVTRGDFRLVALEPAVKSAGSP"
     gene            8009..8764
                     /locus_tag="VN627_05035"
     CDS             8009..8764
                     /locus_tag="VN627_05035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXD02656.1"
                     /translation="MTPHNSLVGKQFGVAAADYVTSTVHAEGPDLRRIAEVAAAHAPR
                     RALDLGCGGGHVAYALAPHSGEVIASDLSAEMTAAVAAEAARRGLGNIHTVTAPAEAL
                     GFPDQSFDLLACRFTVHHWRDAAAGLREARRVLRRGAPALFADVIAPDLAAADTHLQA
                     AELLRDPSHGRDYRLGEWRTMLEQSGFRITAITTGQLRMDFADWTARMRTTPDRVAAI
                     RALQTLASDEVAHHFAIEADGSFTIDTALIEAC"
     gene            complement(8811..>9562)
                     /locus_tag="VN627_05040"
     CDS             complement(8811..>9562)
                     /locus_tag="VN627_05040"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD02657.1"
                     /translation="MGASTVRLLASLGADVAVLDVDRERAESTAAEAAKEGVRSAAIV
                     GDVFDDGAVGRAVDEAEEAIGPLSGMAAIVGMAGWSSLLDMTPQTWDMDQQRNLRYFF
                     MIAQAVARKLVSRGMPGSIVGVCSVDGIRSAPHHASYGAAKAGLMNLVRSMTMEWAEH
                     GIRVNLVAPGAIITPRIPEREAGLERQMSACIPMGRRGTTDEIAKAITFFLSDMSSYV
                     SGQTLAVDGGYLSAGLFGTPAVDKPGGTLGA"
BASE COUNT         1654 a         3274 c         3057 g         1577 t
ORIGIN      
        1 caggcgcagg tgccgggcgt gggcggcacc tggaaggacc tcaccgacaa cgtcaacctc
       61 atggcgacca acctcaccaa ccaggtgcga ggcatcgcgg acgtcgtcac cgcggtggcc
      121 cagggcaacc tcaagcgcaa gctcaccttc gatgccaagg gcgagatcgc ggcactggcc
      181 gagacgatca acgggatgat cgagacgctc tccaccttcg gcgaccaggt caccaacatg
      241 gcccgcgagg tcggcgtgga aggccggctc ggcgggcagg cgcgcgtgcc gggcgcggcg
      301 ggcctgtggc gcgacctgac cgacaacgtc aacgccatgg ccgccaacct caccaaccag
      361 gtgcggtcga tcgccgaagt ggcgaccgcc gtgaccaagg gcgaccttac ccgctcgatc
      421 gcggtggaag cctcgggcga gatggccgcg ctcaaggaca acatcaacga gatgatcctc
      481 aacctgaagg atcagaccct caagaacgcc gagcaggact ggctcaagac caacctcgcc
      541 cggttcagcc gcatgctcca gggcgagcgc gaccttgcca ccatttccaa cctgatcatg
      601 tcggaactgg ccccgctggt gaacgcgcag tacggcgtgt tctatgtgac ggcccgcgag
      661 gaggaggaca cccggctcga cctcgtcgcc agctacggcg cggaggacca ccagcagctc
      721 aagcgccagt tcggtctgcg cgaggggctg atcggccagg cggcggcgga caagcggccg
      781 atccggctgg acaaggtgcc gggcgacttc atccgcatcg gctcgggcct tggccagggg
      841 tcgccggcca atatccagat cctgccggcc ctgttcgagg acgaggtcaa ggcggtcatc
      901 gagctcgcct cgttcgagga gttcagcgag acccaccact cgttcctaac ccagctgatg
      961 gaaacggtcg gcatcgtgct caacacgatc gccgcaacca tgcgcaccga gggtctgctg
     1021 gcccagtcgc agctgctcac gcaggagctc caggcccgcc agaccgagct cacccgcaag
     1081 caggaggaac tgcacgccac caacgaggaa ctgcaggaaa aggcccagct gctcgagaac
     1141 gagaagcgcc aggtcgaggc caagaacttc gagatcgaca tggcccgccg ggcggtggag
     1201 gaaaaggccg agcagctcgc cctcacctcc aagtacaagt ccgaattcct cgccaacatg
     1261 agccacgagc tgcgcacgcc gctcaactcg ctgctgatcc tgtccaagct tctcgccgac
     1321 aaccaacagg gcaacctcaa cgagaagcag accgagttcg cccgcaccat tcacagcgcc
     1381 ggtaccgacc tgctcaacct catcaacgac atcctcgacc tgtcaaagat cgaatccggc
     1441 acggtctcga tcgacatcgg cgacatgccg ctggcagcga tgaagcagca tctcgagcgc
     1501 accttccgcc agctcgcctc ggagaaggac ctcgccttcc aggtcgaatt cgacgaggcc
     1561 ctgccccagt cgatccagac cgacgagaag cgcctgcagc agatcgtgct caacctgctg
     1621 tctaacgctt tcaagttcac cgcgcgcggc ggtgtcaccc tgcgtgcccg gcccgcgtcg
     1681 cgcgggtgga gcccgacgca ccccgtgctc ggcgaactgg agacggcgct ggaaatctcg
     1741 gtccaggata ccggcatcgg tatcccggaa gacaagcaga agctgatctt cgaggcattc
     1801 cagcaggcag acggaaccac cagccgcaag tacggcggca cgggcctggg cctgtcgatc
     1861 agccgcgaga tcgcccgtct gctgggcgga gagctccagg tgcgatcggt cccgggtgag
     1921 ggttcgacct tcaccctcta tgtgccgatg aaggcggtct ccgcgccgaa cgcgctgctg
     1981 ccggcgcccg ccaccgatgg cgaacccatg atgggctttg cccctcagga cgaccgcgac
     2041 gatctttgcg gcgaaccatt cgtgctgatc gtcgaggacg atccgacctt cgcgacaatc
     2101 ctgctcgaac tcgcccacca gtccggtctc aaaggtgtca tttcctcggc cggctcgggc
     2161 acggtagccc tcgtccgcaa gctgcggcca agcgccatca ccctggacct cgggctctcg
     2221 gacatcgacg gcttcgtgct gctcgatctt ctgcgccacg agcccgatac ctgcgacatc
     2281 ccggtctacg tcatctccgg cgcggagcag gcggcccatg cgctgacgct cggcgcttcc
     2341 ggcatcaccg aaaagccggc gaaccgcgac gaactgtgcg acctgttcaa ttcgatcgtc
     2401 gagaccgcac gagcggctac gccgcgcaag ccgccccggc gcgcagccaa gggccggccg
     2461 gcagagcagc aggggctggc cgggagccgc atcctcattg tcgatgacga tatccgcaac
     2521 atttactcgc tcaccagcgt gcttgaatcc cacgatgtcg aggtaatcca cgccgaacgc
     2581 ggcgcggagg ggatccgcct gctcgagacc acgcccgaga tagatgccgc gctgatcgac
     2641 atcatgatgc ccgaaatgga tggttacgag accatgcgcc agatccgcct gaccccggcc
     2701 atcgcccatg tcccgctggt cgcggtcacg gccaaggcga tgaagggcga ccggcagaag
     2761 tgcctcgacg ccggcgcctc ggactatatt tccaagccgg tggacatcga cctgttgctg
     2821 gccctgctgc gggtgtggat cggtcgtagc cggtcgcaag gcgccgccgg gcggaccacg
     2881 caaccggcgc ggcggcccgt gcgggtcgcc tgatgccccc gctcgccaag cccgcggccg
     2941 ctccggacgt accgcccgac ctcggcccgc ttgaccacgg ggaggacttc gccagcacgg
     3001 cccgcatcct gctggtggac gacgacgagc gcaatctcct cgcgctcggc cacgtgctgc
     3061 gcgatgttgg cgaagtggtc accgcgagtt ccgggcgcga ggccctgcgg cagctcctcc
     3121 acggggagtt cgccgtcatc ctgctcgacg tcttcatgcc ggagatggac ggctacgagg
     3181 tggccgagct gatcagggag cgtcggcaga ccgcacgcat tccgatcatc ttcctctcgg
     3241 cggtgaacaa ggaaaccgag cacctgatgc gcggctatgc catgggagcg gtcgattacg
     3301 tgttcaagcc ggtcgacccg gtggtgctga cctcgaaagt cggcgtgttc gtcgacctgt
     3361 tcgacatgcg caagcgcgtc gaggccaaga accgagcgga gcgggaactg cgcgaagcca
     3421 gcttccgggc gcagctcgaa cgcctgcaga tcgaaaacga actgagcgcc tcgcgcgcgc
     3481 gccaggcgag tgtgctcgag gccctgccct tggcgctgtt cgaggccgtt tcggacggtg
     3541 cgggcatgct gacgcgcgaa ttcgttgccg gtgacattgc ccggctcgcg ggcgctgatg
     3601 ctgcggcgct ggaaagcggc atgctatgct gggaagagcg cattcacccc gacgatctct
     3661 ctgcgctcgg catgccggcc gacccgcagg acgccttctc cgcagagtat cggtggcgct
     3721 gtgccgacgg ctcgcacaag catttcatcg agcgcgccgt tccgatcagc tgcgacgccg
     3781 gcggcaccac ccgctgggcc ggcaccctgt tcgacgtaac cgaccgcaag cagcttgaag
     3841 cgcagctcgt ccaggccggc aagatggatg cgctcggcca gctgaccggc ggcgtggcgc
     3901 atgatttcaa caatgtgctc gccgcggtgc ttggcggagc gcgactgctg gaacgccggg
     3961 cgcccttgga cgagcgccac cgccggatag tcgagcaaat gcgggttgcc gccgagcgcg
     4021 gagccgatct ggtccggcga atgatggcct tcgcccgcag gcaggaactg accccggtct
     4081 acctcactcc ctcggcggtg tgcgacgaag tcgccgggct ggtcgaacag acgctgggga
     4141 gtcaggtcac gctcgaatgg gactgttcag cctgcgacct ggtgttccat gccgaccgct
     4201 ctcaacttga actcgcgctg gtcaacctga tcatcaacgc gcgcgacgcc atgccggaag
     4261 gcgggaccgt ccgcgtggcg atatcggaac ttgcctgcgc gccgtcaggc cagatcggtg
     4321 ccgcttcatc gctcctgatc gaagtaacgg acaccggttc gggcatgccg cccgaggtgc
     4381 tggagcggat taccgagcca ttcttcacga caaagggcgt cggcaagggc acggggcttg
     4441 gcctatccat ggttgccggc ttcgtgcagc agtcgggcgg cagcatcgac atcgtcagcg
     4501 aggtcggcaa gggtacggca gtgcggatca ccatgcccgc cgtgcggccc cggccagccg
     4561 aacccggagc tccacacgag ccggctgccg gcgagggcat ggtgttgcga catgtgatcg
     4621 tcgttgacga cgatccggcg gtgcgctcga tcgtggcgga acagctgcgc gagctcggcg
     4681 ccagcgtccg cgagatggcc agcggtgcgg atgccttggc ggcccttgct gcggcaagcg
     4741 aaccggttga actgctcctc accgactatg ccatgcccgg cctcaacggc ctccagacga
     4801 tcgccgaggc gcgagtgctg cgtcccgaac tgggctgtgc gctgatgacc ggctatgcgg
     4861 acgatcgcat cgaagccttg atcccgcatt cgaccaggct actgcgcaag ccgatcgctc
     4921 ccggcgatct ggtcaccctg ctcagtgccc cggtggcgac ggcctgagcc ggcacgacag
     4981 acgcaaccgg ccgtgaagca gctgcgcagc gagcaggtcc tcgcctttat caccacgacc
     5041 ttccgctcgg cctgggcaat cgagttgctg tgcctgctgc gctcggatcc tcagcgcagc
     5101 tattcgcccg gtgaactggt gcgcctgctc tcggccagcc gggtggtggt cgagcagtcg
     5161 gtggccgccc ttgcggcctt gggcctggtg tcgacagaag acgacggaac cgtgcgcttc
     5221 gacgcaccgg atcacatgga cggcctggtg gcggcggtcg ccgagcttta ccagcgttcg
     5281 cccgatgcgg tgcgccgcgc cattgcccgc ggcaattcgc ggagccttgc cgcctttgcc
     5341 gatgccttcc ggctgaggag cgacgaatga cagaagtcct gcctgcagcc gtctacctct
     5401 tgtgcttcct gacgagcacg gcctgcgcgg ttctgcttgc ccgctcctat gcccgcactc
     5461 gcctgcgtct tctcttgtgg agcgccatct gcttcggatt gctggcgggg aacaacctca
     5521 ccctggtggt cgatctgctg ctcttgccgc aagttgatct gcgcctcctg cgccacctgt
     5581 tcgccctcgc cgcgatcgcg gttctgctgt tcgggtttgt ctgggagcgc gacgactgat
     5641 ggaaagcctg ctcaagttta ccgccggtgc catcaccatg ggcttcctcg tggccgcgct
     5701 gttcttcctg cggttctggc ggacaacgcg cgaaggtctg tttctgtcat tcgcctttgc
     5761 cttcttcctc ctcggtgccg gacaggcaat gctcacgttc acagacatgg gcgacgaaga
     5821 ccgctcgtgg ctgttcgtcg tccgcctcgc agcgttttca tgcatccttc tcgcgatctg
     5881 gcggaagaac cgggccagcc ggtgaggaca cgggagatcc tgcgcctacc ggtgcgcgct
     5941 ccggagcaga ccgtccagat tccgcccctg caacagccgg cctgaacggc tgcggtgttg
     6001 cgcggagcgg agatacccgc gcgtaaaacg ctatcctgat tacaggacgg ccgtgataag
     6061 gcggggccat gtccctattt gaaagtctcg gggtatctct gccgatcgtg caggcgccaa
     6121 tggtcggggt gtccactccg gaacttgccg cggccgtctg caacgccggt gcgctcggat
     6181 ccatcggggt tgccgccacc gacgctcgcg gcgcggggga gatgatcgct gctgtccgca
     6241 ccctcagcag ccgcgcgttg aacgtcaacg tgttcgtcca tcagccggcg cggcgggata
     6301 ccgcgaagga ggcggcgtgg ctggcggcga tggaaccttt attccgccaa tatggcgtgc
     6361 aaccgccacc agaactgcga cccatctaca agagtttcct ggaagacgac gagatgctgg
     6421 cgctgctggt ggaaacggcc cctgcagtgg tcagcttcca tttcggcttg cccgatgccg
     6481 gacggattgc cgcgctcaag aatgccggct gtaccttgtt cgccacggcg actaacctcg
     6541 aagaagcggt tgcggccgaa gcggctggaa ttgacgcggt ggtagcgcaa ggttgggaag
     6601 ccggtggtca tcgcggtgtt ttcgatccgg ccagtcctga cagtcgcctc ggcacattgc
     6661 cgctgacgcg gctgctggtc caacgctgtg gcctcccgat catcgcggcc ggcgggataa
     6721 tggatggcgc agggatccgt gccgcactag accttggggc catggctgcc cagttgggaa
     6781 cggccttcgt cgcctgtccg gaaagcagcg ccgatgccgc ctaccgcgag gcgctggccg
     6841 gtccagccgc catggacaca gtcatgacct cagccatttc cggacggcct gcgcgcggtt
     6901 tggcgaaccg cttcaccgcc tggggcgaga gcgtcgggtc gccgcacccc ggctatccga
     6961 tgacctacga cgccggcaag gcactgatcg ctgccgccaa ggcagcaggc gagaccggct
     7021 atgctgctca atgggcggga cagggcgcgc ccctgtcacg cgcaatgcca gcggccgagc
     7081 tcgtggccgt cttggcaaag gaattgcatt tcgagagctg accggaactt ccgcgtcagg
     7141 gtgaccctgc ggattttacc gctggctcca gagccaccag tctgaaatct cctcgcgtga
     7201 catcgcccag cgtgtgctgg atgaagcgaa gctccccttc atcaggatgc tggaacaccc
     7261 gcacgccgcc ttcacgcgac atcacggcct gttcctccca gccgttgcga aaatgctcac
     7321 tgttctccag cagccaatca accactccag caacgcgcgg atcactcagg ccgcgtccat
     7381 aatcgcgccg gaactcggcc agcaggcgtc gggcccgctc tgcccagtct ggcaggagac
     7441 ggcgggcctc ggggctggta aacacgtagc gcagcaggtt gggctgggcc tcgccgtcga
     7501 acagcccgac aaacagccgc cgcgccggcg cattggcgca gcagacggtc catgccgcat
     7561 ccagcccata ggcgggccag tccaggcggc tgaccagtgc cgtgatagcc agaggggccg
     7621 cccccggcgc ttgcgcaaac gggtcggccg ggtcgcggtg ccccgcgagc gagaagaggt
     7681 aagtcctttc tgcctgggtc aggtccaggg agtcggcgag ccgtcccatc gtctccgccg
     7741 aagcgcggac cgcccggccc tgttcgatcc acgccaccca ggtcgcgcct atccccgccc
     7801 gagcggccag ttcctcgcgg cgcagcccgg gagtcctgcg cctccgtccc ggcgtatcgg
     7861 gcacgatggc ctcgcgccgg gcgcgcacga acgcccccag cagcttgcga tgatcttcgt
     7921 ccagcatgtg agagcttata acagaataaa gttgtccatt gtaactggct aatcactgcg
     7981 gcaccccggg gaaaaggaga atctcatgat gaccccgcac aacagtttgg tcggtaaaca
     8041 gttcggcgtt gccgctgcgg actacgtcac cagcacggtt catgccgagg gaccggattt
     8101 gcgccggatc gccgaagtgg cggcggctca cgcgccccgc cgcgcgcttg atctcgggtg
     8161 cggcgggggg catgttgcct acgccctcgc cccgcattcc ggcgaggtaa tcgccagcga
     8221 tctctccgcc gagatgaccg ccgcggtggc ggccgaggct gcccgccgcg gccttggcaa
     8281 tatccacacg gtcacggccc ctgccgaagc cttggggttc cccgaccaat cgttcgatct
     8341 gcttgcctgc cgtttcaccg tccaccactg gcgcgatgct gcagccggcc tgcgcgaagc
     8401 gcggcgagtc ttgcggcggg gggctcccgc cctgttcgcc gatgtgattg cccccgacct
     8461 cgccgctgcc gatacccacc tgcaagcggc cgaactgctg cgcgacccct cgcacgggcg
     8521 cgattatcgc cttggcgagt ggcgaacgat gctcgaacag tcaggcttca ggatcaccgc
     8581 gatcaccacc ggccagctcc gcatggactt cgccgactgg accgcgcgga tgcgcaccac
     8641 accggaccgg gtggcggcga tccgggcctt gcaaacgctg gccagcgatg aggtcgcaca
     8701 ccatttcgca atcgaagctg atggctcgtt cacgatcgac acggccctga tcgaagcctg
     8761 ctagggccgc catagcgagg gcgaactggc gcacttccgc ctgttccgac ctaagcgccc
     8821 agagtgccgc ccggtttatc gacagcaggt gtgccaaaca ggccagccga gaggtagccg
     8881 ccgtccaccg ccagggtctg tccgctgaca tagctcgaca tatcggagag gaagaatgtg
     8941 atggccttgg cgatttcatc ggtcgtcccg cggcgtccca tcggaatgca agccgacatc
     9001 tgcctctcaa ggcccgcctc gcgctcgggg atgcgcggcg tgatgattgc gcccggggcg
     9061 acgaggttga ctcgaatgcc atgctccgcc cattccatcg tcattgaacg gacgagattc
     9121 atcaggcctg ccttggctgc gccataggaa gcgtggtgcg gcgcagagcg gatgccgtcg
     9181 accgagcaga ccccgacgat cgacccgggc atccctcgcg acactagttt gcgggcgact
     9241 gcctgtgcaa tcatgaagaa gtagcggaga ttgcgctgct gatccatgtc ccaggtctga
     9301 ggtgtcatgt cgagcaagct gctccacccg gccattccga cgatcgccgc catgccgctc
     9361 agcgggccga tcgcctcttc cgcctcgtcg accgccctac caacagcgcc atcgtcgaac
     9421 acatcgccaa caatggcagc gctgcgcacg ccttccttcg cggcctcggc cgccgtgctc
     9481 tcggcgcgct cgcgatccac atcgaggaca gcgacatcgg cccccagcga tgcgagaagc
     9541 cgaacagtgg acgcgcccat tc
//
LOCUS       DATNBV010000206         3030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2977796,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000206 DATNBV010000000
VERSION     DATNBV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2977796"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VN627_05045"
     CDS             complement(<1..359)
                     /locus_tag="VN627_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02658.1"
                     /translation="MQQQAVDQVEAVLKDLVESGQGIESARKPLKEIALANGWDWFVG
                     KTAADLGRNLGPLVRAYSYASYSDDRRQQLLDEEQGAWIFHAGMACDEHHQLLDGLCL
                     EPGHPFWSRHSPPHGWD"
     gene            complement(544..1088)
                     /locus_tag="VN627_05050"
                     /pseudo
     CDS             complement(544..1088)
                     /locus_tag="VN627_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051771.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
     gene            complement(1156..1815)
                     /locus_tag="VN627_05055"
     CDS             complement(1156..1815)
                     /locus_tag="VN627_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HXD02659.1"
                     /translation="MTKHDGLIKGWSRDQMAARVAQELRDGYYVNLGIGIPTLVANHV
                     PAGITVTLQSENGMLGIGPFPTEAEIDADLINAGKQTISQLPHSAYLDSAQSFAMIRG
                     GKIDLTVLGAMEVAENGDIANWMIPGKMIKGMGGAMDLVAGVKRIIVVMEHCAKDGSA
                     KFIPACTLPLTGRNKVDMLVTDLAVFQRPDHASPFRLMECAPGVTAGEVRAKTTAHYV
                     E"
     gene            complement(1815..2534)
                     /locus_tag="VN627_05060"
     CDS             complement(1815..2534)
                     /locus_tag="VN627_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HXD02660.1"
                     /translation="MNKVYPDARAALDGLLNDGMLIAAGGFGLCGIPERLLDAILASG
                     VTGLTFASNNAGIDNEGIGKLLRTRQVKKMIASYVGENKEFERQYLAGELEVEFCPQG
                     TLAERMRAGGAGIAGFFTKTGVGTLVAEGKQSQEFDGPDGPEEYILERGIVADLAVVK
                     AWKADTTGNTVFRKTARNFNVPAATCGRVCVVEVEEIVEPGQLDADAIHLPGVYVQRI
                     VLGAPYDKKIEFRTTRSTDVG"
     gene            2741..>3030
                     /locus_tag="VN627_05065"
     CDS             2741..>3030
                     /locus_tag="VN627_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXD02661.1"
                     /translation="MRSILVHAETGPAGRARIDTALSLARATGGHVTLLVDTPVSRYV
                     AVDGLGGGFVATDALKDAIADDDAFARQMDEHLARGDVCCDVLRAERDPIDAL"
BASE COUNT          484 a          964 c         1004 g          578 t
ORIGIN      
        1 cagtcccacc cgtgcggtgg cgaatgccgg ctccagaacg gatggccagg ctcaaggcaa
       61 agtccatcca gcagctggtg atgttcatcg caggccatcc cggcgtggaa gatccatgct
      121 ccttgctctt catcgagcag ctgctggcga cgatcatcgc tatagctggc ataggaatag
      181 gcccggacga gggggccaag gttgcgtccc aggtcggcgg cagtcttgcc gacgaaccag
      241 tcccacccat tggcgagagc aatttccttc agcggcttgc gcgcactttc gatcccctgc
      301 ccgctttcaa ctaggtcttt gagcaccgcc tcgacctgat cgacggcctg ctgttgcaac
      361 ggttccaatc ccctctcccc ctcaaatctt cttcacgaca gccaccacgc gcccgaacac
      421 gtgcaactcg ccatcgtagg ctgtttccgg cggtacggca gggttgtccg acaggggcgt
      481 tgcgcttcgc ggcgggcgcc cctccaccac cttcggtggc cccctccctc acgagggagg
      541 attttacttt gcgagttcgg ccttgattgc tccgacgagg tcagggtcgg agggttcggt
      601 gccaggggcg aagcgggcgg cgaccttgcc gtcgcggccg attaggaact tctcgaagtt
      661 ccacagcact tccgggtcct cggtcggcgt catcccgtag cccttgagcc gctcacggaa
      721 ttcctcggcc gggccgcgct tggtcggctc ggccgcggtc agtgcggcat agagcggctg
      781 cttggccggg ccggtaacat cggccttgca tatagcggga aagagacgcc gtagttgagc
      841 ttgcagaact cggcgatttc ctcgtgcgtg ccgggctcct gcgcaccgaa atcgttggcg
      901 ggaaagccga gcacctcgaa gccttcgtcc ttgaggccgg catagagctt ttccaggcct
      961 tcatactgcg gcgtgagccc gcatttgctg gcgacgttga cgaccagcag gaccttgccg
     1021 gcatgattgg caaggctgtc ggacttcccg tcgatgcggg tgagcgggat tgcggcgaga
     1081 tcggtcatgc aaggtctccg gctgttttgc cgggagacta tgcccaagga catgggcgcg
     1141 gcaagggggc tgccttcatt ccacgtagtg cgcggtcgtc ttggcccgca cttccccggc
     1201 cgtgacgccc ggcgcgcatt ccatcaggcg gaaggggctg gcatggtcag gccgctggaa
     1261 cacggcgagg tcggtcacca gcatgtcgac cttgttcctg ccggtgagcg gcagggtgca
     1321 ggccgggatg aacttggcgc tgccgtcctt ggcgcaatgc tccatgacga cgatgatccg
     1381 cttgaccccg gcgacgaggt ccatcgcccc gcccatgccc ttgatcatct tgcccgggat
     1441 catccagttg gcgatgtcgc cgttttccgc cacttccatg gcgccgagca cggtgaggtc
     1501 gatcttgccg ccgcggatca tggcgaagga ctgggcgctg tcgagatagg cggagtgggg
     1561 cagctggctg atggtctgct tgccggcgtt gatcaggtcg gcatcgatct cggcctcggt
     1621 cggaaagggg ccgatgccga gcatgccgtt ttcgctctgg agggtgaccg tgatccccgc
     1681 gggcacgtga ttggcgacca gcgtggggat gccgatgccg aggttcacgt agtagccgtc
     1741 acgcagttcc tgcgccaccc gggcggccat ctggtcgcgg gaccagccct tgatcagtcc
     1801 atcatgcttg gtcatcagcc gacatccgtg ctgcgggtgg tgcggaattc gatcttcttg
     1861 tcgtagggcg cgccgagcac gatccgctgg acgtagacgc ccgggaggtg gatcgcatcg
     1921 gcatcgagct gcccgggctc gacgatctcc tcgacctcga ccacgcagac ccgcccgcag
     1981 gtggcggcgg gcacgttgaa attgcgggcg gtcttgcgga agacggtgtt gccggtggta
     2041 tcggccttcc aggccttgac cacggcgaga tcggccacga tcccgcgctc gagaatatat
     2101 tcctcaggtc catctggccc gtcgaattcc tggctttgct tgccttcggc gaccagcgtg
     2161 ccgacgccgg tcttggtgaa gaacccggca atgccggcgc cgccggcgcg catgcgctcg
     2221 gccagggttc cctgcgggca gaattcgacc tcgagctcgc cggcgaggta ttgccgctcg
     2281 aactccttgt tctcgcccac gtaggaggcg atcatcttct tcacctgacg tgtgcgcagg
     2341 agcttgccga tgccttcgtt gtcgatcccg gcattgttgg aggcgaaggt caggccggtc
     2401 acgcccgagg cgaggatggc atcaagcagg cgttcgggta tgccgcaaag gccgaagccg
     2461 cccgccgcga tcagcatgcc gtcgttcagc agcccgtcca gcgcggcgcg ggcatcgggg
     2521 tagaccttgt tcacgtgacc ctccaggccg aaccccggtc aacggggcgg acctatggcc
     2581 tttaatgcag tgcagcatga tctgtcacgc atcatattcg cgatggagac gcaggcgccc
     2641 gatcgcccgg ccctgtggat tcgctgtcta attgattcag atcatggtaa aaatggccgg
     2701 cggctggaga acgaggacac caccaaaggg agacaggccg atgcgctcca tactggttca
     2761 tgccgaaacc gggcctgccg gacgggccag aatcgatacc gcgctctcgc tcgcgcgggc
     2821 gaccgggggg cacgtgacgc tgctcgtcga tacgccggtc agccgctatg tcgcggtcga
     2881 tggcctgggc ggcgggttcg tggccaccga tgcgctgaag gacgccatcg cggacgacga
     2941 cgccttcgcc cgacagatgg acgagcacct ggcgcgcggc gacgtgtgct gcgacgtgct
     3001 gcgggccgag cgcgatccga tcgacgcgct
//
LOCUS       DATNBV010000207         4045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2039762,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000207 DATNBV010000000
VERSION     DATNBV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2039762"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="VN627_05070"
     CDS             <1..1492
                     /locus_tag="VN627_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017180906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXD02662.1"
                     /translation="QPCGDFGRNGSVDLKEGMSKAPAGYYYVTSAPTVVRGKIIVGGW
                     VMDGMYWGEPSGVIRAFDGASGKLSWAFDVGRLDRTGAPPPGETYTPSTPNSWAPMSV
                     DEQLGLVFAPTGNATPDNFGGRRRPFDEEWSSAVVAIDAETGRPRWHFQTTHHDIWDY
                     DVASQPTLVDLPIGGRVRHALVQPTKRGEVFVLDRETGQPLMPVTEHPVPQAGIVPGE
                     RLSRTQPFSDAVASFRGVQMAERDMWGITPLDQLWCRIQFRQARYEGPLTPPGTSPSI
                     LFPGFLGGSNWGGVSIDRDRHLMIVNSNFVANYLRLIPRAMADRLGVRPVGMGGQPKP
                     EGQGVMAQDGTPYASRSLPFLSPIYAPCQAPPWGRISAVDLATGKLVWSHRLGTARDS
                     GPLGLPSLLPFKIGTPNLGGSVATRGGVIFIAATQDKYLRAIDTATGEVLWRGRLPNA
                     GQTTPITYLSPASGRQFVVTASGGHFLMGTAPGDAIYAYALPKNAR"
     gene            1627..2658
                     /locus_tag="VN627_05075"
     CDS             1627..2658
                     /locus_tag="VN627_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXD02663.1"
                     /translation="MTDTVGEKPRRLRNITELAKLAGVSPGTVSRALAGKNLVNPATR
                     DRIQALAREHGFRLNQMASRLRTGQTGVIGVVIPLGHERKQHISDPFFMALLGHLADQ
                     LVDRGYDLMLSRALPGDERHWLDRIIESGMLDGVLMIGQSDQFETIEEVAQRYRPLVV
                     WGAWREGQRHCVVGTDSFAGGRLAARRLIERGCRNLAFFGDTRGFEIDQRLAGFRAAA
                     AESGSGAMVTVYPTHLSASEMVSQIAADLDSMDPSTDGILAASDMIAIATLRQLHERG
                     RKVPGDVALIGFDDLPIATQTVPQLTTVRQDIAGGAQAMVNAVLRRIAGEDVPSTVMT
                     PHLVVRESA"
     gene            complement(2676..3539)
                     /locus_tag="VN627_05080"
     CDS             complement(2676..3539)
                     /locus_tag="VN627_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXD02664.1"
                     /translation="MIELMIEQRRRSLGGGLEVGRVLPVAQRRMVGPFVFFDHMGPMD
                     LAAGVDRAVDVRPHPHIGLSTVTYLFSGEVMHRDSVGSHQAIRPREVNWMTAGSGITH
                     SERFEKARAEGDHLHGIQAWVALPTADEECAPSFTHLAGADLPQWDEGGVSGQLIAGS
                     AYGLTATAPVYSPMFYAALVLAPGAKAELPSGYSERAIYVVTGAVELDGGRHPTGRML
                     VLGAGTAAIRSDEGATVMVLGGEPVGERFLYWNFVSSSQERIAQAAADWRAGRIKLPD
                     LDHDEFIPLPD"
     gene            3774..>4045
                     /locus_tag="VN627_05085"
     CDS             3774..>4045
                     /locus_tag="VN627_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02665.1"
                     /translation="MAVQTVMERVKETRDLGYPVYDADGHYYETMDTFRRHLPKKFHK
                     DFQYVQVEGRTKLAINGHISDYIPNPTFEVVAAPGSHEIWFRGENL"
BASE COUNT          651 a         1445 c         1313 g          636 t
ORIGIN      
        1 ccagccctgc ggcgacttcg gacgcaacgg gtcggtggat ctgaaggagg gcatgtccaa
       61 ggcgcccgcg ggttactatt acgtcacctc ggcgcccacc gtagttcgcg gcaagatcat
      121 tgtcggcggc tgggtgatgg acgggatgta ctggggcgaa ccctcgggcg tcatccgcgc
      181 cttcgatggc gcatcgggca agctcagctg ggcgttcgac gtcggccggc tcgatcgcac
      241 cggcgcaccg ccgccgggcg agacctacac cccctccacg cccaacagct gggcaccgat
      301 gagcgtggac gagcagcttg gactcgtgtt cgcccccacc ggcaacgcca cgcccgacaa
      361 tttcggcggc cgccgccgtc cgttcgacga ggagtggtca agcgcggtgg tcgccatcga
      421 tgccgaaacc ggccgccccc gctggcactt ccagaccacc catcacgata tctgggacta
      481 cgatgtcgcc tcgcagccca cgctggtcga tctcccgatc ggcggccggg tgcggcatgc
      541 cctggtgcag ccgaccaagc gcggggaagt cttcgtgctc gaccgcgaaa ccgggcagcc
      601 gctcatgccg gtcaccgaac accccgtgcc gcaagccggc atcgtcccgg gtgagcggct
      661 ctcccgcacc cagccgtttt ccgacgccgt ggcctcgttc cgcggcgttc aaatggccga
      721 gcgcgacatg tggggcatca ccccgctcga ccagctgtgg tgccgcatcc agttccgcca
      781 ggcccgctac gaaggtccgc tgaccccgcc gggaacctcg ccctcgatcc ttttccccgg
      841 cttcctcggg ggctccaact ggggcggcgt gtcgatcgac cgcgaccggc acctgatgat
      901 cgtcaacagc aatttcgtcg ccaattacct gcgcctgatc ccgcgggcga tggccgatcg
      961 gctcggcgtc aggccggtgg gcatgggggg gcagccaaag cccgaagggc agggggtcat
     1021 ggcccaggac ggaacgccgt atgcgtccag gtccctgccg ttcctctccc cgatctacgc
     1081 tccgtgccag gcgccgccgt ggggccggat cagcgcggtc gaccttgcca ccggcaagct
     1141 cgtctggtcg caccgcctgg gcaccgcgcg tgacagcggc ccgctcggcc tgccctcgct
     1201 gctgcccttc aagatcggca cgcccaatct tggcggctcg gtcgccacgc gcggcggcgt
     1261 gatcttcatc gccgcgacgc aggacaagta cctgagggcc atcgacacgg cgaccggcga
     1321 ggtcctgtgg cgcggccggc tgcccaatgc gggccagacc acgccgatca cctatctgtc
     1381 tcccgccagc ggccggcagt tcgtggtgac ggcctcaggc ggccatttcc tgatgggcac
     1441 ggctccgggc gacgcgatct acgcctacgc cttgccgaag aacgcccgct aggcagaccg
     1501 gaggccctcg ccgcgcagcg gcgggggcgc cagcctgttg cgaattgccg gcgcgatgcg
     1561 tagcctcaag cgattgcagg tccggcgggc gttgtcgcgg cgccggacgt tagatgggtg
     1621 tcacgaatga ccgacacggt cggggagaag ccgcggcggc ttcgcaacat cacggaactg
     1681 gccaagctcg ccggcgtgtc cccgggcacc gtgtctcgcg ctctggccgg caagaatctc
     1741 gtcaaccccg ccacgcgcga ccgcattcag gccctcgcgc gcgagcacgg gtttcgcctc
     1801 aaccagatgg ccagtcgcct gcgaaccggg cagaccgggg tgatcggggt cgtcatccct
     1861 ctcggccatg aacgcaagca gcacatttcc gacccgttct tcatggccct gctcggacat
     1921 ctcgccgacc agctggtcga ccgtggctac gatctgatgc tcagccgcgc cctgcccggc
     1981 gacgagcgtc actggctgga ccggatcatc gaatccggca tgctcgacgg cgtcctgatg
     2041 atcggccagt cagaccagtt cgagacgatc gaggaagtgg cccagcgtta ccgtccgctg
     2101 gtcgtctggg gagcgtggcg cgaggggcaa cggcactgcg tcgtcgggac cgacagtttt
     2161 gcgggaggcc gcctcgccgc gcgccgcctg atcgaacgcg gctgccgcaa ccttgccttc
     2221 ttcggcgata cccgcggctt cgagatcgac cagcgcctcg ccggcttccg cgccgctgcc
     2281 gccgaatccg gctccggggc gatggtcacg gtctatccca cccacctttc ggccagcgaa
     2341 atggtcagcc agatcgccgc cgatcttgac tccatggacc cgtcgaccga cggcatcctc
     2401 gccgcgtccg acatgatcgc gattgccacc ctgcgccagc ttcacgaacg cgggcgcaag
     2461 gtgcccggcg acgtcgccct gatcgggttc gacgacctcc cgatcgccac ccagaccgtc
     2521 ccgcagctca ccaccgtgcg ccaggacatt gccggcggcg cccaggccat ggtcaacgcc
     2581 gtgctgcgcc ggatcgccgg cgaggatgtg ccgtccaccg tgatgacccc gcacctcgtg
     2641 gtgcgcgaga gcgcctgaac gggctgcccc gctggtcagt cgggcagcgg aatgaactcg
     2701 tcgtgatcga ggtcgggcag cttgatgcgc ccggcccgcc agtcagccgc agcctgggcg
     2761 atccgctcct gcgaggacga gacgaagttc cagtagagga agcgctcacc gaccggctct
     2821 ccgccgagca ccatcaccgt cgccccttcg tccgagcgga tcgccgcggt ccccgcgccg
     2881 agcaccagca tccgccccgt cgggtggcgg ccgccatcca gctcgaccgc gccggtgacg
     2941 acatagatcg cccgttcgct gtatcccgac ggcagctcgg ccttggcgcc cggggcaagg
     3001 accagcgcgg catagaacat cggcgaatag accggagcgg tggcggtcag cccataggcg
     3061 ctgccggcga tcagctggcc gctcaccccg ccttcgtccc attgcggcag gtccgcgccg
     3121 gccaggtggg taaacgatgg cgcgcattcc tcgtccgccg tgggcagggc cacccaggcc
     3181 tggatgccgt ggagatggtc gccttcggcc cgggctttct cgaaccgttc gctgtgggtg
     3241 attccgctgc cggcggtcat ccagttgacc tcgcgcgggc ggattgcctg gtgcgagccc
     3301 acgctgtcgc ggtgcatcac ctcgccggag aacaggtagg tgacggtgga cagcccgatg
     3361 tgcggatggg gccgcacgtc gaccgcccgg tccacgccgg cggcgagatc catgggcccc
     3421 atgtggtcga agaacacgaa cgggccgacc atccgccgct gcgcgaccgg cagcacccgg
     3481 ccaacctcca gcccgccccc caggctgcgg cgccgctgct cgatcatcag ttcaatcatg
     3541 gaaatgctcc ggcgggaggg aagggcaccg cgattgttgc acctgccgta tgcaagtcaa
     3601 agacttgtcc gcacgggatg ggcttctcgt cgggcgccga ggtctttttc caggcgaatt
     3661 tccgcaggaa ttgacgtata gtcggaaaaa tcggtccagg actaaagccc gggcgattcg
     3721 ccgcgcggcg aaaccgccgc gtctggccaa aaaataaacg acaggagatc agcatggcag
     3781 ttcagaccgt tatggaacgg gtcaaggaga cccgcgatct tggctatccg gtctacgacg
     3841 ccgatggcca ctactatgag acgatggaca ccttccgtcg ccacctgccg aagaagttcc
     3901 acaaggactt ccagtacgtt caggtggaag gccgcaccaa gctggcgatc aacggccata
     3961 tctcggacta tatccccaac cccaccttcg aggtcgtggc cgcccccggt tcgcacgaga
     4021 tctggttccg gggcgagaac ttgga
//
LOCUS       DATNBV010000208         3527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_133507,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000208 DATNBV010000000
VERSION     DATNBV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133507"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VN627_05090"
     CDS             complement(<1..589)
                     /locus_tag="VN627_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02666.1"
                     /translation="MGGEVKRRADGGWPLDAIPRASGPLGPTPPRRPWSVRRTTSIDT
                     VWPGGIGTDLHMSGRGRDLLTGADPSHPEVLAEDSFTARLSRAREILELDVTPGRAGA
                     ATLVGSRAGGQLRSAVAEALPGELASASPLYLLLDDMAGASLVAAWAWSQWADDWMQR
                     LHSAAEAELKEPRHHKMIGVCVGFSPQSPVVLEGGP"
     gene            complement(589..1440)
                     /locus_tag="VN627_05095"
     CDS             complement(589..1440)
                     /locus_tag="VN627_05095"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HXD02667.1"
                     /translation="MIDQCASDQVTEEDAEARKHAAWGRVIRMEAINNFRDYGDLPVS
                     GGGRVRKGRLFRSAHLARASAADLAKLADLGVATVTDLRHPSEQELQPGAWIGRLPLA
                     VIEEPAPEDQEAHEAPHIAAFRESNFSAADLREFLGGHYRIMPFDPRHVAMFRRYFAG
                     LAGQDGGMLIHCAAGKDRTGILAWLTHHVLGVHPDDAMADYLLSNDAANIAERLPAVR
                     ARLQETYGRPISEEAMYGMLTVTPEFIASLIDALTENAGSVDAYLADVVGLDAAGRDA
                     LRARLIV"
     gene            1653..3293
                     /locus_tag="VN627_05100"
     CDS             1653..3293
                     /locus_tag="VN627_05100"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD02668.1"
                     /translation="MAETISNSGETARVSMWAPLAFPLFRNIWSASLLSNLGFMFLSV
                     GAGWQMTLLTPRADMVALVQTALMLPMMFLAIPAGAMADMYDRRKVALGALCVSLASG
                     FSLAALAALHLLTPWLVLGFCFLVGLGMALYAPSWQASVSEQVPRETLSEAVGLTAIS
                     FNIARSVGPALGGALVAAAGPVAAFTGTAFGYVPLFAAMLMWKRKAVPSRLPPERIDR
                     AVGAGVRYVLHSPPIRTVVLRAFATTFTGCSISALMPLIVRDQLHSSAEVFGLLLGCF
                     GVGAVIGGLILPGLRRRFAEEAIVSGVSVILGTAIAAIALSPWYLLTAAVLVVAGICW
                     TISITIYNVVIQTAAPRWVSGRLVASFQTATAGGTACGAWFWGHIAGTAPIHDTLLIS
                     AGAVAASALLGLLLKVPSASDSEHEEVRLADPEVNLDLTGRSGPIVVEMEYRITREDA
                     REFYRRMMAVRLVRMRNGAFEWSIARHITDPEIWVERYSCPTWHDYLRLRDRNTAEEM
                     LIMQHAQAMIRPGHAVVIHRMLERPFGSVRWFENAPEV"
     gene            complement(3298..>3527)
                     /locus_tag="VN627_05105"
     CDS             complement(3298..>3527)
                     /locus_tag="VN627_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02669.1"
                     /translation="LKVMRRMVQITGGRGFDYGATPTAAHIGPSTGACYTFQPERAAE
                     ALLTMDKRDFSFGPPPLAGLIAEVMERPAWK"
BASE COUNT          528 a         1268 c         1156 g          575 t
ORIGIN      
        1 tgggcccgcc ttcgaggacc accggcgact gcgggctgaa ccccacgcag accccgatca
       61 tcttgtggtg ccgcggctcc ttcagctcgg cctcggccgc gctgtgaagg cgttgcatcc
      121 agtcgtcggc ccactgcgac caggcccagg ccgcaacaag gctggccccg gccatgtcgt
      181 caagcagcag gtagagcggc gaggcgctgg ccagctcgcc cggcagggcc tcggccacgg
      241 ccgagcgcaa ctgcccgccc gcccgcgagc ccaccagcgt cgccgcgcca gcccgccccg
      301 gcgtcacatc aagctcaagg atctcgcgcg cccgcgaaag cctggcggta aaactgtctt
      361 cggccagcac ctccggatgg ctgggatcgg ccccggtcaa gaggtcccgg ccccggcccg
      421 acatgtgcag gtcagtgccg atgccgccgg gccagacggt atcgatcgag gtcgtccggc
      481 gaaccgacca gggccggcgc ggcggggtcg gcccgagcgg gccggacgcc ctgggaatcg
      541 catcgagcgg ccagccgcca tcggcccggc gcttcacctc gcctcccatc agacgatcaa
      601 ccgcgcgcgc agggcgtcgc gcccggcggc atccaggccg acgacatcgg caagataggc
      661 gtcaacggac ccggcgttct cggtgagggc atcgatcagg ctggcaatga actcgggcgt
      721 aaccgtcagc ataccgtaca tggcttcctc ggaaatgggg cggccgtagg tctcctgcag
      781 ccgggcgcgc accgccggca ggcgctcagc gatgttggcc gcatcgttgc tgaggaggta
      841 gtctgccatg gcatcgtcgg gatggacgcc caggacatgg tgggtcagcc atgccaggat
      901 gccggtgcga tccttgcccg cggcacagtg gatcagcatg ccgccgtcct gtccggccag
      961 tcccgcgaaa tagcgccgga acatggccac gtggcgcgga tcgaagggca tgatccggta
     1021 atgcccgccc aggaattcgc gcaggtcggc agcgctgaaa ttgctttcac ggaaggcggc
     1081 gatatgcggc gcctcgtgag cttcctgatc ctccggggcc ggttcctcga tcacggccag
     1141 cggcaggcgc ccgatccacg cacccggctg aagctcctgt tcgcttgggt ggcgcaggtc
     1201 ggtcaccgtg gcgacgccga ggtcggccag cttggcaagg tcggccgcgc tggcccgcgc
     1261 cagatgggcg gaacggaaca gccgcccctt gcgcacgcgc ccgccgcccg acaccggcag
     1321 atcgccatag tcccggaagt tgttgatcgc ctccatccgg atcacccggc cccaggccgc
     1381 gtgcttgcgc gcctcggcat cttcttccgt cacctgatca gaggcgcatt gatcgatcat
     1441 cacaaactcc cgcggcgcgc tcgaaccgcg tcgaatatga tgatggtccg ggcagcccca
     1501 ctgttcaagg ccgcccaagg ggtggcgccc tttcgatcac atgtcggatt tccctcgcga
     1561 aagctggtgt gaaacggcgt ttcgggggtt cgacagcccg gtccgaagca gatagaggat
     1621 cggccccgag gcaatgaaca gggcacgatc aggtggcgga gacgatcagt aacagtgggg
     1681 aaaccgcccg ggtgagcatg tgggcgccgc tcgccttccc gctgttccgc aacatctggt
     1741 cggccagcct gctgtcgaat ctcggcttca tgttcctctc ggtcggcgcc ggctggcaga
     1801 tgaccctgct caccccccgg gctgacatgg tcgccctggt ccagaccgcg ctgatgctgc
     1861 cgatgatgtt cctggcgatc cccgccgggg ccatggccga catgtacgac cgccgcaagg
     1921 tcgcgctggg cgcgctgtgc gtctcgctcg cctcgggctt ctcgcttgcc gccctcgccg
     1981 cgctccacct gctcaccccg tggctggtgc tcggcttctg cttcctcgtc ggccttggca
     2041 tggcgctcta tgcgccgtcc tggcaggcct cggtcagcga acaggtcccg cgcgaaaccc
     2101 tgtccgaggc cgtcgggctc accgcgatca gcttcaacat cgcgcgcagc gtcggcccgg
     2161 ccctgggcgg cgctctggtc gcggcggcag ggcccgtggc cgcctttacc ggcacggcct
     2221 tcggctatgt cccgctgttc gccgccatgc tgatgtggaa acgaaaggcc gtgccctcgc
     2281 gccttccgcc cgaacgtatc gaccgcgcgg tgggcgccgg cgtgcgctac gtcctccact
     2341 cgccccccat tcgcaccgtc gtcctgcgcg ccttcgccac caccttcacc ggctgctcga
     2401 tcagcgcgct gatgccgctg atcgtgcgcg accagctgca cagctcggcg gaagtgttcg
     2461 gcctgctgct cggctgcttc ggggtcggcg cggtcatcgg cggcctgatc ctgcccgggc
     2521 tgcgccgccg gttcgccgag gaagccatcg tctcgggtgt ctcggtcatc ctcggcacgg
     2581 caatcgccgc gatcgcgctc agcccgtggt acctgctgac cgccgcagtc ctggtggtcg
     2641 ccgggatctg ctggaccatc tcgatcacga tctacaacgt cgtcatccag accgccgctc
     2701 cccgctgggt ctcgggccgc ctcgtcgcct cgttccagac ggcgaccgcc ggtggcacgg
     2761 cctgcggcgc atggttctgg ggccacatcg ccggcaccgc cccgatccac gataccctgc
     2821 tgatttccgc gggcgccgtc gcggcttcag ccctgctcgg cctcctgctc aaggtgccca
     2881 gcgcctcgga cagcgagcac gaagaagtcc gcctggccga cccggaggtc aaccttgatc
     2941 tcaccggccg cagcgggccg atcgtggtcg agatggaata ccggatcacc cgcgaggatg
     3001 cgcgcgagtt ctaccggcgg atgatggccg tgcggctggt gcgcatgcgc aatggcgcct
     3061 tcgaatggtc gatcgcgcgg cacatcaccg atccggaaat ctgggtggag cgatactcct
     3121 gcccgacctg gcacgattac ctgcgcctgc gcgatcgcaa caccgccgag gagatgctga
     3181 tcatgcagca tgcccaggcc atgatccgcc cgggccacgc cgtggtcatt catcgcatgc
     3241 ttgagcgccc cttcggctcg gtccgctggt tcgaaaacgc gccggaagtc tgaagcatca
     3301 cttccacgca ggccgttcca tgacctcggc gatcaaaccg gcaagcggcg gcggaccaaa
     3361 cgagaaatcc cgcttgtcca tcgtcagcag agcttcggcg gcgcgctcgg gctggaaggt
     3421 atagcaagcc ccggtcgacg ggccgatgtg agcggcggta ggggtggcgc catagtcgaa
     3481 cccgcggccg ccggtgatct ggaccatccg ccgcatgacc ttcagga
//
LOCUS       DATNBV010000209         3104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3313731,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000209 DATNBV010000000
VERSION     DATNBV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3313731"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VN627_05110"
     CDS             <1..643
                     /locus_tag="VN627_05110"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD02670.1"
                     /translation="PAFPNSLEVLRNGGAGAVQSLALMGDPAGPRMYSHPEFDPFWQA
                     VCDLDMPVTIHLGGRRVRIDQMEHYLTDLVMSKLAMAEPISVLVFGQVFERFPALKFV
                     TVESGAGWLAFVADYMDRTWEKQRFWVKSELKDYPSTYMDRNVYASFIHDSIGIATRH
                     LPGAGNIMWSSDYPHSETTYPHSADMIERLMGKIGENDRAEIVGGRAARVFGI"
     gene            705..1775
                     /locus_tag="VN627_05115"
     CDS             705..1775
                     /locus_tag="VN627_05115"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXD02671.1"
                     /translation="MTWYVRNAWYAAAFENELDGEFLARTICDVPLVLFKRSDGTVAA
                     IHDKCPHRFVPLSMGKRVGDTMQCGYHGLRFAGDGSCAEMPFEHGQDRPDVCVDAYQV
                     VLRDTIAWLWMGDADKADPALIPDFWPLRDPEMQTVRGHSRFACNYQILCDNLLDLSH
                     IHYLHPGVHDGSNFANFRNEVSVEGHTIWSLLYRPGYVLDETKRKAWRIDADTCDGQG
                     HSRWTVPGVLLVDTAYWPEGSDISQGAARMPSTHLLTPETEFSTHYLWGSSRNFGPVD
                     PASVKASEEMIRAIFETQDGPMAEAQQRAMGRETDLLALRPAILKADKAGVLARRLIQ
                     RLVREQNSLPEPASERPLTAAV"
     gene            2083..>3104
                     /locus_tag="VN627_05120"
     CDS             2083..>3104
                     /locus_tag="VN627_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXD02672.1"
                     /translation="MAENMTSADGNRDKPREKPRFVRRPFLFAALLILIGGGLIWGGV
                     QLASLGGSPYYLAAGLATLASGVLILSGRAVGALVYGLMLLATVAWAVGEVGSDPWQL
                     AARVTAPVVLGLWLLLPWTRAGLGLSRRIGRGAAVKALGATVGAVAIGFAAHALFVPT
                     RADPMYQAGVTGLPAATVSAASTGAAAADWPSWGGDPGGTRFSALDQITPKNADKLEL
                     AWTYHFGPPPPGAPKSLEVTPLKVGDTLYLCNDYNDVVALDAETGAQRWRFRAHTDTA
                     GATYGHCRGVAYLRSAATTGACAARIYTATVDARLLAIDAATGQLCGDFGRNGAVDLK
                     EGMSKAP"
BASE COUNT          514 a         1064 c         1015 g          511 t
ORIGIN      
        1 cccggccttt ccgaactcgc tcgaggtcct tcgcaacggc ggggccggcg cggtgcagtc
       61 actggccctg atgggcgatc cggccggccc gcgcatgtac agccacccgg aattcgatcc
      121 gttctggcag gccgtctgcg atctcgacat gcccgtcacc atccatctcg gcggccgccg
      181 ggtcaggatc gatcagatgg agcattatct gacagatctg gtaatgtcga agctcgccat
      241 ggccgagccg atctcggtgc tggtcttcgg gcaggttttc gaacggttcc ccgccctcaa
      301 gttcgtgacg gtcgagagcg gcgcgggatg gctggcgttc gtggccgatt acatggaccg
      361 gacttgggaa aagcagcggt tctgggtaaa gagcgaactc aaggattacc cgagcaccta
      421 catggaccgg aacgtctatg cgtcgttcat tcatgacagc atcggcatcg ccacccgtca
      481 cctgccgggc gcgggaaaca tcatgtggtc gtccgattac ccccacagcg agacaaccta
      541 tccgcattcg gcggacatga tcgaacggct gatgggcaag atcggcgaga atgatcgcgc
      601 cgagatcgtc ggcgggcggg cggcccgggt gttcggtatt tgaattggct ccagatgggg
      661 ctcggccccc atcgggccgg tggattggaa cggagagacg tgagatgacc tggtacgtgc
      721 gcaacgcctg gtatgcggcc gccttcgaaa acgaacttga cggcgagttc ctcgcccgga
      781 ccatctgcga tgtgccgctt gtcctgttca agcggtccga tggcacggtg gccgcgatcc
      841 acgacaagtg cccccatcgc ttcgtccccc tctcgatggg caagcgcgtg ggcgatacga
      901 tgcagtgcgg ctatcacggg ctgcgcttcg ccggcgacgg gtcctgcgcc gaaatgccgt
      961 tcgagcatgg ccaggaccgc ccggacgtgt gcgtcgacgc ctaccaggtc gtactgcgcg
     1021 atacgatcgc gtggctgtgg atgggcgatg ccgacaaggc tgatccggcg ctcattccag
     1081 atttctggcc cttgcgcgat cccgagatgc aaaccgtgcg cggccactcg cggttcgcat
     1141 gcaattacca gatcctgtgc gacaacctgc tcgacctcag ccacattcac tacctgcacc
     1201 ccggcgtcca tgacggctcc aatttcgcca acttccgcaa tgaagtgagc gtcgaaggac
     1261 atacgatctg gtcgctgctc taccgcccgg gctatgttct ggacgagacg aagcgcaagg
     1321 cctggcggat cgacgcggac acgtgcgacg gtcagggtca ctcgcgctgg accgtgcccg
     1381 gcgtcctgct ggtcgatacc gcctactggc cggaagggag cgacatcagc cagggcgcgg
     1441 cgcggatgcc ctccacccac ctgctcacgc cggaaaccga gttcagcacc cactacctgt
     1501 ggggctcctc gcgcaatttc ggacccgtgg acccggcgtc agtcaaggcc agcgaagaaa
     1561 tgatccgggc catcttcgaa acccaggacg gaccaatggc cgaggcccag cagcgagcga
     1621 tgggccgcga aaccgacctc ctggccctgc gtcccgccat cctcaaggcc gacaaggccg
     1681 gcgtgctcgc ccgccggctc atccagcggc tggtccgcga gcaaaacagc cttcccgaac
     1741 cggcaagcga acggccgctc accgcagcgg tgtagctctg ccgcgcatcc gcgcgagggc
     1801 ccggagcgag ggtagggagg aggaagcctc ccaccgccca acccggcgtg gccagcccgc
     1861 gccagcatta acagccacgc cttgctcgcc ccggctccca cgtgggcaaa gccgcacagg
     1921 ccgagcgcca gcgtcagggc gaatgccacc cagaggacag atgcaatgtg ggcggcgcag
     1981 gtcagccggg cttgcaaagt ttggctgctt gtgattgcga gccaccacgt aaccggacac
     2041 ggcaggtttg attgcctgat ccggtcgccc gagggaggga aaatggccga gaatatgacg
     2101 tcagccgacg gcaatcgcga caagcctcgc gaaaaacccc gattcgtgcg ccggccattc
     2161 ctgttcgcgg ccttgctgat cctcatcggc ggcgggctca tctggggcgg ggtccaactg
     2221 gcgagcctcg gtggttcgcc ctattacctc gccgccgggt tggcgaccct cgccagcggt
     2281 gtgctgatcc tgtccggccg cgctgtcggg gcgctcgtct atgggctgat gctgctcgcc
     2341 accgtcgcct gggccgtggg tgaagtcggc agcgatccgt ggcagctcgc cgcgcgcgtg
     2401 accgcaccgg tcgttcttgg cctgtggctg ctgctgccgt ggacccgggc cggtctcggg
     2461 ctctcgcgcc ggatcggccg gggcgcggcc gtcaaggcgc ttggggccac cgtgggcgcc
     2521 gtggccatcg gatttgccgc tcatgccctg ttcgtgccga cgcgggccga tccgatgtac
     2581 caggccgggg tgaccggcct gcccgcggcc accgtatcgg ccgcgtccac cggcgccgcc
     2641 gcggccgatt ggccctcgtg gggcggcgat ccgggcggca cgcgcttcag cgcgctcgac
     2701 cagatcaccc cgaagaatgc cgacaagctg gaacttgcct ggacctatca cttcggcccc
     2761 ccgccgcccg gtgcgcccaa atcgctcgag gtcacgccgc tcaaggtggg cgacaccctg
     2821 tacctgtgca acgactacaa cgatgtcgtc gccctcgatg ccgaaaccgg ggcgcagcgc
     2881 tggcgcttcc gcgcccacac cgacacggcg ggcgccacct atggccactg ccgcggggtg
     2941 gcctacctgc gctcggccgc aaccaccggc gcctgtgccg cgcggatcta taccgccacg
     3001 gtcgacgccc gcctgctcgc catcgatgcc gcgaccggcc agctctgcgg cgatttcgga
     3061 cgcaacggcg cggtggatct gaaggagggc atgtccaagg cgcc
//
LOCUS       DATNBV010000210         4492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1392533,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000210 DATNBV010000000
VERSION     DATNBV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392533"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..490
                     /locus_tag="VN627_05125"
     CDS             176..490
                     /locus_tag="VN627_05125"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXD02673.1"
                     /translation="MVRHSLAIASLLLALSAGTASAGIISAPALPAGSSYLTTVATAT
                     QMTVAHAYAHLREKPTTKSALLATLKHGTKVEVLDKVDDGKWTHVKVGSKEGYISTSL
                     LK"
     gene            661..1818
                     /locus_tag="VN627_05130"
     CDS             661..1818
                     /locus_tag="VN627_05130"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HXD02674.1"
                     /translation="MALVLGWLTAFGPLAIDTYLPSFPAISRDLGVEASSVEATVAVY
                     FLGLALGQSFYGPMADRWGRRPPLYAGLLLFILASAGCALTQSIGALIALRFLQALGG
                     SAQIVVARAVVRDHFDERESARVLSLLMLVMGVAPILAPLLGGQLVGFFGWRSIFWTL
                     AAFATACLVAVSFTLPESLPPERRLRQSLATVFRLYGNLLADRQFMAPVLAGSLISAG
                     MFAYIAGSPFVFMEIYGVPPAHFGLFFGSNALGLIAASQINARLVRRYAPARILTIVL
                     IVTACAGLVLLGTAATGIGGFPGLLVPLFLFVASLGFVAPNATALAMGPQGRNAGSAS
                     ALLGVTQFLCGGISGGLVSALYDGTPVPMVLVIALCGLGACGIHLLFGRRR"
     gene            complement(2084..3244)
                     /locus_tag="VN627_05135"
     CDS             complement(2084..3244)
                     /locus_tag="VN627_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXD02675.1"
                     /translation="MNIPTMSETFALGGFLARWSPRVRHDLSGSEAATLTLSALLELA
                     GPEDRRRWQELGLGYTDPRGASWLRSAIAERYDKLDDTHIVCCAGAQEVLTSTMRALL
                     APDDHAVVVVPIYQPSERAVTSICAATGVPLEDRGAWLLDVDRVASALQGNTRLVLMN
                     FPNSPTGASIDPAALAALVALCRRHGLWLVNDEVYGLVDLDPHLRSPRLADIYERGVS
                     INAVSKGFGLPGLRVGWVACQDRRFLAEVQLVKSMLSSCLAAPSEVLTHIALGAENRI
                     VERNKAILGSNRELVELFLDRHLEAFEGQASANSAFVCPRYLGSDGADRFAVELAREA
                     GVLLLPSGLWQSTLAETPGDRVRIGLGRVGVGAALQALSGYLVRRRRVAAAS"
     gene            complement(3241..3906)
                     /locus_tag="VN627_05140"
     CDS             complement(3241..3906)
                     /locus_tag="VN627_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010188629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXD02676.1"
                     /translation="MIPTLNEAATIGAVIRGIPRDFSADIIVADGGSVDATRDAAREA
                     GARVIDAGRGYGRACAAGAAAADPACRVIAFLDGDGADRGDLIARIAGPVLAGSHDFV
                     LASRTRGQREPGAMLWHQVLAGRVAGLGIGALYGMRYSDMCAFRAIDHAVLQRLGMRE
                     MSYGWNIEMQMRAARAGLRILEVPLPYRCRAGGASKVAGSLRGTLRAGGQIISTFLRV
                     AGR"
     gene            complement(3912..>4492)
                     /locus_tag="VN627_05145"
     CDS             complement(3912..>4492)
                     /locus_tag="VN627_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010188627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HXD02677.1"
                     /translation="GKFVAYQRDVGATTPRVSLWLTGLKETIDQLPAFVRLAAIMGVP
                     EVHLQRLVFDAQGYGLARAESSLFEGTQAEERVAIEAAQAIGAELGVTLDASGATEPG
                     LSLKRQETEQPWSTCRRPWSLMYFTAHGRALPCCIAPFSVRGYGNYTLGDATQQTLRE
                     IWNGPAYRDFRASLLSDAPPAPCQNCGLRWSL"
BASE COUNT          736 a         1568 c         1461 g          727 t
ORIGIN      
        1 tgaccggcgc cgcggcgcag tcgctgctgg cgcatctgat cgagttgggg gaaatgggcc
       61 aagcccggga agcaaacggg gcgtggcatc gcgcggactg atcccctatc ggctttcccc
      121 ggctcggcgc caggcataga atcgattcag cgcaactcga ttcggggagt tacagatggt
      181 ccgccattct ctcgccattg cctcgctgct gctggcgctt tccgccggca cggcctctgc
      241 cggcatcatc agcgcgccgg cgctgcccgc cggcagcagc tatctgacaa ccgttgcgac
      301 cgccacccag atgacggtcg cccatgccta tgcccatctg cgcgagaagc cgacgaccaa
      361 atccgctttg ctggccacgt tgaagcatgg caccaaggtc gaggtgctgg acaaggtcga
      421 cgacggcaaa tggacgcatg tgaaagtcgg cagcaaggaa ggctatatct ccaccagcct
      481 gctgaaatag gtctcggcgc caggcggatg ggcctcagcc ggcattgccg ggcgaggcct
      541 ccttccgcgc tggagcatcc atgaacgaag ccagcccccg gggtcgcagc cagcggcagc
      601 gccccgccta tgaagccgtc gccccataag ccgtcccccc aggggccgtc tcactggcgg
      661 atggcgctgg tgctgggatg gctgacggcc ttcgggccgc tggccatcga cacctatctg
      721 ccgagctttc ccgccatctc ccgggatctc ggcgtcgagg cctccagcgt cgaggcgacc
      781 gtcgcggtct atttcctcgg gctcgccctc ggccagagct tctacgggcc catggcggac
      841 cgctggggcc gcaggccgcc gctctatgcc gggctcctgc tcttcatcct cgcctcggcc
      901 ggctgcgccc tgacccagag catcggcgcg ctgattgcgc tgcgcttcct ccaggcgctg
      961 ggcggcagcg cccagatcgt ggtcgcccgg gccgtggtgc gggaccattt cgacgagcgc
     1021 gaatcggccc gggtgctctc gcttctgatg ctggtcatgg gcgtggcccc catattggcg
     1081 ccgttgctgg gcggccagct cgtgggattc ttcggttggc gctcgatctt ctggaccctg
     1141 gccgctttcg ccaccgcctg cctggtcgcc gtctccttca ccttgccgga gagcctgccg
     1201 cccgagcggc gcctgcgcca aagcctggcg accgtgttcc ggctctacgg caacctcctc
     1261 gccgatcgcc aattcatggc gccggtcttg gccggctccc tgatcagcgc cggcatgttc
     1321 gcctatatcg ccggctcgcc cttcgtcttc atggagatct atggcgtccc gccggcgcat
     1381 ttcggcctgt tcttcggcag caacgccctg gggctgatcg ctgcctccca gatcaatgcc
     1441 cggctggtcc gccgctacgc cccggcccgg atcctgacga tcgtgctcat cgtcaccgct
     1501 tgcgccgggc tggtcctgct gggaaccgcc gcgacgggca tcggcggctt tcccggcctg
     1561 ttggtgccgc tgtttctctt cgtcgccagc ctgggcttcg tggcgcccaa tgcgacggcc
     1621 cttgccatgg ggccccaggg ccgcaacgcc gggagcgcct ccgccctgct cggggtgacg
     1681 cagttcctct gcggcggcat ctcgggcggg ctggtcagcg cgctttatga cgggacgccg
     1741 gtgcccatgg tgctggtgat cgcgctctgc ggactcggtg cctgcggaat ccatctgctc
     1801 ttcggccggc ggcggtagag cccaacaaga catttcgctg aggcaggggg aggtgcgacg
     1861 tgccgcggga catgggcggc gacggatcgc accatgccgg ccatggcgcg tctcaagctt
     1921 tccctcattc tcaagtccgg tgcgcgcatc gggcccggca aggcggcact cctggaaagc
     1981 gtgcgcgata cgggatcgat ctcggccgca gcgggaagcc ttggcgaggc accgacctcg
     2041 tatgagaagg cagagtggga tgtcgccggt ggaccccgcg ttctcaggag gcagcagcga
     2101 ctcgccgacg gcgaaccaga tatccgctca gcgcctgcag cgctgcgccg accccgacac
     2161 ggccaaggcc aatccgcacc cggtctcccg gcgtctccgc cagcgtcgac tgccagagac
     2221 ccgacggaag caacagaacg ccagcttcac gggcgagctc gaccgcaaac cggtcggcac
     2281 catcggaacc caggtagcgt ggacagacaa acgccgagtt ggcggacgcc tgcccctcga
     2341 aggcttcgag atggcgatcg aggaacagct ccacaagctc gcggttcgat cccagaatcg
     2401 ccttgtttcg ttcgacgata cgattttctg cccctagggc aatatgggtc agcacttcac
     2461 tgggcgcggc gagacagctc gatagcatgc tcttgaccag ctggacctcg gcaagaaacc
     2521 gtcggtcttg gcaggcaacc caacccacgc gcagcccagg cagcccgaac cccttcgaca
     2581 ccgcgttgat cgagacgccg cgctcgtaga tgtcggctag ccgaggcgag cgaagatggg
     2641 gatccaagtc aaccaagccg tacacctcgt cgttcaccag ccagaggcca tgtcgtcggc
     2701 agagtgcgac gagggccgcc agcgcggcgg gatcgatcga cgcgccggtc gggctgttgg
     2761 ggaagttcat caggacaagc cgagtgttgc cttgcagagc ggaagcgacc cgatcgacgt
     2821 ccagcaacca ggcgccgcgg tcttccagag gtacgccggt cgctgcgcaa atcgacgtga
     2881 cggcacgctc tgagggctga tagatcggaa cgaccaccac cgcgtggtca tcgggcgcga
     2941 gcaacgcccg cattgtgctc gtcaaaacct cctgggcgcc ggcacaacag acgatatggg
     3001 tatcgtcgag tttatcatat cgctccgcga tggcggagcg gagccaactt gcgccgcgcg
     3061 gatcggtgta gccgagcccg agttcctgcc aacgccgcct gtcctccggc cccgcaagtt
     3121 cgagcagggc ggagagcgtt agcgtcgcgg cctcggaacc ggacagatca tggcgaaccc
     3181 gcggactcca acgcgccagg aagccaccga gggcaaatgt ttcactcatg gtcggaatgt
     3241 tcacctgccg gcaacgcgaa ggaacgtcga gatgatctgg ccaccggcac gcagcgtgcc
     3301 ccgcagggag ccggcgacct tggacgcgcc gcccgcgcgg cagcggtagg gcagcggcac
     3361 ttcgaggatc cgcagaccgg cgcgcgccgc ccgcatctgc atctcgatgt tccagccata
     3421 gctcatctcg cgcatcccga gccgctgcag tacggcgtga tcgatggcgc ggaacgcgca
     3481 catgtcgctg tagcgcatgc cgtagagtgc accgatgccg aggccggcga cccggcccgc
     3541 caggacctga tgccagagca tggcaccggg ctcccgttgg ccgcgggtcc gggacgccag
     3601 cacgaagtca tggctgcccg ccagcacggg gccggcgatg cgcgcgatca gatcgccacg
     3661 gtcggcgccg tccccatcca ggaatgcgat cacccgacaa gccgggtcgg ccgcagcggc
     3721 gccggcggcg caggcgcgtc cgtatccgcg ccccgcatcg atcacccggg caccggcctc
     3781 ccgcgcggca tctcgcgtgg cgtcgacgct cccgccgtcg gctacgatga tgtcggccga
     3841 aaagtcacgg ggaatgccgc gaatgaccgc gccgatcgtc gcggcctcgt tcaacgtggg
     3901 aatcacgatg gctacaggct ccatcgcagc ccgcaattct gacatggcgc cggcggggcg
     3961 tcggagagca gcgacgcacg aaaatcgcga taggccggcc cgttccagat ctcccgcagc
     4021 gtctgctggg tcgcgtcgcc gagcgtgtag ttgccatatc cgcgcacaga gaacggtgca
     4081 atgcagcatg gcagggcgcg accgtgggcg gtgaaataca tcagcgacca tggacgacga
     4141 caggtcgacc aaggctgttc cgtctcctgc cgcttcaggc tcagccccgg ctctgtcgcc
     4201 cccgaggcat ccagcgtgac acccaattcg gcgccgatgg cctgggcagc ctcgatcgcc
     4261 acccgctctt ccgcctgggt gccctcgaac agcgagctct cggcacgggc taggccatag
     4321 ccttgcgcat cgaagacgag ccgctggaga tggacctccg gcacccccat gattgccgcc
     4381 aagcgcacga atgccggcag ctggtcgatg gtctctttca acccagtcaa ccagagagag
     4441 acgcggggtg tcgtggcccc gacgtcccgc tggtaggcga cgaacttccc ca
//
LOCUS       DATNBV010000211         2065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_432379,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000211 DATNBV010000000
VERSION     DATNBV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432379"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..428)
                     /locus_tag="VN627_05150"
     tRNA            complement(352..428)
                     /locus_tag="VN627_05150"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(392..394),aa:Met,seq:cat)
     gene            complement(467..1042)
                     /locus_tag="VN627_05155"
     CDS             complement(467..1042)
                     /locus_tag="VN627_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HXD02678.1"
                     /translation="MSGGADGSVAERGGRPFIVTAELPAGVLAWADGLRRAHFPPERN
                     HLAAHVTLFHAFAPSLREELRPLLARLAGEIGPIPARVAGLMNLGRGTAIALESPGML
                     ALRARIADHFHGALTAQDQHPPRLHITIQNKVTAPEARALQQALEGLVPERRFTFAGL
                     GLHRYTGPGWKPVGCWSFRGREVVDQPARRG"
     gene            complement(1085..1621)
                     /locus_tag="VN627_05160"
     CDS             complement(1085..1621)
                     /locus_tag="VN627_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXD02679.1"
                     /translation="MHPQQIPEVTLKTRIRDESIGGDNPFRWQDLKVRDEFAGKRVVV
                     FSLPGAFTPTCSNEQCPAFERLHDEFQKVGIDEVYCIAVNDAFVMYQWGLKLGVNKVR
                     LLPDGSGSFTRRMGMLINKDHLGFGMRSWRYAMVVDDNTVTHWFEEPGINDVGADEDP
                     YGQTAPEHILAALQDPLP"
     gene            complement(1866..>2065)
                     /locus_tag="VN627_05165"
     CDS             complement(1866..>2065)
                     /locus_tag="VN627_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXD02680.1"
                     /translation="MIGLPEGPITNPGRASIEAVLNPAKTDALYMVADGTGGHAFAAT
                     LEEHNRNVAKWFAIRRQRGEL"
BASE COUNT          351 a          684 c          683 g          347 t
ORIGIN      
        1 tcccaacacg caccgacgaa tgcttggaac ggcgatgttc acggctgatg tcataggcat
       61 gggggaagcg tatcctcagc ggctccttag gacaacgcct gaacgtccgc ttcgttgtcg
      121 ctagcggacg gtccgcttgg agggcttcct tgcccccagc ctctgtttcg gccgcatgcc
      181 agccgttcaa ttcgagccaa cactttgtcg cctgtatgtt gcacggagtt gccggcgatc
      241 ggctggaagc ggcgaaaagc gccggaaatc agcgcaacac gatggcaaca tgacaaggcg
      301 ccagccggac gcgctaactt tgaaaaacta tcgtaaattc aagggtttga atggtagcgg
      361 aggagggact cgaacccccg acacgcggat tatgattccg ctgctctaac cggctgagct
      421 actccgcccc gttcgggccc cggcgactgc cgggcaggcg gcgctcctag cctcgccggg
      481 cgggctggtc aaccacctct ctgccccgaa atgaccagca cccgaccggc ttccacccgg
      541 gcccggtgta gcggtgcagg ccgagccccg caaaggtgaa gcggcgctcg ggcacgagcc
      601 cctcgagcgc ctgctgcagg gccctggctt cgggcgcagt gaccttgttc tggatggtga
      661 tgtgcagccg cggcggatgc tggtcctgcg cggtcagggc gccgtggaaa tggtcggcga
      721 tccgcgcccg gagcgccagc atgcccgggc tctccagcgc gatggcggtt ccccgcccaa
      781 ggttcatcag ccccgctacc cgtgcgggga tcggcccgat ctcacccgcg agcctcgcca
      841 gcaagggccg cagttcttcc cgcagggacg gcgcgaaggc gtggaacagg gtgacatggg
      901 cggcaaggtg gttgcgctcc ggggggaaat gggcgcgccg caggccgtca gcccaggcca
      961 gcaccccggc gggcagttcg gccgtgacga tgaacggccg ccctccccgc tcggcgacgc
     1021 tgccgtccgc gccgccgctc atcggccgcc ccgctcagga caggccgatg tccgccgaac
     1081 caagctacgg cagcgggtcc tgcagggccg cgaggatgtg ctcgggcgca gtctggccat
     1141 aggggtcttc atccgcgccg acgtcattga tcccgggctc ctcgaaccag tgggtaacgg
     1201 tgttgtcgtc caccaccatg gcatagcgcc acgagcgcat tccgaaaccc aggtgatcct
     1261 tgttgatcaa catgcccatg cgccgggtga aactgcccga tccgtccggg agaaggcgca
     1321 ccttgttcac ccccagcttc aggccccact ggtacatgac gaaggcatcg ttgacggcaa
     1381 tgcagtaaac ctcgtcgatg ccgaccttct ggaactcgtc atgcagccgc tcgaaggccg
     1441 ggcactgttc gttcgagcag gtcggcgtaa aggcccccgg cagcgagaag acgaccaccc
     1501 gcttgccggc gaactcgtcg cgaaccttca agtcctgcca gcggaaggga ttgtcgccgc
     1561 cgatcgattc gtcgcggatg cgcgtcttca gggtaacctc gggaatttgc tgggggtgca
     1621 tggctctctc gcgaaaatag cggcggacag ggcgcaatgg ccggcacgtg aaaaacccgg
     1681 cggacccctg caggttccac ggcagaggca gggggaggcc cgaagaaccg gcagcgccag
     1741 atcagctagt cgttcgccgt tttcagactg atcgcgcgca agggcatcgg gaccgcggtg
     1801 ttcccgctga tcgtcgcctt gatcgccgtg gccgatctgg tctgggcacg ccgctcgctg
     1861 cctgcctaga gctcacccct ctggcggcga atggcgaacc acttcgccac attgcggttg
     1921 tgctcttcca gtgtggcggc aaaggcgtgg ccgccggtgc catcggccac catgtagagc
     1981 gcgtcggtct tcgccgggtt gagcacggcc tcgatcgagg cgcggcccgg gttggtgatc
     2041 gggccttcgg gcaggccgat catcg
//
LOCUS       DATNBV010000212         5844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2345947,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000212 DATNBV010000000
VERSION     DATNBV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345947"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1325)
                     /gene="carA"
                     /locus_tag="VN627_05170"
     CDS             complement(159..1325)
                     /gene="carA"
                     /locus_tag="VN627_05170"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HXD02681.1"
                     /translation="MADAASSHVSQPKGATGVLVLADGTVVWGRGFGASGEAVGEVCF
                     NTAMTGYQEVMTDPSYAGQIVTFTFPHIGNVGCNEEDLESLVDGAMGCVVREDVTEPS
                     NFRSHERFGDWLKAKGKIGVAGIDTRALTRRIRLSGAPNAVIAHHPDDKFDIPALIKR
                     AKDWPGLEGMDLARVVSRSGQEGWEGGVWTLGKGYARSPHTPRPHVVAIDYGAKDNIF
                     RNLVKAGAQVTVVPAQTPLDAILALKPAGVFLSNGPGDPAATGAYAVPIIQGLLEKDV
                     PIFGICLGHQMLGLAAGARTTKMHQGHRGANHPVLRKADGVVEITSMNHGFAVDNTAL
                     PDTVEETHVSLFDGSNCGIAVKGKKAFGVQYHPEASPGPQDSFYLFEKFVGMLG"
     gene            1485..1937
                     /locus_tag="VN627_05175"
     CDS             1485..1937
                     /locus_tag="VN627_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HXD02682.1"
                     /translation="MIRDTIKAAQVTAMKAGDKTRLAAVRLILAKLKDKDIELRMASS
                     VPDDDTVVVDVLQKMAKQRRESITLYEQGGRQELADVEKGELAVIEEFMPAQLSDAEV
                     SAAIEAIKAEIGASGMKDMGKVVAALKARHGSQLDMGKASGLVKAALA"
     gene            2059..3939
                     /gene="dnaG"
                     /locus_tag="VN627_05180"
     CDS             2059..3939
                     /gene="dnaG"
                     /locus_tag="VN627_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HXD02683.1"
                     /translation="MLTPQWLDELRSRITLSSVIGRTTRLTKAGHEFKACCPFHNEKS
                     PSFTVNDEKGFYHCFGCGAHGDAIRWMTDQRGLSFMDAVKELAAEAGMEVPAPDPRAA
                     KAAEQRDGLHDVMAAAQEWFVAQLAAPEGERARAYLATRGFDPHTLTRFGFGYAPEGR
                     QALKGALSRFPEDKLIEAGLRIAVEDKEPYDRFRGRLMLPIEDARGRVIAFGGRILDK
                     TKTDAPKYLNSPDTPLFDKGRTLYNLHRAGPASRQSGRLIVVEGYMDVIALAAAGFAE
                     AVAPLGTALTEHQLGLLWRLVETPILCFDGDAAGQRAAMRAITRALPLLKPAHSLRIV
                     RLPTGLDPDDLIRQQGAGAMEDLLGQARPLVDMLWEHECDALPLATPEDKAGLKARLM
                     GHVELITDQEIRGLYKRELSDRFSAWAYPRREGGGGSPQGWNPAPGRSPLGRPAPARR
                     PGPWREAAPPPPSPETAQRLKRATSGANEALAAAVLAGLCIYPEAIMPNAELLARAGP
                     SLARHHRLLDALLDAADSAEFQGQSLEKESLTSTLTRHGVSPPPPDEYARIRFPFTSP
                     TAQPAEALGALETAIRWLVLVPELDAGIAAATERFDVEEQGRLRSSKAELERRLKEQM
                     RG"
     gene            4099..>5844
                     /gene="rpoD"
                     /locus_tag="VN627_05185"
     CDS             4099..>5844
                     /gene="rpoD"
                     /locus_tag="VN627_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HXD02684.1"
                     /translation="MMEEEISGNEREGSDAPLIDLNEASIKKLIARAKRRGVITYDEL
                     NEALPQDQMSSEQIEDIMAAISEMGVNIVESDEDAVDPEEQRAEDQEDADAVDERPDL
                     IKKKETVERTDDPVRMYLREMGAVELLSREGEIAIAKRIEAGRDTMILGLCESPITFH
                     AIIQWSEALNAGEMQLREILDLDAMLSKAPENLPDEDSVEEPEISEATAGPSFKEEEE
                     PEEEETVDEDDEDGIERRARRPAEEEEEDNTLSLAQMEAALKPAALERFAEITDLFRR
                     FEKIQAERLDVLGAGGTFAEAKEREYQQLREDLTAQVESVQFHATKIEYLVDNLYAFN
                     RRLTTLGGQMLRLAERHKVNRKDFLDAYVGRELDEGWLQQHAGRDKKWAAFADNEADA
                     VERIRAEVADIAAATGMSLTEFRRIVNMVQKGEREARIAKKEMVEANLRLVISIAKKY
                     TNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRI
                     PVHMIETINKLVRTSRQMLHEIGREPTPEELAEKLAMPLEKVRKVLKIAKEPISLETP
                     IGDEEDSHLGDFIEDR"
BASE COUNT         1059 a         1866 c         2039 g          880 t
ORIGIN      
        1 gcattggctc ggaaccagag ctgagaagcc ctccctctca aggaggaggg ttgggtgggg
       61 gtgcacacag gcttcggtgg gacgcaaggc tgagccttgc tccccctccc ccaacccctc
      121 cccgacggtg aggggggcag aaatggcaga tgccactctc aacccagcat ccccacgaac
      181 ttctcgaaca ggtagaagct gtcctgcggg ccggggctcg cctcggggtg gtactgcacc
      241 ccgaacgcct tcttcccctt gaccgcgatg ccgcagttgg agccgtcgaa cagggagacg
      301 tgggtttcct cgaccgtgtc cggcagggcg gtgttgtcga ccgcgaagcc gtggttcatc
      361 gaggtgatct cgaccacgcc gtcggccttg cgcagcaccg ggtggttcgc gccgcggtgg
      421 ccctggtgca tcttggtggt gcgggcaccg gcggcgaggc cgagcatctg gtggccgagg
      481 cagatgccga atatcggcac gtccttttcc agcaggccct ggatgatcgg cacggcatag
      541 gcgccggtcg ccgccgggtc gccagggccg ttcgacagga acacgccggc gggcttgagc
      601 gcgaggatcg cgtccagcgg ggtctgcgcg gggacgacgg tgacctgcgc gccggccttc
      661 acgaggttcc tgaagatgtt gtccttcgcg ccgtagtcaa tcgcgacaac atgcgggcgc
      721 ggggtgtgcg ggctgcgggc atagcccttg cccagcgtcc acacgccgcc ttcccaacct
      781 tcctggccgc tccgcgaaac gacgcgggcg aggtccatgc cttctaggcc gggccagtcc
      841 ttcgcgcgct tgatcagggc gggaatgtcg aacttgtcat cggggtggtg ggcgatcacg
      901 gcgttgggcg cgccggagag gcggatgcgg cgggtgagcg cgcgggtgtc gatccccgca
      961 acgccgatct tgcccttggc cttgagccaa tcgccgaacc tttcgtgcga gcggaagttg
     1021 ctcggctcgg tcacgtcctc gcgcaccacg cagcccatcg cgccgtcgac cagggattcg
     1081 agatcttcct cgttgcagcc gacgttgccg atgtggggga acgtgaaggt gacgatctgg
     1141 ccggcgtagg aaggatcggt catcacctcc tggtacccgg tcatcgccgt gttgaagcag
     1201 acttcgccca cggcctcacc gctggcgccg aagccgcggc cccagacgac ggtgccatcg
     1261 gcgagaacga ggactccggt tgcccctttc ggttgtgaca cgtgcgaaga ggcggcgtcg
     1321 gccatatttg tggcgctccg taagggggtt tccggcgata ggtgctaagg ggcggcggct
     1381 agaccccaag ccccttcgcg tcaacctagg aatttgggaa aaccccggct aattggaaca
     1441 gcatgggccg caggccgcgg caatttcaca acgacaggaa tattatgatc cgcgacacga
     1501 tcaaggccgc gcaagtgacc gccatgaagg ccggcgacaa gacccggctc gccgccgtcc
     1561 gcctcatcct cgccaagctg aaggacaagg acattgaatt gcgcatggcc agctccgtgc
     1621 cggatgacga tacggttgtg gtcgacgtgc tgcagaagat ggccaagcag cggcgcgaat
     1681 cgatcacgct ttatgagcag ggcgggcggc aggagctggc cgacgtggaa aagggcgagc
     1741 tggcggtgat cgaggagttc atgccggcgc agctttccga tgccgaggtg tcggcggcga
     1801 ttgaggcgat caaggcggag atcggggcca gcgggatgaa ggacatgggc aaggtcgtgg
     1861 cggcgctgaa agcgcggcat gggagccagt tggacatggg caaggccagt gggctggtga
     1921 aggcggcttt ggcgtgacta ggcccctccc gcaggcggga ggggtttggg gtgggcctgc
     1981 ggttgtgtgc gcaggcttgc ccacccccag ccccttccgc ctgcgggagg ggattaagag
     2041 cgctgcaagg gtggtgtcgt gctgacccct caatggctgg acgaactgcg cagccgcatc
     2101 acgctgtcca gcgtgatcgg ccgcaccacg cggctgacca aggcggggca cgagttcaag
     2161 gcctgctgcc cgttccataa cgaaaagtcg cccagcttca ccgtcaacga cgagaagggt
     2221 ttctaccact gcttcggctg cggggcgcat ggcgatgcga tccggtggat gaccgaccag
     2281 cgcgggctgt cattcatgga tgcggtgaag gaactggcgg ccgaagcggg gatggaggtc
     2341 cctgcccccg atccgcgcgc ggcgaaggcg gcggagcagc gcgatgggct gcatgacgtc
     2401 atggccgcgg cgcaggagtg gttcgtggcg cagctggctg cgcccgaggg cgagcgggcg
     2461 cgggcctacc tcgcgacgcg gggcttcgat ccgcatacgc tgacgcggtt cggcttcggc
     2521 tatgcgccgg agggacggca ggcgctgaag ggtgccctgt cacgctttcc cgaggacaag
     2581 ctgatcgagg cgggcctgcg gatcgccgtc gaggacaagg aaccgtacga ccggttccgc
     2641 gggcggctga tgctgccgat cgaggatgcg cgcgggcggg tgatcgcctt tggcgggcgg
     2701 atcctcgaca agaccaagac cgatgcgccc aagtacctga attcgcccga tacgccgctg
     2761 ttcgacaagg ggcgcacgct ttacaacctg caccgcgccg ggcccgcctc gcgccagtcg
     2821 gggcggctga tcgtggtcga gggctacatg gacgtgattg ccctggccgc cgccggtttc
     2881 gccgaagccg tggcaccgct gggcacggcg ctgaccgagc accagctggg gctgctgtgg
     2941 cggctggtgg aaacgccgat cctgtgtttc gacggggacg cggcgggtca gcgggcggcg
     3001 atgcgggcga ttacccgcgc cctgcccctg ctcaagcccg cccatagcct gcggatcgtg
     3061 cgcctgccga cggggctcga tcccgacgac ctgatcaggc agcagggcgc gggcgcgatg
     3121 gaggatctgc tgggtcaggc ccgaccgctg gtcgacatgc tgtgggagca tgagtgcgat
     3181 gccctgccgc tggcgacgcc ggaggacaag gcggggctca aggcgcggct catgggccat
     3241 gtcgagctga tcacggatca ggagattcgc gggctctaca agcgtgagct gtcggaccgg
     3301 ttttcggcct gggcctatcc gcgccgcgag ggcggcggcg ggtcgccgca ggggtggaac
     3361 ccggcgccag gccgctcacc cttgggccgc cctgccccgg cccgccggcc gggcccctgg
     3421 cgcgaggccg caccgccccc gccctcgccc gaaacggcac agcggctgaa gcgggctact
     3481 tcgggagcca acgaggcgct ggcggctgcc gttctcgccg ggctgtgcat ctatcccgaa
     3541 gcgatcatgc ccaacgccga actgctcgcc cgcgccggcc cttcgcttgc ccgtcaccac
     3601 cggctgttgg atgcgctgct cgatgcggcg gacagcgccg aattccaggg ccaatccctt
     3661 gaaaaggaaa gcctcacctc cacattaact cggcacggcg tgagcccccc gcccccggac
     3721 gaatatgccc gcatccgctt tcccttcacc agccccacgg cgcagcccgc cgaggcgctg
     3781 ggcgcattgg aaacggcaat ccgctggctc gtcctggtgc cggagctcga tgccggaatc
     3841 gctgcggcaa cggagcggtt cgacgtagag gagcagggtc ggctgcggtc cagcaaggca
     3901 gagctggagc ggcggctcaa ggaacagatg cgcggctgac ctggttggca ggcgaacgac
     3961 atgaataggg caaaaggaag gctggcattt cgccttcggt tcgggcaatg gcccgcatcg
     4021 gggtataatc cagcatcctg acagaatcgg acctgcgaag ccggccaacg aggcggagtc
     4081 gcgagagcac acggcggtat gatggaagaa gaaatttccg gcaatgagcg cgagggcagc
     4141 gacgccccgc tcatcgatct caacgaagcc tcgatcaaga agctgatcgc ccgcgccaag
     4201 cgccggggcg tgatcaccta tgacgagctc aacgaggctc tgccgcagga ccagatgtct
     4261 tccgagcaga tcgaggacat catggccgcg atctcggaga tgggcgtcaa catcgtcgag
     4321 agcgacgagg acgcggtcga tccggaggag cagcgcgccg aggatcagga agacgcagat
     4381 gccgtcgacg agcggccgga cctgatcaag aagaaggaaa cggtcgagcg cacggacgat
     4441 cccgtgcgca tgtacctgcg cgagatgggc gcggtcgaac tgctcagccg cgagggcgaa
     4501 atcgccatcg ccaagcgcat cgaggccggt cgcgatacca tgatcctggg cctgtgcgaa
     4561 agcccgatca ccttccacgc catcatccag tggtccgagg cgctgaacgc cggcgagatg
     4621 cagctgcgcg agatcctcga tctcgacgcc atgctctcga aggcccccga gaacctgccg
     4681 gacgaggatt ccgtcgagga gccggaaatc agcgaggcca ccgccggccc ctccttcaag
     4741 gaagaggagg agcctgagga agaggaaacc gtcgacgagg acgacgagga cggcatcgag
     4801 cgccgcgccc gccgccccgc cgaggaagag gaagaggata acaccctctc cctcgcccag
     4861 atggaagcgg cgctgaagcc ggctgccctc gaacgcttcg ccgagatcac cgacctgttc
     4921 cgccggttcg agaagatcca ggccgagcgg ctcgacgtgc tgggcgccgg cggcactttc
     4981 gccgaggcca aggagcgcga ataccagcag ctgcgcgagg atctcaccgc gcaggtggag
     5041 agcgtccagt tccacgcgac caagatcgaa tacctggtcg acaacctcta tgccttcaac
     5101 cggcggctga ccacgctggg cgggcagatg ctgcgcctgg ccgaacgcca caaggtcaat
     5161 cgcaaggact tcctcgacgc ttacgtcggc cgcgagctcg acgagggctg gctccagcag
     5221 cacgccgggc gcgacaagaa gtgggcggcc ttcgccgaca acgaagccga cgcggtcgag
     5281 cgcatccgtg ccgaggtggc cgatatcgcc gccgccaccg gcatgtcgct caccgagttc
     5341 cgccggatcg tcaacatggt ccagaagggc gagcgcgagg cgcgcatcgc caagaaggaa
     5401 atggtcgagg ccaacctgcg cctcgtgatc tcgatcgcca agaagtacac gaaccgcggc
     5461 ctgcagttcc tggatctcat ccaggagggc aacatcggcc tgatgaaggc ggtggacaag
     5521 ttcgagtacc gccgcggcta taaattctcc acctatgcca cctggtggat ccggcaggcg
     5581 atcacccgct cgatcgccga ccaggcccgc accatccgca tccccgtgca tatgatcgag
     5641 acgatcaaca agctggtgcg cacctcgcgc cagatgctgc atgagatcgg ccgcgagccg
     5701 acgccggagg aattggccga gaagctcgcc atgccgctgg agaaggtgcg caaggttctc
     5761 aagatcgcca aggagccgat ctctctcgag acgcccatcg gcgacgagga ggacagccac
     5821 ctcggcgact tcatcgagga ccgc
//
LOCUS       DATNBV010000213         3946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2347374,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000213 DATNBV010000000
VERSION     DATNBV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2347374"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="VN627_05190"
     CDS             <1..179
                     /locus_tag="VN627_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HXD02685.1"
                     /translation="IGAGLRIEVTCECDPCSRMEELAPGLKAALTPDWRGGVLGRVIS
                     DGEIAVGDEVRIEQ"
     gene            176..1372
                     /locus_tag="VN627_05195"
     CDS             176..1372
                     /locus_tag="VN627_05195"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXD02686.1"
                     /translation="MTFKKLDDLGDVTGKVALVRVDLNLPMQDGAVTDDTRVRAAAPT
                     ILELSAMGAKVLLLAHFGRPKGERVSTMSLSMVVDCLQQVLGKEVMFVPEIAGPVVAQ
                     SVGILRPGDIAVLENTRFWKGEEKNDPELVKAIAENGDFYVNDAFSAAHRAHASTEGL
                     AHVLPAYAGRSMQAELEALEKALGKPVKPVAAVVGGAKVSSKLDVLKHLVSKVDHLII
                     GGGMANTFLAARGVDVGKSLCEHDLKATAEEILDNAEKAGCTVHLPYDVVVAKEFQAN
                     PPSLRTCNVHEVAADEMILDVGPQAVEALGDALKTCRTLVWNGPLGAFEMAPFDAATV
                     ALARIAAALTKEGSLVSVAGGGDTVAALNHAGVAADFSYISTAGGAFLEWMEGKELPG
                     VVALEG"
     gene            complement(1392..2717)
                     /locus_tag="VN627_05200"
     CDS             complement(1392..2717)
                     /locus_tag="VN627_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HXD02687.1"
                     /translation="MRELLRNARLRWDRATAHPPSDSDPWAWCAGIAAAFLGLLLLRL
                     DVPDRIYFDEVHYVKAARVLLSLAKPMNPEHPLVGKELIAGGILLFGDTPWAWRIMPA
                     LFGTIGLYAFARALWLASLRRFATIAGTLLLATSFTWFVMSRIAMLDIFMASFTMLAM
                     WMLAGAVRRPDQGRWRLMFAGLFLGLALGSKWNAVAAAALPGLTFLAMRLRQHGRNFM
                     LVTEGAPVPGISLAEAGLWLGTLPLLVYFATFLPAFFYATKAIDPLRIVDYQGYMLRL
                     QHSVIKHHPYQSVWWQWVLDARSIWFLYDNVDGAQRGIVMLGNPFAMAAGLPALGWAL
                     WAGIRRRRMDALAAAVLYLACVGMWVANGKPIQFYYHYLLPSAFLMACLALALDELWR
                     RADRWRWLAPASVALSAAMFATFWPILSAAKLCCGKPSFNWWMWWKGWR"
     gene            2796..>3946
                     /locus_tag="VN627_05205"
     CDS             2796..>3946
                     /locus_tag="VN627_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HXD02688.1"
                     /translation="MKKTTGQDRSITRNWRPATQAVRGGTWRSEHGETSEALYLTSGY
                     TYDDAATVAARFAGEQEGMTYSRLQNPTVAMLEERIALLEGAEACRAQASGMAAMTAA
                     LLCQLSAGDHIVAGKAAFGSCRWLVDSLLPRFGVETTVIDGTVNAEWEAAIRPNTKVF
                     FYETPANPTLELVDMTFVNGLARSHGITTVVDNAFCSPVIQRPLDFGADVVAYSATKF
                     MDGQGRVLAGAVCGSAQFINDKLLPFHRNTGPNLAPFNAWVVLKGLETLELRLRQQSA
                     NALEVGRFIEGRVPKVMHPGLESHPQFALAQQQMKLSGAIFSFELAGREQAWAVLDAL
                     QLIDISNNIGDTRSLMCHPATTTHHNMGPEGRAAMGVSDGMLRLNVGLE"
BASE COUNT          709 a         1260 c         1353 g          624 t
ORIGIN      
        1 ccattggcgc ggggctgcga atcgaagtga cctgcgagtg cgatccgtgc agccgcatgg
       61 aagaactggc tcccgggctt aaggccgcgc tcacgccgga ctggcggggt ggcgtgctgg
      121 gccgggtaat cagcgacggc gagatcgccg tgggcgacga agtgaggatc gagcaatgac
      181 cttcaagaag ctggacgact tgggcgacgt gaccggcaag gtcgcgctgg tgcgcgtgga
      241 cctcaacctg ccgatgcagg atggggccgt gaccgacgat acccgcgtgc gcgctgccgc
      301 gccgaccatc ctcgagcttt cggccatggg cgcaaaggtt ctgctgctgg cccactttgg
      361 ccggcccaag ggcgagcggg tctcgaccat gtcgctttcg atggtggtcg attgcctcca
      421 gcaggtgctg ggcaaggaag tgatgttcgt gcccgaaatc gccggcccgg tggtcgcaca
      481 gtccgtcggc atcctgcgcc ctggcgacat tgccgtgctg gagaacaccc ggttctggaa
      541 gggcgaggag aagaacgatc ccgagctggt gaaggcgatc gccgagaacg gcgatttcta
      601 tgtgaacgac gccttctccg ccgcgcaccg cgcccatgcc tccaccgagg gccttgccca
      661 cgtgctcccc gcctatgcgg gccggtccat gcaggccgaa ctggaagcgc tcgagaaggc
      721 gctcggcaag ccggtcaagc cggtcgccgc cgtggtgggc ggggccaagg tttcttccaa
      781 gctcgatgtg ctcaagcacc ttgtttccaa ggttgatcac ctgatcatcg gcggcggcat
      841 ggccaacacg ttcctcgccg cgcgcggggt cgatgtcggc aagagcctgt gcgagcatga
      901 cctgaaggcg actgccgaag agattctcga caatgccgaa aaggccggtt gcaccgtcca
      961 cctgccgtac gacgtcgtgg tggcgaaaga attccaggcc aatccgccaa gcttgcggac
     1021 ctgcaacgtc catgaggtcg ctgcggacga gatgatcctc gatgtcggcc cgcaggcggt
     1081 ggaagcgctc ggcgacgccc tcaagacttg ccgcacgctg gtgtggaacg ggcccttggg
     1141 cgccttcgag atggcgccgt tcgacgccgc cacggtcgcg ctggcgcgga tcgccgcggc
     1201 gttgaccaag gaaggctcgc tggtctccgt tgcgggcggc ggtgataccg tggccgcgct
     1261 gaaccatgcc ggtgtggctg cagacttcag ctacatctcg actgcgggcg gcgccttcct
     1321 ggaatggatg gaaggcaagg aactgcccgg cgtggtcgcg ctggaagggt aagcgcgggg
     1381 accagcgggt cttaccgcca gcccttccac cacatccacc agttgaagct aggtttgccg
     1441 cagcacagct tggccgccga gaggatcggc cagaaggtgg cgaacatcgc cgccgacaat
     1501 gccacgctgg ccggcgcgag ccagcgccag cggtccgccc gccgccacag ctcgtccagc
     1561 gcgagggcga ggcacgccat cagaaaggcg ctgggtagca ggtagtggta gtagaactgg
     1621 atcggcttgc cgttggccac ccacatgccg acgcaggcaa ggtagagcac ggcggcggcc
     1681 agcgcgtcca tgcggcggcg cctgatcccc gcccatagcg cccagccgag tgccggcagt
     1741 cccgccgcca tggcgaacgg attgccgagc atgacgatac cgcgctgcgc tccgtcgacg
     1801 ttgtcgtaaa ggaaccagat cgagcgggca tcgagcaccc attgccacca gaccgactgg
     1861 taggggtggt gcttgatcac cgagtgctgc aaccgcaaca tgtagccttg gtaatcgacg
     1921 atcctgagcg gatcgattgc tttcgtcgcg tagaagaagg ctggcaggaa cgtggcgaaa
     1981 tagaccagca gcggcagggt gccgagccac aggcccgctt cggccaggct gatcccgggg
     2041 acgggggcgc cttcggttac cagcatgaaa ttgcgcccgt gctgcctcag gcgcatggcg
     2101 aggaaggtga gacccggcaa agcggcagcg gctacggcgt tccactttga gcccagcgcc
     2161 agaccgagga acagcccggc gaacatcaat cgccagcggc cctgatccgg gcgtctcacc
     2221 gccccggcca gcatccacat ggccagcatg gtgaagctgg ccatgaagat atcgagcatg
     2281 gcgatgcggc tcattacgaa ccaggtgaag ctggtggcga gcagcagggt gccggcgatc
     2341 gtggcaaagc ggcgcaggct ggccagccac agggcgcggg caaaggcata gaggccgatg
     2401 gtgccgaaca gggcgggcat gatccgccag gcccacggcg tgtcgccgaa cagcaggatt
     2461 cccccggcga tcagttcctt gccgaccagc gggtgctccg ggttcatcgg cttggccagc
     2521 gagagcagca cgcgggccgc cttcacgtag tgcacctcgt cgaaatagat tcgatcgggc
     2581 acgtcgaggc gcagcagaag taggccaagg aaggccgcgg cgatgcccgc gcaccaggcc
     2641 cacggatcgc tgtcgcttgg cggatgggcg gtggcccggt cccagcgcag ccgggcgttg
     2701 cggagaagct ccctcatggc aggcgcgata gccgcactgg tccgggtact gcaaccgcct
     2761 tgccccgcgc cccgcgcgct tctaatgcgg ccgccatgaa aaagaccacc ggacaggacc
     2821 gctcgatcac ccgcaactgg cgcccggcga cgcaggccgt tcggggcggc acgtggcgat
     2881 ccgaacatgg cgagacgagc gaggcgctct acctcacctc tggatatacc tacgacgatg
     2941 ccgcaacggt agccgcgcgg ttcgcgggcg agcaggaggg aatgacctat tcccgcctgc
     3001 agaatcccac tgtggccatg ctggaagagc gcatcgccct gctcgaaggt gccgaggctt
     3061 gccgcgctca ggcgtcgggc atggccgcga tgaccgcggc cctgctgtgt cagctgtccg
     3121 ccggcgatca cattgttgcc gggaaggccg ctttcggttc gtgccgctgg ctggtcgaca
     3181 gcctgctgcc gcgcttcggc gtcgagacca cggtcatcga cgggactgtg aacgccgagt
     3241 gggaggctgc gattcggccc aacaccaagg tgttcttcta cgagaccccc gctaatccca
     3301 cgcttgagct ggtcgacatg accttcgtga acggcctggc tcgcagccac ggcataacca
     3361 ccgtggtcga caatgccttc tgctcgccgg tgatccagcg gccgctcgat ttcggtgccg
     3421 acgtggttgc ctattcggcc accaagttca tggacgggca gggccgggtc ctggcgggcg
     3481 cggtctgcgg gtcggcgcaa ttcatcaatg acaagctgct gccgtttcac cgcaataccg
     3541 ggccgaacct cgcgccgttc aatgcctggg tggtgctcaa gggcctggag acgctggagc
     3601 tgcgtctgcg ccagcagagc gcgaatgcgc tcgaagttgg gcggttcatc gaaggccgcg
     3661 tgcctaaagt catgcatcca ggcttggaga gccaccctca gttcgccctc gcccagcagc
     3721 agatgaagct ctcgggcgcg atcttctcgt tcgagctggc gggccgcgaa caggcatggg
     3781 ccgtgctcga tgcgttgcag ctgatcgaca tctcgaacaa cattggcgac acccgctcgc
     3841 tgatgtgcca ccccgcaacg acgacccacc acaacatggg gccggaaggc cgcgcggcca
     3901 tgggggtgag cgacggcatg ctgcggctga atgtcgggct ggaaga
//
LOCUS       DATNBV010000214         2759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_772684,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000214 DATNBV010000000
VERSION     DATNBV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772684"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1426
                     /gene="tilS"
                     /locus_tag="VN627_05210"
     CDS             128..1426
                     /gene="tilS"
                     /locus_tag="VN627_05210"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXD02689.1"
                     /translation="MPEIDIAKLMAPLGPFERPPSIAVAVSGGSDSLGLGLLLAEWVR
                     GQGGRLHVLSVDHGLRPEAAGECARVGEIFAAIPGCSAHVLRWHGEKPARGIQAAARA
                     ARYALLTDWCRAQGVLHLAIAHTAEDQAETVAMRRAHGSGAAGLAGMPALRAEQGVRL
                     LRPVLSVSRPAIRAWLQARGQSWIEDPSNDLTKFERVRVRRSLGAKEAGTLLDQAQRL
                     GEMRDGLERAAARLLAEAGQVHEAGYVSLALAPLSEAAPDLQATALRQVILAVSGGDY
                     APDLDDLLSSLATPRTLGGCFVQSLQGRLNVFREAAAIDAPAPVAPGWAGSWDNRFSL
                     AVAGDLSPTTGWAIGPLGERGLRQAVDRFGIRLKRHPIPLPARLALPALWHGEHLAAQ
                     PHLDLGQGLAARAAPRHTVTTCGFTVAAGRPHTIYSSVPC"
     gene            1512..>2759
                     /gene="ftsH"
                     /locus_tag="VN627_05215"
     CDS             1512..>2759
                     /gene="ftsH"
                     /locus_tag="VN627_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXD02690.1"
                     /translation="MNFGRNLWIWAAVIVLLFVLFEMFQGGANPGNASELSYSEFLHK
                     VQAGEVNNVTIKGPEITFQTGNSTYTTYAPDDPKLIETLTAKGVAIKAGPQEEKNLLF
                     IALVQWAPIILIVAFWIFMLRQMQSNGGKAMGFGKSRARLLTERQGRVTFDDVAGIEE
                     AKTELEEIVDFLKDPQKFQRLGGKIPKGCLLVGPPGTGKTLLARAIAGEANVPFFTIS
                     GSDFVEMFVGVGASRVRDMFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTL
                     NQLLVEMDGFEANEGVILIAATNRPDVLDPALLRPGRFDRQVVVPNPDVAGREKILKV
                     HIRNVPLAPNVDLKVIARGTPGFSGADLANLVNEAALLAARAGRRVVGMAEFEAARDK
                     VMMGAERRSLVMTEKEKELTAYHE"
BASE COUNT          446 a          918 c          941 g          454 t
ORIGIN      
        1 tgcgttgccc tgcgccagct tgagaagcgc tatcccgacg cctcggcgaa cgtgaaggac
       61 cgcgccgtcc gcgccaagaa gaactacagc tgcacctgag gcgccccgcc ggctgcgcgc
      121 aagccccatg ccggagatcg acatcgcaaa gctgatggcg ccgctcggtc cgttcgagcg
      181 gccgccttcg atcgcggtgg cggtgtcggg cgggtcggat tccctcggcc ttgggctgct
      241 gctggcggag tgggtgagag ggcagggcgg tcgcctccat gtgctcagcg tcgatcacgg
      301 gctgcgcccg gaagcggccg gcgaatgcgc gcgcgttggc gaaatctttg ccgcaattcc
      361 aggctgttcg gcgcacgtct tgcgatggca tggcgagaag ccggcgcgcg ggattcaggc
      421 ggcggcgcgg gcggcacgct atgccttgct gacggactgg tgccgcgcgc agggcgtgct
      481 gcacttggcc atcgcccaca cggcagaaga ccaggcggag accgtggcga tgcgccgggc
      541 ccatggcagc ggcgccgccg gcctcgccgg catgccggca ttgcgggccg agcagggcgt
      601 acgcctgctg cgccccgtgc tctcggtctc gcgcccggcg atccgggctt ggcttcaggc
      661 gcgcggacaa agctggatcg aggatccgag caacgatctc acgaagttcg agcgtgtgcg
      721 ggtccggcgg agcctcggcg ccaaggaggc ggggaccctg ctcgaccagg cacagcgatt
      781 gggcgagatg cgcgatgggt tggagcgcgc cgccgcgcgc ctgctggccg aagcgggaca
      841 ggtgcacgag gcgggctatg tctcgctcgc cctcgcgccg ctctcggagg ccgcgccaga
      901 cttgcaggcc accgccctgc gccaggtgat ccttgccgtg agcggcggcg attatgcccc
      961 tgatcttgat gatcttcttt cttcgctcgc caccccgcgg accttagggg ggtgcttcgt
     1021 gcagagcctg caaggccgcc tgaatgtgtt ccgggaggcc gccgcgattg atgcgccagc
     1081 ccccgtcgcc cccggctggg ccggttcctg ggataaccgg ttcagcctgg cggtcgccgg
     1141 cgacctgtcc cccacgacgg gctgggccat cggcccgctg ggcgagcggg gcctgcgcca
     1201 ggccgtggat aggttcggaa tccgcctgaa gcgccatccc atccccctgc cggcccgtct
     1261 ggccctgccg gccctgtggc acggggaaca cttggccgct caaccgcatt tggacctggg
     1321 acaagggctt gcggccaggg ccgcacctcg ccataccgtg acaacatgcg gctttacagt
     1381 tgcggccggg cggccgcaca ctatctattc atcagtgccg tgctagtttg gaatgattgc
     1441 aaatcggggg ataagtcttc ccgctacgcg cccaaatcgg ccggctttat ccagttaatg
     1501 aggatcgaac ggtgaatttc ggcaggaatc tgtggatttg ggcggcggtc atcgtgctgc
     1561 tgttcgtgct gttcgagatg ttccagggtg gggccaaccc gggcaatgcc tcggaactct
     1621 cctactcgga gttcctccac aaggttcagg cgggtgaggt caacaacgtc accatcaagg
     1681 ggcctgagat caccttccag accggcaaca gcacctacac gacctatgcg ccggatgatc
     1741 cgaagctgat cgaaaccctg accgccaagg gcgtcgccat caaggccggc ccgcaggaag
     1801 agaagaacct gctgttcatc gccctggtgc agtgggcgcc gatcatcctg atcgtggcgt
     1861 tctggatctt catgctgcgc cagatgcagt ccaacggcgg caaggccatg ggcttcggca
     1921 agtcgcgtgc gcgcctgctg accgagcgcc agggccgcgt cacgttcgac gacgtcgccg
     1981 gcatcgagga agccaagacc gagctcgagg agatcgtcga tttcctcaag gacccgcaga
     2041 aattccagcg cctgggcggc aagatcccga aaggctgctt gctggtcggc cctccgggca
     2101 ccggtaagac gctgctggcg cgcgccatcg cgggcgaagc caacgtgccg ttcttcacca
     2161 tctccggctc cgacttcgtc gagatgttcg tcggcgtcgg cgccagccgc gtgcgcgaca
     2221 tgttcgagca gggcaagaag aacgcgccct gcatcatctt catcgacgaa atcgacgcgg
     2281 tcggccgtca tcgcggcgcc ggcctcggcg gcggcaatga cgagcgcgaa cagaccctca
     2341 accagttgct ggtcgagatg gacggcttcg aagcgaacga aggcgtgatc ctgatcgcag
     2401 ccaccaaccg ccccgacgtg ctcgacccgg ccctgctgcg tcccggccgc ttcgaccgcc
     2461 aggtcgtggt gccgaacccc gacgtcgccg gccgcgagaa gatcctgaag gtgcacatcc
     2521 gcaacgtgcc gctggcgccg aatgtcgacc tcaaggtcat cgcgcgcggc acgccgggct
     2581 tttcgggcgc ggatctcgcc aatctggtca acgaagctgc tctgctggcc gcccgcgccg
     2641 gccgccgcgt cgtcggcatg gccgagttcg aggccgcgcg cgacaaggtg atgatgggcg
     2701 ccgaacgccg ctcgctcgtg atgaccgaga aggaaaagga gctgactgcc tatcatgag
//
LOCUS       DATNBV010000215         7670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1397844,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000215 DATNBV010000000
VERSION     DATNBV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397844"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VN627_05220"
     CDS             complement(<1..900)
                     /locus_tag="VN627_05220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXD02691.1"
                     /translation="MLRSLHRGIANPSSPARRRSWVTVFNRHRDDYQVPVALEEAGLL
                     AFHFGEFYASPRSQRWLPGPLRRLNALSHPALARDRVRTAPASFVLQMLGIALRVPMR
                     LVFPVSDLLLALRGAAVARRAGADLLCYGNYVPPRWAIAPGTRVVLFEYHPLAAYTFD
                     ILKADVAAHPQVSESFRKEQRELARGAVQTAWQRADRVICASSMTRRSLVHAGCDPGR
                     IVVIPYGCRPPARPVQVRPDGPCRFLFVGQGIQRKGLHHLLLAWAQLAPAEAELTVVC
                     YHVDPGIARMAADRGVHILPHQPR"
     gene            complement(967..2352)
                     /locus_tag="VN627_05225"
     CDS             complement(967..2352)
                     /locus_tag="VN627_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HXD02692.1"
                     /translation="MEAASDTAIVPPPAAAPEKWGHPRGLWTLNGTELWDRISFHGMV
                     AMLVLYMTGDLLKPGRMEHILGFGAYRHFLESVFGAVTPAAIATQTFGLYYAVLTCLP
                     LVGGWLGDKLVSRRFAVTTGALTMTAGHFAMAFDATFLVALALLVCGAGLLRGNLKPQ
                     IRALYHAGDRRLADAFQLYSMVVNGGAFIAPLASGAVAKYYGWHAGFAVAGFGMLIGL
                     VWYLAGSRYLPPDPPKGVHVHRAPLTRHQKRDVLGLFAMWPVSLGFWTAQAQIWNVYN
                     LWLRDHVQMKVGGFDVPVPWLQALDGLAPAAYVPVVILLWRWQARRGTEPDLYTKMGV
                     GCVIFALSTLLLAGAPFLAGADGLAPLWIPVLFHVASNWGGAYFSPVMETFYAARAPE
                     QWRGSLLGIDALSVSASSILSGTMGSWYETVSPPVFWLATAAIAAAAGVYVLALRGVM
                     RRWLGDEAEQP"
     gene            2442..2642
                     /locus_tag="VN627_05230"
     CDS             2442..2642
                     /locus_tag="VN627_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HXD02693.1"
                     /translation="MYQTPEDRPIYRLLTGKDDRAFCERVSEALAQGWRLYGSPSLAW
                     DTENNCMKAAQAVVWHEADVVK"
     gene            complement(2647..3534)
                     /locus_tag="VN627_05235"
     CDS             complement(2647..3534)
                     /locus_tag="VN627_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02694.1"
                     /translation="MPDDFASDTALLPPPPRRGGGTILLALAVAFLLGAAGVAYVAWQ
                     GLIPLARPAPEAEPAPAAPDAGQAPHPSAPAQASIAALAAGQQDQLDARVAALSARLD
                     ALTIQAQAAAGNAGRAEGLLVAFAARRALDRGAPLNTLEDQLRLRFGDSQPHAVATVI
                     AAAREPVTLDQLVAGLDSLAPSLTRAPVEASAWSRIRQELSGLFVIRHEAAPSPSPQR
                     QLDHARLLLESGRTDDAIDEVRRLPGAGGASDWFVAARRYDDARRALDVLETAALIGN
                     PAQKSAAAASTPPAAAPAV"
     gene            complement(3551..4267)
                     /locus_tag="VN627_05240"
     CDS             complement(3551..4267)
                     /locus_tag="VN627_05240"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HXD02695.1"
                     /translation="MNEAAGLPLIVVRPEPGCGATLAAARELGLDARAFPLFEVGPVA
                     WTAPAAAQFDVLLAGSANVFRHGGTQLAALRGLPVHAVGATTSEAARGAGFAVAATGA
                     GGLQPVLDALPAGTRVLRLAGAERVALTLPPGVSMSERTVYAARALPLPPDLAALLTA
                     PAVIALHSAEAARHLAAELERAGLARGAIALVTIGPRVTAAAGPDWAAVVTAERADDA
                     ALLAKARDLCQTPGKTGRGP"
     gene            complement(4264..5196)
                     /gene="hemC"
                     /locus_tag="VN627_05245"
     CDS             complement(4264..5196)
                     /gene="hemC"
                     /locus_tag="VN627_05245"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HXD02696.1"
                     /translation="MIAEPVKPLRLGTRRSPLAMAQAEEARDRLCAAHGWSEAAVELV
                     PVTASGDRIQDRPLAEIGGKALWTKELDAWLLAGEIDFAVHSAKDVETLRPLGIAIAA
                     VLPREDVRDVLVGATTIHALPAGARIGTSAPRRAAQLLHARPDCTVVPFRGNVQTRLA
                     KLAAGEADATLLAMAGLNRLGQHGVGHPLAVEDWLPAPGQGAIAIECRSGDEPTRSLL
                     AAIDDGPSHAALLAERALLAALGGNCHSPVAVLTRSEGDRLAMSAALFSPDGAERTAL
                     ELVFPASDATGPARLAAALLDAAPEAIRAHFSPA"
     gene            5241..6293
                     /gene="tsaD"
                     /locus_tag="VN627_05250"
     CDS             5241..6293
                     /gene="tsaD"
                     /locus_tag="VN627_05250"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXD02697.1"
                     /translation="MTLVLGIESSCDETAAALVRLGPGAAREIVAQRIASQDDAHRPY
                     GGVVPEIAARAHVERVTPLVEATLADAGLGLADVDAIAATAGPGLIGGVMVGLVTAKA
                     LAMAGDKPLIAINHLEGHALSPRLADPALAFPYLLLLASGGHCQLLRVDGVGRYTRLA
                     TTIDDALGEAFDKTAKILGLGYPGGPAVERLATTGNPKAVPLPRPLLGSADPNFSFAG
                     LKSAVLRAKQAGSHADADIAAAFQQAAIDCVIDRSRRALAAAGAVSALVVAGGVAANQ
                     AIRAALESLAAEAALRFVAPPLALCTDNAAMIAWAGLERLTSGDFAPASLDLAARPRW
                     PLDPDAAPVRGAGVKA"
     gene            6290..7285
                     /locus_tag="VN627_05255"
     CDS             6290..7285
                     /locus_tag="VN627_05255"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HXD02698.1"
                     /translation="MSAIGIIGAGAWGTALAQAMASDGSEVLLWAREPQLVAEINDRR
                     TNSLFLPGASLAQSIRATGDLAELGALATLLVVVPAQFLASVLGNLPGGSRDLVLCAK
                     GIEAGTGRLMADVARDAAPAATLAVLSGPTFAHEVAAGLPTAVTLACAGGEAQWARLA
                     PAISRPMLRPYYSDDVTGAEIGGAVKNVLAIACGVVDGLKLGQNARAALIARGYAEML
                     RFGVARGAKAETLAGLCGLGDLVLTCSSTSSRNFSLGKALGEGLSAAEALAGKLSVAE
                     GAATAPVLADLAEREGIPMPIVAAVNRLLKGEASAGAVVAELLARPLRAEQGGPA"
     gene            7282..>7670
                     /locus_tag="VN627_05260"
     CDS             7282..>7670
                     /locus_tag="VN627_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HXD02699.1"
                     /translation="MTTPGAATENNEDIAALAKGGRTNFFGFLLRLVARIPFLFIASR
                     LYGAAAMGRFASALVMVELAGMLATLGQKRGLAARLAEEREHPANVVADALLLSTMFS
                     VLAMIALYIFPEAMYPKGNFSHLDELLA"
BASE COUNT         1118 a         2783 c         2748 g         1021 t
ORIGIN      
        1 gcgcggctga tgcgggagga tgtggactcc gcgatccgct gccatccggg cgatgcccgg
       61 atcgacatgg tagcacacca cggttagctc cgcttcggcg ggcgcaagtt gcgcccaggc
      121 cagtagcagg tggtgcaggc ccttgcgctg gatgccttgg ccgacgaaca ggaaccggca
      181 gggcccatcg ggacgcacct gcaccggccg ggccggcggg cggcaaccat aggggatgac
      241 cacgatccgt ccgggatcgc agccggcatg gaccagcgag cgccgggtca tggaactggc
      301 gcagatcacc cggtcggcgc gctgccaggc ggtctggacc gcgccgcggg cgagctcgcg
      361 ctgctccttg cgaaaggatt cgctcacctg cgggtgggcg gccacatcgg ccttgaggat
      421 gtcgaaggtg taggccgcca gcgggtgata ttcgaacagg accactcggg tgccaggcgc
      481 gatggcccag cgcggcggca cgtagttgcc gtagcacagg agatcggcgc cggcccgccg
      541 cgcgaccgcg gccccgcgca aggcgaggag gagatcgctg accgggaaga ccagacgcat
      601 cggcacacgc agggcaatgc ccagcatctg gaggacgaac gaggccgggg cggtcctgac
      661 ccgatcgcgc gcgagggcgg gatgggacag ggcattcagc cgccggagcg ggccgggcag
      721 ccagcgctgg ctgcgggggg aggcgtagaa ttcgccgaag tggaaggcga gcaggccggc
      781 ctcctccagc gcgacgggga cctggtaatc gtctcggtgg cggttgaaca cggtcaccca
      841 gctacgccgc cgcgcgggcg aggaggggtt tgcgatcccg cgatgaaggg aacggagcat
      901 ctccgtttgg gatatccgca tcgccgcgcg aattcaatcc gcgcgcgcgt gccgggtgtc
      961 ggcgggtcag ggctgctccg cctcatcccc cagccaccgc cgcatcaccc cgcgcagggc
     1021 cagtacatag acccccgccg ctgccgcaat ggcggcagtc gccagccaga acaccggcgg
     1081 cgagaccgtc tcgtaccacg aacccattgt cccgctgagg atggaggacg ccgagaccga
     1141 cagcgcgtcg atcccaagca gggagccacg ccattgttcc ggcgcgcggg ctgcgtagaa
     1201 ggtttccatc accggcgaga agtaggcccc gccccagttt gaggcgacgt ggaacagcac
     1261 cgggatccac agcggggcaa ggccgtccgc cccggcgagg aacggcgcgc cggccagcag
     1321 cagggtggag agggcgaaga tcacgcagcc cacgcccatc ttggtataaa ggtcgggctc
     1381 ggtcccccgc cgcgcctgcc agcgccacag caggataacc acgggcacat aggccgccgg
     1441 cgcgaggcca tcgagcgcct ggagccacgg caccggcacg tcgaagccgc cgaccttcat
     1501 ctgcacgtga tcgcgcagcc acaggttgta gacgttccag atctgcgcct gcgccgtcca
     1561 gaaccccagg ctcaccggcc acatcgcgaa cagccccagc acgtcgcgct tctggtgccg
     1621 ggtcagcggg gcgcggtgga cgtggacgcc cttgggcgga tcggggggga ggtagcgcga
     1681 gcccgccaga taccagacca gcccgatcag catgccgaac ccggccacgg caaagcccgc
     1741 atgccagccg tagtatttgg ccacggcccc gctcgccagc ggagcgatga aggcgccgcc
     1801 gttgaccacc atcgaataga gctggaaggc atcggccagc cgccggtcgc ccgcatgata
     1861 gagcgcccgg atctgcggct tgagattgcc ccgcagcagc cccgcgccgc agaccagcag
     1921 ggcaagcgcg acgaggaacg tggcgtcgaa ggccatggcg aagtgcccgg cggtcatcgt
     1981 cagcgcgccg gtcgtcaccg cgaaccggcg cgagacgagc ttgtcgccca gccacccacc
     2041 caccagcggc aggcaggtca gcacggcata gtagagcccg aaagtctgcg tcgcgatcgc
     2101 cgccggggtc acggcgccga acacgctttc gaggaaatgg cggtaggcgc cgaacccgag
     2161 gatatgctcc atccgccccg gcttcagcag gtctccggtc atgtagagca ccagcatggc
     2221 gaccatgccg tggaagctga tccgatccca cagctcggtc ccgttcagcg tccacaggcc
     2281 ccgcgggtgg ccccatttct cgggagcggc ggcgggcggg gggacgatcg cggtgtcgct
     2341 ggcggcttcc atgcgggtcc tttgcgggtg agcggggatg ggcgttatgt gccgggtggg
     2401 agggcttggc acaagcggcc aatcggctct aacatccgct catgtaccag acccccgaag
     2461 accgcccgat ctatcgcctc ctcaccggca aggacgaccg cgccttctgc gagcgggtgt
     2521 cggaagccct ggcccagggc tggcggctct acggttcgcc cagcctcgcc tgggataccg
     2581 agaacaactg catgaaggcc gcccaggccg tcgtctggca cgaggccgac gtggtgaagt
     2641 gaccggtcag accgcgggtg cggcggcagg cggggtagag gccgcagcgg ccgatttctg
     2701 tgccggattg ccgatcagcg ccgccgtttc cagaacgtcg agggcgcggc gggcatcgtc
     2761 atagcggcgc gccgccacga accagtcgct cgcgccgccc gcgcccggca ggcggcggac
     2821 ctcgtcgatg gcatcgtcgg tccgcccgga ttcgagcagc aggcgggcat ggtcgagctg
     2881 gcgctggggc gacgggctcg gcgccgcctc gtggcggatc acgaacaggc ccgacagttc
     2941 ctggcgaatg cgcgaccagg cgctggcttc caccggcgcg cgggtcaggc tgggcgcgag
     3001 actgtcaagc ccggcgacca gctggtcaag cgtcaccggc tcgcgggcgg ccgcgatcac
     3061 ggtcgccacg gcatgcggct ggctgtcgcc gaagcgcagg cgcagctggt cttcaagggt
     3121 gttgagcggc gccccgcggt caagcgcccg ccgtgcggcg aaggcgacga gcaggccctc
     3181 ggcccgcccg gcattaccgg cggcggcctg ggcctggatc gtgagcgcat cgagccgggc
     3241 cgagagcgcc gccacgcggg cgtcgagctg gtcctgctgg ccggcggcaa gcgcagcgat
     3301 gctggcctgc gccggggccg agggatgggg cgcttgcccg gcgtccggcg cggccggggc
     3361 cggctcggcc tcgggcgcgg ggcgggccaa ggggatcagc ccctgccagg caacataggc
     3421 cacgcccgcc gctcccagca ggaaggccac ggccagcgca agcaggatcg tgccgccacc
     3481 gcgcctgggc ggcgggggca gcagggcggt gtcgctggca aaatcgtcag gcattgcgcg
     3541 cggtgatcct tcagggtcct cggccggtct ttcccggggt ttggcacaag tcgcgcgcct
     3601 tcgccagcag ggcggcatcg tccgcccgct ctgcggtgac aacggcagcc cagtccggtc
     3661 cggccgccgc ggtgacccgc ggaccgatgg tgacgagcgc aatcgcgccc cgcgccagcc
     3721 cggcccgttc cagctccgcg gcaaggtggc gggccgcctc ggcggaatgg agggcgatca
     3781 ccgccggcgc ggtgagcagc gcggcaagat ccggcggcag ggggagggcg cgcgccgcat
     3841 agaccgtccg ctcgctcatg ctcacccccg gcggcagggt aagcgccacc cgctcggccc
     3901 cggccagccg cagcacgcgg gttccggcgg gcagggcatc gagcaccggc tgaagcccgc
     3961 ccgcgccggt cgccgccacg gcaaaccccg cgccgcgcgc cgcctcggac gtggtcgccc
     4021 ccacggcatg gacgggcagg ccgcgcagtg ccgccagctg ggtccccccg tggcgaaaca
     4081 cattggcgct gcccgccagc agcacgtcga actgcgcggc cgccggtgcg gtccaggcca
     4141 ccggccccac ctcgaacagc ggaaaggccc gggcatcaag ccccagttcg cgggccgcgg
     4201 ccagcgtcgc accgcagccc ggctcgggcc gcaccacgat cagcggcagg ccggccgctt
     4261 cgttcatgcc ggggagaagt gcgcccggat cgcctcgggc gcggcgtcca gcagagcggc
     4321 ggcgaggcgc gcggggccgg tcgcatcgct ggccgggaag accagctcca gcgccgtccg
     4381 ttccgcccca tccgggctga acagggccgc gctcatggcc aggcggtcgc cctccgagcg
     4441 ggtcagcacg gccaccgggc tgtggcaatt gccgcccagc gccgccagca gggcccgctc
     4501 cgccagcagg gccgcgtggc tagggccgtc gtcaatggct gcgagcaggc tgcgcgtggg
     4561 ctcgtcgccg ctgcggcact cgatggcgat tgccccctgg cccggggcag ggagccaatc
     4621 ctctaccgcc aggggatggc caacgccatg ctgccccagc cggttgagcc cggccatcgc
     4681 cagcagcgtc gcatcggcct cgcctgccgc gagcttggcc agccgcgtct ggacgttgcc
     4741 gcgaaacggg acgaccgtgc agtccggccg ggcatggagc aattgcgcgg cccggcgcgg
     4801 cgcgctcgtc ccgatccgcg cgccggcggg cagggcatgg atcgtcgtcg cgccgaccag
     4861 cacgtcgcgc acgtcctcgc gcgggagcac ggcggcgatg gcgattccca gggggcgcag
     4921 cgtctccacg tccttggccg aatggaccgc gaagtcgatc tcgccggcga gcagccaggc
     4981 gtcgagctcc ttggtccaca gcgccttgcc gccgatctcg gccagcggac ggtcctggat
     5041 ccggtcgcca ctggcggtca ccggcaccag ctccaccgcc gcctcgctcc agccgtgggc
     5101 ggcgcacagt cggtcacggg cctcctcggc ctgggccatg gcaagggggg agcggcgggt
     5161 gccgagacgg agcggcttta caggttcagc aatcatggcg cggcttgtgc taggcggctt
     5221 tgtcgctaga ggaaagcggg gtgacgctcg ttctcggcat agaatcctcc tgcgatgaaa
     5281 ccgcagccgc gctggtccgc ctcgggcccg gtgccgcgcg cgagatcgtc gcccagcgga
     5341 tcgcctcgca ggacgatgcg caccggccct atggcggggt cgtccccgaa atcgccgcgc
     5401 gcgctcatgt cgagcgggtc accccgctgg tcgaggcgac cctggccgat gccggccttg
     5461 gtcttgccga tgtcgatgcc atcgccgcca cggccgggcc ggggctgatc ggcggggtca
     5521 tggtcggcct ggtcaccgcc aaggcgctgg ccatggcggg ggacaagccg ctgatcgcaa
     5581 tcaaccacct cgaaggccac gcgctctccc cgcgcctggc cgatccggcg ctggcattcc
     5641 catatctgct gctgctcgcc tcgggcggtc attgccagct gctccgggtc gatggcgtcg
     5701 gccgctacac gcgccttgcg accacgattg acgatgcgct cggcgaggct ttcgacaaga
     5761 ccgccaagat cctcggcctc ggctatcccg gcggtccggc ggttgaacgg ctggccacca
     5821 ccggcaatcc caaagccgtc cccctgccgc gcccgctcct cggcagcgcc gatcccaatt
     5881 tctccttcgc cggtctgaag agcgcggtcc tgcgcgccaa gcaggcgggc agccacgccg
     5941 atgccgatat cgccgccgcc ttccagcaag ccgcgatcga ctgcgtgatc gaccgcagcc
     6001 ggcgcgccct tgctgctgcg ggggcggtga gcgcgctcgt cgtcgccggc ggggtcgccg
     6061 ccaaccaggc gatccgcgcc gcgctggaaa gcctcgccgc cgaggccgcc ctgcgcttcg
     6121 ttgccccgcc gctggcgctg tgcaccgaca atgcggcgat gatcgcctgg gcggggcttg
     6181 agcggctcac gtccggcgat ttcgcccccg ccagccttga tctcgccgcc cgtccgcgct
     6241 ggccgctcga tcccgatgcg gctccggtgc gcggggcagg ggtcaaggca tgagcgccat
     6301 aggcatcatc ggggcagggg cctggggcac ggcgctggcc caggccatgg cgagcgacgg
     6361 cagcgaggtc ctgctctggg cgcgcgagcc gcagctcgtc gccgaaatca atgaccggcg
     6421 caccaacagc ctgttcctcc ccggcgcctc tctggcgcaa tcgatccgcg ccaccggcga
     6481 tctcgccgaa ctcggcgccc ttgctaccct gctggtggtc gtcccggccc agttcctcgc
     6541 ctcggtgctc ggcaacctgc ccggcggcag ccgcgatctc gtgctctgcg ccaaggggat
     6601 cgaggcgggc accggccgtc tcatggccga tgtcgcgcgc gacgccgcgc cggcggccac
     6661 cctcgcggtg ctctccgggc cgaccttcgc ccacgaagtc gccgccggtc tgccgacagc
     6721 cgtcaccctc gcctgcgccg gcggggaagc gcagtgggcc cgcctcgccc cggcgatctc
     6781 gcggccgatg ctgcggccct attattccga tgatgtgacc ggggccgaga ttggcggcgc
     6841 ggtcaagaac gtcctcgcca ttgcctgcgg cgtggttgat ggcctcaagc tcggccagaa
     6901 cgcccgcgct gccctgattg cccgcggcta tgccgaaatg ctgcgctttg gcgtggcacg
     6961 cggcgccaag gcggaaacct tggccggcct gtgcgggctc ggcgatctgg tgctgacctg
     7021 ctcgtccacc tccagccgca acttctcgct cggcaaggcg ctgggcgagg gtctcagcgc
     7081 ggccgaggcg ctcgccggca agctcagcgt ggccgaaggc gcggccacgg ccccggtcct
     7141 ggccgacctt gccgagcgcg aaggcatccc catgccgatc gtcgcggccg tcaaccgcct
     7201 gctcaagggc gaggccagcg cgggcgcggt cgtcgccgag ctgctcgccc gtccgctccg
     7261 cgccgaacag ggcggcccgg cgtgaccacc cccggggctg ccacggaaaa caacgaggat
     7321 atcgccgccc tggccaaggg cgggcggacc aatttcttcg gcttcctgct gcgcctcgtc
     7381 gcgcgcatcc cgttcctgtt catcgccagc cgcctctatg gcgcggcggc gatgggccgc
     7441 ttcgcctcgg cgctggtcat ggtcgaactg gccggcatgc tggccacgct cggccagaaa
     7501 cgcggcctcg ccgcacgcct cgccgaagag cgtgagcatc cggccaatgt cgtcgccgac
     7561 gccctgctgc tgagcaccat gttctcggtc ctggcgatga tcgcgctcta catcttcccc
     7621 gaggcgatgt atcccaaggg caatttctcg cacctcgacg agctgctggc
//
LOCUS       DATNBV010000216         6181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4608757,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000216 DATNBV010000000
VERSION     DATNBV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4608757"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VN627_05265"
     CDS             complement(<1..205)
                     /locus_tag="VN627_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02700.1"
                     /translation="MKKTAVIAAALAAGFVHPGVAVAAPGLADEVYPATVEAGEPEVE
                     ARYGRLGGGPDSGEDELKLEASYG"
     gene            377..1348
                     /gene="trxB"
                     /locus_tag="VN627_05270"
     CDS             377..1348
                     /gene="trxB"
                     /locus_tag="VN627_05270"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HXD02701.1"
                     /translation="MATHTTRMLIIGSGPAGLTAAIYGARAGLQPIVVQGLQPGGQLT
                     ITTDVENYPGFRDVIQGPWLMQEMQAQAEHVGTRMIWDTIVEVDLSGPPFRAIADSGD
                     VYEGETLVIATGAQARWLGVPGEADLGGKGVSACATCDGFFYRGKKVVVIGGGNTAVE
                     EALYLTNHSPDVTVVHRRDSFRAEKILQDRLFAHPHISVAWNKAVKSFVGELPGGLTG
                     VEVVDTVTGETSIIPADGAFVAIGHAPATELFRGKLELDAAGYIVVEPGTPKTSIPGV
                     FACGDVMDHTYRQAVTAAGTGCMAALDAERFVAEREFAAAKAPKVTA"
     gene            complement(1349..2134)
                     /locus_tag="VN627_05275"
     CDS             complement(1349..2134)
                     /locus_tag="VN627_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 1, exosortase A system-associated"
                     /protein_id="HXD02702.1"
                     /translation="MIRRHLEFTCAGARLVGTMDTPTEGVCGRTGLLLVSGGNELRSG
                     AWGGQARLAARLAAAGYPVFRFDRRGVGDSMGENQGFRHSAEDIAAALAAFREAAPDL
                     RQVIGFGNCDAAAALMLGAGAGCDGLILANPWTIEEGEADAPSADRLRAHYRRRLLDP
                     AALRRLLRGGVRLGPLARSLLSALRPAAPASLADELRKGLAGFGGPVSILLAGRDRTA
                     QTFLARWDKADPRLRHCPSASHSFVEPEAQEWLAERLLEALGA"
     gene            complement(2131..2787)
                     /locus_tag="VN627_05280"
     CDS             complement(2131..2787)
                     /locus_tag="VN627_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02703.1"
                     /translation="MIASWSCPQPGGTSSEEYGLAFDRGRDRRLLIVPALFDEANRLR
                     RFTAETMRHLDTAGIDCFLPDLPGTNESIAPLARQTLETWRQAMAAAGRHFGATHVLA
                     LRGGALVASALPAFALAPVTGAAILRQMIRARIVASREVGLDERTESLLEEARTEGIT
                     LSGYQLSAGMAAGLEEAGPVEASTIPPGGPPLWLRAEPGEDPVQSAALADHVAAELAP
                     "
     gene            complement(2795..3058)
                     /locus_tag="VN627_05285"
     CDS             complement(2795..3058)
                     /locus_tag="VN627_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXD02704.1"
                     /translation="MTTELDRTLRQILADVLGLAPERVAAFDADTGLFGHLPELDSMS
                     VAGLLTEMEDRFGFIIDDDEVDGELLETYGALLAFAAAKQAAA"
     gene            complement(3094..4152)
                     /locus_tag="VN627_05290"
     CDS             complement(3094..4152)
                     /locus_tag="VN627_05290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXD02705.1"
                     /translation="MGWHRAYSRSNFAGVYVIAVVYLSDLRKASQDPSLAGLVRSARD
                     PFDRAEWWNLLARHCDITPRYVLARDESDGTALLPLMAGRGVIRPFANWYTFRWRPLV
                     SPGADAARLLAAAFRDLARNHWRIELRGIPGEDGTAEALLDALRAAGWQATISPHDVN
                     HVLPIGGRDYAAYLGGRPGHLRSTLKRKAGRTLCEVLTGFSTDAWATYEAIYAASWKG
                     EEGSPAFLRAFAEAEGAAGRLRLGIGRIDGTPVAAQMWTVEQGTAYIHKLAYREDAAA
                     HSPGTTLTAALMAHVIDVDRVELVDFGTGDDAYKRDWMELARPRYHLSAFQPGDPRSW
                     PHLARQALRRLAAARHDR"
     gene            4141..5667
                     /locus_tag="VN627_05295"
     CDS             4141..5667
                     /locus_tag="VN627_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HXD02706.1"
                     /translation="MPSHASPAIRPLDHLALAGRDEDPALLLRSGVLTYQDLRARIAA
                     LSGWLSHRLPEAGTRVATWAAKGELTCLMPLAAARAGLVHVPINPLLKRAQVTHILRD
                     SGSALLIGSPARLATLDADDIAPGCRTMGEDMALAEAAALGRFLPPSSADPDALAAIL
                     YTSGSTGRPKGVMLSHANMWLGAESVASYLQLSREDRTLAVLPLSFDYGQNQLLSTWY
                     AGGAVAPLDYLAPRDVVKAIARYDITTLAAVPPLWVQLAEQDWPPEVAGKLRRLTNSG
                     GALTVGLVRRLRALFPQARIFAMYGLTEAFRSTYLDPALIDSHPTSMGRAIPHAEILV
                     MADDERLAADGEEGELVHCGPLVAHGYWHDAERTAERFRPAPSISTYGGTAVWSGDRV
                     RRDDEGLLYFVGRRDAMIKSAGNRISPQEIEEAALATGLVAEAVAIGVPDPRLGQAVH
                     LVVRAAEETSDAETELPRKLMAELPNFMQPKVIHWRPALPVGPNGKIDRAGLAAELCE
                     "
     gene            5664..>6181
                     /locus_tag="VN627_05300"
     CDS             5664..>6181
                     /locus_tag="VN627_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HXD02707.1"
                     /translation="MKPLGPIPEGFRSKEGVLQVDSQDVRGLVAEAGGTPLFVYSRAM
                     IADRVARLRAALPERVALHYAIKANPFSPLLHVMSELVDGFDVASGGELQKLVELDIA
                     ASRISFAGPGKRDAELTAAIAAGVTINLESEGEAERALRLADEAGIRPRLAIRVNPDF
                     DLKGSGMKMGGGA"
BASE COUNT         1036 a         2067 c         2040 g         1038 t
ORIGIN      
        1 cgccgtaact ggcctcaagc ttcagctcat cttccccgct gtcgggcccg ccgccgagcc
       61 ggccgtagcg cgcctccacc tcgggctcac ccgcttcgac ggtggccggg tagacctcat
      121 cggccaggcc gggtgccgcg accgccacgc ccggatgaac gaaacccgcg gcaagtgcgg
      181 cggcgataac tgcggtcttc ttcatgaata tacccctcgg tctggcgtgg cccgttaggg
      241 ctagtgcaaa tcattcgcaa caacattttt gagggacatt tccttgatcc agatcaaacc
      301 gggcaaattc caccgagccg gactcgcatt ccgcctgcgc ggtggctatc tcgcggccaa
      361 ctgacggaag gaccatatgg cgacccatac cacccgcatg ctgatcatcg gttctggccc
      421 cgcaggcctc accgcagcga tctatggtgc ccgcgccggc ctgcagccga tcgtagtcca
      481 gggactgcag ccgggcgggc agctcacgat caccaccgac gtggagaact atccgggctt
      541 ccgcgacgtg atccagggcc cgtggctgat gcaggagatg caggcacagg ccgagcacgt
      601 cggcacgcgg atgatctggg acacgatcgt cgaggtagac ctctccggcc ccccgttccg
      661 ggccattgcc gattcgggtg acgtctacga gggcgagacc ctggtgatcg ccacgggcgc
      721 ccaggcgcgc tggctgggcg tgccgggcga ggcggatctc ggcggcaagg gcgtttcggc
      781 ctgcgccact tgcgacgggt ttttctatcg cggcaagaag gtcgtcgtga tcggtggcgg
      841 caacaccgcg gtcgaggagg cgctctatct taccaaccac tcgccggacg taaccgtggt
      901 tcaccgccgc gattccttcc gcgccgaaaa gatcctgcag gaccgcctgt tcgcccatcc
      961 tcacatctcg gttgcgtgga acaaggcggt gaaaagcttt gtcggcgaac tgccgggcgg
     1021 gctaacgggg gttgaagtcg tcgatactgt caccggggaa acgtcgatta ttcccgctga
     1081 cggtgccttc gtcgccattg gccacgcccc ggcgaccgaa ctgttccgcg gaaagctcga
     1141 actcgatgcc gccggttata tcgtggtcga accgggcacg ccgaaaacct cgattcccgg
     1201 cgttttcgcc tgcggtgacg tcatggacca cacctatcgc caggcggtga ctgccgccgg
     1261 gaccggctgc atggctgccc tggatgcgga gcggttcgtg gcggagcgtg aattcgccgc
     1321 ggccaaggcg cccaaggtca ccgcgtaatc aggcgccgag cgcctccagc aggcgctcgg
     1381 ccagccactc ctgcgcctcg ggttcgacga acgagtgcga ggccgagggg caatgcctga
     1441 ggcgaggatc ggccttgtcc caccgcgcga gaaaggtctg ggccgttcgg tcgcggccag
     1501 ccagcaatat cgagaccggg ccgccgaacc ctgctagtcc cttgcgcagt tcatcggcaa
     1561 gcgaggcggg ggcggcaggt cgcagggcgc tcagcaacga tcttgccagc gggccgaggc
     1621 gaacaccgcc ccgcagcagg cgccgcagcg ctgctggatc cagtagccgc cggcgataat
     1681 gggcccgcag gcggtcggcc gatggcgcat cagcctcgcc ctcctcgatc gtccatgggt
     1741 tggcgagaat gagcccatcg catccggcgc cggcgccgag catcagcgcc gcggccgcgt
     1801 cgcagttgcc aaaaccgatg acctgccgaa ggtccggcgc tgcctcgcgg aacgctgcca
     1861 gcgcggcggc gatgtcctcg gccgaatgcc gaaagccctg gttttccccc atgctatcgc
     1921 ccacgcctcg gcgatcgaac cggaacaccg gatagcccgc cgccgccagc cgcgccgcca
     1981 gcctggcctg tccgccccag gctcctgagc gcagctcgtt gccgccgctc accagcagca
     2041 gcccggttcg gccgcacacg ccttcagtcg gggtgtccat ggtcccgacc agccgagccc
     2101 cggcgcaggt gaattccagg tgacggcgga tcatggcgcc agttccgccg cgacatggtc
     2161 cgcgagcgcc gccgattgca ccgggtcctc tcccggctca gcccgcagcc acaatggagg
     2221 tccgcccggc gggatcgtgc ttgcctccac tggtccagcc tcctccaagc cagcggccat
     2281 tcctgcggat agctggtagc ccgacagcgt tatcccctcc gtccgcgcct cctccagcag
     2341 ggattcggtg cgctcgtcca gtccgacctc gcgggatgca acaatccgcg cccggatcat
     2401 ctgccgcagg atcgccgctc cggtgaccgg tgccagcgcg aaggcgggca gggcagaggc
     2461 gaccagtgcc ccgccgcgaa gggcgaggac gtgggtcgcg ccgaagtgcc ggcctgccgc
     2521 cgccatggct tgccgccagg tctccagcgt ctggcgggcc agcggggcga tgctctcatt
     2581 ggtgcctggc aggtcgggta ggaagcagtc gatccctgcg gtgtcgaggt gccgcatggt
     2641 ctcggcggtg aaccggcgca ggcgattggc ttcgtcgaac agggcgggga cgatcaggag
     2701 gcggcgatcc cgcccccgat cgaacgcaag accgtattcc tcgctgctgg tgccgccggg
     2761 ctggggacag gaccagctgg cgatcacgcg ggtctcaggc tgctgcctgc ttcgctgcgg
     2821 caaaggccag caacgcgccg tatgtttcga gcagctctcc atcgacctcg tcatcgtcga
     2881 tgatgaatcc gaagcggtct tccatctcgg tcagcaggcc ggcgacagac atggaatcca
     2941 gctccggcag atggccgaac aggcccgtat cggcatcgaa agcggcaacc cgctccgggg
     3001 ccaggccgag cacgtcggca aggatctggc gcagggtgcg gtcaagctcg gtggtcatct
     3061 gggtagtgtc caacaaacgg aattggcgcg cgtctagcga tcatgacgcg ctgcggcaag
     3121 gcgccgcaat gcctgccgtg cgaggtgtgg ccaggatcgc gggtcacctg gctgaaaagc
     3181 tgacaggtga tagcggggtc gggccagctc catccagtcg cgtttgtagg catcgtcgcc
     3241 ggtcccgaag tcgaccagct cgacgcgatc gacgtcgatc acatgggcca tcagcgcggc
     3301 ggtaagcgtc gtgccggggg aatgggcggc cgcatcctcg cgataggcaa gcttgtgaat
     3361 gtatgcggtt ccctgttcga cggtccacat ctgcgcggct accggtgtgc cgtcgatccg
     3421 cccgatcccc agccgcagcc gccccgcggc cccttcagcc tcggcaaagg cacgcaggaa
     3481 tgcgggcgag ccttcctcgc ctttccagct ggcggcatag attgcttcgt acgttgccca
     3541 ggcatcggtg gaaaaaccgg tcagtacctc gcacaaggtc cgccccgctt tgcgtttgag
     3601 ggtagagcgc aagtgcccgg ggcgcccccc gagataggcc gcatagtccc gaccgccgat
     3661 cggcagcacg tggttgacat catgcggcga tatcgttgcc tgccatccgg ctgcgcggag
     3721 cgcgtcaagc aaggcttcgg cagtcccgtc ctcacccggt atcccgcgca gctcgatccg
     3781 ccaatggttg cgagcaaggt cgcggaatgc ggccgcaagc agccgggcgg catcggctcc
     3841 cgggctgacc agcggacgcc aacggaaagt gtaccagttc gcgaacggcc ggatgacgcc
     3901 ccttccggcc atcagcggca gaagggccgt gccgtcggac tcgtcacgcg ccagaacata
     3961 tcgcggcgtg atatcgcagt gccgggcgag caggttccac cactcggccc ggtcaaacgg
     4021 atcgcgcgcc gagcgcacca gcccggcgag ggacgggtcc tgcgatgctt tgcgcagatc
     4081 cgaaagatag actacggcga tcacgtaaac cccggcgaaa ttggagcgcg agtaagcccg
     4141 gtgccatccc atgccagccc cgcgatccgg ccgcttgacc atctcgcgct ggcgggccgg
     4201 gatgaagacc ccgcgctcct cctgcgttcg ggtgtcctga cataccagga cttaagagca
     4261 cgtatcgccg cccttagcgg ttggttaagc catcgcctgc ccgaagccgg cacgcgggtc
     4321 gccacctggg cggccaaggg cgagctgact tgcctgatgc cgctggcagc ggcgcgcgcc
     4381 gggcttgttc acgtcccgat caatccgctg ctcaagcgcg cgcaggttac gcatatcctg
     4441 cgcgacagcg gctcggccct gctgatcggc tcgcccgccc ggctggccac cctcgacgcc
     4501 gatgatatcg cgccgggatg tcggaccatg ggcgaggaca tggcactcgc tgaggccgcc
     4561 gcgcttggtc gattcctgcc gccgtcatcc gccgatccgg acgcactggc cgccatcctc
     4621 tacacgagcg gatcgaccgg acgacccaag ggggtcatgc tcagccatgc caatatgtgg
     4681 ctgggggcgg aaagcgtggc ctcctatctc cagctttccc gcgaggatcg tacgcttgct
     4741 gtcctgccgc tgtcgttcga ctatggccag aaccagctgc tttccacctg gtatgccggt
     4801 ggcgccgtgg cgccgctcga ttatctggcg ccgcgcgacg tcgtgaaggc gatagcgcgg
     4861 tatgatatca ccacgcttgc ggccgttccg ccgctgtggg tgcaactcgc cgaacaggat
     4921 tggccgccag aggttgcggg caagctaagg aggctgacca atagcggcgg cgcgctaacc
     4981 gtgggcctgg ttcgccgcct gcgagccctg tttccgcagg cccggatttt cgcgatgtac
     5041 ggtcttaccg aggcgttccg ttcgacatat ctcgatcccg ctttgatcga cagccatccc
     5101 acctcgatgg gccgggccat tccccacgcc gaaatcctcg tgatggccga tgacgagcga
     5161 ctggcggcgg acggagagga gggggagctg gtccactgcg gtccgctggt cgcccacggc
     5221 tactggcacg atgccgagcg aacggcggag cggtttcgcc cggcgccgtc gatctcgacc
     5281 tacggcggca ctgcggtctg gtcgggtgac cgggtacggc gtgacgatga ggggctgctc
     5341 tacttcgtcg gccggcgcga tgcgatgatc aagtcggcgg gcaaccggat cagtccgcag
     5401 gagatcgagg aggcggccct cgccaccggt cttgtggccg aggccgtggc gatcggcgtg
     5461 cccgatccgc gcctgggcca ggcagtccat ctcgtcgtta gggctgctga agaaacctca
     5521 gatgcggaga cagaattgcc tcgcaagctc atggccgaat taccgaattt catgcagcca
     5581 aaggtcattc actggcgtcc ggccctgccg gtcggaccca acggcaagat tgaccgggcg
     5641 ggtctcgcgg cggagttgtg cgaatgaagc ctcttggccc gataccggag ggattcagat
     5701 ccaaagaggg cgttcttcaa gtagattctc aagatgttcg gggtcttgtc gccgaggccg
     5761 gcggtacacc gttgttcgtc tattcgagag caatgattgc ggatcgcgtg gccaggctcc
     5821 gcgccgcctt gcccgaacgt gtcgccttac attatgccat aaaagcaaac ccattttcac
     5881 cattacttca tgtaatgtct gaactggtag acgggttcga cgtagcctcg ggcggcgaac
     5941 ttcagaaatt ggttgaactt gatatcgccg cctctcggat cagcttcgcc ggccctggca
     6001 agcgcgatgc cgagctgacg gcagccatcg ctgccggggt caccatcaac ctagagagcg
     6061 agggcgaggc ggagcgcgcc ttgcgccttg cggacgaggc cgggatcaga ccccggctcg
     6121 ccatcagggt aaacccggat tttgacctca agggttcggg catgaagatg ggcggcgggg
     6181 c
//
LOCUS       DATNBV010000217         2185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_139219,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000217 DATNBV010000000
VERSION     DATNBV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139219"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1661
                     /locus_tag="VN627_05305"
     CDS             168..1661
                     /locus_tag="VN627_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HXD02708.1"
                     /translation="MTIGARIAAIIESEPDRRLLEWHGQWFTRGHLARFAGDMNAVLD
                     ARQIEPGTAVALAGRNRPSHCFATLTLTARGLPVSMLYAFQSAESLARDLTSTRFAVA
                     IIDEQDFAPEVREAANASGTTVIVLRPLPTDGFAVVDPDVPVGKGAYRLPGPGIEILS
                     SGTTGLPKRLFHPAERLFRALYGETETPDAPPGLVMWPTSGIGGNMQLISAIIKGIGI
                     VLLEKFEAWPLAEAIKRNKINSLGFTPAMLRMFLDADIPPEYLESVPSIVGGAGPIDP
                     DKIDEFERRYGKPILWAMGATEFCGTIIAWDMDSYRQMWAHKRGSSGRAVAGVELRIT
                     DPDTGEELPRNSLGRLSAKVEAVGDEWIVTNDLARIDEDGYVFFEGRADGAIMRGGFK
                     IVPEKVCQTLRQHPDVAEAALIGIPDERLGEVPVAVIEPRPGHTPSAEDLAAFARAHL
                     AAPFIPVKWVFVDRLPYTASTKVAVGEVRKLLFARLGIVDPKAKLPA"
     gene            complement(1740..>2185)
                     /locus_tag="VN627_05310"
     CDS             complement(1740..>2185)
                     /locus_tag="VN627_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXD02709.1"
                     /translation="FAKALGADEVLDYSQDNWREALKALTGGRGVDVVADPVGGKLFE
                     PAFRSLAWRGRHIVIGFAGGDIPRLPANLPLLKGAALIGADIRQFSIYEPELAAANAA
                     EIERWCEEGLRPPLGTVFDLADFHDAMTTAMSGQSLGKVIVRIGG"
BASE COUNT          377 a          756 c          709 g          343 t
ORIGIN      
        1 cacattgtcg acaacgactt cctcaacggc accaccatcc gcatcgacgg tgcccagcgc
       61 ttcggcttga agtaagcaat gggtcctgcc cgcagcggcg gcgagcgtcg ccgcgacggg
      121 caggacgagc taggccctaa gcgaccgacg caggtggaga gacccgcatg accattggcg
      181 cacgcattgc cgccatcatc gaatccgagc ccgaccggcg gttgctggaa tggcacggcc
      241 aatggttcac gcgcgggcac ctggcccgat tcgcgggcga catgaacgcg gtgctcgatg
      301 cccggcagat cgagcccggc accgccgtgg ctctggccgg gcgcaaccgg ccctcacact
      361 gcttcgcgac cctgacactg accgctcggg gcctgccggt cagcatgctc tacgcgttcc
      421 agtccgccga gtcgctggcg cgggacctga cgagcacccg gtttgccgtg gcgatcattg
      481 atgagcagga ttttgctccc gaagtccgcg aggcggccaa tgccagcggc accacggtga
      541 tcgtgctgcg cccgctgccg accgatggct tcgccgtggt cgatcccgac gtgccggtcg
      601 gcaagggcgc ttaccgcctg cccggacccg gcatcgagat cctcagcagc gggaccaccg
      661 gcctgcccaa gcgcctgttc catccggctg aacgcctgtt ccgcgccctc tacggcgaga
      721 cggaaacgcc tgatgcgccg cccgggctgg tcatgtggcc gaccagcggc atcggcggca
      781 acatgcagct gatcagcgcc atcatcaaag gcatcggcat cgtgctgctg gaaaagttcg
      841 aagcctggcc gcttgccgag gcgatcaagc gcaacaagat caacagcctc ggttttactc
      901 ccgccatgct gcgcatgttc ctggatgccg atatcccgcc cgaatacctc gaatcggtgc
      961 catccatcgt cggcggcgcc gggccgatcg atcccgacaa gatcgacgag ttcgagcggc
     1021 gctacggcaa gcccattctc tgggccatgg gagcgaccga gttctgcggc acgatcatcg
     1081 cgtgggacat ggacagctac cggcagatgt gggcccacaa gcgcggcagc tcgggccgcg
     1141 cggtggcggg cgtggaactg cgcatcaccg atcccgatac cggcgaggaa ttgccccgca
     1201 attcgctggg tcgcctgtct gccaaggtcg aagccgtggg cgacgagtgg atcgtcacca
     1261 atgacctcgc ccgcatcgat gaggacggct acgtgttctt cgaaggccgc gcggacgggg
     1321 caatcatgcg cggcggattc aagattgtgc cggaaaaggt ctgccagacc ctgcgccagc
     1381 acccggacgt ggccgaggcc gccttgatcg gcattcccga cgagcggctc ggcgaagtgc
     1441 ccgtagccgt catcgaaccg cgcccgggcc atacaccgtc ggccgaggac ctggctgctt
     1501 ttgcccgggc ccaccttgcc gccccgttca tcccggtgaa gtgggtcttc gtcgatcggc
     1561 taccctacac ggcctcgacc aaggtcgccg taggcgaggt gcgcaagctg ctgttcgccc
     1621 ggctgggtat cgtcgatccc aaggccaagc tccccgcctg atgcctcagg cgcgagggtc
     1681 gcgcgggccg tcgccatccg cgcgaacagc ccaaggcctg tgcaaaggcg ccaaccggcc
     1741 tacccgccga tgcgaacgat gaccttgcca agcgactggc ccgacatggc cgtggtcatg
     1801 gcatcgtgga aatcagcgag gtcgaatacc gtaccgagcg gcgggcgcag gccctcttcg
     1861 caccagcgtt cgatctcggc ggcgttggcg gccgccagtt ccggctcgta aatcgagaac
     1921 tggcggatat cggcgccgat cagcgcagca cctttcagca gcggcaggtt ggcggggagc
     1981 cggggaatgt cgccgccggc aaagccgatg acgatgtggc ggcccctcca ggccaggctg
     2041 cggaaggccg gttcgaagag cttcccgccg accggatccg cgacgacgtc gaccccgcgc
     2101 ccgccggtca gcgctttcaa agcctcgcgc cagttgtcct ggctgtaatc gagtacctcg
     2161 tcggccccca gggccttggc aaaat
//
LOCUS       DATNBV010000218         3019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2050669,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000218 DATNBV010000000
VERSION     DATNBV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050669"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="VN627_05315"
     CDS             <1..1426
                     /locus_tag="VN627_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXD02710.1"
                     /translation="TVNWNYLTEPDDGTAGRRIAQARGKMLGGSSAINGMVYVRGQPQ
                     DYDHWAQLGNPGWSWADVLPLYRRMESFAGGSEATRGRTGPLRISESRPGAVPLFEAV
                     IAAAASLGIARGDFNGAGQDAVGMSQATIARGLRQSTAIAYLAPARKRPNLKVVTGAM
                     AERLLLDGRLCTGVRYRIDARVIEAHAREVIVCGGSINSPQLLEVSGIGQGERLRALG
                     LPVHHDLSGVGENLRDHYLSRMHYTTSAPRLTMAHRARGLGLAREILRYGLTRTGLLA
                     ETVVPLRLYARLSPGAASPETGISVTPFLFEVQGNNWGVAKRDGFTITVHPLRPESTG
                     SVHAVSPDPGRHPAIRMNYLSAWTDRELTVAGIRKVRELVATPAMAAIVGEELQPGPG
                     AQDDEALLDWVRRTSETGYHPVGTCKMGSDPLAVVDHRLRVRGIAGLRIADGSIMPTI
                     VSGNTNAACIMIGEKCADLVRADG"
     gene            1506..1976
                     /locus_tag="VN627_05320"
     CDS             1506..1976
                     /locus_tag="VN627_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02711.1"
                     /translation="MACPADLPARIALMEAGLELVADRVGDITGPVVARLYRRCPAAR
                     ASFVRLGLGRPDALAAQMVETTLHCLMSWPGDRAMVEITLRETLPHHCLTLGIAPAVF
                     RALAEETAHLIIAAIPADCAAERAVWEDIRTGILGAIAEAERSPLIARAAVQAA"
     gene            2090..2872
                     /gene="purC"
                     /locus_tag="VN627_05325"
     CDS             2090..2872
                     /gene="purC"
                     /locus_tag="VN627_05325"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXD02712.1"
                     /translation="MSRRRQIYEGKAKILYEGPEPGTLIQYFKDDATAFNAQKKGTIN
                     GKGVINNRISEYVFTRLSHIGIPTHFIRRLNMREQLVRQVEIIPIEVVVRNVAAGSIS
                     KRLGIPEGEPLPHTLIEYYYKDDALGDPMIAEEHIACFGWASNEEMQDISSMAIRVND
                     FMVGMFAAINIRLVDFKLEFGRIWDGDYSRVILADEISPDGCRLWDMITGEKLDKDRF
                     RRDLGGEEEAYQEVARRLGLLENDGGPSEVFDLSKHRKLRGK"
BASE COUNT          505 a         1080 c          943 g          491 t
ORIGIN      
        1 cacggtcaac tggaactacc tgaccgagcc ggacgatggc actgccgggc gccgcattgc
       61 tcaggcgcga ggaaagatgc tcggcgggtc ctcggcgatc aacggcatgg tctacgtgcg
      121 cggccagccg caggactatg accactgggc ccagctcggc aatcccggct ggtcctgggc
      181 cgatgtcctg ccgctttacc gccgcatgga aagcttcgcc gggggcagtg aggcgacgcg
      241 cggacgcacc ggccccctgc ggatcagtga gagccggccc ggcgcggttc ccctgttcga
      301 agcggtgatt gcagcggccg ccagcctggg catcgcgcgc ggcgatttca acggcgccgg
      361 gcaggacgcg gtcggcatgt cgcaggccac catcgcccgc ggcctgcgtc agagcaccgc
      421 catcgcctac ctcgctccgg cgcgtaaacg ccccaacctc aaggtggtca cgggcgccat
      481 ggccgaacgg ctcctgctcg atggccgact ctgcaccggg gtgcgctacc gcatcgacgc
      541 ccgcgtgatc gaggcccacg cccgtgaagt catcgtctgc ggcgggtcga tcaattcgcc
      601 gcaattgctg gaggtttccg gcatcggcca gggcgaacgg ctgcgcgcgc tcggcctgcc
      661 cgtccaccac gacctgtccg gtgtcggcga gaacctgcgc gatcactacc tctcgcgaat
      721 gcactacacc accagtgcac cccggctgac catggcccac cgcgcgcgtg ggctgggcct
      781 ggcacgcgaa atcttgcgct acggtctcac ccgcaccggg ctgctggcgg aaacggtcgt
      841 acccctgcgg ctttacgccc gcctgagccc aggcgccgcc tcgcccgaaa ccgggatctc
      901 ggtcaccccc ttcctcttcg aggtccaggg caataactgg ggcgtcgcta agcgcgatgg
      961 cttcaccatc acggttcacc cgctccggcc tgaaagcacc ggatcggtcc atgccgtctc
     1021 gcccgatccg ggacgccacc cggcgatccg catgaattac ctctcggctt ggaccgaccg
     1081 cgaactcacc gtcgccggga tccgcaaggt gcgcgaactt gtcgccacgc cggcgatggc
     1141 ggcgatcgtc ggcgaggaac ttcagcccgg ccccggcgcg caggacgacg aggccctgct
     1201 cgactgggtc cgccgaacct cggaaaccgg ctaccacccg gtcggcacct gcaagatggg
     1261 cagcgatccc ctggccgttg tcgatcaccg cctcagggtc cgcgggatcg ccggactgcg
     1321 cattgccgat ggttcgatca tgccgaccat cgtgtcgggc aacaccaacg ccgcctgcat
     1381 catgatcggc gaaaagtgcg ccgacctcgt ccgcgccgat ggctgagcgg gcttgatttc
     1441 cgtcaattta tccaaacgcc aggcgtgtaa cattcacggt tgttgaatgt ttggagcacc
     1501 gcccgatggc atgcccggcc gacctgcccg cgcgtatcgc cctgatggaa gcggggctcg
     1561 aactcgtggc cgatcgggtg ggcgacatta ccgggcccgt ggttgcccgg ctctatcggc
     1621 gctgccccgc tgcgcgcgcc agtttcgtcc gtcttggtct cggccggccc gatgcattgg
     1681 ccgcgcagat ggtcgaaacc acgctacatt gcctgatgag ctggccgggc gaccgggcca
     1741 tggtggagat caccctgcgc gaaacgctcc cccaccattg tctgaccctg ggtatcgccc
     1801 ccgccgtgtt ccgagcgctc gccgaagaaa cggcccacct gatcatcgcc gccatcccgg
     1861 ccgactgcgc cgccgagcgc gccgtgtggg aggacatccg cacgggcatc ctcggcgcga
     1921 tcgccgaggc cgaacgctcg cccctcattg cccgtgccgc ggtccaggcc gcgtgatcct
     1981 gcgcgtcgcc ggcgccatcc cgtcccgcga cattgcattt cccgccccat ggggatatgg
     2041 gccgcccttc ttttcagaca tggggcctac ggccccggag gaagtgcccg tgtcccgtcg
     2101 ccgccaaatc tacgaaggca aggccaagat cctctacgaa ggtcccgagc cgggcacgct
     2161 gatccagtat ttcaaggacg atgccaccgc gttcaatgcc cagaagaagg gcacgatcaa
     2221 cggcaagggc gtgatcaaca accgcatcag cgaatatgtg ttcacgcggc tgtcgcacat
     2281 cggcatcccc acccacttca tccgccgtct caacatgcgc gagcagttgg tccgccaggt
     2341 ggaaatcatc ccgatcgagg tcgtcgtgcg caatgttgcc gccggctcga tctccaagcg
     2401 cctcggcatt cccgagggtg agccgctgcc ccacaccctg atcgagtatt actacaagga
     2461 cgatgcgctg ggcgatccga tgatcgccga agaacacatc gcttgcttcg gctgggcctc
     2521 gaacgaagag atgcaggaca tctcctccat ggcgatccgg gtcaacgact tcatggtcgg
     2581 catgttcgcg gcgatcaaca tccgtctggt cgatttcaag ctcgagttcg gccgcatctg
     2641 ggatggcgac tacagccgcg tgatcctggc cgacgagatc agccccgacg gctgccgcct
     2701 gtgggacatg atcaccggcg agaagctcga caaggaccgc ttccgccgcg atctcggcgg
     2761 tgaagaggaa gcctaccagg aagtcgcccg ccgcctcggc ctgctcgaga acgacggtgg
     2821 cccgagcgaa gtgttcgatc tctccaagca ccgcaagctg cgcggcaagt aagctgcgga
     2881 ccctccccgc tggcggggaa gggaccgcct acccccccgg cggtcgggcc aaccgttcgt
     2941 ggtgggctac ctgtcgggct gcgagacgcc gaggcggcct ctccgcagcg tcgcgcaacc
     3001 ttcacaccca tgaccgctg
//
LOCUS       DATNBV010000219         3051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1712008,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000219 DATNBV010000000
VERSION     DATNBV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712008"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VN627_05330"
     CDS             <1..554
                     /locus_tag="VN627_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HXD02713.1"
                     /translation="AALAVIAPVAFFTVLHDYQRQRVFVFLDPAKDPLGTGYHILQST
                     IAIGSGGFFGKGFGNGSQSHLDYLPEAHTDFVFATMSEEWGMLGGLFVIGVFAWVLNW
                     ALRTARGASGRFDQLLAMGMAFTMFFYVCINLMMVMGLAPVVGIPLPFMSHGGSSMMT
                     NMICVGTIMAVANWSREGPRSLS"
     gene            674..749
                     /locus_tag="VN627_05335"
     tRNA            674..749
                     /locus_tag="VN627_05335"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:707..709,aa:Lys,seq:ctt)
     gene            899..1975
                     /locus_tag="VN627_05340"
     CDS             899..1975
                     /locus_tag="VN627_05340"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HXD02714.1"
                     /translation="MALLGIFGAPRRYVQGPGAIRELASVRPVLGQRPVAIVDADILP
                     ALEADLSAALGQGEYRILAFRGEITRAQIARLIAELDGFGADIVIGVGGGKGLDAAKG
                     VAKALETPFVAVPTIASNDSPTGRSMAIYDDNHVMIAVEFLPDAPALVVVDTAIIARA
                     PARFLRSGMGDAIAKKFEAERAHAYGSKNGLGGKQTLTALAIGDLCYRTLRQYGVAAM
                     AAAEAHEVNDAFEAAVEANVLMSGLGWESGGLSYAHAVVRGLVQARDAKNNPHGEHVA
                     YGTLVQLAIEGRDDAFIRDVAGFLRDVGLPTSLGELGMADPTDAEIAEIARLTTIGPV
                     GGMIEVRAAPEQIAAAIRRIEAMA"
     gene            complement(1978..>3051)
                     /locus_tag="VN627_05345"
     CDS             complement(1978..>3051)
                     /locus_tag="VN627_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXD02715.1"
                     /translation="VVVVGTRFGAEAHIPGFKAAGYDVVGLVGRDRAKTEAIAAHYGT
                     RGFAGLDAALAEKPDVVSIASPPHLHYDLAKQALEAGVHVVCEKPFTRTLREAAELRD
                     LAAERGLVGVLCHEFRYVPSTALIGRLLAEGAIGQPRMATLIAHSGYVADVTMPRPDW
                     WFNKELGGGWLGASGSHGIDRIVDWFGDMAGVSAQRVCASARQDGSADDSFDLHFRSR
                     SGVIGTFQSTAAAWGPAVDITRVIGPAGTLWLEDTVQRGIIEVTAGPVHISDAQGTRT
                     IAVPPDLEVPPLAVDSRWGHSSALFAQQFREVARLIAGEAPTMRYPRSTTFADGYRNM
                     LVLEAAYRSAARGGEWIDVTEPA"
BASE COUNT          467 a         1036 c         1032 g          516 t
ORIGIN      
        1 gcgcggccct tgccgtgatc gcgcccgtgg ctttcttcac cgtgctgcac gactaccagc
       61 gccagcgggt gttcgtgttc ctcgatcccg ccaaggaccc gctcggcacg ggctaccata
      121 tcctgcaatc gacaatcgcg atcggctctg gcgggttctt cggcaagggc ttcggcaacg
      181 gttcgcagag ccacctcgat tatctcccgg aagcgcacac cgacttcgtc ttcgccacga
      241 tgagcgagga atgggggatg ctgggcggcc tcttcgtgat cggcgtgttc gcctgggtgc
      301 tcaactgggc cctgcgcacg gcgcgcggcg ccagtggccg gttcgaccag ctgctggcca
      361 tgggcatggc cttcaccatg ttcttctatg tgtgcatcaa cctgatgatg gtgatgggcc
      421 tcgcccccgt ggtgggcatt ccgctgccct tcatgagcca cggcggctcg tcgatgatga
      481 cgaacatgat ctgcgtgggc accatcatgg ccgtggccaa ctggtcacgc gaggggccgc
      541 gcagcctctc ctgacgtgcc ccggttattt ttgccggggc ggcgaaaaag ccccttccca
      601 tccggaattt cgccgctata gggagcgctc ccgagcggca ctgcccctca ggaaactccg
      661 gtcaggccgg tgtggacgca tagctcagtt ggtagagcag ctgactctta atcagcgggt
      721 cctaggttcg agccctagtg cgtccaccac cccccgcacc cccttcttcg ccctcctcca
      781 tcacgctgcc gatgggcctt gcccggcgat tgcgctgtgg ccacaagcga gcctatcggc
      841 gggcgatagt ggcagcggct caggttcgtc cggtcgcgtc agcaggagtt tggcgggtat
      901 ggcattgctt ggcattttcg gggcgccccg gcgctatgtg cagggccccg gcgcgatccg
      961 ggaactggcc tcggtgcgcc cggtgctggg ccagcggccg gttgccatcg tcgatgccga
     1021 tatccttccg gcgctggagg ctgacctgtc tgccgcgctg ggccaggggg aataccgcat
     1081 cctcgccttt cgcggcgaga taacccgggc ccagatcgcg cggctcattg ccgaacttga
     1141 cggtttcgga gccgacatcg tgatcggcgt cggcgggggc aagggcctcg atgcggccaa
     1201 gggggtggcg aaggcgctgg agacgccctt tgtcgccgtg cccaccattg cttccaacga
     1261 ttcgccgacc gggcgatcga tggccatcta cgacgacaac cacgtcatga tcgccgtcga
     1321 attcctcccc gacgccccgg cgctggtcgt cgtcgacacg gcgatcattg cccgcgcccc
     1381 ggcgcggttc ctgcgctcgg gcatgggcga tgccatcgcc aagaaattcg aggccgaacg
     1441 cgcccatgcc tatggttcga agaacggtct tggcggcaag cagacgctta ccgcgctcgc
     1501 catcggcgac ctgtgctacc gcaccctgcg ccagtacggc gttgccgcca tggccgcagc
     1561 cgaggcccat gaggtcaacg atgcgttcga ggcggcggtg gaggccaatg tcctgatgtc
     1621 gggcctgggc tgggaaagcg gcgggctgtc ctatgcccat gcggtggtgc gcgggctggt
     1681 ccaggcgcgc gatgccaaga acaatcccca tggcgaacat gtcgcctacg gcacgctcgt
     1741 ccagctggcc atcgaagggc gggacgatgc cttcatccgc gatgtcgccg ggttcctgcg
     1801 cgacgttggc ctgcccacat cgctgggcga gctgggcatg gccgatccga ccgatgcgga
     1861 aatcgccgag atcgcccgtc tcaccaccat cggtccggtc ggcgggatga tcgaagtgcg
     1921 cgccgcgccc gaacagatcg ccgcggcgat ccgccggatc gaggccatgg cctgacgcta
     1981 ggccggctcg gtaacgtcga tccattcgcc gccgcgcgcg gcggaccgat aggcggcttc
     2041 aagcaccagc atgttgcggt agccgtcggc gaaggtcgtc gaccgggggt aacgcatggt
     2101 cggcgcctcg ccggcgatca ggcgggccac ttcgcgaaac tgctgggcaa acagggccga
     2161 tgaatgcccc caccggctat cgaccgccag tggcggcacc tcgagatcgg gcggcacggc
     2221 gatggtgcgg gtgccctggg cgtcagagat gtggaccggg cctgccgtca cctcgatgat
     2281 gccgcgctgg accgtgtctt ccagccacag cgtgccggcc gggccgatca cgcgggtgat
     2341 gtccactgcc gggccccagg cggcggccgt actctggaag gtgccgatca cccctgagcg
     2401 cgaccggaag tggagatcga aactgtcgtc ggccgagccg tcctgccggg cgctggcgca
     2461 gacgcgctgg gcgctgaccc cggccatgtc gccgaaccag tcaacgatcc ggtcgatccc
     2521 gtgcgagccg gaagcgccga gccagccccc gcccagctcc ttgttgaacc accagtcggg
     2581 ccggggcatg gtgacatcgg cgacatagcc gctgtgggca atcagggtcg ccatgcgcgg
     2641 ctggccgatc gcgccttcgg ccagcaggcg gccgatcagt gccgtggacg gaacatagcg
     2701 aaattcatgg cacagcacgc cgaccagccc gcgctcggcc gcgagatcgc gcagctcggc
     2761 ggcttccctc agggtacgcg tgaacggctt ttcgcagacc acgtgaaccc cggcctcgag
     2821 cgcctgcttc gcgaggtcgt agtgcaggtg cggcgggctt gcgatggaga ccacgtcggg
     2881 cttctccgcg agggcggcat ccagcccggc aaagccccgg gtgccataat gcgcggcaat
     2941 cgcctcggtc ttggcgcgat cgcggccgac gaggccgacg acatcgtagc ccgctgcctt
     3001 gaagccggga atgtgcgcct cggcgccgaa ccgcgtgccg acgaccacga c
//
LOCUS       DATNBV010000220         4639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_140876,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000220 DATNBV010000000
VERSION     DATNBV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140876"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VN627_05350"
     CDS             <1..260
                     /locus_tag="VN627_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXD02716.1"
                     /translation="GWPATEATARHAIIVTQSGGQVRGLIVHSVSDIVAITDDALQPP
                     PAPSGDSVVGFLEGLVTHDERMIMVLDLKALNADEAALEAA"
     gene            257..586
                     /locus_tag="VN627_05355"
     CDS             257..586
                     /locus_tag="VN627_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02717.1"
                     /translation="MNKPGPVYSLAGAVFVPATGSGHADAHLKEMAAMLGEISTQLEK
                     LGEVLCHDPEFVGRHVQELQAIDLIAQKQRALAAVLGAECPTSALAGVGLEEVVDRFK
                     ALASRFG"
     gene            801..1262
                     /locus_tag="VN627_05360"
     CDS             801..1262
                     /locus_tag="VN627_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HXD02718.1"
                     /translation="MSQTASLNSRIIESLYVEGLMLSDEVRAAFDPAHRTAGDPSSTA
                     AIALSCEALRTTTRMMHAVAWLLNHRAHLRGEISEFQLRRHGRIAANLPGSDSRRMAQ
                     LAPQMQDLIRATERFYARLVRLDSGWRERTGPTRSVPAVQRLRERLAQAAP"
     gene            1300..2175
                     /locus_tag="VN627_05365"
     CDS             1300..2175
                     /locus_tag="VN627_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXD02719.1"
                     /translation="MEFPPQPWPVGQAELLEPLVHRVLAPNPGPFTFTGTETYLIGSA
                     GALAVVDPGPNDPDHVAALIRLIGDRPVAAICCTHTHKDHSPAAAPLAAATGAPVIGC
                     APLVLDSIGPRGDAPFDPSYAPDRVLAGGESIAGPDWTLTAVATPGHTSNHLCFALKE
                     SGALFTGDHVMGWSTSVVIPPDGDMAAYMASLEKLHARADRVYYPAHGPQVDKPRQLV
                     RGMIGHRRQRERQILKLLAQSEARIVELVPQMYKGIDERLHGAAGMSVLAHLVELEQR
                     GTVSRREDIWMLLEV"
     gene            complement(2305..>4639)
                     /gene="acnA"
                     /locus_tag="VN627_05370"
     CDS             complement(2305..>4639)
                     /gene="acnA"
                     /locus_tag="VN627_05370"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HXD02720.1"
                     /translation="LGGDTSKINPLVPVNLVIDHSVMVDEFGTPKAFEQNVEIEYQRN
                     MERYDFLKWGSKSLNNFYAVPPGTGICHQVNLENIAQSVWTSADQNGQTVAYFDTCVG
                     TDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFRFTGELQEGVTATDLV
                     LTCTNMLRKHGVVGRFVEYFGPGLAGLTLADRATLANMAPEYGATCGFFGIDDKTLDY
                     LRLTGREESQIALVEAYAKEQGFWIDPAVEPVFTSTLELDLSTVVPSLAGPKRPQDRV
                     SLPEVDDVFNADMAQTYKKTYERVPVEGKDFTIGDGDVTIAAITSCTNTSNPGVLVAA
                     GLVAKKADELGLKPKPWVKTSLAPGSQVVTDYLERAGLQKHLDNIGFNLVGYGCTTCI
                     GNSGPLAEPISKAINEHGLVAAAVISGNRNFEGRVSPDVRANFLASPPLVVAYALKGT
                     VVEDFISTPIGTSKDGKEVFLKDIWPTNDEVYTTMAGCMDRAMFQARYADVYKGDAHW
                     QAINVTGSETYQWRAGSTYIANPPYFEGMEMTPAPVSDIIEAKPLLILGDSITTDHIS
                     PAGSIKADSPAGKWLMEHQVAKADFNSYGARRGHHEVMMRGTFANIRIKNEMVPGIEG
                     GMSRYGEEVLPVYDVAEKNKADGTPMVVIAGKEYGTGSSRDWAAKGTNLLGVRAVIVE
                     SFERIHRSNLVGMGVLPLQFKEGESRTSLGLCGDDTFTIKGVAALQPRQDVEVEVTRK
                     DGSKFSFTALCRIDTANEVEYFMNGGILQYVLRKLAA"
BASE COUNT          749 a         1510 c         1582 g          798 t
ORIGIN      
        1 tgggctggcc ggccaccgag gccaccgcgc gccatgccat catcgtcacc cagtcgggcg
       61 gacaggtgcg cgggctgatc gtccactcgg tcagcgatat cgtcgccatc accgatgatg
      121 cgctacagcc cccgccagca ccctcgggcg atagcgtggt gggtttcctc gagggcctgg
      181 ttacgcacga cgagcgcatg atcatggtgc tggacctgaa ggctctcaat gccgacgagg
      241 ccgctctgga ggccgcatga acaagccggg ccccgtctat tcgctcgctg gcgccgtgtt
      301 cgtgccggca accggctctg gccacgccga tgcgcacctc aaggaaatgg cagcgatgct
      361 gggcgagatc agcacacagc ttgaaaagct tggcgaggtg ctgtgccacg atccggaatt
      421 cgtcggccgg cacgtgcagg agctgcaggc gatcgacctg atcgcccaga agcagcgggc
      481 gctggccgcc gtgctcggcg ccgaatgccc gaccagcgca ctggccggcg tcggcctcga
      541 ggaagtggtc gaccgattca aggcgcttgc cagccggttc ggctaggact ggcgagacgc
      601 aaggcgaagg gcccgagccg ctcaagcggc gcgggccctt cgccttgcgt gacaagccgc
      661 acccgacaat tgatatgtcg accgggggat ccgaaacccg ttctgcggcg ttccaatccg
      721 gcacagtccc gaaatgaccg gtgaataggc tgtaatggcg attgaatccg ggaatcttgc
      781 ggcggtatcg gcagggagtc atgtcgcaga cggccagcct caactcgcgg atcattgaat
      841 ccctttatgt cgaaggactc atgctgtccg acgaggtccg tgccgccttc gatcctgcgc
      901 accggacagc cggggatccg agctccacgg cggccatcgc cctttcgtgc gaggcgctgc
      961 gcacgaccac gcggatgatg catgcggttg cctggctgct caatcaccgc gctcacctgc
     1021 gcggcgagat cagcgagttc cagctgcgcc gccatggccg gattgccgcc aacctgccgg
     1081 gctccgactc gcggcggatg gcgcaactgg ccccacagat gcaggacctg atccgcgcga
     1141 ccgaacggtt ctacgcgcgg ctggtgcggc tcgacagcgg ctggcgcgag cggacggggc
     1201 ccacgcgcag cgttcccgcg gtccagcgcc tgcgcgagcg gctggcccaa gccgcgccct
     1261 gacctcgaca gggcgccgcg ggatcgccat atccggtcca tggaattccc gccccagcca
     1321 tggccggtcg gccaagccga actgctcgaa ccgctcgtgc accgggtgct tgcccccaat
     1381 ccgggcccgt tcacctttac tggcacggaa acctacctga tcggctcggc cggggccctg
     1441 gcggtggtcg atcccggtcc gaatgatccg gaccacgtcg ctgccctgat ccgcctgatc
     1501 ggcgaccggc ccgtggcggc aatctgctgc acccataccc acaaggacca ctcgcccgcc
     1561 gccgcgccgc tcgccgcagc gacgggtgcc ccggtgatcg gctgcgcgcc gctggtgctc
     1621 gacagcatcg gaccgcgcgg cgatgctccg ttcgatccca gctatgcgcc cgaccgggtg
     1681 ctggccggtg gcgagagcat cgccggtccg gactggacgc tcacggcagt ggcgaccccg
     1741 ggccatacgt ccaaccacct gtgcttcgcc ctcaaggaaa gcggggcgct gttcaccggc
     1801 gaccatgtga tgggctggtc gaccagcgtg gtgatcccgc ccgacggcga catggctgcc
     1861 tatatggcca gcctcgagaa gctccacgcc cgcgccgacc gggtctatta cccggcccac
     1921 gggccccagg tcgacaagcc gcggcaactg gtgcgcggca tgatcggtca ccgccgtcag
     1981 cgcgaacgcc agatcctgaa gctgctggcg caaagcgagg cgcggatcgt cgaactggta
     2041 ccgcagatgt acaagggcat cgacgagcgg ctgcatggcg cggccggcat gtcggtgctc
     2101 gcccacctgg tcgaactcga gcagcgcggc acggtcagcc ggcgggaaga catatggatg
     2161 ctgttggaag tttgacgccg ccattctccg gcgctggcag gccgatgcct gccaggcggt
     2221 cccggaacta atctttgagc gccaaacaaa tgaaaggccc gccgttgccg gcgggccttt
     2281 gctgttccgg caatgccgtg gcgatcaggc tgccagcttg cgcagcacgt actgcaggat
     2341 gccgccgttc atgaagtact cgacctcgtt ggcggtatcg atgcggcaca gggcggtgaa
     2401 gctgaacttg gagccatcct tgcgggtgac ctccacttcc acgtcctggc gcggctgcag
     2461 cgccgcgacg cccttgatgg tgaaggtgtc atcaccgcac aggcccagcg aggtgcggct
     2521 ctcgccctcc ttgaactgca gcggcagcac gcccatgccg accaggttcg agcggtggat
     2581 gcgctcgaag ctttcgacga tcacggcgcg aacgcccagc aggttggtgc ccttggccgc
     2641 ccagtcacgg ctggagccgg tgccgtattc cttgccggcg atgaccacca tcggcgtgcc
     2701 gtcggccttg ttcttctcgg ccacgtcgta gaccgggaga acctcctcgc cgtagcggct
     2761 catgccgccc tcgatgccgg ggaccatctc gttcttgatg cggatattgg cgaaggtgcc
     2821 gcgcatcatg acttcgtggt ggccacggcg cgcgccgtag gagttgaagt cggccttggc
     2881 gacctggtgc tccatcagcc acttgcccgc cgggctgtcg gccttgatcg agccagccgg
     2941 gctgatgtgg tcggtggtga tcgaatcgcc gaggatcagc aggggcttgg cctcgatgat
     3001 gtcgctgacc ggggccgggg tcatctccat gccctcgaag tagggcggat tggcgatata
     3061 ggtgctgccg gcgcgccact ggtaggtttc cgagcccgtg acattgatcg cctgccaatg
     3121 cgcgtcgccc ttgtagacgt cggcatagcg ggcctggaac atggcgcggt ccatgcagcc
     3181 ggccatggtg gtgtagacct cgtcgttggt cggccagatg tccttgagga agacttcctt
     3241 gccatccttg ctcgtgccga tcggggtaga aatgaagtcc tccaccaccg tgcccttgag
     3301 cgcataggca acgaccagcg gcgggctggc gaggaagttg gcgcgcacgt cgggagagac
     3361 gcggccctcg aagttgcggt tgcccgagat caccgcggcg gccaccaggc cgtgctcgtt
     3421 gatcgccttg gaaatcggct cggccagcgg gcccgagttg ccgatgcagg tggtgcagcc
     3481 atagccgacg aggttgaagc cgatgttgtc gaggtgcttc tgcaggccgg cgcgctcaag
     3541 gtagtcggtg acgacctgcg agccgggggc gagcgaggtc ttgacccacg gcttgggctt
     3601 gaggccaagc tcgtcggctt tcttggcgac gaggccggcg gccaccagca cgcccgggtt
     3661 cgaggtgttg gtgcacgagg tgatcgccgc gatggtcacg tcgccatcgc cgatggtgaa
     3721 gtccttgccc tcgaccggaa cgcgctcgta ggtcttcttg taggtctggg ccatatcggc
     3781 gttgaacacg tcgtcgacct cgggaagcga gacgcggtcc tgcgggcgct tggggccggc
     3841 gagcgacggc acgacggtcg acaggtccag ctcaagcgtg gaggtgaaga ccggctcgac
     3901 ggccggatcg atccagaagc cctgctcctt ggcataggct tcgaccaggg cgatctggct
     3961 ttcctcgcgg ccggtgaggc gcaggtagtc gagcgtcttg tcgtcgatgc cgaaaaagcc
     4021 gcaggttgcg ccgtattcgg gggccatgtt ggccagcgtc gcgcggtcgg ccaaggtcag
     4081 gccggcgagg cccgggccga agtattcgac gaatcggcca accacgccat gcttgcgcag
     4141 catgttggtg caggtcagca cgaggtcggt ggcggtcacg ccttcctgaa gctcgccggt
     4201 gaagcggaag ccgacgactt cggggatcag catcgagacc ggctggccga gcatggccgc
     4261 ctcggcctcg atcccgccga cgccccagcc cagcacgccg aggccattga tcatggtggt
     4321 gtgcgaatcg gtgccgacgc aggtgtcgaa ataggccaca gtctggccgt tctggtccgc
     4381 cgacgtccac accgactggg cgatgttctc gaggttgacc tggtggcaga tgccggtgcc
     4441 cgggggcacg gcgtagaagt tgttgagcga cttggagccc cacttgagga agtcgtagcg
     4501 ctccatgttg cgctggtatt cgatctcgac gttctgctcg aaggccttgg gcgtgccgaa
     4561 ctcgtcgacc atgaccgagt ggtcgatgac gaggttcaca ggcaccagcg ggttgatctt
     4621 gctggtgtcg ccgccgagc
//
LOCUS       DATNBV010000221         5197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1403407,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000221 DATNBV010000000
VERSION     DATNBV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403407"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VN627_05375"
     CDS             <1..467
                     /locus_tag="VN627_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HXD02721.1"
                     /translation="EAQIVAVMVHRPGAQLLLASNIGRGFVAESGELLAETRKGRGVM
                     STKPGVRLALVREIPESHDHVAVVGENRKLVVFSLSEMPVLAKGQGVTLQRYRDGGMA
                     DAVTFKLDEGLSWKMGGESGRTRTEGDMRLWKVARGAAGRMPPNGFPRDNKF"
     gene            complement(471..1004)
                     /locus_tag="VN627_05380"
     CDS             complement(471..1004)
                     /locus_tag="VN627_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02722.1"
                     /translation="MTGAPALLTAPAFAQAAPSAAVQVTAGATVYGPQGAEVGKIEKV
                     DGPNAVINTGSHSAAIPVSAFGKNEKGLLVSMTREQLDAAVEAATAKAAGNLDQALVA
                     GAALHSADGAAMGKISSVSPEGIVTVEREGGSFALKKDMFTTDAQGVALKLTAAQIQA
                     ALAKQQQASASTPAAQP"
     gene            complement(1124..1804)
                     /locus_tag="VN627_05385"
     CDS             complement(1124..1804)
                     /locus_tag="VN627_05385"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HXD02723.1"
                     /translation="MKLPESNVMEPAPLVQYLLSQPLLTLALVSMAVVLAGGLLRHLS
                     PLLGGLVRGLGHIGLFGALMLTIMQVIRVSQGADLSLPQLGMPTQEVSGSETRVPLAR
                     DGHFWIRASVNGVPHRFLVDTGATLTAISPEIAEAARLEPQPLRQPILLRTANGTVPA
                     RMVTINELTLGNVVARDLDAVVAPGMEGTSVLGMNFLSRLAAWRVEGSTMILVPHHPQ
                     GGKPPAES"
     gene            complement(1825..2991)
                     /locus_tag="VN627_05390"
     CDS             complement(1825..2991)
                     /locus_tag="VN627_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HXD02724.1"
                     /translation="MTTLLPDNAWTGREDLAALVTALGPGNGRYVGGAVRDTLLGLAV
                     KDIDMATPLAPGAVIERLAAAGIRTVPTGIAHGTVTAILPEGPVEITTLRHDVSTDGR
                     RATIAYAQDWQDDAARRDFTINALYADPLTREVFDWFGGLDDLAHRRVRFIGDARQRI
                     REDHLRILRYFRFQARFGSEPADPESESACTELAATLKGLSRERIAMELLNLLALPDP
                     APTVSRMAQLGILTVILPEGDPAGLAALVAAERRTGAAADPLRRLAALLPADPAVAEQ
                     VGARLRLSTAQRKRLVTAAGRQGNADGARALAYRLGREEAIDRLLLDGQDIAALSGWE
                     IPRLPIKGADIVARGVGAGPEVARVLRAVEARWVAEGFPGAQRVQELLGEALAP"
     gene            complement(2988..3593)
                     /locus_tag="VN627_05395"
     CDS             complement(2988..3593)
                     /locus_tag="VN627_05395"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HXD02725.1"
                     /translation="MTELHDRLTRLYEAGHARSDIDVIVNPWSQAEGYYRPSAVLAAI
                     TDRPDPGVLLLYRPKTMRSHAGEVVLPGGKVDPGETFIEAAVREAHEEMGIDPAAVRV
                     IGPGDMLRTGTMYQINPVLALVPGDITITPNPAEVSDWFEAPLRHVLDPANHVEDPYV
                     FGEKTVHVWTIHWNGHCIWGSTAMVLINLARRLNWTGARAG"
     gene            complement(3586..4179)
                     /locus_tag="VN627_05400"
     CDS             complement(3586..4179)
                     /locus_tag="VN627_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HXD02726.1"
                     /translation="MSALFTRIAELHERGHAREDIAAQLDRGHPHIREFREAAVLVAV
                     TEREAPGVLMIHRPSNMRAHPGQIAFPGGKIDEGESPVEAALREANEELGIDPADVRI
                     VGQSDRFHTGTGYVITPVIGIVPPDLELAPNPAEVAQWFEAPLDHIFNPANQKRGTRQ
                     MNGRTMGFWEIQWEDHRIWGVTGALVVNLSRRLGWHD"
     gene            complement(4176..4724)
                     /locus_tag="VN627_05405"
     CDS             complement(4176..4724)
                     /locus_tag="VN627_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HXD02727.1"
                     /translation="MPYEPPPELAALSLAEIAAQVAARRLPPLSQWAPAVTGESHMEI
                     RADGRWYHEGGEITRPAMVRAFSGLLMRDDGGRHWLMTPHQQLTIEVEDAAFVATDVV
                     RRDDALVFRLNTDDIVLAGPEHPLRAAGDAEVPALYLAVRNGCEARLNRSTYAQLAEL
                     ALAEGTDWTVGSQGERYSLVPA"
     gene            complement(4792..>5197)
                     /locus_tag="VN627_05410"
     CDS             complement(4792..>5197)
                     /locus_tag="VN627_05410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXD02728.1"
                     /translation="VRAGTEWLQGLSFAALDENEMLAGTIQCWPVALTDPDGRAHPMI
                     MVGPVAVLPEQQSQGYGKALMTAILAAIDPRAPLPQVMIGDPEYYGRWGFTAEGTQGW
                     SLPGPYEKHRLLVRTANRSILPEEGTLGPWLR"
BASE COUNT          797 a         1819 c         1757 g          824 t
ORIGIN      
        1 ccgaggcgca gatcgtcgcc gtcatggttc accgccccgg cgcgcagttg ctgctcgcca
       61 gcaatatcgg gcgcgggttc gtcgccgagt ccggcgagct gctggccgaa acccgcaagg
      121 gcaggggggt gatgagcacc aagcccggcg tccgcctcgc cctggtgcgc gagattccgg
      181 aaagccacga tcacgtcgcc gtggtgggcg aaaaccgcaa gctcgtcgtc ttcagtctct
      241 cggaaatgcc ggtcctcgcc aaggggcagg gcgtcaccct ccagcgctac cgcgatggcg
      301 gcatggccga tgccgttacc ttcaagctcg acgagggcct gtcgtggaag atgggcggag
      361 agagcggccg cacacggacc gaaggcgaca tgcgcctgtg gaaggtggcg cgcggggccg
      421 ccgggcgaat gccgcccaac ggcttcccgc gcgacaacaa gttctaggcc ttacggctgg
      481 gctgcgggcg tcgaggccga tgcctgctgc tgcttcgcca gcgccgcctg gatctgcgcc
      541 gccgtcagct tgagcgccac gccttgggcg tccgtggtga acatgtcctt cttgagcgcg
      601 aaggagccgc cctcgcgctc aacggtcacg atgccttcgg gactgacgct ggagatcttg
      661 cccatcgccg ccccatcggc gctgtgcagc gccgcgccgg cgaccagcgc ctggtcgaga
      721 ttgcccgccg ccttggccgt cgccgcctcg accgcggcat cgagctgctc acgggtcatc
      781 gacacaagca ggcccttctc gttcttgccg aaggcgctga ccgggatcgc ggccgaatgg
      841 ctgccggtgt tgatcacggc gttgggccca tcgaccttct cgatcttgcc cacttcagcc
      901 ccctgcgggc cgtagactgt cgcgcccgcc gtcacctgga cggcagcgct cggcgcggct
      961 tgggcaaagg cgggggcggt cagcagtgca ggagccccgg tcatggcgag aactccaagt
     1021 aaaagcttga atttcataga tatgctccgg gcgggaaatc cgccatgcgc tcactggaac
     1081 gctttgcgga tgaagcgcgg atgaagctgc cgaccagccg tgctcagctt tcggcgggcg
     1141 gtttgccacc ctgggggtga tgcggcacga ggatcatcgt gctcccttcc acccgccatg
     1201 cggccagccg cgagaggaaa ttcatcccca gcacgctcgt cccttccatc ccgggcgcca
     1261 ccaccgcatc gagatcgcgc gcgacgacat tccccagcgt cagctcgttg atggtgacca
     1321 tccgcgcggg cactgtcccg ttcgcggtgc gcagcaggat cggctggcgc aggggctgcg
     1381 gctcgagccg ggccgcctcg gcaatttccg ggctgatcgc ggtcagggtc gcgccggtgt
     1441 ccaccaggaa ccggtgcggt accccgttca cgctcgccct gatccagaaa tggccgtcgc
     1501 gggccagcgg cacccgcgtc tcgctgccgc tcacttcctg cgtgggcatg cccagttgcg
     1561 gcagcgaaag gtcggccccc tggctgacgc ggatgacctg catgatcgtc agcatcaagg
     1621 ccccgaacag cccgatatgg ccaagcccgc gcaccaggcc gccgagcagg ggcgacaggt
     1681 ggcgcagcag cccgcccgcg agcaccacgg ccatggaaac cagcgccagc gtgagcagcg
     1741 gctgcgaaag caggtattgg acgagggggg ccggctccat cacgttgctt tctggcagct
     1801 tcatgcttgc ggcaagctga ccgatcaggg agcgagcgcc tcgcccagca gttcctggac
     1861 gcgctgcgca ccggggaagc cctcggccac ccaccgcgcc tcgaccgcgc gcagcacccg
     1921 tgccacttcc ggcccggcgc ctacgccccg cgccacgata tcggcaccct tgatcggcag
     1981 gcgcggaatc tcccagccgc tcagcgcagc aatatcctgc ccgtccagca aaagccggtc
     2041 gattgcctcc tcccggccca gccgataggc cagcgcgcgg gctccgtcgg cgttgccttg
     2101 ccgtccggcc gcggtaacga gccgcttgcg ctgggcggtt gacaagcgca gccgcgcgcc
     2161 gacctgttcc gccaccgccg gatcggcggg aagcaatgcg gccagccggc gcagcggatc
     2221 ggcggcagcc cccgtgcggc gttccgcggc gaccagcgcg gcaagtccgg cgggatcgcc
     2281 ttcgggcagg atcaccgtca ggatgccaag ctgtgccatc cgcgaaacgg tcggcgccgg
     2341 gtctggcagc gcaagcaggt tgagcagctc catcgcgatc cgctcgcgcg agaggccctt
     2401 gagcgtggcc gcaagctccg tgcaggcgct ttccgattcg ggatcagcag gttcggagcc
     2461 gaacctcgcc tgaaaccgga agtaacgcag gatccgcaag tgatcctcgc ggatgcgctg
     2521 gcgggcatcg ccaatgaacc gcacgcgccg gtgggcaaga tcgtccagcc cgccgaacca
     2581 gtcgaacacc tcgcgcgtga gcggatcggc atagagcgcg ttgatggtga aatcgcgccg
     2641 ggcggcatcg tcctgccaat cctgcgcata ggcaatcgtc gcccggcgcc cgtcggtgct
     2701 gacgtcatgg cgcagggtgg tgatttccac cggcccctcg ggcaggatcg ccgtcaccgt
     2761 gccatgggcg atcccggtcg gcaccgtgcg gatgccggcc gccgccagcc gctcgatcac
     2821 cgccccgggc gccagcggcg tcgccatgtc gatatccttc accgccaggc caagcagcgt
     2881 gtcgcgcacc gcgccgccga catagcggcc attgccgggg cccagcgcgg tgacaagcgc
     2941 cgccaggtcc tcgcggcctg tccacgcgtt atcgggaaga agggtggtca tcccgcgcgc
     3001 gctcccgtcc agttcagccg gcgcgcaagg ttgatcagga ccattgccgt cgatccccag
     3061 atgcaatggc cgttccagtg aatggtccac acgtgaacgg ttttttctcc gaacacataa
     3121 gggtcctcca catggttggc gggatcgagc acgtggcgta gcggcgcctc gaaccagtcg
     3181 gagacctcgg ccgggttggg ggtaatggta atgtcgccgg gcaccagcgc cagcaccgga
     3241 ttgatctggt acatcgtgcc cgtgcgcagc atgtcgccgg ggccgatcac ccggacggcg
     3301 gcggggtcga tccccatctc ctcgtgggcc tcgcgcacgg cggcctcgat gaacgtctct
     3361 ccgggatcaa ccttgccgcc cggcagcacc acctctccgg cgtgcgagcg catcgtcttc
     3421 ggccggtaga gcaggagcac gccgggatcg ggccggtcgg tgatcgccgc caggactgcc
     3481 gaagggcgat agtaaccctc cgcctggctc catgggttga cgatcacgtc aatgtcgctg
     3541 cgcgcgtggc ccgcctcgta cagccgtgtc agccggtcgt gcagttcagt catgccagcc
     3601 cagcctgcgc gagaggttga cgaccagcgc cccggtcacg ccccagatcc ggtggtcctc
     3661 ccactggatc tcccagaagc ccatggtcct gccgttcatc tggcgcgtcc cgcgcttctg
     3721 gttggcagga ttgaagatat gatcgagcgg cgcctcgaac cactgggcta cctcggccgg
     3781 gttgggggca agctcaaggt caggcggcac aatgccgata accggcgtga tcacgtagcc
     3841 ggtgccggta tgaaaccggt cgctctggcc cacgatccgc acatcggccg gatcgatgcc
     3901 cagttcctcg ttggcttcgc gcagggccgc ttcgaccggg ctttcgccct cgtcgatctt
     3961 gccgccggga aaggcaatct gaccgggatg ggcccgcatg ttcgacgggc ggtggatcat
     4021 cagtacgccg ggcgcctccc gctcggtcac ggcgaccagc acggcggcct cccggaactc
     4081 gcggatgtgg gggtggccgc gatcgagctg cgccgcaata tcctcgcggg cgtggccgcg
     4141 ttcgtgcagt tcggcgatgc gggtgaacag cgcgctcatg ccggaaccag cgagtatcgc
     4201 tcgccctggc tgcctacggt ccagtccgtg ccttcggcca gggcaagttc ggccagctgg
     4261 gcataggtgc tgcggttcag ccgggcctcg cagccgttgc gcacggcaag gtacagcgcg
     4321 ggcacttccg catcgccggc cgcgcgcagg gggtgctccg gtccggccag cacgatatcg
     4381 tcggtgttga ggcggaaaac cagggcatca tcgcgccgga ccacgtccgt ggcgacaaag
     4441 gctgcatcct ccacttcgat cgtaagctgc tgatgcgggg tcattagcca gtggcgcccg
     4501 ccatcgtcgc gcatcagcag gccggagaag gcgcggacca tggccgggcg ggtgatctcg
     4561 ccgccctcgt gataccacct gccgtcggcg cggatttcca tgtggctttc gccggtcacc
     4621 gccggcgccc attggctcag cggcgggagc cgccgcgccg ccacctgcgc ggcgatttcg
     4681 gcgagcgaaa gcgcggcaag ctctggtggc ggttcatagg gcatgccagc gcgatgccat
     4741 tgcccgcgcc gccgtgcaag gggcgctccg cccgccttgt gatggttgcg ctcagcgcag
     4801 ccaggggccg agcgtgcctt cctcgggcag gatcgagcgg ttggccgtgc gcaccagcag
     4861 gcggtgcttt tcatatgggc cgggcaggga ccagccctgc gtaccctcgg cggtgaaacc
     4921 ccaccttccg taatattccg ggtcgccgat catcacctgc ggcagcggcg cgcgcgggtc
     4981 gatcgcggcg aggatcgcgg tcatcaaggc cttgccgtaa ccctggctct gctgctccgg
     5041 caggacggcc accggcccca ccatgatcat cggatgggcg cgcccgtcgg ggtcggtcag
     5101 ggctaccggc cagcactgga tcgtgccggc gagcatctcg ttctcgtcga gcgcggcaaa
     5161 ggacaggccc tgcagccatt ccgttccggc ccgcacc
//
LOCUS       DATNBV010000222         4048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3961851,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000222 DATNBV010000000
VERSION     DATNBV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3961851"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VN627_05415"
     CDS             <1..291
                     /locus_tag="VN627_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HXD02729.1"
                     /translation="VYRGGEPAAAPLPRALGNGPVDPVADPLAIEQAYAQGFKAAATT
                     GAASPAASTAPAYSTDVAARGGDRMFAASALPTGSTIRSEWTQSGQWIAQPK"
     gene            430..741
                     /locus_tag="VN627_05420"
     CDS             430..741
                     /locus_tag="VN627_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02730.1"
                     /translation="MPIHFAGSRRPAASPLARCLARPDWVAACNDNARATGGGEAVQV
                     DTPLLRATLEHFAAHGLAAAGDAFDKAHAAARAGDQPARHHWMAVCRMLDRRLPARRS
                     R"
     gene            939..1901
                     /gene="mdh"
                     /locus_tag="VN627_05425"
     CDS             939..1901
                     /gene="mdh"
                     /locus_tag="VN627_05425"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HXD02731.1"
                     /translation="MARKKIALIGAGNIGGTLAHLAAQKELGDIVLFDVVEGVPQGKA
                     LDLSQCGPVEGFDAKITGSNDYKDIAGADVIIVTAGVARKPGMSRDDLLGINLKVMKA
                     VGEGIRDNAPNAFVICITNPLDAMVWALREFSGLPHNMVVGMAGVLDSARFSHFIADE
                     FNVSVKDVNTFVLGGHGDTMVPVTRYSTVNGIPVPDLVKMGLSTQERIDAIVQRTRSG
                     GGEIVALLKTGSAFYAPATSGIAMAEAYLNDQKRILPCAAYVDGQYGVDGLYVGVPVM
                     IGAGGVEKVIEIELDEADKAGLQVSVDAVKELLVACKGIDSTLA"
     gene            1936..2826
                     /gene="sucD"
                     /locus_tag="VN627_05430"
     CDS             1936..2826
                     /gene="sucD"
                     /locus_tag="VN627_05430"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXD02732.1"
                     /translation="MSILVNKNTKVITQGMNGKTGAFHTQAALDYGTQMVAGVTPGKG
                     GTTHIGLPQYDTVAEAKAATGATASCIYVPPPGAADAILEAIDAGIELIVCITEGIPV
                     LDMVRVKRALSGTKSRLIGPNCPGVLTPDECKIGIMPANIFKKGSVGVVSRSGTLTYE
                     AVFQTTNIGLGQTTAVGIGGDPVNGTNFIDVLELFLADEATKSIIMIGEIGGSAEEEA
                     AQFLKDEAKRGRKKPMAGFIAGRTAPPGRRMGHAGAIVSGGQGGAEDKIAAMEEAGIR
                     VSASPSLLGETLAEALKERV"
     gene            3090..>4048
                     /gene="sucA"
                     /locus_tag="VN627_05435"
     CDS             3090..>4048
                     /gene="sucA"
                     /locus_tag="VN627_05435"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683098.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HXD02733.1"
                     /translation="MGQELQDFLPDMGQDGPQPGPSWARGNWPQVGGGSEDDLTQALD
                     PLALREAIRKAATTTAGPALDEAALERAAADSIRAMLLIRTYRVRGHLAANLDPLGLA
                     RQDIPADLTPEYHGFTAADLDRKVYVGNLSHMQWATVRQMVDVLRATYCGKVGFEYMH
                     IADVEERRFIQDRIERPESFIEFSTEGKKAILSAVVRGEQYEKFLGKKYVGTKRFGLD
                     GGESMIPALEAVIKYGGQRGVKEIVYGMAHRGRLNVLANVLGKPYKVIFHEFSGGSAN
                     PEDVGGSGDVKYHLGTSSDREFDGIKVHMSLMPNPSHLETVDPV"
BASE COUNT          719 a         1364 c         1324 g          641 t
ORIGIN      
        1 gtctatcggg gcggtgagcc cgctgcggcc ccgctgccgc gcgcgctcgg caatggtcca
       61 gttgatccgg tcgcggatcc gctcgcgatc gagcaggcct atgcgcaggg gttcaaggca
      121 gcggccacga ccggtgcggc gagccccgcg gccagcacgg caccagccta ttcgacagac
      181 gttgccgcgc gcggcgggga ccggatgttc gcggccagcg ccttgccgac cggatcgacg
      241 atcaggtcgg aatggacgca atcggggcag tggatcgccc agccgaagtg aacccgcttt
      301 tccgcgccgt tggtcgggcg ggatgagaaa gccatcagtt aagcttcgga caaccatcct
      361 gcggaaccaa gccttagcgg cggtcgcggc ataggggagt ggtcagctcc cgcagaggaa
      421 atcgcagaaa tgcccatcca tttcgccggt tcgcggcgcc cggccgcctc gccgctcgcc
      481 cggtgcctgg cacggcctga ctgggttgcc gcctgtaacg acaacgcgag ggctactggc
      541 ggcggcgaag ccgtgcaggt ggatacgccg ttgcttcgcg cgaccctgga acatttcgcc
      601 gcacatggcc ttgccgccgc gggcgatgcc ttcgacaagg cccatgccgc cgctcgcgcg
      661 ggggaccagc ccgcccggca ccactggatg gcggtttgcc gcatgctcga ccggcggctt
      721 ccggcacgcc gctcgcgttg atgcaaaggt cgcatccgcg ccatattgct attgcgaact
      781 gttatcacaa ttagctgcga attgcgcatt ttacccggtt gccaagctgc cttcgcgcgc
      841 ctagggcact cgcgttcatc tgggcccctg cccgctcgcg cgggacctgg gcaggttagc
      901 ccgacagcga gatccgcccg cgtcacggaa ggtagttcat ggctcgcaag aagatcgccc
      961 tcattggcgc cggcaatatc ggcggcaccc tggctcacct cgctgcccag aaggaactcg
     1021 gcgatatcgt cctgttcgac gttgtggaag gcgtgcccca gggcaaggcg ctggacctgt
     1081 cgcagtgcgg cccggtggaa ggcttcgacg ccaagatcac cggctccaac gattacaagg
     1141 acatcgccgg cgccgacgtg atcatcgtca ccgccggtgt cgcccgcaag ccgggcatga
     1201 gccgcgatga cctgctcggc atcaacctca aggtgatgaa ggccgtgggc gaaggcatcc
     1261 gcgacaatgc gccgaacgcc ttcgtgatct gcatcaccaa cccgctcgac gcgatggtct
     1321 gggcgctgcg cgaattctca ggcctgccgc acaacatggt ggtcggcatg gccggcgtgc
     1381 tcgattcggc gcgcttcagc cacttcatcg ccgacgagtt caacgtgtcg gtcaaggacg
     1441 tgaacacctt cgtgctcggc ggccacggcg acaccatggt tccggtcacc cgttactcca
     1501 ccgtcaacgg catccccgtt cccgacctcg tcaagatggg cctctccacc caggagcgga
     1561 tcgacgccat cgtgcagcgc acccgttcgg gcggcggcga gatcgtggcc ctgctcaaga
     1621 ccggttcggc cttctacgcc ccggccacct cgggcatcgc catggccgag gcctacctca
     1681 acgaccagaa gcgcatcctg ccctgcgccg cctatgtcga cggccagtat ggcgttgacg
     1741 ggctctatgt cggcgtcccg gtcatgatcg gcgccggcgg cgtggagaag gtcatcgaga
     1801 tcgagctgga cgaggctgac aaggccgggc tccaggtttc ggtcgacgcg gtcaaggaac
     1861 tgctggtcgc ctgcaagggc atcgatagca cgctggcctg accggctccg gccttcctcc
     1921 ccaggagaga tttgaatgtc cattctcgtc aacaagaaca ccaaggtcat cacccagggg
     1981 atgaacggga agaccggcgc cttccacacc caagcggcgc tggactacgg cacccagatg
     2041 gttgccggcg tgacgccggg caagggcggc acgacccaca tcggcctgcc gcagtatgat
     2101 accgtggccg aggcaaaggc cgcgaccggc gccaccgcct cgtgcatcta cgtgccgccg
     2161 ccgggcgcgg ccgacgcgat cctcgaggcg atcgacgccg ggatcgaact gatcgtgtgc
     2221 atcaccgagg gaatcccggt gctcgacatg gtccgcgtca agcgcgcgct ttcgggcacc
     2281 aagagccgcc tgatcggccc gaactgcccc ggtgtgctga cgcctgacga atgcaagatc
     2341 ggcatcatgc cggccaacat cttcaagaag ggttcggtcg gcgtggtctc gcgctcgggc
     2401 acgctcacct atgaagccgt gttccagacc accaacatcg gcctgggcca gaccaccgcg
     2461 gtcggcattg gcggcgaccc ggtgaacggc acgaacttca tcgacgtcct cgagctgttc
     2521 ctggccgacg aggcgaccaa gtcgatcatc atgatcggcg agatcggcgg ttcggccgaa
     2581 gaagaggccg cgcagttcct caaggacgag gccaagcgtg gccgcaagaa gccgatggcc
     2641 ggcttcattg ccggccgcac ggcgcctccg ggccgccgca tgggccatgc cggggcgatc
     2701 gtctcgggcg gccagggcgg cgcggaagac aagatcgcag cgatggagga ggcgggcatc
     2761 cgcgtctctg ccagcccgtc actgctcggc gagaccctcg ccgaagcgct gaaggaacgc
     2821 gtctgaccct tcgctcgttc gcctcgtgcc cgaacagcca tgttccgggc ccgggcgcgg
     2881 cgggcgacga ggcggaacaa gaagaaatac gaaggctccg gcagggtgga cctaaccatc
     2941 ccggggccag gttggggaga aaccgggagg ctcgttgcct cctggcgtgc atgtcgtgcg
     3001 tcggcagtcc cttttgagga gcttgagggg actactggcg cacccggttt caccgaatgc
     3061 ccgcgccatg cgggtaaagg atgattgcga tgggccagga attgcaggac tttcttcccg
     3121 acatgggtca ggacgggcca cagcccggac ccagctgggc gcgcggcaac tggccgcagg
     3181 tcggcggcgg gtccgaggac gacctcaccc aggcgcttga cccgctcgcg ctgcgcgagg
     3241 cgatccgcaa ggcggccacg acgaccgccg gtcccgcgct ggacgaggcc gcgctcgagc
     3301 gcgcggcggc agattccatc cgcgccatgc tgctgatccg cacctaccgc gtgcgcggac
     3361 acctggccgc caatctcgat ccactgggcc tcgcccggca ggacattccg gccgatctta
     3421 cccccgaata ccacggcttc accgccgccg acctcgatcg caaggtttac gtcggcaacc
     3481 tcagccacat gcagtgggcc acggtgcgcc agatggtcga tgtgctgcgg gcgacctact
     3541 gcggcaaggt cggcttcgaa tacatgcata tcgccgatgt ggaggaacgc cgcttcatcc
     3601 aggaccggat cgagcggccc gagagcttca tcgagttctc caccgaaggc aagaaggcaa
     3661 tcctttccgc cgttgtccgc ggcgaacagt acgagaagtt cctcggcaag aaatacgtcg
     3721 gcaccaagcg cttcgggctg gatggcggcg aatccatgat cccggcgctt gaggcggtga
     3781 tcaagtatgg cggccagcgc ggggtcaagg agatcgtcta cggcatggcc catcgcggcc
     3841 ggctcaacgt actcgcaaac gtgctcggca agccctacaa ggtgatcttc cacgaattct
     3901 ccggcggcag cgccaatccg gaggacgtgg gcggctcggg cgacgtgaaa taccatctcg
     3961 gcacctcgtc ggaccgcgag ttcgacggga tcaaggtcca catgagcctg atgcccaatc
     4021 cgagccacct cgaaacggtc gaccccgt
//
LOCUS       DATNBV010000223         5192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3326851,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000223 DATNBV010000000
VERSION     DATNBV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3326851"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VN627_05440"
     CDS             complement(<1..774)
                     /locus_tag="VN627_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacid CoA transferase"
                     /protein_id="HXD02734.1"
                     /translation="MTDYSLAELVIVACAQAWKDDGEVMATGIGPLPRLGTALAKVQY
                     NPALQVTDGECYYGDEPVPPGKRAGPVVYEGYANYDRVFTALWGGRRHAMVGPVQFDR
                     YGQANISVIGDHQKPKSAMLGARGFPGNSITHPNSFFFPNHNTRALIAGEVDFVCMAG
                     YNPARYLNGKPPKGLDLRLIVTNLCVMDFGGPDHQIRVVSLHPGVTFEEVQENTGFPL
                     ARIAEVIPATPAPTAEQLALIARLDPNNVRATVFKDNPPG"
     gene            complement(787..1707)
                     /locus_tag="VN627_05445"
     CDS             complement(787..1707)
                     /locus_tag="VN627_05445"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXD02735.1"
                     /translation="MSQSPQSPQAPKPLDKRMSAADVVAQLRDGMTIGIGGWATRRKP
                     MALVREILKSDLKDLTVVSYGGPDVGMLCAAGKVKKLVFAFVSLDHYPLEPHFRAARQ
                     AGAIEVLEVDEGLFHWGLRAAAMDLPFLPTRIGIGTDLLTQKGFEFKMVKSPYADGEE
                     LVAMPAIRLDVALIHAQQSDEKGNLLVTSPDPLFDELFARAAPVCYASVDRIVTTAEL
                     DIEKNARFTLVERARITGVVHAPYGAHPTSSAPEYHLDLKHLKTYVDSAASPGAWAEY
                     RKSFVDVPAQDYLANVGGADVIDALPRPVY"
     gene            complement(1704..2627)
                     /locus_tag="VN627_05450"
     CDS             complement(1704..2627)
                     /locus_tag="VN627_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXD02736.1"
                     /translation="MGIRALGYVRIQTAKPEAWDHFMTQVVGVMRGDPRREDVAAYRI
                     DDRPFRFWVERGSGDRLLAAGYEVADAAALAALKETIVGLGREVVDGDGQGAKERGVS
                     AFFSTSDPSGNGLEFFWGDTRDDVAFQSEVGVSGFVTGDMGMGHAVFGTPNFDEAHAF
                     YEAIGFRDTDFPRFHFSDDPNDPGMGFAFMHADNGRHHSIAIAEMPVPPSGCVHIMLE
                     MKTQSDVGKCHDRMRLNNVPESATLGRHVNDQTFGFYMQTPSGFDIEIGADPLVIDHT
                     NWTTTAHLVPSEWGHVWAWQKALQEQQGEQA"
     gene            complement(2633..3397)
                     /locus_tag="VN627_05455"
     CDS             complement(2633..3397)
                     /locus_tag="VN627_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD02737.1"
                     /translation="MKGIDHIDLTGQVAIVTGGTRGLGRQIAQVLHSAGAAVTVCGRT
                     EPADLPEGITFRAADIRDPEQAQGLVDAVAQAHGRLDILVNNAGGSPQAPATASPRFF
                     DAIVRLNLHSAMYMSQYAHPHMAGAGGGSIVNIASVSGIRPSPMTAAYGAAKAGLLNL
                     TQSLAQEWGPDGIRVNAIIAGLVQTGAADEPYGDPATQAQIARSLPLQRLGTGVDVAG
                     TVLWLSSSLAAWVSGARINVDGGGERPYFLDLVKGS"
     gene            3643..5097
                     /locus_tag="VN627_05460"
     CDS             3643..5097
                     /locus_tag="VN627_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXD02738.1"
                     /translation="MTGYSPFRPRPVGEVPQWDFETDVAIIGFGATGACAAIEACTAG
                     AEVMLFERNSGSGGASALSGGEIYVGGGTDAQLAAGFQDTAEDLTAYLKLAGGPCADD
                     AKCELYGREALAHYQWLKDQGVPYRGNYLPGKVIEPTDDSTLIWSGSEAAAPFCHLAR
                     PAPRGHVIRHMGWGGGRPLVDILERRARDLGADIRVDARAVALVVDGDRVTGAVVRID
                     NADRFVRARRGVVLATGGFVFNEAMRRKYCPDTFRINSPIGDKDDGVGIELGVSAGGD
                     AIHMDQFFTTCPWTIPAEQAHGVFVNVAGQRFINEDCYHGRVSRCAIDQPGGKVYLLL
                     DSAHFSQPLDLAGITIAGTGETWEEVEGELGMAAGTLSATMHFYNAHAREGRDPLFDK
                     QAPILTPLDRSPFVALECNFETSYFSFFTLGGLRTSTDGEVLDRSGSAIPGLFAAGRC
                     TSGLPAWGHGYSSGLSLADCTFFGRQAGRRAALV"
BASE COUNT          869 a         1731 c         1704 g          888 t
ORIGIN      
        1 gcccggagga ttgtccttga acacagtggc ccggacgttg ttcgggtcaa gccgggcgat
       61 cagcgccagc tgctccgccg tcggcgccgg agtggcgggg atcacttccg cgatcctggc
      121 gagcggaaaa ccggtgttct cctggacttc ctcgaaggtc acgcccgggt ggagcgagac
      181 cacccggatc tgatgatccg gaccgccgaa gtccatcacg cacaggttgg tgacgatcag
      241 gcgcaggtcc aggcccttgg gcggcttgcc gttcaggtag cgggcgggat tgtagcccgc
      301 catgcacacg aaatcgacct cgcccgcgat gagcgcgcgg gtattgtggt tcgggaagaa
      361 gaagctgttg ggatgggtga tggagttgcc cgggaaaccg cgcgccccca gcatcgccga
      421 cttgggcttc tggtgatcgc cgatgacgct gatgttggcc tggccgtagc ggtcgaactg
      481 gactggcccc accatggcat gacgccggcc gccccacagc gcggtaaaca cacggtcgta
      541 gttggcatag ccttcgtaga ccaccggtcc ggcgcgcttg cccggcggca cgggctcatc
      601 cccgtaatag cattcgccat cggtaacctg cagggcggga ttgtactgga ccttggccag
      661 agcggtacca agccgcggca gcggcccgat gccggtggcc atgacttcgc cgtcatcctt
      721 ccaggcctgg gcacaggcaa cgatgacgag ctcggcgagc gaatagtcgg tcacgattgc
      781 ctctcctcag tagaccgggc gcggcagcgc atcgatgacg tcggcgccgc cgacattggc
      841 aaggtagtcc tgcgccggaa catcgacgaa cgacttgcgg tactctgccc aggccccggg
      901 cgaagcggcg ctgtcgacgt aggtcttcag gtgcttgaga tcgaggtggt actcgggcgc
      961 cgagctggtc gggtgggcgc catagggcgc gtggaccacg ccggtgatcc gtgcgcgttc
     1021 caccagcgtg aagcgggcgt tcttctcgat atcgagttcg gcggtcgtca cgatccggtc
     1081 gacgctggca tagcagaccg gcgcggcgcg ggcgaacagc tcatcaaaca gcggatcggg
     1141 cgaggtcacc aggagattgc ccttctcgtc cgactgctgg gcgtggatca gcgccacgtc
     1201 gagcctgatc gccggcatgg ccaccagctc ctcgccatcg gcataggggc tcttgaccat
     1261 cttgaactca aaccccttct gggtgagcag gtcagtcccg atgccgatcc gggtcggcag
     1321 gaacggcagg tccatagccg cggcgcgcag gccccagtgg aacaggccct cgtccacctc
     1381 cagaacctcg atcgcgccgg cctgtcgcgc ggcgcggaaa tgcggttcga gcggatagtg
     1441 atcaaggctg acaaaggcga agaccagctt cttgaccttg cctgccgcac acagcatgcc
     1501 gacatccggc ccgccatagg acaccacggt caggtccttg agatcggact tcaggatctc
     1561 gcgcaccagc gccatcggct tgcgccgggt cgcccagccg ccaatgccga tggtcatgcc
     1621 atccctgagc tgcgcgacga catcggccgc gctcatccgc ttgtcgagcg gcttcggagc
     1681 ctggggactc tgggggctct ggctcacgcc tgttctcctt gttgttcctg cagcgccttc
     1741 tgccaggccc agacgtggcc ccactcgctt ggcacgaggt gggcggtagt ggtccagttc
     1801 gtgtgatcga tgaccagcgg atcggcgcca atctcgatgt cgaagcccga gggcgtctgc
     1861 atgtagaagc cgaaggtctg gtcgttgacg tggcgaccca gcgtcgcgct ctcgggcaca
     1921 ttgttgaggc gcatacggtc atggcacttg cccacatcgc tctgggtctt catctccagc
     1981 atgatgtgga cgcagcccga gggcggcacc ggcatttccg cgatggcgat cgaatggtgg
     2041 cggccattgt ccgcgtgcat gaacgcgaag cccatccccg ggtcgttcgg atcgtcggaa
     2101 aagtggaagc gcgggaagtc ggtgtcgcgg aagccgatcg cctcgtagaa ggcgtgggcc
     2161 tcatcgaaat tgggcgtgcc gaacacggcg tggcccatgc ccatgtcacc ggtaacgaag
     2221 ccgctgacgc caacctctga ctggaacgcc acgtcgtcgc gggtatcgcc ccagaagaat
     2281 tcgaggccgt tgcctgacgg gtcgctggtg gagaagaacg cggaaacgcc gcgctctttc
     2341 gccccctgcc cgtcaccgtc gacgacctct cggccgagtc caacgatggt ctccttcagg
     2401 gccgccagtg ccgccgcgtc ggccacctcg tagccggcgg caagcagccg gtcgccggag
     2461 ccacgctcca cccagaagcg gaacgggcga tcgtcgatcc ggtaggccgc gacgtcttca
     2521 cgccggggat caccgcgcat cacgccgacg acctgcgtca tgaagtgatc ccaggcctca
     2581 ggcttggcgg tttggatcct gacatagccc agcgcacgaa tgcccatgcc cctcaacttc
     2641 ctttcacgag gtcgaggaaa tagggccgct cgccccctcc atcgacattg atacgtgccc
     2701 cgctgaccca tgcagccagg cttgaactca gccacaggac ggtgccggcg acatccacgc
     2761 cggtccccag gcgctgcagc ggcaaggacc tggcgatctg ggcttgtgtt gcggggtccc
     2821 cgtagggttc gtccgccgcc ccggtctgca ccaggccggc gatgatcgcg ttcacgcgga
     2881 tgccgtccgg cccccattcc tgggcaaggc tctgggtaag gttcagcagc ccggccttgg
     2941 ccgcaccgta ggcggcggtc atgggcgatg gccggatgcc ggagacgctc gcgatgttga
     3001 cgatgctccc gccgcccgcg cccgccatgt ggggatgggc atattgcgac atgtacatcg
     3061 ccgaatgtag gttgagcctg acgatggcat cgaagaaacg cgggctggcc gtggccggcg
     3121 cctggggcga accgccggca ttgttgacga ggatgtcgag ccggccgtgc gcctgcgcga
     3181 ccgcgtcgac cagcccctgc gcctgctccg ggtcgcggat atccgccgca cgaaaggtta
     3241 tgccctcggg caggtcggcc ggctcggtcc ggccgcatac ggttacggcg gcgccggcgc
     3301 tgtgcagcac ctgcgcgatc tgccgcccga gaccgcgcgt gccgcccgtg acgattgcga
     3361 cctgtccggt cagatcaatg tgatcgatgc ccttcaccgg gctgctctcc cttgctctga
     3421 cgcctgcctg gacgtcgccg gcgaaatatt acccacgcga ttccaaaaac gccaatatcg
     3481 aaagtctata ggcttttcaa ttgacgattt gcgtacccaa tgcaattata aattacgaat
     3541 gcggcgcaac aacgcgtgcg aacccttgtc ctgccatgcc ggcagactgt gcggtcgcga
     3601 gcgaaccgac ccgcgcatcg cgcttgggag catgtggacg ggatgaccgg atactcacca
     3661 ttccgccccc ggcccgtggg ggaggtcccc cagtgggact tcgagacgga cgtggctatc
     3721 atcggattcg gcgcgaccgg cgcctgcgcc gcgatcgagg cttgcaccgc cggtgcagaa
     3781 gtcatgctgt tcgagcgcaa ctcgggcagc ggcggcgcat cggccctctc cgggggggaa
     3841 atctacgtgg gcggcgggac cgacgcccag ctggccgcgg ggttccagga cacggccgaa
     3901 gaccttaccg cctatctgaa gctcgccggc ggcccctgtg ccgacgatgc caaatgcgaa
     3961 ctctacggcc gcgaggcgct ggcgcactac cagtggctga aggaccaggg cgtaccctat
     4021 cgcggcaatt atctgcccgg caaggtgatc gagccgaccg acgattccac cctgatctgg
     4081 tcgggcagcg aagcggcggc tccgttctgc cacctggccc gccccgcgcc gcgcggtcat
     4141 gtgatccggc acatgggctg gggcggtggc cgcccgctgg tcgacatcct ggaacggcgc
     4201 gcccgcgatc tcggcgccga catccgcgtg gacgcccgcg ccgtcgccct ggtggtggac
     4261 ggagaccgcg tgacgggcgc cgtggtacgg atcgacaatg ctgaccgctt cgtccgcgcc
     4321 cgccgcggcg tcgtgctggc caccggcggc ttcgtgttca acgaggcgat gcgccgcaag
     4381 tattgcccgg acacgttccg gatcaacagc ccgatcggcg acaaggacga cggcgtgggc
     4441 atcgaactgg gcgtttcggc cggcggcgat gcgatccaca tggaccagtt cttcaccacc
     4501 tgcccgtgga cgatcccggc cgaacaggcc cacggggtct tcgtcaacgt tgcggggcag
     4561 cgcttcatca atgaagactg ctaccacggc cgggtcagcc gctgcgcgat cgaccagccc
     4621 ggcggcaagg tctatctgct gctcgacagc gcccatttct cccagccgct ggacctcgcc
     4681 ggaatcacca tcgccggcac cggggaaacc tgggaagagg tagagggaga gcttggcatg
     4741 gcggccggca ccctctcggc cacgatgcac ttttataacg cccacgcccg ggaaggccgc
     4801 gatcccctgt tcgacaagca ggcgccgatc ctcacgccgc tcgaccgatc gccgttcgtc
     4861 gcgctcgagt gcaatttcga gacgagctat ttcagctttt tcacgctcgg cgggctcagg
     4921 acctcgaccg acggcgaagt gctggaccgg tccggttcgg ccataccggg cctgttcgcc
     4981 gcgggccgct gcacatccgg gcttcccgcc tgggggcacg gctattcctc aggcctgagc
     5041 ctggccgact gcacgttctt cgggcgtcag gccggtcggc gggcagccct ggtctgagtg
     5101 acgatcagcg ggaaacgtca ccaggccgtt cccattccac taacttgcca ttaagtcagc
     5161 acttacactt accaaggaac cccatgatgc tt
//
LOCUS       DATNBV010000224         2209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3962770,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000224 DATNBV010000000
VERSION     DATNBV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3962770"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VN627_05465"
     CDS             <1..382
                     /locus_tag="VN627_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD02739.1"
                     /translation="PRTAGAQSADAASVEQAVETLRKLMIAPDQKGLEDILADKLTYG
                     HSDGRVQNRAEFIDALMTKKSSFSAITLSDQTVSIVGDLAIVRHVFAADAVSGGKAAQ
                     PRIRILQVWQKQDGKWRLLVRQAR"
     gene            complement(407..1859)
                     /locus_tag="VN627_05470"
                     /pseudo
     CDS             complement(407..1859)
                     /locus_tag="VN627_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043743199.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
     gene            complement(1799..>2209)
                     /locus_tag="VN627_05475"
     CDS             complement(1799..>2209)
                     /locus_tag="VN627_05475"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HXD02740.1"
                     /translation="GAGLAVRLRVPAGALLVPMIAAALLQGAGWLTVTVPRAVLMIGY
                     GAIGLSIGLRFRRELLRRTLRAIPEMLASAVALIGLCGVSAWVLILLSQKIAVRMRCE
                     DGREEIRCAADGWWQWRSCPVGAVKRVLRPMSRS"
BASE COUNT          349 a          803 c          735 g          322 t
ORIGIN      
        1 gccgcggacg gccggcgcgc aatccgccga cgcggcgtcc gtcgagcagg cggtcgagac
       61 cctgcgcaag ctgatgatcg ctccggacca gaaggggctg gaggacatcc ttgccgacaa
      121 gctcacctac ggccattcgg acgggcgcgt gcagaaccgg gccgagttca tcgatgcgct
      181 gatgacgaag aagtcgtcgt tcagcgcgat cacgctctcg gatcagacgg tctcgatcgt
      241 cggcgacctc gcgatcgtgc gccacgtgtt cgcggccgac gccgtgagcg ggggcaaggc
      301 cgcgcagccg cgcatccgca tcctgcaggt gtggcagaag caggacggca agtggcggct
      361 gctcgtgcgg caggcgcgtt gagccggaac tggcgtggtc ctagtactac tgcgtcacaa
      421 gcggcgatgg aagacgggtc ggctgtgatt gctctgattt gtgcgtgcgc agcacgggca
      481 tcgatgcagg ctccggacca gcgccacggt gagccgtcgt cgcacggtgg cgatcgagtt
      541 cggcacgtgc cgttcgggtc ggatcggcgg cgccgcgggg tcggtaaccc tcggaaaggc
      601 gaggcgcttc gaaacccccg gcgcgacttg ggcctgaggg gggaaaatct cgcccgctcg
      661 gcgatcagaa agccataagc cgcgatgcac agcgtggcgt gatggtggaa gccgcgccag
      721 ccgcggccct cgtagtgccc gagcccgact tcctgcttga gatcctggta atcccgctcg
      781 atgcgccagc gcagctgagc caagtcgacc agatcggcga agccggtctc ctcgggcagg
      841 gtcgacagcc agtactgcgt cggctccggc tcgccctcgg gccactcgat cagcagccat
      901 tcctcctcac gcggcgcgct cagccgggtg tcggcgtgcg ccgcgcgcac ccgggtccgg
      961 gcaaagcgcg aggcaagctc cccgttcgtt ccttcgcgcc aagcgatctc ggtccacgcc
     1021 gtctcgggca gcgccagcgc caccgccttg accgggaccg gctggtgctg cgcgtcgcgg
     1081 cgcagccgcg tcgctcgccg cccccggccc gaccagggct tgggcggcag cggtcccgtg
     1141 cccggcggcc acgccgtcgt gctcgacaga acagccgcca cataggacag accgagcgcg
     1201 ctcacgcccg tgcggaagcc cgtgtccttg ccgtaacccg catccgtcac caccagcgca
     1261 ggcggcaggc ccgccgcggc cgccgcccgg agcagatcga gcgcgatctc tggcttggtc
     1321 cggaaggcga tctcctcggg cacccccgcc ttggcgcgcc gcgccggatc ctcggcccag
     1381 tcccggggca gatacaggcg ataggacacc ggcaggctgg cggcgtggtt ggcgagcgac
     1441 aggctgaccg cgacttggca gttgtcctgc ttgccgagct ggccgcaata ctgccgcccc
     1501 acccccactg agtgccgccc cttcttgggg aagctcgtgt cgtcgatgat ccaggcggca
     1561 atcggctccg tgcgcgtcat ctccggcagg acgatctcgc gcaccttggc cagcatcgcc
     1621 gcatccgacc agggcgcgtt cgccacgaag tgcagcaagg actggtgctg agccgccacc
     1681 cgcgccggcg cggtcacggc cgccatcggc tcgacgctct tcctctcggc cggcatcaac
     1741 aggcccgtgc agtagtcgcg tagcggtccg gctcgatcgg catggccgat caccccggtc
     1801 agcttctcga cataggccgc aaaacgcgct ttactgctcc gaccggacag cttcgccatt
     1861 gccaccatcc atcagcagcg caccgaatct cctccctacc atcttcgcaa cgcatccgga
     1921 ccgcaatctt ttgactcagt agtattagga cccacgccga taccccgcac aggccgatca
     1981 gcgcgacggc cgaggcgagc atctcgggga tcgcccgcag cgtccggcgc aggagctcgc
     2041 ggcggaacct gaggccgatc gacaggccga tcgccccgta gccgatcatc agcacggctc
     2101 tcggcaccgt aaccgtgagc catccggcgc cctgcagcag ggccgccgcg atcatcggca
     2161 ccagcagcgc accggccggg accctgaggc gcaccgccag tcccgctcc
//
LOCUS       DATNBV010000225         2632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2353235,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000225 DATNBV010000000
VERSION     DATNBV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2353235"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VN627_05480"
     CDS             <1..270
                     /locus_tag="VN627_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02741.1"
                     /translation="IPAALLAQIADLFEVPLVWFFQGIGEDDSHDDGTDREDDGERKA
                     GPAIYQVATIEHRIAALVESFRKLDFEGQQHLLAISRELSRAARE"
     gene            complement(353..928)
                     /locus_tag="VN627_05485"
     CDS             complement(353..928)
                     /locus_tag="VN627_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02742.1"
                     /translation="MPDMLNTAEPKAREIPIEPGGTGAYRRNIEICAAGKPLGRITML
                     LRVVRVLTGFALASFAAAATLVLFVYAPADTASLSTDINGARLSEAGYFALVITPWVA
                     LSAALPGLAGVFYAETQRLAGWPFYVFAGLGTAFAGFLLQHASESRGGPGIFEAYALI
                     AFLVAGIVGGLVYWAASGRYVMRAVAAAKTS"
     gene            complement(994..2199)
                     /gene="rlmN"
                     /locus_tag="VN627_05490"
     CDS             complement(994..2199)
                     /gene="rlmN"
                     /locus_tag="VN627_05490"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXD02743.1"
                     /translation="MDIAYDLRQPVPPTAAKPSLAGLGRDGLAAALAAVGVPDRQLKM
                     RVGQLWSWLYVRGARTFEEMSDVSKGLRAALAGAYTLARPEIAAAQVSADGTRKWLLR
                     LQRRGHEARAPEIETVFIPEADRGTLCISSQIGCTLTCSFCHTGTQRLVRNLTPEEIV
                     AQILIARDAAGDWPGAAPPQDGRLMPAGERKITNVVLMGMGEPLYNFDNVRDAMAVAA
                     DGDGLALSKRRITLSTSGVVPEIPRWGGEAGTMLAISLHATDDELRNKLVPINRKYPI
                     AELLAACRAYPGLSNARRITFEYVMLKGVNDSLAEAKALVRLLGGIPAKINLIPFNPW
                     PGTTYECADWEQIERFAEIVNKAGYASPVRTPRGRDIMAACGQLKSASLKLAARDRGS
                     PGSIEEAVG"
     gene            complement(2355..2582)
                     /locus_tag="VN627_05495"
     CDS             complement(2355..2582)
                     /locus_tag="VN627_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02744.1"
                     /translation="MQHNRHINSVQWAQAFGYAREACAGIFRDGGSPRAALGAFGLGT
                     AEPADWSAAVDRIALALCSRPARTWQTRKAA"
BASE COUNT          396 a          894 c          963 g          379 t
ORIGIN      
        1 atcccggccg ccctgctggc gcagatcgcc gatctgttcg aggtccccct ggtgtggttc
       61 ttccagggca tcggggagga cgactcccac gatgacggga cggaccggga ggacgatggg
      121 gagcgcaagg cggggcccgc gatctaccag gtcgccacga tcgagcaccg catcgccgcc
      181 ctggtcgagt cgttccgcaa gttagacttc gaggggcagc agcacctgct cgcgatctcc
      241 agggagctct cccgcgccgc gcgggagtga tgtcggctct cgcaggccgg gcacgctgcc
      301 gaaggcggca accccgcctg gccggggggc ggtctcgtcc gccggcgtcg agtcaggagg
      361 tcttggccgc ggcgaccgcg cgcatcacgt accgccccga ggcggcccag tagaccaagc
      421 cgccgacgat gccggcgacc aggaaggcga tgagcgcgta ggcctcgaag atgccgggac
      481 cgccgcgcga ctcggacgcg tgctgcagga ggaagccggc gaaggccgta cccaagccgg
      541 cgaacacata gaagggccag ccggcgagcc tctgggtctc ggcgtagaac acgccggcca
      601 gccccggcag cgccgccgac aaggccaccc agggggtgat caccagggcg aaatagcccg
      661 cctccgacag gcgtgcgccg ttgatgtcgg tgctcaggct cgcggtgtcg gcgggggcgt
      721 agacgaacag cacgagcgtc gccgccgcgg cgaagctcgc cagggcgaac cccgtcagta
      781 cccgtacgac acgcaggagc atggtgatcc gacccaaggg tttgccagcg gcgcagatct
      841 ctatgttgcg tctataggca cccgtgccgc cgggctcaat ggggatttcg cgggccttgg
      901 gctctgccgt gttcaacata tcgggcaagg cggcccgatt gagcggattt cccggtcaaa
      961 atcgtgtacg agcggtcgaa ccgccgtggc cgactatccg accgcttcct cgatgctgcc
     1021 cggcgacccg cggtcgcgcg cggcgagctt caagctggcc gacttgagct ggccgcaggc
     1081 ggccatgatg tcccggccgc gcggggtgcg cacggggctc gcatagcccg ctttgttgac
     1141 gatctcggcg aagcgctcga tctgctccca gtcggcgcac tcgtaagtgg tgccaggcca
     1201 ggggttgaac gggatcaggt tgatcttggc cgggatgccg cccagcagcc gcaccagggc
     1261 cttggcctca gccaggctgt cgttcacgcc cttcagcatc acgtactcga aggtgatgcg
     1321 tcgggcgttg gagaggcccg gataggcgcg gcaggccgcc agcagctcgg caatcggata
     1381 cttgcggttg atgggcacca gcttgttgcg caactcgtcg tcggtggcgt gcagcgagat
     1441 ggccagcatg gtgccggcct caccgcccca gcgcgggatc tccggcacca cgccgctggt
     1501 ggacagcgtg atgcgccgct tcgacagggc aagcccatcg ccgtcggccg cgaccgccat
     1561 ggcatcgcgc acattgtcga agttgtagag cggctcgccc atgcccatca gcacgacgtt
     1621 ggtgatcttg cgctcgcccg ccggcatgag gcggccgtcc tgcggggggg ccgcgccggg
     1681 ccagtcgccg gcggcgtcgc gggcgatcag gatctgggcg acgatctcct ccggcgtcag
     1741 gttgcgcacc aggcgctggg tgccggtgtg gcagaacgag caggtgaggg tgcagccgat
     1801 ctggctcgat atgcacagcg tgccgcggtc ggcctccggg ataaagacgg tctcgatctc
     1861 gggcgccctg gcctcgtgcc cgcgccgctg cagccgcagg agccacttgc gggtgccgtc
     1921 ggcggagacc tgggcagcgg cgatctccgg gcgcgccagg gtgtaggcgc cggccagggc
     1981 ggcgcgcagc cccttggaga cgtccgacat ctcctcgaag gtgcgggcgc cgcgcacgta
     2041 gagccagctc catagctggc ccacccgcat cttcagctgc ctgtcgggca cgccgacggc
     2101 ggcgagcgcg gctgcaagcc cgtcgcggcc gaggcctgcc agagacggct tggcggcggt
     2161 cggtggcact ggctggcgaa gatcgtaggc gatgtccatg ggatccgggc tgctcggcgc
     2221 gcgactggca gggttcgccc cctatgtagc gaacgacgcg cccgtcgtga agcccgccgc
     2281 ggcgccgcgt acggccccag ggcctccgat cgcgccgccc ggcagaccgg aaagcggcct
     2341 ggagtagagc ggtcctaggc ggccttgcgt gtctgccaag tgcgggccgg gcgggagcag
     2401 agcgcgagcg cgatgcggtc cacggcggcg ctccagtcgg ccggctcggc ggtgcccagg
     2461 ccgaaggcac cgagcgcggc gcgcgggctg ccgccgtcgc ggaaaatgcc ggcgcacgcc
     2521 tcgcgcgcgt agccgaaggc ctgcgcccac tgcaccgagt tgatgtggcg gttgtgctgc
     2581 attcgcgtcc ccgtctttca atccttcaag ccaaacggca ggccgtacaa cc
//
LOCUS       DATNBV010000226         2477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4274490,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000226 DATNBV010000000
VERSION     DATNBV010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4274490"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1596)
                     /locus_tag="VN627_05500"
     CDS             complement(82..1596)
                     /locus_tag="VN627_05500"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HXD02745.1"
                     /translation="MNKPLNAPGSAYTRLEQRFRRLSALQEAAGMLHWDMSTVMPDGG
                     RPARAEQLATLGVVCHEILTEAETGALLDQAEGEAGGLTQWQRANLGEMRRNHTHATA
                     LDSDLVEAMSKAATACEAVWREARPKGDFVLVYPLLSEVLNLSRQAAAAKAEKLGCSP
                     YEALMDQYEPGAREVDIDRVFADYSAFLPDFLPRVLEHQARRGKPIEPKGPFPQAVQK
                     DLAKRVMQGLGFDFTHGRLDESLHPFCGGVPEDVRITTRYSEDAFVQALMGVIHETGH
                     ALYERNLPAEWRLQPVGVARGMVVHESQSLLMEMQAARSAQFCRHLSGLLRETFPGND
                     AALSPDNLQRIYSRVEPGFIRVDADEVTYPAHIILRYQIEKSLFRDDLQLADVPGVWR
                     EEFKKLLGLDVPSDRQGCLQDVHWYDGAFGYFPCYSLGAMTAAQLFDAACRAHPVIPA
                     EIEQGKFATLQGWLKANVHSLGSSLTTPEIVTHATGRPLDVAVFKRHLERRYLS"
     gene            complement(1593..2315)
                     /locus_tag="VN627_05505"
     CDS             complement(1593..2315)
                     /locus_tag="VN627_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HXD02746.1"
                     /translation="MLSKLRFRPTLWPTLFSVPAIIVMIGLCVWQVQRLHWKEGLIAE
                     RESRVAAEPVALPPLGVDPASMEYRRIRLEGTFLHDKELFLGARSMNGNVGYQVLTPF
                     ALSAGGTVLVDRGWVPVERKAPESRAEGQLAGTQAVDGIVRLAHGQAWMQPDNEPARN
                     MWFFVDLPAMAAASGIDLRTDLYVDAGPAENPGKYPVGGQTRIELPNDHLQYAITWGL
                     LAAALAVIYVLYHLKLERERHT"
BASE COUNT          423 a          816 c          834 g          404 t
ORIGIN      
        1 ttgtgattca ccttctccga ccatgggcga gtcgccaagc gccctgccga tcggtcgccg
       61 agcaccggtc ggtacggacg ctcagctcaa ataccgccgc tccaggtgcc gcttgaacac
      121 ggccacgtcc agtggccggc cggtggcgtg ggtcacgatt tcgggggtgg tgaggctgga
      181 gccgaggctg tggacgttgg ccttcagcca gccttgcagg gtcgcgaact tgccctgctc
      241 gatctccgct ggaatcacag ggtgcgcccg gcaggcggcg tcgaacaatt gcgcggcggt
      301 cattgcgccc aggctgtagc aggggaaata gccgaaggcg ccgtcgtacc aatgcacgtc
      361 ctgcaggcag ccttggcggt cggacggcac gtcgaggccg agcagtttct tgaattcctc
      421 gcgccacacg cccggcacat cggcaagctg caggtcgtcg cggaacaggc tcttttcaat
      481 ctggtaacgc aggatgatgt gggcgggata ggtcacctcg tcggcatcga cgcggatgaa
      541 gcccggctcc acgcggctat agatgcgctg cagattgtcc ggcgacaagg ctgcgtcgtt
      601 gccgggaaag gtctcgcgca gcaggccgga gagatggcgg cagaattgcg cgctgcgcgc
      661 cgcctgcatc tccatcagca gagactggct ttcatgcacc accatgccgc gggcgacgcc
      721 gaccggctgc aggcgccatt cagcaggcag gttgcgctca tagagcgcgt ggccggtctc
      781 gtggatcacg cccatcagag cctggacgaa cgcatcctcg ctatagcgcg tggtgatgcg
      841 cacatcctcc ggcacgccgc cgcagaacgg atgcaggctt tcatccagcc ggccatgggt
      901 gaagtcgaaa ccgaggccct gcatcacgcg cttggcgagg tccttctgca cggcctgcgg
      961 gaacggcccc ttcggctcga tcggcttgcc ccggcgggcc tgatgctcca gcacgcgcgg
     1021 caggaagtcc ggcaggaacg cgctgtaatc ggcgaatacg cggtcgatgt ccacttcacg
     1081 cgcgcccggc tcatactggt ccatcagcgc ttcatagggg ctgcagccga gcttctccgc
     1141 cttggccgcc gccgcctgcc gcgaaaggtt cagcacttcg gacagcagcg ggtagaccag
     1201 cacgaaatcg cccttgggcc gcgcctcgcg ccacaccgct tcgcaggcgg tcgcggcctt
     1261 gctcatcgcc tccaccagat cggaatcgag cgcggtcgca tgggtgtggt tgcggcgcat
     1321 ttcaccaagg ttggcgcgct gccactgggt gagaccgccc gcctcgcctt ctgcctggtc
     1381 cagcaaggcg ccggtttccg cttcggtcag gatctcgtgg cacacgacgc cgagggtcgc
     1441 gagctgctcg gcgcgcgccg gacggccgcc atcgggcatc acggtcgaca tgtcccaatg
     1501 cagcatgccc gcggcctcct gcagggcgga gaggcggcgg aagcgctgtt ccagccgggt
     1561 ataggcgctg ccaggggcgt tcaggggctt gttcatgtgt ggcgctcgcg ctccagcttc
     1621 agatgataga gcacatagat gacggccaag gccgccgcca gcaagcccca ggtgatggcg
     1681 tattgcaaat ggtcgttggg caactcgatc cgggtctggc cgccgaccgg gtatttgccg
     1741 gggttctccg ccggcccggc atcgacatag agatcggtgc gcagatcgat tccggaagcc
     1801 gccgccatcg ccggcagatc gacgaagaac cacatgtttc gagcgggttc gttgtccggc
     1861 tgcatccagg cctggccgtg ggccagccgg acgatgccgt ccacggcctg ggtgccggcc
     1921 agctggccct ccgcgcggct ttccggagcc ttgcgctcga ccgggaccca gccgcgatcg
     1981 accaggacag tgccgccggc agagagcgcg aagggcgtga ggacctgata gccgacattg
     2041 ccgttcatcg agcgcgcgcc gaggaacagc tccttgtcat gaaggaacgt gccttcgagg
     2101 cggatgcggc gatattccat gctcgcggga tcgacgccga gaggcggcag cgccaccggc
     2161 tcggccgcca cgcgcgattc gcgctcggcg atcaggcctt ccttccagtg caggcgctgc
     2221 acctgccaga cgcagaggcc gatcatcacg atgatggcgg gcacggagaa cagggtcggc
     2281 cacaaggtcg gccggaaacg aagcttgctc aacatgcggt caggatctgt gcttgaactg
     2341 caggccgatc atgacgccct tggccgggcg cagcagccac agcgacagcc cgatgacgac
     2401 gatcggccag aggatggcat gcacccagat cggcggctcg aacgccgccc ataccacgaa
     2461 taccagcggc accgtga
//
LOCUS       DATNBV010000227         3159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_778846,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000227 DATNBV010000000
VERSION     DATNBV010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778846"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VN627_05510"
     CDS             complement(<1..443)
                     /locus_tag="VN627_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin knob domain-containing protein"
                     /protein_id="HXD02747.1"
                     /translation="MPRNPGTGIYTKPYPDVISDTTIESTVHNGEIADIETDLNTPRP
                     IVAGGTGANNATQARDNLDAEVAAQTVTNYDTHVWESGSFWSQTSATGGPAPGQVVTG
                     FAVVAAGSPDWITLLAVDHNTGNTFIRIKVAGVWGAWSQRAGSIV"
     gene            complement(457..2037)
                     /locus_tag="VN627_05515"
     CDS             complement(457..2037)
                     /locus_tag="VN627_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02748.1"
                     /translation="MPLVYDDTPSGELKYDEAPRPLSNWELTKSIGGTIGQTLIDAGA
                     AAGHAATFGLDVRRDALSGWLSGDYPSYSAGVEAEQAKLAKQRERSPYASIAGDVAGA
                     ALIPGMGGAGLAARLGTGLGRWGQAGARAAGYGLEGAALGAAQGAGTTYSGKPVDYLT
                     NAATGFGVGGVLGAGMGSVFGPRGGMRSTAEMPTTPELRTDKTARYTALENSPALYEP
                     NALARSATLTEDVLRAQRHTPERSPISYQAIDEMRAPPTTEHLGVGAPIGPGDIEYIR
                     KGLNQVNPVSGGPDIAAAPVVKRALDNFVLNPPPGAVIPGTERAAAEASALAQSARDA
                     HGAYRRSQLWDSLTENALTSAGSANSGLNVQNRLRQVVTGGLRQNDKGQSAFSKAHYS
                     PEEIARLTEFARVPRGDATLRYMDRLFGGGGGLGAIAAGGVGGGAVGQYFKDDPTWGS
                     IAGVGVPAAGLGLRLLGNRRATQQMQGLGNMVRERAPLYQERLAAAPMVPPPGGGAAA
                     SARDALTIELLRQQGLTP"
     gene            complement(2037..2699)
                     /locus_tag="VN627_05520"
     CDS             complement(2037..2699)
                     /locus_tag="VN627_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02749.1"
                     /translation="MDEKNRANLAIAAYGNLPDYVRTHLDTSKEKALAAVGAQEAIND
                     AKLAVNSGALFGFGAEAKLQAYRAAAAAGNKDAERIVAATQSYKSNLGPIMQATIKTY
                     AGKDVSKQELEFVQKMAGSDISLDPDTVMRILNIAERNAQENIRSHKGMLDSMLAGQQ
                     PSAHPALRSLYDVREPLPAVAVPSTVPAGPIVDVRSEEEANKLPAGSRFRLNGRTGTK
                     VR"
     gene            complement(2624..>3159)
                     /locus_tag="VN627_05525"
     CDS             complement(2624..>3159)
                     /locus_tag="VN627_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02750.1"
                     /translation="ATDAPSPMARGGRMAIPPLTEENPVTPSDITPAPTRLAEARGAV
                     PGAAPVLSPGAAPGGPPKAYVAEKPTPPALPERTPESPREKYARDMYLKNQNDPYSAP
                     MWKAIVDDAEAKRKYIDDRNKTIYDAQVQDWRTKETTYQQQKATEQERQQAWTRRTAP
                     TWRSQRTATCPTTCART"
BASE COUNT          474 a         1033 c         1000 g          652 t
ORIGIN      
        1 tcaacaattg atcctgccct ttgcgaccac gcgccccaca ctcctgccac ttttatgcga
       61 atgaaagtat taccggtgtt gtgatcaacc gcgagcagcg tgatccagtc tgggcttccg
      121 gcggcaacaa ccgcaaagcc agtgacgacc tgccccgggg caggtccgcc ggtagcactg
      181 gtctgcgacc agaagcttcc gctttcccag acgtgggtgt cgtagttggt gacggtctgt
      241 gccgccacct cggcatcaag gttatcacgc gcctgcgtgg cgttgttcgc gccggtcccg
      301 cccgccacga tcggccgcgg cgtgttcagg tcggtttcga tgtcggctat ctcgccattg
      361 tgcacggtac tctcgatcgt cgtgtcgctg atcacgtcag gatacggctt ggtgtaaatg
      421 ccggtgccgg gattgcgggg catggttaat ttcctcttag ggcgtcagac cttgctggcg
      481 caataactcg atcgtcagag catcgcgtgc acttgccgcg gctccgccac caggtggcgg
      541 taccatcggt gcggcagcga gccgttcttg gtaaagcggt gcacgttcgc gcaccatgtt
      601 gcccagacct tgcatttgct gagtagcgcg ccggttgccc agcaggcgca gacccaggcc
      661 ggccgccgga acgccgacgc cggcgatcga gccccatgtt ggatcgtcct tgaagtattg
      721 cccaactgcg ccgccgccaa cgccgcctgc agcgatagca ccaaggccgc cgccaccacc
      781 gaacaggcgg tccatatagc gcagcgtcgc atcgccgcgt ggaacgcgcg caaattcggt
      841 caaccgagcg atttcctccg gactgtaatg cgccttggaa aacgccgact gccctttgtc
      901 gttctggcgc agaccgccgg tcacgacctg gcgcagccgg ttctgtacgt tgagccctga
      961 gttggccgac cccgcgctgg tcaaggcgtt ctcggtaagg ctgtcccaca attggctgcg
     1021 ccggtatgcg ccgtgtgcgt cgcgggcgct ttgggccagc gcggaagcct ccgccgcggc
     1081 gcgctcggtg cccgggataa cggcgccagg gggcgggtta agcacgaaat tgtccagtgc
     1141 ccgctttacc acaggagcgg ccgcgatgtc gggcccgccg gatacgggat tgacctgatt
     1201 gagccccttg cggatgtact cgatatcgcc tggcccgatc ggcgctccaa cgccaaggtg
     1261 ctccgtggtc ggcggcgcgc gcatttcgtc gatcgcctgg taggagatcg gacttcgctc
     1321 cggggtgtgg cgctgcgcgc gcagcacatc ctcggtcagc gtggcagagc gcgccagcgc
     1381 attcggctca tagagcgcgg ggctgttttc cagcgcagtg tagcgagcag tcttgtcggt
     1441 gcgcagttcc ggcgtcgttg gcatctcggc ggttgatcgc atcccgccgc gcgggccgaa
     1501 caccgatccc atgccggcgc cgagtacgcc gccaacgccg aacccggtcg cagcgttggt
     1561 caggtagtcg accggtttgc cgctgtaggt cgtgccggcg ccctgcgcgg caccgagcgc
     1621 cgcgccttcg agaccatagc cggctgcccg cgcccctgct tgcccccacc ttcccagccc
     1681 cgtgcccagc cgcgccgcca agccggctcc gcccattccc ggtatgagag cagcaccggc
     1741 gacatcgccc gcgattgaag cgtaaggact acgctcacgc tgctttgcga gtttagcttg
     1801 ttccgcctcc acgcccgcgg aataactggg atagtccccg gacagccatc cggaaagcgc
     1861 gtcccggcga acgtccaatc caaaggtagc ggcgtgaccg gctgctgcgc cagcatcaat
     1921 gagagtttgg ccgatcgtgc cgccgatgct tttcgtcagc tcccagtttg acagcgggcg
     1981 cggcgcctcg tcgtacttaa gctccccaga aggcgtatcg tcataaacga gtggcatcag
     2041 cgcaccttcg tgccagttct tccgttcaat cggaaccgtg acccagctgg cagcttgttc
     2101 gcctcttctt ccgagcgaac atcaacgata ggaccggctg gcacggtgct gggtactgcc
     2161 acggccggca gcggctcgcg cacgtcatag agcgagcgca acgccggatg cgcgctcggt
     2221 tgctgtccgg cgagcatgct atcgagcatc cccttgtggc tccggatgtt ctcctgcgcg
     2281 ttgcgctcgg cgatgttgag aatacgcatg acggtatccg gatcgaggct gatgtcagac
     2341 ccggccattt tctgcacgaa ctcgagctct tgcttggaaa catccttgcc cgcgtaagtc
     2401 ttgatcgtgg cctgcataat agggccgaga ttggacttgt acgattgcgt cgcagccaca
     2461 atgcgctcgg cgtccttgtt ccctgctgct gcagcggcgc ggtaagcctg caatttagcc
     2521 tcggctccaa acccgaacag agccccggaa tttaccgcga gtttggcatc gttgatggct
     2581 tcctgggcac cgaccgcggc aagcgccttt tccttgctgg tatctaggtg cgtgcgcacg
     2641 tagtcgggca ggttgccgta cgctgcgatc gccaggttgg cgcggttctt ctcgtccacg
     2701 cctgttgtcg ttcttgttct gtggctttct gctgctgata cgtggtttcc ttggtccgcc
     2761 agtcctgtac ctgcgcgtcg tagatcgtct tgtttctatc gtcgatatac ttgcgcttgg
     2821 cctccgcgtc gtcgacgatc gccttccaca tcggcgcgct gtacggatcg ttctggtttt
     2881 tcaggtacat atcgcgcgca tatttctcgc gcggactttc cggcgttcgc tcgggcagtg
     2941 cgggcggcgt tggtttttcc gcgacatagg ctttgggcgg gccgccgggc gccgcgcccg
     3001 gactgaggac gggcgcagcg ccggggaccg caccgcgcgc ctcggcaagt cgggtcggcg
     3061 cgggcgtgat gtcggatggc gttaccgggt tttcctctgt cagcggcggg atcgccatgc
     3121 gcccgccgcg cgccatcggc gacggggcat cggtcgcgg
//
LOCUS       DATNBV010000228         4016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2691574,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000228 DATNBV010000000
VERSION     DATNBV010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2691574"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..592
                     /locus_tag="VN627_05530"
     CDS             62..592
                     /locus_tag="VN627_05530"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXD02751.1"
                     /translation="MSTIAAVIGRILIALLFVVSGIMKLMDPGPAATMLSAANLSPAL
                     VFPTALLEIILGLALALGMATRLASLLLAAFTVLTILFFHNQFADPAQLPTILLHVAL
                     VGGLLGVFAHSQIWWSFDALRRRRREDELARDADERARKAELRAERAEGRAEAHDHTV
                     VTPEGTAVRRRRWFWQ"
     gene            630..1166
                     /locus_tag="VN627_05535"
     CDS             630..1166
                     /locus_tag="VN627_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02752.1"
                     /translation="MSGFLFALIACLLASFGARDQALFARLTAAQGQRPLLLATALLA
                     ACATAAFAGWAGARLLTDLAPPLRGTAASLLLVLAGGEMLLLGPGKSPAEPTRSLFAA
                     LVVLAAQQVTDSARLLVLALAVGTAAPIPAALGGALASSAALLTAWLVPTLPERLLRP
                     RRVAGGALALLGGALLIA"
     gene            1264..1716
                     /locus_tag="VN627_05540"
     CDS             1264..1716
                     /locus_tag="VN627_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HXD02753.1"
                     /translation="MGDNAKTAHVANLNGLLADAFALYVKTKNFHWHVKGRNFRDLHL
                     LFDEQAAEIFALTDLIGERVRKNGAATHTSLTSLAADTRIKPQDATDLDAEAMLRELV
                     ADNETFLAQLRATKDTAGEVGDNATDGIVDDWTDQCEQRIWFLRQTVA"
     gene            1899..2543
                     /locus_tag="VN627_05545"
     CDS             1899..2543
                     /locus_tag="VN627_05545"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HXD02754.1"
                     /translation="MPITLIDNASDAAIADWLEERLLVAVGRSPTGIAMTVPGGSTPF
                     PILAALAARPLPWERVAVWPGDDRIVPEDHLASNVGRIRALLEPAGARISPLAEGAVP
                     PRFALAWLGMGGDGHVASLFPNTDPRADDPRRIVRVTPDPLPPEAPFERLSLTIPALL
                     ASDALLFVIRGAEKRALFEAAASGAGLPAHDLPVGRLLAAASHAAPPVPVTCFC"
     gene            2551..3066
                     /locus_tag="VN627_05550"
     CDS             2551..3066
                     /locus_tag="VN627_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXD02755.1"
                     /translation="MLLDRLPAPLHVGALRLADALRRRWWWLAWRLGGRPVRGCRVVV
                     LDGDHRLLMIRHSYGQPHWTLPGGGMDRGEGALAAASREVAEECACRLADPVLLGLAA
                     DTISGARHEVHLVAGWTLDAPLPDGREIVEAGFFAIDALPARVSERLAADLPGYVTRA
                     KAALPPPPRRP"
     gene            complement(3020..>4016)
                     /gene="dinB"
                     /locus_tag="VN627_05555"
     CDS             complement(3020..>4016)
                     /gene="dinB"
                     /locus_tag="VN627_05555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXD02756.1"
                     /translation="VGFAGGRGVVAAASYEARVFGVRSAMASAKALRLCPDLIFRRPR
                     FEVYREVSQAIRAIFLDYTPHVEPLSLDEAYLDVTEDIRGIGSATRIAELIRARIRAE
                     TGLTASAGVSYNKFIAKLASDQNKPDGLCVIRPGEGAEFVAGLPVKRFHGVGPKGAEK
                     MAALGIHTGADLRVRPLAELRAQFGSLADYLYRAARGIDLRQVKADRPRKSIGGERTF
                     HHDISSGPALRETLDGIVEIVWERIARARAEGRTVTLKLRHSDFTTLTRARSLSRAVA
                     GKAEFAAIGHELLAELLPLPQPVRLMGLTLSSLVGEEPEPPRAGAAAAAGQLSLW"
BASE COUNT          542 a         1562 c         1272 g          640 t
ORIGIN      
        1 caacccggaa ccaaactccg ccccagccgc tccccccaac acaccaatgg ggagagcgcc
       61 catgtccacc atcgccgccg tgatcggccg tatcctgatc gccctcttgt tcgtcgtctc
      121 cgggatcatg aagctgatgg acccgggtcc cgccgccacc atgctgagcg cggccaatct
      181 gagcccggcc ctggtgttcc ccaccgccct gttagagatc atcctcggtc tcgccctcgc
      241 gctgggcatg gcgacgcggc tggcctcgct gctgctcgct gccttcaccg tgctgacgat
      301 cctgttcttc cataaccagt tcgccgatcc ggcccagctg cccacgatcc tgctccatgt
      361 cgccctcgtc ggcggcctgc tcggggtctt cgcgcacagc cagatctggt ggtcgtttga
      421 tgccctgcgc cgccgccgcc gcgaggacga gctggcccgc gatgccgacg agcgcgcccg
      481 caaggcggaa ctcagggcgg aacgcgccga gggccgcgcc gaagcgcacg atcacaccgt
      541 ggtcacgccc gaaggcaccg ccgtccgccg ccgccgctgg ttctggcagt aaggtttcca
      601 cgcgggggag ggcctgctaa cccgggcggg tgagcggctt cctcttcgcc ctgatcgcct
      661 gcctcctcgc cagcttcggc gcgcgcgatc aggcgctctt cgcccggttg accgcggccc
      721 agggccagcg cccgctgctg ctggccacgg cgctcctcgc cgcctgcgcc acggccgcct
      781 tcgccggctg ggccggagcg cgcctgctta ccgatcttgc cccgcccctg cggggcacgg
      841 ccgcatccct gttgctggtc cttgccggcg gggagatgct cctcctcggc cccggcaagt
      901 cgccggccga gccgacacgc tcgctgttcg ccgcgctggt agtcctcgcc gcgcagcagg
      961 taaccgacag cgcccgcctg ctggtcctgg ccctcgccgt gggcaccgcc gcgccgatcc
     1021 ccgccgccct gggcggggca ctcgccagca gtgccgcgct cctcaccgcc tggctcgtac
     1081 ctaccctacc ggagcgcctg ctccgtcctc gccgggtggc gggaggtgcc ctggccctcc
     1141 tcggcggtgc gcttctgatc gcctgatccg atttatatat ccaaaactga tcaggaacgc
     1201 gatcaggtca acaggcgtat agccctgcgt atcggcgatg cccgccgcga aagggacccc
     1261 gccatgggtg acaatgccaa gaccgcccac gtcgcgaacc tcaacggcct cctggccgat
     1321 gccttcgcgc tctacgtcaa gaccaagaac ttccactggc acgtgaaagg ccgcaacttc
     1381 cgtgacctgc acttgctgtt cgacgagcag gcggctgaga tctttgccct caccgatctc
     1441 atcggcgagc gcgtccgcaa gaacggcgcc gccacccaca cctcgctgac ctcgctcgcc
     1501 gccgacaccc ggatcaaacc ccaggacgca accgacctcg atgccgaggc catgctgcgc
     1561 gaactcgtcg ccgataacga aaccttcctc gcccagctgc gcgccaccaa ggacaccgct
     1621 ggcgaagtcg gcgacaacgc caccgacggc atcgtcgatg actggaccga ccagtgcgaa
     1681 cagcgcatct ggtttctccg gcagacggtg gcctgaggat accgtccttc gagacggcgt
     1741 tcagcctgcg gcttcacgcc tcctcaggac gaacggggtt aattgtcccg ttcgtggtga
     1801 ggaggggttg aagccgaacg gctgaagccc cgtctcgaac cacgcatagc aatgcgcccc
     1861 ggttccgcat cgcaggcttt cggtgctagg gtgcaggcat gcctatcacc ctgattgaca
     1921 acgcttccga tgccgccatc gccgattggc tggaagaacg cctgctcgtc gccgtcggcc
     1981 gctcgcccac cggcattgcc atgacggtcc ccggcggctc caccccgttc ccgatcctcg
     2041 ccgcccttgc cgcccgcccc ctgccgtggg agcgggtcgc cgtctggccg ggggatgacc
     2101 ggatcgtgcc cgaagaccat ctcgccagca atgtcgggcg gatccgtgcc ctgctcgaac
     2161 ccgcgggcgc ccgcatttcg ccgctggccg agggcgcggt gcccccgcgc ttcgccctcg
     2221 cctggctcgg catgggaggc gatggccacg tcgcctcgct gttccccaat accgatcccc
     2281 gggccgacga tccgcgccgc atcgtgcgcg tcacccccga tccgcttccc cccgaagcgc
     2341 cgttcgagcg cctgagcctg acgatccccg cgctgctcgc cagcgatgcc ctgctgttcg
     2401 tgatccgcgg cgccgaaaag cgcgccctgt tcgaggcggc ggcatcggga gcgggcctgc
     2461 ccgcgcatga cttgccggtc ggccgcctgc tggccgccgc cagccacgcc gcgccccccg
     2521 tcccggtcac ctgcttctgc tgagcggccg gtgctgctgg atcgccttcc cgcgccgctc
     2581 catgtcggag ccctgcgcct cgccgatgcg ctgcggcggc gctggtggtg gctcgcctgg
     2641 cgtctggggg ggcgcccggt gcgcggctgc cgggtcgtgg tgctcgatgg cgatcaccgc
     2701 ctcctgatga tccgccattc ctacggccag ccgcactgga ccctgcccgg cggcggcatg
     2761 gatcggggcg agggcgcgct tgccgccgcg tctcgcgaag tcgccgagga gtgcgcctgc
     2821 cgtctcgccg atccggtcct gctcggcctc gccgccgata ccatatccgg cgcgcgccac
     2881 gaggttcacc tcgtcgccgg gtggaccctt gacgcgcccc tgcccgatgg gcgggagatt
     2941 gtcgaagccg ggttcttcgc catcgatgcc ctgccggccc gggtgtccga acggctggca
     3001 gccgacctgc ccggctacgt taccagagcg aaagctgccc tgccgccgcc gccgcgccgg
     3061 ccctaggcgg ctccggctcc tcgcccacca gcgacgacag cgtcagcccc atcagccgca
     3121 ccggctgggg caggggcagc agctcggcca ggagttcgtg cccgatcgcg gcaaactccg
     3181 cctttcccgc gaccgcccgg ctgagcgagc gcgcgcgggt cagcgtggtg aaatcgctgt
     3241 gccgcagctt gagcgtcacc gtccgcccct cggcccgcgc cctggcgatc cgctcccaca
     3301 cgatctccac gatcccgtcg agcgtctcgc gcagggctgg gccggaggag atatcgtgat
     3361 ggaaggtccg ctccccgccg atcgacttgc gcggccggtc ggccttgacc tgccgcaggt
     3421 cgatccctcg cgccgcgcgg tagagatagt cggccaggct cccgaactgg gcgcgcagtt
     3481 cggccagcgg acgcaccctg aggtccgccc ccgtgtgaat gcccagcgcc gccatcttct
     3541 cggcgccctt gggtcccacg ccatggaacc gcttgaccgg cagcccggcc acgaattccg
     3601 caccctcgcc cgggcggatc acgcacaggc cgtcgggctt gttctggtcg ctggccagct
     3661 tggcgatgaa cttgttgtag ctcaccccgg cgctcgcggt cagcccggtt tccgccctga
     3721 tccgcgcccg gatcagctcg gcgatgcgcg tggccgatcc gatcccgcgg atgtcctcgg
     3781 tcacgtcgag ataggcctcg tcaaggctga gcggctcgac gtgcggggta tagtcgagga
     3841 aaatcgcgcg aatggcctgg ctgacctcgc ggtagacctc gaaccgaggg cggcgaaaga
     3901 tcaggtccgg gcaaagccgc agcgccttgg ccgatgccat ggccgaacgc actccgaaca
     3961 cccgggcctc gtagctggcg gcggccacca ccccgcgccc cccggcaaag ccgacc
//
LOCUS       DATNBV010000229         3737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2693487,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000229 DATNBV010000000
VERSION     DATNBV010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2693487"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VN627_05560"
     CDS             <1..841
                     /locus_tag="VN627_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HXD02757.1"
                     /translation="RVNGLASEDEVRRIVFDARYLVMGLGDVYLGAPVATPVDPRHRL
                     VTTKYNPARTWTPPNVVGIGGAYMCIYGMEGPGGYQLFGRTIQVWNSWQQTGSFRDGK
                     PWLLRFFDRIRFFPVSHEELTEWRRDFPLGRREVRIDEGTFRLADYRAMLADNAASIA
                     AFQQTREAAFAAERAEWQARGEFDRVAALTEEAQAAPAAAEISVPAGCEVIEAPFGGV
                     LWSWAVAEGDRVEAGQTIAVIEAMKMESPVLAPCAGRVRAVYVTERQPVTPGAPLLAL
                     EPA"
     gene            841..2583
                     /gene="atzF"
                     /locus_tag="VN627_05565"
     CDS             841..2583
                     /gene="atzF"
                     /locus_tag="VN627_05565"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HXD02758.1"
                     /translation="MQTTHQIAAEVRSGRSSAEQVMRETIGRIEAYDAVQPQAWISRF
                     APEALIGMARAVDARVRAGEDLPLAGVPFAVKDNIDVAGLETTAACPAFAYRPQAHAE
                     VVQRLCSAGAIPVGKTNLDQFATGLNGTRSPYGAPACVFNLGYVSGGSSSGSAVAVGA
                     GLVPFALGTDTAGSGRVPAAFNGLIGHKPTKGRWSSSGLLPACRSLDCITVFTMDAAD
                     ALLVDGVAAGFDAADPYARRLPDRPVTGQRLGIPLPGQRPWFGDVQAEWLYDQALARL
                     GSLGFTLHPVDFAPLLEAATLLYNGPWVAERTAALEAMLRDRPEAMDPVVREIVAAGR
                     EVSAVAAFRGQYRLMEHARAAEMLWDQVDALAVPTAPTIYRIAEMRANPIALNSHFGV
                     FTNFVNLLDMAAVALPAGYRHNGTGFGISLFAPAGTDRALLQLAETWGNPHPRPALDT
                     GDHMDTIRLAVVGAHLEGMPLHWQLTSRNAKLVTATETAPTYRLYAMADSVPPKPALV
                     HDGSGAAIKVEVYELGVAEFGSFVAEVPPPLAIGTVTLADGSSVKGFVAEPRALDGAE
                     DITALGGWRAFVAR"
     gene            2726..3196
                     /locus_tag="VN627_05570"
     CDS             2726..3196
                     /locus_tag="VN627_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXD02759.1"
                     /translation="MSKPIKLALAALLLPAAALAGAAAAASGPSPIYKLRHDNFEAMG
                     RAMKGTFDQFKKSSPSIPAIQANANALAAAAIKVKGHFPKGTGPQAGVKTEALPAIWE
                     RPADFAAAADRLVSATKGLQAAAATGDLAKIQPAAGAVGGSCKNCHDTFRKAKA"
     gene            3200..>3737
                     /locus_tag="VN627_05575"
     CDS             3200..>3737
                     /locus_tag="VN627_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HXD02760.1"
                     /translation="MTTNSTAAATPAGTPVRLWDLPVRLVHWSFVIGIGLLWWSAENH
                     RMDLHFKVGLVMLGLVVFRLLWGLVGSSTARFASFVKGPAAIRAYLAGQKAGTAAHVV
                     GHNPLGALSVIALIGVLVLQIGLGLFAQDTDAVNSGPLNFLVSYDLAVEISEIHEAVF
                     NVILGLAGLHIAAILYYRF"
BASE COUNT          558 a         1362 c         1226 g          591 t
ORIGIN      
        1 gcgggtcaac ggcctggcgt cggaagacga ggtgcgccgc atcgtcttcg acgcccgcta
       61 tctggtgatg gggctgggcg acgtctatct cggcgccccg gtggccactc cggtggatcc
      121 gcgccaccgg ctggtcacca ccaaatacaa tcccgcccgc acctggaccc cgcccaacgt
      181 cgtcggcatc ggcggggcct atatgtgcat ctacgggatg gagggcccgg gcggctacca
      241 gctgttcggc cgcacgatcc aggtgtggaa ctcgtggcaa caaacgggct cgttccgcga
      301 cggcaagccc tggctcctgc gcttcttcga ccgcatccgc ttcttccccg tcagccacga
      361 ggagctgacc gagtggcgcc gcgacttccc cctcggccga cgcgaagtcc gcatcgatga
      421 ggggaccttc cggctcgccg actaccgggc gatgctcgcc gacaacgccg ctagcatagc
      481 cgccttccag cagacccgcg aagccgcctt cgccgccgag cgggccgagt ggcaggcgcg
      541 cggcgagttc gaccgcgtcg ccgccctcac cgaggaagcc caagccgccc ccgccgccgc
      601 cgaaatctca gtgcccgcag gctgcgaggt catcgaagcg ccgttcggcg gcgtcctctg
      661 gtcctgggcg gttgccgaag gcgacagggt ggaggcgggc cagaccattg ccgtgatcga
      721 ggcgatgaag atggaaagcc cggtcctcgc cccctgcgcc ggccgggtcc gggcagtcta
      781 tgtcaccgag cgccagccgg tcacccccgg cgcgcccctg cttgcgctgg agcctgcctg
      841 atgcagacca ctcatcaaat cgccgccgag gtccgctcgg gccgctcgag tgccgaacag
      901 gtcatgcgcg agacgatcgg gcgcatcgaa gcctacgatg ccgtccagcc gcaagcctgg
      961 atcagccggt tcgcgccaga ggcactgatc ggcatggccc gcgcggtcga tgcccgggtc
     1021 cgcgccggcg aggacctgcc gcttgccggg gtgcccttcg ccgtgaagga caacattgat
     1081 gtggcgggcc ttgagaccac cgccgcctgc ccggcgtttg cctatcgccc gcaggcccat
     1141 gcggaggtgg tgcagcggct gtgcagtgcc ggtgcaattc cggtcggcaa gaccaacctc
     1201 gaccagttcg ccaccgggct caatggcaca cgcagcccct acggcgcgcc agcctgcgtg
     1261 ttcaacctcg gctatgtcag tggcggctcc agttccggct cggccgttgc ggtcggggcg
     1321 ggcctggtgc cttttgccct cggcaccgac actgccggat cgggccgggt tcccgccgca
     1381 ttcaatggct tgatcggcca caagccgacc aagggccgct ggtcgagcag cggtctgctc
     1441 cccgcctgcc gctcgctcga ttgcatcacc gtgttcacca tggacgccgc cgatgccctg
     1501 ctggtggatg gcgtcgcggc gggtttcgat gcggccgatc cctacgcgcg ccgccttccc
     1561 gaccggccgg tcaccggcca gcggctcggc attccgctgc caggccagcg gccctggttt
     1621 ggtgatgtcc aggctgaatg gctttacgat caagcacttg cccggctggg aagcctcggc
     1681 ttcaccctcc acccggtcga cttcgccccg ctgctggaag cggcaaccct gctctacaac
     1741 ggcccctggg tggccgagcg caccgccgcg ctcgaagcca tgctgcggga ccggcccgaa
     1801 gcaatggacc cggtggtgcg cgagatcgtg gcggcggggc gcgaggtcag cgcggtcgcg
     1861 gccttccgcg gccagtaccg cctgatggaa catgcgcgcg cggcggaaat gctgtgggat
     1921 caggtggacg ccctggccgt tccgaccgcg ccgacgatct accgcatcgc cgagatgcgg
     1981 gcgaacccga tcgcgctcaa cagccacttc ggggtgttca cgaacttcgt gaacctgctc
     2041 gacatggccg ccgtggccct ccccgccggc taccgccaca acggcaccgg cttcggcatc
     2101 agcctcttcg cccccgccgg gaccgaccgc gccctgctgc aactcgccga aacctggggc
     2161 aatccccatc cccgccccgc tctcgatacc ggagaccaca tggacacgat ccgcctcgcc
     2221 gtcgtcggcg cgcacctcga aggcatgccg ctgcactggc agcttaccag ccgcaacgcg
     2281 aagctcgtca ccgcgaccga gaccgcgccg acttaccggc tctatgccat ggccgacagc
     2341 gtgcccccca agcccgcgct ggtccatgac ggcagcggcg ccgcgatcaa ggtggaggtc
     2401 tacgaactgg gcgtagcgga gttcggctcg ttcgtcgccg aagtcccccc gccgctcgcg
     2461 atcggcacgg tgactctcgc cgatggctcc agcgtcaagg gcttcgtcgc cgaaccgcgc
     2521 gcgctggacg gcgccgagga catcaccgcg ctgggcggtt ggcgggcgtt cgtcgcgcgg
     2581 tgagttgggc gcgataggct gcgagttcgg gcaacaggac cccggcccgg cgacagatta
     2641 cgacacggcc gagcgttgac cggcgcggcc tgatcggcaa gacaggcgcc gggtctctcc
     2701 tctcgcgcca tggagccgtc gtatcgtgtc gaagccgatc aaacttgccc tcgccgccct
     2761 gctgctcccc gccgccgccc tggccggcgc cgccgcggcc gcttcggggc cgagccccat
     2821 ctacaagctg cgccacgaca atttcgaagc catgggccgg gcgatgaagg gcaccttcga
     2881 ccaatttaag aagtccagcc cgagcattcc ggccatccag gccaatgcca atgcgctcgc
     2941 cgccgccgcg atcaaggtga agggtcactt ccccaagggc accgggccgc aggccggcgt
     3001 gaagaccgag gcgcttccgg cgatctggga gcgtccagcc gactttgccg ccgccgccga
     3061 ccggctggtg agtgccacca agggccttca ggctgccgcc gccaccggag acctcgccaa
     3121 gatccagccg gcggccggcg cggtgggcgg ttcgtgcaag aactgccacg acacgttccg
     3181 caaagccaag gcctgaagca tgaccaccaa ctccaccgcc gccgccaccc ctgcgggaac
     3241 cccggttcgc ctgtgggacc tgccggtccg actggtccac tggagcttcg tcatcggcat
     3301 cgggctgttg tggtggagcg cggagaacca ccggatggac ctgcatttca aggtcgggct
     3361 ggtcatgctc ggccttgtgg tgtttcgcct gctgtggggc ctcgtcggct cgtcgacggc
     3421 gagatttgcg agcttcgtga aggggcccgc cgcgatccgt gcctatctgg ccgggcagaa
     3481 ggcgggcact gccgcccatg tcgtgggcca caatcccctt ggcgcgctca gcgtgatcgc
     3541 gctgattggc gtgctggtgc tgcagatcgg cctcggcctg ttcgcgcagg ataccgatgc
     3601 ggtgaactcc ggcccgctca acttcctcgt ctcctatgat ctggccgtgg aaatcagcga
     3661 gatacacgag gccgtgttca acgtgatcct cggcctggcc gggctgcaca ttgcggcgat
     3721 cctctactac cgcttca
//
LOCUS       DATNBV010000230         5766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1720111,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000230 DATNBV010000000
VERSION     DATNBV010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720111"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1033)
                     /locus_tag="VN627_05580"
     CDS             complement(125..1033)
                     /locus_tag="VN627_05580"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HXD02761.1"
                     /translation="MNSYPEPALALVTQMTAAAAAAPGRAVAFQGAPGCNSHRAALEY
                     APECLPLPCFAFEDALDAVKDGRAGCAMIPIENSQHGRVADIHFLLPESGLSIVGEHF
                     LSIHHDLMALPHADGSLGPFKVAYSHPQALGQSRRFLRERGIVPMSYADTAGAAAYVR
                     ELGKHDAAAIAPRLAAELYGLGIAAERVEDAHDNTTRFVVLAREPLDPAELKGAPAIT
                     TMVFEVNNVPAALYKALGAFATNGVNMTKLESYQKGASFAATMFYADIVGAPGDAGVD
                     RALEELAFHTKELRLLGSYPQARVRG"
     gene            1192..1758
                     /locus_tag="VN627_05585"
                     /pseudo
     CDS             1192..1758
                     /locus_tag="VN627_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831791.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
     gene            1789..2160
                     /locus_tag="VN627_05590"
     CDS             1789..2160
                     /locus_tag="VN627_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXD02762.1"
                     /translation="MASADVKKGEQSFKKCQSCHTVNSGGANGIGPNLFGIAGDKVAE
                     GRGGFAFSDDLKKVGGTWDAAKLDTWLKGPKAMAAGTKMTFAGLTDQQERANVIAYLN
                     SQGGKLTLPAAGAAAAAPAKK"
     gene            2312..3100
                     /locus_tag="VN627_05595"
     CDS             2312..3100
                     /locus_tag="VN627_05595"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXD02763.1"
                     /translation="MTAPAMFRPTAKERAISALLSAAILLGMGVMLVLGLSPDLRQAL
                     RQGDLIAVAFSEPTPTPEPTRKPPPPPAKSSAPKGDAGAAAIRNRATPVVAPPVPVVL
                     VPPPVVAAPVANTGAAASNGAADVAGQGQGAGGIGNGLGGGGLGGNGLGRAPGPDTEP
                     RQIKGKLSQRDLPDGLLAPGEEASVGVRYTVLTDGTVGECVVTKGSGKPALDPLPCRL
                     IRERFRFRPARDGWGRPVNAQIVETHTWVEEPTSEERARIRAGG"
     gene            complement(3167..3832)
                     /locus_tag="VN627_05600"
     CDS             complement(3167..3832)
                     /locus_tag="VN627_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HXD02764.1"
                     /translation="MSRSGRDPGERLKTGRGRTASSARWLQRQLNDPYVKQAKAAGYR
                     SRAAYKLAELDERFGLLKGVKRVVDLGIAPGGWSQVVREKAPAAKIVGIDLLPTDPIE
                     GVTIFQMDFMADEAPAALEGALDGPPDLVVSDMAANTVGHKQTDHLRTMGLVETAVDF
                     AIHTLAPGGAFVAKVFAGGTDQGLLTILKQNFATVKHAKPPASRKDSSEWYVIAQGFK
                     GRR"
     gene            complement(3829..5091)
                     /locus_tag="VN627_05605"
     CDS             complement(3829..5091)
                     /locus_tag="VN627_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HXD02765.1"
                     /translation="MAEGLPPARAKEKAQSGAAGPTDRTAHQVPPGPAASPSARSPGG
                     PSPQPAPVRGSGAFGGSDAALAGRGRPGAPHGGSQGGQAYAAIDLGTNNCRLLIARPS
                     GDNFIVVDAFSRVVRLGEGLAQTGRLSEAAMDRALAALHVCADKLRRRSVHLARSVAT
                     EACRRAENGADFIERARQETGIALDIISAQEEARLAVLGCHILLEQGSGPAMIFDIGG
                     GSTELVLIETGPTIPRILDWQSVPWGVVSLSETCGAEGRTAEERAARYQAMRTIVDES
                     FASFAQRILASHGAFLDQPLRLLGTSGTVTTLASLHLDLPHYDRNMVDGLIVPTAAMR
                     GISARLSGLSPEERRQVPCIGRERADLVVAGCAILESILDIWPADRLGVADRGIREGI
                     LRSLMAAGVHSGGSYRRPRPGSSAESRA"
     gene            5316..>5766
                     /locus_tag="VN627_05610"
     CDS             5316..>5766
                     /locus_tag="VN627_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXD02766.1"
                     /translation="MPLPEMLQKSQIAAVLSNPRLPDNPIVECNAAFEELTGYPASEI
                     LGRNCRFLAGPGTEPWLTETLRSGIRRRQPVMVEITNYRKDGTPFRNAVMVAPIFDDS
                     GELMYFLGSQVEVPGEQARANDERRRVAHDKVAALSARQRAVLVQMAA"
BASE COUNT          896 a         1915 c         2098 g          857 t
ORIGIN      
        1 tcgcgcgccc agcccgaatc gacgccgctg cccgcgctga ccgcgccctg ttcgatagct
       61 gccgcctcca cgcgccgttg ctggcatgcc ccgcagcggg cggcaagcag ttaaccgtat
      121 tgtttcagcc gcgaacgcgg gcctgggggt aggaacccag caggcgcagt tccttggtat
      181 ggaaggccag ctcctcgagc gcgcggtcga ccccggcatc gcccggcgcg ccgacgatat
      241 cggcgtagaa catggtggcc gcgaagctcg cgcccttctg gtagctctcc agcttggtca
      301 tgttgacgcc gttggtggcg aaggcgccga gcgccttgta gagggcggcg gggacgttgt
      361 tcacctcgaa caccatggtg gtgatcgcag gggcaccctt gagttcggcg gggtctagcg
      421 gctcgcgggc gaggaccacg aagcgggtgg tattgtcgtg cgcgtcttcc acccgttcgg
      481 cggcgatccc gagtccgtag agctcggcgg cgagacgcgg ggcgatggcg gcggcgtcat
      541 gcttgcccag ctcgcgcacg taagcggcgg cgccggccgt atcggcatag ctcatcggca
      601 cgatcccgcg ctcacgcagg aagcggcgcg actggccgag cgcctggggg tgcgaatagg
      661 cgaccttgaa cgggccgagc gagccatcgg catgggggag ggccatgaga tcgtggtgga
      721 tcgagaggaa atgctcgccg acgatcgaga ggccgctttc cggcagaagg aagtggatat
      781 ccgcgacgcg cccgtgctgc gaattctcga tcgggatcat cgcgcagccg gcccggccgt
      841 ccttcaccgc atcgagcgcg tcctcgaagg cgaagcaggg cagaggcagg cattcggggg
      901 cgtattcgag cgcggcccgg tgggaattgc agcccggcgc gccctgaaac gcgaccgcgc
      961 gccccggcgc ggcggcggcg gcagcggtca tctgcgtgac gagcgcgagg gcgggctctg
     1021 gataggagtt catgggaatg cgcgcttagg ccctccgggg gcgccgcgca attgcttggg
     1081 cgaccggcgc aaacgcgtgc aggaaccgcg atgcgcgatg cccccttgcg cggcggcgtt
     1141 cccgtcacta gagcgccgtc cggacgccaa gagctattct tcggggcatt catgcaagat
     1201 cgtaacaaca ccattgccgg ttgggcgctg ttttcgggca ttgtcgcgct cggcctaggc
     1261 tcgctgtccg cgcactattt cctcgccgac aagcaggaac gccccgaaaa gatgggctat
     1321 gaaatcgagg gcgtggtttc gtccgaaggc ggcggtgccg ctgccgagga aggcatcgag
     1381 gcccgcctcg ccaaggccga tccggccaag ggcgaagccg tgttcaagaa gtgccagtcg
     1441 tgccacacgg ccaaccaggg cggcgccaac ggcatcggcc cgaacctgtg ggcgaccgtg
     1501 ggtgaggctg tggcccaggg caagggcggc ttcgccttct ccgacgacct caagaagcac
     1561 ggcggcaagt gggattgggc cacgctcgat acctggctga agaatcccaa ggccatggcc
     1621 gcgggcacca agatgacctt cgccggcctg tcggacccga tggaccgcgc caacgtcatc
     1681 gcctacctca acaagcaggg ctccaacctg ccgctgccgg ccgttccggc ggctccggcg
     1741 ggcggtgcgg ccgctgccgg cgcggctccg gccgggatcg acgcggcgct ggcctcggcc
     1801 gatgtcaaga agggcgagca gtcgttcaag aagtgccagt cgtgccacac ggtcaattcc
     1861 ggcggcgcga acggcatcgg tccgaacctg ttcggcattg ccggcgacaa ggttgccgag
     1921 ggccgtggcg gcttcgcctt ctccgacgat ctgaagaagg tcggcggcac ctgggatgcg
     1981 gccaagctcg acacctggct caagggcccc aaggcgatgg ccgcgggcac caagatgacc
     2041 ttcgccggac ttaccgacca gcaggaacgc gccaacgtca tcgcttacct caacagccag
     2101 ggtggcaagc tgaccctgcc tgcggcaggc gcagcggctg cggctccggc caagaagtaa
     2161 tccggcccaa atcagccgat ctggccgaaa agatcgaagg ggcgacgccg cgaggcgccg
     2221 cccttttcgt ttgccggcag tcgccctctg aaactggcag catgggcggc acgttgatga
     2281 ctcgccctgt tcaccatgcg atgccactgc cgtgaccgca cccgccatgt tccggcccac
     2341 cgccaaagag cgcgcgatct ccgcgctgct atcggccgcg atcctgctgg gcatgggggt
     2401 gatgctggtg ctcggcctga gcccggacct gcgtcaggcg ctgcgccagg gcgacctgat
     2461 cgcggtggcc ttctccgaac ccacgcccac ccccgaaccc acccgcaagc cgccaccgcc
     2521 tccggcgaag tccagcgcgc ccaagggtga tgccggggcg gcggcgatcc gcaaccgggc
     2581 aaccccggtg gtcgcgccgc cggtaccggt ggtgcttgtt cccccgccgg tggtcgccgc
     2641 gcccgtcgcg aatacgggcg cggcggccag caacggtgcg gccgacgtgg cggggcaggg
     2701 ccagggggcg ggcggcatcg gcaatggcct gggcggcggc gggctcggcg gcaacggtct
     2761 cggccgcgcg cctggcccgg ataccgagcc gcgccagatc aagggcaagc tgtcgcagcg
     2821 cgacctgccc gacgggctgc tggcccccgg ggaggaagcc tcggtcgggg tgcgctacac
     2881 cgtgctgacc gacggcacgg tgggtgagtg cgtggttacc aaaggcagcg gcaagcctgc
     2941 gctcgatccg ttgccgtgcc gcctgatccg tgagcgcttc cgcttccggc cggcgcggga
     3001 cggctggggc cgcccggtca atgcccagat cgtcgagact cacacctggg tggaagagcc
     3061 gaccagcgag gagcgcgcgc ggatccgggc gggcggatag cgggtttacc tgaactgccg
     3121 cggacaagcc gcgtattgct gttggcgtgc ccgaacaggc cttcggtcaa cggcgcccct
     3181 tgaacccttg cgcgatcacg taccactcgg acgaatcctt gcggctcgcc ggcggcttgg
     3241 cgtgcttcac cgtggcaaag ttctgcttca ggatcgtcag caggccctga tcggtgccgc
     3301 cggcgaagac cttggcaacg aatgccccgc ccggtgcgag ggtgtggatc gcgaaatcca
     3361 ccgcggtctc caccagaccc atggtccgca gatggtcggt ctgcttgtgg cccacggtgt
     3421 tagccgccat gtcggagacg acaagatccg gcggaccgtc cagcgcgccc tcgagcgcgg
     3481 cgggggcctc gtcggccatg aagtccatct ggaagatcgt cacgccctcg atcggatcgg
     3541 tcggcaggag gtcgatgccg acgattttgg cggcgggcgc cttctcgcgc acgacctggc
     3601 tccatccgcc cggggctatc cctaggtcga ccacgcgctt gacccccttg agcaggccga
     3661 agcgctcgtc cagctcggcc agcttgtagg cggcgcggct gcggtagccg gcggccttgg
     3721 cctgcttgac atagggatcg ttgagctggc gctggagcca acgggcagag ctggccgtgc
     3781 gcccgcgccc ggtcttcagg cgctcgcccg gatcgcggcc ggagcggctc atgcgcggct
     3841 ctccgccgaa gagccaggtc gcgggcggcg atagctgccg ccgctgtgga ctccggcggc
     3901 catcaggctg cgcaggatgc cctcgcggat gccgcgatcg gccacgccga gccggtcggc
     3961 cggccagatg tcgaggattg attcgaggat ggcgcagccg gcgacgacga gatcggcccg
     4021 ttcgcggccg atgcagggga cctgccgtcg ctcttccggc gaaaggccgg agagccgggc
     4081 ggagatgccg cgcatggccg cggtcggcac gatcaggccg tcgaccatgt tgcggtcgta
     4141 gtgcggcagg tcgagatgga ggctcgcaag ggtggtgacc gtgccgctgg tgccgagcag
     4201 gcgcagcggc tgatcgagga aggcgccgtg gctggcgagg atgcgctggg cgaaagaggc
     4261 gaagctctcg tcgacgatgg tgcgcatggc ctggtagcgg gcggcacgct cttcggcggt
     4321 gcggccttcg gcgccgcagg tttcgctgag cgagacgacg ccccagggga ccgactgcca
     4381 gtcgaggatg cgcggaatgg tcggcccggt ctcgatcagg accagttcgg tcgacccgcc
     4441 gccgatatcg aagatcatcg ccgggcccga gccttgttcg agcaggatgt ggcaaccgag
     4501 gacggcgagg cgggcctctt cctgggcgct gatgatgtcg agcgcgatgc cggtttcctg
     4561 gcgcgcgcgt tcaatgaagt cagcgccgtt ttccgcgcgg cggcaggcct cggtggcgac
     4621 cgagcgggca aggtggacgc tgcggcggcg cagcttgtcg gcacagacgt ggagcgcggc
     4681 gagcgcccgg tccatcgccg cctcgctcag ccgaccggtc tgggcgaggc cctcgccaag
     4741 gcgcaccacc cggctgaagg catcgacgac gatgaagttg tcacccgagg gccgggcgat
     4801 gagcaggcgg cagttgttgg tgccgaggtc gatcgcggca taggcctgac cgccctgcga
     4861 tccaccatgc ggcgcgcccg gcctgccgcg cccagccagg gcggcatcgc tgccgccgaa
     4921 tgcgcccgaa ccgcgcaccg gcgcgggttg gggcgagggg ccgccgggcg atcgggccga
     4981 aggggaggcg gcaggtccgg ggggcacctg atgggccgtc cgatccgttg gtcctgcagc
     5041 gccgctttgc gctttctcct tggcccgcgc cggcggaagg ccttccgcca tgacttgcac
     5101 tctgtatctg tctgtccgcc gggacggcgg caatgttgcg gagaacctag cggttcggcg
     5161 ccgctccggc aagggcgggc ccggagacgc cgggttgacc gcacggccga tgccacgggt
     5221 ggacaaatgg cgtcccgcgg gcgaaggctc gtcacgcagg aacgtgcccg caaggacacg
     5281 ttgtcgagag ggttgaggag tggcccacag cgatgatgcc attgcccgaa atgctccaga
     5341 agagccagat cgccgcggtt ctcagcaacc cccggctgcc cgacaacccg atcgtggagt
     5401 gcaatgccgc gttcgaggaa ctgaccggct atccggcgag cgagatcctg ggccgcaact
     5461 gccggtttct cgccggcccc ggaaccgagc cctggctcac cgaaaccctg cgcagcggga
     5521 tccgccggcg ccagccggtg atggtggaga tcaccaatta ccgcaaggac ggaaccccgt
     5581 tccgcaatgc ggtgatggtc gcgccgattt tcgacgattc cggcgagctg atgtatttcc
     5641 taggctcgca ggtggaagtt cccggagagc aggcccgcgc caacgacgag cgccggcgag
     5701 tggcgcatga caaggtcgcc gccctgtccg cccggcagcg ggctgtgctg gtgcagatgg
     5761 ccgcgg
//
LOCUS       DATNBV010000231         2692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3638274,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000231 DATNBV010000000
VERSION     DATNBV010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3638274"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1413
                     /locus_tag="VN627_05615"
     CDS             151..1413
                     /locus_tag="VN627_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HXD02767.1"
                     /translation="MASPAASPIKLPFPLSPRELVVMLAFSQALQALAIDAILPALGE
                     VSRELAVTDPNRQQLVVGLFLIGIALGSLIPGTLADRFGRRPVLLGSVISCSVIGGLC
                     ALSRDFTMLLVLRFLGGLLSGGLSAVGSAILRDRFEGDRMASTQSLIFVIFMVVPMVA
                     PTIGQGILLFAGWRWIFGFTAVLGLAVGTWIALRLPESLPADRRLPISAGRIFGSTRE
                     VLLSRESIGYVLSAALTQGIMWGYIQSCSQLLGEHFGAGRSFPLLFGGMAVFMAASNF
                     TNSRIVTRFGARRTGHTALIVYLLAALGQFWLAHRGNETLWQFVPLWTISMICGGFTG
                     ANFSSIAMQPFSRIAGAASSLQLFIRMIIASVIGAMIGQSYDNSARPLSTAMVLASIA
                     SLLLILYSERGRLFRRINPPGTPPQPVP"
     gene            complement(1376..2398)
                     /locus_tag="VN627_05620"
     CDS             complement(1376..2398)
                     /locus_tag="VN627_05620"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HXD02768.1"
                     /translation="MDGSPPASARGVYEFDRAALVAHAQAVMARGSKSFAVASRLFDP
                     VTRERVWLLYAWCRECDDIADAQELGGELGDQAGAELRLARIRALTALALAGEPSGHA
                     SFDCLAVVARECGLTARMAEDVIAGFALDAAEWRPADEADLLLYCYHVAGAVGVMMAA
                     VMGVAADDADTLDRACDLGIAFQLGNIARDLAEDDRAGRCYLPAQWLAEAGIAPGTRL
                     FPGHEAALAGLARRLAALAADYQASARVGAARLPFRCRWAVLAAAGIYGAIARRVAAR
                     GPRAWDTRVRTGTAAKLGHVLGGLVACLHGPAPHPVPRWSRRALAERTGAGPFRAPAG
                     AGSPAG"
BASE COUNT          377 a         1022 c          852 g          441 t
ORIGIN      
        1 cgtccatgtc gacatggatg atcttgcgca ggccgctggc ctcgccgctg tcatccacgt
       61 tgtcgccggc cgccatcgcc gcaccatatc ctattggccg aacgcgaaaa gggggtggtc
      121 acgccgcggt gcagcggcca aagagtcgcc atggcctccc ctgccgcttc cccgatcaag
      181 cttccgttcc cgctatcccc ccgcgaactg gttgtgatgc ttgccttttc gcaggcactg
      241 caggcgctcg ccatcgatgc gatcctgccc gcgctgggcg aggtctcgcg cgaactggcg
      301 gttaccgatc ccaaccggca gcagctggtg gtcggcctgt tcctcatcgg catcgcgctg
      361 ggctcgctga tcccgggaac cctggccgat cgcttcgggc gcaggccggt gctgctgggc
      421 agcgtgatct cctgctcggt gatcggcggg ctgtgcgcgc tgtcgcgcga tttcaccatg
      481 ctgctcgtcc tgcgcttcct cggcggcctg ctctccggcg gcctcagcgc cgtgggcagc
      541 gccatcctgc gcgatcggtt cgagggggac cggatggcca gtacccagtc gctgatcttc
      601 gtcatcttca tggtcgtgcc gatggtcgcg cccaccatcg ggcagggcat cctgctgttc
      661 gccgggtggc gctggatctt cggcttcacc gccgttctgg gcctggccgt gggcacctgg
      721 atcgccctgc gcctgcccga atcgttgccg gccgatcggc gcttgccgat ttcggcgggg
      781 cggatcttcg gctccacgcg cgaggtcctg ctcagccggg aatcgatcgg ctatgtcctg
      841 tccgcggcgc tcacccaagg catcatgtgg ggctatatcc agtcctgcag ccagctgctg
      901 ggcgaacatt tcggcgcggg ccgttccttc cccctgctgt tcggcggcat ggccgtgttc
      961 atggccgcgt ccaacttcac caattcgcgc atcgtcacgc gcttcggcgc gcggcgcacc
     1021 gggcataccg cgctgatcgt ctacctgctc gccgcgctgg gccagttctg gctggcgcac
     1081 cggggcaacg aaaccctgtg gcagttcgtg cccttgtgga cgatcagcat gatctgcggt
     1141 ggcttcaccg gcgccaattt ctcgtcgatc gccatgcagc cgttctcgcg catcgccggc
     1201 gcggcctcat cgctgcagct gttcatccgc atgataatcg cctcggtgat cggcgccatg
     1261 atcggccagt cctatgacaa cagcgcgcgg ccgctctcca ccgcgatggt gctggcttcg
     1321 atcgcctcgc tcctgctgat cctctacagt gagcgcgggc ggctgttccg ccggatcaac
     1381 ccgccgggga ccccgcccca gccggtgccc tgaacggccc ggccccggtc cgttcggcca
     1441 gggcccggcg cgaccagcgc ggcaccgggt gcggggcagg gccgtggagg caggcgacca
     1501 gcccccccag cacgtgcccc agcttggccg ccgtgccggt gcgcacccgc gtatcccagg
     1561 cccgcggccc acgcgccgcc acccggcggg cgatcgcgcc atagattccg gcggcggcca
     1621 gcaccgccca gcggcagcga aacggcagcc gcgccgcgcc cacccgcgcc gaagcctgat
     1681 agtccgccgc caaagctgcc aaccggcggg ccagccccgc cagcgccgct tcgtgcccgg
     1741 gaaagagccg cgttcccggc gcgatccctg cctcggccag ccactgcgcc ggcagatagc
     1801 agcgccccgc ccggtcatcc tcggccaggt cgcgggcgat gttgcccagc tggaaggcga
     1861 ttccaagatc gcaggcccgg tccagcgtgt ccgcgtcatc ggcagccacg cccatcaccg
     1921 cggccatcat cacccccacg gcccccgcca cgtgatagca ataaagcagc agatcggcct
     1981 cgtccgccgg gcgccactcc gccgcgtcga gcgcgaagcc ggcgatcaca tcttcggcca
     2041 tccgggcggt cagcccgcat tcgcgcgcga ccacggcaag gcagtcgaaa ctggcatggc
     2101 cgcttggctc tcccgccagc gccagcgccg tcagtgcccg gatccgcgcc agccgtaact
     2161 ccgcgcccgc ctggtcgccc agctccccgc ccagctcctg tgcgtcggca atgtcgtcgc
     2221 attcccggca ccaggcatag agcagccaca cccgctcgcg cgttaccgga tcgaacaggc
     2281 gcgaggccac ggcaaagctc ttggaccccc gcgccatgac ggcctgcgca tgcgccacca
     2341 gcgccgcccg gtcgaattca tatactcccc tcgcgcttgc gggaggggac ccatccatca
     2401 ctcccctccc gcttgcggga ggggttgggg gtgggcaggg cccaacgccg cgctcgccaa
     2461 caaccaccgc taaccccgtc cacctgaagg aggggaaccg tgccgcgccg cgctcacaag
     2521 tccttcgcca gcatccggaa gatcggcatg tgcggcgaat aggccgccat ccgcgtcagc
     2581 agcaggtcca gttcggtctc tgcaatcagg atcgcctcat gcgccgggcg gatgaagccg
     2641 gccgcgatca tctgccggtt gaaggcaagc aggtgatcga agaacccgaa gg
//
LOCUS       DATNBV010000232         2869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1125094,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000232 DATNBV010000000
VERSION     DATNBV010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125094"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2754)
                     /gene="polA"
                     /locus_tag="VN627_05625"
     CDS             complement(<1..2754)
                     /gene="polA"
                     /locus_tag="VN627_05625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HXD02769.1"
                     /translation="MDAKQHLYLVDGSAYIFRAYHRLPPLTNPAGTPVGAVYGYTTML
                     WKLAQDLHEADGPTHLAVILDKAGTSFRNAMYDQYKANRPPPPEDLVPQFPLIRDATR
                     AFSLACIEEDDLEADDLIASYARAASRQGWDVTIVSSDKDLMQLVGKCAEGENGVPGG
                     CIDMLDTMKNQRIDITEVVEKFGVGPELVGDVLALMGDSVDNVPGIRGIGPKTATKLI
                     QEHGSLEGALAAAPAMKPGKLRESLIEQAEMARLSRRLVALKEDCPLPVALEDFRLGV
                     IPKDPLAAFLAEHGFTSLLKRLDGGQAAPGPKTQLNPAKPQNAAAAAAPAEAPRERPV
                     FPAVDLAAYECVQTAERLAHWISRAFAARMVAVDTETSHLDAMRAELVGISLALGPND
                     ACYVPLGHGGTDMFSERPQQVVKAEALALLKPLLESDAVLKVGQNIKYDINILARVGV
                     NVAPIDDTMIVSFDLDAGRSEEGIGGGHGMDELATRHLAHTTLTFKDICGTGKKAIPF
                     SEVPLDRATQYAAEDADVTWRLHKVLKPRLCEEAATRIYERVDRPLIPVVAQMERHGI
                     KVDRERLARLSGEFAEAIRALECEIYGLCGQEFTIGSPKQLGDILFDKLGYKGGRKGK
                     SGQYSTDQAILEGLAGQGAEVATKVLDWRQLAKLKSTYTDALQAAIHPETQRVHTCYS
                     LVGAQTGRLSSTDPNLQNIPIRTEIGRQIREAFVAEPGNVLLAADYSQIELRLAAHMA
                     DVPELKQAFAQGEDIHSRTAQEMFGLVDRDTRARAKTINFAILYGISRWGLAGRLGVS
                     SDEAQAMIDRYFERFPGIRRYIHATLEGVRENGYSQTLFGRKTWFPRINASNPNERAG
                     SERAAINAPIQGTSADLIKRAMARMDKALADAGLGDVKMLLQVHDELVFEVPDGREEE
                     AAAV"
BASE COUNT          412 a          841 c         1098 g          518 t
ORIGIN      
        1 caccgccgcc gcttcctcct cgcgtccgtc gggcacctcg aacaccagct cgtcatggac
       61 ctggaggagc atcttgacgt caccgaggcc ggcgtcggca agcgctttat ccatccgcgc
      121 catcgcgcgt ttgatcaggt cggcgctggt cccctggatc ggggcattga tcgcggcgcg
      181 ttcggacccg gcacgttcgt tgggattgct ggcgttgatc cgggggaacc aggtcttccg
      241 gccgaacagg gtctgcgaat agccgttctc gcgcacgcct tccagggtcg cgtggatata
      301 gcggcggatg ccggggaacc gctcgaaata gcggtcgatc atggcctggg cctcgtcgga
      361 cgagacgccg aggcggccgg ccaggcccca gcgggaaatg ccgtagagga tggcgaagtt
      421 gatggtcttg gcgcgggcgc gggtatcgcg gtcaaccagg ccgaacatct cctgcgcggt
      481 gcgcgagtgg atgtcctcgc cctgcgcgaa ggcctgcttg agttcgggca cgtcggccat
      541 gtgggcggcg aggcgcagtt cgatctgcga atagtccgcc gcaagcagga cgttgcccgg
      601 ctcggccacg aaggcctcgc ggatctggcg gccgatctcg gtgcggatcg ggatgttctg
      661 gaggttggga tcggtggagg agagccgacc ggtctgcgcg cccaccagcg agtagcaggt
      721 gtggacgcgc tgggtttcgg ggtggatcgc cgcctgcagg gcatcggtat aggtggactt
      781 gagcttggcg agctggcgcc agtcgagcac cttggtagcg acttccgcgc cctgtccggc
      841 gaggccttcg aggatcgcct ggtcagtgga atactggccc gacttgccct tgcgcccgcc
      901 cttgtagccg agcttgtcga acaggatatc gcccagctgc ttggggctgc cgatggtgaa
      961 ttcctggccg cacaggccat agatctcgca ttccagcgcg cggatcgcct cggcgaattc
     1021 gccggagagg cgggcgaggc gctcacggtc gaccttgatg ccgtggcgct ccatttgcgc
     1081 gacgaccggg atcagcgggc ggtcgacgcg ctcgtagatc cgcgtggcgg cctcctcgca
     1141 gaggcggggc ttgagcacct tgtggagccg ccaggtgacg tcggcatctt cggcggcata
     1201 ttgggtggcg cgatccagcg ggacttcgct gaacgggatg gccttcttgc cggtgccgca
     1261 gatgtccttg aaggtgaggg tggtgtgggc gaggtggcgg gtggcgagct cgtccatgcc
     1321 gtggccgccg ccgatgcctt cttcgctgcg gccggcgtcg agatcgaagg agacgatcat
     1381 cgtatcgtcg atcggcgcga cgttcacgcc gacacgggcg aggatgttga tgtcgtactt
     1441 gatgttctgg ccaaccttga ggacggcatc gctttcgagc agcggcttga gcagcgccag
     1501 ggcttcggcc ttgacgacct gttgcggccg ttcggagaac atgtcggtgc cgccatggcc
     1561 gaggggcacg tagcaggcat cgttcgggcc gagggcgagg ctgatgccga cgagctcggc
     1621 gcgcatggcg tcgaggtggc tggtctcggt atcgacggcg accatgcggg cggcgaaggc
     1681 gcggctgatc cagtgggcaa ggcgttcggc cgtctgcacg cattcgtagg cggcaaggtc
     1741 caccgccggg aagaccgggc gctcacgcgg ggcttcggcg ggcgcggcag cggcggcggc
     1801 gttctggggc ttggcggggt taagctgggt tttcgggccg ggcgcggcct ggccgccgtc
     1861 gagccgcttg agcagcgagg tgaagccgtg ttcggcgagg aaggcggcga gcgggtcctt
     1921 ggggatgacg ccgagcctga agtcttccag cgccacgggg agggggcaat cctccttgag
     1981 ggcgacgagg cggcgcgaga ggcgggccat ctcggcctgt tcgatcaggc tttcgcgcag
     2041 cttgccgggc ttcatcgccg gggcggcggc aagggcgcct tcgaggctgc cgtgctcctg
     2101 gatcagcttg gtcgccgttt tcgggccgat gccgcggatg ccggggacgt tgtcgaccga
     2161 atcgcccatc agcgcgagga cgtcgccgac gagctcgggc ccgacaccga atttctcgac
     2221 cacctcggtg atgtcgatcc gctggttctt catcgtatcg agcatgtcga tgcagccgcc
     2281 gggcacgccg ttctcgccct cggcgcactt gccgacgagt tgcatcaggt ccttgtcgga
     2341 ggagacgatg gtcacgtccc agccttggcg cgaggcggcg cgggcatagg aggcgatgag
     2401 atcgtcggcc tcgagatcgt cttcctcgat gcaggcgagg gagaaggcgc gggtggcgtc
     2461 gcggatcagg gggaactggg ggacgagatc ttcgggcggg ggcgggcggt tggccttgta
     2521 ctgatcgtac atggcattgc ggaacgaggt tccggccttg tcgaggatga cggcgaggtg
     2581 agtcggcccg tcagcctcgt ggaggtcctg ggcgagcttc cacagcatgg tggtatagcc
     2641 atagaccgca ccgaccgggg tgcccgccgg gttcgtcagc ggggggaggc ggtggtaggc
     2701 gcggaagatg taggccgacc cgtcgaccag gtagagatgc tgcttggcgt ccatgagcgc
     2761 cttcctagca ctccgggggc ggggcgccag tggggcgggg gcgcaattgt ggcgcaatcg
     2821 tcgccatctg cttgccacaa ttggcggtta cacggtgggc aagctggtg
//
LOCUS       DATNBV010000233         4832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_782760,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000233 DATNBV010000000
VERSION     DATNBV010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782760"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VN627_05630"
     CDS             complement(<1..854)
                     /locus_tag="VN627_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXD02770.1"
                     /translation="MPCLIDMIDGGACSLGDCAAALDAVPFDPADAQSLDHAARWLRR
                     LGNNREFLGDVLMESLTLRHRDEPAQGYGPQVIMLTAPGASSFLRAAIWPGREEGMLG
                     ASGKESFVYGLPHDHNFSFLTHGYFGPGYWSEFYEYDYDGVTGWSGEAARLRHMGRHR
                     LEPDRIMLYRAHVDVHLQLPADSLSVSVNVMHAVPAIRWLDQYTFDVDKGTIAQNVDC
                     GPSETFLRLAVALGGGEALDLAETFARSHPSDRLRVTAWDALAAQETDPGARDALWAR
                     AEGCGSRMV"
     gene            complement(842..1822)
                     /locus_tag="VN627_05635"
     CDS             complement(842..1822)
                     /locus_tag="VN627_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02771.1"
                     /translation="MHIAPEIRALIADPALLERAQERHLAALAEWQAGPGGADVLEAF
                     AAFARGADLARCAPLAALFGADGRAAARFAQGLAAALLPALAAEPFGHVPLRHQSTPA
                     SATLLLAQEASATLTLVVIAGPALAKMPAPRSVAFAPAEEWDVVLAGSGEGRLVERLG
                     EELGEGALAQHPLDLAPGVALGRDVAREALLVDRVPGSLVLLRLNRRRSGAMPVREHE
                     LASGKLIHQAASDPRESRMELAITLLGRMRRADAAPELAAIALEGGRGDSLRWQALRE
                     CLGLDTAAGFRALTTLARSVDDPLAVPAGALRAQLLEQYPVLAELEPCPA"
     gene            complement(1848..1997)
                     /locus_tag="VN627_05640"
     CDS             complement(1848..1997)
                     /locus_tag="VN627_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02772.1"
                     /translation="MNLPKIDVSSLPDLSAATGVFGSLVHKGLMFADDSWIIVMTFLY
                     DATHR"
     gene            complement(2098..2895)
                     /locus_tag="VN627_05645"
     CDS             complement(2098..2895)
                     /locus_tag="VN627_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD02773.1"
                     /translation="MTLATLPLFHRIAGQPVIVLGEGEAAEAKRRLVERAGARVVIDL
                     QEGIDIGARLAFVAHADAGAAEADAIRLRCAGLLVNVVDRPALCDFTVPSLLERGPVL
                     VAVGTGGVSAGLAKALRLRLEALLPPGLGALAEGLGRLRGALRERFPDAGARRRALDA
                     ALAAGGALDPLDPGSAARVEDWLDGSAAGEVAGVAEIVLRSGDPDDLTLREARLLGSA
                     DVVAHEPGVPEAVLLRARADAARLVVGAGEELPRRPDGLIVRVKTRS"
     gene            complement(3028..4230)
                     /locus_tag="VN627_05650"
     CDS             complement(3028..4230)
                     /locus_tag="VN627_05650"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXD02774.1"
                     /translation="MKKLSVAEITRLVDEDMERDSYPAGFPALPPIPGARYTDERFYA
                     LELEHVLKRSWLFAGHIEDIPSPGDYLLFSKVGLSVIVIRGPDDSIRAFHNSCRHRGA
                     AVVNKPAGNLKRFVCPYHAWSYDLSGELRGVPNEYDFGCLDKSERGLVAVNVVNFRGL
                     IFLNVDSDPQPFEDFADGIIRTFSEFPLEKLTPKGTFRIEIPCNWKALQNNFCESYHV
                     TAVHPQTVAKWVVPHSYALHLMRGGHFTATTRKKIAVRIPNPDIPSLDHVDPVYDHHV
                     VGVKMFPNGVSASDPSGFTWMTAWPTGPGTSVLEGVMLGYDDGDSKHEAYWQKRMDDN
                     LMIVSEDLEVLQTIQQSFECGAFSDMICNYRERAIYWHHEEIDRRIGLNRVPPGLAVE
                     PVLGAFVD"
     gene            4544..>4832
                     /locus_tag="VN627_05655"
     CDS             4544..>4832
                     /locus_tag="VN627_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HXD02775.1"
                     /translation="MKDIISARMSRRAILALSGAVMAAKVTPAGATPHGITPTRPVAT
                     GAGKVRGTRSGGISTFKGISFGALMGGMARFRSASPAANWTGVRECTSFGPM"
BASE COUNT          854 a         1718 c         1524 g          736 t
ORIGIN      
        1 accatccggc tgccgcaacc ctcggcccgc gcccacaggg catcccgcgc gcccggatcc
       61 gtctcctgcg ccgccagcgc atcccaggcg gtcacccgca ggcggtcgct cggatggctg
      121 cgggcgaaag tctccgccag atccagcgcc tcgcccccgc cgagcgccac ggccagccgc
      181 aggaaggtct ccgaaggccc gcagtccacg ttctgcgcaa tggtcccctt gtccacgtcg
      241 aaggtatact ggtcgagcca gcggatcgct ggcaccgcat gcattacgtt gaccgaaacc
      301 gagagactgt ccgccggcaa ttgcaggtgc acgtcgacat gggcgcgata gagcatgatc
      361 cggtccggct cgagccggtg ccgccccatg tgccgcagcc gcgccgcctc cccgctccag
      421 ccggtcacgc cgtcgtagtc gtattcgtag aactcgctcc agtagcccgg gccgaaatag
      481 ccatgggtca ggaacgagaa attgtggtca tgcggcagcc cgtagacgaa gctctccttg
      541 ccgctcgccc ccagcatgcc ttcctcgcgt cccggccaga tcgccgcgcg caggaacgac
      601 gacgcgcccg gtgcggtcag catgatcacc tgcgggccat agccctgcgc cggctcgtcg
      661 cggtggcgca aggtcagcga ttccatgagc acgtcgccca ggaattcgcg gttgttgccc
      721 agccgccgca gccaccgcgc ggcatggtcc aggctctgtg catccgccgg atcgaacggc
      781 accgcgtcca gcgcggcggc gcagtcgccc agcgaacagg cgccaccgtc aatcatgtcg
      841 atcaggcagg gcatggctca agctccgcaa gcacgggata ttgttcaagc agttgcgcgc
      901 gcagggctcc agcgggcacg gccagcgggt cgtccacgct gcgcgccagc gtcgtcagcg
      961 cgcgaaagcc cgccgccgtg tccagcccca ggcattcgcg cagcgcctgc cagcgcaggc
     1021 tgtccccccg cccgccttcc agcgcgatcg ccgccagctc cggcgccgca tcggcccggc
     1081 gcatccggcc cagcagggtg atcgccagct ccatccggct ctcgcgcggg tcggaagcgg
     1141 cctggtggat cagcttgccg ctcgccagct catgctcgcg caccggcatg gcgcccgacc
     1201 gccgacgatt gagcctcagc aggacgaggc ttcccggcac ccggtcgacc agcagcgcct
     1261 cgcgcgccac gtcgcggccc agcgccacgc cgggcgccag gtccagcgga tgctgcgcca
     1321 gtgccccctc gcccagttcc tcgcccagcc gctcgaccag ccgcccctcg ccgctgcccg
     1381 ccagcaccac gtcccattcc tccgccggcg cgaaggccac cgagcgcggc gccggcattt
     1441 tcgccagcgc cggcccggcg atcaccacca gcgtcagggt cgcgctcgcc tcctgcgcca
     1501 gcagcagggt ggccgaagcc ggggtggatt gatggcgcag cggcacatgg ccaaacggct
     1561 cggccgccag cgccggcagc agcgccgcgg caagcccctg cgcgaaccgg gccgcggccc
     1621 ggccgtcagc gccgaacaat gcggccaaag gcgcgcagcg ggccaggtcg gcgccgcggg
     1681 cgaaggccgc gaaagcctcc agcacatccg ctcctccggg cccggcctgc cattcggcga
     1741 gggcggcaag atgccgctcc tgcgcgcgtt cgagcagggc cggatcggcg atcagggccc
     1801 ggatttcggg agcgatgtgc atgggcggcc tccgcgacct gtcagcctca gcggtgggtg
     1861 gcgtcgtaga ggaaggtcat cacgatgatc cacgaatcat cggcgaacat caggcccttg
     1921 tggacgaggc tgccgaacac gccggtggcg gcggaaaggt cgggcaggct cgagacgtcg
     1981 atcttgggca ggttcatgga aaaatccccc ttggttgcgc acggggcact ttgccccgca
     2041 caggccaagg gtggctgccg cacggcagcg actgaaatta aaagaccgta atcgcggcta
     2101 actacgtgtt tttacgcgga caatcagccc gtccgggcgg cgcggcagtt cctctcccgc
     2161 ccccaccacc agccgcgccg catcggcccg cgcgcgcagc agcaccgcct cgggaacacc
     2221 cggctcatgg gccacgacat cggccgaacc gagcagccgc gcctcccgca aggtcaggtc
     2281 atcgggatcg ccgctgcgca ggacgatctc ggccacgccg gcaacctcgc ccgccgccga
     2341 accgtccagc caatcctcca cccgcgccgc gctgcccgga tcgagcggat cgagcgcgcc
     2401 gccggcagcc agcgccgcat ccagggcccg ccgccgcgcg cctgcatcgg gaaaccgctc
     2461 gcgcaaggcg ccccgcagcc gccccagccc ctcggccagc gcgcccagcc ccggcggcaa
     2521 caaggcctcc agccgcaacc gcagcgcctt ggccaggccg gcggaaaccc cgcccgtgcc
     2581 cacggccacc agcaccggcc cgcgctccag caggctcggc acggtgaaat cgcacagcgc
     2641 gggccggtcg accacattga ccagcaggcc ggcacagcgc aaccggatcg catcggcctc
     2701 ggcggcccca gcatcggcgt gggcgacgaa ggccagccgc gcgccgatgt cgatcccctc
     2761 ctgaaggtcg atcaccaccc gggcgcccgc ccgttccacc aggcgccgct tggcctcggc
     2821 cgcctcgccc tcgcccagca cgatcaccgg ctggccggcg atgcggtgaa acaggggcaa
     2881 ggtggcaagc gtcatgacga tcccggactt gtgctggcgt gcggaaaccc gccttacccc
     2941 caagcccccg ccttgtcgaa gccccacccg aacggaaggc gatgggccgc tgctccttgc
     3001 gctaggcgaa cagaatgaac cgccggatca atcgacgaag gcacccaaca ccggctcgac
     3061 tgcgagcccg ggcgggaccc ggttcaagcc gatcctccgg tcgatctctt catggtgcca
     3121 gtatatcgca cgctcgcgat agttgcagat catgtcggag aatgcgccgc attcgaatga
     3181 ctgctggatc gtctggagaa cctcgaggtc ctcgctcacg atcatgagat tgtcatccat
     3241 gcgcttttgc caataggcct cgtgcttgga atcgccatcg tcataaccga gcatgacccc
     3301 ctcaagcaca ctggtcccgg gaccggtcgg ccaggcggtc atccaggtga aacccgacgg
     3361 atcagaggca gagaccccgt tcgggaacat cttcacgccg acgacatggt gatcgtaaac
     3421 cgggtcgacg tgatccagcg agggaatgtc ggggttgggt atcctgacgg cgatcttctt
     3481 gcgggtggtc gcggtgaagt ggccgccgcg catcaggtgc agcgcgtagg agtgcggcac
     3541 aacccatttg gccactgtct gggggtgtac cgcggtgaca tggtacgact cgcagaagtt
     3601 gttctgcaaa gccttccagt tgcacgggat ctcgatcctg aatgtcccct tcggagtcag
     3661 cttttccagc gggaattcgg aaaatgttcg aatgatgccg tcggcgaaat cctcgaacgg
     3721 ctggggatcg ctgtccacat tcaggaagat aaggcccctg aagttgacga cattgaccgc
     3781 gaccaggccc cgctcactct tgtcgaggca gccgaagtca tactcgttgg gaacgccgcg
     3841 cagttctcct gaaaggtcgt acgaccaggc atgatagggg cagacgaacc gcttcaggtt
     3901 gccggccggt ttgttgacca ccgcagcgcc gcgatgccgg caggaattgt gaaaggcgcg
     3961 aatggaatcg tccgggcccc tgatcacgat taccgagagc ccgaccttgc tgaacaggag
     4021 gtaatccccg ggcgagggga tgtcctcgat atggccggca aacagccatg agcgcttcag
     4081 cacatgctcg agttccagag catagaaccg ttcgtccgtg tagcgcgcac cgggaatggg
     4141 cggaagtgcc gggaacccgg cggggtagct gtcccgttcc atgtcctcgt caacaagccg
     4201 cgttatctcg gccacggaca gttttttcat ctgatcctct tccgtttttg gcatgcctgt
     4261 cggccagcga ttgctatcca gtctatgctc ggccgattag gcaagcaaga agggtagtat
     4321 tctggtgatc gggtacggcg taaaccggca ctgctcggca gcacattgac cagcaagctc
     4381 gccaggcgcg gccggatctc atgccctgag cgtccccttg ccggcacggg ccgccaactg
     4441 tcggccgatc agtccatcat caacccgacc ttaccgcttg gttgggttct cattttcgct
     4501 acattgacaa gaaaaacact tctccgccga tgggagttgc acaatgaaag acataataag
     4561 cgcaaggatg tcacgtcgcg ccatattggc gctgtccggt gcagtgatgg cggcgaaagt
     4621 aaccccggca ggtgccacac ctcacggcat aacgccaacc cgcccggttg cgaccggcgc
     4681 cgggaaggtg cgcggcacgc gttctggagg aatatccacc ttcaagggta tttcgttcgg
     4741 cgcattgatg gggggaatgg caagatttcg gtccgcatcg cctgctgcca attggaccgg
     4801 ggtgcgcgaa tgcactagct ttggccccat gg
//
LOCUS       DATNBV010000234         5059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1721755,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000234 DATNBV010000000
VERSION     DATNBV010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721755"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VN627_05660"
     CDS             complement(<1..577)
                     /locus_tag="VN627_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02776.1"
                     /translation="MAETINPARDDYASDNIKKPANVAVLTGTLLILLLVAAVAVSPR
                     VPIYDEGFYLESAWFLRRHFDFFVLMNARLDLAAGPFYAYLHHFAGPLTGLRPPAIRY
                     VNVGALIIAILAIGSTIRRLGYDQPMARAAMLLAVPMIWPTSGLALTEIPALTLASLA
                     VLPVVVASRASAARQWPLLLLAGLCAGLAITA"
     gene            complement(808..1704)
                     /locus_tag="VN627_05665"
     CDS             complement(808..1704)
                     /locus_tag="VN627_05665"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HXD02777.1"
                     /translation="MAHRPIRKAVFPVAGLGTRFLPATKAIPKEMLPIIDRPLIQYAV
                     DEAREAGIEQLIFVTGRGKTAIVEHFDMAFELETTMAGRAKSLDVLEPTRIEPGNLVT
                     VRQQVPLGLGHAIWCARAIVGDEPFAIFLPDELMIAHQGSTGCMKQMVAAYNEVGGNL
                     ISVLEVPEEEVSSYGVIAPGARPEGFGGALTEVMGLVEKPRREDAPSNLIISGRYILQ
                     PEVMRTLEGQEKGAGGEIQLTDAMARMIGTQPFHAVTFAGRRYDCGSKAGFVEATLAL
                     ALERPDMADDVRAIMQRLLAGG"
     gene            1865..3148
                     /gene="murA"
                     /locus_tag="VN627_05670"
     CDS             1865..3148
                     /gene="murA"
                     /locus_tag="VN627_05670"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXD02778.1"
                     /translation="MDKIIIEGGKRLSGTLPISGAKNSALTLLPCTLLTDEPLTLRNL
                     PRLADIDGFQHLLNQFGVSTTIAGSRPEDFGRVMTLAATRMTSTVAPYELVRKMRASI
                     LVLAPMLARMGEATVSLPGGCAIGNRPIDLHLKALEALGARIELAAGYVRAIAPDGGL
                     PGGRYSFPVVSVGATENALMAAVLCKGKSTLHNAAREPEIVDLCRLLVAMGAEIEGIG
                     TSDLTIHGVPRLHGATYRVMSDRIEAGSYACAAAITGGEVTLKHAVVEEMEATVQALR
                     DAGAIVEPRDDGLYVAADGPLRAVTLSTAPYPGFATDMQAQLMAMLCLAQGSSVLTET
                     IFENRYMHVPELNRMGAHIETKGRTAVVHGVKGLTGAEVMATDLRASMSLVIAGLAAE
                     GQTQVHRLYHLDRGYERLEEKLALIGAQVERVGGD"
     gene            3245..3490
                     /locus_tag="VN627_05675"
     CDS             3245..3490
                     /locus_tag="VN627_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02779.1"
                     /translation="MALLKLAFAGAVAYGLYRYSQKQKTENPGHAAFATGETSGENFA
                     KVRHAGPEGMKSDPVHWDKADEASDESFPASDPPATY"
     gene            complement(3752..3979)
                     /locus_tag="VN627_05680"
     CDS             complement(3752..3979)
                     /locus_tag="VN627_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HXD02780.1"
                     /translation="MSDLFHPPVKRSVEIAGHKTSISLEPVFWAMLKAAAAEEGVPIN
                     ALVARIDAARLEAEVPPGLASAIRVWLVGRG"
     gene            complement(4098..4820)
                     /gene="phbB"
                     /locus_tag="VN627_05685"
     CDS             complement(4098..4820)
                     /gene="phbB"
                     /locus_tag="VN627_05685"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HXD02781.1"
                     /translation="MTRVAIVTGGSRGIGKAICLALRDQGVSVVANYAGNDAAAQQFT
                     ADTGIPAYRWDVGDHEACLAGCAEVAAKHGPIDIVVNNAGITRDGVLHRMTFDDWNDV
                     MRINLGGCFNMAKATFPSMRERGWGRIVNIGSVNGQAGQYGQVNYAAAKSGIHGFTKA
                     LAQEGAKFGVTVNAIAPGYVDTDMVAAVPAPVLEKIVAKIPVGRLGHAEEIARGVAFL
                     TSEEAGFVTGSTMSINGGQHMY"
BASE COUNT          890 a         1764 c         1582 g          823 t
ORIGIN      
        1 gggcggtgat ggcgaggccg gcacacaggc cagccagcag gagcaggggc cattgccgcg
       61 cggcgctagc ccggcttgcg actacgaccg ggaggacggc gagactggcc agagtcaggg
      121 cgggaatttc ggtaagcgcc aacccgctgg tcggccagat catcggtact gcgagcaaca
      181 tggccgcccg tgccattggc tggtcataac ccagacggcg aattgtcgag cctattgcga
      241 gaatggcaat gatcaacgcg ccgacgttga catagcgaat ggccggcggc cgcaaaccgg
      301 tcaacggtcc tgcgaaatgg tgcaggtagg catagaacgg ccccgccgcc agatcgaggc
      361 gggcgttcat caggacgaaa aagtcgaagt ggcggcgaag gaaccatgcc gattcgaggt
      421 agaacccctc atcgtagatc ggtacccggg gcgagacggc caccgctgct accagcagca
      481 gaatcagcaa ggtcccggtc aggacagcca cattcgctgg tttcttgata ttatcagagg
      541 cgtagtcatc gcgcgcggga ttgatggttt cagccacttt gtgcgtatac ccacatgttg
      601 ttgatcatga agctgagtga cggataggca ggctcaccct gatcctcatc gcggcaagac
      661 gtgggccgat tctggcaggc aacaatgctc ggcgcaacag gctgcgcacg gcaatcagtc
      721 cttttggcgt cgaagcgaag gtccgcctgg ctgtaaatga gcatgacccg gccttcgttc
      781 gaccggtcag cgcggtgccc cgtgcaatca gcccccggcc agcagccgct gcatgatcgc
      841 gcgcacgtcg tcggccatgt ccggccgctc cagcgccagg gccagcgtcg cctcgacgaa
      901 gcccgccttg gacccgcagt cataccgccg gccggcgaag gtcaccgcgt ggaagggctg
      961 ggtcccgatc atccgggcca tcgcgtcggt cagctggatt tccccgcccg cgcctttctc
     1021 ctgcccctcc agcgtgcgca tcacctcggg ctggaggata tagcgcccgg aaatgatcag
     1081 gttcgacggc gcatcctcgc gcctcggctt ttcgaccagg cccatgacct cggtcagcgc
     1141 cccgccgaag ccttccggcc tcgcccccgg cgcgatcacg ccgtagctgg agacctcctc
     1201 ctccggcact tccagcaccg agatcaggtt gccgcccact tcgttgtaag ccgcgaccat
     1261 ctgcttcatg cagccggtcg aaccctggtg tgcgatcatc agctcgtccg gcaggaagat
     1321 cgcgaagggc tcgtcgccca cgattgcccg ggcacaccag atcgcgtggc ccaggcccag
     1381 cggcacctgc tggcgcacgg tgacgaggtt gcccggctcg attcgcgtcg gttcgagcac
     1441 gtcgagcgac ttggcgcgcc cggccatggt ggtttccagc tcgaaggcca tgtcgaaatg
     1501 ctcgacgatc gccgtcttgc cgcgcccggt cacgaagatc agttgctcga tccccgcctc
     1561 gcgcgcctcg tccaccgcat actggatcag cggccggtcg atgatcggca gcatttcctt
     1621 ggggatcgcc ttggtggcgg ggagaaaccg ggtccccagc ccggcaacgg ggaaaacagc
     1681 cttgcggatc ggcctatgcg ccatggtcat ccaatatccc gatcaggttt tcgacaagta
     1741 tcgtgaacag tcacggcgtg cccgcgttcc atagaagcag atgcgattcc gcggtgcaat
     1801 cgagattcac aggcctcttg cccggcccat tgcgccccct tccgccccgc cctaaacgtg
     1861 cgcgatggac aagatcatca tcgagggcgg caagcgcctg tccggcaccc tgccgatatc
     1921 cggagcgaag aactcggcgc tcaccctgct gccctgcacc ctgctgacgg acgagccgct
     1981 gaccctgcgc aacctcccgc gcctggccga tatcgacggg ttccagcacc tgctcaacca
     2041 gttcggcgtg tcgaccacga tcgccggctc gcggccggag gatttcggcc gggtgatgac
     2101 cctggccgcc acccggatga cgtcgaccgt cgcgccctac gagctggtcc gcaagatgcg
     2161 cgcctcgatc ctggtgctcg ccccgatgct ggcgcgcatg ggcgaggcga ccgtgtcgct
     2221 ccccggcggc tgcgccatcg gcaaccgccc gatcgacctg cacctcaagg ctctcgaagc
     2281 cctcggcgcc cggatcgaac tcgccgccgg ctacgtccgc gccattgcgc ccgatggggg
     2341 gctccccggc gggcgctatt ccttcccggt cgtctcggtc ggcgcgaccg agaacgcgct
     2401 gatggccgcc gtcctgtgca agggcaagtc caccctccac aacgccgcgc gcgagcccga
     2461 gatcgtcgac ctgtgcaggc tgctcgtcgc catgggcgcc gagatcgagg gcatcggcac
     2521 ctccgacctc accatccacg gcgtcccccg cctccacggc gcgacctacc gggtcatgtc
     2581 cgaccggatc gaggccggct cctacgcctg cgccgccgcc atcaccggcg gcgaggttac
     2641 cctgaagcac gccgtggtcg aggaaatgga agccacggtc caggccctgc gcgatgccgg
     2701 cgcgatcgtc gagccgcgcg acgacgggct ctatgtcgcc gccgatggcc ccttgcgcgc
     2761 cgtcaccctg tcgaccgcgc cctatccggg cttcgccacc gacatgcagg cgcagctcat
     2821 ggccatgctg tgcctcgcgc aaggttcctc ggtgctgacc gagacgatct tcgagaaccg
     2881 ctacatgcac gtgcccgagc tcaaccgcat gggcgcccac atcgaaacca agggccgcac
     2941 cgccgtggtc catggggtca agggccttac cggcgccgaa gtcatggcaa ccgacctgcg
     3001 cgcttcgatg agcctggtca tcgccggtct cgcggcggag gggcagaccc aggtccaccg
     3061 cctctaccat ctcgatcgcg gctacgagcg gctggaagag aagctcgcgc tgatcggcgc
     3121 acaggtggag cgcgtcggcg gggattgagc ccggccgaat gcatatagcc cgcatcggcg
     3181 ggaaccgatg cgggtggccg tgcctgtttg ggtgaaacct tcatccttat gcaggagact
     3241 tccaatggct ttgctcaagc tcgcctttgc cggcgccgtg gcctacggcc tctaccgcta
     3301 ttcccagaag cagaagaccg aaaaccccgg ccacgcggcc ttcgccactg gcgaaacctc
     3361 gggcgaaaac ttcgccaagg tccgccatgc cggccccgaa ggcatgaaga gcgatccggt
     3421 ccactgggac aaggccgacg aagccagcga cgaaagcttc ccggccagcg acccgcccgc
     3481 aacctactga tcgcaaaaac gcaaccctcc cccctcccgc aggcgggagg gcgcccgtca
     3541 tcactcccct cccgcctgcg ggaggggcaa ggggtgggca ggaacaactc ccacctgatc
     3601 tcgtcatgct gaacttgttt cagcatccat ggcgcagcat tgcgctctgc ctgagacctc
     3661 ggactggtcg cgacgccccc atggatgctg aaacaagttc agcatgacgg tgcagggggg
     3721 tgggggaagc cagaggtaaa cccgcggcgc cctacccccg ccctaccagc cacacccgga
     3781 tcgcactcgc cagccccggc ggcacctccg cctccagccg cgcggcatcg atccgcgcca
     3841 ccagcgcatt gatcggcacg ccttcttccg ccgcggccgc cttgagcatg gcccagaaaa
     3901 ccggctcgag gctgatcgaa gtcttgtgcc cggcaatctc gaccgatcgc ttgaccggcg
     3961 ggtgaaacag gtcgctcacg cgccaggatc ctccccgctt ttgcgaagca caagggcaga
     4021 ggcggcaggc gcgtagcgcc tgagggaggg gtatttaccc gcgccgctgt tcgcagcccg
     4081 gcatgggcca aattccctca atacatgtgc tgcccgccgt tgatcgacat cgtcgatccg
     4141 gtcacgaacc ccgcctcttc cgaggtcagg aaggcgacac cccgggcgat ttcctcggca
     4201 tggccaaggc gtcccaccgg gatcttggcg acgatcttct ccagcaccgg cgcgggcacg
     4261 gccgccacca tgtcggtgtc gacatagccc ggggcgatgg cgttgacggt cacgccgaac
     4321 ttggcgcctt cctgggcaag ggccttggtg aagccgtgaa tgccgctctt ggcagcggcg
     4381 tagttgacct ggccatattg cccagcctgc ccgttgaccg agccgatgtt tacgatgcgc
     4441 ccccagcccc gctcgcgcat ggaggggaag gtggccttgg ccatgttgaa gcaaccgccc
     4501 aggttgatcc gcatgacgtc gttccagtcg tcgaaggtca tccggtggag cacgccgtcg
     4561 cgggtgatgc cggcattgtt gacgacaatg tcgatcggcc cgtgcttcgc cgcaacctcg
     4621 gcgcagccgg cgagacaggc ctcgtgatcg cccacgtccc agcggtaggc gggaatgccg
     4681 gtgtcggccg tgaactgctg tgcggccgca tcgttgcccg cgtagttggc gacaacggaa
     4741 acgccctggt cccggagggc caggcagata gccttgccga tgccgcgcga accgccggta
     4801 acaatagcta cgcgtgtcac tttagcccct cctattgtcg caataacgat aatgaatgga
     4861 ggtggaggct aggaaacgcc gccgacaccg gcaagcagga ccttggtcct ccatacgaat
     4921 tcacgcgggg gggcgatgcc gcggggcaat cacccgcggc cgggtcagaa gcggaactgc
     4981 gtacccacat agacggcctg cccgtccttc tgtccgtcaa ccgcctgaag cacgcggtcg
     5041 cgctcctgcg aataccgta
//
LOCUS       DATNBV010000235         4187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1125841,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000235 DATNBV010000000
VERSION     DATNBV010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125841"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..497)
                     /locus_tag="VN627_05690"
     CDS             complement(138..497)
                     /locus_tag="VN627_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXD02782.1"
                     /translation="MGSSTFSILGPDLAIKGDLVASADLHIDGKVEGDIACAALVQGE
                     SGVIQGAIRADSARLAGTVTGSVDAGTLVILRTARIEGDVAYETLTIEQGAQVEGKLS
                     PKRSAEPKLAIAGGTEA"
     gene            complement(499..1635)
                     /locus_tag="VN627_05695"
     CDS             complement(499..1635)
                     /locus_tag="VN627_05695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXD02783.1"
                     /translation="MRSWFPEREFFMRSEGHVRFIRISSKLQLRAAAAASALVLAWVV
                     TMAWAGMASLVASWHHEDLLDREAKVVSAESRVAAYRANVGKVADDIARRQAFIETMV
                     KTHVGDLPADVRAGETVSNSSGEAAKTVDRISAAIPEAAPLARIEARQLAFVESLTRL
                     ADRRSAAAEAAIRKLGLDPGRMLASANGSSAEGGPSLSLSTTADGSIDPRFERLGLSL
                     ARMDALRRGLEGIPQVLPASLQYISSGFGFRPNPFGGRGGEFHPGLDFKGPYGAPIYA
                     AARGRVSFVGQRSGYGNCVEVDHGNGLVTRYAHMSAFRTHVGTPVAAGQLIGQIGNTG
                     RSTGPHLHFEVRINDVAVNPRPFLEAAPHVLEEARDPFRAGRQL"
     gene            1880..3166
                     /locus_tag="VN627_05700"
     CDS             1880..3166
                     /locus_tag="VN627_05700"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HXD02784.1"
                     /translation="MSQAAPVSASKAESPEVLIARLAKAGRAAQRVLASLPSEQKAAA
                     LRAAAAALRAAEPDILAANAADIANGEANGLSAAMLDRLKLDPARLAGIANAVDQVAD
                     LPDPVGQVIDNTHRPNGLDLRRVRVPVGLIGIIYESRPNVTADAAALCLRSGNAVLLR
                     GGSEAVHSNRAIHAALAKGLEQAGVPAEAVQLVPTQDRAAVGAMLAAAGLIDMIVPRG
                     GKSLVARVQADARVPVLAHLDGICHTYLHSAADPAMAKSIALNAKMRRTGICGAMETL
                     LLDAAFPGSVEVVQALLEAGCELRGDARAQALDPRVVPAAANDWDTEYLDAILSVAVV
                     DGLDAALAHIAAHSSAHTDAIVTSDPAAAERFLNEVDSAIVMVNASTQFADGGEFGLG
                     AEIGIATGRLHARGPVALEGLTTYKWQVRGTGQTRP"
     gene            3177..3827
                     /locus_tag="VN627_05705"
     CDS             3177..3827
                     /locus_tag="VN627_05705"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HXD02785.1"
                     /translation="MIRTGPKTGLLGGSFNPAHGAHRRISLAALGALGLDEVWWLVSP
                     GNPLKSETGMAPLAARFASAQAQARRAPIRVSAIERQFGTRYTAQTLRRLLLRYPRRR
                     FVWLMGADNLAQFHRWKDWRGIARMVPIAVVARPGYDGAAVASPAMAWLGRYRTSAAS
                     LTNRAAWSAPVLVILRFDPDPRSATAIRQADPNWASRHSSQAPRDHLTLHPIEPNQ"
     gene            3895..>4187
                     /gene="rsfS"
                     /locus_tag="VN627_05710"
     CDS             3895..>4187
                     /gene="rsfS"
                     /locus_tag="VN627_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXD02786.1"
                     /translation="MSIEPSPATGGQPAAPLPPSIPGSLHALVLQSLDDDQAQDIVSI
                     PLEGKSAIADHMVIASGRSTRQVAAMAQKLAERIKHGGFGNSRIEGLPAADWVL"
BASE COUNT          642 a         1511 c         1382 g          652 t
ORIGIN      
        1 tcgctcctca gggcgaaggg gattttttga aacaggccct tataaatcgc ccggtcgtgc
       61 tgaggagccc tgaagccgaa ggcaaaaggg cgtctcgaag caccgaggcg cgtgcactcc
      121 gacgcgcgcc agtggcctca agcctcggtc ccgccggcaa tcgccagctt gggctcggcg
      181 ctgcgcttgg ggctgagctt gccttccacc tgcgcgccct gctcgatggt cagggtttca
      241 taggccacgt caccctcgat ccgcgccgtg cgcaggatca ccagcgtgcc cgcgtcgacc
      301 gagccggtta cggttccggc cagccgcgcg ctgtcggcgc gaatggcgcc ctggatcacg
      361 ccgctctcgc cctggaccag cgcggcgcag gcgatatcgc cttccacctt gccgtcgatg
      421 tgcagatccg cgctggcgac gaggtcgccc ttgatcgcca ggtcaggacc caggatcgag
      481 aaggtcgagc tgcccatgct agagctgacg gccggcgcgg aaggggtctc gcgcttcttc
      541 gagaacatgg ggggcagcct ccagaaaggg gcgtgggttc acggccacgt cgttgatcct
      601 gacttcgaaa tgcagatgcg gtccggtcga gcggcccgta ttgccaatct gcccgatcag
      661 ctgcccggcg gcaaccggcg ttcccacgtg cgtgcggaag gcgctcatgt gggcatagcg
      721 ggtgacgagg ccattgccgt ggtccacttc cacgcagttg ccatagcccg accgctggcc
      781 gacgaagctg acccgcccgc gcgccgccgc atagatcggc gcgccgtagg ggcccttgaa
      841 gtcgagcccc ggatggaatt ccccgccgcg tccgccaaac gggttggggc ggaagccgaa
      901 gcccgaggaa atgtattgca ggctcgccgg gagcacctga gggatcccct cgagcccgcg
      961 ccgcagcgca tccatgcgcg ccaggctcag gccgagtcgc tcgaagcgcg gatcgatcga
     1021 gccgtcggcc gtggtgctca agctgagcga cggcccgccc tcggcgctgc tgccattcgc
     1081 cgaggccagc atgcgcccgg gatccagacc cagcttgcga atcgccgctt ccgccgcggc
     1141 gctgcgccgg tcagccaggc gggtcaggct ttcgacgaag gccagctggc gcgcctcgat
     1201 ccgggcgagg ggggcggcct cgggaatggc cgcgctgatc ctgtcgaccg tttttgccgc
     1261 ttcgccgctg ctgttggata cggtttcccc ggcgcggacg tcggccggca ggtcgccgac
     1321 gtgggttttc accatggtct cgatgaaggc ctgccgccgc gcgatatcgt cggccacctt
     1381 gccgacattg gcgcggtagg cggcgacccg gctctcggcg gagaccacct tggcctcgcg
     1441 gtcgagcagg tcttcgtgat gccacgaggc caccagcgag gccatcccgg cccaggccat
     1501 ggttaccacc caggcgagca caagcgcaga tgcggcagcc gcggcgcgca gctggagctt
     1561 gctcgatatg cggatgaagc ggacatggcc ttcggagcgc atgaagaact cgcgctccgg
     1621 aaaccagctc cgcacgcggt tcaccaccgc gcccggttgc agccttggca agatgacccc
     1681 tgtttgttgt tctcgacccg tccggcgccg cggctagcag ggccgctgcg acgaatcgaa
     1741 tcgcggttcc cggagtcagg acgagtcgaa cgagtagcgc gacgaagcgt tgttgcgttg
     1801 gtaaccatcc ggcaggccgc gtccgcgaat cgagccgact cgggctgaca actccggcgt
     1861 caggtgatag gcgctcccca tgtcccaagc cgcacctgtc tccgccagca aggcggaaag
     1921 ccccgaggtc ctgatcgccc gcctggccaa ggccggccgc gcagcccagc gggtgctggc
     1981 gagcctgccg agcgagcaga aggctgcagc cctgcgcgcc gccgccgccg ccctgcgcgc
     2041 ggccgagccg gatatacttg ctgccaatgc cgcggatatc gccaatggcg aggccaatgg
     2101 cctgtccgcc gccatgcttg accggctgaa gctcgatccc gcgcgccttg ccgggatcgc
     2161 caatgcggtg gaccaggtgg ccgacctgcc ggatcccgtc gggcaggtga tcgacaacac
     2221 tcatcgcccc aacgggctgg acctgcgccg cgtgcgcgtg ccggtcgggc tgatcggcat
     2281 catctatgaa agccgtccca acgtaactgc cgatgccgcg gcgctgtgcc tgcgctcggg
     2341 caatgccgtg ctcctgcgcg gcggctccga ggcggtccat tccaaccgtg cgattcacgc
     2401 cgcgctcgcc aaagggctgg agcaggccgg ggttcccgcc gaagcggtcc agctcgtccc
     2461 cacgcaggac cgcgcggccg tcggcgccat gctggcggcg gcgggcctga tcgacatgat
     2521 cgtgccgcgc ggcggcaaga gcctcgtcgc ccgggtccag gccgatgccc gcgtgcccgt
     2581 gctcgcgcac ctcgatggca tctgccacac ctatctgcat tccgccgccg atccggccat
     2641 ggcgaagtcg atcgcgctca acgccaagat gcgccgcacc ggcatctgcg gagcgatgga
     2701 aaccctgctg ctcgatgccg ccttccccgg ctcggtcgag gtggtgcagg ccctgctcga
     2761 agcaggctgc gaactgcgcg gcgatgcccg ggcccaggcg ctcgatccgc gcgtggttcc
     2821 ggccgccgcg aacgactggg acactgaata cctcgacgcg attctatccg tcgcagtggt
     2881 ggatggcctc gatgccgccc tggcgcacat cgccgcccac tcatcggccc ataccgatgc
     2941 gatcgtcacc agcgatccgg cggcggccga gcggttcctc aacgaggtcg attcggcgat
     3001 cgtcatggtc aacgcgtcga cccagttcgc cgatggcggg gagtttggcc tcggcgcgga
     3061 gatcggcatt gccaccgggc ggctccacgc ccgcggcccg gtcgcgctgg aaggcctcac
     3121 cacctacaag tggcaggtcc gcggcaccgg ccagactcgg ccctgaggtc aaggccctga
     3181 tccgcaccgg cccgaaaacc ggcctcctcg gcggctcgtt caaccccgcc catggcgcgc
     3241 accggcgaat cagcctcgcg gcgctggggg cactggggct ggacgaggtc tggtggctgg
     3301 tttcccccgg gaatccgctc aagtccgaaa cgggcatggc gccgcttgcc gcccgcttcg
     3361 cctcggccca ggcccaggcc cgccgcgccc cgatccgcgt ctccgcgatc gagcgccagt
     3421 tcggcacccg ctacaccgcg caaaccctgc gccggttgct gctgcgctat ccccgccgcc
     3481 gcttcgtctg gctcatgggc gccgataatc tggcccagtt ccaccgctgg aaagactggc
     3541 gcggaattgc ccgcatggtg ccgattgccg tcgtcgcccg tccgggctat gatggcgccg
     3601 ctgtcgcgag ccccgcgatg gcctggctcg ggcgttaccg aacatccgcc gccagcttga
     3661 ccaatcgggc agcgtggagc gcaccagtac tggtgatcct gagattcgat cccgatccgc
     3721 gatcggccac ggcaatccgc caggctgatc caaactgggc cagccggcat tccagccagg
     3781 ccccgcgcga tcatctcacc ctccatccca tagagccgaa ccaatgaaac cgcgcttttc
     3841 ctcgcgcgtt tcggctgctg tcccaatgcg tgcgaaggag cacaccccgg ccgcatgagt
     3901 atcgaaccca gtcccgccac cggtgggcaa cctgccgccc cgctgccccc gtcgatcccc
     3961 ggatcgctcc acgccctggt gctccagtcg ctggatgacg accaggcaca ggacatcgtc
     4021 tcgatcccgc tggaaggcaa atcggccatt gccgatcaca tggtgatcgc ctccggccgg
     4081 tcgacccggc aggtcgcggc catggcccag aagctggccg aacggatcaa gcatggcggc
     4141 ttcggcaaca gccggatcga gggcctgccc gcggccgact gggtgct
//
LOCUS       DATNBV010000236         2602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_153110,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000236 DATNBV010000000
VERSION     DATNBV010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153110"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /gene="miaB"
                     /locus_tag="VN627_05715"
     CDS             <1..1068
                     /gene="miaB"
                     /locus_tag="VN627_05715"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXD02787.1"
                     /translation="AQAEGEEIMARAPAVKMVVGPQAYHRLPEMIAEATAGRRAVDTD
                     MPAQAKFDALPARRKVGPSAFLTVQEGCDKFCTYCVVPYTRGAEISRPFSAIVDEAKK
                     LIEAGARELTLIGQNVNAWEGEDGKGRAVGLDGLIRHLAEDPDLARIRYTTSHPNDMT
                     EGLIAAHVEVAKLMPFLHLPVQSGSNRILKAMNRSHTAESYLEILERVRAARGDIALS
                     GDFIVGFPGETEEDFAQTLSLVQSVGYAQCFSFKYSPRPGTPAATMDHQIAPDVMDER
                     LQRLQAALNASQHAFNAASVGKRCAVLVERKGRHAGQWLGKSPWLQSVHFEGAADIGD
                     LVEVELVAAGPNSLSGRLVGG"
     gene            1254..2291
                     /locus_tag="VN627_05720"
     CDS             1254..2291
                     /locus_tag="VN627_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXD02788.1"
                     /translation="MARKTARATEGMAHPWSSDTRRESPRRARAELEFDEPTVLGALF
                     GQFDSNLVLIENRLGVYIGARGNRIQIEGGEDAVARARDVLKGMHQRIMSGQEIDAGA
                     VESLIAMSNEPTLEGIITGGNDAPPIMIRTRRKTIVPRSATQIDYMRSLAKDDVIFAL
                     GPAGTGKTYVAVAQAVSQLMNGSVQRLILSRPAVEAGERLGFLPGDMKEKVDPYLRPL
                     YDALYDCMPPEQVERRMASGEIEVAPIAFMRGRTLADAFVILDEAQNTTPAQMKMFLT
                     RFGQNSRMVVCGDPRQVDIPGGDSFSGLADAVKRLEGVDGISVVRFNAADVVRHPIVG
                     RIVEAYEGKDS"
     gene            2288..2602
                     /locus_tag="VN627_05725"
     CDS             2288..2602
                     /locus_tag="VN627_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HXD02789.1"
                     /translation="MSAKGSDVAREHHVLIRHEVADYAAWKAVFDEAAGIRRSAGEIS
                     YQLLAYDDDAAHIVHFTRWTSLAAARAFFESDELIEIRRKAGVKAPEFIYLNAIEAGV
                     LA"
BASE COUNT          465 a          821 c          900 g          416 t
ORIGIN      
        1 gcccaggccg agggcgagga gatcatggcc cgcgcgccgg cggtaaagat ggtggtgggc
       61 ccgcaggcct atcaccgcct gcccgagatg atcgccgaag cgacggcggg gcgccgcgcg
      121 gtcgataccg acatgccggc ccaggccaag ttcgacgccc tgcccgcccg ccgcaaggtg
      181 gggccgagcg ccttcctgac ggttcaggaa gggtgcgaca agttctgcac ctattgcgtg
      241 gtgccctata cccgcggcgc cgaaatatcg cgtccattca gcgcgatcgt cgacgaagcg
      301 aagaagctga tcgaggccgg cgcgagggaa ctgacgctga tcggccagaa cgtgaacgcc
      361 tgggaaggcg aggatggcaa ggggcgcgcc gtggggctcg acgggctgat ccggcatctc
      421 gccgaggacc cggacctggc gcgcatccgc tataccacca gccaccccaa cgacatgacc
      481 gaagggctga tcgccgccca cgtcgaggtg gccaagctga tgccgttcct ccacctgccg
      541 gtgcagtctg gatcgaaccg catattgaag gcgatgaatc gctcgcacac ggctgaatcc
      601 tatctggaaa tccttgagcg tgtgcgtgcc gccaggggcg atatcgcgct gtcgggcgat
      661 ttcatcgtcg gcttcccggg cgagacggaa gaagactttg cccagacgct ttcgctcgtg
      721 caatcagtcg gttacgcgca gtgcttcagc ttcaagtaca gcccgcgccc aggcactccg
      781 gcggcgacca tggatcacca gatcgcgccc gatgtgatgg acgagcggct gcaacggctg
      841 caggccgcgc tcaatgccag ccagcatgcg ttcaatgcgg cctcggtggg caagcgctgc
      901 gcggtgctgg tcgagcgcaa ggggcggcat gccgggcaat ggctgggcaa gtcgccgtgg
      961 ctgcaatcgg tccacttcga gggcgcggcg gatatcgggg acctggtcga agtggaactg
     1021 gtggcggcgg ggccaaattc gctgagcggg cggttggtgg ggggctgagc ccgggcgccg
     1081 cgctgggctg cggcgttgcg ccgtggatgc tgaaacaagt tcagcatgac gagctggaga
     1141 ccggagttcg tttttccaca tcgccgcagc gcagcacggc ttgtttgtca tcatccgtgg
     1201 cctatctttc cctttgggaa tcgccctttc ggcgagcccg cagaaaggag cgcatggccc
     1261 gcaaaactgc tcgcgccacc gaagggatgg cgcatccctg gtcgtccgac acccgccgcg
     1321 agagcccgcg gcgcgcccgg gcggagctgg aattcgacga gcccaccgtg ctgggcgccc
     1381 tgttcgggca gttcgattcc aacctcgtgc tgatcgagaa ccgcctgggc gtctatatcg
     1441 gcgcgcgcgg caaccggatc cagatcgagg gcggcgagga tgcagtggcc cgcgcgcgcg
     1501 acgtgctcaa gggcatgcac cagcggatca tgagcgggca ggagatcgac gccggcgcgg
     1561 tagaatcgct catcgccatg tcgaacgagc cgacgctcga agggatcatc accggcggca
     1621 acgatgcccc gccgatcatg atccgcaccc ggcgcaagac catcgtgccg cgttccgcga
     1681 cgcagatcga ctacatgcgc agcctcgcca aggacgacgt gatcttcgcg ctgggcccgg
     1741 ccggcaccgg caagacctat gtcgcggtgg cccaggccgt atcgcagctg atgaacggct
     1801 cggtccagcg gctgatcctc tcgcggcccg cggtggaagc gggcgagcgg ctggggttcc
     1861 tgccgggcga catgaaggag aaggtcgatc cctacctgcg cccgctttac gatgcgctct
     1921 acgattgcat gccgcccgag caggtggagc ggcgcatggc cagcggcgag atcgaggtcg
     1981 cgccgatcgc cttcatgcgc gggcgcaccc tggccgatgc cttcgtgatc ctcgacgagg
     2041 cgcagaacac cacgccggcc caaatgaaga tgttcctcac ccgcttcggc cagaacagcc
     2101 ggatggtagt ctgcggcgac ccgcggcagg tagacatccc gggcggcgat tcgttcagcg
     2161 ggctggccga tgcggtcaag cggctggagg gcgtggatgg catctcggtg gtgcgcttca
     2221 acgcggccga cgtggtgcgc catccgatcg tcgggcggat cgtcgaggcc tatgagggca
     2281 aggacagttg agcgcaaaag ggagcgatgt cgcccgcgag catcacgtcc tgatccgtca
     2341 cgaagttgca gattacgccg cgtggaaagc ggtcttcgac gaagctgccg ggatcaggcg
     2401 atcagccggc gagatatcgt accagttgct ggcctatgac gacgatgccg cgcacatcgt
     2461 ccatttcacc cgctggacca gcctggccgc ggcgcgcgcg tttttcgaat cggatgaact
     2521 gatcgagata cgccgcaagg ccggggttaa ggcacccgaa ttcatttatc tcaacgccat
     2581 agaggccggg gttctggcat ag
//
LOCUS       DATNBV010000237         2155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4280719,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000237 DATNBV010000000
VERSION     DATNBV010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4280719"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VN627_05730"
     CDS             complement(<1..511)
                     /locus_tag="VN627_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HXD02790.1"
                     /translation="MGQDDTSALAGDLLPLSLSQQEVWLDQRAWPGSTHLNIGGAGYI
                     DGPFDLALFRQALAHLVAESEALRLVPLVEGGQRLLAAYEGPLKVVDLSAAADPLAAM
                     HTWWQNWMVEPFAFDGTPPWRFALLRYSDRLHGLTIQFHHLIMDGWGTSQVMQRWAAI
                     YNALVRGEQP"
     gene            complement(484..>2155)
                     /locus_tag="VN627_05735"
     CDS             complement(484..>2155)
                     /locus_tag="VN627_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXD02791.1"
                     /translation="LQSVKGGCPVLHEGENSESMLILVSGRLRVSRRNADGSLLLYNE
                     ICPGETVGETGMVLQQARFADVTALRDSTIALLRRERFEELLGRHPVPLNRVFAQAVY
                     HQLRHTPQLADRQRAHTIVIVPLRDHHLGAEVASGLADALEAMGRTRKVSQEGLNLLE
                     LDALESEFEFIVLETDASDSERTHLAFRQADQVVFVAAEDEPNDLAQIECGLAREAGF
                     LMKRKHLALCHKADADAPGDMPAWQRGRDIERVYPLRAGRQRDYARLARFLTSSAVGL
                     VLGGGGARGFSHLGVLRALEENGIPVDIIGGNSMGALIGAQYACGVPIDEIRQRTQRF
                     ALGGERPTLPLISLLSGKRLERDLRRMFGDIRIEQLWRPFFAAACNLSQACTTVQDSG
                     LLWRAVLASNSPAGLLPPVIYNGDLLVDGAILDNVPVSAMRGRLGTPLERRRGNGTII
                     AVDVDVRENLCVDPEMSRLSVWRTLKGYLRAAGEGAPSIGAILYRAGHIGGMHERART
                     MAMADFCLEPPVAEFPLMAYRRAAEIVDTGYRHAMEEIARWDKTTLRR"
BASE COUNT          401 a          736 c          670 g          348 t
ORIGIN      
        1 tcggctgttc gccacgaacc agggcgttgt agatggctgc ccagcgctgc atgacctgcg
       61 aggtgcccca gccgtccatg atcaggtggt gaaactggat ggtcagccca tgcaggcggt
      121 cgctgtagcg cagcagggca aagcgccagg ggggcgttcc atcgaaggcg aacggttcga
      181 ccatccagtt ctgccaccag gtatgcatcg ccgccagcgg atcggcggcc gccgagagat
      241 cgaccacttt cagcgggcct tcgtaagcgg cgagcaggcg ttgccccccc tcgaccagcg
      301 gaaccagccg taaggcctcg ctttcggcga ccagatgcgc caacgcctga cgaaacaacg
      361 ccaagtcgaa cgggccatcg atataccccg ccccaccgat gttgagatgc gtgcttcccg
      421 gccaggcccg ctggtcgagc cagacttcct gctggctcag ggaaagcgga aggagatcgc
      481 ccgctagcgc cgaagtgtcg tcttgtccca tcgggcaatc tcttccatgg cgtggcggta
      541 accggtatcg acaatttcgg cggcacggcg gtaagccatc agcgggaatt ccgccaccgg
      601 cggctcaagg cagaagtcag ccatcgccat cgtccttgcc cgctcgtgca tgccgccgat
      661 atgccccgcc cggtagagga tggccccgat ggagggagcc ccctccccgg ccgcccgcaa
      721 atagcctttc aaggtacgcc agaccgaaag ccgcgacatt tcgggatcga cgcacaggtt
      781 ctcgcgaaca tcgacatcga ccgcgatgat cgtgccgttg ccccgccgcc gttcgagcgg
      841 cgtccccagc cgtccacgca tggcgcttac cgggacgttg tcgagaattg ccccgtcgac
      901 cagcaggtca ccgttgtaga tcaccggcgg cagcagcccg gccggcgagt tgctggccaa
      961 taccgcccgc cagagcaggc cgctgtcctg gaccgtcgtg caggcctgac tgagattgca
     1021 ggccgccgca aagaagggac gccacagttg ttcgatacgg atatcgccga acatccggcg
     1081 cagatcgcgt tccaggcgct tgccggaaag cagggagatc agcggcaggg tcgggcgctc
     1141 gccacccaaa gcgaagcgct gcgtccgctg gcgaatctcg tcgatcggca cgccgcaagc
     1201 gtactgcgcc ccgatcagtg cgcccatgct gttgccgccg atgatatcga cgggaatccc
     1261 gttttcctcg agcgcccgca gcaccccgag atgcgagaat ccgcgcgcgc cgccgccgcc
     1321 cagcaccagg cccaccgcac tgctggtcag gaaacgcgcc aaacgggcat agtcccgctg
     1381 gcgtcccgcg cgcagcggat aaacccgttc tatgtcgcgc ccgcgctgcc aggccggcat
     1441 atccccggga gcatcggcgt cggccttgtg gcaaagcgcc agatgcttgc gtttcatcag
     1501 gaagccagcc tcgcgggcga gaccgcactc gatctgggcc aagtcgttcg gctcatcttc
     1561 ggcggcgacg aacaccacct gatcggcctg ccgaaaggcc agatgggtac gctccgaatc
     1621 gctggcgtcc gtctccagca cgataaattc gaactcgctc tccagggcgt ccagctcaag
     1681 cagattgagg ccttcctggc tgaccttccg ggttcgcccc atcgcctcca gcgcatccgc
     1741 cagcccactg gcaacctctg cgcccagatg atgatcgcgg agcggcacga taacgatggt
     1801 atgggcacgt tgccggtccg ccagttgcgg ggtgtggcgc aactggtgat acaccgcctg
     1861 ggcaaaaacg cggttcagcg gcactgggtg gcggccgaga agctcctcga agcgttctcg
     1921 gcgaagcaag gcaatggtcg agtcgcgcag cgccgtgaca tcggcaaatc gcgcctgctg
     1981 caggaccatg ccggtttcgc cgaccgtctc gcccggacag atttcgttgt aaagcaggag
     2041 cgaaccgtcg gcattgcgcc gcgacacccg caagcggccg ctgaccagaa tcagcatgct
     2101 ttcggagttt tcgccttcat ggagaaccgg gcatccgccc ttgaccgact gcagt
//
LOCUS       DATNBV010000238         4545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2696222,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000238 DATNBV010000000
VERSION     DATNBV010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2696222"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="VN627_05740"
     CDS             complement(<1..1307)
                     /locus_tag="VN627_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXD02792.1"
                     /translation="MGPLRLVSLGTIAAMLLPSPAGAAQAGPTTATPLAVTPAPAQQA
                     PVRPNVLVWMMDDVGFAQLSSYGGLVPTPNIDRVASRGLRYDNYHTAAVCSASRAALL
                     TGRMPHSVNMGGHAAIPMPFPGYDGQVPAGAGTVAENLKQAGYATFAVGKWDHLPTSE
                     VSPAGPFTHWPLQQGFERYYGYLAADTDNWHPNLVQDNAPAPTPQTPGYHLSADLADR
                     AIAMIRSRDGRAPAAPFFLYWATGAAHAPLHAPREWIERFRGKFDMGWDQAREDILRR
                     QIAMGLQPRGTRLSPRPPAMPAWSSLSADQKRMYARQMEVFAAALAYADAQFGRILDE
                     LERRGELDNTMVVVVSDNGASGEGGEEGKLTEAFLAAQREATLAENLKFYDRWGGPET
                     YPTYNVGWAVAGDTPFRYYKQTTYEGGQRVPLVVSWPRAIAATG"
     gene            1527..4106
                     /gene="clpB"
                     /locus_tag="VN627_05745"
     CDS             1527..4106
                     /gene="clpB"
                     /locus_tag="VN627_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HXD02793.1"
                     /translation="MNLEKFTDRAKGFLQAAQTVAIRNSHQRIAPEHILKALLDDDQG
                     MAAQLIQRAGGNAKFADDEVQRALAKMPAVSGGGAQQTPGLDNDAVRVLDQAEQLATK
                     AGDSFVPVQRILQALALSNGAAGQALKAANVDAKALEAAIQEATGGRTADSASAEESY
                     DALKKYARDLTAAAREGKLDPVIGRDEEIRRTVQILARRTKNNPALIGEPGVGKTAIA
                     EGLALRIANGDVPDSLKDRSLMALDMGSLIAGAKYRGEFEERLKAVLDEVKGAEGQII
                     LFIDEMHTLIGAGKSEGAMDAGNLLKPALARGELHCIGATTLDEYQKYVEKDPALQRR
                     FQPVFVGEPTVEDTISILRGLKDRYELHHGVRITDGAIVAAATLSNRYIADRFLPDKA
                     IDLMDEAASRIRMEVESKPEEIEKLDRRIIQLKIEEQALAKESDQPSKDRLAALREEL
                     ANLEQQNAELTTRWQNERDKIAAEGKIKEQLDAARVELEQAQRQGDYAKAGELSYGRI
                     PELEQALAEAQNAGTDGAMLREEVTADDIAGVVSRWTGVPVDRMMEGEREKLLRMEEV
                     IGARVIGQKDAVTAVSKAVRRARAGLQDPNRPLGSFLFLGPTGVGKTELTKALAGFLF
                     DDDNAMVRIDMSEFMEKHAVSRLIGAPPGYVGYDEGGVLTEAVRRRPYQVVLFDEVEK
                     AHPDVFNVLLQVLDDGRLTDGQGRVVDFTNTLIILTSNLGSQYLAGLEEGQDVSAVEP
                     QVMEVVRGHFRPEFLNRLDEIILFHRLGQEHMAPIVDLQVARVQKLLKDRKITLDLTD
                     AARRWLGRVGYDPVYGARPLKRAVQRYLQDPLAEKLLGGEIPDGSTVRIEEGEGQLAI
                     TVE"
     gene            complement(4259..>4545)
                     /locus_tag="VN627_05750"
     CDS             complement(4259..>4545)
                     /locus_tag="VN627_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02794.1"
                     /translation="FSKTGRFAAATMASNPTITAPEYRQYKARFVHDLYLASDVTDAF
                     QIYGGVNNLFDQKPDIATLGDSIQSPTYPVSAVGRYLYVGARVKLGELFQ"
BASE COUNT          812 a         1403 c         1629 g          701 t
ORIGIN      
        1 cccgtggcgg caatcgcgcg cggccaggac accacgagcg gcacgcgctg gccgccttca
       61 taggtggtct gcttgtagta gcggaacggc gtgtcgccgg cgacagccca gccgacgtta
      121 taggtgggat aggtctcggg cccgccccag cgatcataga acttgagatt ttccgccagc
      181 gtggcttcgc gctgggcggc gaggaaagcc tcggtcaact tcccttcctc gccgccttcg
      241 ccgctagcgc cgttgtcgga gacgaccacc accatggtgt tgtcgagctc gccgcgcctt
      301 tccagctcat cgaggatccg gccgaactgc gcgtcggcat aggccaaggc agctgcgaag
      361 acttccattt gccgggcata catgcgcttc tggtcggccg agaggctgga ccatgccggc
      421 atggccgggg ggcggggcga gaggcgggtg cccctgggct gcaggcccat ggcgatctga
      481 cggcgcagga tgtcctcgcg ggcctggtcc cagcccatgt cgaacttgcc gcggaaccgc
      541 tcgatccatt cgcgcggggc atgcagcggg gcatgggcgg cgccggtcgc ccagtacagg
      601 aagaacggag cagccggagc ccggccgtcg cgagaacgga tcatcgcgat tgcccggtcg
      661 gcgaggtcgg cgctgagatg atagccgggc gtctgcgggg ttggcgccgg cgcgttgtcc
      721 tgaacgagat tggggtgcca gttgtcagta tcggcagcga gatacccgta atagcgctca
      781 aacccctgct ggagcggcca gtgcgtgaac gggccggccg gggagacctc gctcgtcggc
      841 agatggtccc acttgcccac tgcgaaggtg gcatagccgg cctgcttgag gttttcggcc
      901 accgtgccgg cgccggccgg aacctgtccg tcatagccgg ggaagggcat gggaatggcc
      961 gcgtgaccgc ccatgttgac gctgtgcggc atccgcccgg tcaacagggc ggcgcggctg
     1021 gccgagcaga ccgcggcggt gtggtagttg tcgtagcgca gcccgcggct ggcgacccga
     1081 tcgatgttgg gcgtgggaac gagcccgccg tagctggaaa gctgggcgaa gccgacgtcg
     1141 tccatcatcc acaccagcac gttgggccgg actggcgcct gctgtgcggg agcaggggtg
     1201 acggccagcg gggtggcggt ggtcggcccg gcctgcgctg ctccggcggg ggagggcagg
     1261 agcatggcgg cgatcgtccc cagtgaaacc agacgcagcg gacccatcgg tgatccctcc
     1321 tcggtgtgcc ggtttcgccc gactgcatga ttggctgccg atggtgcctg tgggcggcta
     1381 ttgcgtccaa tgcaaaacat tggcggaatc tatgcgcaca ggacatgcac tgtctgtgcc
     1441 gccgagtttg cgcgtttttt gatctgtctc cttgtgtgtc caaaaagtca cactaccttt
     1501 cgctccaaca ggaggcgaga gcacccatga atctcgaaaa gtttaccgac cgcgccaagg
     1561 gtttcctgca ggctgcgcag accgtcgcga tccgcaacag ccatcagcgg attgcgcccg
     1621 agcatatcct caaggccttg ctcgacgacg accagggcat ggccgcgcaa ctgatccagc
     1681 gggccggcgg caatgcgaaa tttgccgatg acgaggtgca gcgggcgctg gcgaagatgc
     1741 cggcggtttc gggcggcggg gcgcagcaaa cgcccgggct ggataacgat gccgtgcgcg
     1801 tgctcgacca ggccgagcag ttggcgacca aggcgggcga cagcttcgtg ccggtgcagc
     1861 gcattctcca ggcgctggcg ctgagcaatg gcgcggcggg gcaggcgctg aaggcggcga
     1921 atgtcgatgc caaggccctc gaggcggcga tccaggaagc aaccgggggc cgcacggcgg
     1981 attcggccag cgccgaggaa agctatgatg cgctcaagaa gtatgcccgc gatctcaccg
     2041 ccgcggcgcg cgagggcaag ctcgacccgg tgatcgggcg cgacgaggaa atccgccgca
     2101 cggtccagat cctcgcccgg cgcaccaaga acaacccggc gctgatcggc gagccgggcg
     2161 tgggcaagac cgcgattgcg gagggcctcg cgctgcgcat cgcgaatggc gacgtgcccg
     2221 acagcctcaa ggaccgcagc ctgatggcgc tcgacatggg cagcctgatc gcgggcgcga
     2281 agtatcgcgg cgaattcgag gagcggctca aggccgtgct cgacgaggta aagggcgccg
     2341 aaggccagat catcctgttc atcgacgaga tgcacacgct gatcggcgcg ggcaaatcgg
     2401 aaggcgcgat ggatgcgggc aacctgctca agccagcgct cgcccgcggc gaactgcact
     2461 gcatcggtgc gaccacgctg gatgagtacc agaagtatgt tgaaaaggac ccggcgctgc
     2521 agcggcggtt ccagccagtg ttcgtcggcg agccgaccgt ggaggacacg atctcgatcc
     2581 tgcgcggcct taaggaccgc tacgagctgc accacggcgt gcggatcacc gacggcgcga
     2641 tcgtggcggc ggcgacgctg tcgaaccgct acattgccga ccgcttcctg cccgacaagg
     2701 cgatcgacct catggacgag gccgccagtc gcatccgcat ggaggtggaa agcaagcccg
     2761 aggagatcga gaagctcgac cggcggatca tccagctcaa gatcgaggag caggcgctgg
     2821 ccaaggaaag cgaccagcct tcgaaggatc ggctcgctgc tctccgcgaa gagctggcca
     2881 accttgagca gcagaacgcc gagctgacca cccgctggca gaacgagcgc gacaagatcg
     2941 cggcggaagg caagatcaag gagcagctcg acgccgcgcg cgtggaactg gagcaggccc
     3001 agcgccaggg cgactatgcc aaggcgggtg agctttccta tggtcggatt cccgagttgg
     3061 agcaggccct tgccgaagcg cagaacgccg gcaccgatgg cgccatgctg cgcgaggaag
     3121 tgaccgcgga cgacattgcc ggcgtcgtca gccggtggac cggggtgccg gtcgaccgga
     3181 tgatggaggg cgagcgcgag aagctgctgc gcatggaaga ggtcatcggc gcgcgagtca
     3241 tcggccagaa ggatgcggtc accgccgtgt cgaaggcggt gcggcgcgcg cgggccggct
     3301 tgcaggaccc gaaccggccg ctgggctcgt tcctgttcct cgggcccacg ggcgtgggca
     3361 agaccgagct gaccaaggcg ctggccggct tcctgttcga tgacgacaac gcgatggtcc
     3421 gcatcgacat gtcggagttc atggagaagc acgcggtcag ccgcctgatc ggcgcgcctc
     3481 cgggctatgt cggctatgac gagggcggcg tcctcaccga agccgtgcgg cggcggccct
     3541 accaggtcgt cctgttcgac gaggtcgaga aggcccaccc ggacgtgttc aacgtactgc
     3601 tccaggtgct cgacgatggc cgtctgaccg acgggcaggg ccgggtggtg gacttcacca
     3661 acaccctgat catcctcacc tccaacctcg gcagccagta tcttgccggg ctcgaggaag
     3721 ggcaggacgt gagcgcggtc gagccgcagg tgatggaagt ggtgcgcggg catttccggc
     3781 ccgagttcct caaccggctc gacgagatca tcctgtttca tcgcctcggc caagagcaca
     3841 tggcgccgat cgtggatctc caggtggcgc gggtgcagaa gctgctcaag gaccggaaga
     3901 tcacgcttga ccttaccgac gccgcccggc gctggctggg gcgggtgggc tacgatccgg
     3961 tctatggcgc gcggccgctc aagcgggcgg ttcagcggta cctgcaggat ccgctggcgg
     4021 agaagctgct gggtggggag atccccgacg gcagcaccgt gcggatcgag gagggcgagg
     4081 gacagctggc gataaccgtg gagtgacccc gcgcccctga acggacgaag ggccggagag
     4141 cgctaacgct ccccggcccc tcttttggtg cagcaatccg cggaggctca gaacgcggcg
     4201 ctggcaccaa ccttgaaatc accggcgagg gcggatgtca tgccccggta gtcgccgttc
     4261 actggaacag ttccccgagc ttgaccctgg cgccgacata gagatagcgg ccgaccgcgc
     4321 tgaccggata ggtcggagac tggatcgaat ctcccagcgt tgcgatgtcg ggcttctggt
     4381 cgaacaggtt gtttaccccg ccatagatct ggaaggcatc ggtcacgtcg gacgcaaggt
     4441 agagatcgtg gacgaagcgc gccttgtact ggcggtattc cggtgccgtg atggtcgggt
     4501 tggaggccat ggtggcagcg gcgaagcgac ctgttttcga gaagt
//
LOCUS       DATNBV010000239         3454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3338588,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000239 DATNBV010000000
VERSION     DATNBV010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3338588"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VN627_05755"
     CDS             complement(<1..643)
                     /locus_tag="VN627_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD02795.1"
                     /translation="MATTAKPPRPKGLAVLRVALQSRKSATMLAFGFASGLPYALLIG
                     TLNAWLGEVGVKLATIGVLSWIGLSYSFKFLWSPLVDRLPLPGLERLGRRKSWIFLCQ
                     AVLVLAFAGLSFTDPAHAIGTFALFAFLAALASATQDVAVDAWRIDVADEQAPVELLS
                     AIYQFGYRIASIVGGALALVLAARMSWGSVYLAMGGLLLLVAFITLRAPDTPRP"
     gene            843..1625
                     /locus_tag="VN627_05760"
                     /pseudo
     CDS             843..1625
                     /locus_tag="VN627_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            2126..2686
                     /gene="rsmD"
                     /locus_tag="VN627_05765"
     CDS             2126..2686
                     /gene="rsmD"
                     /locus_tag="VN627_05765"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HXD02796.1"
                     /translation="MSLRIIAGQWRGRKLVAPEGDTTRPTADRTRETLFSMLQSRLGS
                     FEGLAVADLFAGSGALGLEALSRGSASCLFVEQDPAAVRAIRANLAALRAQAQGDVRA
                     SSVLSLGPARAALDLVFLDPPYHTGAGAVALDKLVRLGWIGPATWVSLETAADEEPKV
                     RGLDAVAERRVGKARITLLRLAGQPS"
     gene            complement(2674..>3454)
                     /locus_tag="VN627_05770"
     CDS             complement(2674..>3454)
                     /locus_tag="VN627_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD02797.1"
                     /translation="FLAANGLFLILGATLSEADFAAWGWRIPFLASAVLVGLGLWVRL
                     RIGETMAFKEALAHEAPVPVPLARLISRHGGAVLAGSAGVVACFAIFYLATAFALAQG
                     AGPLGYAREEFLAVQLGANLFLALGIIAAALHADRTSPRRTLTFGALLAVVVGVLFGG
                     GLASGSLGVVFLTLAISLFVMGLCYGPLGAWLPTLYPPLVRYSGISVAFNVGGIVGGA
                     LTPLAAQAMAGAGQVHFVGLLIAAAGVLTLIGVASARPHEG"
BASE COUNT          547 a         1126 c         1374 g          407 t
ORIGIN      
        1 ccgggcgcgg cgtatcaggc gcgcgcaggg tgatgaaggc gaccagcagt agcaggccgc
       61 ccatggcgag gtagaccgag ccccagctca tccgcgccgc cagcaccagc gccagtgccc
      121 cgccgacgat cgaggcgatg cggtagccga actggtagat cgcgctcagt aattcgaccg
      181 gggcctgttc atcggccacg tcgatccgcc aggcatcgac ggcgacgtcc tgcgtggccg
      241 aggccagcgc ggcgaggaag gcgaagaggg cgaaggtgcc gatggcatgg gccggatcgg
      301 tgaaggaaag cccggcgaag gcgaggacga gcacggcctg gcacaggaag atccacgact
      361 tgcggcggcc gagccgttca aggccgggga gcggcaggcg atcgaccagc ggcgaccaca
      421 ggaacttgaa ggaataggac aggccgatcc acgagagcac gccgatggtg gcgagcttca
      481 ccccgacctc gcccagccag gcgttgagcg tgccgatcag cagggcatag ggcaggcccg
      541 aggcgaagcc aaaggccagc atggtggccg acttgcgcga ttgcagcgca acgcgcagca
      601 ccgcgaggcc cttgggccgg ggcggcttgg cagtggtggc catctcatgt ccctccggat
      661 gggctttatc acaaaccgtt cgtgtcgagc gaagtcgaga cactggcgca tggtgtctcg
      721 acttcgctcg acacgaacgg ctggtgggcg gagactagcg ggatcgtggg ccttggccag
      781 agccatggcc ctcgcctccc ccgctttttg acgcatcccg gccgcgccgc tacagcgccg
      841 cgatgaacga ctcccctgac agttccggcg ccccatccgc cgcccgtacc ggcgaacgca
      901 ttgccaagct gctggcccgc gcgggcgtgg ccagccggcg cgaggtggag cggatgattg
      961 cggaagggcg ggtgcggctg aacgacgtgg tggtggagac gccggcgacg gtcctccctt
     1021 ccttgcacgg cgtgactgtc gacgggcggc cggtgcgcgc ggccgaggcg acgcggctgt
     1081 tcctgtttca caagcccggc gggctgatca ccgccgagcg cgacccgagc ggcaagccga
     1141 cgatctatac ggcgctgagg aatgccctgc cgccgggcac gccgcgcgtg atgccggtgg
     1201 gccggctcga cctcaacacc gaggggctgc tgctgctgac caacgacggc gagctgaagc
     1261 gggcgcttga gctgccggcc tcgggcgtgc cgcgcaccta ccgggcgcgg acctttggcg
     1321 acattaccca ggcccggctg gaagagctga tcgagggcat cgagatcggg ggcatccgct
     1381 atggcgcgat cgatgcgaac atggagcggc gcacgggccg caaccagtgg atcgaactga
     1441 cgctggcgga aggcaagaac cgcgaggtgc gccgggtgct cgagcatctg gggctggaag
     1501 tgagccgcct gctgcgcacg gcctacgggc cgtatcacct gggcgacctg ccgcgcggca
     1561 gcgcctggga ggtgccgcag aaaaccgtgg aggtattccg caaggtgctg ctggaaggcg
     1621 gcaaggcgct ggccccggtg tttgcgccgc cgatggaggc acaggctcgg ccggccaggt
     1681 tgaatgatgc ccctgccccc gcacctgccc cgccgcgcgg gacgcggggg gcggcgcggc
     1741 aggctggcgg gccggcgggt gctgaccggc ggcctgcgcg gcgcggggct ggcccgcgcg
     1801 atggagtctc tcgcggtggt acgggggcgg atcccacggc gcgcatcggc aagcccgccg
     1861 agcgcgggac gcgcggcggt gcggcggcca ggccggagcg gagcgcccgg ccggagcgca
     1921 ccgagcgccc tgctgaacgg agcgcccggc cccggcggca gggggaggag cgcgacgagc
     1981 tgtttacccg ccgctcggaa gcgggcggcg gccgggcggc accggagcgt gggcgcgggg
     2041 cgcgcaagcc ctccagaagt gggccggcgc ggggcgggcc tgagggtccg cggcctgggc
     2101 cgggcaagcc gcgggggccc cgccggtgag cctcaggatt attgccgggc agtggcgcgg
     2161 gcgcaagctg gtggcgccgg aaggggatac tacccggcca accgctgacc gcacgcgcga
     2221 gacgttgttt tcgatgctgc agagccgcct cgggagcttc gaggggctgg ccgtggccga
     2281 cctgtttgcg gggtctggcg cgctcgggct ggaggcgctg tcgcgaggga gcgcttcatg
     2341 cctgttcgtg gagcaggacc cggcggcggt ccgggcgatc cgggccaatc ttgccgcgct
     2401 gcgggcccag gcgcagggcg atgtgcgagc ctcttccgtg ctgtcgctgg ggccggccag
     2461 ggcggcgctt gacctcgttt tcctcgaccc gccctatcac accggcgcgg gggccgtggc
     2521 gctcgacaag ctggtgcggc tgggatggat cggcccggcc acctgggtga gcctggagac
     2581 ggccgccgac gaggagccga aggtgcgcgg gctcgacgcc gtggccgagc ggcgcgtggg
     2641 caaggcgcgg attacgctgc tgcgcctggc tggtcagccc tcgtgagggc gcgctgaggc
     2701 gacgccgatg agggtgagca cgccggccgc tgcgatcagc aggccgacga agtggacctg
     2761 gccggcgccg gccatggctt gcgccgccag cggggtcagc gcgccgccga cgatgccgcc
     2821 gacgttgaag gcgaccgaga tccccgaata gcgcaccagc ggcgggtaga gcgtgggcag
     2881 ccaggcgccg agcgggccgt agcacaggcc catgacgaac agcgatatgg ccagcgtcag
     2941 gaagaccacg ccgagcgagc ccgaggcaag gccgccgccg aacagcacgc cgacgaccac
     3001 cgcgagcagg gcgccgaagg tgagcgtgcg gcgcgggctg gtgcggtcgg catggagcgc
     3061 ggcggcgata atgccgaggg cgaggaacag gttcgcgccg agctggacgg cgaggaactc
     3121 ctcgcgcgcg taaccgaggg ggccggcgcc ctgagcgagg gcgaaagccg ttgcgagata
     3181 gaagatggcg aagcaggcga cgacgcccgc gctcccggcg aggaccgcgc cgccgtggcg
     3241 cgagatgagg cgggccagcg ggaccgggac cggggcttca tgggcaagtg cctccttgaa
     3301 ggccatggtc tcaccaatgc gcaggcgcac ccacaggccg aggccgacca gcacggcgct
     3361 ggcgaggaac gggatgcgcc agccccaggc ggcgaaatcg gcctccgaca gggtcgcccc
     3421 gaggatcagg aacaggccgt tggcggcaag gaag
//
LOCUS       DATNBV010000240        11321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3338856,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000240 DATNBV010000000
VERSION     DATNBV010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11321
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3338856"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..979)
                     /locus_tag="VN627_05775"
     CDS             complement(65..979)
                     /locus_tag="VN627_05775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HXD02798.1"
                     /translation="MKLTFPMPHLMEIAAVTQPWELTVTGPDQLRLARRAEELGYDMI
                     TVPEHYAVPPTDHHTGAFWLHATTAQAALAGATSRIVLNSGITILPFSNPIVHAKALA
                     TADWLSGGRIAVTFGLGSIEDEYPYFGVPWRERGAIADDYLAAIVALWTEERPTYHGK
                     YVQFEDIGFQPKPVQKPHLPIWIGGDSPAALRRVARFGSGWIVTFRTRPDEVPDRIEA
                     IKSAPAWTDRPLEVTYSLANLLLTEGHGASGASGPDPTSAQQLVDALGHLRECGITMS
                     SVPMPPVASVEAYLDHAQWVMEEVRPHV"
     gene            1172..2590
                     /locus_tag="VN627_05780"
     CDS             1172..2590
                     /locus_tag="VN627_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD02799.1"
                     /translation="MGLWPDQQTEHHHADGLPVPRRYFAAITIWLAISMSVLDAAIAN
                     IALPTIAMELHAAPAAAIWVINAYQLATTMLLLPLAAMGDRIGHARVYIGGLALFVIG
                     SLLCALAGSLGALIAARVVQGMGAAGVMSMNAALVRATYPSDMLGRAMGYNALVLSVA
                     AAVGPSLASGILAVATWPWLFAINVPVGLASLAVGWHCLPHAPGHGRAPDFTAAALSA
                     AMLGLVVYGAESFAREGRTSGLVLLVIGLACGAGLVMWERGKAHPLFPTDLLARPVFA
                     LSIMASTTSFAAQTLAYVSIPFMLQQVLGRGVVESGLLMTPWPIAAGTCAFIAGRLAD
                     RWPAGLLGGAGLLMLAGGLFALSRLGAHPSDFDLVWRMALCGAGFGTFQTPNNRTIIG
                     SAPRERAGAAGGMLATARLLGQTSGAVAVAVGFHRMGLGSSPFLLSCGAVAAVVAAGI
                     SLVRLRVPAPEGLPGARDVVAE"
     gene            2659..3429
                     /locus_tag="VN627_05785"
     CDS             2659..3429
                     /locus_tag="VN627_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HXD02800.1"
                     /translation="MSLPPDRLDRFARHIVLPEIGGAGQVALARANVVLVGCGGIGSP
                     ALQYLAAAGVGQLTLIDDDVVEASNLQRQTIFSTGDIGRGKAAAAANWVRHFDLALEV
                     HARQARIEAGNAGELIAGADVVLDGCDNFATRLAVSDACVKAGVPLTSAALGRFQGQV
                     GNFAGHLPGEPCYRCWVGDAFDAEDCDTCAADGVLGAMAGMVGCMGAMAAIRVLLEGH
                     AALGDPQWGKLQLLDGLAPSLRTIRVPKDETCRGCRAR"
     gene            complement(3568..4269)
                     /locus_tag="VN627_05790"
     CDS             complement(3568..4269)
                     /locus_tag="VN627_05790"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HXD02801.1"
                     /translation="MAQYRNIVILTGAGVSAESGIDTFRSEGGLWEQHRVEDVATPEA
                     FARDPDLVLRFYDMRRAAIQTKQPNAAHHALARLDSTWPGNLLIVTQNVDDLHERAGA
                     RRVHHMHGEHLTAWCTACDRRPRWTGPLIERPPCPLCGASALRPDVVWFGEMPYGMDR
                     IHAALREADLFVSIGTSGAVYPAAGFVRSARELGAATLELNLERSRGSAWFEETRLGP
                     ATELVPGWVEEVLGC"
     gene            4305..5033
                     /gene="dapB"
                     /locus_tag="VN627_05795"
     CDS             4305..5033
                     /gene="dapB"
                     /locus_tag="VN627_05795"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HXD02802.1"
                     /translation="MSRIGIIGSEGRMGKALAEAVAAAGEVLAGGIDKGGDPVALAAA
                     CDVLIDFSSPHALEANLDAAAGAGIPILVGTTGLEERHHWLVDAAAQKTAVLQTGNTS
                     LGVNMLAHLVREAAARLGEDWDIEIVETHHRMKVDAPSGTALLLGEAAAEGREIRLAD
                     NAVRGRDGITGARESGTIGFASLRGGTVAGDHTVYFLADNERIALSHLAENRLIFARG
                     AVRAAQWLVGKAPARYRMPEVLGL"
     gene            5030..5752
                     /gene="nth"
                     /locus_tag="VN627_05800"
     CDS             5030..5752
                     /gene="nth"
                     /locus_tag="VN627_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXD02803.1"
                     /translation="MKKDDIFEFFRRLAEANPSPETELEFGNVYQLLVAVTLSAQATD
                     VGVNKATRALFRAVKTPAQMVALGDEGLREQIKTIGLFNTKAKNVIALSEILVRDHGG
                     EVPNDRDALTALPGVGRKTANVVLNCAFGAETFAVDTHIFRVGNRTGLARGKTPLAVE
                     LQLEKKVPQPFRLGAHHWLILHGRYVCKARTPECWRCPVVDLCGYKAKVLEKGAGPAA
                     KNVRERSPRKSVPGTRLPKGDA"
     gene            5752..6165
                     /locus_tag="VN627_05805"
     CDS             5752..6165
                     /locus_tag="VN627_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02804.1"
                     /translation="MRLGSFRIGLAVAASCAALAGCYGYERGPDRAGPVDRIPAAAVT
                     GPDEACIPVHQFSQTVVRDGRTIDFLSSGRRGWRNVLPSDCPGLASERAFSFSTSLSQ
                     LCSTDVIRVLQNYGGGLQPGATCGLGRFTPIELRR"
     gene            complement(6306..7616)
                     /locus_tag="VN627_05810"
     CDS             complement(6306..7616)
                     /locus_tag="VN627_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HXD02805.1"
                     /translation="MTGKLTIRILIGLVLGIITGVAVNLWFPAGDPLQAQVADYLKLL
                     PDVFLHLIKMIIAPLVFATLVTGIAGMGDSSALGRIGGRALAWFITASLISILVGLLL
                     VNFFQPGVGLQFKAGGDIGQLATSDFTFRHFVLEVFPTSMVDAMAKNNVLQILVFSLF
                     FGLALSALRERGAILVEGLDALAQVMMRATDYVMRFAPLAVFGAMASVIALRGFGIVL
                     TYAALMSEFYFGLLILWTILFGLAALVLKKRAVTLARYIREPVILAFSTASSESALPR
                     LFEQLDRFGVPRRISGFVLPLGYSFNLDGSMMYTSFATLFIAQAYGIHLSAGQQIAML
                     LTLMVTSKGIAAVPRASLVVIAATLTQFGLPVEGIALILGVDTFLDLGRTATNVVGNA
                     VATAVITKWEGMLQPLRDPDGEGPEAPHDTPEHGRKGLNLDPQA"
     gene            complement(7838..8989)
                     /gene="dapE"
                     /locus_tag="VN627_05815"
     CDS             complement(7838..8989)
                     /gene="dapE"
                     /locus_tag="VN627_05815"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HXD02806.1"
                     /translation="MSQLPPVADLAEALIACPSVTPATGAVFDCLEGQLAPLGFEVRR
                     MVNGEAPDGPVENLFAVRRGPAGSRHFAFAGHLDVVPPGEGWTSAPFLPERRGELLYG
                     RGAVDMKGAIAAMVAACAAIPAQAGTVSLIITGDEEGAARHGTLALIEHMRETGEQPD
                     MCLVGEPTSVHRLGDMVKIGRRGSVNIWIEVEGKEGHVAYPHLADNPIPRLVAIMAEL
                     EALELDSGTDWFQPSNLEITDIAVGNPATNVIPARASARVSIRFNDLHTGNALARRIA
                     AIAHAHGGTARHVVSGEAFLTPPGDFSALLVEAIRAETGLEPDLSTTGGTSDARFLKA
                     VCPVVEFGLCNATMHKRDEAVAVEDLEVLARIYRQVAEAALAQSEPRSS"
     gene            complement(9033..9701)
                     /locus_tag="VN627_05820"
     CDS             complement(9033..9701)
                     /locus_tag="VN627_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXD02807.1"
                     /translation="MKLIIGNKNYSSWSLRGWLAARQSGLHFEEITIPLYGEGWDEQK
                     REIGEIAPSQGKVPVLWDGEAVVWDSLAIVDYLADKVGRDRFWPKPDDARAMARSMVA
                     EMHAGYQALRSECPMNIRRRVSRHQLTAQGRADVVRILTLWAEARARFGHGGPFLFGT
                     FCAADIFYAPVVSRFLTYVVPVPGFAEAYMQAVWEHDWLVQWVAAAEGEEWRLEQYDG
                     PSAA"
     gene            9834..10658
                     /locus_tag="VN627_05825"
     CDS             9834..10658
                     /locus_tag="VN627_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HXD02808.1"
                     /translation="MGTAALGIDHLTAIGMPPVDLIQLAEAAGLQSISLMTHRNYNPY
                     GFADWSLVDDARLRRETRAALAGSGVQLALGEACHIRPDATARDFEPVIAMFAELGCP
                     RMNVVSFEYEPVREAEQAHELAEMICAAGLTFNWEFSRRGRRMLADVAREVRAMRARG
                     LAAHILIDPMHFIRGGEQIANLAALEPELVSYFQLCDVPLVGEGEYMEEALYRRLPPG
                     DGELPLAEIVRALPEGAVISMEIPQRSRIEAGESFIDMVRDAAAKSRALLAEALGG"
     gene            complement(10647..>11321)
                     /locus_tag="VN627_05830"
     CDS             complement(10647..>11321)
                     /locus_tag="VN627_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02809.1"
                     /translation="LSYEVWYYALFGAAVFLGGSRRVIWLLAIGWLAGPLVLLLMPIW
                     LMGVALARLPALVRLGRPVGAVLLGVAAATFLLARTVDMPALAWLQAHSPLPLVNSQN
                     VVGNTLMGLAVALALAGVRPWLPVLAQPIALAAPALRWGAGMTFTAYLTHQPLICLAR
                     TWGLVAPPGASGAVLALVGILAVCALLASLCERRSPALRRSAQRWLARVYRREGQPPA
                     VGPLPA"
BASE COUNT         1771 a         3621 c         4206 g         1723 t
ORIGIN      
        1 ttaaccaata acgccgcccg gtgaaaggtg tcgtgggcgc gatgaatggt tggcctgcgc
       61 gacgtcagac gtggggccgg acctcttcca tgacccactg ggcgtgatcg agataggcct
      121 cgacgctcgc cactggcggc atcggcaccg agctcatggt gatgccgcat tcgcgcaggt
      181 ggcccagggc atcgaccagc tgctgggcac tggtcggatc cgggccgctc gcgccggacg
      241 cgccgtggcc ttcggtgagc agcaggttgg cgaggctgta ggtcacctcg agcggacgat
      301 cggtccaggc cggggcggac ttgatcgcct cgatccggtc gggcacctcg tcggggcggg
      361 tgcggaaggt gacgatccag cccgaaccga agcgggcgac gcgccgcagg gcggccgggc
      421 tatcgccccc gatccagatc ggcaggtggg gtttctgcac cggcttgggc tggaagccga
      481 tatcctcgaa ctgcacgtat ttgccgtggt aggtcggcct ctcctcagtc cacagggcga
      541 cgatcgccgc gagataatcg tccgcaatgg cgccgcgttc gcgccagggc acgccaaagt
      601 agggatattc atcctcgatc gagccgaggc cgaaggtcac cgcgatgcgg cctccgctca
      661 gccagtccgc cgtggctagg gccttggcgt ggacgatcgg attgctgaag ggcaggatcg
      721 ttatcccgga attgagcacg atccggctgg ttgccccggc gagcgccgcc tgggccgtgg
      781 tcgcgtggag ccagaaggca ccggtgtggt gatcagtcgg cggaacggcg tagtgttcgg
      841 gcaccgtgat catgtcgtag cccagctctt cggcgcggcg ggcgaggcgc agctggtcgg
      901 gcccggtcac ggtcagctcc cagggttggg tgacggcggc gatctccatc aggtgcggca
      961 tcgggaaggt gagtttcatc tgccggtcct gctcctgctt gcgttccggc gatggacgcc
     1021 ggaccccttt gcggacaggg tgggcgaggc cgagcgccgt tgcaagaggg caggtccgcg
     1081 cgcaagggtg tgccagtgca cggggccggg acccggtccg ggcctcttcc gttgacgcgg
     1141 ggaccgcggc gcaaccggag cgaaagagag catgggcctt tggccagacc agcagaccga
     1201 gcaccatcac gccgacggcc tgcccgtgcc gcgacgctac tttgcggcga tcacgatctg
     1261 gctggcgatc tcgatgtcgg tgctcgacgc cgccatcgcc aatattgccc tgccgacgat
     1321 tgccatggaa ctgcacgccg cgcctgccgc cgcgatctgg gtgatcaatg cctaccagct
     1381 ggcgaccacc atgctgctgc tgccgctcgc cgcgatgggc gaccggatcg gccatgcgcg
     1441 ggtgtatatc gggggcctgg cgctgttcgt tatcggctcg ctgctgtgcg cgctggcggg
     1501 gagcctgggc gcgctgatcg cggcgcgggt ggtccagggc atgggcgcgg ccggggtgat
     1561 gagcatgaac gccgcgctgg tgcgggccac ctacccctcc gacatgctcg ggcgggccat
     1621 gggctacaat gcgctggtgc tttccgtggc cgctgccgtg ggcccatcgc tggcctcggg
     1681 catcctggcc gtggcgacct ggccctggct gttcgcgatc aacgtgccgg tggggctcgc
     1741 ctcgctcgcg gtcggctggc actgcctgcc gcatgcgccg ggccatggcc gggcgcctga
     1801 cttcaccgcc gcggcgctga gcgcggccat gctggggctg gtggtctacg gcgcggagag
     1861 cttcgcccgc gaggggcgga cctcggggct ggtgctgctg gtgatcggcc tcgcctgcgg
     1921 ggccgggctg gtgatgtggg agcggggcaa ggcccatccg ctgtttccga ccgatctgct
     1981 cgcgcggccg gtctttgccc tctcgatcat ggcctcgacc acctccttcg cggcgcagac
     2041 cctggcctat gtctcgatcc cgttcatgct tcagcaggtg ctggggcgcg gcgtggtcga
     2101 gtccggcctg ctgatgacgc cgtggccgat cgcggcgggc acctgtgcgt tcatcgccgg
     2161 gcggctggca gaccgctggc cggcgggcct cctggggggc gcggggttgc tgatgttggc
     2221 gggcggtctg tttgctcttt cccggctggg cgcccacccc agcgacttcg accttgtctg
     2281 gcgcatggcc ctgtgcggcg cgggcttcgg cactttccag acgccgaaca accgcaccat
     2341 catcggctcg gccccgcgcg aacgggctgg cgcggccggt ggcatgctgg cgaccgcgcg
     2401 cctgcttggc cagacttcgg gcgcggtggc cgtggcggtg gggttccacc ggatgggact
     2461 gggctcctcc cccttcctgc tgtcctgcgg tgccgttgcc gccgtggtcg ccgccgggat
     2521 cagcctggtg cggctgcgcg tgcccgcgcc ggagggactg ccgggcgcgc gcgacgtggt
     2581 ggcggaatag ggcgccctgc cctcccccac ttccctcggc tcttccctcg gcgcgcgcgc
     2641 ttgcctaagc ggttggccat gagcctgccg cccgatcgcc tcgaccgctt tgcccgccac
     2701 atcgtgctcc ccgagatcgg cggggcggga caggtggcgc tggccagggc caacgtggtg
     2761 ctggtcggct gcggcgggat cggctcgccc gcgctgcaat accttgcggc agcgggcgtg
     2821 ggccagctga cactgatcga tgacgacgtg gtcgaagcga gcaacctcca gcgccagacg
     2881 atcttttcca ccggcgatat cgggcgcggc aaggccgctg ccgccgcgaa ctgggtgcgc
     2941 catttcgacc tcgcgctgga agtccacgcg cggcaggcgc ggatcgaggc gggcaatgcg
     3001 ggcgaactga tcgccggggc cgacgtggtg ctcgacgggt gcgacaactt cgccacgcgc
     3061 ctggcggttt cggacgcctg tgtgaaggcc ggcgtgccgc tgaccagcgc ggccctggga
     3121 cggttccagg gacaggtcgg caacttcgcc gggcacctgc cgggcgagcc ctgctaccgc
     3181 tgctgggtgg gcgatgcttt cgacgccgag gactgcgaca cttgcgccgc cgacggggtg
     3241 ctgggggcca tggcgggcat ggtcggctgc atgggggcca tggcggcgat ccgcgtgctg
     3301 ctggagggcc acgcggcgct gggcgacccg cagtggggca agctgcagct gctggacggg
     3361 ttggcgcctt cgctgcggac catccgggtg ccgaaggacg agacttgccg ggggtgtcgc
     3421 gcgcgataga tcctccccac gtgtggggag gtggcgcgcg cgcagcgcgt gacggagggg
     3481 gttctcgtcc gtgacggtgc gcctgcctga gagccccctc caccaccgcc tgcgggggcg
     3541 gtccccctcc ccacaagtgg agaggattta acagcccagc acttcctcta cccacccagg
     3601 caccagttcc gtcgccggcc cgaggcgggt ttcctcgaac caggcggagc ccctggagcg
     3661 ttccaggttg agttccagcg tggcggcgcc gagttcgcgg gccgagcgga cgaagccggc
     3721 ggcggggtag accgcgcccg aggtgccgat cgaaacgaac aggtcagcct cgcgcagggc
     3781 ggcatgaata cggtccatgc cgtagggcat ttcgccgaac cagacgacat cgggacgcag
     3841 cgcgctggcg ccgcagagcg ggcatggcgg gcgctcgatc agcggcccgg tccagcgggg
     3901 gcgccggtcg caggcggtgc accaggcggt gagatgttcg ccgtgcatgt ggtgcacgcg
     3961 gcgggcgccg gcgcgctcgt gaagatcgtc gacgttctgg gtcacgatga gaaggttgcc
     4021 cggccaggtg gaatcgagcc gggcgagcgc gtggtgcgcc gcgttgggct gcttggtctg
     4081 gatggcggcg cggcgcatgt cgtagaagcg caggacaagg tcgggatcgc gagcgaaggc
     4141 ttcgggggtg gcgacgtctt ccacccggtg ctgctcccac aggccgcctt ccgagcggaa
     4201 ggtgtcgatg ccgctctcgg ccgaaacgcc ggcgccggtg aggatcacga tgttgcggta
     4261 ttgagccatc gccgccatga agcacgccga caggaaggtg agcaatgtcc agaatcggaa
     4321 ttatcggcag cgagggacgc atgggcaagg cgctggcgga ggctgttgcc gctgcgggcg
     4381 aggtgctcgc gggcgggatc gacaagggcg gcgatccggt ggcgctggct gcggcatgcg
     4441 acgtgctcat cgacttttcg agccctcacg cgctggaggc caaccttgat gcggcggcgg
     4501 gagcgggcat accaattctg gtgggcacga ccggccttga ggagcggcat cactggctgg
     4561 tcgatgcagc ggcgcagaag accgccgtgc tccagacggg caatacctcg ctcggcgtca
     4621 acatgctcgc ccatctcgtg cgcgaggcag cggcgcggct gggtgaggac tgggacatcg
     4681 agattgtgga aacgcaccac cggatgaagg tggatgcgcc ctcgggcact gccctcctgc
     4741 tgggcgaggc cgcggccgag gggcgcgaga tccgcctggc ggacaatgcc gtgcgcgggc
     4801 gcgacgggat caccggcgcg cgggagagcg ggacgatcgg gtttgcttcg ctgcgcggcg
     4861 gcacggttgc gggcgatcac acggtctatt tcctggccga caacgagcgg atcgcgcttt
     4921 cgcaccttgc cgaaaaccgg ctgatcttcg cccggggcgc ggtgcgcgcg gcgcagtggc
     4981 tggtgggcaa ggcgcccgcg cgctaccgga tgcccgaggt actggggctt tgaagaagga
     5041 cgacatcttc gaattcttcc gccggctggc cgaggccaat ccctcgcccg agaccgagct
     5101 ggagttcggg aatgtctacc agctgctggt ggccgtgacg ctttcggccc aggcgaccga
     5161 cgttggcgtc aacaaggcga cgcgggcgct gtttcgcgcg gtgaagaccc cggcgcagat
     5221 ggtggcgctg ggcgacgagg ggctgaggga gcagatcaag accatcggcc tgttcaacac
     5281 caaggcgaag aatgtcatcg cgctctccga aatcctcgta cgcgaccatg ggggcgaggt
     5341 gccgaatgac cgggacgcct tgaccgcgct gccgggggtg ggccgcaaga cggccaatgt
     5401 cgtgctcaac tgcgcctttg gcgcggaaac gttcgcggtt gatacccata tcttccgcgt
     5461 gggtaatcgc accggtctcg ccaggggcaa gacgccgctg gcggttgagc tgcagctcga
     5521 gaagaaggtg ccgcagccgt ttcgccttgg cgcgcatcac tggctgatcc ttcacgggcg
     5581 ctatgtgtgc aaggcgcgca cgcccgagtg ttggcgctgc ccggtggtcg acctgtgcgg
     5641 ctacaaggcc aaggtgctgg agaaaggtgc gggcccggcg gcgaagaacg tgcgggaacg
     5701 ttccccgcgc aaatcggttc cggggacgcg actgccaaaa ggagatgcct gatgcgcctg
     5761 ggttcgttcc gtatcggcct tgccgtggcg gcctcctgcg cggcactggc cgggtgctat
     5821 ggctatgagc gcggtcccga tcgcgccggg ccggtggacc gaattcccgc cgccgccgtg
     5881 accgggccgg acgaggcctg cattccggtt caccagttca gccagaccgt ggtccgcgac
     5941 ggccggacga tcgacttcct gagcagcggg cggcgcggct ggcgcaatgt cctcccgagc
     6001 gactgcccgg gcctcgccag tgagcgggca ttcagcttca gcacctcgct ctcccagctg
     6061 tgcagcacgg acgttatccg ggtgctgcag aactatggcg gcgggctgca gccgggcgcc
     6121 acgtgcgggc tggggcggtt tacgccgatt gaactgcggc ggtaggccgt aacgcccaga
     6181 tcctccccgc ttgcggggac gggaccgccc gtgcagccgg tggtccgcag cgcggcgttc
     6241 gtctgaaagc cccctccgtc agcccttcgg gctgccacct ccccacgagt ggggaggatt
     6301 gcgggtcagg cttggggatc gaggttcagc cccttgcgcc catgctccgg cgtatcgtgc
     6361 ggggcttctg ggccttcccc atcggggtcg cgcaggggct gaagcatgcc ttcccacttg
     6421 gtgatcaccg ccgtcgccac ggcattgccg accacgttgg tggcggtgcg gcccaggtcg
     6481 aggaaggtgt cgacgccgag gatcagggca atgccctcca ccggcaggcc gaactgggtg
     6541 agcgtggcgg cgatgaccac gaggctggcg cgcggcacgg ccgcgatgcc cttagacgtg
     6601 accatcaggg tgagcagcat ggcgatctgc tggccggccg agaggtgaat gccgtaggcc
     6661 tgggcgatga acagggtggc gaagctcgta tacatcatcg agccgtcgag gttgaaactg
     6721 tagcccagcg gcaggacgaa gccggaaatg cggcggggga cgccgaaccg gtcaagctgc
     6781 tcgaacaggc gcggcagggc gctttccgag ctggccgtgg agaaggcgag gatcaccggc
     6841 tcgcggatgt agcgggccag cgtcacggca cgtttcttca ggaccagcgc ggccaggccg
     6901 aacaggatgg tccacaggat cagcagcccg aagtagaact cgctcatcag cgcggcatag
     6961 gtgaggacga tgccgaagcc gcggagcgcg atgaccgagg ccatggcccc gaacacggcg
     7021 agcggggcga agcgcatgac ataatccgtc gcccgcatca tcacctgggc gagcgcatcg
     7081 agcccttcga cgagtatcgc gccgcgttcg cgcagggccg agagggcgag gccgaagaac
     7141 agcgagaaga ccaggatctg gaggacattg ttcttggcca tggcgtcgac catcgaggtc
     7201 gggaaaactt cgaggacgaa gtggcggaag gtgaagtcgg acgtcgccag ctggccgata
     7261 tcgccaccgg ccttgaactg aaggccgacg ccgggctgga agaagttgac cagcagcagg
     7321 ccgacgagaa tcgagatgag gctcgcggtg atgaaccagg cgagcgccct gcccccgatc
     7381 cgcccgagcg ccgagctatc gcccatgccg gcgatgccgg tgaccagcgt ggcaaagacc
     7441 agcggggcga tgatcatctt gatgaggtgg aggaacacat cgggcaggag cttgagatag
     7501 tccgccacct gggcctggag cggatcgccc gcggggaacc acaggttgac cgccacgccg
     7561 gtaatgatgc cgagcacgag gccgataagg atgcggatgg tcagcttgcc ggtcattcag
     7621 gagccccttg cgatggggcg agactagcgg ggggcggggg cggcgcaata agcgttttgg
     7681 gattggggga acacgagcac gcggccagct atcagcgcaa cttcccctcc gtggtgagga
     7741 gggcacgaag ccgaatggct gagggaccgt cgcgaaccac aaagcggcgc gccaggccct
     7801 cgcgacggcg cttcagccgt tcggcttcag tgcctcctca ggacgaacgg ggttcggact
     7861 gcgccagcgc cgcctcggcc acctgccggt atatccgcgc caacacctcg aggtcctcca
     7921 ccgccacggc ctcgtcgcgc ttgtgcattg tggcgttgca caggccgaat tcgaccacgg
     7981 ggcagacggc cttgaggaag cgggcgtcgg aggtaccgcc ggtggtggaa agatcgggct
     8041 caagcccggt ttcggcgcgg atcgcctcga ccagcagggc cgagaaatcg ccgggcgggg
     8101 tgaggaaggc ctcgcccgag acgacgtggc gggccgtgcc gccatgggca tgggcgatcg
     8161 ccgcgatgcg cctggccagg gcattgccgg tgtgcaggtc gttgaagcgg atcgacaccc
     8221 gggccgaggc cctggcaggg atgacgttgg tggcgggatt gccgacggcg atgtcggtaa
     8281 tctcgagatt ggagggctgg aaccagtcgg tcccgctatc cagttcgagg gcctccagct
     8341 cggccatgat cgcgacgagg cgggggatcg gattgtcggc gaggtgcgga taggcgacgt
     8401 ggccttcctt gccttcgacc tcgatccaga tgttgaccga gccgcgccgg ccgatcttga
     8461 ccatgtcgcc gaggcgatgg accgaggtcg gctcgcccac gaggcacatg tcgggctgtt
     8521 cgccggtttc gcgcatgtgt tcgatgaggg cgagcgtgcc gtggcgggcg gcgccttcct
     8581 catcgccggt gatgatcagg ctgaccgttc cggcttgggc cggaatcgcg gcacaggcgg
     8641 cgaccatggc ggcgatcgcg cccttcatgt cgaccgcgcc gcgcccgtag agcagctcgc
     8701 ccctgcgctc gggcaggaac ggggcgctgg tccagccctc gcccgggggg acgacatcga
     8761 ggtggccggc aaaggcgaag tggcgggagc cggcggggcc gcggcggacg gcgaagaggt
     8821 tttcaaccgg gccatcgggc gcctccccgt tgaccatccg gcgcacttcg aagccgaggg
     8881 gcgcgagctg cccttcgagg caatcgaaca ccgcgcccgt cgccggagtg acgctgggac
     8941 aggcgatcag cgcctcggcg agatcggcga cggggggaag ctggctcatc gccctgccat
     9001 atggggaggg cgacggcgga acaagggcgg gctcatgccg ccgacggccc gtcgtactgc
     9061 tcgagccgcc attcctcgcc ttccgcggcg gcgacccact gaaccagcca gtcatgctcc
     9121 cacacggcct gcatataggc ttcggcgaac ccggggacgg gaaccacgta ggtaaggaag
     9181 cggctgacca cgggggcgta gaagatatcg gcggcgcaga aggtgccgaa caggaagggg
     9241 ccgccatggc cgaagcgggc gcgggcctcg gcccagaggg tgaggatgcg gaccacatcg
     9301 gcgcgcccct gcgcggtcag ctggtggcgc gacacccgcc ggcggatgtt catcgggcac
     9361 tcggaacgca aggcctggta gccggcgtgc atttccgcga ccatcgaacg cgccatggcg
     9421 cgggcgtcgt cgggcttggg ccagaaccgg tcgcgcccga ccttgtcggc aaggtagtcg
     9481 acaatggcga ggctgtccca gaccacggct tcaccgtccc acaggacggg gaccttgccc
     9541 tgcgacggcg cgatctcacc gatttcgcgc ttctgctcgt cccacccctc gccgtagagg
     9601 gggatggtga tttcctcgaa gtgcaggccc gactggcgcg cggcaagcca gccgcgcagc
     9661 gaccagctcg aatagttctt gttgccgatg atgagcttca cgaaggcctg cctggctggt
     9721 ctgacggtcc cgggtaaccg cccggggccg cgctgtcgag acagcgggtt tcacccgagg
     9781 ccggttcgtg ccaacaaagg cctcacaagc aacggaaaca agcggagaga accatgggca
     9841 cagcggcgct gggcattgac cacctgacgg cgatcggcat gcccccggtc gacctgatcc
     9901 agctggccga agcggccggg ctgcagagca tcagcctcat gacccaccgc aactacaatc
     9961 cctatggatt tgccgactgg tcgctggtgg acgacgcccg gctgcggcgc gagacccggg
    10021 cggcgctggc aggcagcggc gtgcagctgg cgctgggcga ggcctgccat atccgaccgg
    10081 atgcgacggc gcgcgatttc gagccggtaa tcgccatgtt cgccgagctc ggctgcccga
    10141 ggatgaacgt ggtttccttc gaatacgaac cggtgcgcga ggccgagcag gcccacgaat
    10201 tggccgagat gatctgcgcg gccggcctga ccttcaactg ggaattttcg cggcggggcc
    10261 ggcgaatgct ggcggacgta gcgcgcgagg tgcgggccat gcgcgcgcgg gggctggcgg
    10321 cgcatatcct gatcgatccc atgcacttca tccgcggcgg cgagcagatt gccaatctgg
    10381 ccgcgctgga gccggaactc gtcagctatt tccaattgtg cgacgtgccg ctggtgggcg
    10441 aaggcgaata catggaggag gcgctctacc gccgcctgcc gccgggcgac ggcgaattac
    10501 cgcttgccga gattgtccgt gccttgccgg agggcgcggt gatctcgatg gaaatcccgc
    10561 agcgctcgcg gatcgaggcg ggcgagagtt tcatcgacat ggtgcgggac gcggcggcca
    10621 aaagccgggc cctgctggcc gaggccttag gcgggtagcg gtccgacagc ggggggctgc
    10681 ccttcgcgcc gataaacccg ggcaagccac cgctgcgcag agcggcgcag ggccgggctg
    10741 cggcgttcac acaggctggc cagcagcgcg cagacggcca ggatgccgac cagcgcgagg
    10801 accgcgcccg atgccccagg cggcgcaacc agtccccagg tgcgggcgag gcagatcaac
    10861 ggctgatggg tgagataggc ggtgaaggtc atcccggcgc cccagcgcag ggccggagct
    10921 gcaagggcga tgggctgagc caacacaggc agccacggcc gcacaccggc cagggcaagc
    10981 gcaactgcaa ggcccatcag cgtgttgccg accacgttct gcgaattaac cagcggcagc
    11041 ggagaatggg cttgcagcca ggcgagcgcg ggcatgtcca ccgtccgcgc cagcaggaag
    11101 gtcgcggcgg ccacaccgag tagcacggcg ccaaccgggc ggcccagccg cacgagagcg
    11161 ggcaggcgcg cgagggccac gcccatcagc cagatcggca tgagcagcag gaccagcggg
    11221 ccagccagcc acccgatcgc gagcagccag atcacccggc gggaaccgcc gagaaacacg
    11281 gccgcgccga acagggcata gtaccagacc tcgtagctga g
//
LOCUS       DATNBV010000241         2548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1724909,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000241 DATNBV010000000
VERSION     DATNBV010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724909"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VN627_05835"
     CDS             <1..708
                     /locus_tag="VN627_05835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HXD02810.1"
                     /translation="TREAGIRVLVMTMDSPVAGNREYMLRNGFMPGMFNLRAALDALT
                     SPRWLLGTLLRYQLSGGLPQLSDMPEGHRIFFGKGNTGLCSPAADFTWDTVRQIRREW
                     GGMLVLKGLSAVEDAITAAEIGVDGIIVSNHGGRSLDGCVPSMGALPGIVDAVGSRAQ
                     VMVDGSFVCGVDVLKAIALGAKCVMVGRATAFGLAAGGEAGVSRALAILRDELHRGMA
                     LIGVRRLDELNRANLQF"
     gene            complement(734..1234)
                     /locus_tag="VN627_05840"
     CDS             complement(734..1234)
                     /locus_tag="VN627_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXD02811.1"
                     /translation="MARQTYTDAIALLKADHRKVEDLFEKFENARAGSKQKLAEEICN
                     ELKIHTIIEEEVFYPALKGKIEEDTWTEAYVEHDGAKVLVNDIAGGSSEDEFFDAKVH
                     VLSEEIKHHVREEERRQEGMFAQAREAGVDLIELKDRMLARKEELMAMAKNGGLPPAQ
                     VRVVQL"
     gene            complement(1512..1754)
                     /locus_tag="VN627_05845"
     CDS             complement(1512..1754)
                     /locus_tag="VN627_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02812.1"
                     /translation="MLTSSPRPQISPAPARDRDEVAARLLAPGDEVSIMGAGTYEIVH
                     LSHGRAWLSSLADGMQQLADATQLRLRRGGSAVARH"
     gene            complement(1820..2047)
                     /locus_tag="VN627_05850"
     CDS             complement(1820..2047)
                     /locus_tag="VN627_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02813.1"
                     /translation="MKTLSELLCPDVPLQTGCNATKSAGDHVMIGRNLGPVFQIVHIH
                     GDLAWVRPLANGQEGLVPLDRLRLVDIPLPN"
     gene            complement(2185..>2548)
                     /locus_tag="VN627_05855"
     CDS             complement(2185..>2548)
                     /locus_tag="VN627_05855"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HXD02814.1"
                     /translation="VHNFAVHALSRLRGGQPFPAAGCVVSPTYVPDLCHSVLDLLIDG
                     ESGLWHLSNGGAVSWAEFAQRIALACDLDASLVEQVSGADLGWTAPRPDRCALASERG
                     SPMPSLDAAIESFARAMA"
BASE COUNT          404 a          787 c          888 g          469 t
ORIGIN      
        1 acccgcgagg ccggtatccg ggttctggta atgacgatgg attcgccggt ggcgggcaac
       61 cgcgaataca tgctgcgcaa tggcttcatg ccgggcatgt tcaacctgcg cgcggcgctt
      121 gacgcattga cctcgccgcg ctggctgctt ggcaccctgc tgcgttacca gctatcgggc
      181 gggctgccac agctgtccga catgcccgag ggccaccgca tcttcttcgg caagggcaat
      241 accggcctct gttctccggc cgccgatttc acttgggaca cggtgcgcca gatccggcgc
      301 gagtggggtg ggatgctggt gctcaaggga ctgtcggcgg tggaggatgc gatcacagcg
      361 gccgagatcg gggtggacgg gatcatcgtc tccaatcacg gtggacgcag ccttgatggc
      421 tgcgttccct cgatgggggc gctgccggga atcgtcgacg cggtgggctc tcgcgcgcaa
      481 gtgatggtcg acggaagctt tgtctgcggg gtcgacgtgc tcaaggcgat cgcgctcggg
      541 gcgaagtgcg tgatggttgg ccgggccacg gcattcggtc tcgccgcagg gggcgaggcc
      601 ggggtgtccc gggccctcgc cattttgagg gacgagctgc accgcggaat ggccctgatc
      661 ggcgttcgcc ggctggatga gctgaaccgg gccaacctgc agttctgatc actgcaggcc
      721 ggcccgggcg cactcagagc tggacgacgc ggacctgcgc cgggggcagg ccgccgttct
      781 tggccatggc catcagctct tccttgcgcg ccagcatccg gtccttgagc tcgatcaggt
      841 cgaccccggc ctcgcgggcc tgggcgaaca tgccttcctg gcggcgctct tcctcgcgga
      901 cgtggtgctt gatctcttct gacagcacgt ggaccttcgc atcgaagaac tcgtcctcgc
      961 ttgagccgcc ggcaatgtcg ttgaccagaa ccttggcacc gtcgtgctcg acataggcct
     1021 cggtccaggt gtcttcttcg atcttgccct tgagcgcggg gtagaacacc tcttcctcga
     1081 tgatcgtgtg gatcttcagc tcgttgcaga tttcctctgc cagtttctgc ttggacccgg
     1141 cgcgggcgtt ctcgaacttc tcgaacaagt cctcgacctt gcggtggtcg gccttcaaca
     1201 gggcaatagc gtcggtgtag gtctggcggg ccatgaaatg ctccttgcgg gtcgcggggg
     1261 tgggactggc gcaatttcgc gcctcaccca ccaacaaggc gcgagagggc gcgccggttc
     1321 cggttttcag ccgcgaatag gccgagcgga gatcgcctgc gtttccccgc gttcgggacg
     1381 atcgcctatg agcgcccttc aaggggttag gtgcggatgg caggaagcgg ggccgaccga
     1441 cgccagctat cggaagccat cgatcagcga gtggtcaagg cctgcctgcc gtagcgcgcg
     1501 acagctcaac ctcagtggcg ggcaaccgcc gacccgccgc ggcgcagacg cagctgggtg
     1561 gcatcggcca attgctgcat cccatcggca aggctgctca gccaggcccg gccgtgcgac
     1621 aggtgcacga tctcgtaagt gcccgcgccc atgatgctta cctcatcacc cggggcgagc
     1681 aggcgcgccg caacctcgtc ccggtcacgg gccggggcgg gggagatctg cgggcggggg
     1741 gaggaggtga gcatgggtgc agatggaagc tgccggccat ggttcggttc ccgcccgaac
     1801 atgaattttt cggtaatttt cagttgggaa gtgggatatc caccagccgc agccggtcga
     1861 gcgggaccag gccctcctgc ccgttggcca gcggccgcac ccaggcgaga tcgccatgaa
     1921 tgtggacgat ctggaacact gggccgagat tgcggccgat catcacgtga tcgcccgcag
     1981 acttggtcgc gttgcagccg gtctggagcg gtacgtcggg gcagagtagt tcggagagcg
     2041 ttttcatgat cacacgtgta gcaggtggca acgccgcagc gttagacggt ttccttgcaa
     2101 agtgtaatcg gcgttctttg gtttcaacag gttagatgcc ctcggttagt cgcatttgcc
     2161 aatcgcggga caggggtcct gaacctaggc catggctctg gcgaagctct cgatggcggc
     2221 atcgagcgac ggcatgggcg aaccccgctc gctcgcgagg gcgcagcgat ccgggcgggg
     2281 cgccgtccag cccaggtccg cgccgctgac ttgttccacc agcgaggcgt cgaggtcgca
     2341 ggcgagcgcg atccgctggg caaattcggc ccagctcacc gcgccgccgt tggaaagatg
     2401 ccacaggccg gattcgccgt cgatcagcag gtcgagcacg ctgtggcaca ggtcgggcac
     2461 ataggtcggc gagaccacgc agccggccgc cgggaatggc tggccaccgc gcaggcgcga
     2521 gagcgcgtgg acggcgaagt tgtgcacg
//
LOCUS       DATNBV010000242         3174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_447258,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000242 DATNBV010000000
VERSION     DATNBV010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447258"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VN627_05860"
     CDS             complement(<1..141)
                     /locus_tag="VN627_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vgr related protein"
                     /protein_id="HXD02815.1"
                     /translation="MKGARPLTQGEIELARSMFGDAIDYGRVKMVRRKWWLFQPKGIV
                     MAP"
     gene            229..465
                     /locus_tag="VN627_05865"
     CDS             229..465
                     /locus_tag="VN627_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXD02816.1"
                     /translation="MSETADRVKKIVVEHLGVEAEKVTEEASFIDDLGADSLDIVELV
                     MAFEEEFGVEIPDDAAEKIATVKDAIDYIDSNKG"
     gene            570..1829
                     /gene="fabF"
                     /locus_tag="VN627_05870"
     CDS             570..1829
                     /gene="fabF"
                     /locus_tag="VN627_05870"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HXD02817.1"
                     /translation="MRRVVVTGLGLVTPLGGDVETTWANIISAKSGAGTITKFDASDQ
                     KCRIACEVKPADHEYGFDPNKRVDHKVQRQVDPFIIYGIDAAGQAIEDAGLTEMDEAT
                     RLRAGCSIGSGIGGLPGIESESLVLHEKGPGRVSPHFVHGRLINLISGQVSIKYGLMG
                     PNHAVVTACSTGAHSIGDAARMIRDDDADIMLAGGAEGTICPIGIAGFAQARALSTGF
                     NDEPTRASRPYDQARDGFVMGEGAGVVVLEEYEHAKARGAKIYAEVVGYGLSGDAYHV
                     TAPHPEGSGAFRSMQMALKKAGMTPDDIDYINAHGTSTPLGDELELGAVRRLFGDAIG
                     NVSMSSTKSAIGHLLGGAGAVESIFCILALRDQIVPPTLNLDNPSESCADVDLVPWKA
                     KQREVRAVLNNSFGFGGTNASLVMKKV"
     gene            2010..2969
                     /gene="mltG"
                     /locus_tag="VN627_05875"
     CDS             2010..2969
                     /gene="mltG"
                     /locus_tag="VN627_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXD02818.1"
                     /translation="MIRPKLLAMILALLALAWGLGGWFLPGPLAKDTEFVVKDGESFN
                     AVAEHLQYTGAISSPLTFKLRARLLGGGAPIKAGNFVLPRGASQRTLVDILQGGKVVR
                     RFVTVPEGMPSIMVAERLAAQPLLTGDTVTPPEGSILPDTYEVAAGESRQAVVLRMQA
                     AMRRTLQELWAKRSPATVAKTPEEALVLASIVEKETGKPSERAMVAGLYSNRLRGHMM
                     LQADPTIIYPITKGKPLGRRIRQSEIAAVNGYNTYSMIGLPEGPITNPGRASIEAVLN
                     PAKTDALYMVADGTGGHAFAATLEEHNRNVAKWFAIRRQRGEL"
BASE COUNT          572 a         1034 c         1046 g          522 t
ORIGIN      
        1 cggggccatg acgatcccct tgggctggaa tagccaccat ttgcggcgaa ccatcttgac
       61 ccgcccatag tcgatggcgt cgccgaacat cgaccgcgcc agctcaattt cgccttgggt
      121 aaggggacgg gcgcctttca tggccggaaa gctagtgccg caactcccgc ttgcccagtg
      181 tcgcggctgc cgctagggca acccaaaatc taatggagga aagtccacat gagcgagacc
      241 gccgaccggg tgaagaagat cgtcgtcgag catctcggcg tcgaagctga gaaggtcacc
      301 gaggaagcga gcttcatcga cgatctgggc gccgacagcc tcgacattgt cgagttggtg
      361 atggcgttcg aagaagaatt cggcgtcgag atcccggacg atgcggccga gaagatcgcc
      421 accgtcaagg acgcgatcga ctatatcgac agcaacaagg gctgatcggg accgatcggc
      481 tgccgcccgc cgaaaaggcg gcacgaacgg cttcccggac ctgtcgggag gccgtttgcg
      541 tttgaggacg gttggaaggg aatttctgca tgcgccgtgt cgtggtgaca ggtctggggc
      601 tggtgacgcc gctggggggc gacgtcgaaa ctacatgggc gaatatcatc tccgcaaaat
      661 ccggggccgg aacgatcacc aagttcgatg cgtcggacca gaagtgccgc attgcctgcg
      721 aggtgaagcc ggccgaccat gaatatggct tcgatcccaa caagcgggtc gaccataagg
      781 tgcagcggca ggtcgatccg ttcatcatct acgggatcga tgccgcgggc caggcgatcg
      841 aggatgccgg ccttaccgag atggacgagg cgacgcgcct gcgcgccggc tgctcgatcg
      901 gttcgggcat cggcggcttg ccggggatcg agagcgaatc gctcgttctt cacgaaaagg
      961 gcccgggccg cgtttcgccg cacttcgtcc atggccgcct tatcaacctg atctcgggcc
     1021 aggtctcgat caagtatggc ctgatggggc cgaaccacgc tgtggttacc gcctgttcga
     1081 ccggcgccca ctcgatcggc gatgccgcgc gcatgatccg cgacgacgat gccgacatca
     1141 tgctggcggg cggcgccgag ggtacgatct gcccgatcgg cattgccggc ttcgcccagg
     1201 cccgcgcgct ctctaccggc tttaacgatg agcccacccg ggccagccga ccgtacgacc
     1261 aggcccgcga cggcttcgtc atgggcgagg gtgccggcgt cgtcgtgctc gaagagtacg
     1321 agcacgccaa ggcccgcggc gcgaagatct atgccgaagt ggtcggctat ggtctttccg
     1381 gcgacgccta tcatgtcacg gccccgcacc cggaaggctc gggcgcgttc cgctcgatgc
     1441 agatggcgct gaagaaggcc ggaatgaccc cggacgacat cgattacatc aatgcccacg
     1501 gcaccagcac gcccctgggc gacgaactgg agctgggcgc ggtgcgccgc ctgttcggcg
     1561 atgcgatcgg caatgtctcg atgagctcga ccaagagcgc gatcgggcac ctgctgggcg
     1621 gcgcgggcgc ggtcgaatcg atcttctgca tcctggccct gcgtgatcag atcgttcccc
     1681 ccacgctcaa cctcgacaat ccaagcgaat cctgcgccga cgtcgatcta gtgccgtgga
     1741 aggccaagca gcgcgaagtg cgggctgtgc tgaacaattc gttcggcttc ggcggaacca
     1801 atgcctcgct ggtgatgaag aaggtctgat ggtgtcagct ggcgtcgtcc cggacttgat
     1861 ccgggaccgc tggcgaacct tcaggcgata cggcgccgat ctggcgaggt cacagcttcg
     1921 cagttgccag cggtcctggg tcaagcccgg gacgacgcag atcggcgggg catcttgatc
     1981 cgctgccgcg cttcgcccac accgcaccga tgatccgccc gaaactcctc gccatgatcc
     2041 tcgccctgct cgccctcgcc tgggggctgg gcggctggtt cctgccgggg ccgctggcga
     2101 aggacacgga gtttgtcgtc aaggatggcg agagcttcaa tgcggttgcc gagcatctgc
     2161 agtataccgg cgccatctcc tcaccgctga cgttcaagct gcgggcgcgc ctgctgggcg
     2221 gtggggcgcc gatcaaggcc ggcaatttcg tcctgcccag gggcgccagc cagcgcacgc
     2281 tggtcgacat ccttcagggc ggcaaagtgg tgcgccgctt cgtcaccgtg cccgagggca
     2341 tgccctcgat catggtagct gaacgcctcg ccgcccagcc cctgctgacc ggggacaccg
     2401 tgacgccgcc cgaaggctcg atcctgcccg atacctacga ggtcgccgcg ggcgagagcc
     2461 gccaggccgt ggtcctcagg atgcaggcgg ccatgcggcg gaccttgcag gagctgtggg
     2521 cgaagcgcag ccccgccacg gtggcgaaaa cgccggagga ggcgctggtc ctcgcctcca
     2581 ttgtcgagaa ggagaccggc aagcccagcg agcgggccat ggtcgccggg ctctattcca
     2641 accgcctgcg ggggcacatg atgctccagg ccgatccgac gatcatctac ccgatcacca
     2701 agggcaagcc cctcggtcgg cgcatccgcc agtccgagat cgccgcggtc aatggctaca
     2761 acacctattc gatgatcggc ctgcccgaag gcccgatcac caacccgggc cgcgcctcga
     2821 tcgaggccgt gctcaacccg gcgaagaccg acgcgctcta catggtggcc gatggcaccg
     2881 gcggccacgc ctttgccgcc acgctggaag agcacaaccg caacgtggcg aagtggttcg
     2941 ccattcgccg ccaaaggggc gagctctagg caggcagcga gcagcgtgcc ctggccagtt
     3001 cggccaaggc ggtgagggcg acgatcagcg ggaacaccaa ggtcccgatg tccttgcgcg
     3061 cgatcagtcc gaaaacggcg aacgaccagc tgaacttgcg ctgtcggttc ttcgggcctc
     3121 cccctgcctc tgccgtggaa cctgccgagt tccgccgggt tcttcaagtt ccgg
//
LOCUS       DATNBV010000243         8772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2066311,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000243 DATNBV010000000
VERSION     DATNBV010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066311"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VN627_05880"
     CDS             <1..458
                     /locus_tag="VN627_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXD02819.1"
                     /translation="RLYECADGRFVAVASAEKRTWEALCDGLGLPDLKPALGKWDDPD
                     AVTRRLAAVFVTRPAAEWADRLAPAGAAVTVVNRGEDLAGDPQVRARGAVVEIAGSAV
                     PASPVRLASVPEHTSPIPPATVGADTAAVLAAAGFSAEELAGLEQGGLI"
     gene            677..1264
                     /locus_tag="VN627_05885"
     CDS             677..1264
                     /locus_tag="VN627_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HXD02820.1"
                     /translation="MHPLFATRGVPGTHDVALGTSILDAFLRGTAARLPRFDKGRDER
                     LPEGDWPLLPRCCDLLVWEGWCLGAPPQTDEALAEPVNAREREEDPQGIWRGCVNAQL
                     AGPYRALFERMDLIAYLQAPSFETVFAWRRQQEEALRAARPDAPGLMDEAALHRFIAL
                     YERLTRHMIAVMPSRADLTLALDAERRCRSVNRRG"
     gene            complement(1261..2688)
                     /locus_tag="VN627_05890"
     CDS             complement(1261..2688)
                     /locus_tag="VN627_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HXD02821.1"
                     /translation="MAADEIADSWSILPFGLAGARRRRRDHPVACWVATALMVSLAAM
                     LRLAMGRALPAPFLPFYPAIMGAALLGGRPTGYAAVIASGGIAAYLFVHPLQNLAITY
                     SETLALALFLAVGCVVVEILSYLGRRADLLVERQVALTVRSADLARREAETAASLSEL
                     EALYDRAPVGLGFLDRDLRFVRVNQTLADMNGSSPAEHIGRCVWEIVPDLQASAEPSL
                     RRVLDTGETLTGIELIGETPARPGEKRHWIEMFYPVYGGKGEIHGVGICCSDVTDFRQ
                     AQERERLLTREVDHRAKNLLTVVQSVLHLTRSAETIEEYKAAVTGRIQALGRVHTILA
                     DNRWDGIPLADLARQELAPYGTAVAITGPGREIILAPSPGQALSMILHELATNSAKHG
                     ALSGDGRVTLDLSASEDRLTLRWAEHGGPAVEPQGRNGFGTSLIQTAVKRLLGGTLDF
                     RLEATGLICEIAFPLPPARERTEAR"
     gene            complement(2780..3718)
                     /locus_tag="VN627_05895"
     CDS             complement(2780..3718)
                     /locus_tag="VN627_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXD02822.1"
                     /translation="MGLHHHHDHGHGHGHGHHDHGPSSHGRAFAIGVVLNAAFVVIEA
                     AFGFLSGSMALVADAGHNLSDVLGLVIAWGASVLAARPPSARFTYGFKSSSILAALAN
                     AALLLMALGAITVESVRRLMAPEPVAGGTVVAVALVGIVINTATALLFAKGRHSDINL
                     RGAFLHMAGDAAVSAGVVIAGLVIERTGALWIDPVTSLVIVAVIAWGTWGLLKDAVKL
                     GLAGVPPGINEGAVRKFIMEQPGVTAVHDLHIWAMSTTETALTAHLVMPGGFPGDGFL
                     HELAHELEHDFGIGHATVQVETSANANCALESDRVV"
     gene            complement(3777..4949)
                     /locus_tag="VN627_05900"
     CDS             complement(3777..4949)
                     /locus_tag="VN627_05900"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HXD02823.1"
                     /translation="MIRTLSCRHLAPLALGVAALPAAALAADAPRTLDLSASYVADTV
                     GVAEGPQTGVRFVDLLRVDAGADLDRAVGWHGARAKFTVEAGTGAQPNVLAGTLEGID
                     NAETVLNRPRLFQAYLEQDLGPRARVQVGFIDLNSAFYATDSAALLLAPPFGIGSELG
                     ASGPNGPSIFPSTSPAVTLRLEPAKDVYLLAGGFNGEARVIGDPGGPAPLLRQGALLI
                     GEAGWTGNHGKLALGGWAYTRRQDDQRDTEPSGAPVRRTAWGVYGLVEQPVSPRLTVF
                     LRGGISDADTTPFAGSLQAGLRIGQVIAGRPDSELSAGFHAGFLAAKYRANLADAGEP
                     QRAVETGWELTYSDQLAPWLAVQPDVQYIRNAPRLTGSGDAVIVALRLTFTPPGQD"
     gene            complement(5130..5870)
                     /gene="mtgA"
                     /locus_tag="VN627_05905"
     CDS             complement(5130..5870)
                     /gene="mtgA"
                     /locus_tag="VN627_05905"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HXD02824.1"
                     /translation="MASLFTRSRARARTGTRGPARGGWGRRVATWAAKGILGFVALSL
                     LLVVLYKWVPVPITPTMIVDPNGFTKDWTSLSEIDRSMVAAVIAGEDSKFCSHNGFDT
                     AAMEKAWERNQRGGRIRGGSTISQQTAKNVFLWQNGGYFRKALEAWFTVLIEHVWGKR
                     RIMEVYLNVAETGIGTYGVEAGAQRYYGHSAARLSTVEAARIAAALPAPKKREVVGAS
                     GFTRRHGNVIAARSTVVRRSEYDGCVYD"
     gene            5931..6545
                     /locus_tag="VN627_05910"
     CDS             5931..6545
                     /locus_tag="VN627_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HXD02825.1"
                     /translation="MQLVLTIADPAIVCAVVPHGETAVIARLLTLEAGLVGAYVAGGR
                     GRQLRPVLIPGNLVAGEVRARAESQLPFARLELVRSRGPWLGEPLPAAAIVWATALAA
                     AALPEHQPFPALHSALTALLDAVCAAPSARGWLPALVAYEVLTLRELGYGAPASLPDA
                     QDIPANLALLDRLGDRLARYALAHRHSDVMAARAILRDRLGRIG"
     gene            6558..7601
                     /gene="leuB"
                     /locus_tag="VN627_05915"
     CDS             6558..7601
                     /gene="leuB"
                     /locus_tag="VN627_05915"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXD02826.1"
                     /translation="MKIAVFAGDGIGPEVTHEAVRVLQALDLPGLELTYGDVGAAAWR
                     KHGHPLPQVTLDVARAAEAILFGAVGDFSCDHLERHLRPEQAVLGLRKELGLFANLRP
                     AKVFAGLESHSALRPEIAGAIDLLIVRELNGDVYFGDKGIDDLGNGHRRGWDMMCYTD
                     DEVRRIAHVGFKAAQGRDGRLCSVDKANVLETSQVWRDTVIEVGKEYPDVELTHMYVD
                     NAAMQLVKNPGAFDVVVTGNLFGDILSDQASMCVGSIGLLASASMAVGTKGLYEPIHG
                     SAPDIAGQGNANPMAMILSAAMLLRHSFAREAEAARIEAAVAKALADGILGGDLGGTH
                     GTAAIGDAVLERL"
     gene            7628..8764
                     /locus_tag="VN627_05920"
     CDS             7628..8764
                     /locus_tag="VN627_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXD02827.1"
                     /translation="MNGNAIRLGIVLPTYNERGNIRTMVERLDAALAGIAWEVIVVDD
                     NSPDGTADEARSLAAEDRRVRVLQRIGRRGLSSAAIEGMCATSAPVVAVMDADHQHDP
                     ALLPGMLAAIEGGDYDLSYASRFAEGASTDAWGRPDRVKASGMANTIARKVTGVTLSD
                     PMSGYFMLRTDALRKDAHRLSGVGFKILLDILATVDQPLRIKEFPMHFAARAEGESKL
                     DQTVVFEFLVGLYDKWLGRIIPTRFALFGTVGAMGVFVQLGALWGLLHLVFGERFVYG
                     HWTGETTFNVANTLAAIVAMTFNFVLNNELTYSDKRLRGFAPLLRGWAKFAMTCSLGL
                     LTNIGSAAALKAMGFHDVVAVIIGIVLGSVWNFALSSKFVWGRY"
BASE COUNT         1336 a         3135 c         2917 g         1384 t
ORIGIN      
        1 ggcggctcta cgaatgcgcc gatggccgct tcgtcgcggt ggcctcggcg gaaaaacgta
       61 cctgggaagc cctgtgcgac gggcttggcc tgcccgacct caagcccgcg ctcggcaagt
      121 gggacgatcc agatgcggta accaggcggc tcgccgccgt gttcgtcacg cgcccggcgg
      181 cggagtgggc ggaccggctg gccccggccg gcgcggccgt cacggtggtg aaccggggcg
      241 aggatcttgc cggcgatccc caggtcaggg cgcgcggcgc ggtggtcgaa atcgccggga
      301 gcgcggtgcc cgccagcccc gtgcggcttg cctcggtgcc cgagcatacg tccccgattc
      361 ctcccgccac ggtcggcgcg gataccgcgg cagtgcttgc cgccgccggg ttttcggcgg
      421 aggaactggc cgggctcgaa caaggcggcc tgatctgagc gccgaggcgg ctccggtcga
      481 actcctcgct gcgacaatcg ccgccgcgcg ccgcgaagcg cctgaccggc cgctcatcgt
      541 cggcatcgcc ggcgcccagg gtagcggcaa gtcgaccctt gccgccaatc tggtcagccg
      601 gttccccggg gcagccacac tgtcgctcga cgatctctac ctgaccaagg ccgagcgcgc
      661 ccggctcgcg cgggaggtgc accccctgtt tgccacccgc ggcgtacccg gcacccacga
      721 cgtcgcgctc ggcacttcca ttctcgatgc cttcctccgc ggtactgcgg cccgcttgcc
      781 gcgcttcgac aaggggaggg atgagcgctt gccggaaggc gattggccgc tcctgccccg
      841 atgctgcgat ctcctggtgt gggaaggctg gtgtctgggc gctcctccgc agactgacga
      901 ggcccttgct gaaccggtca atgcgcgcga acgggaagaa gacccgcaag gcatctggcg
      961 cggctgcgtc aatgcgcagc tcgccgggcc ctaccgggcc ctgttcgaac ggatggacct
     1021 gatagcctac cttcaggcgc cttcgttcga gaccgtgttc gcctggcgcc gccagcagga
     1081 agaagccctg cgcgccgccc gccccgatgc gcccggactg atggacgagg cggcgctgca
     1141 ccggtttatc gccctttatg agcggctcac caggcatatg atcgccgtca tgccctcccg
     1201 cgccgatctc accctggcgc tcgatgccga acgccgctgc cgttcggtca accgccgcgg
     1261 ctagcgcgcc tcggtgcgct cccgggcggg tggcagcgga aatgcgatct cgcagatcag
     1321 ccccgttgcc tccagccgga aatccagcgt gccgccgagc agccgcttga ccgcggtctg
     1381 gatcagcgac gtgccgaagc cattgcgccc ctgcggttcg actgcgggcc cgccgtgttc
     1441 ggcccagcgc agggttagcc ggtcttcgct cgccgaaagg tcgagcgtca cccgcccgtc
     1501 cccggacagc gccccgtgct tggccgaatt ggtggccagt tcgtgcagga tcatgctcag
     1561 cgcctggccg ggggaggggg ccaggataat ttcccgcccc ggtccggtaa tcgccaccgc
     1621 cgtcccatag ggcgccagct cctgacgggc gagatcggcc aggggaattc cgtcccagcg
     1681 attgtcggca agaatggtgt ggacccggcc cagcgcctgg atgcggccgg tcacagccgc
     1741 cttgtattcc tcgatggttt cggccgagcg ggtcaggtgg agcaccgatt gcaccactgt
     1801 cagcaggttc ttggcccggt gatcgacctc gcgggtcagc aggcgctcgc gctcctgggc
     1861 ttggcgaaag tccgtcacat cgctgcagca gatgcccacg ccgtgaattt cgccctttcc
     1921 cccgtaaacc gggtagaaca tttcgatcca gtggcgcttc tcgcccggcc gggccggtgt
     1981 ctcgccgatc agttcgatgc cggtcagcgt ctcgccggtg tcgagcacgc ggcgcaggct
     2041 cggctcggcg gaggcctgaa ggtcggggac gatctcccag acgcagcggc cgatgtgttc
     2101 ggccgggctg gagccgttca tgtcggccag ggtctggttg acgcgcacga accgcagatc
     2161 gcggtcgaga aagccgagac cgaccggcgc gcggtcataa agagcctcca gttcgctcag
     2221 cgaggcggct gtctctgctt cgcgccgggc caggtcggcg gagcggacgg tcagcgctac
     2281 ctggcgttcg accagcaggt cggcacgccg gcccaggtag gaaaggattt caacgacgac
     2341 gcagccaacc gccaggaaga gcgccagcgc cagggtttcg ctgtaggtga tggccaagtt
     2401 ctgcagggga tggacgaaca gataggcggc aatcccgcca ctggcgatga cggccgcata
     2461 gccggtgggc cgccccccca acagtgcggc gcccatgatt gcgggataga atggcaggaa
     2521 cggcgcgggc agggcacggc ccatggcgag gcgcagcatc gccgccaggc tcaccataag
     2581 ggcggtggcg acccagcagg ctaccggatg atcgcgccgc cgccgtcgcg cgcccgccaa
     2641 gccgaacggg aggatcgacc aggaatcggc gatttcatcg gcagccatgc aaccccccgc
     2701 tgcaggaacg gcggaaacgc gttcctgccc aacactttgt cttgccgttg gcggctgcgc
     2761 aagggggctc ttgggacgtc tagacgaccc ggtcgctctc cagcgcgcag ttcgcattgg
     2821 cactggtttc cacctgcacc gtggcatggc cgatgccaaa gtcgtgctcc agctcgtggg
     2881 ccagttcgtg gaggaagccg tcgccgggga aaccgcccgg catgacgaga tgcgcggtca
     2941 gcgcggtctc ggtcgtgctc atagcccaga tgtgcaggtc gtgaaccgct gtgacgcccg
     3001 gctgctccat gatgaatttt cgcacggccc cctcgttgat ccccggcggc actccggcaa
     3061 ggcccagctt gaccgcatcc ttgagcagtc cccatgtgcc ccaggcgatc acggcgacga
     3121 tgacgaggct ggtcaccggg tcgatccaca gggcgccggt gcgttcgatc accagcccgg
     3181 cgatgaccac gccagcggaa acagctgcgt cgcccgccat gtgcaggaag gctccgcgca
     3241 ggttgatgtc gctgtggcgc cccttggcaa agagcagggc ggtcgccgtg ttgatgacga
     3301 tgccgacaag ggcaacggcc acgaccgtgc cacccgccac cggctcgggt gccatgagcc
     3361 ggcgcaccga ttccaccgtg atcgccccca gcgccatgag cagcagggcg gcattggcga
     3421 gggcggcaag aatggagctg gacttgaacc cataggtaaa ccgcgccgag ggcggccgcg
     3481 cggccagaac gctggcgccc caggcaatca ccaggcccag cacgtccgac aggttgtgcc
     3541 cggcatcggc caccagcgcc atcgaaccgg agaggaaacc gaatgcggct tcgatcacga
     3601 cgaaggcggc gttcagcact accccgatgg caaaggcccg gccgtggctc gaagggccat
     3661 ggtcgtgatg gccgtgcccg tgcccgtgcc cgtggtcatg atgatgatgc aggcccacgc
     3721 gcgactcctt cgcgcggccc tgtcgcacat gcggggcgtg gcggcaacgg gcgggttcag
     3781 tcctgcccgg gcggcgtaaa ggtcaggcgc aaagcgacaa ttaccgcatc gccgctgccg
     3841 gtcaggcggg gggcgttgcg gatgtactgc acgtccggct ggaccgccag ccagggcgcc
     3901 agctggtccg aataggtcag ctcccagccc gtctccaccg cccgctgcgg ctcgcccgcg
     3961 tcggccaggt tggcgcggta cttggcggcc aggaagcccg catggaaacc ggccgaaagc
     4021 tccgaatccg ggcgtccggc aatcacctgc ccgatccgca ggccggcctg caggctcccg
     4081 gcaaacggcg tggtatccgc atcggaaatc cccccgcgca ggaagacagt gaggcgtggg
     4141 ctgaccggtt gctcgaccag cccatagacg ccccaggccg tccgccgcac cggcgcgccc
     4201 gatggttccg tatcgcgctg gtcgtcctgc cgccgggtat aggcccagcc gccaagcgcc
     4261 agcttaccgt ggttgccggt ccaccccgcc tcaccgatca gcagcgcgcc ctggcgcagc
     4321 agcggggccg gtccgcccgg atcgccaatc acccgcgcct cgccgttgaa gcccccggcc
     4381 agcaggtaga catccttggc cggctccagc cgcagggtaa cggccggcga agtcgagggg
     4441 aagatcgaag gcccgttcgg cccgctcgcc cccagctcgc tgccgatccc gaagggcggg
     4501 gcaagcagca gggcggcaga gtcggtggca tagaacgcgc tgttgagatc gatgaacccc
     4561 acctgcaccc gcgcgcgcgg cccgaggtcc tgctcgagat aggcctggaa caggcgcggg
     4621 cggttgagca ccgtctcggc gttgtcgatc ccctccagcg ttccggcgag cacattgggc
     4681 tgtgcccccg tccccgcctc gacggtgaac ttcgcccgcg cgccatgcca gccgaccgcc
     4741 cggtcgaggt ctgccccggc gtcaacccgc agcaggtcga cgaagcgcac gccggtctgg
     4801 ggcccctcgg ccacgcccac ggtgtcggcc acatagctgg ccgacaagtc cagcgtgcgc
     4861 ggcgcatccg cggccagtgc ggcggcggga agcgcagcca cgccgagcgc gagcggggca
     4921 aggtggcggc aagacagggt gcggatcatc ggcggtcggt ccatcggcgg cggggctgtc
     4981 cggcgccgtc accgctgccg gggcaggggg gaacgggcga ggcggctggc gcggcgccgg
     5041 acagccctgt catagccgag gcgcctttcc ccccggtcag cgcaggctta gggattagtg
     5101 cgaggccacc ggcaacttcg gctgcaacct cagtcgtaga cacagccgtc gtattccgat
     5161 cggcgcacca ccgtgctgcg ggccgcgatc acattgccgt gccgccgggt gaaaccgctg
     5221 gcgccgacca cttcgcgctt cttgggggcg ggcagggccg ccgcgatccg ggccgcctcc
     5281 acggtggaga gccgcgccgc cgaatggccg tagtagcgct gcgccccggc ctccacgcca
     5341 taggtcccga tgcccgtctc ggcgacgttg aggtatacct ccatgatccg ccgcttgccc
     5401 cagacatgct cgatcagcac ggtgaaccag gcctccagcg ccttgcggaa atagccgccg
     5461 ttctgccaca ggaacacgtt cttcgcggtc tgctggctga tcgtcgagcc gccgcgaata
     5521 cgcccgcccc gctggttgcg ttcccacgcc ttctccatcg ccgcggtgtc gaagccgttg
     5581 tggctgcaga acttggagtc ctcgcccgcg atcaccgcgg cgaccatgct gcggtcgatc
     5641 tctgacaggc tggtccagtc cttggtgaag ccgttggggt ccacgatcat ggtcggcgta
     5701 atgggcaccg gcacccactt gtagagcacc acaaggagca ggcttagcgc cacgaagcca
     5761 aggatcccct tcgccgccca tgtcgccacg cgccggcccc agcccccgcg ggcgggcccc
     5821 cgcgttcctg tccgcgctct tgcccgcgat ctggtgaaca agcttgccat ggcgcgtccg
     5881 atagcgcggc ccgcttgccc ccgccaagcc cctgcgccac agttcggtgg atgcagctgg
     5941 ttctcaccat tgccgatccg gccatcgtct gcgccgtggt cccccatggc gagacagcgg
     6001 tgatcgcgcg cctgctcacg ctcgaggccg ggcttgtcgg ggcctatgtc gccggcggcc
     6061 gcgggcggca gctgcgcccc gtgctgatcc ccggcaatct cgtcgcgggc gaggtccgcg
     6121 cgcgcgccga gagccagctg cccttcgccc gcctcgaact cgtccgcagc cgcggcccct
     6181 ggctgggcga gccgctgccc gccgccgcta tcgtctgggc cacggcgctg gccgccgccg
     6241 ccctgccgga acaccagcct ttcccggcgc tccacagcgc gctcaccgcc ctgctcgatg
     6301 ccgtctgcgc ggccccgtcc gcgcgcggct ggctgcccgc cctcgtcgcc tacgaggtgc
     6361 tcaccctgcg cgaactcggc tacggcgccc ccgccagcct gcccgatgcg caggatatcc
     6421 ccgcaaacct cgccctgctc gatcggctgg gtgaccggtt ggcgcgctat gcgcttgccc
     6481 atcgccattc ggatgttatg gcggcgcgcg cgatcctcag ggatcggctc ggcaggatcg
     6541 gttagcagga cggattgatg aaaattgcgg tgttcgccgg tgacggcatc ggcccggaag
     6601 tcacgcacga agccgtgcgc gtgctgcagg cgcttgatct gccgggactg gaattgacct
     6661 atggcgatgt cggcgccgcc gcctggcgca agcacggcca cccgctgccc caggtcacgc
     6721 tcgatgtcgc ccgggcggcc gaagcgatcc tgttcggcgc cgtgggcgat ttctcctgcg
     6781 atcatcttga gcgtcacctg cgccccgaac aggccgtgct cggcctgcgc aaggaactcg
     6841 gcctctttgc caacctgcgt cccgccaagg tcttcgccgg gctcgaaagc cattcggccc
     6901 tgcgccccga aatcgccggc gcgatcgatc tcctgatcgt ccgcgagctc aacggcgacg
     6961 tctatttcgg cgacaagggc atcgacgatc tgggcaatgg ccaccgccgc ggctgggaca
     7021 tgatgtgcta caccgacgat gaagtgcgcc gcatcgccca cgtcggcttc aaggccgcgc
     7081 agggccgcga cggccgcctc tgctcggtgg acaaggccaa cgtgctggag acctcgcagg
     7141 tctggcgcga cactgtgatc gaggtcggca aggaatatcc cgatgtcgaa ctcacccaca
     7201 tgtatgtcga taatgccgcc atgcagctgg tgaagaatcc cggcgcgttc gacgtggtcg
     7261 tgaccggcaa cctgttcggc gatatcctgt ccgaccaggc ctcgatgtgc gtcggctcga
     7321 tcggcctgct cgcctcggcc tcgatggcag tgggcaccaa ggggctgtac gagccgattc
     7381 acgggtccgc gcccgatatc gccgggcagg gcaatgccaa cccgatggcc atgatcctgt
     7441 cggcggccat gctcctgcgc cactccttcg cccgcgaggc cgaggctgcg cggatcgagg
     7501 cggccgtagc gaaggccctg gccgatggca ttctcggggg cgatcttggt ggcacccatg
     7561 gcaccgccgc catcggcgat gccgtgcttg aacggctctg aacaaccatg cccggggggg
     7621 attggtgatg aacggtaatg ctatccggct cggcatcgtc ctgccgacct acaacgaacg
     7681 cggcaacatc cgcaccatgg tcgaacggct cgatgccgcg ctcgccggca tcgcctggga
     7741 agtcatcgtc gtcgacgata acagccctga cggcacggcc gacgaagctc gcagcctcgc
     7801 cgcggaagac cgccgcgtcc gcgtgctcca gcggatcggc cggcgcgggc tttcatcggc
     7861 ggcgatcgag ggcatgtgcg ccacctcggc gcccgtcgtc gcggtcatgg atgccgatca
     7921 ccagcacgat cctgccctgc ttcccggcat gctcgccgcc atcgagggcg gggactacga
     7981 cctgtcctac gccagccgct tcgccgaagg cgccagcacc gatgcctggg gccggcccga
     8041 tcgcgtcaag gcttccggca tggccaacac gatcgcccgc aaggttaccg gcgtcaccct
     8101 gtctgatccg atgagcggct atttcatgct gcgcaccgat gccctgcgca aggatgccca
     8161 ccgcctctcc ggcgtcggct tcaagatcct gctcgatatc cttgccacgg tcgatcagcc
     8221 gctgcgcatc aaggaattcc cgatgcactt cgccgcccgg gcggagggcg agtccaagct
     8281 cgaccagacg gtggtcttcg aattcctcgt cggcctctat gacaagtggc tgggccgcat
     8341 catccccacc cgcttcgccc tgttcggcac ggttggcgcg atgggcgtct tcgtccagct
     8401 cggcgcgctg tggggcctgc tccacctcgt cttcggcgaa cgcttcgtct atggccactg
     8461 gaccggagag acgaccttca acgtcgccaa taccctggcc gcgatcgtcg cgatgacctt
     8521 caacttcgtc ctcaacaacg aactgaccta ttccgacaag cgcctgcgcg gcttcgcccc
     8581 gctactgcgc ggctgggcca agttcgcgat gacctgctcg ctcggcctgc tcaccaacat
     8641 cggctcggcg gccgcgctca aggccatggg cttccacgac gtcgtcgccg tgatcatcgg
     8701 catcgtcctc ggatcggtct ggaatttcgc gctcagcagc aaattcgtct ggggccgcta
     8761 ctaaatcctc cc
//
LOCUS       DATNBV010000244         2535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2363293,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000244 DATNBV010000000
VERSION     DATNBV010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2363293"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..689)
                     /locus_tag="VN627_05925"
     CDS             complement(69..689)
                     /locus_tag="VN627_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXD02828.1"
                     /translation="MSGWWDRKVVPHIIRLGCGCQKLAELRQPIVSQASGKVLELGIG
                     AGANLRWYDTARVESLTGIEPSPELRAMATAARRPENPALEVIDAGAEALPFADASFD
                     SVVCTFTLCTVHDHHQAVREARRVLRPGGRLLFCEHGLAHDAGVQRWQRRIDPLWAPV
                     FGGCHITRAVRETIEQVFKIEQWQGGFQPGSPKLAGWMESGVATAI"
     gene            977..>2535
                     /gene="rpsA"
                     /locus_tag="VN627_05930"
     CDS             977..>2535
                     /gene="rpsA"
                     /locus_tag="VN627_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HXD02829.1"
                     /translation="MATSANPTRDDFAALLDETLGGAASGGFEGRVVKGTVTAIENDK
                     AVIDVGLKSEGRVALREFAMPGQPHGLSVGDEVEVYVDRVENSEGEAMLSRDRARREA
                     AWDKLESEFGEGKRVEGVIFGRVKGGFTVDLNGAVAFLPGSQVDIRPVRDVTPLMDIP
                     QPFQILKMDRRRGNFVVSRRAVLEETRAEQRSGLIQNLAEGQIIVGTVKNITDYGAFV
                     DLGGIDGLLHVTDMSYKRVNHPSEVINIGDEVKVQIVRINQDTQRISLGMKQLESDPW
                     DGVAAKYPVGAKLKGTVTNITEYGAFVELEAGIEGLVHVSEMSWTKKNVHPGKIVSTS
                     QEVDVLVLEVDSDKRRISLGLKQAIQNPWEAFAEKHPVGSTVEGEVKNATEFGLFIGL
                     DGDVDGMVHMSDIAWGISGEDALALHRKGEQVSAVVLDVDVEKERISLGMKQLERGGP
                     STAPATGGATAGDRLNKNAVVTVTVLEVRDGGLEVQAGDDGATGFIKRSDLGRDRDEQ
                     RPDRFQVGQKV"
BASE COUNT          460 a          877 c          822 g          376 t
ORIGIN      
        1 cacatcgcgt gggatgcgag caaggtgcgc tggccggtgg agcaggccga agcggccgaa
       61 tagaccgctc agatggcggt ggcgacaccc gattccatcc agccggccag cttcggactg
      121 cccggctgga agccgccctg ccattgctcg atcttgaaca cctgctcgat cgtctcgcgc
      181 acggcccgcg taatgtggca gccgccgaag accggggccc acagcgggtc gatccgtcgc
      241 tgccagcgct ggaccccggc atcgtgggcg aggccgtgct cacagaagag cagcctgccg
      301 ccgggccgca agacccgccg cgcctcgcgc acggcctggt gatgatcgtg gacagtgcac
      361 aaggtgaagg tgcacaccac ggagtcgaag ctggcatcgg caaagggcag ggcctcggcc
      421 ccggcgtcga tcacctcaag cgccggattt tccggccgcc gggccgctgt ggccatggcc
      481 cgcagctcgg gcgaaggctc tatcccggtc agagattcca cccgggccgt gtcgtaccag
      541 cgcaggttgg cgcccgcgcc gatgccgagt tccagaacct tgccgctcgc ctggctaaca
      601 atcggctggc gcagttcggc aagtttctgg cagccgcagc ccagccgaat gatgtgcggc
      661 accaccttgc gatcccacca gccactcatg ccaccctcgc gctctgtttg ggacagccta
      721 tgccgcaaac gctgccgcgt cgagggcact gtcgagcgca gggtccgccc ggccccattt
      781 cccttgcgtt tccgccgccc cgcgcctaag cgccgccggt cctgcaggcc gaatccggcc
      841 ggcgggatac tcgccacggc attcggcccg gcgggtggtt cggccctttt tggcccgttt
      901 ccggcaatgg tgccggggcg gaggaaagac cggcggacaa aaccgcctgg ccggaaacat
      961 cggtaaggaa accaacatgg caacttctgc caacccgacc cgcgacgatt tcgcagctct
     1021 ccttgacgaa acgctcggcg gcgccgccag cggcggcttc gaaggccgcg tggtcaaggg
     1081 caccgtcacc gccatcgaaa acgacaaggc cgtcatcgac gtcggcctga agagcgaggg
     1141 ccgcgtggcc ctgcgcgaat tcgccatgcc cggccagccg cacggtctgt cggtcggcga
     1201 tgaagtcgaa gtctatgtcg accgcgtcga gaattccgaa ggcgaagcca tgctgtcacg
     1261 cgaccgcgcg cgccgcgaag ccgcgtggga caagctggaa agcgaattcg gcgaaggcaa
     1321 gcgcgtcgag ggcgtgatct tcggccgcgt caagggcggc ttcacggtcg acctcaacgg
     1381 cgccgtcgcc ttcctccccg gctcgcaggt tgatatccgc cccgtgcgtg acgtcacccc
     1441 gctcatggac atcccccagc cgttccagat cctcaagatg gaccgccgcc gcggcaactt
     1501 cgtggtttcg cgccgcgccg tgctggaaga gacccgcgcc gagcagcgtt cgggcctgat
     1561 ccagaacctc gccgaaggcc agatcatcgt cggcaccgtc aagaacatca ccgactacgg
     1621 tgccttcgtc gacctcggcg ggatcgacgg cctgctccat gtcaccgaca tgagctacaa
     1681 gcgcgtcaac cacccgagcg aagtcatcaa tatcggtgac gaagtgaagg tgcagatcgt
     1741 ccgcatcaac caggacaccc agcgcatcag cctcggcatg aagcagctgg aatccgatcc
     1801 gtgggatggc gtggccgcca agtacccggt cggcgccaag ctcaagggca ccgtcaccaa
     1861 catcaccgaa tacggcgcct tcgtcgagct ggaagccggc atcgagggcc tggtccacgt
     1921 ttcggaaatg tcgtggacca agaagaacgt tcaccccggc aagatcgtct cgacctcgca
     1981 ggaagtcgac gtgctggtgc tcgaggtcga ttccgacaag cgccgcatca gcctgggcct
     2041 gaagcaggcg atccagaacc cgtgggaagc cttcgccgag aagcacccgg tcggttcgac
     2101 cgtggaaggc gaagtcaaga acgccaccga gttcggcctg ttcatcggcc tcgatggcga
     2161 cgtcgacggc atggtccaca tgtccgacat cgcctggggc atctcgggcg aggacgcgct
     2221 ggccctgcac cgcaagggcg agcaggtctc ggccgtcgtg ctcgacgtcg atgtcgagaa
     2281 ggagcgcatt tcgctcggca tgaagcagct cgagcggggc ggtccctcga ccgcgcccgc
     2341 cacgggcggc gccactgccg gcgatcgcct caacaagaac gcagtcgtca ccgtcaccgt
     2401 gctcgaggtc cgcgacggcg gtctcgaggt ccaggcgggc gacgacggcg cgaccggctt
     2461 catcaagcgc tcggacctcg gccgcgaccg cgacgagcag cgtccggacc gcttccaggt
     2521 cgggcagaag gtcga
//
LOCUS       DATNBV010000245         4399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2066768,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000245 DATNBV010000000
VERSION     DATNBV010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066768"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..2676)
                     /locus_tag="VN627_05935"
     CDS             complement(238..2676)
                     /locus_tag="VN627_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXD02830.1"
                     /translation="MALTTIRRAVRSPWSHCALLLATAVPVQAKEATHEEIVVTARRY
                     AEQLHDVPLAVEVVPRTAIRSGGIADLQSLSAHLPGLSFEAAWGGFNSFPVLRGQSQP
                     SIAGDNVGMFVDGVYQASRDALDVEPLDLDRIEVVEGPQSAHFGHSTFAGLISYVPAP
                     PTEAPLILGSADAGTDGLYGLRGTISGPLDHLFKGRLAASWRQANGTWTNSAAPGQQL
                     GNTRRLAIAGTLATRDGSGPLSVRLSARLGENRMSQPAFFALDFRSNNCGSRDPLAGA
                     WSYYCGEAPLPGAITLSPRLPDSRSHTGQVALHLALDLGAVQLQSDTSLYHAQSDSIR
                     DLDGSAEGDLYGVCVQGKSCSGVGSLTTPVARLQRVNIVLDRALSAREVAQEVRIRST
                     GKGFLGWEVGATLFWTRTRTALAFGGERGALAANERFAAPVLTDLQRVGAPAAINAAL
                     VNDPDLSQVPFSDSTERRRTIAAFIVADVRVRRNLGLRGELRSTWERLELDSRLANFL
                     PSFGASLGPRYFRDITPRISLDWRPDASWLAYVSYAKGSRSGGFNAFPQLRPDEQTYA
                     PETNWTAELGLKYAGSGVLRSAQIAAYDINWRNTQIQGLATTPGVSALIIRNTIGIHT
                     RGFEIAAKLAPTGWLGVDLAWSRADPRFKSGSEDPGSSGFCGLTTTNASSSFCTIRPS
                     TINPSQLVPDVSGNVLARAATSQWAIAAVVAPQTGALRGLRLRIEATHQGNVFERQID
                     GLYYGVRTLIGARLSVPVGRAEIEFWGTNLGDERYARVAAPRQPTFYTGLPRPVDLIL
                     ADRRRIGVTVRFHS"
     gene            complement(2678..>4399)
                     /locus_tag="VN627_05940"
     CDS             complement(2678..>4399)
                     /locus_tag="VN627_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02831.1"
                     /translation="ARDSALIGAASRLSPQDAGKELQTDYLIRGSVQRNGGTLRVISF
                     VVDTRSGTRRWSENFDWPVNRVYALQDRIADRIAASFRTQNDSDGVDASASAMTHNAD
                     AYLAYLKGKSLLGRFTIAETEAAAEQFERAVRYDPSFAPALVALFDSRMQAADLRRDD
                     LEAIRARYQPLLDRALQLAPNSGEALFAKAMWSNLPREGKAELFRHAAALEPNNSRGL
                     IAYAQFLGGGSNGGAYDGIGDGTVTRDGENGTERKRLLDHVLAIDPLNPRARFIAIMQ
                     RLGELTPSQVEEEQRKALDLDPDNYPLANRYAARLWMFHGETAEAIARMERVIATDPQ
                     NPWGPHIITAFYLDANDPVAAWAAAQSTPTSRESSRTILAQYRGDWRAAGQAALGPRG
                     FLFNRYQTWLWPETIRDYALQTKQYQLGAELIASRYGFDLRNPRTTSLPHTTAAPALG
                     HLLLAAGNRAAGERLLAQTVQWIDDHPRYGLSGLARVRAEALMLLGQRSQALSDLKAA
                     IDTAHDIRHWWYLVDRDPVWAAVHDDPRFREIVAHCRAAAQVQREKLDVLRRAGVVPQ
                     RPPLGRA"
BASE COUNT          791 a         1373 c         1456 g          779 t
ORIGIN      
        1 ccgccacagg cggagagcaa ggtgcacccg gccgccacca gcgatacagc cggaaagccc
       61 ctgcgcactg tctcgagttg gattttagcg atgttcagca tgggtccgct cctgacgatc
      121 tgcaactcgc ggcatctccg cggtggagga cgaacatacg acgaggcgcg ggcaagtgcg
      181 gtgaagcgac tgtgaagact ggccaaagct gcgccgaaat ttccgcgaga gcgggggtca
      241 gctgtggaag cgaaccgtta cgccgatgcg gcgccgatcg gccagaatga ggtcaactgg
      301 ccggggaagt cctgtataaa aagtcggctg gcgcggcgcg gccacgcggg cataccgttc
      361 gtccccgagg ttagtgcccc aaaattctat ctctgcccgg ccgactggca cgctcaagcg
      421 cgcgccgatt agggtccgga cgccgtagta gagcccgtcg atctggcgct cgaagacgtt
      481 gccctggtgg gtagcctcga tacgcagccg caagccgcgc agcgcacctg tctgcggcgc
      541 caccacggcg gcgatagccc attggctcgt tgcagcgcgg gcgagaacgt ttcccgacac
      601 atcggggaca agttggctcg ggttgatcgt actgggacgg atcgtgcaga acgacgatga
      661 tgcattggtg gtggtgagcc cacaaaaccc gctgctgccc ggatcttcgc tgccggactt
      721 gaaccgggga tcggcgcggc tccaggcgag atcgacaccc agccagccag tcggtgcgag
      781 tttggccgca atttcgaagc cgcgcgtatg tatgccgatg gtgttgcgga tgatcagcgc
      841 agaaacaccc ggtgtcgttg ccagcccctg gatttgcgta ttgcgccagt tgatgtcgta
      901 ggctgcgatc tgcgccgagc gcagcacccc ggatccggca tactttagcc ccagttcggc
      961 cgtccagttg gtctcgggcg cataggtctg ttcgtcgggg cgcaattgcg ggaacgcgtt
     1021 gaacccgccc gatcgcgagc ccttggcgta ggaaacgtag gctagccaac tggcatccgg
     1081 ccgccagtcg aggctgatgc ggggggtgat atcgcggaag taccgcggcc cgagactggc
     1141 cccgaagctc ggcaggaagt tggcgagacg gctgtccagt tccagccgct cccaggtcga
     1201 gcggagttcg ccgcgcagac cgagatttcg ccgaacgcga acatctgcga cgatgaacgc
     1261 ggcaatggtg cgccggcgtt cggtactatc gctgaacggt acctgcgaga ggtcagggtc
     1321 gttcaccaaa gccgcattga tcgccgcggg agcgccgacc cgctggaggt cggtcaggac
     1381 aggagcggcg aaccgttcgt tggccgccaa tgcaccgcgt tccccgccga aggccagcgc
     1441 cgtccgggtt cgggtccaga atagcgtggc gcccacttcc catcccagaa agcccttacc
     1501 ggtgcttctg atccgcactt cctgcgcgac ctcgcgtgcc gacagcgccc ggtcgaggac
     1561 gatgttgacg cgctgcagac gagcgacggg ggtggtcaga ctgccgacgc cactgcaact
     1621 cttaccctgc acgcacaccc cgtagagatc gccttcggca ctgccatcaa ggtcgcggat
     1681 cgagtcagac tgggcgtggt acaggctcgt gtccgactgc aattggactg ctcccagatc
     1741 gagcgcgaga tgcaaggcga cctgtcccgt gtgtgagcgg ctatctggca agcggggcga
     1801 cagggtaatt gcgccgggaa gcggcgcctc tccacaatag taactccagg ctcctgcgag
     1861 cgggtcgcga ctgccgcaat tgttggagcg gaagtcgagc gcgaaaaacg ccggctggct
     1921 catgcggttt tcgccaaggc gggccgatag ccgcacactg agtggcccgc ttccatctcg
     1981 ggtcgccagg gtgccagcaa tcgccagccg ccgcgtgttg ccaagctgtt ggccgggcgc
     2041 ggcggaattg gtccaggtgc cgttggcctg ccgccaactt gccgccagtc ggcccttgaa
     2101 taagtgatca agtggtccgc ttatggtccc gcgcagaccg tagagcccat cggtgcccgc
     2161 atcggcagag cccagtatga gcggcgcctc agtgggcggt gcggggacat agctgatcaa
     2221 tccggcgaag gtgctgtggc cgaaatgcgc gctttgtggc ccttccacca cttcgatccg
     2281 gtcgaggtcg agcggctcga catccagcgc atcgcggctc gcctggtaga ccccatcgac
     2341 gaacatgccg acattgtccc cggcgatgct cggctgactt tggccccgca ggaccgggaa
     2401 ggaattaaac ccgccccagg ccgcttcaaa tgacagaccg ggcagatgag cggaaagcga
     2461 ctgcagatcg gcgatgccgc ctgacctaat tgcggttcgt ggcacgactt cgaccgccag
     2521 agggacatcg tgcaactgtt cggcataccg gcgcgctgtg actacgattt cctcgtgagt
     2581 ggcctccttc gcctgcactg gcacagccgt agccagcagc agggcgcagt gggaccaggg
     2641 tgatctgacc gcgcggcgaa tagtggtgag ggccatgtca ggcccttcca agcggaggcc
     2701 tctggggcac aacgcccgcg cggcgcagga catcaagctt ctcgcgttgc acctgggccg
     2761 cagcgcggca atgcgcgacg atctcgcgga accgtggatc gtcgtggaca gctgcccaca
     2821 ccggatctcg atcgaccaga taccaccagt ggcggatgtc atgagccgta tcgatcgctg
     2881 ccttgaggtc gcttaatgcc tgcgaccgct gccccagcag cattaaggct tccgcccgca
     2941 cccgcgcgag gccggacaag ccatagcgcg gatgatcatc gatccactgc acggtctgag
     3001 ccagcaatcg ctctcccgcc gcgcggttac ccgctgccag caagaggtgg ccgagcgcgg
     3061 gtgccgccgt ggtatggggc aaactcgtgg tgcgggggtt gcgcaggtcg aagccgtagc
     3121 gagatgcgat cagctccgcg ccaagctggt attgcttggt ctgcaaggcg taatcgcgga
     3181 tagtctcggg ccatagccac gtttgatacc gattgaacag gaagccgcgc ggaccaagcg
     3241 ccgcctgtcc ggccgcgcgc caatccccgc gatactgggc aagaatcgtg cgggaggact
     3301 cgcgcgacgt cggggtcgac tgcgcggccg cccacgcggc cactggatcg ttggcatcga
     3361 gatagaaggc cgtgatgatg tgagggcccc aggggttctg cgggtctgtg gcgatgaccc
     3421 gttccattcg ggcgatggcc tcggcggttt cgccatgaaa catccacagg cgcgcggcat
     3481 agcggttggc caacgggtaa ttgtccggat cgagatccag ggccttgcgc tgctcctcct
     3541 cgacttggct tggcgtcaat tcgcccagcc tttgcatgat ggcgatgaac cttgcccgcg
     3601 gattgagagg gtcgatcgcc agcacgtggt cgagcagccg cttgcgttcg gtgccgttct
     3661 cgccgtcacg ggttacggtc ccgtcgccga tgccgtcgta ggcgccaccg tttgagcccc
     3721 cgccgaggaa ctgggcatag gcgatcagtc cgcggctatt gtttggctcg agcgcggcgg
     3781 cgtgccgaaa caattcggct tttccttcgc gtggcagatt actccacatc gccttggcaa
     3841 acagggcttc accggaattg ggggcgagtt gcaatgcgcg atccagcaac ggctggtagc
     3901 gtgcgcgaat ggcttccagg tcgtcgcggc gcaagtcagc ggcctgcatg cgcgagtcaa
     3961 acaacgcgac gagtgccggt gcgaatgacg ggtcataccg tacagcgcgc tcgaactgct
     4021 cggctgctgc ctcggtttcc gcgatggtga accggccgag gagcgacttg cctttgaggt
     4081 aggcgagata ggcatctgca ttatgcgtca tggccgatgc cgaagcatct acgccatcgc
     4141 tgtcgttctg ggtgcgaaac gacgccgcta tcctgtcggc aatccgatcc tgtagggcgt
     4201 agacgcgatt aacaggccag tcgaagttct ccgaccaccg ccgcgttcca gatcgcgtat
     4261 ccaccacgaa gctgatgacc cggagcgtgc cgccattccg ttgaacgctg cccctgatca
     4321 ggtagtccgt ctgcaattcc ttgccggcgt cttgcggaga aagccgtgaa gcagccccga
     4381 tcagggctga gtcgcgcgc
//
LOCUS       DATNBV010000246         2535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1728274,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000246 DATNBV010000000
VERSION     DATNBV010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728274"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VN627_05945"
     CDS             <1..410
                     /locus_tag="VN627_05945"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXD02832.1"
                     /translation="AIAAVYPDLAVETAAALDEVDFGEWQGRAFVELAGDPRWDEWNS
                     TRSTATTPGGETMAQAQKRAWRHVEQTAAALPGATIAMVSHCDVIRSLVAHVLGLGLD
                     RLLTFEIDPASLSRIEVGHGYARLLSLNEVVHG"
     gene            403..1188
                     /locus_tag="VN627_05950"
     CDS             403..1188
                     /locus_tag="VN627_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04290 family methyltransferase"
                     /protein_id="HXD02833.1"
                     /translation="MADSESLEEQVRALGPWFQNIRLGDGLTTAPDHFLGDYPAFKFA
                     GFADALPDDLAGRSVLDIGCNAGFYDIEMKRRGAGRVLGIDSDERYLAQARLVAQVLG
                     FPDIEYRNLSVYDVGALEEKFDLVVFMGVLYHLRHPLLALDLIREHVAGDMLLFQTMQ
                     QGSVDVVQVPEDHPFHVPGTMQPPSYFDNPGYPKMHFIERSFAHDWTNWWAPNAACSA
                     AMLRAAGFAIEAHPEPEVYLCRVSEVPFSQYGPAAVYPARGNP"
     gene            1250..2167
                     /locus_tag="VN627_05955"
     CDS             1250..2167
                     /locus_tag="VN627_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HXD02834.1"
                     /translation="MIEAAMIWNEPNNKSHWDPALDPDWSIYAELVRRAGTAIHAANP
                     AITRVLGGMSPIDAGWVERLRAMGALDMVDVVAVHGFPLDWNLWPIHDWPAKIAEIEA
                     VCPDKPVWVTEVGVGSFGAEEVQVFGVQKTAELLVGRAERVFWYSLYDLPQEWGATTR
                     HKEAEGSSYYRHFYMGLIRADGTPKPALADYARVADRMGLMQWFHFEDPRLDEAAAWM
                     KRLGTRHLRTGLSWADSFRPGALDWFDRQMEALADFDVTVTFCFTPEHMGPGAHHTSP
                     PHDCQAFADFCAAMIERYAPAKVAAEVAA"
     gene            complement(2172..>2535)
                     /locus_tag="VN627_05960"
     CDS             complement(2172..>2535)
                     /locus_tag="VN627_05960"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HXD02835.1"
                     /translation="AVHAVAFSLWVAVNERLVPGVTPFDPSLVMLATMASVEAIFLST
                     FVLIAQNRMAAAADRRADLDLHISLLAEAELTRLTDLTLRIAEKVGVDAPEPEIREVA
                     ELVDPGVVLDELERRESE"
BASE COUNT          415 a          799 c          893 g          428 t
ORIGIN      
        1 gggcgattgc cgcggtttat ccagacctgg ccgtggagac cgcggccgcg ctcgacgagg
       61 tggattttgg cgaatggcag gggcgtgcct ttgtcgagct ggcgggcgat ccgcgctggg
      121 acgaatggaa cagcacccgc agcacggcca ccacaccagg gggcgagacc atggcgcagg
      181 cccagaaacg tgcctggcgg catgttgagc agaccgccgc cgccctcccc ggcgcgacca
      241 tcgccatggt cagccattgc gatgtgatcc gcagcctggt ggcccacgtg ctcgggctgg
      301 ggctcgaccg gctgctgacc ttcgagatcg acccggcttc gctcagccgc atcgaggtgg
      361 gccatgggta cgcgcggctg ctttcgctta acgaggtggt ccatggctga cagcgaaagt
      421 cttgaggaac aggtccgcgc actggggccg tggttccaga acatccggct tggcgacgga
      481 ctgacgacgg cgcccgatca cttccttggc gactatccgg cgttcaagtt cgccggtttt
      541 gccgatgccc tgccggacga cctggcgggt cgctcggtgc tggatatcgg gtgcaacgcg
      601 ggcttctatg acatcgagat gaagcgccgc ggcgccgggc gcgtgcttgg catcgacagc
      661 gacgagcggt acctcgcgca ggcccggctg gtggcgcagg tgctgggctt tcccgacatc
      721 gaatatcgca acctgtcagt ctacgacgtg ggcgcgctgg aggagaagtt cgacctcgtg
      781 gtgttcatgg gggtgcttta tcatttgcgg cacccgctgt tggcactgga cctgatccga
      841 gagcatgtgg ccggcgacat gctgctgttc cagaccatgc agcaaggcag cgtggacgtg
      901 gtccaggtgc ccgaggacca tccgttccac gtgcccggaa cgatgcagcc gccgagttat
      961 ttcgacaatc ccggctaccc caagatgcac ttcatcgagc gcagttttgc gcacgactgg
     1021 accaactggt gggcgcccaa tgctgcctgc agcgcggcca tgctgcgcgc ggccggcttt
     1081 gcgatcgagg ctcatcccga gcccgaggtt tacctgtgcc gggtgagcga ggtgccgttc
     1141 agccagtatg gaccggcggc ggtttacccg gcgcggggaa atccatgatc tgccctcccg
     1201 cccgttcctc ggggtgggag gcgacaccga ttttgatgaa ggaccgccaa tgatcgaagc
     1261 cgcgatgatc tggaacgagc ccaacaacaa gagccactgg gacccggcgc tcgatcccga
     1321 ctggtcgatc tatgccgaac tggtgcgccg ggccgggacg gcgatccatg cggcgaaccc
     1381 ggcgatcacc cgcgtgctcg gcgggatgag cccgatcgat gcgggctggg tcgagcgact
     1441 gcgggcgatg ggcgcgctcg atatggtgga cgtggtggcg gtccatggtt tccccctcga
     1501 ctggaacctc tggccgatcc acgattggcc ggccaaaatc gccgagatcg aggcggtgtg
     1561 cccggacaaa ccggtgtggg tgaccgaggt tggggtcggc tcgttcggcg cggaggaggt
     1621 gcaggtcttc ggagtgcaga agaccgcaga actcctggtg ggccgggccg agcgcgtgtt
     1681 ctggtattcg ctttacgacc tgccgcagga atggggcgcg acgacccggc acaaggaagc
     1741 cgagggatcg agctactatc gccacttcta catgggcctg atccgcgccg acggcacgcc
     1801 caagccggcg ctggccgact atgcgcgggt ggccgaccgg atgggcctga tgcagtggtt
     1861 ccacttcgag gacccgcggc tcgacgaggc cgcggcctgg atgaagcggc tgggcacgcg
     1921 gcacctgcgc accgggcttt cctgggcgga ttcgttccga cccggggcgc tcgactggtt
     1981 cgaccggcag atggaggcgc tggcggactt cgatgtgacg gtgaccttct gcttcacgcc
     2041 cgagcacatg ggcccgggcg cccaccacac cagcccgccg cacgactgcc aggcctttgc
     2101 cgatttctgc gcggcaatga tcgagcgcta tgcgccggcg aaggtcgcgg ccgaggtggc
     2161 ggcgtgagcg actattcgct ctcccgccgt tccagctcgt ccagcaccac gccgggatcg
     2221 accagttcgg cgacctcgcg gatctccggc tcgggggcgt cgacaccaac cttttcggcg
     2281 atgcgcagcg tgagatcagt aaggcgcgtg agctcggctt ccgcgagcag gctgatgtgg
     2341 agatcgaggt cggcgcggcg atcggcggcg gcggccatgc gattctgggc gatgaggaca
     2401 aaggtggaga ggaagatcgc ttccaccgac gccatggtcg ccagcatcac cagcgacgga
     2461 tcgaacggcg tcacgcccgg cacgagcctc tcgttcacgg cgacccacag gctgaaggcc
     2521 accgcgtgga ccgca
//
LOCUS       DATNBV010000247         3657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3643679,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000247 DATNBV010000000
VERSION     DATNBV010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3643679"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1290
                     /locus_tag="VN627_05965"
     CDS             64..1290
                     /locus_tag="VN627_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HXD02836.1"
                     /translation="MKPLGPIPQGFRSKEGILQVDSQDVPGLVAEAGGTPLFVYSRAM
                     IADRVASLRAALPERVALHYAIKANPFSPLLHVMSELVDGFDVASGGELQKLVELDIA
                     ASRISFAGPGKRDAELTAAIAAGVTINLESEGEAERALRLAGEAGIRPRLAIRVNPDF
                     DLKGSGMKMGGGAKPFGVDAERVPVLARRIAEAGAEWRGFHIFAGSQAISADAIIETQ
                     AQTLTLAARLTQESGLSLPHLNLGGGFGIPYFPGDTPLDLAAVGAALAERMDDLPVEL
                     ADTDFCLELGRYLVGEAGVYLTTVIDRKVSHGEVFLVVDGGMHHQLAASGNFGTVVRR
                     NYPVAIATRFDAPVEEEASVVGCLCTPLDRLAEKGGFPRAEPGDIVAVFCAGAYGATA
                     SPMQFLGQGPAREMLV"
     gene            1510..2154
                     /locus_tag="VN627_05970"
     CDS             1510..2154
                     /locus_tag="VN627_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HXD02837.1"
                     /translation="MPFTRVSRLLAGCAVGTLALGGCAGTASGPQLPPASFVAMQEGP
                     GEDYVIGPLDDLTIFVWRNPELGASVQVRPDGRITTPLITDMPAVGKTPSMLAEDIKL
                     QLSQYIQEPLVSVIVNKFAGTFSQQVRVVGATTKPASIPYRANMTVLDAMIAVGGLSE
                     YAAGNKAKLIRFDKETGKQAEFALRLADLLRKGESKANVMLMPGDVIIIPESTF"
     gene            2171..>3657
                     /locus_tag="VN627_05975"
     CDS             2171..>3657
                     /locus_tag="VN627_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide chain length
                     determinant"
                     /protein_id="HXD02838.1"
                     /translation="MNGLIEQLRGALYTVWSRKWVALAAAWAICVVGWLAVAVIPNSY
                     ESRARIFVQLDDPLAEQIGIGVGDRRRDVDRVRQTLTSAVNLEKVIRNTSLGDGITTP
                     KQMEAMILALGKAIKVVSQQDNLFEITAVSSDGSLSDAESARLSHDIVQKMIDIFHEE
                     NLAGGRGEMTDTLDFMNQQLAARQKQLEEAEQRRLVFEAKYPELSAGGAGLLQRMEAA
                     RTQLRDTDADIAAAQSSLAAINGQLAGTPASMPGAGAGGAKGALAEATAQLSGMRARG
                     LTDSHPDVIAMKNQIAQLRAAAAAEGPGGGVPNPAYSSIQSIRAEKQANLQALQTRRA
                     AAQSDLARIDAQQTENPAVAAEAQRISRDYDVLKQQYDKLLQDREALRLRGQVETERS
                     AVKFQVIDPPTTPRSPSAPNRPLLLFAVLVVGIGGGIAAAFGLGHLRKTFQTSAHLEQ
                     ALGLPVLGAISLVKTDAFRAIEAKRLRYFMAGSGALGAVFLLLLV"
BASE COUNT          637 a         1210 c         1185 g          625 t
ORIGIN      
        1 ggccctgccg gtcggaccca acggcaagat tgaccgggcg ggtctcgcgg cggagttgtg
       61 cgaatgaagc ctcttggccc gataccgcaa ggattcagat ccaaagaggg cattcttcaa
      121 gtagattctc aagatgttcc gggtcttgtc gccgaggccg gcggcacacc gctgttcgtc
      181 tattcgagag caatgattgc ggatcgcgtg gccagcctcc gcgccgcctt gcccgaacgt
      241 gtcgccttac attatgccat aaaagcaaac ccattttcac cattacttca tgtaatgtct
      301 gaactggtag acgggttcga cgtggcctcg ggcggcgaac ttcagaaatt ggttgaactt
      361 gatatcgccg cctctcggat cagcttcgcc ggccctggca agcgcgatgc cgagctgacg
      421 gcagccatcg ctgccggggt gaccatcaac ctcgagagcg agggcgaggc ggagcgcgcc
      481 ttgcgccttg cgggcgaggc cgggatcaga ccccggctcg ccatcagggt gaatccggat
      541 tttgacctca agggttcggg catgaagatg ggcggcgggg ccaagccgtt cggggtcgat
      601 gccgagcggg tgccggttct ggcccggcgc atcgccgaag ccggagccga gtggcgcggc
      661 tttcacatat ttgccggcag ccaagcgata tcagccgacg ctatcattga aactcaagcc
      721 cagaccctca ccctggcggc gcgactgacg caggaatccg gcctgtcgct gccgcacctg
      781 aacctcggcg gcggcttcgg cattccctat ttcccaggcg acacgcccct cgatctcgca
      841 gccgtcggcg cagccttggc ggagcgcatg gacgatcttc ccgtagaact ggcggacacc
      901 gatttttgcc ttgaactcgg tcgctatctg gttggcgaag cgggggttta cctcaccacg
      961 gtgatcgacc ggaaagtcag ccatggcgag gtgttcctcg tcgttgatgg cggcatgcac
     1021 caccagctcg ccgcctcggg caatttcgga acggtcgtgc ggcgcaatta tcccgtggcg
     1081 atcgccaccc ggttcgatgc gccggtggag gaggaggcca gcgttgtcgg ctgtctgtgc
     1141 acgccgctcg acagactggc cgaaaagggc ggttttccgc gggctgagcc gggcgatatc
     1201 gtcgcggtgt tctgcgcggg cgcctatggt gcaaccgcca gcccgatgca attcctcggc
     1261 caaggcccgg cgcgcgaaat gctggtctga ggagatcccg cgcattgggt aaagcaagtt
     1321 aatccgcgat ctacggactg tccctttgcg ggaccctgcc caaattcggc cgtcgcaata
     1381 cttgatcact aaccgcttat tcaccctttt cgacgatagc ggaggggaga tttggcgcgg
     1441 gctgcgccgt ggggatgggc cggttggccc ggcccgcggt gggccgggct gcaagcgagg
     1501 actgtccaga tgccctttac ccgcgtttcc aggctgcttg ccggctgtgc cgtgggtacc
     1561 ctcgcgcttg gcggctgcgc cggcactgca agcggtccgc aactgccgcc ggcctcgttc
     1621 gtcgccatgc aggaagggcc cggcgaagac tatgtcatcg ggccgctcga cgatctgacg
     1681 atcttcgtct ggcgcaaccc tgaactgggg gcttcggtcc aggtccggcc cgacggccgg
     1741 atcaccacgc cgctgatcac cgacatgccc gccgtgggca agacgccttc gatgctggcc
     1801 gaggacatca agctccagct gtcgcaatat atccaggagc cgctggtctc ggtcatcgtc
     1861 aacaaattcg cgggcacgtt cagccagcag gtccgcgtcg tcggcgccac gaccaagccg
     1921 gcctcgatcc cttatcgggc gaacatgacc gtgctcgatg ccatgatcgc ggtgggtggg
     1981 ctgtccgaat atgcggccgg caacaaggcc aagctgatcc gcttcgacaa ggagaccggc
     2041 aagcaggccg agttcgcgct gcgcctcgcc gacctgctgc gcaagggcga gagcaaggcc
     2101 aatgtcatgc tgatgccggg cgatgtgatc atcatccccg agagcacctt ctgagggcgg
     2161 cccgggctca atgaacggtc tcatcgaaca actgcgcggc gcgctttaca cggtctggtc
     2221 ccgcaagtgg gtggccctgg ccgcggcctg ggcgatctgc gtggtgggct ggctggctgt
     2281 tgcggtcatt cccaattcct acgaatcgcg cgcccgcatc ttcgtccagc tcgatgatcc
     2341 gctggccgag cagatcggca tcggcgtggg cgatcgccgc cgcgatgtcg accgcgtgcg
     2401 ccagacgctg accagcgccg tcaacctcga gaaggtgatc cgcaacacat cgctgggcga
     2461 cggcatcacc acgcccaagc agatggaggc gatgatcctc gcgctgggta aggcaatcaa
     2521 ggttgtcagc cagcaggaca acctgttcga gattaccgcc gtctcatcag acggcagcct
     2581 gtccgacgcg gagagcgcgc ggctcagcca cgacatcgtc cagaagatga tcgacatctt
     2641 ccacgaggag aacctcgccg gcggccgggg cgagatgacc gatacgctcg acttcatgaa
     2701 ccagcagctc gccgcgcggc agaagcagct ggaagaggcc gaacagcgcc ggctcgtctt
     2761 cgaggccaag tatcctgagc tttcggcagg cggggctggt ctcctccagc gcatggaagc
     2821 tgcccgcacg caattgcgcg ataccgatgc cgatatcgcc gcggcgcaga gctcgctcgc
     2881 cgcgataaac ggacagctgg ccgggacgcc cgcatcgatg ccgggagccg gagcgggcgg
     2941 ggcgaaaggt gccctggccg aggccactgc gcaactttcc ggcatgcgcg cccgcggcct
     3001 caccgacagc caccccgatg tcattgcgat gaagaaccag attgcccagc tgcgcgcggc
     3061 cgccgccgcc gaggggccgg gcggaggcgt gccaaacccc gcctattcgt cgatccagtc
     3121 gatccgggcg gaaaagcagg ccaaccttca ggccctccag acgcgccgcg ccgccgccca
     3181 gagcgatctg gcccgcatcg acgcccagca gaccgagaac ccggccgtcg ccgccgaggc
     3241 ccagcgcatc agccgcgact acgacgtgct caagcagcag tacgacaagc tcctgcagga
     3301 ccgcgaggcc ctgcgcctgc gcggccaggt cgagaccgag cgcagcgcgg tcaagttcca
     3361 ggtcatcgat ccgccgacca cgccccgcag tccctcggcg cccaaccgcc cgctgctgtt
     3421 gttcgccgtg ctggttgtcg ggatcggtgg cggaatcgcg gctgccttcg ggctcggcca
     3481 tttgcgcaag accttccaga ccagcgccca cctcgaacag gcactcggct tgccggtgct
     3541 cggcgccatc tcactggtca agaccgacgc cttccgcgcc atcgaggcca agcggctcag
     3601 gtatttcatg gcgggcagcg gcgcgcttgg cgcagtgttc ctgctcctgc tggtgag
//
LOCUS       DATNBV010000248         2098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2067623,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000248 DATNBV010000000
VERSION     DATNBV010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067623"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VN627_05980"
     CDS             <1..718
                     /locus_tag="VN627_05980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD02839.1"
                     /translation="AGSHATLSGKVSRVPWGRHADAVVLLARDPAGAWTTVVVRGAAV
                     AEKGANFANEPRDTFVLDGLRVPIADVGKPGESLDPAGLRFIGARFRAAQMAGALSRV
                     LALSVQYAKERIQFGKPIGQFQAVQQQLAVLASQSAAANAAFDALTSALDSGPADFET
                     AAAKARIGEAGHVGAGIGHAVHAAMGFTQEHSLHRFTRRIWSWRDEFGSEIDWAEWLG
                     AEVLAAGGDGLWPLILGERP"
     gene            852..1799
                     /locus_tag="VN627_05985"
     CDS             852..1799
                     /locus_tag="VN627_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXD02840.1"
                     /translation="MSEFEHLLGGLAGLTAAFFWAVSTTIWGMVGRSIPAHSLVVIRV
                     TLAVGLLMLSNRVMTGSFLPGEVAAETLVLLSLSGFLAMVVGDYLFFKAVTRIGPRLL
                     MIVFAATPIVTAGIAWMVMGETVSPRGMVGIAVIVGGIVWVVSEPHGSAAWNQDDPRQ
                     FRIGIVLATMSLLATGSAYALTRAAVSGGERLFTEGPALPPVASADAALFRLAAAGVL
                     AWVALPFSPARRPTFATVANPRLMRFVVPGTVAGLFLGSWFSVMALSRVPGGIASALL
                     SCSPIFMIPLTRLFFGERHSPRALIGTLVTIAGVFVLLV"
     gene            complement(1813..>2098)
                     /locus_tag="VN627_05990"
     CDS             complement(1813..>2098)
                     /locus_tag="VN627_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXD02841.1"
                     /translation="SAQRLRPDLLLLDLGLPDLSGYAVCGTVRAALGSNVNIVAMTGW
                     GQEEDRQRTANAGFDHHLVKPVDASDVERVLREWEDKRSHGTGQGGTGRG"
BASE COUNT          272 a          708 c          723 g          395 t
ORIGIN      
        1 cgccggctcc cacgccacac tgtccggcaa ggtatcgcgc gtgccttggg ggcgtcatgc
       61 cgacgccgtg gtgttgctcg cccgggatcc ggcgggcgcg tggaccaccg tggtggtccg
      121 cggcgctgcc gttgccgaga agggcgcgaa cttcgccaac gagccgcgcg acaccttcgt
      181 gctggacggg ctgcgcgtgc ccatcgccga cgtgggcaag cccggtgaaa gcctggaccc
      241 tgctggcctg cgcttcatcg gggcccggtt ccgcgcggcg cagatggcag gggccctctc
      301 gcgggtgctg gcgctctcgg tgcagtacgc caaggagcgc atccagttcg gcaagcccat
      361 cggccagttc caggccgtgc aacagcagct ggcggtgctg gcgtcgcaat cggcggcggc
      421 caacgccgcc ttcgatgcgc tcacctccgc gctcgatagc gggccggcgg atttcgaaac
      481 cgcggccgcc aaggcgcgca tcggggaggc cgggcacgtg ggcgccggca tcggccacgc
      541 cgttcacgcg gccatgggct tcacccagga gcattcgctg catcggttta cgcggcgcat
      601 ctggtcgtgg cgcgatgaat tcggcagcga gatcgactgg gcggaatggc tgggggccga
      661 agtcctggcg gcgggtggag acgggttatg gccgctgatt ctcggggaac gtccatgagc
      721 cggcaggcgc cggcgccgcg gccccacctc cccagcgtgc gtttgagggc ttcagggaca
      781 gtcagaccat cgccgacgcg atgacgcgct tggtggactg acgcccgaag ctcgatctgc
      841 ctggtctcat catgtccgag ttcgaacacc tgctgggcgg cctggccgga ctcaccgcgg
      901 cgttcttctg ggccgtgtcg acgacgatct ggggcatggt cggtcgcagc attccggcac
      961 attcgctggt cgtcatccgg gtgacgctgg ccgtggggct gctgatgctg agcaaccgcg
     1021 tcatgaccgg ctctttcctg cccggagaag tagccgccga gacgctggtg ctgctttcgc
     1081 tcagcggttt tctggccatg gtcgtgggcg attacctttt tttcaaggcg gtgacgcgaa
     1141 tcggcccacg cctgctcatg attgtcttcg ccgccacgcc catcgtgacg gcgggcattg
     1201 cctggatggt catgggtgaa acggtgtcgc cgcgagggat ggtgggcatt gcggtgatcg
     1261 tcggcggcat cgtctgggtg gtttcggaac cgcatggatc ggcagcctgg aaccaggacg
     1321 atccacggca attccgcatc ggcattgtcc tcgccacgat gtccctgctg gccacgggct
     1381 cggcctacgc gctcacgcgg gcggccgtga gcggaggcga gcgcctgttc accgaggggc
     1441 ccgcgctgcc gcccgtggcc tcggccgacg ccgcgctgtt ccggcttgcc gcggccggcg
     1501 tgctggcctg ggtcgccctg ccgttctcgc ccgcgcggcg gccgacgttt gcgacagtgg
     1561 ccaaccccag gctcatgcgc ttcgtggtcc ccggaaccgt cgccgggctt ttcctcggtt
     1621 cgtggttttc cgtgatggcc ctgtcgcgcg tccccggcgg catcgcctcc gcgctgctga
     1681 gctgcagccc gattttcatg atcccgctga cccggctttt tttcggagag cgccactccc
     1741 cgcgggcgct gattggaacg ctggtgacca tcgccggcgt gttcgtgctg ctggtctaag
     1801 gcgctgcccg cctcacccgc ggccggttcc gccttgtcca gtgccgtggc tccgtttgtc
     1861 ttcccattcc cgcaataccc gttcaacgtc cgacgcgtcg acgggcttga ccaaatgatg
     1921 atcgaagccg gcgttggcgg tacgctggcg gtcctcttcc tggccccatc ctgtcatcgc
     1981 cacaatgttg acattgctcc ccagggcggc tcgaacggtc ccacacaccg cataaccgct
     2041 cagatcggga aggcctagat cgagcagcag aaggtctggc cttagccgtt gggcggac
//
LOCUS       DATNBV010000249         2489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1423332,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000249 DATNBV010000000
VERSION     DATNBV010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423332"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VN627_05995"
     CDS             <1..144
                     /locus_tag="VN627_05995"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994002.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXD02842.1"
                     /translation="HPIGASGARILVTLVAALRARGLRRGLASICIGGGEATAVAVEC
                     AEA"
     gene            complement(181..1404)
                     /locus_tag="VN627_06000"
     CDS             complement(181..1404)
                     /locus_tag="VN627_06000"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXD02843.1"
                     /translation="MAGPLTGIRVLDMTRVIAGPLAGQTLGDLGADVIKVERKGEGDE
                     SRRVGPPWMPEHEGIETPDSTYYQAVNRNKRSIAVDFATEAGADLVRRLADRCDVLLE
                     NYRPGTLDRYGLGANQLRQRNPGLIYCSLTGFGQTGPYSSRSGYDYLVQGMGGLMSVT
                     GLADGEPGAGPIRVGIPVVDIFGGMNATIGILAALRHRDATGEGQNIDIALYDSQLAA
                     MLNAASSWLNAGVHLGRTGNDHPSAVPYGIYEASDGYVIIATFNDREFVRLAEVLGRP
                     DWKDDPRYKSMGARVANRAAIKAEVAAIVIRQPRAHWVKLLNEAKISTGPINEMSDVA
                     ADPQTLARDMVVTMEHPVIGPIRAMGSPLKLSASPPEYRMAPPLLGENTNDILRSVLG
                     ADEAEIAALAEAGAI"
     gene            complement(1404..1811)
                     /locus_tag="VN627_06005"
     CDS             complement(1404..1811)
                     /locus_tag="VN627_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HXD02844.1"
                     /translation="MTLDPADMTIPRPDREFTEFLRQGRFMIQRSRSSGRHVFYPRVA
                     EPGTGARDLEWVEASGLGTVYATTTIYPRAPEAPYNVVLVDLDEGPRMMSRVVSAAPD
                     APIGLRVRARIDQSGDTPLVLFEPVAGEQGDLG"
     gene            complement(1808..>2489)
                     /locus_tag="VN627_06010"
     CDS             complement(1808..>2489)
                     /locus_tag="VN627_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXD02845.1"
                     /translation="PVVMAHPGFWQRDLDTGLDYRRVVHGEQRTTIFRPLPTAGVVHG
                     RSSVVGVADKGEGRGALAEYQRELRDAAGDLLAISSQTLFYRGDGGIGSAGTMPAPLR
                     DTPERAPDLVAEQQTLPQQALLYRLSGDYNPLHADPAAARQGGFDRPILQGLATYGAA
                     CFMLITALGCAPEAVRRLDCRFKSPAYPGDRFELRIWREPGGASFELREPQRGVVLLG
                     NGFMELSE"
BASE COUNT          373 a          795 c          914 g          407 t
ORIGIN      
        1 catccgatcg gggcgagcgg ggcgcgcatt ctcgtcacgc tggtagcggc gttgcgggct
       61 cgcggcctgc gccgcggtct cgcctcgatc tgcatcggcg ggggcgaggc gacggctgtg
      121 gcggtggagt gcgcggaagc ctaggcagag ttgaatttgt ccacgccgcc caggcggcgg
      181 tcagattgcc ccggcttcgg ccagagccgc gatctccgcc tcgtcggcgc ccaggacgga
      241 gcgcaggata tcgttggtat tctcgcccag gaggggcggg gccatgcgat attcgggcgg
      301 gctggcggag agcttcagcg ggctgcccat ggcgcggatc gggccgatga ccgggtgctc
      361 catggtgacc accatgtcgc gcgccagcgt ttgcggatct gccgccacgt cggacatctc
      421 gttgatcggc ccggtggaga tcttcgcttc gttaagcagt ttcacccagt gggcgcgggg
      481 ttgccggatc acgatcgcgg ccacttcggc cttgatcgcc gcgcggttgg cgacccgggc
      541 gcccatgctc ttgtagcgcg gatcgtcctt ccaatcgggg cggccgagaa cttcggcgag
      601 gcgcacgaat tcacggtcgt tgaaggtggc gatgatcacg tagccgtcgc tggcctcgta
      661 gatgccatag ggcacggcgc tggggtggtc attgccggtc cggccgaggt ggacgcctgc
      721 gttgagccag ctggaggcgg cgttgagcat ggcggcgagc tgggaatcgt aaagcgcgat
      781 gtcgatgttc tggccttcgc ccgtggcatc gcgatggcgc agcgcggcga ggatgccgat
      841 cgtggcgttc atgcccccga agatatcgac gacggggatg cccacccgga tgggtccggc
      901 gccgggctcg ccatcggcaa ggccggtcac gctcatcaga ccgcccatgc cctgcaccag
      961 gtagtcgtag cccgagcgtg aagaataggg acccgtctgg ccgaagccgg tgagcgaaca
     1021 gtaaatcagg ccgggattgc gctggcgcag ctggtttgcg cccaggccat agcggtccag
     1081 cgtgccgggg cggtaattct ccagcagcac gtcgcaccga tcggcgaggc gacggacgag
     1141 gtctgccccg gcctcggtgg cgaagtccac cgcgatggag cgcttgttgc ggttgaccgc
     1201 ctggtaatag gtggaatccg gcgtttcgat gccttcgtgc tcaggcatcc acggggggcc
     1261 gacgcgccgg ctttcgtcac cctcgccctt gcgttcgacc ttgatcacat ccgccccaag
     1321 atcgccgagc gtctgcccgg ccagcgggcc ggcgatgacg cgcgtcatgt cgaggacgcg
     1381 gatgcccgtc agcggcccgg ccatcagccg agatccccct gctcgccggc cacgggttcg
     1441 aacagcacca gcggtgtgtc gccgctctgg tcgatccggg cgcgcaccct gaggccgatc
     1501 ggtgcatccg gcgcggcgga gactacccgg ctcatcattc gcgggccttc atcgagatcg
     1561 accaggacta cgttatacgg ggcctccggc gcgcgcgggt agatcgtggt ggtggcatag
     1621 accgtgccga gcccggatgc ctcgacccat tcgagatcgc gcgcgccggt acccggttcg
     1681 gccacgcgcg ggtagaagac gtggcggccg ctggaccggc tgcgctggat catgaaccgg
     1741 ccctgccgca ggaattcggt gaattcgcgg tcggggcggg ggatcgtcat atcggcggga
     1801 tcgagggtca ttcggacagt tccatgaagc cgttgccaag cagtaccacg ccgcgctgcg
     1861 gctcgcgcag ttcgaaactg gcgccgcccg gttcgcgcca gatgcgcagt tcgaagcggt
     1921 cacccgggta ggcgggcgac ttgaaccggc agtcaagccg ccgcacggcc tccggggcgc
     1981 agcccagcgc ggtgatcagc atgaagcagg ccgcgccgta agtggcgagg ccctgcagga
     2041 tcgggcggtc gaaccctcct tgccgcgcgg cggcgggatc ggcgtgcagg ggattgtagt
     2101 cgccgctgag gcgatagagc agggcctgtt gcggcagggt ctgctgctcg gccacgagat
     2161 cgggggcgcg ttcgggcgta tcacgcaagg gtgcgggcat tgttccggcc gagccgatcc
     2221 cgccgtcgcc gcggtagaac agcgtctggg aagatatggc cagcagatcg cccgccgcgt
     2281 cgcgcagttc gcgctggtat tcggcgagtg cgccacggcc ctcgcccttg tcggccacgc
     2341 cgacgacaga ggatcggcca tggaccacgc cggccgtggg cagggggcgg aagatggtcg
     2401 tgcgctgctc accatgcacc acgcgccggt aatcgagccc ggtgtcgaga tcgcgctgcc
     2461 agaacccggg atgggccata accacaggg
//
LOCUS       DATNBV010000250         4498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3981094,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000250 DATNBV010000000
VERSION     DATNBV010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3981094"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..947
                     /gene="rfbD"
                     /locus_tag="VN627_06015"
     CDS             45..947
                     /gene="rfbD"
                     /locus_tag="VN627_06015"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HXD02846.1"
                     /translation="MRIVVSGVAGQVARSLVERGMALGHVVLPLGRPHLDLASGEAEA
                     IRAVLEMTRPEAIVSAAAYTAVDKAESEPELAFAVNAIGAGHVAEAAAALNVPLVQLS
                     TDYVFSGDGSRAWREDDPTGPQGVYGASKLAGERAVLAACPNSAVLRTAWVYSPFGSN
                     FVKTMLRLAETRDEVGVVADQIGNPTSALDIADGVLAVVANLIGRADPALRGVFHMAG
                     AGEASWADLAEAVFAASAVRGGPAAQVRRIATSDYPTPARRPANSRLDCTRLAGIHGV
                     RLPQWRGSLETVIDRLVSGEGMEA"
     gene            944..1813
                     /gene="rfbA"
                     /locus_tag="VN627_06020"
     CDS             944..1813
                     /gene="rfbA"
                     /locus_tag="VN627_06020"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HXD02847.1"
                     /translation="MKGIILAGGSGTRLYPMTMAASKQLMPVYDKPMIYYPLSTLMLA
                     GIREVLVITTPHDAPAFQGLLGDGSQWGMAISYAVQPEPRGLAQAYTIGADFVAGGPS
                     CLVLGDNIFFGHGLPEQLASARARTTGATVFAYRVADPERYGVVEFDSQMRARSIAEK
                     PLAPQSNWAVTGLYFYDAQVVDIAANLAPSARGELEITDVNAAYLARGQLNVELMGRG
                     YAWLDTGTPDSLLEAAEFVATLERRQGMKIACPEEIAFASGFIDAAGLERCIARHGKS
                     AYGAYLAGLLKGG"
     gene            1910..2857
                     /locus_tag="VN627_06025"
     CDS             1910..2857
                     /locus_tag="VN627_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HXD02848.1"
                     /translation="MSRQADPLFNKLVLLMGGDGFLGTHVAQELLARGARLRVASRHV
                     ERAFRVKPLGNLGQVQFVRADICRTEHHAALVAGVDAVVNLVGAFAGDLDRLHVKAPA
                     ALAAAAQAAGVEAFVQVSANGANADSPVDYARTKAEGEAAVLAAFPRATVLRPSVIFG
                     PDDKFVNMFAGLIAGLPALPVFAPDAKLQPVFVDDVACAIANALGDPGKLGGKTFEAV
                     GPEVLTMLALNHKIAAAQGRKRLFVPLPEAVSSVIAALPGTPISRDQLALLRAGNVGQ
                     GLPGLKALGVAARPLSLFLDRWLVRYRVHGRFGDKRVTG"
     gene            complement(2980..3651)
                     /locus_tag="VN627_06030"
     CDS             complement(2980..3651)
                     /locus_tag="VN627_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXD02849.1"
                     /translation="MWQLHQFPLCPFSRKVRLHLAEKGVPFELWRENPWEQRDEFLDQ
                     NPAARTPVITNAERGITLVDSRAICEYFEETVDKNPLINGTAQMRAEIRRLVALFDEN
                     FFGDVTGPLLHERMKKRLVYRQSPDSRVLREAMKLAHEHLYYIDYLVDTRPWLAGATM
                     SLADLAAAAQISVADYLGGLDWTGHEQAKGWYSVFKSRPSFRPLLAERMEVIQPPSHY
                     ADVNW"
     gene            complement(3732..4091)
                     /locus_tag="VN627_06035"
     CDS             complement(3732..4091)
                     /locus_tag="VN627_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02850.1"
                     /translation="MNSRFLLLPLLLAATPLAAAPRPLTGEERMGLTCAGAFAMVAAG
                     QARGDAAMGQYPPLAQRGREYFVRLAAQLMDDAGLDQAGIRAAAEAQAAPLRRTGVAP
                     VMPSCLRALDEALPAAR"
     gene            4234..>4498
                     /locus_tag="VN627_06040"
     CDS             4234..>4498
                     /locus_tag="VN627_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXD02851.1"
                     /translation="MFSHLTIGSNDLDKAQKFYDSLFTAVGGNPGMRMDTRIMYAHND
                     GLLLVTTPLNGEPATHGNGGTIGLAMNSPEEVEAWHAAGIANGG"
BASE COUNT          676 a         1468 c         1627 g          727 t
ORIGIN      
        1 ggcgagcggc tggggctggc cggcgcagcg cggggctgaa acggatgcgg atcgtcgtca
       61 gcggggtggc tggccaggtc gcccggagcc tggttgagcg gggcatggcg ctgggccacg
      121 tggtcctgcc gctgggccgg ccgcatctcg atctggcatc cggcgaggcc gaggcgatcc
      181 gcgcggtgct ggagatgacg cggcccgagg cgatcgtttc ggcggcggca tacacggcgg
      241 tggacaaggc cgagagcgag ccggagctgg cctttgccgt gaatgcgatc ggggcggggc
      301 acgtggcgga agctgcggct gcgctgaacg tgccgctggt gcagctttcg accgactatg
      361 tgttctccgg cgatgggagc cgcgcctggc gcgaggacga tccgacgggg ccgcagggcg
      421 tctacggcgc gagcaagctg gcgggggagc gggcggttct ggcggcctgc ccgaacagcg
      481 cggtgctgcg cacggcctgg gtctacagcc cgttcggcag caatttcgtg aagaccatgc
      541 tgcgcctcgc cgaaacgcgc gacgaggtgg gcgtcgtggc cgaccagatc ggcaatccga
      601 cctctgcgct cgacattgcc gacggcgtgc tggccgtggt ggccaacctg atcgggcggg
      661 cggacccggc gctgcgcggt gtgttccaca tggccggcgc cggcgaagcg agttgggcgg
      721 atcttgccga ggcggtgttt gccgccagcg ccgtgcgggg cggtccggcg gcgcaggtgc
      781 ggcgcatcgc cacgagcgac tatccgaccc cggcgcgccg gcctgccaat tcgcggctgg
      841 attgcacgcg actggccggg attcacggcg tgcgcctgcc gcaatggcgc ggttcgcttg
      901 aaaccgttat cgaccggctg gtgagcgggg aaggaatgga agcatgaagg ggatcatcct
      961 cgccggcggc agcggcacgc ggctctatcc gatgaccatg gctgcctcga agcagctgat
     1021 gccggtctac gacaagccga tgatctacta tccgctctcc accctgatgc tggccggcat
     1081 tcgcgaggta ctggtcatca ccacgccgca cgatgcgccc gcgttccagg gcctgctggg
     1141 cgacggatcg caatggggca tggccatctc ctatgccgtc cagcccgagc ccagggggct
     1201 tgcccaggcc tataccatcg gcgcggattt cgtggccggg gggccaagtt gcctggtgct
     1261 gggcgacaac atcttcttcg gccacggcct gccggaacag ctggcctcgg cccgggcgcg
     1321 gaccaccggg gcgactgtct tcgcctaccg ggtggccgat cccgagcgct acggcgtggt
     1381 ggaattcgat tcccagatgc gcgcccgctc gatcgcggag aagcccctcg ccccgcagtc
     1441 caactgggcg gtcaccggcc tctatttcta cgacgcgcag gtggtcgaca tcgccgccaa
     1501 tcttgcgccg tcggcccggg gcgagctgga gattaccgac gtcaacgccg cctatctcgc
     1561 gcgcggccag ctcaacgtcg agctgatggg gcgcggctat gcctggctcg ataccggtac
     1621 gcccgacagc ctgctggaag ccgccgaatt cgtcgccacg ctggaacggc ggcagggcat
     1681 gaagatcgcc tgcccggagg aaatcgcctt cgcctccggc ttcatcgacg cggcggggct
     1741 ggagcggtgc atcgcgcggc acggcaagag cgcctatggg gcctatctcg ccggcttgct
     1801 caagggcggg tgagccgcca gggttggatg gcaccgcctt gcccggccag cccgcctgtg
     1861 ccaaaggcgg cgcggcttct tttccgcaaa tcgaggactt gccagaccca tgtcgcgcca
     1921 ggctgatccg ctcttcaaca agctcgtgct gctcatgggc ggcgatggtt tcctcggcac
     1981 ccacgtggcg caggaactgc tggcgcgcgg cgcgcggctg cgagtggcaa gccggcacgt
     2041 cgaacgcgct ttccgggtga aaccgctggg caatctcggc caggtccagt tcgtccgcgc
     2101 cgacatctgc cggaccgagc atcatgccgc gctggtcgcc ggcgtggacg cggtggtgaa
     2161 ccttgttggc gcctttgccg gtgatcttga ccggctgcac gtcaaggccc ccgccgcgct
     2221 ggcggcagcg gcgcaggcgg cgggcgtgga agccttcgtg caggtttcgg ccaacggcgc
     2281 gaatgccgac tcgccggtcg actatgcccg gaccaaggcc gagggtgagg ctgccgtgct
     2341 ggccgccttc ccgcgcgcga cggtgctgcg tccctcggtg atcttcgggc cggacgacaa
     2401 gttcgtgaac atgttcgccg ggctgatcgc cggcctcccc gccctccccg tcttcgcgcc
     2461 ggacgcgaag ctccagccgg tcttcgtcga tgacgtggcc tgcgccattg ccaatgcgct
     2521 tggcgatccg ggcaagcttg gcggcaagac cttcgaggca gtcggccccg aagtgctgac
     2581 catgctggcg ctgaaccaca agatcgccgc cgcacagggc cgcaagcggc tgttcgtgcc
     2641 gctgcccgag gccgtgtcga gcgttattgc cgccctgccc ggcacgccga tctcgcgtga
     2701 tcagctggcc ctgctgcggg ccggcaatgt gggccagggc ctgcccggcc tgaaggcgct
     2761 gggcgtggcc gcgcggccgc tgtcgctgtt tcttgaccgg tggctggtgc gctatcgcgt
     2821 gcatgggcgg ttcggggaca agcgggtaac cggctgacgt cgtcccggac ttgatccggg
     2881 accgctgccc aacgcgaagc tgcaagcgcg cctgctggca agcccccgcc tgaaggttcg
     2941 accccggtcc cggatcaagt ccgggacgac atcagcatgt caccagttca catccgcata
     3001 gtggctcggc ggctggatca cttccatgcg ctctgccagc agcgggcgga agctggggcg
     3061 gctcttgaat accgagtacc agcccttggc ttgctcatgc ccggtccagt cgagcccgcc
     3121 gaggtaatcg gcgactgaaa tctgcgcggc ggcggcgaga tccgccaggc tcatggtggc
     3181 gccggcaagc caggggcgcg tgtcgaccag atagtcgata tagtagaggt gctcgtgggc
     3241 gagcttcatc gcctcgcgca ggacccggct gtcgggcgac tggcggtaga ccaggcgctt
     3301 cttcattcgt tcgtgcagga gcggccctgt cacgtcgccg aagaaattct cgtcaaacag
     3361 ggcgacgagg cggcggatct cggcccgcat ctgggcggtg ccgttgatca gcgggttctt
     3421 gtcgactgtt tcctcgaaat attcgcagat cgcccggctg tcgacgaggg tgatcccccg
     3481 ttcggcattg gtgatgaccg gtgtgcgcgc cgccgggttc tggtcaagga attcgtcacg
     3541 ctgttcccac gggttttcgc gccacagctc gaagggcacg cccttttcgg cgaggtgcag
     3601 gcgaaccttg cggctgaacg ggcagagggg gaactgatgg agttgccaca tgggccgggg
     3661 tcatgccgca ggggtggcga ggagatcaac cgggcggggc aggtttttgc ggtgcataac
     3721 ccccggctgc atcatcgggc ggcgggcagc gcctcatcca gcgcgcgcag gcacgagggc
     3781 atgaccgggg cgacgccggt gcgacgcagc ggtgcggcct gcgcctcggc ggcggcgcgg
     3841 atgccggcct ggtcgagccc ggcatcgtcc atcaactggg cggcgaggcg cacgaagtat
     3901 tcgcggccgc gctgggcgag cggcgggtat tgccccatgg ccgcatcgcc gcgcgcctgc
     3961 ccggcggcaa ccatggcgaa ggctccggcg caggtcaggc ccatgcgctc ctccccggtc
     4021 agcgggcggg gcgcggcggc gagcggcgtg gcggccagca gcaggggcag gagcaggaaa
     4081 cgcgagttca tcaccgcgtc gtagcgcgcg caatctgaac agcaagtatc tgatcgcgcg
     4141 cacagctatc tgattgcgcg cacagggccg ttgacgcgcg ggacgtagcg gctactcatc
     4201 cggcaagagc caacaataca agggagaaag cgcgtgttct cgcacctgac aatcggcagc
     4261 aacgatctcg acaaggcgca gaagttctac gattcgctgt ttacggcggt cggcggcaat
     4321 cccggcatgc gcatggatac ccgcatcatg tatgcccaca acgacgggct gctgctggtg
     4381 accacgccgc tcaacggcga accggcgacc cacggcaacg gcggcacgat cggtctggcg
     4441 atgaattcgc ccgaggaagt cgaggcttgg cacgcggccg gcatcgccaa tggcggca
//
LOCUS       DATNBV010000251         2205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2368089,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000251 DATNBV010000000
VERSION     DATNBV010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2368089"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VN627_06045"
     CDS             <1..626
                     /locus_tag="VN627_06045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HXD02852.1"
                     /translation="QLVNAVNLPVQNALNFPAAILQRPFFDASADPAFNYGAIGAVIG
                     HEISHSFDDNGAAFDATGKLRNWWTPADLARFTKNGKALADQYDIYSPFPGIHVNGRQ
                     TLGENIADLAGLAAAYDAYRASLKGKEPPVIDGFTGDQRFFIAFAQSWATKMREEALR
                     GRIVRDGHAPGQYRALTVRNIDAWYRAFDVQPGDKLYLAPEERVRIW"
     gene            complement(630..953)
                     /locus_tag="VN627_06050"
     CDS             complement(630..953)
                     /locus_tag="VN627_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02853.1"
                     /translation="MDGPLEWFGAIGAIVAAALIAADLGRRVTGWAFVLFVAVSVAWV
                     ASGLIHRTWPLVIQNGLLLAVNGWGVWQFLLSRRKKREIELQEEIAERAERLADRQEA
                     NEARA"
     gene            1055..2140
                     /locus_tag="VN627_06055"
     CDS             1055..2140
                     /locus_tag="VN627_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HXD02854.1"
                     /translation="MKAAVFREVGKPLAVENIRIDKPGPHEVLIRTVASGLCHSDLHF
                     IDGVIPAKFPVVLGHESAGIVEQVGSEVRTVRPGDHVITCTNSFCGHCSQCLTGHANL
                     CDSPETRRGEAETPRLSDAAGAITHFANVSGFAEQIITHEHGVVAMRRDMPFDRAALI
                     GCAVSTGFGAVVNAVKLQPGETIVIIGCGGVGLSAINSAAIAGAGRIIAIDLAAEKRQ
                     LATLFGATDFICAAETDPVEAVIELTGGGAPHVLECIGSKRTAEQAFAMLARGGMATI
                     LGMIAPGVNLEIPGAHFIAEKKIQGCLMGSSRFPVEMPRLVDFYLSGRLKLDDLISRR
                     ISLEQVNEGFDEMRGGKLARSVIMFDH"
BASE COUNT          388 a          767 c          685 g          365 t
ORIGIN      
        1 cgcagctggt caatgccgtc aacctgccgg tccagaatgc gctgaacttc cccgccgcca
       61 tcctccagcg cccgttcttc gacgcctcgg ccgatccggc cttcaattac ggcgcaatcg
      121 gcgcggtcat cggccacgag atcagccact cgttcgacga taacggcgcg gccttcgatg
      181 ccaccggcaa actgcgcaac tggtggaccc cggctgacct cgcccgcttc acgaagaacg
      241 gcaaggcgct ggctgaccag tacgacatct attcgccatt ccccggcatt cacgtgaacg
      301 gccggcagac cctgggcgag aacatcgccg accttgcggg cctcgccgcc gcctatgatg
      361 cctatcgcgc ctcgctcaaa ggcaaggagc cgccggtgat cgacgggttc accggcgacc
      421 agcgcttctt catcgccttc gcgcagtcct gggccaccaa gatgcgcgag gaggccctgc
      481 gcggccggat cgtgcgcgat ggccatgcgc cgggccagta ccgcgcgctg accgtgcgca
      541 acatcgatgc ctggtaccgc gccttcgatg tccagcccgg cgacaagctc tacctcgcgc
      601 ccgaggagcg ggtgcggatc tggtaaggct caggcgcggg cttcgttcgc ctcctgccgg
      661 tcggccaggc gctcggcccg ctcggcgatt tcctcctgca gctcgatctc gcgcttcttt
      721 cgccggctga gcaggaattg ccatacgccc cagccgttta ctgcaagcag caagccgttc
      781 tggatcacca gcggccaggt tcgatgaatc aggccggagg caacccaggc caccgagacg
      841 gcgacgaaca ggacaaaggc ccagcccgtc acccgtcggc caaggtcggc ggcaatcagc
      901 gcggcggcaa caatggcgcc aatcgcgccg aaccattcca gtggaccatc catccccaag
      961 gaagcgccgg tatcggatat gcgttcccgc gttgggggga ccttgcggca gcacagggct
     1021 gtgtcactct ggccgcaagg caaggagaga agccatgaag gcagccgtat ttcgcgaagt
     1081 gggcaagccg ctcgccgtcg agaatatccg catcgacaag cccggcccgc acgaggtcct
     1141 gatccgcacc gtcgcctcgg gcctgtgcca ttcggacctt cacttcatcg atggcgtcat
     1201 tcccgccaaa ttcccggttg tgctgggcca cgaaagcgcc ggcatcgtcg agcaggtcgg
     1261 ctccgaagtg cgaacggtcc gcccgggcga tcatgtgatc acctgcacca actcgttctg
     1321 cggccattgc agccaatgcc tgacaggcca cgccaacctc tgcgattcgc ccgaaaccag
     1381 gcggggtgaa gccgaaacgc cgcggctgag cgatgccgcc ggcgcaatca cccacttcgc
     1441 caacgtctcc ggctttgccg agcagatcat cacccacgag cacggcgttg tcgccatgcg
     1501 gcgcgacatg ccgtttgacc gcgcggccct gatcggctgc gccgttagca ccgggttcgg
     1561 cgccgtggtc aatgcggtca agctgcagcc gggcgaaacc atcgtgatca tcggctgcgg
     1621 cggggttggc ctgtcggcca tcaattccgc cgccattgcc ggtgccgggc ggatcatcgc
     1681 gattgacctt gccgcggaga aacgccagct cgcgaccctg ttcggggcga ccgacttcat
     1741 ctgcgcggcg gaaaccgatc cggtcgaagc ggtgatcgaa ttgaccggtg gcggcgcgcc
     1801 tcacgtgctc gaatgcatcg gctccaagcg gacggcggaa caggccttcg ccatgcttgc
     1861 gcgcggcggc atggccacga tcctcggcat gatcgcgccc ggcgtcaatc tcgagatccc
     1921 gggcgcgcac ttcatcgccg agaagaaaat ccagggctgc ctgatgggct ccagccgctt
     1981 cccggtggaa atgccgcgcc tggtcgattt ctacctgagc ggccggctca agctcgacga
     2041 tctcatctcg cgccggatct cgctcgaaca ggtgaacgaa gggttcgacg agatgcgcgg
     2101 cggcaagctc gcccgctccg tcatcatgtt cgatcactag gcggcgaact tagaggagaa
     2161 gcagcgatgc catcggtcgt tgtcgtcggc acgcggttcg gcgcc
//
LOCUS       DATNBV010000252         3851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2069824,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000252 DATNBV010000000
VERSION     DATNBV010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069824"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VN627_06060"
     CDS             <1..1051
                     /locus_tag="VN627_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HXD02855.1"
                     /translation="TTPLAPEAREAMLPWLAGPDSPGFANPHSPHRPGRQAAAAVEIA
                     REQVAALLPAGGRVIFTSGATEALNLAILGSGRRRLAVSAIEHAAVLETARFADPQAA
                     ILPVDAAGLVDPAIVLPDGVGLVAVMQVNNEIGTIQPVERLAERARAAGALFLCDAVQ
                     GAGKLAPPEAADLIAISAHKLHGPKGIGALWVRDGVDLAPQVHGGGQEGGLRSGTLSP
                     ALCAGFGAAAQLAVQRRDDDAANVEALWEAARNALPGFTLNGSSDQRWRGNLNIRREG
                     LDIARLMSDLRDIAFSAGSACASGSGKPSYVLRAIGLSDKEARSSIRLGFGRYSRVHE
                     VTEACARIAAAAEAQ"
     gene            1077..1409
                     /locus_tag="VN627_06065"
     CDS             1077..1409
                     /locus_tag="VN627_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXD02856.1"
                     /translation="MVSVCFITAEGEKVHRDAEPGARLLEVGQAAGMPLEGTCEGQMA
                     CSTCHVVIAAEWFAKLAPASEDEEDMLDLAAGIARTSRLSCQIVLTNDLDGIEVHIPG
                     EARDMQRR"
     gene            complement(1406..1930)
                     /locus_tag="VN627_06070"
     CDS             complement(1406..1930)
                     /locus_tag="VN627_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HXD02857.1"
                     /translation="MPKRVLILATDGFEQSELIEPRRALEAAGIETVLASPKRGEIKG
                     WHHHDWGESVRVDAQLGEVEAEDFDALLLPGGQMNPDSLRLEDEVICLIEEFDEAEKP
                     IAAICHGPWLLVEADIVEGRAVTGWPSIMTDLENAGAQVFDEEVIVDGNLITGRKPED
                     IPAFSRALIDALTD"
     gene            2034..>3851
                     /gene="parC"
                     /locus_tag="VN627_06075"
     CDS             2034..>3851
                     /gene="parC"
                     /locus_tag="VN627_06075"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HXD02858.1"
                     /translation="MPSTTIDEPDPFDAIVDAPFDSALSERYLVYALSTITARSLPDL
                     RDGLKPVHRRLLWAMRQLKLDPTSAYKKSARVVGDVIGKYHPHGDSSVYDAMVRLAQD
                     FSLRYPLVEGQGNFGNIDGDNAAAYRYTEARLTRTAIRLMAGLDEGTVDFVPTYNGED
                     EEPELFPGLFPNLLANGSSGIAVGMATNIPSHNVAEIVDATLLLIDNPGVEHAQLMEV
                     FHGPDFPTGGVVVDSPAQISAAYESGKGPIRLRGRFSTGREESGEWEETGIEKLGGGQ
                     WQLVLSEVPYMVQKGKLIEQIATLIADRKLPILEDVRDESDEQIRIVFVPKSRNVDPD
                     LLKESLYRLSDLEVRFGLNLNVLDAHRTPGVLGLKLLLQEWTISQIDILIRRSRHRLE
                     KIAARLELVEGYIIAYLNLDRIIEIIRTEDEPKPVMMAEVGLTDRQAEAILNMRLRSL
                     RKLEEMELRKERDDLLAEQEELHKLLDSPARQRTRLKRDLAALRKEYGPETALGRRRT
                     TLAEAAPTREFSMDAMIEKAPVTVIISAKGWIRGASGHDPLDREYKFKEGDGPAFALH
                     AQTTDKLLVACDNGRFYTLGCDKLPSARGFGEPIRTMVDI"
BASE COUNT          664 a         1337 c         1221 g          629 t
ORIGIN      
        1 taccacgccg ctggcccccg aggcgcgcga ggccatgctg ccgtggctcg ccggacccga
       61 ttcgccgggc ttcgccaatc ctcacagccc gcaccgcccc ggccgtcagg ccgctgccgc
      121 cgtggagatc gcgcgcgaac aggtcgccgc gctgcttccg gcgggcggca gggtgatctt
      181 caccagcggg gcaacggagg cgctcaacct cgccatcctc ggctcggggc ggcgccgtct
      241 ggcggtcagc gccatcgagc atgccgcggt gctggaaacc gcccgcttcg ccgatccgca
      301 ggccgccatc ctgccggtcg atgccgccgg gctggtcgat cccgcaatcg tcctccccga
      361 tggggtcggc ctcgtcgcgg tcatgcaggt caacaacgag atcggcacga tccagccggt
      421 cgagcgcctg gccgaacggg cgcgggcggc gggcgccctg ttcctgtgcg atgccgtcca
      481 gggcgcgggc aagctggctc cgcctgaagc agccgacctg atcgccattt ccgcgcacaa
      541 gctccatggc cccaagggca tcggcgccct gtgggtgcgc gatggggtcg atctggctcc
      601 gcaggtccac ggcggcgggc aggagggcgg cctgcgctcg ggcacgctca gccccgcgct
      661 ctgtgccggg ttcggcgcgg cggcgcagct cgcggtgcag cgccgcgatg acgacgccgc
      721 caatgtcgaa gcgctgtggg aagcggcgcg taatgcgctg ccgggattca ccctcaacgg
      781 cagctccgac cagcgctggc ggggcaacct caacatccgc cgcgaaggtc ttgatattgc
      841 cagattgatg tccgatctgc gtgatattgc cttttctgcc ggctccgcct gtgccagtgg
      901 atcgggcaag cccagctacg tgctgcgggc aatcgggctc agcgacaagg aagcccgaag
      961 ctcgatccgg ctaggcttcg gccgttattc gagggtgcat gaggtcactg aggcctgcgc
     1021 gcggatcgct gcggcggctg aagcgcagta ggctggctgc agggagccca cgcaagatgg
     1081 tatcggtctg tttcatcacc gccgagggcg aaaaggtcca ccgggacgct gaacctggcg
     1141 cgcgcctgct cgaggtgggg caggctgcgg gcatgccgct cgagggaacc tgcgagggcc
     1201 agatggcctg ttcgacctgc cacgtggtga tcgccgccga atggttcgcc aaactcgcgc
     1261 cagcatctga ggacgaggaa gacatgctcg atctcgccgc cggcatcgcc cggaccagcc
     1321 ggctctcctg ccagatcgtc ctgacgaatg atctcgatgg catcgaagtc cacatcccgg
     1381 gagaggcccg ggacatgcag cggcgctaat cggtcaaagc gtcgatcagg gcgcgcgaga
     1441 aggcgggaat gtcctcgggc ttgcgcccgg tgatgaggtt gccatcaacg atcacttcct
     1501 cgtcgaacac ctgcgccccg gcattttcca ggtcggtcat gatcgatggc cagccggtca
     1561 cggcgcggcc ctccacgata tcggcctcca ccagcagcca cggcccgtgg cagatcgccg
     1621 cgatcggctt ctccgcctcg tcgaattcct cgatcaggca gatcacttcg tcttccagcc
     1681 gcaggctgtc ggggttcatt tgcccgcccg gcagcagcag ggcgtcgaag tcttccgcct
     1741 ccacctcgcc aagctgggca tcgacgcgca cgctctcgcc ccagtcgtgg tgatgccagc
     1801 ccttgatctc gccgcgcttg gggctggcga ggacggtctc gatcccggct gcctccagcg
     1861 ccctgcgcgg ttcgatcagc tcggattgct cgaatccgtc ggtagcgagg atgagtacgc
     1921 gcttgggcat ggaattctcc ccatgggact gtcccgaccc tgcaaatgag cgggggatgg
     1981 acattgttcc cgggattccc ttggtccacc cggcttgcta gggctgggaa accatgccga
     2041 gcaccacgat cgatgaaccc gatccgtttg acgcaatcgt cgacgcgccg ttcgacagtg
     2101 ccctgtctga acggtacctc gtctatgcat tgtcaacgat tacagcgcgt tcactgccgg
     2161 acctgcgcga tggcctgaag ccggttcacc ggcggctgct atgggccatg cgccagctga
     2221 agctcgaccc gacctccgcc tataaaaaat ccgcgcgcgt ggtgggcgat gtcatcggca
     2281 aataccatcc gcacggcgat tccagcgtct acgacgcgat ggtccgcctc gcgcaggatt
     2341 tctcgctgcg ctacccgctc gtcgaagggc agggcaattt cggcaatatc gacggcgata
     2401 acgctgccgc ctatcgctac accgaagccc gcctgacccg taccgcgatc cgcctgatgg
     2461 ccgggcttga tgaaggtacg gtcgatttcg tccctaccta caacggcgag gacgaggagc
     2521 ctgagctgtt ccccggcctc ttccccaatc tcctcgccaa tggctccagc ggcatcgccg
     2581 tgggcatggc gaccaacatc cccagccaca acgtcgccga gatcgtcgac gccaccctgc
     2641 tgctgatcga caatcccggg gtcgagcatg cccagctgat ggaagtgttc cacggccccg
     2701 attttcccac cggcggcgtg gtggtcgaca gtccggccca gatcagcgcc gcctatgaaa
     2761 gcggcaaggg cccaatccgc ctgcgcgggc ggttctccac cggccgcgag gagagcggcg
     2821 agtgggaaga gaccgggatc gagaagctgg gcggcggcca gtggcagctg gtgctctccg
     2881 aagttcccta catggtccag aagggcaagc tgatcgagca gatcgccacg ctgatcgccg
     2941 acaggaagct gcccatcctc gaagacgtgc gcgacgaaag cgacgagcag atccgcatcg
     3001 tcttcgtccc caagagccgc aacgtcgatc ccgatctgct caaggaaagc ctctatcggc
     3061 tgagcgacct ggaggtgcgc ttcgggctca acctcaacgt gctcgacgcc catcgcaccc
     3121 ccggcgtgct cggcctcaag ctgttgctgc aggaatggac gatctcgcag atcgacatcc
     3181 tgatccgccg cagccgtcac cgcctggaaa agatcgccgc ccgcctggaa ctggtcgaag
     3241 gctacattat cgcctacctc aacctcgacc ggatcatcga gatcatccgc accgaggatg
     3301 agcccaagcc ggtgatgatg gccgaggttg ggctgaccga tcgccaggcc gaggccatcc
     3361 tcaacatgcg cctgcgctcc ttgcgcaagc tcgaggaaat ggaactgcgc aaggagcgcg
     3421 acgatctcct cgcggagcag gaagagctcc ataagctgct cgacagcccg gcccgccagc
     3481 gcacccgtct gaagcgcgat ctcgccgccc tgcgcaagga atacggcccc gagaccgccc
     3541 tcggtcgccg ccgcaccacc ctcgccgaag ccgcgcccac gcgcgaattc tcgatggacg
     3601 cgatgatcga aaaggcgccg gtcaccgtca tcatctcggc caagggctgg atcaggggcg
     3661 ccagcggcca cgacccgctc gaccgggaat acaagttcaa ggaaggcgac ggcccggcct
     3721 tcgcgctcca tgcgcagacc accgacaagc tcctggtcgc ctgcgacaat ggccggttct
     3781 acaccctcgg ctgcgacaag ctgccctccg cccgcggctt tggcgaaccg atccgcacca
     3841 tggtcgatat c
//
LOCUS       DATNBV010000253         2853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_790203,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000253 DATNBV010000000
VERSION     DATNBV010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790203"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VN627_06080"
     CDS             complement(<1..1122)
                     /locus_tag="VN627_06080"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD02859.1"
                     /translation="MSTVLDREAQSAPRFDYPIYDADHHYYEPPEAFLRHLDPKYHSK
                     FQYVTLANGRTKLVVDGFLSDYIPNPTFNVVAGPGTHEAYYRADNPQGLTLREMTGTP
                     MRSIPAFHNGAAHLKMMDELGYHAAIVFPTLASIIEVRLGHQTELTQALMHSLNTWVA
                     EEYGFGNGHQFPVGAISLCDPAGAVKELEFVLKAGCKQILIRPAAVPLTDGGFCSPAD
                     RRFDNFWRLCEQERVLINNHVSDSGYDRIFREWSGDTRGEMIAFDRAAKKEIFNAMGR
                     AAEDSITSLIVDGLFDRFPGLKLLVTESGSAWIAPLLERMERAYHKMPWEFARSPVET
                     FREHVYVMPFYEDSVSALAEVMPIENICFGSDWPHPEGLV"
     gene            1378..2478
                     /locus_tag="VN627_06085"
     CDS             1378..2478
                     /locus_tag="VN627_06085"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HXD02860.1"
                     /translation="MATLADSALRFAHASHPAPTPPAVRDAALADPGFGKLFSDHMVT
                     IEWSEDAGWHDAVVGPLQPLPLHPAAAVLHYAQEIFEGLKAYRLADGAMALFRPQANA
                     ARFNASAQRLAMPELPEDLFVEACRQLVAVDRDWFPSVEGGSLYLRPFMIATEAFLGV
                     RPAKTYKFIVIASPAGNYFKSGAPAVSIWVSQYTRAAPGGTGAAKCGGNYAASLVPQG
                     EAMAKGHDQVVFLDAEQHKWIEELGGMNLYFVFDDGSLVTPQLTGTILPGITRDSLLT
                     LAREEGLTVREERYSIDQWRADAASGKLVETFACGTAAVVTPVGRVTDPVKGEFVIGS
                     GGPGQLTQKLRGRLVAIQRGEAPDPHGWVVRL"
     gene            complement(2593..>2853)
                     /locus_tag="VN627_06090"
     CDS             complement(2593..>2853)
                     /locus_tag="VN627_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HXD02861.1"
                     /translation="FELLATATGKLSVKALASEVSERLGNTPAIARKSYIHPAVLALV
                     DGQEEWRKRLRLPRATRWLTRYERGLIALLEEAPAAAELLAG"
BASE COUNT          472 a          917 c          966 g          498 t
ORIGIN      
        1 gacgaggcct tcggggtgcg gccagtcgct gccgaagcag atgttttcga tcggcatgac
       61 ttcggcgagg gccgagaccg aatcctcgta gaacggcatg acgtagacat gctcgcggaa
      121 ggtctcgacc gggctgcggg cgaactccca gggcatcttg tgataggcgc gttccattcg
      181 ctcgagcagt ggagcgatcc aggccgagcc gctttcagtg accagcagct tgagaccggg
      241 gaaccggtcg aacagcccgt cgacgatcag cgaggtgatc gaatcttcgg ccgcgcggcc
      301 catggcgttg aagatttcct tcttggccgc ccggtcgaag gcaatcatct caccgcgggt
      361 atcgccgctc cactcgcgga agatgcggtc gtagccggag tccgagacgt ggttgttgat
      421 caggacgcgc tcctgttcgc acaggcgcca gaagttgtcg aaacggcggt ctgccggcga
      481 gcagaagccg ccatccgtca gcggcacggc cgccgggcgg atcaggatct gcttgcagcc
      541 tgccttgagg acgaattcga gctccttcac cgcaccggcc ggatcgcaca ggctgatcgc
      601 gcccaccgga aactggtggc cgttgccgaa gccatactcc tcggccaccc aggtattgag
      661 cgagtgcatc agggcctggg tcagttcggt ctgatggccg aggcgaacct cgatgatcga
      721 ggcgagcgtg gggaagacga tggccgcgtg gtagccaagc tcgtccatca tcttgaggtg
      781 ggccgcgccg ttgtgaaagg cgggaatcga cctcatcggc gtgcccgtca tttcgcgcag
      841 ggtcagcccc tgcggattgt cggcccggta ataggcctca tgggtgccgg ggccggcgac
      901 gacgttgaag gtcgggttgg ggatgtagtc cgagagaaac ccgtccacca ccagcttggt
      961 ccggccattg gccagggtca cgtactggaa cttgctgtga tacttgggat cgaggtggcg
     1021 caggaaggct tccggcggct cgtagtagtg atggtccgcg tcgtagatcg gatagtcgaa
     1081 ccggggcgcg gactgggctt cgcgatcgag gacggtggac atcggaggat ccttgtcgaa
     1141 aaatttgatg atacgtcata ttcggcggtt ccgcaggcga gtccttgatt cagatcaaac
     1201 cgcccccttt gggcgagcaa caccgcggcc gcgccgtcgc aacaggcttg gccttgcgcg
     1261 cgaaaggccc tagatcgctg aacaagggcg atgagccttt tcgaatatta caagaaactt
     1321 gcgcagggcg aaattttcgt tcagggcgag tggacgcagc atacaggatc tttgcccatg
     1381 gcgactctcg ccgattccgc ccttcgtttc gcccatgcct cccaccccgc gccgacgccg
     1441 ccggctgtgc gcgacgcggc gctggccgat cccggtttcg gcaagctttt ctccgatcac
     1501 atggtgacga tcgagtggag cgaagatgcc gggtggcatg atgccgttgt cggtccgctc
     1561 cagccgctgc ctctgcatcc ggcggctgcc gtgctgcact atgcccagga aatcttcgag
     1621 gggctgaagg cctatcgtct cgccgacggc gccatggccc tgttccgtcc gcaggccaac
     1681 gccgcgcggt tcaacgcctc ggcccagcgc ctcgccatgc ccgaactgcc cgaagacctg
     1741 ttcgtcgagg cctgccgcca gctggtggcg gttgaccgcg actggttccc cagcgtcgaa
     1801 ggcggctcgc tttacctgcg ccccttcatg atcgcgaccg aggccttcct cggcgtgcgc
     1861 ccggcgaaga cctacaagtt catcgtcatc gccagcccgg cgggcaatta cttcaagagc
     1921 ggcgcgccgg ccgtttcgat ctgggtcagc cagtacaccc gcgccgcgcc cggcggcacc
     1981 ggagcggcca agtgcggcgg caactatgcc gccagcctcg tcccccaggg ggaggccatg
     2041 gccaagggcc acgaccaggt ggtgttcctc gatgccgagc agcacaagtg gatcgaggaa
     2101 ctgggcggca tgaacctcta cttcgtgttc gacgacggct cgctggtcac cccgcagctg
     2161 acgggcacga tcctgcccgg catcacccgc gacagcctgc tcacccttgc ccgcgaggaa
     2221 gggctcaccg tgcgcgagga gcgctattcg atcgatcagt ggcgggcgga tgcggccagc
     2281 ggcaagctcg tcgaaacctt cgcctgcggc acggcggcgg tggttactcc ggttggccgg
     2341 gtgaccgatc cggtcaaggg cgagttcgtg atcggcagcg gcggcccggg ccagttgacc
     2401 cagaagctgc gcggccggct ggtggcaatc cagcgcggcg aggcacccga tccgcacggc
     2461 tgggtggtgc ggctctaggc cgcgcccgca gagcgtttgg tggagggggc tcagcccgag
     2521 cagctgcgcc tgccggaaca ccccctccgt cagccctgac aggctgccac ctccccgcgg
     2581 gcggggagga gttcatcccg ccagaagttc ggctgccgcc ggcgcttctt cgagcagggc
     2641 gataaggccg cgctcgtacc gcgtcagcca gcgcgtggcg cggggcaggc ggaggcgctt
     2701 gcgccactcc tcctggccat cgaccaaggc caggacggcg gggtggatat aactcttgcg
     2761 cgcgatcgcg ggggtattgc cgagccgctc gctgacctcg ctcgccagcg ccttgacgct
     2821 gagcttgccc gtggcggttg ccagcaattc gaa
//
LOCUS       DATNBV010000254         2847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2370123,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000254 DATNBV010000000
VERSION     DATNBV010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2370123"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1955
                     /locus_tag="VN627_06095"
     CDS             312..1955
                     /locus_tag="VN627_06095"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HXD02862.1"
                     /translation="MRIALLQTNPTAGDIDGNSALIVRGARQARDQGADLVVTPELAL
                     MGYLPRDLLMSRGFIHRSARELSRMAAELADGPPLLVGVATLNPAEMGRPLFNSAVLL
                     RNGAAGSVFHKTLLPTYDVFDEDRYFEPAHGPQVLELNGCRLGISICEDVWNDRDFWK
                     RRRYHEDPIEGLAKIGAHAILNLSASPFTVGKQSLRQDMLGHMAQKYRLPLMIVNQVG
                     GNDDLIFDGRSAAFDSQGKLFVRAKGFEEDLVIVDLTAGAAGSSGIAPDDFQAEAEIW
                     NALVLGVRDYARKTRFRKVLLGLSGGIDSALTAAIAADAMGPENVLGVMMPSRYSSRG
                     SIDDSVEVARNLGIQTLTLPISGIMETYECVLSDAFRGLQPDVTEENIQSRIRGNLLM
                     ALSNKFGSLLLTTGNKSEMSVGYCTLYGDMNGGLAVIADLPKMMVYRVSRWRNGRKPD
                     IPEAILTKAPSAELRPDQTDQDSLPPYELLDQILELHVEQSQSAEEIIAQGFEEATVR
                     RVLRLVRMAEFKRKQAAPVLKVTSRAFGTGWRMPIVRQE"
     gene            1965..2825
                     /locus_tag="VN627_06100"
     CDS             1965..2825
                     /locus_tag="VN627_06100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXD02863.1"
                     /translation="MKTTVTSDQEREFYDSRYAQFLTLPDHALRLDRRVLEGNLDNPA
                     HPFYERRWLYRASMRALEALPLPGRKVLDYGCGPADFGIWMATEGARVTLLDLSPAAI
                     QVGLKRARASGVEVRGIAADASRLAMLEAGEFDLVFACAALHHTLKYPGAVEELARIM
                     KPGARLVLCETWGGNPLLNRARKLHAALIGEAEEQGEDIILSHVQLRALAPHFSDVVV
                     ETRNLLAMAKRLLRGRFERRWARAAVRAFERVDGVILALAPPLRNWCGEAVITARRVP
                     ANEPAGSVSP"
BASE COUNT          501 a          948 c          891 g          507 t
ORIGIN      
        1 cgcggccgct gaagtagcgg agcacggacc cgtaaccgtt caggatctcg agggttccat
       61 cggtggatcc gccgtccgcc acgatatgtt ccaggtgggg gtaatcctgc gacaacacgc
      121 tttcgattgc gaagggcaga tagcgagcca tgttgtagca cggagtaacg atgctgacga
      181 ggcacgctgc ggaatccacg tgagagaggt acactatatc actgtttaca cgtatgttta
      241 cgccctaaat cgagtttaaa cgcccgctgc ctgcatccgg ataaactggt ttgaggaggg
      301 catcttccgg tttgcgcatc gcgctgcttc aaaccaaccc cacggccggt gacatcgacg
      361 gcaactccgc cttgatcgtc cgcggcgccc ggcaggcccg cgaccagggt gcggacctgg
      421 tggtcacgcc ggaactcgcc ctgatgggat atctcccgcg cgacctcctg atgagccgcg
      481 gattcatcca tcgcagcgcc cgggagctca gccgtatggc cgccgaactg gcggacggcc
      541 cgcccttgct ggtgggcgtg gctacgctca atcccgcgga gatgggccgc ccccttttca
      601 atagcgcggt gctgctgcgt aatggcgcgg ccggttcggt cttccacaag actctcctgc
      661 ccacttacga cgtcttcgat gaagatcgct atttcgaacc cgcccacggt ccgcaggtgc
      721 tggagttgaa cggctgccgc ctgggcatca gcatttgcga agacgtctgg aacgatcgcg
      781 acttctggaa gcgccgccgg tatcatgagg accccatcga gggactggcg aagatcggcg
      841 cgcatgccat cctcaacctt tccgcctccc cgtttaccgt gggcaagcag tccctgcgcc
      901 aggacatgct cggccatatg gcgcagaagt accgcctgcc gctcatgatc gtgaaccagg
      961 tgggcggcaa tgacgacctg atcttcgacg gccgcagcgc cgccttcgac agccaaggca
     1021 aactcttcgt ccgcgccaaa gggttcgagg aagacctggt gattgtagac ctcaccgccg
     1081 gcgccgccgg atcgagcggg atcgccccgg acgacttcca ggccgaagcc gaaatctgga
     1141 acgccctggt gctgggggtc cgcgattacg cccgcaagac acgtttccgc aaggttctgc
     1201 tgggcctctc cgggggtatc gactcggccc tgacggccgc catcgccgcc gacgccatgg
     1261 gcccggaaaa cgtgctcggg gtaatgatgc cctcgcgcta ttccagccgc ggcagtatcg
     1321 acgattcggt agaggtggcg cgcaatctcg gcatccaaac cctcaccctg cccatctccg
     1381 gcatcatgga gacctacgag tgcgtgctct ccgacgcctt ccgcggtctc cagcctgacg
     1441 tcaccgagga gaacatccag tcgcgcatcc gcggcaacct gctgatggcc ctttccaaca
     1501 agttcggatc gctgctcctc accaccggga acaaatccga aatgtcggtg ggctactgca
     1561 cgctgtacgg cgatatgaac ggcggcctgg ccgtgatcgc cgacctgccc aaaatgatgg
     1621 tgtaccgggt ctcgcgttgg cgcaatggcc gcaagcccga catccccgaa gccatcctca
     1681 ccaaggcgcc ctcggctgag ctgcggccgg atcagaccga ccaggacagc ctgccgccct
     1741 acgaactgct cgatcagatt ctggagttgc atgtggagca gtcgcagtcg gcagaagaga
     1801 tcatcgcgca ggggttcgag gaggccacgg tgcgccgcgt gttgcggctg gtgcgcatgg
     1861 cggaattcaa acgcaagcag gcagccccgg tgctgaaggt cacctcgcgc gcattcggca
     1921 ccggctggcg gatgcccatt gtgcgccagg aatagcgttc ccctgtgaag accaccgtca
     1981 cctccgacca ggaacgcgag ttttacgaca gccgctacgc gcaatttctg acgctgcccg
     2041 accacgccct gcggctcgac cgccgggtgc tggaaggcaa cctcgacaac cctgcgcatc
     2101 ctttctatga acgccgctgg ctgtaccgcg cctccatgcg ggcgctcgaa gccctgccgc
     2161 tgcctggcag aaaggtgctc gactacggct gcggtcccgc cgatttcggc atctggatgg
     2221 ccaccgaagg cgcccgcgtc acgctgctgg atctgtcccc cgcggccatc caggtgggat
     2281 tgaagagggc ccgcgcttcc ggcgtggagg tccgcggcat cgcagcggac gccagccgcc
     2341 tggccatgct ggaggccggc gaattcgacc tggtattcgc ctgcgccgcc ctgcaccata
     2401 cgttgaaata tcccggcgcg gtggaggaac tggcgcgcat catgaagcct ggcgcccggc
     2461 tggtgctgtg cgagacctgg ggcggcaatc cgctgctcaa ccgcgcccgc aaattgcacg
     2521 ctgctctcat cggggaggcg gaggagcagg gcgaagacat catcctgtcg catgtgcagc
     2581 tgcgggcttt ggcgccgcac ttttccgacg tagtggtgga aacccgcaat ttgctggcga
     2641 tggccaaacg cctgctccgc ggccggttcg agcgccgctg ggcccgggcc gccgtccgcg
     2701 cgttcgagcg cgtggatggc gtcatactcg ccctcgctcc cccgctgcgc aattggtgcg
     2761 gcgaggctgt gattacggcg cgacgcgtgc ctgcgaacga gccggctggg tccgtatccc
     2821 catgacctgt tcaatcgcca tgagggt
//
LOCUS       DATNBV010000255        15436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1428588,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000255 DATNBV010000000
VERSION     DATNBV010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 15436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15436
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428588"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..280
                     /locus_tag="VN627_06105"
     CDS             155..280
                     /locus_tag="VN627_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02864.1"
                     /translation="MKKTEKTVWTAPALRKLGTIGDVAGSNNGIVQNQAPGNPKS"
     gene            559..2334
                     /locus_tag="VN627_06110"
     CDS             559..2334
                     /locus_tag="VN627_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HXD02865.1"
                     /translation="MPGQRARGGGWEPEPCPDGSRAVFHGWFDNLAEIATHLGLAPGA
                     SAARVYGEAVARWGDAADSRIVGTYAAVVAGPDGLRLSRSPWSAPALFYANDDARAMA
                     ASALRALAAGGMERVLNRAALAAMVSFCTEWDDQTFYEGARMVPHGAVVHLRPGAARV
                     VNWYDPQAIPEVRFSRDEDYVAAAREQLDAVIAATLPYARRSGIALSGGLDSAIVAAH
                     VLRQLAPGQTLPSFTFTPHPDWDGSTPPGTFGDERAAVEAFAAMHPALRPLFPDDSDA
                     GYDEAARQFAQAMDGFHRHLHLAAPYQGVWRAAREAGCDLLLTADWGNGNISSDAPWA
                     PVEFLRTGEWEQLVALLRGKRGDTRPLWRRLLARAVLPQLPRPLRRAARRFVHGDASR
                     VRADTRLARPEVMERYGVGPWAGTRLPIDYFESPRTRAENVLCQYEWARVDSGEALTA
                     YEQVHGIRGRDITAYRPLIELCLGMPASQFARDGVDRWLARRMGAGLMPEAQRTNNLY
                     GRHHADWHVRMTPRRERMREDALRLAEHPWLGEWFDTERMLKLIDDWPAETTFDEAIE
                     VPRRYALPLVLNAGDFVNWFEGRNA"
     gene            2327..4123
                     /locus_tag="VN627_06115"
     CDS             2327..4123
                     /locus_tag="VN627_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXD02866.1"
                     /translation="MPDRGGATRGGTSQADPARAGAHLLARAVPRPLLAGVLALGLAA
                     SLTEGVGLMLLVPVLGLVGAGGNALPGSAPGRLAALWRHLGGALPGDIAGLLGLFVLV
                     VALRAALVQGRILAEQALRLAVTNNLRRDLFGALLHADWLWLAGARQGRLLGVVLGTI
                     DRLGAGLQHLQSLFAALVTLAIMLIAALLIDPLPAAALGLGGLLVLAIHVALRRSARR
                     DGDRVNRGYEDYYGFYAERLAMLRQIKSFGAERREADRAGVVAAALRRAQLGFSRGLG
                     LGNFVLQAGTAAVLGLAVWLAVTRWHSSPVTLLPLIAISARAVPLLSVVQSGLQNLAH
                     DRPALAELGACQLEALHYAESESASESAGVPLPPARRELRLEAVSLYHAGREQAALDR
                     LTLTLAAGSMVHLAGESGAGKSTLADVLAGLVSPTSGRLLIDGMAIGPVQRRGWRQRV
                     AYVQQEPVLLHASIRDNLRWAAPGASDAQVEQALREAAAQFVFALPDGLDTVVGERGA
                     SLSGGERQRIALARGLLREPDLLILDEVTSALDAANEAAVAGAIARLRGRMTILIIGH
                     RGALAGLAERRLVMAQGRLTADEASGNEAARA"
     gene            4190..4699
                     /locus_tag="VN627_06120"
     CDS             4190..4699
                     /locus_tag="VN627_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HXD02867.1"
                     /translation="MISSRIAACLALAAVALLPLRAAAAPAAAMTGVSPPAPAGCTGR
                     PSATWIDVSVEGLRSGSGQVAITLYADNSRKFLVKNGSLYVGRVPAQAGTTTGCIFVP
                     QPGVYAIAIYHDENGDGSFNRTAIGFPAEGFGFANNPSTLAGLPSFKSVRLAIPRAGL
                     LTRVRLRYP"
     gene            complement(4708..5454)
                     /locus_tag="VN627_06125"
     CDS             complement(4708..5454)
                     /locus_tag="VN627_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HXD02868.1"
                     /translation="MDAVIIAAGFGSRISALSDSKPLTPVAGIPLIELGIRQLAAAGV
                     TRVVVVTGHEGERVEAFLASLARRTGMAIHAERVSDWSKPNGFSVIAGADRVAALGGG
                     DFLLVMADHMFSAEILRRLAAEGSPERGVTLAVDRRVDNPLVDPDDATWVQTGPDGRI
                     VAIGKTIPVYDAVDCGAFLVTPELPEAIAAAIAAGKGGSLSDGMQVLADRGRAATMEI
                     AGAWWLDVDDPRAHAIAEAEAPAQLNLVSG"
     gene            complement(5480..6580)
                     /locus_tag="VN627_06130"
     CDS             complement(5480..6580)
                     /locus_tag="VN627_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HXD02869.1"
                     /translation="MKPIKVAIIGVGNCASSLVQGVAYYRQNNSAQGLIHEVIGGYGA
                     GDVDFVLGIDVDKRKVGKDVAEAIFAGPNCTAVFQPDVPATGGKVIMGKILDGVADHM
                     TGMGERGFIVADEPEATRESIIAALKASGAEILVNFLPVGSEEATEFYMECALEAGIA
                     VVNCMPVFIASRPEWERRFAEKRIPIVGDDIKAQVGATIVHRVLASLFGARGVNVERT
                     YQLNTGGNTDFMNMLDRQRLGSKKESKTEAVQAMLAQRLEDENIHVGPSDYVPWQKDN
                     KLCFLRLEGNQWGNVPMNLELRLSVEDSPNSAACVMDAIRCCKVALERGEGGALAGPS
                     AYFCKHPPVQFNDDDAARMVEEYIAPAAIAAE"
     gene            6892..7827
                     /locus_tag="VN627_06135"
     CDS             6892..7827
                     /locus_tag="VN627_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXD02870.1"
                     /translation="MSDLATGAGGQQQAEARPRPQRPRELQDALNYYLYHPLAWQLAR
                     LLARTPLTPNMVSVIGGSCVVAAAVVYAQPGWAQFPSGPALLGMLLHMTWHVVDGADG
                     DLARMTGRSSPIGEMVDGLCDYASHIVLYLVLGWLLSTSMNPPAWPTAHPLLWMWAAG
                     LSHIVQSNHVEVQRRQYQWWVYGTSWIRMNHSAESATGKSMFGGIVSVYLALATGLTP
                     HALKIDAAVADSESDPARREAIRAAVRAEAPPLLFLCKVLGPNPRAIVLGLSMLAGSP
                     LWYFLYQSLVLNALLVVSVIAHNRAAKRIVARIAP"
     gene            7872..9086
                     /locus_tag="VN627_06140"
     CDS             7872..9086
                     /locus_tag="VN627_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXD02871.1"
                     /translation="MADHLLNDAREIAPDIVALRRAIHAEPELGLITPKTRDKVRAAL
                     ADLPLEWREGPSTTGMVATLRGGAGAGGADRRRVLLRGDMDALPMPEETGLAFASTVP
                     GAMHACGHDAHTAMLVGAVRLLCARRDGLAGDVQFMFQPGEEGHHGARFMLDDGLIDP
                     LPHAAFALHVMPNAPHGLVAGREGALLAAADQISIAIEGRGGHASMPHDTLDPVPVAA
                     EIVMALQAMVTRRFSAHDPVVVTIARIEAGSAHNVIPDRAVLTGTMRTLSPGHRGELH
                     IAVARLAEHIAAAHGLRAEVTIREGFPVTICDGRAVDFGEGVARELFGAQAFHRLPDP
                     IMGAEDFAYVLEQVPGAMFFLGVAHEGDNWQECCGIHSTRMMVDESVLPQGAALLAGL
                     GERFLARGFGGG"
     gene            complement(9268..12552)
                     /locus_tag="VN627_06145"
     CDS             complement(9268..12552)
                     /locus_tag="VN627_06145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HXD02872.1"
                     /translation="MSGQTTNGAFFAEPVAASNFSFLAGASHPGEMVGRAHALGLAGL
                     GLADRNTVAGVVRAHKAWQEVGGAKGALRLIVGARLVFADGTPDVVVYPRTRHGWGRL
                     TRLLTLGNRRVEKGQCHLTLPDLLDHAQDMALIAMDGDADLLATLRETGQPLWRAARM
                     PRAGRDARRLAQAMALARESGVPLIATNDPLFAGPEDRPLHDVLTAIRLGQTVQTAGR
                     ALAANAERHLKPPAEMARLFARCPEALAATRDLLDAVRFTLPELAYEYPHEPVPEGWS
                     PQDWLEHLVREGGRRWFPAGLPEAYERTLAEELTLIAKCNYAFYFLTVHDIVRHARSL
                     DPPILCQGRGSAANSLVCYFLGITPIDPVKEKLLFSRFLSEQRNEPPDIDVDFEHERR
                     EEVIQYIYQRYGRERAGIAATVIHYRPRSAIREVGKALGFSEDITARVSGTIWGHWGG
                     SRLPPERLDEAGFDPDSPEMLRLRDLVGRILEFPRHLSQHVGGFILTEGRLDEIVPIH
                     NGAMEDRTFIEWDKDDIEELGLMKVDVLALGMLTCIRKAFDLLRGHGLGDYTLATVPR
                     EDRATYAMLQTGDSIGTFQVESRAQIAMLPRMKPKELYDLVIQVAIVRPGPIQGGMVH
                     PYLRRRNGEEKVEFPGPPDNPDELKEVLGKTLGVPLFQEQAMKLAIVAAGFTPGDADG
                     LRRAMATFRHMGTVGHYQDKLISGMVRRGYPQDFAERCFEQIKGFGEYGFPESHAQAF
                     GWLAYVSSWLKCHWPAAFTCALLNSQPMGFYAPAQLVRDARDHGVQVHCIDVNASEWN
                     STLEPSPLPAKGEVGGGVSRARDHALRLGLRQIDHFREAWAEALVAARQDGPFASLED
                     LARRAALPPRALSLLADADALGSLALTRREAGWDVRRVPPRQLSLFDAMAAPELAAEP
                     DAALPPMPLAEEVVADYQTQRLSLKGHPMQFLRARLARRGILSCAEVNAAKDGAKVRT
                     AGVVLIRQRPGKGNALFITLEDETGIVNALMWARDFEQQRRAVMAARLMVIDGEIQRS
                     KEGVVHLMVKTVHDATRLLGELAAGEHCEPELARGDAIKHPAASPNPGRHPRNVRILP
                     RSRDFH"
     gene            complement(12549..14084)
                     /locus_tag="VN627_06150"
     CDS             complement(12549..14084)
                     /locus_tag="VN627_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HXD02873.1"
                     /translation="MFLALWLPWLACEAARPAGQPDAPFALVVRQGSALRLAATDPRA
                     ETLGLAAGMTLADARARCPELVTTLEDPARTAAALERVLAALGRFTPVVAADPPDGAL
                     LDMTGCAHLFGGEAALLRILAEEMTRLDHAPRTAFAANAAAARALARHGAHDGSDDIR
                     ALPVAALEPPAETLVALRRAGLATLGDLAARPAPALAARFGEGLVLRLRQLLGEVAAP
                     LVPRGHAAPLRAEERFPEPVARTDDVLDVAEDLLRRLGRDMEERRLGGRRFVVTLCRA
                     DGARRRLAVETGRPTRDPAPVMRLLRERIESLADPLDPGFGYDSVALAITRSEPLSPH
                     QIGLEQGKEAVDDSIAALVDQLAVRLGPEAVYRLVPANRHLPEVAQAALPLAQGAPGR
                     AASFAARLGESPPRPLCLFDPPQPVEVIAGVPDGPPLRFRWRGHLFAVRLAEGPERIA
                     AEWWRHEGAHSGNLAGHPENGALTRDYYRVEDSEGRRWWLFRHGLHAEQAHPQWYLHG
                     QFP"
     gene            complement(14020..14799)
                     /locus_tag="VN627_06155"
     CDS             complement(14020..14799)
                     /locus_tag="VN627_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02874.1"
                     /translation="MPRPSISGESPSCPPDEAAGSGIPGLDPGRLHEVHAAGDDRAAA
                     LAFALVQAGAGGRPLFLVRPRGGGRRRAGLHGEGLAWLGLDAARLTLVEAADETVLLR
                     AALDAARCPALAAVVIESAGRLAAYDLTASRRLALAAERSGSRVVLVRIAAEPRPSAA
                     FTRWTIASAPSAPLAGAAPGRPVLAARLDRRRGWAGRQAHWLLEWIPERDSSHGFFRP
                     LPPASPETASPETASPWPAAPVPGAVAALAGLRGGAASRAA"
     gene            14917..>15436
                     /locus_tag="VN627_06160"
     CDS             14917..>15436
                     /locus_tag="VN627_06160"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXD02875.1"
                     /translation="MASQVQDLSPGETRSPPRSGRARGKREAIIAAAKQVFFQEGYAG
                     AAMDRITAEAGVSKATVYNHFRSKEELLLAVVDDVIVPFHAEYRAVLDHDAPFVEWLT
                     DLAVTVARKATMPETIALTRLLMAESLRFPKFGQTFQEALIDRTLELYRPKFEQAIAA
                     GILRPGNVYHIME"
BASE COUNT         2222 a         5217 c         5564 g         2433 t
ORIGIN      
        1 cggagaaggg gaacccgtcc acaatccggt tcttgtgctc acgcgcatgg ttaagaagta
       61 actgtcggtt tcgctgcaaa atggtggctt taccgagcat tagtattagg gcataggtta
      121 ctcgcttccc tgatgggatg atggagttcg tgcgatgaag aagactgaga agaccgtctg
      181 gactgctccc gccctgcgca agctcggcac gatcggcgat gtggccggca gcaacaatgg
      241 catcgttcag aaccaggcac cgggcaaccc gaagagctga tccgccgccg cggcgcctga
      301 cccttgattg ctgcctggtg cagctggagc ccggccggct ccggactgga cggtaatggg
      361 gcgatcgggg ccatggctcg gtcgctgggc ctgttcgcag gtgttccggc gaaggcgtgg
      421 agccgatcat tttcaaacga ttttggggct ggtccggagc gggccggccc tcttggcgtc
      481 tcggacgcgg gcagcggctc cttgcgcgcc ggagcggtgg ccggcctggg tctcgccttc
      541 ctcgacagtc cgcggcccgt gccgggtcaa cgggcccggg gtggcgggtg ggagccagaa
      601 ccctgccccg acggcagccg tgcggttttt catggctggt tcgacaatct ggcagaaatt
      661 gccacccacc tcggcctcgc ccccggcgcg agcgccgcgc gggtctatgg cgaggccgtg
      721 gcgcggtggg gcgatgccgc cgattcgcgg atcgtcggca cctatgccgc ggttgtagcc
      781 ggacccgacg gcctgaggct gtcacgcagc ccgtggtcag cgccggcgct gttctacgcc
      841 aacgacgatg cccgcgccat ggctgcctcg gccctgcgcg ctctggctgc cggcggcatg
      901 gagcgggtgc tcaaccgcgc cgccctggcc gccatggtca gtttctgcac cgagtgggat
      961 gatcagacct tctacgaagg cgcgcggatg gtgccgcacg gtgccgttgt gcatctccgg
     1021 ccgggcgcgg cgcgggtggt caactggtac gatccgcagg ccatcccgga ggttcggttc
     1081 tctcgcgacg aggactatgt ggccgccgcc cgcgagcagc tcgacgcagt catcgccgcg
     1141 accctgccct atgcccggcg gtccggcatc gcgctctccg gcggactcga ttctgcgatc
     1201 gtcgcggccc acgtgctgcg tcagctggcg ccggggcaga ccctgccgag ctttactttt
     1261 acgcctcatc cggactggga cggctccacc ccgcctggca cgttcggcga cgagcgcgcc
     1321 gcggtggagg ccttcgcggc gatgcacccg gcgctccgcc cgctttttcc ggatgatagc
     1381 gatgccggct acgacgaggc ggcgcgccag tttgcccagg cgatggacgg ctttcaccgt
     1441 cacctccatc tcgccgcacc ctaccagggc gtgtggcggg ccgcgcgcga ggcgggctgc
     1501 gacctcctgc tgacagctga ctggggcaat ggcaacattt cctccgatgc gccgtgggcc
     1561 ccggtggaat tcctgcgcac gggcgagtgg gagcaactgg tcgcgctgct gcggggcaag
     1621 cggggcgata cgcggccgct gtggcgccgc ctgctcgccc gcgccgtcct gccgcagctg
     1681 ccgcggccgt tgcgccgcgc cgcgcggcgg ttcgtccacg gcgatgcctc gcgagttagg
     1741 gcagacaccc gcctggcgcg gcccgaggtg atggagcgct acggcgtggg cccgtgggcc
     1801 gggacgcggc tgccgatcga ctatttcgag agcccgcgta cccgcgccga gaacgtgctc
     1861 tgccagtacg aatgggcccg ggtcgacagc ggcgaggcgc ttaccgccta tgaacaggtc
     1921 cacggcatcc gcggccgcga catcaccgcc taccgcccgt tgatcgagct gtgcctgggc
     1981 atgcccgcca gccagttcgc ccgcgacggc gtcgatcgct ggctggctcg gcgcatgggc
     2041 gcggggctga tgcccgaggc gcagcggacc aacaatctct acgggcgcca ccacgccgac
     2101 tggcacgtgc ggatgacccc ccggcgcgag cgcatgcgcg aggatgcgct gcgccttgcc
     2161 gagcacccgt ggctcggtga atggttcgat accgagcgca tgttgaagct gatcgacgat
     2221 tggccggccg agacgacgtt tgacgaagcc atcgaggtgc cgcgccgcta tgccctgccg
     2281 ctggtcctca atgcggggga tttcgtcaac tggttcgagg gccgcaatgc ctgaccgggg
     2341 cggcgcgacc aggggcggga ccagccaggc cgatccggcc cgcgccggtg cccacctgct
     2401 cgcccgggca gtgccccgcc cgctgctggc cggcgtgctg gcgctgggcc tggccgcgtc
     2461 gctgaccgag ggcgtgggcc tgatgctgct ggtgccggtg ctggggctcg tcggcgccgg
     2521 cgggaatgcc ttgcccggga gcgcccccgg gcggctggcg gcgctgtggc ggcacctggg
     2581 cggggcgcta ccgggggata ttgccgggct gcttggtctg tttgtgctgg tcgtcgcctt
     2641 gcgggccgcc cttgtgcagg ggcgcatcct tgccgaacag gcactgcgat tggcagtgac
     2701 caataacctt cggcgcgacc tgttcggcgc cctcctgcac gccgattggc tatggctcgc
     2761 cggcgcacgg caggggcgcc tgctgggcgt cgtgctgggc acgatagatc ggctcggcgc
     2821 cggcctgcag cacctgcagt cgctgttcgc cgcgctggtc accctcgcca tcatgctgat
     2881 tgcggcgctg ctgatcgatc cccttccggc cgcagcgctg gggcttggcg ggctgctggt
     2941 gctggccatc cacgtcgccc tgcgccgctc cgcccggcgc gacggcgacc gggtgaaccg
     3001 gggctatgag gactactacg gcttctatgc cgagcgcctg gccatgctgc ggcagatcaa
     3061 gagcttcggg gccgagcggc gcgaggcgga ccgcgccggg gtggtggccg cggcgctgcg
     3121 ccgggcacag ctgggtttca gccgggggct tggcctcggc aacttcgtcc tgcaggcggg
     3181 cacggcggct gtgctgggcc tcgctgtatg gctggcagtg acgaggtggc attcctcccc
     3241 cgtcaccctg ctgccgctca tcgccatttc cgcgcgcgcc gtgcccctgc tcagcgtggt
     3301 ccagtccggg ttgcagaacc ttgcgcatga tcggcccgcc ctggccgaac tgggcgcctg
     3361 ccagctcgag gccctgcact atgccgagag tgaaagcgcc agcgaaagcg ccggcgtgcc
     3421 gctgccgccg gcccggcgcg agctgcgcct ggaagcggtg agcctgtacc atgccgggcg
     3481 ggagcaggca gcactggacc ggctgacgct gacgctggcg gcggggagca tggtccacct
     3541 tgcgggcgaa tcgggcgcgg ggaagtccac ccttgccgac gtgctcgccg ggctggtctc
     3601 gcccacctcg gggcgcctgc tgatcgacgg aatggcgatc ggtccagtcc agcggcgggg
     3661 ctggcggcag cgggtggcct atgtccagca ggagccggtg ctgcttcatg cgtcgatccg
     3721 cgacaacctg cgctgggctg ccccgggggc aagcgacgcg caggtggagc aggcgctgcg
     3781 cgaggcggcc gcacagttcg tcttcgccct gccggacggg ctcgacaccg tggtgggtga
     3841 gcgcggcgcc agcctttcgg gcggcgagcg ccagcggatc gcgctagcgc gcgggctcct
     3901 gcgcgaaccc gatctcctga tcctcgacga ggtgacctcg gcgctcgatg ccgccaacga
     3961 agcggccgtt gcgggggcga tagcccggct gcgcggccgg atgacgatcc tgatcatcgg
     4021 ccatcgcgga gcgcttgccg gcctggccga gcggcggctg gtcatggccc agggccggct
     4081 gacggcagac gaggcgagcg gcaacgaggc cgccagggct tgatcccggg ccacatgttg
     4141 cgcatggagg gcattggaat agggacgtca gaacaaaccg gatgattgca tgatctcgtc
     4201 ccgtattgcc gcttgccttg cccttgccgc cgtggccttg ctgccgcttc gggccgccgc
     4261 cgcccctgcc gcggcgatga ccggggtgtc tccaccggcg cccgccggct gtaccggcag
     4321 gccgagcgcg acctggatcg acgtatcggt ggaaggcctg cgctcgggct cggggcaggt
     4381 ggcgatcacg ctctatgccg ataattcgcg caagttcctg gtgaagaacg gttcgctcta
     4441 cgtgggccgg gtgcccgccc aggcagggac gaccaccggc tgcatctttg tgccccagcc
     4501 cggcgtctac gccatcgcta tctaccacga tgagaatggc gacggctctt tcaaccggac
     4561 ggcgatcggc ttcccggccg agggcttcgg cttcgccaac aacccgagca ccctggccgg
     4621 cttgccgagc ttcaagtcgg tgcgcctcgc catcccgcgc gccgggctgc tgacgcgggt
     4681 gcggctgcgc tatccctgaa aaagcggtca gcctgaaacg aggttgagct gcgcgggtgc
     4741 ctcggcctcg gcgatggcat gggcgcgcgg atcgtcgacg tcgagccacc aggccccggc
     4801 gatctccatg gtcgcggcgc ggccccggtc ggcgaggacc tgcatcccgt ccgaaaggct
     4861 gccgcccttg ccggcggcga tggcggcggc aatcgcctcg gggagttccg gagtgacgag
     4921 gaaagcgccg cagtcgacgg cgtcgtaaac gggaatggtc ttgccgatgg ccacgatgcg
     4981 gccgtccggc ccggtctgca cccaggtggc atcgtcggga tcgaccagcg gattgtccac
     5041 ccggcgatcg acggcgaggg tcaccccgcg ctccggcgat ccttcggcgg cgaggcgccg
     5101 gaggatctcg gccgagaaca tgtgatcggc catgaccagc aggaagtcgc cgccgcccag
     5161 ggcagcgacc ctgtccgccc cggcgatcac cgagaagccg ttgggcttgg accagtcgct
     5221 cacccgttcg gcatggatgg ccatgccggt gcggcgggcc agggatgcga ggaaggcctc
     5281 aacccgctca ccctcgtgcc ccgtgacaac cacgacgcgg gtaaccccgg cggcggcgag
     5341 ctggcggatg ccgagctcga tcaggggaat gccggcgacc ggggtgagcg gcttggagtc
     5401 cgacaatgcg ctgatgcgac tgccgaaccc ggcggcgatg atgaccgcgt ccaagctgct
     5461 gcccccggtt gcccggcgct tattcggcgg cgatggcggc cggggcgata tattcctcga
     5521 ccatccgcgc ggcgtcgtca tcgttgaact ggactggcgg atgcttgcag aaataagccg
     5581 acggaccggc cagcgccccg ccttcgccac gctccagcgc gaccttgcag caacggatcg
     5641 cgtccatcac gcaggcggcc gaattcgggc tgtcttccac cgagagacgc agctcgaggt
     5701 tcatggggac gttgccccac tggttgccct caagccgcag gaagcacagc ttgttgtcct
     5761 tctgccacgg cacgtagtcc gacgggccga cgtggatgtt ctcgtcctcg aggcgctggg
     5821 cgagcatggc ctggacggct tccgtcttcg attccttctt ggagccgaga cgctggcggt
     5881 ccagcatgtt catgaagtcg gtgttgccgc cggtgttgag ctggtaggtc cgctcaacat
     5941 tgacgccgcg cgcgccgaag aggctggcga ggacgcggtg gacgatcgtg gcgccgacct
     6001 gggccttgat atcgtcaccc acgatcggaa tgcgcttctc ggcgaagcgg cgttcccatt
     6061 cggggcggct ggcgatgaac accggcatgc agttgaccac ggcaatgccc gcttccagcg
     6121 cgcattccat gtagaactcg gtcgcctctt ccgagcccac cggcaggaag ttgacgagga
     6181 tctcggcgcc cgaggccttg agcgcggcga tgatcgattc ccgggtggct tccggctcgt
     6241 cggcgacgat gaagccgcgc tcgcccatgc cggtcatgtg gtcggccacg ccatcgagga
     6301 tcttgcccat gatgaccttg ccgccggtgg cggggacatc gggctggaac acggcggtgc
     6361 agttcgggcc ggcgaagatc gcctcggcca cgtccttgcc gaccttgcgc ttatcgacat
     6421 cgatgccgag cacgaaatcg acgtcgcccg cgccgtagcc gccgatcacc tcgtggatca
     6481 ggccctgcgc ggagttgttc tggcggtagt aggcaacccc ctgcaccagc gagctggcgc
     6541 aattgccaac gccgatgatt gcaaccttga ttggcttcat caaaacggat cccttcaggc
     6601 gaaggctcct cgacgccgca gcgaccgggc cttcgtgatt catgtttggt tgagcaacct
     6661 atcgcttccg ctgatgccct tcgccacgag tggcaatgac aaacttgcgg aaaacaacga
     6721 ttttcttcgg cccagcgttc cccgactagt gtcatcggcc acgtcagcag ccaaaggcgg
     6781 ttggaaaagt catgctcggg gtgcgtaact atagttggcg cgccgtggcc gcaagcgatt
     6841 atgacgattc ggtgaaggcg gtttgccgca ggaatgcgag gaggggatgc gttgagcgac
     6901 ttggccaccg gtgccggagg gcagcagcag gcggaggcgc ggcctcggcc gcagcgcccg
     6961 cgcgagctgc aggacgcgct caactactat ctctaccatc ctctcgcctg gcagctggcg
     7021 cgcctgctcg cgcgcacgcc gctgacgccc aacatggtct cggtgatcgg cgggagctgc
     7081 gtcgtcgcgg ccgcggtcgt ctatgcgcag cccgggtggg cgcagtttcc gtcaggcccg
     7141 gcgctgctcg gcatgctgct gcacatgacc tggcacgtgg tcgacggcgc cgatggcgac
     7201 ttggcccgga tgacgggccg gtcaagcccg atcggcgaga tggtcgatgg cctgtgcgac
     7261 tatgccagcc acattgttct ctacctcgtg ctcggctggc tgctgtccac cagcatgaac
     7321 ccgcccgcgt ggcccacggc ccatcccctg ctgtggatgt gggcggcggg cctcagccac
     7381 atcgtccagt ccaaccacgt ggaagtgcag cgccgccagt accagtggtg ggtctacggg
     7441 acgagctgga tccggatgaa ccacagcgcc gaatcggcga cgggcaagag catgttcggc
     7501 gggatcgtct cggtctatct cgcgctcgcc acggggctca cgccgcatgc gctgaaaatt
     7561 gatgccgccg ttgcggacag cgaaagcgat ccggcgcggc gcgaggcgat ccgcgcggct
     7621 gtccgggccg aggccccgcc gctgctgttc ctgtgcaagg tgctgggccc gaacccccgc
     7681 gccatcgtgc tgggcctctc catgctcgcc ggcagcccgc tctggtattt cctgtaccag
     7741 tcgctggtgc tcaatgcctt gctggtggtg tccgtcatcg cccacaaccg cgccgccaag
     7801 cggatcgtgg ctagaatcgc cccgtaatcc gcctgactcg acttcgggag ggcgcgcagt
     7861 atcatcgtgc catggccgat cacctgctga acgacgcgcg cgaaatcgcg ccggacatcg
     7921 ttgccctgcg ccgggcgatc catgccgagc cggagctggg cctgatcacg ccgaagacgc
     7981 gcgacaaggt tcgcgccgcg ctggccgacc tgccgctgga gtggcgcgag gggccgtcga
     8041 ccaccggcat ggttgccacc ctgcgcggcg gggccggagc cgggggagcc gaccgccgtc
     8101 gggtcctgct gcgcggcgac atggatgccc tgccgatgcc ggaggaaacc ggcctcgcct
     8161 ttgcctccac cgtgccgggc gccatgcacg cctgcggcca cgatgcccac accgccatgc
     8221 tcgtcggcgc tgtccgcctg ttgtgcgcgc gccgcgacgg gcttgccggc gacgtgcagt
     8281 tcatgttcca gccgggtgag gaaggccacc acggcgcgcg gttcatgctg gatgacggcc
     8341 tgatcgatcc tctcccccac gcagctttcg ccctccatgt catgcccaat gcgccgcacg
     8401 gtctcgtcgc cgggcgcgag ggggcgctgc tggcggcggc cgaccagatt tccatcgcga
     8461 tcgagggacg cggcggccat gcgtcgatgc cgcacgatac gctcgatccc gtgccggtcg
     8521 ccgccgagat tgtcatggcg ctccaggcca tggtcacccg gcgcttttcc gcccacgatc
     8581 cggtggtcgt caccattgcc aggatcgagg caggcagcgc ccacaatgtc atccccgacc
     8641 gcgcggtgct gaccggaacc atgcgcacgc tgagccccgg ccaccgcggc gagctgcaca
     8701 tcgcggtggc ccgccttgct gaacatatcg ccgccgccca cggactgcgg gccgaagtga
     8761 ccatccgcga gggctttccg gtgacgatct gcgatggccg cgcggtcgat ttcggtgaag
     8821 gcgtggcgcg cgagctgttt ggcgcccagg cctttcaccg cctgcccgat ccgatcatgg
     8881 gcgcggaaga cttcgcctat gtgctggagc aggtgcccgg cgcgatgttc ttcctcggcg
     8941 tggcccacga aggcgacaac tggcaggaat gctgcggcat tcactccacg cgcatgatgg
     9001 tggacgaaag cgtgctgccg caaggcgcgg ccctgctggc aggcttgggc gagcggttcc
     9061 tggcgagggg gtttggaggg ggctgagact tccccctgct ccgttcgtcc tgaggaggcc
     9121 gaagccgcag gcagaagccc cgtctcgaag gaccttgccc tgggaccgct gggtgcttcg
     9181 atatggcctc tgcctgcggc ttcggcctcc tcagcacgaa cggagggtga ggcggggcat
     9241 ttccgcagcc cgctcggggg tgcgggttca gtgaaaatcg cgtgaccttg gcaagatccg
     9301 cacgttccgc gggtgccgac ccgggttagg gcttgccgcc gggtgcttga tggcatcgcc
     9361 gcgggccagt tccggctcgc aatgctcgcc cgcggccagt tcgcccagca ggcgagtcgc
     9421 atcgtgcacc gtcttcacca tcaggtggac cacgccttcc ttgctgcgct ggatctcgcc
     9481 gtcgatcacc atcagccggg cggccatgac cgcgcggcgc tgctgttcga aatcgcgggc
     9541 ccacatcagc gcgttgacga tcccggtctc gtcttccagc gtgatgaaca gggcattgcc
     9601 cttgcccggg cgctggcgga tcaggacgac cccggcggtg cggaccttgg cgccgtcctt
     9661 cgccgcgttc acctcggcgc aggacaggat gccccgccgc gccagccggg cgcggaggaa
     9721 ctgcatcggg tgccccttca gcgacaggcg ctgggtctgg taatcggcca cgacctcctc
     9781 ggccagcggc atcggcggga gcgcggcgtc gggctcggcg gcgagctcgg gcgcggccat
     9841 ggcatcgaac agcgagagct ggcgcggcgg cacccggcgc acgtcccagc cggcttcgcg
     9901 gcgggtgagg gcgagcgagc cgagcgcatc ggcatcggcc agcaggctga gcgcgcgcgg
     9961 cggcagggcg gcgcggcggg cgaggtcctc caggctggcg aacgggccgt cctgccgggc
    10021 cgcgaccagc gcctcggccc aggcctcgcg gaaatgatcg atctgccgca ggccgagacg
    10081 cagggcgtga tcacgggcgc gggacacccc tcccccaacc tctccctttg cggggagggg
    10141 agatggctcg agggtggagt tccattcact tgcgttcacg tcgatgcagt gcacttgcac
    10201 cccgtggtct cgggcgtcgc gcaccagttg cgccggggca tagaagccca tcggctggct
    10261 gttgagcagc gcgcaggtga aggcggcggg ccagtggcat ttcagccatg aggaaacata
    10321 ggccagccag ccgaaggcct gggcgtggct ttcgggaaag ccatattcgc cgaagccctt
    10381 gatctgttcg aagcagcgct cggcaaagtc ctgcggatag ccgcggcgga ccatgccgct
    10441 gatcagcttg tcctgataat gccccaccgt acccatgtgg cggaaggtag ccatggcgcg
    10501 gcgcagcccg tcggcatcgc cgggcgtgaa cccggcggcg acgatggcga gcttcatcgc
    10561 ctgttcctgg aacagcggca cgcccagcgt cttgccgaga acctccttca attcatccgg
    10621 attgtccggc gggccgggaa attcgacctt ctcctcgccg ttgcggcgtc tgaggtaagg
    10681 gtggaccatg cccccctgga tcggcccggg gcgaacgatc gccacttgga tgacgaggtc
    10741 gtagagctcc ttcggcttca tccgcggcag catggcgatc tgggcgcggc tttcgacctg
    10801 gaaggtgccg atggaatcgc cggtctggag catggcatag gtcgcccggt cctcgcgcgg
    10861 gacggtggcg agcgtgtagt cgcccaggcc atggccgcgc agcaggtcga aggccttgcg
    10921 gatgcaggtg agcatgccga gggcgaggac atcgaccttc atcaggccca gctcctcgat
    10981 gtcgtccttg tcccactcga tgaaggttct gtcctccatc gcgccgttgt ggatcggcac
    11041 gatttcgtcg agccggccct cggtcaggat gaagccgccg acgtgctgcg agaggtggcg
    11101 ggggaattcg aggatccggc cgaccagatc gcgcaggcgc agcatttccg ggctgtcggg
    11161 atcgaagcca gcctcgtcga gccgctcggg cggcaggcgg ctgccgcccc agtgacccca
    11221 gatcgtgccg gatacgcgcg cggtgatgtc ctcgctgaag ccgagcgcct tgcccacctc
    11281 gcgaatggcc gagcgcgggc gatagtggat cacggtggcg gcgatcccgg cccgttcgcg
    11341 gccatagcgc tggtagatgt actggatcac ctcctcgcgc cgctcatgct cgaaatccac
    11401 gtcgatatcg ggcggctcgt tccgttgctc ggagaggaaa cgggagaaca gcagcttttc
    11461 cttcaccgga tcgatcgggg tgatgccgag gaagtagcag accagcgaat tggccgccga
    11521 gccgcgcccc tggcacagga tcggcggatc gaggctgcgg gcgtggcgga cgatgtcgtg
    11581 gacggtgagg aagtagaacg cgtagttgca cttggcgatg agggtgagct cctcggcaag
    11641 ggtgcgctcg taggcctccg gcaggcctgc gggaaaccac ctgcggcctc cttcgcgcac
    11701 gaggtgttcg agccagtcct gcgggctcca cccttccggc actggctcgt gcgggtattc
    11761 gtaggcgagt tcgggcaggg tgaagcgcac cgcatccagc aggtcgcgcg tggcggcgag
    11821 cgcctcgggg cagcgggcga agaggcgggc catctcggcg ggaggcttca ggtgccgctc
    11881 ggcattggcg gccagcgctc ggccggcggt ctggacggtc tggccgaggc ggattgcggt
    11941 gagcacgtcg tgcagggggc ggtcctcggg cccggcgaag agcggatcgt tggtggcgat
    12001 gagcgggacg ccgctctccc gggcgagggc catggcctgg gcaaggcggc gggcatcgcg
    12061 tccggcgcga ggcatgcggg cggcgcgcca cagcggctgg ccggtttccc gcaaggtggc
    12121 gagcaagtcc gcgtcgccgt ccatggcgat cagggccatg tcctgcgcat ggtcgagcag
    12181 gtcgggcagg gtgaggtggc actggccctt ttccacccgg cggttgccga gggtgagcag
    12241 gcgggtcagc cggccccagc cgtggcgggt gcgcggatag acgaccacgt cgggcgtgcc
    12301 gtcggcaaag acgaggcggg cgccgacgat caggcgcaag gcccccttcg ccccgcccac
    12361 ctcctgccat gccttgtgag cacggaccac gccggccacg gtgttgcgat cggccaggcc
    12421 cagccctgcc aggccaaggg catgggcgcg gccgaccatt tcgcccggat ggctggcacc
    12481 ggccaggaag ctgaaattgc tggcggcgac cggttcggcg aagaacgccc catttgtcgt
    12541 ctggccgctc atggaaactg gccatggaga taccactgcg ggtgggcctg ctcggcgtgg
    12601 agcccgtggc ggaacagcca ccagcggcgg ccctcgctgt cttccacccg gtagtagtcg
    12661 cgggtcagcg cgccattttc ggggtggccg gccaagttcc ccgaatgggc accctcatgc
    12721 cgccaccatt ccgccgcgat ccgctccggc ccttcggcga ggcgcacggc gaagaggtgg
    12781 ccgcgccagc gaaagcgcag cggcggcccg tcaggcacgc cggcgatcac ctcgaccggc
    12841 tggggcggat cgaacaggca gagcgggcgc ggcgggctct cgccaaggcg cgcggcgaaa
    12901 gaggcggccc tccccggcgc gccttgcgcc agcggcaggg ccgcctgggc cacttcgggc
    12961 aggtggcggt tcgccgggac cagccggtag accgcttccg gcccgagccg taccgccagc
    13021 tggtcgacca gcgcggcgat gctgtcatcc acggcttcct tgccttgctc cagtccgatc
    13081 tggtgggggc tgagcggttc gctgcgggtg atggcgaggg ccacgctgtc gtatccgaat
    13141 ccggggtcga gcgggtcggc gaggctctcg atccgctcgc gcagcaggcg catgaccggg
    13201 gcgggatcgc gggtgggccg tcccgtttcg accgccagcc gccgccgcgc gccatcggcg
    13261 cggcacaagg tgacgacgaa gcggcggccg ccgagacggc gttcttccat gtcccggccc
    13321 agccgccgga gcagatcctc cgccacgtcg aggacgtcgt cggttcgcgc cacgggctcg
    13381 ggaaagcgtt cctcggcgcg caggggcgcg gcatggccgc gcggcacgag cggggcggcc
    13441 acttcgccca gcagctggcg caggcgcagg accagccctt cgccaaagcg cgcggcgaga
    13501 gcgggcgcgg ggcgggcggc gagatcgccc aaggtggcaa gcccggcccg gcgcagggca
    13561 accagcgttt ccgccggcgg ttcgagcgcg gccaccggca gggcgcggat gtcgtcgctg
    13621 ccatcgtggg cgccatggcg agccagcgcc cgggcggcgg cggcattggc ggcgaaggcg
    13681 gtgcgcgggg cgtggtcgag gcgggtcatc tcctcggcaa ggatgcgcag cagcgctgcc
    13741 tcgccgccga acagatgggc gcagccggtc atgtcgagca gggcgccatc gggcggatcg
    13801 gcggcgacca cgggggtgaa ccgaccgagc gcggccagca cccgctctag cgcggcggcg
    13861 gtgcgggccg ggtcttcgag cgtggtgacg agctcggggc agcgggcgcg ggcatcggcg
    13921 agggtcatgc cggcggccag gcccagcgtc tcggcccggg gatcggtggc ggcaaggcgc
    13981 agggcactgc cctgccggac gacgagggcg aaaggcgcgt caggctgccc ggctggccgc
    14041 gccgcctcgc aggccagcca gggcagccac agcgccagga acaggggccg cgggccaggg
    14101 acttgcggtt tccgggctgg cggtttcggg agaggctggc gggagggggc ggaagaaacc
    14161 atggctgctg tctcgctcag ggatccattc gaggagccag tgcgcctgcc ggcccgccca
    14221 cccgcggcgg cggtcgagcc gggcggcgag gaccgggcgg cccggcgctg cgccggcgag
    14281 cggggcggag ggggcgctgg cgatcgtcca gcgggtgaag gcggcgctcg gccgcggctc
    14341 ggccgcgatc cgcaccagca ccacgcggct gcccgaacgc tccgccgcaa gggccaggcg
    14401 tcggctggcg gtgagatcgt aggcggcgag gcgcccggcg ctctcgatca ccacggcggc
    14461 cagcgccggg cagcgcgccg catcgagcgc agcgcgcagc aggacagtct cgtcggcggc
    14521 ctcgaccagg gtcagccggg cggcatcgag ccccagccag gccagcccct cgccatggag
    14581 cccggcccgc cgccgccccc cgcccctcgg gcggacgaga aacagcggac gcccgcccgc
    14641 ccccgcctgg accagcgcga aggcgagcgc cgccgcccgg tcatccccgg cggcgtggac
    14701 ttcgtggagc cggccgggat ccagccccgg aatcccgctg ccggcagcct cgtcgggcgg
    14761 gcaagagggg ctttccccgg aaatggaagg acgtggcatc tgcgaatcac ccgataatgt
    14821 tcttattatg ttccattaag cgaggatgtc caccatggcc ggggagaaac gcgtcttaat
    14881 ctggcaatta ccgctttcgg tcattaggct ctccccatgg ccagccaagt ccaagacctg
    14941 tctcctggag aaacgcgttc gccgccgcgc agcggccgtg cgcgaggcaa acgcgaggcg
    15001 atcattgccg cggccaagca ggtgtttttc caggaaggtt atgccggggc cgcgatggac
    15061 cggatcaccg ccgaggccgg cgtgtcgaag gccacggtct acaatcactt ccgctccaag
    15121 gaggaacttc tcctcgccgt ggtggatgac gtgatcgtgc cgttccatgc cgaataccgg
    15181 gccgtgctcg atcacgacgc ccccttcgtc gaatggctga ccgatcttgc cgtcaccgtg
    15241 gcgcgaaagg cgaccatgcc ggaaacgatc gcgctcaccc ggctgttgat ggcggaatcg
    15301 ctgcgcttcc ccaaatttgg ccagaccttc caggaagctc tgatcgaccg cacgctagaa
    15361 ctctaccggc cgaagttcga acaggcgatc gccgcgggca tcctgcgccc gggcaatgtt
    15421 taccacatca tggagc
//
LOCUS       DATNBV010000256         2945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_454823,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000256 DATNBV010000000
VERSION     DATNBV010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454823"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VN627_06165"
     CDS             complement(<1..376)
                     /locus_tag="VN627_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02876.1"
                     /translation="MLRLSDLALPLDHAPADLEAAIIARLGLGPGQLRRFAVARRGND
                     ARNKRAIKLVYVLDLDLADEAAVLAAHADDPHLRPTPDTTYRPPVRAPEGWSGERPVV
                     VGAGPCGLMAALILAEMGLKPIV"
     gene            complement(386..1624)
                     /locus_tag="VN627_06170"
     CDS             complement(386..1624)
                     /locus_tag="VN627_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02877.1"
                     /translation="MQVATAIAGLLLARLMPVHSYAVYTVAMTVIGAIWVITKSGIQT
                     AFAAELGRVWPSREAAAVVLASALNARRRISLWTMPLILAASAWLLWRAGAQWWELGG
                     ATAIVFLIWAADLRGGLYDLLLYFDERTGQVRAVDTGLAIARMAIIGLLALAKTVYLV
                     PALLTSLLLTAGRVPPLQRWSRQVLGRPLPPSDPAVEATITRVARAQIPADIFFCLQS
                     QIVMFHLTGGDPLQLATYGALGRIAQLLAPFSGLGLAFCVPAFAAVTDRALLRIGFMV
                     AACSLPALALVAGAGLAPELLLAVIGPNYASQHGALFTSALSAALVFAVQATWDLVAH
                     RGWNEWASVRIPLGIAWALAAPLLLPVDSAAGAYLFYAGFSLCTLVAAALGLTSAMRR
                     GEIALLGRRETPLPKPAIPG"
     gene            complement(2091..>2945)
                     /locus_tag="VN627_06175"
     CDS             complement(2091..>2945)
                     /locus_tag="VN627_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HXD02878.1"
                     /translation="HLINRRAAQMEAEGVSFRTSTEVGVNISVDSLKENFDAIVLSGG
                     AEDPRKLEIPGAELLSVRPAMQFLMQQNKRNAGDDELRAAPQGSLTATGKHVIVIGGG
                     DTGSDCVGTSNRQGAASVVQLEIMPKPPEKEDKALTWPNWPLKLRTSSSHEEGCERDF
                     AVLTKRVVGDNDVSGLECVRVEWVDGRMQEIPGSEFTLKADLILLAMGFVGPRKQGLL
                     DQAGVTLDARGNVAANVVDYTTSDPQIFACGDMRRGQSLVVWAIREGRQAARAVDLAL
                     MGASELPR"
BASE COUNT          509 a          955 c         1066 g          415 t
ORIGIN      
        1 caacgatggg cttgaggccc atctccgcga ggatcagggc ggccatgagc ccgcagggtc
       61 cggcgccgac gacgacgggg cgttcgccgc tccagccttc cggcgcgcgg acgggagggc
      121 ggtaagtcgt atccggcgtg gggcggaggt gcggatcgtc ggcgtgggcg gcgaggacgg
      181 cggcctcgtc ggcgaggtcc aggtcaagca cgtagaccag cttgattgcc cgcttgttgc
      241 gcgcatcatt gccgcggcgg gcgacggcga agcggcgcag ctggcccggg ccaagaccga
      301 ggcgggcgat aatggccgcc tcgaggtccg cgggcgcgtg gtcgagcggg agggcgaggt
      361 cggacaggcg gagcatcggc tggccctagc ccggaatggc cggcttaggc aaaggggtct
      421 cacgccggcc gagcagcgca atttcgcccc ggcgcatggc cgaggtcagc cccagggccg
      481 ccgccaccaa agtgcacagc gagaagccgg cgtagaacag gtaggccccg gccgcgctgt
      541 cgaccggcag cagcaggggg gccgccagtg cccaggcgat cccgagcggg atacgcaccg
      601 aggcccattc attccacccg cggtgcgcga ccagatccca agtggcctga accgcgaaga
      661 ccagagccgc gctcaaggcg ctggtgaaca gggccccgtg ctggctcgca tagttggggc
      721 cgatgacggc gagcagcagt tcaggcgcca gaccggcccc ggcgacgagg gccagggccg
      781 gcagcgagca ggcggcgacc atgaacccga tgcgcagcag cgcccggtcg gtcacggcgg
      841 cgaatgcggg cacgcaaaag gccaggccaa gcccgctgaa cggggcgagc agctgcgcga
      901 tgcggcccag ggcgccgtag gtggccagct gcagtggatc gcctccggtg aggtggaaca
      961 tgacgatctg cgactggagg cagaagaaga tatcggccgg gatctgcgcg cgggcgacgc
     1021 gggtaatggt cgcctcgaca gcggggtcgc tgggcggaag tggccggccg agcacttggc
     1081 gggaccagcg ctgaagcggc ggaacccgcc cggcggtgag cagtaggctg gtcagcaggg
     1141 cagggactag gtagaccgtc ttggctaggg cgagcaggcc gatgatcgcc atccgcgcga
     1201 tggcaaggcc ggtgtccacg gcgcgaacct ggccggtccg ctcgtcgaaa tagagcagga
     1261 ggtcatagag gccgccgcgc aggtccgccg cccagatcag gaacacgatc gccgtggcac
     1321 cccccagctc ccaccattgc gcgcccgccc gccacaataa ccaggccgag gcggcgagga
     1381 tgaggggcat cgtccacaat gagatccgcc gccgagcgtt gagcgccgac gccaagacca
     1441 cggccgctgc ctcgcgcgat ggccagacgc ggccgagttc ggcggcgaag gcggtctgga
     1501 tgccgctttt ggtaatcacc cagatcgccc cgatcacggt catcgccaca gtgtagacgg
     1561 cataagagtg gacgggcatc aggcgagcca gcaacagccc ggcaatggca gtcgccacct
     1621 gcatggtcac gccgcctgca atgaacgagc tgatcctgcg aaatgcggcc attgtcaggg
     1681 gctaggtcag tagcagtcga acatcaaccg ggcattgttg ctgcatcagt cgtgaggaaa
     1741 atcctgcgcg ggcgcatgca tcagccgttc aggccgacag ccatcgtcct tctgccgcca
     1801 ctcagttgga caggagagat gccgatcagt gtgccgagcc gatccgacgg agtgacatca
     1861 aggcagggtc atgccaagat cggggacgct cagcgacgga gcggtgcttg tcacatgcgg
     1921 gtcgcccggc tgattttcgc ggctcctcgt gacgaggcgc accccaaacc gcctggccgg
     1981 ccgctcaact gcaaaggata gcgcagcggc gagggggatt gccgtgatgc gcaggaacgg
     2041 acggccagat cggcacgtcc gttcctgttg ggttgccgcg atgggccccc tcagcgcggc
     2101 agttcgcttg cccccatcag cgccagatcc actgcgcggg ccgcctggcg gccctcgcgg
     2161 atcgcccaga ccacgaggct ctggccgcgc cgcatgtcgc cgcaggcgaa gatctgcgga
     2221 tcgctggtgg tgtaatcgac cacgttggcc gcgacattgc cgcgggcgtc aagggtgacc
     2281 ccggcctggt cgagcaggcc ctgcttgcgc gggccgacga agcccatggc gagcaggatc
     2341 aggtcggcct tgagggtgaa ctcgctgccg gggatctcct gcatgcggcc atcgacccat
     2401 tcgacccgga cgcattcgag gccggagacg tcattgtcgc cgacgacgcg cttggtcagg
     2461 accgcgaaat cgcgctcgca gccttcctcg tggcttgacg aggtgcgcag cttgagcggc
     2521 cagttcggcc aggtcagcgc cttgtcttcc ttttccggcg gcttgggcat gatttcgagc
     2581 tggaccaccg aggcggcccc ctggcggttc gacgtgccga cgcagtccga accggtgtcg
     2641 ccgccaccga tcacgatgac gtgcttgccg gtggcggtga gcgaaccctg cggcgcggcg
     2701 cggagctcgt cgtcacccgc attgcgcttg ttctgctgca tcaggaactg catcgccgga
     2761 cggaccgaga gcagctcggc gcccgggatc tccagcttgc gcggatcttc cgcgccgccg
     2821 gagaggacga tggcgtcgaa gttttccttc agcgaatcga ccgagatgtt cacgcccacc
     2881 tccgtcgagg tgcggaagga gacgccttcg gcctccatct gcgccgcgcg gcgattgatg
     2941 aggtg
//
LOCUS       DATNBV010000257         2762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1430051,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000257 DATNBV010000000
VERSION     DATNBV010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430051"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1461
                     /locus_tag="VN627_06180"
     CDS             205..1461
                     /locus_tag="VN627_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HXD02879.1"
                     /translation="MSHPSSLSGAFRKPFPQQIAFFRNKLKNLVPTQRWDDLTGEAHD
                     TAFMVAGAAKADLLTDLAAATDKAIAEGRGIEEFRRDFRDIVKRNGWAGWTGEGSIKG
                     EAWRVKTILRTNAYTSYSAGRHAQLLDGEFAFWVYRHGDAREPRLVHLSFNGLALPPG
                     HPFWGKYYPPSDWGCSCYVVGARTAAGVRRLGGDPGKALPANWAALDPRTGEPKGIGK
                     GWGYAPGASVAPVVQALAGKIGSWDYRIAKAFMEGVPEAQRDALAQAYRSLPSTADDV
                     RRYAQRVYEPKPDLAELPPQRTLGLIPGDQAATIGKLIDREIAGYDFSIDQSGVGHVI
                     GEHGPGNETDKKQRAVDSADFARLPMVLQSPGSVTLSDKLSDMGETVIDYTKAIDGEE
                     WHVRMAVRGKKRRTLALKTMFIVIGK"
     gene            1543..2049
                     /locus_tag="VN627_06185"
     CDS             1543..2049
                     /locus_tag="VN627_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HXD02880.1"
                     /translation="MFNVQFNAGESREAIRRAIAALEDMTPLFQDVAEYMTEATRQRF
                     VTGTAPDGSKWPAKSQVTLDRYKRLGYGNLSRVLIGPGKRLSREIVSEATKGGAVIGS
                     ALIYSSVMQDGAPRGAFGTNSRGRPIPWGAIPARTWLGISSTDADQIVAIAEEHIAAS
                     VDGAEKGR"
     gene            2190..>2762
                     /locus_tag="VN627_06190"
     CDS             2190..>2762
                     /locus_tag="VN627_06190"
                     /inference="COORDINATES: protein motif:HMM:NF021627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HXD02881.1"
                     /translation="MTRTASQIALCSAVAFAAAEDVPEWVHLLPAGEIRTVDGRGPYR
                     VKSLQNVMARSLQGGAKLPIDECHVTDKGAALGLAAPARGWIVELQAREDGVWGRVEW
                     NSSGRQLMADKAYIGLSPVILHSRGGDVMQLLRASLTNTPNLQGLTTLHSESNTMDWK
                     AKLIELLGLDSNADDAAIEAALTAKMSGGDK"
BASE COUNT          498 a          870 c          990 g          404 t
ORIGIN      
        1 atgagcatgc ctcgatttgc ctccagaagg ccactgagcg aggggagcgc cacgcttcgc
       61 cgcagaacgt cgccgacccc cttctcaggc cttctcaaac ccgttttcgg ggcaatcggt
      121 gcccggctcg actgtgcgat ctgcgcgatg ggaaggacct gcctgtgacc gggctggtca
      181 aaccgctttt caggtagcga accgatgagc catccatcat ccctgtccgg ggcgtttcgc
      241 aagccctttc cgcagcagat cgcgttcttc cggaacaagc tgaagaacct ggtcccgacc
      301 cagcgctggg acgatctgac cggcgaggcg cacgataccg cgttcatggt ggcgggcgcc
      361 gccaaggccg acctgctgac cgatctggcg gcagcgacgg acaaggcgat cgccgagggg
      421 cgcgggatag aggagttccg gcgcgacttc cgcgatatcg tcaagcgcaa cgggtgggcc
      481 gggtggaccg gcgaaggttc gatcaagggc gaggcctggc gggtgaagac gatcctgcgg
      541 accaacgcct acaccagcta ttcggccggc cggcatgcgc agctgctgga cggcgaattt
      601 gcgttctggg tctatcgcca cggcgacgcg cgggaaccgc gcctggtcca cctgtcgttc
      661 aacggcctgg cgctgccgcc cggccatccg ttctggggca agtattaccc gccgagcgac
      721 tggggctgct cgtgctatgt cgtgggcgcg cggaccgctg ccggagtgcg gcgcctgggc
      781 ggcgatccgg gcaaggcgct gccggcgaac tgggcggcgc tcgatccgag gaccggcgag
      841 ccgaagggca tcggcaaggg ctggggctac gcgcccggag cgagcgtcgc gccggtcgtc
      901 caggcgctgg cgggcaagat cggaagctgg gattaccgca tcgccaaggc gttcatggaa
      961 ggcgtgccgg aagcgcagcg cgatgccctg gcacaggcgt accgatcgct gccaagcacg
     1021 gcggacgacg tgcgacgcta tgcccagcgc gtttatgaac cgaagcccga tctggccgag
     1081 ctgccgccgc agcggacgct cgggctgatc ccgggcgacc aggcggcgac gatcggcaag
     1141 ctgattgacc gggagattgc cggatatgat ttcagcatcg atcaatccgg cgtggggcat
     1201 gtgatcgggg agcacggccc cgggaatgaa acggacaaaa agcagcgggc ggtggactca
     1261 gcggacttcg cccggctgcc aatggttctg caatcgccgg gcagcgtcac cctgagcgac
     1321 aagctctccg acatgggcga gacagtcatc gattacacca aggcgatcga tggcgaagag
     1381 tggcacgtgc ggatggcagt gcgaggcaag aagcggcgga cgctcgcgct caagaccatg
     1441 ttcattgtga tcgggaaatg acgcgccccc cggcttacgt ccatcacgcc ttcccggtta
     1501 tcggccgggc gcccggatgc gcgtcgtgac ggagagatag cgatgttcaa cgtccagttc
     1561 aacgccggcg aaagccgcga ggcgatccgc cgggcgatcg cggcgctgga agacatgacc
     1621 ccgctgttcc aggacgtggc cgagtacatg accgaggcca cgcgccagcg gttcgtgacg
     1681 ggcaccgctc cggacgggtc gaagtggccg gccaagagcc aggtaacgct cgatcgctac
     1741 aagcggcttg gctacggcaa cctcagccgt gtgctgatcg gtcccggcaa gcggctgtcg
     1801 cgcgagatcg tgagcgaggc gaccaagggc ggcgcggtga tcggttcggc gctgatctat
     1861 tccagcgtga tgcaggatgg cgcgccgagg ggcgcgttcg ggaccaacag ccgcggccgg
     1921 ccgatcccgt ggggagcaat cccggcgcgg acctggctcg gcatctcgag cacggacgcg
     1981 gaccagatcg tcgccatcgc cgaggagcat atcgcggcga gcgtcgacgg ggcggaaaag
     2041 ggccgctgag cgcggtcggc tccggcgggg ccgaacccgc ttgacctgac gccgccgagc
     2101 ggcaattcag gaggcggagc cgggatcggc tttcacggcc gccggggcgg ccatattttc
     2161 cgggtgcacg agcgggcaaa ccgctttcga tgacgcggac agccagccag atcgcattgt
     2221 gttccgccgt cgcattcgcg gcggccgaag acgtgccgga gtgggtccac ctgctcccgg
     2281 ccggcgaaat ccgcacggtc gacgggcgcg gtccttatcg cgtcaagtcg ctccagaacg
     2341 tgatggcaag gtcgctccag ggcggcgcca agctgccgat cgacgaatgt cacgtgaccg
     2401 acaagggcgc tgcgctgggg cttgctgcac cggcccgggg ctggatcgtg gaactgcagg
     2461 cgcgcgagga tggcgtctgg gggcgggtcg agtggaacag ctcgggccgg cagctgatgg
     2521 ccgacaaggc ctacatcggg ctcagcccgg tcatcctcca ttcgcggggc ggcgacgtga
     2581 tgcagctcct gcgggcaagc ctgaccaaca caccaaacct gcagggcctg acaaccctgc
     2641 attcggagag caacaccatg gattggaaag cgaagctgat cgagctcctc gggctcgaca
     2701 gcaacgccga tgacgcggcg atcgaagccg cgctcacggc gaaaatgagc ggcggcgaca
     2761 ag
//
LOCUS       DATNBV010000258         2480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_794543,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000258 DATNBV010000000
VERSION     DATNBV010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794543"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="VN627_06195"
     CDS             <1..1511
                     /locus_tag="VN627_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HXD02882.1"
                     /translation="HGQANYPAMTVGPLNPTAVDHSTDAWDTIDWLVKNVPESNGRVG
                     TFGTSYNGFTALMSLVNPHPALKASVPINPMVDVWKGDDWFHNGAFRQEMIGYVYGQT
                     SGRTSVSEYFPSLRDDYTAALRLGSAGAYGRAFGMDRLPFWRRLTEHPAYDSYWQDQA
                     LDRILGSRPLTVPTMIVGSLWDQEDIYGATALFNSLKGSLNARFVLGPWYHGQANYPA
                     MTVGPLNWGIDTARWFRQRLLIPFLDEHLKGGPPAGIARVTAFEAGANRWDRLNDWPL
                     ACAHGCPANLTPLYLASGKGLSFTAAPGASLTYVSDPARPVPYRAPPITAPFAPNSTW
                     QTWLTDDQRFAERRPDVLTFATAALDKPLRLAGTPLVHLVASTSGSDSDWVVKLIDVH
                     PAASRLAGYELPIAMEIFRGRYRLDPARPAPLAPNQPLTYEFALPTVDYVVQPGHRIM
                     VQVQSSWFPLYDRNPQTFVPNIFFATRKDYRAATQRIFTGASGSWIGLPVVK"
     gene            1559..1631
                     /locus_tag="VN627_06200"
     tRNA            1559..1631
                     /locus_tag="VN627_06200"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1592..1594,aa:Trp,seq:cca)
     gene            1742..1939
                     /gene="secE"
                     /locus_tag="VN627_06205"
     CDS             1742..1939
                     /gene="secE"
                     /locus_tag="VN627_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HXD02883.1"
                     /translation="MARTSPGEFIRQVRIETSKVHWPTRKETIATGIMVMIMTLVLAF
                     FFFGVDAAFSTLVKKLLSFLA"
     gene            1962..>2480
                     /gene="nusG"
                     /locus_tag="VN627_06210"
     CDS             1962..>2480
                     /gene="nusG"
                     /locus_tag="VN627_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HXD02884.1"
                     /translation="MSRWYIIHAYSGFENKVRDAIMSEATRMGLTPFVEQIEVPTETI
                     TEIKRGKKVQAERKFMPGYVLAKLEMNDQVYHLVKNTPKVTGFLGPGGKPQPIPDAQA
                     ARMLDTKEEAAAHGAKQKINVDYEIGDAVKVLDGPFATFNGVVEELDFDRGRVKVSVS
                     IFGRATPVELEFE"
BASE COUNT          447 a          925 c          700 g          408 t
ORIGIN      
        1 atcacggcca ggcaaactac ccggcgatga ctgtcgggcc gctcaacccg actgcggtcg
       61 accattcgac cgacgcttgg gacacgatcg actggctggt gaagaacgtg cccgagtcca
      121 acggccgcgt cggcaccttc ggcaccagct acaacggctt caccgcgctg atgagcctgg
      181 tgaacccgca ccccgcgctg aaggcgagcg tgccgatcaa cccgatggtc gacgtgtgga
      241 aaggcgacga ctggttccac aacggcgcct tccgccagga gatgatcggc tacgtctacg
      301 gccagaccag cggccgcacc tccgtcagcg aatatttccc gagcctcagg gacgactaca
      361 ccgccgcgct gcgcctcgga tcggcgggcg cctatggccg cgcgttcggc atggatcgcc
      421 tgcccttctg gcgccgcctg accgagcatc ccgcctatga ctcctactgg caggaccagg
      481 cgctcgaccg catcctcggc agccgcccgc tcacagtccc gacgatgatc gtcggcagcc
      541 tgtgggacca ggaggacatc tacggcgcga ccgcattgtt caattcactg aagggcagcc
      601 tcaacgcgcg gttcgtcctc ggcccctggt atcacggcca ggcaaactac ccggcgatga
      661 ctgtcgggcc gctcaactgg ggcatcgaca ccgcgcgctg gttccgccag cgcctgctga
      721 tccccttcct cgacgagcat ctgaagggcg gcccgccggc cggcatcgcg cgcgtcaccg
      781 ccttcgaagc cggagccaat cgctgggacc ggctcaatga ctggcccctc gcctgcgcgc
      841 acggctgccc ggcgaatctc acccccctct acctcgcgtc cggcaagggc ctgagcttca
      901 ccgcagcccc cggcgcctca cttacctacg tctccgatcc cgcgcggcct gtcccctacc
      961 gtgcgccgcc gatcaccgcg cccttcgcgc ccaattcgac ctggcagacc tggctgaccg
     1021 acgaccagcg cttcgccgaa cgccgcccgg acgtcctcac cttcgccacc gcggcgctcg
     1081 acaagccact gcgcctcgcc ggcacgcccc tcgtccacct cgtcgcctcg accagcggca
     1141 gcgacagcga ctgggtcgtc aagctgatcg acgtccatcc ggccgcctcg cgccttgccg
     1201 gctatgaact gccgatcgcc atggagatct tccgcggtcg ctaccgcctc gaccccgcac
     1261 gccccgcgcc gctcgcgccc aaccagccgc tgacctacga gttcgcgctc ccgacggtcg
     1321 actatgtcgt acagcctggc caccgcatca tggtccaggt ccagtcgagc tggttcccgc
     1381 tctacgaccg caacccgcag accttcgtgc ccaatatctt cttcgcgacc cgcaaggact
     1441 atcgcgccgc cacacaacgc attttcaccg gcgccagcgg cagctggatc ggcctccccg
     1501 tagttaagtg accttgcgcc gcagaatgac ggtcgctacc tcgccgcggc gcgctcatag
     1561 gagtgtagct cagttggtag agcatcggtc tccaaaaccg agggccgggg gttcgagtcc
     1621 ctccactcct gcgcgcgaca accttgaaga ctcacccaac cgccgctaag tgagccgcag
     1681 ccccgccggc ccgaaaggtc cgcggggcgc ttttctatcc gcaactcgag aagacgaacc
     1741 agtggccaga acctcccccg gcgaattcat ccgtcaggtc cgcattgaga cctccaaggt
     1801 ccattggccg acccgcaagg aaaccatcgc caccggcatc atggtcatga tcatgaccct
     1861 ggtcctcgcc ttcttcttct tcggcgtcga cgccgccttt tcgaccttgg tcaagaagct
     1921 cctgagcttc ctggcctagc taaatccaag agaaacgatc catgtcccgc tggtacatca
     1981 tccacgctta ttccggcttc gagaacaagg tccgcgacgc gatcatgagc gaggcgacgc
     2041 gcatgggcct cacgcccttc gtcgagcaga tcgaggtccc gaccgagacc atcaccgaga
     2101 tcaagcgcgg caagaaggtt caggccgagc gcaagttcat gcccggctac gtcctcgcca
     2161 agctcgagat gaacgaccag gtctatcacc tcgtcaagaa cacgccgaag gtgaccggtt
     2221 tcctcggccc cggcggcaag ccgcagccga tcccggatgc tcaggccgcg cgcatgctcg
     2281 acaccaagga ggaggccgcg gcccacggcg ccaagcagaa gatcaatgtc gattatgaga
     2341 tcggcgatgc agtcaaggtg ctcgatggcc ccttcgcaac cttcaacggt gtggtcgagg
     2401 agctcgactt cgaccgtggc cgcgtgaagg tttcggtctc catcttcggg cgggctactc
     2461 cggtggaact cgaattcgaa
//
LOCUS       DATNBV010000259         6033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2075056,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000259 DATNBV010000000
VERSION     DATNBV010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075056"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VN627_06215"
     CDS             complement(<1..276)
                     /locus_tag="VN627_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXD02885.1"
                     /translation="MTKPKVLISDKMDPNAARIFTEMGCDVDVITGETPEQLIARIGE
                     YDGLAIRSSTKVTKEIIDAATKLKVIGRAGIGVDTIDVPAATQRGIVV"
     gene            complement(424..1545)
                     /locus_tag="VN627_06220"
     CDS             complement(424..1545)
                     /locus_tag="VN627_06220"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HXD02886.1"
                     /translation="MTVTKPALPERPFFSSGPCAKAPTFSPDKLATESLGRSHRAKIG
                     KQRLQYCIDLMREVLGLPDTHRIGIVPGSDTGAFEMAMWTMLGARPVTTLAWESFGEG
                     WVTDAAKQLKLDPTVLRADYGQLPDLTAIDWSSDVLFTWNGTTSGVRVPNADFIPANR
                     EGLSFADATSAVFAYDIDWSKIDVATFSWQKVLGGEGGHGVLILGPRAVERLESYTPA
                     WPLPKVFRLVSKGKLAEGVFKGETINTPSMLAVEDAIFALEWAKDIGGLEGLKARCTA
                     NAEALDKIVAERSWLSHLAADEATRSKTSVCLSVEGADADFIKQFAGLLEKEGAAFDI
                     AGYRDAPAGLRIWCGATVNVADIEALGPWLDYAWAQLKA"
     gene            1877..2215
                     /locus_tag="VN627_06225"
     CDS             1877..2215
                     /locus_tag="VN627_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02887.1"
                     /translation="MLNKVAGLARAVGLILAVVAAFVAIPAQVPLILVVLGIIAGLAY
                     SSEDIVRLAITAVALPLVGAALAVIPQIGTQLTAVAGNAALAVAGALATRVAVRLYEL
                     VVGDLKGLAS"
     gene            2335..3024
                     /locus_tag="VN627_06230"
     CDS             2335..3024
                     /locus_tag="VN627_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HXD02888.1"
                     /translation="MRRGPALPSALLALAALLPGLLSGCGGMVPQEAAREPLHRSTQL
                     YSPRPEAGACLASLGRSNASFMPLPDQYFGAHCSALNAVRLVSLRSDTASLGVSNLGP
                     VACPLAETFAAWARFGVDRAAGQILGSRLARIETMGSYNCRTVAGTDRMSAHATAQAI
                     DVAGFVLADGRRYTVLRDWNSPDPQVRAFFATIEQSACKRFGTVLGPAYNPAHRDHLH
                     VELGNGRNFCR"
     gene            complement(3064..3804)
                     /locus_tag="VN627_06235"
     CDS             complement(3064..3804)
                     /locus_tag="VN627_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HXD02889.1"
                     /translation="MTYCVGMMLDRGLVLMSDTRTNSGVDNISVFRKMFHWEVPGERI
                     IAIMTAGNLATTQQVISQLEERTKAPGDRHNCLLEAPTMFQVASLVGKLLGETIQQRE
                     DFNGEEGQGRFTASIIVAGQIKGMAPRLFMIYPEGNFIEASYDTPFFQIGETKYGRPI
                     ILRGYDRAMTFEDAVKLLMVSFDSTLKANLSVGLPLDLLVIEKDRFEPLHSRRITAAD
                     PYFQQISSGWGEALKRAFYSLPDYSFGE"
     gene            complement(3875..4690)
                     /locus_tag="VN627_06240"
     CDS             complement(3875..4690)
                     /locus_tag="VN627_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HXD02890.1"
                     /translation="MRLAVCHVTRYRFDPPLAHGLQRLRLSPKETHGQQVREWRMQLA
                     GGRVEASYEDHNANHVTLVSLEPGTAELAITCTGLVDTADRAGVLGPHTGRLPLWAFL
                     AQTPLTRPGARMRALATGLGGDRADVLATLHALSDAVLGAAAYEVGHTDSATTAEEAL
                     AAGKGVCQDHAHIFIGCARQLGIPARYVSGYLMMDDRIEQEAGHAWAEAHVAGLGWVG
                     FDVSNGISPDARYVRVASGRDYAEAAPVTGITFGAGVANLSVSLAVEQQAVQQ"
     gene            complement(4699..5643)
                     /locus_tag="VN627_06245"
     CDS             complement(4699..5643)
                     /locus_tag="VN627_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HXD02891.1"
                     /translation="MLARTANGVFWMARYLERAENSARLLDAGFRIALTRGDHASADE
                     WRSVLVTIGLEHAFAARGLDYTGPAVCNFILRDKDNPASVLAMIEQARTNARMVRTAI
                     TREVWEATNEAWMTLCEVLARPVREANLGDTLALIRRQTTQVRGAMDGTMLRNEIFNF
                     ARIGTFIERADNTARILDVKYHVLLPSVAWVGSSLDHAQWETVLRSVAGDRAYRWLNA
                     GRLDARGIAQFLILDGRFPRSLAFCYDKLRSNMAGLAREYGGETRAHVLLQTARDRLH
                     QAPIEKIIEAGLHEFIVGFIAQTLEIAQAIAIDYRFIE"
     gene            complement(5759..>6033)
                     /locus_tag="VN627_06250"
     CDS             complement(5759..>6033)
                     /locus_tag="VN627_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HXD02892.1"
                     /translation="RKEIAAFAAKLRARPENYIAQPTLALSTCPILTRAGLAPRHVDL
                     RPFVLMSPGGIEITPGGLTRVALKKGSLVVNSSQGGGTKDTWVLED"
BASE COUNT          979 a         1903 c         2133 g         1018 t
ORIGIN      
        1 caccacgatg ccgcgctgtg tcgcggccgg cacgtcgatc gtgtcgacgc cgatgccggc
       61 gcggccgatc accttgagct tggtggcggc gtcgatgatt tccttggtga ccttggtcga
      121 cgagcggatg gcgaggccgt cgtattcgcc gatacgggcg atcagttgct ccggcgtttc
      181 gccggtgatg acatcgacgt cgcagcccat ctcggtgaag atgcgggcgg cgttgggatc
      241 catcttgtcg gaaatgagaa ccttgggctt ggtcatggaa tgcactccat tgctccattc
      301 ccgttcgccc tgaggagcga ggacacagtc cgagcgtctc gaagggtcct tcgagacggc
      361 gcttcagcca ttcggcttca gcgcctcctc aggacgaacg ggggagatta aaacagcggg
      421 gattcaggcc ttcagctggg cccaggcata gtccagccag ggtccaagcg cttcgatgtc
      481 ggcgacattc acggtcgcgc cacaccagat cctgaggccg gccggggcat cgcgataacc
      541 cgcgatgtca aaggcggcgc cttccttctc gaggaggccg gcgaactgct tgatgaagtc
      601 cgcatcggcg ccttcgaccg agaggcagac cgaggtcttc gaccgggtcg cctcgtcggc
      661 agcgaggtgc gacagccagg agcgttccgc gacgatcttg tcgagcgcct cggcgttggc
      721 ggtgcagcgg gccttcaggc cctcaagccc gccaatgtcc ttggcccatt cgagcgcgaa
      781 gatcgcatcc tcgacggcga gcatcgaggg ggtgttgatc gtctcgccct tgaacacgcc
      841 ctcggcgagc ttgcccttgg agacgaggcg gaacaccttg gggagcggcc aggccggggt
      901 atagctctcg agccgctcca cggcgcgggg accgaggatg aggacgccgt ggccgccctc
      961 gccgccaagg accttctgcc aggagaacgt ggcgacgtcg atcttcgacc agtcgatatc
     1021 ataggcgaag acggcgctgg tggcatcggc gaaggacagg ccctcgcggt tggcggggat
     1081 gaaatcggcg ttggggacgc ggacccccga ggtcgtgccg ttccaggtga acaggacgtc
     1141 gctggaccag tcgatcgcgg tcaggtccgg gagctggcca tagtcggcgc gcaggaccgt
     1201 cggatcgagc ttgagctgct tggcggcatc ggtgacccaa ccttccccga acgattccca
     1261 ggcgagcgtg gtgaccggac gcgcgccgag catggtccac atcgccatct cgaaggcgcc
     1321 ggtgtccgaa cccggaacga tgccgatgcg gtgcgtatcg ggcagcccca gcacttcgcg
     1381 catcaggtcg atgcagtact ggaggcgctg cttgccgatc ttggcgcggt gcgagcggcc
     1441 gagcgattcg gtggcgagct tgtcggggct gaaggtgggc gccttggcgc agggtccgga
     1501 cgagaagaaa ggacgctccg gaagcgccgg cttggtaaca gtcatgtagt ctctccttgc
     1561 agagagcacg cgcggcgttg ggaccgcgtg gcccggcggc ggcaatagtg atgcggtgca
     1621 gcatgtcaag cgcgctattt cggcggcagg ggtccggcat ggtcccggac cgcccctcgt
     1681 gccgggcgat ccgccgccaa tgctgcgcac aatctcaggc gtggataacc ttagccatga
     1741 tctcaaaact ccacgttttc cgggctttgc tgcgcaacat aaagaggttg ccaatgaaac
     1801 ctgttcagct aggctcccgc acgtcccgaa cggggcaacg aacataatgt cgcaagcaaa
     1861 aatggagggg aacatcatgc tgaacaaggt cgccggcctc gcccgggcgg tggggctgat
     1921 actcgcagtc gtcgccgcat tcgttgccat cccggcccag gtgccgctga tcctggtcgt
     1981 gctgggcatc atcgccgggc tcgcctacag ctccgaggac atcgtgcggc tggcgatcac
     2041 cgccgtggcc ctgccgctcg tcggcgccgc gctggcggtc atcccgcaga tcggcaccca
     2101 gctgaccgcc gttgcgggca atgccgccct cgccgttgcc ggcgccctcg ccacgcgggt
     2161 cgcggtgcgg ctctatgaac tcgtcgtcgg cgatctcaag gggcttgcca gctgatcgcc
     2221 tgaaccagcg aagggccggg cccggccggg acagcagtgc ccggggcccg gccctttctg
     2281 attcgagctt ccgatttacc atttgtttac catggccgtg ggatcatcgc ggccatgcga
     2341 cgcggtcctg ccctgccatc ggcgctgctg gcgctggctg ccctgcttcc gggcctgctc
     2401 tccggctgcg gcggcatggt tccgcaggag gccgcgcgcg aaccgctgca ccgctcgacc
     2461 cagctctatt ccccccggcc cgaagccggc gcctgccttg cctcgctggg gcggagcaat
     2521 gcgagtttca tgccgctgcc cgaccagtat ttcggcgcgc actgttcggc gctcaatgcg
     2581 gtcaggctgg ttagcctgcg cagcgacacc gccagccttg gcgtgtcgaa cctcggcccg
     2641 gtggcctgcc cgctggccga gaccttcgcc gcctgggccc gcttcggggt ggaccgcgcc
     2701 gccgggcaga tcctcggcag ccgccttgcc cggatcgaga cgatggggtc ctacaactgc
     2761 cggaccgtgg ccggaaccga ccggatgagc gcccatgcca cggcccaggc gatcgacgtg
     2821 gccgggttcg tcctggccga tggacggcgc tataccgtgc tcagggactg gaactcgccc
     2881 gacccccagg tgcgcgcgtt cttcgccacg atcgagcaaa gcgcctgcaa gcggttcggc
     2941 acggtgctgg gaccggccta caacccggcg caccgcgatc acctgcatgt ggagctgggg
     3001 aacgggcgga acttctgccg gtaaggcgac gtcgtcccgg atcgagcccg ggacgatgcc
     3061 ggctcactcc ccgaaactgt aatccggcag cgaatagaag gcgcgcttga gggcttcgcc
     3121 ccagccggag ctgatctgct ggaaataggg atcggcggcg gtgatgcggc gggagtggag
     3181 cggctcgaaa cggtccttct cgatcaccag cagatcgagc gggaggccta cggagaggtt
     3241 ggccttgagg gtggaatcga agctgaccat gagcagcttg accgcgtcct cgaaagtcat
     3301 cgcccggtca tagccacgca ggatgattgg gcggccatac ttggtctcgc cgatctggaa
     3361 gaagggcgtg tcgtagctcg cctcgatgaa gttgccttcg gggtagatca tgaacaggcg
     3421 cggggccatg cccttgatct ggccggcgac aatgatcgag gcggtgaagc ggccctgccc
     3481 ttcctcgccg ttgaaatcct cgcgctgctg gatcgtctcg ccgagcagtt tgccgacgag
     3541 gctggcgacc tggaacatgg tcggcgcctc gagcaggcag ttgtgacgat cacccggcgc
     3601 cttggtgcgt tcctcgagct ggctgatcac ctgctgggtg gtggcgaggt tgcccgcggt
     3661 catgatcgca atgatccgtt cgccgggcac ttcccagtgg aacatcttgc ggaacaccga
     3721 gatgttgtcg acccccgaat tggtgcgggt gtcgctcatc agcacgagcc cgcgatcgag
     3781 catcatgcca acgcaatagg tcattcttcc aaccccaatc cttggctgca acaggcgaac
     3841 gcgccgaccg gcgattcgcc tcccctgctg gccgttactg ttgaacggcc tgctgctcca
     3901 ccgcaaggct gaccgacaga ttggccacac ccgcgccgaa ggtgatcccg gtgaccggcg
     3961 ccgcttcggc atagtcgcgc cctgaggcga cgcggacgta gcgggcatcg gggctgatcc
     4021 cgttggagac gtcaaacccg acccagccga gaccggcgac gtgggcttcg gcccaggcgt
     4081 ggccggcttc ctgctcgatc cggtcgtcca tcatcaggta gcccgagaca tagcgcgcgg
     4141 gaatgccaag ctggcgggcg cagccgatga agatgtgagc atgatcctgg cagacgccct
     4201 tgccggcggc gagcgcttcc tcggccgtgg tggcgctgtc ggtatgaccc acttcgtagg
     4261 cggcggcgcc gagcaccgcg tcggagaggg catggagcgt ggcgagcaca tcggcgcgat
     4321 cgccgcccag cccggtggcg agggcgcgca ttcttgcccc cggccgggtc agcggggtct
     4381 gcgcgaggaa agcccacagc ggcaagcggc cggtgtgggg gccgagaacg ccggcacggt
     4441 cggcggtatc gaccaggccg gtgcaggtga tggcgagttc ggccgtgccg ggttcgaggc
     4501 tgacgagggt cacgtggtta gcgttgtgat cttcgtagct ggcctccacc cggcctccgg
     4561 caagctgcat gcgccattcg cggacctgct ggccgtgggt ttccttcggc gacagacgca
     4621 ggcgctggag gccgtgggcg agcgggggat cgaaccggta gcgggtgaca tggcagacgg
     4681 cgaggcgcat gggcgggcct actcgatgaa ccggtagtcg atggcgatcg cctgggcgat
     4741 ctccagcgtc tgggcgatga aaccgacgat gaattcgtgc aggccggcct cgatgatctt
     4801 ttcgatggga gcctggtgaa gccggtcgcg cgcggtctgg agcaggacat gggcgcgggt
     4861 ttcaccgcca tattcgcggg cgaggccggc catgttgctg cgcagcttgt cgtagcagaa
     4921 ggcgagggag cgcgggaagc ggccatcgag gatcaggaac tgggcgatgc cgcgggcatc
     4981 gaggcgcccc gcattgagcc agcggtaggc gcggtctccc gcgaccgacc gcaggacggt
     5041 ttcccactgg gcatggtcga ggctggagcc gacccaggcg accgagggca gcaggacgtg
     5101 gtacttcacg tcgaggatgc gggcggtgtt gtcggcccgt tcgatgaagg tgccgatccg
     5161 cgcgaaattg aagatctcgt tgcgcagcat ggtcccgtcc atcgccccgc gtacctgggt
     5221 ggtctggcgg cggatcaagg cgagggtatc gccgaggttg gcctcgcgca cggggcgggc
     5281 gagcacttcg cacaaggtca tccaggcttc gttggtggcc tcccagacct cgcgggtgat
     5341 cgcggtgcgg accatgcggg cattggtgcg ggcctgctcg atcatggcga ggacggacgc
     5401 cgggttgtcc ttgtcgcgca ggatgaagtt gcacacggcc gggccggtat agtccagccc
     5461 gcgcgcggcg aaggcatgct caagcccgat ggtgacgagg accgagcgcc attcatcggc
     5521 cgaggcatga tcgccgcggg tgagcgcgat gcggaaaccg gcatcgagca ggcgggcgct
     5581 gttttccgcg cgctcgaggt agcgggccat ccagaacacg ccgttggcgg tgcgagctag
     5641 catgggcggc cgctccgggc caggaggacg tggcgcgagg ctaccaccat tccatccggc
     5701 tgaaacacgt tcagcagggc gggggctatc gacgcaccgg agggagcaga aaccaccatc
     5761 agtcctccag cacccaggtg tccttggtgc cgccgccctg cgacgaattc accacgaggc
     5821 tccccttctt gagcgccacg cgggtcagcc cgcccggcgt gatctcgatt ccgccgggcg
     5881 acatcagtac gaaaggcctg agatcgacgt gacgcggagc aagaccggcg cgggtgagga
     5941 tcgggcaggt ggaaagcgcg agggtgggct gggcgatgta gttttccggc cgggcgcgca
     6001 gcttggcggc gaaggctgcg atctccttgc ggg
//
LOCUS       DATNBV010000260         3099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_463438,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000260 DATNBV010000000
VERSION     DATNBV010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463438"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VN627_06255"
     CDS             complement(<1..240)
                     /locus_tag="VN627_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02893.1"
                     /translation="MKPITRLACSVAAIALGGAELAAQPPQPSDAAQVRAERTAAQMT
                     QDEKIQLIHSTFGVALPQAKRPADMPIGAGAIPGIP"
     gene            412..1098
                     /locus_tag="VN627_06260"
     CDS             412..1098
                     /locus_tag="VN627_06260"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXD02894.1"
                     /translation="MEAAPTNSGNTVALRDEAATKPHRPLRGRPTAQQVASIDRAIVQ
                     AARDMFLADGFDAVGMKQVAERARVSKGTLYSRYPSKEVLFTAVIEQTVEDWSAEAAQ
                     GDALLTDDIAQRLRHHARTIASWLGRPDVLALQRLLVSMRERFPAPAETMRRRGYDYI
                     VGLIARDIRQAAARDGTTLRDPLAVARMLVAAITGAHMQEDADGTGEEALQAFAQRVV
                     DVIIGGQGNW"
     gene            1175..1495
                     /locus_tag="VN627_06265"
     CDS             1175..1495
                     /locus_tag="VN627_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02895.1"
                     /translation="MIKFAIAAALAAITLPAAAGATEPAKTAAQAPAAKQAYTTADTD
                     IGTLLDDPAAKAIVEKYIPGLTTNPQVDMARAMTLKAVQAYAPDEVTDERLAKIDADL
                     AKLK"
     gene            complement(1581..2663)
                     /locus_tag="VN627_06270"
     CDS             complement(1581..2663)
                     /locus_tag="VN627_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02896.1"
                     /translation="MLDDARRQYLSDLLKPASPPREIASPFTEEQKRVMLDVVHDNGP
                     WKLILAQHFPTAESLLATFAGGFPEGFTPTLDMFLTPTFRGFLANYSAVLYPELHDIF
                     YNAAFLEHAKSYWGAQYAKPQMMLFNINGPCGNTDPGHLDSPSFRGVRYENSPTWLCS
                     IMGRSGLFQDYLIKMAQVITWFSHDPHSGFTYWPQGPLRKPARVQSPIYNRAVVVQNE
                     MLVHRGEGNGPVERRRPDGLAFESVFTGDPADRDGWLVKTGDRVIERYHTDELRFLVH
                     WSAEVFSDFDELKKNMEGSDDLTHEQAIDMLIKDVRSRGIQIETPTDPLNDPVFIRAL
                     NAAYDFGGPAEYPAEAPLDRPYEAAA"
     gene            2812..>3099
                     /locus_tag="VN627_06275"
     CDS             2812..>3099
                     /locus_tag="VN627_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXD02897.1"
                     /translation="MSVQEMTKGQRTAGRILDVAEELFAQHGYSATSLRDIAEQAGLQ
                     QPGLYKHFAGKEDLYRRVYDRALQPMTDLMDGILAGSDVGFGELAERMTDLL"
BASE COUNT          550 a          991 c          989 g          569 t
ORIGIN      
        1 ggggatcccg ggaatggcgc cggcacctat gggcatgtcg gcggggcgtt tcgcctgcgg
       61 cagcgccacg ccgaaggtgc tgtggatcag ctggatcttt tcatcctgcg tcatctgcgc
      121 ggccgtgcgt tcggcccgca cctgggccgc atcggacggc tgcggcggct gcgcggcaag
      181 ttccgcgccg cccagcgcga tcgccgcgac agagcatgcc aggcgcgtga ttggcttcat
      241 cgtgcttatc cgatcctgtc ccaacgacct gctgcgaatg gtctggtttc gccggccaga
      301 ttgtcctcgt ggccccctct tgtcaataaa tagatcacac gggttcgatt attcactttg
      361 gccggaccgg ttcgcttgcg cggcggcaag ggctgtgaaa gaagccgctc catggaagcc
      421 gcaccaacaa actccgggaa cacggttgcc ctccgtgacg aggccgcgac aaagccgcat
      481 cggccactcc gcgggcggcc gactgcgcag caggttgcat cgatcgaccg ggcgatcgtc
      541 caagctgccc gcgacatgtt cctggcagac gggttcgatg cggtagggat gaagcaggtg
      601 gccgaacggg cccgcgtgtc caagggcacg ctctattcgc gctacccttc gaaggaggtc
      661 ctgtttaccg cggtaatcga acaaactgtc gaagactggt cggccgaagc ggctcagggc
      721 gacgcgctcc tgaccgacga catcgcccag cggctccgcc accacgcgcg gactatcgcc
      781 tcctggctcg gccgccccga cgtgctcgcc cttcagcgcc tgctggtgtc catgcgcgaa
      841 cggtttcccg cgccggctga gaccatgcgc cgacgcggtt atgactatat cgtcggactg
      901 atcgcccgcg acatccggca ggctgcagcc cgcgatggca ccaccttgcg cgatccgctc
      961 gccgttgcgc ggatgctggt agcggccatt accggagcgc atatgcagga agatgccgac
     1021 gggacgggcg aggaagctct ccaggctttt gcccagcggg tcgtcgacgt gatcattggc
     1081 gggcagggca actggtagtc ttgatattcg aacacaagtg tgcatttaat atcgggcgag
     1141 cgactcacgc cgttccaatc ccgagaggat gtacatgatc aagtttgcca tcgccgcggc
     1201 ccttgccgcc atcacgcttc ccgccgccgc cggtgccacc gagccggcca agaccgctgc
     1261 ccaggctccg gccgccaagc aggcctacac cactgccgat accgacatcg gcaccctgct
     1321 tgacgatccg gccgccaagg cgatcgtcga gaagtacatt cctggtctga cgaccaaccc
     1381 gcaggtggac atggcccggg cgatgaccct taaggcagtc caggcctatg cgccggatga
     1441 agtgaccgac gaacgcctgg ccaagatcga cgccgatctc gccaagctca agtaagcttt
     1501 ccgttataaa aaagggtccg caaccttctg gctgcggacc cttttttgtg cctgggagat
     1561 ggcgttgaag cgggcccgcc tcaggcagcg gcttcgtatg gccggtccag cggggcttcg
     1621 gcggggtatt cggccggtcc gccgaagtcg tacgccgcat tcagcgcccg gatgaacacc
     1681 ggatcgttca gcgggtcggt tggcgtctcg atctggatcc cgcgcgaccg cacgtccttg
     1741 atcagcatgt cgatcgcctg ctcatgggtc aggtcatccg aaccttccat gttcttcttc
     1801 aactcgtcga aatcgctgaa cacctcggcc gaccagtgga cgaggaagcg cagttcgtcg
     1861 gtgtgatagc gctcgatcac cctgtcgcca gtcttcacca gccagccatc gcgatcggcc
     1921 ggatcgccgg tgaacacgct ctcgaaggcc aggccatccg gacggcggcg ctccaccggg
     1981 ccattgcctt cgccgcgatg gaccagcatc tcgttctgga cgaccacggc gcggttgtag
     2041 atcggcgact ggacgcgcgc cggcttcctc agcggccctt gcggccagta ggtgaacccg
     2101 ctgtgcggat cgtggctgaa ccaggtgatt acctgggcca tcttgatcag gtaatcctgg
     2161 aacaggccgg agcggcccat gatcgagcaa agccacgtcg gcgaattctc gtagcgcaca
     2221 ccgcggaagc tgggcgaatc gaggtggccc gggtcggtgt tgccgcaggg gccattgatg
     2281 ttgaacagca tcatctgcgg cttggcatac tgcgcacccc agtacgactt cgcatgctcc
     2341 aggaaggccg cgttgtagaa tatgtcgtgc aactcggggt agagcacggc cgaatagttg
     2401 gcgaggaatc cgcggaacgt gggggtgagg aacatgtcca gcgtgggcgt gaacccttcc
     2461 gggaagccgc ccgcgaaagt cgccagcagc gattccgcgg tggggaaatg ctgcgccagg
     2521 atcagcttcc acgggccgtt gtcgtggacc acgtccagca tcacccgctt ctgttcctcg
     2581 gtgaaggggc tggcgatctc ccgcggcggg gaggccggct tcaggaggtc ggaaaggtat
     2641 tgtctgcgcg cgtcgtcaag catcgccatc tcctcgggca atcaatcgcc ggcgcaattc
     2701 acgcctcggt gattcttcag aaaagttatg ggtcgataac ttttttcgca agaggttttc
     2761 gtcagactgc gctattgtca ccctggtccg agcgttggag ggcaggcagg cgtgtcagtg
     2821 caggagatga ccaaggggca gcgcaccgcg ggccgcattc tcgacgtggc ggaggagctg
     2881 ttcgcgcagc atggctattc ggccacttcc ctgcgcgaca tcgccgagca ggcggggctg
     2941 cagcagcccg gactctacaa gcacttcgcc ggcaaggaag atctctaccg ccgcgtttat
     3001 gaccgcgccc tgcagccgat gaccgacctg atggacggca tcctcgccgg aagtgacgtt
     3061 ggcttcggcg aactggccga gcgaatgacc gacctcctc
//
LOCUS       DATNBV010000261         4922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_170999,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000261 DATNBV010000000
VERSION     DATNBV010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170999"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VN627_06280"
     CDS             <1..917
                     /locus_tag="VN627_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative urea ABC transporter substrate-binding
                     protein"
                     /protein_id="HXD02898.1"
                     /translation="SGILKKWADKYGIKINLIQVNDYVESVNQYTAGKLDGVGVTNMD
                     ALTIPAAGGKDTTALIMGDYSNGNDGIVMKQGKSVADLKGQTINLVELSVSHYLLARA
                     LEMSGMKLADVKTVNTSDADIVSAFATPQVGTVVTWNPQLSEVKKSGTEVFDSSKIPN
                     EIQDLMVVSTPTLAANPALGKALVGAWFEVLALMQKGGPESEAAIAEMAKMSGTDVAN
                     YKGQLATTYLYYKPADAVAYVTGAEIMKHEDLVRQFSFSKGLFGPNATSVDAIGIELP
                     GGRVLGDKANVKLRYNADFMKQAADGKL"
     gene            1105..2778
                     /locus_tag="VN627_06285"
     CDS             1105..2778
                     /locus_tag="VN627_06285"
                     /inference="COORDINATES: protein motif:HMM:NF005511.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXD02899.1"
                     /translation="MTQVPITDSVPTGTPVGEPLRVASADAIQWDATCDLLVVGIGLA
                     GASATLRGSELGLDTIAVDRFQGGGASELSGGVVYAGGTHVQAELGIEDSVENLYNYI
                     GHETGDLISEPTLRKFCQDSPGQITWLEKYGVPFGGPVAMRKASYPPRGHYLYYSGNE
                     TSPEGKARATPAPRGHRAQPTFKTKERFSGLFIMSQLKKAIAEAGNVRRMYQAAARRL
                     VVDAQGRVIGAEIWQIPPGSVAALLHAKAYLAGRPLALEALKLNGPLWRTVGWLERKF
                     AKPKLVRARKGVVLSAGGFIHNKAMTDRHTPLYQNIFRQGTQGDDGSGIRIGVAAGAA
                     TSQMSTISAWRFVSPPYDWPKGIFVGPDGKRFTNEELYGARTSRAMFEKAGGRGWLIA
                     DQSLIDSALADIATGELQEFQVMVAKTWIRNAKKADTVEGLEQAMGMPAGSVSGEVEA
                     YNAAIAAGTTDPMGKSDYMRQALVKAPFYALDISNQTTAPLSALTMGGLSLDEATGSA
                     LREDGSLIPGLYAAGRNATGMCSNEYISGLALADCIWSGHRAAQSAAAS"
     gene            complement(2804..3553)
                     /locus_tag="VN627_06290"
     CDS             complement(2804..3553)
                     /locus_tag="VN627_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02900.1"
                     /translation="MDYISVSQARGLPGLRVAFTRGVPGPWGVSAMGILDLKGITYAA
                     VAQEAGRANAELQEWTGQSSAPVAMYEDERPRSGWAEILMLAERLAPEPRLVPADEDL
                     RAEMFGLCHEICAEDGLGWNLRLLMLAGKDSVKALPRLGHKYGSAAPLEHAARRVNAV
                     VALLARRLAAQRALRRPGFVGETLTAADIYWTAFSNLLDAMAPELCPMPDFYREMGAI
                     TSAVLEEPLPQLLLDHRDAVARSCFRLPIVQ"
     gene            complement(3626..4444)
                     /locus_tag="VN627_06295"
     CDS             complement(3626..4444)
                     /locus_tag="VN627_06295"
                     /EC_number="1.1.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl-alcohol dehydrogenase"
                     /protein_id="HXD02901.1"
                     /translation="MGVWSFEGKRVVIAGCFSGMGEAAARELVNLGAEVHGVDIKPSP
                     VNLASFTQVDLRDPASIDAAVAAIGGEIDCLFNCSGLPQTFPPMDVIKVSYIGMRYWT
                     EQWAPKIRKGGAIATITSTAGMGHMARAALHEELISQPSFDAAVAWVEQRLDTLGDCY
                     GFAKEAMQYWTKVASQEFIKQGVRINAIAPGPTQTPMMNDFEQVVGGAAVMDIYTQPI
                     NRRSTPAEQAYPLIFLNSDAATYVNGHILDADGGFTAGVMTGRIKMQMGDRETG"
     gene            4588..>4922
                     /locus_tag="VN627_06300"
     CDS             4588..>4922
                     /locus_tag="VN627_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXD02902.1"
                     /translation="MATAATTVDVPPHVTPDRVVDFDFYNDPRFAADDIHTAFAKMLK
                     QVPPVFWTPHNGGHWVIAGHEELFEASRNTELFSSAKGGLPPSPYEPSQLPISIDPPR
                     HAIYREPLAR"
BASE COUNT          871 a         1704 c         1575 g          772 t
ORIGIN      
        1 agagcggcat cctcaagaag tgggccgaca agtacggcat caagatcaac ctgatccagg
       61 tgaacgacta cgtcgagagc gtgaaccagt acaccgccgg caagcttgac ggcgtgggcg
      121 tcaccaacat ggacgcgctg accatccccg ccgccggcgg caaggacacc accgcgctga
      181 tcatgggcga ctattccaac ggcaatgacg gcatcgtcat gaagcagggc aagtcggttg
      241 ccgatctcaa ggggcagacg atcaatctcg tcgagctgtc ggtctcgcac tatctcctcg
      301 cccgcgcgct cgagatgagc gggatgaagc tggccgacgt gaagacggtc aacacctccg
      361 acgccgacat cgtctccgcc ttcgccaccc cgcaggtcgg caccgtggtg acctggaacc
      421 cgcagctttc cgaagtgaag aagagcggga ccgaggtgtt cgacagctcg aagatcccca
      481 acgagatcca ggacctgatg gtcgtctcca cgcccaccct cgccgccaac ccggccctgg
      541 gcaaggcgct ggtcggcgcc tggttcgagg tgctcgcgct gatgcagaag ggcgggccgg
      601 aaagcgaggc ggcgatcgcc gagatggcca agatgagcgg caccgacgtc gccaactaca
      661 agggccagct tgccaccacc tacctctact acaagcccgc cgacgccgtc gcctatgtga
      721 ccggcgccga gatcatgaag cacgaggacc tggtgcggca gttctccttc tcgaagggcc
      781 tgttcggacc caacgccacc tcggtcgacg ccatcggcat cgagctgccg ggcggtcgcg
      841 tccttggcga caaggccaac gtcaagcttc gctacaatgc cgacttcatg aagcaggccg
      901 ctgacgggaa actctgagcc gccgcttcgc cgcgcctttg cctccaccct ccggtgaggc
      961 agcaaactgg gccccggacc atcggtccgg ggccctttct tttgcgccac gcggaggcag
     1021 gggattgcgt ctcgccgacc tggcggtaaa tacacagccc tgcatacata aatccggcgc
     1081 tcgatggcgc agcgggagag tagaatgacc caggttccga tcaccgattc cgtcccaacc
     1141 ggaacgcctg tgggcgaacc actgcgcgtg gccagtgccg atgcgatcca gtgggatgcc
     1201 acgtgtgacc tgctggtggt cggcatcggc cttgccggcg ccagcgccac cctgcgcggc
     1261 agcgagctcg ggctcgacac catcgcggtc gatcgcttcc agggcggcgg cgccagcgag
     1321 ctcagcggcg gcgtggtcta tgccggcggc acccatgtgc aggcggagct ggggatcgag
     1381 gattcggtcg agaacctcta caactacatc ggccatgaaa ccggcgacct gatctccgag
     1441 ccgaccttgc gcaagttctg ccaggacagc cccggccaga tcacctggct ggagaagtac
     1501 ggcgtgccgt tcggcggccc ggtcgcgatg cgcaaggcgt cctatccgcc tcgcggccat
     1561 tacctttatt attcgggcaa cgagaccagc ccggaaggca aggcccgcgc cacgccggcc
     1621 ccccgcggcc accgcgccca gccgaccttc aagaccaagg agcggttctc gggcctgttc
     1681 atcatgtccc agctgaaaaa ggcgatcgcg gaggccggca atgtccgccg gatgtaccag
     1741 gcggcggcaa ggcggctggt ggtagatgcg cagggacggg tgatcggcgc cgagatctgg
     1801 cagattccgc ccgggtccgt cgccgcattg ctgcacgcca aggcctacct cgccgggcgg
     1861 ccactggcat tggaagccct gaagctgaac ggaccgctgt ggcggacggt gggctggctg
     1921 gaacgcaagt ttgccaaacc gaagctggtc cgcgcccgca agggcgtggt cctcagcgcc
     1981 ggcggcttca ttcacaacaa ggcgatgacc gaccggcaca cgccgcttta ccagaacatc
     2041 ttccgccagg gcacccaggg cgacgatggc tcaggcatcc ggatcggcgt tgccgcaggg
     2101 gcggccacct cgcagatgag caccatctcc gcctggcgct tcgtcagccc gccctacgat
     2161 tggccgaagg ggattttcgt cgggccggac gggaagcggt tcaccaacga ggaactctac
     2221 ggcgcacgta ccagccgtgc gatgttcgag aaggccggag gacggggctg gctgattgcc
     2281 gaccagtccc tgatcgacag cgcccttgcc gacatcgcga ccggggagtt gcaggaattc
     2341 caggtcatgg tcgccaagac ctggatccgc aacgccaaga aggctgacac ggtcgaaggg
     2401 ctggaacagg cgatgggcat gcccgccggt tccgtttccg gtgaagtcga agcctacaat
     2461 gcggcgattg ccgccggaac gacagacccc atgggcaagt cggactacat gcgccaggcg
     2521 ctggtcaaag cgccgttcta cgccctcgac atctccaacc agaccaccgc ccccctgtcg
     2581 gcgctgacca tgggcgggct cagcctcgac gaggctaccg ggtcggccct ccgggaggat
     2641 ggttcgctca ttcccggcct ctatgccgcc gggcgtaacg ccacgggcat gtgctcgaac
     2701 gagtatatca gcgggctcgc cctggctgac tgcatctggt cgggccaccg cgcggctcag
     2761 tcggccgcgg ccagctgagg cgccgggacg ggcggctgcc ggcctactgg acgatcggca
     2821 gccggaagca gctgcgggcc accgcatcgc ggtggtccaa cagcagctgc ggcagcggct
     2881 cctcgagcac ggccgaggtg atcgcgccca tctcgcggta gaagtcgggc atcgggcaca
     2941 gctcgggcgc catggcgtcg agcaggttgg aaaatgccgt ccagtagata tccgccgctg
     3001 tcagcgtctc gccgacaaag cccggccgcc gcagcgcccg ctgcgccgcc agccgccgtg
     3061 ccagcagggc cacgaccgca ttgacccgcc ttgcggcatg ttcgagcggc gcggcggagc
     3121 cgtatttatg ccccagccgc ggcagggcct tcaccgaatc cttgccggcc agcatcagca
     3181 ggcgcaggtt ccaccccagg ccgtcttccg cacagatctc gtgacacagg ccgaacatct
     3241 cggcccgcag gtcctcgtcg gccgggacca gccgcggctc gggcgcaagg cgttcggcca
     3301 gcatgaggat ttcggcccag cctgagcggg gccgctcgtc ctcgtacatg gcgaccggag
     3361 cagagctctg gccggtccac tcctgcagct cggcattggc ccggccggct tcctgggcga
     3421 cggccgcata ggtgatgccc ttgagatcga ggatgcccat cgcggacacc ccccaggggc
     3481 ccggcacgcc gcgggtaaag gcaaccctca gcccgggcag tccgcgcgcc tgcgacaccg
     3541 agatgtagtc catgcccgcg ctctccctcg cgcgccccaa acgggaaggg ggcggtcttc
     3601 cggccgcccc cttcccgctg gccggtcaac cggtctcgcg gtcgcccatc tgcatcttga
     3661 tgcggccggt catcacgccg gcggtgaagc cgccatcggc atcgaggatg tggccattca
     3721 cgtaagtggc cgcgtcggaa ttgaggaaga tcagcggata ggcctgctcc gccggggtcg
     3781 agcggcggtt gatcggctgg gtgtagatgt ccatcacagc ggcgccgccg accacttgct
     3841 cgaagtcgtt catcatcggg gtctgcgtcg gccccggggc gatcgcattg atccgcaccc
     3901 cctgcttgat aaactcctgg ctggccacct tggtccagta ctgcatggcc tccttggcaa
     3961 agccgtagca atcgcccagc gtgtcgagac gctgctccac ccaggccacc gccgcatcga
     4021 agctgggctg gctgatcagc tcctcatgga gggcggcgcg cgccatatgg cccatgcccg
     4081 cggtggaggt gatcgtggcg atggcgccgc ccttgcggat cttcggagcc cactgctcgg
     4141 tccagtagcg catgccgatg tagctgacct tgatcacgtc catcggcggg aaggtctgcg
     4201 gcaggcccga gcagttgaac aggcagtcga tctcgccacc gatcgcggca acagcggcat
     4261 cgatcgaggc tggatcgcgc aggtcaacct gggtgaagga ggccaggttc actggcgacg
     4321 gcttgatgtc cacgccgtgc acttcggcgc caaggttcac cagctcgcgc gcagcggcct
     4381 cgcccatccc tgagaaacag ccggcgatca ccacccgctt gccttcgaac gaccacactc
     4441 ccattccacc ttctccatcc gttggcgttt tcggctttac ctcaaccggg ggcgaagcgt
     4501 gctcgtcccc ccttgactca ggccggctgc ggcgggactg taccgattag tttagtcgcc
     4561 gcaagaacca acgagaggtt agcagaaatg gccaccgccg ccacgaccgt ggacgtacct
     4621 ccccatgtca cccccgaccg cgtggtcgac ttcgatttct acaacgatcc gcgcttcgcg
     4681 gcggacgaca tccacaccgc cttcgccaag atgctcaagc aggtgccgcc ggtgttctgg
     4741 accccgcaca atggcggcca ctgggtgatc gccggccatg aggaactgtt cgaggcgtcg
     4801 cgcaacaccg agctgttctc cagcgccaag ggcggcctgc cgccttcgcc ttatgagccc
     4861 agccagctcc cgatcagcat cgatccgccc cggcacgcca tctatcgcga accgctggcc
     4921 cg
//
LOCUS       DATNBV010000262         3111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_802009,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000262 DATNBV010000000
VERSION     DATNBV010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802009"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VN627_06305"
     CDS             <1..983
                     /locus_tag="VN627_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HXD02903.1"
                     /translation="HLGNVFGSAAFVGWYNGHPQFADLNPDLSGSSAVVIGMGNVALD
                     VARILAKTRAEFAGSDIVGHALDQLDGSRLSTITILGRRGPHQIAMTPKELGELGHLE
                     RACPRVAPTDLPEEAEDFVLEPGLRKSVTLLRGFAGIPEAFREDKPVEIDFDFFASPR
                     AMIGEGGRVTGVDVERTVVEKGRAVGTGETYVIPASLVVCCIGYQTSPIPDVPFDERA
                     GRFANDEGRILAGLYCVGWARRGPTGTIGTNRPDGYAVIDLIAADQPHAAAKSGAAGF
                     DALAEARGLKVVRFRDWKKIEEAEQRAAREGAPREKFVDVERMIAAAGKD"
     gene            complement(1078..2196)
                     /gene="dnaN"
                     /locus_tag="VN627_06310"
     CDS             complement(1078..2196)
                     /gene="dnaN"
                     /locus_tag="VN627_06310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXD02904.1"
                     /translation="MKATIERATLLKCLSHVQSVVERRNTIPILSNVLIEAAPAGAVR
                     LMATDLDLQVVESMAAVSVEVPGAITVSAHLLFDIARKLPDGSQVSLETADNRMTVKA
                     GRSRFSLPTLPRDDFPVIVEGDLPTSFEIPARILAELIDRTRFAISTEETRYYLNGIF
                     FHVTDDELKAAATDGHRLARFTIPRPDGAEGMPDVIVPRKCVAELRKLLEEALDTNVL
                     VDLSASKVRFTLGGENGVVLTSKLIDGTFPDYTRVIPTGNDKLLRLDPRAFYEGVDRV
                     ATIATEKTRAVKIALEPDRVTLSVTSPDNGTAAEELPADYKSAGFEIGFNANYLKDIL
                     SQIDGDNVELHLADAGAPTLIRQGDAAAALYVLMPMRV"
     gene            complement(2379..>3111)
                     /gene="fabG"
                     /locus_tag="VN627_06315"
     CDS             complement(2379..>3111)
                     /gene="fabG"
                     /locus_tag="VN627_06315"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HXD02905.1"
                     /translation="TGASGGIGSSIARALASQGARLAISGSNATKLRAFRDELDEHTP
                     QHLQEVDHVAIACDLSDAEQVEKLVPAAVDSLGKLDILVNNAGITRDGLIMRMKDEDW
                     DQVMRVNLEAAFRLMRAAAKPMMKARFGRIITITSVVGTTGNPGQVNYAAAKGGLVAM
                     SKSLGQELASRNITVNCVAPGFIRTAMTDVLPDAQKEALNARIPMGRMGEGEDIGAAV
                     AYLASKEAGYVTGQTLHVNGGMAMI"
BASE COUNT          493 a          954 c         1126 g          538 t
ORIGIN      
        1 aacatctggg caatgtcttc ggcagcgcgg cctttgtcgg ctggtacaat gggcatccgc
       61 agttcgccga tctaaatccc gacctttcgg gttccagcgc cgtcgtcatc ggcatgggca
      121 atgtcgcgct cgacgtcgcg cggatcctcg ccaagacccg tgccgaattt gccgggagcg
      181 acatcgtcgg ccatgcgctt gaccagctcg atggctcacg cctgtcgacc atcacgatcc
      241 tcggccgacg tggcccgcac caaatcgcga tgacgccgaa ggaactgggc gaactgggcc
      301 atctggagcg cgcttgccct cgggttgccc cgaccgacct gccggaggaa gccgaggatt
      361 tcgtgctgga gccgggcctg cgtaagtcag tcaccctgct gcgcggcttt gccgggatcc
      421 ccgaggcctt ccgcgaggac aagccggtgg agatcgactt cgatttcttc gccagccccc
      481 gcgcaatgat tggcgagggc gggcgggtga cgggcgtcga cgtggagcgc accgtggtgg
      541 aaaaaggccg cgccgtgggc acgggggaga cctatgtgat ccccgccagc ctggtcgtct
      601 gctgcatcgg ctaccagacc tcgccgatcc ccgacgtgcc gttcgacgaa cgcgcgggcc
      661 gcttcgccaa cgacgagggg cggattctgg ccgggctcta ctgcgtgggc tgggcgcggc
      721 gcgggccgac cgggacgatc ggcaccaacc ggccggacgg ctatgcggtg atcgacctga
      781 ttgccgccga ccagccccat gcggcagcga aatcgggcgc tgcgggattc gatgcgctgg
      841 ccgaggcacg cggcctcaag gtggtgcgct tccgggattg gaagaagatc gaggaagcgg
      901 agcagcgcgc ggcgcgcgag ggggcgccgc gggagaagtt cgtggacgtg gagcggatga
      961 tcgcggcggc ggggaaggat taagggcggt acgggacagc acggcgcttg cctgagcgcc
     1021 cctccgtcag cctgcggctg cccacctccc gactgatggg aggagggggg ctttccctca
     1081 gacccgcatc ggcatcagga cgtagagcgc ggcggccgcg tcgccctggc ggatcagggt
     1141 gggggctccg gcgtcggcca ggtggagttc gacgttgtcg ccgtcgatct ggctgaggat
     1201 gtccttgaga taattggcgt tgaacccgat ctcgaagccg gcggacttgt aatcggccgg
     1261 gagttcctcg gcggccgtgc cgttgtccgg gctggtgacc gagagggtca cccggtcagg
     1321 ctcgagcgcg atcttgacgg cacgggtctt ttcggtggcg atcgtggcca cgcggtcaac
     1381 gccttcgtag aaggcccggg gatcgaggcg gaggagcttg tcgttgccgg tggggatcac
     1441 gcgcgtgtaa tcggggaagg tgccgtcgat cagcttggag gtgaggacca caccgttttc
     1501 gccgcccagc gtgaagcgca ccttggaggc cgagagatcg accagcacgt tggtatcgag
     1561 cgcctcttcc agcagcttgc gcagttcggc gacgcatttg cgcggcacga tcacatccgg
     1621 catgccctcc gcgccgtccg gacgaggaat ggtgaagcgg gcgaggcggt ggccgtcggt
     1681 tgcagcggcc ttcagttcgt catcggtgac atggaagaag atgccgttga ggtagtagcg
     1741 cgtttcctcg gtgctgatcg cgaagcgggt gcggtcgatc agttcggcga ggatgcgggc
     1801 cgggatctcg aacgaggtgg gcaggtcgcc ctccacgatc accgggaaat catcgcgcgg
     1861 gagcgtgggc agcgagaagc ggctgcgccc ggccttgacg gtcatccggt tgtcagccgt
     1921 ctcgaggctg acctgcgaac cgtcgggcag cttgcgggcg atgtcgaaca ggaggtgcgc
     1981 cgagacggtg attgcgccgg gcacttcgac cgagacggcg gccatggatt cgaccacctg
     2041 gagatcgagg tcggtcgcca tgaggcggac ggcgccggcg ggagcggcct cgatcagcac
     2101 gttcgagagg atcgggatcg tgttgcggcg ttcgacgacg gactggacgt gcgacaggca
     2161 cttgagcagc gtcgcgcgtt cgatagtggc cttcatgtcg ctctcatgtc cccctgggct
     2221 atccgcccgc gcggaatcgc ccgtttccgt caagaatttg gactgcctgt cttaacgcag
     2281 gctgccggtg cggcaagcgg gaaccatggc taacgcatgc gatctgggga taattcgcag
     2341 gaaacaggcc agttgcgcga gattcccgcg agccggcctc agatcatcgc catgccgccg
     2401 ttgacgtgca acgtctgccc ggtgacgtat cccgcttcct tgctggcgag gtaggccacc
     2461 gccgcgccga tatcttcgcc ctcacccatg cggcccatcg ggatgcgcgc gttgagcgcc
     2521 tccttctggg catccggcag cacatccgtc atcgccgtgc ggatgaagcc gggggcgacg
     2581 cagttgacgg tgatgttgcg gctggcgagt tcctggccga ggctcttcga catggcgacg
     2641 agcccgccct tggccgcggc gtagttcacc tggccgggat tgccggtggt gcccaccacc
     2701 gaggtgatcg tgatgatgcg gccgaagcgc gccttcatca tcggcttggc ggcggcgcgc
     2761 atcaaacgaa aggcggcctc gaggttgact cgcatgacct ggtcccagtc ctcgtccttc
     2821 atccgcatga tcaggccgtc gcgggtgatg ccggcgttgt tgaccaggat gtcgagcttg
     2881 ccgagggagt ccacggcggc ggggaccagc ttttccacct gctcggcatc ggacaggtcg
     2941 caggcaatgg cgacatggtc gacctcctgc aggtgctgcg gggtatgctc gtccagctca
     3001 tcgcgaaagg cgcgcagctt ggtggcattg gagccggaaa tagccagccg ggccccctgc
     3061 gaggccaagg cccgggcaat ggacgagccg atgccgccgg aggcgccggt g
//
LOCUS       DATNBV010000263         2651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4295547,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000263 DATNBV010000000
VERSION     DATNBV010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4295547"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VN627_06320"
     CDS             complement(<1..559)
                     /locus_tag="VN627_06320"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXD02906.1"
                     /translation="MKQRSYLAIEGVTVEFATKTGPFRALDHIDLSIEKGQFVSLIGH
                     SGCGKSTLLNAVAGLNKPTRGVIFLDGEVVEAPGPDRAVVFQDHSLLPWLTVAENVAL
                     AVGKTAQGKSKAEQRDWMMHTLDLVQMGHAATKRPGEISGGMKQRVGIARAIAMNPRV
                     LLMDEPFGALDALTRAALQDVVMDLQ"
     gene            complement(612..1568)
                     /gene="ntrB"
                     /locus_tag="VN627_06325"
     CDS             complement(612..1568)
                     /gene="ntrB"
                     /locus_tag="VN627_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HXD02907.1"
                     /translation="MAQMTVEIAENAPPLPESPPTLEQRGVLVEYPKSAIARRLLPPG
                     KAPSALHERLNRIAGAILPTAIMLTVLLVVWQLACGHESSALPGPLKVWQESHDVIMH
                     PLKGVTLDGAGLHITEEGDVGIAGHILRSLGRVLIGYSLSAVAGVMLGMLIGTNIYAY
                     RALDPLFQVLRTVPPLAWLPISLAIFQDARPSAIFLIFITAIWPIILNTAAGVQNIPA
                     SYRNVARVLALNPLEYFFRIVLPACVPHIFTGLRIGVGMSWLAIVAAEMVQGGTGIGF
                     FIWDSYNSSLLSDTIVALLWIGMVGFSLDRIVAAVGRLVART"
     gene            complement(1583..>2651)
                     /locus_tag="VN627_06330"
     CDS             complement(1583..>2651)
                     /locus_tag="VN627_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HXD02908.1"
                     /translation="KHGMPEVVVAKQASWGATRDNLVLGSGAGGIDGGHLLSPLPYFL
                     TMGIQARATPMNILARLNVNGQGISVDKEYMAAHADLNAAKMKGVIAQRKGAGKKITM
                     AMTFPGGTHDLWIRYWLAAGGINPDVDVNLITIPPPQMVANMKAGTMDAFCVGEPWNE
                     QLVQQQLGYSAVSTGQLWMNHPEKCFGMRADWVAAHPNAAVALTAAIIEAQRWCDSPA
                     NVSRLAMMIGGRDYLKVAVGDIASRLQGNFGMGDGRRYTNAPFKMKFFAGNASFPYKS
                     HDLWFITENQRWGKIPLSTNGLAVVNRVNRADIWRRAGASVGVAAPAGDSRGPERFFD
                     GKVFNPANPRAYLASLGIKRV"
BASE COUNT          439 a          832 c          935 g          445 t
ORIGIN      
        1 actggaggtc catgaccacg tcctggaggg cggcccgggt cagcgcatcg agcgcgccga
       61 acggttcgtc catgagcagg acgcgcgggt tcatcgcgat cgcccgggcg atgccgaccc
      121 gctgcttcat cccgccggag atttcccccg ggcgcttggt ggcggcatgg cccatctgga
      181 cgaggtcgag cgtgtgcatc atccagtcgc gctgctcggc cttgctcttg ccctgggcgg
      241 tcttgcccac ggccagggcg acgttttccg cgacggtcag ccagggcagg agcgagtgat
      301 cctggaagac cacggcccga tcaggccccg gcgcttcgac cacttcgcca tcgaggaaga
      361 tgacgccccg ggtgggcttg ttgagcccgg ccacggcgtt gagcagggtc gacttgccgc
      421 accccgagtg gccgatcagc gagacgaact gccctttctc gatcgagagg tcgatatgat
      481 cgagcgcgcg gaacgggcca gtcttggtcg cgaattcgac cgtgacacct tcgatcgcga
      541 gatagctgcg ttgcttcatg atgaattcct tctggccgaa agttcgggcc gaaagctctg
      601 gtgcaggcgg gtcaggtgcg ggcgaccagg cggcccacgg cggcgacgat gcggtcgagc
      661 gagaagccga ccatgccgat ccacaagagg gcgacgatcg tgtcggacag cagcgaggaa
      721 ttgtagctgt cccagatgaa gaagccgatg ccggtgccgc cctggaccat ttcggccgcg
      781 acgatggcca gccagctcat gccgacaccg atgcgaaggc cggtgaagat atgcggaacg
      841 caggccggca ggacgatgcg gaagaagtat tcgagcgggt tgagcgccag gacccgcgcg
      901 acattgcggt aggacgcggg gatgttctgc acgccggcgg ccgtgttgag gatgattggc
      961 cagatcgcgg tgatgaagat caggaagatc gccgaagggc gcgcgtcctg gaagatggcg
     1021 aggctgatcg gcagccaggc cagcggcggc acggtgcgca ggacctggaa cagcggatcg
     1081 agcgcgcggt aggcatagat attcgtgccg atcagcatgc ccagcatcac cccggcgacg
     1141 gccgacagcg aatagccgat caggacccgg ccaaggctgc gcaggatgtg gccggcaatg
     1201 ccgacatcgc cttcttcggt gatgtgcagc cccgcgccgt cgagcgtgac gcctttcagc
     1261 gggtgcatga tcacgtcgtg gctttcctgc cagaccttga gcgggcccgg cagggccgag
     1321 ctctcatgac cgcaggccag ctgccagacg accagcagga ccgtcagcat gatcgcggtg
     1381 ggcaggatcg cgccggcgat ccggttgagc cgctcgtgca aggcgctggg cgccttgccg
     1441 gggggcagca gccggcgcgc gatggccgac ttggggtatt cgacgaggac gccgcgctgc
     1501 tccagcgtcg gaggcgattc tgggaggggc ggcgcgtttt ccgcgatctc tacggtcatc
     1561 tgggccatgg tcgtcgccct cctcaaaccc gcttgatgcc aagggaagcg aggtaggctc
     1621 gcgggttggc ggggttgaac accttgccgt cgaagaagcg ttccggcccg cggctgtcgc
     1681 ccgccggggc ggccacgcca accgaggccc cggcccggcg ccagatatcg gcccggttga
     1741 cccggttgac cacggccaag ccattggtgc tgagcggaat cttgccccag cgctggttct
     1801 cggtgatgaa ccagagatcg tggctcttgt aggggaacga ggcgttgccc gcgaagaact
     1861 tcatcttgaa gggagcattg gtgtagcggc gcccgtcgcc catgccgaaa ttgccctgca
     1921 accggctggc gatatcgccc acggccacct tcagatagtc acgcccgccg atcatcatgg
     1981 ccagccgcga gacgttggcg gggctgtcgc accagcgctg ggcctcgatg atcgcggcgg
     2041 tcagggccac ggcggcgttc ggatgggcag caacccagtc ggctcgcatg ccgaagcact
     2101 tttcggggtg gttcatccac agctgcccgg tcgacaccgc ggaatagccg agctgctgct
     2161 ggaccagctg ttcgttccac ggctcgccca cgcagaacgc gtccatcgtg ccggccttca
     2221 tgttggcgac catctgcggc ggcgggatgg tgatcaggtt cacgtcgaca tcgggattga
     2281 tccccccggc ggccagccag taacggatcc acaggtcgtg ggtgccgccg gggaaggtca
     2341 tggccatggt gatcttcttg cccgcgccct tccgctgggc gatcacgccc ttcatcttgg
     2401 cggcgttgag atcggcgtga gccgccatgt attccttgtc gaccgagatg ccctgcccgt
     2461 tgacgtttag gcgggcgagg atgttcatcg gcgtcgcgcg ggcctggatg cccatggtca
     2521 gaaagtaggg cagcgggctg agcaggtggc cgccgtcgat gccgccggcg ccgctgccca
     2581 ggaccaggtt gtcacgcgtg gcgccccagc tggcctgctt ggcgacgacc acctcgggca
     2641 tgccgtgctt g
//
LOCUS       DATNBV010000264         3826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_465705,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000264 DATNBV010000000
VERSION     DATNBV010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465705"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VN627_06335"
     CDS             complement(<1..407)
                     /locus_tag="VN627_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02909.1"
                     /translation="MAEAQSAGTGAGKLVIRNIGLLLSGDLENPILDADTIVAVDGKI
                     TAIGKAKDVDTEKTTTIIDAKGTTLAPGLIDSHVHPVFGDWTPRQSQLNWIDSFLHGG
                     VTTMVSAGEVHLPGRPKDVVGVKALAITAQRAF"
     gene            complement(510..1262)
                     /locus_tag="VN627_06340"
     CDS             complement(510..1262)
                     /locus_tag="VN627_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HXD02910.1"
                     /translation="MIELDKVVDALSHHMPIKLGVLRLADGPVPGLRTGLVIVDEVNG
                     FATVGAGYLAPPVENPQVTQMIAETNRLAREFTSRKWPIFAFLDSHTPGKAEPPYPPH
                     CEIGTGEENLVPDLLWLESDPHTTLVRKDCINGFVGAIAPDGRNLVSEWVNGHKLEQI
                     LVVGVCTDICVMDFVLTLLSARNHDLMPSLRDIMVYEGACATYHLPRVTAESLGLGAH
                     AAHPQDVAHYMGLYFMSSRGAQLVDKVEIAAG"
     gene            1516..2640
                     /locus_tag="VN627_06345"
     CDS             1516..2640
                     /locus_tag="VN627_06345"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXD02911.1"
                     /translation="MPIPFVDLQAQRRRLGDSVEEGMRRVLTHGQFVMGPEVGQLEKA
                     LSEFCGVPHVIACANGTDALNLAMRALGIGAGDAVIVPAFSFSASVEAVLLLGATPVF
                     CDVLAESCNIDIASMAGAVAAARAAGLNPRAVMPVDLFGLPADYATLDAAAASLGLAI
                     VADAAQSFGASRGNRQVGTFGNIAATSFFPSKPLGCYGDGGAVFTADAEAAALLRSLR
                     QHGEGRDRYDTVRIGINSRLDSLQAAVLLAKLSIFAEEIELRQKVAERYSAALADVVE
                     VPVIDRKARSVWAQYTVKVDNRNSVAAGLKAVGVPTAIYYPRSLNHQPAYRGYPTVAG
                     GVPVAEALAQRVLSLPCHPYLDMATQDYVIDQLRAVIAAA"
     gene            complement(2675..3145)
                     /locus_tag="VN627_06350"
     CDS             complement(2675..3145)
                     /locus_tag="VN627_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXD02912.1"
                     /translation="MINAKNILAVVCRLCERVAVMPHGRLVEIGTTDQILTAPRGLYP
                     GAAELFREVDVLLTPTCTVPPVPLDAHDLDAPRATVKDLFDHLAPIETFTALFNATGQ
                     PAMSLPLCWSKSGLPLGMQLVGRGGDEATLFRVAAALEQALPWHERRPPIHVAA"
     gene            3285..3491
                     /locus_tag="VN627_06355"
     CDS             3285..3491
                     /locus_tag="VN627_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02913.1"
                     /translation="MTLPQIAGQQFDAAGMHERIAGQQLVIAGDDTSTAEAVDRVDRL
                     TVDLALELFGLSGQPPRLVPLVAA"
     gene            3510..>3826
                     /locus_tag="VN627_06360"
     CDS             3510..>3826
                     /locus_tag="VN627_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02914.1"
                     /translation="MHPPIFHRRFDYDRSLLPNAFLAQLEEGVTSIDEARLRSGASIG
                     YPGWGAIYYLALCALDPSRPATIIETGANWGCSTIVLASALRAAKCGGHVHTIEIDPV
                     NAE"
BASE COUNT          629 a         1258 c         1263 g          676 t
ORIGIN      
        1 tcgaaggcgc gctgcgcggt gatcgccagc gccttgacgc cgacgacatc cttgggccgg
       61 cccggcagat gcacctcgcc ggccgagacc atggtggtga cgccgccatg gaggaagctg
      121 tcgatccagt tgagctggct ctggcgcggc gtccaatcgc cgaagacggg atggacatgg
      181 ctgtcgatca gcccgggggc gagagtcgtg cccttggcgt cgatgattgt ggtcgtcttc
      241 tcggtgtcca catcctttgc cttgccgatg gccgtgatct tgccgtcgac ggcgacgatg
      301 gtatcggcgt cgagaatggg attctcgaga tcgcccgaga gcaggagccc gatattgcgg
      361 atcacgagct tgccggcgcc ggtgccggca gattgtgctt cagccatgtc agatgttcct
      421 tggaagggta ttcggtccgg ttgtcacaac gtcgctctct atccccatcg tcattcccgc
      481 tttcgcgggg atgacggaag ggacggtatc tagcccgcgg cgatctcgac cttgtcgacg
      541 agctgcgcgc cgcgggagga catgaaatag aggcccatgt aatgggcgac gtcctggggg
      601 tgggcggcat gggcgccgag gcccaggctc tccgccgtca cccgcggcag atgataggtg
      661 gcgcaggccc cctcatagac catgatatcc ctgaggctcg gcatcagatc gtggttgcgg
      721 gccgagagca gggtcagaac gaaatccatc acgcagatat cggtacagac gcccaccacc
      781 aggatctgct ccagcttgtg cccgttcacc cattcgctga cgagattgcg cccgtccggc
      841 gcgatggccc cgacgaagcc gttgatgcag tccttgcgca ccagggtcgt atggggatcg
      901 ctctccagcc agagcaggtc gggcaccaga ttctcctcgc cggtgccgat ctcgcaatgg
      961 ggcgggtagg ggggctcggc tttgccgggc gtgtggctgt cgaggaaagc gaagatcggc
     1021 catttgcggc tggtgaattc ccgcgccagg cgattggtct ccgcgatcat ctgagtcacc
     1081 tgcggattct ccaccggcgg ggccaaatag ccggcgccca ccgtggcgaa gccgttcacc
     1141 tcatcgacga tgacgagccc cgtgcgcagg cctggcaccg gcccatcggc cagccgcagc
     1201 accccgagct tgatcggcat gtggtggctg agggcgtcga cgaccttgtc gagctcgatc
     1261 atagccggcg tcttatcccc tcctggcagc ggcgagcctg gcctcgcctt cggtctcgcc
     1321 aatattgctc cgccacagcc ttccgaggca agttggagcg ctttcggccg gctttcgggg
     1381 cgccgggcag cggcccccgc gacgaaatga ggcctgcaat ttgcgattcg ctgtgctaag
     1441 cagccttgaa tcactcggac acatcccccc ggaaccggtg ttatgaacga catgtcgaag
     1501 cccgctgctg tcaaaatgcc tattcctttc gtggacttac aggcgcagcg ccgccggctg
     1561 ggcgattcgg tggaagaggg catgcgccgc gtcctgaccc atggccagtt cgtcatgggc
     1621 cccgaagtgg gccagctgga gaaggcgctc tccgaattct gcggcgttcc ccatgtgatc
     1681 gcctgtgcca atgggaccga cgccctgaac ctcgccatgc gggcgctggg gatcggggcg
     1741 ggggatgcgg tgatcgtccc ggccttcagc ttctccgcct cggtggaggc ggtgctgctg
     1801 ctgggtgcca cgccggtgtt ctgcgacgtg ctggccgaga gctgcaatat cgacatcgcc
     1861 agcatggccg gggccgtcgc ggccgcgcgc gcggcagggc tcaatcctcg tgccgtcatg
     1921 cccgtcgacc tcttcggcct gccggcggat tacgccaccc tcgacgccgc ggcggcgagc
     1981 ctcggcctgg cgatcgtcgc cgatgcggcc cagagcttcg gcgccagccg cggcaatcgg
     2041 caggtcggca ccttcggcaa tatcgcggcc accagcttct ttccctccaa gccgctcggc
     2101 tgctacgggg acggcggcgc cgtattcacc gccgatgcgg aggccgccgc gctgctgcgt
     2161 tcgctgcgcc agcatggcga gggccgcgac cgttatgaca cggtgcgcat cggcatcaat
     2221 tcgcggctgg acagcctgca ggccgcggtg ctgcttgcca agctctccat cttcgccgag
     2281 gagatcgagc tgcgccagaa ggtcgcggaa cgctacagcg ccgctctggc cgatgtcgtt
     2341 gaagtgccgg tgatcgaccg caaggcgcgc tcagtctggg cccaatacac cgtcaaggtc
     2401 gacaatcgca atagcgtggc ggcggggctg aaggccgtgg gcgtgccgac cgccatctat
     2461 tacccgcgca gcctcaatca ccagccagct tatcgcggct atccgacggt ggccggcggc
     2521 gtgcccgtgg cggaagccct cgcccagcgc gtgctgagcc tgccctgcca cccctatctc
     2581 gacatggcga cccaggatta cgtcatcgat cagcttcgcg cggtcatcgc cgcggcctag
     2641 ccgccggcga acccgtcagc ctctgacgtc cctgctacgc cgccacatgg atcggcgggc
     2701 gccgctcgtg ccacggcaag gcctgctcca gcgccgcagc cacacgaaac agcgtggcct
     2761 catcgccgcc gcggccgaca agctgcatgc cgagcggcaa tcccgatttg ctccagcaga
     2821 gcggcaatga catggccggc tggcccgtcg cgttgaacag cgcggtgaag gtctcgatcg
     2881 gcgccagatg gtcgaacagg tccttgaccg tggcccgcgg tgcgtcgagg tcatgggcat
     2941 cgagcggcac gggaggaacg gtgcaggtag gggtgagcag aacatcgacc tcccggaaga
     3001 gttctgcagc tcctgggtat aggcctcgcg gggcagtgag tatctggtcc gtggtcccga
     3061 tctccaccag ccggccatgg ggcatcacgg ccacgcgctc gcagagacgg cacaccacgg
     3121 ccaggatgtt ttttgcgttg atcatgctcc ggccgccccg agcttgccgc agtgctgagg
     3181 ctatgttagc aagcaaacga gatgaacgtg ggttcacgta gccgagagtc gtcatacgta
     3241 actaccctcg ttttgcagcg gagcccgaag cccatggcat caaaatgacg ctgccgcaga
     3301 ttgccgggca gcagtttgat gccgccggca tgcacgagag gatcgccggc cagcagctcg
     3361 tcatcgccgg cgacgacacc agcaccgccg aagcggtcga ccgcgtcgac cggctcaccg
     3421 tcgatctcgc gctggagctg ttcggcctct ccggccagcc gccacggctg gtgccgctgg
     3481 tggcggcgta gcggaaggag accggagtct tgcacccgcc catattccat cgccggttcg
     3541 actatgaccg ctcgttgctg ccgaatgcct tcctagccca gttggaggag ggcgtaacgt
     3601 cgatcgacga ggctcgcctg cgtagcggcg catcgatcgg atacccggga tggggggcga
     3661 tctactacct ggcgctgtgc gctctcgacc cctcgaggcc tgcaacaatc atagaaaccg
     3721 gagcgaactg gggctgctcc accatcgttc tggcaagtgc tttgcgcgcc gcgaaatgcg
     3781 gcgggcacgt ccacaccatt gagatcgacc cggtcaatgc cgagaa
//
LOCUS       DATNBV010000265         2909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_803257,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000265 DATNBV010000000
VERSION     DATNBV010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803257"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VN627_06365"
     CDS             <1..700
                     /locus_tag="VN627_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXD02915.1"
                     /translation="AMLMRVAVMAFGGQAGAWQQIVIFVAVASIVIGALGAIGQQNIK
                     RLLAYSSINNVGFILIGLAVATQQGASAMLTYLAIYVAMTVASFVVVLLLRDEAGNPV
                     EGFADIAGLSRTRPWLAASMAIVMFSLAGIPPLFGFWGKFVVFQAAVAAGFVPLAAIG
                     IAASVIGAFYYLKVVKVMYFDEPAGRVTGTSDLAHNAILVATSLFLSPVGYLLTKWLG
                     GMTDTAAAALFHVA"
     gene            697..1419
                     /locus_tag="VN627_06370"
     CDS             697..1419
                     /locus_tag="VN627_06370"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HXD02916.1"
                     /translation="MIDFVPETGSTNGDLVARLAGGANVPENYWLVADRQTAGRGRQG
                     RAWFDGTGNFMGSTVVHARVGDPALPSLALVAGLAAHSVLSGMVPAPRRPELKWPNDV
                     LVGGAKLAGILLESAGNGVVVGIGVNLASAPEVPGRQTVALGEFGPAPDRDAFAHGLA
                     AVFAAELDLWRSAGLAALITRWTAAAHPIGTSLLVGEPGEEPLAGRFAGLDATGALIL
                     TLGDGTKRTIQAGEVRLAPAAE"
     gene            1431..2225
                     /locus_tag="VN627_06375"
     CDS             1431..2225
                     /locus_tag="VN627_06375"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HXD02917.1"
                     /translation="MLLAIDLGNTNVVFALIDLGAPEGPSIRTRWRIATDPRRTGDEY
                     AVWLSQLLALEGLSRDAIDQIIISTVVPRALHNMKVLADKYFGIDPLIAGEGTAGYGI
                     DLDIDQPASLGADRAVNAVAAHAQYGGDLIVVDFGTATTFDVVDFNGAYKGGIIAPGI
                     NLSLDALVGNTAKLPRIAIEAPKSASVIGTNTEDQMLIGVFWGYVAMMEGLIARMKAE
                     IGRPAKVIATGGLAVLFDQHTTIFDAVDADLTLNGLALLGQRARRK"
     gene            2263..>2909
                     /locus_tag="VN627_06380"
     CDS             2263..>2909
                     /locus_tag="VN627_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HXD02918.1"
                     /translation="MTPGKELLFCALGGSGEIGMNVNLYGCDGKWLMVDLGMTFSGNE
                     YPGVDLVFADLEFIERERENLIGVVLTHAHEDHIGAVPYFAEDLGVPLYATPFTARLV
                     RAKLEEAGIASRVQLNVVDDLEQFQLGPFSIRYVPLAHSIAEGNALLIGTPYGRIFHT
                     GDWKLDDEPLIGEPATAEELTALGDQGVLALVCDSTNVFNPEASGSEGEVRRGLLA"
BASE COUNT          463 a          912 c          983 g          551 t
ORIGIN      
        1 ggccatgctg atgcgcgtgg cggtgatggc cttcggcggg caggcgggcg cctggcagca
       61 gatcgtgatc ttcgtcgcgg tcgcctcgat cgtgatcggc gcgctcggcg cgatcgggca
      121 gcagaacatc aagcgcctgc tcgcctattc gtcgatcaac aacgtcggct tcatcctgat
      181 cgggctggcc gtggcgacgc agcagggcgc ctcggccatg ctgacctacc tggcgatcta
      241 cgtggcgatg accgtggcga gcttcgtcgt ggtcctcctg ctgcgcgacg aggcgggcaa
      301 tccggtggag ggctttgccg acatcgccgg cctttcccgc acccggccgt ggctggcggc
      361 gagcatggcg atcgtgatgt tcagcctggc cggcattccg ccgctgttcg gcttctgggg
      421 caagttcgtg gtgttccagg ccgcggtagc cgccggtttc gtgccgctcg ccgccatcgg
      481 tatcgccgcc tcggtgatcg gcgccttcta ctacctgaag gtggtcaagg tcatgtactt
      541 cgacgagccg gcgggccggg taaccggcac gagcgatctt gcccataacg cgatcctggt
      601 tgccaccagc ctgttccttt ccccggtcgg ttacctgctg accaagtggc tgggcggtat
      661 gaccgatacg gcggctgcgg ccctgttcca cgtcgcttga tcgacttcgt tcccgagacc
      721 ggctcgacca acggcgatct tgtcgcccgt ctggcgggcg gcgcaaacgt tcccgagaac
      781 tactggctgg ttgccgatcg gcagacagcc gggcgcggac ggcagggccg ggcgtggttc
      841 gacggtaccg gtaatttcat gggctcgacc gtggtccacg cccgcgttgg cgatccggcc
      901 ttgccgagcc tggcgctggt tgccgggttg gcggcgcaca gcgttctctc cggcatggtt
      961 cccgcgccgc gacgccctga gctcaaatgg cccaatgatg tgctggtcgg cggggcgaag
     1021 cttgcgggga tcctgctcga gagcgcgggc aatggcgtgg tggtcggcat cggcgtcaac
     1081 ctggcctcgg cgcccgaagt accgggtcgt caaactgtcg cgctcggcga attcggtccc
     1141 gcgccggatc gcgatgcttt tgcgcatggc ctcgccgccg tctttgccgc agaacttgac
     1201 ctgtggcgca gcgcgggcct ggccgcgttg atcacccgat ggactgcagc ggcccacccg
     1261 atcgggacct cgctgctcgt cggtgagcct ggcgaggagc ctctggcagg ccgtttcgcc
     1321 ggccttgatg ccacaggagc cttgatcctg acgcttggcg acggcaccaa gcgcacgatc
     1381 caggcgggcg aggtgcggct cgcacctgcc gccgaatagg gaagcgaacc atgctgctgg
     1441 cgatcgatct cggaaacacc aacgtcgtct tcgcgctgat cgacctcggt gcgccggagg
     1501 gcccgtcgat ccgaacccgc tggcgtatcg ccacggaccc gcgccgcaca ggggacgaat
     1561 atgccgtgtg gctctcgcag ctcctggcgt tggaagggct gtcgcgcgat gcgatcgacc
     1621 agatcatcat ttccaccgtg gtgccacgcg cactgcacaa catgaaggtc ctcgcggaca
     1681 agtatttcgg tatcgatccc ctgatcgcgg gggaggggac ggcaggctat gggatcgatc
     1741 tggacatcga ccagcccgcc agccttggcg ctgaccgcgc ggtgaatgcc gtggccgctc
     1801 acgcccagta cggcggggac ctgatcgtgg tcgatttcgg cacggcgacc accttcgacg
     1861 tggttgactt caatggggcc tacaagggcg gcatcatcgc gcctggcatc aatctctcgc
     1921 tcgatgcgct ggtcgggaac acggccaagc tgccgcggat tgccatcgag gcgccgaaga
     1981 gcgccagcgt cattggcacc aacaccgagg accagatgct gatcggtgta ttctggggct
     2041 acgtggccat gatggaaggc cttatagctc ggatgaaggc cgagatcggt cggcctgcca
     2101 aggtaattgc cacaggcggt ctggcggtgc tgttcgatca gcacacgacg atcttcgacg
     2161 cggtggatgc cgacctcacg ctgaatgggc tggcactgct tggccaaaga gcgcgccgga
     2221 aatgagaaaa ggtttagctt ggatgacagg agatttgatc aagtgactcc cggcaaggag
     2281 ctcctgttct gtgcgctcgg cggatcgggc gagatcggca tgaacgtcaa cctctacgga
     2341 tgtgacggca agtggctgat ggtcgacctt ggcatgacct tcagtggtaa tgaatatccc
     2401 ggcgtcgacc tggtgtttgc cgacctggaa ttcatcgaac gagagcgaga gaacctgatc
     2461 ggcgtggtgc tgacccatgc ccacgaagat catatcggcg ctgtccccta tttcgccgag
     2521 gacctgggcg ttccgcttta cgccacgccc tttaccgccc ggctggtgcg ggcgaagctg
     2581 gaggaggcgg ggatcgcctc gcgcgtgcaa ctcaacgtgg tcgatgacct cgagcaattc
     2641 cagcttggtc ccttcagcat ccgttacgtt ccgctcgcgc actccattgc cgagggcaat
     2701 gcgctgctca tcgggacgcc ctacggtcgc attttccaca cgggcgactg gaagctcgac
     2761 gacgagccac tgatcgggga accggccacc gcagaggaac tgaccgcctt gggcgaccag
     2821 ggcgtgctgg cgctcgtctg cgattcgacc aatgtgttca accccgaggc gtcaggctcg
     2881 gaaggggagg tgcggcgcgg gctgctggc
//
LOCUS       DATNBV010000266         8975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_804951,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000266 DATNBV010000000
VERSION     DATNBV010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804951"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VN627_06385"
     CDS             <1..705
                     /locus_tag="VN627_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HXD02919.1"
                     /translation="EELYEEQQFEYQPLPQIVIIVDELADLMVTVGKEIEVLIQRLSQ
                     KSRAAGIHLIMATQRPSVDVITGVIKANLPTRISFAVTSRIDSRTILGEQGAEQLLGK
                     GDMLYKPATDPIKRVHGPFVSDEEVEKIADHWRGQGEPDYVDSVTEEPEDAPFGFDDL
                     EGGSDAPEDRKYRQVCQLVFESQKASASWIQRQMGVGYNTASKWIERMEQDGLVGPAN
                     HVGRREIYRDKDGNVL"
     gene            813..1256
                     /locus_tag="VN627_06390"
     CDS             813..1256
                     /locus_tag="VN627_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02920.1"
                     /translation="MKALTQCWFTRMHIPEGRAHKEDDGSHTSWCRHCERPIVSWDKA
                     RWFLADGFNVTHLAETVGGRFLFVVDSLEEAMIARYPVRDLPDEAAIEALKAELRERH
                     GIGKPGSTLELHDSGAKPAERRKLADKRARRVAPTGRSSPLSASY"
     gene            complement(1258..1659)
                     /locus_tag="VN627_06395"
     CDS             complement(1258..1659)
                     /locus_tag="VN627_06395"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HXD02921.1"
                     /translation="MSAPFTQTFAAQTEDIDVLGHVNNVVWVKWMEAIATAHWEAVSP
                     PEHKARYIWMVTRHEVDYRGNIREGETVTAETEVGEAPKGAQMTRAIRFRNAAGKVIV
                     NARTTWALIGQASGRLVRVPPEVAAPFYRAE"
     gene            complement(1656..3749)
                     /locus_tag="VN627_06400"
     CDS             complement(1656..3749)
                     /locus_tag="VN627_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXD02922.1"
                     /translation="MPDNTPPNTPPVAAKKPHSFTHHGITVADDYAWLRDPGYPEVKD
                     PEILAHLEAENAWFEARMAERKPLVETLFTEMRARIKEADKSVPQKDGDWLYWIEYEE
                     GAEYKKWWRRPVSAKDDPSADELILDEVALAEGHEYFRLGAISVSKDGKLLAWSVDDN
                     GSERFTARIKVLETGEILPDTIEGTLSGLVWVAGDKALVYSLANEQWRTDNARLHWLG
                     RPVSEDVELYHEDDEGFRVGSGISANEEWLVVSTSDHETSEVRLIPAADPLAAPLLVK
                     ERKAGIEYDVDVRGETLFIHANDTHENFRLATAPLAAPGEWTTLIEGTDAFYLTGFEL
                     FKEFYVIEGRRAGLDQIELRWYDDPARVEPIAFPEASYDAGLGDNPEWDTAVLRLGYE
                     SMVSPATTYDYHVADRRLEVRKVQEIPSGYDASLYVTERLEIPARDGTKIPVSIVSRK
                     DRDQMNGGAGGPLHLYGYGAYGIAIAPGFSTTRLSLVDRGFAYAIAHIRGGDDLGRAW
                     YKAGKLERRTNTFHDFVDVARALAAMGYTAPGKISISGGSAGGELMGAVMNEAPELWG
                     AVVAHVPFVDVLATMLDESLPLTPGEWPEWGNPIEDKAAFELIRSYSPYDQVEGKAYP
                     PLLVTAGLNDPRVTYWEPAKWVARLRELKTDSNELLLKTNMGAGHGGKSGRFESLRET
                     AEEFAFILWQLGLAG"
     gene            3810..5630
                     /locus_tag="VN627_06405"
     CDS             3810..5630
                     /locus_tag="VN627_06405"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HXD02923.1"
                     /translation="MSVAEARLDALRKELAARGLDGFVVPISDEHMSEYIGAYAQRLA
                     WLTGFRGSAGTAVVLRDEAAIFVDGRYTLQVRDEVDGKLYGYESVPQTSVAKWLGEHA
                     RQGMKVGYDAWLHGKRWADAAAKALAERGAELVAVDGNPVDAVWADRPAPSPAPALVH
                     PEDLAGQSSADKRAAVAAWLKAKGCAAAVISALDSVAWLLNIRGSDVERTPVALSFAI
                     ARADGSAELFIAPEKVTDDLRAHLGNAVSIRPRDNFAAALAELAGQQVAVDPERTVMG
                     VFVALEAAGASIVEAQDPCVLPKATKNAAEIAGHRAAQARDGAAMVHFLHWLSQEAPK
                     GTVDELAAADWLQRARAASGSLRDLSFDTISGSGPNGAIVHYRVSGETNRPLEPGSMY
                     LVDSGGQYPDGTTDITRTVWIGPGEPGAEERDRFTRVLKGNIALSRAVFPKGTLGSQL
                     DTLARQFLWHAGIDYAHGTGHGVGAFLSVHEGPQRISRATGGQGGTEQELLPGMILSN
                     EPGYYKAGAWGIRIENLLLVEERAIEGAESEFLGFDTLTHVPIDRALLNPAILEPGEV
                     AWWNAYHARVLEIVGPQVDGVVLEWLREQCAPLGMAGVQG"
     gene            complement(5752..6363)
                     /locus_tag="VN627_06410"
     CDS             complement(5752..6363)
                     /locus_tag="VN627_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HXD02924.1"
                     /translation="MDRRSLLTGAVAAGAMAALPGRAFAQAFSVSEQHRKILAIAAEQ
                     VERNRSRLWRADMAAVADFALPSALPRFHFANLEQGSVRSFLVAHGRGSDPEHDGFLK
                     IFSNVPNSYATSRGAYVTAEWYKGKYGTSIRLMGLDQDNSNVLERAIVLHPAWYANED
                     MLPKWGKLGRSDGCFAMGEADFNEALWHLSGGRLIYADKLDLG"
     gene            complement(6410..6931)
                     /locus_tag="VN627_06415"
     CDS             complement(6410..6931)
                     /locus_tag="VN627_06415"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HXD02925.1"
                     /translation="MKGFVIMAVGLVLALMLVGQFLSPVHPLSGPTGGVVIAAHAPAP
                     AEARFTPEGLVIDRDASGQFHLDVALNGQPTKFLVDTGADAVALTIADAERAGIKVDQ
                     SAFQPILQTASGQGWGTLITIDRLELGKTELHNVGAVVVKDLGVSLLGQSVLAQLGKV
                     ELKGDKMVLTPPN"
     gene            complement(6962..8101)
                     /gene="ispG"
                     /locus_tag="VN627_06420"
                     /gene_synonym="gcpE"
     CDS             complement(6962..8101)
                     /gene="ispG"
                     /locus_tag="VN627_06420"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HXD02926.1"
                     /translation="MSSIRPWRTIVRRKSRQIMVGKVPVGGDAPITVQTMTNTLTSDA
                     GATISQIRRCEDAGADIIRVSCPDEASTAAFREIARAAAVPLVADIHFHYKRALEAAD
                     AGAACLRINPGNIGSSDRVAEVVRAAKANGCSIRIGVNAGSLEKDLLEKYGEPCPEAL
                     VESALDHIKLLQDHDFHEYKVAVKASDVFLAVAAYQGLSEAVDCPLHLGITEAGGLIG
                     GTVKSSIGIGSLLWAGIGDTIRVSLSAEPEEEVRVGFEILKALDLRTRGVRIVSCPSC
                     ARQGFDVIRTVEALEKRLSHIKTPLSLSVLGCVVNGPGEARETDIGLTGGGNGKHMVY
                     LSGVTDHHVQSEAMLDHIVSLVEAKAAQLEAETTDAGKAEPQAAE"
     gene            complement(8171..8425)
                     /locus_tag="VN627_06425"
     CDS             complement(8171..8425)
                     /locus_tag="VN627_06425"
                     /inference="COORDINATES: protein motif:HMM:NF039182.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HXD02927.1"
                     /translation="MTDPKAMIQTMIALASASLGLVAALAWNEAIKATLAKLGMGEDL
                     AGLYSYAILATVLAVVVLTVLGRVSAKIGGEAAFEREAEG"
     gene            complement(8506..8751)
                     /locus_tag="VN627_06430"
     CDS             complement(8506..8751)
                     /locus_tag="VN627_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXD02928.1"
                     /translation="MELKQLYTAPNAQGAGIGAALMDWAMAEFAALGADEVQLSVWRH
                     NPGAQRFYRRYGFDQVAEVSFRVGEQLDEEFLFARML"
BASE COUNT         1414 a         2768 c         3302 g         1491 t
ORIGIN      
        1 gaggagctct acgaggagca gcagttcgaa tatcagccgc tgccgcagat cgtcatcatc
       61 gtcgacgaac tggccgacct catggtcaca gtcggcaagg agatcgaggt gctgatccag
      121 cggctctcgc agaagagccg cgcggctggc atccacctga tcatggcgac gcagcgcccc
      181 tcggtcgacg tcatcaccgg cgtgatcaag gccaacctgc cgacccgcat ctcgttcgcc
      241 gtcaccagcc ggatcgacag ccgcacgatc ctgggcgagc agggcgccga gcagctgctg
      301 ggcaagggcg acatgctcta caagccggcg accgacccga tcaagcgcgt ccacgggccc
      361 ttcgtcagcg acgaggaggt cgagaagatc gccgatcact ggcgcgggca gggcgagccg
      421 gactacgtcg attcggtcac cgaggaaccg gaggacgcgc cgttcgggtt cgacgacctg
      481 gaaggcggca gcgacgcgcc ggaagaccgc aagtaccggc aggtctgcca gctggtgttt
      541 gagagccaga aggccagcgc gagctggatc cagcggcaga tgggcgtggg ctacaacacc
      601 gcctccaagt ggatcgagcg gatggagcag gacgggctgg tcggcccggc gaaccatgtg
      661 ggccgccgcg agatctaccg ggataaggac gggaacgtgt tgtgaggcgc aagggcaagc
      721 ttcagcttgc ccggtccatg caccctgact cttccttaat tccccccgca atccgccgtt
      781 ttccctcccg gtagccacgg gatttgcaga cgatgaaggc tctcacccag tgctggttca
      841 cccggatgca tatcccggag ggccgcgcgc acaaggagga tgacggctcc cacacctcgt
      901 ggtgccggca ctgcgagcgg ccgatcgtct catgggacaa ggcccgctgg ttcctggccg
      961 atggcttcaa cgtgacccac ctcgccgaga cagtgggcgg gcgcttcctg ttcgtggtcg
     1021 acagcctgga ggaggcgatg atcgcccgct atccggtgcg ggacctgccc gacgaggccg
     1081 cgatcgaggc gctgaaggcc gaactgcgcg aacgccacgg gatcggcaag ccgggttcga
     1141 cgctggaact ccatgacagc ggcgccaagc cggcggagcg gcgcaagctg gccgacaaac
     1201 gggcgcggcg ggtggcgccc acgggacgca gctcgccgct ttcggcttct tactgagcta
     1261 ctccgcccgg tagaacggcg ccgccacctc cggcggcacg cgcaccagcc ggccgctggc
     1321 ctggccgatc agggcccagg tggtgcgggc gttgacgatg accttgccgg cggcgttgcg
     1381 gaagcggatg gcgcgggtca tctgggcgcc cttgggggct tcgccgacct cggtttcggc
     1441 ggtgacggtt tcgccctcac ggatgttgcc gcggtagtcg acctcgtggc gggtcaccat
     1501 ccagatgtag cgagccttgt gctcgggcgg ggagacggcc tcccagtgcg cggtggcgat
     1561 ggcttccatc cacttcaccc agacgacgtt gttcacgtgg ccgaggacgt cgatgtcttc
     1621 ggtctgcgcg gcgaaggtct gggtgaaggg ggcgctcatc cggcgagccc cagttgccag
     1681 aggatgaagg cgaactcctc ggccgtctcg cgcagggact cgaaccggcc cgacttgccg
     1741 ccgtggcccg cgcccatgtt ggtcttgagc agaagctcgt tggagtcggt cttgagttcg
     1801 cgcaggcggg cgacccactt ggccggttcc cagtaggtga cgcgcgggtc gttgaggccg
     1861 gcggtgacga ggagcggcgg gtaggccttg ccttcgacct ggtcgtaggg gctgtaggag
     1921 cggatcagtt cgaaggcggc cttgtcctcg atcgggttgc cccattcggg ccattcgccg
     1981 ggggtaaggg gcaggctctc atcgagcatg gtggcgagga catccacgaa ggggacatga
     2041 gcgacgaccg cgccccacag ctcgggcgcc tcgttcatca ccgcgcccat cagctcgccg
     2101 cccgccgaac cgccggagat cgagatcttg ccgggcgctg tgtagcccat ggctgcgagg
     2161 gcgcgggcga cgtcgacgaa atcgtggaag gtgttggtgc ggcgttcgag cttgccggcc
     2221 ttgtaccagg cccggccgag atcgtcgccg ccgcggatgt gggcgatggc ataggcaaag
     2281 ccgcggtcga cgagggagag gcgggtggtc gagaagccgg gggcgatggc gatgccgtag
     2341 gcgccgtagc cgtagaggtg gagcgggccg ccggccccgc cgttcatctg atcccgatcc
     2401 ttgcgcgaga cgatgctgac cgggatcttc gtcccgtcgc gcgccgggat ttcgaggcgc
     2461 tcggtgacgt agagcgaggc gtcgtagccg gaggggattt cctggacctt gcggacttcg
     2521 aggcggcgat cggcgacgtg gtagtcgtag gtggtcgccg ggctgaccat cgattcgtag
     2581 cccaggcgca ggacagccgt gtcccattcg ggattgtcgc cgaggccggc gtcgtagctg
     2641 gcttcgggaa acgcgattgg ctcgacgcgc gccgggtcgt cgtaccagcg cagttcgatc
     2701 tggtccaaac cggcgcggcg gccttcgatg acgtagaact ccttgaacag ctcgaaaccg
     2761 gtcaggtaga acgcgtccgt cccctcgatc agggtggtcc attcgccggg cgcggccagc
     2821 ggcgcggtgg ccaggcggaa gttttcgtgg gtgtcgttgg cgtggatgaa cagggtctcg
     2881 ccccgcacat cgacgtcgta ttcgataccc gccttgcgtt ccttgaccag cagcggcgcg
     2941 gccagcggat cggcggcggg gatcaagcgg acttcgctgg tttcgtgatc gctggtggag
     3001 acgaccagcc attcctcgtt ggccgagata cccgagccga cgcggaagcc ttcgtcatcc
     3061 tcgtggtaga gttcgacgtc ctcgctgacc ggacggccaa gccagtggag gcgggcattg
     3121 tcggtgcgcc actgttcgtt ggcgagggaa tagaccagcg ccttgtcgcc cgcgacccag
     3181 acgaggccgg aaagcgtgcc ctcgatcgtg tcgggcagaa tctctccggt ttcgagcacc
     3241 ttgatccggg cggtgaagcg ttcggagccg ttgtcatcga ccgaccaggc gaggagcttg
     3301 ccgtccttgc tgaccgagat cgcgccgagg cggaaatatt cgtggccttc ggcgagggcc
     3361 acctcgtcga ggatcagctc gtccgcggaa ggatcgtcct tggccgaaac gggcctgcgc
     3421 caccacttct tgtactcggc gccttcctcg tattcgatcc agtagagcca gtcgccgtcc
     3481 ttctggggga cggacttgtc ggcctccttg atgcgggcgc gcatctcggt gaacagggtt
     3541 tcgaccagcg gcttgcgctc ggccatgcgc gcctcgaacc aggcgttctc agcttcgagg
     3601 tgggcgagga tttccgggtc cttgacctcg ggatagcccg gatcgcgcag ccaggcatag
     3661 tcgtccgcga cggtgatgcc gtggtgggtg aagctgtgcg gtttcttcgc ggcgaccggg
     3721 ggcgtgttcg ggggggtgtt atcaggcatg gaccgcatct atgttgggcg gacccaagcc
     3781 gcaaccttgc gagatattta ccgatgctga tgtccgttgc cgaagcgcgc ctcgatgccc
     3841 tgcgcaagga acttgccgcc cgggggctcg acgggttcgt ggtgccgatc tcggacgagc
     3901 acatgagcga atatatcggc gcctatgccc agcggctggc ctggctgacc ggctttcgcg
     3961 gttcggcggg aacggcagtg gtgctgcgcg acgaggcggc gatcttcgtc gacgggcgct
     4021 ataccttgca ggtgcgcgat gaggtcgacg gtaagctcta tggctatgag agcgtgccgc
     4081 agacctcggt ggcgaagtgg ttgggtgagc acgcgcgcca aggcatgaag gtgggctacg
     4141 atgcctggct ccatggcaag cgctgggccg atgcggcggc caaggcgctg gccgagaggg
     4201 gcgcggaact ggtggcggta gacggcaatc cggtggatgc ggtgtgggcc gatcgccccg
     4261 cgccctcccc cgctccggcg ctggtccatc cggaggacct cgccgggcaa tcgagcgcgg
     4321 acaagcgggc ggctgtcgcg gcctggctga aggccaaggg ctgcgcggcg gcggtgattt
     4381 cggcgctgga ttcggtggcc tggctgctca acatccgggg ctccgacgtg gagcgcacac
     4441 cggtggcgct gtcgttcgcg atcgcgcgcg cggatggttc ggccgagctg ttcatcgccc
     4501 ctgaaaaggt gaccgacgac ctgcgcgcgc accttggcaa cgcggtatcc atccgcccgc
     4561 gtgacaattt cgccgcggcg ctggccgagc tggcggggca gcaggtggcg gtcgacccgg
     4621 agcgcaccgt gatgggcgtg ttcgtggcgc tcgaggccgc gggggccagt atcgtcgagg
     4681 cgcaggaccc ttgcgtgctg cccaaggcca ccaagaacgc cgccgagatc gccgggcacc
     4741 gcgctgccca ggcgcgggac ggagcggcca tggtgcattt cctgcactgg ctcagccagg
     4801 aagcgcccaa gggcacggtg gacgaactgg ctgcggccga ttggctccag cgcgcccgcg
     4861 ccgcttcggg gagcttgcgc gatctgtcgt tcgatacgat ctcgggatcg ggccccaatg
     4921 gggcgatcgt gcactaccgg gtgagcggcg agacgaaccg gccgctggag ccgggttcga
     4981 tgtacctcgt cgattcgggg gggcagtacc ccgacggcac gaccgacatc acccgcacgg
     5041 tgtggatcgg gccaggcgag ccgggggccg aggagcgcga ccggtttacc cgcgtgctca
     5101 agggcaacat tgccctgtcg cgcgcggtgt ttcccaaggg cacgctgggc agccagctcg
     5161 atacgctggc gcggcagttc ctgtggcatg ccgggatcga ctatgcccat ggcaccggcc
     5221 atggagtggg ggccttcctt tccgtccacg aaggaccgca gcggatatcg cgcgccaccg
     5281 gcggacaggg cgggaccgag caggagctgc tgcccggcat gatcctgtcc aacgagccgg
     5341 gctactacaa ggcgggggcc tggggcatcc ggatcgagaa cctgctgctg gtggaagagc
     5401 gggcgatcga gggtgccgag agcgagttcc tcggcttcga tacgctgacc catgtgccga
     5461 tcgaccgtgc cctgctcaat ccggcgatcc ttgagccagg cgaggtcgcc tggtggaacg
     5521 cctatcatgc gcgggtgctg gagatcgtcg ggccgcaggt ggacggggtg gtgctggagt
     5581 ggctgcgcga gcagtgcgcg ccgctgggga tggcgggggt gcaggggtga tgtcgtcccg
     5641 ggcttgaccc gggaccggtg tctggtgcga agctgtgagc ccgcttgccg gccaggtcct
     5701 gcctgaaggt ccgccagcgg tcccggatca agtccgggac gaccttagtc ttcagcccag
     5761 gtccagcttg tcggcgtaga tcaggcggcc gccggagagg tgccagaggg cttcgttgaa
     5821 atcggcctcg cccatggcga agcagccgtc ggagcggccg agcttgcccc atttgggcag
     5881 catgtcctcg ttggcatacc aggccgggtg gaggacgatg gcccgctcga ggacgttgga
     5941 attatcctgg tccagcccca tcagccggat cgaggtgccg tatttgccct tgtaccattc
     6001 ggccgtgaca taggcgccgc ggctggtggc gtagctgttg ggcacgttcg agaagatttt
     6061 caggaagcca tcgtgctccg gatcggagcc gcggccgtgg gcgaccagaa aggagcggac
     6121 ggagccctgt tcgagattgg cgaaatggaa ccgaggcagc gcgctgggca gggcgaaatc
     6181 ggcgacggcc gccatgtcgg cgcgccacag gcgggagcgg ttgcgctcta cctgctcggc
     6241 cgcgatcgcg aggatcttgc ggtgctgttc gctgaccgag aatgcctgcg cgaaagcgcg
     6301 ccctggcagg gcggccatgg caccggcggc aacagccccg gtcagcagcg atctccggtc
     6361 catgcagcgg gtatagcggg aatcggcgcg tttttccaga ccaatgcgat cagttgggcg
     6421 gcgtgagcac catcttgtcg cccttgagct cgaccttgcc gagctgggcg aggaccgact
     6481 ggcccaggag actgacgccg agatccttga ccacgacggc gccgacgttg tgcagttcgg
     6541 tcttgcccag ttccagccgg tcgatggtga tgagcgtgcc ccagccctgg ccggaggcgg
     6601 tctggaggat cggctggaag gcgctctggt cgaccttgat cccggcccgc tcggcatcgg
     6661 cgatggtcag tgccacggca tcggcgccgg tatcgacgag gaacttggtg ggctggccgt
     6721 tgagtgcgac atcgaggtgg aactggccgg aggcatcgcg gtcgatcacc aagccctcgg
     6781 gggtgaaccg ggcctcggca ggggcggggg catgggcggc gatcaccacg ccgccggtcg
     6841 gcccgctgag cggatggacg ggcgagagga actggccgac gagcatcagc gcgaggacga
     6901 ggcccacggc catgatgacg aagcccttca tgggcgggaa gtctaggggg gcagggttaa
     6961 tttattccgc tgcttgcggc tcggccttgc ccgcatcggt ggtttcagcc tcgagctgcg
     7021 cggccttggc ctcgacgagg ctgacgatgt gatcgagcat ggcctcggac tggacgtggt
     7081 ggtcggtcac ccccgagaga tagaccatgt gcttgccgtt gccgccgccg gtgaggccga
     7141 tgtcggtttc gcgcgcttcg cccgggccgt tgacgacgca gccgaggacc gagagcgaga
     7201 gcggggtctt gatgtgggac aggcgctttt cgagggcctc gaccgtgcgg atcacgtcga
     7261 acccctggcg ggcgcagctc gggcaggaga cgatacgcac gccccgggtg cgcaggtcga
     7321 gcgccttgag gatctcgaag ccgacgcgca cttcctcttc cggctcggcc gagagcgaga
     7381 cgcggatggt atcgccgatg ccggcccaga gcagggagcc gatgccgatg gagctcttga
     7441 ccgtgccgcc gatcaggccg ccggcctcgg tgatgccaag atgcagcggg caatcgacgg
     7501 cttcggacag gccctggtag gcggcgacgg cgaggaacac gtcggaggcc ttcaccgcca
     7561 ccttgtattc gtggaaatcg tgatcctgca gcagcttgat gtgatcgagc gcgctttcca
     7621 cgagtgcttc ggggcagggc tcgccatact tttccaacag gtccttctcg agactgccgg
     7681 cgttgacgcc gatgcggatc gagcagccgt tggccttggc ggcgcggacg acctcggcga
     7741 cgcgatcgga cgagccgatg ttgccgggat tgatgcgcag gcaggccgcg ccggcatcgg
     7801 cggcttcgag ggcgcgcttg tagtggaagt ggatgtcggc gacgagcggc acggccgcgg
     7861 cgcgggcgat ctcgcggaag gcggcggtgc tggcctcgtc cgggcaggaa acgcggatga
     7921 tatccgcccc ggcatcctcg cagcggcgga tctggctgat cgtcgccccg gcgtcggagg
     7981 tgagggtgtt ggtcatggtc tggaccgtga tcggcgcatc gccgcccacc ggcaccttgc
     8041 cgaccatgat ctgccggctc ttgcggcgaa caatcgtgcg ccaaggtctg atcgaggaca
     8101 tggcggggga tataggggcg cggggggatt cgggcaacgt gccccgtggg cccgcccgcg
     8161 cccccttcga ttatccttcc gcttcccgct cgaacgctgc ttctccgcca atcttcgccg
     8221 agacgcggcc gagcacggtc agcacgacca cggccagcac ggtggcgagg atggcgtagg
     8281 agtagaggcc ggcgaggtcc tcgcccatgc cgagcttggc gagggtggcc ttgatcgcct
     8341 cattccaggc caaggcggcg acgaggccga gcgaggcgga ggcgagggcg atcatcgtct
     8401 ggatcatggc tttcgggtcg gtcatggcag gagcccctgg ttcgtgaacg gtgcccgatg
     8461 gtggcaaagt cgggggcgag gggcaagcaa gcgcgccgtg gccgttcaca gcatgcgcgc
     8521 gaagaggaac tcctcgtcga gctgttcgcc gacgcggaag ctgacttctg ccacctgatc
     8581 aaacccatag cgccggtaga accgctgggc gcccgggttg tgccgccaga cggagagctg
     8641 gacctcgtcg gcgccgagcg cggcgaactc ggccatggcc cagtccatca gcgcggcgcc
     8701 gatcccggcc ccttgcgcat tgggcgcggt gtagagctgt ttcaactcca tggtccgcgc
     8761 gccgcgggaa tgatctggga aagagcagtg gaggccgagc ttggcccagc ctagcagcag
     8821 gccccgcgct cggccacctg atagcgggtc agcgggtcgg cgatggccat ggcgagagcg
     8881 gcttcggagt gggtttcggc aaggaacggc agcaggtctt ccgggcggta cagatcgccg
     8941 aacttgtgca cgaaggcggc gacgccgagc agcga
//
LOCUS       DATNBV010000267         8139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2717656,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000267 DATNBV010000000
VERSION     DATNBV010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2717656"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VN627_06435"
     CDS             <1..897
                     /locus_tag="VN627_06435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXD02929.1"
                     /translation="RFEGLPAVPVLRTPESWEDMLFAAQLAAWLNPADFDAVVTCSFP
                     FTHWALRRPGRGGRPLQVFVTQNGDWPARRESSEFRTFRCDGLVCTNPDFHERHRAAW
                     PSALIPNGVDLARFGTGRHPPSAFGLADDRPVVLMVSALIESKRVADGVAAVAGLPDA
                     QLIVAGDGPLRDEIDRLGADRLGPRYRRVTLPAVEMQTLYRAADAFLHLSTDESFGNV
                     FVEARASGLPAVAHDLPRTRWIVGEDQFLCDTTDPRALTAALGAALARGPGAPAADIG
                     RFAWPTVAAQYRTFIASLKGAA"
     gene            894..2099
                     /locus_tag="VN627_06440"
     CDS             894..2099
                     /locus_tag="VN627_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXD02930.1"
                     /translation="MSGLLDAASWVVAGAAAVPCLVLSGEIALGLAGCPDRTTPAPAA
                     RVAVIVPAHDEAAVIEGSLRALMAAVPPGTRILVVADNCSDATAAIARGTGAEVIERT
                     DRERRGKGYALAFARDHLAAGLPGTQPEVVLVLDADCRLAPGSAERLAGSCLATGRPA
                     QARNLLTAPGGEASPLVQISCFAFALKNLVRARGLQRMAGAITLMGTGMAFPWPMFAR
                     LPLASGHLAEDMELGVELIRQGQGAVLVPAASVTSPHAARSDTLEQRKRWEHGFLSVA
                     ARQALPLLGEGLARRRSSLIALGLDLLVPPLALMGVLVLGAGVVLALGWLAGASAAPL
                     IALGASAGLAGVLVLAAWGVMGGEMLRPGALLRVPLYIIWKLPLYLRFLARRETRWVR
                     TRRPGSERG"
     gene            complement(2121..2981)
                     /locus_tag="VN627_06445"
     CDS             complement(2121..2981)
                     /locus_tag="VN627_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HXD02931.1"
                     /translation="MLSTPYAFEPAAPLAEAGDALNFINDNDYSSPRSKISFLGVEFD
                     DVDMGEVLRHCDVLTRSHDFHYITTPNVDHIVTMHSEQSHARTFRQAEKGATIRLCDS
                     RILALLARISGIRLAAVPGSDLTCELLRKVIRAGHRVAIVGGDEALLQCLRERRPGVT
                     FLQHTPPMGVLHDKAAQDAICRFVEAADADFVFIAIGAPQSEVVARAIARSGKARGLG
                     LCVGASLAFFTGQLRRAPRPLQVMHLEWLFRLVSEPRRLWHRYVVKGPRIFRIWWDYQ
                     TAGRHGAPAF"
     gene            3303..3809
                     /locus_tag="VN627_06450"
     CDS             3303..3809
                     /locus_tag="VN627_06450"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXD02932.1"
                     /translation="MEVGSDGARWVDAPGAAAPAPAAGLSEIPATAVGAAPTGVPDRW
                     RNHVYFTAYKYGLSPDLLEAVVWQESRWRPDAVSPAGARGLTQLMPGTARQLGVDPAD
                     PLAALDGGARYLRMQLDLFGGDVERALAAYNAGPGRVQRAGRVPNIRETQIYVASIMA
                     RLTHKLGV"
     gene            3813..4118
                     /locus_tag="VN627_06455"
     CDS             3813..4118
                     /locus_tag="VN627_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HXD02933.1"
                     /translation="MSKSFKLSATALLLAMPGTAFAQAGGPQGSGPIIAALSWLQGTL
                     LGNVATAVAVMAVAAVGFMMLTGRMNWRFGATVIIGCFVLFGAGAIVSGIQSVAVTG"
     gene            4125..4421
                     /locus_tag="VN627_06460"
     CDS             4125..4421
                     /locus_tag="VN627_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HXD02934.1"
                     /translation="MALPATRIAQFPVHRALTRPQMFAGVTYNFFVINMALTTEVFLI
                     THRFMALPIALLIHAAGYLASLREPRVFDLWLTKASKCPRTPNRQLWGCNSYAP"
     gene            4418..6910
                     /locus_tag="VN627_06465"
     CDS             4418..6910
                     /locus_tag="VN627_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HXD02935.1"
                     /translation="MIRGKSSSRKGGNGGGHSWAGREALAGDRLPYHSLLDENVVLLR
                     DGSVMISLLVPGLSFETAGEDELNSHTALRELLMRSALDSRFILYHHVIRRRVEVELE
                     SPFADPLAAHIDMRWRQRLRGGAMFVNDQFVTLVRRPARGKAGWPELLSRRLSRGLRR
                     SGNRAPTANPEDVRALRAAASAMIVALGEYGARLLGDYRGSAGPCNEVLELLSALYNG
                     ELRPVRRPSESADLGQVLPYRRLAFGLDALESQGLGGSTFGTMISLKDYPDSTTPGLV
                     DPLLRLPCELVLTESYAPSDRQIARERIDLALRRLRSADETAMAERREMQSARDALGV
                     GQVGFGDHHLSVLVRSNQLDLLDEAGAACAAALAEAGAIAVREDVNLEPAFWGQFPGN
                     ESYVVRRAMISSANMACFGSLHGFAIGHASGNHWGEAVSLLQTTSATPFFFNFHGTGT
                     GGGDLGNFTVIGPSGSGKTVVMNFLAAQAQRFAPRTILFDKDRGAEVFVRGIGGTYSR
                     IATGHPTGFNPLALADSPANRGFLCDWLGVLLAARGPEETALIAQAVDAAYANHPRLR
                     RLTHFRELLAGRGRPTPGDLADRLGPWIEGGEHGWLFDGPVLDGADGPADRLDLSAAT
                     LGFDMTALLENPRLRTPTMMYLFHRIEERLDGTPTLILIDEGWKALDDEVFAARIRDW
                     LKTLRKRNALVGFATQSARDALDSRIATALVEQTATMIFMPNSRARPEDYCEGFGLTE
                     REFQIIRTLPAHSRCFLVRQPDASVVVRLDLAGMPDVLTVLSGRESIVRRLDTLRAEL
                     GDEPAAWFPSLTGMPWPGSENLELGWFLEAAE"
     gene            6907..8061
                     /locus_tag="VN627_06470"
     CDS             6907..8061
                     /locus_tag="VN627_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HXD02936.1"
                     /translation="MSACDALTGAAADGTLQAIRAVDCLANETAAMGFARLFGTQGSL
                     LSALTILLTLFIAIFALALLTGRTRIGVAVLTPQVFLLGAVLTFATSWVAYQSVIWNL
                     AVAAPDELAGTLMGTAGSAVELFASGLDQILAAIAAAADAVGTPAAGAAQATQGSFTP
                     TNLMWLAALLLMLGTVGVIVVSRIALGVLLAVGPVFIVMALFSGTRGLFAGWLRAVVL
                     TGITPLFAVLGGTMSFQLIVPVLQKLNGDLGIDGRAAISLFLVSAVHCALMVLSMKVC
                     STLVGAWQVFGGRADRASDPAPAPAWQAPAAPLLLPSPAALRTIHAAALPPESAALPG
                     GAPALLTGSQLRLIDGPGHAHTPLAAGAHARARGIGSRFRPLPKPQELLR"
BASE COUNT         1101 a         2947 c         2724 g         1367 t
ORIGIN      
        1 cggttcgaag gcttgcccgc cgtgccggtg ctgcgcacgc cggagagctg ggaggacatg
       61 ctcttcgccg cgcagctggc ggcgtggctg aacccggccg atttcgatgc ggtggtcacc
      121 tgctcgtttc ccttcaccca ctgggcgctg cgccgccccg gccggggcgg gcgccccttg
      181 caggtcttcg tcacacagaa cggcgattgg ccggcccggc gggagtcgtc ggaattccgc
      241 accttccgct gcgacgggct ggtctgcacc aatcccgatt tccatgaacg ccatcgcgcc
      301 gcttggccga gcgcgctgat ccccaacggg gtggacctgg cccgcttcgg cacgggccgt
      361 cacccgccct cggccttcgg cctggccgac gaccggccgg tcgtgctgat ggtcagcgcc
      421 ctgatcgaga gcaagcgcgt cgccgacggg gtggctgccg tcgcgggcct gcccgatgca
      481 cagctgatcg tggccggaga cgggccgctg cgcgacgaga tcgaccggct gggggccgac
      541 cggctcggcc cgcgctatcg ccgggtaacc ctgccggcgg tggaaatgca gacgctctac
      601 cgcgccgcgg acgccttcct gcatctctcg accgacgaat cctttggcaa tgtcttcgtg
      661 gaggcccggg ccagtggcct gcccgcagta gcccatgatc tgccccgcac gcgctggatc
      721 gtcggcgagg accagttcct ctgcgacacc acagatcccc gggcgctgac cgccgcgctc
      781 ggcgcggctc tcgcgcgggg gccgggagca cctgcggccg acatcggccg cttcgcctgg
      841 ccgacagtcg ccgcgcagta ccgcacgttc atcgcaagcc tcaagggggc ggcatgagcg
      901 gcttgcttga tgcggctagc tgggtcgtgg cgggcgccgc cgccgtgccg tgcctcgtct
      961 tgtcagggga gatcgcgctc ggcctcgccg gctgcccgga ccggacgacg cccgcacccg
     1021 ctgcccgggt tgcggtgatc gtccccgcgc atgacgaggc ggcggtgatc gaaggttcgc
     1081 tgcgcgcctt gatggccgca gttccgccgg gcacccgcat cctcgttgtc gccgacaact
     1141 gcagcgatgc cactgcagcc atcgcccgcg gcaccggagc agaggtgatc gagcggaccg
     1201 atcgcgagcg ccgcggcaag ggctatgccc tggcctttgc ccgcgatcac cttgccgccg
     1261 gtttgccggg gacccagccc gaggtggtgc tggtgctgga tgccgattgc cggctggccc
     1321 ccggctcggc cgagcggctg gccggctcct gccttgccac cgggcgcccg gcccaggccc
     1381 gcaacctgct caccgcgccg ggtggagagg cgagcccgct ggtgcagatt tcctgctttg
     1441 cctttgcgct caagaacctg gtccgcgcgc gcgggctgca gcgcatggcg ggcgcgatca
     1501 ccttgatggg caccggcatg gccttcccct ggccgatgtt cgcccggctg ccgctcgcca
     1561 gcgggcatct ggcggaagac atggaactag gcgtcgaact gatccggcag gggcagggcg
     1621 ccgtgctggt gcccgccgcc tcggtgacct cgccccacgc ggcgcgctcc gatacgctgg
     1681 agcagcgcaa gcggtgggag catggcttcc tgtccgtcgc cgcgcggcag gcgctgccgc
     1741 tgctgggcga aggcctggcc aggcggcgct cgtcgctcat tgcgctgggg ctggacctgc
     1801 tggtgccgcc gctcgcactt atgggcgtgc tcgtgctcgg cgccggcgtg gtgctcgccc
     1861 tgggctggct ggctggtgca tcggcggcgc cgctgatcgc gctgggtgcc agtgccggat
     1921 tggctggagt tctggtcctc gcggcatggg gcgtgatggg cggggagatg cttcgacccg
     1981 gcgccctgct cagggtgccg ctatatataa tctggaagct gccgctttat ctgcgctttc
     2041 tcgcgcggcg cgagacccgg tgggtcagga cccggcgccc cggatcggag cgtggttgac
     2101 ccgccgggtg gtactccggc tcaaaaggcc ggagcgccgt gccggcccgc cgtctggtag
     2161 tcccaccaga tcctgaaaat ccggggaccc ttgaccacgt agcggtgcca cagccgccgg
     2221 ggctccgaca ccaggcgaaa cagccattcg aggtgcatca cctgcagggg acgcggcgcg
     2281 cggcgcagct ggccggtgaa gaaggccagc gaagcgccaa cgcaaagccc gaggccgcgg
     2341 gccttgcccg accgggcaat ggcgcgggcg accacctcgc tctgcggtgc gccgatggcg
     2401 atgaacacga aatcggcatc agctgcttcg acgaatcggc agatcgcatc ctgcgccgcc
     2461 ttgtcgtgga gcacgcccat cggtggtgtg tgctggagga aggttacgcc tggccgccgc
     2521 tcgcgcaggc attgcagcag ggcttcgtcg ccaccgacga tggctacgcg gtgcccggca
     2581 cggatgacct tgcggagcag ttcgcaggtc aggtcgctgc ccggcaccgc ggcaagccga
     2641 ataccggaaa tccgtgccag cagggcgagg atgcggctgt cgcacaggcg gatggtcgcg
     2701 cccttttcag cctggcggaa ggtcctcgcg tgcgactgtt cggagtgcat cgtgacgata
     2761 tggtcgacgt tcggcgtcgt aatatagtga aagtcatgac tacgggtcag aacatcgcag
     2821 tgtcttagaa cctcccccat atctacatcg tcgaactcga cgccgaggaa tgatatcttc
     2881 gatcgtggtg agctgtagtc attatcgttg atgaaattca gcgcatctcc cgcctcggca
     2941 agcggggcgg caggttcaaa cgcgtagggg gtagatagca tcgacaggtg ctcctcgacg
     3001 ctaacaactt gttaaccatt cgcccgttcc cgcgctgcgg caaactgtcc cgctttgggg
     3061 cgggtcactt ttcgaactgc gcacagccct tcggcagggt gaccgattaa tgcgccgggg
     3121 cgacatcctg cccgcccgcc ttaacttggc tttaaccact caatctgtag gaaagagttg
     3181 ggcaaactca gattcttagc tgggtttgag gacgggagta tgcagtgttc catagagcca
     3241 ttccggctac ccttgcggtt atcctgagcg tgctctggac gggggccgtc caggcccagg
     3301 tgatggaggt cggcagcgat ggtgcgcggt gggtcgatgc ccccggcgcg gctgcaccgg
     3361 cccccgccgc aggcctgtcg gagatcccgg cgaccgccgt gggcgccgcg ccgaccggcg
     3421 tgcctgaccg ctggcgcaac catgtatatt tcacggccta caagtatggc ctaagtccag
     3481 atttgctcga agcagtagtc tggcaagaaa gccgctggcg tcccgatgcc gtctccccgg
     3541 ccggcgcgcg cgggctgacc cagctgatgc cggggaccgc ccgccagctc ggcgtcgatc
     3601 cggccgatcc gctcgccgcg ctcgatggcg gggcgcggta cctgcgcatg cagctcgacc
     3661 tgttcggtgg cgacgtcgaa cgggcgctcg ccgcctacaa tgccggcccc gggcgcgttc
     3721 agcgcgctgg ccgggtccct aacattcgcg aaacccagat ttacgtggcc tcgatcatgg
     3781 cccgtctcac tcacaaactc ggggtctgac ccatgtccaa gtcattcaag ctctctgcca
     3841 ccgctctcct cctcgccatg cccggcacgg ccttcgccca ggcgggcggg ccgcagggtt
     3901 ccggcccgat catcgccgcg ctcagctggc tccagggcac cctgctcggc aatgtcgcca
     3961 cggccgtggc ggtcatggcg gttgctgccg tcggcttcat gatgctgacc ggccgcatga
     4021 actggcgctt cggggccacc gtgatcatcg gctgcttcgt cctgttcggg gcgggggcga
     4081 tcgtctcggg catccagtcc gtcgcggtaa cgggctgacc gggcgtggcc ctcccagcta
     4141 ccaggatcgc ccagttcccc gtccaccggg cgctgacccg tccccagatg ttcgccgggg
     4201 tcacctacaa cttcttcgtg atcaacatgg cgctcaccac cgaggtgttc ctgatcaccc
     4261 accggttcat ggccctgccc atcgccctgc tgatccatgc ggccggctac ctcgcctccc
     4321 tgcgcgagcc gcgcgtgttc gacctctggc tgacgaaagc gagcaaatgc ccacgcactc
     4381 cgaaccggca gctctggggc tgcaacagct acgcgccatg atcaggggca agtcatccag
     4441 ccgcaagggc ggtaatggcg gaggccattc atgggccggg cgcgaggccc tggccgggga
     4501 ccgcctcccc tatcattcgc tgctcgacga gaacgtcgtc ctgctgcgtg atggctcggt
     4561 gatgatctcg ctgctggtgc ccggcctcag cttcgagacg gcgggcgagg acgagctcaa
     4621 cagccacacc gccttgcgcg aactgctgat gcgcagcgcg ctcgacagcc gcttcatact
     4681 ctaccaccac gtgatccgcc gccgggtcga ggtcgagctg gaatcgccct tcgccgatcc
     4741 gctggctgcc cacatcgaca tgcgctggcg ccagcgcctg cgcggcgggg cgatgttcgt
     4801 gaacgaccag ttcgtcaccc tcgtccgccg ccccgcccgc ggcaaggcgg gttggccaga
     4861 actgctgtca cggcgcctgt cgcgcgggct gcgccgcagc ggcaaccgcg cgcccacggc
     4921 caatcccgag gacgtgcggg ccctgcgcgc cgcggcctcg gcaatgatcg tcgcgcttgg
     4981 cgaatatggc gcacgcctgc tcggcgacta tcgcggttcg gccgggccct gcaacgaagt
     5041 gctcgagctg ctttcggcgc tctacaatgg cgagctgcgc cccgttcgcc ggccttcgga
     5101 gagcgccgat ctcggccagg tcctgcctta tcgccgtctc gccttcgggc tcgatgcgct
     5161 ggagagccaa ggccttggcg gctcgacctt cgggacgatg atctcgctca aggactaccc
     5221 ggattcgacc acgcccgggc tcgtcgatcc cctgctgcgc ctgccctgcg agctggtcct
     5281 caccgaaagc tatgccccgt ccgaccggca gattgcccgc gagcggatcg acctcgccct
     5341 gcgccgcctg cgctcggccg acgagaccgc catggccgaa cggcgcgaga tgcaatcggc
     5401 gcgcgatgcg ctgggggtcg gccaggtcgg cttcggcgat catcaccttt ccgtgctggt
     5461 gcgcagcaac cagcttgacc tgctcgacga ggccggggcc gcctgcgccg ccgcccttgc
     5521 cgaggccgga gccatcgccg ttcgcgagga cgtcaacctc gagcccgcct tctggggcca
     5581 gttccccggc aacgagagct acgtcgtgcg ccgggcgatg atctccagcg ccaacatggc
     5641 ctgcttcggc tcgcttcacg gcttcgccat cggccacgcc agcggcaacc actggggcga
     5701 ggcggtctcg ctgctgcaga ccaccagcgc gaccccgttc ttcttcaact tccacggcac
     5761 cggcaccggc ggcggcgatc tcggcaactt caccgtcatc gggccttccg gctcgggcaa
     5821 gaccgtggtg atgaacttcc tcgccgccca ggcccagcgc ttcgccccgc gcacgatcct
     5881 gttcgacaag gatcgcggcg ccgaggtttt cgtgcgcggc atcggcggca cctacagccg
     5941 catcgccacc ggccatccca ctggcttcaa cccgctggcc ctggccgaca gccccgccaa
     6001 ccgcggcttc ctgtgcgact ggctgggcgt gctgctggcg gcccgggggc cggaggaaac
     6061 agccctgatc gcccaggcgg tcgacgccgc ctatgccaac cacccgcgcc tgcgccgcct
     6121 gacccatttc cgcgagctgc tggccgggcg cggccgcccg accccgggtg atctggccga
     6181 ccggcttggc ccctggatcg agggcggcga acacggctgg ctgttcgacg gaccggtcct
     6241 cgacggagcg gacggcccgg ccgaccggct cgacctctcc gccgccacgc tcggcttcga
     6301 catgaccgcg ctgctcgaaa acccgcgcct gcgcacgccg acgatgatgt acctgttcca
     6361 ccggatcgag gagcggctcg acgggacccc gacgctgatc ctcatcgacg aaggctggaa
     6421 ggcgcttgac gacgaggtct tcgccgcccg catccgcgac tggctcaaga cgctgcgcaa
     6481 gcgcaatgcc cttgtgggat tcgcgaccca gtcggcccgc gatgcgcttg acagccggat
     6541 cgccacggcc ctggtcgaac agaccgcaac gatgatcttc atgcccaatt cgcgggcccg
     6601 gcccgaggac tactgcgagg gcttcggcct gaccgagcgg gaattccaga tcatccgcac
     6661 cctgccggcc cacagccgct gcttcctcgt gcgccagccc gatgcctcgg tggtggtgcg
     6721 gctggatctc gcgggaatgc ccgatgtgct caccgtcctt tccgggcggg aatcgatcgt
     6781 gcgccggctc gacacgctgc gcgccgagct gggtgacgag cccgccgcct ggttcccttc
     6841 gctcaccggc atgccgtggc ccggctctga aaacctcgaa ctcggctggt tcctggaggc
     6901 ggcggagtga gcgcctgcga tgccctcacc ggcgcggcgg cggacggcac cctgcaggcg
     6961 atccgcgcgg tcgactgcct ggccaacgaa accgccgcca tgggctttgc ccgcctgttc
     7021 ggcacccagg gctcgctgct ctcggccctg accatactgc tgaccctgtt cattgcaatc
     7081 tttgcgctgg ccctgctgac cgggcgcacg cggatcggcg tggccgtgct gaccccgcag
     7141 gttttcctgc tcggcgccgt gctcaccttc gcgacctcgt gggtcgccta ccagagcgtg
     7201 atctggaatc tcgccgtggc cgcgccggac gaactggccg gcaccctgat gggcacggcg
     7261 ggttcggcgg tggagctgtt cgccagcggg cttgaccaga tccttgccgc catcgccgca
     7321 gccgccgatg ccgtgggcac gcccgctgcc ggggccgcgc aggcgaccca gggcagcttc
     7381 acgcccacca acctgatgtg gctcgccgcc ctgctgctga tgctgggaac ggtcggcgtg
     7441 atcgtcgtct cgcgtattgc gctgggcgta ctgctggcgg ttggcccggt gttcatcgtc
     7501 atggcgctgt tttccggcac gcgcgggctc ttcgccggct ggctgcgcgc cgtggtgctg
     7561 accgggatca ccccactgtt cgccgtgctg ggcgggacga tgagcttcca gctgatcgtc
     7621 ccggtgctgc agaagctcaa cggcgatctc gggatcgacg ggcgtgcggc gatatcgcta
     7681 ttcctcgtct cggccgtcca ctgcgcgctg atggtcctgt cgatgaaggt ttgctcgacc
     7741 ctggtcggcg cctggcaggt cttcggcggc cgggcggacc gcgccagcga cccggctccc
     7801 gcccccgcct ggcaggcacc tgccgccccg ctgctgctgc cttcgcccgc cgcgctgcgg
     7861 accattcacg ccgccgccct tccgcccgag tccgccgcac tgcccggcgg tgccccggcg
     7921 ctgctcaccg gatcgcagct gcgcctgatc gatggccccg gtcacgccca caccccgctc
     7981 gccgccggcg cccacgcccg ggcgcgcggc atcggcagcc gctttcgccc gctgcccaag
     8041 ccccaggagc tcctgagatg attgcccgct tccttgttgc cgcggcaggc gccgccgcgc
     8101 tcgccgccgg cgccgccagt ccggccgccg ccgccgacg
//
LOCUS       DATNBV010000268         2234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4298959,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000268 DATNBV010000000
VERSION     DATNBV010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4298959"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1406
                     /locus_tag="VN627_06475"
     CDS             342..1406
                     /locus_tag="VN627_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011046377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXD02937.1"
                     /translation="MPRQSTDTVAVLGIDIGKNTFHLVGLNERGAIILRRKLSRGQLE
                     AKLANLPPCLVGMEACVGAHHLSRRLKALGHDARLMPARYVRAYLKGNKNDFRDAEAI
                     AEAVQRPTMRFVATKTAEQLDLQALHRVRSRLVGERTAIANEIRAFLLDRGIAVAKGL
                     NRLRAALPGILGTRTEVLSPRLLHIIEDLVADWRHLDQRIDNVSLQIEALAKQDAGCE
                     RLMTVPGVGPIISSATVAAIGSGDVFAKGRDFSAWLGLVPKQISTGDRTILGKISKRG
                     NKYLRTLFVQAARVVLLWPRSWERYGLKPWIEAAARRLHHNVLAIALANKLARIAWSV
                     LHHGRNFEVRKMSEPVSRSA"
     gene            2039..>2234
                     /locus_tag="VN627_06480"
     CDS             2039..>2234
                     /locus_tag="VN627_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HXD02938.1"
                     /translation="MGLLDDLTSPPKERYAVSNGNVVRLIQPGAFEDRLTEILRGGAR
                     SLLAQAIEAEVAEFLASHADL"
BASE COUNT          410 a          764 c          696 g          364 t
ORIGIN      
        1 atagggcttg tcccatgcga gccgctcaag caacgggatc gtgcgtaatg ccgtgcgcgc
       61 cgccaccgcc agcgacccca acccattcgg cccccccaca tcgatcaggc ggtcccgcaa
      121 cgcatagact tctttggtcg ccatcacgtc actccccacc cactgcatct cacgacggcg
      181 ccgccgagta tcgccgatgt tatggcttgt tcgcggctgc cgttgggcca gcagacctgc
      241 cagaatccta aacgggcgat gtccgctaat gggatggacc ggcggtgctc cccgcagtac
      301 cccgcgtcga acgggggtaa gcggcagtaa ggagccccgc catgcctcgc cagtccacag
      361 acaccgtcgc cgtcctcggc atcgacatcg gcaagaacac cttccacctc gtcggcctca
      421 atgagcgcgg cgccatcatc ttgcgccgga agctctcgcg cgggcaactc gaagccaagc
      481 tcgccaacct gccgccctgc ctggtcggga tggaggcctg cgtcggcgcc caccacctga
      541 gccggcggct caaggccctc ggccacgatg ccagactgat gccggcccgc tatgtcaggg
      601 cctacctcaa gggcaacaag aacgacttcc gcgatgcgga ggcgatcgcc gaagccgtgc
      661 agcgtccgac catgcgcttc gttgccacca agaccgccga gcagctcgat ctgcaggcgc
      721 tgcaccgggt gcgctcacgt ctggtcggcg agcgcacggc catcgccaac gagatccgcg
      781 ccttcctcct cgaccgcggc atcgccgtcg ccaagggtct caaccggttg cgggctgcgc
      841 tgccgggcat cctgggcacg cgcacagaag tcctgtcccc gcgcctcctg cacatcatcg
      901 aggacctggt cgccgactgg cgccacctcg atcagcgcat cgacaatgtc tcgctgcaga
      961 tcgaggctct cgccaagcag gacgccggct gcgagcggct gatgacggta ccaggcgtcg
     1021 gtccgatcat ttccagcgcc acggtggccg cgatcggcag cggcgacgtg ttcgccaaag
     1081 gacgagactt cagcgcctgg ctcggtctgg tccccaagca gatctcgacc ggtgatcgca
     1141 ccatcctcgg taagatctcc aagcgcggca acaagtacct gcgaacgctg ttcgtgcaag
     1201 ccgcccgcgt cgtgttgctg tggccccgca gctgggagcg ctacggcctc aagccctgga
     1261 tcgaggcggc cgcccggcgg ctgcaccaca acgtgctggc gatcgcgctc gccaacaagc
     1321 tcgcgcgcat cgcctggagc gtgctccacc acggccgcaa cttcgaggtc aggaagatgt
     1381 cagaacccgt gtcccggtcc gcgtgaggcg ggccccgaca tgacgagcgt gccgtcgtgc
     1441 tcgacgctgt ccaggccaag cccttcgggt ggccctgaac cgggccagcc tggacagcat
     1501 ctgcgcgcgc cggcctcggc attctgcggt cggggcggga gaaagcctgc ggcgcggtcg
     1561 aacaagagaa atggacagaa gaaaaggtga tgatgccagc ttgaggcccg attcccaccg
     1621 aggtctgcga gagagaaagg aaagcgatgg agcaaccggt cttcccggcg catgcgagca
     1681 ctggtgaccc aaacagcccg ctcgaggctt gtccgctaat cagatcgaat gcgcgctcat
     1741 atccatgatg gccgggagcc tatgagctcc acccaaaggc cggatacatt ggtgcgagac
     1801 cagaatgctc caggttcacc gatttctctt gcgacgcacc gccggtccat acattgggtc
     1861 acctttcccc ggtcccgcat cggccgcggc acggcgaatg ttactctgta accggacggg
     1921 ctctcgcggc tatcggcacg ggcaggaagt gccagaaggc gacattgacg aatttggttg
     1981 agggcgctga gctatcgccg gaagttggtg acggatcgaa tcgggaaggc ggcatatcgt
     2041 ggggttgctt gacgacctca cttctccacc gaaggagcga tatgccgtgt ccaacggtaa
     2101 cgttgtccgt ctgattcagc caggagcatt cgaggaccgg ctcaccgaga tcttgcgcgg
     2161 cggggcgcgt agcttgctgg cgcaggcgat cgaggccgag gtcgccgagt tcctcgccag
     2221 ccatgccgat ctca
//
LOCUS       DATNBV010000269         2666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_806210,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000269 DATNBV010000000
VERSION     DATNBV010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806210"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1631
                     /gene="rpoN"
                     /locus_tag="VN627_06485"
     CDS             144..1631
                     /gene="rpoN"
                     /locus_tag="VN627_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HXD02939.1"
                     /translation="MVLGPRLNLRQSQSLVMTPQLQQAIKLLALSNLEVETFIGEALE
                     GNPLLDIGAPAATPEEAAPDDDHRRTTLENSPVDQLIGEGRAADDRPLDIDAAALDTD
                     RDTGDLAFLPDLPSGASGGEAPDIDDRGALAETLAEHLHAQVGAQARDPQVAFIAHAL
                     VDQLDEAGYLALPLGEAAADLGVSLAEAEAGLALLQSLDPTGVGARSLAECLALQARE
                     ADRYDPCMARLLDNLDLLARGELARLKRMCGVDDEDFAGMLAELRGYDPKPGLRFGAA
                     SAEPVVPDVLVRPRADGGWSIAINQATLPRLVVNRSYYVELRGTCGDKASRGWLSEKL
                     ADANWLIKALDQRQKTILKVAAEIVRQQDGFFRHGVAHLKPLTLRAVAETIGMHESTV
                     SRVTSNKYLNCARGTFELKFFFTSGVASADGEGAVSAEAVKAAIRQLIDAEDPGAILS
                     DDALVDLLKGKGFDLARRTVAKYREAIGLGSSVQRRRQKALAGVR"
     gene            1923..2447
                     /locus_tag="VN627_06490"
     CDS             1923..2447
                     /locus_tag="VN627_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HXD02940.1"
                     /translation="MSNDHTLASAGTGAAIGAAGGAGVAALAGGDVLAGAAIGAAVGG
                     IAGAVWADRDGDGRVDGYVREGQYYPGVPQGYDPNYQSAAAAAPACRSVGGTAVTGAA
                     VGGVGGAAVGALAGNVLAGAVLGAAVGGLAGAVWADHNNDGCVDGYVREGRYYEGAPV
                     IQPTDNYRRTGERG"
     gene            2452..>2666
                     /locus_tag="VN627_06495"
     CDS             2452..>2666
                     /locus_tag="VN627_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02941.1"
                     /translation="MSSATSPSARHPRYRRLLAPVALALALGLVPGAPGPTLAQAQAV
                     APSMRAVLADRAPGDLRAFYAARGWQPL"
BASE COUNT          386 a          982 c          902 g          396 t
ORIGIN      
        1 acgaacatcc gggggatgtt cgtgtggtag gaggccggac acccatcgcg tgtaccctgt
       61 gggtggccgg gccgggggag cgggccggtg ccgcaagctt ccgacggatg tcggaagcgc
      121 acccgacgaa gaccacctga accatggtcc tggggccgcg cctcaatctc cggcagagcc
      181 agtcgctggt gatgacgccg cagctccagc aggcgatcaa gctgctggcg ctgtcgaacc
      241 tcgaggtcga aaccttcatc ggcgaggcgc tcgagggcaa tccgctgctc gatatcggcg
      301 cccccgcagc cactccggaa gaagccgctc cggatgacga tcaccgccgc accacgctag
      361 aaaattcgcc ggtcgaccag ctgatcggcg aaggccgcgc agcggatgac cgtccgctcg
      421 atatcgacgc cgccgcgctc gataccgatc gtgacaccgg cgatctcgcc ttcctgcccg
      481 acctgccgtc cggcgcttcg ggcggcgagg cgcccgatat cgatgatcgc ggggcccttg
      541 ccgaaaccct cgccgaacat ctccacgccc aggtcggcgc ccaggcgcgc gatccgcagg
      601 tcgccttcat cgcccatgcc ctggtggatc agcttgacga ggccggctac ctcgccctgc
      661 cgctcggcga agccgccgcc gatctcggcg tttcgctggc cgaggccgag gccggccttg
      721 cgctcctcca gtcgctcgat cccaccggcg tcggcgcgcg cagcctcgcc gaatgcctcg
      781 ccctccaggc acgcgaggcg gatcgttacg atccctgcat ggcccgcctg ctcgacaatc
      841 tcgacctgct cgcccgcggc gaactcgccc gcctcaagcg catgtgcggc gtggatgacg
      901 aggatttcgc cggcatgctc gccgaactgc gcggctacga tcccaagccc ggcctgcgct
      961 tcggcgcggc cagtgccgag ccggtcgtgc ccgacgtgct ggtgcgtccg cgggcggatg
     1021 ggggctggtc gatcgcgatc aaccaggcca ccttgccccg cctcgtcgtc aaccgctcct
     1081 actatgtcga gctgcgcggc acctgcgggg acaaggcttc gcgcgggtgg ctgtcggaaa
     1141 agctggccga tgccaactgg ctgatcaagg cgctggacca gcgccagaaa accatcctca
     1201 aggtcgccgc cgagatcgtg cgccagcagg acgggttctt ccgccacggc gtcgcccatc
     1261 tgaagccgct gaccctgcgc gccgtcgccg aaaccatcgg catgcacgag agcaccgtca
     1321 gccgggtcac gtccaacaag tacctcaact gcgcgcgcgg caccttcgag ctgaagttct
     1381 tcttcacgtc gggcgttgct tcggcggacg gcgagggggc ggtctcggcc gaagcggtga
     1441 aggccgcgat ccgccagctg atcgatgcgg aagacccggg cgcgatcctc tccgacgatg
     1501 ccctggtcga tctgctcaag ggcaagggct tcgatctcgc ccgccgcacc gtcgccaagt
     1561 accgcgaggc catcggcctc ggctcctcgg tccagcggcg ccgccagaag gcgctggcag
     1621 gcgtccgcta gccggcgcca gaaggcgctg gcaggcgtcc gctagccgga tcattctctg
     1681 gttaattcaa cgagttaacg attcaccgca ccagcgcacg gttcagcgcg actcgcccac
     1741 actccgccgg aacgagtggt tgccgctccc gttaaccgtc cgcgcgaggg gaaccggccg
     1801 gggctgcgca tagcggcagc catgccgctt gcggaccggc gtcgcgaagg agaaacatcg
     1861 tgaaagcatc cgtactttgc ctcttggccg cggcttccct gccgctcggt gcctgctcat
     1921 cgatgagcaa tgaccacacc ctggccagcg ccggaaccgg cgccgcgatc ggcgcggcgg
     1981 gcggcgcggg cgtggctgcc ctcgccggcg gcgacgtgct ggccggcgcc gccatcggcg
     2041 ctgcggtcgg cgggatcgcc ggagcggtct gggccgaccg cgatggcgac ggccgcgtcg
     2101 acggctatgt ccgcgagggc cagtattatc cgggcgtgcc ccagggctat gatcccaatt
     2161 accagtctgc cgcagcggcg gcgcccgcct gccgctcggt cggcggcacc gcggtgaccg
     2221 gcgcggcggt cggcggcgtc ggcggcgccg ctgtcggggc cctggccggc aatgttctcg
     2281 ccggcgcggt gctgggcgcg gcggtcggcg gtcttgccgg tgccgtttgg gccgatcaca
     2341 acaatgacgg ctgcgtcgac ggctacgtgc gcgagggccg ctactacgaa ggcgcccccg
     2401 tgatccagcc gaccgacaac taccgccgca ccggggagcg cggctgatcc gatgagcagc
     2461 gctaccagcc cctccgcccg gcatccccgc taccgccggc tactggcgcc ggtagcgctg
     2521 gctctagccc tgggtctcgt gcccggggca cccgggccaa cgctcgccca ggcccaggca
     2581 gttgccccct cgatgcgcgc cgttctcgcc gaccgggcac cgggcgacct gcgcgccttc
     2641 tacgccgcgc gcggctggca gccgct
//
LOCUS       DATNBV010000270         2621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_174473,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000270 DATNBV010000000
VERSION     DATNBV010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174473"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VN627_06500"
     CDS             complement(<1..260)
                     /locus_tag="VN627_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXD02942.1"
                     /translation="MTEPMRIPDPLKQAFAVVATAASPQCRFDRALFVLGHMRCGSTA
                     LSRVLGNHPEISGQGEAHIAYRGRAALGQLLLAQMRRRSWKPG"
     gene            complement(257..1732)
                     /locus_tag="VN627_06505"
     CDS             complement(257..1732)
                     /locus_tag="VN627_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HXD02943.1"
                     /translation="MGGSSHRSGVLSGAVGFASRGLVQIMLFGVTIVATRVLSVAEFG
                     AYALGSLLLVASRQLFYVGPYEYLLKAPEQAGLPGACLAANLLQSGVLAGALALVWLA
                     TRLAFDEPMVSTILGLLIPSLFPVALTAWYEAMLLRGVRVRRYYVTTLVGDTAGALVA
                     VMLLLRGWGVTALIAQVYIRHLVLIVLYAVATGRAPSLAGRSGMAEVLRWSRARYAAV
                     GLTFTSTYGGDFVLGILLSPAATGVFRAANRIVAALADLFCQPLQKIAQTNLSASHAR
                     NQDTGTRWLTMLSGVGTIAWAGLVTLAFTARDLVPFALGAKWVPAVPVVVVFCLARGF
                     TLLDAVTTSFLVCHDRQRTMLKVQAITAVAVIALALAAAPFGATAVAMAVSLATTGMS
                     LTYGMLVVRLSRAGGDALLELVRTAAPPVVSVAAVLALLAWAFPNVTGGPSILLRLSA
                     AAGAFCLAAFAVRHRMLTAIGSLGHLPPVGPPATLAPVIGG"
     gene            complement(1736..>2621)
                     /locus_tag="VN627_06510"
     CDS             complement(1736..>2621)
                     /locus_tag="VN627_06510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXD02944.1"
                     /translation="DVRIAIIIATTGRASLLRRTVAQLARQSRRADRLIIVGVTSSDL
                     AGLHGPVCASAANEPIDLIHAERGLPRQRNAGLAHLAGSADIVVFFDDDFVPANDYLE
                     VLEREFSARPELVGATAQLIADGIKSPGISFDAAVALVENRRPTPPVERRLPALYGCN
                     LSIRLAAAEGLRFDEALPLYGWQEDVDFSYQLGRKGLLIYTSRLTGVHLGAKSGRTSG
                     LRLGYSQIANPVYLLRKKTIPRRLAWRLMLRNLAANVLRSAWPEPYVDRLGRLRGNLS
                     ALGDLVAGTLHPERILEL"
BASE COUNT          483 a          833 c          981 g          324 t
ORIGIN      
        1 cccggcttcc atgagcggcg gcgcatctgg gccaggagca gctggccaag cgcggcgcgg
       61 ccgcgatagg caatgtgagc ctcaccctgg ccgctgatct cgggatggtt gcccagcacc
      121 cgcgacagcg cggttgaccc gcagcgcatg tgaccgagca cgaagagggc gcgatcgaac
      181 cggcactgcg ggctggcggc cgtggcgacg accgcgaagg cctgcttgag tggatcggga
      241 atgcgcatgg gctcggtcac ccgccgatca ccggggcgag cgtcgcgggc gggccgacag
      301 ggggcaggtg tccgaggctg ccgattgccg tgagcatgcg atggcgcacc gcgaaggccg
      361 caaggcagaa ggcgccggcc gcagcgctca accgcagcag gatcgatggc ccgcccgtca
      421 cattggggaa ggcccaggcc agcagcgcga ggacggccgc cacggacacg acgggcgggg
      481 ccgccgtgcg aaccagttcg agcagggcgt cgcccccggc ccgggacagg cgcacgacca
      541 gcattccata ggtcaggctc atgccggtgg tggcgaggct tacggccatg gccacggcgg
      601 ttgccccgaa cggggcagcg gcgagagcga gagcgatgac ggccacggcg gtgatcgcct
      661 ggaccttgag catggtgcgc tgccgatcgt ggcagacgag gaaggaagtg gtgaccgcat
      721 cgagcagggt aaacccccgc gcgaggcaga acaccaccac cacgggcacg gccggcaccc
      781 acttggcgcc aagggcgaag gggaccagat cgcgcgcggt gaaggccagg gtaacgagcc
      841 cggcccaggc gatggtgccg acgccggaga gcatggtcag ccagcgggtg ccggtgtcct
      901 ggttgcgggc gtgggaggcg gaaaggttgg tctgcgcgat cttctggagc ggctggcaga
      961 acaggtcggc cagggcggcg acgatccggt tggcggcgcg aaacaccccg gttgccgctg
     1021 gcgacagcaa gatgccgagc acgaaatcgc cgccgtaggt cgaggtgaag gtcaacccca
     1081 cggcggcgta gcgggcgcgc gaccagcgca gaacttcggc catgccggac ctgccggcca
     1141 gtgacggcgc ccgtcccgtg gccacggcgt agagcacgat caggaccagg tggcggatat
     1201 agacctgggc gatcagagcg gtgacgcccc acccgcgcag caggagcatc accgcgacca
     1261 aggcgccggc cgtgtcgccc accagcgtgg tgacgtagta gcggcggacc cggacgccgc
     1321 gcagcagcat ggcctcgtac caggcggtga gcgcgacggg aaacaggctg gggatcagca
     1381 ggccgagaat ggtgctgacc atgggttcat cgaaagcaag gcgcgtggcc agccagacca
     1441 gcgcgagggc gccggccagc acgcccgact gcagcaggtt ggccgcgagg caggcacccg
     1501 gcagcccagc ctgctccggc gctttcagca ggtattcgta ggggccgaca tagaacagct
     1561 ggcgcgatgc gacgagcagc agcgagccca gggcataggc tccgaactcg gccaccgaga
     1621 gcacccgcgt ggcgacgatc gtgaccccga acagcatgat ctgcacaagg ccgcggctgg
     1681 caaagccgac tgccccgctg agcacgccgg aacgatgcga tgaaccgccc acgcctcaaa
     1741 gttccaggat gcgttcgggg tggagcgtgc cggcgacgag atcgcccagc gcggaaagat
     1801 taccgcgcag ccgacccagc cgatcgacat agggttccgg ccaggccgag cgcagcacgt
     1861 tggcggcaag gttgcgcagc atcagccgcc aggcaaggcg gcgggggatg gtcttcttgc
     1921 gcaggagata tacgggattg gcaatctggg aataacccag cctgagcccc gaagtgcgcc
     1981 cgctcttggc gccgagatga acccccgtca gccggctggt gtagatcagc aggcccttgc
     2041 ggccgagctg gtaggaaaaa tcgacatcct cctgccaccc atagagtggc agcgcctcgt
     2101 cgaagcggag gccttctgcc gcggcgaggc ggatgctgag attgcagcca tagagcgcgg
     2161 gcaggcggcg ttcgaccggg ggtgtggggc ggcggttttc caccagggcc acggcggcat
     2221 cgaacgagat gcccgggctc ttgatcccat cggcgatcag ctgcgccgtg gcgccgacaa
     2281 gttctggccg ggcggagaac tcgcgttcga gcacttccag gtagtcattc gcggggacga
     2341 aatcgtcatc gaagaagacg acgatatcgg cgctgcccgc aaggtgggcc agcccggcgt
     2401 tgcgctggcg cggcagcccg cgttcggcgt ggatcaggtc aatcggttca ttggctgcgc
     2461 tggcgcaaac ggggccatgc agcccggcaa ggtcggacga ggtgacgccg acgatgatca
     2521 gccgatcggc ccggcggctt tgcctggcga gctgcgccac ggtgcgccgc agcaggctgg
     2581 cccggccggt ggtggcgatg atgatcgcta tgcgcacgtc t
//
LOCUS       DATNBV010000271         3222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3658192,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000271 DATNBV010000000
VERSION     DATNBV010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3658192"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..2723
                     /gene="topA"
                     /locus_tag="VN627_06515"
     CDS             156..2723
                     /gene="topA"
                     /locus_tag="VN627_06515"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HXD02945.1"
                     /translation="MQLVIVESPAKAKTIEKYLGSGYKVLASYGHVRDLPVKDGSVRP
                     DEDFAMDWELYGDKQSRVRDITDAAKQADRLILATDPDREGEAISWHVRELLAKRKAL
                     PKEVERVTFNAITKDTILAAMKTPRELDQDLIDAYLARRALDYLFGFTLSPVLWRKLP
                     GAKSAGRVQSVALRLIVQREREIEVFVPQEYWSVVAKMEQGGTEFAARLVKFDGQKLE
                     RLSLGDAGMANRAKALVESASFRVEEVETRPTRRNPYPPFTTSTLQQEAARKLGFSAS
                     HTMRVAQTLYEAGAITYMRTDGVQMDPSAISAARAAISDRFSGHFLPEKPRHYETKAK
                     NAQEAHEAIRPTDFGKDRYGSADEARLYDLIYKRAMASQMASANIERTTVTFREGTGN
                     HELRATGQVVKFPGFLAVYEESHDASQDADEDESGLLPIMNKGDCPAKKGVDATQHFT
                     QPPPRYSEASLVKRLEELGIGRPSTYAATLQVLKDRNYVRTEKNRFFAEESGRLLTAF
                     LERFFERYVAYDFTAGMEEELDDVSGGREQWKALLAKFWKDFKPKSDEVMERKPSEVT
                     EALDEFLSDYLFPPQADGTDPRQCPKCGVGRLSLRGGRFGAFVACSNYPECKFTRKFA
                     QPGGAEGGDDEGLGKDPATGLEITRRAGRFGPYIQLGEGKEAKRASIPKDIPELDLEW
                     ALKLLSLPREVGNHPETGNPITASIGRYGPYLAHDGKYARLKTTAEVFDTGMNAAVVK
                     LAEAAANAGGRGNRQAAEPLKVFGKHPVSGGDIKLMAGRYGPYVTDGTTNATLPRDKK
                     PEDLTEAEAVVLIDERAAKGPAKGKKKGAAKKAAPAKKAATKKPAAKKSPAKKKDAE"
     gene            2720..2899
                     /locus_tag="VN627_06520"
     CDS             2720..2899
                     /locus_tag="VN627_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02946.1"
                     /translation="MSEKIARHKQPWKPEELQKLQVLAGKGKGLKEIAKALNRTEEST
                     KDAARQHGFAIAKLR"
     gene            complement(3072..>3222)
                     /locus_tag="VN627_06525"
     CDS             complement(3072..>3222)
                     /locus_tag="VN627_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HXD02947.1"
                     /translation="IVAYWSAYADPAFTRRLERTGFDVEEITVRARSNGKGPRHTIWF
                     GRRPN"
BASE COUNT          637 a         1085 c         1028 g          472 t
ORIGIN      
        1 cggtcaaggc cgccagtctg gccagtgagc ggctggtcca gctgatgacc ggcctgcgct
       61 gaaccgatcc gggcggatga acgcttgacg gggcgggcgg cgccccgtca ccctcgcgcg
      121 tgtacatacg cgtataggga ccgtttcaga tcgccatgca gctcgtcatc gtcgaatctc
      181 cggccaaggc caagaccatc gagaagtacc tcggctccgg ctacaaggtg ctcgcctcct
      241 acggccacgt ccgcgacctc ccggtcaagg acggctccgt ccggcccgac gaagacttcg
      301 cgatggactg ggagctctat ggcgacaagc agagccgcgt gcgcgacatc accgatgctg
      361 ccaagcaggc cgaccgcctg atcctcgcca ccgaccctga ccgcgagggt gaggcaatca
      421 gctggcacgt gcgcgagctg ctggcgaagc gcaaggccct ccccaaggag gtggagcggg
      481 tcaccttcaa tgcgatcacc aaggacacga tccttgccgc gatgaagacc ccgcgcgagc
      541 tcgaccagga cctgatcgac gcctacctcg cccgccgcgc gctcgattac ctgttcggct
      601 tcaccctctc gcccgtgctg tggcgcaagc tgcccggcgc gaagtcggcg ggccgagtgc
      661 agtcggtcgc cctgcgcctg atcgtgcagc gtgagcgtga gatcgaagtc ttcgtgccgc
      721 aggaatactg gtcggtcgtc gccaagatgg agcagggcgg aaccgagttt gccgcgcggc
      781 tggtcaagtt cgacgggcag aagctggagc ggctgagcct gggcgatgcc ggcatggcca
      841 atcgggccaa ggccctggtg gaaagcgcga gcttccgcgt ggaagaagtc gagacccggc
      901 cgacccgccg caatccctat ccgccgttca ccacctccac cctccagcag gaggctgccc
      961 gcaagctcgg cttctcggcc agccacacca tgcgcgtggc gcagacgctc tatgaggccg
     1021 gcgcgatcac ctacatgcgt accgacggtg tgcagatgga cccgagcgcc atctccgccg
     1081 cccgtgctgc catctctgac cgtttttccg ggcacttcct tcccgaaaag ccgcggcact
     1141 acgaaaccaa ggccaagaat gcgcaggagg cccacgaggc aatccgcccc accgatttcg
     1201 gcaaggatcg ctacggcagc gcggacgagg cgcggctcta tgacctgatc tacaagcgcg
     1261 ccatggccag tcagatggcc tcggccaata tcgagcgcac cacggtcacc ttccgcgaag
     1321 gcaccggcaa ccacgagctg cgcgccaccg ggcaggtggt gaagttcccc ggcttcctcg
     1381 ccgtctatga agaatcgcac gacgccagcc aggacgccga cgaggacgaa agcggactgc
     1441 tgccgatcat gaacaagggc gattgcccgg ccaagaaggg cgtggacgcc acccagcact
     1501 tcacccagcc gccgccgcgc tactcagaag ccagcctcgt caaacggctg gaggagctcg
     1561 gcatcggccg gccgtccact tatgccgcca ccctccaggt tctgaaggac cgcaactacg
     1621 tccgcaccga gaagaaccgc ttctttgcgg aagagagcgg gcggctgttg accgccttcc
     1681 tcgaacgctt cttcgagcgc tacgtcgcct acgatttcac cgccgggatg gaggaagagc
     1741 tcgacgacgt ttcgggcggg cgcgagcagt ggaaggcgct gctcgccaag ttctggaagg
     1801 acttcaagcc caagtccgac gaggtgatgg agcgcaagcc ctcggaagtg accgaggcgc
     1861 tggacgagtt cctttcggac tacctgtttc ccccccaggc ggacggcacc gatccgcgcc
     1921 agtgccccaa gtgcggcgta ggccggctca gcttgcgggg cgggcggttc ggagcgttcg
     1981 tcgcctgctc gaactatcct gagtgcaagt tcacccgcaa gtttgcccag ccgggcggtg
     2041 ccgaaggcgg agacgacgaa ggactgggca aggacccggc caccggcctg gagatcaccc
     2101 gccgcgctgg ccggttcggg ccctacatcc agttgggcga gggcaaggaa gccaagcgcg
     2161 cctcgatccc caaggacatc ccggagctcg atctcgaatg ggcgctcaag ctgctctcgc
     2221 tcccccgcga ggtgggcaat catccggaga cgggaaatcc gatcacggcg agcatcgggc
     2281 gctacgggcc ctatctcgcc catgacggca agtatgcccg cctcaagacc accgccgagg
     2341 tgttcgatac gggcatgaac gccgccgtgg tcaagcttgc ggaagctgcg gcaaacgctg
     2401 gcgggcgcgg caaccggcag gcagccgaac cgctcaaggt cttcggcaag cacccggtgt
     2461 cgggcggcga catcaagctg atggccgggc gttatggccc ctatgtgact gacggcacga
     2521 ccaacgccac cctcccgcgc gacaagaagc cggaagacct gaccgaagcc gaagccgtgg
     2581 tcctgatcga cgagcgcgct gccaaggggc cggccaaggg caagaagaag ggcgcggcca
     2641 agaaggctgc cccggcgaag aaggccgcga ccaagaaacc tgctgccaag aagtctccgg
     2701 ccaagaagaa ggacgcggaa tgagcgagaa gatcgcccgc cacaagcagc cgtggaagcc
     2761 ggaggaactg cagaagctcc aggtcctcgc cggcaagggc aaggggctga aggaaatcgc
     2821 caaggcgctc aaccggacgg aggaatcgac caaggatgcc gcccgtcaac atgggttcgc
     2881 catagccaag ttgcggtaac tataaactgc gacccagcgt ttcctgcatc gtcatcctga
     2941 acttgtttca gcatccatgg cgctcgggcg tcaccagcgc agcgtgccat tgctgacgcc
     3001 ggccttgccg aaatgtcgca tccatggatg ctgaaacaag ttcagcatga cgagcttgca
     3061 gtacgcgtct ctcagttcgg ccgccgaccg aaccagatcg tatgccgcgg gcccttgccg
     3121 ttgcttcgcg cccgcacggt gatttcctcc acgtcgaacc cggtccgctc cagccggcgg
     3181 gtgaacgccg gatcggcata ggccgaccag taggcgacga tc
//
LOCUS       DATNBV010000272         9202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2382003,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000272 DATNBV010000000
VERSION     DATNBV010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9202
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2382003"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..124
                     /locus_tag="VN627_06530"
     CDS             <1..124
                     /locus_tag="VN627_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HXD02948.1"
                     /translation="VSLPITALVNQFYAEVQAIGGGRQDTSALVRRLPLPGKGR"
     gene            136..1767
                     /locus_tag="VN627_06535"
     CDS             136..1767
                     /locus_tag="VN627_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD02949.1"
                     /translation="MPARTAAVLRCAAALLALAAATARADTLVDNVDGFTLDREGHVQ
                     RFTGLVIGNDGRVAQVLSQFDKRPARADYLLDGKGRVLMPGLVDAHAQVMKLGFAALT
                     LDLSPAKSLAEVQARLAAYAAAHPDRPWLLGRGWDPALVAGPTAAVLDAVVKDRPVWL
                     ESADGHQGWANSAALAAAGITGATKDPLGGRIVRLPPTPAAKAAPKAPPRASGLLVET
                     AMDLVARAVPAPRPEDRDLAFASAQDSLLKAGVTTVTDMGTTIEDWQAYRRAGDLGTL
                     RVRVLAFSAGTEAMTLIAGPAPTPWLYDDRLRLGGVALVLDGALATRGALLKSPYPAA
                     PRETGLPRLGETQLRNLMSRAGIDRFQVALEAHGPGAVAQGIAAFTELAQTYKGDRRW
                     RIDSAEAIDQADIPALAAGNITVVAQPGGLATGSLASAGLPLALGSGATGAERLSPWA
                     GIAAAATGPQPLPRSQALAAATTGAAWAAFADGRIGGLEPGQRADFVLLDRDPLQASP
                     DELRQTKVLETWVGGRKMWSAIESTSPSRMPATSR"
     gene            complement(1852..2652)
                     /gene="tatC"
                     /locus_tag="VN627_06540"
     CDS             complement(1852..2652)
                     /gene="tatC"
                     /locus_tag="VN627_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HXD02950.1"
                     /translation="MVFKVSDIDDSQAPLLDHLLELRTRLMRCILALGVAFGVCLYFA
                     QDILGFLLHPLAAAGQQKLVYTKLYGQFFVELKVALFAAFFVSFPVIANQIWAFVAPG
                     LYAKEKKAFLPFIWATPVLFTAGGALAYFVVMPTAFRWFLGFQGKQGGIQVEALPNSE
                     DYLGLVMQFILAFGISFLLPILLLLLNRAGMVTRAQLAGARRYVIVGITALAAVITPP
                     DVVSQLMLAIPLMLLFEGALIVMWFLDRKAAKEAAAEATVPVVVPPTS"
     gene            complement(2656..3090)
                     /gene="tatB"
                     /locus_tag="VN627_06545"
     CDS             complement(2656..3090)
                     /gene="tatB"
                     /locus_tag="VN627_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HXD02951.1"
                     /translation="MFDIGADELLLTAVVAIVVIGPKDMPRALRTAGRWVAKMRRMSS
                     AFRSGIENVIREAELEEMEAEWRKQNAAIMADHPPTAAEADALAAQAAAEGNPAALPP
                     PVVNAAEPVVPAATDEAAQAAAPVVSVEPPVPLRQNPVNPDT"
     gene            complement(3117..3383)
                     /locus_tag="VN627_06550"
     CDS             complement(3117..3383)
                     /locus_tag="VN627_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HXD02952.1"
                     /translation="MGFGSIGHWIILLVVVLILFGRGRISEIMGDFGKGIKSFKQGLN
                     DEDQARNVTPPAAQVPPAQIPPSAPPAAAPAEPAATQTTDTVQH"
     gene            complement(3468..4115)
                     /gene="scpB"
                     /locus_tag="VN627_06555"
     CDS             complement(3468..4115)
                     /gene="scpB"
                     /locus_tag="VN627_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HXD02953.1"
                     /translation="MSQSGSRPDDLVRAVEAALFAATEPMSAEAISAHLGGAEVKAAL
                     AELAAFYEGRGIALVERGKRWHFQTAPDLAHLLRREREEVRRLSRAATEVLAIVAYHE
                     PVSRAEIESIRGVQTAKGTLDVLLEAGWVRVAGRREVPGRPVIYATTPEFLAHFGLAS
                     RRDLPGIEELRAAGLLDPVEEALEEALAVRAEPSDPAGADDRAAETGNAPENDLD"
     gene            complement(4112..4921)
                     /locus_tag="VN627_06560"
     CDS             complement(4112..4921)
                     /locus_tag="VN627_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HXD02954.1"
                     /translation="MNLPPAQAAFEGDDWAGPAAAPSEDAALYLELDGWEGPLDLLLD
                     LARRQKVDLRKISILGLVDQYLAYIERAEALRLELAADYLVMAAWLAYLKSALLLPRE
                     EQEDPSPEELALRLQLRLQRLGAMREAAARLMGRDRLGRDVFRRGAPEGLRVERRNAW
                     TCAWFDLVQAYGQVKARTAPVVHMVRERPVMTLESALSRVAAMLGVSLDWMELRDFLP
                     PTAEGEWADPRLKRSALASSFVAALELARLGKAELAQDEVFGPLRLRGVAA"
     gene            complement(4918..5928)
                     /gene="nagZ"
                     /locus_tag="VN627_06565"
     CDS             complement(4918..5928)
                     /gene="nagZ"
                     /locus_tag="VN627_06565"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HXD02955.1"
                     /translation="MVPAIFGLSGLTLTADERAFFADADPAGYILFGRNVESPAQLRA
                     LTDELRAIRGRERLFICIDQEGGRVARMKPPTWPAYPAGEPFARLFDVAPASAIEAAR
                     SNAEALGLDLAEAGISVDCWPLLDVRQPGAHDVIGDRALGEEPRRVAALGRAVLDGLA
                     RAGVAGVVKHIPGHGRAMADSHKELPTVTADADELERDIAPFRALAAAPIAMTAHIRY
                     TAWDAERPATQSPYVIEEIIRGRIGFGGLLLSDDLDMEALAGTVPERAARSLAAGCDI
                     ALNCWAKMDDMVGIAKACPTMAEGAAGRLATALAVTGDLMADPARRAELAAKRDSLLA
                     LA"
     gene            complement(6015..6761)
                     /locus_tag="VN627_06570"
     CDS             complement(6015..6761)
                     /locus_tag="VN627_06570"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HXD02956.1"
                     /translation="MELLLFYGASAVLVAGLTLIERRRNPGATDWIINLQSWAVNLAI
                     GASVSHFWPRWVGGSLVEANDLPLWLSLPLFFVVRDFLEWFYHLCQHRIPALWAIHSL
                     HHSDPEMTALTTNRHFWADSLIKSLTIWSASGMIVQANDTLVAVYGGVSLYHYFVHAN
                     LKVDFGRFSWLLNCPAYHRRHHSRLPEHYDSNLAALFPIWDVLYGTYRRPDGWPPCGL
                     DEGGPGSLTELVTWPVRSKPDIAGPVTAEA"
     gene            complement(6823..7275)
                     /gene="rnhA"
                     /locus_tag="VN627_06575"
     CDS             complement(6823..7275)
                     /gene="rnhA"
                     /locus_tag="VN627_06575"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HXD02957.1"
                     /translation="MKHVEIFTDGACKGNPGPGGWGAILRLKTGDGREHEKELAGSER
                     QTTNNRMELMGAIEALRALREPCRVTLHTDSKYVIDGITKWIFGWQKNGWKNAAKKPV
                     ANAELWQDLVAAARPHQLEWVWVKGHDGHPENERADRLASDAADAARG"
     gene            complement(7272..8258)
                     /gene="thrB"
                     /locus_tag="VN627_06580"
     CDS             complement(7272..8258)
                     /gene="thrB"
                     /locus_tag="VN627_06580"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HXD02958.1"
                     /translation="MAVYTHLGAEELARLIGEFDVGPLRSAKGIAEGVSNSNWLIETA
                     GRDGRGARFILTMYEERTEVSELPFFLGLMDHLSARGCPVPATIHDREGQAYRTLNGK
                     ALALIEFLPGVSVSQPTERQARSVGAALARIHLSGGDFPMGRTNTLGLGGWRELIDGC
                     GAGGLESIDPVLAPLVAEEMGALMADWPADLPAGVIHADLFPDNVLMLGDEVTGLIDF
                     YFACTDLYAYDVAVTHAAWCFDATGARFDAALSTALLAGYEAVRPLSAAEHAALPLLA
                     RGAAMRFLSTRAYDWLHTPAGALVTRKDPLAFARRLEFYRGNPGIFGRGVFA"
     gene            complement(8281..9201)
                     /gene="ispH"
                     /locus_tag="VN627_06585"
                     /gene_synonym="lytB"
     CDS             complement(8281..9201)
                     /gene="ispH"
                     /locus_tag="VN627_06585"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HXD02959.1"
                     /translation="MIAAPRGFCAGVDRAIEIVERALERYGAPVYVRHEIVHNKFVVD
                     GLRAKGAVFVDELDQVPDDAPVIFSAHGVPKAVPAAATARGLEWLDATCPLVSKVHRQ
                     AERQIEAGRHILFIGHRGHPEVIGTLGQVSEGAITLVETEDDVARLDFPEGQPLAFLT
                     QTTLSVDDTANVIAALRARFPQIVGPRAEDICYATSNRQAAVKAIAAECQLVLVIGAP
                     NSSNSLRLVEVAERCGARAQLIQRADEIAPDWLDGVTTLGLTAGASAPEELVREVVAR
                     IAAWRQVNEETVVTAEEKMVFKLPRQLVDF"
BASE COUNT         1492 a         3417 c         2957 g         1336 t
ORIGIN      
        1 cgtctccttg ccgatcaccg cactggtcaa ccagttctat gccgaggtcc aggcgatcgg
       61 cggcgggcgg caggatacca gcgcgctggt ccggcgcctg ccgctgccgg ggaaaggccg
      121 atagggcacc aggccatgcc ggctcgtact gccgccgtcc tgcggtgcgc cgccgcgctg
      181 ctggccctcg cagccgcaac cgcccgggcc gacactctgg tcgacaatgt cgatgggttc
      241 acgcttgacc gcgaaggcca cgtccagcgc ttcaccggcc tcgtcatcgg caatgacggc
      301 cgcgtcgccc aggtcctcag ccagttcgac aagcgcccgg cccgggctga ctacctgctc
      361 gatggcaagg gccgcgtgct catgcccggc ctggtcgatg cccatgccca ggtgatgaaa
      421 ctcggctttg ccgcgctgac gctggacctg tcgccggcca aaagcctcgc cgaagtccag
      481 gcccgcctcg ccgcctatgc cgccgcccat cccgaccgcc cgtggcttct cgggcgcggc
      541 tgggatccgg cgctggtcgc cggtcccacc gccgccgtgc tcgacgccgt ggtgaaggac
      601 cgcccggtct ggctcgaatc tgccgacggc catcaaggct gggccaacag cgccgccctc
      661 gccgccgcgg ggatcaccgg cgccacgaag gaccccctcg gcggccggat cgtccgcctg
      721 ccgcccaccc ccgctgccaa ggccgcgccc aaggccccgc cgcgcgcgtc cggcctcctc
      781 gtcgaaaccg ccatggacct cgtcgcccgc gcggttcccg cgccgcgccc cgaagaccgc
      841 gacctggcct ttgcctccgc tcaggacagt ctgctcaagg ccggtgtaac cacggtcacc
      901 gacatgggca ccaccatcga ggactggcag gcctaccgcc gcgccggcga tctcggcacg
      961 ctcagggtgc gcgtcctcgc gttttccgcc gggaccgagg caatgaccct gatcgccgga
     1021 cccgcgccca cgccatggct ctacgacgac cgcctgcgcc tcggcggggt cgccctcgtg
     1081 ctcgacggcg cgctcgccac gcgcggcgcc ctgctcaaga gcccctatcc cgctgcgccc
     1141 cgcgaaacgg gcctgccgcg tctcggggaa acccagctgc gcaacctgat gagccgggcg
     1201 gggatcgacc gcttccaggt cgccctcgaa gcccacggcc ccggcgccgt cgcccaaggc
     1261 atcgccgcct tcaccgagct cgcccagacc tacaagggtg accgccgctg gcgcatcgac
     1321 agcgccgagg cgatcgatca ggccgacatc ccggccttgg cagcgggcaa catcaccgtt
     1381 gtcgcacagc caggggggct ggccacaggc tctctcgcca gcgccggcct gcccctcgcc
     1441 ctcggctcgg gcgcaaccgg agccgagcgc ctgtccccat gggcaggcat cgccgcagcg
     1501 gcaaccggcc cacaaccgct cccccgctcc caggccctcg ccgccgctac gacaggcgcc
     1561 gcatgggccg cattcgccga tggccgcatc ggcggcctcg aaccgggcca gcgcgccgat
     1621 ttcgtcctgc tcgaccgcga tcccctgcaa gccagccccg acgagctcag gcaaaccaaa
     1681 gtgctggaaa catgggtcgg cggaaggaag atgtggtcgg cgatagaatc aaccagccca
     1741 tccaggatgc cggccacatc gcgttgacat tgcccgttcg tgccgaggaa gggccgaagt
     1801 cgcagccgag ggcctgcctc gaagcacctg cgcagcacaa gaaccgcgaa ttcagctcgt
     1861 cggcggcacc actaccggca ccgtcgcctc agccgctgct tccttggctg ccttgcggtc
     1921 aaggaaccac atcacgatca gcgcgccctc gaacagcagc atcagcggga tcgccagcat
     1981 cagctgcgaa accacgtcgg gcggggtaat cacggcagcc agcgccgtaa tgccgacgat
     2041 cacatagcgc cgcgcgccgg ccagctgagc ccgcgtcacc attccggcgc ggttcagcag
     2101 cagcagcagg atcggcagga ggaagctgat cccgaaggcg aggatgaact gcatgaccag
     2161 gccaaggtag tcctccgagt tcggcagggc ttccacctgg atgccgccct gcttcccctg
     2221 gaatccaagg aaccagcgga aagccgttgg catgactacg aaataggcca gggccccgcc
     2281 cgccgtgaac agcaccggcg tcgcccagat gaaaggcagg aaggccttct tctccttggc
     2341 atagagcccg ggggcaacga aggcccagat ctggttggcg ataaccggga agctcacgaa
     2401 gaacgcggcg aacagcgcca ctttcaattc cacgaagaac tgcccgtaaa gctttgtata
     2461 gacgagcttc tgctgccccg ccgcagcaag cgggtgaagc aggaagccaa ggatgtcctg
     2521 cgcgaaatag aggcacacgc cgaaggcgac ccccagcgca aggatgcagc gcatcagccg
     2581 cgtgcgcagc tcgagcaggt ggtcgagcag cggggcctgg ctgtcgtcga tgtcggaaac
     2641 cttgaagacc atggtctagg tatccggatt cacaggattt tgccggagcg gcaccggcgg
     2701 ttccaccgaa accaccgggg ccgctgcttg ggcagcctcg tccgtcgcgg ccggcacgac
     2761 cggctcagcc gcattcacca ctggcggcgg cagcgcggcc ggattgccct ctgccgcggc
     2821 ctgggccgcc agcgcgtcgg cctcggcggc agtcggcggg tggtcggcca tgatcgcagc
     2881 gttctgcttg cgccactccg cctccatctc ctccagttcg gcctcgcgga tgacgttctc
     2941 gatgcccgag cggaaggcgc tcgacatgcg ccgcatcttc gccacccagc ggcctgccgt
     3001 gcgcagggcg cgcggcatgt ccttggggcc gatcaccaca atggcgacca ccgccgtcag
     3061 cagcagttcg tcggcgccga tatcgaacat ggcaggtcgc gacccttgtg cgcgggtcag
     3121 tgctggacgg tatcggtcgt ctgcgtggcg gccggttcag caggggcggc cgcgggcgga
     3181 gcgctcggcg gaatctgggc cgggggcacc tgggccgccg ggggggtcac gttgcgcgcc
     3241 tgatcctcgt cattaagacc ctgcttgaag cttttgatac ccttaccgaa atcacccatg
     3301 atttcggaaa tgcggccccg cccgaacagg atcagcacca ccaccagcag gatgatccag
     3361 tgcccgattg atccgaaacc catgtgcaaa aactcctgag gcgctgcgcc cgggcgccat
     3421 ggtcgctgcc atgagccaga acgacggcaa taggccccct tgccggttta atctaggtcg
     3481 ttttccggcg cattgccagt ctccgctgcc cggtcgtccg ccccggcggg gtcagacggt
     3541 tcggcgcgga cggccagcgc ctcttccagc gcctcctcga ccggatcgag caggccggcg
     3601 gcccgcaatt cctcgatccc gggcagatcc cgccgcgatg cgagcccgaa atgggcgagg
     3661 aattccggcg tcgtcgcata gatcaccggc cgccccggca cctcgcgccg ccccgccacg
     3721 cgcacccagc ccgcctccag cagcacgtcg agcgtaccct tggcggtctg cacgccgcgg
     3781 atcgattcga tctcggcccg gctgaccggc tcgtgatagg caacgatcgc cagcacttcg
     3841 gtggcggcgc gcgacaagcg ccgcacctcc tcgcgctcgc gccgcagcaa gtgggcgaga
     3901 tcgggcgcgg tctggaagtg ccagcgcttg ccccgctcga ccagcgcgat tccccgcccc
     3961 tcatagaacg ccgccagctc ggccagtgcc gccttcactt cggccccccc gagatgggcg
     4021 gaaatcgcct cggcactcat cggctcggtg gccgcgaaga gcgcggcctc gacggcgcgg
     4081 acaagatcgt ccggcctgga gcccgactgg ctcacgccgc cacgccccgc agccgcagcg
     4141 gcccgaacac ctcgtcctgc gccaactccg ccttgcccag ccgcgccagt tccagcgcag
     4201 ccacgaagct ggaagccagc gccgaacgct tgagccgggg atcggcccat tctccctccg
     4261 cggtcggcgg caggaaatcg cgcagctcca tccagtcgag gctcacgccg agcatggccg
     4321 ccacccgcga cagtgccgat tccagcgtca tcaccggccg ctcgcgcacc atgtggacca
     4381 cgggtgccgt ccgcgccttc acctgcccat aggcctgaac caggtcgaac cacgcgcagg
     4441 tccaggcgtt cctgcgttcc acccgcaggc cttccggcgc gccccggcgg aacacgtcgc
     4501 gccccagccg gtcacgcccc atcagccgcg ccgccgcctc gcgcatggcg cccagccgct
     4561 gcagccgcag ctgcaggcgc agggccagtt cctcggggct cgggtcttcc tgctcctcgc
     4621 gcggcagcag cagggccgat ttcaggtagg ccagccaggc cgccatcacg aggtagtccg
     4681 ccgccagttc cagccgcagc gcctcggccc gctcgatata ggcaaggtac tggtcgacga
     4741 ggcccagaat cgagatcttc ctcaggtcca ccttctgccg ccgggcaaga tccagcagca
     4801 ggtcgagcgg cccttcccac ccgtcaagct cgaggtagag cgcggcatcc tcgctcggcg
     4861 cagccgccgg ccctgcccag tcgtcgccct cgaaagcggc ttgagcgggc ggcaggttca
     4921 tgccagcgcc aacaggctgt cacgcttggc cgccagctcg gcccgccgcg ccggatcggc
     4981 catcaggtcg ccggtcaccg ccagcgctgt cgccagccgg cccgccgccc cttccgccat
     5041 tgtcggacag gccttggcga tccccaccat gtcgtccatc ttcgcccagc agttcagcgc
     5101 gatatcgcat ccggcggcga gcgaacgcgc ggcccgctcg ggcacggtgc cggccagcgc
     5161 ttccatgtcg aggtcgtcgg acagcagcag tccgccgaag ccgatccgcc cccggatgat
     5221 ctcctcgatc acatagggcg attgcgtcgc cggccgctcc gcgtcccagg cggtatagcg
     5281 gatatgggcg gtcatcgcga tcggcgcggc ggccagtgcc cggaagggcg cgatgtcgcg
     5341 ctccagctcg tccgcatcgg cggttacggt cggcagttcc ttgtggctgt cggccatggc
     5401 ccgcccgtgg ccggggatgt gcttgaccac gcccgccacc cccgcccgcg ccaacccgtc
     5461 gagcacggcg cggcccagcg ccgccacccg gcgcggctcc tcgcccagcg ctcggtcgcc
     5521 gatcacgtca tgggcgccgg gctggcgcac atcgagcagc ggccagcaat ccacgctgat
     5581 ccccgcctcg gcgaggtcga gcccgagcgc ctcggcattg ctccgcgccg cctcgatcgc
     5641 gctcgccggc gccacgtcga agaggcgggc gaaaggctcg cccgccggat aggccggcca
     5701 ggtgggcggt ttcatccggg ccacccggcc gccttcctga tcgatgcaga tgaacagccg
     5761 ctcgcgcccg cgaatggcgc gcagctcatc ggtcagcgcc cgcagctggg cggggctctc
     5821 cacattgcgg ccgaacagga tgtagcccgc cggatcggca tcggcgaaga aggcgcgctc
     5881 gtccgccgtc agcgtgagac cggaaaggcc gaaaatcgcc ggaaccatcc ccgctaactc
     5941 gcggaaaatc agggttcacg caagctcggg cgcggcaatc cgccggggat aaggcccggc
     6001 tcggcaagcc ccgatcaagc ctccgccgtg accggcccgg ctatgtccgg cttgctccga
     6061 accggccagg tgacaagttc ggtcaggctt cccggcccgc cttcatccag cccgcagggc
     6121 ggccagccat cgggccggcg ataggtgccg taaagcacgt cccagatcgg aaacagcgcg
     6181 gccaggttgc tgtcataatg ctcaggcagg cgggaatgat ggcggcggtg gtaggccgga
     6241 cagttgagca accacgaaaa ccgcccgaag tcgaccttga ggttggcgtg aacgaaatag
     6301 tgatacaggc tgacgccgcc atagaccgcc accagggtgt cgttggcttg gacgatcatg
     6361 cccgaggcgg accagatcgt cagcgacttg atcagcgaat ccgcccagaa gtgccggttg
     6421 gtggtcagcg cggtcatttc cggatcggaa tgatgcagcg aatggatcgc ccacagcgcc
     6481 ggaatgcggt gctggcacag gtggtagaac cattcgagga aatcgcgcac cacaaagaac
     6541 agcggcagcg aaagccacag cggcaggtcg ttcgcctcga ccagcgagcc tcccacccag
     6601 cgcggccaga aatggctgac gctcgcgcca atggcgaggt tcaccgccca cgactggaga
     6661 ttgatgatcc agtcggtcgc cccgggattg cgccggcgtt cgatcagggt caaacccgcg
     6721 acaaggaccg ccgaagcgcc atagaaaagc agcagttcca tggcggccat ctagccgggg
     6781 agtggttaac tcgaggttag ggggcgggcg actccgcccc ggtcagcccc gcgccgcatc
     6841 cgccgcgtcg ctggccagcc ggtcggcgcg ctcattctcc gggtggccgt cgtggccctt
     6901 gacccagacc cattcaagct ggtgcgggcg ggcggccgcg accaggtcct gccacagctc
     6961 ggcattggcg acgggcttct tggctgcgtt tttccaaccg tttttctgcc acccgaagat
     7021 ccacttggtg atcccgtcga tgacatattt gctgtcggtg tgcagcgtca cccggcacgg
     7081 ctcccgcagc gcccgcagcg cctcgatcgc gcccatcagt tccatgcggt tgttggtcgt
     7141 ctgccgctcg ctccccgcga gttccttctc gtgctcgcgg ccatcgcccg ttttcaggcg
     7201 caggatcgcg ccccagccgc ccgggccggg attgcccttg caggcgccgt cggtgaaaat
     7261 ctcgacgtgc ttcacgcgaa cacgccgcgg ccgaatattc ccggatttcc gcgataaaac
     7321 tccagccgcc gggcaaaggc cagcgggtcc ttgcgggtaa cgagggcgcc tgccggcgtg
     7381 tgcagccagt cgtaggcgcg ggtcgaaagg aagcgcatgg cggcgccgcg cgccagcagc
     7441 gggagcgcgg catgttcggc ggcagacagc ggccgcaccg cctcgtagcc ggccagcagc
     7501 gcggtcgaca gcgcggcatc gaaccgcgca ccggtcgcat cgaagcacca ggcggcatgg
     7561 gtcacggcga cgtcgtaggc atagagatcg gtgcaggcga aatagaagtc gatcagcccg
     7621 gtcacctcat cgcccagcat cagcacgttg tccgggaaca ggtcggcatg gatcacgccg
     7681 gcgggcaggt cggcgggcca gtcggccatc agcgcgccca tctcctccgc caccagcggc
     7741 gccagcacgg gatcgatcga ttccagcccc cccgccccgc agccgtcgat cagttcacgc
     7801 cagccgccaa ggcccagcgt attggtccgc cccatcggga agtcgccgcc ggacaagtga
     7861 atccgcgcca gtgccgcgcc caccgaccgc gcctgccgct ccgtcggctg gctgaccgag
     7921 acgccgggca ggaactcgat gagcgcgagc gccttgccat tgagagtacg gtatgcctgg
     7981 ccctcccggt cgtggatcgt cgccggcacc gggcagccgc gggccgaaag gtgatccatc
     8041 agcccgagga agaacggcag ctcggaaacc tccgtgcgct cttcgtacat ggtcaggatg
     8101 aagcgggcgc cgcgcccgtc gcggcccgcc gtctcgatca gccagttgct gttggaaacg
     8161 ccctcggcaa tgcccttggc actgcgcagc gggccaacat cgaactcgcc gatcaggcgc
     8221 gccagctctt cggcgccaag atgggtgtag acggccatag tctccctctc gtcccgccgt
     8281 tcagaagtcg accaactggc gaggcagctt gaacaccatc ttttcctcgg cggtcacgac
     8341 cgtttcctcg tttacctggc gccaggcggc gatccgggcg accacttcgc gcaccagttc
     8401 ctcgggcgcc gatgccccgg cggtcaaccc gagcgtggtc actccgtcaa gccagtccgg
     8461 cgcgatctcg tccgcgcgct ggatcagctg cgcccgggct ccgcagcgct cggccacttc
     8521 gaccagccgc agcgaattgg agctgttcgg cgcgccgatc accaggacca gctggcactc
     8581 ggccgcgatc gccttgaccg cggcctggcg gttcgaggtc gcatagcaga tgtcctccgc
     8641 gcgcggccca acgatctgcg gaaaccgcgc cctgagcgcc gcgatcacat tggccgtatc
     8701 gtcgaccgac agcgtcgtct gcgtgaggaa tgccagcggc tgcccctcgg ggaagtccag
     8761 ccgcgccacg tcgtcctcgg tctcgaccag ggtaatcgcg ccctcgctca cctggcccag
     8821 cgtaccgatc acctcgggat ggcccctgtg gccgatgaac aggatgtgcc gcccggcctc
     8881 gatctgccgc tcggcctggc ggtggacctt gctcaccagc gggcaggtcg cgtccagcca
     8941 ttcgagcccg cgcgccgtcg ccgccgccgg cacggccttg ggcacgccgt gggcgctgaa
     9001 gatcaccggg gcgtcgtccg gcacctggtc aagctcgtcg acgaacaccg cgcccttcgc
     9061 gcgcaggccg tcgaccacaa atttgttgtg aacgatctcg tggcgcacat agaccggtgc
     9121 gccatagcgt tcgagcgccc gctccacgat ctcgatcgcg cggtccacgc ctgcgcaaaa
     9181 cccgcgcggg gcggcgatca gc
//
LOCUS       DATNBV010000273         3516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2382372,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000273 DATNBV010000000
VERSION     DATNBV010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2382372"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VN627_06590"
     CDS             <1..319
                     /locus_tag="VN627_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02960.1"
                     /translation="EYGFAWAARIPAALPIYPRGHAQDAGGTDRDGCALRSVRFVTPV
                     PVAEVTAFYWAAARRAGLAAEHRMAGADHAVIARKGAATGVALIGQREDGLTQVDLVT
                     SGL"
     gene            complement(483..1895)
                     /locus_tag="VN627_06595"
     CDS             complement(483..1895)
                     /locus_tag="VN627_06595"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HXD02961.1"
                     /translation="MSAEIVSIAPATGEELWRGWPGDVEDCVERARRAWPSWAAQSLA
                     NRIELVRRTANELRKEADDLAALVANETGQPLWAARGEVDAVIERVELAVRAYAERTA
                     QRRLDNALQGTSALRHKPHGVVVVLGPASSPLAQPAAQIIPALIAGNAVIFKPSEKAP
                     AAAEVLVRCFHHAGLSANVIQYCPGGPETGQALALHEGVDGVLFTGSSHNGIALNRKL
                     AAVPNKIVALAMGGNNPIVAWDTPKLDDAAALIVQSAFSWAGQTCTNARRLIVRDTMF
                     DDLVGAVKAMADRLIVGAPFDDPAPFMGRVFDNATAEGLTESFVYLLSNGGRPIKHMV
                     RPVPGRPFLSPAMIEITGMAERPDVELLGPLLQVIRVGSLDEAITEANATRYGLVASL
                     IGGSPQDYNRFWANVRTGVVNWNRPTTAIAPGQPFGGTGLSGNHRPGGFYAADFSAYP
                     VASAEMEQPRLTIGLGLAEG"
     gene            complement(2024..3457)
                     /locus_tag="VN627_06600"
     CDS             complement(2024..3457)
                     /locus_tag="VN627_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HXD02962.1"
                     /translation="MAAEPQPAPYRPETAILELGDAFYDAVEPAEFPQSILRFRNDRW
                     APAVGLGALTDSEWIERFARFAPLQGNLPGPLALRYHGHQFQSYNPDIGDGRGFLYAQ
                     LRDRAGRLLDFGTKGSGQTPYSRFGDGRLTLKGAMREILATEMLEALGTYTSKTFSVI
                     ETGEALERGDEPSPTRSAVLVRLSHSHVRIGSFQRLAALGEKEHLAELVDYALREFPG
                     PLPPADAPGRDEPAVILLHQVVARLADMAASYMIAGFVHGVLNTDNMNVTGESFDYGP
                     WRWLPRHDPAFTAAYFDHSGLYAYGRQPGAIYWNCGQLAVALRPLAEAAPLIAALERF
                     APLYEEAMARRFCWRLGIAPQDTQADLALLLAAQEHMVAAELSPDALYFRHRGGRAAP
                     EGDFGELLGGREALPGAADDPLWAEAAPPTNHIEVVENLWAAIDQGDDWAPLHAHVGQ
                     IRALGVALGEPPQPAGHLWNLPEQNSP"
BASE COUNT          533 a         1205 c         1235 g          543 t
ORIGIN      
        1 cgaatatggc tttgcctggg ccgcccggat tcccgccgcc ctgccgatct acccgcgcgg
       61 ccatgcgcag gacgccggcg ggacggaccg cgacgggtgc gccctgcggt cggtccgctt
      121 cgtgaccccc gtgccggtgg ccgaagtcac cgcgttctac tgggccgccg cccgccgggc
      181 cggactcgcc gccgaacacc ggatggcggg cgccgaccac gcggtgatcg cccgcaaggg
      241 agccgccacc ggggtggccc tgatcggcca gcgcgaggac gggctgaccc aggtggatct
      301 cgtcaccagc ggcctgtaac tataccggac cttcttccgt ccgttcctcc cgagcttgtc
      361 gagggactat gccaagcgcc agctaggcgg aggcggtgcg ggctctcgct tcgctcgatc
      421 taatccctcg acaagctcag gaggatcggg attgaattac atggcccgtc ccggctcaac
      481 cctcagccct cggccaggcc cagcccgatg gtgagccgcg gctgctccat ttccgccgag
      541 gccaccggat aggcgctgaa atcagcggca tagaacccgc ccgggcggtg attgcccgaa
      601 agtccggtgc cgccgaacgg ctggcccggc gcgatcgccg ttgtcggccg gttccagttg
      661 accacgccgg tgcgcacatt ggcccagaag cgattgtagt cctgcggcga accgccgatc
      721 aggctggcga ccagcccata acgggtagca ttggcctcgg tgatcgcctc gtcgagcgag
      781 cccacgcgga tgacctggag caggggcccc agcaattcca catcgggccg ctcggccatg
      841 ccggtgatct cgatcatcgc gggcgagagg aacggccgcc ccggcaccgg gcggaccatg
      901 tgcttgatcg ggcgcccgcc gttagacagc aggtagacga agctctcggt cagtccctcg
      961 gccgtcgcat tgtcgaacac ccggcccatg aacggggcgg gatcgtcaaa cggggcaccc
     1021 acgatcagcc ggtccgccat ggctttcacc gcgcccacca gatcgtcgaa catggtgtcg
     1081 cgcacgatca ggcggcgggc attggtgcag gtttggccgg cccagctgaa agccgattgc
     1141 acgatcagcg cggcggcatc gtcaagcttg ggggtgtccc aggcgacgat cgggttgttg
     1201 ccccccatgg cgagcgccac gatcttgttc ggcacggcgg cgagcttgcg gttcagcgcg
     1261 atgccgttgt gcgaggagcc ggtgaacagc acgccgtcga ccccctcgtg gagcgccagc
     1321 gcctgtccgg tttccggccc gccggggcaa tactggatca cgttggccga cagaccggca
     1381 tgatggaagc agcgcaccag cacttcggcg gcggcgggcg ccttttcgct cggcttgaag
     1441 atcacggcat tgccggcgat cagcgcgggg atgatctgcg cggcgggctg cgcgaggggc
     1501 gaagaggccg ggccgagcac aaccaccacg ccatgcggct tgtggcgcag cgcgctcgtc
     1561 ccctgcaggg cattgtcgag cctgcgctgg gccgtgcgtt cggcataggc gcgcaccgcc
     1621 agttcgaccc gttcgatcac ggcgtcgacc tcgccccggg ccgcccacag gggctggccc
     1681 gtttcgttgg ccaccagggc ggcgaggtcg tcggcctctt tgcgcagttc gttggccgtg
     1741 cggcggacca gctcgatccg gttggcaagc gactgggcgg cccacgaggg ccaggcccgc
     1801 cgcgcgcgct ccacgcaatc ttccacatcg cccggccagc cgcgccacag ctcttctccc
     1861 gtggctgggg cgatggagac gatctctgcg ctcatcgggt gcggttcgcg ggtgcctgca
     1921 tgggtgtatg atcggatgcc tgatcggatt gccgcgcggg aagcggcact ccgcgcgtca
     1981 tcatagggcc ggtgcaaccg atgtcacaat cccatcgcct ccgttatggg gaattttgct
     2041 ccggaaggtt ccagaggtgc ccggccggct gcggcggctc gcccagggca acgcccaacg
     2101 cgcggatctg gccgacatgg gcgtgcagcg gggcccagtc gtcaccttgg tcgatcgccg
     2161 cccacaggtt ctcgaccacc tcgatgtgat tggtcggcgg cgctgcttcg gcccacagcg
     2221 gatcgtccgc cgcgcccggg agcgcctcgc ggccccccag cagctcgccg aagtctccct
     2281 ccggcgccgc gcggccgccg cgatggcgga agtagagcgc atcgggggag agttcggccg
     2341 ccaccatgtg ctcctgcgcg gccagcagca gcgcgaggtc ggcctgggtg tcctgagggg
     2401 caatccccag ccgccagcag aaccgccgcg ccatcgcttc ttcgtagagc ggcgcgaacc
     2461 gctccagcgc ggcgatcagc ggcgcagctt ccgccagcgg gcgcagcgcc acggccagct
     2521 ggccgcagtt ccagtagatc gcgccgggct ggcggccata ggcatagagc ccggaatggt
     2581 cgaaataggc agcggtaaag gcggggtcgt ggcgcggcag ccagcgccac gggccgtagt
     2641 cgaagctctc gccggtcacg ttcatgttgt cggtgttgag caccccgtgg acgaacccgg
     2701 cgatcatgta gctcgccgcc atgtcggcca gccgcgccac cacctggtgc agcaggatca
     2761 cggccggctc gtcgcgcccg ggcgcatcgg cggggggcag gggaccggga aactcgcgca
     2821 gggcatagtc gaccagttcg gccaggtgct ccttctcgcc cagggccgcc agccgctgga
     2881 agctgccgat ccggacatgg ctgtgggaca ggcgcaccag caccgccgag cgcgtcggcg
     2941 aaggttcgtc gccgcgctcc agcgcctcgc ccgtctcgat cacggagaat gtcttgctgg
     3001 tataggtgcc cagcgcctcc agcatttccg tcgccaggat ctcgcgcatg gcgcccttca
     3061 gcgtcaaccg cccgtcgccg aagcggctgt agggggtctg tcccgagccc ttggtgccga
     3121 aatcgagaag acgaccggcg cgatcacgca gctgggcata gaggaaaccg cgcccgtcgc
     3181 cgatatccgg attgtacgac tggaactggt gcccatgata gcgcagcgcc agcgggccgg
     3241 gcaggttgcc ctgcagcggc gcgaagcggg cgaaccgctc gatccattcg ctgtcggtca
     3301 gcgcgccaag gcctacggcg ggcgcccagc ggtcgttgcg gaagcgcagg atcgattgcg
     3361 ggaattcggc cggttcgacc gcatcgtaaa aggcgtcgcc aagctcgagg atcgccgttt
     3421 cggggcggta cggggcgggt tgcggttcgg ccgccatcag tgaatagggg gcaaggcgtg
     3481 aggggatggt gtcatggtcg cgtggtccga tggcta
//
LOCUS       DATNBV010000274         2394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1748408,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000274 DATNBV010000000
VERSION     DATNBV010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748408"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..554)
                     /locus_tag="VN627_06605"
     CDS             complement(72..554)
                     /locus_tag="VN627_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXD02963.1"
                     /translation="MLTYPPFELMAPAAPHLRLVANPPEDRKLQGPSGEPLRVLIVED
                     DAMVAATLADIVEESGGYVAGAMTSGLASVGAAAGTRPDVIVMDVGLPGMDGIDAAAI
                     IRSRSPMPIVFISGRDIREELTRRLGRDLSGVEILLKPIDRDPLCEAVLRALSRSTPG
                     "
     gene            746..1222
                     /locus_tag="VN627_06610"
     CDS             746..1222
                     /locus_tag="VN627_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXD02964.1"
                     /translation="MLIDQALVLIDQHIAAPNHSPTPSAEPDNQKRLVKNGRPLAVLI
                     VEDEALVGAVLSDAVEEFGGRVVGVVAAPTEAFGLVVERRPDVVIMDVRLKDGHDGLH
                     AADAMRLLHRTPIVFCTGYGNPETLRRIEKFGGAECLLKPVQPMQLCDAILRACGL"
     gene            complement(1314..1389)
                     /locus_tag="VN627_06615"
     tRNA            complement(1314..1389)
                     /locus_tag="VN627_06615"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1354..1356),aa:Ala,seq:cgc)
     gene            1577..1867
                     /locus_tag="VN627_06620"
     CDS             1577..1867
                     /locus_tag="VN627_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HXD02965.1"
                     /translation="MYALFNLIATVISIYIYVLVASAILSWLVAFNVLNTSNRFVYMV
                     GDFLYRITEPALRPIRRFVPSLGGVDISPVVLILLLVFLRDFLLIDLARAML"
     gene            1884..2213
                     /locus_tag="VN627_06625"
     CDS             1884..2213
                     /locus_tag="VN627_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HXD02966.1"
                     /translation="MALPLAPTADGVIVTIKLTPKARSSGIDGVVEEPGPEGPRSLLK
                     LRVTEAPESGKANAAMIALLAKAWRRPKRDFTLIAGETSRLKRVHVAGAPETLLRDLS
                     SRLQGEP"
     gene            2210..>2394
                     /locus_tag="VN627_06630"
     CDS             2210..>2394
                     /locus_tag="VN627_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HXD02967.1"
                     /translation="MSNIIDGKAFADRLAQRYAGEVAKMKEKHGITPGLAVVLVGENP
                     ASQVYVRNKTNQTAVAG"
BASE COUNT          435 a          807 c          750 g          402 t
ORIGIN      
        1 gcacggcctg agcgagcatc ggagagccac atcgcgacac cgccggcgaa gtcaccggag
       61 ccggcggtgc ttcaccccgg cgtagatcga gaaagggccc gtagaacggc ttcgcagagc
      121 gggtccctgt cgatgggctt cagcagtatt tcgaccccgg acagatcgcg ccccaaacga
      181 cgcgtgagct cctctcggat gtcgcgaccg ctgatgaaca cgatcggcat cggagagcgc
      241 gaacggatga tcgccgcagc gtcaattccg tccatgccgg gcaacccgac atccatgaca
      301 atcacgtcgg gccgcgtgcc cgctgcggcc cccacggagg ccaagccgga cgtcatcgcc
      361 ccggcaacgt acccgccact ttcttcaaca atgtccgcga gcgtcgcggc gaccattgcg
      421 tcgtcctcaa cgatcagcac gcgcaagggc tcgcccgacg ggccttgaag tttcctgtct
      481 tcgggtggat tagcaacgag gcggagatgc ggcgcggcgg gtgccatcag ctcgaacggc
      541 gggtaggtca acatgagtgc gcgccctttg cgaattacgc cccaaagaat gccactgcga
      601 ccgccggaga gtaagcaagc attcgaggtc ctgcaggatc gcacggccag ccgtgtgtac
      661 aaaatgcaca ctgcgcgcca ttactttctt accttaactc actgaattag ttgctttttg
      721 gcaggttcga ccatagcatc agccgatgct tatcgaccaa gcgctcgtgc tcatcgacca
      781 acacatcgct gcgccaaatc actcgccgac tccatcggct gaaccggaca atcagaaacg
      841 gcttgtcaaa aatggtcgac cgctcgccgt cctgatcgtc gaggacgagg cactggtggg
      901 tgcggttctc tcggacgcgg tcgaggagtt tggcggtcgc gtggtcgggg tcgtcgcggc
      961 accgactgaa gccttcgggc tcgtcgtcga gcgtcgtccg gacgttgtga tcatggatgt
     1021 tcggctgaag gacggtcacg atgggctgca cgcggccgat gcgatgcggc tcctgcatcg
     1081 cacgcccatc gtgttctgca ccggctacgg caaccccgag acccttcgca ggatcgagaa
     1141 attcggcggg gccgagtgtc tgctgaagcc tgtccaaccc atgcagcttt gcgacgccat
     1201 cctgagggcg tgcggcctct gaccgacact ggcggcaaga ggcgtggcgg ccccgtgcag
     1261 cgtcctcacg cagcggacac ggactgtctc agctaagcga ttaccggcgg gcttggtgga
     1321 gccgagggga atcgaacccc tgacctcctc attgcgaacg aggcgctctc ccatctgagc
     1381 tacggccccg ccgggtctgc tggcggcgcg gataatgggt atgggggctg ccggtgtcaa
     1441 gaccgctcag gccccgtttg cgcccccgga aaaccccggg aaaccgcgca gggacaaagc
     1501 cttgcaggga atccgacacg tcgtgtaggg tccgcggcac cgggccctac ggccccgccg
     1561 ggtcagacga ggcaagatgt acgcgctgtt caatctgatc gccacggtca tcagcatcta
     1621 catctatgtg ctggtcgcat cggccatcct gtcctggctg gtggccttca acgtactgaa
     1681 caccagcaac cgcttcgtct acatggtcgg cgacttcctc taccgcatca ccgagccggc
     1741 gctgcggccg atccgccgct tcgtgccgtc gctgggcggc gtcgacatct cgccggtcgt
     1801 gctgatcctg ctgctggtct tcctgcgcga cttcctgctg atcgacctcg cccgggcgat
     1861 gctctgacgc cgcgcggccc agtttggccc tgccgctcgc cccgaccgct gacggcgtga
     1921 tcgtgaccat caagctgacc cccaaggcgc gcagttcagg catcgacggc gtcgtcgagg
     1981 agcccggccc ggaggggccg cgcagcctgc tcaagctgcg ggtcaccgag gcgccggaga
     2041 gcggcaaggc caacgccgcc atgatcgccc tgctcgccaa ggcctggcgc cggcccaagc
     2101 gcgatttcac cctcatcgcc ggcgagacca gccggctgaa acgcgtccat gttgcgggcg
     2161 caccagaaac actgctgcgc gacctttcgt cccgtctcca gggggaacca tgagcaacat
     2221 catcgacggc aaggccttcg ccgaccgcct cgcccagcgc tacgccggcg aagtcgcgaa
     2281 gatgaaggag aagcacggga tcacgcccgg cctcgcggtc gtgctggtgg gcgagaatcc
     2341 ggccagccag gtctatgtac gcaacaagac caaccagacc gcggtcgccg gcat
//
LOCUS       DATNBV010000275         3602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_178249,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000275 DATNBV010000000
VERSION     DATNBV010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178249"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..414)
                     /locus_tag="VN627_06635"
     CDS             complement(172..414)
                     /locus_tag="VN627_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HXD02968.1"
                     /translation="MRILADLPDDDIRWLDELAAERGKSRAALLREAVSAFRAGERDW
                     LEQGFGLWTRYGRGADGAAYEQALRAEWTGPAGETT"
     gene            complement(637..2013)
                     /gene="glmU"
                     /locus_tag="VN627_06640"
     CDS             complement(637..2013)
                     /gene="glmU"
                     /locus_tag="VN627_06640"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HXD02969.1"
                     /translation="MTGSSPAAPLAVVTLAAGKGTRMKSDLHKVLHPIAGRPMIEHLL
                     ASVAELAPERQVVVVGSGREQLEKQLGGRAAIAVQEPQLGTGHAVQQAEGALAGFSGD
                     VLILYGDVPFVSAETMRAMLDRLHAPDAPEVVVLGFEPAETLQYGRVLAHADGRIAKM
                     VEHKDASEAERACRLCNSGLMAVKAGRLFDLLGKVGNANAQGEYYLVDIVNIANAEGG
                     HCAVVVTDDEGEVAGINSRGELAEAEARWQAARRVRAMAEGATLIAPETVWFAWDTQL
                     GRDVTIEPNVVFGPGVRVADGVTIHAFTSLAGAALESGVEVGPYARLRPGAVLKAGSK
                     VGNFVEVKNAVLGEGAKASHLTYLGDADIGAGANIGAGTITCNYDGYFKYRTVIGERA
                     FIGSNSSLIAPVRIGADAIVAAGSAVSRDVDDGELRLVRAEQLVKPGWADRFHDAMKK
                     KKAAAKKG"
     gene            2097..2798
                     /locus_tag="VN627_06645"
     CDS             2097..2798
                     /locus_tag="VN627_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXD02970.1"
                     /translation="MTDFPFRIVGFDLDGTLLDSGDDLGVALNHVLARLGRETVPLAE
                     VRGLIGGGAKMLLERGLQRTGGLLPADEMRALHHELIDFYADNIAHHTRPFPGTEAML
                     DDLAARGVAVAVVTNKMERLAVALLDALNLSNRFATIIGGDTLGPGRSKPAPDLLLEM
                     TRRLGEGPAAYVGDTTYDTRAAAAAGMPCVAVSFGFNDAPVATLGAAAVIDHFDALVP
                     ALTSLAPARSLNEAC"
     gene            complement(2782..3267)
                     /locus_tag="VN627_06650"
     CDS             complement(2782..3267)
                     /locus_tag="VN627_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD02971.1"
                     /translation="MSETTNAPIDRLTRLEVMVACRDLSDRYALAVNSNDVDAFVALF
                     TDDAIWERPAMALNGPGEIRAFIAGQFSQARQVRHLNGHCVVDVIDADTGQVRSATTV
                     YEMADTGEGVTNLPPPALIAEYETTMVRRGEAWKIRRHKAVVRFVSEHSLGLPGVSKP
                     R"
     gene            complement(3322..>3602)
                     /locus_tag="VN627_06655"
     CDS             complement(3322..>3602)
                     /locus_tag="VN627_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HXD02972.1"
                     /translation="KRFDAMAVYRPMWLASRTDAFSQTGVVDKAGASGRFAGHSVDLR
                     ARYWLVPGLLRGEVDYDWIGKGRFLRDAPNAPHTGDTHYASFTLTASL"
BASE COUNT          568 a         1353 c         1089 g          592 t
ORIGIN      
        1 tggcaaggaa ccgctcggtc tcgtcacggc aggcagccgg aacctgggcc atgacctcaa
       61 gccaggtaat ccggctgatc cacggacgct tggcccggcg cagctcggcc atggcctggt
      121 gatggcctga gagagtgtcg acaatcaggc cggtgtcgaa acaggtgtcg gtcacgtggt
      181 ctcacctgcc ggcccggtcc attccgcccg taacgcctgc tcgtaggccg cgccgtcagc
      241 gccccggcca tagcgggtcc acaagccgaa gccctgctcc agccagtccc gctccccggc
      301 gcgaaaggcg ctgaccgcct ctcgcagcag ggccgcgcgg ctcttgccgc gctcggccgc
      361 cagttcgtcg agccagcgga tatcgtcgtc aggaagatcg gcgagaatgc gcataatgat
      421 atatagatat catatcgtga tatggaatgc aaggagttcg tgccctcgga cgaacccgcc
      481 tatgccgcaa cgcgcacctc tgccgtatgg ccccaaggtg aactttgcgt cacccgagga
      541 taggggaatg tgcctaaccg tcatcccggg cttgacccgg gccaggcctg aaggtcggcc
      601 accggttccg gatcaagtcc gggacgacgc tggccctcac cccttcttcg ccgccgcttt
      661 cttcttcttc atcgcatcgt gaaaccggtc cgcccagccg ggcttcacca gctgctcggc
      721 ccgcaccagc cgcagttcgc catcgtccac gtcgcggctc accgcgctcc cggcagccac
      781 gatggcatct gccccgatcc gcaccggcgc gatcagcgac gagttggagc cgataaaggc
      841 tcgctcgccg atcaccgtgc ggtacttgaa atagccatca taattgcagg tgatcgtccc
      901 cgcgccgata ttcgcccccg cgccgatatc ggcatcgccc agataggtca ggtggctggc
      961 cttggccccc tcgcccagaa ccgcattctt gacctcgacg aagttgccga ccttcgaccc
     1021 tgccttgagc accgcgcccg gccgcagccg cgcatagggg ccgacttcca cgccgctctc
     1081 cagcgccgcc ccggccaggc tggtgaaggc atggatggtc acgccatcgg cgacccgcac
     1141 gcccggcccg aagaccacgt tcggctcgat cgtcacgtcg cggcccagct gggtatccca
     1201 ggcgaaccac accgtctcgg gcgcgatcag cgtcgccccc tcggccatgg cccgcacccg
     1261 ccgcgccgcc tgccagcgtg cttccgcctc ggccagttcg ccccggctgt tgatcccggc
     1321 gacctcgccc tcgtcatcgg tcaccaccac ggcgcagtgc ccgccctcgg cattggcgat
     1381 gttgacgatg tcgaccaggt aatactcgcc ctgggcattg gcattgccaa ccttgcccag
     1441 caggtcgaac agccgcccgg ccttgaccgc catcagcccc gaattgcaca gccggcaggc
     1501 ccgctcggcc tcgctggcgt ccttgtgctc gaccatcttg gcgatgcggc catcggcatg
     1561 ggccagcacc cggccatatt gcagcgtttc cgccggttcg aagccgagca ccaccacctc
     1621 cggcgcgtcc ggcgcatgaa gccggtcgag catggcgcgc atcgtctcgg cgctcacgaa
     1681 gggcacgtcg ccataaagga tcagcacgtc gcctgagaac ccggccagcg ccccctcggc
     1741 ctgctgcacg gcatggccgg tgcccagctg cggctcctgc acggcaatcg ccgcccgtcc
     1801 gcccagctgc ttttccagct gctcacggcc cgagccgacc accaccacct gccgctcggg
     1861 cgccagttcg gccacgctgg ccagcaggtg ttcgatcatc ggtctgccgg cgatcgggtg
     1921 gaggaccttg tgcaggtcgc tcttcatccg ggtgcccttg ccggcggcaa gggtgacaac
     1981 ggcaagcgga gcagcggggg aggaaccagt catgcgggcc tctctgccag catttcaacg
     2041 cagattccat caccggtaga ttgcgaattg gtcacctttg cgccacggcg ccgcccatga
     2101 ccgattttcc cttccgcatc gtcggcttcg atctcgacgg taccctgctc gattccggcg
     2161 acgatctcgg cgtcgcgctc aaccacgtgc tggcccggct cggccgcgaa accgttcccc
     2221 tcgccgaggt gcgcggcctg atcggcggcg gtgcgaagat gctgctggag cgcgggctgc
     2281 aacggaccgg cggcctgctc ccggcggacg aaatgcgcgc cctccaccac gagctcatcg
     2341 atttctacgc cgacaacatc gcccaccaca cccgcccctt ccccggcacc gaagccatgc
     2401 tcgatgacct ggcggcccgc ggggttgcgg tcgcggtcgt caccaacaag atggagcgcc
     2461 tcgccgtcgc cctgctcgac gcgctgaacc tgtcgaaccg attcgccacg atcatcggcg
     2521 gcgataccct cgggcccggc cgctccaagc ccgcgcccga cctgctgctg gaaatgaccc
     2581 ggcggctcgg cgaaggaccc gcggcctatg tcggcgatac cacctacgac acccgcgccg
     2641 ccgccgccgc cgggatgccc tgcgtcgcgg tcagcttcgg cttcaacgat gcgcctgtcg
     2701 ccaccctcgg cgccgccgcc gtgatcgacc atttcgacgc gctggtgccc gccctcacca
     2761 gcctggcccc cgcccgcagc ctcaacgagg cttgctgacc cccggcaagc ccagcgagtg
     2821 ctccgacacg aagcggacga ccgccttgtg gcggcggatc ttccatgcct cgccccggcg
     2881 caccatggtc gtctcgtact cggcgatcag cgcgggcggc ggcaggttcg tcaccccctc
     2941 gcccgtgtcg gccatctcat agactgtcgt ggccgaacgc acctgcccgg tatcggcgtc
     3001 gatcacgtcg acgacgcagt gcccgttgag atggcgaacc tggcgtgcct ggctgaactg
     3061 cccggcgatg aaagcccgga tttcgcccgg tccgttcagc gccattgccg gtcgttccca
     3121 gattgcatcg tcggtgaaca gggccacgaa ggcgtccacg tcattgctgt tgacggccag
     3181 ggcatagcgg tcgctcaggt cgcggcaggc gaccattacc tccagccgcg tcagccggtc
     3241 aatcggcgca ttcgtggtct cgctcatgcc agtcctctct tttcgcgatg acgacaggtt
     3301 ggcgggcgcg aagccggaaa actagagcga agcggtcagc gtgaagctgg catagtgggt
     3361 atcaccagtg tgcggcgcat tgggcgcatc gcgcaggaac ctgcccttgc cgatccagtc
     3421 gtagtccacc tcgccccgca gcagccccgg caccagccag taccgcgccc gaaggtctac
     3481 gctgtgcccg gcaaagcgtc ccgaggcgcc cgccttgtcc accaccccgg tctgcgaaaa
     3541 ggcatcggtg cggctggcca gccacatcgg ccggtagacc gccatggcgt cgaaccgctt
     3601 gc
//
LOCUS       DATNBV010000276         2346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2723034,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000276 DATNBV010000000
VERSION     DATNBV010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2723034"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /gene="flgF"
                     /locus_tag="VN627_06660"
     CDS             <1..721
                     /gene="flgF"
                     /locus_tag="VN627_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HXD02973.1"
                     /translation="FSGLNASMVRQRVIASNMANAQTIGFRADTLQFTPMTVKDGESL
                     EVRALSDGEVRGADMHEGTLTQTGKPLDVAMVGKALLTVQAADGTESYTRRGDLSVSP
                     TGALVNGDGAPVIGDNGPISIPPGGQVSIAEDGAVLVRDPATPDAPPTKIDRIKLASA
                     AGTRIEKGLDGLLRVYGGGALPADLEAKVIPGALEQSNVQPSDVLVQMVEAQRLFDIR
                     TKLISTAKDLDEGGAALMRLS"
     gene            735..1523
                     /gene="flgG"
                     /locus_tag="VN627_06665"
     CDS             735..1523
                     /gene="flgG"
                     /locus_tag="VN627_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HXD02974.1"
                     /translation="MPSSALHVARTGLEAQDQRMRVIANNLANVGTTGFKRDRANFAT
                     LAYQDARVAGQQSSAETAYATGLNLGTGVGIQSTSRIDTQGTLQTTGNALDLALDGDG
                     FFQVQMPGGQLAYTRAGNFTRSADGQLVTAQGYPLQPAITVPEGASAITIAADGTVSA
                     QLPGQSDPTQLGQITLASFTNPAGLKAMGDNFLGETAASGQAQLGPAGQNGRGNIRQG
                     FLEASNVNVVEELVDMIECQRAYEINSKMISAVDEMLKNANQTL"
     gene            1541..2344
                     /locus_tag="VN627_06670"
     CDS             1541..2344
                     /locus_tag="VN627_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HXD02975.1"
                     /translation="MTDHQSSHLTLPRTATAGPAPASLAIVLPRRPLRRRKPGVRLAR
                     LSFTAPAAALSLAAFALSAPGNAAKPRAGFEASLPMTTAPATVRPATGAIFDATMGYA
                     SLVQGTLARRVGDPLTILLEENTTTSKSAGSKTSRNGGFSLTPPSAGPLSFLNPDALK
                     ASGQSSFKGDGTATQTSSFSGTVGVTIAEVRPNGTALVKGEKRLLLSQGQEWIQFSGI
                     VRLSDIDAQNTVRSSQVADARMEYAGNGAIQRAGREGWLSRFFNMISPF"
BASE COUNT          416 a          872 c          729 g          329 t
ORIGIN      
        1 cttctccggc ctcaacgcct cgatggtgcg ccagcgggtg atcgccagca acatggccaa
       61 tgcccagacc atcggcttcc gcgccgatac gcttcagttc accccgatga ccgtgaagga
      121 cggtgagagc ctcgaagtgc gcgccctgtc agatggcgag gtgcgcgggg ccgatatgca
      181 cgagggcacg ctgacgcaga ccggaaagcc gttggacgtg gccatggtcg gcaaggccct
      241 gctcacggtc caggccgcgg acgggacgga aagctatacc cgccgcggcg acctttcggt
      301 ttcacccacc ggcgcgctgg tcaatggcga cggcgccccg gtgatcggcg acaatggccc
      361 gatctcgatc ccgccgggcg gccaggtcag catcgccgaa gacggcgccg tgctggtccg
      421 cgatccggcc acgcccgacg cgccgcccac aaagatcgac cggatcaagc tcgccagcgc
      481 ggccggaacc cgcatcgaaa agggcctcga cggcctgctg cgggtctacg gcggcggcgc
      541 cctgcccgcc gatctcgagg ccaaggtcat tcccggcgcg ctcgaacaat ccaacgtcca
      601 gccgagcgac gtcctcgtcc agatggtcga ggcccagcgc ctgttcgaca tccgcaccaa
      661 gctgatcagc accgccaagg acctcgatga aggcggcgcg gcgctgatgc gtctttcctg
      721 agaggtaacg agccatgccc agttccgccc ttcacgtcgc ccgcaccggt ctcgaggccc
      781 aggaccagcg gatgcgcgtg atcgccaaca acctcgccaa tgtcggcacc accgggttca
      841 agcgcgaccg ggccaatttc gcgacgctcg cctatcagga tgcgcgcgtc gccggccagc
      901 agagctcggc cgagaccgcc tatgccaccg ggctcaacct cggcacgggc gtaggcatcc
      961 agtcgaccag ccgcatcgat acgcagggca ccctgcagac caccggcaac gcgctcgacc
     1021 tcgcgcttga tggcgatggc ttcttccagg tgcagatgcc gggcggccag ctagcctata
     1081 cccgcgcggg caatttcacc cgctcggcgg atggccagct ggtgaccgcc cagggctatc
     1141 cgctccagcc ggcgatcacc gtgcccgagg gcgccagcgc gatcacgatc gccgccgacg
     1201 gcaccgtttc cgcccagctg cccggccagt ccgatccaac gcagctcggc cagatcaccc
     1261 tcgccagctt caccaatccg gccggtctca aggccatggg ggacaacttt ctcggggaaa
     1321 ccgccgcttc gggccaggcc cagctcggcc ctgccggaca gaacggacgc ggcaacatcc
     1381 gccagggctt tctcgaagcc tccaacgtca acgtcgtcga ggagctggtc gacatgatcg
     1441 aatgccagcg cgcctacgag atcaattcca agatgatctc ggccgtcgac gagatgctca
     1501 agaacgccaa ccagaccctg tgatccggga gcccgccaca atgaccgacc atcagtcctc
     1561 ccacctcacc ctgccgcgca cggcgaccgc cgggccggcc ccggcatcgc tcgccatagt
     1621 cctgccgcgc cggccactac gacgccgcaa gcccggcgtg cggctggcgc gcctgagctt
     1681 caccgctccg gccgctgcgc tcagcctcgc cgccttcgcg ctcagcgcgc ccggcaatgc
     1741 cgccaagccg cgcgctgggt tcgaggccag cctgccgatg accaccgccc cggccacggt
     1801 ccgcccggcc accggcgcga tcttcgatgc gacaatgggc tatgccagcc tggtgcaggg
     1861 cacgctggcc cggcgggtcg gcgatccact gacgatcctg cttgaggaaa acaccacgac
     1921 cagcaagtcg gcgggctcga aaaccagccg caacggcggc ttctcgctga ccccgcccag
     1981 cgccggaccg ctgtcgttcc tcaaccccga tgcccttaaa gcctcgggcc agtcgtcgtt
     2041 caagggagac ggtacggcga cgcagaccag ttccttcagc ggcacggtcg gcgtcaccat
     2101 cgccgaagtg cgccccaacg gcacggcgct ggtcaaagga gaaaagcgct tgttgctgag
     2161 ccagggccag gaatggatcc agttctccgg catcgtgcgc ctgtccgaca tcgacgcgca
     2221 gaacacggtg cgctccagcc aggtcgccga tgcgcggatg gaatatgccg gaaacggcgc
     2281 catccagcgc gccgggcgcg aggggtggct gagccggttc ttcaacatga tctcgccgtt
     2341 ctgatg
//
LOCUS       DATNBV010000277         5498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_178567,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000277 DATNBV010000000
VERSION     DATNBV010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178567"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VN627_06675"
     CDS             complement(<1..724)
                     /locus_tag="VN627_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXD02976.1"
                     /translation="MVSIATWNINSVRLRADQVVRFLEQEQPDVLCLQEIKCAEHLFP
                     HEVFEAAGYTHRVVHGQKGYHGVATVSRIPLREISRHDWQDNGEARHIGVELLGPGQG
                     VVLENVYIPAGGDIPDREANPKFGQKLDFLGRMTRWAEQLDRPTLIVGDFNIAPLDCD
                     VYDHKALLKVVSHTPLEVETLQRFADAHGWVDLGRKHIPAPERNWTWWSYRTFWQEKD
                     RGRRLDHMWASPELAAQSTGHRL"
     gene            complement(1227..1886)
                     /locus_tag="VN627_06680"
     CDS             complement(1227..1886)
                     /locus_tag="VN627_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HXD02977.1"
                     /translation="MNTLIGIFRKSLGTAVSAALVVSAPLIAAGLTTPAVAQTANDLD
                     QAVDALRAITTMQADFLQTDRNGQRVAGKLTIKRPGKIRFQYQQGVPMLIVGDGKALT
                     MVDYEVRQVQRWPIGNSPLGALLDPTRDVNRFGTLQPATDPGIVSIEVRDRKHPEYGV
                     ITLIFARKAGAPGGLELVSWVALDSQNRRTTIRLANQRYGMDVPDTIFRYVDPRQTGR
                     R"
     gene            complement(1957..2124)
                     /gene="rpmG"
                     /locus_tag="VN627_06685"
     CDS             complement(1957..2124)
                     /gene="rpmG"
                     /locus_tag="VN627_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXD02978.1"
                     /translation="MAKPATVKIRLVSTADTGFFYVTKKNPRNSTEKFVFKKYDPVAR
                     KHVEFKEAKIK"
     gene            complement(2283..3059)
                     /gene="ppk2"
                     /locus_tag="VN627_06690"
     CDS             complement(2283..3059)
                     /gene="ppk2"
                     /locus_tag="VN627_06690"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HXD02979.1"
                     /translation="MSKLKRREYADLLKPIQQELEVAARWAEHCGARILVLFEGRDAA
                     GKGGAIHAIAERLNPRQCRVVALGKPTPQEAGQWYFQRYVAHLPAAGEIVLFDRSWYN
                     RAGVEKVMGFATDAQVHQFLEQVPEFERQLVDDGILLFKYWLACDQDEQEERFAERLG
                     DPLKRWKLSPIDLAAREKYEAYTKAREAMLAATHRPDAPWTLVEFNDQKRGRLTLIRD
                     LLDRLPDREVPPDPLELKPLKGKPRKETYRLVEPIEPYKG"
     gene            complement(3164..3448)
                     /locus_tag="VN627_06695"
     CDS             complement(3164..3448)
                     /locus_tag="VN627_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02980.1"
                     /translation="MRTIYKATMMAAVAAMGLSLAACDSKKENAAEDQAQSVRESGDA
                     QADSMEATGEAMGGAAENTLDAKADAVRDAADKKADRMEDKADKMDKAPQ"
     gene            complement(3532..3801)
                     /locus_tag="VN627_06700"
     CDS             complement(3532..3801)
                     /locus_tag="VN627_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXD02981.1"
                     /translation="MGLIILIIVGGILGWLASIVMRTDASQGVFLNVIVGIVGALLAG
                     FILNPLIGGGNIMSGDFSASALLVSFLGAVVLLAIVNLFRRGAMR"
     gene            complement(3946..4758)
                     /locus_tag="VN627_06705"
     CDS             complement(3946..4758)
                     /locus_tag="VN627_06705"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HXD02982.1"
                     /translation="MTLTITAVLLGIVEGLTEFLPVSSTGHLILATELFGFDADTWKV
                     FNVVIQLGAILAVVVQYWRTFWAVGWGILRLEKISLAFLRNLLIAFLPSVVIGLAAKK
                     YIDVLLGSPLVVCAALIVGGFAILYIEKTARTGPYSGIGQLRTGQALGVGLVQCVAMI
                     PGVSRSGATIMGALAMGIERRTAAEFSFFLAVPTMLGATVLELHDNKDALMHGLGPVG
                     WNEIAIGFVVSFVVALAVIKGFVAYISRSGFAPFAWYRIVAGAVAGTLLLMR"
     gene            complement(4916..>5498)
                     /locus_tag="VN627_06710"
     CDS             complement(4916..>5498)
                     /locus_tag="VN627_06710"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HXD02983.1"
                     /translation="AAILQPPFFDPNADAAVNYGAIGGVIGHEMGHGFDDQGAKYDGA
                     GNLNDWWTAADKARFVALTDRLVKQYNGLCPFDNGQTCVNGRLTLGENIGDLGGLSLA
                     YRAYHLSLKGRPAPVIDGLTGDQRFFMAFAQVWRSKYREETARQLLLVDAHSPAQYRV
                     NGIVRNFAEWQKAFGVKPGDKLYLKPEDQIRIW"
BASE COUNT          977 a         1756 c         1814 g          951 t
ORIGIN      
        1 ccaagcggtg gccggtcgat tgcgccgcga gttccggcga ggcccacatg tgatcgagcc
       61 ggcggccccg atccttctcc tgccagaagg tgcgatagga ccaccaggtc cagttccgct
      121 cgggcgccgg gatgtgcttg cggccgagat cgacccagcc gtgggcatcg gcgaagcgct
      181 ggagggtttc gacctccagc ggggtgtggc tgacgacctt gagcagggcc ttgtgatcgt
      241 agacatcaca atccagcggg gcgatgttga aatcgccgac gatcagcgtc ggccgatcca
      301 gctgctctgc ccaacgcgtc atccgcccga ggaaatcgag cttctggccg aactttggat
      361 tggcctcgcg gtcgggaatg tcgccgccgg cgggaatgta gacgttctcc agcaccacgc
      421 cttgcccggg accgagcagt tctacgccga tgtgccgcgc ctcgccattg tcctgccagt
      481 catgccgcga gatctcgcgc agcgggatgc ggctgaccgt ggcgacgccg tgatagccct
      541 tctgcccatg gaccacccgg tgcgtgtagc cggcggcctc gaacacctcg tgcgggaaca
      601 ggtgctcggc gcatttgatt tcctgcaggc acagcacgtc gggctgctcc tgctccagaa
      661 agcggacgac ctgatcggcg cgcaggcgaa ccgaattgat gttccaggtg gcgatagaaa
      721 ccatgcggga ggctttaggc atgcgccgga ggttttgcca gtggcaaggc ggacgcatgg
      781 ccgcagggcg cggcgctgtc ccggtccatg gatgctgaac caggttcagc atgacgagga
      841 tgagaggttg cgctgggtgt ttgggtcaga tcgtcgtcag ccgggagcga gaaatggccc
      901 cgagaccggc gcgcagcgac ctttgatggt cgtgagcacc ggaagccgca ggggacgccg
      961 ttttcgcagc cccggcttgc ggcgatctga gccagacaca agaaaaccct cgtgccgggg
     1021 gcaatggcac gagggttcct tttgcgttcg tcacgctgtg acaaggaagc gatggggagg
     1081 catgggcaac aggggggaaa cccgcctcgc gccgccttgt cgaggaccct tataggcctt
     1141 ccgcattgct cccaagtgaa cggaaactgc aggattgtcg cggtttgtcg tccctatggg
     1201 gcaggtctgc gaccaactgc ggcctctcag cgacgacccg tttgacgcgg atcgacatag
     1261 cggaaaatag tatcgggcac gtccatcccg tagcgctggt tggcgaggcg gatggtcgtc
     1321 ctgcggttct gcgaatcgag cgccacccag ctcaccagct ccagcccgcc aggcgcgccg
     1381 gccttgcggg cgaagatgag ggtgatcacg ccgtattcgg gatgcttgcg gtcgcgcacc
     1441 tcgatcgaca cgatccccgg gtcggtcgcc ggctgcagcg tgccgaagcg gttcacgtcg
     1501 cgcgtggggt cgagcagggc cccgagcggc gaattgccga tgggccagcg ctggacctgg
     1561 cggacctcgt aatcgaccat ggtgagcgcc ttgccatcgc ccacgatgag catgggcacg
     1621 ccctgctggt actggaagcg gatcttgccc ggccgcttga tggtgagctt gccggccacg
     1681 cgctggccgt tgcggtcagt ctgcaggaaa tcggcctgca tcgtggtgat cgcgcgcagc
     1741 gcgtccacgg cctggtcgag atcgttggcg gtctgcgcca cggcaggcgt tgtcagccca
     1801 gccgcgatga ggggagccga cacgacaagc gccgcggaaa ccgcggtgcc cagcgacttg
     1861 cggaaaatgc cgatcaaggt gttcatgcct cgcttgtgaa agagaagcct tgaaccacca
     1921 gtgaacccgc ccggttccgc ggaaccgggc cagcgatcac ttgatcttgg cttccttgaa
     1981 ttcgacgtgc ttgcgcgcga ccggatcgta cttcttgaag acgaacttct cggtcgaatt
     2041 gcgcgggttc ttcttcgtga cgtagaagaa gccggtgtcg gcggtggaga ccagccggat
     2101 cttgacggtt gcgggcttcg ccatggcgac gctttcctaa gcacaggtgt tcaaaacgga
     2161 tcgagcggcg caacagcacc gcccggcaag gttgcggcct gtggggggga atcaccggaa
     2221 agtcaaggaa gagagtcggc gggaggatga gagagcacaa ccctctcccg ggacgaagct
     2281 tctcacccct tgtacggctc gatcggttcg accaaccggt aggtctcctt gcgcggcttg
     2341 cccttcagcg gcttcagctc gagcggatcg ggcggcactt cccgatccgg caagcggtcc
     2401 agcaggtcgc gaatcaaggt cagccggccg cgcttctggt cattgaattc caccagggtc
     2461 cacggcgcgt caggcctgtg ggtggcggcc agcatggcct cgcgggcctt ggtataggct
     2521 tcgtatttct cccgcgcggc cagatcgatc ggcgagagct tccagcgttt gagcgggtcg
     2581 cccagccgct cggcaaagcg ctcctcctgc tcgtcctggt cgcaggcgag ccagtacttg
     2641 aacagcagga tcccgtcgtc caccagctgg cgctcgaact ccggcacctg ctcgagaaac
     2701 tggtgcacct gggcatcggt ggcgaagccc atgactttct cgacacccgc ccggttgtac
     2761 cacgagcggt cgaacaacac gatttcgccc gcggcgggca ggtgggcgac ataacgctgg
     2821 aaataccact gcccggcttc ctgcggggtt ggcttgccaa gggcgacgac ccggcactgg
     2881 cgcgggttga gccgttcggc aatcgcatgg atggctccgc ccttgccggc ggcatcgcgg
     2941 ccctcgaaca ggacgagaat tcgcgcgccg caatgctcgg cccagcgcgc ggccacttcc
     3001 agctcctgct ggatcggctt cagaagatcg gcatattcgc ggcgcttgag cttcgacatg
     3061 ggcgtgcctc cacagaccat cctgcgccat tcggcagtca aacgcaaaac ggccggcggg
     3121 catgcgctcc cgccggccgc gtggcaccgt gtggccgaaa ggcctattgc ggcgccttgt
     3181 ccatcttgtc ggccttgtct tccatgcggt cggccttctt gtcggccgcg tcgcgcaccg
     3241 catcggcctt cgcatcgagc gtgttctcgg ccgcgccgcc catggcctcg ccggtggctt
     3301 ccatcgaatc ggcctgggca tcgcccgatt cgcgcacgga ctgggcctgg tcctcggcag
     3361 cattttcctt cttgctgtcg caagctgcga ggctcagccc catcgccgcg acagcggcca
     3421 tcatcgttgc cttgtagatc gtacgcataa ggtcagcccc ttttgctggt tcaagaaaaa
     3481 agggacggca gcgaaagcta ccgtcccttg tgacgccgtc aggccatacg cttagcgcat
     3541 ggctccacga cggaacaggt tcacgatggc aagaagcacc accgcgccga ggaacgacac
     3601 gagcagcgca ctggcgctga agtcgcccga catgatgttg ccgccgccga tcagcgggtt
     3661 gaggatgaag ccggcgagca gggcgcccac gatgccgact atgacgttga ggaagacacc
     3721 ctggctcgcg tcggtgcgca tcacgatgct ggcgagccac ccgaggatgc cgcccacgat
     3781 aatgagaatg atcagtccca tgacggtaaa ttccttgtgc tgctgtctgc acaaagaaat
     3841 cgtgggaaag cagtcggttc cctgagtctt tgccaggtgc gccctcgaca tccgtcaggg
     3901 gcttgaggga ccggccggag gccctttacg caccggacaa agatctcagc gcatcagaag
     3961 cagcgtcccg gccacggcgc ccgccacgat gcggtaccac gcaaacggcg cgaagcccga
     4021 gcggctgata taggcgacga agcccttgat cacggccagc gccacgacga acgagaccac
     4081 gaagccgatc gcgatctcgt tccagccgac gggtccgagg ccgtgcatca gcgcgtcctt
     4141 gttgtcgtgc agctccagca cggttgcccc gagcatggtc ggcacggcga ggaagaagct
     4201 gaattcggcc gccgtgcggc gttcgatccc catggcgagt gcgcccatga tcgttgcgcc
     4261 cgagcggctg acgccaggga tcatcgccac gcactgaacc agcccaaccc ccagcgcctg
     4321 cccggtgcgc agctggccga tgccgctgta cggcccggtc cttgccgtct tctcgatgta
     4381 gaggatggcg aacccgccaa cgatcagcgc ggcgcagaca accaaggggc tgccgagcag
     4441 cacgtcgatg tacttcttgg cggcaaggcc gatcaccacc gagggcagga aagcgatgag
     4501 caggttgcgc aggaaggcga ggctgatctt ctccagcctg aggatgcccc agcccacggc
     4561 ccagaaagtc cgccagtact gcaccaccac ggcgaggatc gcgccgagct ggatcaccac
     4621 gttgaacacc ttccacgtat cggcatcgaa accgaaaagt tcggtggcga ggatcaggtg
     4681 gccggtggag gataccggga ggaactcggt cagcccctcg acgatgccga ggagcacggc
     4741 ggtgatggtc agcgtcatgg gcgcaggtca tgccggggca gggttgcaga ggcaagacgg
     4801 cgcggcgcgg attgccgatt tgtcatccgg gcaaactctg ccccgcccag ccacggacgt
     4861 ccgtcgtcgc tgcgaagccg ggggcgacgg aggtgcgact ggcggcagac ccccgtcacc
     4921 agatgcggat ctggtcctcg ggcttgaggt aaagcttgtc acccggcttc accccaaatg
     4981 ccttctgcca ctcggcaaaa ttccgcacga tgccgttcac gcggtattgt gccgggctgt
     5041 gggcatccac cagcagcagc tggcgcgccg tttcctcgcg gtacttcgaa cgccagacct
     5101 gggcaaaggc catgaaaaag cgctggtcgc cggtcagccc gtcaatcacc ggcgcgggcc
     5161 ggcccttgag ggagaggtga taggctcggt aggcgaggct caggccgccc agatcgccga
     5221 tgttttcgcc cagggtcagc cgcccgttga cgcaggtctg gccgttgtcg aacgggcaca
     5281 ggccgttgta ctgcttcacc agccgatccg tgagggccac gaagcgcgcc ttgtcggccg
     5341 ccgtccacca gtcgttgaga ttgcccgcgc cgtcatactt ggcgccctgg tcgtcgaagc
     5401 cgtggcccat ttcgtggccg atcacgccgc caatagcgcc gtagttcacg gctgcgtccg
     5461 cattcggatc gaagaagggc ggctgcagga tggcagcc
//
LOCUS       DATNBV010000278         2964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3661060,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000278 DATNBV010000000
VERSION     DATNBV010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3661060"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VN627_06715"
     CDS             complement(<1..415)
                     /locus_tag="VN627_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXD02984.1"
                     /translation="MSYLHFVSNRTTITSAALLSLAVLAGCESTASTESKNPDVANAD
                     PNAGALIGQPPSQLDFSAKLLSVGDRVRFALDRYDLSPEAEATLRQQAALLQNYPQIV
                     VTIEGHADERGTREYNLALGERRADTVRNYLMALGV"
     gene            complement(746..1243)
                     /gene="pal"
                     /locus_tag="VN627_06720"
     CDS             complement(746..1243)
                     /gene="pal"
                     /locus_tag="VN627_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXD02985.1"
                     /translation="MRLKMISAMAAALLLAACASDEAATTGNASAGGGTTDTGITQGA
                     VTPGSAEDFQQNVGDRVFFDTDRYDLSAEAQSQLQKQAAWLAQYPATTVTVEGHCDER
                     GTREYNLALGERRANAVANYLTALGVDAGRLSVISYGKERPECTESNESCWAQNRRGV
                     TAVNK"
     gene            complement(1401..2666)
                     /gene="tolB"
                     /locus_tag="VN627_06725"
     CDS             complement(1401..2666)
                     /gene="tolB"
                     /locus_tag="VN627_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HXD02986.1"
                     /translation="MAAPVKAEPVIDITKGVVEPMPIAVPIFGGANPADNQLGAEIAR
                     VISADLERSGLFAPIDEKAFIQQGLTSDALPRFQDWRLINAQALVAGSVKPGAGGKYS
                     VEFRLWDVFGGAEMFSNSYTFTQDIWRRAAHIVADDIYKRITGEDGYFDTRIVYIAES
                     GPKDNRQKRLAIMDQDGENHQFLTNGADLVLTPRFSPAAQDITYMSYYGNKPRVYLFS
                     ISTGQQEVLGDFPGMTFAPRYSPDGNSVIMSLAQNGNTDIYVMDVRTRRSKRLTDNPA
                     IDTSPSYSPDGTRIVFNSDRGGEQQLYVMNADGSGVIRISKGQGRYATPVWSPRGDLI
                     AFTRIYQGSFYIGVMRPDGSGERMLSQGWLVEGPTWAPNGRVLAFYTQDQSGRTQLHS
                     IDLTGYNERVLKTPMDGSDPAWSPLLSKQ"
BASE COUNT          530 a          939 c          941 g          554 t
ORIGIN      
        1 aaacgccgag ggccatcagg tagttccgca cggtatcggc ccgccgctcg cccagggcca
       61 ggttgtattc ccgcgtcccc cgctcatcgg cgtgcccttc gatcgtgacg acgatttgcg
      121 gatagttctg caggagcgcc gcctgctgcc gcagggtcgc ttcggcctcg ggcgagaggt
      181 cgtagcgatc cagcgcgaaa cgaacgcgat cgccgacact caggagcttg gcgctgaaat
      241 ccagttgcga tggaggctgc ccgatgagcg cgccagcgtt tggatctgcg ttcgcgacgt
      301 caggattctt ggactcggtg ctggccgtgc tctcgcagcc tgccagcacg gcaagcgaca
      361 acagagcagc cgacgtgatc gtcgttcgat tcgacacgaa atgaaggtac gacatgtttt
      421 tccccaagtt gcgttgcaac aatccgcccg gcgcatagga gggatgcaac gcacgccggg
      481 agatacggac ggggatgagg ttcgcttacg cccggggcca tgcaacggcg cggctgaggc
      541 gatgtcacgg tgatttgatg gcgggattga ggcagcgcgc gtggcgcgaa aaaacgggcg
      601 ccgaagcgcc cttcagaaag cactgccccc tccctagcat gttccggtag cggaacgccc
      661 gggagggggc ggaagcggtg tcagatggca aagagccgat tgggaagagc ccgatcctct
      721 tctgctcgct cttctccgcc tctgcttact tgttgaccgc agtcacgccg cggcggttct
      781 gcgcccagca gctttcgttc gactcggtgc actccggacg ctccttgccg taggagatca
      841 cggacagacg gccggcatcg acgcccagcg cggtcaggta attggcgacc gcattggcgc
      901 gacgctcacc gagcgccagg ttgtactcgc gggtgccgcg ctcgtcgcag tggccttcga
      961 cggtcacggt cgtggcggga tattgcgcga gccaggcagc ctgcttctgg agctgcgact
     1021 gcgcttctgc cgacagatca tagcggtcgg tgtcgaagaa cacgcgatcg ccgacattct
     1081 gttggaaatc ctcagcggag cccggcgtaa cggcgccctg ggtaatgccg gtatcagtcg
     1141 taccgccacc agcgctggca ttgcccgtgg tcgcagcctc gtcggaggcg caagctgcca
     1201 gcagcagcgc tgcagccatc gccgagatca tcttaagacg cattttgtcc tctcatcctc
     1261 ttgtttgtct caaaccgtgc gcgcccccca gccccgctac caggaaccaa ggacgccggt
     1321 ttgttcggaa attccgcaac tgatggttgg cattggtgcc gggccctcag aacccccgga
     1381 atcgcgcgga acataaagca ttattgcttg ctgagcaagg gtgaccaggc cggatcggag
     1441 ccgtccatcg gggttttcag gaccctctca ttatagcccg tgaggtcgat ggaatgaagc
     1501 tgtgtacggc cgctttggtc ctgggtgtag aacgccagga cccgcccgtt cggggcccag
     1561 gtcgggccct ccaccagcca gccctgcgac aacatgcgct cccccgaccc gtcggggcgc
     1621 atcactccga tatagaagct cccctgataa atgcgggtga aggcgatgag gtcgccgcgc
     1681 ggcgaccaga ccggggtggc gtagcgccct tggcccttgc tgatgcggat cacgccggat
     1741 ccgtcggcat tcatcacata gagctgctgc tccccgcccc gatcggaatt gaacacgatc
     1801 cgggtgccgt ccggcgaata ggagggcgat gtgtcgatgg ccggattatc ggtcaggcgc
     1861 ttcgaccgac gggtgcggac atccatgacg tagatatccg tattgccgtt ctgcgccaga
     1921 ctcatgatca cgctgttgcc gtccggcgaa taacgcggcg cgaaggtcat gcccgggaaa
     1981 tcgcccagca cttcctgttg gccggtactg atgctgaaaa gatacacccg cggcttattc
     2041 ccgtaatagg acatgtacgt gatgtcctgg gcggcgggcg agaagcgcgg cgtcagcacc
     2101 aggtccgccc cgttggtcag gaactgatgg ttctcgccat cctggtccat gatggccagg
     2161 cgcttctgcc ggttatcctt ggggccggac tcggcgatgt agacgatgcg ggtgtcgaaa
     2221 tagccgtcct cgccggtgat gcgcttgtag atgtcgtcgg ccacgatgtg ggcagcccgg
     2281 cgccagatgt cctgggtgaa ggtgtaggag ttggagaaca tctccgcgcc gccgaacacg
     2341 tcccacaggc ggaactcgac gctgtatttg ccgcccgctc ccggcttcac cgagccggcc
     2401 accaacgcct gggcattgat caggcgccag tcctggaagc gcggcaaagc gtcggaagtc
     2461 agcccctgct ggatgaacgc cttctcatcg atcggcgcga acaggccgga gcgctcgagg
     2521 tcggcggaaa tgacgcgcgc gatctccgcg cccagctgat tgtcggccgg attggccccg
     2581 ccgaatatcg gcaccgcgat cggcatcggc tcgaccacgc ccttggtgat gtcgatcacc
     2641 ggctcggcct tgaccggcgc cgccatgccc agggctgcgc ccaggatcgt ggccgccatc
     2701 gtgaccgccc gcgccgcacc tatcgcgctc cgcaccgtcc aaaccatcgt catgcctcag
     2761 ctcttggatt tcgttctagg aaccttgccg aatcggccga ttggccgaga tttatactga
     2821 tcccctcaag ccttgcctac cggacccgtg gcaggaacga agaaagcctc gtgaggttcc
     2881 tgccgcaaga tcgtggcaat tgtggggctt tggaaactca gcgctactgc accacctgat
     2941 ccgggtggaa gcgtacgacc atcg
//
LOCUS       DATNBV010000279         9052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_179762,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000279 DATNBV010000000
VERSION     DATNBV010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179762"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VN627_06730"
     CDS             <1..288
                     /locus_tag="VN627_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXD02987.1"
                     /translation="HLQAAELLRDPSHGRDYRLGEWRTMLEQSGFRITAITPGRLRMD
                     FAEWTARMRTTPDRVAAIRALQTLASDEVAHHFAIEADGSFTIDTVLIEAS"
     gene            752..1786
                     /locus_tag="VN627_06735"
     CDS             752..1786
                     /locus_tag="VN627_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD02988.1"
                     /translation="MGTTGNESGQEWSEYFKPDWLAMRREEAIEPDLPIIDPHHHIWE
                     WLDYDLKAFLADLTAGHNVRATVHLESGLARDPADPPHLRAVGETRFLMGLRDDSLLK
                     QPGAPDVCAGIVGHVDLDGPEALCEEALVAHLEAGKEHFKGVRFNPYSDPVYSWTPGI
                     PPHLSENPLVRANLKRVAAHGLVCDVMAFHHQLEEIAALARALPNLTIVVNHCGGFLG
                     RAAKHGDYQAAMADWRKGIEAVAREPNTVMKLGGLGSDFFSGNQLHLEPEPPSSEKIA
                     QIYEPFFIPSIEAFGTNRCMFESNYPCDRQQVDYVILWNAFKRLAARLSADEKTDLFS
                     KTASRVYSLG"
     gene            complement(1869..2633)
                     /locus_tag="VN627_06740"
     CDS             complement(1869..2633)
                     /locus_tag="VN627_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD02989.1"
                     /translation="MKTLDFSGKVAIVTGSASGIGLATATQFAQAGALVTLADRDEQA
                     GQAAAESLRMQGARAEFIAVDVADPVQVEALVQRVVSTHGRLDCAFNNAGITQMFGGI
                     EATELATWDKITAIDLSSIFYCLKYQIAAMKALGTGGSIVNNSSDAGLRVVPGIAAYN
                     SAKHGVLALTRTAAREAGANGIRVNAICPGATDTPMMALFTNGEAELVEQFSKNIPLG
                     RFGTPDEIADAVLWLCSDLSRYVTGAVIPVDGGLTA"
     gene            3161..3556
                     /locus_tag="VN627_06745"
     CDS             3161..3556
                     /locus_tag="VN627_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXD02990.1"
                     /translation="MTDYGFSPDVPERAERGIEQYRKLLAGDPKADTPVRGAARDFVF
                     SEVWTRPGLDQRSRFWISLTCACAAGAPIAMTAYMQIARNTGFATIAELREFALQFAV
                     YQGFPRATAVEALIDALEAGEISNDPGQR"
     gene            3668..4849
                     /locus_tag="VN627_06750"
     CDS             3668..4849
                     /locus_tag="VN627_06750"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXD02991.1"
                     /translation="MQQVSAADQANYPSAEALLDAARAETGLSDFGAGNFRIGLGHLL
                     TSLERDARLSPRDRAKALETIRRRLTNRLKIEEYYRLRPETARQEVGGPAIVTGLTRT
                     GSTALGNMLSLDPQFRSLRHWEQAEPVPPPVAGKEADDPRRLAYQAEIDRIYRDDPLR
                     AAQHIHEVDASVEDTEVLGLEFGAQQMTMPVFSYFDWWREADHTATFAYHRRVVQLLQ
                     AGGGSNQWLFKAPHHKFHLDALLSAYPGARFIMTHRDPAKVVPSYTSLVMRGVWDAET
                     AARHDPKVVGPYVSMHLRRGMERAMATRDRIGEDRFIDIHHHDLNADPHGTLERIYAF
                     LVMDLKRSMHDALDEWLKKHRSGAHGEHRYSPEDFGLTAAGIRQDYAFYIDRFNIRTG
                     G"
     gene            4853..5785
                     /locus_tag="VN627_06755"
     CDS             4853..5785
                     /locus_tag="VN627_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD02992.1"
                     /translation="MLSGEKILITGPAGKIAWHVTKKLARDNEVWGIARFSDPAQRDE
                     VEALGVKTRKVDLYECDFGDLPRDFTYLVHIAINFENGSYDRAIRTNAESCGLLLEHC
                     RKAKAALVMSTLSVYKPHPDPWHAFREDDPLGDIMQAIPASYSISKNCEEAVARFCAR
                     AFNLPVTIARMGSAYGPRGGLPINHLDALIAGRLIEPRWDPLPYSPIHGDDIADMIEP
                     LLGAAGVPANIVNFCGDEPVAVQQWIAYMAGLIGVTPQVMVKPQPGASLGSVGDVTKR
                     LSITGPCRIKWQDGLRQVIEELHPGLIKPIGSYA"
     gene            5782..6606
                     /locus_tag="VN627_06760"
     CDS             5782..6606
                     /locus_tag="VN627_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXD02993.1"
                     /translation="MSTIHPRIAVSTISTITWQFPQAVELLRDLGVGYVGILYGMIRD
                     DPQAAVAAMNAAGIACSNVTGDSLGVNLLDPVDADGSPALRGLKPSLDFAASLGKRPC
                     YFTTGPTPPRMPTDEAFDRAVANAAPILAYAKKIGVPLALEHNNAAHRDVGFVNTLHH
                     CVEFSQATGIGICLEIQNCWIERDLPRLFRENMDRITVVQVSDYLVGETTRMNRRVLG
                     DGSIPLEWLLGHLLDAGYKGMFEIETLGPAIEAEGYASAIRRSLDWLNQRLISWGV"
     gene            6652..7872
                     /locus_tag="VN627_06765"
     CDS             6652..7872
                     /locus_tag="VN627_06765"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HXD02994.1"
                     /translation="MTSIPIRTTAPLAPGQLPSWEEQMRLLLPVGQQLIDNWGRSEYS
                     EEDKQDQWVLGLAMLAGGYLGHVYMDPKRPVFVPYWNIALNQGGPNPDYIYNYTAIDP
                     EGTYRISGFRGTNRFTEFSQTHRRVFYMLEEARNSAPITNDLDQLQLGPNGEFSVILS
                     PERPEGHEGDWWRLGEDTQSIIVRRCSTDWRGEVDSRIAIERLDPVETATWSDVSQRW
                     SELPQWIVGMVKFMTDLVKWYRENHPTNGLELSKKITQIGGLPNQFYYDGIYQIDDDE
                     ALVVEVRIPSPCHYWQILVADDRFCTVDWLNRQSSLNDKQARLDADGMLRVVVSKQDP
                     GVPNWLDKEDNKSGILQMRFNRAKEAPPAKVTRVKLADVRQHLPADTPVVTPEERKRI
                     LSARREGAQLRTFW"
     gene            8012..8770
                     /locus_tag="VN627_06770"
     CDS             8012..8770
                     /locus_tag="VN627_06770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD02995.1"
                     /translation="MNMLHDRVAIVTGASSGIGRACARHFAQEGAAVIACARRKPLLD
                     QLADEIRAGGGKALAITCDVSEPAQIKAVVDAAISTFGKIDILANIAQGGLDEQEYLT
                     DLTEERALFMYRTAAVQSLQFMQACLPHMQARGYGRIINTGSHAAVNGEPGFAAYAIA
                     KGAVLTLSRVAAKEWARFGIVTNTILPVVRTDAYDNSAQGREAAERLAATNPTGRLGS
                     PEECAPLLAFLASEGAGYINGQVIGIDGGGMLLA"
     gene            8808..>9052
                     /locus_tag="VN627_06775"
     CDS             8808..>9052
                     /locus_tag="VN627_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD02996.1"
                     /translation="MRLKTPWYHGWNIVGICILMQMTVLGVMVNCFSFFLEGWSRDFH
                     QPISLFVLAISFFAIPAACCMPFAGWVVERWSLSRVM"
BASE COUNT         1722 a         2969 c         2841 g         1520 t
ORIGIN      
        1 cacctgcaag cggccgaact gctgcgcgac ccctcgcacg ggcgcgatta tcgccttggc
       61 gagtggcgaa cgatgctcga acagtcaggc ttcaggatca ccgcgatcac ccccggccgg
      121 ctccgcatgg acttcgccga gtggaccgcg cggatgcgca ccacaccgga ccgggtggcg
      181 gcaatccggg ccttgcaaac gctggccagc gatgaggtcg cacaccattt cgcaatcgaa
      241 gccgatggct cgttcacgat cgacacggtc ctgatcgagg ccagctaggc cgccgcataa
      301 gtcgaactgg acgacttcag catgttccga cctcggccgt aaattcataa atggcaccat
      361 cgaggaagga aagcgcaggg atatgtccat atctcaagct ttcctgccgc cggaatggct
      421 tccgaatccg acgcctcgaa agatgtacat cctccgctcg cccacgagga atggctggtc
      481 ccgattggtg tcgcggaatt tacgcctgaa tacgcggaac aaacagcttg ctggcggcca
      541 cctgctgtta caatagtgta acactaagaa aatgccccgt ccacgagtcg gacatgctgt
      601 ttcgcggcag cgcgcaaggg catccacgac ttccacggcc actacgacaa ctacatgggc
      661 ctcgacgagg atggcgagtt catcgactgt ggtgtcactc ggcgctcatg aacaacaacg
      721 cggaaccaaa acagagagtg gagagcgaaa gatgggaacg acggggaacg agagcgggca
      781 ggaatggagc gagtacttca agcccgactg gctcgccatg cgtcgggaag aagccatcga
      841 gcccgacctg ccgatcatcg atccccatca ccatatctgg gaatggctgg actacgacct
      901 gaaggcattc ctcgccgatc tgacagctgg ccacaatgtg cgggccacag tccacctgga
      961 gagcggtctc gcgcgtgacc cggcggatcc gccgcacctc agggcagtgg gagagacgcg
     1021 gttcctgatg ggactgcgcg atgattcctt gctgaaacag cccggggcgc ccgatgtctg
     1081 cgccggcata gtcggccacg tcgatctgga tggcccggaa gccctgtgcg aagaagcgct
     1141 ggtcgcgcat ctcgaagcag gcaaggagca cttcaagggc gtccgcttca acccctattc
     1201 cgatccggtc tacagctgga cgccaggcat tccgccacac ctgagcgaaa atccgctggt
     1261 cagggcaaac ctcaagcggg tcgctgcgca tggtctggtg tgcgacgtga tggccttcca
     1321 tcaccagctc gaggaaatcg ccgccctggc cagggccctg cccaacctga ccatcgtcgt
     1381 gaaccactgc ggcggctttc tgggccgcgc ggcgaagcat ggcgactacc aggcggccat
     1441 ggcagactgg cgcaagggga tcgaagcggt agctcgcgaa cccaataccg tgatgaagct
     1501 gggcggcctg gggtccgatt tcttctcggg taaccagctg catcttgaac cggagccgcc
     1561 ttcgtccgag aagatcgcgc agatctacga gccgttcttc ataccctcga tcgaagcttt
     1621 cggcacgaac cggtgcatgt tcgaaagcaa ctatccctgc gaccgacagc aggtggacta
     1681 tgtgatcctg tggaacgcgt tcaaacggct cgccgcccgg ctgagcgccg acgaaaagac
     1741 cgacctgttc agcaagacag ccagccgggt ctacagcctg ggatgatccg gaggggcctc
     1801 actgcttgcc ggacgtgagg ccgcgcaaca agtgcgttgc cagcgagcgg cttgcctcgg
     1861 gtcgccggtt aggccgtcag gccgccgtcg accggaatga ccgccccggt aacgtagcgc
     1921 gaaagatcgg agcagagcca tagcacggcg tcggcgattt catccggtgt gccgaacctg
     1981 ccgagcggga tgttcttgct gaactgttcg accagttcgg cctccccatt cgtgaacagc
     2041 gccatcatgg gcgtgtcggt tgcgccgggg cagatcgcgt tgacccttat gccgttggcc
     2101 ccggcctctc gcgccgcggt ccgggtcagg gccaggacgc catgctttgc agagttgtag
     2161 gcggcaattc cgggcaccac ccgcaggccc gcgtccgagg agttgttgac gatcgagccg
     2221 cccgtcccga gcgccttcat cgcggcaatc tgatatttga ggcagtagaa gatactgctc
     2281 agatcgatgg ccgtgatctt gtcccaagtc gccagttcgg tcgcctcgat gccgccgaac
     2341 atctgggtga ttccggcatt gttgaacgcg caatcgaggc gtccgtgcgt gctgacgaca
     2401 cgttgcacga gagcctcgac ctgaaccggg tcggccacgt cgacggcgat gaactcggcc
     2461 cgcgctccct gcatccgcaa cgattcggcg gcggcttgcc cggcctgttc gtcgcggtca
     2521 gccaaggtga ccaaagcgcc cgcttgcgcg aactgcgtgg ccgtagccag gccgatcccg
     2581 gacgcggatc cggtgacgat ggcaaccttg ccggaaaaat cgagcgtctt catgcgcagt
     2641 cactcccagt ttcatgacgg caggcacggg accaggcgca attatgcagt tcctgctttc
     2701 gctgccgcca ccgtgccgcg gtaaggaagg aaagcgttcc tcccgcctta tgtttacacc
     2761 actgtatcct caaccagaca ctccgcaagc gcgctgattg tcgcggcgag ctttggtgtg
     2821 acttgaccga taaccgttga ccccgggctc gctggtcgcc gcagtcagcc cggctgccgg
     2881 cgtccgacgc gcattccaag tccgcacatt tttagtttta catccgtgga tacctaactc
     2941 atagtgcgtc acacaagccc aggcagaaca tgggcggtgg tggagcggct gcaatgaaac
     3001 cgacgcgaat gatcgcgttg cgcgaaccct gtcggccaca gttcgaaaac ttgctgcggc
     3061 gcgtcgaagt cgctgggcag acttattgcg gaacaaggcc gggacaccat gccgccgcaa
     3121 tccgcggcga gcggctgagg gccaagggag tacggtgcaa atgactgatt atggattttc
     3181 cccggacgtt cccgagcggg ccgaacgcgg catcgagcag taccgcaagc tgctggccgg
     3241 cgatcccaag gccgacaccc ccgtgagggg ggccgcgcga gacttcgttt tctccgaggt
     3301 gtggactcgc ccgggacttg atcaacggtc gcgattctgg atttcgctga cttgcgcctg
     3361 tgccgccggg gcccccatcg cgatgacggc ttacatgcag atcgcccgaa acaccggctt
     3421 cgccacgatt gcggaattgc gggaatttgc cctccagttt gccgtctatc aggggttccc
     3481 ccgggccact gccgtcgaag ccctgatcga cgcgctggaa gcgggcgaga tctcgaacga
     3541 tccagggcaa cgatgagggg cttgccgggc cacccccgca aggacaccgt gcgcgaacga
     3601 ccatgatgct gcctccgtgc gacccgctcg cagcaacggc aggaccaccg ggagagacac
     3661 caaagtgatg caacaggtca gcgccgcgga tcaggcgaac tacccatcgg ccgaagcctt
     3721 gctggacgcg gcccgcgcgg agacagggct gagcgatttc ggcgctggca acttccgcat
     3781 cggactgggc cacctgctta ccagcctcga gcgagacgcc cggttgtcgc cgcgcgatcg
     3841 ggccaaggcg ctggagacga tccggcgccg gctgaccaac cgcctgaaaa tcgaagaata
     3901 ctatcgcctc cgtccggaaa cggcccggca ggaggtgggc ggtccggcca tcgtcaccgg
     3961 gctcacgcgt accgggtcga ccgcgctggg caacatgctc tcgctcgatc cccagttccg
     4021 ttccttgcgg cactgggagc aggccgaacc cgtcccgccg ccggttgccg gcaaggaggc
     4081 cgacgacccg cgccgcctcg cctaccaggc cgagatcgac cggatctacc gcgacgatcc
     4141 gctgcgcgcc gcccagcaca tccacgaggt tgacgcctcg gtcgaggata ccgaagtgct
     4201 cgggctcgag ttcggcgccc aacagatgac catgccggtc ttcagctact tcgactggtg
     4261 gcgcgaagcc gatcacaccg cgacctttgc ctaccatcgc cgggtggtcc agctgttgca
     4321 ggcaggcggc ggctcgaacc agtggctgtt caaggccccg caccacaagt ttcacctcga
     4381 cgccctgctt agcgcctatc ccggtgcgcg gttcatcatg actcaccgcg atccggccaa
     4441 ggtcgtgccc agctacacca gcctcgtgat gcgcggcgtg tgggatgccg agacggccgc
     4501 ccgtcacgac ccaaaggtgg tcggccccta cgtttcgatg cacctgcgcc gggggatgga
     4561 acgggccatg gccacgcgtg accggatcgg cgaggaccgc ttcatcgaca tccatcatca
     4621 cgacctcaat gccgatccgc acgggacgct ggaacggatc tatgccttcc tcgtgatgga
     4681 cctgaagcga tcaatgcacg atgcgcttga tgagtggctg aagaagcacc gcagcggcgc
     4741 ccacggcgaa caccgctaca gccccgagga cttcggcctc accgccgccg gcattcgcca
     4801 ggactacgcg ttctacatcg atcgcttcaa catccgcacg ggaggctgag ccatgctgtc
     4861 gggcgaaaaa atcctgatca cggggccggc cggcaagatc gcctggcacg tcacgaagaa
     4921 gctggcccgg gataacgagg tctgggggat cgcccggttc agcgatccgg cccagcggga
     4981 cgaagtcgag gcgctggggg taaagacgcg caaggtcgat ctctacgagt gcgacttcgg
     5041 cgacctgccg cgcgatttca cctacctggt gcacatcgcg atcaacttcg agaacggctc
     5101 ctacgaccgc gcgatccgca ccaacgccga aagctgcggc ctgctgctcg agcattgccg
     5161 caaggccaag gccgcgctcg tgatgagcac gctcagcgtc tacaagccgc atcccgaccc
     5221 gtggcacgcc ttccgcgagg acgatccgct gggcgacatc atgcaggcca ttccggccag
     5281 ctattcgatt tccaagaact gcgaggaagc cgtcgcccgg ttctgcgcgc gggcgttcaa
     5341 cctgccggtg acgatcgccc ggatgggctc ggcctatggc ccgcgcggcg ggctgccgat
     5401 caatcacctg gatgccctga tcgccggccg gctgatcgaa ccacgctggg accccctgcc
     5461 ctacagcccg atccatggcg acgacatcgc cgacatgatc gagccgctgc tcggcgccgc
     5521 cggcgttccg gccaacatcg tcaacttctg cggcgacgag cccgtcgccg tgcagcagtg
     5581 gatcgcctac atggccgggc tgatcggggt gacgccccag gtcatggtca agccccagcc
     5641 cggcgcctcg ctcgggtcgg tgggcgacgt gaccaagcgg ctgtcgatca cggggccgtg
     5701 ccggatcaag tggcaggacg ggctgcgcca ggtcatcgag gaactccacc ccggattgat
     5761 caagccaatt gggagctacg catgagcacc atccatccgc gcatcgcggt gagcacgatc
     5821 agcacgatca cctggcagtt tccccaggcc gtggaactgc tgcgcgacct tggcgtgggt
     5881 tacgtcggca ttctctacgg gatgatccgc gacgatccgc aagccgccgt tgcggcgatg
     5941 aatgccgcgg gcatcgcctg ctcgaacgtg actggcgaca gcctcggcgt gaacctgctc
     6001 gatccggtgg acgccgacgg ttcgcccgcg ctcaggggcc tcaagccctc gctggacttc
     6061 gccgcctcgc tcggcaagcg cccgtgctac ttcaccaccg gcccgacccc gccgcgcatg
     6121 cccaccgacg aggcgttcga ccgggccgtc gccaacgcgg ctccgatcct cgcctatgcg
     6181 aaaaaaatcg gcgtaccgct ggcgctcgag cacaacaatg ccgcccaccg cgatgtcggc
     6241 ttcgtcaaca cgctgcatca ttgcgtcgaa ttctcgcaag ccaccgggat cggcatctgc
     6301 ctggaaatcc agaactgctg gatcgagcgc gaccttccgc ggctgtttcg cgagaacatg
     6361 gaccgcatca ccgtggtcca ggtcagcgac tatctggtcg gcgaaaccac ccggatgaac
     6421 cgccgggtcc tgggcgacgg gtcgatcccg ctcgaatggc tgctcgggca tctgctcgac
     6481 gccgggtaca agggcatgtt cgaaatcgaa acgctgggcc ccgcgatcga ggcggaaggc
     6541 tatgccagcg cgatccgccg cagcctcgac tggctgaacc agcggctgat cagctggggc
     6601 gtgtagacta acttagattg ccatccgtaa ctcccaacga gaggtcctgc gatgaccagc
     6661 attccgatcc gcaccacggc cccgcttgcc cccggtcaat tgcccagctg ggaggagcag
     6721 atgcgcctgc tcctgccggt gggccagcag ttgatcgaca actggggcag gtccgaatat
     6781 tcggaggagg acaagcagga ccagtgggtg ctgggcctcg ccatgctggc gggcggctat
     6841 ctcggccatg tctacatgga tcccaagcgc cccgtcttcg tgccctactg gaacatcgcg
     6901 ctcaaccagg gcgggcccaa tcccgactat atctacaact acaccgcgat cgatccggag
     6961 ggcacctacc gcatctccgg ctttcgcggc accaaccgtt tcaccgagtt ctcgcagacc
     7021 caccggcggg tgttctacat gctggaagag gcccgcaatt ccgcgccgat caccaacgat
     7081 ctcgaccagc tgcaactggg gccgaacggc gagttcagcg tgatcctcag ccccgagcgg
     7141 cccgagggcc atgaggggga ctggtggcgg ctgggcgagg acacgcagtc gatcatcgtg
     7201 cggcgctgct cgaccgactg gcggggtgag gtcgattcac gaatcgcgat cgaacggctc
     7261 gacccggtcg agaccgccac ctggagcgac gtttcgcagc gctggtcgga gttgccgcag
     7321 tggatcgtcg gcatggtcaa gttcatgacc gacctggtga agtggtatcg cgagaaccac
     7381 ccgaccaacg ggctcgaact atcgaagaag atcacccaga tcggcggcct gcccaaccag
     7441 ttctactacg atgggatata tcagatcgac gatgacgagg cgctggtggt ggaggtgcgg
     7501 atcccctccc cctgccacta ctggcaaatc ctcgtcgccg acgaccggtt ctgcacggtc
     7561 gactggctca accgtcaatc gagcctcaac gacaagcagg cccgcctcga tgccgacggc
     7621 atgctgcggg tggtggtctc caagcaggat cccggcgtgc ccaactggct cgacaaggag
     7681 gacaacaagt cgggcatcct gcagatgcgc ttcaaccgcg ccaaggaagc ccccccggcc
     7741 aaggtcaccc gggtcaagct ggccgacgtg cgccagcacc tgccggctga tacccccgtg
     7801 gtcacgccgg aggaacgcaa gcgcatcctc tctgcgcgcc gcgagggcgc ccagctgagg
     7861 accttctggt aagggactgc gcatagagcg caggcggccc gcgatcgccc cctgcgctgg
     7921 gcccgagggc ttgccggagc aatccgggcc ctgggcggag ccggactgac gccttcgccg
     7981 gacgtgccat catacggtgt ggagagatga aatgaatatg ctgcacgacc gggttgcgat
     8041 cgtcacgggc gccagttccg ggatcggccg cgcatgcgcg cgccattttg cgcaggaagg
     8101 cgcagcggtc atcgcctgcg cccggcgcaa accgctactg gatcaactgg cagatgaaat
     8161 tcgcgccgga ggcggaaaag ctctcgccat cacgtgcgat gtctcggagc ccgctcagat
     8221 caaagcagtg gtcgatgccg cgatctcgac cttcggcaag atcgacatcc tcgccaacat
     8281 cgcccagggg ggcctggacg aacaggaata cctcaccgac ctgacggaag aacgggccct
     8341 gttcatgtac cggaccgctg ccgtacagag tttgcagttc atgcaggctt gcctgcccca
     8401 catgcaggcc cgcgggtacg ggcggatcat caacacgggc tcgcacgccg cagtgaacgg
     8461 cgagcccggt tttgccgcct atgcgatcgc caagggcgcg gtgctgaccc ttagcagggt
     8521 ggcagcgaag gaatgggcca gattcgggat cgtgaccaac acgatcctgc cggtcgtccg
     8581 gacggacgcc tacgacaatt cggcccaagg gcgcgaggcg gccgaacgac ttgccgcgac
     8641 taaccccacc ggacgcttgg gctctccgga agaatgtgcg cccctgctcg ctttcctcgc
     8701 gagcgaggga gccgggtata tcaatggaca ggtgatcggg atcgatggcg gcgggatgct
     8761 tctcgcctga ccgcggggag aaacgactga gcaaagggag agcgggtttg cgactgaaga
     8821 caccctggta ccacggctgg aacatcgtgg ggatatgcat cctcatgcag atgaccgtgc
     8881 tgggcgtgat ggtaaactgc ttcagcttct tcctcgaagg ctggtccagg gatttccacc
     8941 agccgatcag cctgttcgtt ctggcgattt ctttcttcgc cattccggcg gcctgttgca
     9001 tgccgtttgc aggctgggtg gtggagcggt ggtccctgtc gcgcgtgatg tt
//
LOCUS       DATNBV010000280         3927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1152977,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000280 DATNBV010000000
VERSION     DATNBV010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152977"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VN627_06780"
     CDS             complement(<1..200)
                     /locus_tag="VN627_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HXD02997.1"
                     /translation="MARKGSARQRGNWRLRLAALVLLVAVIGVVALWWHLRGWTPSRK
                     AFPDQGVEVGTSEGEVDWTALKA"
     gene            complement(246..728)
                     /locus_tag="VN627_06785"
     CDS             complement(246..728)
                     /locus_tag="VN627_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HXD02998.1"
                     /translation="MGQQRISHIELDEATIIARNADIEQERRVALFDLIEDNSFTPVR
                     AFAAGHAGPYRLRLSVQDGRLALEISADDGTLLETLILGLGRFRRHIKDYFAICDSYY
                     QAVRRASATEIETIDMARRGVHNEAAELLLEAFAGKVETDFLTARRLFTLICVLHIRA
                     "
     gene            856..2400
                     /locus_tag="VN627_06790"
     CDS             856..2400
                     /locus_tag="VN627_06790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXD02999.1"
                     /translation="MSEDLLIPALDAAPAPALSGAAVPAARQLPSNIEAEAAFLGAVL
                     IDNRVIEELNTPLKPAHFFEPVHARIYERVTQLLDRKAVVTPVTLKPYFEADEALKAL
                     GGVTYLARLTADGQGLLAPRELAEQIYELALLRELVSVGRNLVENALDTSESVEPLKQ
                     IENAEAALYQVAEGASTNNEAKDFSTATKTALGMIEQALLSGGHVSGKTTGLTSINEK
                     VGGLHDSDLIILAGRPGMGKTSLATNIAFNAADRLRRDRLDGIPDEKSVGAATAFFSL
                     EMSADQLATRILAEQSGISSEALRMGKISRDDFQSLSFASQRLAELPLFIDDTPALTI
                     AGLRARARRLKRRHGLGLVVVDYLQLLQGSGRATDNRVNEISEISRGLKTLAKELSVP
                     VIALSQLSRAVEQREDKRPQLSDLRESGSIEQDADMVWFVYREDYYVAAKEPKRPTES
                     DDAKTHEAHGAWAAEMERVYGLAELIVAKQRHGATGKVRMRFEPRITRFSDLAEDALG
                     GYGPDD"
     gene            complement(2411..2704)
                     /locus_tag="VN627_06795"
     CDS             complement(2411..2704)
                     /locus_tag="VN627_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03000.1"
                     /translation="MSLRKYVAAVNGETFEELEGGLVRATTAEGKSGVFHTDGRWVSG
                     ELRQTNIHMLMFTGGPRIPPEFNYRWPEVPVDTARPSGWPAPLEARLKAIGAL"
     gene            complement(2716..>3927)
                     /locus_tag="VN627_06800"
     CDS             complement(2716..>3927)
                     /locus_tag="VN627_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXD03001.1"
                     /translation="WKKIWQVACREEDIPNVGDYIVYEIAHLSFIVMHSAPGEYKALW
                     NSCPHRARKVADFDGHGVAELKCMYHGWTWQIDGTPKSIPCAWDFPGAGKEFRLVEAK
                     CDIWGGFVLINPDPAAESLASFLGTMPDHYEGAGHDFAKRWKQVHVGAVLPCNWKIAQ
                     EAFLEPWHVPTTHPQFVFERDNDNPSDGRWDDFGNFMRGAPHLPTDRQEAKPGWAVTS
                     EDPQVVIDSHYERHLGMPPQITTHPGTRPTVQIIEATREFYRGIIGDKIDEYHDVDLF
                     GGHMCSLFPNLHPWGAFSHICYRYRPFGTDPDKCIMEVMLLSPWPEDQPRPPAAPIHW
                     LKPGETVADAPELGQLARIFLQDLANVAPTQEGLKTNGLGYVLLAQHNETPVRHFHDL
                     YEKWMGLADGE"
BASE COUNT          663 a         1316 c         1301 g          647 t
ORIGIN      
        1 gccttgagcg cggtccaatc cacctcgcct tcgctggtgc ccacttcgac gccctgatcg
       61 gggaaggcct tgcgcgacgg agtccatccc cgcaggtgcc accacaaggc aacgacgccg
      121 atcacggcga cgaggagcac cagggcggcc agccgcagcc gccagttgcc gcgctgccgc
      181 gctgagcctt tcctcgccat caccggattg ccccgttgtt catgcggcct agccgcggcg
      241 ccccatcagg ccctgatatg gaggacgcag atcaaggtaa agaggcggcg cgcggtgaga
      301 aaatccgtct ccaccttgcc cgcgaaggct tccagcagca gttcggcagc ctcgttgtga
      361 accccgcggc gcgccatgtc gatcgtctcg atctcggtgg cgctggcccg gcgcaccgcc
      421 tggtaatagc tgtcgcagat tgcgaagtag tccttgatgt ggcggcggaa ccggccgagg
      481 ccgaggatca gagtttccag cagggtgccg tcgtcggccg agatttccag cgcgagccgg
      541 ccatcctgga ccgacaggcg cagccggtag ggaccggcat ggcccgccgc gaaggcgcgc
      601 accggcgtga agctgttgtc ctcgatcagg tcgaacaggg cgacgcgccg ttcctgttcg
      661 atatcggcat tgcgggcgat aatcgtcgcc tcgtcgagct cgatatgaga gatgcgctgc
      721 tggcccatgg cgggggcttc ttgcaaatgc gaagggtcgg ggcaagttcc ccgtgcaatc
      781 cacaagctgt ggatagaaag aggcccgccc ccctccttgc ggcaggctgc ggagaggcag
      841 ataaggcggc gctccatgtc cgaagacctc ctcatccccg cgcttgacgc tgctcctgcc
      901 ccagccctgt ccggcgccgc cgtccccgcc gcgcgccagt tgccctccaa tatcgaggcg
      961 gaagccgcat tcctcggcgc ggtgctgatc gacaaccggg tgatcgagga actcaacacg
     1021 ccgctcaagc cggcgcattt cttcgaacct gtccacgccc ggatctatga gcgcgtgacc
     1081 cagctgctcg accgcaaggc agtggtcacc ccggtcacgc tcaagcccta tttcgaggcc
     1141 gacgaggcgc tgaaggcgct gggcggggtg acctaccttg cccgcctcac cgccgacggc
     1201 cagggcctgc tcgccccgcg cgagctggcc gagcagatct acgagctggc cctgctgcgc
     1261 gaactggtca gcgtggggcg caacctcgtc gagaacgcgc tcgacacctc cgagtcggtc
     1321 gagccgctca agcagatcga gaatgccgag gcggcgctct accaggtggc ggaaggcgcc
     1381 tcgaccaaca acgaggccaa ggacttctcc accgcgacca agaccgcgct gggcatgatc
     1441 gagcaggcgc tgctttcggg cggccatgtc tcgggcaaga ccaccggcct cacctcgatc
     1501 aacgagaagg tcggcggctt gcacgattcc gacctcatca tccttgccgg ccgcccgggg
     1561 atgggcaaga cctcgctcgc caccaacatc gccttcaacg cggccgaccg cctgcgccgc
     1621 gaccggctcg acggcatccc ggacgagaaa tccgtaggcg cggccacggc tttcttcagc
     1681 ctcgaaatga gcgccgacca gctggcgacg cgtatcctcg ccgagcaatc gggcatcagt
     1741 tcggaagccc tgcgcatggg caagatcagc cgtgacgact tccagtcgct gtcctttgcc
     1801 agccagcgcc tcgccgaact gccgctgttc atcgacgata ccccggccct caccatcgcc
     1861 ggcctgcgcg cccgcgcgcg gcggctgaag cggcggcatg gcctcggcct ggtggtggtc
     1921 gactatctcc agctgctcca gggctcgggc cgggcgaccg acaaccgcgt taacgagatt
     1981 tcggaaatct cgcgcggttt gaagaccctg gccaaggagc tctcggtccc cgtgatcgcc
     2041 ctttcccagc tcagccgcgc ggtcgagcag cgcgaggaca agcgcccgca gctttcggac
     2101 ctgcgcgaat cgggctcgat cgagcaggac gcggacatgg tctggttcgt ctaccgcgag
     2161 gactactacg tcgccgccaa ggaacccaag cgcccgaccg aaagcgacga tgccaagacc
     2221 cacgaagccc acggcgcctg ggccgcggag atggagcggg tctacggcct cgccgaactg
     2281 atcgtcgcca agcagcgcca cggcgcgacc ggcaaggtcc gcatgcggtt cgaaccgcgg
     2341 atcacgcggt tcagcgacct cgccgaggat gcgctgggcg ggtacgggcc ggacgactga
     2401 gggggggctg tcaaagcgcc ccgatcgcct tgagacgcgc ctccagcgga gcgggccagc
     2461 cgctcgggcg ggcggtgtcg accgggactt ccggccagcg gtagttgaac tccggtggaa
     2521 tgcgcgggcc cccggtgaac atcagcatgt ggatgttggt ttggcgcagc tcgcccgaga
     2581 cccagcggcc gtcggtgtgg aacacgcccg acttgccttc ggccgtggtc gcgcggacga
     2641 ggccgccttc cagttcctcg aacgtctcgc cgttcacggc ggcaacgtat ttgcgcagcg
     2701 acatcgcctg aactcctatt cgccgtcggc cagtcccatc cacttctcgt agagatcgtg
     2761 gaagtggcgg accggggttt cgttgtgctg ggccagcagg acgtagccca ggccattggt
     2821 cttcagcccc tcctgcgtgg gcgccacgtt ggcgaggtcc tgcaggaaaa tccgggcgag
     2881 ctggcccagc tcgggggcgt cagcgaccgt ctcacccggc ttgagccagt gaatcggggc
     2941 cgccggcggg cgcggctggt cttccggcca cgggctgagc agcatgactt ccatgatgca
     3001 cttgtcggga tcggtgccga agggccggta gcggtagcag atgtggctga aggcgcccca
     3061 cggatggagg ttggggaaca ggctgcacat gtgcccgccg aacaggtcga cgtcgtgata
     3121 ttcgtcgatc ttgtcgccga tgatcccgcg gtagaactcg cgcgtggctt cgatgatctg
     3181 cacggtcggc ctagtgcctg gatgcgtggt gatctgcggc ggcatgccga ggtgacgctc
     3241 gtagtgggaa tcgatcacca cctggggatc ttccgaggtg actgcccagc cgggcttggc
     3301 ctcctgccgg tcggtcggca gatgcggcgc gccgcgcatg aagttgccga aatcgtccca
     3361 gcgcccgtcg ctcggattgt cattgtcgcg ctcgaacacg aactgggggt gcgtggtggg
     3421 cacgtgccag ggctcgagaa aggcttcctg cgcgatcttc cagttgcacg ggagcacggc
     3481 gccgacatgg acctgcttcc accgcttggc gaaatcgtgc cccgcgccct cgtagtgatc
     3541 gggcatcgtg ccgaggaagg aagccaggga ttcggccgcc gggtcgggat tgatcaagac
     3601 gaacccgccc cagatatcgc atttcgcctc gaccaggcgg aactccttgc cggcaccagg
     3661 gaaatcccag gcgcagggga tcgatttggg ggtgccgtcg atctgccagg tccagccgtg
     3721 atacatgcac ttgagctcgg caacgccgtg tccgtcgaaa tccgcgacct tgcgggcgcg
     3781 gtggggacag gaattccaca gcgccttgta ttcacccggg gccgaatgca tcacgatgaa
     3841 cgagaggtgg gcgatctcgt acacgatgta gtcgccgacg ttgggaatgt cctcctcgcg
     3901 gcaggcgacc tgccagatct tcttcca
//
LOCUS       DATNBV010000281         3377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2384136,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000281 DATNBV010000000
VERSION     DATNBV010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2384136"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VN627_06805"
     CDS             <1..332
                     /locus_tag="VN627_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXD03002.1"
                     /translation="VAHDGEAFGVRFRKGDMILLALAGSGLDEREFDHALDFDLAREH
                     KAHIAFNSGPHRCVGSHLARIEMRICYEEMLKRLPEFRLDPANPPRMHGGQVIGYDSL
                     PLLWDPA"
     gene            complement(359..886)
                     /gene="msrA"
                     /locus_tag="VN627_06810"
     CDS             complement(359..886)
                     /gene="msrA"
                     /locus_tag="VN627_06810"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXD03003.1"
                     /translation="MAEAIVAGGCFWCTEAVFRDVIGVSEVESGYIGGAGANPTYREV
                     CTGRTGHAEAIRVKFDPDVVSYGELLDIFMATHDPTQLNRQGNDVGTQYRSAIFPLDA
                     DQRAEAEAAIARTQEERAQPVVTTIEGPAPWYPAEDYHQEYWEGEGQRNPYCLAIIPP
                     KLQKLRKSFAARVKA"
     gene            991..1854
                     /gene="aguB"
                     /locus_tag="VN627_06815"
     CDS             991..1854
                     /gene="aguB"
                     /locus_tag="VN627_06815"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HXD03004.1"
                     /translation="MSEAARQITVAALQLPLGSADEGENIAAVAALVEQAAGQGAQIV
                     LPPELFSGPYFCKVEDEARFALARPLAEHPSVRAMQMLAARLKIAIPTSFFERDGHHY
                     YNTLAMIGPDGEIMGSYRKSHIPDGPGYEEKYYFRPGNDGFKVWDVMGTRIGVGICWD
                     QWYPEAARVMALMGAELLFYPTAIGTEPYDAELDTSRMWRRAMLGHAVSNCMPVIAAN
                     RVGTEEGQRFYGHSFISDEWGDFTAEFGAEETGVLVSRINLARAARHRAGMGFFRDRR
                     PQLYTRICEDI"
     gene            1851..2348
                     /gene="folK"
                     /locus_tag="VN627_06820"
     CDS             1851..2348
                     /gene="folK"
                     /locus_tag="VN627_06820"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HXD03005.1"
                     /translation="MSRHSYLVALGSNQRHHRFGSPEQVLQEALARLGGSGLQAGPTA
                     RPMASAPVGPSSRRYANAATIITTTLTPPALLHRLHQIEHEFGRVRRGQPWAARVLDL
                     DIVLWSGGAWSSPGLTVPHVHFRERTFVLRPALAVAPDWRDPLTSLSLRHLHHRLTRP
                     RPVPR"
     gene            2361..2436
                     /locus_tag="VN627_06825"
     tRNA            2361..2436
                     /locus_tag="VN627_06825"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2394..2396,aa:Lys,seq:ttt)
     gene            complement(2681..>3377)
                     /locus_tag="VN627_06830"
     CDS             complement(2681..>3377)
                     /locus_tag="VN627_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXD03006.1"
                     /translation="DIIGKDCGLAAHSWILPNCPPGDPGRQPKSSIYVNARGQRFAAE
                     TAPYAVMPGLIKAQGFKTWGIFDEGARLRSDPTGALISAEWDPQFVLDSVKRGDIIMG
                     DTIEELAGKLGMRPKALRVTIEQWNEDVRAGKDRWFDREMDQIHPIDQGPWYAYRIGL
                     GGFILTGVGPRIDPDCHAYDMDGQIIPGLFAAGEASGGVLGERYVGGGNSVANAITMG
                     RVAGMTIGRELKG"
BASE COUNT          546 a         1221 c         1037 g          573 t
ORIGIN      
        1 tcgttgccca cgacggcgag gccttcggcg tcaggttccg caagggcgac atgatcctgc
       61 tggcgctcgc ggggtcgggc ctcgacgaac gggaattcga ccacgcgctg gattttgacc
      121 tcgcccgcga acacaaggcc cacattgcct tcaattcggg cccgcaccgc tgcgtcggct
      181 cgcacctcgc gcggatcgaa atgcgcatct gctatgagga aatgctgaag cgcctgcccg
      241 aattccggct cgatccggcc aacccgcccc gcatgcacgg cgggcaggtc atcggctacg
      301 acagcctgcc gctgctgtgg gaccccgcct gaggctggat cccgcagcgc ctgcccgctc
      361 aggctttcac ccgggcggcg aagctcttgc gcagtttctg gagcttgggc gggataatgg
      421 ccaggcaata gggattgcgc tggccttcgc cctcccaata ctcctggtga tagtcctcgg
      481 ccggatacca cggggccggg ccctcgatgg tggtgaccac cggctgggcc cgctcctcct
      541 gcgtgcgggc gatcgccgcc tcggcctcgg cgcgctggtc ggcatcgagc gggaagatcg
      601 cggaacgata ctgggtgccg acatcgttgc cctgccgatt gagctgcgtc ggatcgtgag
      661 tcgccatgaa gatgtcgagc agttcgccgt aggaaaccac atcgggatca aacttcacgc
      721 ggatcgcctc ggcatggccc gtgcgcccgg tgcagacctc gcggtaggtc gggttggcac
      781 cggccccgcc gatgtagccg ctttccactt cgctcacccc gatcacatcg cggaacaccg
      841 cctcggtgca ccagaagcac ccgcccgcca caatcgcctc tgccatgcct gcaaactcct
      901 tcatcggtcc ggggcagaga tagggggcgg gcgcggcaat gtcatcgccc cgcggtggac
      961 ttggtccttc gcgcgtccta cttgggggcc atgtccgaag ccgcccgcca gatcaccgtt
     1021 gccgccctcc agctcccgct cggctcggcc gacgagggcg agaacatcgc cgccgtcgcg
     1081 gccctggtcg agcaggccgc cgggcaaggc gcgcagatcg tgctcccgcc cgagctgttc
     1141 tccggcccct atttctgcaa ggtcgaggac gaggcccggt tcgccctcgc ccgcccgctg
     1201 gccgaacatc cctcggtccg ggccatgcag atgcttgccg cgcgcctgaa gatcgccatc
     1261 ccgacctcgt tcttcgagcg cgacgggcac cactactaca acaccctcgc catgatcggc
     1321 ccggacggcg agatcatggg ctcctaccgc aagagccaca tccccgatgg tcccggctat
     1381 gaggagaagt actatttccg ccccgggaat gacggcttca aggtctggga cgtgatgggt
     1441 acgcggatcg gcgtcggcat ctgctgggac cagtggtatc ccgaagcagc gcgcgtcatg
     1501 gccctgatgg gcgccgagct cctgttctat cccaccgcca tcggcacgga accctacgat
     1561 gccgagctcg ataccagccg gatgtggcgg cgggccatgt tgggccacgc cgtgtccaac
     1621 tgcatgccgg tgatcgccgc caatcgcgtc ggcacggaag agggccagcg cttctacggc
     1681 cattccttca tttccgacga atggggcgat ttcaccgccg aattcggagc ggaggaaacc
     1741 ggcgtgctcg tctcccggat caacctcgcc cgcgccgccc gccaccgcgc cggcatgggc
     1801 ttcttccgcg atcgccgtcc ccagctctac actcgcatct gcgaggatat ctgagccggc
     1861 acagctatct cgtcgccctc ggctcgaacc agcgccacca ccgcttcggc tcgcccgaac
     1921 aggtgctgca ggaagccctg gcacggctcg gggggagcgg cctgcaggct ggtccaacag
     1981 cgcggccgat ggcttcggcc ccggtcggcc cgtcgagccg ccgctatgcc aatgccgcca
     2041 ccatcataac gacgacgctc acgccgcccg cgctgctcca tcgcctgcac cagatcgagc
     2101 acgagttcgg ccgcgtccgc cgcggccagc cctgggccgc gcgcgtactc gaccttgata
     2161 tcgtgctgtg gagcggcggc gcctggtctt cgccgggcct caccgtaccc cacgtccact
     2221 tccgcgaacg caccttcgtc ctgcgccccg ccctcgccgt agctcctgac tggcgcgatc
     2281 ccctgaccag cctttcgctc cgccacctcc accaccgctt gacccggcca agaccggtcc
     2341 ctaggtgacg cggcatggtc gggcccttag ctcagtcggt agagcaactg acttttaatc
     2401 agtaggtcgc tggttcgaac ccagcagggc tcaccaccaa acgagttgcc gaaagacacg
     2461 ctgttcctca ccagggacca tgcccgcctc cggctggagg gcggacggat cactgcaacc
     2521 aggaacgatc gcactcgggc ttaggctacg ggcctccggt ggccttcgcc gccgggctgc
     2581 ataagcaaca gcctgcaccg ctacgcccta cgggcttcgc ggcacaggcc attgcttact
     2641 ccgcgctcat gcgcaacaaa acggtgggcc ccggccccgc tcagcctttc agttcacgac
     2701 cgatggtcat cccggcgacc cggcccatgg tgatcgcatt ggcaaccgaa ttgccgccgc
     2761 caacgtagcg ttctccgagc acgccgccac tggcctctcc ggccgcgaag agaccgggga
     2821 tgatctggcc atccatgtca tacgcgtggc aatcgggatc gatgcgcggg ccgacgccgg
     2881 tcagaatgaa cccacccagc ccgatccgat aggcatacca cggcccctgg tcgatgggat
     2941 gtatctggtc catttcccgg tcgaaccagc gatccttccc ggcgcggacg tcctcgttcc
     3001 attgctcgat tgtcaccctg agcgccttgg gtcgcatgcc gagcttcccg gccagttcct
     3061 cgatcgtgtc gcccatgatg atgtcgccgc gcttgacgga atccagcacg aactggggat
     3121 cccattccgc cgagatgagg gcgccggtgg ggtccgaccg caagcgcgcg ccttcatcga
     3181 agatgcccca ggtcttgaag ccctgagcct tgatgagccc aggcatgacc gcatagggcg
     3241 ccgtctcggc agcgaaccgc tggccgcgcg cgttgacgta gatcgaggac ttgggctgcc
     3301 ggccgggatc ccccggaggg cagttgggca agatccacga atgggcggca agaccgcagt
     3361 ccttgccgat gatatcg
//
LOCUS       DATNBV010000282        11264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1752047,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000282 DATNBV010000000
VERSION     DATNBV010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11264
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752047"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /gene="ahpC"
                     /locus_tag="VN627_06835"
     CDS             complement(<1..507)
                     /gene="ahpC"
                     /locus_tag="VN627_06835"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HXD03007.1"
                     /translation="MGIIGSQLGSFSATSFKDGKFVDITEKDVLGKWAVFFFYPADFT
                     FVCPTELEDLADKYAELQAMGVEVFAVSTDTHFSHKAWHDTSPAISKIKYHMVGDQHH
                     TLTNHFNNMREGVGLADRGTFVTDPAGVLQLVEVTPEGVGRNAAELVRKIKAAKYWQE
                     HPGQVCPAK"
     gene            complement(695..1771)
                     /gene="aroC"
                     /locus_tag="VN627_06840"
     CDS             complement(695..1771)
                     /gene="aroC"
                     /locus_tag="VN627_06840"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXD03008.1"
                     /translation="MSFNTFGRLFRFTTWGESHGLALGAVVDGCPPGLPLTEADIQPL
                     LDRRRPGQSKFTTQRQEPDQVRILSGTFEGRTTGTPISLMIENTDQRSKDYGEIAQQY
                     RPGHADYAYDAKYGLRDYRGGGRSSARETAARVAAGAVARLVIPEVSITAWVSEIGGD
                     AIDPDNFDVAEIGNNPFFCPDAAAAVRWEKIVDEARLAGSSIGAVVECEATGVPAGWG
                     APLYAKLDSELAAAMMSINAVKGVEIGDGFGAARLTGEQNADAMRPAGDEHGGPLFLA
                     NHAGGIAGGISTGQPVRVRVAFKPTSSILTPVESINRSGETVQVRTKGRHDPCVGIRG
                     VPVVEAMMALVLADQKLLQRGQCG"
     gene            1869..2219
                     /locus_tag="VN627_06845"
     CDS             1869..2219
                     /locus_tag="VN627_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HXD03009.1"
                     /translation="MTITVYGIPNCDTVKKARTWLEGHNIAHAFHDYKKQGADPARIA
                     GWCAAAGLDKVLNRAGTTFRKLTDGDKAAAGSLDGAVALMTAQPSLIKRPIVEHPGGL
                     LIGFKEAEWAAALG"
     gene            2298..2693
                     /locus_tag="VN627_06850"
     CDS             2298..2693
                     /locus_tag="VN627_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03010.1"
                     /translation="MRRISYPLAALSFGLGALELLSGRKVARRLGLPRSAPVLKTYGL
                     REVASGALLLAAPRSSVGAWSRVAGDVMDLATLGAARLQGRGKRRSMIGAAAFVGAAL
                     AADVAAGLLTLRSVNRRSRWGRSLLAAPA"
     gene            2794..4224
                     /locus_tag="VN627_06855"
     CDS             2794..4224
                     /locus_tag="VN627_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXD03011.1"
                     /translation="MNDQTIHAGKAKVPYAITDADRIPSRRYYDPDFYDLEVRNFWPR
                     TWQMACRLEEIPEQGDYSVYRNVGMSAIVIRTGPDTIKAYDNACRHRGVELVNSRGKA
                     KGGFVCPFHGWRWNAEGKCTHVYAAEAFAKDQLDDADLRLSELKVEVWGGCVFINWDK
                     DAPGYRETLGLAGETLDRFHIEDLRAEWWLSAKVPCNWKVAMEAFMEGYHVATTHTTL
                     RPKGVTNHPDSARWMKMPDDMRFGSRWFTVGPDAPAEVDADVAIEQTIYFMEQLNLGM
                     AGMVAAEEVDVIKAMLGQTELPRDTIAATRALRTKVNEAVTDWYTERGMRVGDFEEID
                     RAGTATYVNFCFPHFFVLPVYGAASSYRIRPLGPEECLFEIWSLKRYPKGEEPPLPPI
                     PEPLAHNDPSWPVVPTEDFRNLPRQQLGLRNPGFEFMRLSDQMEGLISNYHRTLDRFL
                     AGEPRERIAAALLRASGPIESPCQEL"
     gene            complement(4237..4992)
                     /gene="ubiG"
                     /locus_tag="VN627_06860"
                     /pseudo
     CDS             complement(4237..4992)
                     /gene="ubiG"
                     /locus_tag="VN627_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834253.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
     gene            5094..6335
                     /locus_tag="VN627_06865"
     CDS             5094..6335
                     /locus_tag="VN627_06865"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXD03012.1"
                     /translation="MKFGGTSMAGTERIRRVAGIVKRQQEAGHEVAVVVSAMAGETDR
                     LVTFCREANALYDPAEYDVVVAAGEQITSGLLALTLQAMGCKARSWLGWQLPVKTDDA
                     YAKARIEDIDSEALLASMAAGEIAVIPGFQGLSPEGRVTTLGRGGSDTSAVAVAAAVK
                     ADRCDIYTDVDGVYTTDPRIVAKARKLKNVTYEEMLELASVGSKVLQTRSVSLAMKEH
                     VRVQVLSSFIDENAPAADTIPGTMIVSDEELEGLDMERQVITGIAADKNEAKVTLTRV
                     ADRPGAVATIFGPLAEANINVDMIIQNIAKDKGETDVTFTVPAADLLRTQALLEERKE
                     AIGYYRLISDDKVAKISVVGVGMRSHAGVAATMFKALADRGINIQAISTSEIKVSVLI
                     DADEVELAVRVLHTAYGLDAE"
     gene            6491..7597
                     /locus_tag="VN627_06870"
     CDS             6491..7597
                     /locus_tag="VN627_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HXD03013.1"
                     /translation="MPYRTDGTGAEAALKVMLVTSRGTGRWVLPKGNVGARTEPHVAA
                     AIEAEEEAGVLGAVCPVPLGSYRFRKRVRSGASLMADVDVYPLALTRELDSWEEQHER
                     QRCWFTLAEAAKAVDEPDLAELIRSFAPSEFRDAARRTGFLDAVAKTSGVIPMFAWFQ
                     RLLPHSADFFRLFEQHAATVLAAAEATVRVLEGGPAAHEHIREVIEREHDADEITREM
                     LHEVRRTFLTPFDRGAITALIGSLDDTVDEMQATVEAIKLYEVTEFAPQMRDMAAIAL
                     DAARLTAEAMPLLRDVPRNAKRLHELTERLVRMEGHADAVHAEGLKQALKDHGASNTL
                     RFVTEREIYKHLERIVDAFEDVADQIDGIVIDHA"
     gene            7618..8631
                     /locus_tag="VN627_06875"
     CDS             7618..8631
                     /locus_tag="VN627_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HXD03014.1"
                     /translation="MHELAFPLLVALILVALSFDFLNGLHDAANAIATVVATRLLSPV
                     KAVAFAAFFNFSAYFLTLAFPSLHAVADTIGKGLIDKDLVTPGVVFGALVGAMFWNVV
                     TWLKGIPSSSSHALVGGIIGAGVAHAGWSGVQWGGLSKTLIAIVASPLIGFWLALLVM
                     LISSWALHRWTARRAEGTTRVLHLVSSAAYSLSHGLNDAQKTMGVITVLLYSTGYLKG
                     EFHVPHWVAISCYIAMGLGTLSGGWKIIETMGSRITKLSQHQGFSASLAGSIVVFSAT
                     RLGIPVSTTHTITGAIIGAGTARRASAVRWGVAQSVVVAWIITIPASAAVGALFYSLT
                     RLF"
     gene            8809..8970
                     /locus_tag="VN627_06880"
     CDS             8809..8970
                     /locus_tag="VN627_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03015.1"
                     /translation="MTNILVMLAGIAVLVMAPISRERYMRTATQSLAGLVFAVGLVFF
                     VARLFSPVA"
     gene            9292..10272
                     /locus_tag="VN627_06885"
     CDS             9292..10272
                     /locus_tag="VN627_06885"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXD03016.1"
                     /translation="MARGAAFLGTEHAILCGAMSWVSERNLVAAISNAGGFGVIACGA
                     MTPELLDREIAATKELTAKPFGVNLITMHPQLFELIAVCAAHKVGHVVLAGGIPPKGS
                     VEAIKEFGAKVLVFAPTLALAKKLLRAGADALVIEGSEAGGHIGPVATSVLSQEFLPA
                     LGEDYVVFVAGGIGRGEMIAAYLEMGASGVQLGTRFACATESIAHPAFKKAFFRANAR
                     DAVASVQVDPRLPVIPVRALKNNGTEEFTAKQREVAQLLDDGAIEMGEAQLKIEHFWA
                     GALRRAVIDGDVENGSLMAGQSVGMVTKEEPVAEIVAELLTQCEAELSRR"
     gene            10347..10736
                     /locus_tag="VN627_06890"
     CDS             10347..10736
                     /locus_tag="VN627_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXD03017.1"
                     /translation="MSVCGLDHVNIVTADLAGTKRFYKDLLGLTDGDTSALPAGVEAH
                     WLADPTGRSIIHLQRFDPARHGAGRSSGPTGSIDHVALDCTNYDGVRARCEALGVPYR
                     EGLVSPHFSQLFVTDPNDVVLELNFRS"
     gene            10752..>11264
                     /locus_tag="VN627_06895"
     CDS             10752..>11264
                     /locus_tag="VN627_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXD03018.1"
                     /translation="MCDELTPAELAATAVSRREFALAGTVAVLAACSGTHASGAGPSV
                     RESAVSISTPDGMADAAFFHPAKGSHPGIVMWPDIAGIRDANKAMARRLAGAGYAVLL
                     VNQYYRSARAPVLASFAEWRTPEGQERLKPMIAAISPEGTRRDGAAFVAWLDRQAAVD
                     KRRGIGTNGYC"
BASE COUNT         1889 a         3572 c         3814 g         1989 t
ORIGIN      
        1 cttggccggg cagacctggc cggggtgttc ctgccagtac ttggcggcct tgatcttgcg
       61 gaccagctcg gcggcgttgc ggcccacgcc ttccggggtc acttcgacga gctgcagcac
      121 gccggccggg tcggtcacga aggtgccgcg gtcggccagg cccacgcctt cgcgcatgtt
      181 gttgaagtgg ttggtcagcg tgtgatgctg gtcgcccacc atgtgatact tgatcttgct
      241 gatcgccggc gacgtgtcat gccaggcctt gtggctgaag tgggtgtcgg tcgagaccgc
      301 gaagacttcc acgcccatcg cctggagctc ggcatacttg tcggcgaggt cttcgagctc
      361 ggtcgggcag acgaaggtga agtcggccgg gtagaagaag aacaccgccc acttgccgag
      421 aacgtccttc tcggtgatgt cgacgaactt gccgtccttg aacgaggtgg ccgagaacga
      481 gccgagctgg ctgccaatga tacccatgga tctactctcc tgttgcggtg aatttcagcg
      541 acaggggagg ggataggccc ggctgggcga gtcgggaaat gcggattccc gattaatcag
      601 atttgtcctg ccgattaccg taaactaatc ggcgaaggcg atcagtcgtg ttggatttcg
      661 catgagggcg cctgcacgta ttttctcaac aaacctaccc gcactgcccc cgctgcagca
      721 gcttctgatc cgccagaacc agcgccatca tcgcctccac caccggcacc ccgcgaatgc
      781 cgacgcaggg atcatgccgc cccttggtcc gcacctgcac cgtctcgccg ctgcggttga
      841 ttgattcgac cggcgtgagg atcgaactcg tcggcttgaa cgccacccgc acccgcaccg
      901 gctgcccggt cgagattccg cccgctatgc cgcccgcgtg gttggccagg aacagcggcc
      961 ccccgtgctc gtcgccggcg gggcgcatgg catcggcatt ctgctcgccg gtcaaccggg
     1021 ccgccccgaa gccgtcgccg atctccacgc ccttgaccgc attgatgctc atcatcgccg
     1081 cggccagctc gctgtcgagc ttggcataga gcggcgcgcc ccagccggcg ggcacgccgg
     1141 tggcctcgca ttccaccacc gcgccgatgg acgagcccgc cagccgcgcc tcgtcgacga
     1201 tcttctccca ccgcacggca gccgccgcat ccgggcagaa gaacgggttg ttgccgatct
     1261 cggcgacatc gaagttatcg gggtcgatcg catccccgcc gatctcgctg acccaggccg
     1321 tgatcgacac ttccggaatc accagccgcg ccaccgcgcc agccgccacc cgcgccgccg
     1381 tctcccgcgc cgatgaccgc ccgccgccgc gatagtcgcg caggccgtat ttggcgtcat
     1441 aagcatagtc ggcgtggccg gggcgatatt gctgggcgat ctcgccatag tccttggagc
     1501 gctggtcggt attctcgatc atcaggctga tcggcgtacc ggtggtgcgg ccctcgaacg
     1561 tgcccgagag gatgcggacc tggtccggct cctggcgctg ggtggtgaac ttcgactggc
     1621 ccgggcggcg gcggtcgagc agcggctgga tgtccgcctc ggtcagcggc aggcccggcg
     1681 ggcagccgtc gaccacggcg cccagggcga gcccgtggct ctcgccccag gtggtgaaac
     1741 ggaacaggcg gccgaaggtg ttgaacgaca tggggcgcgc ttttgcgcaa gaagcgcgca
     1801 aaagtccacc ggcggccttg cccaagccct gcggcgcgct actcaaaccg caaaagagga
     1861 ccctcgcgat gacgatcacg gtttacggca ttcccaactg cgacacagtc aagaaggcgc
     1921 gcacctggct cgaaggccat aacattgccc atgcgttcca tgactacaag aagcaggggg
     1981 cagacccggc gaggatcgcc ggctggtgcg ctgccgccgg gctggacaag gtgctgaacc
     2041 gggcgggcac gaccttccgc aagctgacgg acggcgacaa ggctgcggcc ggctcgcttg
     2101 acggcgcagt cgcgctgatg accgcgcagc cgtcgctgat caagcggccc atcgtcgagc
     2161 atccgggagg cctgctgatc ggcttcaagg aggcagagtg ggccgccgcg ctcggctgac
     2221 cagttgcggg gaccggctgc ggaacggcag gtcacgcggc gcgcttctgg aagggagacc
     2281 atcaggagag gcccaccatg cgtcgtatct cttacccgct cgccgccctc agctttggac
     2341 tgggcgcgct ggaactgttg tccggacgca aggtcgcccg caggctgggc ctgccgcgtt
     2401 cggcgccggt gctgaaaacc tacgggctgc gggaagtggc cagcggcgcc ctgctccttg
     2461 ccgcaccgcg gtcgagcgtg ggagcatgga gccgggtagc gggggacgtc atggacctgg
     2521 cgacgcttgg cgcggcgcgc ttgcagggac gggggaagcg tcgctcgatg attggcgctg
     2581 ctgcctttgt gggcgcggcg ctggcggctg acgtggctgc cggattgctt acgctgaggt
     2641 ctgtcaaccg ccggagccga tggggacgat cgctccttgc ggcacctgcg tgatcgcagc
     2701 gcggatcatg tcggtgatct tgctgcccgg gaggccttgt tgggccgatc tgatcgatta
     2761 agctgccctg caagcgattg gcgggagcag cgaatgaacg accagacgat ccatgcgggc
     2821 aaggccaagg tgccctatgc gataacagat gccgatcgaa ttccctcccg ccgttattac
     2881 gacccggact tctatgatct ggaggtccgc aacttctggc cgcgtacctg gcagatggct
     2941 tgcaggctcg aggaaattcc cgaacaggga gattattcgg tctatcgcaa cgtgggcatg
     3001 tcggcgatcg tgatccgcac tggcccggac acgatcaagg cgtacgacaa tgcctgccgc
     3061 caccgcggcg tcgaactggt caacagccga ggcaaggcca agggcgggtt cgtttgcccc
     3121 ttccatggct ggcgctggaa tgccgagggc aagtgcaccc atgtctatgc agccgaggcg
     3181 ttcgccaagg accagctcga tgatgccgac ctcaggctgt cagagctgaa agtcgaagtc
     3241 tggggcgggt gtgtgttcat aaattgggac aaggacgccc ccggatatcg ggagacgctg
     3301 ggtctcgccg gggaaacgct ggatcggttc cacatcgaag atctgcgcgc cgaatggtgg
     3361 ctttccgcca aggtgccgtg caattggaag gtcgcgatgg aagccttcat ggaaggctac
     3421 catgtcgcca ccacccacac gaccctgcgt cccaagggtg tgacgaacca tcccgacagc
     3481 gcccgctgga tgaaaatgcc ggacgacatg cgttttggca gtcgctggtt caccgtggga
     3541 cccgatgcgc ccgccgaagt cgacgccgac gttgctattg aacagacgat ctatttcatg
     3601 gagcagctga acctcggcat ggcgggaatg gtcgccgccg aggaggtcga cgtcatcaag
     3661 gcaatgcttg gccagacgga gcttccgcgc gacaccattg ccgcaaccag ggcgttacgg
     3721 accaaggtga atgaggcggt caccgactgg tacacagagc gcggcatgag agttggcgat
     3781 ttcgaagaga tcgaccgggc gggcaccgcg acctacgtga acttttgttt cccgcacttc
     3841 ttcgtacttc cggtatatgg cgcagcgtcg agctaccgga tcaggccgct ggggccggag
     3901 gagtgtctct tcgaaatctg gtcgttgaag cgctatccga aaggcgagga gccgccgctg
     3961 ccgccaattc cagagcctct ggcccacaat gatccaagct ggccggtggt gcccaccgag
     4021 gatttccgca accttccgcg ccagcagctg ggattgcgca acccgggctt cgaattcatg
     4081 cgcctgtcgg accagatgga ggggctgatc agcaactacc accggacgct ggaccgtttc
     4141 ctggccggtg aaccgcgcga gcggattgcc gcggccctgc ttcgggccag cgggccgatc
     4201 gagtcgccct gccaggagct gtgaccggac tattggtcag gcgcaggccg tggccgttac
     4261 gatgtagttc agcgaaaggt cggtcgccag gtggagcccg gcgagggggc tccatgcgat
     4321 gccctgcggc tccgccatgg cgaggccggc ggcggacagc agctcgcgca gctcgtccgg
     4381 agtgatgaaa tcgtcccagt gatgggttcc tcgcggtatt ccgccgaagc gctcggctgc
     4441 cccgacgagg atcaggcgcg agcggagggt acggttgggg gttgaaagga tcatcaggcc
     4501 tcccggcgcc agactggcgc cgagtgcccg cacgaaagcg cccttgtcgg ccacgtgctc
     4561 gatcacttcc atcgaggtga cgagatcgaa ctggccgaga cccaggctcg cgagatcgcc
     4621 atgccggtaa tcgatggata gacctgaacc ttgggcatgg gcagtggcgg ccgcgacgtt
     4681 ttcagccgcc gcgtccacgc cgctgaccga cgcaccaagc cgggcgagcg gctccgtcaa
     4741 cagtcctgcg ccgcagccca cgtcgagcgc gcgcttaccg gcaagtggct tgagagatcg
     4801 cgaatcgccg tgccagtgcc gatccaccgc atcgcggata aacgccatgc gcactgggtt
     4861 gagccggtgg agcatggccg aggagccctt cggatcccac cactcggccg ccatgcgccc
     4921 gaaatgggcc gcttcctagg ggcggatcgt cgggtcggag gaatgggggg tgttggaggt
     4981 tgcattagcc atggaccctt cctaaagcgc gcccgccctt cggtccatgc agcccgcggg
     5041 gaaagatttg gtggaccccg cagcaggagc acaggccagt ggctcgcatc gtgatgaaat
     5101 tcggcggcac gtcgatggcc gggaccgagc gcatccggcg cgtggcgggc atcgtcaagc
     5161 gccagcagga ggccgggcat gaggtcgccg tggtggtctc ggccatggcc ggggaaaccg
     5221 accggctggt gaccttctgc cgggaggcca atgcgctcta cgacccggcg gaatatgacg
     5281 tcgttgtcgc tgcgggcgag cagatcactt cggggctgct ggccctgact ctccaggcca
     5341 tgggctgcaa ggcgcgctcg tggctgggct ggcaactgcc ggtgaagacc gacgacgcct
     5401 atgccaaggc gcggatcgag gatatcgaca gcgaggccct gctggcctcc atggcggcgg
     5461 gcgaaatcgc ggtgattccg ggctttcagg gcttgtcgcc ggaaggccgc gtgaccacgc
     5521 tgggccgcgg cggttcggat acgtcggccg tggccgtggc cgctgccgtg aaggccgacc
     5581 ggtgcgacat ctacaccgac gttgacggcg tctacaccac cgatccgcgg atcgtggcca
     5641 aggcacgcaa gctcaagaac gtgacctacg aggaaatgct ggagctggcc tcggtcggtt
     5701 ccaaggtgct ccagacccgg tcggtttcgc tggcgatgaa ggagcatgtg cgggtgcagg
     5761 tgctatcttc gttcatcgac gaaaacgccc ctgccgctga taccattccg gggacaatga
     5821 tcgtttccga cgaagaactt gaaggactgg acatggaacg ccaggtgatc accggcatcg
     5881 ctgccgacaa gaacgaggcc aaggttaccc tgacccgcgt ggccgaccgc cccggcgccg
     5941 tggcgacgat cttcggcccg ctggccgagg ccaacatcaa cgtcgacatg atcatccaga
     6001 acatcgccaa ggacaagggc gagaccgacg tcacctttac cgtgccggct gcagatctgc
     6061 tgcgcaccca ggctctgctg gaagagcgca aggaggccat cggctactac cgcctgatca
     6121 gcgacgacaa ggtcgccaag atctcggtcg tgggcgtggg catgcgcagc cacgcgggtg
     6181 ttgctgccac gatgttcaag gcgctggcgg atcgcgggat caacattcag gcgatctcca
     6241 ccagtgagat caaggtttcg gtcctgatcg acgccgacga ggtggaactg gccgtgcgtg
     6301 tgctgcatac tgcatacgga ctcgacgccg agtaaactgc aacagccctg caaccggggt
     6361 gaaagccggg cacaacccgg ctccagcggt tgcatggcca gcggaatttg tgacacggat
     6421 atgtcacggc gcggcgaggg gccgctccgg ctgacgttcg gggaacaggg tcattcgcca
     6481 ggtcgctgcc ttgccatacc gcaccgacgg gacgggcgcc gaggctgccc tcaaggtgat
     6541 gctcgttacc tcgcgcggga cgggtcgctg ggtcctgccc aagggcaatg tcggggcccg
     6601 caccgagccg catgtcgccg ccgcgatcga ggcggaggag gaggccgggg tgctcggcgc
     6661 ggtttgcccg gtgccgctgg gctcctaccg gttccgcaag cgggtgcgtt cgggcgcttc
     6721 gctgatggcc gacgtcgacg tgtacccgct ggcgctgacc cgcgagctcg attcatggga
     6781 agagcagcat gagcgccagc gctgctggtt taccctggcc gaggcggcca aggcggtgga
     6841 cgagcccgac cttgccgaac tgatccgttc cttcgcacct tccgaatttc gtgatgctgc
     6901 ccggcgcacc ggcttcctcg atgccgtcgc caagacttca ggggtaatac cgatgtttgc
     6961 ctggtttcag cgcctgcttc cgcacagcgc agacttcttc cggctgtttg agcagcacgc
     7021 cgccaccgtg ctcgccgccg ccgaggcaac cgtgcgggtg ctcgagggcg ggccggccgc
     7081 ccatgagcac atccgcgagg ttatcgagcg cgagcacgac gccgacgaga tcacgcgcga
     7141 gatgctgcac gaggtgcgcc gcaccttcct tactccgttc gaccgcggtg cgatcaccgc
     7201 cctgatcggc tcgctcgacg acacggtgga cgagatgcag gccacggtcg aagcgatcaa
     7261 gctctacgag gtgaccgagt tcgccccgca gatgcgcgac atggcggcga tcgcgctgga
     7321 cgcggcgcgg ctgaccgccg aagccatgcc gctcctgcgc gacgttccgc gcaacgccaa
     7381 gcggctgcac gagcttaccg agcggttggt gcgcatggag ggccatgccg atgcggtgca
     7441 tgccgagggc ctgaaacagg cgctcaagga tcacggcgcg tccaacacgc tgcgcttcgt
     7501 caccgagcgc gagatctaca agcatctcga gcggatcgtc gatgccttcg aggatgttgc
     7561 cgaccagatc gacggcatcg tgatcgacca cgcctgagcg gctgaggggg cactgccatg
     7621 cacgagctgg cctttccgct gctggtggcg ctgattcttg tcgcgctttc cttcgacttc
     7681 ctcaacggac tgcacgatgc ggccaacgcc atcgccaccg tggtcgcgac ccggctgctt
     7741 tcgccggtca aggcggtcgc cttcgccgcg ttcttcaact tctccgccta tttcctgacg
     7801 ctggctttcc cgagcctgca tgcggtggcc gataccatcg gcaagggcct gatcgacaag
     7861 gatctggtga cgccgggagt ggtgttcggg gcgctggtgg gggcgatgtt ctggaacgtc
     7921 gtcacctggc tcaagggcat tccctccagt tccagccatg ccttggttgg cgggatcatc
     7981 ggtgcgggcg tggcccatgc cgggtggtca ggcgtgcagt ggggcggcct ctccaagacc
     8041 ctgatcgcca tcgtcgcctc gccgttgatc gggttctggc tggcgctgct ggtaatgctg
     8101 atcagttcct gggcgctgca tcgatggact gcgcggcggg ccgaggggac gacgcgggtc
     8161 ctgcacctgg tatccagcgc ggcctattcg ctgagccatg gcctcaacga cgcgcagaag
     8221 acgatgggcg taatcaccgt gttgctttac tcgaccggct acctgaaggg cgagttccac
     8281 gtgccgcact gggtggcgat ctcgtgctat atcgccatgg gcctgggtac gctgtcgggc
     8341 gggtggaaga tcatcgagac catgggctcg cggatcacca aactttcgca gcatcaggga
     8401 ttttccgcgt cgctggcagg ctcgatcgtg gtgttctcgg cgacacgcct cggcattccg
     8461 gtgtcgacca cgcacaccat caccggcgcg atcatcggcg ccggaacggc ccggcgggcc
     8521 tcggccgtgc gctggggcgt ggcgcagagc gttgtcgttg cctggatcat aaccattccc
     8581 gcctcggcgg ccgtgggagc gctgttctat tcgctgacgc ggctgttctg aggcgccttg
     8641 agggacgtca gggggagggg tcgaccgctg ttgtgcgccc gtcgcgcgac gtagtgccca
     8701 ataaaaccct aggggtgatg gttaagatct gttggggaca actgtttgac ggaaggtaac
     8761 cagccctgtg cgacatggcg ggtcccaccg gaaagagtac caattatgat gaccaatatt
     8821 ctcgtcatgc tggcaggcat cgctgtcctc gtgatggccc ccatctcccg cgaacgctac
     8881 atgcgcaccg cgacccagag ccttgcgggg cttgtctttg cggtcggtct ggtatttttc
     8941 gtggctcggt tgttttcgcc ggtcgcctga cgcccacgat tgagccccgc cgcgccaaag
     9001 ggcggcgacg gggctcttgc gacccgatcg acccagccaa agggggaacg gcgggcggcg
     9061 ggctcctcag ggtgaaccga tccgtgtcgg cctcttcgga tgcaaacctt ttaaccacca
     9121 ttcgctgttc gcggaattga gattggctgg ctagtgtgat cgttcctgcc gatttcagcg
     9181 gcgggggttt atccgcggca atccagcttc tatagcgcct ccgccgcgcg gcacctgtcg
     9241 gcgggtccgg gaacgatcgg gggttacgtg tacgacaaga ccaaggcgct catggcgcgc
     9301 ggggccgcat tcctgggcac cgagcatgca atcctgtgtg gtgcgatgag ctgggtgtcc
     9361 gaacgcaacc tggtggccgc gatcagcaac gcgggcgggt tcggcgtgat tgcctgcggg
     9421 gcgatgaccc ccgagttgct tgaccgtgag atcgcggcga cgaaggaact taccgccaaa
     9481 ccgttcggcg tgaacctgat caccatgcac ccccagctgt ttgagctgat cgcggtctgc
     9541 gccgcgcaca aggtcggcca tgtcgtgctg gccggcggca ttccgcccaa gggttcggtc
     9601 gaagcgatca aggaattcgg cgcgaaagtg ctcgttttcg cgccgaccct cgcgctggcg
     9661 aagaagctgc tgcgcgccgg cgccgatgcc ctggtgatcg agggttcgga agcgggcggt
     9721 catattggcc cggttgcgac gagcgtgctg tcgcaggaat tcctcccggc attgggggaa
     9781 gattacgtgg tcttcgtggc gggggggatc ggccgtggcg agatgatcgc ggcctacctc
     9841 gaaatgggcg cttcgggcgt ccagctgggt acccgctttg cctgcgcgac cgaatcgatc
     9901 gcgcaccctg cgttcaagaa ggccttcttc cgcgccaatg cgcgtgacgc cgtggccagc
     9961 gtgcaggtcg atccgcgcct gcccgtgatc cctgtccgcg cactcaagaa caacggaacc
    10021 gaggaattca ccgccaagca gcgcgaggtg gcgcaactgc tcgacgatgg ggccatcgaa
    10081 atgggcgagg cccagctcaa gatcgagcac ttctgggccg gcgccctgcg ccgcgccgtg
    10141 atcgatggcg atgtcgagaa cggttcgctg atggccggtc agtcggtggg catggtgacg
    10201 aaggaagagc ctgttgccga gatcgtcgcc gaactgctca cccagtgcga ggcagagctc
    10261 tcccgccgct gaggccaggg tcagaaccct agacccggcg gcgcgcctct ggcaggcttg
    10321 tgccttgcgg tagccggaga gagctgatgt cggtttgcgg gcttgatcac gtcaacatcg
    10381 ttacggccga ccttgccggc accaagcggt tctacaagga cctgctgggt ctgacggacg
    10441 gcgatacctc ggctcttccc gccggagtcg aagcccattg gctggccgac cctacaggcc
    10501 gctcgatcat ccacctgcaa cggttcgatc cggcgcggca tggcgccggc cgctcgtcgg
    10561 ggccgaccgg ttcgatcgac catgttgccc ttgactgcac gaattacgac ggcgttcgcg
    10621 cgcgctgcga agcgctgggc gtgccgtacc gggaggggct cgtctccccc cacttctccc
    10681 agctgttcgt gaccgatccc aacgatgtcg tgctcgagct gaactttcgc agctgattga
    10741 aaggcttcgc catgtgcgat gaacttacgc ccgccgaact ggctgctacg gcagtctcgc
    10801 ggcgcgagtt tgcgctggcc gggacagtgg cggtgctggc ggcctgtagc ggcacgcacg
    10861 cgtcgggcgc cggcccgtcc gtccgcgaat ctgccgtttc catctcgacg ccggacggca
    10921 tggccgatgc tgcgttcttc catcccgcca agggcagcca tccggggatc gtgatgtggc
    10981 ccgacattgc cggtatccgc gatgccaaca aggccatggc gcggcggctg gcaggggccg
    11041 gttatgcggt gctgctggtc aatcagtact atcgcagcgc gagggcgccg gtgctcgcca
    11101 gctttgccga atggcgcacc ccggaaggac aggagcggct caagccgatg atcgcggcga
    11161 tcagccccga gggcacgcgg cgcgacggcg cggcattcgt tgcctggctg gaccggcagg
    11221 ccgccgtcga caagcggcgc gggatcggca ccaacggcta ttgc
//
LOCUS       DATNBV010000283         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2726010,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000283 DATNBV010000000
VERSION     DATNBV010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2726010"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /gene="priA"
                     /locus_tag="VN627_06900"
     CDS             <1..1231
                     /gene="priA"
                     /locus_tag="VN627_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXD03019.1"
                     /translation="EAHEVSFKQDDGVRYNARDVAVIRAKFEGIPVILASATPALESM
                     QLAEAGVYRRIELAARYGGAELPAIRIVDLREAQPERQHWLSPPLVKELKDRLLRGEQ
                     SLLFLNRRGYAPLTLCRNCGYRFQCPNCTAWLVEHRLSRRLACHHCGHEVPTPEACPE
                     CGTPDCMVACGPGVERIADEVAERFPEARVAVVTSDTLNTPGKVGEFVENVENKLIDV
                     IVGTQLVTKGYHFPELTLVGVIDADLGLEGGDLRAGERTYQQIAQVAGRAGRGEKPGE
                     VLIQTRHPTAPVIAALASGDRDAFYDAEAESRREAGAPPFGRWAAIIVSSEDEAEARD
                     AARALGGTAPRLPDLMVLGPAPAPMALLRGRYRYRLLVNARRSVQLQAIIRDWLGALS
                     FPSGVRVAVDVDPYSFV"
     gene            1247..1921
                     /locus_tag="VN627_06905"
     CDS             1247..1921
                     /locus_tag="VN627_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HXD03020.1"
                     /translation="MIARELLGTARVPGGEELRLFRHDRDFMIVLGANELMSSRMSGS
                     EVALATETLARLGKPNAPCMLIGGYGMGFTLRAAIANTGPKAELIVCELVPEIIEWAR
                     GPMTDLAAGCLEDPRVRLMMGDVAFAIDGARSEYDAILLDVDNGPDGLVRPDNNGLYG
                     KSGLRAAMNALKPGGIVAYWSAYADPAFTRRLERTGFDVEEITVRARSNGKGPRHTIW
                     FGRRPN"
BASE COUNT          338 a          697 c          708 g          351 t
ORIGIN      
        1 cgaagcgcat gaagtctcgt tcaagcagga cgatggggtg cgctacaacg cgcgcgacgt
       61 ggcggtcatc cgggccaagt tcgaaggcat tccggttatc ctcgccagcg ccacgcccgc
      121 cctcgaatcg atgcaactgg ccgaggccgg agtttaccgg cggatcgagc tggccgcgcg
      181 ctacggcggg gcagaactgc ctgcgatccg gatcgtcgac ctgcgcgagg cccagcccga
      241 gcgccagcac tggctgtccc cgccgctggt caaggagctg aaggaccggc tgctgcgcgg
      301 ggagcaatcg ctgctgttcc tgaaccgccg cggctatgcg ccgctgacgc tgtgccgcaa
      361 ttgcggctac cgcttccagt gcccgaactg caccgcctgg ctggtcgagc accgcctttc
      421 gcgccgactt gcctgccacc attgcggcca cgaggtgcct acgcccgagg cctgccccga
      481 atgcggcacg cccgattgca tggtcgcctg cgggccgggg gtagagcgga tcgccgacga
      541 agtggccgaa cgcttcccgg aagcccgggt ggccgtggtc acctccgaca cgctcaatac
      601 ccccggcaag gtcggcgaat ttgtcgagaa tgtggagaac aagctgatcg acgtgatcgt
      661 cggcacccag ctggtcacca agggctacca cttccccgaa ctgaccctcg tcggcgtgat
      721 cgacgccgat ctaggccttg aagggggcga cctgcgcgcg ggcgagcgga cctatcagca
      781 gatcgcgcag gtggccgggc gtgcgggacg cggggagaag cccggtgagg tcctgatcca
      841 gacccgtcat cccaccgcgc ccgtcattgc tgcccttgcc agcggcgacc gcgacgcgtt
      901 ctacgacgcc gaggccgaaa gccgccgcga ggcgggcgcc ccgccgttcg ggcgctgggc
      961 tgcgatcatc gtctcctcgg aggacgaggc cgaggcccgc gacgccgccc gcgccctagg
     1021 cggcacggcc ccgcgcttgc ctgacctgat ggtgctcggc cccgctcccg ccccgatggc
     1081 cctgctgcgc ggtcggtacc gctatcgcct gctggtgaac gcgcgccgct ctgtgcaatt
     1141 gcaggccatt attcgcgatt ggctcggcgc gctcagcttt ccctccgggg tgcgggtggc
     1201 cgttgacgtc gatccctaca gctttgtctg acgaaggaat tcaatgatga tcgctcgcga
     1261 actgctcggc acggcccggg tccccggcgg cgaggaattg cggctgttcc ggcatgaccg
     1321 cgatttcatg atcgtgctcg gcgcgaacga actgatgagc agccggatga gcggctcgga
     1381 agtagcgctg gccacggaaa cccttgctcg gctcggcaag ccgaatgcac cttgcatgct
     1441 gatcggcggc tacggcatgg ggttcacctt gagggcggcc atcgccaaca ctgggccgaa
     1501 agccgagctc atcgtgtgcg aactggtccc cgagattatc gaatgggccc gcgggccaat
     1561 gactgatctc gcggccggat gcctcgagga tccgcgcgtg cggctgatga tgggcgacgt
     1621 ggctttcgcc atcgacggtg cgcgcagtga atacgacgcg atcctgcttg atgtcgacaa
     1681 tggccccgat gggctggtgc gcccggacaa caacgggctc tacggcaaga gcggcctgcg
     1741 cgccgcgatg aacgcactga agccgggcgg gatcgtcgcc tactggtcgg cctatgccga
     1801 tccggcgttc acccgccggc tggagcggac cgggtttgac gtggaggaaa tcaccgtgcg
     1861 ggcgcggagc aacggcaagg gcccgcggca tacgatctgg ttcgggcggc ggccgaactg
     1921 agagacgcac cctgcaagct cgtcatgcag gaaacgctgg gtcgcagttt atagttactg
     1981 caacttggct atggcgaacc catgttgacg ggcagcatcc ttggtcgatt cctccgtccg
     2041 attgagagcc ttcgcgattt ccttcagccc cttgcccttg ccggcgagga cctg
//
LOCUS       DATNBV010000284         3639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3661980,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000284 DATNBV010000000
VERSION     DATNBV010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3661980"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VN627_06910"
     CDS             complement(<1..604)
                     /locus_tag="VN627_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HXD03021.1"
                     /translation="MTVEVVILAMIAAFLGLRLYSVLGRRAEHEEETIQGRIEPRGDA
                     GSPGAPRAPRAEAPGLPDRAQLRTAQPVRRELPGVLPAAERGLRDIAVADRRFDALIF
                     LQGARGAYRLILDAFWKGDKDQLAHLCDADVYKRFAEAIDARQASGETLDNRLVRIED
                     AAIVDAELAGNLARITVRFTADIAAVTRDAAGTVVAGSLDD"
     gene            862..1377
                     /gene="secB"
                     /locus_tag="VN627_06915"
     CDS             862..1377
                     /gene="secB"
                     /locus_tag="VN627_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HXD03022.1"
                     /translation="MADEGNVISDLKLDGPAANGQDTGPAAGIISQYIKDLSVENPNA
                     PASYQWQDAPQLDVQFNIQARQIDGEIHEVELKIAMNAKAEGGVAYMIELAYCGLIGM
                     HGLAPEQEHAFTYGEAPRILFPFARRILADAVRDAGYPPVMLEPIDFNGLYLQQLAQA
                     QQTTGEPAGNA"
     gene            1418..2992
                     /gene="murJ"
                     /locus_tag="VN627_06920"
     CDS             1418..2992
                     /gene="murJ"
                     /locus_tag="VN627_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXD03023.1"
                     /translation="MNLLKATGTIGGLTLLSRVLGLVRDSLFARFIGASFASDAFLVA
                     FRLPNMFRALFAEGAFASAFIPMFNQKVADPQGQGLRDGLDFAEAALAVLLPVLLAMT
                     VLLEVFAWPVTFALSGKFNGVSHAQFAFAVELSRYTIPYLMLISLVSLFGGILNSLHK
                     FWVNAAAPILLNLTLIVALLGFHSADPLETARNQAIAVSVSGLLQLLWLAFACRQNGV
                     SLKLRRPQMSPEVKRLLKLIWPAAAGAGAVQINLVISTALAASLLAHGSVTYIYMADR
                     LNQLPLGLIGIGLGTVLLPTISRQLGGGEEAAALETQNRGLDLALLLTLPATVALVVA
                     GEPIAAALFGYGRFTAEDTHFTAQALAAFSIGLPSYILVKVLTPGFYARQDTKTPVRY
                     AMISMVVNLVGNLALIVPLRHMGPPLATAIASTVNVAMLYRTLVARGHFTADARLRRR
                     APRLLLAALAMGVVLWFGQGLLMPYVHGTWPVRFAALAVLVSAGMVVYGLATLVMGAF
                     SKDDIAFLLRRRRSPA"
     gene            3006..>3639
                     /gene="trpS"
                     /locus_tag="VN627_06925"
     CDS             3006..>3639
                     /gene="trpS"
                     /locus_tag="VN627_06925"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXD03024.1"
                     /translation="MRIVSGIQPTGNLHLGNYLGAIRNWVRMQDEHAGGECLYFLADL
                     HAISMPHVPAELAANTREMVAALVACGIDPDRSILFNQAQVPQHAELQWLLNGTARMG
                     WLNRMTQWKDKAGKNREGASVALFTYPVLQAADVLLYQATHVPVGEDQKQHLELARDV
                     AQKFNFDFCPEDAPVFTLPDPIIPPEAARIMSLRDGGAKMSKSDPSEMSRI"
BASE COUNT          559 a         1241 c         1217 g          622 t
ORIGIN      
        1 catcgtcgag cgagccggcg acgacggtgc ccgcggcgtc gcgggtgacc gcggcaatat
       61 ccgcggtgaa gcgcacggtg atgcgggcca ggttgccggc gagttcggca tcgacgatgg
      121 cggcatcctc gatccggacg aggcggttat ccagcgtttc gcccgaggcc tggcgcgcgt
      181 cgatcgcctc ggcgaagcgc ttgtacacgt ctgcgtcgca caggtgggcg agctggtcct
      241 tgtcaccctt ccagaaggca tcgaggatca ggcgataggc gccgcgcgcg ccctggagga
      301 agatgagggc atcgaagcgg cgatcggcca cggcgatatc gcgcaggccg cgttcggcgg
      361 cggggagcac gccgggaagt tcgcggcgga ccggctgggc cgtgcgcagc tgggcgcgat
      421 cgggcaagcc gggagcctcg gcgcggggcg cgcgcggagc gcccgggctg ccggcatcgc
      481 cgcgcggttc gatccgaccc tgaatggtct cctcctcatg ctcggcccgg cggccgagca
      541 cggagtagag ccgcaggccg aggaaggctg cgatcatggc caggatgacg acttcaacgg
      601 tcacgtcagg gtgctttcgt tgggcgggtg cccgcacggc gcgcggactg ccacggactc
      661 aatgtgcctc agataggtaa gcttgacaaa tgaacaacgc atgatcgccg cctatgtgca
      721 tttttcacgt cggagtgtgg tatcgcggat atttggcggg ggacggccgg cgcatccggc
      781 tttcccttgc ggcaccggcc cgctccctgc taggcgcgcc ggctgaacat atccgatcat
      841 ccagtcgaga aagcttatcc catggccgac gaaggcaacg tcatttccga tctcaagctc
      901 gatggccccg ccgccaacgg ccaggacacc ggtccggctg ccggcatcat ctcgcagtac
      961 atcaaggacc tgtcggtcga gaaccccaat gcgccggctt cctatcagtg gcaggacgcc
     1021 ccgcagctcg acgtgcagtt caacatccag gcccgccaga tcgacggcga gatccacgag
     1081 gtcgagctca agatcgcgat gaacgccaag gcggaaggcg gcgtggccta catgatcgag
     1141 ctggcctatt gcggcctgat cggcatgcat ggcctggccc ccgagcagga gcacgccttc
     1201 acctatggcg aggcgccgcg catcctgttc ccgtttgccc gccgcatcct cgccgatgcc
     1261 gtgcgcgatg ccgggtatcc gccggtcatg ctcgagccga tcgacttcaa cgggctctac
     1321 ctgcagcagc tcgcccaggc ccagcagacc acgggcgagc cggccggcaa cgcctgagcc
     1381 cggtgaacgc ctgatcccag tgaacggtgg cctttagatg aacctgctga aggccaccgg
     1441 caccattggc ggactcaccc tgctcagccg ggtgctgggg ctggtgcgcg acagcctgtt
     1501 tgcgcggttc atcggggcga gcttcgcctc ggacgcgttt ctcgtcgcct tccgcctgcc
     1561 caacatgttt cgcgcgctgt tcgccgaagg ggccttcgcc agcgcgttca tcccgatgtt
     1621 caaccagaag gtggcggacc cgcaagggca agggctgcgc gacgggctgg actttgcgga
     1681 ggccgcgctc gccgtgctgc tgccggtgct gctggcgatg accgtgctcc tggaagtctt
     1741 cgcctggccg gtgaccttcg cgctttcggg caagttcaac ggggtcagcc acgcgcagtt
     1801 cgccttcgcg gtggagctgt cgcgctacac gatcccctat ctcatgctga tcagcctggt
     1861 ctcgctgttc ggcggcatcc tcaattcgct gcacaagttc tgggtcaacg ccgccgcgcc
     1921 gatcctgctg aacctgacgc tgatcgtggc gctgctcggc ttccactcgg ccgatccgct
     1981 ggagacggcg cgcaaccagg cgatcgcggt ttcggtctcg gggctgctgc agctgctgtg
     2041 gctggccttc gcctgccggc agaacggggt gagcctcaag ctgcggcggc cgcagatgag
     2101 cccggaggtc aagcggctgc tgaagctgat ctggccggcg gcggcgggcg cgggggcggt
     2161 gcagatcaac cttgtcatct cgaccgcgct ggccgccagc ctgctggccc acggctcggt
     2221 gacctacatc tacatggccg accggctgaa ccagctgccg ctgggcctga tcgggatcgg
     2281 ccttggcacg gtgctgctcc ccacgatctc ccgccagctg ggcggcggcg aggaagcggc
     2341 ggcgctggaa acgcagaacc gcgggctgga cctggctctg ctgctgaccc tgccggcgac
     2401 agtggcgctg gtcgtggcgg gggagccgat tgccgccgcc ctgttcggct atggccggtt
     2461 cacggcggaa gacacccatt tcaccgccca ggccctggcc gccttctcga tcgggctgcc
     2521 gagctatatc ctcgtcaagg tgctgacccc cgggttctac gcgcggcagg atacgaagac
     2581 gccggtgcgc tatgccatga tctcgatggt ggtgaacctc gtcggcaacc tcgccctgat
     2641 cgtgccgctc aggcacatgg ggcccccgct ggcgacggcg atcgcctcga ctgtcaacgt
     2701 ggccatgctt taccgcacgc tggtcgcgcg cgggcatttc accgccgatg cgcgcctgcg
     2761 ccgccgcgct ccgcgcctgc tgctggcggc gctggcgatg ggtgtggtgc tgtggttcgg
     2821 ccaaggcctg ctgatgccct atgtccacgg cacctggccg gtgcgcttcg ccgcgctggc
     2881 cgtgctggtc tcggccggaa tggtcgtcta tgggctcgcc accctggtga tgggcgcctt
     2941 ctcgaaagac gacatcgcct tcctcctgcg gcgtcgccgc tcccctgcct aagagtttcg
     3001 atttcatgcg catcgtttcc ggcatccagc ccaccggcaa tctccacctc ggcaattacc
     3061 tgggggcgat ccgcaattgg gtgcgcatgc aggacgagca tgcgggcggc gaatgcctat
     3121 acttcctcgc cgacctccac gcgatctcga tgccgcacgt gccggccgaa cttgccgcga
     3181 acacccgcga aatggtggcc gcgctggtcg cctgcgggat cgatccggac cgttcgatcc
     3241 tgttcaacca ggcccaggtg ccccagcacg ccgaactgca atggctgctc aacggcacgg
     3301 cccggatggg ctggctgaac cgcatgaccc agtggaagga caaggccggc aagaaccgcg
     3361 aaggcgccag cgtcgccctg ttcacctacc cggtgctcca ggccgcggac gtcctgctct
     3421 accaggccac ccacgtcccg gtgggcgagg accagaagca gcatctcgaa ctcgcccgcg
     3481 acgtcgcgca gaagttcaac ttcgacttct gcccggaaga cgcgccggtc ttcaccctgc
     3541 ccgatccgat catcccgccc gaggccgcgc ggatcatgag cctgcgcgat ggcggggcga
     3601 agatgagcaa gtccgatccc tcggaaatga gccggatca
//
LOCUS       DATNBV010000285         4024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2088231,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000285 DATNBV010000000
VERSION     DATNBV010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088231"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VN627_06930"
     CDS             complement(<1..660)
                     /locus_tag="VN627_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXD03025.1"
                     /translation="MTAALAPEATAAPKVGYPIYDADHHYYETPDAFLRHLDKKYHDK
                     FQYVTLANGRTKLVIDGFLSNYIPNPTFNVVAGPGTHENFYRANNPEGLTMREMGGKP
                     IKSIAAFHNGEAHLKMMDEQGIHAAIIYPTLASIIEVRLGHQVELTQALLHSLNLWVS
                     DEYGFGNGRQFPVGAISLSDPDWAVREMEFLLKAGCKQVLIRPAAVPTETGFRPWGDP
                     AL"
     gene            complement(821..1558)
                     /locus_tag="VN627_06935"
     CDS             complement(821..1558)
                     /locus_tag="VN627_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03026.1"
                     /translation="MDPLFDFTGKVVLVTGGSRGLGKEMVLAFAERGADVIITSRKID
                     NCEAVADQVRGMGRRALAIGAHAAKWPEIDAMLDKAYAEFGRIDIAINNAGMSPAMPS
                     PDVTEALFDSVVGLNFKGPFRLAAVVGKRMMEGDGGVIINVSSSGAIIPMPGIVPYGA
                     SKAALNAMSRSLAKEFAPKVRVNVLSAGPFLTDIAKAWEPEQRETSTNMIGRPGRPEE
                     VVTAALALASPHSSYITGAFYQADGGI"
     gene            complement(1675..2097)
                     /locus_tag="VN627_06940"
     CDS             complement(1675..2097)
                     /locus_tag="VN627_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD03027.1"
                     /translation="MTPDEENLARMAIRHTQSIYNNCGDRGQVEEMVGTFTPDGVMQT
                     NFGIYEGREAILAFVRSVVARSGDADMAGSRHNLTTSRIEFESDTVALGWTYYFVSRR
                     GVTSEDGMYIDRFEKHGDRWLLAHRRVKSLWFLNPTNS"
     gene            2313..3308
                     /locus_tag="VN627_06945"
     CDS             2313..3308
                     /locus_tag="VN627_06945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HXD03028.1"
                     /translation="MTLPATMRVVQADELGLPENFSLVELPVPQPGPGEVLVRMRAAS
                     LGFADVLVASGRYQVKPPVPFSPGSEGSGEVAAVGDGVPRWSVGDRVCVGRFSGAMAE
                     YQLAKPDELHQVPAGMGFLEAAAHRVNYATMLHALKDRARLEPGEILLVLGAAGGVGS
                     AAVQLGKLMGARVIAGASSEAKRDFAKALGADEVLDYSQDNWREALKALTGGRGVNVI
                     ADPVGGNLFEPAFRSLAWKGRHIVIGFAGGDIPRLPANLPLLKGAALIGADIRQFSIY
                     EPELAAANAAQIERWCEEGLRPPLGTVFDLADFQDAMTTAMSGQSLGKVIVRISG"
     gene            complement(3358..>4024)
                     /locus_tag="VN627_06950"
     CDS             complement(3358..>4024)
                     /locus_tag="VN627_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HXD03029.1"
                     /translation="DKIDEFERRYGKPILWAMGATEFCGTIIAWDMDSYRQMWAHKRG
                     SSGRAVAGVELRITDPDTGEELPRNSLGRLSAKVEAVGDDWIVTNDLARIDEDGYVFF
                     EGRADGAIMRGGFKIVPEKVCQTLRQHPDVAEAALIGIPDERLGEVPVAVVEPRPGHS
                     PSAEDLMAFARAHLAAPFIPVQFVFVDRLPCTASTKVAVGEVRKLLFARLGIVDPKAK
                     LPA"
BASE COUNT          652 a         1252 c         1370 g          750 t
ORIGIN      
        1 gagcgcggga tcgccccaag gacggaagcc ggtttccgtc gggaccgccg ccgggcggat
       61 gaggacctgc ttgcagccgg ccttgagcag gaactccatc tcgcgcacgg cccagtccgg
      121 gtcggacagg ctgatcgcgc ccaccgggaa ctggcggccg ttgccgaagc cgtattcgtc
      181 ggaaacccac aggttcagcg agtgcagcag ggcctgggtc agctcgacct ggtgaccgag
      241 ccggacttcg atgatggagg ccagcgtcgg gtagatgatg gcggcgtgga tgccctgctc
      301 gtccatcatc ttgaggtggg cctcgccatt gtggaaggcc gcaatcgact tgatcggctt
      361 gccgcccatc tcgcgcatgg tcagcccttc cgggttgttg gcgcgataga agttctcgtg
      421 cgtgccgggg ccggcaacca cgttgaaggt ggggttgggg atgtagttcg agaggaaccc
      481 gtcgatcacc agcttggtgc gcccgttggc gagggtcacg tactggaact tgtcgtgata
      541 tttcttgtcg aggtggcgca ggaaggcgtc cggcgtctcg tagtagtgat ggtcggcatc
      601 gtagatcggg tagccgacct tgggagcggc ggttgcttca ggggcgaggg cggcagtcat
      661 cggcggggat ctccagagag aaatgatgaa tcatcaaata tgtcctattc ccggatttgt
      721 tcggtgtcaa atctcgtctg cgttacggca gcccgcggat acgcaaacgg gcgcggatcc
      781 gtggatccgc gcccgctgaa cgcctgtatc gcggttgggg tcagatgccg ccgtcagcct
      841 gatagaatgc gccggtgatg taggacgaat gcgggctggc gagggccagg gcggcggtca
      901 ccacttcttc cggccggccg ggtcggccga tcatgttggt gctggtttcg cgctgttcgg
      961 gctcccacgc cttggcgata tccgtgagga acggcccggc cgagagcacg ttgacccgca
     1021 cctttggcgc gaattccttc gcgagcgagc ggctcatggc attgagcgct gccttggaag
     1081 ccccgtaggg cacgatccct ggcatgggga tgatcgcgcc ggaggaggat acgttgatga
     1141 tcacgccgcc atcgccctcc atcatccgct tgccgacgac ggcggcaagg cggaacgggc
     1201 ccttgaagtt cagcccgaca acgctgtcga acagggcttc ggtgacgtcg ggactgggca
     1261 tggccgggct catcccggca ttgttgatgg cgatgtcgat ccggccgaat tcggcatagg
     1321 ccttgtcgag catggcatcg atctcgggcc acttggcggc atgggccccg atcgccaggg
     1381 cacggcggcc catgccgcgg acctggtcgg ccacggcctc gcaattgtcg atcttgcggc
     1441 tggtgatgat cacgtcagcc ccgcgctcag cgaaagcgag taccatctcc ttgcccaggc
     1501 ctcggctgcc accggtgacc aggaccacct tgccggtgaa atcaaacagc ggatccatgc
     1561 cagtcatctc ctcgttttcc cgatacaatc acgaagtcgg cgggcgccgc cgctctcgag
     1621 ctcgacgctg tctggcatct cgctagacac atgtccagtc ttggcgtggg gtgttcagga
     1681 attcgtgggg ttgaggaacc acagcgactt aaccctgcga tgggcgagca gccaacggtc
     1741 gccgtgcttc tcgaaacggt cgatatacat accgtcctcg gaggttaccc cgcgacggga
     1801 gacgaagtag taggtccagc ccagggcgac ggtgtcgctc tcgaactcaa tgcgcgaggt
     1861 tgtcaggttg tgacggctac cggccatgtc cgcatcgccg ctgcgcgcga caactgaccg
     1921 gacgaaggcc aggatggcct cacgcccctc gtagatcccg aaattggttt gcatcactcc
     1981 atccggcgtg aaggtgccca ccatctcctc cacctgcccg cgatcgccgc agttgttgta
     2041 gatcgattgc gtatggcgaa tggccatgcg ggccagattc tcttcgtcgg gggtcatggt
     2101 cttcctctcg gccagttcgc cgcgcagatt gcggcacccg taactggtcg gataccactc
     2161 ggggaatcta tcacgacctt gatgtgggca ttgcgcgtcg catcacgcct gcgatgctat
     2221 gctggccggg ggcttggcga cagcccacgg gtgcggcact ccatgcgcaa cgcgcgtcgc
     2281 cgttggcagg acgcgcatcc cgggagcgcc tgatgactct gcctgccaca atgcgcgtcg
     2341 tgcaagctga cgagcttggc cttccggaaa atttcagcct ggtggaactg ccggtgccgc
     2401 agcccgggcc cggcgaggtg ctggtgcgca tgcgcgcggc gagcctggga tttgccgacg
     2461 tgctcgtcgc ttccgggcgc taccaggtga agccgccggt gccgttcagc ccgggcagcg
     2521 aaggcagcgg cgaagtggcg gccgtgggcg atggtgttcc gcgctggtcg gtgggcgatc
     2581 gcgtctgcgt tggccgtttt tcgggggcca tggccgaata ccagctggcc aagcccgacg
     2641 agctgcacca ggtgccggcg ggcatgggct ttcttgaggc ggcggcccac cgggtcaact
     2701 atgcgaccat gctccatgct ctgaaggacc gggcccggct tgagcccggc gaaatcctgc
     2761 tggtgctggg cgccgcgggg ggcgtgggca gtgctgcggt tcaactaggc aagctcatgg
     2821 gcgcccgggt gattgccggc gcctccagcg aggccaagcg cgatttcgcc aaggccctgg
     2881 gggcggacga ggtgctcgat tacagccagg acaactggcg cgaggcgctc aaggcgctga
     2941 ccggcgggcg cggggtcaac gtcatcgccg atccggtcgg cgggaacctc ttcgaaccgg
     3001 ccttccgcag tctcgcctgg aagggccgcc acatcgtgat cggctttgcc ggcggcgaca
     3061 ttccccggct tcccgccaac ctgccgctgc tcaaaggcgc tgcgctgatc ggcgccgata
     3121 ttcgccagtt ctcgatctac gagccggagc tggccgcggc caatgccgcc cagatcgaac
     3181 gctggtgtga agagggcctc cgcccgccgc tcggtacggt gttcgacctc gctgattttc
     3241 aggacgccat gaccacggcc atgtcgggcc agtcgcttgg taaggtcatt gtccgcatca
     3301 gcgggtagcc ggttggcgct tggcaaaggc cccgtgtgag ccctgcgtct gaggccttca
     3361 ggccgggagc ttggcctttg gatcgacgat acccagccgc gcgaacagca gtttgcgcac
     3421 ctcgcccacg gcgaccttgg ttgacgccgt gcagggcagc cgatcgacga agacgaactg
     3481 gaccgggatg aagggggcgg caagatgtgc ccgggcaaag gccatcaggt cttctgccga
     3541 gggtgaatgg ccggggcgcg gttcgacgac ggccacgggc acttcgccaa gccgctcgtc
     3601 gggaatgccg atgagtgccg cctcagctac gtccggatgc tggcgcaggg tttggcagac
     3661 cttttccggt acgatcttga aaccaccgcg catgattgcc ccgtccgcgc ggccttcgaa
     3721 gaagacatag ccgtcctcgt cgatgcgggc gaggtcattg gtgacaatcc aatcgtcgcc
     3781 cacagcttcg accttggcag acaggcggcc cagcgaattg cggggcaatt cctcgccggt
     3841 atcgggatcg gtgatgcgca gttccacgcc cgccaccgcg cggcccgagc tgccgcgttt
     3901 gtgggcccac atctgccggt agctgtccat gtcccacgcg atgatcgtgc cgcagaactc
     3961 ggtcgctccc atggcccaga gaatgggctt gccgtagcgc cgctcgaact cgtcgatctt
     4021 gtcg
//
LOCUS       DATNBV010000286         6957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2726991,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000286 DATNBV010000000
VERSION     DATNBV010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2726991"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1058
                     /locus_tag="VN627_06955"
     CDS             192..1058
                     /locus_tag="VN627_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXD03030.1"
                     /translation="MSEYEDRYWTSPDGLRLHYRAYGAPAAGERLPVLCLPGLTRNGR
                     DFEDLAPRIAATGREVLCLDLRGRGDSGYAKDSATYNPLQYVEDVEALLAEGGIPRVI
                     AVGTSLGGLMTMLLALRNPAVIGGAVLNDIGPVIEAEGLARIRDYVGSGRSFPTWMHA
                     ARALEEAHGAAFPDFAAADWLRMAKRLLAIGQNGRIAFDYDMKIAEPIAAPGGEGGVD
                     LWPGYMALAGKPLLVLRGELSDLLSAETAAEMARRLPEAEVVTVPRRGHAPTLDEPEA
                     AAAIDRLMARCA"
     gene            1095..2222
                     /locus_tag="VN627_06960"
     CDS             1095..2222
                     /locus_tag="VN627_06960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXD03031.1"
                     /translation="MQVLHLHSGFDAGGKELRAVRLMNLFGPGIEHTVISAQPGALGA
                     SAAVAPGIAVAYPADFPPLAGPLRPGRLHALARAMVGHDLVLSYNWGAMDAVMAHTLF
                     AQALNLPPLVHHEDGFNEDEARRLKPSRNWYRRIGLGKAAGLIVPSQRLERIALDVWH
                     QPRWRVRRIANGIPVGAYARKPRPDALPRVIKREGELWLGTLAGLRAVKNLPRLVRAF
                     AGLPDEWQLVILGEGPERAAIRDEAMRLGVAHRVHLPGFVADPSRVLGLFDLFALSSD
                     SEQFPISVVEAMAAGLAIASPAVGDVAAMIAPDNARFIAAPGDEPGLAAALAALAADP
                     ALRARVGAANRARALAEYDEAAMLAAYRAAYGAALGLPSFP"
     gene            2350..3147
                     /locus_tag="VN627_06965"
     CDS             2350..3147
                     /locus_tag="VN627_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXD03032.1"
                     /translation="MVLRPHSNRPRTPAPTGPAEAGANPGSEVFLREVDDALREQQVV
                     DALKQYGKLAAWAIILFLVVLAGYLWWDHSQNSKAGERGEQLTIALDQVEAGKLDAAR
                     ESLGKLADGKDGVAASAEMMAAGILTQQNKPADAQKAFAAIAADDKAPQPYRDLATIR
                     EVALGFEAMPADQVVARLKPLAVPGKPFFGNAGELLGVAYMKQNRNDLAGPLFAAISR
                     DKTVPDSLRRRARQMAGLLGVDAVDDVNAAAAGLTGGAVAPPAAPAR"
     gene            3175..4521
                     /locus_tag="VN627_06970"
     CDS             3175..4521
                     /locus_tag="VN627_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXD03033.1"
                     /translation="MTSRSSSAALGRILALPAVVGLALALSACGGKKGPTTPTVGNRI
                     PILSRVESGAKVDPSLNQVAVVVPEAVDNPEWPQAGNTPGKSYGNLGLGAAPARLWTA
                     GISGSSKRQRLAASPVIGGGRLFVMDTEGVVHAFDAATGRQQWTEAFRIKGDSAAAVF
                     GGGASYDNNRVYITTGLGEVAALEAATGKLLWRVKPAGPMRGSPTIAFNAVYAMTQDN
                     QVIALNADDGSVLWNVSASTGQSGVFGVAAPAVGQGTVVAGFSTGELVAYRYENGRTL
                     WSDALARTSLSTSVGVLTDIDADPIIDRGQVFALGQGGRMAAYELVTGQRIWELNLAG
                     ISTPAIAGEWIFTLTDDAKLICLARSTGRARWSTQLARFENTKKKKNQIFWTGPVLAG
                     GRLWVANSRGEVMNLSVEDGAVHPFSRVGRGVSLAPVVANRTLYVLDDGGKISAFK"
     gene            4649..5992
                     /locus_tag="VN627_06975"
     CDS             4649..5992
                     /locus_tag="VN627_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXD03034.1"
                     /translation="MTAPGSLGQRPPSDVSAGVGLAGLAGLLGWLAVCRAWPSIAYHL
                     GLATPLEPLSGPCAALAAVLFSGTPMVLWSVLVDKVHRRPSTGIDWTRPRPLDEVVET
                     SITKIAGLWATWAAIGFAYCVARWYWQGQYLFAMEVLGTAALPLFVLSVPYVLWLDRV
                     LVDPRDGAWHFGAMLVGREAWDPEQVKIHWRAWAVKGFFCAFMISILPPGWAELTTVA
                     PEAIFADPFAMTRWLTGLLFVVDVQVAMVGYLLTFKPLDSQIRGANPHLGGWLAALIC
                     YPPFILMGGGSPLDYHPGTADWTVWLANWPAPLLWAWGAMLVALTAIYAWATVAFGLR
                     FSNLTWRGTLTNGPYRWTRHPAYLSKNLFWWLSTLPFLVTTHNVNDAVRNTVLLALVS
                     AVYYWRAKTEEAHLLAEDAKYREYHAWMERAPTLTRALGRLLGKARRRTELQPAE"
     gene            complement(6118..6747)
                     /locus_tag="VN627_06980"
     CDS             complement(6118..6747)
                     /locus_tag="VN627_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXD03035.1"
                     /translation="MPVISVVSRKGGSGKTTIAVHLAVASLGEGRRTCVIDTDPQASA
                     ASWGDWRGSDVPPEVVTCPPLRLRQTVERSLRGGTEIVVIDTPPHGDDAAREAIRAAD
                     LVIIPTRPRSFDLQALEGVTDLISASGKPGFVLLNSVPARATQVLAHAVNYVTCLGLE
                     VCPVHFGERGSFHHYTAAGAVAGEMDPDGKAAAEVDALWGWAKGMVEIG"
BASE COUNT         1042 a         2554 c         2243 g         1118 t
ORIGIN      
        1 ggaagcgcag gatcgtctcc ggaaagggtg ccggttcgac cggatcggcc agccacgggg
       61 ccagttcgag gatgcggggg tcgggccgat agtcggcggc ttgcggttct cggctcatcg
      121 ggcgatagtg ggaactcgcg aaccggcgcg caaggcgcag gtgtccgcac cgagggatta
      181 agcaagccga gatgagcgag tacgaggatc gctactggac cagccccgat gggctgcgcc
      241 tccattaccg cgcctatggc gcccctgccg cgggcgagcg cctgccggtg ctgtgcctgc
      301 ccgggctcac ccgcaacggc cgcgatttcg aggatctcgc gccccggatc gccgccaccg
      361 gccgtgaagt gctgtgcctc gatctgcgcg gccgcggcga tagcggctat gccaaggatt
      421 cggccaccta caacccgctg caatatgtcg aggatgtcga ggccctgctc gccgaagggg
      481 gcattcccag ggtgatcgca gtcggcactt cgctcggcgg gctgatgacc atgctgctcg
      541 ccctgcgcaa tccggcggtg atcggcgggg ccgtgctcaa cgatatcggc ccggtgatcg
      601 aggccgaagg gctggcccgc atccgcgact atgtcggctc cggccgcagc ttccccacct
      661 ggatgcacgc ggcccgcgcg ctggaggagg cccacggggc ggccttcccg gacttcgctg
      721 cggcggactg gctgcggatg gccaagcggc tgctggcgat cggccagaac gggcgcatcg
      781 ccttcgacta cgatatgaag atcgccgaac ctatcgccgc cccggggggt gaggggggag
      841 tggatctgtg gcccggctac atggccttgg ccggcaagcc gctgctggtc ctgcgcggcg
      901 agctgtccga cctgctgagc gctgaaacgg ctgccgaaat ggcccgccgc ctgcccgaag
      961 ccgaagtggt gacggtcccc cgtcgcggcc atgcgcccac gctcgatgag ccggaggccg
     1021 ccgccgcgat cgaccgcctg atggcgcgct gcgcctaggc cgtaaactca taaatgacac
     1081 cacagcctag atccttgcag gtccttcacc tccattcggg tttcgatgcc ggcggcaagg
     1141 agctgcgcgc cgtccggttg atgaacctgt tcggaccggg catcgaacac accgtgatct
     1201 ccgcccagcc cggcgccctc ggcgccagcg ccgccgtggc cccgggcatc gccgttgcct
     1261 atccagccga ctttcccccg cttgccgggc cgctgcgccc ggggcggctc cacgcgctgg
     1321 cccgcgccat ggtcggccac gatctcgtcc tcagctacaa ctggggcgcg atggacgcgg
     1381 tcatggccca caccctgttc gcccaggcgc tgaacctgcc cccgctggtc caccacgagg
     1441 acgggttcaa cgaggacgag gcccggcgcc tcaagccttc gcgcaattgg tatcggcgca
     1501 tcggcctcgg caaggccgcc gggctgatcg tgccctcgca gcggctggaa cggattgcgc
     1561 tggatgtgtg gcaccagccg cgctggcggg tgcggcgcat cgccaacggt attcccgtcg
     1621 gcgcctatgc ccgcaagccc cggcccgacg ccctgccgcg cgtgatcaag cgggaagggg
     1681 agctgtggct cggcaccctc gccggcctgc gcgccgtcaa gaacctgccc cgcctggtcc
     1741 gcgccttcgc cggcctgccg gatgagtggc agctggtaat cctcggcgaa gggcccgagc
     1801 gcgccgcgat ccgcgacgag gccatgcgcc tcggcgtggc ccaccgcgtc cacctgcccg
     1861 gcttcgtcgc cgatccatcc cgggtcctcg gcctgttcga tctcttcgcc ctgtcgtccg
     1921 acagcgagca attcccgatc tcggtggtgg aggccatggc cgccggcctc gccatcgcct
     1981 ctccggcggt cggcgacgtt gccgccatga tcgcccccga caacgcgcgg ttcatcgccg
     2041 cgcccggcga tgagcccggc cttgccgccg cgctcgccgc gctcgccgcc gatcccgccc
     2101 tgcgcgcccg ggtcggggcg gccaaccgcg cccgggccct cgctgagtac gacgaggccg
     2161 ccatgctcgc cgcctaccgc gccgcctatg gcgcggcgct ggggctgccg tcctttccct
     2221 gacgctgcgg ttatgcccgc tattcagccg ggcttcacca cgattgccgt tgaaaagcac
     2281 ccgccatccg ctaagaaccc ggcaatctga agcttacccg cgcccgctat cccggaaaga
     2341 gccgcgaact tggtcctgcg tccccactct aaccgccccc gcacgcccgc gccgaccggc
     2401 cctgccgagg ctggcgccaa ccccggcagc gaggtgttcc tgcgcgaggt ggacgatgcc
     2461 ctgcgcgagc agcaagtggt cgatgcgctc aagcagtacg gcaagctggc ggcctgggcg
     2521 atcatcctgt tcctggtcgt cctcgccggt tatctatggt gggaccattc ccagaactcc
     2581 aaggccggcg aacgcggcga acagctgacc atcgcgcttg atcaggtcga agccggcaag
     2641 ctcgatgccg cccgcgaaag cctcggcaag ctcgccgatg gcaaggacgg cgtcgccgcc
     2701 tccgccgaga tgatggccgc cggcatcctc acccagcaga acaagccggc cgatgcgcag
     2761 aaggccttcg ccgccattgc ggcggatgac aaggcgcccc agccctaccg cgatctcgcc
     2821 acgatccgcg aagtggcgct cgggttcgaa gccatgcccg ccgatcaggt cgtcgcccgg
     2881 ctcaagccgc tggccgttcc cggcaagccc ttcttcggca acgctggcga actgctcggc
     2941 gtcgcctaca tgaagcagaa ccgcaacgat ctcgccggcc cgctgttcgc cgcgatctcg
     3001 cgcgacaaga ccgtgcctga cagcctgcgc cgccgcgccc gccagatggc ggggctgctc
     3061 ggcgtcgatg cggtcgacga tgtcaacgcc gctgccgccg ggctgacggg gggcgccgtg
     3121 gccccgcccg ccgccccggc ccggtaagcc gtgcgtccag gagagatgcc tcacatgacc
     3181 agccgttcgt catcagctgc cctcggccga atcctggccc ttccggccgt cgtgggcctc
     3241 gccctcgcgc tctcggcctg cggcggcaag aaggggccga ccacgccgac cgtgggcaat
     3301 cgcatcccca tcctctcgcg ggtggaaagc ggcgccaagg tcgatccctc gctgaaccag
     3361 gtcgcggtcg tcgtgcccga agcggtcgat aatccggaat ggccgcaggc cggcaacacc
     3421 ccgggcaagt cctatggcaa cctcggcctc ggcgccgcgc cggcccggct gtggaccgcg
     3481 gggatctccg gctccagcaa gcgccagcgc ctcgccgcct cgccggtgat cggaggcggg
     3541 cggctcttcg tgatggatac cgagggcgtc gtccatgcct tcgatgccgc caccggccgc
     3601 cagcagtgga ccgaggcctt ccgcatcaag ggtgacagcg cggcagccgt cttcggcggc
     3661 ggcgcgagct acgataacaa ccgggtctac atcaccaccg gccttggcga ggtcgccgcg
     3721 ctcgaagcgg ccaccggcaa gctgctgtgg cgcgtcaagc ccgccggccc gatgcgcgga
     3781 tcgcccacga ttgccttcaa cgccgtctac gccatgaccc aggacaacca ggtcatcgcc
     3841 ctcaatgccg atgacgggtc ggtcctgtgg aacgtctcgg cgtccaccgg ccagtccggc
     3901 gtgttcggcg tcgccgcccc ggccgtgggc cagggcacgg tggttgccgg cttctccacc
     3961 ggcgaactcg ttgcctaccg ctacgagaac ggccgcaccc tgtggtccga tgccctcgcg
     4021 cgcacctcgc tgtccacctc ggtcggcgtg ctcaccgata tcgacgccga tccgatcatc
     4081 gatcgtggcc aggtcttcgc gctggggcag ggcgggcgca tggccgccta tgaactggtc
     4141 accggccagc gcatctggga actaaacctc gccggcattt ccacccccgc catcgccggc
     4201 gagtggatct ttaccctgac cgacgatgcc aagctgatct gcctcgcccg ctccaccggc
     4261 cgcgcccgct ggtccaccca gctcgcccgg ttcgagaaca ccaagaagaa gaagaaccag
     4321 atcttctgga ccggcccggt cctcgccggt ggacgcctgt gggtagccaa cagccggggc
     4381 gaggtgatga acctctcggt cgaggacggc gcggtccatc ccttctcccg ggtcggcagg
     4441 ggcgtgagcc tcgccccggt cgtggcgaac cgcacgctct atgtgctgga cgacggcggc
     4501 aagatcagcg cgttcaagtg aggctggcaa tctcggcggt gggactggat cagtttaccc
     4561 gctcctcctg agcatgtcga agggctcggg aggaacggat tcggctaggt tcaccctttc
     4621 ccaacccttc gcgcgtaaac cgccgcggat gaccgcgccc ggatcccttg gccagcgccc
     4681 gcccagcgat gtttccgccg gcgtcggcct cgccggcctc gccggcctgc tcggctggct
     4741 ggcggtctgc cgcgcctggc cgtcgatcgc ctaccacctc ggcctcgcca ccccgctgga
     4801 gccgctctcc ggcccctgtg cggccctcgc ggcggtcctg ttctccggca caccgatggt
     4861 cctctggtcg gtgctggtcg acaaggtcca ccgccgtccc tccaccggga tcgactggac
     4921 gcgcccccgc ccgctcgacg aggtcgtgga aacgtccatc accaagatcg ccggcctctg
     4981 ggccacctgg gcggcgatcg gcttcgccta ctgcgtcgcc cggtggtact ggcagggcca
     5041 atatctgttc gccatggagg ttctgggcac ggccgccctg ccgctgttcg tgctctccgt
     5101 cccctatgtg ctctggctcg accgcgtgct ggtagacccg cgcgacggcg cctggcattt
     5161 cggcgccatg ctggtcgggc gcgaggcgtg ggacccggag caggtgaaaa tccactggcg
     5221 tgcctgggca gtcaaaggct tcttctgcgc cttcatgatc tcgatcttgc cccccggctg
     5281 ggccgaattg accacggtcg ctcccgaggc gatcttcgcc gatcccttcg ccatgacccg
     5341 ctggctgacc gggctgctgt tcgtcgtcga cgtccaggtt gccatggtcg gctacctcct
     5401 gaccttcaag ccgctcgatt cccagatccg cggcgccaac ccccacctcg gcggctggct
     5461 cgccgcgctg atctgctatc cgccgttcat cctgatgggg ggcggcagcc ccctcgacta
     5521 tcaccccggc acggccgact ggaccgtctg gctggcaaac tggcccgccc cgctgctctg
     5581 ggcctggggc gccatgctgg tcgcgctcac cgccatctac gcctgggcga cggtcgcctt
     5641 cggcctgagg ttctccaacc tcacctggcg cggcacgctg accaatgggc cgtaccgctg
     5701 gacccgccat cccgcctatc tgtccaagaa cctgttctgg tggctctcca ccctgccgtt
     5761 cctggtgacc acgcacaacg tgaacgatgc ggtgcgcaac acggtgctgc tcgccctcgt
     5821 cagcgccgtc tactactggc gcgcgaagac cgaggaagcc catctcctcg ccgaagatgc
     5881 gaaataccgc gaataccacg cgtggatgga acgggccccg acgctcacgc gcgcgctggg
     5941 ccggctactc ggaaaagccc gccgccgaac cgaactgcaa ccggcggaat aacctcaagt
     6001 ggatgcccgg cagcctcaac cggggtcggg gcgggactgg aatttgccgg ccgtggttct
     6061 gcgagatgga agcatggcac ctgcaccttc tgatcgaagg cgcgtcatct cgctccgtca
     6121 tccaatctct accattcctt tggcccatcc ccacagcgcg tctacctcgg cagccgcctt
     6181 gccatccggg tccatttccc ccgccaccgc ccctgcggcg gtgtagtggt ggaacgatcc
     6241 acgctcccca aagtggacgg gacacacttc caatcccagg cacgttacgt agttgacggc
     6301 gtgagccagc acctgcgttg cccgggcggg aacagagttg aggaggacaa acccgggctt
     6361 gccgctggca gagatgagat ccgtcacgcc ctcaagagcc tgaagatcga acgaccgggg
     6421 gcgcgtggga atgatcacga gatctgcggc acggatcgcc tctcgcgcgg catcgtcccc
     6481 atgcggtggg gtatcgatca ccacgatttc cgtaccgccg cggagcgacc tttcaactgt
     6541 ctggcgcagg cggagcggcg ggcaggtgac cacctcaggc ggaacatcgc tcccgcgcca
     6601 atcgccccaa cttgcagctg acgcctgcgg atcggtatcg atcacacagg tcctccggcc
     6661 ttcgcccagg ctggccacgg caagatgcac agcaatggta gtcttgccgg atccaccctt
     6721 gcgactgacg acggaaatga ccggcattca cggccccgct aacatgggag tttctcgccg
     6781 cggataaatg gcggcaactg ccgggcactg tcaaaggaaa tgcaacagcc gcccttgatc
     6841 agctaggtca ggcgcgggat gtaggacgtg agcccttcgc gccggcctga aggcgttgct
     6901 gaagggtgag cgggcgattg gcgatgaacc cgccgacctc cattgaagcc ctgcggc
//
LOCUS       DATNBV010000287         2560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1753220,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000287 DATNBV010000000
VERSION     DATNBV010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753220"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VN627_06985"
     CDS             <1..150
                     /locus_tag="VN627_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03036.1"
                     /translation="PRMDLKLGDRVFHEKFGTGTITAQEGNKLEIDFDEAGAKRVIDS
                     FVRPA"
     gene            complement(396..653)
                     /locus_tag="VN627_06990"
     CDS             complement(396..653)
                     /locus_tag="VN627_06990"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXD03037.1"
                     /translation="MLVKIRSKGAEMTGIEVLDLSLAGCMVRWRGWRVQDQQRVLISF
                     PALTNLPATILWSEDDRLGLEFERPLHEAVYEHLASGYRIG"
     gene            complement(816..1733)
                     /locus_tag="VN627_06995"
     CDS             complement(816..1733)
                     /locus_tag="VN627_06995"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HXD03038.1"
                     /translation="MTGLLAIPAALALAAAPQPVAPAPAPEPAAVPAPAQIPAQAPSQ
                     APSPDAPAAATPATGTVKTTGYDGGEIVVAARHATREDPLAKLNAQSYEAIQSVDKAF
                     VGPVAMGYKHAVPGPIRSGLRNVLRNLDEPVSAFNYLLQLKPGRAIKSVARFAVNSTF
                     GVAGLVDVAKTKPFNLPYKPNGFANTFACWGIGNGPYFFVPLAGPITLRDLVGLLMDR
                     AALPVAVGKPFSKSYYAIPAGVIDSLNDRIEQDTLINKLREESPDPYVATRDLYLKQR
                     RAEVGAICPRHGDPQPDPSLPPRVGKGQD"
     gene            complement(1864..>2560)
                     /locus_tag="VN627_07000"
     CDS             complement(1864..>2560)
                     /locus_tag="VN627_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HXD03039.1"
                     /translation="KSLKGHGFAAGEIHGDMDQSSRIAELDRFKSGDINILVASDVAA
                     RGLDVKGVSHVFNFDTPWHPDDYVHRIGRTGRAGAKGRAYTFVSPEDAEAIANVEKLT
                     GSAIPSFTYAPEAAAESEEPRRSSRSGSRDEEGDREERPSRARRGREERGEREERPRR
                     EARPERDERPRREARPREDQREEQRDEPRDHRPAYDRRDERRPRRSESSEPPSAPGEW
                     NGPIPGFLSVSAL"
BASE COUNT          359 a          782 c          978 g          441 t
ORIGIN      
        1 ccgcggatgg acctcaagct gggcgaccgg gtgttccacg agaagttcgg cacgggcacg
       61 atcaccgcgc aggaaggcaa caagctggag atcgatttcg acgaggccgg ggccaagcgg
      121 gtgatcgatt cgttcgtgcg cccggcttga ctgttccgtc ctccccaccg gtggggaggt
      181 ggcagcataa ggctgacgga agggggctct cccgccaatg ccgcgcctga ccgatatccc
      241 cctccgtcag ccttcggctg ccacctcccc gtgaacgggg aggattttgg tttcccgctc
      301 agcctcgggc gctgctccgg tgggagagcc ccctccacca cccgcttcgc gggcggtccc
      361 cctcccccct cgtggggagg attttgtttt cgggctcagc cgatccggta gccgctggcg
      421 aggtgctcgt agacggcttc gtgaaggggc cgctcgaatt ccaggcccag ccggtcgtcc
      481 tcggaccaga ggatggtggc ggggaggttg gtcagcgcgg ggaagctgat caggacccgc
      541 tgctggtcct ggacccgcca gccgcgccat ctgaccatgc agccggcgag cgagaggtcg
      601 agcacctcga tcccggtcat ctccgcgcct ttgctgcgga ttttcaccag catcggggtg
      661 gtcgggatag gcgagcgggc ttgtggctgg gcagacatta cgccgctggc atgcggcata
      721 gccgggtaag agtgtcttgc cgcagatcgt caatttgcgt ggttaagaat ttcggccttc
      781 gcgcacacag gcactcggcg ggcgttttct cgccgtcagt cctggccttt gccgacgcgg
      841 ggcggaaggc tcggatcggg ctgcgggtcg ccgtggcgcg ggcagatcgc gccgacttcg
      901 gcgcggcgct gcttgaggta gagatcgcgg gtggcgacgt agggatcggg gctttcctcg
      961 cgcagcttgt tgatcagggt gtcctgctcg atccggtcat tgagggaatc gatcacgccg
     1021 gcggggatgg catagtagga cttgctgaac ggcttgccga cggcgaccgg aagggcggcc
     1081 cggtccatca gcaggccgac gagatcgcgc agggtgatcg ggccggcgag cggcacgaag
     1141 aaataggggc cgttgccgat gccccagcag gcgaaggtgt tggcaaagcc attgggcttg
     1201 tagggcaggt tgaacggctt ggtcttggcg acatcgacga gaccggcaac gccgaaggtg
     1261 gagttcaccg cgaagcgggc gaccgacttg atcgcgcggc ccggcttgag ctggagcagg
     1321 tagttgaagg cgctgaccgg ctcatcgagg ttgcgcagga cgttccggag gcctgagcgg
     1381 atcgggcccg ggactgcgtg cttgtagccc atggcgacgg ggccgacgaa ggccttgtcg
     1441 accgactgga tggcctcgta gctctgcgcg ttgagcttgg cgagcggatc ttccctcgtg
     1501 gcgtggcggg cggcgacgac gatttcgccg ccatcatagc cggtggtctt gaccgtgccg
     1561 gtggcggggg tggccgcagc gggggcatcg ggcgacgggg cctgcgacgg ggcctgcgcc
     1621 gggatttgcg ccggggctgg aacagcggcg ggctcgggcg cgggagccgg ggcaacgggc
     1681 tgcggggcgg cggccagcgc gagggcggcg gggatggcga gcaggccggt catccgccat
     1741 gcgctccggc aggttggcag gtcccgatca tgatacccct tccgcgcgga ctggccggag
     1801 ggcgcgtcct gtcatcccgc cgtctagcgg cagtcaatcg caggtcgcaa gcgcggcctg
     1861 cgattaaagc gcggaaaccg aaaggaagcc ggggatcgga ccgttccatt cgcccggcgc
     1921 gctgggcggc tcggacgatt cggaacggcg ggggcggcgt tcatcgcggc gatcgtaggc
     1981 gggacggtga tcgcgcggtt cgtcgcgttg ttcctcgcgt tggtcttcac ggggacgtgc
     2041 ttcccggcgc ggccgttcgt cacgctcagg gcgagcttcg cggcgggggc gttcctcgcg
     2101 ttcgccgcgc tcctcacggc cccggcgcgc gcggctggga cgttcctcgc ggtcaccttc
     2161 ctcatcgcgg ctgccgctgc ggctggaacg gcggggctcc tcgctttcgg cagcggcttc
     2221 gggcgcatag gtgaacgagg ggatcgccga gccggtcagc ttctcgacat tggcgatggc
     2281 ctcggcatcc tcgggcgaga cgaaggtata ggcccggccc ttggcgccgg cgcggccggt
     2341 gcggccgatg cggtggacat agtcgtccgg gtgccagggc gtgtcgaagt tgaagacgtg
     2401 gctcacgccc ttcacgtcga gcccgcgggc ggcgacgtcg gaggcgacga ggatgttgat
     2461 gtcgcccgac ttgaagcgat cgagctcggc gatgcgcgag gactggtcca tgtcgccgtg
     2521 gatctcgccc gcggcgaagc catggccctt caggctcttg
//
LOCUS       DATNBV010000288         2855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1451163,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000288 DATNBV010000000
VERSION     DATNBV010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451163"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VN627_07005"
     CDS             complement(<1..712)
                     /locus_tag="VN627_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXD03040.1"
                     /translation="MQRHLRAGFAKRLKADASYVTDADLAAERTLRALIGQRFPRYGI
                     VGEEFPPVNAGASYQWILDPIDGTLSFTHRLPFFGTIIGLHHEGVPIVGVIDHPALDL
                     CYSAAVGLGAWCNGRRLHVTDIPASARPREVISASDRPRFVRCRRGQAFDRLMRIHPQ
                     VRGYADCIAHTLAAEGKIGAVVDFGLRLWDIGATQVLIEEAGGRFSCVHRSRDRDGEL
                     YGIIAGKPSVVRWLERLFR"
     gene            951..2471
                     /locus_tag="VN627_07010"
     CDS             951..2471
                     /locus_tag="VN627_07010"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXD03041.1"
                     /translation="MSSGPVTTDSARDAADLARFGYKQELKRTLGTFSSFAVAFSYIS
                     PSTGIFTLYALGLSFIGPVFFWTWPLVALGQFMIALNFAELSSHYPVAGSVFQWTKYL
                     AGRRYAWFNGWIYLFAGVITVTAVVVTLPIALIPVFNGLGWPMENSLENQRVIAVNTL
                     ILITVLNIFGVKLVSIVNNTGVVFEILGMVVFALILALFHNRQGVGVVFNSGGQDVNF
                     STFMAAMFMSLFVIYGFDTASTLAEETRDPRREAPKAVLGSIIGAFVIGTIFLWGTLM
                     AIPDLKEAIAKGMGPAQIIEASFSTFWATVYLLVVAAAIFVCCLSIMTSTIRLAFGMA
                     RYNQLPLSQPLAKVSPALHTPIGASVAVAILAAIPLLQYAGAAYIAIAATGMIYLSYF
                     LGNIATLRARLGGWPRTAAPFRLGGWGVPVNILALVWGGSMLINFAYPRACCNPKPVE
                     TLSGDVQLLNFHSEFLNHTPVLWTVFGIILLVGAIYYFAIQVRKPFEAVVPPEEPA"
BASE COUNT          459 a          938 c          972 g          486 t
ORIGIN      
        1 atcggaagag gcgctccagc catcgcacca cgctgggctt cccggcgatg atgccgtaga
       61 gctctccatc gcggtcacgg gagcggtgga cacaggagaa ccgcccgccg gcctcctcga
      121 tcaggacctg ggtggccccg atgtcccaca gccgcagccc gaagtcaacc accgcgccga
      181 tcttgccttc ggccgcgagg gtgtgggcga tgcagtcggc gtagccgcgg acctgggggt
      241 ggatccgcat caggcgatcg aacgcctgcc cccgacggca gcgcacgaac cggggccggt
      301 ccgaggcgga gatcacctca cggggccggg ccgacgcggg gatgtccgtc acgtgcaggc
      361 ggcgtccgtt gcaccaggcg ccgagcccga ccgcggcgct gtaacagaga tcgagcgcgg
      421 ggtggtcgat cacgccgaca atcggcacac cctcgtggtg caggccaatg atggtaccga
      481 agaacggcag gcggtgggtg aaactgaggg tcccgtcgat cgggtcgagg atccactggt
      541 aggacgcgcc ggcgttcacg gggggaaatt cctcgcccac gatgccgtac cggggaaagc
      601 gctgcccgat caacgcgcgc agggtgcgtt cggccgccag gtcggcatcg gtgacgtagc
      661 tggcgtcggc cttgagccgc ttggcgaacc cggcgcggag gtgccgttgc accaccaccc
      721 gggaccggcg cgcgaacgcc cgcgccgccc gcagcaggcg ccgccgctcc gcgagggaga
      781 cgcgaggggg gggaggcaca ggcgctaatg tagcgtgcgc ggccgggggc cggaacaccg
      841 cgcgggccgc gggcgatctg gacatcgttc ccgccgcgcg gtaccctatc ggggccccac
      901 acgcagtgac tcccgcaggt cgcgaccgat cccgcacaag gagggtctcg atgtcgagcg
      961 gcccggtaac gacggatagc gcccgcgatg ccgccgatct ggcgcgattc ggctacaagc
     1021 aagagctcaa gcgcacgttg ggcacgttca gctccttcgc cgtcgcgttc agctacatct
     1081 cgccctccac cggcatcttc acgctctacg ccctcggcct ctccttcatc gggcccgtct
     1141 tcttttggac ctggccgctg gttgccctgg gccagttcat gatcgcgctc aactttgccg
     1201 agctgtcgag ccactacccg gtggccgggt cggtgttcca gtggaccaag tacctggcgg
     1261 gccggcggta cgcgtggttc aacgggtgga tctacctgtt cgccggcgtg atcaccgtga
     1321 ccgcggtagt ggtgacgctg ccgatcgcgc tgatcccggt cttcaacggg ctgggatggc
     1381 cgatggagaa ttcgctggaa aaccagcggg tgatcgcggt gaacacgctc atcctgatca
     1441 ccgtgctgaa tatcttcggg gtgaagctgg tctccatcgt gaacaatacc ggcgtcgtgt
     1501 tcgagatcct gggcatggtg gtgttcgcgc tcatcctggc gctgtttcac aaccggcagg
     1561 gcgtcggcgt ggtgttcaac agcggcgggc aggacgtgaa cttcagcacc ttcatggcgg
     1621 cgatgttcat gagcctgttc gtcatctacg gcttcgacac cgcgtcgacc ctggcggagg
     1681 aaacgaggga cccccgtcgg gaagcgccga aggcggtgct ggggtcgatc atcggggcgt
     1741 tcgtgatcgg caccatcttc ctgtggggca cgctcatggc gattcccgac ctgaaggagg
     1801 ccatcgccaa gggcatggga ccggcccaga tcatcgaggc gagcttctcc acgttctggg
     1861 ccacggtcta tctgctggtg gtcgcggccg cgatcttcgt gtgctgcctg tcgatcatga
     1921 cctcgaccat ccggctggcg ttcggcatgg cgcgctacaa ccaattgccg ctgtcgcagc
     1981 ccctggccaa ggtcagcccg gccctgcata cgccgatcgg cgccagcgtg gccgtcgcga
     2041 ttctcgcggc catcccgctg ctccagtacg ccggggccgc ctatatcgcg atcgccgcca
     2101 ccgggatgat ctatctcagc tactttctcg gcaacatcgc gacgctgcgc gcgcggctgg
     2161 gcggctggcc gcggacggcc gcgcccttcc ggctgggcgg ctggggcgtg ccggtcaaca
     2221 tcctcgccct ggtctggggc ggctcgatgc tgatcaactt cgcctacccc agggcctgtt
     2281 gtaatccgaa gccggtcgag accctatcgg gcgacgtcca gctgctcaat ttccacagcg
     2341 agttcctcaa tcacaccccg gtgctgtgga ccgtgttcgg gatcattctc ctggtgggag
     2401 cgatctacta cttcgccatt caggtacgca agccgttcga ggcagtggtg ccgccggagg
     2461 agcccgcgta gcgggtcgga gcgggatggg attccataaa acgagaggcc cccgtgttgc
     2521 ggcctagatg acgcgcgggg gccgagcagg aatcggggtt gtcggcatcg acttaccacg
     2581 gaaacacgga gagcacggaa acatcacgga gacaactagg ggtaaaacag cacacccaca
     2641 tactccttgc tttctccgtg acgtttccgt gcctccgtgt ttccgtggtg aataaaagcc
     2701 aaaaaacgcc ccccggaccc aggtccgggg ggcgttcgct tttcggatgc ggccggtcag
     2761 gccacgagat cgcgcagccg gtcgccggtc tcgccttcgc gcaggcggag gagggcgcgg
     2821 tcccggagct ggcggatccg ctcgcgggtg acgcc
//
LOCUS       DATNBV010000289         2478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1154776,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000289 DATNBV010000000
VERSION     DATNBV010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154776"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VN627_07015"
     CDS             <1..1027
                     /locus_tag="VN627_07015"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXD03042.1"
                     /translation="EAMRLFATGVAPSQFHARFDAQFGHRLKWVILAEDLPDEANRVE
                     LHPELTDADGIPAARIVYRTSASVRRALDHSVAQASEILRDAGAYAIEATNPAPVGAH
                     LMGTARMGDDPAGSVVDRWCMAHDIPNLGVIDGSVFVTAGTVNPTSTICALALRAADH
                     LVRNRGSLRPPARSYHRIPEGLPCRNVAPDAQPERVGALTADQHARLATIADALIPAV
                     DELPAGGAMIDGTALQRLFAARPDMLPELRRILAGPERDPVSLVDHLAEAAPLDRELL
                     LTAIAGVYFLDPRVRARIGYDGQAAIPQSPDRFPAYLAEGLLDHVFADGWHERWEAGA
                     SSVDAPA"
     gene            complement(1039..>2478)
                     /locus_tag="VN627_07020"
     CDS             complement(1039..>2478)
                     /locus_tag="VN627_07020"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD03043.1"
                     /translation="LLCITMEGVEDIPGVVMKEGLTWDWETFPEYLDALEARERDIDV
                     GVLVPHSAVRVYAMGKRGADRVPADDNDLAAMHELISEAVRAGALGFGSTRTAFDRRA
                     DGEHVPSFDAASEELANAARAVRDGGGGVLQILPDLGTFGSSPETCFSLMKVMSEASG
                     LPVTYTMSQVKEHPDHWRALLRLTQEHNRAGGALVHPQFFPRPVGMLAGLQLTSNPFV
                     YCPSYQAIAHLPLAERVTEMRRPDVRARIVSEEPGDTLLPMTAMTRQWQNMYRLGDPP
                     CYEPDPETSVAAMARREGRPPEEIAYDLLLENDGHALLLVAIANYAYGSLDYLIEMMR
                     DPNCVIGLGDGGAHYGLICDSSYPTFVLTHWARDRQGDRLTLEEAVKALTSVPAGVVG
                     LGDRGILAPGYKADINVLDHSGMALHAPRIVDDLPAGGRRLDQSASGYRWTLVSGEVI
                     LRDDQSTGAYPGRLVRGRRTVAAVPEVAC"
BASE COUNT          402 a          839 c          823 g          414 t
ORIGIN      
        1 cgaggcaatg cgcttgttcg ccaccggtgt cgcaccttcg cagttccatg cccggttcga
       61 tgcgcaattc ggtcatcggt tgaaatgggt gatcctggca gaggacctgc cggacgaggc
      121 caatcgcgtg gaactccacc ccgagctcac cgatgcggac ggcattccgg cagcccggat
      181 cgtttatcgc accagcgcca gcgtcaggcg cgcgttggac cacagcgttg cccaagcttc
      241 ggagattcta cgtgatgcag gcgcctatgc catcgaggcg accaatcccg cgcccgtcgg
      301 cgctcacctg atgggcacgg ccaggatggg cgatgaccct gccggttccg ttgtggaccg
      361 ctggtgcatg gcgcacgaca tccccaacct gggggttatc gatggcagtg tgtttgtcac
      421 cgccgggacg gtcaatccca cgagcacaat ctgcgcgctg gccttgcggg ctgccgacca
      481 cctggttcgc aatcgcggct cgcttcgccc gccggcgagg agctaccacc gcatcccgga
      541 agggttaccg tgcaggaacg tcgcaccgga cgcccagccg gagcgtgtcg gcgctttgac
      601 tgcggatcag cacgcccgct tggcgacgat tgccgatgcg ctcatccccg ccgtggacga
      661 gctccccgca ggcggcgcca tgatcgacgg cacggcactg cagcgcctgt ttgcggcgcg
      721 cccggatatg ctgcccgaac tgcgcaggat ccttgccggc cccgagcgcg atccggtttc
      781 ccttgtggat cacctcgccg aggccgcgcc cttggaccgg gaattgctgc tgacggccat
      841 cgcgggcgtc tacttcctcg atccccgggt gcgggcgcga attggctatg acgggcaagc
      901 tgccattccg cagtcgcccg accggtttcc tgcctatctc gccgagggcc tgctggacca
      961 cgtattcgct gatggctggc acgaacggtg ggaagccggc gcgtccagtg tggacgcgcc
     1021 ggcctgaggc gatcggtgct agcaggcgac ttcgggaacg gcggccactg tccgccgccc
     1081 gcggaccaga cggccgggat aggccccggt cgactggtcg tccctgagga tgacctcgcc
     1141 cgagaccagg gtccagcgat agcctgaggc gctctggtcg aggcggcgcc cacccgccgg
     1201 caggtcatcg acgatgcggg gggcgtgcag ggccataccc gaatggtcga ggacattgat
     1261 gtcggcctta tagccggggg ccagtatccc gcggtcgccc aggccgacca ctccggccgg
     1321 gacggaggtc agagccttga ccgcttcttc cagtgtcagc cggtcaccct gccgatcgcg
     1381 ggcccagtgg gtcaggacga aagtgggata gctggaatcg cagatcaggc catagtgcgc
     1441 tccgccgtcg ccgaggccga tcacgcagtt cggatcgcgc atcatttcga tcaggtagtc
     1501 gagcgagcca taggcgtagt tggctatcgc aaccagcaac agcgcgtgcc cgtcgttctc
     1561 gagcagcagg tcgtaggcga tctcttcggg ggggcgaccc tcgcgccggg ccatggccgc
     1621 gacgctggtt tcaggatcgg gttcgtaaca gggcgggtcg cccaggcggt acatgttctg
     1681 ccactgccgg gtcatcgcgg tcatcggcag cagggtatcg ccaggctcct cgctcacgat
     1741 cctggcgcgc acgtccggcc ggcgcatttc ggtcacgcgc tcagccagcg gcagatgggc
     1801 aatggcctgg taggacggac aataaacgaa gggattgctg gtcagctgga gcccggccag
     1861 catccccacc gggcgtggga agaactgcgg gtggaccaag gcgccccccg cccggttgtg
     1921 ctcctgcgtc aggcgcagca acgcccgcca gtgatcggga tgctccttga cctgcgacat
     1981 cgtgtaggta accggcaagc ccgaagcctc actcatgacc ttcatcagcg agaagcacgt
     2041 ctcgggcgag gagccgaacg tgccgaggtc gggcaggatc tggagcaccc cgcctccacc
     2101 gtcgcgaacc gcccgcgccg cgttggcaag ctcttcggat gccgcgtcga agctgggcac
     2161 gtgttcgcca tcggcgcgcc gatcgaacgc ggtgcgggta gaaccgaagc cgagtgcccc
     2221 ggcacgcacg gcctcgctga tcagctcgtg catcgccgcc aggtcgttgt cgtcagcagg
     2281 cacgcggtcg gccccgcgtt tgcccatggc gtagacgcga accgcggaat gcggcaccag
     2341 cacgccgacg tcgatgtcgc gctcccgggc ttcaagagca tcgaggtatt ccgggaaagt
     2401 ctcccagtcc caggtcagac cttccttcat caccacgccc ggtatgtctt ccaccccctc
     2461 catcgtgatg cacaggag
//
LOCUS       DATNBV010000290         2106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2385273,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000290 DATNBV010000000
VERSION     DATNBV010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385273"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1718
                     /locus_tag="VN627_07025"
     CDS             <1..1718
                     /locus_tag="VN627_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HXD03044.1"
                     /translation="LPESAKAQQAPAAPTREELQAQAPAAEIPRQSRLTIEDTVERGP
                     CALADPAMAGVKVNFANVSFTGLPDVPPGALDEAWRDLAGREQPIATLCEVRDRAATM
                     LRRWGFLATVQVPPQRIEAGGTVQFNVLAAKLVAVEVRGRPGHAERQIAAHLRQLTKR
                     PWFNTQEAERQLLLLRDLPGFDVRLTLRSAHAGPGEVIGEVMVQRRPVELIVGGQNLS
                     SKATGREGVMAQLTLNDLTGMGDRTVVSVYNTVQTGEQTVLQLAHDFAIGPDGLRLGG
                     RFVYGRARPDIAGGAFVSHTWIGAAELSYPFLRKQTATLLGAAGFEAVDQLIDFGGTR
                     LSADRLRIAYGRLDGRLVDSASLAGREGFTLAEPRLGLSGSLEVRKGLAGLGASSACV
                     PLTVCLPPHVPITNLAANPQGALVRAEATVEFRPARAVTLVLRPRAQYSSSQLLSYEQ
                     FTLGNYTVGRGYDPGAVQGDKGVALGAELRLGRLRPRSAEDIALQPYGFVDAGWAWTN
                     DNGLTPIRHLVSAGGGVRARWGDRGDFNLLLAVPLERAPGQSQLGPARVLLTFTTRLV
                     PWSVQ"
     gene            1715..>2106
                     /locus_tag="VN627_07030"
     CDS             1715..>2106
                     /locus_tag="VN627_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03045.1"
                     /translation="MNRHNDGRRALLAGTALAATLGLAGWAGQAMAQGFNGTGTVVGG
                     TSATIAPGNADITINAAESIINWNVTGPAGATDVAFLPLNKTVTFHETVSPGFTDYTV
                     LNRVFPVTASAVGPGVPLNATINIGGTI"
BASE COUNT          303 a          825 c          667 g          311 t
ORIGIN      
        1 ccctcccgga atcggccaag gcccagcaag caccagccgc gcccacgcgc gaggaactgc
       61 aggcccaggc ccccgccgcg gaaatcccgc gccagagccg gctgaccatc gaggacacgg
      121 tcgaacgcgg cccctgcgcg ctggccgatc cggcgatggc cggggtcaag gtcaacttcg
      181 cgaatgtcag cttcaccggc ctgcccgatg tcccccccgg cgcgctcgac gaagcctggc
      241 gcgatctggc cgggcgcgag cagcccatcg ccaccctgtg cgaagtgcgc gaccgcgccg
      301 ccaccatgct gcgccgctgg ggcttcctcg ccacggtcca ggtcccgccc cagcggatcg
      361 aggccggggg cacggttcag ttcaacgtgc ttgccgccaa gctggtcgcc gtcgaagtcc
      421 gcggccgccc cggccatgcc gagcgccaga tcgccgccca cctgcgccag ctgaccaagc
      481 gcccctggtt caacacccag gaggccgagc gccagctcct cctcctgcgc gacctgcccg
      541 gcttcgacgt gcgcctcacc ctgcgctccg cccatgcggg cccgggcgag gtcatcggcg
      601 aggtcatggt ccagcgccgc ccggtcgaat tgatcgtcgg cggccagaac ctctcgtcga
      661 aggccaccgg gcgtgagggc gtcatggccc agctgacgct caatgatctc accggcatgg
      721 gtgatcgcac cgtggtcagc gtttacaaca ccgtgcagac gggcgagcag acagtgctcc
      781 agctcgccca cgatttcgcc atcgggcctg atggcctgcg cctcggcggg cgcttcgtct
      841 atggccgggc ccgtcccgac atcgcgggcg gcgccttcgt ctcgcacacc tggatcggcg
      901 cggccgaact gtcctatccg ttcctgcgca agcagaccgc caccctgctc ggtgccgccg
      961 ggttcgaagc ggttgaccag ctgatcgact tcggcggcac ccgtctctcc gccgatcgcc
     1021 tgcgcatcgc ctatggccgg cttgacggcc gcctggtcga cagcgccagc ctcgccgggc
     1081 gtgagggctt caccctggcc gagccgcgcc tgggcctgtc gggcagcctg gaagtgcgca
     1141 agggcctcgc cggcctcggc gccagcagcg cctgcgtgcc gctgaccgtt tgcctgccgc
     1201 cgcatgtccc gatcaccaac cttgccgcca acccgcaagg cgcgctggtc cgggccgagg
     1261 cgacggtgga attccgcccg gcccgcgcgg ttacgctcgt cctgcgcccc cgcgcgcagt
     1321 acagctcgtc ccagctcctg agctacgagc agttcaccct gggcaactac accgtcggcc
     1381 gcggctatga tccgggcgcg gtccagggcg acaagggcgt ggcgctgggt gccgaactgc
     1441 gcctcggccg cctgcgcccg cgctcggctg aagacatcgc cctccagccc tacggcttcg
     1501 tcgatgccgg ctgggcctgg accaacgaca acggcctgac cccgatccgc catctggtct
     1561 cggccggcgg cggcgtccgc gcgcggtggg gcgatcgggg ggatttcaac ctcctcctcg
     1621 ccgtcccgct cgaacgtgcc cccggtcagt cccagctcgg accggcccgc gtcctgctca
     1681 cctttaccac ccgccttgtc ccctggagcg tgcaatgaac cgccataatg atggacgccg
     1741 ggccctgctc gccggaaccg cgctcgccgc aacactcggg cttgccggct gggccggcca
     1801 ggccatggcg cagggcttca acggcacggg cactgtcgtc ggcggcacct cggccacgat
     1861 cgccccgggc aatgccgaca tcacgatcaa cgccgccgaa tctatcatca actggaacgt
     1921 gacgggaccg gctggcgcca ccgacgtcgc cttcctgccg ctgaacaaaa ccgtcacgtt
     1981 ccacgagact gtttcgccgg gcttcaccga ctacaccgtg ctcaaccggg tattcccggt
     2041 aacggccagt gctgttgggc ccggcgttcc gctcaacgcc acgatcaaca tcggcggcac
     2101 gatcaa
//
LOCUS       DATNBV010000291         3084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2385363,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000291 DATNBV010000000
VERSION     DATNBV010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385363"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VN627_07035"
     CDS             <1..488
                     /locus_tag="VN627_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HXD03046.1"
                     /translation="AIHRAAESLPVSYSSELWCEPGRALCAEYNSIVVKVEKRRGDEL
                     YINDGAYGALFDAAHLDWRFPVKCLNGRDGEEEEFAFYGPTCDDGDYMKGPFALPADI
                     AAGDFIEIGMLGAYGAAMKTAFNGFDAVAVIEVGDEPMASQYLGNRRDPRVSDNVVSL
                     R"
     gene            complement(646..2052)
                     /locus_tag="VN627_07040"
     CDS             complement(646..2052)
                     /locus_tag="VN627_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HXD03047.1"
                     /translation="MSQNRPLYLGPRLRRLRRELGLTQQAMAGDLDISASYVALLERN
                     QRPVTADLLLRLARTYRLDVSELAGEDAADDARKVTEILRDPLLADIDLPALEVADLA
                     TSFPGVTEALLRLHGAYLREQQALADRQLGRDDEAPDAPDPVRAAQEFLAAEHNHFPT
                     LDARAEELAAQIAEDGTATEWLGRRKGVRVRFLPPDVLVESVRRFDRHNQQLLIDDTL
                     DMTTRKFQIAVHVAWSALREDINAIARAAQLPDRSATALVRRNLAAYAAGALIMPYDR
                     FAKAAEARGYDIAALCGLFGASFEQVAHRLTTLGRPGLEGVPFFFLRVDPAGNVSKRL
                     DGAGFPFARQGGGCPLWSVHETFRRPGEILTQWLELPDGQRFFSIARTVGGQARHLGA
                     PATTRAVALACSAQHASRLAYSGGVDPATIAATPIGIGCRLCQRATCEARALPPIGRE
                     LAADDQRRGTAPYLFSEG"
     gene            2211..>3084
                     /locus_tag="VN627_07045"
     CDS             2211..>3084
                     /locus_tag="VN627_07045"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HXD03048.1"
                     /translation="MTYSQTIAAANQTISPNAGTWNGIDAANVARMRLQNRFLTGIDI
                     ARYTAKIMRADMAAYDADPANYTQSLGCWHGFIGQQKMISIKKHFGTTKGRYLYLSGW
                     MVAALRSEFGPLPDQSMHEKTSVPMLIEELYTFLKQADARELGGLFRELDAAKAAGDA
                     VGIHRTLGQIDAFQTHVVPIVADIDAGFGNAEATYLLAKKFIEAGACCIQIENQVSDE
                     KQCGHQDGKVTVPHEDFIAKIRAVRYAFMELGVDDGVIVARTDSLGAGLTKQIAFVRE
                     AGDIGDQYNAFLDCE"
BASE COUNT          525 a         1017 c         1084 g          458 t
ORIGIN      
        1 gcgcgatcca ccgcgcggcg gaatcgctgc cggtgtccta ctcgtccgag ctgtggtgcg
       61 agcccggccg ggccctgtgc gcggaataca actcgatcgt ggtcaaggtg gaaaagcgcc
      121 ggggcgacga gctctacatc aacgacggcg cctatggcgc gctgttcgat gccgcgcatc
      181 tggactggcg ctttccggtg aagtgcctga acgggcgcga cggcgaggag gaagagttcg
      241 ccttctacgg gccgacctgc gacgatggcg actacatgaa gggccccttc gcccttcccg
      301 ccgacattgc cgcgggcgac ttcatcgaga tcggcatgct cggcgcctat ggcgcggcta
      361 tgaagaccgc gttcaacggc ttcgatgccg tggccgtgat cgaggtgggc gatgagccga
      421 tggccagcca gtacctcggc aaccgccgcg atccgcgggt ttcggacaac gtggtgtcgc
      481 tgcgttgaag tgagggaaag gccctccggc ctttccctgc ggcaccggcc ccctcccccg
      541 gcccgaccaa ccgacatggt acactaagcg ggtggtcggg ccgggggagc gggctggtgc
      601 cgcaagccct ccacggatgt ggcgggcaca ccccacaaag actcctcacc cctcgctgaa
      661 caggtagggc gcggttcccc gccgctggtc gtcggcggcc agttcgcggc cgatgggcgg
      721 aagggcccgg gcctcgcagg tggcgcgctg gcacaggcgg cagccgatgc cgatcggggt
      781 ggcggcgatc gtcgcgggat cgaccccgcc actgtaggcg aggcgcgagg catgctgcgc
      841 ggagcaggcg agcgccacgg cgcgggtcgt ggccggggca ccgagatggc gggcctgtcc
      901 gccgacggtg cgcgcaatcg agaagaagcg ttggccatcg ggcagttcga gccactgcgt
      961 caggatctcg cccgggcggc ggaaggtttc atggaccgac cacagcggac agcccccgcc
     1021 ttgccgcgcg aagggaaagc cggcgccgtc gagccgcttg gagacgttgc cggccggatc
     1081 gacccgaagg aagaagaacg gcaccccttc cagcccgggg cggccaagcg tggtcagccg
     1141 gtgggcgacc tgctcgaacg aggcgccgaa caggccgcac agggcggcaa tgtcatagcc
     1201 ccgggcctcg gcggccttgg cgaaccggtc atagggcatg atcagcgcgc cggcggcata
     1261 ggcggcgaga ttgcggcgga ccagcgcggt ggccgagcgg tccggcagct gggcggcgcg
     1321 ggcgatggcg ttgatgtcct cgcgcagggc gctccacgcg acgtggacgg cgatctggaa
     1381 ctttcgcgtg gtcatgtcga gcgtgtcgtc gatgagcagc tgctggttgt gccggtcgaa
     1441 ccggcggacc gattccacca gcacgtccgg cggcaggaac cggacgcgca cgcccttgcg
     1501 ccggccgagc cattcggtgg cggtgccatc ttcggcgatc tgggcggcga gttcctcggc
     1561 ccgggcatcg agggtgggaa agtggttgtg ttcggcagca agaaattctt gcgccgcgcg
     1621 gaccggatcg ggggcatccg gcgcctcgtc gtcgcggccc agctggcgat cggccagagc
     1681 ctgctgttcg cgcagatagg cgccgtgaag ccgcagcagg gcttcggtga cgccggggaa
     1741 ggaggtggcc agatcggcca cctcgagggc gggcaggtcg atgtcggcaa gcagcggatc
     1801 gcgcaggatt tcggtgacct tgcgggcatc gtcggccgca tcctcgccgg ccagttcgct
     1861 gacatcgagg cggtaggtcc gcgccaggcg caggagcagg tcggcggtga ccgggcgctg
     1921 gttgcgctcg agcagggcga cgtaggaggc ggagatatcg agatcgccgg ccatggcctg
     1981 ctgggtgagg ccgagctcgc ggcgcaagcg gcgcaggcgg ggcccgagat agagggggcg
     2041 attctggctc acggtgaaca ccttacaagc tgcaagcgta cgcttgtcat agctgcacaa
     2101 ctttacaaag aatagctgta aagtttgaca agcgcacccc gtagagcctg ccgggaccaa
     2161 ggttcccgca ttagtggccg gccatcaacc gcctacgcaa ggattcgccc gtgacctact
     2221 cgcagaccat cgctgccgcg aaccagacca tttcgcccaa tgccgggacg tggaacggga
     2281 tcgacgccgc caacgtcgcc cgcatgcgcc tgcagaaccg cttcctgacc ggcatcgaca
     2341 tcgcccgcta caccgcgaag atcatgcgcg ccgacatggc tgcctatgat gccgatccgg
     2401 cgaactacac gcagtcgctc ggctgctggc acggcttcat cggccagcag aagatgatct
     2461 ccatcaagaa gcacttcggc acgaccaagg gccgctacct gtacctctcg ggctggatgg
     2521 ttgccgcgct gcgcagcgag ttcggcccgc tgcccgacca gtcgatgcac gagaagacct
     2581 cggtcccgat gctgatcgaa gagctctaca ccttcctcaa gcaggccgac gcccgcgagc
     2641 tgggcggcct gttccgcgag ctcgacgccg ccaaggccgc cggtgacgcg gtcggcatcc
     2701 accgcacgct cggccagatc gacgccttcc agacccacgt cgtgccgatc gtcgccgaca
     2761 tcgacgccgg cttcggcaat gccgaggcga cctacctcct cgccaagaag ttcatcgagg
     2821 cgggcgcctg ctgcatccag atcgagaacc aggtctccga cgagaagcag tgcggccacc
     2881 aggacggcaa ggtcaccgtt ccgcacgagg acttcatcgc gaagatccgc gccgtccgct
     2941 atgccttcat ggaactgggc gtcgacgatg gcgtgatcgt cgcccgcacc gactcgctcg
     3001 gcgccggcct gaccaagcag atcgccttcg tccgcgaagc cggcgacatc ggtgaccagt
     3061 acaacgcgtt cctcgattgc gaag
//
LOCUS       DATNBV010000292         3088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_185291,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000292 DATNBV010000000
VERSION     DATNBV010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185291"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VN627_07050"
     CDS             <1..297
                     /locus_tag="VN627_07050"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXD03049.1"
                     /translation="SDSGYKTRPFDRILAELRGFFAVHRAEGTHAGGIHIEMTGKDVT
                     ECTGGAVAITDEALADRYHTHCDPRLNAAQSLELAFLLAEELNREAAVRTKAAA"
     gene            complement(398..670)
                     /locus_tag="VN627_07055"
     CDS             complement(398..670)
                     /locus_tag="VN627_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HXD03050.1"
                     /translation="MTSKLFGRASLAAAVAALALTANPAEARTCKRLNKTEGAAVGAV
                     AGGVLGAVLTGGTTAGVLGGAAVGGIGGHEIARTKYNRHCRRYYRR"
     gene            complement(867..1136)
                     /locus_tag="VN627_07060"
     CDS             complement(867..1136)
                     /locus_tag="VN627_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03051.1"
                     /translation="MNQKDSNRHQQGQQQNRQAGQQQNQDGLRQPQQGQSSSKGQMGQ
                     QGGKQQQGGSPTSGQQQQQSDQQNRQSGQKSQQGSRSGSSERRDR"
     gene            complement(1219..1884)
                     /locus_tag="VN627_07065"
     CDS             complement(1219..1884)
                     /locus_tag="VN627_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03052.1"
                     /translation="MTRSLRIALPCKSARPSRRTSTAAFAGEEPAADDPLLAFRPFMH
                     PVPRRNSITPARQRAFIAALAASGIVTQAARKIGASLEALYKLRHRTGAEGFAAAWDA
                     AIDRGMARLEDCALERAIMGEERPIVKHGKVVATWRRYDTALLMFLLRQRRQARYSPL
                     TTIQPGDPVYERLKAMWQAEEFVSAEEVRASLDAKLARLREVVLESQGHIEDQAGEER
                     GGA"
     gene            2089..2379
                     /locus_tag="VN627_07070"
     CDS             2089..2379
                     /locus_tag="VN627_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03053.1"
                     /translation="MKKTAIFAAATMVLALSACGKKDANEAASASTEAAGAAAEASDA
                     AADAAAAASGAAMSSGDAAAAAADQAAAAGKKAEEAGDKAEKAGDKAKDAAH"
     gene            complement(2567..>3088)
                     /locus_tag="VN627_07075"
     CDS             complement(2567..>3088)
                     /locus_tag="VN627_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXD03054.1"
                     /translation="RVPPCARSGPAGFASPGLPNPSPLAFAKAICQLRHRRSDYFSDC
                     SFGEPAWDMLLDLYVGHHEGKVARTTGLCAASLTPTSTGLRYIALLERLDLARREADG
                     GDQRVTNVTLSAKGLAMMDEFLTTMLARFTSTLFEGCRATLHASFDEPSPPAQAQQGL
                     ASQRAGNGDLPLR"
BASE COUNT          486 a         1090 c         1008 g          504 t
ORIGIN      
        1 tcggactcgg gctacaagac ccgcccgttc gaccggatcc tcgccgagct ccgcggcttc
       61 ttcgccgtcc accgggccga gggcacccat gccggcggca tccacatcga gatgaccggc
      121 aaggacgtga ccgaatgcac cggcggcgcc gtcgccatca ccgacgaggc cctggccgat
      181 cgctaccaca cccactgcga cccgcgcctc aacgcggccc agtcgctcga gctggccttc
      241 ctgctggccg aagagctgaa ccgggaagcg gcggtgcgga ccaaggcagc ggcgtgattt
      301 gatagtgtgc ccctgacatc cgtcaggtgc cgcaagggac gcaccaagcc caatcaaaaa
      361 acccccggat cgctccgggg gttttcattt taagggctta ccgccgataa tagcgccggc
      421 agtgcctgtt gtacttggtc cgggcgatct cgtgcccgcc gatgccgccc acggccgcac
      481 cgccgagcac gccggcggtc gtcccgccgg tcagcaccgc gccgagcacg ccgccagcca
      541 cggcgccgac agcagcgccc tcggtcttgt tgagccgctt gcaggtgcgc gcttcggccg
      601 ggttggcggt cagcgccagg gcggccacgg cggccgccag gctggcgcgg ccgaacagtt
      661 tgctggtcat ggggtggtgt cctcctcaga tcggtttgct tgcccctcga aacgctgatt
      721 gcccgatccg gtgcccgtag caagcggttt gcgttggcgg ggcgcgccgc cgacattcgt
      781 cggtgcgcaa gggggcggct ttcccgcacc cgacaaacaa acggcgcctc cccgaaggga
      841 agcgccgctg gtttcataac gaaaggttag cggtcgcgcc gctcgctcga tccgctcctg
      901 gagccctgct ggctcttctg gccggattgc ctgttctgct ggtcagactg ctgctgctgc
      961 tgtccgctcg tcggagagcc gccctgctgc tgcttgccac cctgctggcc catctggccc
     1021 ttcgaggagg attggccctg ctgcggctgg cgcaggccgt cctggttctg ctgctggccg
     1081 gcctggcgat tttgctgttg gccctgctgg tgacggttgc tgtccttctg gttcatggtc
     1141 tctctccggg tcctgacgcg ccggaggccc ttgcctcccg cacgtccccc ttcaaacggt
     1201 ccgatgcgcc cggacgtttc acgctccgcc gcgttcctcc cccgcctgat cctcgatatg
     1261 tccctggctc tccagcacca cctcgcgcag ccgggccagc ttggcgtcga gcgaggcgcg
     1321 cacttcctcg gcgctgacga attcctccgc ctgccacatc gccttcaagc gctcatagac
     1381 cggatcgccc ggctggatcg tggtgagcgg gctgtagcgg gcctgacgcc gctggcgcag
     1441 caggaacatc agcagggcgg tatcataacg ccgccaggtc gccacgacct tgccgtgctt
     1501 cacgatcggg cgctcctcgc ccatgattgc ccgttcgagc gcgcaatcct ccagccgggc
     1561 catgccccgg tcgatcgccg catcccaggc cgcggcaaag ccttccgcgc cggtgcggtg
     1621 gcgcagcttg tagagcgcct caaggctggc cccgatcttc cgcgccgcct gggtgacgat
     1681 gccgctcgcc gccagcgccg cgatgaaggc gcgctggcga gcgggagtga tcgaattgcg
     1741 gcgcggaaca ggatgcatga acggccggaa ggcgagcagc ggatcgtcgg ctgcgggttc
     1801 ctcgccggca aaggcggcgg tcgaggtgcg ccgggaaggg cgggcagact tgcagggcag
     1861 ggcgatgcga agcgaacggg tcatagtggg tactcatctg gatgagcatc acagttaacc
     1921 tattcggtta taagtaggaa agtccgcgct catccggggc cttggcagcc tgccccattc
     1981 cggccacaaa tgcgacgaaa tgatgccaca tttcaatgaa ttaacttgca cggcgcgatt
     2041 cccccggcat cttgccgcca cccagaacca caacgtaggg gaatgcccat gaagaagacc
     2101 gccattttcg ccgccgccac catggtgctc gcgctctcgg cctgcggcaa gaaggatgcg
     2161 aacgaggccg cctcggccag caccgaagcc gccggcgctg ccgccgaggc cagcgatgcc
     2221 gccgctgacg ccgccgccgc cgcctcgggc gccgcgatga gctcgggcga tgccgccgcc
     2281 gccgccgccg accaggccgc cgctgccggc aagaaggccg aggaagccgg cgacaaggcc
     2341 gaaaaggccg gcgacaaggc caaggacgcc gcccactaag cctagaggct gacccgccgc
     2401 acgcaccgcg gcggggtttc ggaagagccg ggcggaggac cacgcccggc tctttcctgt
     2461 ctcatttgat cgttttcccc gcgcgccaca ctcctccccc cggttcgcgg agccgctcct
     2521 cccccgctac cttcctcggc gggggaggag ccttgggagg cagcattcaa cgaagcggca
     2581 gatcgccatt tccggcacgc tggcttgcaa ggccttgctg agcctgcgcc gggggcgacg
     2641 gctcatcgaa gctggcatgc agcgttgcgc ggcacccttc gaacagggtg ctggtgaacc
     2701 gggccagcat cgtggtcaga aattcatcca tcatggcaag gcccttcgcc gacagcgtca
     2761 cgttcgttac ccgctgatcc ccgccgtcgg cctcgcgcct ggccagatcc agccgttcga
     2821 gcagggcgat gtagcgcagc cccgtgctgg tcggcgtcag agacgcggcg cacaggccgg
     2881 tcgttcgggc gaccttgccc tcatggtggc cgacatagag atcgagcagc atgtcccatg
     2941 cgggctcgcc aaacgaacaa tcgctgaaat aatccgatcg cctgtgacgc aactggcata
     3001 tggctttggc gaaggccagc ggcgaagggt tcggcaggcc gggtgaagcg aacccggccg
     3061 gcccggaccg ggcacatggc ggaacccg
//
LOCUS       DATNBV010000293         2325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_816559,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000293 DATNBV010000000
VERSION     DATNBV010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816559"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1010
                     /locus_tag="VN627_07080"
     CDS             108..1010
                     /locus_tag="VN627_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD03055.1"
                     /translation="MADRPSWHLEKMMLIENRVAVITGGASGIGRASSVRFLEEGASV
                     VVADVNPASGEAFLKLAEDLGFADRVRFVTTDVTNEADVERMIGTAVDAFGKLDCMFN
                     NAGIPGALGPLEEVAVEDWDFTFAVLVKGVFLGLKHAVRQFKAQATGGVILNTSSASG
                     LVGGAGPRPYSVAKAAVAHLTTVAAVDVAPYRIRVNAIAPGAILTEIGGQDPVAAAER
                     LSKGQPWPDHGMPSDIASTAVFLASDEARFITGQTIVVDGGATSDSGMEARLQRAGSW
                     ATITGMHHGTTGKKATLRRLDRQN"
     gene            1087..>2325
                     /locus_tag="VN627_07085"
     CDS             1087..>2325
                     /locus_tag="VN627_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXD03056.1"
                     /translation="MNLDLSEENNLLVSMVEKLLGGAGGVDRLRKLDPGTVDRDLYVQ
                     LAEMGILTMRALPTEQGGSALFDTMLAMEQAGRHLPRLPLAETIVAASLLGRSPGDAA
                     AAWLARIGSGDAVVTLALHDRAARPDQLVAFGGACDAVIYRQGDGLYLQEGPLAPVRA
                     DHADQALGRLGATGKPVIELAQGEPAVTLFSAAVEEWKLLISAELWGISAALLEMASR
                     HANERHQFDRPIGSFQAISHPLADRAVDNEAARLLGWWTSWLLAEGSSEACAAISMTF
                     AWSVRTADATARRALHTFGGYGLSLEHNAQVFFRHAKALPLLLGDPSDEMIRVADRMW
                     RGETAALPDGGPCPLSFGQDRETLATVADTRAILEPLMTPDLRASLPDSYDGHVPQVA
                     QALGRAGLLFSDWPKEWGGRG"
BASE COUNT          393 a          760 c          780 g          392 t
ORIGIN      
        1 atcgaatatt tctggcagtc caacatcgcc gcatcactgt tgcgctgctg cgaacggtcc
       61 gccgatgggt cagttgattt tcgcgaacgc gagcggacac agtgacgatg gccgaccggc
      121 cgtcgtggca tttggagaaa atgatgctga tagagaatag ggtcgctgtc atcaccggcg
      181 gtgcaagcgg tattggccgg gccagcagcg tgcgcttcct tgaagaaggc gccagcgtcg
      241 tggttgccga tgtcaatccg gcgagcggcg aggcctttct caagctggcc gaggatctgg
      301 gatttgccga tcgcgtgcgc ttcgtgacga cggacgtcac gaacgaggcc gacgtggaac
      361 gcatgatcgg aacggcggta gacgccttcg ggaagctcga ctgcatgttc aacaatgccg
      421 gtattcccgg agccttggga ccgctcgagg aagtcgcggt cgaggactgg gatttcacgt
      481 tcgcggtgct ggtgaagggc gttttcctcg ggctcaagca tgcggtccgc cagttcaagg
      541 cccaggcgac gggcggtgtc atactcaaca cgtcgtcggc atcagggctg gtcggcggcg
      601 caggcccccg cccctattcc gtggccaagg ccgccgtggc ccacctcacc acggtcgcag
      661 cggtcgatgt cgcgccctac cgtattcggg tgaacgccat cgcgcccggg gcaatcctca
      721 cggaaatcgg cggtcaggac cccgtagctg cggccgagcg gctgtccaag ggccagccct
      781 ggcctgacca cggcatgccg tcagacatcg cctcgacggc ggttttcctg gccagcgacg
      841 aagcgcgctt catcaccggc cagacgatcg tggtcgatgg cggggcgacc agcgacagcg
      901 gcatggaagc acgcctgcag cgcgcaggct cctgggccac gattacggga atgcatcacg
      961 gcaccactgg aaagaaagcc acgttgcgcc ggctcgaccg tcagaactga ccttcatctc
     1021 ctgccgaagc cgatcgacga ggcgagtgcg gcaggcagtg cggaacggga ccatggagaa
     1081 ctcggcgtga atctcgatct gagtgaagaa aacaacctgc ttgtctcgat ggtcgaaaag
     1141 ctgctcggcg gggcgggcgg tgtcgatcgc ttgcgcaagc tggatccggg cacggtggac
     1201 cgcgacctgt atgtccagct tgccgagatg ggcatactga cgatgcgggc gctgccgacc
     1261 gaacagggcg gctcagctct cttcgacacg atgctcgcga tggaacaggc cggtcgccac
     1321 ttgccgcgcc tgccgctggc cgaaacgatc gtggccgcgt cgcttctagg caggtcgccg
     1381 ggtgacgccg ccgctgcgtg gcttgcgcgg ataggcagcg gcgatgccgt tgtcaccctg
     1441 gcgctccatg accgcgcagc ccggcctgac caactggttg cctttggcgg cgcgtgcgac
     1501 gccgtgatct accggcaagg cgatggattg taccttcagg agggcccgct cgcgccagtc
     1561 cgtgccgatc acgcggacca ggccctcggc cgactgggcg ccaccggcaa gcccgtcatc
     1621 gagttggcgc agggcgaacc ggctgtcacc ctcttcagcg cagctgtgga ggaatggaag
     1681 ctcctgatct ccgccgagct atgggggatc tctgccgccc tgctcgagat ggcttcgcgc
     1741 cacgccaatg aacggcacca gttcgatcgc cccatcggca gcttccaggc catctcgcat
     1801 cccctggcgg accgcgcggt ggataacgag gctgcgcgcc tgctcggctg gtggacctcc
     1861 tggttgctgg ccgagggaag cagcgaagcc tgcgccgcca tcagcatgac cttcgcgtgg
     1921 agcgtcagga cggctgacgc aacggcccgg cgtgccctgc acaccttcgg cggctatggc
     1981 ctgtcgctgg aacacaatgc ccaggtcttt ttccggcacg ccaaggcgct gcccttgctg
     2041 ctgggcgacc ccagcgacga aatgatccgc gtggccgacc gtatgtggcg aggggaaacg
     2101 gccgcccttc ccgatggcgg cccctgcccg ctgtcgttcg gacaggaccg ggaaaccctc
     2161 gccaccgtcg cggatacgcg cgccatcctc gaacccctga tgacccccga tctgcgggcc
     2221 agcctgcccg attcctacga cggtcacgtc ccgcaagtcg cgcaggcgct gggccgtgcg
     2281 ggactgctct tctccgactg gcccaaggaa tggggcggcc gcggc
//
LOCUS       DATNBV010000294         3606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2730304,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000294 DATNBV010000000
VERSION     DATNBV010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2730304"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VN627_07090"
     CDS             <1..507
                     /locus_tag="VN627_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HXD03057.1"
                     /translation="RTVEDARALLLAGADKVAVNSAAVSRPEVVSDIAEKMGSQCIVA
                     SVDARRVGDHWEIFTHGGRKATGIDAVEHAVKLARLGAGELLVTSMDGDGTQAGYDLE
                     LTRTIADAVSVPVIASGGVGTLDHLVDGVTKGHASAVLAASIFHFGKHSIAEAHAALR
                     AAGLPARG"
     gene            638..958
                     /locus_tag="VN627_07095"
     CDS             638..958
                     /locus_tag="VN627_07095"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HXD03058.1"
                     /translation="MSETLANLEATIAQRRAADPSTSYVAKLNAKGVGKIAQKVGEEA
                     TETVIAALSGDRAELVGEAADLLFHLLVLLGAKGVPLAEVLAELDRREGVSGIAEKAS
                     RKAD"
     gene            958..1335
                     /locus_tag="VN627_07100"
     CDS             958..1335
                     /locus_tag="VN627_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HXD03059.1"
                     /translation="MPIDPRAPYDDNNIFAKILRGEIPSRKVYEDDFAYAFHDINPQA
                     PVHLLVIPKGRWVSWDDFSAEASEAEIAGFARAVGHVAREHGLVEPGYRMLANVGQHG
                     HQEVPHLHVHLFGGRQFYAMIPQ"
     gene            1556..2008
                     /locus_tag="VN627_07105"
     CDS             1556..2008
                     /locus_tag="VN627_07105"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HXD03060.1"
                     /translation="MTVPVPPAGLFEGAVHRFAVRAYYEDTDLSGVVYHANYLRWFER
                     ARSDLVRLLGIDQRAAQEGGEGTYAVSELAIRYLAPARLDDVVIVETLAQEAGAASCR
                     LVQRALREGLLLADAQVRVGFIGPDGRPRRQPADWRAAFQRILMTETP"
     gene            2005..2706
                     /gene="tolQ"
                     /locus_tag="VN627_07110"
     CDS             2005..2706
                     /gene="tolQ"
                     /locus_tag="VN627_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HXD03061.1"
                     /translation="MILNLLASGAAASAHLNPVKLFLDADIVVQLVMLGLLLASVWVW
                     TIVVSFSVRLGAVNRADAAYEQGFWQARDIEAYHQLNNRSDAPTARVAAAGLAERGRN
                     LGAKGLDLEGARQRLAASMGAVVEQESDALGSRLNFLATVGSAAPFIGLFGTVWGIMN
                     SFFQIGQQQNSSLAVVAPGISEALFATAIGLFAAIPAVIAYNRFSNAVNHFEARLQRF
                     ADRFHTTLSRELEAE"
     gene            2861..3313
                     /locus_tag="VN627_07115"
     CDS             2861..3313
                     /locus_tag="VN627_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXD03062.1"
                     /translation="MAMNLSSSGSRRRGRRGKRGAMAEINVTPLVDVMLVLLIIFMVT
                     APLLTAGVPVELPDSRANALPQEPKQVTISMTSDGKTYLDQAEVAPGELGQRLAGIAP
                     GADGKPPLVTLRADKALPYGDVMAVMGELNHAGFNSISLVTNGSVGAP"
     gene            3321..>3606
                     /locus_tag="VN627_07120"
     CDS             3321..>3606
                     /locus_tag="VN627_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03063.1"
                     /translation="MMASLSAYREHGLRPEEKAGLVVALAAHAALIAWLMLNPPAPKP
                     VPMPERMTVTIADEVAERSTSPEPKAEAAPATAPVLAEQPAPAPVPSPVIQ"
BASE COUNT          533 a         1103 c         1358 g          612 t
ORIGIN      
        1 cgcacggtgg aggatgcgcg ggcgctgctg ttggcgggcg cggacaaggt ggcggtgaat
       61 tccgcggcgg tttcgcggcc cgaggtggtg agcgacattg ccgagaagat gggttcgcag
      121 tgcatcgtgg cctcggtcga cgcgcgccgg gtgggcgatc actgggagat atttacccac
      181 ggcgggcgca aggcgaccgg gatcgacgcg gtcgagcatg cggtgaagct ggcgcggctg
      241 ggggcgggcg agctgctcgt cacctcgatg gacggggacg gcacgcaggc cggctatgac
      301 ctcgaactca cgcggacgat tgccgatgcg gtttccgtgc cggtcatcgc gagcgggggc
      361 gtgggcacgc tcgatcatct ggttgacggc gtcaccaagg gccatgccag cgcggtgctg
      421 gcagcgtcca tcttccactt cggcaagcac tcgatcgccg aggcccatgc ggccttgcgc
      481 gcggcagggc tgcccgcgcg cggatgattc ccgcttgcgt gtgcgccgta tcggcgacgt
      541 tgcggtggcg gtgaggtgat cccatgggtc ctgaaacagg ttcaggacga cggcaggtgg
      601 ggttgaccct ttcttcgcgc ggcggcatcg ctcgggcatg agcgagaccc tggcaaatct
      661 tgaagcgacc attgcgcagc ggcgcgcggc cgatccgtcc acgtcttatg tggccaagct
      721 caatgcgaaa ggcgtgggca agattgcgca gaaggttggc gaggaggcca ccgagacggt
      781 gatcgccgcg ctctccggcg accgggccga gctggtcggc gaggcggcgg acctgctgtt
      841 tcacctgctg gtgctgctgg gagcgaaggg cgtgccgctg gccgaggtgc tggccgagct
      901 ggaccggcgc gagggtgttt cggggattgc cgagaaagct tcaaggaagg ctgactgatg
      961 ccgatcgatc cccgggcgcc ctatgacgac aacaacatct tcgcgaagat cctgcgcggg
     1021 gagatcccct ctcgcaaggt ttacgaggac gacttcgcct acgccttcca cgacatcaac
     1081 ccgcaggcgc cggtccacct gctggtgatc cccaaggggc ggtgggtgag ctgggacgac
     1141 ttttcggccg aggcaagcga ggcggagatc gccgggttcg cgcgggcggt gggccatgtg
     1201 gcgcgggagc acgggctggt tgagccgggt taccggatgc tggcgaacgt gggccagcac
     1261 gggcaccagg aagtgccgca cctgcatgtg catttgtttg gcgggcggca gttctacgcc
     1321 atgatcccgc agtaggtttg cgtcctcccg ggcttgaccc gggaccggtg tgggtggcgg
     1381 aggtgcggcc ttgcgggcag gctggccttt cagcttcgcg ttgggcagcg gtcccggatc
     1441 aagtccggga cgacggggca ggcgagtcca gcggagccgg gggcgcggcg ggtcgagaac
     1501 atttccggtc atcgcccttg ccctgcgact cgaccattgg ttaataccgc agccgatgac
     1561 cgtccccgtc ccaccggcag gcctgtttga aggcgcggtg catcgcttcg cggtgcgggc
     1621 ctattacgag gacaccgatc tttcgggcgt ggtctaccat gccaactacc tcaggtggtt
     1681 cgagcgggcg cggtcggacc tcgtgcggct gctggggatc gaccagcgtg cggcgcagga
     1741 gggcggcgag gggacatatg cggtatccga gttggccatc cgctatctgg ccccggcgcg
     1801 gctcgatgac gtggtgattg tcgaaaccct ggcgcaggag gcgggcgcgg cttcgtgccg
     1861 gctggtccag cgggccttgc gcgaggggct gctgctggcc gatgcgcagg ttcgggtggg
     1921 gttcatcggg cctgatggaa ggccccggcg ccagcccgcc gactggcgcg ccgcttttca
     1981 acgcattctc atgacggaaa ctccatgatc ctgaacctgc tagcgagcgg cgcggccgca
     2041 tcggcccatc tcaatccggt gaagctgttc ctcgatgccg atatcgtggt gcagctggtc
     2101 atgctgggcc tgctgctggc gagcgtgtgg gtgtggacca tcgtggtctc cttctcggtg
     2161 cggctgggcg cggtgaaccg ggccgatgcc gcttatgagc agggcttctg gcaggcgcgc
     2221 gacatcgagg cctatcacca gctgaacaac cgcagcgatg cgccgaccgc gcgggtggcg
     2281 gcagccggtc tggccgagcg cggacgcaac ctcggcgcca aggggctgga cctggagggt
     2341 gcgcggcagc ggctggccgc cagcatgggc gcggtggtcg agcaggagag cgatgctctg
     2401 ggcagccggc tcaatttcct ggcgacggtg ggttcggcgg cgccgtttat cggcctgttc
     2461 gggaccgtct gggggatcat gaactcgttc ttccagatcg ggcagcagca gaattcctcg
     2521 ctggcggtgg tggcgccggg catttccgag gcgctgttcg cgacggctat cggcctgttc
     2581 gcggcgatcc cggcggtgat tgcctacaac cgcttttcca atgcggtgaa ccacttcgag
     2641 gcgcggcttc agcgcttcgc cgaccgtttt cacacgacgc tgagccgcga actggaggct
     2701 gagtgatgat aggcgcgggt atcctcccca ctcgtgggga ggtggcagcc gcgcagcggc
     2761 tgacggaggg gggcatcggg accgcgcggc ggtgcgtggt ggagagcccc ctccaccgcc
     2821 tgcggcggtt cccctcccca cgagtgggga ggatctaaca gtggcgatga acctctcatc
     2881 gtcgggctcg cgccgccgtg ggcggcgcgg caagcgcggg gccatggcgg agatcaacgt
     2941 caccccgctg gtcgacgtca tgctggtgct gctgatcatc ttcatggtga ccgcgccgct
     3001 gctgaccgcc ggcgtaccgg tggagctgcc cgacagccgg gccaacgcct tgccgcagga
     3061 gcccaagcag gtgaccatct cgatgacgag cgacggcaag acttatctcg accaggccga
     3121 ggtggcgccg ggcgagctgg gccagcggct ggcgggaatc gcgccggggg ctgacggcaa
     3181 gccgccactg gtcaccctgc gggcggacaa ggccctgcct tacggcgatg tcatggcggt
     3241 gatgggggag ttgaaccacg cggggttcaa ttccatttca ctggtcacca acggttcagt
     3301 tggcgcacca tagctcgggg atgatggcaa gtctcagcgc atatcgggag catggtctcc
     3361 ggcccgagga gaaggcgggg ctggtggtgg cgctggcggc ccatgccgcg ctgattgcct
     3421 ggctgatgct gaacccaccc gcgcccaagc ccgtgcccat gcccgagcgc atgacggtga
     3481 cgattgccga cgaagtggcc gagcgctcga cctcgcccga gccgaaggcc gaggcggcgc
     3541 cggcgactgc cccggtgctg gccgagcagc ctgcgccggc accggtgccc tcgccggtga
     3601 tccagc
//
LOCUS       DATNBV010000295         2782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3664448,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000295 DATNBV010000000
VERSION     DATNBV010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3664448"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VN627_07125"
     CDS             complement(<1..741)
                     /locus_tag="VN627_07125"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXD03064.1"
                     /translation="MELKWLEDFLSLSETRNFSRSAKLRFTTQPAFSRRIKALEEWVG
                     ATLFDRSAQPVSLTEAGIAFRSLAEEVVRQLYQGREEIHLASVHVASAIKVAATHSLS
                     LTFFPQWIRSIESAAGILNIRLDSGHAGACVQMMQHGACHFMLCHTHPSVEVGLDPRQ
                     FLARRVGTDRLLPVSRPDAAGRPIDSLDGGSNGLCRYLAYAETSAIGRAVEHMLRQST
                     RHVAMDKVFVSRLAAVLKSMVLDGRGFAW"
     gene            892..1860
                     /locus_tag="VN627_07130"
     CDS             892..1860
                     /locus_tag="VN627_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HXD03065.1"
                     /translation="MLLVPEQLARELVSTEDALSAVGAAFAAAHAGRARSYPVVRELV
                     GHADAVFGVKAGFDAGLPVLGLKAGGYWPGNAAQGRGNHQSVVLLFDPDSGQAVALVG
                     ANYLTGVRTGAASALAARHLARPEAAVLGLIGTGGQSLFQFRAALGVRPITRVLAWDP
                     NEASLDAFGKAVSAAGLAFEAPGLEAVARQADILITVTPSRAALIPREWIKPGTHISA
                     MGADTAGKQELAPELIAAATLVVDDAEQAITIGECQHAYRLGLITRDRLGLTLGAVVS
                     GAFARASAEEITVFDGTGVALQDLAVAELAYRRARERGLGVDCDMR"
     gene            1942..>2782
                     /locus_tag="VN627_07135"
     CDS             1942..>2782
                     /locus_tag="VN627_07135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HXD03066.1"
                     /translation="MAKAQDWLKATPGGLVCEPGGFTIDPTAPVERAVITHGHGDHAR
                     PGSTHVLATSDTLEIMRTRFGPEAGASLQPLDYGDWLELGGVNLAFAPAGHVLGSAQV
                     VMEYAGQRVVVSGDYKRRPDPTCVAFEPVGCDLFITEATFGLPVFRHPDDAGEIAKLL
                     ESLRLFPERTHLVGAYALGKAQRVMALLRRAGWERPIWLHGALFPLTDLYRRLGQDFG
                     ELKPATGAAKAELKGEIVLAPPGALREAWSRRMAEPMRAVASGWMGVRARARQAGAEL
                     PLVI"
BASE COUNT          412 a          955 c          974 g          441 t
ORIGIN      
        1 ccaggcgaag ccgcggccgt ccagcaccat cgatttgagg accgccgcca gccgcgacac
       61 gaagaccttg tccatggcga catgccgcgt ggattgccgc agcatatgtt cgaccgcgcg
      121 gccgatcgcc gaggtctcgg cataggccag ataacggcag aggccgttcg agccgccgtc
      181 gagggaatcg atcggccgcc cggccgcatc ggggcgcgac accggcagca agcggtcggt
      241 tcccacccgc cgcgcgagga attgccgggg atccagaccc acctcgaccg agggatgggt
      301 gtggcacagc atgaaatggc aggcgccgtg ctgcatcatc tgcacgcagg cgccggcatg
      361 gccgctgtcc agccggatat tgaggatccc cgcggcgctc tcgatggatc gaatccattg
      421 gggaaagaag gtgagggaca ggctatgggt ggcggcgacc ttgatggcgc ttgccacatg
      481 gacggaggcg aggtgtatct cctcgcgtcc ctggtagagc tggcgaacca cctcctcggc
      541 caggctgcga aaggcgatgc cggcttccgt cagcgaaacg ggctgggccg aacggtcgaa
      601 gagggtcgcg cccacccact cctcgagcgc cttgatgcgc cggctgaagg cgggctgcgt
      661 cgtgaaccga agcttcgcgg atcgcgagaa attgcgcgtc tcggagaggc tcaggaaatc
      721 ctcgagccat ttcagttcca tgaaggattg ttgcaggcaa attgcagcgc aacaagaccc
      781 tgcgatgccc ccatgcccgg ccggcatgat cgaaggccca agcggcattg gcgccggggc
      841 cctcgatcgg ggtagttaag ctcgctcacc cagccatctg cgagatcgcc catgcttctc
      901 gtccctgaac aactcgcccg cgagctcgtc tcgaccgagg acgccctgtc cgccgtcggc
      961 gccgccttcg ccgccgccca tgccggacgc gcccggtcct atccggtggt gcgcgagctg
     1021 gtcggccatg ccgatgcggt cttcggcgtc aaggcaggct tcgacgccgg cctgccggtg
     1081 ctggggctga aggccggcgg ctattggccg ggcaatgccg cccagggccg gggcaatcat
     1141 caatccgtcg tcctgctctt cgatccggac agcggccagg ccgtggcgct ggtgggggcc
     1201 aattatctga cgggggtgcg gacgggcgcg gccagtgccc ttgccgcgcg ccatcttgcc
     1261 cggccggagg ccgcggtcct cgggctgatc gggaccggcg ggcaatcgct cttccagttt
     1321 cgcgccgcgc tcggcgtgcg ccctatcacc cgcgttctgg cctgggatcc gaacgaggcg
     1381 agcctggatg ccttcggcaa ggcggtatcg gcagcgggcc tcgccttcga ggcgcccggt
     1441 ctcgaagccg tcgcccggca ggccgacatc ctgatcaccg tcaccccgtc gcgggcggcc
     1501 ctcatcccca gggaatggat caagcctggc acccatatca gcgccatggg cgcggatacc
     1561 gccggcaagc aggagctggc gcccgagctc atcgccgcgg ccacgctggt ggtggatgat
     1621 gcggagcagg ccatcaccat cggcgagtgc cagcatgcct atcgcctggg gctgatcacc
     1681 cgcgatcgtc tcggcctgac cctgggcgcc gtcgtcagcg gcgccttcgc gcgggcctcg
     1741 gcggaggaga tcacggtctt cgacggaacg ggcgtggcgc tccaggatct ggccgtggcg
     1801 gagctggcct atcgccgggc ccgggagcgc ggcttgggcg tcgactgcga catgcggtga
     1861 ggaaccctcg cggcgattga gccttttttc tggctagggt gtccgctggc aaagggcgcg
     1921 ggccgagcct atctaggcgc aatggcaaag gcacaggact ggctgaaggc gacgccgggc
     1981 gggctggtct gcgagcccgg cggcttcacc atcgatccca ccgcgccggt ggagcgcgcg
     2041 gtgattaccc atggccatgg cgaccatgcc cggccgggca gcacccatgt gctcgccacg
     2101 tcagacaccc tcgagatcat gcgcacccgc ttcgggccgg aagcgggcgc cagcctgcag
     2161 cccctggact acggcgactg gctcgagctc ggcggggtca atctcgcctt cgccccggcc
     2221 ggccatgtcc tgggctcggc ccaggtggtg atggaatatg ccggccagcg ggtcgtcgtc
     2281 tcgggcgatt acaagcgccg gccggatccc acctgcgtgg ccttcgagcc ggtgggctgc
     2341 gatcttttca tcaccgaggc gaccttcggc ctgccggtct tccgccatcc cgatgatgcg
     2401 ggcgagatcg ccaagctgct ggagtcgttg cgcctctttc ccgagcgcac ccatctcgtc
     2461 ggcgcctatg ccctgggcaa ggcgcaacgc gtgatggccc tgctgcgccg ggcaggttgg
     2521 gagcggccga tctggctcca tggcgcactc ttccccttga ccgatctcta ccgccgcctg
     2581 ggccaggatt tcggcgagct caagcccgcg acgggcgccg ccaaggccga gctcaagggc
     2641 gagatcgtcc tggccccacc gggtgcgctg cgcgaggcct ggtcgcggcg catggccgag
     2701 cccatgcgcg ccgtcgcctc gggctggatg ggcgttcggg cccgggcgcg ccaggccgga
     2761 gccgagctgc cgctggtcat ct
//
LOCUS       DATNBV010000296         7861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3373671,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000296 DATNBV010000000
VERSION     DATNBV010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3373671"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VN627_07140"
     CDS             <1..533
                     /locus_tag="VN627_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HXD03067.1"
                     /translation="SFGLVLIAGIDFPIQFVRRFLRLRMSLQEVRDEHKESEGSPERK
                     AAIRGRQRQLAMGSLQKAMREAQFVITNPTHFSVALIYDPDRAGAPIVLAKGRGEKAR
                     ELEVPLLEYPALARSVYFTTRENQVIREELYAAVAGVLGFVMALKRGETRPLPQVVVP
                     LELRYDPEGRPDPAAA"
     gene            750..2195
                     /gene="fliD"
                     /locus_tag="VN627_07145"
     CDS             750..2195
                     /gene="fliD"
                     /locus_tag="VN627_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HXD03068.1"
                     /translation="MATSGTSATQSSTASSILTALGGTGQIDWNTLASNLSVAQFAGQ
                     TDRLNTKASQLDKQITDAAGIKSSLLTLATSLGDRVRAGDLSPQPSLSNSLVAGLKLN
                     GAATPKGTYSLEVTQLAKARSFAGPPLASTATPIGAGTLTIRFGTVGASFAEDTGHAP
                     VTLTIPQGATLADVASAINGANAGLSAYVSNTADGPRLVIKGQQGGANGFVIAASEDP
                     LDPGLSRLAWEPATGPASQLLTGAQDAAYKLDGLPMTSPTNKVSGLVAGLDATFTATN
                     VGNPATLSFADNTAAITSAMQDLTSALNELVGMVKTATDPLTGDLANDPGARALKRAL
                     SQLSGTTIMPSAPAGAPRTLADLGLSIQRDGTFALDGARLVKTLAADPTGVAAMFTNG
                     LSGVYGTIDGLNRTMTASGNATSLAASIARYTKAKSQASADLAKLADKQEAFRQQMVT
                     RFATTQTAVSASTSTLTFLKNQIDAWNSKTN"
     gene            2232..2618
                     /locus_tag="VN627_07150"
     CDS             2232..2618
                     /locus_tag="VN627_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HXD03069.1"
                     /translation="MTMIARRTPDAVYRRVDFDARVNGADPMELVGLCYEHLVAALGS
                     ALFAHERKDNAAKSAALTRAVSAVTALQLGVKGTEGVAAALLQFYQAAKNTLLDSVLS
                     FDPVRIGQVRADILEIAGALHGARPQ"
     gene            2748..3350
                     /locus_tag="VN627_07155"
     CDS             2748..3350
                     /locus_tag="VN627_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HXD03070.1"
                     /translation="MLRRSNLLLVDGDLRRRAAISHCLSGSGLHVEPFEDVRELYSRW
                     PSDGVLLVHDSGDAIGALISHMSQTGDWLPIIGFAEEPATRRIVEAVLQGAVDYLVWP
                     FAETQVAESMSLAEDRARSVAGAKLREAVARSRVERLTRREREVLAGVAGGLSNRLIG
                     ERLSISPRTVEIHRANMLNKMGASHTSEAIRIAIEASLVN"
     gene            complement(3380..3871)
                     /locus_tag="VN627_07160"
     CDS             complement(3380..3871)
                     /locus_tag="VN627_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03071.1"
                     /translation="MTKQTEPRSGSGRSAPGKAVAGWRRQLGELALACAGSFPSEEAQ
                     RLRDMHRLLAKAPTSAVLRGAVVPDAERLEQLIAAEASSAAALSLLGSQCGYLLSRGA
                     GGQHLASVVLPGAVEEISASGDTLTLALVGALALALADTDLREPECGAAGREAPGRSA
                     RLN"
     gene            complement(4170..5318)
                     /locus_tag="VN627_07165"
     CDS             complement(4170..5318)
                     /locus_tag="VN627_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXD03072.1"
                     /translation="MKIAQVQPFEAIAISRVAHDLAEQGLDVIHMEFGQPSTSAPAAA
                     IAEAHRVLDAEAMGYWESNALKDRIARYYAEAHGVAVDREQVILTCGASPAFVLALSC
                     LFAPGARVALARPGYVAYRNTLRALYLEPVELACGEAERYQVTAAALAALDPAPDGLI
                     IASPANPTGTIIPADELAAIARVCRERGIRIISDEIYHGLSFGAPAASMLEHMADAFV
                     VNSFSKYYSMPGWRLGWAVVPRDAIAAARARMGNLFLTPPVIAQRAGLVAFDCRAELE
                     EHRETYARNRELMLAALPALGLSHVAPPDGAFYLYADIGHLTADSAAFCTDLLRDTGV
                     CTAPGVDFDPVDGRRFIRFSFAVSTEEVRTAIARMVPWFAARTAAALG"
     gene            5821..6855
                     /locus_tag="VN627_07170"
     CDS             5821..6855
                     /locus_tag="VN627_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXD03073.1"
                     /translation="MTFMKTGESGRRIRNISELAKLAGVSAGTVSRALAGKDLVNRNT
                     RERIQALAREHGFRPNQMASRLRTGHTGVIGVVIPLGHERAQLLSDPFFMTLIGHLAD
                     AVTERGYDLMLSRMIPLDEEDWLERITDSGMLDGVVLIGQSDQFATIERVAADYLPLV
                     AWGTHQPGQRHCSVGTDSFVGGQMAARHLLERGARRLAFFGETTGIEIAQRLEGARAA
                     VAESGTGATLEAFPTRLAVNAMADHVGEHLDVMDRATDGIFAASDAIAMTVLRMLHER
                     GRKVPDEIAVIGYDDLPIAMQTMPRLTTVRQDIAAGAAAMVDSLFRRIGGEDAGPTVL
                     EPRVVVRESA"
     gene            7052..7657
                     /locus_tag="VN627_07175"
     CDS             7052..7657
                     /locus_tag="VN627_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HXD03074.1"
                     /translation="MTDRPLIALLAAGRASRFGADKLAAACAGGPLAGHAVAAVMATG
                     LPALCITAPGARPDWLPADIPAVPNPDAAEGLATSVALAAREAARRGAPSLLLHLADM
                     PCVTPALLLAVAEAGAMGACRYPSGRPGVPARFPADRFAELASLAGDRGAAALLQADP
                     AGVLVDCAADDLIDVDTPEALARAEAVLNLRQRKAGQASSG"
     gene            7682..>7861
                     /locus_tag="VN627_07180"
     CDS             7682..>7861
                     /locus_tag="VN627_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HXD03075.1"
                     /translation="MAHYSGPDIAAVESGFGSPLGNVLRILAYMAVVFVSATLGFMAA
                     GWSVGDAAYMVTLTIF"
BASE COUNT         1244 a         2778 c         2613 g         1226 t
ORIGIN      
        1 tgtccttcgg cctcgtgctc atcgccggga tcgacttccc gatccagttc gtccgccgct
       61 tcctgcgcct caggatgagc ctccaggaag ttcgcgacga gcacaaggag agcgagggca
      121 gccccgaacg caaggccgcc atccgcggcc gccagcgcca gttggccatg ggttcgctgc
      181 agaaggcgat gcgcgaggcg cagttcgtca tcaccaaccc gacccacttc tcggtcgccc
      241 tgatctacga tcccgaccgc gccggtgcgc cgatcgtgct ggccaagggc cggggcgaga
      301 aggcgcggga gcttgaggtg ccgctgctcg aataccccgc gctcgcccgc tcggtctatt
      361 tcaccacccg cgagaaccag gtgatccgcg aggagctcta tgccgccgtg gccggcgtgc
      421 tcggcttcgt catggccctg aagcgcggcg agacccggcc gctgccgcag gtcgtggtgc
      481 cgctggagct gcgctacgat ccggaagggc ggcccgaccc ggcggcggcc tgaccgggcg
      541 aggaatcccc cttgcccgcc gggaagaagt ttgggtgagc ggcgggacgc aggcgcaatc
      601 ggtggcggcg gcggcccctc tcgactgcgg ctagcgggca ggcctgcaag cctgcgcatc
      661 tttccccgac ggcgaagagg gcgacgcccc cccgcacaat tccctaatcg ggcccttaat
      721 ccgaccccgc cgccgccgtc ctttccgtca tggcaacctc cggcacctcg gcgacccagt
      781 ccagcacggc cagttcgatc ctgaccgcgc ttggcgggac gggccagatc gactggaaca
      841 cgctggcgtc caacctttcg gtggcccagt tcgccggaca gaccgaccgc ctgaacacca
      901 aggccagcca gctcgacaag cagatcaccg atgcggcggg catcaagtcc tcgctgctga
      961 cgttggccac ttcgctgggc gaccgcgtgc gcgctggcga tctttcgccc cagccgagcc
     1021 tgagcaacag cctcgtcgcc gggctcaagc tcaacggcgc ggcgacgccg aagggcacct
     1081 attcgctcga ggtcacgcag ctggccaagg cgcggtcgtt tgccggaccg ccattggcca
     1141 gcactgccac acccatcggc gcgggcacgc tcacgatccg cttcggcacg gtcggcgcat
     1201 ccttcgccga ggacaccggc cacgctcccg tgacgctgac cattccccaa ggcgcgaccc
     1261 tggccgacgt ggcgagcgcg atcaacggcg ccaacgccgg tctctcggct tatgtttcca
     1321 acacggcgga cgggccgcgg ctggtgatca agggccagca gggcggggcc aacggcttcg
     1381 tcatcgcggc gagcgaggac ccgctcgacc cggggctctc gcgcctcgcc tgggagccgg
     1441 cgaccggccc ggcatcgcag ctgctgacgg gcgcgcagga tgccgcctac aagctcgacg
     1501 gcctgcccat gacctcgccg accaacaagg tgagcggcct tgtcgccggg cttgacgcga
     1561 ccttcaccgc caccaatgtc ggcaatccgg ccaccttgtc gttcgccgac aacaccgccg
     1621 cgatcaccag cgccatgcag gacctgacca gcgcgctcaa cgagctggtc ggcatggtca
     1681 agaccgccac cgatccgctt accggcgatc ttgccaacga tcctggcgcc cgggcgctca
     1741 agcgggcact gtcgcagctc tcgggcacga cgatcatgcc gagtgcgccg gccggcgccc
     1801 cgcgcacgct ggccgacctt ggcctctcga tccagcgcga cgggaccttc gcgctcgacg
     1861 gcgcgcgcct tgtgaaaacc cttgccgccg atccgaccgg ggtcgccgcc atgttcacca
     1921 atggactgag cggcgtctat ggcacgatcg acggcctcaa ccgcaccatg accgccagcg
     1981 gcaacgccac ctcgctggcc gcctcgatcg cccgctacac caaggccaag agccaggcct
     2041 cggccgatct tgccaagctg gccgacaagc aggaagcctt ccgccagcag atggtcaccc
     2101 gcttcgccac gacccagacc gccgtttccg cctccacctc gacgctgacg ttcctcaaga
     2161 accagatcga cgcgtggaat tccaagacca actgagccag ccccgatcgg ccactggagc
     2221 cctgacccgc catgaccatg atcgcccgcc gcacccccga tgccgtctat cgccgggtcg
     2281 atttcgacgc gcgggttaat ggggccgatc cgatggaact ggtgggcctg tgctacgagc
     2341 atctcgtcgc cgcgctgggt tcggccctgt tcgcccatga gcgcaaggac aatgccgcca
     2401 agagcgcggc cctgacccgt gccgtttcgg cggtcacggc gctgcagctt ggggtcaagg
     2461 gaaccgaggg cgtggccgcg gcgctgctcc agttctacca ggcggcgaag aacaccctgc
     2521 tcgacagcgt cctctcgttc gatccggtgc ggatcgggca ggtccgcgcc gatattctcg
     2581 agatcgcggg cgcgctgcac ggcgcgcggc cccagtgatc ggccccagtg atcgggcctc
     2641 aactattggg acccagcgat cagggcgcag tttgccttgc ggacgatgcc accgaaacgg
     2701 accggtctca gtttttaccc ctacgcgcgt tccgttagga taatcgcatg ttgagacgct
     2761 ccaatctcct cctcgtcgat ggcgatctgc gccgccgggc cgccatttcc cattgccttt
     2821 caggcagcgg ccttcacgtg gagccgttcg aggacgtgcg cgaactctac tcgcgctggc
     2881 cgagcgacgg cgtgctgctg gtccacgata gcggcgatgc catcggcgcg ctgatctccc
     2941 atatgagcca gaccggggac tggctgccga tcatcggctt cgcggaagaa cccgccaccc
     3001 gccggatcgt cgaagccgtg cttcagggcg cggtcgatta tctggtctgg cccttcgcgg
     3061 aaacgcaggt ggcggaatcg atgagccttg cggaagaccg cgcccgctcg gtcgccgggg
     3121 ccaagctgcg cgaagccgtt gcccgcagcc gggtcgaacg cctcacccgg cgggaacgcg
     3181 aagtgctggc gggcgtcgcc gggggcctgt ccaaccgcct gatcggcgaa cggctcagca
     3241 tctcgccgcg cacggtcgaa atccaccgcg ccaacatgct caacaagatg ggtgccagcc
     3301 acacctccga agcgatccgc atcgcgatcg aggcttcgct ggtcaactga ccggcttgcg
     3361 tcgaaccggg gcgggccgct cagttcaatc gggcgctccg gccgggggcc tcccggcccg
     3421 ccgcgccgca ttcgggctca cgcaagtccg tgtcggccag ggccagggcg agcgcaccga
     3481 cgagcgccag ggtcagggta tcgccgctgg cgctgatttc ctcgaccgcg ccgggcagga
     3541 ctaccgaggc caggtgctgg ccgccggccc cgcgcgacag gagatagccg cattggcttc
     3601 cgagcaggga cagggccgct gccgagctgg cttccgcggc gatcagctgc tccagccgct
     3661 cggcatcggg aaccaccgct ccgcgcagca cggccgacgt gggggccttg gcgagcaggc
     3721 gatgcatgtc gcgcaggcgc tgggcttcct cggacgggaa cgagccggcg caggccagcg
     3781 cgagttcccc caactgccgc cgccaaccgg cgactgcctt gccgggagca ctgcggccgg
     3841 aaccactgcg cggttcggtc tgcttcgtca cgattgcgtt tcctccgacc tgcggcctgc
     3901 tggccgcctg tatccgaccc gtggtcttcg cttgcctcaa tcgttggtca atcgcggtga
     3961 actccgcgat tttcctgccg tcccattgtg cgacggagcc tgaagcaggc gtgaacctgc
     4021 agacggcgcc tcagcgccag aacctgcaag cggcgttatg gttcccgata ttgggatgta
     4081 gcggggggtt tgcgggcagg ccgcaagaac gaccgagcgg ttgcagcgtc agccgcgaga
     4141 ttgatcgggt gcggcgcggc gagcccggct caccccagtg ccgccgcggt gcgcgcggcg
     4201 aaccagggca ccatccgcgc tatcgccgtc cgcacttcct cggtggaaac cgcaaagctg
     4261 aaccggatga accgccgccc gtcgaccgga tcgaaatcca ccccgggcgc ggtgcagacc
     4321 ccggtatcgc gcagcaggtc ggtgcagaag gcggcggaat cggcggtgag gtggccgatg
     4381 tcggcataaa ggtagaacgc cccgtccggc ggcgcgacgt ggctgagccc gagcgcgggc
     4441 agcgccgcga gcatcagctc acggttgcgg gcgtaggttt cgcggtgctc ctcgagttcc
     4501 gcccggcagt caaaggcaac gagcccggcg cgctgggcga tcaccggcgg ggtgaggaac
     4561 aggttgccca tccgcgcccg ggcggcggcg atggcatcgc gcggcaccac tgcccagccc
     4621 agccgccagc cgggcatcga gtaatacttc gaaaagctgt tgacgacgaa ggcatcagcc
     4681 atgtgctcaa gcatcgaagc cgccggagcg ccgaaggaga ggccgtgata aatttcatcc
     4741 gaaatgattc taattccgcg ctcccggcag acgcgggcga tggcggccag ttcatcggcc
     4801 gggataatcg tgcccgttgg gttggcgggg ctggcgatga tcaggccgtc cggtgccggg
     4861 tccagcgccg cgagggcagc cgccgttacc tggtagcgct cggcctcgcc gcacgccaat
     4921 tcgaccggct cgagataaag cgcccgcagg gtattgcgat aggcaacata gcccggacgg
     4981 gccagcgcga cccgcgcccc cggagcaaac aggcaggaga gcgcaagcac gaaggccggc
     5041 gaggcgccgc aggtcaggat cacctgttca cggtcaaccg ccacgccatg cgcctcggcg
     5101 tagtagcggg cgatccgatc ctttaatgca ttgctttccc agtagcccat ggcctcggca
     5161 tcgagcaccc ggtgggcctc ggcgatggcc gcggcgggcg cgctggtcga aggctggccg
     5221 aactccatgt ggatcacgtc gagcccctgc tcggccagat cgtgggcgac ccggctgatg
     5281 gcaatggcct cgaacggctg gacctgagcg atcttcatgg ggcggtgcca ggccgtagac
     5341 tcatgaatgg caccatcgcg gcgccggaaa ggcgaacaga tcgggccgag gcgtccgccg
     5401 gaggggctgg ttgccccgcc aagggcgcgt tggttcgagc tggaagccat tccggcgtcc
     5461 ggaggatttg accaaaatag ccatctgcgg cgtcaggaaa gctagagata tcgacatatc
     5521 cctgcgcttt ccttccttgc ccctggccat tttgccgcaa acctcgacgg tgccgttcat
     5581 gagtttacgg cctaggcggc gcggaccaat tccgcaacgc cggattgccg ggttgcggac
     5641 aaaaaccggg cttaataacc cccaaaacgg cgattggttc ccgtttttgc aaaggcttgc
     5701 gttggcgaaa tctcgcacgt aagtcattga acgtgttcat ggtctcgggt cggtcaaggg
     5761 gttgtcgacc tcgctcgatc atgcttacat tggtgtaact tagtcttggg gtcgcaactc
     5821 atgacattca tgaaaacagg ggagagcgga cgtcggatcc gcaacatcag cgagctggcc
     5881 aagctcgccg gtgtatcggc cggcaccgtt tcgcgggcgc tggccggcaa ggatctcgtc
     5941 aaccgcaaca cgcgcgaacg catccaggcg ctcgcccgcg aacacgggtt tcggcccaac
     6001 cagatggcga gccgcctgcg caccgggcat accggggtga tcggcgtggt cattccgctc
     6061 ggccacgaac gcgcgcagct gctctccgat ccgttcttca tgacgctcat cggccatctc
     6121 gccgacgccg tgaccgagcg cggctatgac ctgatgctga gccggatgat cccgctcgac
     6181 gaggaagact ggctcgagcg gatcaccgat tccggcatgc tcgatggcgt cgtgctgatc
     6241 ggccagtccg accagttcgc cacgatcgag cgcgttgcgg ccgattacct gccgctcgtc
     6301 gcctggggca cgcaccagcc cgggcagcgg cactgctcgg ttggtaccga cagcttcgtc
     6361 ggcggccaga tggctgcccg ccacctgctg gagcgcggcg cgcggcggct ggccttcttc
     6421 ggcgagacca ccggcatcga gatcgcccag cgccttgagg gcgcccgggc cgccgtcgcc
     6481 gagagcggca ccggcgccac gctggaagcg ttccccaccc ggctggcggt taacgccatg
     6541 gccgaccatg tgggcgagca tctcgacgtc atggaccggg caacggacgg catcttcgcg
     6601 gcctccgatg cgatcgccat gactgtcttg cgcatgctcc acgagcgcgg gcgcaaggtt
     6661 cccgacgaga tcgcggtgat cggctacgac gacctgccga tcgccatgca gaccatgccg
     6721 cgcctgacca cggtgcgcca ggacatcgcg gcgggcgccg cggccatggt cgacagcctg
     6781 ttccgccgca tcggcggcga ggatgccggc ccgaccgtgc tcgagccgcg agtggtcgtg
     6841 cgcgaaagcg cctgatcacc ggcaggcgcg cgcagcgccg ggccgcaagg cctaccccgc
     6901 tgcgactacc ctcgcctatg gctcggcaag tctcgccacc cctcccgctg gcgggaggtg
     6961 agacgcgtaa tctcatggtg ggcactcggg ccggacccga ggggagctcg cgcccttccc
     7021 tgcaatgctg tgggcggcta tcccggcagg gatgacagac cgtccgctca ttgcgctgct
     7081 ggccgccggg cgcgccagcc ggtttggcgc cgacaagctg gcggcagcct gtgccggcgg
     7141 cccgctcgcc ggccacgccg tggcggcggt gatggcgaca gggctgccgg cgctgtgcat
     7201 cacggccccc ggcgcccggc ccgactggct gcccgccgac atccccgccg tgcccaatcc
     7261 ggacgcggcc gagggcctgg cgacgtctgt ggccctcgcc gcccgcgagg cggcgcgccg
     7321 gggcgctccc agcttgctgc tccatcttgc cgacatgccc tgcgtcaccc ctgccctgct
     7381 cctggccgtg gctgaagccg gggcgatggg cgcctgccgc tatccctccg gccgcccggg
     7441 ggtgcccgcg cggtttccgg cagaccgctt tgccgagctt gcgtcactgg ccggtgaccg
     7501 gggcgcggcg gcgctgctcc aggccgatcc cgctggcgtg ctggtcgact gcgcggcgga
     7561 cgatctcatc gatgtcgaca cgcccgaagc gctcgcccgg gccgaagcgg tcctgaactt
     7621 gcgccagcgc aaggctggac aagcctcctc cggctagtca ctgtccaggc cgagggaagc
     7681 aatggcgcac tattccggac ctgacattgc cgcggtggag agcgggtttg gctcgccgct
     7741 cggcaatgtt ctgcggatcc ttgcctacat ggccgtggtg ttcgtatcgg ccacgctcgg
     7801 cttcatggcc gccggctggt cggtcggtga tgccgcctac atggtcactc tgaccatctt
     7861 c
//
LOCUS       DATNBV010000297         5330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2387632,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000297 DATNBV010000000
VERSION     DATNBV010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2387632"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="VN627_07185"
     CDS             complement(<1..1537)
                     /locus_tag="VN627_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXD03076.1"
                     /translation="MNRAGRDVMALGIGMAAIIMFVGTGGNIMPQVVRSLAGVGLPPD
                     RLLSIALILNIALIIFGLRRYRDLTDEVRERRRAENKARELAETDPLTGLLNRRSIAP
                     ATDDLLAQCAETGEHVAFVMIDIDNFKLINDARGHSTGDAILQVCAQRIQALLPDRGL
                     AARLGGDEFACVVPFHPLRIERIDDFAAQITSDVARPVELDGANIEVTVSVGISRSDV
                     GSDLSRPVNAQELLHMADVAMYQAKRAGKNRHFWFERPMEHELRFRSELEQAMRAGIP
                     AGEFVPYYEQQIDLVSGKIVGFEMLARWQSPVFGLVGPEVFIPVAEEIGIIAEMSEGL
                     IRQALADARDWSPDIRLAVNISPIQLRDPWFAQKILKLLVEANFPPSRLEIEITESAL
                     HENVGLVRTLITSLKNQGVSVSLDDFGSGYSSLAQLRSLPFDRLKIDRSFVTAMPDSR
                     DSATIVQAITSLGEGLGLPITAEGVESAEVVSELQRLGQFNAQGYLYGRPEPAEKTRA
                     RLAE"
     gene            complement(1724..2662)
                     /locus_tag="VN627_07190"
     CDS             complement(1724..2662)
                     /locus_tag="VN627_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HXD03077.1"
                     /translation="MTLSTLPVISLGAGADELPGQLGASFREFGFAMVSDHGIDAGLI
                     DAAWQLTAEFFALPEAEKRKHFRAELFGARGYTPFGREIAKGARHSDLKEFWHVGRDL
                     PADHQLTNASMPPNVWPARPEGFRETFQALYAEFDRVGARLLSHIAVHLGLAADWFEG
                     AVAEGNSVLRLLHYPPLPGDPGGAIRAGAHEDINLITLLLGAEEAGLELLARDGRWLA
                     VNPPPGALVINIGDMLQRLTNHVLPSTTHRVRNPDAGRAGFSRYSMPFFVHLRSDFRF
                     FTLPQCVTAANPDRYPESITADDYLQERLREIGLKG"
     gene            2731..3168
                     /locus_tag="VN627_07195"
     CDS             2731..3168
                     /locus_tag="VN627_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HXD03078.1"
                     /translation="MTKPENSVPSGRLWLMKSEPDAYSWDDLVAEGEGTWDGVRNHRA
                     ANNMRAMEVGDQAFFYHSNIGKEIVGIIEISQSGLTDPTDPEGKWACVKVKPVRKLAS
                     PVTLAEIKARPDLQDMELIRLGRLSVAELKPEEWAKVLEMAGE"
     gene            complement(3169..3522)
                     /locus_tag="VN627_07200"
     CDS             complement(3169..3522)
                     /locus_tag="VN627_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03079.1"
                     /translation="MTRLAALTLMVPLALGGCVTFRTVDDGIARARLGEAVVRDGVRI
                     MPMGVLEDSRCPQGVACIQAGTVRITAEVDGSRTELALNQPAGRVTLVEVYPARRKDT
                     TYYPDEYRFGFRVSR"
     gene            3589..4236
                     /gene="thiE"
                     /locus_tag="VN627_07205"
     CDS             3589..4236
                     /gene="thiE"
                     /locus_tag="VN627_07205"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXD03080.1"
                     /translation="MADKEPYQIERLPTQLYLISPLEVGGAFPERLARALDAGPVAAF
                     QFRVKGLDEHECARLAEPLQRLCSDLDVTFIVNDSISLAKRLGADGVHLGQDDGDVKD
                     ARERLGRTAQIGVTCHASRHLAMEAGEAGADYVAFGAFFATTTKQVEHHAAPDILEWW
                     QTFFELPCVAIGGITPENCKPLADAGADFVAVSGSVWNGDEAAQIRRFAEVLGIA"
     gene            4307..>5330
                     /locus_tag="VN627_07210"
     CDS             4307..>5330
                     /locus_tag="VN627_07210"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXD03081.1"
                     /translation="MPRAWRPCLPFPAATFIAATLLTGACHDTGSGPQGFATATQDAS
                     ATPAAAAAVGDPLASHDGKADPVADPVQRPVMQTQVVLDRLGFTPGVIDGKPGLSTKN
                     AIAGFQEANGLPVTGEMDAATQAKLAPYAQIPATRVVTIPADFARGPFMPVPKEPADQ
                     AKMPALGYASLEEKLAERFHTTVATLAQLNPGGLPAGASGAPPPKPEGAAAPKPKASP
                     PPTITFRAGQQIRVPNVGHDAMDPAKLGSDRAWQDTLAALGVGTDQPVVDRVEVSKSG
                     GTLKAYSPNGKLVAVYTATMGSEHDPLPIGEWKIQGISRNPKFHYNPALFWDASPGDK
                     EAMLPAG"
BASE COUNT          942 a         1731 c         1790 g          867 t
ORIGIN      
        1 gttcagccag gcgggcgcgg gtcttctccg ccggttcagg gcgaccgtat aggtaaccct
       61 gggcgttgaa ctggccgagg cgctgaagct cgctgaccac ttccgcactt tccacgccct
      121 cggcggtgat cggcaggccg aggccttcgc caagcgaggt gatcgcctgg acgatggtcg
      181 cgctgtcgcg gctatcaggc atggcggtga cgaaggagcg gtcgatcttg agccggtcga
      241 acggcagact gcgcagttgg gcaagcgagg agtagcccga accgaaatcg tcaaggctga
      301 ccgaaacgcc ctggttcttg aggctggtga tcagcgtgcg aaccaggcca acgttctcgt
      361 gcaacgccga ttcggtgatc tcgatctcca gccggcttgg cgggaagttt gcttccacca
      421 gcagtttgag gatcttctgg gcaaaccatg gatcgcgcag ctggatcggc gagatgttga
      481 ccgccagccg gatgtcgggg ctccagtcgc gggcgtctgc cagggcctgg cggatcaggc
      541 cctccgacat ttccgcgatg atcccgattt cctcggccac cgggatgaac acttccggcc
      601 cgaccagccc gaacaccggc gactgccacc gcgccagcat ctcgaagccg acgatcttgc
      661 cgctgacgag atcgatctgc tgctcgtagt aggggacgaa ctcgcccgcc gggatgccgg
      721 cgcgcatggc ctgttccagc tcgctgcgga accgcaattc gtgttccatc gggcgctcga
      781 accagaaatg gcggttcttg cccgcacgct tggcctggta catcgcgacg tccgccatgt
      841 gcagcagttc ctgcgcgttc accgggcggg aaaggtcgct gccgacgtcg gaccgggaaa
      901 tacccaccga cacggtcacc tcgatgttcg cgccgtcgag ttcaaccggg cgcgcgacat
      961 cgctggtgat ctgggcggcg aagtcgtcga tccgctcgat gcgcagggga tggaacggca
     1021 ccacgcaggc gaattcgtca ccgccaaggc gcgcggccag tccacggtcg ggtagcaggg
     1081 cctggatgcg ctgggcgcag acctgcagga tcgcatcgcc cgtgctgtgg ccgcgggcgt
     1141 cattgatcag cttgaagttg tcgatgtcga tcatgacgaa ggcgacgtgc tcgccggttt
     1201 cggcgcactg ggccagcaga tcgtcggtgg ccggcgcgat cgaccggcgg ttgagcaggc
     1261 cggtgagggg atcggtttcc gccagttcgc gggccttgtt ttcggcacgg cggcgttcgc
     1321 ggacctcgtc ggtgaggtcg cggtagcggc gcaggccgaa gatgatcagg gcgatgttga
     1381 ggatcagcgc gattgacagc aagcggtccg gcggcaggcc gacacccgcc agcgagcgga
     1441 cgacctgcgg catgatgttc ccgccggtgc caacgaacat gatgatcgcc gccatgccga
     1501 tgccgagcgc catcacgtcg cgcccggcgc ggttcacact gtcatcatcc gtgccgggcg
     1561 ttccggcacc agcaggttgc tgcccctcag gcacaaccct gtccaacttg cgacccaaga
     1621 gggttccccc gtttattgcc cgggaatgta actggccgaa caggattgaa tttcggtaaa
     1681 cgcccgcaat tcctcaagca aatcagccga ccttgcgccg tggtcagccc ttgaggccga
     1741 tctcccgcag ccgttcctgg agataatcgt cagcagtgat ggactccgga tagcggtccg
     1801 gattggctgc ggtaacgcat tgcggcagcg tgaaaaaacg aaagtcgctg cgcaaatgga
     1861 cgaagaacgg catcgaatag cggctgaacc cggccctccc agcgtcggga ttgcggacgc
     1921 ggtgagtggt cgatggcagg acgtggttgg tcaggcgctg cagcatgtcg ccgatgttga
     1981 tgacgagcgc gcccggcggc gggttaacgg caagccaccg gccgtccctg gccagcagtt
     2041 cgagcccggc ctcctcggca ccgagcagca gggtaatgag gttgatgtcc tcgtgcgcgc
     2101 cggcgcggat ggcgccgccg ggatcgccgg ggagaggcgg gtaatgcagc agtcgcagga
     2161 ccgagttgcc ctcggccacg gcaccctcga accagtccgc ggcaaggccg agatgaaccg
     2221 cgatgtggga gagcaatcgg gcaccgaccc ggtcgaactc ggcatagagc gcctggaaag
     2281 tttcccggaa gccctcgggc cgtgccggcc acacgttggg cggcatcgag gcatttgtga
     2341 gctggtggtc cgccggcaag tcgcggccga catgccagaa ttccttgaga tcggaatgcc
     2401 gcgccccctt ggcgatctcg cgcccgaacg gggtatagcc gcgcgcgcca aacagctctg
     2461 cgcggaagtg cttgcgcttt tcggcctcgg gcagggcaaa gaactcggcg gtcagctgcc
     2521 acgccgcatc gatcagcccg gcgtcgatcc cgtggtcgct gaccatggcg aagccgaact
     2581 cgcggaacga ggcgcccaac tggccgggca gttcatcggc accggcgccg agcgagatta
     2641 ccggaagggt cgagagcgtc atgcctgtct tgcgtccgca ccatggttgg ctatttgacc
     2701 cggctatagc ggggacttgc gggagccgac atgaccaagc ctgagaattc tgtgccttcc
     2761 gggcgcctgt ggctgatgaa gtccgagcct gacgcttatt cgtgggacga tctcgtcgcg
     2821 gaaggcgaag gcacctggga cggcgtgcgc aaccaccgcg cggccaacaa catgcgggcc
     2881 atggaggtgg gggaccaggc gttcttctat cactccaaca tcggcaagga gatcgtcggg
     2941 atcatcgaga tcagtcagtc cgggctgacc gatccgactg atcccgaggg caagtgggcc
     3001 tgcgtgaagg taaagccggt gcgcaagctg gccagcccgg tcaccctcgc cgagatcaag
     3061 gcgcggcccg acctgcagga tatggaactg atccgcctgg gccggctgtc ggtggccgag
     3121 ctcaagcccg aggaatgggc caaggtgctg gagatggcgg gggaatgatc accggctgac
     3181 gcggaagccg aagcggtatt cgtcgggata gtaggtcgtg tccttgcgcc gggcaggata
     3241 gacctcgacc aatgtcacgc ggcccgcggg ctggttcagc gccagttcgg tccggctccc
     3301 gtcgacctcg gcagtaattc gcacagtacc cgcctgaatg caggcgaccc cctgcgggca
     3361 gcggctgtcc tccagcacgc ccatcggcat gatccgcacc ccgtcgcgca ccacggcctc
     3421 gccaagccgg gcgcgggcaa tcccgtcgtc gacggtgcgg aaggttacgc agccgccaag
     3481 ggcgagcggt accataaggg tgagggcggc aaggcgggtc attgcctgca agatcgcctg
     3541 atgcgatgaa cggttcccga acgcgggtcc ctgcgctaga gcgccgccat ggcggacaag
     3601 gaaccctacc agatcgaacg gctgcccacg cagctctatc tcatctctcc gctcgaagtc
     3661 ggcggggcct ttcccgagcg ccttgcccgg gcgcttgacg ccggcccggt ggccgccttc
     3721 cagttccgcg tgaaaggtct cgacgagcat gagtgcgccc ggctggcaga gccgctgcag
     3781 cgcctgtgca gcgacctgga cgtgaccttc atcgtcaacg attcgatcag cctcgcgaag
     3841 cgcctcgggg ccgacggcgt ccacctcggg caggacgatg gtgacgtgaa ggacgcgcgt
     3901 gagcggctcg ggcggactgc ccagatcggc gtgacctgcc atgccagccg ccacctggcg
     3961 atggaggcgg gcgaagccgg ggcggactat gtggccttcg gcgccttctt cgccaccacg
     4021 accaagcagg tcgagcacca tgccgcgcct gatattctcg aatggtggca aaccttcttc
     4081 gagctgccct gcgtcgccat cggcgggatc acgcctgaga actgcaagcc cctggcggat
     4141 gcgggggcag acttcgtggc ggtctccggc tcggtctgga acggcgatga agcagcgcaa
     4201 atccgccgct tcgcggaggt tctgggaatc gcctgagcga atggcgcgga aatatccgcg
     4261 ggcctgagtg ttgagttccc ggaccacaac gatccgggag accccaatgc cccgcgcctg
     4321 gcgcccctgc ctgccgttcc ccgccgccac ctttatcgcc gcaacccttc tcaccggtgc
     4381 ctgccacgac accggctccg gcccgcaggg cttcgccacg gcgacccagg atgcttcagc
     4441 aactccggcg gcggcggcgg cagtcggcga tccgctggct tcgcatgatg gcaaggccga
     4501 tcccgtggcc gatcccgtgc agcgcccggt catgcagact caggtggtgc tcgatcgact
     4561 cggtttcacg cccggagtga ttgacggcaa gcccggcctt tccaccaaga acgccattgc
     4621 cggcttccaa gaggccaatg gacttcccgt taccggcgaa atggacgcgg ccacccaggc
     4681 gaagctggcg ccctatgcgc agattccggc tacacgggtg gtcaccatcc ctgccgattt
     4741 cgcgcgcggg ccattcatgc cggtccccaa ggagccggcc gaccaggcga aaatgccggc
     4801 actgggctat gcctcgctgg aggaaaagct ggccgaacgg ttccacacga cggtggcaac
     4861 tctggctcag ctcaatcccg gcgggttacc cgcgggagcg tccggggcac cgccgccgaa
     4921 gccggaagga gccgctgcgc ccaagcctaa ggctagcccg ccgccgacca tcaccttccg
     4981 cgccggccag caaatccgcg tacccaacgt gggccatgac gccatggatc cggccaagct
     5041 gggctccgac cgggcatggc aggataccct ggcagcgctt ggcgtcggca ccgaccagcc
     5101 ggtggttgat cgggtcgagg tcagcaagtc gggcgggacg ctcaaggcct attcccccaa
     5161 cggcaagctg gtcgccgtct ataccgcaac tatgggttcg gagcatgatc cgctgcccat
     5221 tggcgaatgg aagatccagg gcatcagccg caatccgaag ttccactaca acccggcact
     5281 gttctgggac gcgagcccgg gtgacaagga agccatgcta ccggcgggac
//
LOCUS       DATNBV010000298         2327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4645704,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000298 DATNBV010000000
VERSION     DATNBV010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4645704"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VN627_07215"
     CDS             complement(<1..1093)
                     /locus_tag="VN627_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXD03082.1"
                     /translation="MGYVPQDDFGTPSVQSATAVTLTIDGRPVTVPEGTSVMRAAAEC
                     GGAIPKLCATDSLQSFGSCRLCLVEVEGMRGTPASCTTPVAPGMVIHTQTPRLEKLRR
                     GVMELYISDHPLDCLTCSANNDCELQDAAAQVGLRDVRYGYAGENHLAAPTDVSNPYF
                     DFDASKCIVCSRCVRACEEVQGTFALTIDGRGFGSKVSAGQATDDFLSSECVSCGACV
                     AACPTATLNEKAVAEIGKPERAVVTTCAYCGVGCTFRAEMKGEQLVRMVPWKDGKANH
                     GHSCVKGRFAWGYANHQDRIMQPMMREKITDPWREVSWEEAISHTAAEFRRIQKDYGN
                     HAIGGITSSRCTNEETFLVQKLVRAGFGNN"
     gene            1221..2261
                     /locus_tag="VN627_07220"
     CDS             1221..2261
                     /locus_tag="VN627_07220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXD03083.1"
                     /translation="MQDLLFNAMGAVGRLSLNRPKAIHALTRDMCLAMTSALDRFAAA
                     PSVTTIIIDHAAGRGFCAGGDIAMMRHSVLNDGGEAARQFFFDEYRLNHQMFTYPKPV
                     VTFMDGITMGGGVGIALPARFRVATENTRLAMPEAAIGLFPDVGGGWFLSRLQHRIGQ
                     YLAISDSRINGADCLWLGLATHYLPADQVAEAKRRMTMGEDAEAVLDELAIAPPPAPI
                     EAQAAQIRQHFSHERLEDILASLAADESDWARATRATILEKCPMTCKVALRQLARARV
                     LTDFADNMAMEYRIACRMIMRPDFAEGVRAVILDKDRAPKWDPPRAEEVSDDAVDAIF
                     APLPADAEWRAR"
BASE COUNT          412 a          746 c          776 g          393 t
ORIGIN      
        1 tgttgttgcc gaagccggca cggaccagct tctggacgag aaaggtctcc tcgttcgtgc
       61 agcgcgacga ggtgatcccg ccgatcgcat ggttgccata gtctttctga attctccgga
      121 attcggcggc ggtgtggctg atcgcctcct cccaggacac ctcgcgccat gggtcggtga
      181 tcttctcgcg catcatcggc tgcatgatgc ggtcctggtg attggcatag ccccaggcga
      241 agcggccctt gacgcaggag tggccgtggt tcgccttgcc gtccttccac gggaccatgc
      301 ggacgagctg ttcgcccttc atctcggcgc ggaaggtaca gccgacgccg caataggcgc
      361 aggtggtgac gaccgcgcgc tcgggcttgc cgatctcggc cacggccttt tcgttgagcg
      421 tggccgtggg acaggccgcg acgcaggccc cgcacgagac gcattcagag gagaggaaat
      481 catcggtggc ctgaccggcg ctgaccttcg agccgaaccc gcgcccgtcg atcgtcagcg
      541 cgaaggttcc ctgcacttcc tcgcaggcgc gcacgcagcg cgagcagacg atgcacttgg
      601 aggcatcgaa atcgaaatag ggattggaaa catcggtcgg cgcggcgaga tggttctccc
      661 cggcatagcc ataacgcaca tcccgcaggc ccacctgcgc cgcggcatcc tgcagttcgc
      721 aatcgttgtt ggcgctgcag gtcaggcagt cgagcgggtg gtcggagatg tagagctcca
      781 tcaccccccg gcgcagcttt tccagccgcg gggtctgggt atggatgacc atgccaggcg
      841 cgacgggcgt ggtgcagctg gccggcgtgc cgcgcatccc ttcgacctcg accaggcaca
      901 ggcggcacga gccgaaggac tggagcgaat cggtggcgca aagcttggga atggccccgc
      961 cgcattccgc cgccgcgcgc atgaccgacg tgccttcggg cacggtgacc ggccggccat
     1021 cgatcgtgag ggtgacggct gtggccgact gcaccgaagg agtgccgaaa tcgtcttgcg
     1081 ggacgtagcc catgaaatct ccgagattct cgtttgacgg ccaatatagc gcacatggcc
     1141 gcaaaattcg agttgccgaa tgggcatggc ttgcgcaagg cggagcgcaa agtgaacgca
     1201 aacgtgcggg agtgagggat atgcaggacc tgctgttcaa cgcaatgggc gccgtcggca
     1261 ggctttcgct caaccggccc aaggcgattc acgcgcttac ccgcgacatg tgcctggcga
     1321 tgacctcggc gctcgaccgg ttcgccgcgg cgccgagcgt gacgaccatc atcatcgacc
     1381 atgccgcagg tcggggcttt tgcgcgggcg gagacatcgc gatgatgcgc cactcggtgc
     1441 tgaacgacgg cggcgaagcg gcgcggcagt tcttcttcga cgagtatcgt ctcaatcacc
     1501 agatgttcac ctatcccaag ccggtggtga ccttcatgga cgggatcacc atgggcggcg
     1561 gcgtcggcat tgcgctgcct gcgcggtttc gggtggcgac tgaaaacacc cgcctcgcca
     1621 tgcccgaggc tgccatcggg ctgttccccg acgtgggcgg cggctggttc ctctcgcggc
     1681 tccagcaccg gatcggccag tatctcgcga tcagtgactc ccggatcaac ggagccgatt
     1741 gcctgtggct gggcctggcc acccattacc tcccggccga tcaggtggcc gaagccaagc
     1801 gccggatgac catgggcgag gacgccgagg ccgtgctcga cgagctggcc attgcgccgc
     1861 cccctgcccc gatcgaggcc caggccgcgc agatccggca gcacttcagc catgagcggc
     1921 tggaagacat cctcgcctcg ctcgctgctg acgagagcga ctgggcccgg gccacccggg
     1981 cgacgatcct cgaaaagtgc ccgatgacct gcaaggtggc cctgcgccag ctcgcccggg
     2041 cccgcgtgtt gaccgacttc gccgacaaca tggcaatgga ataccgcatt gcctgccgca
     2101 tgatcatgcg ccccgatttc gccgagggcg tgcgcgcggt gatccttgac aaggatcgcg
     2161 caccgaagtg ggacccgccg cgcgccgagg aggtgagcga cgatgccgtg gacgcgatct
     2221 ttgcgccgct gcccgccgat gcggaatggc gagcgcggta aagcgatcgc ggtgagacag
     2281 tcagcaatta ccagacggta aaagggagga actaaaatgc agattac
//
LOCUS       DATNBV010000299         2519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4004015,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000299 DATNBV010000000
VERSION     DATNBV010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4004015"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..540
                     /locus_tag="VN627_07225"
     CDS             271..540
                     /locus_tag="VN627_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03084.1"
                     /translation="MSYWRDRARSVIAELTRDLPADAPLEQRRKALWGKGHAAHGGTS
                     WGRKMWGKEVRKYLSSHGDSYARGTTTPINWPADIHFPFREGSDG"
     gene            533..997
                     /locus_tag="VN627_07230"
     CDS             533..997
                     /locus_tag="VN627_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03085.1"
                     /translation="MAKAKAHPNQLALDLSVPRPATGVAALAGLEARICSTVGVLLAS
                     DGRPREIIAAEMSVLLDETVSRAMLDAYSSPARIDHRVPMNRFWALVAVTGRLDLLDP
                     LVREIGGAVLIGDEVKTARLGQLQQIMARAQAEMRQLKAEAPDMSRMGGKQG"
     gene            994..>2519
                     /locus_tag="VN627_07235"
     CDS             994..>2519
                     /locus_tag="VN627_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HXD03086.1"
                     /translation="MKPSTFSTAGGAIATIATDTGPEPGETGPRDWFGAAELAALALP
                     GLPADKRSLNRRAKAERWDVRCDSAGEPLARLRRRNGGGVEYHVSLLPADTRLELARR
                     GLSDSRPEPRAAEAPAVAAWRWYEAQTAKVKAEAERRATIIATVDLLLAAGMTKTAAV
                     AEASRRHGVGKATVWEYLDLVEGVARHDRLPALAPKRKGGGREADISDQVWQIFLSDY
                     LRPEKPTLTSCYDRAAAIAASRGEAMPSERSIRRKLDRDVDARVILLNRGGKDEFERS
                     IPDARRTLDGLHAMHIVNVDGHQFDVFVKSPEGKIVRPVLVAIQDVYSSKMLAWRIDL
                     SENYLATRLAFADLFRNFGIPKICLLDNSRTFAGKQLTGGAKTRYRYKVKEEEPAGLL
                     VSLGIEVRFATIYHGQSKPIERAFRDISDRISRGPECAGAYTGNRPTAKPANYGSRAV
                     DWDVFVQVVDRGIALHNAKLGRRGRDYRGRSFDQVFADSYAAAPIGKATPEQLRMALL
                     A"
BASE COUNT          435 a          868 c          880 g          336 t
ORIGIN      
        1 agcgagcccg ccggatgcag gaaaagcgcc tggcaaagga gcgggcgctg gaacaacgcc
       61 gtcggaccat cgccgagatt gccgagcgcc tgaaggcgcg gcagctcgag gccgggcaat
      121 gatcgcgccc accgccgccc cgcgccgcaa atcggactgc ccgctggccg ggaccaagga
      181 gccggtcggg cggtgcacgt tctggtggga atcgtgcccg gacaaggtga agaactgcga
      241 gctccgccga cgcaacgcgg aggcccagtc atgagttact ggcgcgaccg tgcccgttcg
      301 gtgatcgccg agttgacccg cgacctgccc gcggacgcgc cgctcgaaca gcgacgcaag
      361 gcgttgtggg gcaagggcca tgccgcgcat ggcggcacca gctggggccg caagatgtgg
      421 ggcaaggaag tacgcaagta cctttccagc cacggcgaca gctatgcccg gggaacaacc
      481 acgccgatca actggccggc tgacatccac ttcccgttcc gggagggcag cgatggctaa
      541 ggccaaggcg catcccaacc agctggcgct tgacctgtcc gtgccgcgac cggcgaccgg
      601 cgtcgcggcg ctggccgggc tcgaagcgcg catctgctcc acggtcgggg tgctgctcgc
      661 cagcgacggc cgcccgcgcg agatcatcgc cgccgaaatg agcgtgctgc tcgacgaaac
      721 ggtaagccgc gcgatgctgg atgcctattc gagcccggcc cgtatcgatc accgggttcc
      781 gatgaaccgt ttctgggcac tggttgcggt caccggccgg ctcgatctgc tggacccgct
      841 cgtgcgcgag atcggcggcg cggtcctgat cggcgacgaa gtgaagacgg cgcggcttgg
      901 acagctgcag cagatcatgg cccgggcgca ggcagaaatg cgccagctca aggccgaggc
      961 gccggacatg agccgaatgg ggggcaaaca ggggtgaagc cgagcacgtt ttccactgcc
     1021 gggggggcaa tcgcaacaat cgccacagac accggaccgg agccgggcga gaccggcccg
     1081 cgcgactggt tcggcgcggc cgagctggcg gcgctggcgc tgccgggcct gcccgccgac
     1141 aagcgttcgc tcaaccgccg ggccaaggcc gagcgctggg acgtgcgctg cgacagcgcc
     1201 ggggaaccgc tggcgcggct gcgccggcgc aacggcggcg gggtggaata ccacgtttcg
     1261 ctgctgcctg ccgacacccg gctggagctg gcccgacgcg gcctttcgga cagccggccg
     1321 gagccgcgcg cagccgaggc tccggccgtc gccgcgtggc gctggtacga ggcgcagacc
     1381 gccaaggtga aggccgaggc cgagcgccgg gcgacgatca tcgccaccgt cgacctgctg
     1441 ctggcggccg gaatgacgaa gacggcggcg gttgccgaag cctcgcgtcg gcacggcgtg
     1501 ggcaaggcca cggtgtggga atacctcgac ctggtcgaag gcgtcgcccg gcacgaccgg
     1561 ctgccggcgc ttgcccccaa gcgcaagggc ggcgggcgcg aggccgatat ttccgaccag
     1621 gtctggcaaa tctttctcag cgattatctg cgccccgaaa aacccactct caccagctgc
     1681 tacgaccggg ccgcggcgat cgccgcgtcg cgcggcgaag cgatgccatc ggaacggtcg
     1741 atccggcgca agctcgatcg cgacgtcgat gcgcgggtta tcctgctcaa ccgcggcggc
     1801 aaggatgagt tcgagcgctc gatcccggac gcccggcgca cgctcgacgg gctgcacgcg
     1861 atgcacatcg tcaacgtcga cggtcaccag ttcgacgtgt tcgtgaagtc gcccgagggc
     1921 aagatcgtgc gcccggtgct ggtcgcgatc caggacgtct acagctccaa gatgctggca
     1981 tggcggatcg acctgtcgga aaactacctc gccacccggc tcgcctttgc cgacctgttc
     2041 cgcaatttcg gcatcccaaa aatctgcctt ctcgacaaca gccggacctt cgccggcaag
     2101 cagctgaccg gcggtgcgaa gacccgctac cgctacaagg tcaaggaaga ggagccggcc
     2161 ggcctgctgg tgtcgctggg gatcgaggtc cgcttcgcga cgatctacca cggccagtcg
     2221 aaaccgatcg aacgcgcctt ccgcgacatt tccgaccgaa tttcgcgcgg gccggagtgc
     2281 gccggcgcct ataccggcaa ccgcccgacc gcgaagccgg cgaactatgg cagccgcgcc
     2341 gtggactggg atgtcttcgt ccaggtcgtc gaccgcggca tcgccctgca caacgccaag
     2401 ctcggccgac gcgggcgcga ctaccgcggg cgcagcttcg accaggtctt cgccgacagc
     2461 tatgccgccg cgccgatcgg caaggccacg cccgagcagc tgcgcatggc gctgctcgc
//
LOCUS       DATNBV010000300         2597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3666174,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000300 DATNBV010000000
VERSION     DATNBV010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3666174"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="VN627_07240"
     CDS             complement(<1..130)
                     /locus_tag="VN627_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HXD03087.1"
                     /translation="MNDPADRYDYIVVGGGSAGCVLARRLTDDGRSRVLLLEAGEDD"
     gene            complement(174..665)
                     /locus_tag="VN627_07245"
     CDS             complement(174..665)
                     /locus_tag="VN627_07245"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD03088.1"
                     /translation="MVPRKRAECGICHPHFNLDHGIVGQLLAAPENLISGSDAGAHYQ
                     MFSGAGDSTLVLTRHVRDRGDLPLELAVRKLTGDQADLLGLADRGRIAQGAIADITVF
                     ALDELNWAAETIVHDVPGDHPRFTRAPGGYRYTLVNGQVVQQDGQANGVLAGRFDSEL
                     VAA"
     gene            complement(566..1846)
                     /locus_tag="VN627_07250"
     CDS             complement(566..1846)
                     /locus_tag="VN627_07250"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD03089.1"
                     /translation="MNLLIKGGHVIDGTGAAAFPADVRVSGSRIVELGPDLPAHDNEE
                     VLDARGAYVTPGFIDTHTHFDASLYWDPACDPMLSHGVTTVLIGNCGLGLAPVRSADR
                     EQLCSLFAYVEDLPIDLLRAKVPWTWEDFRTYAQDLAGREFGPNVAVLVSHSLLRMWA
                     MGEAAWSRAADAAEREAIAGLLARALAAGASGLSSSFYDRTPDGVMVPSVHADDAEFT
                     AIFETLGRHGGVFQTIPSLWDVDLAIKDLERVAELAKPFGVPVISNGIFERPEDHSSH
                     ERMVASARRLNASGARLQHLASPRSIELPIRVRDCFPLGTVAAWSEVVSAATGKEAML
                     ADPAWRARARKDWDGASYSKQARSTRIVKVGKPELSSWIGRMLSDLLAERGGHPSDVM
                     ADWFLENELNAEFVIPISTLITASSGNSWPRPKT"
     gene            complement(1843..>2597)
                     /locus_tag="VN627_07255"
     CDS             complement(1843..>2597)
                     /locus_tag="VN627_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXD03090.1"
                     /translation="PVWVFMELAGLPLDQFDHLRGLTLDFLREADEAKRQPIIEQILE
                     STAAVVRARQAKREKDLISRLLDSTIDGRPITFDEMQSICLFFVLAGLDTVTSALGFG
                     FQYLAKDPALQQRLREHPELIDIAAEELLRIGAPSSVIRHAARDLEFRGVSMRQGDRI
                     HILYPAANLDPSVFADPLQVNLQQTEPTVAFGTGPHRCLGSHLARIEMRILYREWLRR
                     VPTFSLDAERPAKYHTGLVWGISELHLKWSGA"
BASE COUNT          468 a          853 c          846 g          430 t
ORIGIN      
        1 catcgtcctc ccccgcttca agcagcagca cgcgtgaccg gccgtcgtcg gtgaggcggc
       61 gggccagcac gcaaccggca gaaccgcccc cgacaacgat atagtcatag cggtcggccg
      121 gatcgttcat tttcgctgtc cgccgtgatc acacgggttg ggccagagcg tcctcatgcc
      181 gcaaccagtt cgctgtcgaa cctcccagcg agaacgccgt tggcctgccc atcctgctgg
      241 acgacctggc cattgaccag cgtgtaccgg tagccccccg gcgcgcgcgt gaagcgggga
      301 tggtcgccgg gaacgtcatg aacaatggtt tcggcggccc agttcagctc gtcgagcgca
      361 aataccgtga tatcggcgat ggccccttgg gcgatgcggc cgcgatcggc gaggccgagc
      421 agatcagcct gatcgcccgt cagcttcctc accgcgagtt ccagcggcag gtcaccccgg
      481 tcgcggacgt gacgggtgag cacgagggtg gagtcccctg cccccgaaaa catctggtaa
      541 tgcgcgcccg catcgctgcc gctgatcagg ttttcgggcg cggccaggag ttgcccgacg
      601 atgccgtgat caaggttgaa atggggatga caaattccgc attcagctcg ttttcgagga
      661 accagtcggc catcacatcg ctcggatgac cgccgcgctc ggcgagaaga tccgacagca
      721 tgcgcccgat ccagctgctg agctccggtt tgccgacctt cacgatccgc gtggaccggg
      781 cctgcttcga atagcttgcg ccgtcccagt ctttcctcgc cctggcgcgc caggcgggat
      841 cggcgagcat ggcttccttt ccagttgccg cgctcaccac ctcggaccag gcggccacgg
      901 tgccgagcgg aaagcaatcg cgcacccgga tcgggagctc gatggagcgg ggggacgcca
      961 ggtgttgcag ccgtgcgccc gaagcattca gccggcgcgc actggccacc atgcgttcgt
     1021 gcgacgaatg gtcttccggc cgttcgaaga taccgttgga gataaccggc acgccgaacg
     1081 gcttggcaag ttcggcaacc cgctccaggt ccttgatcgc cagatccaca tcccacaggc
     1141 tggggatggt ctgaaacacg ccgccgtgcc tgccgagcgt ttcaaaaatg gccgtgaact
     1201 ccgcatcgtc ggcatggacg ctggggacca tcacgccgtc aggagtgcga tcgtagaatg
     1261 aggaggacag gccgctggct cctgctgcca aggccctggc cagaagcccg gcgatggcct
     1321 cgcgttccgc agcatcggcg gcccgcgacc aggccgcttc acccatcgcc cacatccgca
     1381 gcagcgagtg gctgaccagc acggcgacgt tcgggccgaa ttcgcggccg gccaggtcct
     1441 gagcgtaggt ccggaagtct tcccatgtcc agggcacctt tgccctgagc agatcgatcg
     1501 gcagatcctc gacataggcg aacagcgagc agagctgttc gcgatccgcc gaacggaccg
     1561 gcgccagtcc caggccgcaa ttgccgatga gcaccgtggt gacgccgtgc gacagcatgg
     1621 gatcgcaggc gggatcccaa tacagcgaag catcgaaatg ggtgtgagtg tcgatgaagc
     1681 cgggggtgac ataggccccg cgcgcatcca gcacctcctc gttgtcgtgg gccggcaggt
     1741 cggggccaag ctcgacgatc cggctgccgc tcacgcgcac gtccgccggg aatgccgcag
     1801 caccggtgcc atcgatcaca tggcccccct tgatcagcag gttcatgcac cgctccattt
     1861 caggtgtagt tcgctgatgc cccagacgag gcccgtgtgg tacttggccg gccgctcggc
     1921 atcgagggag aaggtgggca cccgccgcag ccactccctg tacaggatgc gcatctcgat
     1981 ccgggccaga tgcgatccca ggcagcgatg cgggccggtg ccgaaggcta cggtcggctc
     2041 ggtctgctgc aggtttacct ggagcggatc tgcaaaaacc gaggggtcga ggttggccgc
     2101 gggatagaga atatggatgc ggtcaccctg gcgcatggat acgccgcgaa actccagatc
     2161 acgcgcggca tggcggatga ccgagctggg cgccccgatc cgcagcagtt cctctgccgc
     2221 gatgtcgatc agctccgggt gttcgcgcag ccgctgctgc agcgcgggat ccttcgccag
     2281 gtactggaag ccgaacccca aggcacttgt aaccgtgtcc agcccggcaa ggacgaagaa
     2341 caggcagatc gactgcatct catcgaatgt gatgggccgg ccgtcgatcg tggaatcgag
     2401 caggcggctg atcaggtcct tctcccgctt ggcctggcgg gcgcggacga ccgccgcggt
     2461 tgactcgagg atctgttcga tgatcggctg gcgcttcgct tcgtcagcct cgcgcaggaa
     2521 atcgagggtc agcccgcgca gatgatcgaa ctggtcgagc ggcagtccgg caagctccat
     2581 gaacacccag accggca
//
LOCUS       DATNBV010000301         4739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2734955,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000301 DATNBV010000000
VERSION     DATNBV010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2734955"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1976
                     /locus_tag="VN627_07260"
     CDS             <1..1976
                     /locus_tag="VN627_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXD03091.1"
                     /translation="RSEFRGAEWDWELSAGKRWAAQANVRGAPADYPMDLAECDIQIM
                     NFNGVGGGTILYNAVWPRLMPEHFRERSLYGVAADWPLDYAELLPWYEATDREMGVAG
                     LGGNPVYPPGAEPPNPPLPFGPGAMAVARALNRRGWHWWPDTIAINSVPYGGRHACIQ
                     RGTCAQGCGEGAKASTDATHWQPFVAAGGRVITGARVHQVTLDERGLASGAVWVDAAG
                     RDHFQAARAVLVAANGIGTPRLLLNSACERFPAGLANSSDQLGRNLMLHPVAAVIGTF
                     PEQLEGWQGQYGSTVQCQQFGLTDQRRGFVGGCKWSLHPLGSGPVAESVLQLAQGDPA
                     TYHERVGRRLGHKLMWSIMSEDMPVPENRVVLSGDLCDSSGMPAPRVLYCGGADVAAN
                     LDFNVAQAEAVFREAGAWEVTSYNPAGQQAHLMGTARMGDDPASSVVDRWGMTHDVPN
                     LGVIDGSVFVTSGPVNPTTTIMALAARTADHLARNFGRIAPEQARRTWLAPLRPTRKT
                     DHAPVAEAAPLAWTDERAAAFAAIADTLVPAVAELPAAGQLTLERGTARQAYSARPDL
                     HAALDRALAAGPDGFADLIAADPAAWSAMAMLLSAAYFAVPETGARHGYFGQQPRPAQ
                     PDRYPAYIAEGLIDHMLVPGWRERWAAGDPVAA"
     gene            2125..2880
                     /locus_tag="VN627_07265"
     CDS             2125..2880
                     /locus_tag="VN627_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXD03092.1"
                     /translation="MASASLSLAPGMSPGEWQTRVELAACYRLIALHGWDDLIATHIS
                     ARVPGTRDFLINRLGLTFEEITASNLVRIDLDGNVTDGSDAPINRAGFTIHSAVHQVR
                     EDAGCVIHLHTLDGTAVSALEDGLLPLNQIAMLAHGDIAFHEYEGVAFDLAERERLQA
                     DLGQSNLMLLRNHGTLAVGRTVGEAYTRMYTLERACSIQVRTLGMNRPLHAADAGVIE
                     RTGDLGRDAASYAAVAWPAALRRAGRLSPGFDS"
     gene            2884..3837
                     /locus_tag="VN627_07270"
     CDS             2884..3837
                     /locus_tag="VN627_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03093.1"
                     /translation="MADRSAIVVASQFGDEANAAIASLVPGVRIRPVPLGYQAPDILD
                     ADVFLHIPDVRTERGGSSERPAGWPGRLRLVQVISTGTDFYPDWLFAGPAVTTAQGLA
                     GPAISEYCLAAIFAAARDMPRIWMAGPQDWRRLQCQQVSGSTLGLFGFGSIGSTLARK
                     ALALGMRVAAVRRSREPLGIDGVEQLDQLGALLARCDHVVLAAPGTAETRHVVNRETL
                     AMARPGLHLVNVARGGLIDDNALLASLDNGTVGRATLDVTEPEPLPAGHRFYTHPRVF
                     LSPHIANSGPAFLAAISRRAADNIKRLEAGGELVGVSAARV"
     gene            complement(3839..>4739)
                     /locus_tag="VN627_07275"
     CDS             complement(3839..>4739)
                     /locus_tag="VN627_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXD03094.1"
                     /translation="RLERKNAKLTRVRVRRAVVLSAGGFAFNRTMMQRLAPEFMGMAP
                     LGTFGDDGSGIKLGASVGGATDKLGDCSAWRFLYPPVAWTTGVVVGTAGERIVNEELY
                     GARTGDALFRKSGGRGWVIGDQPLHDLVQEQRQDPELQPYMKMQLRAQMQFGLARANT
                     LPELAAKIGVPADALVRTVESYNAAIRAGQPDAWGKADSYRRLIETGPFYAYNMSYDS
                     KMNPIMGITMGGLKVNEDSGAVLDDAGAPVPGLYAAGRTAIGVCSNFYVSGLSLADCV
                     FSGWRAADSIKAGATAAAPANAA"
BASE COUNT          705 a         1661 c         1621 g          752 t
ORIGIN      
        1 atcggagcga gtttcgcggg gcggagtggg actgggaact ctcggccggg aagcgctggg
       61 cggcacaggc caacgtgcgc ggcgcccctg ctgactaccc catggacctg gccgaatgcg
      121 acatccagat catgaacttc aacggggtgg gcggcggcac catcctgtat aatgccgtgt
      181 ggccccggct gatgcccgag catttccggg aacgctcgct ttatggcgtg gcagcggact
      241 ggccgctcga ctacgccgag ctgctgccat ggtacgaggc gaccgaccgg gaaatgggcg
      301 tggcggggct gggcggcaat ccggtctacc cgcccggcgc cgagccgccc aatccgccgc
      361 tgccgttcgg gccaggcgcc atggcggtcg cccgcgcgct caatcggcgc ggctggcact
      421 ggtggcccga taccatcgcg atcaattcgg tgccctatgg cgggcgccac gcctgcatcc
      481 agcggggcac atgcgcgcag ggctgcgggg agggtgccaa ggccagcacc gacgcgaccc
      541 actggcagcc cttcgtcgcg gcggggggaa gggtgatcac cggggcccgc gtccatcagg
      601 tgaccctgga cgagcgaggc ctcgcgagcg gtgccgtgtg ggtcgatgcg gcaggccgcg
      661 accacttcca ggctgcccgc gcggtgctgg tcgccgccaa tggcatcggt acgccgcgcc
      721 tcctgctcaa ttccgcctgc gagcggtttc ccgccgggct ggccaattcg tccgaccagc
      781 tcggccgcaa cctgatgctg cacccggtcg cggccgtcat cggcaccttt cccgagcagc
      841 tggagggctg gcaggggcag tacggctcca ccgtccagtg ccagcagttt ggcctgaccg
      901 accagcgccg cggcttcgtg ggaggatgca agtggtcgct ccatccgctg ggcagcgggc
      961 ctgtcgccga atccgtgctg caactcgccc aaggcgatcc cgcgacctat catgagcggg
     1021 tcggccggcg cctcggccac aaactgatgt ggtcgatcat gagcgaggac atgcccgtgc
     1081 cggagaaccg cgtcgtcctt tcaggcgacc tgtgcgacag ctcgggcatg ccggcgcccc
     1141 gcgtgctcta ttgcggcggg gccgacgtgg ccgccaacct cgacttcaac gtggcgcagg
     1201 ccgaggcggt gttccgcgag gccggcgcct gggaagtgac cagctacaat ccggccgggc
     1261 agcaggccca cctgatgggg acggcgcgga tgggcgacga tcccgccagt tcggttgtgg
     1321 accgctgggg catgacccat gacgtgccca atctcggcgt gatcgacggg agcgtcttcg
     1381 tgacctccgg gccggtgaac ccgacgacca cgatcatggc cctggccgcc cgcaccgccg
     1441 atcacctggc ccgcaacttc ggccggatcg ccccggagca ggcccggcgg acctggctcg
     1501 cgccgctgcg tcccacccgc aagaccgatc atgcgccggt tgccgaggct gcaccgctgg
     1561 cgtggacgga cgagcgggcg gccgctttcg ccgcgattgc cgatacgctt gttccggcgg
     1621 tggcggagct gcctgctgcg ggccagctca ccctggaacg ggggacagcg cggcaggcct
     1681 atagcgctcg ccccgatctc cacgctgcgc ttgaccgcgc gctagccgcc ggcccggacg
     1741 gcttcgccga cctgatcgct gccgatccgg cagcgtggtc agccatggcg atgctgctgt
     1801 ccgcggccta ttttgcggtg ccggaaaccg gtgcccgcca cggctacttc ggccagcaac
     1861 cccgtccggc acagcccgac cgctatccag cctatatcgc cgagggcctg atcgaccaca
     1921 tgctcgtgcc gggttggcgc gaacggtggg cggcgggcga tccggtcgcc gcctgatgcg
     1981 ggagggaatg cccggaccgt gaagtgaagc cttgcgccgg ggagcctatg gggcatggta
     2041 tggccccgga cgacgtccac ttccgcagcc tctgcgacgc agaccgcggg gtgcaggccg
     2101 ccccgtttga gaaggagcac aatcatggcc tccgcatctt taagcctcgc gccagggatg
     2161 agccccggcg aatggcagac ccgcgtcgag cttgccgcgt gctaccgcct catcgcgctt
     2221 cacggctggg acgatcttat cgcgacgcac atttccgccc gggttccggg cacccgcgat
     2281 ttcctcatca accgcctcgg gctgaccttc gaagagatca ccgcgtcaaa tctcgtgcgc
     2341 atcgatctgg acggcaatgt caccgacggc tcggacgccc cgatcaatcg cgcaggcttc
     2401 accattcaca gcgcggtgca tcaggtccgc gaggacgcgg gatgcgtgat tcatctccac
     2461 acgctcgatg ggacagccgt gtcggcgctg gaggacggac tgctccccct caaccagatt
     2521 gcgatgctgg cccatggcga catcgccttc cacgaatacg aaggcgtggc cttcgatctt
     2581 gccgagcgtg agcggctcca ggccgacctc gggcagagca acctgatgct gctgcgcaat
     2641 catggcacgc tggccgttgg ccggaccgtg ggcgaagcct atacccgcat gtacacgctc
     2701 gaacgcgcct gctccatcca ggtgcggacg ctggggatga accggccgct ccatgccgct
     2761 gacgccgggg tgatcgaacg gacgggagac ctgggccgcg atgcggcgag ctatgcggcc
     2821 gtcgcctggc cggcggcgct gcgccgggcg gggcggctca gccccgggtt cgacagctga
     2881 accatggccg atcgctccgc gatcgtggtc gccagccagt tcggcgacga ggctaacgcg
     2941 gcgattgcct ccctggtgcc gggcgtccgc atccggcctg ttccgctcgg ctaccaggcg
     3001 cccgatatcc tcgacgccga cgtgttcctg cacatcccgg acgttcgcac ggaacgcggc
     3061 ggctcgagcg agcgaccggc gggttggccg gggcggctgc ggctggtcca ggtaatttcg
     3121 accggcacgg acttctaccc cgactggctg tttgccggcc ccgcggtcac gacggcccag
     3181 gggctcgccg ggccggcgat ctccgagtat tgcctcgcgg cgatctttgc tgccgcgcgg
     3241 gacatgcccc gcatctggat ggctggcccg caggactggc gacggcttca gtgccagcag
     3301 gtctccggca gcacgctggg gctgttcggc tttggcagta tcggcagtac gctggcccga
     3361 aaggcgcttg cccttggcat gcgggtggcg gccgtccgcc gctcgcggga gcccctgggc
     3421 atcgacggcg tggagcagct ggaccagctg ggggccttgc tggcccgctg cgatcatgtg
     3481 gtcctcgccg cgccgggcac ggccgagacg cgtcacgtgg tcaatcgcga aaccctggcc
     3541 atggcgcgcc ccggcctgca tctcgtcaat gtcgcccgcg gcgggctgat cgacgacaat
     3601 gccttactgg cttcactgga caacggcacg gtcgggcgcg cgacgctcga cgtaaccgag
     3661 cccgagccgc tgcctgccgg ccatcggttc tatacccacc cgcgcgtgtt cctcagcccc
     3721 cacatcgcca attcggggcc ggccttcctc gcggcgatca gccgccgcgc cgccgacaac
     3781 atcaagcgcc tggaagcagg cggcgagctt gtgggagtgt cagcggcgcg cgtctgaact
     3841 atgccgcgtt cgccggcgcc gctgcggtag cgccagcctt gatcgaatcg gcagctcgcc
     3901 agccggagaa cacgcagtca gccagggaca ggccgctgac gtagaagttg gagcagacgc
     3961 cgatggcggt ccgccccgcg gcatagagcc ccggcaccgg agccccggca tcgtcaagca
     4021 cggccccgct gtcctcattg accttgagcc cgcccatggt gatgcccatg atcggattca
     4081 tctttgaatc gtacgacatg ttgtaggcat agaagggccc cgtttcgatc agccgccgat
     4141 agctgtcggc cttgccccag gcgtccggct gcccggcgcg gatcgccgcg ttgtagcttt
     4201 cgaccgttcg caccagagca tcggcgggta cgccgatctt ggcggcgagt tcgggcaggg
     4261 tgttcgcgcg ggccaggccg aattgcatct gggcgcgcaa ctgcatcttc atgtagggct
     4321 ggagttcggg atcctggcgc tgttcctgca ccagatcgtg cagcggctga tcgccgatca
     4381 cccagccgcg cccgccgctc ttcctgaaca gcgcgtcacc ggttcgcgca ccgtacagtt
     4441 cctcgttgac gatccgctcg cccgcggtgc cgacgacgac acccgttgtc caggcaaccg
     4501 gcggatagag gaagcgccag gcggagcagt cacccagctt gtcggtcgcg ccgccgacgc
     4561 tggcgccgag cttgatgccc gaaccatcgt cgccgaaggt gcccagcggg gccatgccca
     4621 tgaactcggg agccaggcgc tgcatcatcg tgcgattgaa cgcgaaccca cccgccgaca
     4681 ggactaccgc ccggcgcacg cgcacgcgcg tgagcttggc atttttgcgc tcgaggcgg
//
LOCUS       DATNBV010000302         3387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4313085,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000302 DATNBV010000000
VERSION     DATNBV010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4313085"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1293
                     /gene="hemW"
                     /locus_tag="VN627_07280"
     CDS             142..1293
                     /gene="hemW"
                     /locus_tag="VN627_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HXD03095.1"
                     /translation="MARALYIHWPFCLKKCPYCDFNSHVRDGVDHDLWREALLTDLRH
                     EAQITANEPLESIFFGGGTPSLMPPALVEALLKEAERLWGFAPDIEITLEANPSSVEA
                     SKFADLAQAGVNRVSLGLQALDDEALRFLGRLHNVDESLKALATAQEHFQRVTFDLIY
                     ARPEHTPESWHAELTRALAFGTGHLSLYQLTIEAGTRFATMVRQGQFTPLDDDLAADL
                     FTLTRELTAKAGLPAYEISNHARAHEESRHNLAYWRYHDYAGIGPGAHGRRGGMATVR
                     HRKPENWLEAVAATGHGLSEERPLGRSEQASEALLMGLRLAEGVDLAALSARFGLTEP
                     ELIDARGLAGPLRQGLVWREGSRLGVTEAGMPVLDALLGELVAASLLAA"
     gene            1290..2198
                     /locus_tag="VN627_07285"
     CDS             1290..2198
                     /locus_tag="VN627_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HXD03096.1"
                     /translation="MSTPLAARAALLEAWHNHLAQNRRRSPHTVRAYVATAARLVDAL
                     QLQDWPAVARLDAADLRRQLAARRADGIGNVSAARELSALKGFLAFARAKTGEANRAP
                     PRLRGPRIKKGLPRPVTPDEAVHLMEMVGDLAADDWIGSRNRAVLLLLYGAGLRIAEA
                     LSLTAAALPLGETLVVTGKGGKQRMVPLLPVVRAGVADYVMKCPWPLDKASPLFRGAK
                     GGALSQGLVQKAMAQARVALGLPPGATPHALRHSFATHLLGAGADLRSLQELLGHASL
                     GSTQIYTRVDAATLLDVYRSAHPREQ"
     gene            complement(2360..2959)
                     /locus_tag="VN627_07290"
     CDS             complement(2360..2959)
                     /locus_tag="VN627_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXD03097.1"
                     /translation="MNDWIIHLIEAGGYWGIGFLMALENIFPPIPSELIMGLGGVAVA
                     RGSMTFWPLLLVGTVGSTLGNYCWFLLGDRFGYARLRPFVARWGRWLTMEWEDVEAAT
                     GFFRRHGQWVVFAMRFSPFMRTLVSLPAGLAHMGHGRFLLFTFAGAAVWNTLLIHGGR
                     LVARYLAGFEGALGWGLLALMAGFVGWYLWRVARWTPRG"
     gene            complement(2994..>3387)
                     /locus_tag="VN627_07295"
     CDS             complement(2994..>3387)
                     /locus_tag="VN627_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HXD03098.1"
                     /translation="AWVEPFMVQPFLPGVAKGDKRIVLVDGVVAGAINRRPGAGEFRS
                     NLAVGGSAEATTLTAREEEICAAMGPVLKERGLVFVGIDVIGGEWLTEINVTSPTGIV
                     AIDRFNGSDTGGMIWDAIEARHGAMLKG"
BASE COUNT          572 a         1374 c          977 g          464 t
ORIGIN      
        1 cctgaacttg tttcaggatc caaaggccga cctatccccc atcgcggcgg tagaaccgga
       61 agcgccgtgc ctgcggcgca gttggcgcca tggatgctga atcaggttca gcatgacgag
      121 cagtggtgtt agtgcccatc catggcccga gccctctaca tccactggcc gttttgcctg
      181 aagaagtgcc catactgcga cttcaacagc cacgtccgcg atggcgtcga ccatgacctc
      241 tggcgcgagg ccctgctgac ggacctgcgc cacgaagccc agataaccgc aaacgaaccg
      301 ctcgaatcca tattcttcgg cggcggcacc ccctcgctga tgcccccggc gttggtcgaa
      361 gccctgctca aggaggccga acgcctgtgg ggtttcgccc ccgacatcga gatcaccctc
      421 gaagccaacc cctcctcggt cgaagccagc aagttcgccg atctcgcgca ggccggcgtt
      481 aaccgcgtca gtctcggcct ccaggcgctg gacgatgaag ccctgcgctt ccttggccgc
      541 ctccacaacg tcgacgaaag cctgaaagcc ctcgccaccg cgcaagaaca cttccagcgc
      601 gtcaccttcg acctgatcta cgcccgcccc gaacacaccc ccgaaagctg gcatgccgaa
      661 ctcacccgcg ccctcgcctt cggcaccggc cacctttcgc tctaccagct caccatcgaa
      721 gccggcaccc gctttgccac catggtccgg caaggccagt tcaccccgct tgacgacgat
      781 ctcgccgccg acctgttcac cctcacccgc gagctgaccg ccaaggccgg gctccccgcc
      841 tacgagatca gcaaccacgc gcgggcccac gaggaaagcc gccacaacct cgcctactgg
      901 cgctaccatg actatgccgg catcggcccc ggcgcccacg gccgccgggg cggcatggcc
      961 acggtccgcc accgcaaacc ggaaaactgg ctggaagccg tcgccgccac cggccacggc
     1021 ctttcggagg agcgcccgct cggccgctcc gaacaggcca gcgaagccct gctcatgggc
     1081 ctgcgcctcg ccgaaggggt agacctcgcc gccctctccg cgcgcttcgg tctcaccgaa
     1141 ccggaactga tcgacgccag gggcctcgcc ggcccgctgc ggcaaggcct cgtctggcgc
     1201 gaaggcagtc gccttggcgt caccgaggcc ggcatgcccg tgctcgatgc gctgcttggc
     1261 gaactggttg ccgccagtct cctggccgca tgagcactcc tctcgccgcc cgcgccgccc
     1321 tgctcgaagc ctggcacaac cacctggcgc aaaaccgccg ccgctcgccc cacactgtgc
     1381 gcgcctatgt cgccacggcc gcccggctgg tcgatgcgct gcaactgcag gactggcccg
     1441 ccgtggcccg gctcgatgcc gccgacctgc gccgccagct cgccgcccgc cgcgccgacg
     1501 gcatcggcaa tgtctccgcc gcgcgcgaac tctccgcgct caagggcttc ctcgccttcg
     1561 cccgcgccaa gactggcgaa gccaaccgcg ccccgccccg gctgcgcggc ccgcgcatca
     1621 agaagggcct cccccgcccg gtcacgccgg acgaggcggt ccacctgatg gaaatggtcg
     1681 gcgatctcgc cgcggacgac tggatcggct cacgcaaccg cgccgtcctc ctcctgctct
     1741 acggcgccgg cctgcgcatc gccgaggccc tctcgctcac cgccgccgcc ttgccgctgg
     1801 gcgagacgct ggtcgtcacc ggcaagggcg gcaagcagcg catggtcccg ctcctccccg
     1861 tcgtccgcgc cggcgtggcc gactatgtca tgaaatgccc gtggccgctg gacaaggcat
     1921 ccccgctgtt ccgcggggcc aagggcggcg ccctgtcgca gggcctcgtc cagaaggcga
     1981 tggcccaggc ccgcgtcgcg ctgggcctgc cgcccggcgc caccccccac gccctgcgcc
     2041 attccttcgc cacccacctc ctcggcgccg gggcggacct gcgcagcttg caggaactgc
     2101 tcggccacgc cagcctcggc tcaacccaga tctacaccag ggtcgacgcc gccaccctgc
     2161 tggacgtcta ccgcagcgcc cacccgcgcg agcagtaggg caggtctcgg ctccgcctca
     2221 caagtgatgg gtgcacatcg ccagcaagag gatcagcggg atcggcacac ccacggccca
     2281 gagcaagacc gaacgcatgg cattctctcc taagctattg tattcaggag agaatcccgc
     2341 aggcgccggt tgcgttccct cagccccgcg gggtccaccg cgccacgcgc cacagatacc
     2401 agccgacaaa ccccgccatc agcgccagca ggccccaacc tagcgcaccc tcgaacccgg
     2461 caagatagcg cgccaccagc cgcccgccgt ggatcagcag cgtgttccac accgccgccc
     2521 cggcgaaagt gaacagcagg aaccgcccgt gccccatgtg cgccagcccc gccggcagcg
     2581 agaccagcgt gcgcatgaac ggcgagaagc gcattgcgaa gaccacccac tggccatgcc
     2641 gccggaagaa gcccgtcgcg gcctccacgt cctcccactc catcgtcagc caccgccccc
     2701 accgcgcgac aaacggccgc aggcgggcat agccgaaccg gtcgcccagc aggaaccagc
     2761 agtagttgcc cagcgtcgag ccgacggtcc ccaccagcag caacggccag aacgtcatcg
     2821 acccgcgcgc cacggccacg ccgcccagcc ccatgatcag ctcggagggg atcggcggaa
     2881 agatgttctc cagcgccatc aggaacccga tgccccaata gccgccagcc tcgatgaggt
     2941 ggatgatcca gtcgttcatg tgtgtgcaac gggccgcgcg gagataggag ccgtcacccc
     3001 ttcagcatcg ccccatgccg cgcctcgatc gcgtcccaga tcatcccgcc ggtgtcgctg
     3061 ccgttgaacc ggtcgatcgc cacgatcccg gtcgggctgg tcacgttgat ctcggtcagc
     3121 cactcgcccc cgatcacgtc gatgccgacg aagaccagcc ctcgctcttt cagcaccggg
     3181 cccatggcgg cgcagatttc ctcctcccgc gccgtcagcg tcgtcgcctc ggccgaaccg
     3241 ccaacggcca ggttgctccg gaattccccc gcccccggcc gccggttgat cgcgccggcc
     3301 accacgccgt ccaccagcac gatccgcttg tcgcccttgg ccacgcccgg caagaacggc
     3361 tggaccatga acggctcgac ccaggca
//
LOCUS       DATNBV010000303         4626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2735601,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000303 DATNBV010000000
VERSION     DATNBV010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2735601"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VN627_07300"
     CDS             <1..405
                     /locus_tag="VN627_07300"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF014586.4"
                     /note="NH(3)-dependent; catalyzes the formation of
                     nicotinamide adenine dinucleotide (NAD) from nicotinic
                     acid adenine dinucleotide (NAAD) using either ammonia or
                     glutamine as the amide donor and ATP; ammonia-utilizing
                     enzymes include the ones from Bacillus and Escherichia
                     coli while glutamine-utilizing enzymes include the
                     Mycobacterial one; forms homodimers; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HXD03099.1"
                     /translation="LIGDLYKTEVFALSRHLNADAGRELIPAAIIDKEPSAELAPDQR
                     DSDSLPPYPVLDCILKVLIEGPRLGPAERAEVEADYASLSADPEGQALVAKVRRMIAR
                     NEYKRRQAPPILRLRGRAFGSGRQMPIAAKYD"
     gene            416..1444
                     /locus_tag="VN627_07305"
     CDS             416..1444
                     /locus_tag="VN627_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamide-nucleotide
                     adenylyltransferase/Nudix hydroxylase"
                     /protein_id="HXD03100.1"
                     /translation="MNSSGTYDVAVLIGRFQPFHNGHAALLAEAVDSAPQVIVVLGSA
                     HAARNAKNPFTWQERAAMIAATLDEATRQRVRFVPLRDYYDDRRWGAAVVAVVKAHCT
                     PAARVALVGYLKDASSHYLQRFPGWELVAAPQFGEIDATAIRRIYFDGEDWAATEALL
                     GGLLPPAIRHYLKGWAQLPPFAQLRDEHLALEDNKRTWGSGPFVTVDAVVTAADHVLL
                     VERGQAPGKGLWALPGGFLEPRERLLQGAIRELREETGLALLDSTLEAALTAVAVFDH
                     PDRSLRGRTITHAHRFELGQCRLPEVAGADDAAAARWVPIAELAGMEGVFFEDHFNIL
                     DHFLGLAD"
     gene            complement(1441..1767)
                     /locus_tag="VN627_07310"
     CDS             complement(1441..1767)
                     /locus_tag="VN627_07310"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HXD03101.1"
                     /translation="MTFDASLWLIFILIALATTLPRASFIVLGNRVALPSVLQRALRY
                     APAAALAAIVVPDVLVVGGAVEVFNPKLAAAIAAVAAALAWRNPWLPFIAGMAVLLGL
                     RFALGS"
     gene            complement(1764..2540)
                     /locus_tag="VN627_07315"
     CDS             complement(1764..2540)
                     /locus_tag="VN627_07315"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HXD03102.1"
                     /translation="MASRSWKASSRALDYAAVISENSPLPSSFRSGAREGFFAFLPLS
                     VGLVPWALVMGMAMASTGFSPLQAMGMNIIVFAGTAQLGTLPLIAAEAPLWLIVTTAL
                     ALNLRFVIFSAALAPGFRGVGTPARWLAGHLLTDGVFATCLDKMMSVEDPHWRLGYYL
                     APAIWSWLLWQSFALIGIYAAGFIPKQWSLEFMATIALIVLLVPMARIRPMLVAALVG
                     GASATLLRDIPLRLGVVVGICAGIAAGFAAEHWAHRSKRP"
     gene            2594..3127
                     /locus_tag="VN627_07320"
     CDS             2594..3127
                     /locus_tag="VN627_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03103.1"
                     /translation="MGNPSMIKLAGLLVTLCLFLSGCAGYAGSRLQPGVSGRTEVLAE
                     MGEPAMHWKNGDGSEQLAYPRGPQGTHTYMVFIGPDGRLQRIESVLDVSHFARIVAGK
                     SDQAAVLRLLGPPVPDWTVYFKARDELVWEWLFCDDFSRLARFNVLFDGTTGLVRTTM
                     QRPDLRGWDGIAPFCGR"
     gene            3174..>4626
                     /gene="glpK"
                     /locus_tag="VN627_07325"
     CDS             3174..>4626
                     /gene="glpK"
                     /locus_tag="VN627_07325"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HXD03104.1"
                     /translation="MSATADRNSRYVLALDQGTTSSRAILFDRQGQLCGVSQQPFAQS
                     YPQPGWVEQDAEEIWQSQLTVARTVLRENGVTAGQVAAIGIANQRETTVLWDRRTGQA
                     LAPAIVWQDRRTASQCAALAAAGHGELFEQRTGLCLDPYFSGTKLAWLLDHIPGARQR
                     AEKGELAFGTVDSWLAWKLTGGRAHVTDPSNTSRTLLFDIRRGVWDDELLARLAIPAS
                     LLPRVVDSSGVMASIDAEWLGGEIPLAGLAGDQQAATFGQVCLTPGMAKNTYGTGCFL
                     LMNAGRQAMPSRHRLLSTVGWRYGGQTTYLLEGSVFMGGAVVQWLRDGLGMVASAAEI
                     EPLAAAVPDSGGVYLVPAHTGLGAPYWDPHARGALLGLTRGSGRAQIARAALEAIAFQ
                     SAEVLIAMEQDAGHPLAELRVDGGAAANDLLLQIQADLLGVPVVRPTITETTALGAAY
                     LAGLAVGFWHDEAELSALWQVDRRFEPGRSADWR"
BASE COUNT          752 a         1588 c         1621 g          665 t
ORIGIN      
        1 ctgatcggcg acctctacaa gacggaagtc ttcgccctca gccgccacct caatgccgat
       61 gccgggcgcg agctgatccc ggcggccatc atcgacaagg agccctcggc cgaactggcg
      121 cccgaccagc gcgacagcga cagcctgcca ccttaccctg tgctcgactg catcctcaag
      181 gtgctgatcg aaggcccgcg cctcggccct gccgaacggg ccgaagtcga agctgactac
      241 gccagcctga gcgccgaccc ggaagggcag gccctggtcg ccaaggtccg gcggatgatc
      301 gcccgcaacg aatacaagcg gcgccaggcg ccgcccatcc tgcgcctgcg cggccgggcc
      361 ttcggcagcg ggcggcagat gccgatcgcc gcgaaatacg actgagggag aagtcatgaa
      421 cagcagcgga acttacgatg tcgcggtcct catcggccgc ttccagcctt tccacaacgg
      481 ccatgccgcc ttgctcgccg aggccgtgga cagtgcgccg caggtcatcg tcgtgctcgg
      541 ctcggcgcat gcggcgcgca atgccaagaa ccccttcacc tggcaggagc gcgccgcgat
      601 gatcgccgcg acgctcgacg aagcgacccg gcagcgcgtg cgcttcgtgc cgctgcgcga
      661 ctattacgac gaccggcgct ggggggcggc cgtcgtggcg gtggtcaagg cacactgcac
      721 cccggccgcg agggtcgccc tggtcggcta tctgaaggat gcctccagcc actatctgca
      781 gcgctttccc ggctgggaac tggtcgccgc gccgcaattc ggcgagatcg acgccaccgc
      841 catccgccgc atctatttcg acggcgagga ctgggcggcg accgaggccc tgctcggcgg
      901 tctgctgccg ccggccatca ggcattacct caagggctgg gcgcagctgc cgccgttcgc
      961 gcaactgcgc gacgaacacc tggccctcga agacaacaag cggacctggg gcagcggccc
     1021 cttcgtcacg gtcgatgccg tagtcacggc ggccgaccac gtgctgctcg tcgaacgcgg
     1081 gcaggccccg ggcaaagggc tgtgggcgct gcccggcggc ttcctcgagc cgcgcgagcg
     1141 gcttctgcaa ggggcgatcc gcgaattgcg cgaggagacc gggctggccc tgctcgactc
     1201 gacgctggaa gccgcgctga ccgcggtcgc cgtcttcgat cacccggacc gtagcctgcg
     1261 cggccggacc attacccatg cccaccgctt cgagctcggg cagtgccggc tgccggaagt
     1321 cgccggcgcc gacgatgcgg cggcggcgcg ctgggtgccg attgccgaat tggccgggat
     1381 ggaaggagtg ttcttcgagg accatttcaa catcctcgat cacttccttg ggctggccga
     1441 ctaactgccc agcgcgaagc gcaggccgag cagcaccgcc atgccggcga tgaagggcag
     1501 ccaggggttg cgccaggcca gcgcggcggc gacggcggcg atcgccgcgg cgagcttggg
     1561 gttgaagact tcgaccgcgc cgccgacgac cagcacgtcg ggcacgacga tcgccgccag
     1621 ggcggcggcc ggggcgtagc gcagggcgcg ctggagcacc gagggcaggg cgacgcggtt
     1681 gccgagcacg atgaagctgg cgcgcggcag cgtcgtggcc agcgcgatca ggatgaagat
     1741 cagccacagc gaggcgtcga aagtcatggc cgtttactcc ggtgtgccca gtgttcggcc
     1801 gcaaagccgg cggcgatgcc ggcgcagatg ccgaccacga cgccgaggcg caacgggatg
     1861 tcgcgcagca gcgtcgcgct ggcgccgccg accagcgccg cgaccagcat cgggcggatg
     1921 cgggccatcg gcaccaggag cacgatcagg gcgatggtcg ccatgaattc gagcgaccat
     1981 tgtttcggga tgaagccggc ggcgtagatg ccgatcagcg caaagctctg ccacagcagc
     2041 caggaccaga tggccggcgc caggtagtag ccgagccgcc agtgcgggtc ctcgacgctc
     2101 atcatcttgt cgaggcaggt cgcgaacacg ccgtcggtca gcaggtggcc ggccagccag
     2161 cgggccggcg tgccgacgcc gcgaaagccg ggggcgaggg cggcgctgaa gatcacgaag
     2221 cgcaggttca gggccagcgc cgtggtgacg atcagccaga gcggggcctc ggcggcgatc
     2281 agcggcaggg tgccgagctg ggcggtgccg gcgaagacga tgatgttcat gcccatcgcc
     2341 tgcaagggcg aaaagccggt gctggccatc gccatcccca tgaccagcgc ccagggcacg
     2401 aggccgaccg agagcggcag gaaggcgaag aagccctcgc gggcaccgga acggaagctg
     2461 gaaggcaagg ggctgttctc gctgatgacg gccgcgtagt ctagcgcccg ggagctagcc
     2521 ttccaagacc ggctcgccat ctgccgccga gcggcgcaca attggcggaa aagggggcag
     2581 caaggagcga atcatgggga atccgtcgat gatcaaactg gccgggctgc tcgttacgct
     2641 ctgcctgttc ctgagcggct gcgccggcta tgccggaagc cggctccagc cgggcgtttc
     2701 cggccgcacc gaggtgctgg ccgaaatggg cgaaccggcc atgcactgga agaacggtga
     2761 cggcagcgag cagctggcct atccgcgtgg tccgcagggc acgcatacct atatggtgtt
     2821 cattggaccc gacggccgcc tgcagaggat cgagagcgtc ctcgatgtat cccatttcgc
     2881 ccgcatcgtg gcggggaaga gcgaccaggc ggcggtgttg cgcctgctcg gcccaccggt
     2941 gccggactgg acggtctatt tcaaggcgcg cgacgaactg gtctgggagt ggctgttctg
     3001 cgacgatttc agccggctgg cccgcttcaa cgtgttgttc gacggcacga cggggctggt
     3061 ccggacgacc atgcagcgcc cggacctgcg tggctgggac ggcatcgcgc ccttctgcgg
     3121 acgctagcgc cagcctttga atttatggtg gaatgaacat tcagggggat gccatgagcg
     3181 ccaccgccga ccgtaacagc cgctatgtcc tggctctcga ccagggcacg accagttcgc
     3241 gggccatcct ctttgaccgc caggggcaac tctgcggcgt cagtcagcag cccttcgccc
     3301 agtcctatcc gcaacccggc tgggtcgagc aggatgccga ggaaatctgg cagagccagc
     3361 tcaccgtggc gcgcaccgtc ctgcgcgaga atggcgtgac ggccgggcag gtcgccgcca
     3421 tcggtatcgc caaccagcgc gaaaccacgg tgctctggga tcgccggacg gggcaggcgt
     3481 tggccccggc catcgtctgg caggatcggc ggaccgcctc gcaatgtgcg gcgctggccg
     3541 ccgccggcca cggcgagttg ttcgaacagc gcaccggctt gtgcctcgac ccatattttt
     3601 ccggtaccaa gctggcctgg ctgctcgacc atattcccgg cgcccgccag cgggcggaaa
     3661 aaggcgaact cgctttcggc accgtcgaca gctggctggc ctggaaactg accggcgggc
     3721 gggcccacgt caccgacccc tcgaatacct cgcgcaccct gctcttcgac atccggcgcg
     3781 gcgtctggga cgacgagctg ctcgcccggt tggccattcc cgccagcctg ttgccgcgcg
     3841 tcgtcgacag cagcggggtg atggccagca tcgatgccga atggctgggc ggggaaattc
     3901 cgctcgccgg cctggccggc gaccagcagg cggcgacttt cgggcaggtt tgcctgacgc
     3961 ccggcatggc caagaatacc tacggcacgg gctgcttcct gctgatgaat gccggccgcc
     4021 aggccatgcc ctcgcgccac cggttgttga gtacggtcgg ctggcgctat ggcggccaga
     4081 ccacctacct gctcgaaggc agcgtcttca tgggcggcgc cgtcgtccag tggctgcgcg
     4141 acggcctggg catggtcgcc agcgctgccg aaatcgagcc gctcgccgcc gcggtgccgg
     4201 acagcggcgg cgtctatctg gtgccggccc acaccgggct cggcgcgccc tactgggatc
     4261 cgcatgcccg cggcgccctg ctcggcctga cccggggcag cggccgcgcc cagatcgccc
     4321 gcgccgcgct cgaggccatc gccttccaga gcgccgaagt gctgatcgcc atggaacagg
     4381 acgccggtca cccgctggcc gaactgcgcg tcgatggcgg ggcggcggcc aacgacctgc
     4441 tgctccagat ccaggccgac ctgctcggtg tgccagtcgt ccgcccgacc atcaccgaaa
     4501 cgacggcgct cggcgccgcc tacctggccg gtctggcggt cggcttctgg cacgacgaag
     4561 ccgaactgag cgccctgtgg caggtcgatc gccgcttcga gccgggtcgt tcggccgact
     4621 ggcggg
//
LOCUS       DATNBV010000304         6196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4649703,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000304 DATNBV010000000
VERSION     DATNBV010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4649703"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VN627_07330"
     CDS             <1..368
                     /locus_tag="VN627_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXD03105.1"
                     /translation="DVFAALAAQPRPASAYDVAEGLSARLGRRVAANTVYRILDLFVR
                     TNLARRVESANAYIANPHPGCRHDCIFLICDSCGVAVHSDDDALTGALIGAARKAGFA
                     DVRPVIELRGTCARCSGES"
     gene            427..2349
                     /gene="dxs"
                     /locus_tag="VN627_07335"
     CDS             427..2349
                     /gene="dxs"
                     /locus_tag="VN627_07335"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HXD03106.1"
                     /translation="MTAAPATPLLDTVAYPADLRRLAPGQLRQLADELRTEMISAVGQ
                     TGGHLGSGLGVVELTVALHYVFNTPDDRLVWDVGHQAYPHKILTGRRDRIRTLRQPGG
                     LSGFTKRTESEYDPFGTAHSSTSISAALGFAVANKLAGGDGKGIAVIGDGAMSAGMAY
                     EAMNNAKAAGNRLVVILNDNDMSIAPPVGGLSAYLARLVSSRQFMGLRGLARRISRHL
                     PEPLHKAARKTDEFARGMAMGGTLFEELGFYYVGPVDGHNLDHLIPVLENVRDAAEGP
                     ILVHVVTQKGKGYAPAEAAADKYHGVQKFDVITGQQVKAAAGPPAYQNVFGETLAKLA
                     DSDPKICAITAAMPSGTGVDRFAKAHPERSFDVGIAEQHAVTFAAGLAAQGMRPFCAI
                     YSTFLQRAYDQVVHDVAIQNLPVRFAIDRAGLVGADGATHAGSFDITYLASLPNMVVM
                     AAADEAELVHMTYTASLHDSGPIAFRYPRGNGTGVALPEVPQQLEIGKGRIVREGTKI
                     ALLSLGTRLAEALKAADQLEARGLSTTVADLRFAKPLDEELIRRLATTHEVVVTVEEG
                     AIGGLGAHILTLASDEGLVDTGLKIRTMRLPDAFQDHDSPDRQYDAAGLNAPQIVDTV
                     LKALRHNSAGVEEARA"
     gene            complement(2359..3045)
                     /locus_tag="VN627_07340"
     CDS             complement(2359..3045)
                     /locus_tag="VN627_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HXD03107.1"
                     /translation="MGADHAELTGAAVAFVGSHMLLSGPLRRPLRRLLGERGFLLAYS
                     LVAFATLGTMIWAFDRAEPGEPLWDGTALVPWIIASALTVIALALFLGSFNGNPALPQ
                     ANLAGLSARKAWGAFTITRHPMMMAFALWALAHMLVAPTLRTLILAGAVMVLALAGSH
                     QQDRRKRAEARREWETWMARTSFWPRWRALPALGVWWPVAILLWLAVTWAHIPLGHVP
                     AGLWRWVNTG"
     gene            complement(3106..4611)
                     /locus_tag="VN627_07345"
     CDS             complement(3106..4611)
                     /locus_tag="VN627_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HXD03108.1"
                     /translation="MLATRRSREAMGHPRGLFVLFYAEMWERFSYYGMRALLVLYLTK
                     FWLFADGRANLIYGSYGSLVYITPVLGGYVADRWLGQRKAVVFGGVLLALGHLFMAVE
                     GLRGVTDPMLKQADPAINVFWLALALIIVGSGFLKANISVIVGQLYGLADTRRDAAYT
                     IFYMGVNAGAAFGTILVGYLGETIGWAWGFGLAGIGMVLGLAIFLTGRAALEGRGEPP
                     EPLAKGTEWRIYGVGLVAVALAWGLIQYQSVIGNLLAVSGVAMLGYTLYVASKLPPEP
                     RQRIYAILFLVAVNPIFWGLFEQAGGSLNLYTDKFVDRQGVPTSLFQSINPIYIILFA
                     PAFAALWQWLGRRGLEPSAPAKFALALVQGGLSFLIFVWGARTVGAEAVTPVLFVFLI
                     YLLQTTGELCLSPVGLSAMTRLAPAHLGSFIMGAWFYTTALGQFVAGRIGEATGGESG
                     EMSKALTLAIYEKIGWITLGVAVVVFLFSRIVTRWMHLGTLGEVDREGGLA"
     gene            5003..5284
                     /locus_tag="VN627_07350"
     CDS             5003..5284
                     /locus_tag="VN627_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HXD03109.1"
                     /translation="MIRSELLAALATENPGLRAEEVERVVDVFFDEIAARLAAGGRVE
                     LRGFGAFSTREREPRKGRNPRTGASVEVPGKRVPYFKPGKEMRQRLNAD"
     gene            5419..5503
                     /locus_tag="VN627_07355"
     tRNA            5419..5503
                     /locus_tag="VN627_07355"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5452..5454,aa:Leu,seq:taa)
     gene            complement(5507..>6196)
                     /locus_tag="VN627_07360"
     CDS             complement(5507..>6196)
                     /locus_tag="VN627_07360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXD03110.1"
                     /translation="MALDLDGAAVATDGSGAFLIAFDRDAPAEARLRARAANGWESAL
                     PLAIAPRAWQIERIDAPLRPPTVPSEEFMARRKAELAQIAAARALVTPAQGWAQEMIR
                     PAPGRISGRFGAQRIYRGEPGAYHTGLDLAASAGTPILCPADGVVILAANAPFTLEGK
                     LLMVDHGMGLSSAFLHCSAHRVSLGEAVKRGQPVAEVGMTGRATGPHLHWGLRWRQAR
                     LDPLLFLPQEA"
BASE COUNT         1098 a         2193 c         2025 g          880 t
ORIGIN      
        1 ccgacgtctt cgccgccctc gccgcccagc cgcgtccggc ctcggcctac gacgtggccg
       61 agggcctgtc ggcccggctc ggccggcggg tcgccgccaa cacggtctac cgcatcctcg
      121 atctgttcgt gcgcaccaac ctcgcccgcc gggtggagag cgccaacgcc tatatcgcca
      181 acccccaccc aggctgccgg cacgactgca tctttctcat ctgcgattcc tgcggggtcg
      241 ccgtccacag cgacgacgat gcgttgaccg gggccctgat cggcgcggcc cgcaaggccg
      301 gtttcgccga cgtgcgtccg gtcatcgagc tgcgcggcac ctgcgcgcgc tgctcgggcg
      361 aaagctgaga cgagtgaccc atcgatacaa tcgacttggg tcccgccagc gtgtagggcc
      421 gatgggatga ctgccgctcc ggccaccccc ctgctcgaca ctgtcgccta tcccgccgac
      481 ctcaggcgcc tggcccccgg gcagctgcgc cagctggccg atgagctgcg caccgaaatg
      541 atcagcgccg tggggcagac ggggggacac ctcggctcgg gtctcggcgt ggtcgaactc
      601 acggtcgcgc tgcactacgt gttcaacacg ccggacgacc ggctggtgtg ggacgtcggc
      661 caccaggcct atccccacaa gatcctcacc ggccggcgtg accggatccg caccctgcgc
      721 cagccgggcg gcctgtccgg cttcaccaag cgcacggaga gcgaatacga tcccttcggg
      781 accgcccaca gctccacctc gatttccgcg gcgctgggct ttgccgtggc caacaagctg
      841 gctggtggcg atggcaaggg catcgccgtg atcggcgacg gcgcgatgag cgcgggcatg
      901 gcctatgagg cgatgaacaa cgccaaggcc gccggcaacc ggctcgtcgt catcctcaac
      961 gacaacgaca tgtcgatcgc cccgccggtc ggcgggcttt cggcctatct cgcgcggctg
     1021 gtctcctcac gccagttcat gggcctgcgc ggcctcgccc gccgcatctc gcgccacttg
     1081 cccgagccgc tccacaaggc cgcccgcaag accgacgagt tcgcccgcgg catggccatg
     1141 ggcggcaccc tgtttgagga acttggcttc tactatgtcg gcccggttga cgggcacaat
     1201 ctcgaccacc tgatcccggt gctggaaaac gtgcgcgatg cggccgaagg gccgatcctc
     1261 gtccacgtgg tcacgcaaaa gggcaagggc tatgccccgg ccgaagccgc ggccgacaag
     1321 tatcacggcg tccagaaatt cgacgtgatc accggccagc aggtcaaggc cgccgcgggc
     1381 ccgccggcct accagaacgt cttcggcgaa acgctggcca agctggccga cagcgatccg
     1441 aaaatctgcg cgatcaccgc cgccatgccg tcgggcaccg gggtggacag gttcgccaag
     1501 gcccacccgg agcgcagctt cgacgtgggt atcgccgaac agcatgccgt caccttcgcc
     1561 gccggcctcg ccgcgcaagg gatgcgcccg ttctgcgcga tctattcgac cttcctccag
     1621 cgcgcctacg accaggtcgt tcacgacgtg gcgatccaga acctgccggt gcgcttcgcg
     1681 atcgaccgcg ccggcctcgt cggcgccgac ggggcgactc acgccggctc tttcgacatc
     1741 acctatctcg ccagcctgcc caacatggtg gtcatggccg cggccgacga ggccgagctg
     1801 gtccacatga cctacaccgc cagcctgcac gacagcggcc cgatcgcctt ccgctatccg
     1861 cgcggcaacg gcactggcgt cgccttgccg gaggtgcccc agcagctgga gatcggcaag
     1921 ggccggatcg tgcgcgaagg caccaagatt gcgttgcttt cgctcggcac gcggctggcc
     1981 gaggcgctca aggcggccga ccagctcgag gcgcgcgggc tctccaccac cgtggccgat
     2041 ctgcgcttcg ccaagccgct ggacgaggag ctgatccgcc gcctcgccac cacccacgaa
     2101 gtcgtggtca ccgtcgagga aggcgccatc ggcggtctcg gcgcccacat cctgaccctg
     2161 gcgagcgacg aagggctggt ggacaccggc ctcaagatcc gcacgatgcg cctgcctgac
     2221 gcgttccagg accatgacag cccggacagg cagtatgacg cggccgggct caatgcgccg
     2281 cagatcgtgg ataccgtgct caaggccctg cgccacaaca gcgcgggcgt cgaggaagcg
     2341 cgcgcctagg gtcgggacct agccggtatt cacccaccgc cacagcccgg ccggaacatg
     2401 gccgagcggg atgtgcgccc aggtcaccgc cagccacagc agtattgcca ccggccacca
     2461 cacgcccagc gccggcaggg cgcgccagcg cggccagaag ctggtgcggg ccatccaggt
     2521 ttcccattcc cggcgcgcct cggcgcgctt gcgccggtcc tgctggtgcg atcccgccag
     2581 ggcaaggacc atcacggcac cggcaagaat cagggtccgc agcgtggggg cgaccagcat
     2641 gtgggccagg gcccagaggg cgaaagccat catcatcgga tggcgggtaa tggtgaaggc
     2701 cccccacgcc ttgcgcgccg agagaccggc gaggttggcc tgcggcaggg ccggattgcc
     2761 gttgaacgag cccaggaaca gggcgagggc gatgacagtc agcgcagagg cgatgatcca
     2821 cggcaccagg gccgtgccgt cccacagcgg ttcgcccggc tcagcgcggt cgaacgccca
     2881 gatcatggtg cccagcgtgg cgaaggccac cagcgagtag gccagcagga agccgcgctc
     2941 acccaacagg cgccgcagcg ggcgccgcaa tggaccggac agcagcatat ggctgccgac
     3001 aaatgcgacg gccgcgccgg tcagctcggc atgatcagcc cccattgccc ccattcctct
     3061 cgaaagttag cccctttttc cgggcgctta tcagcctttt gaaggttatg ccaagccccc
     3121 ttcgcgatcg acctcgccca gcgtaccgag atgcatccat cgcgtcacga tgcgcgagaa
     3181 caggaagacg accaccgcca cgcccagcgt aatccagccg atcttctcgt agatcgcaag
     3241 ggtcagcgcc ttgctcattt cgccgctctc cccgccggtg gcttcgccga tccggccggc
     3301 gacgaactgg ccgagcgcgg tcgtgtagaa ccaggcgccc atgatgaacg agccgaggtg
     3361 ggcaggcgca aggcgcgtca tggccgagag gccgaccggc gagaggcaca gctcgcccgt
     3421 ggtctgcagc aggtagatca ggaacacgaa caggacaggt gtcaccgcct cggcgccaac
     3481 cgtgcgcgcg ccccagacaa atatgaggaa cgagagcccg ccctgcacca gcgccagggc
     3541 gaacttggcc ggcgcgctcg gctcgagccc gcgccggccg agccattgcc acagggcggc
     3601 gaaggctggg gcgaacagga tgatatagat cgggttgatc gactggaaca ggctggtggg
     3661 cacgccctgt cggtcgacga acttgtcggt atagaggttg aggctgcctc cggcctgctc
     3721 gaacaggccc cagaagatcg ggttcaccgc caccaggaac aggatcgcgt agatccgctg
     3781 gcgcggctcg ggcggcagct tcgaggcgac gtagagcgtg tagccgagca tggccacgcc
     3841 gctgacggcc agcagattgc caatgaccga ctggtactgg atcagccccc aggccagagc
     3901 caccgccacc agtcccacgc cgtagatccg ccactccgtg cccttggcca gcggctcggg
     3961 cggttcgccc cggccttcca gcgcggcccg cccagtgagg aagatggcga ggcccagcac
     4021 catgccgatg ccggccagcc cgaagcccca ggcccaaccg atggtctcgc caaggtagcc
     4081 caccaggatc gtgccgaagg cggcgcccgc gttcaccccc atgtagaaga tcgtataggc
     4141 cgcatcccgc cgggtgtcgg ccagaccata gagctggccg acgatgaccg agatattggc
     4201 cttgagaaag cccgagccga cgataatcag cgccagcgcc agccagaaca cgttgatcgc
     4261 cgggtccgcc tgcttgagca tcggatcggt taccccgcgc aggccctcaa cggccatgaa
     4321 caggtggccc agcgccagca gcaccccgcc gaagaccacc gccttgcgct ggcccagcca
     4381 gcggtccgcc acatagcccc ccagcaccgg ggtgatgtag acgaggctgc cgtaggagcc
     4441 gtagatcagg ttggccctgc cgtcggcgaa cagccagaac ttggtgaggt agagcaccag
     4501 cagcgcgcgc atgccgtagt aggagaaccg ctcccacatc tcggcataga acagcacgaa
     4561 cagcccgcgc gggtggccca tggcctcgcg cgaccggcgg gtggcaagca tgccgccgat
     4621 ggccaggaag gcgaacagcg ccagtgcccc gatcgccgcg atccagtcac cctcggccca
     4681 ttacgccact gccttcatcg ccatccagcc ctcgcgtgag atatgtcagg cgggcaacct
     4741 aggctctaat gcgcgggtga aaagccaaaa tgagcgcgcc aatactgtcc cgaatcgaac
     4801 aaagatggtt ttgcaatcga tgatcaacac agattgcgcg gagccgtaac ttctggcaaa
     4861 aagccaatag ttgtcggcac cgcctgcccg aagggacatc tggcgtgagc ggtagcaggg
     4921 tcgatggggg tcgatctgcc ggcaatttaa cgatgggatc ggcgctgggc agcacgtctg
     4981 gttgcccgac gggagacatt gaatgattcg ctctgaattg ctcgccgccc ttgcgacgga
     5041 gaatccggga ctgcgcgccg aagaagtcga acgagtagtg gacgtgttct ttgatgagat
     5101 cgccgcgcgc ctcgctgccg gcggccgggt ggaattgcgg ggcttcggcg ccttttccac
     5161 gcgggagcgc gaaccgcgca agggccgcaa cccgcgtacc ggcgcctcgg tcgaagtgcc
     5221 gggcaagcgc gtgccctact tcaagcccgg caaggaaatg cgccagcgcc tgaacgccga
     5281 ttgatcggag cggcgaaaga ccatccggct ttcgcccttc gcgcaaacac ccttcccgca
     5341 agccccgcga tctgccatcc gccatttggg cgggtgccat tttgctcggg gctcgctggg
     5401 cgaaaaggcg cccgccatgc ggacgtggcg gaatggtaga cgcaggggac ttaaaatccc
     5461 ttgagcatag ctcgtgcggg ttcgagtccc gccgtccgta ccaggatcag gcttcctgcg
     5521 gcaggaacag cagcgggtcg agccgggcct ggcgccagcg caggccccag tgcaagtgcg
     5581 ggcccgtggc gcggccggtc attcccactt cggcaacagg ctggccccgc ttcaccgcct
     5641 cgcccaagct cacgcggtgg gccgagcagt gcaggaaagc actcgacagt cccatgccgt
     5701 ggtccaccat cagcagcttg ccctccagcg tgaacggcgc gttggcggcg aggatgacca
     5761 ccccgtcggc tgggcacagg atcggggtgc cggcgctcgc cgccaggtcc agcccggtat
     5821 gataggcgcc gggttcgccc cggtagatcc gctgcgcccc gaaccgcccc gatatccgcc
     5881 ccggggcagg acgaatcatc tcctgggccc agccctgagc cggcgtaacc agggcccggg
     5941 cggcggcgat ctgcgccagt tcggccttgc ggcgggccat gaattcctcg ctcggcaccg
     6001 tcggcgggcg cagcggtgca tcgatccgct cgatctgcca ggcgcgcgga gcgatggcga
     6061 gcggcagggc cgattcccag ccattggccg cccgcgcacg gaggcgggcc tctgccggcg
     6121 catcgcgatc aaaggcgatc aggaaagcgc ccgagccgtc agtcgccacg gccgcgccat
     6181 cgaggtcgag cgccat
//
LOCUS       DATNBV010000305         2264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_191755,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000305 DATNBV010000000
VERSION     DATNBV010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191755"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VN627_07365"
     CDS             complement(<1..405)
                     /locus_tag="VN627_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXD03111.1"
                     /translation="MDNRINYRRAARALLLGAAALATPPAVADDLRDALASAYRTNPT
                     LQGARAQQRAVDEGVPIARAQGLPSLGGTANYSETLKASENNRILGTLVGPDRVLDLA
                     ATASYPVFSGGAVRNAINAAETRVRAGQSDLRG"
     gene            complement(436..1044)
                     /locus_tag="VN627_07370"
     CDS             complement(436..1044)
                     /locus_tag="VN627_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HXD03112.1"
                     /translation="MTLVEESVESPVTQSIAARRAMIDSQLRVSGVNDPAILAAMDAV
                     AREQFVPAALGANAYIDRALPLGDGHALPAPLVTGRMLTEAEVAPGQAVLVVSPAGYL
                     AAVATAMGAVVTALAPAEAAAAKKGGEVSLILIDGAIEQLPTGLAARLAEGGRVVTGL
                     VERGVTRLAVGRKTAGEVALLPLAEIGMPVLAEFAAPKRWSF"
     gene            1359..>2264
                     /locus_tag="VN627_07375"
     CDS             1359..>2264
                     /locus_tag="VN627_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HXD03113.1"
                     /translation="MSDMVVIREEDLIESVADALQYISYFHPMDYIRALGDAYEAEVG
                     PAAKDAIAQILTNSRMCAEGHRPICQDTGIVNVFVEWGQDCRLESDRSLQEVVDEGVR
                     RAYLNADNKLRASVLADPAFTRRNTKDNTPSVLHVTMVKGNKVSVDVAAKGGGSENKT
                     KFKMMNPSENIVDWVIDMLPQMGAGWCPPGMLGIGIGGTAEHCMLLAKQALMEPIDMA
                     QLKQRGPQNKLEALRIEIFDKVNALGIGAQGLGGLSTILDVKILDWPTHAASKPVAMI
                     PNCAATRHAHFELDGSGPAFLEPPSL"
BASE COUNT          377 a          771 c          774 g          342 t
ORIGIN      
        1 gccccgcaga tccgactggc cggcccggac acgggtttcg gcggcgttga tcgcgttgcg
       61 cacggcgccg cctgagaaca ccggatagga ggccgtggcg gcaagatcga gcacgcgatc
      121 cgggccaacc agggtcccaa ggatgcggtt gttttcgctc gccttcagag tctcgctgta
      181 gttggccgtg ccaccgagcg aaggcagtcc ctgggcacgg gcaatcggca cgccttcatc
      241 gactgcgcgc tgctgggcgc gggcgccctg cagggtgggg ttggtgcgat aggccgaggc
      301 gagggcatcg cgcagatcgt cagccacggc cggcggggtg gcgagcgcgg cggcgccgag
      361 caggagggcc cttgccgccc ttcggtagtt aattcgattg tccatgactt ggcacgcccc
      421 cccgagcatg gttgatcaga agctccagcg cttcggcgcg gcaaattcgg caaggaccgg
      481 catgccgatc tcggccagcg gcagcagggc cacttcgccc gcggtcttgc ggccgacggc
      541 gaggcgcgtg acgccgcgtt ccaccaggcc ggtcacgacg cggccgcctt cggcgaggcg
      601 cgcagcgagg ccggtgggca gttgttcgat ggccccatcg atcaggatca gcgagacttc
      661 gccgcccttc ttcgcggccg cggcttcggc tggggccagg gcggttacca cggcgcccat
      721 ggccgtggcg acagcggcga ggtagccggc cggcgagacc accagcacgg cctggcccgg
      781 ggccacttcc gcctcggtca gcatgcggcc ggtgaccagc ggagcgggca gggcatggcc
      841 atcacccagc ggcagggcgc gatcgatata ggcgttggca ccgagggcgg cgggcacgaa
      901 ctgttcgcgc gcgaccgcat ccatcgcggc gagaatggcg ggatcgttga ccccgctgac
      961 gcgcaactgg ctgtcgatca ttgcgcggcg cgcggcgatg gactgggtta ccgggctttc
     1021 cacgctttct tccacaaggg tcatgctcaa accattcctg cgcgggcccg cgagcgggcc
     1081 gcgaaaaacc tgcctttggc gcggcgccgt tgctgccaac ggacccggcc atccgccgcg
     1141 ccttctaggc caaggccccc ggcccgccaa gcgctttcgc ggctgcggca tccgcgcgca
     1201 tgccggaatg cgcgcgaggg cctatccccg atggcggcgc cgaggcccct tgcccgacct
     1261 cctcgctgca acgccgcatc tcacgcttta cctgcccgcg cgattgggcc tatgggccct
     1321 gcccatatcc catcagctgc atgcaaaggg ccgccatcat gtccgatatg gtcgttattc
     1381 gcgaggaaga cctgatcgag agcgtcgccg acgccctgca gtacatcagc tatttccacc
     1441 cgatggacta catccgcgcg ctgggcgatg cctatgaggc ggaagtcggc ccggcggcca
     1501 aggacgcgat cgcgcagatc ctcaccaaca gccggatgtg cgccgaagga caccggccga
     1561 tctgccagga caccggcatc gtcaacgtct tcgtcgagtg gggccaggac tgccggctgg
     1621 agagcgaccg gtcgttgcag gaagtcgtcg atgaaggcgt acgccgcgcc tatctcaacg
     1681 ccgacaacaa gctgcgcgcc tcggtcctgg ccgacccggc ctttacccgc cgcaacacca
     1741 aggacaacac gccgagcgtg ctccacgtca cgatggtcaa gggcaacaag gtttcggtcg
     1801 acgtcgcggc caagggcggc gggtcggaga acaagaccaa gttcaagatg atgaacccga
     1861 gcgagaacat cgttgactgg gtgatcgaca tgctaccgca gatgggcgcg ggctggtgcc
     1921 cgccgggcat gctcggcatc ggcatcggcg gcacggccga acactgcatg ctgctggcca
     1981 agcaggcgtt gatggagccg atcgacatgg cccagctcaa gcagcgcggc ccgcagaaca
     2041 agctcgaggc gctgcgcatc gagatcttcg acaaggtcaa cgcgctcggc atcggcgcgc
     2101 aggggctggg tggcctgtcg accatcctcg acgtcaagat cctcgactgg ccgacccatg
     2161 cggcatccaa gccggtggcg atgatcccga attgcgcggc gacgcgccat gcccatttcg
     2221 agctcgacgg ttcgggcccg gcctttctcg agccgccgag cctc
//
LOCUS       DATNBV010000306         2052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1459965,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000306 DATNBV010000000
VERSION     DATNBV010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459965"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1664
                     /locus_tag="VN627_07380"
     CDS             <1..1664
                     /locus_tag="VN627_07380"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXD03114.1"
                     /translation="TAIPESELDMWIEETGLDPLARGLEWKHEPAPQQKENFFVVVIG
                     CGISGINAAVHLKAAGIPFIVIEKNPGIGGVWFENRYPGIRVDSPSRGYLHLFGLEYP
                     QPYAFCPGEENVRYGEWVLDQFDLRSHLVLDTEVSDLEWDDEAQVWNIRGQGKDGPQT
                     WRANAVFSCVGFLSRPFKPVIPGLEDFAGTVCHTAEWPKGLDLAGKRVGVIGSGASGY
                     QTVPVIAKVAAQTTLFQRNASWCYATPGYLQRLPEEVLWGERNIPYYVNFARFRMGRM
                     YGPDNTLISQRIDADFKDPHARSAANKAMRDNCVAFIEQSLAGRPDLIEKMTPDIPPM
                     TSRPIRIDPEDNVYLSLLQDNCELVTDPIERVTPKGILVGGREIELDVIVLATGFRAN
                     DFLWPMEVRGRDGFTAQELWEKDGPRAYIGSMLPGFPNLFMSYGPNTNNFGGLQVIDL
                     LEMEVRFGLQCIAGLIEQGKHSVEVQADAYWRFNDELDRIERQMIYADPRVTSYYRNE
                     HGRSSVNGPIDIRRMWRWLRDPAGTPQEKTDAGLRPWFGEDLIVR"
     gene            1757..>2052
                     /locus_tag="VN627_07385"
     CDS             1757..>2052
                     /locus_tag="VN627_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03115.1"
                     /translation="MAIHSRYQIGGRRTGRAASAELAQVRAGSADAPSPLPHSPAARS
                     ERAAIGGIDCLVVTPDIAATGDMVYCHGGGYRRGSPYFMSGFLSQIAARTGVRI"
BASE COUNT          360 a          661 c          672 g          359 t
ORIGIN      
        1 ggaccgcgat cccggaatcc gagctggaca tgtggatcga ggagaccggc ctcgatcccc
       61 tggcgcgcgg cctggagtgg aagcacgagc cggcaccgca gcagaaggaa aacttcttcg
      121 tcgtggtgat cggctgcggt atttccggga tcaatgcggc agtccacctc aaagccgccg
      181 gcattccgtt catcgtgatc gagaagaacc ccgggatcgg cggggtctgg ttcgaaaacc
      241 gctatcccgg gatccgggtc gattccccca gccgcggcta tctccacctg ttcggccttg
      301 agtacccgca gccctatgcc ttctgcccgg gcgaggagaa cgtgcgctac ggcgaatggg
      361 ttctcgacca gttcgacctg cgctcccatc tcgtgctgga tactgaggtg tccgatctgg
      421 aatgggacga tgaggcccag gtctggaaca tccgcgggca gggcaaggac ggtccgcaaa
      481 cctggcgggc caatgccgta ttctcttgcg tcggcttcct gtcccgcccg ttcaagccgg
      541 tcattcccgg ccttgaagac ttcgccggca ccgtctgcca cacggcggaa tggcccaagg
      601 ggctcgatct cgcgggcaag cgcgtcgggg tgatcggttc gggcgcatcg ggctaccaga
      661 ccgtgccggt gattgccaag gtcgccgcgc aaaccacctt gttccagcgc aacgccagct
      721 ggtgctatgc cacgccaggc tatctgcagc ggttgccgga agaggtgctg tggggcgagc
      781 gcaacatccc ctactacgtc aacttcgcgc ggtttcggat gggccggatg tacgggccgg
      841 acaacaccct catcagccag cgcatcgatg ccgatttcaa ggacccgcat gcgcgcagcg
      901 cggccaacaa ggccatgcgt gacaactgcg tggccttcat cgagcaatcg ctcgccgggc
      961 ggcccgacct gatcgagaag atgacccccg acatcccgcc gatgacctcc cggcccatcc
     1021 ggatcgaccc cgaggacaat gtctacctgt cgctgctgca ggacaattgc gagctggtta
     1081 ccgacccgat cgagcgggtc acgccgaagg gcatcctggt gggcggccgg gagatcgagc
     1141 tcgatgtcat agtgttggcc accggcttca gggccaacga tttcctctgg ccgatggagg
     1201 tccgcgggcg cgacggcttc accgcgcagg agctctggga gaaggatggg ccgcgcgcct
     1261 acatcggatc gatgctgccg ggcttcccga acctgttcat gtcctacggg cccaacacca
     1321 acaacttcgg cgggctccag gtgatcgacc tgctcgagat ggaggtgcgg ttcgggctcc
     1381 agtgcatcgc cgggctgatc gagcagggca agcacagcgt cgaggttcag gccgatgcct
     1441 actggcgctt caatgatgag ctcgaccgga tcgagcgcca gatgatctat gccgacccgc
     1501 gtgtgacgag ctattaccgc aacgaacatg gccgttcgag cgtcaacggg ccgatcgaca
     1561 tccgccgcat gtggcgctgg ctgcgcgatc ccgccggcac gccgcaggag aagaccgacg
     1621 ccggtctgcg gccctggttc ggggaagacc tgatcgtgcg ctgaacctcg atcagccgga
     1681 attagcgtat tgcggctgtt cggctattca ggacgaagcg gttgattcga ccggctgcgg
     1741 caaggttgac tagggcatgg ccatccattc gcgttaccaa atcggcggcc ggcgaaccgg
     1801 tcgcgccgcg tctgcggaac tggcgcaggt ccgcgccggg tcggccgacg cgccgtcgcc
     1861 gttgccgcac agcccggcgg cgcggtccga acgtgcggcc attggcggga tagactgcct
     1921 ggtcgtgacg cccgacatcg ctgcaaccgg cgatatggtc tattgccacg gtggcggtta
     1981 caggcgcggc tcgccctatt tcatgagcgg atttctcagc cagatcgccg cccgcaccgg
     2041 cgtgaggatc at
//
LOCUS       DATNBV010000307         5904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3379104,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000307 DATNBV010000000
VERSION     DATNBV010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3379104"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..563
                     /gene="ybeY"
                     /locus_tag="VN627_07390"
     CDS             78..563
                     /gene="ybeY"
                     /locus_tag="VN627_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXD03116.1"
                     /translation="MSLEIDIEEPWPIEADWADLAEAAAEAAMAVAPELDNQRLSASV
                     LFTSDAEVHVLNREWRAKDKPTNVLSFPMITRADLIAMGKDGAPEMLGDLALAYETCA
                     REAADKGITVEAHAAHLIVHGLLHLAGHDHELGEAEAGAMEALETKALAQMGIADPYR
                     T"
     gene            609..1520
                     /locus_tag="VN627_07395"
     CDS             609..1520
                     /locus_tag="VN627_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXD03117.1"
                     /translation="MADHGRNGESAGGEADSRRSLWKGLRRLLGGDHDQSLRAALEEA
                     IDEHEDEGDAPEGDLSQLERQMVRNLLHFSEHDADDVAIPRGEIVAVPTSASWDELVA
                     MFAEHGHSRMPVYRDTLDEIVGMMHLKDVFPFLATAKAPPRDWTVLMRQPLFVPQARG
                     ALDVLADMRASRVHLAVVIDEYSGTDGIITIEDLVEEIVGEIEDEHDDAPTDLLVALG
                     DGTWEADARAELDDVGERIDPRLAEVEEDIDTIGGLAFLLAGQVPPIGAVLAHSSGWN
                     LEVTAGNERHVTRLRLHPPGETQPAPA"
     gene            1561..2439
                     /locus_tag="VN627_07400"
     CDS             1561..2439
                     /locus_tag="VN627_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXD03118.1"
                     /translation="MRRLPPLRALEAFVRVVRLGSAKAAATELGLSPSALSRRVQALE
                     DFTGKKLFHRQHQAMRLTEDGQAFYDAVSPKLDELADAVESLVDTGQVLRLHLGVPPL
                     FGGQRLFPRLPELRKLHPRLHIDIDTSPHQETRVGDSLDAAIILSKGPDASLYSVRLD
                     HNRVYAITSRAIADELGPEPDLAKLSKQTFLIHHDLPDSFVAWKAATGIGDFTPVAVD
                     QYDSGQLLLEAAANGLGIAIMHDDHFARSGDERLARLYDFEVESPYSYWFVCKPRALE
                     TRPVRLFHDWLVKAGL"
     gene            complement(2815..3288)
                     /gene="ybaK"
                     /locus_tag="VN627_07405"
     CDS             complement(2815..3288)
                     /gene="ybaK"
                     /locus_tag="VN627_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HXD03119.1"
                     /translation="MAKGTPATLVLVRSGIAFTLHEYAYDPAADRVGLQAAEAMGVDP
                     GCVFKTLLVLADGKPACAIVASDRQVAMKQLAAALGSKSAAMMPPDQAERLTGYKVGG
                     ISPFGQRRQVPTVIDEAALAHDAIFLNGGRRGLQVRMAPGDAVQVIGAKVAAIAA"
     gene            complement(3359..3829)
                     /locus_tag="VN627_07410"
     CDS             complement(3359..3829)
                     /locus_tag="VN627_07410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXD03120.1"
                     /translation="MADEFLTLTLDTGNGQGGDVKIKLRPDLAPGHVARIKELVGEGF
                     YDGIKFHRVIPGFMAQGGCPNGTGMGGSSKPDLQAEFNAEPHVRGVCSMARTSAPHSA
                     NSQFFICFDDATFLDRQYTVWGQVVEGMEHVDALPKGEPPREPGKIVKATVSEG"
     gene            3941..5401
                     /gene="mgtE"
                     /locus_tag="VN627_07415"
     CDS             3941..5401
                     /gene="mgtE"
                     /locus_tag="VN627_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXD03121.1"
                     /translation="MAADDRAPDLLEQADALSGTEDLREAPERTEDSDRLDKENRLKS
                     DFVRRVRDALEEGERDQVYDLVEPLHPADIADLFELLAPDERPGLAAAITDLLSADVI
                     AELNDHVREALVEDLDAEDIAEIAEQLDTDDAVAMLEDLDEEDQQAVLAEMEPEDRAA
                     IESALAFPEESAGRIMSRDFIAVPEYMSVGDLIDYLRRDKDLATEFWEVFVVDQRHHP
                     VGTCALSWILRAPRDVALADVMKRDQTLIPVGMDQEEVALRFQKYALISAAVVDDSGR
                     LVGQITVDDVVHIIQEEAGEDALLLSGAGDGDINEPLPSTIKTRMWWLTINLGTAILA
                     SAVVAAFESTISRLVTLAVLMPIVTGMGGNAATQTMTVTVRALATNQLTSSNTWRMIM
                     REWWIASANGWGLGILMAIGCQLVYHDWWLSMVLLLAMLLNSLNAGLSGVLIPMGLNR
                     LKIDPAVTSTVFVTTMTDTFGFFFFLGLADLFKLSA"
     gene            5411..5803
                     /locus_tag="VN627_07420"
     CDS             5411..5803
                     /locus_tag="VN627_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HXD03122.1"
                     /translation="MPLSMTKVAYGAQSLEEVHGWFEQRGEVHRVTTRYLPKRHAEMI
                     GGSVYWILKHQLVARSEILGFEETEGGRTAIVVSGRLIDVRPRPRRAHQGWRYLEESD
                     CPGDLAAGEAGEVLPGHLAHELGKLGLL"
BASE COUNT          998 a         1954 c         1990 g          962 t
ORIGIN      
        1 agaggccggg gttctggcat agccttgacc ggacgccctt gctcttgatc cgtggctccc
       61 ctagccatcg gttcatgctg tcgcttgaaa tcgatatcga ggaaccctgg ccgatcgagg
      121 cggattgggc ggatctggcc gaggctgccg ccgaggcggc gatggccgtc gccccggagc
      181 tggacaacca gcggctctcc gcctcagtgc tgttcaccag tgacgccgag gtccacgtgc
      241 tgaaccgcga gtggcgggcc aaggacaagc cgaccaacgt gctttccttc ccgatgatca
      301 cgcgggccga cctcatcgcc atgggcaagg acggcgcccc ggaaatgctg ggcgatctgg
      361 ccctggctta cgagacttgc gcccgcgagg ccgccgacaa gggcatcacc gtggaggccc
      421 atgccgccca cctgatcgtc cacggcctgc tccatctggc cgggcacgac cacgaactgg
      481 gcgaagccga agccggggcg atggaggcgc tcgaaacaaa ggcgcttgcg caaatgggga
      541 tcgccgaccc atatcgcaca tgaaggcgcc gcggacggcg cggggctgat acaaggatat
      601 cgaggcagat ggccgatcat ggccgcaatg gcgagagcgc cggcggagag gcggacagta
      661 ggcgcagcct gtggaagggc ctgcgtcgtc tgctcggcgg cgatcatgac cagtcgctgc
      721 gcgcggccct cgaagaggcg atcgacgagc acgaggacga gggggacgcg ccggagggcg
      781 atctttcgca gctcgaacgg cagatggtcc gcaacctgct ccacttcagc gagcacgatg
      841 ccgacgacgt ggcgatcccg cgcggcgaaa tcgtcgccgt gccgacttcc gcaagctggg
      901 acgagctcgt cgccatgttc gccgagcatg gccacagccg catgccggtc taccgcgaca
      961 cgctcgacga gatcgtcggc atgatgcacc tcaaggacgt gtttccgttc cttgccaccg
     1021 ccaaggcccc gccgcgcgac tggaccgtgc tgatgcgcca gccgctgttc gtgccgcagg
     1081 cgcgcggcgc gctcgacgtg ctggccgaca tgcgggccag ccgggtccac cttgccgtgg
     1141 tcatcgacga gtattccggg accgacggga tcatcaccat cgaggatctg gtcgaggaga
     1201 tcgtcggcga gatcgaggac gagcatgacg acgcgcccac ggacctgctg gttgccctgg
     1261 gcgacggaac ctgggaggcc gatgcccggg ccgagctcga cgatgtgggc gagcggattg
     1321 acccgcgcct ggccgaggtc gaggaggata tcgacaccat tgggggcctg gccttcctcc
     1381 tggccggaca ggttccgccc attggggcag ttcttgctca ttccagtggc tggaatcttg
     1441 aggtaactgc gggtaacgaa cgccacgtta cccggttgcg actgcatcca cctggcgaaa
     1501 cccagccggc cccggcctga cgaccaacca gaaccaaaac agaaagaccg tcttctgccc
     1561 atgcgccgat tgccgcccct aagagcgctt gaagcctttg tgcgggtcgt tcgccttggc
     1621 tcggccaagg ccgcggcgac cgagctgggc ctgagtcctt cggccctgtc gcgccgggtc
     1681 caggcgctgg aggacttcac cggcaagaag ctgttccacc gccagcatca ggccatgcgc
     1741 ctgaccgagg acgggcaggc gttctacgat gccgtcagcc ccaagctcga cgaactggcc
     1801 gatgcggtgg aaagtctggt cgataccggg caggtcctgc gcctgcacct gggcgtgccg
     1861 ccgctgttcg gcgggcagcg gctgttcccc cgccttcccg aactgcgcaa gctccacccg
     1921 cgcctgcaca tcgacatcga taccagcccg caccaggaaa cccgggtcgg cgattcgctc
     1981 gatgccgcga tcatcctgtc gaaggggccg gacgcgtcgc tctattcggt gcggctcgac
     2041 cacaaccggg tctatgcgat cacctcaagg gcgatcgcgg acgaacttgg gccggagccc
     2101 gatctcgcca agctttcgaa gcagaccttc ctcatccatc acgatctgcc cgacagcttc
     2161 gtggcgtgga aggcggctac cggcatcggc gatttcacgc cggtggcggt ggaccagtac
     2221 gattcgggcc agctcctgct cgaagcggca gccaatggcc tgggcatcgc gatcatgcac
     2281 gacgaccatt tcgcccgctc gggcgacgag cggctggccc ggctctatga cttcgaggtg
     2341 gagagcccct attcctactg gttcgtgtgc aagccgcgcg cgctggaaac ccgcccggtg
     2401 cggctgtttc acgactggct ggtgaaggcg gggctctagg ggcgtttccc tagccgcggc
     2461 ttggcgaaga tttggcgacc ggcgcggcac aagcggggca tgaccctgat cgaccagtcg
     2521 ctgttcaccc caaccccgca tttccgcgcc cgtcgccgcc gggtcatggt ccgcgtgcgg
     2581 gtggcaggcg gcgatggccc ggcgcgcgag gccatggcgc gcgacgtttc gcctgcgggc
     2641 ttgagcgcca tcgcgcaggt ggcggcaccc gggctcggga ggtggtcacc gtggccctgc
     2701 cagacggctc gaccctgtgg ggcgtggtgc gctgggtgga gggcaaggct ttcggcgtgg
     2761 agttcgatac cggttcgcgc gatgggccgg ttaccgcgat cccgggttga ccgttcaggc
     2821 ggcgatcgcg gccaccttgg cgccgatcac ctgaaccgca tcccccggcg ccatacgcac
     2881 ctgcaggccg cggcggcctc cattgagaaa tatggcgtcg tgggccagcg cggcttcatc
     2941 gatgacggtc ggcacctggc gccgctgacc gaaggggctg atgccgccaa ccttgtagcc
     3001 ggtcagccgc tctgcctgat cgggcggcat catcgccgcg gacttggagc ccagggccgc
     3061 cgccagttgc ttcatcgcca cctgacggtc ggacgcgaca atcgcgcagg cgggctttcc
     3121 atcggcgagg accagcagcg tcttgaacac gcagcccgga tcgacgccca tggcctcggc
     3181 cgcttgcagg ccgacgcggt ctgccgccgg atcataggca tattcgtgaa gcgtgaaggc
     3241 gatcccggac cggaccagta ccagcgtggc gggcgtgccc ttggccatgc gcggttcctc
     3301 aagaatcagg gcgcggaaat tgcttcccgc gcccccttga tcgttagcga agagccggtc
     3361 agccttccga gacggtggcc ttgacgatct tgcccggctc gcgcgggggt tcgcccttgg
     3421 gcagcgcatc gacatgctcc atgccttcga ccacctggcc ccagacggtg tactggcgat
     3481 cgaggaaggt cgcatcatcg aagcagatga agaactggct gttggccgag tgcggcgccg
     3541 aagtgcgggc catcgagcag acgccgcgca cgtggggttc ggcgttgaat tcggcctgga
     3601 gatcgggctt cgacgaaccg cccatgccgg tgccattggg gcagccgccc tgggccatga
     3661 agccggggat cacccggtgg aacttgattc catcatagaa gccttcaccg acgagttcct
     3721 tgatgcgggc gacgtggccc ggggcgagat cggggcgcag cttgatcttg acgtcgccgc
     3781 cctggccgtt gccggtatcg agcgtgaggg tgaggaattc atcggccatc tggcggtctc
     3841 cttgagaata gctcgcgccg atataggctg ctcgaacata aagtcacggg ggacccgccg
     3901 ggccggttgc aattatggaa ttgggcaata gaccggccgc atggctgccg acgaccgcgc
     3961 ccccgatctg ctcgagcagg ccgacgccct ttccgggacc gaggacctgc gcgaagcgcc
     4021 agagcggacc gaggattcgg accggctgga caaggaaaac cggctgaaga gcgacttcgt
     4081 ccgccgggtc cgcgacgcgc tcgaagaggg cgagcgggac caggtctatg acctggtgga
     4141 gccgctgcat ccggccgaca tcgccgacct gttcgaactg ctcgcccccg acgagcgccc
     4201 gggcctcgcc gcggcgatca ccgacctgct cagcgccgac gtcatcgccg aactcaatga
     4261 ccacgtccgc gaggccctgg tcgaggacct cgacgccgag gacatcgccg agatcgccga
     4321 gcagctcgat accgacgacg cggtcgccat gctcgaggat ctcgacgagg aggaccagca
     4381 ggccgtcctt gccgagatgg agccggagga ccgcgcggcg atcgagagcg cgctggcctt
     4441 ccccgaggaa tccgccggcc gcatcatgag ccgcgatttc atcgcggtgc ccgaatacat
     4501 gagcgtgggc gacctgatcg actacctgcg ccgcgacaag gacctggcga ccgaattctg
     4561 ggaagtcttc gtggtcgacc agcgccacca cccggtcggc acctgcgcgc tctcgtggat
     4621 tctgcgagcc ccccgtgacg tggccctggc cgacgtgatg aagcgcgacc agacgctgat
     4681 cccggtcggc atggaccagg aagaagtggc gctgcgcttc cagaaatatg cgctgatctc
     4741 ggccgcggtg gttgacgatt cgggccgtct ggtcggccag atcacggtcg acgatgtggt
     4801 tcacatcatc caggaagagg ccggcgagga cgccctgctg ctctcgggcg cgggcgacgg
     4861 cgacatcaac gagccgctcc ccagcacgat caagacccgc atgtggtggc tgaccatcaa
     4921 cctcggcacg gcgatcctcg cctcggccgt ggtcgccgcc ttcgagagca ccatcagccg
     4981 gctggtcacg ctcgccgtgc tgatgccgat cgtcaccggc atgggcggca atgccgcgac
     5041 ccagaccatg acggtgacgg tgcgcgccct cgccaccaat cagctgacca gttccaacac
     5101 ctggcgcatg atcatgcgcg aatggtggat cgcctcggcc aatggctggg gcctgggcat
     5161 tctcatggcg atcggctgtc agctggtcta ccacgactgg tggctgagca tggttctgct
     5221 gctggccatg ctgctcaaca gcctcaatgc cggcctatcg ggcgtgctga ttccaatggg
     5281 gctcaaccgg ctgaagatcg acccggcggt gacctccacc gtcttcgtga cgacgatgac
     5341 cgatacgttc ggattcttct tcttcctggg cctggccgac ctgttcaagc tgagcgcttg
     5401 agccggggcc gtgccgctca gcatgaccaa ggtggcctat ggggcgcagt cgctggagga
     5461 agtccacggc tggttcgagc agcgcggtga ggtccaccgg gtgacgacgc gctacctgcc
     5521 caagcgccat gccgagatga tcggcggctc ggtctactgg atcctcaagc accagctggt
     5581 ggcgcgttcg gaaatcctcg gcttcgagga aaccgagggc gggcgcacgg ccatcgtggt
     5641 ttccggacgg ctgatcgacg ttcgcccacg cccccgccgc gcgcaccagg gatggcgcta
     5701 tctcgaagag agcgattgcc ccggcgacct ggccgctggt gaagcgggcg aagtcctgcc
     5761 cggccatctc gcccatgaac tgggcaagct cggcctgctc taagccgggg cttggcccag
     5821 gccggggcga gccctaaagt ttggcaatga ccgacctcgc gccccggctg aagatcaagc
     5881 tacaagtcta ttgcggcgag gaaa
//
LOCUS       DATNBV010000308         2575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4651903,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000308 DATNBV010000000
VERSION     DATNBV010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4651903"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VN627_07425"
     CDS             <1..1056
                     /locus_tag="VN627_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03123.1"
                     /translation="TLRAPDTPRPETTALHRELAEPGALTPAARGIALAVVGLSWAWA
                     IGTIAMFMISMLAPAHPEAPLPSVGDFTRAYGPWIILATVGVPLVTAAVTNWLKAHRR
                     SVLTAPDTARSSLRTAANHLYVALVSPLAELSARLGWGVLVVIGMILTYTLCYNIWSS
                     FAFPFYLDFLKYSKDEVAFASKVFGIFMTMFGISLGGILFARLGRMPTILIGAVLPIL
                     GNFVYADLAEGAPLIDAFGSAIGLKALFGLFGFDERMIRLLLAISFENVSTGIAGAAF
                     VAYLSGIVSKSYTAVQYALLSSLTFLIGSLGRGIAGEAFDQFGYATVFRWTAAAGLAA
                     VLFVLLEWARVRRAERA"
     gene            complement(1133..>2575)
                     /locus_tag="VN627_07430"
     CDS             complement(1133..>2575)
                     /locus_tag="VN627_07430"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HXD03124.1"
                     /translation="GGGPRAVIRYAQEMEEPPVPLHHLRWSPSPFRGGMMRDFATSDD
                     PRVQLQLDRLALLSLPQGRFGLETITQLCARLGNPERRLPPVFHVAGTNGKGSTCAFL
                     RAVLEAQGYTVHTATKPHLVRYNERIRIAGRLIADAALAEVLAEVLDAGEDLGPSFFE
                     VTTAALFLAFSRTPADACVIEVGLGGRFDATNVIPAPLVCGIAALGIDHEAFLLRPEE
                     GVPAEPLARIAFEKASIAKPGVPLVTQAYPALAADEVVRTAVAHGAVLLMRGRDWWAD
                     MGIEGIAFRDGRGELALPLPALPGAHQGDNAALAVAMLRAQSALPISDEAIARGLREA
                     RWPARLQRLGDGPLTALVPGREVWLDGGHNADAAQAIARALAGKAPFDLVFGLMANRS
                     VGEVLGPLAPLVRRAEVVPLPGHASHDPRDVAMFAQGELGLRACYPALGLEQALERLG
                     GDPDAAPVLVIAGSLYLAGDVLRRNGEVPD"
BASE COUNT          394 a         1003 c          765 g          413 t
ORIGIN      
        1 accctgcgcg cgcccgatac gccccggcct gaaaccaccg cgctccaccg tgaactcgcc
       61 gaacccggcg cgctcacccc ggcggctcgc ggcatcgcgc tcgccgtcgt cggcctgtcc
      121 tgggcctggg ccatcggcac catcgccatg ttcatgatct cgatgctggc cccggcccac
      181 ccggaagcgc cgctgccgtc ggtgggcgat ttcacgcgag cctatggccc atggatcatc
      241 ctcgccacgg tcggcgtgcc tctcgtcacc gccgccgtca ccaactggct gaaggcccac
      301 cgccgctcgg tcctgaccgc gcccgacacc gctcgctcga gcctgcggac cgccgccaac
      361 catctttacg tcgcgctggt ctcgcccttg gcggagcttt ccgcccggct cggctggggg
      421 gtgctggtcg tcatcggcat gatcctcacc tacaccctgt gctacaatat ctggtcgagc
      481 ttcgccttcc cgttctacct cgatttcctc aagtactcga aggacgaggt cgccttcgcc
      541 tccaaggtct tcggcatctt catgaccatg ttcggcatca gcctgggcgg aatcctgttc
      601 gcccggctgg ggcgcatgcc cacgatcctg atcggcgcgg tcctcccgat cctcggcaat
      661 ttcgtctatg ccgatctcgc cgaaggcgcc ccgctgatcg atgccttcgg ctcggccatc
      721 ggcctcaagg ccctgttcgg cctgttcggc ttcgacgaac ggatgatccg cctcttgctg
      781 gcgatctctt tcgaaaatgt ctccaccggc atcgccggcg cagccttcgt ggcctatctc
      841 tcggggatcg tcagcaagag ctacacggcc gtgcaatatg ccctgctctc ctcgctcacc
      901 ttcctgatcg gctcattggg ccgcggcatc gccggcgaag cctttgacca gttcggctat
      961 gccacggtct tccgctggac ggcggccgcg ggcctcgccg cggttttgtt cgtcctgctc
     1021 gaatgggcca gggtgcggcg tgcagagagg gcctgaaaaa tagtccggtc gtcctgagga
     1081 gcctcagcct tcggctttgg ctctcctcgg gacaaacgcg gtgccgggaa gctcagtccg
     1141 gcacttcccc gttccgccgc agcacgtccc cggcgagata gagcgatccc gcgatcacca
     1201 gcaccggcgc cgcgtcgggg tcacccccca gtcgctccag cgcctgctcc agcccaagcg
     1261 ccggatagca cgcccgcagc cccaattcgc cttgcgcaaa catcgccacg tcccgcggat
     1321 cgtggctggc atgccccgga agcggcacca cctcggcccg ccggaccagc ggcgccaatg
     1381 gccccagcac ttcgcccacc gagcgattgg ccatgagccc gaacaccagg tcaaacggcg
     1441 ccttgcccgc cagcgccctg gcaatcgcct gcgccgcatc ggcattgtgc ccgccgtcga
     1501 gccacacctc gcgccccggc accagcgccg tcagcggccc gtcgcctagc cgctgtaacc
     1561 gcgccggcca gcgcgcctcc cgcaggcccc gggcaatcgc ctcatcagaa attggcagcg
     1621 ccgactgcgc ccgcagcatg gccaccgcca gtgcggcatt gtctccctga tgggccccgg
     1681 gcagggcagg cagcggcaag gccaactcgc cgcgaccatc ccgaaaggca attccctcaa
     1741 tccccatgtc ggcccaccag tcccgccccc gcatcagcag gaccgcgcca tgggctaccg
     1801 ccgtgcgcac cacctcatcc gccgccagcg ccggataggc ctgcgtcacc agcggaaccc
     1861 ccggcttggc gatggacgcc ttctcgaacg cgatccgcgc cagcggctcg gccggcacac
     1921 cctcctcggg ccgcagcagg aaggcctcgt gatcgatccc cagcgcggcg atcccgcaga
     1981 ccagcggcgc gggaatcacg ttggttgcat cgaaccgccc gccaagcccc acctcgatca
     2041 cgcaggcatc ggccggcgtg cgcgaaaagg ccaggaacag cgcggcggtg gtcacctcga
     2101 agaagctcgg ccccaggtcc tctccggcat cgagcacctc ggccagcacc tcagccagcg
     2161 ccgcatccgc gatcagcctg ccggcaatgc ggatgcgttc gttatagcgc accagatggg
     2221 gcttggttgc ggtgtggacg gtatacccct gcgcctccag gaccgcgcgc aggaaggcgc
     2281 aggtcgaacc cttgccattc gtccccgcca cgtggaacac cggcggcagc cgccgctccg
     2341 ggttccccag ccgcgcgcac agctgcgtga tcgtctccag tccaaaccgc ccctgcggca
     2401 ggctcagcag cgccagccgg tcaagctgga gctggacgcg cggatcgtca gaagtagcaa
     2461 agtcacgcat cattcctccc cggaacgggg agggggacca ccgcaggtgg tggaggggca
     2521 ccggcggttc ttccatctcc tgggcgtaac gaataacggc gcgtggccct ccccc
//
LOCUS       DATNBV010000309         3668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2393982,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000309 DATNBV010000000
VERSION     DATNBV010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2393982"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..2321
                     /locus_tag="VN627_07435"
     CDS             126..2321
                     /locus_tag="VN627_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXD03125.1"
                     /translation="MQLKLNDSQTMLQDTVSRFFADEVTPARLREADDTGFDQALWDQ
                     LVALGIVGMRAVAPEDGGMTLFDAALVAEQAGRHVAPVPLIEGIVATGLLHRSGAPGS
                     LLAEVDGGAVATLALAPIKSGEAQLVSGAGPARVVVALDGDDLVALVGIAATPSANVG
                     GLAAAWVDLAAAGGERHVLASGDEARRLHQAAVEEWRVLNAIYLAGLGRRGLELAAAY
                     SGERMAYGVAIGTFQGIAHPMADAVTNMEGALLLGWRAIASIEAGDERAGARCAMAAW
                     WASHSVDQAVKHALRTFGGYGLSLEYDIQFYFRRAKFHALIAGNPDTLLDEVATRLWQ
                     DAAPALPAAGDSGIEFEWGEAAEAHAAEVRAFIEANLTDDVRAKIHHSTSGHHPGFHK
                     KMCEAGLAFPSLAVDGKPEGDRYATLAASSIWEEMGWNRTASAVTEFLAHMVDMCGTP
                     EVREEVLSGVLAGDALGCLGFSEPQSGSDLFGAKFSAVRDGDEWVLNGQKMFTTNGHN
                     AKYILLLTRTDNTGKKHQGLTMFVVPMDQPGVELHPVHTLQEEKTNITYFTDVRVPDR
                     YRLGEVNQGAKVMQLALGFEHGGIGYWFAHRAMMLRAEAWASRPLPDGTMPLDNIEIR
                     RALAAAAARSEVGDVLCRRSEWARIEGIHEIAWGPMAKVFSTESIYTDGSAIVAAAAP
                     ASLVRGLDKDMDIIEVTMRRGIGMTIYGGTSEVHRSLIAEKALGMPKSR"
     gene            complement(2628..2900)
                     /locus_tag="VN627_07440"
     CDS             complement(2628..2900)
                     /locus_tag="VN627_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03126.1"
                     /translation="MTFNSSAESPANTVLQSGLPFFVGRFQAHHIIPVATFGKTQTAD
                     SIGSFLLAMESTPKTRPPTGCGCLSMIRTPCCWAVPSTAGITVTGA"
     gene            complement(2897..3619)
                     /locus_tag="VN627_07445"
     CDS             complement(2897..3619)
                     /locus_tag="VN627_07445"
                     /inference="COORDINATES: protein motif:HMM:NF019408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1629 domain-containing protein"
                     /protein_id="HXD03127.1"
                     /translation="MRAWWAQQEWDSDEYRIVYQTEWVGDDGERYAEAKAQVEREIRE
                     KYLTPDPGPRPWYPPSPWDILHGVSPGIGFRFPVPDRPYRFQITERPTYLADIFPVRC
                     GEAISERVVEAIEQVEPGVHQYLPCELYQPDGSPIPERRWILNNCNRFDSVALGHCNV
                     VELSQTGKIAKGNGKTDIKVWRDVVAGHALWTDWRIMNSPLYVADALVERLRAMAAHG
                     WRFDHHLPEFSKTEIPNAGEQA"
BASE COUNT          652 a         1178 c         1173 g          665 t
ORIGIN      
        1 gtcccgccag gcccatccgt cttatgtaga aatttccctg cccctctgtg cgacttgcgc
       61 gcggcgttcg gcttgggcaa ggcgaccgcc acgaactagt gcaaccgaag gcggagtgcg
      121 ggcaaatgca gctgaagttg aacgatagcc agaccatgct gcaggacacc gtatcgcggt
      181 tcttcgcgga cgaagttacc ccggcccgtc tgcgggaggc cgatgacacg ggcttcgatc
      241 aggccctgtg ggatcagctg gttgccctgg gcatcgtcgg catgcgcgca gtggccccgg
      301 aagatggggg gatgaccctg ttcgatgccg ccctcgttgc cgaacaggcc gggcgccatg
      361 tcgcgccggt gccgctgatc gaaggcatcg tggccacggg cctgctgcat cgttccgggg
      421 cgcctggatc gttgctggcc gaagttgacg gcggcgccgt ggccacgctg gcgctcgccc
      481 cgatcaagtc cggcgaggcg cagctggtgt cgggcgcggg ccctgcccgg gttgtggtcg
      541 ccctcgacgg agacgacctt gtcgcccttg tggggattgc cgccacgccg tcggcaaacg
      601 ttggcggcct tgccgccgcg tgggttgatc ttgccgcagc cggcggcgag cgccacgtgc
      661 tcgctagcgg cgacgaagcc cgccgcctgc atcaggccgc ggtcgaggaa tggcgcgtgc
      721 tcaacgccat ttatctcgcc gggctcggcc gccgcgggct ggaactggcc gccgcctatt
      781 cgggcgagcg catggcctat ggcgtggcaa tcggtacctt ccagggcatc gcccatccga
      841 tggccgatgc cgtgaccaac atggaaggcg ccctgctcct cgggtggcgc gccatcgcct
      901 cgatcgaggc tggggacgag cgggccggcg ctcgctgcgc catggccgca tggtgggcga
      961 gccacagcgt ggaccaggcc gtcaagcacg ccctgcgcac gtttggcggt tatggcctct
     1021 cgcttgagta cgacatccag ttctacttcc gccgggccaa gttccacgcg ttgatcgccg
     1081 gcaatcccga cacattgctg gacgaagtcg ccacgcgctt gtggcaggac gccgcgccag
     1141 ccctgcctgc cgccggtgac agcggcatcg aattcgaatg gggcgaagcg gcagaagccc
     1201 atgcggccga agtgcgcgct ttcatcgagg ccaatcttac cgacgacgtc cgcgcaaaga
     1261 tccaccattc cacctcgggc catcaccccg gcttccacaa gaagatgtgc gaggctggcc
     1321 tggcttttcc gagcctggcg gttgacggaa agcccgaagg tgatcgctac gccaccttgg
     1381 ccgcatccag tatctgggaa gagatgggct ggaaccgcac ggcttccgcc gtcaccgaat
     1441 tcctcgccca catggtcgac atgtgcggca cgcccgaagt acgcgaggaa gtgctgtccg
     1501 gtgtgctggc gggggatgcc ctgggctgcc ttggcttttc cgagccgcaa tccgggtcgg
     1561 acctgttcgg cgccaagttc agcgccgttc gcgatggcga cgaatgggtg ctcaacggcc
     1621 agaagatgtt cacgaccaat ggccacaacg ccaagtacat cctgctgctg acccgcaccg
     1681 acaatacggg caagaagcac cagggcctga ccatgttcgt ggtgccgatg gaccagcccg
     1741 gcgtcgaact ccacccggtg catacgctcc aggaagagaa gaccaacatc acctatttca
     1801 ccgacgtccg cgtgcccgat cgctatcgcc tgggcgaagt gaaccagggc gccaaggtca
     1861 tgcagctggc gcttggcttc gaacacggcg gcatcggcta ctggttcgcc caccgggcga
     1921 tgatgctgcg tgccgaggcc tgggccagcc ggcccctgcc cgacggcacc atgcccctgg
     1981 acaacatcga aatccgccgc gcccttgccg ccgccgccgc ccgcagcgag gttggcgatg
     2041 tgctttgccg gcgctcggaa tgggcgcgga tcgaaggaat ccacgagatc gcctgggggc
     2101 cgatggccaa ggtcttctcc accgagtcca tctacaccga cggctcggcc atcgtcgctg
     2161 ccgcggcccc ggcctcgttg gtgcgcgggc tggacaagga catggacatc atcgaggtga
     2221 ccatgcgccg cggcatcggc atgacgatct acggcggcac cagcgaggtg caccgcagcc
     2281 tgatcgccga aaaggcattg ggaatgccca agtcgcggta agcggccgag gaaatcagcc
     2341 taccctccca ggcgccccgt cccgccgcgg gcgccccggc cggccgggcg caaggcgctt
     2401 gcctgtgcgc agtgccatgt ccgccagcca ctcggggctg cctcccagca tttgccgcgc
     2461 tgggcgaagt gatgggggcg tccgggcaag acgaagcggg tgaaccggga catgggggga
     2521 cttggcgggg cagacgggtg caatattcgc gtaaacgtgg acgtgttacc gtgattccgc
     2581 gaattccgcg ggcaagcgcg gacccaagcc gaagagtatt taggggttta cgcacctgtc
     2641 accgtaattc ccgctgtgct tggaaccgcc cagcagcaag gcgtcctgat catcgacagg
     2701 cagccacagc cggttggtgg ccgtgtcttc ggagttgatt ccattgccag caggaagctt
     2761 ccaatgctgt cggcagtttg cgttttaccg aaggtagcca ccggtatgat atggtgggct
     2821 tggaaccggc cgacgaagaa cggcagaccg ctctgcagca cggtgttggc tggactttcg
     2881 gctgatgaat tgaatgtcat gcctgttctc cggcattagg aatttcagtt tttgaaaact
     2941 cggggaggtg gtgatcgaaa cgccacccgt gcgcggccat tgcgcgcaat cgttcgacca
     3001 gggcatcggc gacataaagc gggctgttca ttatccgcca atcggtccat agggcgtgac
     3061 cagcaaccac gtcccgccaa accttgatgt cggtcttgcc gttgcctttc gcgatctttc
     3121 cggtttgcga caattctacg acgttgcaat gcccgagcgc gacgctgtca aaccgattgc
     3181 aattgttgag aatccagcgc cgctctggaa ttgggctacc gtcgggctgg tacagctcgc
     3241 acggtaggta ttgatgtacg cccggttcaa cctgttcgat ggcctcaacc acgcgttcag
     3301 aaatcgcctc gccacatcga accggaaaaa tgtcggcaag ataagtcggt ctttccgtaa
     3361 tctgaaaccg atacggccga tccggcactg gaaatcgaaa tccaattccc ggacttaccc
     3421 catgcaagat gtcccatggc gacggtggat accagggacg cggcccagga tcgggcgtca
     3481 ggtacttttc cctgatttcg cgctcgacct gcgctttggc ctcggcataa cgctcgccgt
     3541 catcgccgac ccattcggtc tggtagacga tccggtattc gtcgctgtcc cattcctgtt
     3601 gcgcccacca agccctcacg ctgcctccct gtaggcacaa acagacaagc ggaatgggaa
     3661 attacggt
//
LOCUS       DATNBV010000310         2426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4010304,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000310 DATNBV010000000
VERSION     DATNBV010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4010304"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VN627_07450"
     CDS             complement(<1..1053)
                     /locus_tag="VN627_07450"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HXD03128.1"
                     /translation="MSANPTVDLSAPPVITEAMPNPNASTSPADRIVFNVPAVTALGA
                     PEDQTLSAFRGPLPSQVTVTTLNGGVALEPAAPSFVASVRSQEPCDGLRLTPDCRPLG
                     TVPFLSLGVPQPRSGSLPSGQDERLAKELPDWPWQAIGRVNVADSVSRRFCTGTLVGP
                     RLVLTAGQCLFDYRLGRWVKPEHVHFVVGQVRDRFLGHSRAKELIVPPELRIAEGAPP
                     QRRAMRRAHIAYDWALIRLQDALPVKPLPVKKLSGLALMQEAAGHELARAGYGTHRPY
                     MLSVHRGCSARDVAAEPGLMLNECHPLAGDSGSPILLLKAGEAFVIGLSSGATFEWSA
                     DTGYVALAGLGASAAAF"
     gene            complement(1743..>2426)
                     /locus_tag="VN627_07455"
     CDS             complement(1743..>2426)
                     /locus_tag="VN627_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HXD03129.1"
                     /translation="RLAWAGGLALAICGVAPLGDALIQPLEARFQRPDLDRVGPSIAG
                     IIVLGGGEDGHAAGTPELAGLNEAAERYTEAVALARRLPAARIVFSGGSGRLLTVEAP
                     EAEEAGRLFAALGIAEDRITLESKSRNTHENAVLSAEIVKPAPGQRWLLVTSAWHMPR
                     AIGCFRRAGFTVDAWPVDYRAPRPLDITRVYGSVPEGLRRVDIVMREYVGLVAYYLTG
                     RIGSLLPGP"
BASE COUNT          415 a          848 c          785 g          378 t
ORIGIN      
        1 aaaggccgca gcagatgcac ccaggccagc gagcgcaaca tagcccgtgt cggcgctcca
       61 ctcgaatgtt gcgccactgc tcagaccgat gacgaacgcc tctcctgctt tcaacagcaa
      121 aattggcgaa cccgaatcgc cagcgagcgg atggcattcg ttcaacatca accccggctc
      181 cgccgccaca tctcgcgcag aacagccgcg gtggacggac aacatgtagg gccggtgcgt
      241 gccatatccc gcacgcgcca attcgtggcc ggccgcctct tgcatcaaag ccagccccga
      301 cagcttcttc accggcagcg gtttgaccgg gagcgcgtct tgcaggcgga taagcgccca
      361 gtcgtacgct atatgggctc tccgcatggc cctgcgttgg ggaggcgcgc cctcggcaat
      421 ccgcagctcg ggaggaacga tcagttcctt cgccctcgaa tggccgagga acctgtcgcg
      481 cacctgcccg acaacgaaat ggacgtgctc gggcttgacc cagcgcccga ggcggtagtc
      541 gaacaagcat tggcctgctg tgagcaccag ccgcggaccg acgagggtcc ccgtgcaaaa
      601 gcggcgagag acgctgtcgg ccacattcac ccgtccaatg gcctgccaag gccagtctgg
      661 caactccttc gccagacgct cgtcctggcc tgagggtagg ctcccagaac gcggttgggg
      721 cacgccgagt gagagaaatg gaaccgtgcc cagagggcgg caatcggggg tgagtctcaa
      781 accgtcgcag ggctcctgac tgcgaactga tgcgacgaag cttggtgcgg ctggctccaa
      841 tgcaacgccg ccgtttagcg ttgtcacagt gacctgtgac ggcagaggcc ctcggaacgc
      901 tgagagcgtc tgatcttcgg gtgcgccgag cgcggtgacg gccggaacgt tgaagacaat
      961 ccggtcggca ggagatgtcg aagcattggg gttcggcatc gcttcggtga tcacgggcgg
     1021 cgcactcaga tcaacggtcg ggttcgcgct cacgctcgtg accagacttg gagagctggc
     1081 tgggatcgaa ctcgcagccg ggccgcccac agacagcgct ggcgcgccac tcggaccctg
     1141 tatgtagctc ggtccagaat tcccagtccc gcttccaccc ggacctgggg catagctgcg
     1201 acccgatccc tggctcccgc tgtatggact cgatggactc gcgagcggca ttgggctcgg
     1261 agcgtagtag cgcggtgctg gacccggccc ctgatggccc ccggatcttc cggatgagtt
     1321 tcccgacgga cctgggtccg cgctggcccc tgcgccccaa gcaatgccca gggctgccac
     1381 tgcaatctgt gccgtacggc tgcgcaggtg tgtgagcatg ttcccccccg gcgcacgggt
     1441 cggccgtaga aagcgctcgc cccgacccgt cctcggtcgc tgatcagcga tttccgtctt
     1501 gccgatgtgc tgctgatccc actttgaacg acgcgattat agtttcacgg cctcggcttg
     1561 agaaaggtac gaacggccca cagacggtct aggccaaacg tcccaacatc catcgcgaac
     1621 cagctgtcgc actgcaaaat ctcgatcgtg tgcgacccag cctgccttcg ccgcgagtgt
     1681 gccgtgttcg gccgtgccaa ggtcgaccac cacggcgcgc gtgccgcggc ggcgctatgg
     1741 ccctacggcc ccggcagcag cgacccgatg cggcccgtca ggtagtaggc cacgagcccc
     1801 acgtactcac gcatgacgat atcgacccgc ctcaggcctt ccggcaccga gccgtacacc
     1861 cgtgtgatgt ccagcgggcg cggcgcgcgg tagtcgaccg gccaggcgtc gaccgtgaag
     1921 ccggcacgcc ggaagcagcc gatggcccgc ggcatgtgcc aggccgaggt gacgagcagc
     1981 cagcgctggc cgggcgcggg cttcacgatc tccgcgctga gcaccgcgtt ctcatgggtg
     2041 ttgcgcgact tgctctccag cgtgatcctg tcctcggcga ttcccagcgc cgcgaacagg
     2101 cgccctgcct cctcggcctc cggcgcctcc accgtcagca gccggcccga cccgccggag
     2161 aagacgatgc gggccgccgg caggcgccgc gccagcgcga cggcctcggt atacctctcg
     2221 gcagcctcgt tgagtcccgc cagctccggc gtccccgcgg cgtggccgtc ctcgccgccg
     2281 ccaagcacga tgatgccggc gatggacggc cccacgcggt cgaggtcggg gcgctggaag
     2341 cgggcctcca gaggctggat cagcgcatcc ccgagcggcg cgaccccgca gatggcgagc
     2401 gccagcccgc cggcccaagc gaggcg
//
LOCUS       DATNBV010000311         2821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2098134,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000311 DATNBV010000000
VERSION     DATNBV010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2098134"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VN627_07460"
     CDS             complement(<1..618)
                     /locus_tag="VN627_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXD03130.1"
                     /translation="MVRFENVGLRYGLGPEILRDLTFLIPAHSFQFLTGPSGAGKTSL
                     LRLLFLSIRPTRGLVNLFGQDVSLLGKDQVANMRQKIGIVLQDFRLLDHMTTYENVAL
                     PFRVMGREESSYRREVIDLLKWVGLGERMDALPPILSGGEKQRAAIARAVISRPQLLL
                     ADEPTGSVDPTLGRRLLRLFIELNRLGTAVIIATHDITLMDQYEAR"
     gene            976..1719
                     /locus_tag="VN627_07465"
     CDS             976..1719
                     /locus_tag="VN627_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03131.1"
                     /translation="MAAAMPATMPSQAQSAPPAENDAAAEWDALAHEEEGQDAPVVDS
                     PSISAGWPAEGEASQPGGDSDWPSAARQEAEDHEEIPITTHRQRLARLFRLPALPRLP
                     AAGLPTACAAMGALILALMIWRADVVRLLPQTATFYKMVGLEVNLRGLAFKDIKVSNE
                     TVDGKPVLVIEGTIIGETKKPVELPRLRFSVRDAQGTEIYAWNAVLEQPVLKPGERAY
                     FKSRLASPPPEGRNIDVRFFNKRDLAGHA"
     gene            1761..2138
                     /locus_tag="VN627_07470"
     CDS             1761..2138
                     /locus_tag="VN627_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXD03132.1"
                     /translation="MPRVLIADDEDSMRLLVARAIAMDGHETVTAEDGAEALEILTRE
                     QGRFDLLLTDIQMPVMDGIALALAAARDFPDLKILLMTGFAAQRERASGLSAIVHDVV
                     TKPFAVANIRTAVADALAARKNG"
     gene            complement(2295..2669)
                     /locus_tag="VN627_07475"
     CDS             complement(2295..2669)
                     /locus_tag="VN627_07475"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HXD03133.1"
                     /translation="MGDASGRLGEGNADGAVDSQGRALEALRKGAQSLAEAMQQGDGD
                     QPGDGPGNPRGRQQGAANSTDPLGRPMRHNEFSDDYTVKIPGEIDVQRVRRILEELRR
                     RLADPSRPQIELDYIERLLKDY"
     gene            2610..>2821
                     /locus_tag="VN627_07480"
     CDS             2610..>2821
                     /locus_tag="VN627_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03134.1"
                     /translation="MRVHRTVGIALAEPAAGIAHRGIGLIEPVLAVAGIAALTWLALA
                     LLHALLAALALLPLLTALTRPHAALG"
BASE COUNT          484 a          943 c          918 g          476 t
ORIGIN      
        1 ccgcgcctcg tactggtcca tcaacgtgat gtcgtgggtc gcgatgatga cagcggtgcc
       61 cagcctgttc aattcgatga acagccgcag caggcgccgg ccgagcgtcg gatcgacgct
      121 gccggtcggt tcgtccgcca atagcaattg tggccgcgag atcaccgcgc gcgcgatcgc
      181 ggcgcgctgc ttctcgccgc ccgacaggat cggcggcagc gcatccatgc gctcgccgag
      241 gccgacccat ttcaggaggt cgatcacctc gcggcgatag ctggattctt cgcggcccat
      301 cacgcggaac ggcagcgcca cgttctcata ggtcgtcatg tggtcgagca ggcgaaaatc
      361 ctgcagcacg atgccgatct tctggcgcat gttcgcgacc tgatccttgc cgagcagcga
      421 gacgtcctgg ccgaacaggt tgacgagacc gcgcgtcggc cgaatcgaca gaaacagcag
      481 ccgcagcagc gaggtcttgc ccgcgcccga ggggccggtg agaaactgga aggaatgcgc
      541 cggaatcagg aaagtaaggt cgcgcagaat ctccgggccg agcccgtacc gcaaaccgac
      601 attttcgaac cgaaccaagc tcagctccgc tcgacatgaa ccgtggccga gctccgctcg
      661 gtttgggccg cggccataaa ccccggactt gaacctgaac ctggacccga acccggactt
      721 tgggcgcgtg gcccgctggt ttgggaacca accggacaaa acggttttgc gaccgttatg
      781 gtttccgatt cgttaacggt cgctatgtag catcccggca aagacacggt agagatgggc
      841 tccatgcata ttatttgccc tcattgtaca acatcttacg cgatcaatcc ggccaccctg
      901 ggatccgccg gacgcaccgt ccgctgctcg cgctgcaagg agacctggct ggcgcggccc
      961 gaggatgcga tcgaaatggc ggccgccatg ccggccacca tgccaagtca ggcgcaatcg
     1021 gccccgcctg ccgaaaacga tgccgcggcc gaatgggacg cgctggcgca cgaggaagag
     1081 gggcaggacg cccctgttgt cgacagcccc tcgatttcgg ccggctggcc ggccgaaggc
     1141 gaagcttcgc agcctggcgg cgacagcgac tggccgtcgg ctgcccggca ggaggcggag
     1201 gaccatgaag aaatcccgat caccacgcac cgccagcggc tggcccggct gttccggctg
     1261 cccgccctgc cgcgcctgcc ggcggcgggc ctgccgaccg cctgcgccgc aatgggcgcg
     1321 ctgatcctgg cgctgatgat ctggcgcgcc gacgtcgtgc ggctgctgcc gcaaaccgcg
     1381 acgttctaca agatggtcgg gctggaagtg aacctgcgcg ggctggcatt caaggacatc
     1441 aaggtctcca acgagaccgt ggacggcaag ccggtgctgg tcatcgaagg cacgatcatc
     1501 ggcgagacca agaaaccggt cgaattgccg cggctgcgct tttccgtccg cgacgcgcaa
     1561 ggcaccgaaa tttacgcctg gaatgcggtg ctcgagcagc cggtgctcaa acccggcgag
     1621 cgtgcctatt tcaagtcgcg gctggcttcg ccgccgcccg agggccgcaa tattgacgta
     1681 cgctttttca acaagcggga tttggcaggc cacgcctgaa ggaaggccgc cttccccgct
     1741 ggggcgagag agacgatttc atgccacgcg tgctgattgc cgatgacgag gattcgatgc
     1801 gcctgctggt ggcacgcgcc atcgccatgg acggccacga gaccgtcacc gccgaggacg
     1861 gcgccgaggc gctcgaaatc ctgacccgcg aacagggtcg cttcgacctg ctgctcaccg
     1921 acatccagat gcccgtgatg gacggcatcg cgctggcgct ggcggcggcg cgcgattttc
     1981 cggacctgaa gatcctgttg atgaccggct tcgccgcgca gcgcgaacgc gcctcgggcc
     2041 tcagcgccat cgtccacgac gtggtgacga agccgttcgc ggtggccaat atccgcactg
     2101 cggtggcgga tgcgctggcg gcaaggaaga acgggtagct cagatgatga gcgtggatgg
     2161 caccccgtcc tccgctcgtc attgccgggc ttgacccggc aatccatcgt ttcagaatgc
     2221 tcttgcgaag gtgatggata cgcgggtcaa gcccgcgtat gacaatcgtg gttccggcag
     2281 cagcagcgcg agccttaata atccttcagc agccgctcga tgtaatcgag ttcgatctgc
     2341 gggcgggacg gatcggcgag acggcgccgc agttcttcga ggatccggcg cacccgctgc
     2401 acatcgatct cgccggggat cttcacagta taatcgtcgc tgaattcgtt gtggcgcatc
     2461 ggccgtccca gcggatcggt ggaattggcc gcgccctgtt ggcggcctcg gggattgccg
     2521 gggccgtcgc ccggctggtc gccgtcgccc tgctgcatgg cctcggccag gctctgggcg
     2581 cccttgcgca acgcttccag cgcgcggcct tgcgagtcca ccgcaccgtc ggcattgccc
     2641 tcgccgagcc ggccgctggc atcgcccatc gcggaatcgg cctgatcgag cccgtcctcg
     2701 ccgtcgccgg gatcgccgcc ctgacctggc tggccctggc cctgctgcat gccctgctcg
     2761 ccgcgctggc cttgctgccc ttgttgaccg cgctgacccg gccccatgcc gcgcttggcg
     2821 a
//
LOCUS       DATNBV010000312         3590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_195132,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000312 DATNBV010000000
VERSION     DATNBV010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195132"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VN627_07485"
     CDS             complement(<1..239)
                     /locus_tag="VN627_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03135.1"
                     /translation="MSTDKTGSGPNVPDDQQLSAWLDGGLDDDGNAKMADLAARNEDV
                     ASRAARLRHMDELVRAAVPAEEEIPAELLTRLGLAP"
     gene            complement(236..772)
                     /locus_tag="VN627_07490"
     CDS             complement(236..772)
                     /locus_tag="VN627_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXD03136.1"
                     /translation="MTSAAAPAARAPFQAQLVALLPRLRRFCHGLTGNRDLGDDLLQI
                     AVERALARFEQWEPGSSLENWVMKIASNANIDRLRAEKVRGTAIEIDEALDLPGEDQL
                     AMLEFRSELEAARAALAAMPSHLREVMATVVIDGRSYREAAELLEVPIGTVMSRVSRA
                     RQFVEAYVRRGPERMVAA"
     gene            complement(980..>3590)
                     /locus_tag="VN627_07495"
     CDS             complement(980..>3590)
                     /locus_tag="VN627_07495"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS domain-containing protein"
                     /protein_id="HXD03137.1"
                     /translation="NAAPALTGTPAVLADGAEDTAYTVTAADLLAGFTDANGDALSLG
                     TVSADHATVANNGDGTYTVTPAANYNGALVLSYTVADSNGASTAVTLGLTLAAVNDAA
                     SAGGTIAGAVPESGGTAAGVVTVSDVDAGEAVFRPADAAALAGTYGAFTFDAASGAWT
                     YAVDPARAVTQALAPGELATETLTVTSLDGTASETITVTITGTSSIATIAGSATGAVD
                     AASGAAGGTLVVSDGDPGEAHFAALVAAALSGTYGAFAFDTATGAWTYALDSAGAAFT
                     ALGGGATANESLTVTSADGSASQTIVVTVTGANDAATITGSASGAVSEAGGLANALPG
                     TATASGTLGVADPDSGEAVFAAPASLAGTYGTFAFDAATGAWSYTLDNARAATQALGA
                     GAAASDVLTVHSLDGTAAQAITVAITGADDAAVITGRASGATTEDSTTPVSGLLHIAD
                     PDAGQAVFAAPASLAGTYGSFTFDAATGAWTYALDNARAATQALAGGQVVTETLNVAS
                     LHGDATQAITVAVTGRNDAAVIGGATSGAVTEAATTGGVSQVSGALTISDVDSPASFA
                     GGTYAGTYGSLSLTAAGAWTYSLDNANPTVDGLSAGQLLGDSVTVHAADGTTQAIAIQ
                     ITGVDEFVGGGVQTGTAGADTLTGSVGNDQLSGLAGNDRLLGLDGNDTLDGGAGDDVM
                     DGGTGTDTASYASATGSVTVNLALTVQQNTGGWGKDTLTGIENLVGSASADTLTGSAG
                     ANAIDGGAGDDRITGGLGADRLTGGAGRDLFVYTAVSDSSGAGHDTIVDFVRLTDRID
                     LSAIDPILTTNKNDAFLWGGTAAQTFGVWFTYDAATNTTHVFGDTDGNSATAELWIDL
                     AGNVALTQSDFVL"
BASE COUNT          490 a         1275 c         1267 g          558 t
ORIGIN      
        1 ggggccaagc ccaaccgggt cagcaactca gcgggaattt cctcttcggc gggcaccgcg
       61 gcgcgcacca gttcgtccat gtggcgcagc cgcgcggcgc ggctggcgac atcttcattg
      121 cgggcggcaa gatcggccat cttcgcattc ccatcgtcat cgagcccgcc atcgagccag
      181 gcggacagtt gctggtcgtc cgggacattc ggtccggagc cggtcttgtc ggtgctcatg
      241 ccgctaccat cctttccggt ccgcgccgca cataggcctc gacgaactgc cgcgcccgcg
      301 acacccggct catcaccgtg ccgatcggca cctcgagcag ctccgccgcc tcgcgatagg
      361 agcgcccgtc gatcaccacc gtggccatga cctcgcgcag gtgcgaaggc atcgcggcaa
      421 gggcggcgcg cgcggcctcc agctccgagc ggaactcgag catggcgagc tggtcctcgc
      481 cgggcaggtc cagggcttcg tcgatctcga ttgccgtccc gcgaaccttc tcggcccgca
      541 ggcggtcgat gttggcgttg cttgcgatct tcatcaccca gttctccagg ctcgagccag
      601 gctcccattg ttcgaagcgg gcgagggcac gttccaccgc gatctgcagc aggtcgtcac
      661 ccaggtcccg attgcccgtc agcccatggc aaaaccgccg cagccgcggc agcagggcca
      721 cgagctgcgc ctggaatggc gcgcgggcag caggggcagc ggcactcgtc atcgaacctc
      781 caccagcgtc aacggggctc gatgggaatt attccacgcc ggtttaccat gtcccgtttc
      841 gggaatggtt tgccccctgc gggcgatcat ggccgcggcg cgccgtcggg gcaactgttg
      901 ctgcaatcga taggggtacc gcagcccggc cagctagggg cagctggggg tggccgggct
      961 gcggatctcg tcagtcaggt cagagcacga agtccgactg cgtcagggcc acgttgccgg
     1021 caaggtcgat ccacagctcg gccgtggccg aattgccgtc ggtatcgccg aagacatggg
     1081 tggtgttggt ggccgcgtcg taggtgaacc acaccccgaa ggtctgggcc gcggtgccgc
     1141 cccacaggaa ggcgtcgttc ttgttggtgg tcaggatcgg gtcgatcgcg gagaggtcga
     1201 tccggtcggt gaggcggacg aaatcgacga tcgtgtcgtg gccggcgccc gaggagtcgc
     1261 tcaccgccgt gtagacgaac aggtcgcgac ccgcaccgcc ggtgagccgg tccgcgccga
     1321 gcccgccggt gatccggtca tcgcccgcgc cgccgtcgat ggcgttggcc ccggcactgc
     1381 cggtgagcgt atcggccgag gccgagccca cgagattttc gatccccgtc agcgtgtcct
     1441 tgccccagcc gccggtgttc tgctggacgg tgagcgcgag gttgaccgtg accgagccgg
     1501 tggcgctggc gtaggaggcg gtgtcggtcc cggtgccgcc gtccatcacg tcgtcaccgg
     1561 ccccgccgtc gagagtgtcg ttgccgtcga gcccgagcag ccggtcgttc ccggcaagac
     1621 cagacagctg gtcgttgccc acgctgccgg tgagcgtgtc cgcccccgcc gtgccggtct
     1681 gcaccccgcc gccgacgaat tcgtcgaccc cggtgatctg gatcgcgatg gcctgggtgg
     1741 tcccgtcggc cgcgtggacg gttacgctgt cgcccagcag ctggccggcg ctgagtccgt
     1801 cgaccgtggg gttggcattg tcgagcgaat aggtccacgc gcccgccgcg gtgaggctga
     1861 gcgagccgta ggtcccggca taggtccccc cggcaaagct cgccgggctg tccacatcgc
     1921 tgatcgtcag cgcgcccgac acctgcgaga ccccgccggt cgtcgccgcc tcggtcacgg
     1981 cgccggaagt cgccccgccg atcaccgccg catcgttgcg gcccgtcacc gcaaccgtga
     2041 tcgcctgggt ggcatcgccg tgaagcgagg ccacgttaag cgtctcggtg acgacctgcc
     2101 cgccggccag agcctgggtc gcggcgcggg cattgtcgag cgcataggtc caggctccgg
     2161 tggcggcgtc aaaggtgaag ctgccatagg tcccggcgag gctcgccgga gcggcaaaca
     2221 ccgcctggcc cgcgtcggga tcggcaatgt ggagcaggcc ggaaaccggc gtggtgctgt
     2281 cctcggtcgt cgccccgctg gccctgccgg tgatcactgc cgcatcatcg gccccggtga
     2341 ttgccacggt gatcgcttgg gccgccgtgc cgtcaagcga gtgcacggtc agcacgtcgc
     2401 tggccgcagc ccccgcgccc agcgcctggg tggcggcgcg ggcattgtcg agcgtgtagg
     2461 accaggcgcc ggtcgccgcg tcgaaagcga aagtgccgta ggtcccggca aggctggccg
     2521 gggcagcgaa cactgcctcg ccgctgtccg gatcggccac gccgagcgtg cccgaagccg
     2581 tggcggtacc gggcagggcg ttggcaaggc cgcccgcctc actcaccgcg ccgctcgcgc
     2641 tgccggtgat ggtggcggca tcgttggcgc cggtgacggt caccacgata gtctggctcg
     2701 cgctgccgtc ggccgaggtg acggtcaggc tctcgttcgc cgtcgccccg ccgccaagcg
     2761 cggtgaaggc tgccccggcg ctatcgagcg cataggtcca ggcacccgtg gccgtgtcga
     2821 acgcgaaggc gccgtaggtg cccgaaagcg cagccgcaac gagcgcggcg aagtgcgcct
     2881 ccccgggatc gccatcgctc accaccagcg tgccgcccgc cgcgccgctg gcggcatcga
     2941 ccgcaccggt ggcgctgccc gcaatcgtgg cgatcgagct ggtgccggtg atggtcaccg
     3001 tgatcgtctc gctggccgtg ccatcaagcg aggtcacggt cagcgtttcc gtcgccagct
     3061 cgcccggagc cagcgcctgg gtcaccgcgc gcgccggatc gacggcatag gtccaggccc
     3121 cgctcgccgc atcgaaggtg aaggccccgt aggtcccggc cagcgccgcc gcgtcggcgg
     3181 gccggaacac cgcctctccg gcatcgacat cgctcacggt aaccacgccc gccgccgtgc
     3241 cgccgctttc cggcacggcc ccggcgatgg tgccacccgc gctggctgcg tcgttgaccg
     3301 cggcaagggt caggcccagc gtcaccgccg tgctggcccc gttgctgtcc gccacggtgt
     3361 aggacaagac cagcgcgccg ttgtaattgg ccgccggggt cacggtgtag gtgccgtcgc
     3421 cgttgttggc gaccgtggcg tgatcggcgc tcaccgtgcc gagcgacagg gcatcgccgt
     3481 tggcatcggt gaagccggcc agcaggtccg ccgcggtcac ggtataggcc gtgtcctcgg
     3541 cgccgtccgc cagcacggcg ggcgtgccgg tcagcgccgg ggcggcattg
//
LOCUS       DATNBV010000313         3399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1464048,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000313 DATNBV010000000
VERSION     DATNBV010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464048"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1137
                     /locus_tag="VN627_07500"
     CDS             127..1137
                     /locus_tag="VN627_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HXD03138.1"
                     /translation="MSISVLSSGTTGKPKLVEVSSLIFSRALATMAVSDVPGVPIRPN
                     LAFTPISNISGLIQFLGSIARGGCVFLLERFEMERWLTALEACAPDTLIMFAPMIHAF
                     LEKNVPKERFGGLRAVFGGAGPLAPEVQKAFQERYGIPIYWGYGCTEIAGTAASWSPA
                     LAEEFGASKLGSAGRAIPGVELRVVDPDSGDEVPRGETGLLEARVELLGPDWIRTTDL
                     AAIDSDDFLFLRGRADQAINRGGFKILPETIAAAAEEHPDVREAVAFGIPDARLGQVP
                     VVAVQPLEGTSLSEEQIITYLRTKLAAYAVPTKVLILDEIPRTGALKPDLPGLKSAAM
                     QD"
     gene            1312..2076
                     /locus_tag="VN627_07505"
     CDS             1312..2076
                     /locus_tag="VN627_07505"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXD03139.1"
                     /translation="MGMLQDKVIVVTGAGNGIGEASARCFAAHGARLLISDIAGDQAE
                     RVAAEIRAGGAQAQAMTVDVTDEAQVIAMVYAAVSRYGRLDGALNNAGGGCAPAPTAE
                     VTLADMHRVMDLNFTSSWLCTKYEIQAMLKTGGGSIVATASDAGLLGTPNMAPYAAAK
                     AALINLIRTAAVEYGKQGIRLNAVCPGATATPALQRMAEQVGGDISVILGDRPIPRLG
                     EPREVAEAAAWLLSDLSSFVTGQPLAVDGGFNAAFT"
     gene            complement(2110..3159)
                     /locus_tag="VN627_07510"
     CDS             complement(2110..3159)
                     /locus_tag="VN627_07510"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXD03140.1"
                     /translation="MSRGICEEDFGPMIKLSMKPTGWFQIGWSGEIPPGGVKPLRYFG
                     MDLVAYRSASGVLNVMEAHCKHLGAHLAYGGKVKGDCIVCPYHGWEWNSEGANTLVPY
                     QDQPTGAKLRKFHTIERHGIMFMWHDPAGGGPRGEGWDLPDLFRDIPQAVADEADFYP
                     CWPDAVVNKPGEPIHPQMVVENSPDTSHFHFTHGTPIPPDLEWFDTSSGAWRSEMAFY
                     SPKTKEKALSLFVLCPCISLSYTIFDGGSVKYRLILTATPVDDQTSDFRVNYFFPRDP
                     TSPDVMPESLREFARSTVELFEEDARMWRHQVFVQKPVYSKKDIAGYTAMRKWSERFY
                     ETEPGPGPMRSVDRL"
BASE COUNT          586 a         1151 c         1087 g          575 t
ORIGIN      
        1 ctggcagtcg agagcgcccg cgaagccggc acgatcggca ttcgctgcga gggtcgcaac
       61 agcctcaagc tatcggttga gccgaggctg gaagtgctcg gtcaagacca ccgcccggcg
      121 cagccgatgt cgatttccgt cctctcgagc gggaccacgg ggaagccgaa gctggtcgag
      181 gtgagctcac taatcttcag cagagcactc gccacgatgg cggtttccga tgtgcctggt
      241 gtgccgatca ggcccaacct ggccttcacg ccgatcagca acatcagcgg cctgatccag
      301 tttctgggca gtatcgcgcg cggcgggtgc gttttcctgc tcgaacgttt cgagatggag
      361 cgctggctga cagcgctcga ggcctgcgcg ccggacaccc tcatcatgtt cgcgccgatg
      421 attcatgcgt tcctcgagaa aaatgtcccg aaagagcgct tcggtggcct gcgagcggtg
      481 ttcgggggag cgggcccgct tgccccggag gtgcagaagg ctttccagga acgctacggc
      541 atcccgatct actggggcta cggctgcacc gaaatcgccg gaacggcggc aagctggagc
      601 ccggccctgg cggaagaatt cggcgccagc aagctcggga gcgcaggccg cgccatcccg
      661 ggcgtcgaac ttcgcgttgt cgatccggac agcggcgacg aggtgccccg gggggagacc
      721 ggcctgcttg aggcgcgcgt agaactgctc ggcccggact ggatccgcac gaccgatctt
      781 gccgcgatcg attccgacga tttcctgttc cttcgtggcc gcgccgacca ggcgatcaac
      841 cgcggtggct tcaagatctt gccggaaacg atcgccgctg cagccgaaga gcatcccgac
      901 gtgcgcgagg cggtcgcttt cggcataccc gatgcccggc tgggccaggt gccggtggtg
      961 gccgtacagc ccttggaagg taccagcctg tccgaggaac agataatcac ttatcttcgc
     1021 accaagcttg ctgcctacgc agttccgacc aaggtgctga tcctcgatga aattccccgc
     1081 acaggcgcgc tgaaacccga cctgcccggg ctgaagagcg cggccatgca ggactaatcg
     1141 cgcggcgatg caccgtcgtt gagaactgac cgcccggctg gagcaggccg ggcacagcgc
     1201 tcgcggtggc aggcgcggtt gccgaaggcg agcggcgcaa ttacgtctgt tttttcagcc
     1261 gtgatttcgt cagagtgccc gggcgaacgc accagacgaa agaccgcgac gatgggcatg
     1321 ctgcaggaca aggtgatcgt ggtgaccggc gccggcaacg gcatcggcga ggcttcggcc
     1381 aggtgctttg ccgcgcacgg cgcccgcctg ctgatctccg atattgccgg cgaccaggcc
     1441 gagcgcgtgg cggccgagat ccgcgccggc ggcgcccagg cccaggcgat gacggtcgac
     1501 gtgaccgacg aggcccaggt catcgccatg gtctatgccg ccgtcagccg ctatggccgc
     1561 ctggacggcg cgctcaacaa tgccggtggc ggctgcgccc ccgcgccgac ggccgaagtc
     1621 accctcgccg acatgcaccg ggtgatggac ctcaacttca cctcgtcgtg gctgtgcacc
     1681 aagtacgaga tccaggccat gctgaaaacc ggcggcggct cgatcgtcgc cacggcctcg
     1741 gatgccggcc tgctcggcac ccccaacatg gcgccctatg ccgccgccaa ggccgccctc
     1801 atcaacctga tccgcaccgc cgccgtcgaa tacggcaagc agggcatccg cctgaatgcc
     1861 gtctgccccg gggcgaccgc gacgccggcg ctccagcgca tggccgagca ggtcggcggc
     1921 gatatctccg tgatcctcgg cgaccgcccg attccgcgcc tgggcgaacc gcgcgaagtg
     1981 gccgaagccg cggcctggct gctgtccgac ctgtcctcgt tcgtgaccgg tcagcccctc
     2041 gccgtcgacg gcgggttcaa cgccgccttc acctgagaga ccctgacctg ctctgaccgc
     2101 cgcgtcccgt cagagcctat cgaccgagcg catcggcccg ggcccgggct cggtctcgta
     2161 gaaccgctcg ctccacttgc gcatggcggt gtagccggca atgtccttct tcgaatagac
     2221 cggcttctgg acgaagacct ggtggcgcca catccgcgcg tcctcctcga acagttcgac
     2281 cgtgctgcgg gcaaattcgc gcaaggactc gggcatgaca tcgggcgaag tcggatcgcg
     2341 ggggaagaag tagttcaccc ggaagtccga agtctggtcg tcgaccggcg tagccgtgag
     2401 gatcaggcgg tatttcaccg agccgccgtc aaaaatcgtg tacgacaggc tgatgcaggg
     2461 gcacagcacg aaaagactga gcgccttctc cttggtcttg ggcgaataga acgccatttc
     2521 cgagcgccag gccccgctgg aagtatcgaa ccattcgagg tccgggggga tcggcgtccc
     2581 gtgggtgaag tggaaatggc tggtgtcggg tgaattctcg accaccatct gcgggtggat
     2641 cggctcaccc ggcttgttga cgaccgcatc tggccagcaa gggtagaaat ccgcttcgtc
     2701 ggccacggct tggggtatgt cccggaacag gtcgggcaga tcccacccct cgccgcgcgg
     2761 gccgccgccc gccggatcgt gccacatgaa catgatcccg tggcgttcga tggtgtggaa
     2821 cttgcgcagc ttcgcgccgg tgggctggtc ctggtagggg acgagggtat tggcgccctc
     2881 gctgttccat tcccagccgt ggtacggaca tacgatgcaa tcgcccttga ccttgccccc
     2941 ataggcaaga tgcgcgccaa ggtgcttgca atgagcctcc atcacgttga gcacgccgga
     3001 tgccgagcga taggcgacga ggtccatccc gaagtagcgc agcggcttga cgccgcccgg
     3061 cgggatttcc ccgctccagc cgatctggaa ccaaccggtg ggcttcatcg acagcttgat
     3121 catcgggccg aaatcctctt cgcaaatgcc ccggctcacc tatctccctt gcccccggcc
     3181 cgcaagatat ttcgagtttc gaaatatctc tggcgcgtcc catcccctgg tctatgctcc
     3241 acgcaatcca acggtgtaac cgcatgaacg accccgcctc cgaagcatca gcactgaccg
     3301 cgcgctccgc cgcgccgaag gcggccgtac ccctcccgtc cggccccatc ggccggttgc
     3361 tggcctccgt cggcaacctc tcctacctgc tcaacgcca
//
LOCUS       DATNBV010000314         5497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_483630,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000314 DATNBV010000000
VERSION     DATNBV010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483630"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VN627_07515"
     CDS             complement(<1..623)
                     /locus_tag="VN627_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HXD03141.1"
                     /translation="MATTNLPDRYLAPSLPALLDGTRLPSAGLPHLEGDGVDLRYLLA
                     LFRRRLSLFVMVIALCVMLALNVTMLMPRLYRANADVVMNRDRTELVPEGRPADPSLT
                     APPRAEEIDTETKVIGSRDLAGRVADALRLEQDQAFLAQITRGGLIGRVKAALGMAPA
                     QPADRRRTVIDALLARVDASRLDNAYAIRISYTDPDPARAAQIANAFA"
     gene            complement(646..1998)
                     /locus_tag="VN627_07520"
     CDS             complement(646..1998)
                     /locus_tag="VN627_07520"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXD03142.1"
                     /translation="MNPVTLPSGPIRRPYPQRAARLCGLVLALGAGVGSGRALAQSAP
                     PRDPFEAGPQGALLRDDVAGARKIPGYTAPGLDLGGMHLAPSLTVRSEASSNVFNRSG
                     GGRGDVWATVAPAVLAVGQTGTARFVVDGRASLARYARLSGYNTDTWALRGQAARPVG
                     GGLTLALSGAAARRLEPPFEAAGANPFDGGVVLVDQLQAGLGVRVDLGRTRLTGAVDL
                     ARTHYLPVLGPGGAMISQAFRDERATGLSLRVDRSLGAGRIAFAEASLRQVRSLHPGT
                     APDRTGRVGEALVGLKGEFSHLVMGELAAGWQWRNYRSPALRNYRGLAWRARLEWYAT
                     PLVTFTLAGRRDLVNSPLPGAAGVVVDRVELKALYEVKRNLNLVLSAAHVVERYRDLP
                     GAWPTIRTNAAGIEATYAANRHVQLGLTARYRDRQSASFLLPRQGHAVEGGLFLRFSL
                     "
     gene            complement(2336..3091)
                     /locus_tag="VN627_07525"
     CDS             complement(2336..3091)
                     /locus_tag="VN627_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03143.1"
                     /translation="MTGATGRLAGRRILITGAASGIGAATARLFAGEGAALALLDVQA
                     GLLAEIATDIGASAIPVDLCDPAATTAAVNAAAMALGGLDGVINCAGVTASHPLGEWT
                     LEAWAKVIDTNLTAPFLVCQAALPHLRQAGTATIVTIASGTGLLPTGPGVSTYAASKA
                     GVIALMKAYAGELAPAIRVNSIAPGIVDTPMVSHVLGGYDNPDDAPFVRQYALKRVAR
                     PEEIARACLFLTSEESSFVTGTVLAVDGGRTFH"
     gene            complement(3088..4425)
                     /locus_tag="VN627_07530"
     CDS             complement(3088..4425)
                     /locus_tag="VN627_07530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD03144.1"
                     /translation="MTADDASVTPPSRIDSWPVAPRLAWFSVVVLMLATILGYLDRQI
                     FSLLVGPIRSSLEITDFQFSLLQGVAFGLFYAVFGLPFGWLVDRTSRRAVITVGVLLW
                     SAATIGCGLSQNVQQLALARFAVGVGEAALLPAVFSLLADIFPQRRLGLAFGLFGTGS
                     TIGSALAYSGGGLLIRHFEKLGGATLPLVGALQPWQVVFVIVGLPGAVVAFLPWLVPD
                     LRKGRKIERKASPKGLLLAFIGKNPTYFLCHFGGFGLNAVLAFGTAAWTPALLTRHMG
                     LDIAQAGLLMGIIGSTIGLLGYAGNGWLVDRWFAAGQRDAHLRYYAYSFVFMAVVAPI
                     AFLSTSLWVFVPLYALVFVLLPFSGPAVAHLQMATPPELRGQVSALFALSYNLMGMTL
                     GPSGVALVTDYVFHDPMMIHKSMAIMFFTTAVLGAIVFRLGLKPSRRALGDAV"
     gene            complement(4422..>5497)
                     /locus_tag="VN627_07535"
     CDS             complement(4422..>5497)
                     /locus_tag="VN627_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXD03145.1"
                     /translation="VEGSAMMYSYGTTGAPKGIIRPLGGHPAGTPGPGEDLITRGYGF
                     NAETVFLCPGPLYHAAPISWTTATQVMGGTSVVFRSFDPEAVLAAIEQHRVTHALFVP
                     THFVRMLKLPEEVRARYDLSSLKAVIHAAAPCPPEVKRAMIDWLGPVIEEFYGASEGG
                     FVKVSSAEWLERPGTVGRSIAGPIRILGDGGEDLPQGETGTIYFRLSSPMVYHNEPDK
                     TRATIIDDIWCTAGDVGYLDAEGYLFLADRKHNMIISGGVNIYPQETENVLTLHPAIA
                     DLAVIGVPNAEYGEEVKAVVALAEGVAPSDELAAELIAYCRERIAHYKCPRSVDFIAE
                     IPRLPSGKVRKHELLARYQETTR"
BASE COUNT          964 a         1776 c         2008 g          749 t
ORIGIN      
        1 gcgaaggcat tggcgatttg tgccgcgcgg gcgggatcgg gatccgtgta ggaaatgcgg
       61 atggcatagg cattgtcgag ccgggaggca tcgacccggg cgagcagggc atcgatcacg
      121 gtgcggcgcc ggtctgccgg ctgggcgggg gccatgccaa gggcggcctt cacccggccg
      181 atcagaccgc cgcgggtgat ctgggcgagg aaggcctggt cctgctccag ccgcagcgca
      241 tcggccacgc gcccggcaag atcgcgcgag ccgatcacct tggtttcggt atcgatctcc
      301 tcggctcgcg ggggcgcggt gagcgaggga tcggcggggc ggccttcggg caccagttcg
      361 gtccgatccc ggttcatcac cacgtcggcg ttggcgcggt agagccgggg catcagcatg
      421 gtgacattga gcgccagcat cacgcacagc gcgatgacca tcacgaacag gctgagccgc
      481 cggcggaaca gcgccagcag atagcgcagg tcaaccccgt cgccctccag gtggggcagg
      541 ccggccgacg gcagccgcgt gccatcgagc aaggccggca ggctgggggc gagatagcga
      601 tcgggcaggt tggtcgtggc catatgtgct ctcgaggtcg agtgactaca gggaaaaccg
      661 caggaacagg ccgccttcga cggcatggcc ctggcgcggg aggaggaacg aggcggactg
      721 acggtcgcgg tagcgggcgg tgaggcccag ctggacgtgc cggttggcgg cataggtggc
      781 ctcgatcccc gccgcattcg tgcggatcgt gggccaggcg ccgggcagat cgcggtaccg
      841 ctcaaccaca tgggcggcgg agaggacgag gttgaggttg cgcttcactt catagagcgc
      901 cttcagctcc acccggtcca ccaccacgcc cgcagcgccg ggcaggggcg aattgacgag
      961 gtcacgccgg ccggcgagcg tgaaggtgac cagcggggtg gcgtaccatt cgagccgcgc
     1021 gcgccacgcg aggccgcgat agttgcgcag ggccggcgag cgatagttcc gccactgcca
     1081 gccggcggcc agttcgccca tgacaaggtg gctgaattcg cccttcaggc cgaccagcgc
     1141 ttccccgacg cgacccgtgc gatcgggcgc ggtgccggga tggaggctgc gcacctgccg
     1201 caaggaggct tcggcaaagg cgatccgccc ggcgccgagc gagcgatcca cccgcagcga
     1261 gaggcctgtg gcccgctcat cgcggaaggc ctgactgatc atcgcgccgc cggggccaag
     1321 gacggggagg taatgcgtgc gggccagatc gaccgcgccg gtcagccggg tgcggccaag
     1381 atcaacccgc acgccgagac cggcctgcag ctggtcaacc agcaccacgc cgccatcgaa
     1441 gggattggcg ccggcggcct cgaacggagg ctccagccgc cgggccgcgg cgccggagag
     1501 ggccagggtg agcccgccgc cgaccggccg ggccgcctgc ccgcgcaggg cccaggtgtc
     1561 ggtgttgtac ccggaaagcc tggcgtagcg ggcaaggctg gcccgcccat cgaccacgaa
     1621 ccgggccgtg ccggtctggc cgacggccag caccgccggg gccacggttg cccagacatc
     1681 gccgcgcccg ccgccggacc ggttgaacac gttgctgctg gcttcgctgc gcacggtgag
     1741 cgagggggcg aggtgcatgc cgccgagatc gagccccggc gccgtatagc ccgggatctt
     1801 gcgcgcgccg gccacatcgt cgcgcaggag ggcgccctgt ggcccagcct cgaacggatc
     1861 gcggggcggg gccgactgcg ccagcgcgcg cccactcccc acgccggcgc cgagcgccag
     1921 caccagcccg cacaggcgcg ccgcccgctg cggatagggg cgccggatcg ggccggaggg
     1981 gagggtgacg gggttcacgg gaaggcgttc gctttcacaa ggcaagaggt tggggcggcc
     2041 gagcggggac aaaccggaat gaccgcaccg ggcggcgaag ttgtggtaaa tggcggggcg
     2101 cccttccggc gaactggtgc cgagaggacg gtgaacaggg caggagcaac aagtcaccag
     2161 ttcttccgat ccggtggggc cagagcggtt ccttgcagat gactgccgta tttcaccgga
     2221 taaggatgcg cgattcccgc ccgctaggga acgggggtac ccccggaact atatccggat
     2281 cggagggact tgctggagcg gccccgcgcg ccgctcgggg cacccggcgc gccggtcagt
     2341 gaaaggttcg tccgccgtct accgccagaa cggtgccagt aacgaaggag ctttcctcgc
     2401 tggtcaggaa caggcaggcc cgggcgatct cctcgggccg ggccacgcgc ttcagcgcat
     2461 attgccgtac gaagggcgca tcgtccggat tgtcgtagcc gcccagcacg tggctgacca
     2521 tcggggtgtc gacaatgccg ggggcgatgg agttgaccct gatcgccggg gccaactcgc
     2581 ccgcataggc cttcatcagc gcgattaccc cggccttgct ggcggcatag gtcgaaacgc
     2641 cggggccggt cggcagcagg ccggtgcccg aggcgatggt cacgatggtc gccgttccgg
     2701 cctggcgcag gtggggcagg gcggcctggc agaccaggaa gggcgcggtg aggttggtgt
     2761 cgatcacctt ggcccaggct tccagtgtcc attccccgag cgggtggctg gcggtgacac
     2821 cggcgcagtt gatcacgcca tcgagcccgc ccagcgccat ggcggcagcg ttgacggcag
     2881 ccgtggtcgc ggcgggatcg cacaggtcga ccggaatcgc gctggcgcca atgtccgtcg
     2941 caatctcggc cagcagcccg gcctgcacgt caagcagggc cagcgccgcg ccttctccgg
     3001 cgaacaggcg ggccgtggcg gcgccgatgc ccgatgcagc gccggtgatc aggatgcggc
     3061 gcccggccag gcggcctgtc gccccggtca caccgcgtcg cccagggcgc ggcgcgaggg
     3121 cttgagccca agccggaaga cgatcgcgcc gagcacggcc gtggtgaaga acatgatcgc
     3181 catcgacttg tggatcatca tcggatcgtg gaacacgtag tccgtaacca gggccacgcc
     3241 ggatgggccg agggtcatgc ccatcaggtt gtagctcagc gcgaacaggg ccgagacctg
     3301 accccggagc tcaggcgggg ttgccatttg caggtgggcc acggccggtc cggaaaaggg
     3361 cagcagcacg aagacgagcg cgtaaagtgg gacgaacacc cacaggctgg ttgacaggaa
     3421 ggcgatgggc gccacgacgg ccatgaacac gaagctgtag gcatagtagc gcaggtgcgc
     3481 atcgcgttgc ccggcggcga accagcggtc caccagccag ccgttgccgg catagccgag
     3541 caggccgatg gtagatccga tgatccccat cagcagaccg gcctgggcaa tgtcgaggcc
     3601 catgtgccgg gtgagcaggg ccggcgtcca ggccgcggtt ccgaaggcga ggacggcatt
     3661 gaggccgaaa ccgccgaaat ggcacaggaa gtacgtcggg ttcttgccga tgaaggccag
     3721 cagcagcccc ttgggcgagg ccttgcgttc gatcttgcgg cccttgcgca agtccggcac
     3781 cagccacggc aggaacgcga ccactgcacc gggcaagccc acgatgacga agaccacctg
     3841 ccaaggctgg agtgcgccga ccaggggcag ggtggcgccg ccgagctttt cgaaatggcg
     3901 gatgagcagg ccgccaccgg aataggcgag cgcgctgccg atagtcgagc cggtgccgaa
     3961 caggccgaag gccaggccaa gacgtcgctg cgggaaaata tcggccagca gcgaaaagac
     4021 cgcgggcaag agcgccgcct cgccgacccc cacggcaaac cttgccagcg cgagctgctg
     4081 aacgttttgc gagaggccgc agccaattgt cgctgccgac cacagcagca cgccgacagt
     4141 gatcaccgcg cgccgtgacg tgcgatccac cagccaaccg aacggcaacc cgaacacggc
     4201 atagaacagt ccgaaggcga cgccctggag aaggctgaac tggaagtccg tgatctccag
     4261 cgaactgcga atgggtccga ccaggagcga aaagatctgg cggtcgagat atcccaggat
     4321 cgtggccagc atgagcacga cgacactgaa ccacgccagg cggggggcca ccggccaact
     4381 gtcgatccgc gacggcgggg taacggaagc gtcgtcggcg gtcatcgggt ggtctcctgg
     4441 taacgggcga gaagttcgtg cttgcggacc ttgccgctgg gcaggcgcgg gatttccgcg
     4501 atgaaatcga ccgagcgcgg gcacttgtag tgggcaatcc gctcacggca gtaggcgatc
     4561 agttcggcgg ccagttcgtc agagggggcg acgccttccg cgagagcgac gacggccttc
     4621 acctcctcgc catattcggc gttcggcacg ccgatcaccg cgaggtcggc gatggcggga
     4681 tgcagcgtca gcacgttctc ggtttcctgg ggatagatgt tgactccgcc cgaaatgatc
     4741 atgttgtgct tgcggtcggc caggaacagg tagccctcgg catcgagata gccaacgtcg
     4801 ccggcggtgc accagatatc gtcgatgatg gttgcccgcg tcttgtccgg ctcgttatgg
     4861 tagaccatcg gggacgacag gcggaagtag atggtcccgg tctcgccttg cggcaggtcc
     4921 tcgcccccgt cgcccaggat ccggatgggg ccggcgatgg agcggccgac cgtgcccggc
     4981 cgctccagcc attcggcgga cgaaaccttg acgaatccgc cctcgctcgc gccgtaaaac
     5041 tcctcgatca ccgggcccaa ccagtcgatc atcgcgcgct tgacctcggg agggcagggg
     5101 gcggcggcat ggatcacggc cttcaggctc gagaggtcgt agcgcgcgcg aacctcctcc
     5161 ggcaacttca gcattcggac gaaatgggtc ggcacgaaca gggcatgggt cacccggtgc
     5221 tgctcgatcg cggcgagcac ggcttccggg tcaaagctgc gaaagacgac ggaggtgccg
     5281 cccatcacct gggtggcggt ggtccagctg atcggcgcgg cgtggtagag cgggccgggg
     5341 cagaggaaga ccgtttccgc gttgaagcca tagccccgcg tgatgaggtc ctcgcctggc
     5401 ccaggcgtac cggcgggatg gccgccgagg ggccggatga tccccttcgg cgcgccggtg
     5461 gtgccgtagg aatacatcat cgccgaccct tccacct
//
LOCUS       DATNBV010000315         2940 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3671387,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000315 DATNBV010000000
VERSION     DATNBV010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671387"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VN627_07540"
     CDS             <1..719
                     /locus_tag="VN627_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03146.1"
                     /translation="EQPAWAPAPVSSPVAGELSGEAAMRLCAAPLETLGVPQLVERFA
                     LALKARRLREADERASRGQRDPLPPAQPVTAPFAEPRDMPAHTGARPFDLPASHQTLM
                     DTGPLATRRPLSAAELPVDPTTELARWSLPEPEGEGDDVDEELWPDPAAAQDYSSLTD
                     LRPATRVPAPLADFVRVDEPDTVTGAGAQPVVVFPGQAVPAPPLPVRLPGTEAPANAE
                     ETEQALREALAALQRLSGTG"
     gene            974..>2940
                     /locus_tag="VN627_07545"
     CDS             974..>2940
                     /locus_tag="VN627_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HXD03147.1"
                     /translation="MGFPQAQGLYDPRNEHDACGVGFVAHIKGAKSHAIITQALEILK
                     NLDHRGAVGADPLLGDGAGILIQLPDQLLRSWAAGACVTLPQPGDYAVAMCFLPQDEA
                     SRALIVETFEKFIEKEGQTLVGWRDVPVTLDGLGKTVLESMPVIRQCVVGRGESCADQ
                     DAFERKLLAIRKQVQNPLAAMAEKHGLPGLTQLYMPSFSSRTIVYKGLLLATQVGSFY
                     DDLRNPEAVSALGLVHQRFSTNTFPSWKLAHPFRLIAHNGEINTVRGNVNWMNARRRT
                     MESDLLGPDLDKMWPIIPHGQSDTASLDNALELLLAGGYSLAHAVMMLIPEAWAGNPL
                     MDPKRRAFYEYHAALMEPWDGPAAVAFTDGRQIGATLDRNGLRPARFCVTDDDLVIMA
                     SESGVLPIKEDNIVRKWRLQPGKMLLIDFAEGRIIEDEEIKAQLANAEPYEEWLEAAQ
                     YNLKDLDVVEPELAQLPVETTSLLDRQQAFGYTQEDVSKFLEPMGVLADDPIGSMGTD
                     TPIAVLSNRSRLLYDYFKQNFAQVTNPPIDPIREELVMSLVSMIGPRPNLLGHDAGSH
                     KRLEVDQPILTNDDVAKIRSVEAALDGAFRTQTVDMTWDASTGAEGLEAAIKEMCWLA
                     TEAVLADKNILILSDRAQSPARVPMPALLATA"
BASE COUNT          510 a         1059 c          917 g          454 t
ORIGIN      
        1 cagaacaacc cgcatgggcc ccggcaccgg tttcgtcccc tgtcgccggc gaactgagcg
       61 gagaagcggc catgcggctg tgcgcggctc cgctggaaac actgggcgtg ccccaactgg
      121 tcgaacgctt tgcgctggcg ctcaaggccc ggcgcctccg cgaggcggat gaacgcgcca
      181 gccggggcca gcgcgatccg ctgccacctg cgcagccggt caccgctccc ttcgccgaac
      241 cgcgcgacat gccggcccac accggagccc gcccgttcga cctgcccgcc agccatcaga
      301 ctctaatgga taccggcccc ctggcgaccc gtcgcccgct ctcggcggcc gaactgccag
      361 tggatcccac gaccgaactg gcgcgctggt cactgcccga gcccgaaggc gagggtgacg
      421 atgtcgatga agagctgtgg ccggaccccg ccgccgcgca agactactcg tcgctgaccg
      481 atctgcgacc cgcgacccgc gtgcccgctc cgctcgccga cttcgtgcgg gtggatgagc
      541 cggacacggt gacaggtgcc ggggcgcagc cggtcgtcgt cttccccgga caggctgtgc
      601 cggcacctcc gcttccggtt cgtctgcccg gaaccgaggc cccggcgaac gccgaagaaa
      661 ccgaacaggc cctgcgcgaa gccctcgcgg ccctccagcg tctcagcggg actggctgat
      721 ccgccgacag caaggcggtg acaacggcac tttgtgccgt atggttccca acctcgtcgc
      781 gaaaaccggg cggatcgtgg ccgcggcagt acgcgcgcgc atgttcgcag ttgcaaaagc
      841 cccggcctga cgccattggc gcaggttgcg acactcgctg catttttgcg gccgggtgag
      901 tcgcgttttc gtgtccggca gacccttgga cctcaggggg ccgccgggag gctgttacag
      961 gatggattat tcgatgggat tcccgcaggc ccagggcctt tatgatccgc gcaacgaaca
     1021 cgacgcgtgc ggcgtcggct ttgtcgccca tatcaagggt gccaagagcc acgcgatcat
     1081 tacccaggct ctcgaaatcc tcaaaaacct cgaccatcgc ggcgctgtgg gcgctgaccc
     1141 gctgctgggc gatggtgccg gcatcctgat ccagctacct gaccagctcc tgcgctcgtg
     1201 ggccgccggc gcatgcgtca ccctgcccca gccgggcgac tatgccgtcg ccatgtgctt
     1261 cctgccgcag gacgaggcca gccgcgcgct gatcgtcgaa accttcgaga agttcatcga
     1321 gaaggaaggc cagaccctgg tcggctggcg cgacgtgccg gtcaccctcg acgggctcgg
     1381 caagaccgtg ctcgaatcta tgccggtgat ccgccagtgt gtcgtcggcc ggggcgagag
     1441 ctgtgccgac caggacgcgt tcgagcgcaa gctcctcgcc atccgcaagc aggtccagaa
     1501 cccgctcgcc gccatggccg aaaagcacgg cctgccgggg ctgacccagc tctacatgcc
     1561 gagcttctcc agccgcacca tcgtctacaa gggcctgctg ctggcgaccc aggtcggctc
     1621 tttctatgac gacctgcgca acccggaggc cgtttcggcc ctcggcctcg tgcaccagcg
     1681 cttttccacc aacaccttcc cgagctggaa gctggcgcat cccttccggc tgatcgccca
     1741 caatggcgag atcaacaccg ttcgcggcaa cgtgaactgg atgaacgccc gccgccgcac
     1801 gatggaatcg gacctcctcg gccccgatct cgacaagatg tggccgatca tcccgcacgg
     1861 ccagtcggac accgccagcc tggacaacgc gctggagctg ctgctggccg gcggctacag
     1921 ccttgcccat gcggtgatga tgctcatccc ggaagcctgg gccggcaacc cgctgatgga
     1981 tcccaagcgc cgcgcgtttt atgagtacca cgccgcgctg atggagccgt gggacggccc
     2041 ggccgccgtc gccttcacag atggccgcca gatcggcgcc acgctggacc gcaacgggct
     2101 gcggcctgcc cggttctgcg tgaccgacga cgatctcgtg atcatggctt cggaaagcgg
     2161 cgtcctgccg atcaaggaag acaacatcgt ccgcaagtgg cgcctccagc ccggcaagat
     2221 gctgctgatc gacttcgccg aaggccgcat catcgaggac gaggaaatca aggcccagct
     2281 cgccaatgcc gagccttatg aggaatggct ggaagccgcc cagtacaacc tcaaggacct
     2341 cgacgtggtc gagcccgaac tggcgcagct gccggtggag accaccagcc tgctcgatcg
     2401 ccagcaggcc ttcggctata cccaggaaga cgtctcgaag ttcctggaac cgatgggcgt
     2461 cctggccgat gatccgatcg gctcgatggg caccgacacc ccgatcgcgg tgctgtcgaa
     2521 ccgctcgcgc ctgctttacg actacttcaa gcagaacttc gcccaggtca ccaacccgcc
     2581 gatcgacccg atccgcgagg aactggtcat gtcgctggtg tcgatgatcg gcccgcgccc
     2641 gaacctgctc ggccacgatg ccggcagcca caagcggctc gaggtcgacc agccgatcct
     2701 gacgaacgac gacgtcgcca agatccgctc ggtcgaggcc gcgctcgatg gcgccttccg
     2761 cacgcagacc gtggacatga cctgggacgc cagcaccggc gccgaggggc tagaggccgc
     2821 gatcaaggaa atgtgctggc tggcgaccga agcggttctc gctgacaaga acatcctgat
     2881 cctgtccgac cgcgcccaat cgcccgcgcg cgtgcccatg ccggcactcc tcgccaccgc
//
LOCUS       DATNBV010000316         5804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1770351,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000316 DATNBV010000000
VERSION     DATNBV010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770351"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VN627_07550"
     CDS             <1..903
                     /locus_tag="VN627_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD03148.1"
                     /translation="GRVNQNGIFLLGPTLMEFGTHEQKQRFLPPMASGEEIWAQAWSE
                     PQAGSDLAGVRSTAVREGDDYVLNGHKIWSSRAAFADWAFGLFREPGSERHKGMSLIF
                     FRLDQEGVTRNPIRKINGHVGFAEIFLENVRVPAFNRLGEEGQGWHICMATAGFERGL
                     MLRSPARFQQAAAALAALWQSRKDTCDPLLEADVVRAHMNAEAYALNIYQTASRLMAG
                     AKIGPEASTNKIFWSEMDIHLHETALAILGAEAELLPPATSQAGAEHEWLDDYIFSLA
                     GPIYAGSNEIQRNIIAERMLGLPR"
     gene            918..1949
                     /locus_tag="VN627_07555"
     CDS             918..1949
                     /locus_tag="VN627_07555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD03149.1"
                     /translation="MDFTLTDEQQMFAETARTLLGDTCTPEHWRKLMERGQAFDASRW
                     AQIVENGFTLLLLPEAAGGLGLSELDLALIAIEAGYVALPEPLVESAGVGAPLLASLA
                     PDHPLLTDPAAIIAVAPAINPVVANADVAAAILIEQDGHAYLCAPETVTLTPAETIDP
                     LRRLFRVEWDESRAEDLGPANWDLALDRASLFAAAQGLGLAQRSVDLAIAYAKDRTQF
                     GKPIGSYQAVKHHLASAQTAIEFARPVVLAAAADIGHGDLQARARVSHAKIVALEAAE
                     KAARASIQVHGAMGYSWEVDVHLFLKRALALKAAWGTPAYHRNRIATRVFTQPLGADQ
                     TFARETEHA"
     gene            1942..3093
                     /locus_tag="VN627_07560"
     CDS             1942..3093
                     /locus_tag="VN627_07560"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXD03150.1"
                     /translation="MPEAYIVDAVRAPVGRKKGSLAQVHPADLAAHPIRELMARHDFD
                     PALVEDVVWGCTETIGGQAGDIGRTAWLVAGLAEDVPGVTIDRQCGSAQQAVHFAAQG
                     VMSGTQDIVVAGGSQAMNRIPIMAAMTAGAAYGFESPFIGAKGWDARYGDEEVNQIRS
                     AEMIAEKWAITREEMDAFALESHRRAQHATEQGWFRNEIVPLEGLDRDETIRPGTTLE
                     GLAALNPVREGGRVTAGNASQNCDCAAALLVVSERALKEHGLTPRARIHHMSVRADNP
                     VWMLTGPINATRYAMQKSGMKLDDIDLFECNEAFASVPMAWMKELGIPHEKVNVQGGA
                     IALGHPIGSTGARLMTTLLNALERTGGRYGLQTMCEGGGQANVTIIERL"
     gene            3109..3753
                     /locus_tag="VN627_07565"
     CDS             3109..3753
                     /locus_tag="VN627_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HXD03151.1"
                     /translation="MPCYAYQGIVPVVDPTSYVHPLASLIGDVIVGPGCFIAPGASLR
                     GDFGRIVIEGDSSIQDSATIHANQLRDTVIRRGATIAHGAIIHGCEIGENTLVGMNAV
                     VLDNAVIGEDNLVAALALVKSETITPPRSLVAGNPARVVHSFEPQQVTWRNEGQGEYQ
                     KLARAAVAGGIVATEPLAEVESNRRRIVSDAVAVRLSGPTAERRRKAAPPRGAQ"
     gene            3842..4753
                     /locus_tag="VN627_07570"
     CDS             3842..4753
                     /locus_tag="VN627_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD03152.1"
                     /translation="MSVGICDNRTVIITGAAGGLGRAYALAFGAEGANVVVNDIGTSL
                     GGEGRDTSAADAVVAEIKAAGGKAIANYEDITDWDAAKRIVDAAVEAFGDLHVVVNNA
                     GIVRDRMFVSATVEEWDATMHVHLRGHFCIARHAVNYWRAKQKDGRDPDARIINTSSG
                     AGLQGSIAQAAYSAAKGGIATLTLVQAAELARYGITANGLAPSARTRMTEGAFAEKMK
                     TVDEGFDVMDPANVAPTVVWLGSSQSAHVTGCMFELEGGKIMLEEGWREGPFVDQQAR
                     WEPSQIGEVVDKLIAQRVPPRKVWGTA"
     gene            4796..>5804
                     /locus_tag="VN627_07575"
     CDS             4796..>5804
                     /locus_tag="VN627_07575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD03153.1"
                     /translation="MEFAFSEEQAMIAETARAFFTENATSERTRMAMAATGPDAGIDR
                     ALWSAFCTELGLGGIGVPEELGGVGLGMVEFALVAEAAGAQVAALPLLGNARAVHALV
                     AGGTQEQKSRWIGGLAAGDVIATSASAAEAAAGPGTLSGTFRFAPHGGSADLLVLVAG
                     SRCWLVEAGAPGVTITSHVTMDQTRPLAMITLEQAPAEPVADHAAAIGALDTTGWICL
                     AAEALGGAQATLDRTVAYAQERVQFGRQIGSFQAYKHRLADMMIAIEQARSAVYWAAC
                     AVDEGSAEAAIALHAAKAFCADTFHMCAGNMIQLHGGIGFTWEHDAHLFFKRARSIAS
                     ML"
BASE COUNT          962 a         1938 c         2002 g          902 t
ORIGIN      
        1 gggcgggtca accagaacgg gattttcctg ctcggcccca cgctgatgga attcggcacg
       61 cacgagcaga agcagcgctt cctgccgccg atggccagcg gcgaggagat ctgggcccag
      121 gcctggtccg agccgcaggc cgggtccgac cttgccgggg ttcgctcgac cgctgtgcgc
      181 gagggcgatg attacgtgct caatggccac aagatctggt cgagccgcgc ggccttcgcc
      241 gactgggcct tcggcctgtt ccgtgagccg ggcagcgagc gccacaaggg catgagcctg
      301 atcttcttcc ggctcgatca ggagggcgtt acccgcaacc cgatccgcaa gatcaacggt
      361 cacgtcggct tcgccgagat cttcctcgaa aacgtgcgcg tccccgcctt caaccggctg
      421 ggcgaggaag ggcagggctg gcacatctgc atggcgacgg ccgggttcga gcgcgggctg
      481 atgctgcgca gcccggcgcg cttccagcag gcggctgccg cgctggcggc gctgtggcag
      541 agccgcaagg acacttgcga cccgttgctc gaggccgacg tggttcgcgc gcacatgaat
      601 gccgaagcct atgcgctgaa catctaccag acagcgtcgc ggctgatggc cggggcgaag
      661 atcgggcccg aagcctcgac caacaagatt ttctggtcgg agatggacat ccacctccac
      721 gaaaccgcgc tggcgatcct cggcgccgag gccgagttgc tcccgcccgc cactagccag
      781 gcgggcgccg agcacgagtg gctcgacgac tacatcttct cgctggccgg gccgatctat
      841 gccgggtcga acgagatcca gcgcaacatc atcgccgagc gcatgctcgg cctgccgcgc
      901 tgaacggggg gcaggacatg gatttcaccc tcaccgatga gcagcagatg ttcgccgaga
      961 ccgcgcgaac gctgctgggt gatacctgca cgccggaaca ttggcgcaag ctgatggaac
     1021 ggggccaggc attcgacgcc tcgcgctggg cgcagatcgt cgagaacggc tttaccctcc
     1081 tgctcctgcc cgaggccgcc ggaggcttgg gcctgagcga gctcgatctt gccctgatcg
     1141 cgatcgaggc gggctatgtt gccttgcccg agccgctcgt ggagagcgcc ggtgtgggcg
     1201 cgccgctgct ggcgagcctt gcgcccgatc atccgttgct gaccgatccg gccgcgatca
     1261 tcgcggtcgc tcctgccatc aacccggtcg tcgccaatgc cgacgtcgcc gccgccatcc
     1321 tgatcgagca ggacggccat gcctacctgt gcgcgcccga aacggtgacg ctgaccccgg
     1381 ccgaaaccat cgatccgctc cgccgcctgt tccgggtgga atgggacgag tctcgggccg
     1441 aggatctggg tccggccaac tgggacctgg cgctggaccg ggcgtcgctg ttcgctgcgg
     1501 cgcagggcct cggcctcgcc cagcggtcgg tcgatctcgc cattgcctat gcgaaggacc
     1561 gcacccagtt cggcaagccg atcggatcct atcaggcggt caagcatcac cttgccagcg
     1621 cccagacggc gatcgagttt gcccggccgg tggtgctcgc cgccgccgcg gacatcgggc
     1681 atggtgactt gcaggcccgc gcccgtgtgt cccatgccaa gatcgtcgcc cttgaagcgg
     1741 ccgagaaggc cgcgcgtgca tcgatccagg tgcatggcgc gatgggctat tcctgggagg
     1801 tcgacgtcca cctgttcctg aagcgcgcgc tggcgctgaa ggcggcctgg ggcactcccg
     1861 cataccaccg caaccgcatt gcgacgcgcg tcttcaccca gccgctcggc gccgaccaga
     1921 cctttgcccg cgagacagaa catgcctgaa gcctatatcg ttgatgccgt tcgcgctccc
     1981 gtcgggcgca agaagggcag ccttgcccag gtccatccgg ccgacctggc tgcgcacccg
     2041 atccgcgaac tgatggcgcg ccatgacttt gatcccgcgc tggtcgaaga cgtggtctgg
     2101 ggctgcaccg agaccatcgg cggccaggcc ggggatatcg gccgcaccgc ctggctcgtc
     2161 gccgggctgg ccgaggacgt tccgggcgtg acgatcgacc gacagtgcgg ttcggcacag
     2221 caggcggtcc acttcgccgc acagggcgtg atgagcggaa cgcaggatat cgtcgtcgcc
     2281 ggcggcagcc aggcgatgaa ccgcatcccg atcatggccg cgatgaccgc tggcgccgcc
     2341 tacgggttcg agagcccctt catcggcgcc aagggctggg acgcgcgcta tggggacgag
     2401 gaggtcaacc agatccgttc ggccgagatg atcgccgaaa agtgggccat cacccgcgag
     2461 gagatggacg ccttcgcgct ggaaagtcac cgccgcgccc agcatgcgac cgagcagggc
     2521 tggttcagga acgagatcgt gccgctcgaa gggctcgacc gcgacgagac gatccgccct
     2581 ggcaccacac tggagggcct ggccgcgctc aaccccgtcc gcgaaggggg ccgggtcacg
     2641 gcgggcaatg ccagccagaa ttgcgactgc gccgcggcgc tgctggtcgt cagcgagcgg
     2701 gcgctgaagg agcatggcct cacgccgcga gcgcgcatcc accacatgtc ggtgcgcgcc
     2761 gataacccgg tgtggatgct caccgggccg atcaacgcca cccgctatgc catgcagaag
     2821 tccggcatga agctggacga tatagatctg ttcgaatgca acgaggcctt tgcctcggtg
     2881 ccgatggcgt ggatgaagga actcggcatt ccgcacgaga aggtcaacgt gcagggcggg
     2941 gccatagcgc ttggccaccc gatcgggtcg accggcgcca ggctgatgac caccctgctc
     3001 aatgcgctgg agcgcacggg cggccgctac ggcctgcaga cgatgtgcga gggcggcggc
     3061 caggccaacg tcaccatcat cgagcggctc tgaggatcgg tccgcgccat gccatgctat
     3121 gcctaccagg gaatcgtgcc ggtcgttgat ccgaccagct atgtccatcc cctggcatcc
     3181 ctgatcggtg acgtcatcgt ggggccaggg tgcttcatcg cgcccggggc ttcgttgcgc
     3241 ggcgatttcg gccggatcgt gatcgagggc gacagttcga tccaggacag tgcgaccatc
     3301 cacgccaacc agttgcgtga tacggtgatc cggcgaggcg cgacgatcgc ccacggcgcg
     3361 atcatccatg gctgcgaaat cggcgagaat acgctggtcg gcatgaatgc cgttgtgctc
     3421 gataacgcgg tgatagggga ggacaacctc gttgccgcgc tggcgctggt gaagtccgag
     3481 acgatcacgc cgccgcgcag cctggtggcc ggcaacccgg ccagggtcgt gcacagtttc
     3541 gaaccgcagc aggtgacgtg gcgcaacgaa gggcaaggcg aataccagaa gcttgcccgc
     3601 gccgctgttg ccggcggcat cgtggcgacc gagccactgg ccgaagtcga gagcaaccgt
     3661 cgccgcatcg tttccgacgc cgtcgcggtg cgcctgtccg gcccgactgc cgagcggcgc
     3721 cggaaggcgg cgccgccgcg aggtgcacag tgacaccgct tgggaccatt cagaacccga
     3781 tttcaccggc gacccttcgc agctgctgct gcctgcggcc ggccgagaca tcccaaggag
     3841 aatgagcgtg ggcatttgcg acaatcgcac cgtcatcatc accggcgccg ccggcgggct
     3901 tggccgcgcc tatgccctgg ccttcggggc cgaaggcgca aacgtcgtgg tcaatgacat
     3961 cggcacttcg ctcggcgggg aagggcgcga caccagcgcc gccgacgccg tggtggcaga
     4021 gatcaaggcg gccggcggca aggccatcgc caactatgag gacatcaccg actgggatgc
     4081 cgccaagcgc atcgtcgatg cggcggtcga ggcctttggt gacctccacg ttgtggtgaa
     4141 caatgccggc atcgtgcggg accggatgtt cgtttcggcg acggtcgagg aatgggacgc
     4201 gaccatgcac gtccacctgc gcgggcattt ctgcattgct cgccatgccg tcaactactg
     4261 gcgcgccaag cagaaggacg ggcgcgatcc cgatgcgcgg atcatcaata cctcgagcgg
     4321 cgcggggctg cagggttcga tcgcgcaggc agcctattcc gcggcgaagg gcgggatcgc
     4381 gacgctgacc ctggtccagg ccgccgagct cgcccgctat ggcatcaccg ccaacggtct
     4441 ggccccatcg gcccgcaccc gcatgaccga gggcgccttt gccgagaaga tgaagacggt
     4501 cgacgaaggc ttcgacgtca tggacccggc caatgtcgcg ccgacggtcg tctggctggg
     4561 cagcagccag agcgcccatg tgaccggctg catgttcgag ctggaaggcg gcaagatcat
     4621 gctcgaggaa ggttggcgcg aagggccgtt tgtggaccag caggcccgct gggaaccgag
     4681 ccagatcggt gaggtggtcg acaagctgat cgcccagcgc gtgccgccgc gcaaggtctg
     4741 gggcacggcg tgagcccgcc tcgctgcagc tcgccgcatt cgaggatgtg acctgatgga
     4801 attcgccttt tccgaagaac aggccatgat cgcggaaact gcgcgggcct tcttcaccga
     4861 aaacgcgacg agcgagcgca cccgcatggc gatggcggcg accggcccgg acgccgggat
     4921 cgaccgggcg ttgtggtccg ccttctgcac cgagctgggc cttggcggga tcggcgtacc
     4981 ggaagagctg ggcggggtcg ggctgggcat ggtcgagttc gcgctggtcg ccgaggcggc
     5041 gggcgcgcag gtcgccgcct tgccgctgct cggcaatgcc cgggcggtgc atgcgctggt
     5101 tgccggcgga acgcaggagc agaagtcgcg ctggatcggc gggctggccg cgggcgatgt
     5161 cattgccacc tcggcctcgg ctgcagaggc cgcggccggg ccgggcacgc tttccggcac
     5221 cttccgattt gccccgcatg gcggcagcgc cgacctattg gtgctggttg caggcagccg
     5281 gtgctggttg gtcgaagccg gcgcgccggg cgtcacgatc acgtctcacg taaccatgga
     5341 ccaaacccgg ccactggcga tgatcacgct cgagcaggca cccgccgaac ccgtggccga
     5401 ccacgccgcc gcgatcggcg cgctcgacac taccggctgg atctgcctcg ccgccgaggc
     5461 cctgggcggg gcgcaggcga cgctcgaccg caccgttgcc tatgcgcagg agcgggtcca
     5521 gttcggccgc cagatcggct cgttccaggc ctacaagcat cgcctggccg acatgatgat
     5581 cgcgatcgag caggcgcgtt cggcggtcta ctgggcggcc tgcgcggtcg atgaaggcag
     5641 cgccgaggcc gccatcgcgc ttcacgcagc caaggcgttc tgcgccgata ccttccacat
     5701 gtgcgccggc aacatgatcc agctgcatgg cggcatcggc ttcacctggg agcacgatgc
     5761 ccacctcttc ttcaagcggg cgcggtcgat cgcttccatg ctgg
//
LOCUS       DATNBV010000317         5018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_824902,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000317 DATNBV010000000
VERSION     DATNBV010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824902"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VN627_07580"
     CDS             complement(<1..1142)
                     /locus_tag="VN627_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXD03154.1"
                     /translation="MSDTPENTSGANTPNQNAYGAESIKVLKGLDAVRKRPGMYIGDT
                     DDGSGLHHMVFEVSDNAIDEALAGHCDLVLIELNPDGSVSVEDNGRGIPTGIHPEEGV
                     SAAEVIMTQLHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLELTIWRDGQEHW
                     MKFAHGDAVESLRVVGPAPDNGKGAPKKGTRVTFLASDETFKNVTVYDFEKLEHRYRE
                     LAFLNSGVRILLRDKRGEEVKEHDLYYEGGIAAFVKWLDRNKQALLPDPIAISAQRDG
                     IGIDVALEWNDSYYENVLCFTNNIPQRDGGTHLAAFRAALTRTLNNYAEKSGMLKKEK
                     VSLTGDDMREGLTAIVSVKMPDPKFSSQTKDKLVSSEVRQPLESLMG"
     gene            1297..3417
                     /locus_tag="VN627_07585"
     CDS             1297..3417
                     /locus_tag="VN627_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXD03155.1"
                     /translation="MRRIVPLACSLGALLVAVPALADAGAGASAAADAPGADPHDHTP
                     PEIVVTGAIRQNRLDALSGVAVIQGAKLDQAIRPSIGETLAHTPGVSSTSFGPTASRP
                     VLRGMQGERVRLLTDGIGSIDVSNTSADHAAVVNPLLAERIEVLRGPQSLLYGSSAIG
                     GVVNVIDRRIPITVPSEAVHFGGVAGYGTAAHERSLAGSVEAPLGGGFVAHADGSWDK
                     SDDLRIGGYALTPALRAQALATAASGLGDPDIDYAGNAAVAGVLPNTAARTWTAGAGL
                     SYINDGGMLGASYTHSDSLYGVPIRFATVPGEGQEAPRLAMKQDRLDLRAEIKPGGAI
                     DHVLLRAAYADYRHSELGPDGSVGTTFLDKGIEARLEVAQAKLGAWQGASGVQFVNRD
                     FNVIGDEAFLPKNGTQSFGLFTVQQLDYGELKLEAGARFEHARITAQPLASGPQFFSG
                     ERRFDTFSGSIGASYAVLPGWRLGLNVSRTARAPAAEELFANGPHAGTEAYEIGDPNF
                     KVERATSLEATFKGGDADLGLEASAYYTWFGNFIYDARTGATIDGLPVYQGRQGDARW
                     YGFEVEGHAALARFGGWKLAADGMADAVRATIANYGPAPRIPPLRLLGGLEATSARVD
                     LRGEVEHVTAQNRVAPNETVTSGYTMVNLSAAWRPWGEDRPLSFTLSANNLFDVVARR
                     HASFLKDYAPLAGRDIRLTARVEL"
     gene            complement(3472..3993)
                     /locus_tag="VN627_07590"
     CDS             complement(3472..3993)
                     /locus_tag="VN627_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HXD03156.1"
                     /translation="MPETTPPEPLRKQNHPRAQAAREKRRAEAEAAGVDDAFISALVD
                     RFYQRIRGDALLGPIFARHIADWPPHLARMKAFWRSILHNSGEFSGNPMQRHTALHDA
                     GSPLGEAEFAHWLGLFYATCAELAAERGLSPETARQVGQRARMIADSLLTGIATREHG
                     LTAARAGDGLPRP"
     gene            complement(4116..>5018)
                     /locus_tag="VN627_07595"
     CDS             complement(4116..>5018)
                     /locus_tag="VN627_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXD03157.1"
                     /translation="TRFDAARAQLAPAIGRVPSGDPLTVQLASLAASSQGRIGVAALD
                     LATGRSVGVLGDQSFPMASTSKVAIVATFLAGVDQGRWQLTDRFPLMVPVPSAKFSSA
                     VAPVRPGTELTALQLIELALTRSDNHATDGLLAVVGGPQAVTRWVHQNTGLTEFRLDR
                     DIATLVRDDGAINPAITVDKRDSITPNAMVRLLAGLYQGQWLSASSRQVLLGAMSRCI
                     TGKARMRALLPEGAAIGHKTGTLFNTASDVGFIETPDGRKLAVAIYVTGQGGKPQRNS
                     RIALLARTIYDGYRADGTRQAALR"
BASE COUNT          739 a         1564 c         1871 g          844 t
ORIGIN      
        1 cccatcaggc tttcgagcgg ctggcggact tcgctcgaga ccagcttgtc cttggtctgc
       61 gaggagaact tcgggtcagg catcttgacc gagacgatcg cggtgaggcc ctcacgcatg
      121 tcgtcgccgg tgagcgaaac cttctccttc ttcaacatgc ccgacttctc ggcatagttg
      181 ttgagggtcc gggtcagcgc ggcgcggaag gcggcgaggt gagtgccgcc atcgcgctgg
      241 gggatgttgt tggtgaagca caggacgttt tcgtagtacg aatcgttcca ttccagcgcg
      301 acgtcgatgc cgatgccatc gcgctgggcg ctgattgcga tgggatcggg gagcagcgcc
      361 tgcttgttgc gatcgagcca cttcacgaag gcggcgatcc cgccttcgta atagagatca
      421 tgctccttca cttcctcgcc ccgcttgtcg cgcaggagga tacgcacgcc ggagttgagg
      481 aaggcgagct cgcgatagcg gtgttcgagc ttttcgaaat cgtagacggt gacgttcttg
      541 aaggtctcgt ccgaggcgag gaaggtgacg cgggtgccct tcttgggcgc gcccttgccg
      601 ttgtcgggcg cggggccgac gacgcgcagc gattccaccg catcgccgtg ggcgaacttc
      661 atccagtgct cctggccgtc gcgccagatg gtgagttcga gccattccga cagggcgttg
      721 accaccgaaa cgccgacgcc gtggaggccg ccggagacct tgtaggcatt gtcgtccgaa
      781 gtgttctcga acttgccgcc ggcgtggagc tgggtcatga tgacctcggc cgccgagacg
      841 ccttcttccg ggtgaatgcc ggtggggatg ccgcggccgt tgtcttccac gctgaccgac
      901 ccatcggggt tgagttcgat gaggacgagg tcgcaatggc cggcgagagc ctcgtcaatc
      961 gcgttgtccg aaacctcgaa gaccatatgg tgcaggcccg agccatcgtc ggtatcgccg
     1021 atgtacatgc cggggcgctt gcgcacggca tcaagcccct tgagcacctt gatggattcg
     1081 gcgccgtagg cgttctggtt cggggtgttg gcgccggaag tgttttcagg ggtgtcggac
     1141 atggcaagca tatagggact cgcgcgcgcg taaggaagcg aaatggccct cccgtccaca
     1201 ggaatggggc catttcgcgc cgatgtgatg tgatattgta tccttcgctg cgctgcggta
     1261 atgctgcgcc acctggggcc tcaggagacc tgccgaatgc gccgcatcgt tccccttgcc
     1321 tgcagcttgg gcgcccttct cgtcgccgtt cccgcgctgg cggacgctgg cgcaggcgct
     1381 tccgccgcgg ccgatgcgcc cggggccgat ccgcacgacc acactccccc ggagatcgtc
     1441 gtcaccggcg cgatccggca gaaccggctg gatgccctct ccggcgtggc ggtgatccag
     1501 ggggcaaagc tggatcaggc gatccgcccc tcgatcggcg agaccctggc ccatacgccg
     1561 ggtgtttcct cgacctcgtt cggccccact gcctcgcgcc ccgtgctgcg cggaatgcag
     1621 ggggagcggg tgcggctgct gaccgacggg atcggctcga tcgacgtttc gaacacctcg
     1681 gccgaccatg ccgccgtggt gaaccccttg ctggccgagc ggatcgaggt gctgcgcggg
     1741 ccgcagtcgc tgctttatgg atcgagcgcc attggcggcg tggtcaacgt gatcgaccgg
     1801 cgcattccga tcacggtgcc cagcgaggcc gtgcatttcg gcggggtggc gggttatggc
     1861 acggcggcgc acgagcggtc gctcgccgga tcggtggaag cgccgctggg ggggggcttt
     1921 gtggcccatg ccgatggctc gtgggacaag tcggacgacc tcaggatcgg cggctatgcg
     1981 ttgaccccgg cgctgcgggc ccaggcgctc gcgacagccg cttcggggct gggcgatccc
     2041 gacatcgact atgccggcaa tgccgcggtg gcgggcgtcc tgcccaacac ggcggcgcgg
     2101 acatggaccg cgggcgccgg gctgtcctac atcaacgacg gcggcatgct tggggcaagc
     2161 tatacccaca gcgacagcct ttatggcgtg ccgatccgct tcgcgaccgt gccgggcgag
     2221 ggccaggagg cgccgcgcct cgcgatgaag caggaccggc tggacctgcg cgccgagatc
     2281 aagcccggcg gggcgatcga ccacgtgctg ctgcgcgcgg cctatgccga ctatcgccat
     2341 tccgaactcg ggccggacgg cagcgtggga acgaccttcc tcgacaaggg gatcgaggcg
     2401 cggctggaag tggcccaggc aaagctcggc gcgtggcagg gggcgagcgg ggtgcagttc
     2461 gtcaaccgcg atttcaacgt gatcggcgac gaggccttcc tgccgaagaa cgggacccag
     2521 tcgtttggcc tgttcaccgt ccagcagctc gattacggcg agctcaagct ggaggcgggg
     2581 gcacgcttcg agcatgcccg gattacggcg cagccgctgg caagcgggcc gcagttcttt
     2641 tcgggcgagc ggcggttcga taccttttcc ggctcgatcg gcgcgagcta tgccgtgctg
     2701 cccgggtggc ggctggggct gaacgtctcg cgcaccgccc gggctccggc ggcggaagag
     2761 ctgttcgcca atggccccca tgccgggacc gaggcctacg agatcggcga tcccaacttc
     2821 aaggtggagc gggcgaccag ccttgaggcg accttcaagg gtggcgatgc cgatctcggg
     2881 ctggaggcat cggcctatta cacctggttc ggcaacttca tctacgatgc gcggaccggc
     2941 gccacgatcg acggcctgcc ggtttaccag ggccggcagg gcgatgcccg ctggtatggc
     3001 ttcgaggtgg aggggcatgc ggcccttgcc cgctttggcg gctggaagct cgcggccgac
     3061 ggcatggccg atgcggtgcg ggcgacgatc gccaattatg gtcccgcccc gcgcatcccg
     3121 ccgctgcgcc tgcttggcgg cctggaagcg accagcgcca gggtggacct gcgcggcgag
     3181 gtggaacacg tcaccgccca gaaccgcgtg gcgcccaatg agacggtgac gtcgggctat
     3241 accatggtca acctcagcgc ggcctggcgc ccgtggggcg aggaccggcc gctgagcttc
     3301 acgctatcgg ccaacaacct gttcgatgta gtggcgcggc ggcacgcctc gttcctcaag
     3361 gactacgcgc cgctcgccgg gcgggacatc cgcctgacgg cgcgggtcga gctttagggg
     3421 taggcgacca gggctcctct gccgctatcg tcggaggcgc cggttggggc gttacggccg
     3481 gggcaggcca tcgcctgccc gcgccgccgt cagcccgtgt tcgcgagtgg cgatgccggt
     3541 gagcaggctg tcggcgatca tccgcgcgcg ctggccgacc tggcgagccg tttcggggga
     3601 gaggccgcgc tcggcggcga gttcggcgca ggtggcgtag aacaggccca gccagtgggc
     3661 gaattcggcc tcgcccagcg ggctgccggc atcgtggagc gcggtatgcc gctgcatcgg
     3721 gttgccggag aactcgcccg aattgtgcag gatcgagcgc cagaacgctt tcatccgggc
     3781 gaggtggggc ggccagtcgg caatgtggcg ggcgaagatg gggccgagca gggcatcgcc
     3841 gcgaatgcgt tgatagaagc ggtcgacaag ggcggagatg aacgcgtcgt cgaccccggc
     3901 ggcctcggcc tcggcgcggc gcttttcacg ggcggcctgg gcccgcgggt ggttctgctt
     3961 gcgcagcggt tcgggcgggg tggtttcggg catgacggtg acgtggggcc tgggcgcggc
     4021 cggtgcaagg gtccgtgcga ggcaggccgc ttggctccta accgccccgt cagcgcagtg
     4081 cgctgccacc tccccacgcg tggggaggat ttggcttagc gcagggcggc ctggcgggtg
     4141 ccgtcggcgc gatagccgtc gtagatggtg cgggcgagga gggcgatgcg tgagttgcgc
     4201 tggggcttgc cgccctggcc ggtgacgtag atcgccacgg cgagcttgcg gccatcgggc
     4261 gtctcgatga agccgacgtc gctggcggtg ttgaacaggg tcccggtctt gtggccgatc
     4321 gccgcgcctt cgggcagcag ggcgcgcatg cgggccttgc cggtgatgca gcggctcatc
     4381 gcgccgagca ggacctggcg gctggaggcg ctgagccact ggccctggta caacccggcg
     4441 agcaggcgga ccatggcatt gggggtgatg ctgtcgcgct tgtcgacagt gatggcggga
     4501 ttgatcgcgc catcgtcgcg cacgagagtg gcgatgtcac ggtccagacg gaactcggtc
     4561 aggccggtgt tctggtggac ccagcgcgtg acggcctgcg gaccacccac cacggcaagc
     4621 aggccgtcgg tggcgtgatt gtcgctgcgg gtaagggcga gctcgatcag ctggagcgcg
     4681 gtcagttccg tgccggggcg gaccggggcg acggcgctgg agaacttggc cgacgggacc
     4741 gggaccatca gcgggaaccg gtcggtcaac tgccagcggc cctggtcgac cccggccaag
     4801 aaggttgcga caatggcgac cttgctggtt gaggccatgg ggaaggattg gtcacccagg
     4861 acgccgaccg agcggccggt ggcgagatcg agcgcggcga cgccgatccg tccctgcgaa
     4921 ctggcggcga gcgaggcgag ctggacagtg agcggatcgc cggacggcac gcgcccgatt
     4981 gccggggcga gctgggcgcg ggcggcatcg aagcgggt
//
LOCUS       DATNBV010000318         2736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_825265,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000318 DATNBV010000000
VERSION     DATNBV010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825265"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VN627_07600"
     CDS             <1..339
                     /locus_tag="VN627_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HXD03158.1"
                     /translation="PLRVRGHAFRVEIARTDAEQQRGLMYRTIMGANEGMLFPEALPR
                     RVAFWMRNTVLPLDIVFIGTDRRILNIVSAIPYDETPLPSAGPVSAVLELNAGRTRQL
                     RIRPGDKVIW"
     gene            408..821
                     /locus_tag="VN627_07605"
     CDS             408..821
                     /locus_tag="VN627_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HXD03159.1"
                     /translation="MGILSKIFTWWDGATIGTALDSARHGEHVGTDAQGNRYYRARRP
                     FPKGHPRAGLERRWVIYNGANDASRVPAEWHGWLHSTFDGVPESYLPPPRIWEADYTP
                     NATGTPQAYRPQGALENGGIRARATGDYEAWSPEA"
     gene            715..1245
                     /locus_tag="VN627_07610"
                     /pseudo
     CDS             715..1245
                     /locus_tag="VN627_07610"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
     gene            complement(1281..2051)
                     /gene="aat"
                     /locus_tag="VN627_07615"
     CDS             complement(1281..2051)
                     /gene="aat"
                     /locus_tag="VN627_07615"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HXD03160.1"
                     /translation="MLAYRSGIFPMADSRDDPEVFWVEPRLRAILPLDGFRCSRSLAR
                     TLRRGRFTVTCNAAFTAVMEACAAPRRESEETWISERIASSYTALHYEGFAHSIECWQ
                     DEDGDGSRRRLVGGLYGVGFDRVFCGESMFSVVPDASKVALAWLVAALREAGAELLDC
                     QFMTEHLASLGAIEISQARYLTLLKRAQEPRPRDQSGWAGAAGAAAGAGAGEGLAEGA
                     GTGAPLALPAGFAALLAKAAGSSSSPGNFIAQSFTQTS"
     gene            2220..2295
                     /locus_tag="VN627_07620"
     tRNA            2220..2295
                     /locus_tag="VN627_07620"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2253..2255,aa:Trp,seq:cca)
BASE COUNT          464 a          940 c          914 g          418 t
ORIGIN      
        1 ccgctgcgcg tgcgcggcca tgccttccgg gtcgagatcg cccgcaccga tgccgagcag
       61 cagaggggcc tgatgtaccg gaccatcatg ggcgcgaacg agggcatgct gttccccgaa
      121 gccttgcccc gccgcgtggc cttctggatg cgcaatacgg tgctgccgct ggatatcgtg
      181 ttcatcggga cggaccggcg gattctcaac atcgtcagcg ccattcccta tgacgagacc
      241 ccgctgcctt cggccgggcc ggtgtcggcg gtgctggagc tgaatgcggg gcggaccagg
      301 cagttgcgga tcaggccggg ggacaaggtg atctggtaac aatcgcgcgt gacgaggctg
      361 ccaaccccct tgcccgatcc cccctgtttc cgctaggcgc gaggcccatg ggaatcctct
      421 ccaagatctt cacctggtgg gacggcgcca ccatcggcac cgcgctcgat tcggcgcgcc
      481 atggcgagca tgtcggcacc gatgcgcagg gcaaccgcta ctaccgtgcc aggcgcccgt
      541 tccccaaggg ccatccgcgc gccgggctgg agcgccgctg ggttatctac aacggcgcca
      601 acgatgccag ccgcgtgccg gccgaatggc acggctggct ccacagcacc ttcgatggcg
      661 tgccggaaag ctaccttccg cccccgcgca tctgggaggc ggactatact cccaatgcca
      721 ccggcacccc gcaggcctat cgcccccagg gcgcgctgga gaacggcggc attcgcgccc
      781 gcgccaccgg cgactacgaa gcctggtcgc cggaagcctg accgggtgcg cccgcactcg
      841 gccctgatcc gctccattac cgtcgttgcg gcgagcctcg cggccgcggc ctgcgggcat
      901 ggcggcgacg tggcgccaga acccaccgaa gttccgcaga acgttgccgg cgcggcgccg
      961 gcggcgcggg cggtggagag caattacggc accccggtga aggaccgggt ggccacgatc
     1021 ggcctgctca acaagcgcaa caacctcgag caggacctcg tgctgaagac cggcgaagcc
     1081 cggcggatcg gcaatgtcgt gatcaagctc gccaactgcg agcggaccct gccgtgggag
     1141 gacccgccgg aagtgggcgc tttcgtccag ctctgggtcg aggaacgcgc cgacgtgtcc
     1201 aagccgctgg cctggcacaa ggtgttttcc ggctggctgt tcaagaactc gccctcgctc
     1261 aacgtggtcg agcacccggt ctacgatgtc tgggtgaagg actgcgcgat gaagttcccg
     1321 ggcgaggagg aggaccccgc cgctttcgcc agcagcgcgg cgaaacccgc gggcaaggcg
     1381 agcggcgccc cggtaccggc accttcggcc agcccctctc cggccccggc gcctgccgcg
     1441 gcacccgcag cgccagccca gccggactga tcccgcggcc ggggctcctg cgcccgcttg
     1501 agcagggtca ggtagcgcgc ctgcgagatc tcgatggcgc ccaatgaggc gaggtgttcg
     1561 gtcatgaact ggcagtcgag cagttcggcc ccggcctcgc gcagggcggc gaccagccag
     1621 gcgagggcga ccttggaggc atcgggcacc acggaaaaca tcgactcgcc gcagaacacc
     1681 cggtcgaacc ccaccccgta gagcccgccg accaggcggc gcctgctgcc gtcgccatcc
     1741 tcgtcctgcc agcattcgat cgagtgagcg aagccctcgt aatggagcgc ggtgtagctg
     1801 gaggcgatcc gctcgctgat ccaggtttcc tcgctctccc ggcgcggggc ggcgcaggct
     1861 tccatcaccg cggtgaaggc ggcgttgcag gtgacggtga agcgcccccg gcgcagggtg
     1921 cgggcaagcg aacgcgagca gcggaaaccg tcaagcggga gaatggcccg gaggcgcggt
     1981 tccacccaga acacctcggg atcgtcgcgg ctgtccgcca tcgggaagat cccgctgcgg
     2041 taggccagca tcaggagctg gggatcgatg atcttgcccg gggcgtgcac cggcgcagtc
     2101 tagcgcgaat ccggctttgt cgcaaaagcc cttgcccgcc cgaccggaca ggtcgcgcgg
     2161 gcacgcgacg agcagccttg ccatccggcc gcgcggccta tagaggcccc cctgcccgca
     2221 ggagtgtagc tcagttggta gagcatcggt ctccaaaacc gagggccggg ggttcgagtc
     2281 cctccactcc tgccaaaaat ctgccggaaa cccgcaaaat tgtgcgggtt atcggccagg
     2341 caacacggtg cccctcctcc cttggtagcc aagcgccagc tgtcacgagg ccgagcgaga
     2401 aaagcggacc gttcggcatc gggaagatgc cagcatacag caacgagatt ggtccgaaga
     2461 tcggcgcgtt ccccccttga ccggaacacc cgcgaggcgt ggtctgacca tctcagcttg
     2521 cggctatcgc gctgatcggc aaggtttcgg cgctgggcag gggttcatgt gggcgatgac
     2581 tgccgctagg atgctcgtcg tttctcagct gaccgcatcc ggaatttatc atgacgatca
     2641 agaaattagc cctcgcaacg ctgcttttga gctgctcggt ctccgtatct gctcagacag
     2701 tcgctgacga cgtgcgctgc ctgatgctca gcaatc
//
LOCUS       DATNBV010000319         3186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1469134,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000319 DATNBV010000000
VERSION     DATNBV010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469134"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..793)
                     /locus_tag="VN627_07625"
     CDS             complement(5..793)
                     /locus_tag="VN627_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXD03161.1"
                     /translation="MDYNTLLVDQEDGGIVWITLNRPGRLNALSIELMLELTDLVTRI
                     SEDDAVRVFVIRGAGRAFGPGTDLKEQHAKRSDRRFGRHIMKCMRGLQVAINSSPKVS
                     IAAVHGLALAGSLELMMTCDLAIATEDAHLSDQHANYGLPPVGSATQRLPRLVGTRKA
                     LELLLTGMSLTGTEAAALNLVSRAVPADRFEAATREFAESIANKSPLVTAMIKDLVYG
                     GIQADFNTGMMLEKWGGYASSAWEDVGEGIAAFNEKRRPNYRNR"
     gene            890..2545
                     /locus_tag="VN627_07630"
     CDS             890..2545
                     /locus_tag="VN627_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXD03162.1"
                     /translation="MTGSRLSPVSASGRRIITYDYVIIGAGSAGCAIAERLSADPAVS
                     VLLIEAGPSDNHPLIQMPKGFPFMAMNPRYAWNFMTEPQPHRNFGSEMWPRGKMLGGS
                     SSLNGMLYLRGHPGDYARWAEAAESDVWSWRQVARCFRELECHSLGESELRGGSGPLA
                     VTAGTVQSDLSEKMVAAGEQLGLRRLEDFNGPEQEGIGFYAVNIKDGLRFSAAKAHLR
                     RARGRRNLTIRSETLAERIVFEGTRAVGVVIRRGERSEVVRAGREVVVCTGTLLTPRL
                     LMLSGVGDAGHLRDHGVAVVADNAHVGRHLRDHVGLRLGYRLQGAQGANASLRWPRLA
                     GTIARYLLFRSGPLATTGEVGAFVRAHPKATRPDGQLMLSAMSQSAPRPGTYMGPVEK
                     LPGLSAHACVLDADSEGYIALASGDPADSPRIQPNGLSTAHDRDTLLNLVRIVRRLVA
                     QPALAPYIAHEAFPGPAINSDDEILDYANRTARNFNHAVGTCRLGSAATGAVDARLRV
                     HGVAGLRVADCSIMPSLPAGNTNGPAQMVGWRAAELIREDARG"
     gene            complement(2625..>3186)
                     /locus_tag="VN627_07635"
     CDS             complement(2625..>3186)
                     /locus_tag="VN627_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03163.1"
                     /translation="ESFRRELAGFTGKRAIFIHNAMYNAVGYVGEVDPAELAKALQAN
                     AVAPQLLGDMFLRAVGPGYESGLVQMSSAAARLPYAGMAAYSAVKAGIEIWVKAVLLE
                     LADRGRDTWVTAVRPGFVDTPGTRTTATRSPRDYPAATMVAQALEQGGPGVFTAEQSA
                     RLIWEALETRKHEPLLYFGTAVTTSS"
BASE COUNT          480 a         1022 c         1166 g          518 t
ORIGIN      
        1 gaccctagcg attgcggtag ttgggcctgc gcttctcgtt gaaggcggcg atgccttcgc
       61 cgacgtcttc ccaggcgctg gacgcatagc cgccccactt ctccagcatc atgccggtgt
      121 tgaaatcggc ctggatgccg ccgtagacca ggtccttgat catggctgtc acgagcgggc
      181 tcttgttcgc gatgctttcc gcaaattcgc gggtggcagc ctcgaaccgg tcggccggga
      241 cagcgcggct gacgagattg agcgcggcgg cctcggtgcc ggtcagcgac atgccggtga
      301 gcagcagttc gagcgccttg cgcgtgccga ccaggcgcgg caggcgctgg gtggccgagc
      361 cgaccggggg caggccgtag ttggcatgct ggtcgctgag gtgcgcgtcc tcggtggcga
      421 tcgccagatc gcaggtcatc atcagttcga ggctgccggc cagcgccagg ccgtggaccg
      481 ccgcgatcga gaccttgggc gagctgttga tggcgacctg caggcctctc atgcacttca
      541 tgatgtgacg tccgaagcgg cggtcgctcc gcttggcatg ctgttccttg agatcggttc
      601 cggggccgaa ggcccggccc gcgccccgga tgacgaagac acgcaccgca tcatcctcgg
      661 agatccgggt gacgagatcg gtcagctcca gcatcaactc gatactcagc gcgttgagcc
      721 gtccgggccg attgagggtg atccagacga taccgccgtc ttcctggtcg accagcaggg
      781 tgttgtaatc cacggcgggg ctctcctcat gcctgcgaga gccactgcat cccgcatgtg
      841 cgattgtttc gccggatgat tgcacgcggc cagagcggcg accagcacaa tgacgggttc
      901 caggctgagc cccgtctcag ccagcgggag acggatcatc acttacgatt acgtcatcat
      961 cggcgcgggt tcggcgggct gcgcgatcgc ggaacggcta tcggccgatc ccgccgtttc
     1021 ggtcctgctg atcgaggccg gtccttccga caatcacccg ctgatccaga tgcccaaggg
     1081 ctttcccttc atggcgatga acccgcgcta tgcgtggaac ttcatgaccg agccccagcc
     1141 ccaccgcaat ttcgggagcg agatgtggcc ccggggcaag atgctggggg gcagttcttc
     1201 cctcaacggg atgctctacc tgcgcggcca tccgggcgac tatgcccggt gggccgaggc
     1261 cgcggagagc gacgtgtggt cgtggcgcca ggtggcgcgg tgtttccgcg agctggaatg
     1321 ccacagcctg ggtgaaagcg agctgcgcgg aggcagcggg ccgctggcgg tgaccgccgg
     1381 cacggtccag tccgacctgt cggaaaagat ggtggcggcc ggcgagcagc ttggactgcg
     1441 gcggctggag gatttcaacg ggcccgagca ggaagggatc gggttctatg cggtgaacat
     1501 caaggatggc ctgcgcttca gcgccgccaa ggcgcacctg cgccgcgcgc gggggcggcg
     1561 caacctgacc atccgctcgg aaacgctcgc cgaacgcatc gtcttcgagg ggacccgcgc
     1621 ggtgggcgta gtgatccgcc ggggcgagcg gtccgaggtg gtgcgcgcgg ggcgcgaggt
     1681 ggtggtctgc accggcacct tgctgacccc gcggctgctg atgctgtcag gggtgggcga
     1741 tgcggggcac ctgcgcgacc acggagttgc ggtggtggcc gataatgccc atgtcggccg
     1801 gcacctgcgc gaccatgtcg gcctgcggct cggctaccgc ctgcagggtg cgcagggcgc
     1861 caatgccagc ctgcgctggc ccaggctggc ggggacgatc gcacggtatc tgctgttccg
     1921 gtccggtccg ctggcgacga ccggcgaggt gggcgccttc gtccgcgccc acccgaaggc
     1981 gacgcggccc gacgggcagc tgatgctctc ggccatgtcg cagagcgcac cgcgcccggg
     2041 aacatacatg gggccggtgg agaagctgcc cgggctttcg gcccatgcct gtgtgctcga
     2101 tgccgatagc gaggggtaca tcgcgctggc ctcgggcgat ccggctgaca gcccccgcat
     2161 ccagcccaat gggctgagca ccgcgcatga ccgggacacg ctgctgaacc ttgtcaggat
     2221 cgtgcgccgg ctggtcgccc agcccgcact cgcaccgtac atcgcacatg aggccttccc
     2281 cgggccggcg atcaactcgg atgacgagat tctggattat gcgaaccgca cggcgcgcaa
     2341 cttcaaccat gcggtcggca cctgccggct gggttcggcg gcgaccggcg ctgtcgacgc
     2401 acggctgcgg gtgcacggcg tggccgggct gcgggtggcc gattgctcga tcatgccgag
     2461 cctgccggcg ggcaatacca atggcccggc gcaaatggtc ggctggcgcg cggcggagct
     2521 gatccgggag gatgcgcggg gctgaccggc ggcgcgggcc ccgcagcgcc ggaccccctc
     2581 cccgccgggg aggggtgggg aagggggacg gcatcgggga gcggtcagct gctcgtcgtg
     2641 acggcggtgc cgaagtagag caggggttcg tgcttgcggg tttccagcgc ttcccagatc
     2701 agccgggccg actgttccgc cgtgaacacg ccgggaccgc cctgttcgag tgcctgggcg
     2761 accatcgtgg cggccggata gtcacgcggg gagcgggtgg cggtagtgcg ggtgccgggg
     2821 gtatcgacga agccggggcg caccgcggtg acccaggtgt cccggccgcg atcagccagt
     2881 tcgaggagta cggccttgac ccagatctcg atccccgcct tgaccgcgga gtaggccgcc
     2941 atgccggcat aggggagccg ggccgcggcg gacgacatct ggacgaggcc ggattcgtag
     3001 ccggggccga cggcccgcag gaacatgtca cccagcagct ggggagcgac tgcattggcc
     3061 tgaagcgcct tggccagttc ggccggatcg acctcgccga cgtagccaac ggcattgtac
     3121 atcgcgttgt gaatgaagat tgcgcgcttg ccggtgaagc ccgcgagttc gcgccggaag
     3181 ctttcg
//
LOCUS       DATNBV010000320         6684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4017238,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000320 DATNBV010000000
VERSION     DATNBV010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4017238"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VN627_07640"
     CDS             complement(<1..297)
                     /locus_tag="VN627_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03164.1"
                     /translation="MPRHLRAAAGTVLAAFLLGTCAPALAQASANPDGEARLRKLEAE
                     VRALQRQVFPGGDGKYFAPQVTAPTAPVAVPGTPASTPLSDLLTRMDAVEAQIAR"
     gene            complement(447..1241)
                     /locus_tag="VN627_07645"
     CDS             complement(447..1241)
                     /locus_tag="VN627_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXD03165.1"
                     /translation="MLDFVPLGPMFARQRGRSISDGTGMAEELRRDGVIEFPNVRLTA
                     GGRLLAAREAAGLTLVDMAGETKIPVRMLTLIEAGDYAALPAKAYATGFSRTYARALG
                     LDENEILAEVRRELGQVDHGETRLAPAFEPGDPARVPTARFAWAAAVAALAVLVAGLV
                     LWRSYYAPAVTLPPLTEPPPPRAVAAPARAPVVVVPLAPASGAPVAPELPTGAAPVAP
                     VVAAAPRHSQAKSGAAAPGAAPAPSPSANPVGAAPAVPAPASTAQF"
     gene            complement(1393..3666)
                     /gene="ptsP"
                     /locus_tag="VN627_07650"
     CDS             complement(1393..3666)
                     /gene="ptsP"
                     /locus_tag="VN627_07650"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HXD03166.1"
                     /translation="MPSAVAAARMILTRLHEVMASRMNAQAKLNTVVEVIGEALDSEV
                     CSIYLLREGMLELFATRGLAQEAVHVTRMAIGEGLVGTIGGNVETLNLAEATAHPDFS
                     YRPETGEDKFHSFAGVPIVRRERSVGVLCVQHVDPRRYEEVEIEALQTVAMVLSELIA
                     NADLVDEDEGAGLGPAHTGMELLRGLTLVKGLASGFAVFHQPRVTIEHTVAEDTEAER
                     QRVYLAFDKMRDQIERMAAQAEFGVGGEHEEVLQTYKMFAYDEGWSRRINEAIDSGLT
                     AEAAIERVQQRTRMRMRQIDDPLLAERMHDLEDLSNRLLRTVSGQLGTAASKGLRQDS
                     ILIARNLGPAELLEYDRRRLKGVILEEGSLTAHVVIVARAMGVPVLGRVRGMRGMVRE
                     GDLLLIDADSGTATVRPTPAIADAFEARFAKNRERQATYAAMREVEPRTLDGTRITVM
                     MNAGLRDDMAMLNLTGADGIGLYRTEFQFLVSATLPARERQTRLYREVLEAAGSRPVV
                     FRTVDIGGDKALPYLRHDDGDGEENPAMGWRALRVALEREGLLKAQARALLEAAAGRT
                     LNVMFPMVSEPWEFDAAKAVFDQQLDFLRKQKRMLPEAIRFGAMLEVPALAEVLDILA
                     PKISFLSIGTNDLTQFLFAADRANPKLAERYDWLSPSILRFLRRVLKGVEGYPVDVTV
                     CGEMGGRRLEAMALLGIGLRRLSITPVSVGPIKEMVRQIDLGELAEAMDGWLAVPPPS
                     LRLAMADWAAARGIECD"
     gene            complement(3798..3953)
                     /locus_tag="VN627_07655"
     CDS             complement(3798..3953)
                     /locus_tag="VN627_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXD03167.1"
                     /translation="MESSHVSALQMKHAGIERQLAEELSRPAPDQSMIQVLKKRKLRI
                     KEELSHH"
     gene            4259..4423
                     /locus_tag="VN627_07660"
     CDS             4259..4423
                     /locus_tag="VN627_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXD03168.1"
                     /translation="MLRIEHRDLDAAIDALTTAAPADQLQIARLKKRKLRLKDQIALI
                     EDYLIPDIIA"
     gene            complement(4552..5223)
                     /locus_tag="VN627_07665"
     CDS             complement(4552..5223)
                     /locus_tag="VN627_07665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HXD03169.1"
                     /translation="MAQISVTVITPTTGKASLERQVAAMDAQAGDVDFYHLLLWDDFR
                     SPGARPPESYNGPNRFSIVAPPGTGRNGAAPGSMLRAMGLMAARTDYVTFADDDVWWE
                     PDHLQALAGCVAGGLGWGSVLRRMWSPDGRYLGVDRFESVGDGPGRLVPYEMCDNNVM
                     VFRRELGVRAALLYRETTEYNDDRLMYGFLKENAGPRGGCDRATINHVCPDFLIEFFT
                     EFCTP"
     gene            5432..6340
                     /locus_tag="VN627_07670"
     CDS             5432..6340
                     /locus_tag="VN627_07670"
                     /inference="COORDINATES: protein motif:HMM:NF006140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXD03170.1"
                     /translation="MEYQYLTTERLDDGQIMRITLNRPAARNAQNRGLLVELDHAFLA
                     AEKDDEVRVVILAGNGPMFSAGHDLGSEESLTERLPGPDQHESFKGNGGTRKGGEQRM
                     LQEWHYYYQNTLRWRNLRKITIAQVHGKVIAAGLMLMWACDLIIAAEGTTFSDPVGAR
                     LGMCGMEYFGHPWEFGPRKAKELLLTTDSIDADEGYRLGMITKVYKGDELAEKTLEFA
                     RRIAQRPTMAALMIKESVNQAVDNMGFYNALQACFTIHQANHSHWAECHGTHEEPFRN
                     YPAALVEDGAEDWRKPNPTRPALKDN"
     gene            6411..>6684
                     /locus_tag="VN627_07675"
     CDS             6411..>6684
                     /locus_tag="VN627_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HXD03171.1"
                     /translation="MAETIIWISGATSGIGLALARTAPFPDTRIISFSRTPHPDYETV
                     HLDLTEPDTYAAVGESFRRELAGFTGKRAIFIHNAMYNAVGYVGEVD"
BASE COUNT         1144 a         2421 c         2111 g         1008 t
ORIGIN      
        1 ccgggcaatc tgcgcctcga ccgcatccat ccgagtgagc aggtcgctca gcggcgtgct
       61 ggccggagtc cccggcaccg ccaccggcgc ggtcggagcc gtcacctgcg gggcgaagta
      121 cttgccgtcg ccgccgggaa acacctggcg ctgcaaggcg cgaacctcgg cctcgagctt
      181 gcgcaggcgg gcctcgccgt cagggttggc cgaagcctgc gccagcgccg gggcgcaggt
      241 gccgagaagg aaagccgcca gcaccgtacc ggctgccgcg cgcaggtgcc gtggcatcgt
      301 cattatccca tggccgttca tcgtgaattc gttgctcccg tcgcttcatc ggccggcgcg
      361 atcccccgcg ccggcaaggc gtgccccgcc tcgtacccta aggccaaaaa ccttagccag
      421 accgccccgc tcctgtcgag acagcctcag aactgagcgg tggaagccgg cgccggaaca
      481 gccggggcgg ctccaaccgg gttcgcggag ggcgaaggtg cgggcgccgc gcccggcgcg
      541 gcggcgccgg atttcgcctg gctgtgcctt ggcgcagcgg ccacgaccgg agcgaccggc
      601 gccgcgcccg tcggcagctc gggagccacc ggcgccccgc tggccggcgc caggggcacc
      661 accacgaccg gcgcccgggc cggcgcggcc acggcgcgcg gcggaggcgg ctcggtcaag
      721 ggcggcaagg tcaccgcagg ggcatagtag gagcgccaca gcaccagccc ggccaccagc
      781 acggcgagcg cggccacggc ggcagcccag gcaaaccgcg ctgtcggcac ccgtgcggga
      841 tcgcctggct cgaaggctgg tgccagccgc gtctcgccgt ggtcaacctg ccccagttca
      901 cgccgcacct cggccaggat ttcgttttcg tcgagcccca gcgcccgggc ataggttcgc
      961 gagaagcccg tggcataggc cttggccggc agggcggcat aatcgccagc ctcgatcagg
     1021 gtcagcatgc ggacggggat cttggtttcc cccgccatgt ccaccagcgt cagcccggcg
     1081 gcctcgcgcg cggcgagcag ccgccctccg gcggtaagcc gaacgttcgg aaactcgatt
     1141 acgccatcgc gtcttagttc ttcagccatt ccggtcccgt cggatatgga gcgcccccgc
     1201 tggcgcgcga acatcgggcc aagcggaaca aagtcaagca ttcgctaaca tgggcgagtc
     1261 gcaaagcgac aggacggctc cggcagccga tcagccgaga acgcgccggg gaagcaaggc
     1321 cagtgccccc gctacccaca aagcgccatg cccggcccgc ttgacagcac cgcaactgcc
     1381 gggggatcgg tcctaatcgc actcgatgcc gcgggccgcg gcccagtcag ccatggccag
     1441 gcgcaggctc ggcggcggca ccgccagcca gccgtccatc gcctcggcca gttcgccaag
     1501 gtcgatctgg cgaaccatct ccttgatcgg gcccaccgat accggcgtga tcgacagccg
     1561 tcgcaggccg attccgagca gggccatggc ctccagccgc cgcccgccca tttcgccgca
     1621 gaccgtcacg tcgaccggat agccctccac ccccttgagc acccggcgca ggaaccgcag
     1681 gatcgagggg ctcagccagt cgtaccgctc ggccagcttg ggattggcgc ggtcggcggc
     1741 gaacaggaac tgcgtgagat cgttggtccc gatcgacagg aacgaaatct tgggcgcaag
     1801 gatatcgagc acctcggcca gcgccggcac ttccagcatg gctccgaaac ggatcgcctc
     1861 gggcagcatc cgcttctgct tgcgcaggaa atccagctgc tggtcgaaca ccgccttggc
     1921 cgcgtcgaac tcccacggct cggacaccat cgggaacatc acgttgagcg tccgcccggc
     1981 cgccgcttcc agcagggcgc gagcctgggc cttgaggagc ccctcgcgct cgagcgccac
     2041 gcgcagggcg cgccagccca tggccgggtt ttcctcgccg tcgccgtcgt cgtggcgcag
     2101 gtagggcagg gccttgtcgc cgccgatgtc gaccgtgcgg aacaccaccg gccggctccc
     2161 cgccgcttcg agcacctcgc gatagagccg cgtctgccgc tcgcgcgcgg ggagcgtcgc
     2221 ggaaacgagg aactggaact cggtccggta cagcccgatc ccatccgccc cggtgaggtt
     2281 gagcatggcc atgtcgtcgc gcagcccggc gttcatcatg acggtgatcc gcgtgccgtc
     2341 gagcgtgcgc ggctccacct cgcgcattgc ggcataggtc gcctgccgct cgcgattctt
     2401 ggcgaagcgg gcttcgaagg catcggcaat cgccggagtc ggccggaccg tggccgtgcc
     2461 ggaatcggcg tcgatcagca gcaggtcgcc ctcgcgcacc atgccgcgca tcccgcgcac
     2521 gcggcccagc accggcacgc ccatcgctcg cgccacgatc accacgtggg cggtcagcga
     2581 gccctcctcg aggatcacgc ccttgagccg ccgccggtca tattcgagca gttcggccgg
     2641 ccccagattt cgcgcgatca ggatcgaatc ctgcctgagg cccttgctcg ccgccgtccc
     2701 caactggccc gaaacggtcc gcagcagacg gttggacagg tcctccaggt cgtgcatccg
     2761 ctcggccagc agcgggtcgt cgatctggcg catgcgcatg cgggtgcgct gctgcacccg
     2821 ctcgatcgcg gcctccgccg tcagccccga atcgatcgcc tcgttgatcc gccggctcca
     2881 gccctcgtcg taggcgaaca tcttgtaggt ctggagcacc tcctcgtgct cgccgcccac
     2941 cccgaattcc gcctgggccg ccattcgctc gatctggtcg cgcatcttgt cgaaggccag
     3001 atagacccgc tggcgttcgg cctcggtatc ctcggccacc gtatgctcga tcgtcacgcg
     3061 cggctggtgg aacacggcga agccgctggc gagacccttc accagggtca gcccgcgcag
     3121 cagctccatg ccggtatgcg ccggccccag ccccgcgccc tcgtcctcgt ccacgaggtc
     3181 ggcattggca atcagctcgg agagcaccat cgccacggtc tgcagcgcct cgatctccac
     3241 ttcctcgtag cggcgcggat cgacatgctg gacgcacagc acgcccaccg agcgctcccg
     3301 ccggacgatc ggcacgccgg cgaaggagtg gaacttgtcc tcgcccgttt ccggccgata
     3361 ggagaagtcc gggtgggccg tcgcctcggc gaggttgagc gtctcgacat tgccgccgat
     3421 cgtgccgacc agcccctccc cgatggccat gcgggtgacg tgcacggctt cctgcgccag
     3481 tccgcgggtg gcgaacagct cgagcatgcc ctcgcgcagc aggtagatcg agcagacctc
     3541 gctatcgagc gcctcgccga tcacctcgac cacggtgttg agctttgcct gcgcgttcat
     3601 gcgcgacgcc atcacctcgt gaaggcgggt aaggatcatg cgggcggcgg caacggcgga
     3661 tggcatgaaa gccggttacc cgaaaccggg gcccttggaa acgggtggag aaggcagcgc
     3721 cggcccggca cctgccggaa tgtgtccgat ccggtagaaa agaccgttcc cggcggcgaa
     3781 aagccggcgc gggcccatca atggtgcgag agttcttcct tgatcctgag cttgcgcttc
     3841 ttgaggacct ggatcatcga ttggtctggc gcgggccggc tcagctcttc ggcgagctgc
     3901 cgttcaatac ctgcgtgctt catctgcaag gcgctgacgt gcgacgattc catgagcata
     3961 cctcctgagt tgctcctgct ccggtcgtat tcccgacctt cgcgcgcccg gctcgtcgca
     4021 gccgttggta acaccgactc aggtactgga cgaacccggt agcgcgattg tcaggaaagc
     4081 acattaagcc taggtaacga agcgaatacg agccgaaagc ggtggatttg gcccggcctg
     4141 cgccaagccg caggtcggcg gaaatgcccg cttcgtcgcc cggccgggcg actggctctg
     4201 gaatggatca ggtcaggtga acaccgtcag cgaagaagaa ctgcgcaagc gcttggaaat
     4261 gctcaggatc gagcatcgcg atctcgacgc cgcgatcgac gcgctgacca ccgccgcccc
     4321 ggccgaccag ctccagatcg cccgcctcaa gaagcgcaag ctcaggctga aggaccagat
     4381 cgccctgatc gaggactacc tgatccccga tatcatcgcg taattgtcgg gtacgggaag
     4441 cgcatcggcg tttcccatgc ggcgagtctc ttgttgggtg cgccctccac atccgtggcg
     4501 ggggagtggg ctggtgccgc aagggaaacc cggatgggtt tcccgcactc atcaaggcgt
     4561 acagaattcc gtaaagaact cgatcaggaa gtccgggcac acgtggttga tcgtggccct
     4621 gtcgcatccc ccgcgcggcc ccgcattctc cttgaggaaa ccatacatca gccggtcgtc
     4681 gttatactcg gtcgtctcgc gataaagcag cgccgcccgc acccccagtt cgcggcggaa
     4741 caccatcacg ttgttgtcgc acatctcata gggcaccagc cgccccggac cgtcgcccac
     4801 cgattcgaac cggtccacgc cgaggtagcg cccgtccggg ctccacatcc gccgcaggac
     4861 cgagccccag ccgaggccgc ccgccacgca gccggccagg gcctgcaggt ggtcgggctc
     4921 ccaccacacg tcgtcgtcgg caaaggtcac atagtcggtc cgggccgcca tcaggcccat
     4981 cgcccgcagc atcgatcccg gcgccgctcc gttgcgcccc gtgcccggcg gggcgacgat
     5041 gctgaagcgg ttgggcccgt tgtagctttc gggcggccgc gccccgggcg aacggaaatc
     5101 gtcccacagc agcaggtgat agaagtccac gtcgccggcc tgcgcatcca tggcggcgac
     5161 ctgccgctcg agcgatgcct tgccggtcgt cggcgtgatc accgttaccg aaatctgcgc
     5221 cactttcgcc ccctgcagcc aatctggccg atgccatatt gcccgctgcc aaaccgcaca
     5281 agcgccctgc cgcaccgcgc cgcacatagc ggatatccag cccccccatt gcgaaggacc
     5341 ttgcgcggcc cgcgccaaaa gtgctgacgt cttcgctctt gcccgtggcg cgatcgccca
     5401 ggcaacaacc ggatcacgaa ggatgccacc gatggaatac cagtacctga ccaccgaacg
     5461 gctcgatgat ggccagatca tgcggatcac gctcaatcgc ccggcggccc gcaatgccca
     5521 gaaccgcggc ctgctggtgg aactggacca tgccttcctc gctgccgaga aggacgatga
     5581 ggtccgcgtc gtcatcctcg ccggcaatgg cccgatgttc tccgccggcc acgatctcgg
     5641 ctccgaggaa tcgttgaccg agcgcctgcc cgggcccgac cagcacgaaa gcttcaaggg
     5701 taacggcggg acccgcaagg gcggcgagca gcgcatgctg caggagtggc actactacta
     5761 ccagaacacc ctgcgctggc ggaacctgcg caagatcacc atcgcccagg tccatggcaa
     5821 ggtcatcgcc gccggcctga tgctgatgtg ggcctgcgac ctgatcatcg ccgccgaagg
     5881 caccaccttc tccgatccgg tcggcgcgcg cctcggcatg tgcggcatgg aatacttcgg
     5941 ccacccgtgg gagttcggcc cccgcaaggc caaggaactg ctgctcacca ccgattcgat
     6001 cgatgccgac gaaggctatc gcctcggcat gatcaccaag gtctacaagg gcgacgagct
     6061 ggccgagaaa accctcgaat tcgcccgccg catcgcccag cgtcccacca tggccgcgct
     6121 gatgattaag gaatcggtca accaggcggt cgataacatg ggcttctaca acgccctcca
     6181 ggcctgcttc accatccacc aggccaatca ctcgcactgg gccgaatgcc acggcaccca
     6241 cgaagagccc tttcgcaatt atcccgctgc cctggtcgag gatggcgcgg aagactggcg
     6301 caagcccaac ccgacccgtc ccgcgctcaa ggacaactga tcgggatcgg cgggcgcggc
     6361 cctgccgcgc ccgcccggtc cgggcaagac cagtcaggga aaggatggcc atggcggaaa
     6421 cgatcatctg gatcagcggc gccaccagcg gcatcggcct ggccctcgca cggaccgcgc
     6481 cctttcccga cacgcgcatc atcagcttct cgcgcacccc gcatccggac tacgaaacgg
     6541 tccacctcga cctgaccgag cccgatacct atgcggcagt gggcgaaagc ttccggcgcg
     6601 aactcgcggg cttcaccggc aagcgcgcaa tcttcattca caacgccatg tacaacgccg
     6661 tcggctacgt cggcgaggtc gatc
//
LOCUS       DATNBV010000321         2488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2101831,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000321 DATNBV010000000
VERSION     DATNBV010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101831"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..389
                     /gene="grxD"
                     /locus_tag="VN627_07680"
     CDS             60..389
                     /gene="grxD"
                     /locus_tag="VN627_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HXD03172.1"
                     /translation="MSDTNARIAEIVNGNDVVLFMKGTPLFPQCGFSSRAIAILDHLG
                     VEYASVDVLQDMDIRQGIKSFSDWPTIPQLYVKGEFVGGSDIMMEMFEAGELHQLMTE
                     AAVKQAS"
     gene            668..1504
                     /locus_tag="VN627_07685"
     CDS             668..1504
                     /locus_tag="VN627_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXD03173.1"
                     /translation="MALPTMDLRGRLMHNAIMTAPHPTPVPVPAATLVVFRRAADGSP
                     PELLMVERSAGMRFAAGAAVFPGGRIDPADHELAELAVSNHLPRDEAAARIAAIRETL
                     EEAGLMVGVSEPVTAGAAAQARAALLTDGRLGPVLAQFGWTLDLDQLVPFARWCPNHH
                     RAFDTRFYLADLGAGVVETLVDGTENSRIFWSSARDTLTAAGRGELHIIFPTRRNLER
                     LALHDDFAAAAAQARAHPVSTITPYGEERNGEPWWVIPEGQGYPVTGEPLATALHGLK
                     RT"
     gene            complement(1505..2299)
                     /locus_tag="VN627_07690"
     CDS             complement(1505..2299)
                     /locus_tag="VN627_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD03174.1"
                     /translation="MSSLFDLTGKTALITGSTKGIGREIAEAFVDHGARIAVCSRKLA
                     DAQAAAAEINARVGRDAAFGLSADLMDRASLIAAHDAAVARMGRIDVLVCNAAQTPED
                     YCSMLDYPADGYSKLMEGNIVNNAALMSHAARPMKERKDGVILATSSGGGVHPGYSTV
                     PYGVSKAGLNFLVAGLAAELAPFNVRVNAIAPGLTRSWSMEQTMQHNPAAMDVFVSRV
                     PMRRVIEGREIAAGMVFLASEGGKAITGETIVMTAGTAGNGAPPDA"
BASE COUNT          440 a          830 c          771 g          447 t
ORIGIN      
        1 gaccaccgcg cttcccacct gatcgcctga cgaaacatca atcaaggttg ccgaacccca
       61 tgtccgatac aaatgcccgc atcgccgaga tcgtgaacgg caacgacgtc gtcctattca
      121 tgaaaggcac gccgctgttt ccgcagtgcg gcttctccag ccgcgccatc gccatcctcg
      181 accatctcgg ggtcgaatat gccagcgtcg acgtgctgca ggacatggac atccgccagg
      241 gcatcaagtc gttttcggat tggccgacga tcccccagct ctacgtgaag ggcgaattcg
      301 tgggcggcag cgacatcatg atggaaatgt tcgaagccgg cgaactccac cagctgatga
      361 ccgaggctgc cgtcaagcag gcttcctgaa ctcagatccg tttcctgaaa ccagaaaggc
      421 ccgcgaacct gggtggttcg cgggcctttt ttggcatgcc gggtgggacc agcgtgccca
      481 gggggatatt tgctcgggaa ggcggggccg cggagaccag gatgcagccc cgcctcttga
      541 gactgcttgg ggtacacaag cattagcacg agccgtgcca aattcaggaa accgcggttt
      601 cctgagggat tgaacccctg cggcatccct gaatcacggg cgtgtgtaaa ttgcttcgac
      661 agtttgcatg gccctgccca ccatggattt gcgcggccgc ctgatgcaca atgccatcat
      721 gaccgccccg catccgacgc ccgttcccgt ccccgccgcc actctcgtgg tgttccgtcg
      781 cgccgctgat ggcagtccgc ccgagctgct gatggtcgaa cggtcggccg gaatgcgctt
      841 tgctgcaggg gcggccgtat ttcctggcgg gcggatcgat ccggccgacc atgaactggc
      901 ggagctggcc gtgtccaacc atctgcctcg tgacgaggcg gcggcccgta ttgccgccat
      961 tcgggaaacg ctggaagaag cgggtctgat ggtcggcgta agcgagccag tcactgccgg
     1021 ggccgcagca caggcacggg cggcgcttct cactgatggg cggctcggcc cggtgctggc
     1081 gcagttcggg tggacgctcg acctggatca actggtcccc ttcgcgcgct ggtgccccaa
     1141 tcaccaccgc gccttcgata cccggttcta cctcgcggat cttggcgccg gcgtggtcga
     1201 aacgcttgtc gatggaaccg aaaacagccg gatcttctgg tcatccgccc gtgacacgct
     1261 caccgcagcg gggcgcggcg agctccacat catctttccc acccgccgca atctcgagcg
     1321 actggccttg catgacgatt tcgccgcggc cgcagcccag gcgcgggccc atccggtaag
     1381 cacgatcacg ccttacgggg aagagcgcaa cggcgagccg tggtgggtga tccccgaagg
     1441 acagggctac ccggtcaccg gcgagccgct ggccaccgcg ttgcacgggc tgaagcgcac
     1501 ctgatcaggc gtcgggcggc gcaccgttcc ccgcggttcc agcggtcatc acgatggtct
     1561 cgccggtaat cgccttgccg ccctcggaag ccaggaagac catccccgcg gcaatctcgc
     1621 gtccttcgat gacccgacgc atcggcacgc gagagacgaa tacgtccatc gcggcgggat
     1681 tgtgttgcat cgtctgctcc atcgaccacg agcgggtcag gccgggcgcg atcgcgttca
     1741 cccgcacgtt gaacggggcc agctcagctg cgagaccggc aaccaggaag ttgaggcccg
     1801 ccttcgagac gccgtagggc acggtcgaat acccaggatg caccccgcct cccgacgagg
     1861 tggcgaggat caccccgtcc ttgcgctcct tcatcgggcg agcggcatgg ctcatcaacg
     1921 ccgcattgtt gacgatattc ccctccatca gcttcgaata gccgtcggcg ggatagtcaa
     1981 gcatcgagca gtaatcctcc ggcgtctgcg ccgcgttgca gaccagcacg tcgatccggc
     2041 ccatccgcgc caccgcggca tcgtgcgcgg caatcaggct ggcgcggtcc atcaggtcgg
     2101 ccgagaggcc aaaggcggcg tcccgaccaa cccgagcatt gatctcggcg gcggcagctt
     2161 gcgcatcggc cagcttgcgc gagcagaccg cgatccgggc gccatgatcg acgaaggcct
     2221 cggcgatctc gcgaccgatt cccttggtcg accccgtgat cagcgcggtt tttccggtca
     2281 ggtcaaagag cgagctcacc attcattctc tcctcggtct gccgcctttt gccgggggct
     2341 taattttcct cgatccggcg ccgatggagc aggccctgtc gcgatcattc agcgacacgg
     2401 ctagtcccga aaacctgagc gggcatgctc gttggtatgt tcaggagagc tggtcggcaa
     2461 gccattgcgc gtactgcaca tttatgag
//
LOCUS       DATNBV010000322         4957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1469882,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000322 DATNBV010000000
VERSION     DATNBV010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469882"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VN627_07695"
     CDS             complement(<1..289)
                     /locus_tag="VN627_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXD03175.1"
                     /translation="MATTAATAAPVFDRETLLAAARDRTGLSDFGDTWFFEPMDRYIA
                     AANAEGKLTPDGFAGQTESIVKGLASRLRMVEDIKRHPGIMDETVEVAAIIL"
     gene            complement(455..2041)
                     /locus_tag="VN627_07700"
     CDS             complement(455..2041)
                     /locus_tag="VN627_07700"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HXD03176.1"
                     /translation="MEKLEFDYVIIGGGVAGCILANRLSADPRTRVLLLEAGGNDRSP
                     LIAAPGGLLPIMMSGAHAWPYMSAPQRHLDDRVLYLPRGKVLGGGSSINGMTYDRGFH
                     SDYDRWAQAGNRDWSFADVLPYFRKLENFGPAHNAWHGKDGPIQVTRADQRHPFARAF
                     IAAGRQAGYPVTDDLNGETRDGFGAVDLTVGRGRRSSASSAYLRPVLKRPNLTVLTGA
                     HSRRVVFDGTRATGVEFRKDGADSLAAASREVILSAGAINTPQLLMLSGVGPAAHLAE
                     HGIALVRDLPGVGQGLQDHLAAHVKYRSTKPWSMLRFLNPLRGALAMVQYLALRTGPL
                     ADPGMSVACAVRSDPALDEPDIKMLLVSALMAHNGREMVPMHGFYAHINVARPESRGS
                     VTLASADPDVPPVIDQNYNATENDRRVLREGVRIARRIFAQPAFDEMRGAELAPGKDV
                     QSDAEIDAYVRAHAEADYHSTSTARMGSDPLAVVDDRLRVHGMQGLRVVDASVMPHLP
                     GGNTAIPVAMIAEKAADMIL"
     gene            2228..2455
                     /locus_tag="VN627_07705"
     CDS             2228..2455
                     /locus_tag="VN627_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HXD03177.1"
                     /translation="MRVIANHQLCQGHARCEDLCPEVFATDAVEGKVVIVMAQVPPAL
                     EDKVRLAVRNCPEGALRVTRQEGAASKGELQ"
     gene            2452..3693
                     /locus_tag="VN627_07710"
     CDS             2452..3693
                     /locus_tag="VN627_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXD03178.1"
                     /translation="MTLRFQPPPPDHVPAERMVDFDMFHIPPGVTDPAEIWHDLVRRG
                     VPDIFYTPHNGGHWVFLRYEDIVEGYRDYSIFSTYQTPVPPIEPFPVVQPQGVDPPEH
                     NVFRRLLAPMFTPIAVRAMTGELERRASVLIEQFAARGECDFIADFAERYPTGTFLYL
                     FGLPEERLDEFLALANTFFRSTDPAERARNITEIYAVLDQLFRDKQRNPGDDIASRIV
                     AARDEEGQPHAWEDILNCGFLLFVAGLDTVTNTMAYVWRYLATTPAAQRHFRERLDDP
                     DAFLRAIEELMRINAVSNLYRRVTHDCTYKGVQLRRNDRVILPNTVANRDPRVFKDPQ
                     VIDLEREVNVHVTFGVGPHRCIGSQLAKREIMVSLQQWLRRIPEFTLAEHQDEGSVFG
                     GSVMGFTALRLHWEAAAKAAA"
     gene            complement(3743..4435)
                     /locus_tag="VN627_07715"
     CDS             complement(3743..4435)
                     /locus_tag="VN627_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03179.1"
                     /translation="MATSAPDIETSAISDGGLSMGHLALHYGTPGDGPAASRLLQIAG
                     LKETQVLPLPQGNFYRFVVNGGHFSRGDGIVYLSCLPEPQQKLIAAIHEALRVGTNDE
                     HEAVKGYRAMMAQDFEASFHFGFLVESLEELEAMVLKLQDLSENDPTFKGRLNIGMNR
                     ARRGDAEVDARLDASPVFGKATRYAYGAAGVQVFVETDLVRAGQLGENMYFEFDYVFP
                     DRTSHILSVVEL"
     gene            4665..>4957
                     /locus_tag="VN627_07720"
     CDS             4665..>4957
                     /locus_tag="VN627_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXD03180.1"
                     /translation="MQRLALDHMTAIDTTPSQLAQVARSVGCDGICLFMEPMDVLPLM
                     PPFDIYGDRSARAATRTVMNDLGVALDLAYPFTLAGRTEIAGFATAMECAADLG"
BASE COUNT          844 a         1589 c         1655 g          869 t
ORIGIN      
        1 cgaggatgat ggcggccacc tccacggtct cgtccatgat cccggggtgg cgcttgatgt
       61 cctccaccat gcgcagccgg ctggccagcc ccttgacgat cgattcggtc tgcccggcga
      121 agccgtccgg ggtcagcttg ccttcggcat tggcggcggc gatgtaccgg tccatcggct
      181 cgaagaacca ggtgtcgccg aaatcggaga gacccgtgcg gtcccgcgca gcagccagca
      241 gggtctcccg gtcgaagacc ggcgcagcgg tggcggcggt tgttgccatg gcagtgatgt
      301 ctctcccgag atcgcaaatg caaacatgcg taacacaagg cgcctctgtg ttcgcaattg
      361 aacgcatttt gtcacttgca aaggcatttt gcaaatacct ttgtccgaga aaacgatgtt
      421 caggcccctg cgctcgagag cggcatcagg tgccttacag gatcatgtcc gcagcctttt
      481 cggcgatcat tgccacgggg atcgcggtgt tgccgccggg caggtgaggc atgaccgatg
      541 catcgaccac gcgcaggccc tgcatgccat gtacacgcag ccgatcatcc acgacggcca
      601 gcggatcaga tcccatccgc gccgtgctgg ttgagtggta atcggcttcc gcatgggcgc
      661 ggacataagc gtcgatctcc gcgtcgcttt gcacatcctt gccgggcgcc agttccgctc
      721 cccgcatctc gtcgaacgcg ggctgtgcga aaatgcggcg ggcgatgcgc acgccttcgc
      781 gcaggacccg gcggtcgttc tcggtggcgt tgtagttctg gtcgatcacc ggaggcacgt
      841 ctggatcggc gctggcaagg gtgacgctgc cccgcgattc cgggcgcgcg acgttgatgt
      901 gggcgtagaa gccatgcatc ggcaccatct cgcgcccgtt gtgcgccatc agggcggaga
      961 cgaggagcat cttgatgtcg ggctcgtcca atgcgggatc ggagcggacc gcacaggcca
     1021 ccgacatgcc cgggtccgcc agtgggcccg tgcgcagggc aaggtactgg accatcgcca
     1081 gcgcgccgcg cagcggattg aggaagcgca gcatcgacca tggtttggtc gagcgatact
     1141 tcacatgggc ggcaagatgg tcctgcaggc cctggccgac gccgggaaga tcgcggacca
     1201 aggcgatgcc gtgttcggcc agatgggcag cggggcctac gcccgagagc atgagtagct
     1261 ggggcgtgtt gatggcgccc gccgagagga tcacctctcg tgacgcggcc gccaggctgt
     1321 ccgcaccgtc cttgcggaat tcgacgccgg tggcgcgggt gccgtcgaag actacgcgcc
     1381 ggctgtgcgc cccggtcagc acggtgaggt tggggcgctt cagcaccggg cgcagatagg
     1441 cggaggaggc actggaccgc cggccgcggc cgacggtgag atctaccgcc ccgaacccgt
     1501 cacgcgtctc tccgttgagg tcgtcggtga cggggtagcc ggcctgccgc cccgccgcga
     1561 tgaaggcccg ggcaaaggga tggcgctgat cggccctggt cacctggatc ggaccatcct
     1621 tgccgtgcca cgcattgtgg gccgggccga agttttcgag cttgcggaaa taggggagca
     1681 cgtcggcaaa cgaccagtcg cgattgccgg cctgggccca gcggtcgtaa tcagaatgga
     1741 agccgcgatc ataggtcatg ccgttgatcg aggatccgcc gccgagcacc tttccccggg
     1801 gcaggtagag cacgcggtcg tcgaggtgcc gctgcggggc ggacatgtag ggccaggcat
     1861 gggcgcccga catcatgatc ggcagcaggc cgccgggcgc ggcgatcagc gggctccggt
     1921 cgttcccgcc cgcctccagc agcagcacgc gcgtgcgcgg gtcggccgaa agccggttcg
     1981 cgaggatgca cccggccacc ccgccgccga tgatcacgta atcgaattcg agcttttcca
     2041 tgctctccta catccttccc gacccaagac cccgcatcgt ggcggcccca tgtcccggct
     2101 gcaacgtgcg cgggcggcgg ggcagctttg atggcattat ctggctgaaa tggcgcaacg
     2161 ggtttgcccg aagcgcttgg gattgccgac ggaaccgcct tagctgatcg ggagaggaag
     2221 ctgaacgatg cgagtcatag ccaaccatca gctctgccag ggccatgccc ggtgcgagga
     2281 cctgtgcccc gaagtgttcg ccaccgatgc cgtggaaggc aaggtggtga tcgtaatggc
     2341 gcaggttccg cctgcgctgg aagacaaggt ccggctcgcg gtccgcaact gtcccgaagg
     2401 ggccttgcgc gtgacccggc aagagggtgc cgcgagcaag ggggaactgc agtgaccctc
     2461 cggttccagc cgcccccgcc cgatcacgtg ccggcagagc gcatggtcga cttcgacatg
     2521 ttccatatcc ccccgggcgt gaccgatccg gcggaaatct ggcacgacct ggtgcgccgc
     2581 ggcgtgccgg acattttcta caccccccac aacggcggac actgggtgtt cctgcgctac
     2641 gaggacatcg tcgaaggcta tcgggattac tcgatcttct cgacctacca gaccccggtg
     2701 ccgccgatcg agccgttccc cgtggtgcag cctcagggcg tggatccgcc ggagcacaat
     2761 gtgttccgcc ggctgctcgc gccgatgttc acgccgatcg ctgtgcgcgc gatgaccggg
     2821 gaactggagc ggcgcgcttc ggtcctgatc gaacagtttg ccgcaagggg cgagtgcgac
     2881 ttcatcgccg actttgccga gcgctatccg accggcacgt tcctgtacct gttcggcttg
     2941 ccggaagagc ggctggacga gttcctcgcc ctggccaaca cgttcttccg cagcacggac
     3001 ccggcagaac gggcgcgcaa tatcaccgaa atctatgccg ttctcgacca gctgttccgg
     3061 gacaagcagc gcaatccggg cgacgacatc gcctcgcgga tcgtggcggc ccgggacgag
     3121 gagggccagc cccacgcctg ggaggatatc ctcaattgcg ggttcctgct gttcgtggcc
     3181 gggctcgaca ccgtcaccaa tacaatggcc tacgtctggc gctacctggc gacaaccccc
     3241 gcggcgcagc gccattttcg tgaacggctg gacgatccgg acgcattctt gcgcgccatc
     3301 gaggagctga tgcggatcaa cgccgtgtcc aacctgtacc gccgggtgac gcatgactgc
     3361 acctacaagg gggtgcagct gcgccgcaat gaccgggtga tcttgcccaa cacggtcgcc
     3421 aatcgcgatc cccgggtgtt caaggacccc caggtgatcg acctcgagcg cgaggtgaac
     3481 gtccacgtta ccttcggcgt ggggccgcac cgctgcatcg gctcccagct cgccaagcgg
     3541 gagatcatgg tttcgctgca gcagtggctg cgccgtatcc ccgagttcac gctcgccgag
     3601 caccaggacg agggttcggt gttcggtggt tcggtgatgg gcttcaccgc cctgcggctg
     3661 cactgggaag cggcagcgaa ggcggcggcc tgaaccggag cctggccctg cgccgtcggc
     3721 cggcgcaggg ccgctacagc tgtcagagtt cgacgaccga gaggatgtgg ctcgtccggt
     3781 cggggaaaac gtagtcgaac tcgaagtaca tgttctcgcc cagctggccg gcgcggacca
     3841 ggtcggtttc gacgaagacc tggaccccgg cggcgccgta ggcatagcgg gtggccttgc
     3901 cgaacacggg cgaggcatcc agccgcgcgt ccacttccgc atcgccgcgc cgggcgcggt
     3961 tcatgccgat gttcaggcgg cccttgaatg tcgggtcgtt ctccgaaagg tcctggagct
     4021 tcaggaccat cgcttccagc tcctcgagcg actcgaccag gaagccgaag tgaaagctcg
     4081 cttcgaaatc ctgggccatc atggcgcggt agcccttcac cgcctcatgc tcgtcattgg
     4141 ttccgacgcg cagggcttcg tggatcgccg cgatcagttt ctgctggggt tcaggcaggc
     4201 acgagagata gacaatgccg tccccgcgcg agaaatggcc gccgttgacc acgaagcgat
     4261 agaaattgcc ctggggaagc ggcaggacct gcgtttcctt cagcccggcg atctggagca
     4321 ggcgcgatgc ggcggggccg tcgcccggcg tgccgtaatg cagggccagg tggcccatcg
     4381 acaggcctcc gtcggaaata gcagaggttt cgatgtccgg tgcgctcgtt gccatgtcca
     4441 gtatcctctc aagccccttg ccgattgatc cagcggcgag gcattctgcg ccggccatac
     4501 cagccactgc ggcatgtcgc aataaggtga gcgttcacta actaacatgt gcgtactgct
     4561 caggtggatt gcctccgtat cgcgatgatc gatcggggaa tgctcgtccg tttgcgtata
     4621 tgaaaacagt ggcaggccaa tcgcggaatg attacgcacg aaccatgcaa cgcctcgcgc
     4681 tcgaccatat gaccgccatc gacacgacgc cgtctcagct tgcgcaggta gcccggagcg
     4741 tcggctgcga cgggatctgc ctgttcatgg agccgatgga cgtgctgccg ctgatgccgc
     4801 cgttcgacat ctatggcgac cggtcggccc gggcggcaac ccgcacggtg atgaacgatc
     4861 tgggggtggc gctggacctt gcctatccgt ttacgctggc tggtcgcacc gagatcgccg
     4921 gatttgccac ggccatggaa tgcgctgcgg atctggg
//
LOCUS       DATNBV010000323         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4660091,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000323 DATNBV010000000
VERSION     DATNBV010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4660091"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..876)
                     /locus_tag="VN627_07725"
     CDS             complement(313..876)
                     /locus_tag="VN627_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="opacity protein"
                     /protein_id="HXD03181.1"
                     /translation="MKICFAAAALALALSGPAFAQDNPPPTAPDGSKAFGIEPYVGVL
                     GGYDNYSRRSKFGVNGGRDNMDGELVEGVAGVNVPLGAFFVGAEGTVSKGFKDIDWEY
                     GVRGRAGLRAGTSGLIYGSAGYMWVNGNHGFPDRKDWIYGLGVEVGPKDIGLGGITDS
                     AGPRLRLQVDTFQFHSIRPMAGVMFHF"
     gene            1175..1618
                     /locus_tag="VN627_07730"
     CDS             1175..1618
                     /locus_tag="VN627_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXD03182.1"
                     /translation="MPNSIRLHRVLAAKPEKVYRAFVEADAIASWLPPFGFLCTVHEL
                     DARIGGKHRMSFRNFTTGHSHSFGGEYLELVPGQRLVYTDRFDDPNLPGEMTVTVALK
                     AVSCGTEMSIEQAGVPDMIPPEACYLGWQESLRKLAKLVEPEINQ"
     gene            complement(1882..2157)
                     /locus_tag="VN627_07735"
     CDS             complement(1882..2157)
                     /locus_tag="VN627_07735"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXD03183.1"
                     /translation="MSDRIGCVRCDDGFPHVASPPGHPIDATIDWSEAMNAFMYRLSV
                     IHRRIDEEIRRELKRRVPDSIRLLRLKKLRLAVKDRLAGHVRKPRTA"
BASE COUNT          421 a          694 c          698 g          399 t
ORIGIN      
        1 ctcggaaagg atcgtgccgt tgttcacgcc gctcggggcc gcgcaggtgc atttgcaacc
       61 ggtcgtcgtt ccgccctgtt tgggtttggc gagcgccgat gccggcgcgc aggcgacgat
      121 ggcaatcgcc ccgatcaatg cgaatctgtt gaacatgtcc tgctcctgag gtcgttggtt
      181 cgttgcagac ccgtatttta tttggttcgc gcaacgcgga ggcggcttga ttgttccttt
      241 catcgcccga tgatcgtggc cagcgatacc tggtagaggc gcccggatcg tgaatccggg
      301 cgcctctcga cctcagaagt ggaacatcac gccagccatt ggccggatgc tgtggaactg
      361 gaaggtatcc acctgcaggc gaaggcgcgg gccggcgctg tcagtgatgc cgccaagccc
      421 gatgtccttg ggcccgacct cgacgccgag cccgtagatc cagtccttcc ggtcgggaaa
      481 gccgtgattg ccgttcaccc acatatagcc ggccgagccg tagatcagcc cgctggtgcc
      541 cgcgcgaagg ccggcacgcc cgcgcacgcc atattcccaa tcgatatcct tgaagccttt
      601 cgagacggtg ccttcggcgc cgacgaagaa tgcgcccagc ggaacattga cgccggcgac
      661 accttccacc agctcgccgt ccatattgtc gcgcccgccg ttgacgccga atttcgagcg
      721 gcgactataa ttgtcgtaac cgcccaggac gccgacatag ggctcgatcc cgaaggcctt
      781 gctgccatcg ggcgccgtcg ggggcggatt gtcctgcgca aaggcagggc cggaaagggc
      841 gagggccagc gcggcggctg caaaacagat cttcataaaa cacactcctt attcgccatc
      901 cgcgccgcaa tgcagcgaca gcgccgatgg agcggcccta acggggtgcg ctcggcgagg
      961 tttctcagct cgtcgcttga aataatggtg ttgcaaatca acaacatgca atgtgacgag
     1021 tgactgcata gatggggtga acggtgcaac cctatggccg cacatgcttt cgcgcgtgca
     1081 ccaacggggc ggcgatcacg aggcttgatg ccgcgcgttc caccattcat gctggcgctg
     1141 accgtagctt gcattgaacg agaggaaact ggccatgcca aacagcatcc gcctgcaccg
     1201 cgtgctcgcg gcgaagcccg agaaggtcta tcgcgccttt gttgaagccg atgcgatcgc
     1261 aagctggctt ccgccgttcg gctttctctg tacggtccat gagctcgatg ccaggatcgg
     1321 cggcaagcac cgcatgtcgt tccgcaactt caccaccggg cattcgcaca gcttcggcgg
     1381 cgaatatctc gagctcgtgc cgggccagcg gctggtctac accgatcgct tcgacgatcc
     1441 caatctgccc ggcgagatga cggtcacggt cgccctcaag gccgtgtcct gcggcaccga
     1501 gatgagcatc gagcaggccg gcgtaccgga catgatcccg cccgaggcct gttatctggg
     1561 ctggcaggaa tcgttgcgga agctggcgaa gctcgtcgag cccgagatca accagtgagg
     1621 cggccgccct tcgcgggcgg gccgaccgat aaaatccccc tcccttccag ggaggggatc
     1681 gaggggtggg tggggactga cggggctcga tgcccccgaa gtaccgattg cgaggtagtt
     1741 caatgagtcc cgtggcccgc agacggcggg cacccacccc tacgcccagt tggcgctgcg
     1801 gccctgtctg ggagaccggg ccagtcgcca gctcttgaaa gggaggggga agaaaatgaa
     1861 gcgaactttg attccgccgc gctaggcggt gcggggcttc ctcacatgcc cggcaaggcg
     1921 atccttcacc gcaagccgca gcttcttgag ccgcagcagc cggatggaat cgggaacccg
     1981 ccgcttcaat tcgcggcgga tctcttcatc gatccggcgg tggatgacag acaaacggta
     2041 cataaaggca ttcatggcct cactccaatc gatggttgca tcgatcgggt gacccggagg
     2101 actggcgaca tgggggaaac cgtcgtcgca ccggacacac ccgatgcggt ccgacatcac
     2161 gatccaagga aagatcgcca gaggggcccg gcccgcaggt ttaatctagg gc
//
LOCUS       DATNBV010000324         8183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1470421,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000324 DATNBV010000000
VERSION     DATNBV010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470421"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VN627_07740"
     CDS             <1..360
                     /locus_tag="VN627_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HXD03184.1"
                     /translation="RIDPEGQNFSQLAKTINSLRESGRNTEELDGKWWHRFSGALAAM
                     LMPLLGATAAFGLARSGALLIRSVIGMALGFAYFVIDNAALAIGNFGGYPPLVAAWAP
                     FLLFFCIGEAVLIRTEE"
     gene            complement(694..1809)
                     /locus_tag="VN627_07745"
     CDS             complement(694..1809)
                     /locus_tag="VN627_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXD03185.1"
                     /translation="MNSPAFPASKTAAPTAGKPASIGADDMAMLRAAAELTRDINTAR
                     AGIYWPDMLASALLGYGALAGAILLTGWAALACALLAALALYRALLFIHELTHIHRDA
                     LPGFRFAWNVLVGIPLLTPSFMYENVHTLHHARTRYGTADDPEYLPLALMKPWSLPGF
                     ILVALALPFALLFRFAVLVPLGALIPPLRKLVWQRASALSINPAFRRRAPEGAFARMV
                     LWQETGGALWALTLVGLSVRYGWRPLVTGLCVVSVVALLNQLRTLVAHLWQNEGEQMT
                     VTAQYLDSVNVPPPGIWAELWAPVGLRYHATHHLLPSLPYHSLAEAHRRLSDHAGAQA
                     TYAGATYPGLWMLIGRIAKSTMVRREARTIVWKPGEA"
     gene            1962..3122
                     /locus_tag="VN627_07750"
     CDS             1962..3122
                     /locus_tag="VN627_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HXD03186.1"
                     /translation="MAAPEIVVSPVTTKAERGTFVDLAYRLNADDPNWVPPLRMEAME
                     LVTPGKNPFFEHATVQYFLARRGAEVVGRISAHIDHLAVAMSPEQGMGPGVGNWGMLE
                     ATDEAVAHVLIAAAEDWLRGQGMTRVLAPLSMSIWEEPGQLVFGFDHPPTVMMGHQGE
                     RYQRWIESAGGYEPAKQLHTYELDITKEFPPLIQRIVASGEKNARIRIRNTNKKKFEE
                     EARLILDILNDAWSDNWGFVPFTETEIKYAGKKFKPIVHEDLIMVAEYDGEPVAFMMT
                     VPDLNEAILPMGGKLFPFNWIKLLGWLRKPRPRTMRVPLMGVRKKLQSSRMASQLAFM
                     MIEYIRRNAVSRYGSTRGEIGWILEDNQGMVAIAEAINSHVNKTYVIYGKAL"
     gene            3184..3864
                     /locus_tag="VN627_07755"
     CDS             3184..3864
                     /locus_tag="VN627_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HXD03187.1"
                     /translation="MKHSRHPLRLYLGQRIAPPRFLAFLVLLPLATWGQHALVGSDWT
                     AALALGFDFAAALFLVSLLPLILRHEADAIRCRADANDANRVVVLVISSMLTIVVMAA
                     IAGELAGARSGQLASVIVLVATLVLIWLFANSVYALHYAHEFYTPEAGDQGPRDRGGL
                     DFPGTPEPGYRDFAYFAFTLGMTFQTSDVAITASRIRAVAVLHSFAAFVFNIGVIAFT
                     INVLGGAS"
     gene            3987..4526
                     /gene="ppa"
                     /locus_tag="VN627_07760"
     CDS             3987..4526
                     /gene="ppa"
                     /locus_tag="VN627_07760"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HXD03188.1"
                     /translation="MRIDMIPIGENPPESLNVIIEVPVGGEPVKYEFDKASGALFVDR
                     ILHTPMRYPANYGFVPHTLSPDGDPLDALVIARSPFVPGSVVRARPIAVLNLEDEHGG
                     DEKLVCVPDDKTFPYYSDVAEKGDLPEIVLQQIEHFFTHYKDLEKEKWVRVGKWGGAD
                     DAKRITLEAIERAKQAKAA"
     gene            4667..4972
                     /locus_tag="VN627_07765"
     CDS             4667..4972
                     /locus_tag="VN627_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXD03189.1"
                     /translation="MRQRRPCTYILASQKGGTLYIGVTSDLPARIWQHRHGTTGGFAS
                     RYRVYRLVHAEFHEDMPSAIAREKQLKNWHRQWKINLIEAVNPHWLDLARKWGIAEA"
     gene            complement(5049..5690)
                     /locus_tag="VN627_07770"
     CDS             complement(5049..5690)
                     /locus_tag="VN627_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXD03190.1"
                     /translation="MTSSELAREALPQSIKRRVKRMLGPWGVFVEGFLRHPVMVGSII
                     PSSRFTVRKMLGPVDWDNCKLFVEYGPGIGTFCRPVLDRLRRDGQLIVIDTNPLYIDY
                     LRRAITDSRFTAVLGSAAEVEEIVRAHGHDHADYVLSGLPFSTLPEGVGPAIAAATHR
                     VLRPGGAFLVYQFTAKARDFMARHFQRIDSGFEWLNVMPCFLFWGWKQEAAEG"
     gene            complement(5837..6379)
                     /locus_tag="VN627_07775"
     CDS             complement(5837..6379)
                     /locus_tag="VN627_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXD03191.1"
                     /translation="MASLVLNDQPVEIAADPAMPLLWALRDLANLTGTKYACGVGECG
                     ACTVLVDGQPLPACTLTMAEAEGRSVTTIEGLARRAPGVPRHPVLQALIDEQAIQCGF
                     CTPGIAMALVGLLARNAHPADEDIAAAITNLCRCGVYPRIVPAVRRVAGALTPAPAGT
                     TPSPADSPAAEPTEPAASGE"
     gene            complement(6534..6983)
                     /locus_tag="VN627_07780"
     CDS             complement(6534..6983)
                     /locus_tag="VN627_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HXD03192.1"
                     /translation="MAETGDVTTLNTLITTLIDSIEGYETSAGDIRDPALAQRFTARA
                     QERRRAVTDLQESVRRLGGNPADDGSIAGGAHRMFVNLKQAVIGSDDKAIVNEVERGE
                     DYLKGKFEAALKDEGLSGEARSAVQQAWESVRSGHDEMSRLKHSMGG"
     gene            7190..>8183
                     /locus_tag="VN627_07785"
     CDS             7190..>8183
                     /locus_tag="VN627_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXD03193.1"
                     /translation="MPLADPVPAAAVAPAASPDAVAVSIVMPCLNEAQSLAHCVANAR
                     EALDRIAVKYGLPGEIVIADNGSTDGSQAIAEALGARVVPVAAKGYGAALIGGFDAAR
                     GRYLVMGDADGSYDFTDAVAMIGRLEDGADLCMGSRFKGGIKPGAMPWKNRYIGNPLL
                     TGILNLFFRSGIDDAHCGLRAIRKEAYRSLGLESTGMEFASEMVIKASLKRIRMDEVP
                     ATLSPDLRDRAPHLRPWRDGWRHLRYLFMLSPTWAFGVPALLLAGIGAVILAIASLHA
                     LGVVPGPTHFGVSWTTLATFMVTSGHFAAIMALATHFYGVRQGYRRLRPWLARLE"
BASE COUNT         1325 a         2532 c         2962 g         1364 t
ORIGIN      
        1 cggatcgatc cggaggggca gaacttcagc cagctggcca agaccatcaa ttcactgcgt
       61 gaatccgggc gcaataccga ggagcttgac ggcaagtggt ggcaccgctt ctccggcgcc
      121 ctggccgcca tgctgatgcc cctgctcggc gcgaccgccg ccttcggcct cgcccgctcg
      181 ggcgctctgc tgatccgctc ggtgatcggg atggcgctgg ggttcgccta cttcgtcatc
      241 gacaatgcgg ccctggccat cggcaacttc ggtgggtacc cgccgctggt tgcggcctgg
      301 gcgccgttcc tcctgttctt ttgtatcggc gaggcggtgc tgattcggac cgaggaatag
      361 ggtggccggc gggtcttgcg aatgacttgc acttgcaggt gttggtcaac cggctgaatt
      421 tacagcgata gttagttgat aaagtacagc cgggcgctgc cacggcttgt gctttctgcc
      481 gtgcgcgcct gccggaatcg cgcttgagaa agaacagctt ggcttatgcc gccgagtggg
      541 gggtgtagga aaggcggttg gtgttttggg gagcggggtg cgtgggcgcg cgactgtgcc
      601 ctattgccct accgtcagcc gttcttcgcc gccacctccc catttgcgcc gttggcgtga
      661 ctggggagga tctcttccgg tgtgttggac tggtcaggct tcgcccggct tccatacgat
      721 cgtccgtgcc tcgcggcgga ccatggtgct tttggcaatc cgtccgatca gcatccacag
      781 gccggggtag gtcgctcccg cataggtcgc ctgggcgcca gcgtggtcgc tcaggcggcg
      841 gtgggcttcg gcgagcgagt ggtagggcag gctgggcagg aggtggtggg tggcgtggta
      901 gcgcaggccg accggggccc agagttccgc ccaaattcca ggcggcggga cgttgacgga
      961 atcgaggtac tgagcggtga cggtcatctg ctcgccctcg ttttgccaga gatgggcgac
     1021 gagggtgcgc agctggttga gcagggcgac gaccgaaacg acgcacaggc cggtgaccag
     1081 aggccgccag ccgtagcgga cggacagccc gaccagcgtc agtgcccaga gcgcgccgcc
     1141 cgtctcctgc cacagcacca tccgggcgaa ggcgccttcg ggggcgcggc ggcgaaaggc
     1201 ggggttgatc gagagggcgg aagcgcgctg ccacaccagc ttgcgcagcg gcgggatcag
     1261 ggcacccagg ggcaccagca cggcgaagcg gaacaggagc gcgaagggca gtgccagcgc
     1321 gacgaggatg aagccgggca gcgaccacgg tttcatcagc gcgaggggga gatattccgg
     1381 atcgtcggcc gtgccgtaac gggtgcgggc gtggtgcagg gtatggacgt tctcatacat
     1441 gaacgagggg gtgagcagcg gaatgccgac gaggacattc cacgcgaacc ggaagcccgg
     1501 cagggcatcg cggtggatgt gggtcagctc gtgaatgaac aggagggcgc ggtagagcgc
     1561 gagggcggca aggagcgcgc aggcgagcgc ggcccagccg gtgagcagga tcgcgccggc
     1621 aagcgcacca tagccgagga gggccgaggc cagcatgtcg ggccagtaga tcccggcgcg
     1681 ggcggtattg atgtcgcgcg tgagctcggc ggcggcgcgg agcatggcca tgtcgtcagc
     1741 cccgatgctc gcgggcttgc cagcggtcgg ggcggcggtc ttactggcgg gaaaagcagg
     1801 ggaattcatc cagatctttc cacatcggcg gcgccgtaac gccggtatgg gggcctcgct
     1861 ctcgcgttgc ccgacaatgc cacggttgac aacatgcctc ccgggcggac agggctcatt
     1921 gacctggttc aagtttacct ggggacaagg agagacgagc cgtggctgcc cctgagatag
     1981 tcgtttcccc cgtcacaacc aaggctgaac gcggcacatt cgtcgacctt gcctatcgcc
     2041 tcaatgccga tgaccccaat tgggtgccgc cgctgcggat ggaggcgatg gagctggtca
     2101 cccccggcaa gaaccccttc ttcgagcatg ccacggtcca gtatttcctc gcccggcgtg
     2161 gggccgaggt ggtcgggcgg atttcggcgc atatcgatca cctggccgtt gcgatgagcc
     2221 ccgagcaggg gatgggcccg ggcgtgggca actggggcat gcttgaggcg actgacgagg
     2281 ccgtggccca tgtgctgatc gccgcggcgg aagactggct gcgcgggcag gggatgaccc
     2341 gggtgctggc gccgctctcc atgtcgatct gggaagagcc ggggcaactg gtgtttggtt
     2401 tcgaccatcc gccgacggtc atgatgggcc accagggaga acgctaccag cgctggatcg
     2461 agagcgccgg cggctatgag ccggccaagc agctccacac ctacgagctc gacatcacca
     2521 aggaatttcc gccgctgatc cagcggatcg tggcctcggg cgagaagaac gcccgcatcc
     2581 gcatccgcaa taccaacaag aagaagttcg aggaggaggc gcggctgatc ctcgacatcc
     2641 tcaacgacgc ctggtcggac aactggggct tcgtcccctt cacggaaacc gagatcaagt
     2701 acgccggcaa gaagttcaag ccgatcgtcc acgaagacct gatcatggtc gccgaatacg
     2761 acggcgagcc ggtggccttc atgatgaccg tgccggacct taatgaagcg attctgccaa
     2821 tgggcggcaa gctgtttccg ttcaactgga tcaagctgct cggctggctg aggaagccgc
     2881 gcccgcggac catgcgggtg ccgctgatgg gcgtgcgcaa gaagctgcag agctcgcgca
     2941 tggcaagcca gctggccttc atgatgatcg agtacatccg ccgcaatgcc gtgagccgct
     3001 atggttcgac gcgcggcgag atcggctgga tcctcgagga caaccagggg atggtggcga
     3061 ttgccgaggc gatcaacagc cacgtgaaca agacctacgt gatctacggc aaggcgctgt
     3121 gaggcgccgc cggttctcgc tccgcttgaa aggaaccggg aggaaggggc cgtctggaga
     3181 gcgatgaaac atagccgcca ccccttgcgc ctctaccttg gccagcggat cgcgccgccg
     3241 cggttcctgg cctttctggt gctgctgccg ctggcgacct gggggcagca cgcgctggtg
     3301 ggaagcgact ggacggccgc gctggcgctg gggttcgact ttgccgcggc gctgttcctg
     3361 gtctcgctgc tgcccctgat cctgcgccat gaggccgacg cgattcgctg ccgggccgat
     3421 gccaacgatg ccaaccgggt ggtggtgctg gtgatcagct cgatgctgac gatcgtggtc
     3481 atggcggcga tcgccgggga actggcgggc gcgcgatcgg gccagctggc atcggtgatc
     3541 gtgctggtgg ccacgctggt gctgatctgg ctcttcgcca actcggtcta cgcgctgcac
     3601 tatgcgcacg agttctatac gcccgaggcc ggcgaccagg gcccgcgcga ccggggcggg
     3661 ctggattttc ccggcacgcc cgagccaggc taccgcgatt tcgcctattt tgcctttacc
     3721 ctgggcatga cattccagac ttcggacgtg gcgatcaccg ccagccgcat ccgcgcggtg
     3781 gcggtgctgc attcgttcgc ggccttcgtc ttcaacatcg gggtgatcgc cttcaccatc
     3841 aacgtgctgg gcggggcgag ctgaaaaggg cggcggactg ccgaatttcg accatagtcc
     3901 ctgttgcgat gcagcaggag caaggctaag cgccggattc tctccaccgg gcggcgcgct
     3961 gcgctgccgc gaaacgaagg ttgtccatgc gcatcgacat gatccccatc ggggaaaatc
     4021 cgcccgagag cctcaacgtc atcatcgagg tgccggtcgg cggtgagccg gtgaagtatg
     4081 aattcgacaa ggcctcgggc gcgctgttcg tggaccggat cctgcatacc ccgatgcgct
     4141 atccggccaa ctacggcttc gtgccgcaca cgctttcccc cgatggcgac ccgctcgacg
     4201 cgctggtgat cgcccgctcg ccgttcgtcc ccggctcggt cgtccgcgcc cggccgatcg
     4261 cggtgttgaa ccttgaggac gagcacggcg gcgacgagaa gctggtctgc gtgccggacg
     4321 acaagacctt cccctattac agcgacgtgg ccgagaaggg cgacctgccc gagatcgtgc
     4381 tccagcagat cgagcacttc ttcacccact acaaggacct ggaaaaggaa aagtgggtgc
     4441 gcgtgggcaa gtggggcggc gctgacgacg ccaagcggat cacgctggaa gcgatcgagc
     4501 gggccaagca ggcgaaagcg gcgtaaattt ggagagggaa aggccatccg gcctttccct
     4561 gccgcggccg cccagcccgc cgcgaaggcg gggggagttg cacggccgtt ccagttaccg
     4621 tcatgctgaa cttgtttcag catccatggc gcggcactcc ctccggatgc ggcagcgccg
     4681 gccttgcacc tacatcctcg ccagccagaa gggcggcacg ctgtacatcg gcgtgacctc
     4741 cgacctcccg gcgcgcatat ggcagcaccg gcatggcacg accggcggct tcgcttcgcg
     4801 gtaccgggtt taccgcctgg tccatgccga gtttcacgag gacatgcctt cagccatcgc
     4861 gcgggaaaag cagctcaaga actggcatcg tcagtggaag atcaacctca tcgaggcggt
     4921 caacccgcac tggctggatc tcgcccggaa gtgggggatt gccgaggcgt gatcccatgg
     4981 atgctgaaac gagttcagca tgacggggaa ttgagcaagg tcgtgtggtc cggcagtgcc
     5041 gcgccgagtc agccttccgc cgcctcctgc ttccaacccc agaacaggaa gcagggcatg
     5101 acgttcagcc attcgaaccc gctgtcgatc cgctggaagt ggcgggccat gaaatcgcgc
     5161 gccttggcgg tgaactggta gacgaggaag gcgccgccgg ggcgcaggac gcggtgggtc
     5221 gccgcggcga tggcggggcc gacgccttcg ggcagggtcg agaacggcag gccggagagc
     5281 acataatccg catggtcgtg cccgtgggcg cggacgatct cttcgacttc ggcggccgag
     5341 ccgaggaccg cggtaaagcg gctgtcggtg atcgcgcggc gcaggtagtc gatatagagc
     5401 gggttggtgt cgatcacgat cagctggccg tcacgccgca ggcgatcgag caccgggcgg
     5461 cagaaggtgc cgatgcccgg accatattcg acgaagagct tgcaattgtc ccagtccacc
     5521 gggccgagca tcttgcggac ggtgaagcgc gaggagggga tgatcgagcc gaccatcacc
     5581 gggtggcgca ggaagccttc gacgaagacg ccccatggcc ccagcatccg cttgacccgg
     5641 cgcttgatgg attgtggcag cgcctcgcgg gccagctcgg aagaggtcat ctgggcagta
     5701 tttcccggtc agtcgacggc ggcttaggcg gattgcaaat ttgccgcgcc ttggcaagcg
     5761 gggagccgcg cggagaggtc cgcgtgccat cccgccttgc gacaggccgt gggtgtgcgg
     5821 gggcaagccg ttggcatcat tcgcccgaag cggcgggttc ggtgggctcg gcggcggggc
     5881 tgtcggcggg tgaaggggtg gttccggcag gcgcgggggt gagcgcgccg gcgacgcggc
     5941 ggacagccgg aacgatccgg ggatagacgc cgcagcggca caggttggtg atggcggcgg
     6001 cgatgtcctc gtcggcggga tgcgcgttgc gggcgaggag gcccaccagc gccatggcga
     6061 tgcccggcgt gcagaagccg cactggatcg cctgttcatc gatcagggcc tggagcaccg
     6121 gatggcgcgg cacgccgggg gcgcggcggg cgaggccttc gatcgtggtg acgctgcggc
     6181 cttcggcctc ggccatggta agggtgcagg cgggcagcgg ctggccgtcg accagcaccg
     6241 tgcaagcgcc gcattcgccg acgccgcagg catatttggt gccggtgaga ttggccagat
     6301 cgcgcagggc ccacagcagc ggcatggcgg gatcggcggc gatttccacg ggctggtcgt
     6361 tgaggacgag gctggccatt gccgcgcatc ctagcgaggc ggatggcggt tgcacagcct
     6421 gcgacagggc cggggccgaa ggtcgaaggc gcccggcctg cagcgcggac gctgaacgcg
     6481 aaaggcccgc accggcaggg tgcgggcctt ccatcgcggc gatcgatcgt ggttcagccg
     6541 cccatcgagt gcttgagccg gctcatctcg tcgtggcccg aacgcaccga ttcccaggcc
     6601 tgctggacgg cgctgcgggc ctcgccggag agcccttcgt ccttgagcgc ggcttcgaac
     6661 ttgcccttga ggtaatcctc cccgcgttcg acctcgttga cgatggcctt gtcgtcgctg
     6721 ccgatgacgg cttgcttgag attgacgaac atgcggtggg cgccgccggc gatgctgccg
     6781 tcgtcggccg ggttgccgcc gaggcggcgg accgattcct ggaggtccgt cacggcgcgg
     6841 cggcgttcct gggcgcgggc ggtaaagcgc tgggcgagcg cgggatcgcg gatatcgccg
     6901 gcggaggtct cgtagccctc gatactgtcg atcagggtgg tgatgagggt gttgagggtg
     6961 gtgacgtcgc ctgtttcggc catgagtggg tactccgcat ggggttgttg tgggcgatca
     7021 accggccagc agggggcgtg gctccggggc cggacgccgg cgcggggcgg ctggtcgctt
     7081 tgccggcgcg ctggagcggg gaaaaagtgc cgcgcggcgg ccgcgcagcg agacttggcc
     7141 gatctgtttg gctaagaggc cctgcctgcc cggggaatga tgaccgccca tgccgcttgc
     7201 cgaccctgtt cctgccgccg ctgtcgcccc cgccgcgagc ccggatgccg tcgccgtctc
     7261 gatcgtgatg ccctgcctta acgaggcgca gtcgctcgcg cactgcgtgg ccaatgcccg
     7321 cgaggcgctg gaccggatcg cggtcaagta cggcctgccg ggcgagatcg tgatcgccga
     7381 taacggatcg accgacggca gccaggcgat tgccgaggcg ctgggcgcgc gggtcgtgcc
     7441 ggtggcggcc aagggctatg gcgcggcgct gatcggcggg ttcgacgccg cgcgcggacg
     7501 ctaccttgtc atgggcgatg ccgacgggtc ctacgatttc accgatgcgg ttgcgatgat
     7561 cgggcggctc gaggacgggg ccgacctgtg catgggttcg cgcttcaagg gcgggatcaa
     7621 gccgggggcc atgccctgga agaaccgcta catcggcaac ccgctgctga ccggcatcct
     7681 caacctgttc ttccgctccg ggatcgacga cgcgcactgc ggtctgcgcg cgatccgcaa
     7741 ggaggcctac cgctcgctgg ggctggaaag caccggcatg gaattcgcca gcgagatggt
     7801 gatcaaggcc agtctcaagc gcatccgcat ggacgaggtg cccgccaccc tgtcgcccga
     7861 cctgcgcgac cgcgcaccgc acctgcgccc gtggcgcgac ggctggcggc acctcaggta
     7921 cctgttcatg ctgagcccga cctgggcctt cggggtgccg gccctgctgc tggcggggat
     7981 cggcgcggtg atcctcgcca ttgcctcgct ccacgcgctg ggcgtggtgc ccgggccgac
     8041 ccatttcggg gtgagctgga cgaccctggc caccttcatg gtgaccagcg ggcatttcgc
     8101 cgcgatcatg gcattggcga cgcacttcta cggcgtgcgc cagggttacc gccggctgcg
     8161 gccctggctg gcgcggctgg aga
//
LOCUS       DATNBV010000325         5619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2744173,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000325 DATNBV010000000
VERSION     DATNBV010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2744173"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1303)
                     /locus_tag="VN627_07790"
     CDS             complement(2..1303)
                     /locus_tag="VN627_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HXD03194.1"
                     /translation="MPRFDSPALRQRLAAILGAVLLGLVAVGFARAGEWVQRWFDRAI
                     AGWPLLPLLLTPMVFALTVVATRRVAPDARGSGIPQVMVAAHAPAAPTSVRMLSLRGA
                     VVKIGGTLAMLAGGGAVGREGPTVQVSAALMVAVHRWLRVPLTSGVIIAGGAAGVAAA
                     FNTPVAGVAFAIEELASAFEQKMAGLVMLTVVAAGLVSLGLDGDYVYFGAMTQGLPLR
                     TMVLAALAAGLSGGLAGGLFARAVVALTLPPPGTRRARLRARPVLLAVLCGLLVALVG
                     LACGGVSWGTGYGATRALLGGGAVSPWFGPSRFVATLLASISGAPGGIFAPSLSVGAG
                     LGRLLADAFPGDSTGAVVMLGMIGYFAGVVRAPLTAVIIVMEMTANRTMILPLFATAL
                     VADQVSAWLQPDKLYHALARGFRPAAADPAGGAPDAALAPA"
     gene            complement(1418..1795)
                     /gene="rplL"
                     /locus_tag="VN627_07795"
     CDS             complement(1418..1795)
                     /gene="rplL"
                     /locus_tag="VN627_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HXD03195.1"
                     /translation="MADIAKLVEELSQLTVLEAADLAKALEEAWGVSAAAAVAVAAPA
                     GGGEAAAAAEEQTEFDVILTGDGGKKIQVIKEVRAITNLGLTEAKTLVESAPKAIKEA
                     VSKAEAEEIKKKIEEAGGTVEIK"
     gene            complement(1863..2375)
                     /gene="rplJ"
                     /locus_tag="VN627_07800"
     CDS             complement(1863..2375)
                     /gene="rplJ"
                     /locus_tag="VN627_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HXD03196.1"
                     /translation="MDRSQKAESVASLNATFAEVGVVVITRNLGMTVAQSTALRGKVR
                     EAGATYKVAKNSLAKLAANGTAYEGISDLFTGPTAIASSVDPVAAAKAVVDFAKTTDK
                     IEIVGGSLGSVVLNADGIKALATMPSLDEMRAKLIGLIQAPATKIAQLSTAPAAKLAR
                     VFQAYADKAA"
     gene            2784..3428
                     /locus_tag="VN627_07805"
     CDS             2784..3428
                     /locus_tag="VN627_07805"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXD03197.1"
                     /translation="MTATAELTAAPKLPFWRKPRVMDRVEQVAIVVMWIFLVQRVEIS
                     GNPYAPLLLISETAVAIFALIRRPTDKLSMELGDWLLAITATSAGLLIIPGVTLVPAL
                     APLGVFFAVGGNFVQAWAKLVLRRSFGVAPANRGIKLTGPYRYVRHPMYAGYLLVHVG
                     VMILMFSPINMVIYAIGWWAQILRILAEERLLSQDPEYAAYMTTVRWRLIPGVF"
     gene            3537..4286
                     /locus_tag="VN627_07810"
     CDS             3537..4286
                     /locus_tag="VN627_07810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD03198.1"
                     /translation="MTGGARGMGAATVRAYVAEGAQVVSLDLLEDEGMAVAEAANAAA
                     EAGSANFIACDVSSRASVDAAFAEAARRLGGLDVLAHVAGLERGDTAEDCDDATWDLM
                     LNVNAKGTMYTNQAAFRLMQDHGGQIINFGSAAGVKGMTNAVAYSASKGAVLAWTRAC
                     AQSWGRYNISVNAIAPAIWTPMYDAHRARMSNSELASHDEMMRMAVPLGGKLGDPVTQ
                     FAPVMVFMATDGARFITGQTLPVDGGVLMLT"
     gene            4618..>5619
                     /locus_tag="VN627_07815"
     CDS             4618..>5619
                     /locus_tag="VN627_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HXD03199.1"
                     /translation="MATKPQSSGVRNARSKRIRKIFGDIHEVVQMPNLIEVQRESYEQ
                     FLRSDPSIGYVSGLEKTLRSVFPIRDFAGTAELDFIHYELEPPKYDVTECRQRGITYA
                     APMKVTLRLIVFEVDSETETRSVLDIKEQDVYMGDMPLMTENGTFFINGTERVIVSQM
                     HRSPGVLFDHDRGKTHSSGKYLFAARVIPYRGSWLDFEFDAKDIVNVRIDRKRKLPVT
                     ALLHALGLDDEAILNHFYQTVTWDRGSGGWQVPFVVEQWRGAKPAFDIVDAKSGEVIF
                     AAGTKISPRAANKAEKDGLSQLLIPTEEIFGRYSARDLIDESTGRIWIEAGDEVTPEN
                     "
BASE COUNT          934 a         1816 c         1960 g          909 t
ORIGIN      
        1 gtcaggccgg ggcgagggcg gcgtccggcg ccccgcccgc cgggtcggcg gccgccgggc
       61 ggaagccacg ggcgagcgcg tggtagagct tgtcgggttg gagccaggcg ctgacctggt
      121 cggcaacgag agccgtggcg aacagcggca ggatcatggt gcggttggcg gtcatctcca
      181 tgacgatgat caccgccgtc agcggcgcgc ggaccacgcc ggcaaaatag ccgatcatgc
      241 ccagcatgac gaccgcgccg gtgctgtcgc ccgggaaggc gtcggcaagg agccggccga
      301 gcccggcacc aaccgagagc gacggggcga agatgccgcc cggggcgccg gaaatgctgg
      361 cgagcagggt ggcgacgaag cgcgacgggc cgaaccacgg cgaaaccgcg ccgccgccga
      421 ggagggcgcg ggtggcgccg tagccggtgc cccagctgac tccgccgcag gcgaggccga
      481 cgagcgccac caggagaccg cacagcactg ccagcaggac agggcgggcg cgcaggcggg
      541 cgcggcgggt gccgggcggg ggcagggtaa gggcgacgac ggcgcgggcg aagaggccgc
      601 cggctagccc tcccgacaag cccgcggcga gcgcggcgag gaccatggtg cgcagcggca
      661 ggccctgcgt catcgcgccg aagtagacat agtcgccatc gagcccaagg cttacgaggc
      721 cggcggcgac cacggtcagc atgacgaggc cggccatttt ctgctcgaag gcactggcca
      781 gttcctcgat agcgaaggcg accccggcaa cgggggtgtt gaaggcggcg gctacccccg
      841 cggcgccccc ggcaatgatc accccgctgg tcagcggcac gcgcagccag cggtggaccg
      901 cgaccatcag tgcggcgctg acctggacgg tcggcccttc gcgtccgacc gccccgccgc
      961 ccgcgagcat ggccagcgta ccgccgatct tgacgaccgc gccacgcagc gagagcatcc
     1021 gcaccgacgt tggtgccgct ggggcatggg cggcgaccat gacctgtgga atgcccgagc
     1081 cccgcgcatc gggtgccacc cggcgggtgg cgacaacggt gagcgcgaaa accatggggg
     1141 tgagcagcag cggcagcagc ggccagccgg caatggcccg gtcaaaccag cgctgaaccc
     1201 attcaccggc gcgggcaaag ccgaccgcga cgaggccgag caggaccgcg cccaggatcg
     1261 cggcgaggcg ctggcgcagg gcggggctgt cgaaccgggg catgggttta tggcctagtc
     1321 gttcgtcgcg cgggcgcaag tggttgccgg gatgcaaaaa gggcggcccc aggggagccg
     1381 ccctttctgt aatctctcgg gaagaacccg aagaagctta cttgatctcg acggtgccgc
     1441 cggcttcctc gatcttcttc ttgatctctt cggcttcggc cttcgagacg gcttccttga
     1501 tcgccttcgg agccgattcc acgagggtct tggcttcggt cagaccgagg ttggtgatcg
     1561 cgcggacttc cttgatgacc tggatcttct tcccgccgtc gccggtcagg atgacgtcga
     1621 attcggtctg ctcttcggca gcggcggcgg cttcgccacc accggccggg gcggccacgg
     1681 cgacggcagc ggcggcgctg acgccccacg cttcttcgag ggccttggca aggtcagccg
     1741 cctcgaggac ggtcagctgc gacagttctt caacaagctt ggcgatatcg gccatgatgc
     1801 ttcactcctg aattgatgga ccggaaaggc ggcgcccttc cgggaattcg cttgaacagt
     1861 ctttaggcgg ccttgtcggc gtaggcctgg aagacgcggg ccagcttggc cgccggagcg
     1921 gtcgacagct gggcgatctt cgtggccgga gcctggatga ggccgatgag cttggcgcgc
     1981 atttcgtcca gcgacggcat cgtggcgagc gccttgatcc catccgcgtt gaggacgacc
     2041 gaacccagcg agccaccgac gatctcgatc ttgtcggtgg tcttcgcgaa gtcgacgaca
     2101 gccttggcgg cggcaaccgg atccaccgac gaagcgatgg cggtcgggcc ggtgaagaga
     2161 tcggagatcc cttcgtaggc ggtgccgttg gcggcaagct tggcaagact gttcttcgca
     2221 accttgtagg tcgcgcccgc ttcacgaacc ttgccgcgca gggcggtgga ctgggccacc
     2281 gtcatgccga ggttgcgggt gatcaccacc acgccgacct ctgcgaaagt cgcgttcagc
     2341 gatgcgaccg attcggcttt ctgcgaacga tccatgcctt actccttcac aatggccacc
     2401 tgcacaggca ggcggccggc tacgttgtcc cgcctgccgg ggcagacggg gcgagtccgt
     2461 tgagagggga aggagcacgc cggagcgtga aagcaccacg ggcctggggc ggcgtgggcg
     2521 gatagactct tttccccgtc taggctggaa attaagcgag ggcagacccc tggcaccaac
     2581 tgtctcggac ggatgactgg cgggacgaat ccagccggtc ggggcgggca tttacgcgag
     2641 ggcgggggaa agtcaaggtt gggagtcctt gatagggtcc gccctttggc atccgccaag
     2701 ggcttgcggc accagcccgc tccccctcaa ttctttgtta accctgttct ggcactccgc
     2761 aattgtactt gtgggggccg agaatgaccg cgactgccga gctgaccgct gcacccaagc
     2821 tccccttctg gcgcaagccc cgggtcatgg accgggtgga gcaggtggcg atcgtcgtca
     2881 tgtggatctt cctcgtccag cgggtcgaga tttccggcaa tccctatgcg ccgctgctgc
     2941 tgatctcgga aaccgcggtg gcgatcttcg cgctcatccg gcgcccgacc gacaagctgt
     3001 cgatggaact gggcgactgg ctgctggcga tcaccgcgac cagtgctggc ctgctgatca
     3061 tacccggcgt gaccctggtg ccggccctgg cacccctggg cgtgttcttc gcggtcggcg
     3121 gcaatttcgt gcaagcctgg gccaagctgg tgctgcgccg ctcgttcggg gtggctccgg
     3181 ccaaccgcgg gatcaagctg acgggcccct accgttacgt gcgccacccg atgtatgcgg
     3241 gctacctgct ggtgcatgtc ggcgtgatga tcctgatgtt ttcgcccatc aacatggtga
     3301 tctatgcgat cggctggtgg gcgcagatcc tgcgcatcct ggccgaggaa cgcctgcttt
     3361 cgcaggatcc cgaatatgcg gcctatatga cgacggtgcg ctggcggctg atccccgggg
     3421 tgttctgagc ttgcccgcga cagtctgatc gggcatctcc ccggccgtga agacgggcgc
     3481 caggcccgtc ccggcgagag gacaggggaa tgcagcttct tggacgaagg atcatcgtga
     3541 ccggcggcgc ccgcggcatg ggcgcggcga cggtgcgggc ctatgtggcc gaaggggcgc
     3601 aggtggtttc gctcgatctg ctcgaggatg agggcatggc cgtggcggaa gcagccaacg
     3661 ccgctgcgga agccggctcg gcaaatttca tcgcctgtga cgtgtcctcg cgcgcatcgg
     3721 tggacgcggc ctttgccgag gccgcgcggc ggctcggcgg gctcgacgtg ctggcgcacg
     3781 tggcgggcct cgagcgcggc gatacggcgg aagactgcga cgacgccacc tgggacctga
     3841 tgctgaacgt caacgccaag ggcacgatgt acaccaacca ggccgctttt cggctgatgc
     3901 aggaccacgg cggccagatc atcaacttcg gctcggccgc tggcgtgaag ggcatgacca
     3961 atgccgtggc ctattcggcc tccaagggcg cggtgcttgc ctggacccgg gcctgtgccc
     4021 aatcgtgggg acgctacaat atctcggtca acgccattgc tccggccatc tggaccccga
     4081 tgtatgacgc acaccgcgcc cggatgagca attcggagct ggcctcccac gacgagatga
     4141 tgcgcatggc ggtgcccctg gggggcaagc tgggcgatcc ggtgacgcag ttcgccccgg
     4201 tcatggtgtt catggctacc gacggcgcgc ggttcatcac cgggcagacc ctcccggtcg
     4261 acggcggggt gctcatgctg acctgaacgc ggcgcccggg cgccaccctc gccgaaccgg
     4321 gccgggggcg cggtgaagcg ggcgcggcgg agcgggtccg atacgttgac tttccacagg
     4381 ttcgcgccta tctgcgccgt tcgctcgagg gcttcgagca aggccgtgct tgcgcgggtt
     4441 cggccaggga aggaagcctt tcgggttttc atgcgacgca aaccggccgg gaagcggtct
     4501 ccgtgagggg attcgcgcgc ccgcctgtct tgcgttcatg gccaccgaat cgcgttcgtg
     4561 gcgtgacttt ctggccgggc gctgcctggc cgtacgagaa gaggcaaaag acccctcatg
     4621 gcgaccaagc cccaatcctc gggtgtgcgc aatgcacgct ccaagcggat ccgcaagatc
     4681 ttcggcgaca tccacgaagt ggtgcagatg ccgaacctga tcgaggtcca gcgcgagagc
     4741 tacgagcagt tcctgcgctc cgacccctcg atcggctatg tctcgggcct ggaaaaaacc
     4801 ctgcgttcgg ttttcccgat ccgcgacttt gccggcaccg ccgagctgga cttcatccac
     4861 tacgagttgg agccgccgaa gtacgacgtg accgagtgcc gccagcgcgg cattacctat
     4921 gccgccccga tgaaggtgac cctgcgcctg atcgtgttcg aggtggacag cgaaaccgaa
     4981 acccgctccg tgctcgatat caaggagcag gacgtctaca tgggcgacat gccgctgatg
     5041 acggagaacg gcacgttctt catcaacggc accgagcgcg tgatcgtgtc gcagatgcac
     5101 cgttcgccgg gcgtgctgtt cgaccatgac cgcggcaaga cgcactccag cggcaagtac
     5161 ctcttcgctg cccgcgtgat cccctatcgc ggttcgtggc tggacttcga gttcgacgcc
     5221 aaggacatcg tcaacgtccg catcgaccgc aagcgcaagc tgccggtgac cgcgctgctc
     5281 cacgcgctgg gtctggatga cgaggcgatc ctcaaccact tctaccagac ggtgacctgg
     5341 gaccgtggca gcggcggctg gcaggtgccc ttcgtggtcg agcaatggcg cggcgcaaag
     5401 ccggccttcg acatcgtcga cgccaagagc ggcgaggtga tcttcgccgc cggcaccaag
     5461 atctcgcccc gcgccgccaa caaggcggag aaggacgggc tttcgcagct gctgatcccg
     5521 accgaggaaa tcttcggccg ctattcggcg cgcgacctga tcgacgagag cactggccgc
     5581 atctggatcg aggccggtga cgaggttacc cctgagaac
//
LOCUS       DATNBV010000326         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1774917,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000326 DATNBV010000000
VERSION     DATNBV010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774917"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /gene="ribB"
                     /locus_tag="VN627_07820"
     CDS             <1..667
                     /gene="ribB"
                     /locus_tag="VN627_07820"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HXD03200.1"
                     /translation="TQLQSEERFTMSISPRIEKRITAACDAMRAGLPVVLLDDETRED
                     EADLIAAAETLSHDVMARLIRDCSGIVCLCLDESLVRQLDLPPMAAKNETRFGTAFTV
                     SIEAKLGVTTGVSAADRLTTVRAAIADGARPDHLARPGHVFPLRAMPGGVLTRRGHTE
                     GAVDLARLAGLRPAAVLCELMDRKGEMLRGKRAKAYARRNGFPVLTIADLAGHLGALD
                     IAA"
     gene            819..1472
                     /locus_tag="VN627_07825"
     CDS             819..1472
                     /locus_tag="VN627_07825"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HXD03201.1"
                     /translation="MQLGGRVGSLGAQPGPQGGQGAQVQQPHFIRMYRDTFSREQCDE
                     VIRRFEADERKHSSTTATRDKPRLRTGTMLQIGDLPEWQDIAQAWSQALEKNLQAYAE
                     AFPTLKQLVSTPATKRTPPLLERVEPGQGFGLHIDASVAGTHDRMVAVLMYLKDVEEG
                     GFTEFPFQSIRVRPRAGLMLLFPPYWTHPHQGVSPVSGVKYNLTSYVVVDNNFRPPR"
     gene            complement(1484..>2075)
                     /locus_tag="VN627_07830"
     CDS             complement(1484..>2075)
                     /locus_tag="VN627_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03202.1"
                     /translation="AAPSPTRTQMDGASISLPFLFLAAVITLSATISTILSVHLLTIL
                     QARDIALAAAVALGALVGPSQVGARFIEMMIARYHHPIWTMLASAVFMAIGIGTLWAG
                     LPILSVALVFYGAGIGIESIARATLPLSLFDPANYARIMGKLAFPSLIAQAAAPSIGT
                     LLMLHLGPGGTLLTLTILAAVKVGLVGALYICVRNR"
BASE COUNT          360 a          674 c          737 g          304 t
ORIGIN      
        1 aacccaactc cagagcgagg agcgtttcac catgtctatt tccccccgga ttgaaaagcg
       61 gatcacggcc gcgtgcgatg ccatgcgcgc agggctgccg gtcgttctgc tggacgacga
      121 gacccgcgag gacgaagccg atctgatcgc ggcggcggag accctgtcgc acgacgtgat
      181 ggcgcggctg atccgcgatt gcagcggcat cgtctgcctg tgcctggatg aaagcctggt
      241 gcgccagctc gatctgccgc cgatggcggc gaagaacgag acgcgcttcg gcacggcttt
      301 caccgtctcg atcgaggcca agctcggcgt cacgaccggc gtctcggcgg ccgaccgcct
      361 gacgacggtg cgcgcggcga tcgcggacgg cgcgcggccc gaccatctgg cgcggcccgg
      421 gcacgtgttc ccgctccgtg ccatgccagg cggcgtgctg acgcggcgcg gccacaccga
      481 aggggcggtc gacctggcgc ggctggccgg gctgaggccc gccgccgttc tgtgcgagct
      541 gatggaccgc aagggcgaga tgctgcgcgg caaaagggcc aaggcctatg cgcggcgcaa
      601 cggcttcccg gtgctgacca tcgcggacct ggcggggcat ctcggcgcgc tcgacatcgc
      661 tgcttagatc gcgcaactcc ctcccccgag ccgggggagg gagttccagt ttgtgctccg
      721 tgcgcccggt tacacggctg tgcccgtttc tgctatggtt gccaaacgga acacatcgac
      781 aacctgggac gcgggcgatg gcggcgaagg atggtggggt gcagttgggc ggccgggtcg
      841 gctccctggg cgcgcagccg gggccgcagg gcggccaggg cgcgcaggtc cagcagccgc
      901 acttcatccg catgtatcgc gacaccttca gcagggagca gtgcgacgag gtgatccgcc
      961 gcttcgaagc ggacgagcgc aagcacagct ccaccaccgc gacccgggac aagccgcgcc
     1021 tcaggaccgg caccatgctg cagatcgggg atctgcccga gtggcaagac atcgctcagg
     1081 catggtcgca ggcgctggag aagaacctgc aggcctatgc cgaggccttc cccaccttga
     1141 agcagctcgt ctccaccccc gccaccaagc gcacgccgcc gctgctggag cgggtcgagc
     1201 cgggccaggg tttcggattg cacatcgatg cctcggtcgc cggcacccat gaccgcatgg
     1261 tggccgtgct gatgtatctg aaggacgtgg aggaaggggg cttcaccgag ttcccgttcc
     1321 agtcgatccg cgtccgccca cgcgcgggcc tgatgctgct gttcccgccc tattggacgc
     1381 acccgcacca gggggtgagc ccggtcagcg gcgtcaaata caatctcacc agctacgtgg
     1441 tggtggacaa caatttccgc ccgccgagat agcgcgcctc gagttaccgg ttgcgcacac
     1501 agatgtacag cgcgccgacc aggccgacct tcacggcggc gaggatggtg agggtaagca
     1561 gcgtgccgcc ggggccgaga tgcagcatca ggagcgtgcc gatgctcggg gccgcggcct
     1621 gggcgatcag gctggggaag gcgagcttgc ccatgatgcg cgcatagttg gcgggatcaa
     1681 acaggctgag cggcagggtc gcgcgcgcga tggattcgat gccgatgcca gcgccataga
     1741 acaccagcgc gacggacagg attggcagcc ccgcccacaa agttccgatg ccgatggcca
     1801 tgaacacggc cgaagccagc atggtccaga tcggatggtg atagcgcgcg atcatcatct
     1861 cgatgaagcg cgctcccacc tgcgacggac ctaccagcgc gcccagcgcg acggcggcgg
     1921 ccagcgcgat gtcgcgcgcc tgcaggatgg tcagcagatg cacggagagg atggtggaga
     1981 tggtcgcgct gagggtgatg acagcggcca ggaacaggaa gggcagcgag atcgatgcgc
     2041 catccatttg ggtccgcgtc ggtgacggcg cggct
//
LOCUS       DATNBV010000327         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4661532,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000327 DATNBV010000000
VERSION     DATNBV010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4661532"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VN627_07835"
     CDS             complement(<1..457)
                     /locus_tag="VN627_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit II"
                     /protein_id="HXD03203.1"
                     /translation="MDPPRSSAMIRPVCRRGSAALPLVALLSACSGRSIFDPVGPVGA
                     DDSKILIDATLIMLAIVVPTILLALWMAWRYRASNTSAEYLPNWSYSGRIEAVVWAIP
                     TLTIMFIGGVIWIGSYRLDPFRPLASPVPPLEVQVVALDWKWLFIYPQQG"
     gene            complement(597..851)
                     /locus_tag="VN627_07840"
     CDS             complement(597..851)
                     /locus_tag="VN627_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03204.1"
                     /translation="MPLAQVTVLLPKLPDGGEVSRVMAAKAGVAAASNGKAAKDTNLF
                     IIGDSFLFIWQSAIVALALNGALSLCFQRLMPICDERLAP"
     gene            751..1281
                     /locus_tag="VN627_07845"
     CDS             751..1281
                     /locus_tag="VN627_07845"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HXD03205.1"
                     /translation="MLAAATPAFAAITLDTSPPSGNFGNSTVTCASGTFSCAFSDTFT
                     FVTPDGFNSVGATLSSGPATDAATTLIFGTLGLLSGVTLNGQMFDLKLAGDYNFATLD
                     PIPLTAGATNTLVVSGIAGQSGGGSYAGTLTFGNTAAVPEPATWATMLLGLAAAGWML
                     RRKRGTTVQAPRISFA"
     gene            complement(1411..1971)
                     /locus_tag="VN627_07850"
     CDS             complement(1411..1971)
                     /locus_tag="VN627_07850"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HXD03206.1"
                     /translation="MPAHLITTLVHRLGPAGVALGAGLEGETAVVIGGTAAHHGLFNP
                     VAAALAAWAGSFVADQLVFWLSREQRHRLFVQKLAAEPKVGRALGIIDKHPRLFCCAF
                     RFVYGFRIAGPAAVGLSAVPGRLFVACNAVSAAIWAALFTFICYHFGNALLAAIRPFF
                     HVPHVGVELAVAIVAIGLLWLARPKR"
     gene            complement(2012..>2690)
                     /locus_tag="VN627_07855"
     CDS             complement(2012..>2690)
                     /locus_tag="VN627_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXD03207.1"
                     /translation="LLHGGEHLWSLCVEMQFYLLAALLCLAFGHRRGLLALPVLCVAV
                     TIGRVWAHEPISIITWQRIDEILAGSTLALAYRGRFGDLSHLFRLPLWVGIPALYLTS
                     APLFPPAEYLRPYVAALLVGSTLYAAPGPVHRLLTTRPMLLIAEMSYAIYVIHGVLTA
                     TWLGTPPHKYPKRPLLALATFLLAWLSTRYFERPVMRWARRLPLGQAPAPATAPPRRG
                     DGAVPGA"
BASE COUNT          508 a          873 c          920 g          389 t
ORIGIN      
        1 ccccctgctg cgggtagatg aacagccact tccagtcgag cgcgacgacc tgcacctcga
       61 gcggcggcac gggcgaggca agcgggcgga atgggtcgag ccgataggag ccgatccaga
      121 tcacgccgcc gatgaacatg atggtgagtg ttggaatcgc ccagaccacc gcctcgatcc
      181 ggccggagta ggaccagttc ggcaggtatt cggcgctggt gttggaggcg cggtaccgcc
      241 aggccatcca caaggcgagc aggatggtcg gcaccacgat tgccagcatg atcagcgtcg
      301 catcgatcag gatcttgctg tcatcggcgc cgaccgggcc gaccggatcg aagatgctgc
      361 gccccgagca ggcggagagc aacgccacca gcgggagcgc tgccgaaccg cgccggcaga
      421 ccggacggat catagcggaa gaccgtggcg ggtccatgcc cactcctact cgcagcgccg
      481 gtcacaacgt gccgttccag caggagttcc gcgattattt gtcgctgctg ccaccaggcg
      541 ggaacaagcc gtcccacacc gggattgggc acaggcgtgc gcacgattgc agcatcctaa
      601 ggggccagtc gttcatcgca aattggcata agtcgttgga aacaaaggct taacgcgccg
      661 ttaagcgcca gggcaacgat cgccgactgc cagatgaaca ggaaggaatc ccctatgata
      721 aagagattgg tatccttggc cgccttgcca ttgctggcgg ccgccacccc agcctttgcg
      781 gccataaccc tcgatacctc gcctccatcg ggcaacttcg gcaacagcac ggtgacctgc
      841 gccagcggca ccttcagctg cgccttctcc gataccttca ctttcgtgac acccgatggc
      901 ttcaattcgg ttggcgccac gctctcgtca ggcccggcaa cggacgccgc gacgacgctg
      961 atcttcggca cgctggggct gctttcaggc gtgacgctca acgggcagat gttcgacctg
     1021 aagcttgccg gcgattacaa cttcgcaacg ctcgatccga tccccttgac cgcgggtgct
     1081 accaacacgc tcgtcgtctc gggtatcgcc gggcagagcg gcggcgggtc ctacgcgggc
     1141 acgctgactt tcgggaacac ggccgccgtg cccgaaccgg caacctgggc taccatgctg
     1201 ctcggccttg ctgccgcagg ttggatgctg cgccgcaagc gggggacgac cgttcaggca
     1261 ccgcggatct cgttcgcctg actcgggctg gcttgcttcc ggatctcacc tcgtcggctt
     1321 agccccgttc cgcgaagttc tcctgcccat cgggccctgc gtcgccgaag ccaaggcggt
     1381 cgcggggcct tttgccgtgc gggcgggctc ctagcgtttc gggcgggcca gccaaagcaa
     1441 gccgatggcc acaatggcga ccgccagttc cacgccgaca tgcggcacgt ggaagaacgg
     1501 gcggatcgcg gcgaggaggg cattgccgaa gtggtagcaa atgaaggtga acagggccgc
     1561 ccagatcgcg gcggaaacgg cattgcaggc cacgaacaac cgtcccggga cggccgagag
     1621 gccgaccgct gccgggcctg cgatgcggaa cccgtagacg aagcggaaag cgcaacagaa
     1681 cagccggggg tgcttatcga tgatgccgag agcgcggccc accttcggct cggcggcgag
     1741 cttctgcacg aagagccggt gacgctgttc gcggcttagc cagaacacca gctggtcagc
     1801 cacgaacgag cccgcccagg cggcaagggc ggcggcgacg ggattgaaca ggccgtggtg
     1861 ggcggcggtc ccgccaatga ctaccgccgt ctccccctcc agccccgcgc cgagcgccac
     1921 tcccgccggg ccgagccggt gaacgagggt tgtgatgagg tgcgcgggca tgggcagctt
     1981 gttacgtcac gccaagttcg ttcgccatcc gtcacgcacc cggcactgcg ccatcgccgc
     2041 ggcgcggggg tgcagttgcg ggcgcaggcg cttggccgag cggcaggcgg cgcgcccagc
     2101 gcatcaccgg ccgctcaaag taacgagtgg agagccaggc cagcaggaag gtggccagtg
     2161 ccagcaaggg gcgcttggga tacttgtgcg gcggcgtacc cagccaggtg gcggtcagca
     2221 cgccgtggat cacgtagatc gcataggaca tttccgcgat gagcagcatc ggccgggtgg
     2281 taagcagccg gtgcacgggc ccgggcgcgg catagagggt ggagccgacg agcagcgcgg
     2341 cgacataggg ccgcagatat tcagccggcg gaaacagcgg cgcgctggtg aggtaaagcg
     2401 cagggatacc cacccatagc ggcaggcgga acaggtggct gaggtcgccg aagcggccgc
     2461 gataggcgag ggccagggtc gaaccggcga ggatctcgtc gatccgctgc caggtgatga
     2521 tgctgatcgg ctcgtgcgcc cagacccgcc cgatcgtaac cgccacgcac agcaccggca
     2581 gggcaagcag gccgcgccgg tggccgaagg cgaggcacag cagggcggcc agaaggtaga
     2641 actgcatttc cacgcacagg gaccacaagt gctccccgcc atggagcagc
//
LOCUS       DATNBV010000328         2143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1775788,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000328 DATNBV010000000
VERSION     DATNBV010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775788"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VN627_07860"
     CDS             complement(<1..1052)
                     /locus_tag="VN627_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXD03208.1"
                     /translation="MLTRLSIRNIVLIEALDLEFGRGLGVLTGETGAGKSILLDALGL
                     VLGNRADSGLVRAGEDMASVTGAFEFSTLPPGIAAVLAEAEVEVEPGEPLLVRRQLKA
                     DGGSKAFINDQPVGAALLRELAPFLVELHGQHDDRGLVNPRGHRALLDRYAGSDVVGI
                     EAAWKAWRQAEDRLAVARGRVAQASADQDLLLAHLAELTELSPEVGEEEELAEARSAM
                     QKGEKLADDLAALQHLWAGSDSALAALRSAARRLDRIAGEHPLLAEALESLDRSVIEA
                     SEAEDRLSRAAEALAHDPQALDRIETRLFELRAAGRKHGCAVDELPDRMLAMRAQLDA
                     IESGEAEIGGLERAAH"
     gene            complement(1210..1752)
                     /locus_tag="VN627_07865"
     CDS             complement(1210..1752)
                     /locus_tag="VN627_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03209.1"
                     /translation="MADIASLIVLAAGAAHEAEVEPSALGLAPGAWVALSMAVLIAIA
                     LFLKVPKAITGGLDKGIAEIKHQLEEAKALRAEAEVLRKEYADKIANAEREAAEMLAH
                     AQHEAEAIVAKAEADTTDLIARREKMASEKIAAAELATVQDLRNKAAAAAAAAAGTLI
                     RKGHDAAADKGLVDQAIAGL"
     gene            complement(1745..>2143)
                     /locus_tag="VN627_07870"
     CDS             complement(1745..>2143)
                     /locus_tag="VN627_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HXD03210.1"
                     /translation="GMVPKVMATVESRDRQIAADLAAAEAARAAAEAEQASWSSAAAK
                     QREEAHGVIAKAKAEAAKAAEVRLAAAGATIDTRVAEADARIAAARNSALGEIETVAA
                     EAAADIAARIAGLKVTAKAGAAAVKEALHG"
BASE COUNT          305 a          777 c          709 g          352 t
ORIGIN      
        1 tcatgggccg cccgctccag cccgccgatc tccgcctcac cgctctcgat cgcatcgagc
       61 tgggcgcgca tcgccaacat ccggtcaggc agttcatcca ccgcgcagcc gtgcttgcgc
      121 ccggcagccc gcagttcgaa caggcgcgtc tcgatccggt caagcgcttg ggggtcatgg
      181 gccagcgcct cggccgcgcg gctgagccga tcctccgcct cgctcgcctc gatcaccgaa
      241 cggtcaagcg attcaagggc ttcggcgagc agcggatgct ctcccgcgat ccggtcgagc
      301 cgccgcgcgg cagagcgcag ggcggccaag gccgaatccg aacccgccca caggtgctgg
      361 agagcggcga ggtcatcggc cagcttttcc cccttctgca tggcggagcg cgcctcggcg
      421 agttcctcct cctcccccac ttccggggaa agctccgtta attcagccag atgggccagc
      481 agaagatcct gatcggcact ggcctgcgcc acccgccccc gggccacggc cagccggtct
      541 tccgcctgcc gccatgcctt ccacgccgcc tcgatcccga ccacgtccga gccggcataa
      601 cggtccagca aggcccggtg accacgcggg ttgacgaggc cgcgatcgtc atgctgcccg
      661 tgcagttcaa cgaggaacgg cgccagttcg cgcagcaggg ccgctcccac cggctggtcg
      721 ttgatgaagg ctttggagcc gccgtcggcc ttcagctggc ggcggaccag cagcggttcg
      781 cccggctcca cttccacctc cgcctcggcc aggacggcag cgatgccggg cggcaaggtg
      841 ctgaattcaa aggcaccggt aacgctggcc atgtcttccc cggcgcgcac caatccgctg
      901 tcggcgcggt tgcccagtac cagcccgagc gcatccagca ggatcgactt gcccgccccg
      961 gtctcaccgg tcagcacgcc cagcccgcgg ccgaactcga ggtccagtgc ctcgataagc
     1021 acgatgttgc ggatcgaaag gcgggtcagc atcgctcggc cctgggtgtc ctcggcggat
     1081 gttcgcgtga cgttctagcc aagcccgggc ccccttgtca cgagggccca tgcgcctgcc
     1141 agaaattttc ccggcaaaag aaaacccccg ccgaagcggg ggttccttgg tcacgccctg
     1201 acgggacgct cagaggccag cgatcgcctg atcgaccagc cccttgtcgg cagccgcatc
     1261 gtggcccttg cggatcagcg tgcccgcagc agcggcggcg gccgcggcgg ccttgttgcg
     1321 caggtcctgc accgtagcca gttcggcggc cgcgatcttt tccgaggcca tcttctcgcg
     1381 gcgggcgatc aggtcggtgg tgtcagcctc ggccttggcg acgatagctt cggcctcgtg
     1441 ctgggcatgg gccagcattt cggcagcctc acgctcggca ttggcgatct tgtcggcata
     1501 ttccttgcgc aggacttccg cctcggcgcg cagggccttg gcctcttcaa gctggtgctt
     1561 gatctcggca atgcccttgt cgagcccgcc ggtgatcgcc ttgggcacct tgagaaacag
     1621 agcgattgcg atcagcaccg ccatcgagag cgctacccag gcgccgggag cgagaccgag
     1681 cgcgctcggc tccacttcgg cttcatgggc cgcaccggcg gcgagcacga tgaggctggc
     1741 aatgtcagcc atggagggcc tccttgacgg cagcggcgcc ggccttggcg gtcaccttca
     1801 ggcccgcgat gcgggcggcg atatcggccg cagcctcggc ggcgacggtc tcgatctcgc
     1861 ccagcgccga attgcgggcc gcggcgatgc gggcatcggc ttcggcgacg cgggtgtcga
     1921 tcgtggcgcc ggcggcggca aggcgcactt ccgcggcctt ggccgcttcg gccttggcct
     1981 tggcgatcac gccatgggcc tcctcgcgct gctttgcggc agcagaggac cacgaggcct
     2041 gttcggcttc ggcggcggcc cgggcggctt cggccgcggc gaggtcggct gcgatctgcc
     2101 ggtcacgcga ttcaacggtc gccatgacct tggggaccat gcc
//
LOCUS       DATNBV010000329         4192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2107881,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000329 DATNBV010000000
VERSION     DATNBV010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107881"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VN627_07875"
     CDS             complement(<1..222)
                     /locus_tag="VN627_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HXD03211.1"
                     /translation="MPVSFEPVGESAADMLVASCMAAAAQGDLSAYFDLGVAYSTGSH
                     GAAIDLIEAHKWFNLAAVHGYDDASQCRAD"
     gene            530..988
                     /gene="dksA"
                     /locus_tag="VN627_07880"
     CDS             530..988
                     /gene="dksA"
                     /locus_tag="VN627_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HXD03212.1"
                     /translation="MPVSLADDIDILVKAKRSIAGDYLPSDDEPYMGEAQQDYFRRLL
                     QAWKKAILDSSADTLQQLQDGPIREPDLNDRASSETDWGIELRTRDRQRKLIAKIDSA
                     LRRIEEGEYGYCEVTGEPIGLGRLIARPIATMTVEAQEAHERREKISRDD"
     gene            1109..1477
                     /locus_tag="VN627_07885"
     CDS             1109..1477
                     /locus_tag="VN627_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXD03213.1"
                     /translation="MSDSDNRNISRDSLFILAELRIDGRGDEHRVKVRNLSAGGMMAE
                     GPARVARGERVEVNLRNVGWVEGSVAWVQENRFGIAFAEDLDPSVIRVPVAASADQTP
                     RQLKNIYASAPAPGPARKIF"
     gene            complement(1799..2071)
                     /locus_tag="VN627_07890"
     CDS             complement(1799..2071)
                     /locus_tag="VN627_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03214.1"
                     /translation="MLPGSPQGHRPAAGTIAQEAEFRLGQQLQKVGNGTAETDFENTA
                     GHRPDIIDRSEIKRQRRPGDARNRQAQRLTGRLAGDGGAIGPRSIA"
     gene            2078..2992
                     /locus_tag="VN627_07895"
     CDS             2078..2992
                     /locus_tag="VN627_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03215.1"
                     /translation="MTPIAPGRLGLPQPDDWNEGQRSLILQALPAHLATSRFSDGKTD
                     IVLGGRFADLPLAQAVAGLARRPLKVDPAPGLFGLVVVTTRGWLVVPECREAMSMAID
                     RDAIGPALGLNGWSGTTLIAPPPAGTASAERWADRTLAQRRNEAAARLARCKVRNGDP
                     GPLRLALPNGPGSDALHARLADDLAAVGIALARVPPGVAGDLRLIDAVARYPRGEWYL
                     HQLSCAVLRGPCSEAADAQLAAMEGETDLAKSAQLTNEALGAAGLANFYIPLGTPIRW
                     SLVRDRVGGFLPNSVAFHPLPPLAQTGE"
     gene            3127..3825
                     /locus_tag="VN627_07900"
     CDS             3127..3825
                     /locus_tag="VN627_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXD03216.1"
                     /translation="MRVLLIEDEPTTARAIELMLTAEGFNVYSTDLGEEGLDLGKLYD
                     YDIILLDLNLPDMHGYDVLKKLRVAKVQTPVLILSGIAEMDSKVRSFGFGADDYVTKP
                     FHREELIARIHAVVRRSKGHSQSIIRTGKLAVNLDAKTVEVDSARVHLTGKEYAMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLAHACGGENYIETVWGRG
                     YVLRDPNEQEAEAA"
BASE COUNT          715 a         1302 c         1374 g          801 t
ORIGIN      
        1 gtccgcacgg cattgcgaag cgtcgtcata gccgtgcacg gcggcaaggt tgaaccactt
       61 gtgcgcctcg atgagatcga tggctgcgcc gtggcttccc gtcgaatagg cgaccccgag
      121 atcgaagtag gcgctgagat cgccttgcgc cgcagcggcc atgcagcttg ccaccagcat
      181 atcggcggcg gattctccga ccggttcgaa cgaaaccggc attgtccagc ttgcatccat
      241 ggtgctcgta ccccctactg cggacctgcc cctgtttgct ggtccgacac ggcaaagctg
      301 aacgatcgtg gtcaacaaaa ggttaatgcc agaagggcga tgttgaatta ggcccagcat
      361 catgatttcc gacgtgctgg cactactttg caagacggag ccatcatagc gcttgtctgc
      421 gcccgaaccc ctcagtatag gcccgcacaa cccggaggtc gggctgtctc ggcaagttcg
      481 ccggggccgt cccggcgctg atgatgcctc ggaggcgcaa ggaatttgca tgcccgtttc
      541 gctggctgac gatatcgaca ttctggtcaa ggcaaagcgt tccattgccg gggactacct
      601 gccatccgat gatgagccct acatgggcga ggcacagcag gattacttcc gccgcctcct
      661 ccaggcgtgg aagaaggcga tcctcgattc gtcggccgat actctccagc agttgcagga
      721 tggcccgatt cgcgagccgg acctcaacga tcgcgcgtcg agcgaaacgg attggggtat
      781 cgagctgcgc acgcgcgatc gccagcgcaa gttgatcgcc aagatcgatt cggccctgcg
      841 gcgcatcgaa gagggcgaat acggctattg cgaagtgacc ggcgaaccga tcggcctcgg
      901 ccgtctgatc gcccgcccga tcgccaccat gacggtcgag gctcaggagg cgcacgagcg
      961 ccgcgagaag atttctcgcg acgattgagc cactgcttcg taaacggact tatcgtttac
     1021 gggttgtttg ctttcccgga ctattgccgc gggtgcggtg ttgctgccgg ttcggcacgg
     1081 cgcgcccaat gtaaacccgt gcggaacgat gagcgacagc gacaaccgga atatctcccg
     1141 cgacagcctg ttcatcctgg ctgagctgcg cattgatggg cggggggacg agcatcgggt
     1201 caaggtgcgc aacctctctg cgggaggcat gatggccgaa ggcccggcaa gggtcgcacg
     1261 cggcgaacgg gtcgaggtca acctgcggaa tgtgggctgg gtcgagggtt cggtggcctg
     1321 ggtccaggaa aaccggtttg gcattgcctt cgccgaagat ctcgacccct cggtaatcag
     1381 ggttcctgtc gcggcaagtg ccgaccaaac tccgcgacaa ctcaaaaaca tttatgcgag
     1441 cgctccggcg cccggtccgg cgcgcaagat cttctgaacc ctccggcaat ctaccttgca
     1501 gcgcacgggg cctgcgctta ggccttggcc gatgaggacc ctgcgcgtcg ctcttgtggc
     1561 tcttgcagct gccagtctct cggcttgcgg cataggttcc gcgcccaccg ctgaaattga
     1621 cgtcatcggc gcgcccggcg atcccttcga ggccggggtt cgcctgtcca cagcggggca
     1681 gcttgtccgc gctgccacgg ctgaagggct tgtcgggctt gacgagcagg ggcgggtcat
     1741 cccggcagtg gctgaccgtt ggatcgtaac ggacgatggc ctcagctata tctttcgtct
     1801 acgcgatgga tcgtggcccg atggcgccgc catctccggc caatctgccc gtgaggcgct
     1861 gcgcctggcg attgcgggcg tcgccgggac gtcgctggcg cttgatctcg ctgcgatcga
     1921 tgatgtccgg gcgatggccg gccgtgttct cgaaatccgt ctccgccgtc ccgttcccga
     1981 ccttctgcaa ttgctggccc aaccggaact cggcctcctg cgcgatggtg ccggcggcgg
     2041 gccgatgacc ttgcggcgat ccgggcagca agcggtgttg acgccgatcg cgcctggtcg
     2101 gctgggcttg ccccagccgg acgattggaa cgagggacag cgcagcctca tccttcaggc
     2161 gctgcccgcc catctggcga cgtcccggtt ctcggacggc aagaccgaca tcgtcctagg
     2221 cggacggttc gcggacttgc ccctggccca ggccgttgcc ggtctcgcgc gcaggccctt
     2281 gaaggtcgat cctgcgccag gattgtttgg cctggtggtt gtcacaaccc gcgggtggct
     2341 ggtcgtcccg gagtgccggg aggcgatgtc catggcgatc gaccgggatg cgattggtcc
     2401 cgcgctcggc ctgaacggct ggtcaggtac gaccctgatc gcgccgccgc ccgccggaac
     2461 agcatcagcg gagcgatggg cagaccgcac cctggcccag cgccgtaatg aagcggcggc
     2521 tcggctggcg cgctgcaagg tccgcaacgg cgatcccggg ccgctcaggc tcgccctccc
     2581 caacggcccc ggctccgacg cgctgcatgc ccggctggct gacgacctcg ccgcggtcgg
     2641 catcgccctg gcgcgggtgc cgccgggggt ggctggggac cttagactga tcgatgcagt
     2701 ggcccgctac ccgcgcggtg agtggtatct tcaccaactg agctgcgccg tgttgcgcgg
     2761 gccgtgcagc gaggccgccg acgcgcagct cgcggcgatg gaaggcgaaa cggacctcgc
     2821 aaaatccgcg caattgacca atgaggcact cggcgcggct ggcctggcaa acttttatat
     2881 cccgttgggc acacccatcc gttggtcatt ggttcgggac cgggttggcg gatttctgcc
     2941 gaattcagtg gcttttcatc cacttccgcc gctggctcaa acaggcgagt aagttggtgg
     3001 ctgcgcatcg cctggccgct taaatctaaa ttaacctttc tctttcatag ctcctatggt
     3061 taattgctcg agcactctcg aaacaggagg gggtgttcaa gtttcggggc ataaggggga
     3121 ctacccatgc gagtgctgtt gatcgaggac gagccgacca cggcccgggc catcgagctg
     3181 atgctcacgg ccgagggctt caatgtctac tcgaccgacc ttggcgaaga gggcctggat
     3241 ctgggcaaac tctacgacta cgacatcatc ctgctggacc tcaacctgcc cgacatgcac
     3301 gggtacgatg tcctcaagaa gctgcgcgtc gccaaggtgc agacgccggt gctgatcctt
     3361 tctggcattg ccgaaatgga ttccaaggtc cgttcgttcg gcttcggtgc ggacgactat
     3421 gtgaccaagc cgttccatcg cgaagagctg atcgcccgca tccatgcggt tgtccgccgg
     3481 tcgaagggcc attcgcagtc gatcatccgg accggcaagc tggcggtgaa cctcgatgcc
     3541 aagacggtcg aggtggacag cgcgcgggtg catctgaccg gcaaggaata tgcgatgctc
     3601 gagctgctca gcttgcgtaa gggcacgacg ctgaccaagg aaatgttcct caaccacctc
     3661 tatggcggga tggacgagcc ggaactgaag atcatcgacg tcttcatctg caagctgcgc
     3721 aagaagctgg cccatgcctg cggcggcgag aactacatcg agacggtgtg ggggcgcggc
     3781 tacgtgctgc gcgatcccaa cgagcaggag gccgaggccg cctgaagcag cctgcgccga
     3841 ttcggcaacc ccaggaattc cggcggcccg cttgcccttt ggcacgcggg ccgtctcgcg
     3901 tttcgggtct gtttcacgcg gatgttccac ggacatgtgg ctgagaacac ggcgttagtt
     3961 agttgatatt gtacatgcgc gcgtttgcgt ttctgccgca gggcgtgggt cgcggttcgc
     4021 ggttgccggc tgaggggctg acgatgagaa agactgcggc cgctttatgc cacgggtggg
     4081 ggcctgtagg acaccagcgg gccagggtcc tgaccctagc gattgcggta gttgggcctg
     4141 cgcttctcgt tgaaggcggc gatgccttcg ccgacgtctt cccaggcgct gg
//
LOCUS       DATNBV010000330         7064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_202493,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000330 DATNBV010000000
VERSION     DATNBV010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202493"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2108)
                     /locus_tag="VN627_07905"
     CDS             complement(<1..2108)
                     /locus_tag="VN627_07905"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXD03217.1"
                     /translation="MTAQMLDKTFDPAAIEARWYAHWEGHGLFRPDRPQAQPYTIVNP
                     PPNVTGSLHIGHALDNTLQDILIRYERLRGKDALWVVGTDHAGIATQMVVERQMEAAQ
                     DKRTNYSREAFVDKVWEWKAESGGTITRQLRRLGCSMDWSREQFTMDPHFTRAVVKVF
                     VDLYNEGLIYRDKRLVNWDPKLKTAISDLEVETRETKGGFWHFKYPLADGARRDDGLD
                     YIEVATTRPETMLADMAVAVHPDDPRYRSVIGKEILQPLTGRRFKVVADEHADPELGS
                     GAVKITPGHDFNDFEVGKRAGIKPSEMLNMFDAEARVVQTADGLVPAEFVGLDRFAAR
                     DLVVARMKDAGFLIPHVTKDKEGTEIEADFEPRTIQTPYGDRGGVIIEPWLTDQWYVD
                     AEKLAVAPLEAVRSGAIEIVPKTWEKTFFNWMENIQPWCVSRQLWWGHRIPAWYAEDG
                     EVFVAETEEQARALAGNKSLTRDEDVLDTWFSSALWPFATLGWPDDSELVRRHYPNDV
                     LVSGFDILFFWDARMAMQGLHFMKEVPWKKLYLHGLVRAADGQKMSKSKGNVVDPLGL
                     IDQYGADALRFFMSAMESQGRDVKMDEKRVEGYRNFATKLWNAARFCQSNGIGGSASV
                     AAPQATSAVNKWIIGEVVETVALLDQAMADLRFDAAANTIYHFVWDTFCDWYIELIKP
                     VSSGDERGSFDEETKAVAGWV"
     gene            2231..3337
                     /locus_tag="VN627_07910"
     CDS             2231..3337
                     /locus_tag="VN627_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HXD03218.1"
                     /translation="MRQLAEYTTRDDAGGTTVALAGPLTVSRVGALDQQLRTLGDVAR
                     VDLSGVTAIDTVGAWIAWRLSRGGEAEITGESDEARRLIEAVSGTSTVAEVAPPRPEL
                     LERVPEAVGTLITSMGQGSVGITGFLGQIILALGQTIREPRRLRVRSLVRQAELVGVS
                     ALGIIGLMSFLVGIVIAQQGAVQLQQFGAEVYTVNLTGRLAMRELGVLMTAIMVAGRS
                     GSAFAAQIGTMKLTEEVDAMRTIGVSPVEALVLPRIFAAVLMMPLLGFYAAVLAIIGG
                     AFISDFVLGIPFMTFLMRIQEVVPIRDLWVGMVKAPFFGLIVALCGCYQGMNVRANAE
                     EVGLKTTMAVVQAIFMVIVLDAFFAVFFTKVGWG"
     gene            3367..4209
                     /locus_tag="VN627_07915"
     CDS             3367..4209
                     /locus_tag="VN627_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXD03219.1"
                     /translation="MIAASGDAPEFPIVVEGLVNRFGEHTIHEGLDLTVRKGEILGVV
                     GGSGTGKSVLMRSIIGLQVPDAGSITVLGRSITDARDDEDIDIRSRWGVLFQGGALFS
                     TLTVGENVEVPLKEFYPEIAPALLHEIATYKVMLTGLPEEAAAKYPSELSGGMKKRAG
                     LARALALDPELLFLDEPTAGLDPISAAAFDALTLELQETLGLTVFLITHDLDTLYEIC
                     DRVAVLADGKVIAIGTIPELLATDHPWIQEYFNGPRGRAARDSREMAGARAAGRSGQT
                     GESA"
     gene            4213..5151
                     /locus_tag="VN627_07920"
     CDS             4213..5151
                     /locus_tag="VN627_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXD03220.1"
                     /translation="METRANHVWVGAITLALIALVAAFVLWITRVGEGDQQPYDIFFK
                     QSVDGLAKGAQVTFAGVPAGQIDKIELWPRDPSFVRVRIKVDKKIPVLQGTTATIQGS
                     FTGVSSLLLEGAVKGAPPITEPGPEGAPVIPTKRGGLGALLNNAPLLLERLATLTERL
                     TQLLSDENQRSITHILANTDRMTAHLADASPQVERTLAELQVTLRQASLTLAAFEKTA
                     NSANGLLEGEGKPLAAQLRQTLASAQKAAETLNATVSDARPAARQLSDTTLPAAEAAI
                     RDLRETTRALRTITEKIDDQGAGSVIGGSKLPDYKP"
     gene            5148..5762
                     /locus_tag="VN627_07925"
     CDS             5148..5762
                     /locus_tag="VN627_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HXD03221.1"
                     /translation="MTMTRASSLRLALGAALAPVLAMALAGCVNLGGGKAPPTLFTLT
                     AAAVPPAGETVSGNADSALVVLDPETDRALGVQRVAVTIDASNIAYLKNAMWAERPSR
                     LFRHLLAETIRAKGGRLVFEDAEASASGRVRLAGRLLAFGYDAPSRAALVRFDAVREG
                     AGGAITTRRFEASVPVGKADARLVAPALNGAANAVAAQVADWVG"
     gene            5830..6654
                     /gene="map"
                     /locus_tag="VN627_07930"
     CDS             5830..6654
                     /gene="map"
                     /locus_tag="VN627_07930"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXD03222.1"
                     /translation="MNQYQTVTGDEVQLRDGTIKLHGAAAFEGMRKAGRLAAEILDEL
                     APLVKPGVTTGEIDDMVRRLTMEGGAVPATLGYRGYAHSCCTSINNVICHGIPGDKVL
                     KDGDIVNIDVTPLLDGWHGDSSRMYLVGDVPLKARRLVEVTYECLMIGIEQARPGKRL
                     GDIGAAIQKHAEGNRYGVVREFCGHGLGRLFHDAPEVIHAARAGTGPELKPGMFFTIE
                     PMINLGKPGVKMLDDGWTAVTRDRSLSAQFEHSIGITEDGCEIFTLSPKGLHKPPY"
     gene            complement(6819..>7064)
                     /locus_tag="VN627_07935"
     CDS             complement(6819..>7064)
                     /locus_tag="VN627_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03223.1"
                     /translation="RPTGQTIVWHHPVPKSRGGRDVVPMHPICQQTLIANFTNSELQR
                     HGMDVEGLLLNPSVRKFVDWVANKDPDFTATTAKKQR"
BASE COUNT         1130 a         2277 c         2394 g         1263 t
ORIGIN      
        1 acccagccgg ccacggcctt cgtttcctcg tcgaatgagc ctctctcgtc tccgctcgag
       61 acaggcttga tcagttcgat gtaccagtcg cagaaagtgt cccacacgaa gtggtagatc
      121 gtgttggcgg cggcatcgaa gcgcaggtcg gccatggcct ggtcgagcag ggcgacagtt
      181 tcgacgactt cgccgatgat ccacttgttg accgcgctgg tggcctgcgg cgcggcgacg
      241 gaagccgagc cgccgatccc gttgctctgg cagaagcgcg cggcgttcca caattttgtc
      301 gcgaaattgc ggtaaccttc gacccgtttc tcatccattt tcacatcgcg gccctggctt
      361 tccatggcgg acatgaagaa gcgcagggca tcggcgccat attggtcgat gaggccgagc
      421 gggtcgacca cgttgccctt ggacttggac atcttctggc catcggcggc gcggacgagg
      481 ccgtggagat agagcttctt ccacggcact tccttcatga aatgaaggcc ttgcatggcc
      541 atcctcgcat cccagaagaa caggatgtcg aagccggaga cgagcacgtc gttggggtag
      601 tggcggcgga ccagctcgct gtcgtctggc cagccgagcg tggcgaaggg ccagagggcg
      661 ctggagaacc aggtgtcgag cacgtcctcg tcacgggtga gagatttgtt gcccgcgagg
      721 gcgcgggcct gttcctcggt ttccgcgacg aaaacctcgc cgtcttccgc ataccacgcc
      781 ggaatccggt gaccccacca caattggcgg ctgacgcacc acggctggat gttctccatc
      841 cagttgaaga aggtcttctc ccaggtcttc ggcacgatct caatggcgcc cgaacggacg
      901 gcttccagcg gggcgaccgc gagcttttcg gcgtcgacat accactggtc ggtcagccac
      961 ggctcgatga tcacgccgcc gcggtcgcca tagggcgtct ggatcgtgcg cggctcgaaa
     1021 tcggcttcga tttccgtgcc ttccttgtcc ttcgtgacat gcggaatgag gaatccggcg
     1081 tccttcatcc gcgcgacgac aagatcgcgg gcggcaaagc ggtcaagccc gacgaattcg
     1141 gccgggacca ggccgtcggc ggtctgcacc acgcgggctt cggcatcgaa catgttgagc
     1201 atttccgaag gcttgatgcc ggctctcttg cccacttcga agtcgttgaa atcatgcccc
     1261 ggggtgatct tcaccgcacc cgagccgagt tcgggatcgg cgtgttcatc cgccacgacc
     1321 ttgaagcgcc ggcctgtcag cggctggagg atctccttgc cgatgacgct ccggtaacgc
     1381 ggatcgtccg ggtgaacggc gacggccatg tcggccagca tcgtttcggg ccgggtggtc
     1441 gccacctcga tatagtcgag cccgtcgtcg cggcgcgcgc catcggccag cggatacttg
     1501 aagtgccaga agccgccctt ggtctcgcgg gtttcgacct caaggtcgga gatcgcggtc
     1561 ttgagcttcg ggtcccagtt caccagccgc ttgtcgcggt aaatgaggcc ctcgttgtag
     1621 agatcgacga agaccttcac caccgcgcgg gtgaagtgcg ggtccatggt gaactgctcg
     1681 cggctccagt ccatcgagca accgaggcgc cgcagctggc gggtaatggt gccgccgctc
     1741 tcggccttcc attcccagac cttgtcgacg aaggcctcgc ggctgtagtt cgtgcgcttg
     1801 tcctgagcgg cctccatctg ccgttcgacc accatctggg tggcgatgcc cgcatggtcg
     1861 gtgccgacca cccacagcgc gtctttccca cgcaaccgtt cgtagcggat cagaatgtcc
     1921 tgaagtgtgt tatccagcgc atggccgatg tgcagcgatc cagtcacgtt cggcggaggg
     1981 ttcacgatgg tatagggctg cgcctgcggc cgatccgggc ggaacaggcc atggccttcc
     2041 caatgggcat accacctggc ttcgatggcg gcggggtcga aggtcttgtc gagcatctgg
     2101 gcagtcatag cggggcgctt tgccagtgcc cccgcgcggg tgcaacccat aggacggagc
     2161 gagggcaggt tgcggcgaac ggttcaccgc ccttgtcgcc cccggcattt cccggcatga
     2221 cccctctggc atgaggcaac tggccgaata caccacccgc gacgacgcag gcggcaccac
     2281 ggtggctctc gcggggcccc tgacggtgag ccgcgtgggg gcgctcgacc agcaactgcg
     2341 cacgctgggc gatgttgcgc gggtggacct ttcgggcgtc accgccatcg atactgtcgg
     2401 cgcgtggatc gcctggcgcc tgtcgcgcgg gggcgaggcc gagatcaccg gggaaagcga
     2461 cgaggcccgc cgcctgatcg aggcggtgag cggcacctcg acggtggccg aagttgctcc
     2521 gccgcgtccg gaactgctgg agcgcgtgcc agaggccgtg gggacgctga tcacctcgat
     2581 gggccagggc tcggtcggga tcaccggctt tcttggccag atcatcctcg cgctgggaca
     2641 gaccatccgt gagcctcgcc gcctgcgcgt gcgctcgctg gttcgccagg ccgagctggt
     2701 gggcgtttcg gcgctgggca tcatcgggtt gatgagcttc ctggtcggca tcgtcatcgc
     2761 ccagcagggc gcggtccagc tccagcagtt cggggccgag gtctatacgg tcaacctgac
     2821 cggccgcctc gccatgcgcg aactgggcgt gctgatgacc gcgatcatgg tcgccggccg
     2881 ctcgggctcg gcgtttgccg cccagattgg cacgatgaag ctgaccgagg aggtcgacgc
     2941 catgcggacc atcggcgtct ctccggtcga ggctctggtc ctgccgcgga tcttcgccgc
     3001 cgtgctgatg atgccgctgt tgggcttcta tgcggcggtg ctggcgatca tcggcggcgc
     3061 gttcatttcc gatttcgtcc tgggcattcc gttcatgact tttctcatgc gcatccagga
     3121 agtcgtcccc atccgcgacc tgtgggtcgg catggtcaag gcgccgttct tcgggctgat
     3181 tgtcgcgctt tgcggctgct accagggcat gaacgtgcgc gccaatgccg aggaagtggg
     3241 gctgaagacg accatggccg tggtccaggc gatcttcatg gtcatcgtgc tcgacgcctt
     3301 cttcgcggtg ttcttcacca aggtgggctg gggatgacgg acaatgccgc ccccctcgat
     3361 tcgaccatga tcgccgcgtc cggcgacgcg cccgagttcc cgatcgtggt cgaaggcctc
     3421 gtcaaccggt ttggcgagca caccatccac gaagggctcg atctcacggt gcgcaagggc
     3481 gagatccttg gtgtggtcgg cgggtcgggg accggcaagt cggtgctgat gcgctcgatc
     3541 atcgggctgc aggtgcccga tgccggatcg attacagtgc ttggccgttc gatcacggat
     3601 gcccgggatg acgaggacat cgacatccgt tcgcgctggg gcgtcctgtt ccagggcggc
     3661 gcgctgtttt ccactctcac cgtgggcgag aatgtcgagg ttccgctgaa ggagttctac
     3721 cccgagatcg cgcccgcgct gcttcacgag atcgccacct acaaggtcat gctcacgggc
     3781 ctccccgaag aggcggcggc aaaatatccg tccgagcttt caggcggcat gaagaaacgc
     3841 gccggccttg cccgggcgct ggcgctcgat cccgaattgc tgtttctcga tgagcccacc
     3901 gccggcctcg atccgatcag cgccgccgcc ttcgacgcgc tgacgctgga actgcaggag
     3961 acgcttggcc tcaccgtgtt cctgatcacc cacgatctcg acacgctcta cgagatctgt
     4021 gaccgggtcg ccgtgctggc ggatggcaag gtgatcgcca tcggcaccat accggaactg
     4081 cttgccacgg atcacccgtg gatacaggaa tacttcaacg ggcccagggg cagggccgcg
     4141 cgcgacagtc gggaaatggc cggggcgaga gccgcaggcc gttccgggca aacaggggaa
     4201 agtgcctgag ttatggaaac gcgggccaat cacgtctggg tcggggcgat cacgctggcg
     4261 ctgatcgcgc tggtggccgc cttcgtcctg tggatcaccc gcgtgggcga gggcgaccag
     4321 caaccttacg atatcttctt caagcaatcg gtcgacggcc tcgccaaggg cgcgcaggtg
     4381 accttcgccg gcgttcccgc ggggcagatc gacaagatcg agctgtggcc gcgcgatccc
     4441 agtttcgtgc gggtgcggat caaggtcgac aagaagatac ccgtcctcca gggcaccacg
     4501 gccaccatcc agggcagctt cacgggcgta tcctcgctcc tgcttgaagg cgcggtgaag
     4561 ggcgctccgc cgatcaccga gccggggcct gaaggcgcgc cagtgatccc gaccaagcgc
     4621 ggcgggcttg gcgccctgct caacaatgcg ccgcttttgc tcgagcggct ggcgacgctg
     4681 accgagcgcc tgacgcagct gctgtcggac gagaaccagc gctcgatcac ccatatcctc
     4741 gccaataccg accggatgac cgcgcatctc gccgatgcct cgccgcaggt ggagcgcacc
     4801 ctggccgagc tgcaggttac cctgcgccag gcgagcctga ccttggccgc cttcgagaag
     4861 accgccaaca gtgccaatgg tctgctggag ggtgagggca agccgctcgc cgcccagctg
     4921 cgccagacgc tggcttcggc gcagaaggcg gcggaaacgc tcaacgcgac ggtgagcgac
     4981 gcccggcccg ccgcgcggca gttgtcggac accacgctgc ccgccgcaga ggccgcgatc
     5041 cgcgacctgc gcgaaactac gcgggccctg cggaccatca ccgagaagat cgacgaccaa
     5101 ggggccggtt ccgtgatcgg cgggtcgaag ctgccggatt acaagccatg acgatgaccc
     5161 gtgcttcctc tcttcggctg gcgctgggcg ccgcgcttgc ccccgtcctg gccatggcgc
     5221 tggcgggctg cgtcaacctt ggcggcggca aggcgccgcc gaccctgttc accctgaccg
     5281 ccgccgcggt gccgccggcg ggcgaaaccg tcagcggcaa tgccgacagc gcgctggtcg
     5341 tgctcgatcc ggaaaccgac cgggcgctcg gcgtgcagcg ggtggcggtg accatcgatg
     5401 cttccaatat cgcctatctg aagaacgcga tgtgggccga gcgcccgtcg aggctgttcc
     5461 gccatctcct tgccgagacg atccgcgcca agggcggccg gctggtgttc gaggacgccg
     5521 aggccagtgc cagcggccgg gtgcggctcg ccggcaggtt gcttgccttc ggctatgacg
     5581 cgccgagccg cgccgctctg gtgcgcttcg atgcagtgcg cgagggcgcc ggaggggcga
     5641 tcacgacccg ccggttcgag gcgagcgtac cggtgggcaa ggccgacgcc cggctggtgg
     5701 ctccggcgct caacggcgcg gccaatgccg tcgcggcgca ggtggccgac tgggtggggt
     5761 gaagctgttg tgccggcgcg tggcgatggc gggtcgcaaa agcctgtcat catcgctatg
     5821 tgagcgcgca tgaatcagta ccagacagtc accggcgacg aagtccagct gcgcgacggc
     5881 acgatcaagc tccacggcgc cgcagccttc gagggcatgc gcaaggccgg ccgccttgcc
     5941 gccgagatcc ttgacgagct tgcccccttg gtgaagcccg gcgtcaccac cggcgagatc
     6001 gacgacatgg ttcgccggct caccatggag ggcggcgcgg tgccggccac gcttggctat
     6061 cgcggctatg cccattcgtg ctgcacttcg atcaacaacg tgatctgcca cggtattccc
     6121 ggcgacaagg tcctgaagga cggggacatc gtcaacatcg acgtcacccc gctgctcgat
     6181 ggctggcatg gcgattcgag ccggatgtat ctcgtcggcg acgtgccgct gaaggcgcgg
     6241 cggctggtcg aggtgaccta cgagtgcctg atgatcggga tcgagcaggc gcggcccggc
     6301 aagcggctgg gcgatatcgg cgcggcgatc cagaagcacg ccgagggcaa ccgctacggc
     6361 gtggtgcgcg agttctgcgg gcatgggctg gggcggctgt tccacgatgc cccggaagtg
     6421 atccatgctg cgcgggcggg gaccgggccg gagctgaagc cgggaatgtt cttcacaatc
     6481 gagccgatga tcaacctcgg caagcccggg gtgaagatgc ttgatgacgg gtggacggcg
     6541 gtgacgcggg accgttcgct gtcggcgcag ttcgagcatt cgatcgggat taccgaggat
     6601 ggctgcgaga tcttcaccct ctcgcccaag gggctgcaca agccgcctta ttgaggggaa
     6661 gggggaactg ccgtcgtccc gggcttgacc cgggacccct gtggaacctt caggcgaaac
     6721 ctgtcgggca ggcacgcgca tagctgcgcg atggccagcg gtcccgggtc aagcccggga
     6781 cggcgattgc tcgcccttca ccccactgcg cgctccgttc accgctgctt cttcgccgtt
     6841 gtcgcggtga agtccgggtc cttgtttgct acccagtcga cgaacttgcg cacgctgggg
     6901 ttcagcagga gcccttccac atccatgccg tggcgctgca attcggaatt ggtgaaattg
     6961 gcgatcagag tttgctggca gatcgggtgc atcggcacga cgtcgcgccc gccgcggctc
     7021 ttggggaccg ggtggtgcca gacgatggtc tgcccggtcg gccg
//
LOCUS       DATNBV010000331         3893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1779009,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000331 DATNBV010000000
VERSION     DATNBV010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779009"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VN627_07940"
     CDS             <1..1002
                     /locus_tag="VN627_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-related domain-containing protein"
                     /protein_id="HXD03224.1"
                     /translation="LKRLGSPNFTHLPINAPRCPMAHFQQDGHMAMRNPKGRANYEPN
                     SWGPLMGGPREDPERGIRSFAAEGDGPKQRLRPESFADHYSQARQFFVSQTPIEQKHI
                     GDALVFELSKVERPDIRVRVVSHLINIDPGLAQTVASGLAVALPEPAQAAMPTRQDLA
                     PSPALSIIANGPGGFAGRKLGILVADGTDAVLYDALVEAVTAAGGVYEVIAPNIGGVV
                     LSDGSALAAKHKIDGGPSVLFDAVAVIPSAEGAQLLAGDGPTRDFVSDAFVHCKFIGL
                     SDGGAALMAGAGLGELLDEGCVALQERECEAFIAKCAPLRYWPRELDVDADTASVSS"
     gene            1137..1901
                     /locus_tag="VN627_07945"
     CDS             1137..1901
                     /locus_tag="VN627_07945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD03225.1"
                     /translation="MTTQPGLADAHTEMPSLQGRRAVITGGTTGIGRAIAVLLASEGV
                     DVFICGRDPRHLADALERIREVGQGDGVALDLAEDGAPQRFFEAAERVTGPPDIAVIN
                     AAVPADALAETSPKATAYQVAVDFGAYLATAQLAATRMNGGADIILIGSMSAVSQGPG
                     SSIYVAAKSGIEGLAPSLRKELAEKDIKVGLIEPGFTGADFQYPEFPPEKQRELIEEN
                     KMLRAEDIAVAVHFMLRQPRRCAVSLMRVETRLNHP"
     gene            1928..2239
                     /locus_tag="VN627_07950"
     CDS             1928..2239
                     /locus_tag="VN627_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03226.1"
                     /translation="MGEHLIQILLPLERRGGQAQPRERYGATRDELVERCGGVTIYAR
                     APAEGLWDDGGSVVEDLVVVFEAMDVRFSVAWWAAYKRRLAERFEQEEVLVRAIPVSR
                     I"
     gene            2445..3221
                     /gene="xth"
                     /locus_tag="VN627_07955"
     CDS             2445..3221
                     /gene="xth"
                     /locus_tag="VN627_07955"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXD03227.1"
                     /translation="MRIATFNVNGVNGRLPVLLRWLAETGPDVVCLQELKASDERFPE
                     GSIGDLGYDVVWRGQKSWNGVAILSRCGEIHETCRGLPGDPADAQSRYLEAAVNGVLV
                     AALYLPNGNPRPGPKFDYKLAWMERLINRAAELIAGNLPVVLAGDFNVIPTERDVYKP
                     ERWLDDALFVPEVRHAFDRLLAQGWTDTLRQRHPDETIYTFWDYFRNAWARDAGLRID
                     HLLVSPVLLDRVRDAQVDSFVRGWEKASDHAPVWLELDPG"
     gene            3218..3403
                     /locus_tag="VN627_07960"
     CDS             3218..3403
                     /locus_tag="VN627_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03228.1"
                     /translation="MSARLARLAALPLFKAGGVLARLRGPMPFQLGAFCMVAGLQFAM
                     LARELGLLGLKAGQFLR"
BASE COUNT          638 a         1237 c         1321 g          697 t
ORIGIN      
        1 ctcaagcggc tcggcagccc caatttcacg cacctgccga tcaatgcgcc gcgctgcccg
       61 atggcccact tccagcaaga cgggcacatg gcgatgcgca atcccaaggg ccgggcgaac
      121 tacgagccga actcctgggg cccgctaatg ggtgggccgc gcgaggaccc cgagcgaggt
      181 atacgcagct tcgccgcgga aggtgatgga cccaagcagc ggctgcggcc agagagcttc
      241 gccgatcact acagccaggc gcggcagttc ttcgtcagcc agacaccgat cgagcagaag
      301 cacatcggcg acgccttggt cttcgagttg agcaaggtgg aacggccgga catccgcgtc
      361 cgcgtggtat cgcacctgat caacatcgat cccggtctcg cgcagacggt cgccagcggg
      421 ctcgcggtcg ccttgccgga accggcgcag gcggcaatgc cgacgcggca ggacctggca
      481 ccttcgccgg cactcagcat cattgccaac ggcccgggag ggtttgccgg gcgcaagctc
      541 ggcatcctgg tggccgacgg caccgatgcg gtgctctatg atgcactggt cgaggcggtg
      601 accgcggctg gcggcgtcta cgaggtgatc gccccgaaca ttggaggcgt ggtcctctcg
      661 gatggatccg ccctggccgc caagcacaag atcgatggtg gcccctcggt gctgttcgat
      721 gcagtggcag ttattccctc ggccgagggc gcgcagttgc tggccgggga tgggccgacg
      781 cgggacttcg tcagcgacgc cttcgtccac tgcaagttca tcggcctctc cgatggcggc
      841 gcggccttga tggctggggc cggccttggc gagttgctcg atgagggttg cgtcgcattg
      901 caggaacgcg agtgtgaggc cttcatcgcc aagtgcgcgc cgctgcgcta ctggccgcgc
      961 gagctcgacg tggatgccga tacagcgagc gtttctagct agagtacaaa cggtcggggg
     1021 tcgcggcgcg gcctccgctt gcccgtgaac ggcgggaacg ggatgttccg gggacgtggg
     1081 aacgagtgcg gggcctgagt gctgagtcac ctgaccagca aaggagcaaa gcactcatga
     1141 cgacgcaacc cggacttgcc gacgcccaca ctgagatgcc gtcgctgcag ggccgacgcg
     1201 cagtgatcac gggtggcaca accgggatcg gacgggcaat cgctgtgctc ctggcgagcg
     1261 aaggcgtcga cgtgttcatt tgcgggcgcg acccgcgaca cctcgccgat gcgctcgaac
     1321 gcatcaggga agtgggccaa ggcgacggcg tggcgctcga cctggccgag gacggtgccc
     1381 cgcaacgttt cttcgaggcg gcagagcgcg tgacgggtcc tcccgatata gcggtgatca
     1441 atgccgcggt tccggcggat gcccttgccg agacgtctcc taaggccacg gcctaccagg
     1501 ttgccgtaga tttcggcgcc tatctcgcaa cggctcagct tgccgccacc cgcatgaatg
     1561 gtggggcaga cattatcctc atcgggtcga tgagcgcagt gtctcaggga cctggctctt
     1621 ccatctatgt tgcggcgaag tcggggatcg aaggcctggc gccgtcgctt cgcaaggaac
     1681 tcgcagagaa ggacatcaag gtcggattga tcgaacctgg attcaccgga gccgacttcc
     1741 agtatccgga gttcccgccg gagaagcaac gcgaactgat tgaggagaac aagatgcttc
     1801 gcgccgagga catcgccgtc gccgtgcact tcatgttgcg ccagccgcgg cggtgcgccg
     1861 tgtccctgat gcgggtcgaa acgcggctca atcacccgta aggcgcgggt cacaggcact
     1921 caggctcatg ggcgagcatc tcatccagat cctcttgccg ctggaacgcc gcggtggcca
     1981 agcacaaccg cgagagcgat acggcgcgac ccgggatgaa ctggtcgagc gctgcggggg
     2041 cgtcacgatt tacgcccggg cgccagccga gggcctctgg gatgatggcg gatcggttgt
     2101 agaggatctg gtcgtcgttt tcgaagcgat ggatgtgcgc ttctcagtcg cgtggtgggc
     2161 agcctacaaa cgccgcctcg ctgagcgctt cgagcaggaa gaagtgctgg ttcgcgctat
     2221 cccggtgagc cgaatctgag catgccttgg tgcatcatcc gccatggtcg tcgtgctcat
     2281 ccccgcccga tgggcagact ggctttacct ggcccggccg gaggccaagt tgctggcccc
     2341 gctccccccg gatctctcgt gaccaccctg gtccgccggg gagcggagcc agtgcctagg
     2401 gacttacagg gcctcgccag ccgttttgcg gtcgagagcg ggctgtgagg attgccacct
     2461 tcaacgtgaa cggggtcaac ggcagattgc cggtgctcct ccgctggctc gccgagaccg
     2521 ggcctgacgt cgtctgcctg caggaattga aggccagcga cgagcgcttc cctgagggat
     2581 cgattggcga tctcggctac gacgtggtgt ggcgtgggca gaaaagctgg aacggggtgg
     2641 ccatcctcag ccggtgcgga gagattcacg agacctgccg gggcttgccc ggcgatcccg
     2701 cggacgcgca gagccgttat ctggaagccg ccgtcaacgg cgtcctggtc gcggcgctct
     2761 acctgcccaa tggcaatcct cggcccgggc ccaagttcga ttacaagctc gcctggatgg
     2821 aacggctcat caatcgcgca gccgaactca tcgccggcaa tttgccggtg gtgctggccg
     2881 gcgacttcaa cgtgatcccc accgaacgcg acgtctacaa gcccgaacgt tggctagacg
     2941 atgcgctgtt cgtgcccgag gtgcggcacg cctttgatcg gttgctggcc caggggtgga
     3001 cggacacgct gcgccagcgc catccggatg agacgatcta taccttctgg gactatttcc
     3061 ggaatgcctg ggcccgcgat gccgggttgc ggatcgacca cctgctggtg agcccggtgc
     3121 tcctggatcg ggtcagggat gcgcaggtcg acagtttcgt gcggggctgg gagaaggcca
     3181 gcgaccatgc ccctgtctgg ctcgagcttg atcctggatg agcgctcgcc ttgcccgttt
     3241 agctgctctg ccactcttca aggcgggcgg cgtacttgcg cgcctccgtg gaccgatgcc
     3301 gttccagctt ggcgcgttct gcatggtggc gggcctgcaa ttcgctatgt tggcgcgcga
     3361 gctcggcttg cttggcctca aggctggcca gttcctccgc tgaccggtgt tcgaaggctc
     3421 ttagcgtcgc ctctgccgcg gccagcgcag agcggcttgg ctccgggcgc ctgcgcttcg
     3481 cgggagctgc cgttgccctg cctggttccg gtttattcgg cagggcgagg gctccccgcg
     3541 ccttgcggat gacctcgccc ggtcgggcaa gaacctctgc aatcagcgcc cgctctgtca
     3601 cctcttcggc cgcctccgag gcaaacagat ccttggcgtt gcctcacgcg ctcagcgccg
     3661 cctttcggct gggcgcggcg acataggcgt catggaaccc gattgtcgtc cggaagactt
     3721 tgagtcttag aggcatgttc ggagaaccgg cgaagagcag gcacgatcca gagcgtggcg
     3781 cgcggcggaa cgcaagccgg tccatgcggg ttttctgcgc gcccttcagc attagccgtg
     3841 aggtccttgt ccatgtcccc agcgcagccg caatccgccg tcagcccgca ggc
//
LOCUS       DATNBV010000332         2680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4021590,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000332 DATNBV010000000
VERSION     DATNBV010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4021590"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1049)
                     /locus_tag="VN627_07965"
     CDS             complement(111..1049)
                     /locus_tag="VN627_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HXD03229.1"
                     /translation="MAGKHFIRSGALAALAAGVAMAALPSAASAQDQDREHGRHGGWQ
                     QGGGNGGGSGGERGNGGGWQARSQVQPQVQAQPQVQPAPRVDRGDTPGRGWGSARTEG
                     QRYGRDWSNGGVVNRPDAASRNRAAEQAQQAPQQRTYADAWRRNAEQQQIQRQRDGQR
                     TYADAWRNRSYTPQQYDGRQQAWGGQNWDRNRTGDRRWDGDRRWSGDRSGSWNRDWRR
                     DNRYSWQSYRNQHRDVFRIGRYYSPYNNWSYRRLGVGFFLQPLFFSQDYWIDDPWMYR
                     LPEAYGPYRWVRYYDDALLVDIYSGEVVDVIYDFFW"
     gene            complement(1178..1753)
                     /locus_tag="VN627_07970"
     CDS             complement(1178..1753)
                     /locus_tag="VN627_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXD03230.1"
                     /translation="MNILHLDSSITGENSASRVISAAVVRSLSAADPAARVTYRDLVA
                     EPLGHLTLAELGSPAARAELDAFQKADVIVIGAGMYNLSLPTQLKAWIDRIMVAGETF
                     RYTETGPVGLAGGKRVIVALARGGIYSEGSPARAVEHAERYLRDVLAFIGIADPEFII
                     AEGIAMGEEARNAALGSAVDQARGLNSLVAA"
     gene            1884..2243
                     /locus_tag="VN627_07975"
     CDS             1884..2243
                     /locus_tag="VN627_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXD03231.1"
                     /translation="MANPACRAVSELLGRVGDKWTVLVVKELQQHRCRFNALKRQVGG
                     ISQQMLARTLRMLERDGMVARIVHPTVPPQVEYELTELGRSLAVPVLALADWSFAHLD
                     QIEAHRAAYDAALDEAA"
     gene            2288..>2680
                     /locus_tag="VN627_07980"
     CDS             2288..>2680
                     /locus_tag="VN627_07980"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXD03232.1"
                     /translation="MTEHIPLTTTPLPVRLRVEDFFRLDEAGAFEGYGKTELIRGEVF
                     YMNAQHRPHARMKSRLHIELAQAMAGLGSELDVIVEATVAMPPHSAPEPDLVITSEPE
                     GEGPVPLASVRLIVEIADTTKAGDLGDKA"
BASE COUNT          456 a          872 c          890 g          462 t
ORIGIN      
        1 ccgatttggt cctgggcgac gccagcccgt gaaaaaggcc cccgtccggg tgggacgggg
       61 gccggttcgc cggtccagtc accggcatcc tgataggtaa ggggcgaaac ctaccagaag
      121 aagtcgtaga tcacgtcgac cacttccccg ctgtagatgt cgaccagcag ggcatcgtca
      181 tagtagcgga cccagcgata gggaccgtag gcctcgggca ggcgatacat ccacggatcg
      241 tcgatccagt agtcctggct gaagaacagc ggctggagga agaacccgac gccaaggcgg
      301 cggtacgacc agttgttgta tggcgaataa tatcggccga tgcggaacac atcgcgatgc
      361 tggttgcggt aggactgcca cgaatagcgg ttgtcacgcc gccagtcgcg gttccagcta
      421 ccgctgcgat cgcccgacca gcgccgatca ccgtcccacc gccggtcacc cgtccggttg
      481 cggtcccagt tctggccgcc ccacgcttgc tggcgcccgt cgtactgttg cggggtatag
      541 cttcgattgc gccaggcatc ggcataggtg cgctgcccat cgcgttggcg ctggatctgc
      601 tgctgctcgg cattgcggcg ccaggcatcg gcataggtgc gctgctgcgg cgcctgctgg
      661 gcctgttcgg cagcccggtt gcgcgaggcc gcatcgggcc ggttgaccac gccgccattg
      721 ctccagtcgc gcccataacg ctggccctcg gtgcgagccg aaccccagcc acgaccgggc
      781 gtatcgccgc gatcaacgcg aggggcgggc tggacctgcg gctgagcctg gacctgcggc
      841 tgaacctgcg accgggcctg ccagccgccg ccattgccgc gttcgccgcc gctgccgcca
      901 ccattgccgc caccctgctg ccagccgcca tggcggccat gctcgcggtc ctggtcctgg
      961 gcagaggccg ccgacggcag cgccgccatg gcgacgcccg cggccagagc cgccagtgcg
     1021 cccgatctga tgaaatgctt accggccatg gccgtctctc catttctcgc gccgcaccat
     1081 cggcggcgac ctatgcatag agagatacgc ccggcgcgct gtcctgtttc tgaaccgcgc
     1141 gcaggggtat tcgttcaggt ttgtcgctat tttcctttca ggctgcgaca agtgagttca
     1201 ggcctcttgc ctgatccacg gcgctgccga gagcagcatt gcgcgcttct tcgcccatgg
     1261 cgatcccctc ggcgatgatg aactccgggt cggcaatgcc gatgaaggcc agcacgtcgc
     1321 gcaggtaccg ctcggcatgt tcgaccgctc tggccggcga accttccgaa tagatgccgc
     1381 cgcgggcaag ggcgacgatc acccgcttgc cgccggccag gccgaccgga cccgtctcgg
     1441 tgtagcggaa ggtctcgccg gcgaccatga tgcggtcgat ccaggccttg agctgtgtgg
     1501 gcagcgagag gttgtacatg cccgcgccga tcacgatcac gtccgccttt tggaaagcgt
     1561 cgagctcggc gcgcgctgcg ggcgagccca gttccgccag ggtgagatgg cccaagggtt
     1621 cggcgacgag atcgcgatag gtcacgcgcg cggccgggtc ggcggcgctg aggctgcgga
     1681 ccacggcggc ggagatgacg cggctcgccg agttttcgcc ggtgatcgag gagtcgaggt
     1741 ggagaatgtt catcgtggtg ctccggtggt caacaatctt gaccagcacc agataaatga
     1801 ccatcttgag cctgcaagaa cgcaaatttt tcagaccagg tcacacgcga atgactagct
     1861 cagcccagcc gaaccctcac gccatggcca atcccgcgtg ccgcgcggtc agcgaactgc
     1921 tgggccgggt gggtgacaag tggaccgtgc tggtcgtcaa ggaactccag cagcaccgct
     1981 gccggttcaa tgcgctcaag cgccaggtcg gcggtatctc ccagcagatg ctggcccgca
     2041 ccctgcgcat gctggagcgc gacggcatgg tggcgcggat cgtccatccg acggtgccgc
     2101 cgcaggtgga atatgaactg accgagcttg gccgctcgct tgccgtgccg gtgctggcgc
     2161 tggctgactg gagcttcgcc cacctggacc agatcgaagc gcaccgggcg gcctatgatg
     2221 cggcgttaga cgaggcggcc tagaggggtg gtttgaccca cgtcgcttcc atgatactct
     2281 gcgctgcgtg accgaacata ttcccttgac cacgacgccg ttgcccgtgc gcctgcgggt
     2341 ggaggacttc ttccggctgg acgaagcggg ggcgtttgag ggctacggca agaccgagct
     2401 gatccggggg gaagtcttct acatgaacgc gcagcatagg ccgcatgcgc ggatgaagag
     2461 tcgcctgcac atcgaactcg cgcaagcgat ggccggctta ggctccgagc tcgatgtgat
     2521 cgtcgaagct accgtagcca tgccgccgca cagtgcgccc gaacctgatc tggtaattac
     2581 cagcgaaccg gagggggaag ggccggtccc gcttgcatcg gtacggctta tcgtggagat
     2641 cgccgacacc acgaaagccg gtgatctcgg cgacaaagcc
//
LOCUS       DATNBV010000333         3127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3395861,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000333 DATNBV010000000
VERSION     DATNBV010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3395861"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..2124
                     /locus_tag="VN627_07985"
                     /pseudo
     CDS             34..2124
                     /locus_tag="VN627_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
     gene            complement(2121..2444)
                     /locus_tag="VN627_07990"
     CDS             complement(2121..2444)
                     /locus_tag="VN627_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HXD03233.1"
                     /translation="MDEQVSCTARLWRWTGGNGGTWHFLSLGGEPAEALAATALMRRL
                     EGLGRGFGSLRVRATIGDTTWQTSVFPQKSEDGSAEWILPVKAAVRKAEDLAEGDETA
                     LTLRF"
     gene            2520..2610
                     /locus_tag="VN627_07995"
     tRNA            2520..2610
                     /locus_tag="VN627_07995"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2554..2556,aa:Ser,seq:gga)
BASE COUNT          564 a         1017 c         1045 g          501 t
ORIGIN      
        1 ccttggcggc ccgtgtcccc catctaggca gcaatgctgc gccagtacga actcgttgaa
       61 cgcgtcaaag cctatgatcc ggaggcggac gaggccgcgc tgaaccgcgc ctatgtctac
      121 accgtccaga agcacggcac ccagaagcgg gccagcggcg atccctattt ctcgcacccg
      181 gtggaagtgg ccggcctgat gaccgagctc aagctcgacc aggacacgat catcaccgcc
      241 ctgctccacg acacggtgga ggacactctc gccacggtcg acgaggtgga gcgcctgttc
      301 gggccggatg tggcccggct ggtcgatggc gtcaccaagc tctccaagat cgaggcgatg
      361 accgagaacg agcgggcggc ggaaaacctg cgcaagttcc tcctggcgat gagcgaggat
      421 ctgcgggtcc tgctggtgaa gctcgccgac cggctgcaca acatgcgcac gctccacttc
      481 atcaagagcg aggacaagcg ccgccgcatc gcccgcgaga cgatggagat ctatgccccg
      541 ctggccgagc gggtgggcat gtacgaatac atgcgcgaga tgcagttgct ggctttcgag
      601 cagctagagc ccgaagccta cgcgacgatc accgggcggc tggcccagat ccgcagttcc
      661 gagggcggcc aggtcggcgc gatcgcgctc tcgatcaagc aggcgctggc cgaggcgggg
      721 ctcaaggtgg aggtctcggg acgggagaag cacccctatt cgatctggaa gaagatggcc
      781 gagcgccatg tctcgttcga gcagatcacc gacatcatgg ccttccgcgt gatcgtcgac
      841 gaggtgggag actgctacaa ggccctgggc gtgctgcatc aggcgtggca gatgatcccg
      901 ggccggttca aggactatgt ctcgacgccc aagtcgaacg gctaccggtc gctccatacc
      961 tcgctgatct ttgagaattc gatgcgcatg gaggtgcaga tccgcacccg tgagatgcac
     1021 cttaccaacg aatatggcct cgccgcccac tggtcctaca agcagggcgg ggtgcgcccc
     1081 gatggcgcgg tgggctggct gcgcgacctg atcgagatcg tcgatgccag ccacgatgcc
     1141 gaggaattgc tcgagcatac gcgccttgcc atctaccagg accggatctt tgccttcacc
     1201 cccaagggcg cgctgcacca gctgcccaag ggcgcgacgc cggtggacct ggcctattcg
     1261 gtccacaccg acctcggctc gatggccgtg ggcgccaaga tcaatggccg ccacatgccg
     1321 ctgcgcaccg tgctcaacaa cggcgacgtg gtggagatca tcaagagcaa gaccagttcg
     1381 ccgcagctgt cgtggctgtc gttcgtggtc accggcaagg cgcgggcggc catccgccgg
     1441 gcggtgcgcg ccaaggagcg ggcggagatc gccgcgatcg gccgcaagct gttcgaggag
     1501 atcgccagtc gcctgccgac caaggtcggc aagaaggcga tccgcgaggc cgtgggtcgg
     1561 ctggggcttg aggacgagga agacctgatg gtcgccatcg gcacggccaa gctgtccgac
     1621 cggcaggtga tggaggcgct cgtccccggc tcggccgcca atctcccggc ggaggactag
     1681 cccaagcagg agcgggcgat ctcgatccgc gggctcaccc ccggagtggc cttccggctg
     1741 gccgaatgct gccacccggt gcctggcgat cgcatcgtcg gcctcaagcg ccccggcgag
     1801 ggggtcgagg tccacgcgat cgactgcatg agcctggccg acggcatcga tgccgactgg
     1861 atcgacctgt cctggggcga gcgcagcacc ggcgccatcg gccagatccg cgccgtcctg
     1921 ttcaaccggc cgggcacgct ggccgagatg accgggatct tcgccaatgc ccacgccaac
     1981 atcgtgcgcc tggagatgac cagccgcgac gacatgttcg ggacctacga agttgacctg
     2041 gaggtcaccg acctggcgca cctcacccgc atcgtctcgg ccttgcgggc gagcgaggcc
     2101 gtggccagcg cggagcggat ctagaagcgc agggtgaggg cggtctcatc gccttcagcg
     2161 aggtcttccg ccttgcgcac ggcggccttg acggggagga tccactcggc cgagccatct
     2221 tcggatttct gcgggaaaac cgaagtctgc caagtggtat cgccgatcgt cgcgcgcacc
     2281 cgcagcgacc cgaacccgcg gccgagcccc tccagccgcc gcatcagcgc ggtcgcggcc
     2341 agcgcctccg cgggctcgcc gccgaggctg agaaaatgcc acgtcccgcc gttgccgccg
     2401 gtccagcgcc acagccgcgc ggtgcaggag acctgttcgt ccatgcgcgc gaccctgcca
     2461 cgacagagcg ccttgcgaaa cccgtgccgg acggctaggg cgcgccttcc agtgcatgcg
     2521 gagcggtggc cgagtggtcg aaggcgctcg cctggaaagt gagtatacgt caaaagcgta
     2581 tcgagggttc gaatccctcc cgctccgcca gcccactttt gcccccctct cgtagtacgg
     2641 ttttgaacca agcaaaccgg cggttttccg ccagtcttag tgcgtctcct tgatgcgcca
     2701 gagaaccgcg ccgcgacctc aatgggcggc cccggcggcc atccaggcgg agagaatttt
     2761 ctcagttcag ttccgtggtc tgttctgccg cagcgtaggg cggatgggtc cgggtgagag
     2821 gtatggaaac cgcgtgacat tgccgggtga tcgagcaaac tgtacgcgaa aatggcgcga
     2881 aggtcagaaa gcggcagttt ccgggagttt ttcagggacg ggccgccggg ataacagggg
     2941 ccgagttgga gcataacagg ccccgagttc gctcataaca ggcactgagt tgagcaatag
     3001 caggcactga gttcgcggcc gggcaggctt cttccgcgga gctgcgatgt tgggctgatc
     3061 attccgcggg cccctccgtt ccgctttcgc cacagcggca aattttgcgt cggcaaggtc
     3121 gtgacga
//
LOCUS       DATNBV010000334         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3397793,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000334 DATNBV010000000
VERSION     DATNBV010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3397793"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VN627_08000"
     CDS             complement(<1..232)
                     /locus_tag="VN627_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03234.1"
                     /translation="MRLWTGDDQNSHFEDGILDLEPGPRGDLLSSKLAAAAVSFQETV
                     SGGAFAWHTAPARQLVITLAGTLDFQTREGEHF"
     gene            complement(450..1424)
                     /locus_tag="VN627_08005"
     CDS             complement(450..1424)
                     /locus_tag="VN627_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXD03235.1"
                     /translation="MSDLKPESKPDTPEADGGAFPAAARPAPQTARRRIRVIPFLITL
                     ATIAIAAPLCWATWKAYMGTPWTRDGTVRVYVVTMAAEVSGRIVELPVTDNQFVHKGD
                     LLMVIEPTNYKIAVSLAEAAVQQADANAQNAAREAKRRQRLSTLEVTVEEQQTFAASA
                     TAAAAQYQQSLANLDQARVNLERTQIRSPVNGWVTNLRAQIGDYVNIGQNEISLVDAD
                     SFWVDGYFEESNLASIQEGDPAKIKLMGYNQIVTGHVGSVARAINVPNAQPDQAGLAS
                     VNPIFTWVRLAQRVPVRIDIDRVPDGVRLVAGMTATVQIDPPPRPAPK"
     gene            complement(1432..1635)
                     /locus_tag="VN627_08010"
     CDS             complement(1432..1635)
                     /locus_tag="VN627_08010"
                     /inference="COORDINATES: protein motif:HMM:NF019484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HXD03236.1"
                     /translation="MNFSEVNLFGVYVAPISVMMVATWVVLIALRRVAAHFGLLRYVW
                     HPALFVFSVYLILLSFLVLFLAR"
     gene            complement(1632..>2215)
                     /locus_tag="VN627_08015"
     CDS             complement(1632..>2215)
                     /locus_tag="VN627_08015"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HXD03237.1"
                     /translation="VTLSFVPLLAPANQMTYDPQEFFNAALAIVAGVGAGALSFRLLP
                     PLSPALRARRLLALSLRDMRHLAAGAVRWTPDDWKSLIYSRLSALPAQAEPLQRAQLL
                     AALSLGSEIMRLRRIARRFDLEEELGPAFEAVARGDSKFAREHLAILDRRLAMPSEAG
                     SGASIRLRGRGRILAMSELLTQHAPYFDSGPVR"
BASE COUNT          409 a          723 c          709 g          374 t
ORIGIN      
        1 ggaaatgctc cccctcccga gtctgaaaat ccagcgttcc ggccagggtg atgaccagtt
       61 ggcgcgccgg cgcagtgtgc caggcaaagg cgccgcccga tacggtttcc tggaaggaga
      121 cggcagccgc ggccagcttg ctgctcagca gatcgccccg cggccccggc tccagatcga
      181 ggatgccatc ctcgaaatgc gagttctggt catcaccggt ccataggcgc acacatcgga
      241 tcatctgcct gctcccgccg tgcttgctgt ggacttcgca ccggcgccgc catcggcaag
      301 ggccgatcga catgcagcga cggtctctcg cccgccccac ccgatgatcc tgcggcgcat
      361 attcggcggg ctcgatgggg aatactacgc ggggaccgca gggccgatct acccggaatg
      421 cacgatcacc agcgaattcg cattttgttt cacttcggcg ccggcctggg tggagggtcg
      481 atctgcacgg tggccgtcat gccggccacc agccgcacgc catccggcac ccggtcgata
      541 tcgatacgca ccggcacgcg ttgggcgagg cgcacccagg tgaaaatcgg attcaccgag
      601 gcgagccccg cctgatccgg ctgagcgttc ggaacattga tggcgcgagc gacgctgccg
      661 acatggccgg taacgatctg gttgtagccc atcagcttga tcttcgcggg gtcgccctcc
      721 tgtatcgagg caagattgct ttcctcgaaa tatccgtcga cccaaaacga atcggcatcg
      781 acgagggaga tttcgttctg tccgatattc acatagtcgc cgatctgcgc ccgcagattg
      841 gtgacccatc cattgacggg ggagcggatc tgggtgcgtt ccaggttcac ccgcgcctgg
      901 tccagattgg ccaggctctg ttgatattgg gccgcggcgg ccgtggcgct ggccgcgaag
      961 gtctgctgtt cctccaccgt gacttcaagg gtgctcaatc gctggcggcg ctttgcctcc
     1021 ctggccgcgt tctgcgcatt cgcatccgcc tgctggaccg ccgcctcggc caggctgacg
     1081 gcgatcttgt agttggtggg ctctatcacc atcaggaggt cgcccttgtg cacgaactga
     1141 ttgtcggtga ccggcagctc gacgatccgc ccgctgacct ccgcggccat ggtgacgaca
     1201 tagacccgca ccgtgccatc gcgcgtccag ggcgttccca tataggcctt ccacgtcgcc
     1261 cagcagagcg gcgccgctat cgcgatcgtc gccagggtga tgagaaaggg aatgacccgt
     1321 atgcgccgcc tggccgtctg cggtgccggg cgcgcggcgg caggaaacgc accgccatcg
     1381 gcttccgggg tatcgggctt cgattcgggt ttgagatcgg acatggcgag ctcaccgcgc
     1441 aaggaaaagc acaaggaacg aaagcaggat cagatagacc gagaagacga acagggcggg
     1501 atgccataca tatcgcaaca gcccgaaatg agcggcgacc ctgcgcaacg cgatgagaac
     1561 gacccaggtc gccaccatca tcacggaaat gggcgccaca tagacgccaa acaggttgac
     1621 ctcgctgaag ttcatcgcac cggcccggag tcgaaataag gcgcatgctg ggtcagcagc
     1681 tccgacatcg cgaggatcct gcctcgcccc cggagcctga tcgatgctcc ggagccggct
     1741 tcggacggca tcgcgagccg acggtcgagt atcgcaagat gttccctcgc aaacttgctg
     1801 tccccgcggg ccacggcttc aaatgccggc ccgagttcct cctcgagatc gaaccggcgt
     1861 gcgatgcggc gaagacgcat gatctcgctg cccagcgaca gcgccgccag aagctgggcg
     1921 cgctgcagcg gctcggcctg tgccggcaat gccgatagac ggctgtagat cagggatttc
     1981 cagtcatccg gcgtccagcg aacagcaccc gccgcgaggt gccgcatatc gcgcaggctg
     2041 agcgccagga gccggcgggc tcgcagggcc ggcgacagcg gcggcagcag gcgaaacgag
     2101 agtgctccgg cccccacccc ggcgacgatc gccaaggccg cgttgaagaa ttcctggggg
     2161 tcgtaggtca tctggttggc gggtgcgagc aatggcacaa agctcagggt caccc
//
LOCUS       DATNBV010000335         3288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2403212,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000335 DATNBV010000000
VERSION     DATNBV010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2403212"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /gene="hutU"
                     /locus_tag="VN627_08020"
     CDS             <1..905
                     /gene="hutU"
                     /locus_tag="VN627_08020"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HXD03238.1"
                     /translation="PDAVTDQTSAHDPLNGYLPAGWSLDRAERLRVSDPERVIKAAKE
                     SMAVQVRAMLEFHRRGIPTLDYGNNIRQMALEMGVADAFDFPGFVPAYIRPLFCRGIG
                     PFRWAALSGDPEDIYRTDQRVKELMPDDKHLHNWLDMAAKRIQFQGLPARICWVGLGD
                     RARLGLAFNEMVAKGEVKAPIVIGRDHLDSGSVASPNRETEAMMDGSDAVSDWPLLNA
                     LLNTASGATWVSIHHGGGVGIGYSQHAGMVIVADGTKDAARRLERVLTNDPATGVMRH
                     ADAGYDIAKTCAREQGLNLPMLED"
     gene            complement(1068..1844)
                     /locus_tag="VN627_08025"
     CDS             complement(1068..1844)
                     /locus_tag="VN627_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03239.1"
                     /translation="MGAPPLREPLQWTVNKPGTNNKKIGLQVASMTGPRGEFSIPPGD
                     YVVTVKQREAIVSQPLTVGSGGVTKTMVINTSIVAVRMIPYTGGKIVTEPVHWEVFQT
                     ALGRPGPDSKVADAVAPEASFTLAAGYYVVRSHYHDASSDLAMLVEPAVSYHYTVDLY
                     AATVVGRAVAPSGSSANDIKWQVVRSTAEPDGTHHVVATDSGANPTFLVREGNYLVIA
                     TGADGSVGEAPLAVVAGRTHQVKVILKKGASATVKTSTAG"
     gene            1843..2217
                     /locus_tag="VN627_08030"
     CDS             1843..2217
                     /locus_tag="VN627_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03240.1"
                     /translation="MMMAACKVTLTGVKEAASVGWGGKAATWFWVAEVLLAVEAASAV
                     DAALPVVALAVVALLVVVVPATALVLWLALGAVATAGFALPGFRLGTFSGQGVATITG
                     SGAGAGAGAGWAATGSGAGSGV"
     gene            complement(2442..3155)
                     /locus_tag="VN627_08035"
     CDS             complement(2442..3155)
                     /locus_tag="VN627_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03241.1"
                     /translation="MHGDILLIAHSHWCAGRLKPLLAAKGYRITWCCPAREDCLPSAH
                     GDFAGTIVLGGPESANDVDSKPYLRRELEWIERHVADDHRFLGICLGGQLLARALGAR
                     VDRHPDGLSEIGYYPIHPTASGAPLIPAGLRVYHWHKEGFELPSGAELLACGEAFPHQ
                     AYRYGSKIYGLQFHPEVTPTVAATWMKSASDWASRPGAQSEAEQMAGSSMFDAPLHDW
                     FDGFLDHWLAPAEARAAAE"
BASE COUNT          545 a         1094 c         1112 g          537 t
ORIGIN      
        1 gtcccgatgc ggtgacggac cagacctccg cccatgatcc cctcaacggc tatctgccgg
       61 cgggctggag cctggaccgg gcggagcggc tgcgggtcag cgatcccgag cgggtgatca
      121 aggcggccaa ggaatccatg gcggtccagg tgcgggcgat gctggagttc caccgccgcg
      181 gcatcccgac tctcgactac ggcaacaaca tccgccagat ggcgctggag atgggcgtgg
      241 ccgatgcctt cgatttcccc ggcttcgttc ccgcctatat ccgcccgctg ttctgccgcg
      301 gcatcggccc cttccgctgg gccgccctgt cgggcgatcc cgaggatatc taccgcaccg
      361 atcagcgggt gaaggagctg atgcccgacg acaagcatct gcacaactgg ctcgacatgg
      421 ccgccaagcg catccagttc cagggcctgc cggcacgcat ctgctgggtc ggtctcggcg
      481 atcgggcccg cctcggcctc gccttcaacg agatggtggc caagggcgag gtcaaggcgc
      541 ccatcgtcat cggccgcgac cacctcgact caggctcggt cgccagcccc aatcgcgaga
      601 ccgaggccat gatggacggc tccgacgccg tctccgactg gccgctgctg aacgccttgc
      661 tcaacactgc ctccggcgcc acctgggttt cgatccatca cggcggcggc gtcggcatcg
      721 gctattcgca gcacgccggc atggtcatcg tcgccgacgg caccaaggat gccgcccgaa
      781 ggctcgagcg cgtgctcacc aacgatcccg ccaccggcgt gatgcgccat gccgatgccg
      841 gctacgacat cgccaagacc tgcgcccgcg aacagggtct gaacctgccg atgctggagg
      901 attgaacgca cgaagcatcc actctccccg tcatggccga gcgaagtccc ggccatgacg
      961 acttgggcag tgaggctggc ctcaaaccgc agagccgatg tcaaagccca aaagaaaaag
     1021 gccccaggca acgcccgggg cctttgatct tcggtcgctg aacggtctca gccggcggtg
     1081 ctggtcttga ccgtggcgct ggcgcccttc ttcaggatca ccttgacctg atgggtgcgg
     1141 ccggcgacga cggccagcgg cgcttcgccc accgacccgt ccgccccggt cgcgatcacg
     1201 agataattgc cctcgcggac gaggaaggtc gggttggcgc cggaatccgt cgccacgacg
     1261 tgatgggtgc cgtccggctc ggcggtgctg cgcaccacct gccatttgat gtcgttggcc
     1321 gaggacccgg agggtgccac ggcgcggccg acgacggtcg cggcgtagag gtcgacggta
     1381 tagtggtagc tcactgccgg ctcgacgagc atggcgagat cggagctggc gtcgtgataa
     1441 tgggaccgca cgacgtaata gcccgccgcc aaggtgaagc tcgcctccgg cgccacggca
     1501 tcggcgacct tcgaatcggg gcctggcctg ccgagggcgg tctgaaacac ctcccaatgc
     1561 accggctccg tcacgatctt gccgccggta tagggaatca ttcggacggc gacgatcgag
     1621 gtattgatca ccatcgtctt ggtcacgcca cccgagccaa cggtcaaggg ctggctgacg
     1681 atggcctcgc gctgcttgac cgtgacgaca tagtcgcccg gcgggatcga gaattcgccc
     1741 cgcggcccgg tcatggaggc gacctgcagg ccgatcttct tgttgttggt accaggcttg
     1801 ttgacggtcc actgaagggg ctcgcgcagc ggcggcgcgc ccatgatgat ggccgcctgc
     1861 aaggtcacct tgacgggggt caaggaagcc gcatcggtcg gctggggcgg caaggcggcg
     1921 acctggttct gggtggccga ggtcttgctg gccgtggagg ccgccagcgc ggtggatgcc
     1981 gcgctacccg tcgtcgcgct ggccgtcgtc gcgctcttgg tggtcgtggt gccggcgacc
     2041 gccttggtgc tctggctggc gctcggcgcc gtggccacgg ccggtttcgc gctgccgggc
     2101 ttcaggctcg gcacgttctc gggccagggc gtggcgacca tcacgggctc cggcgccggc
     2161 gcgggcgccg gggcgggttg ggcggccacc ggctccgggg ccggcagcgg cgtttgattc
     2221 ggcgaggtca ccaaggaggc gtcggggtga atcgggacac tcgacccgtc attcccggcg
     2281 ctggcaaccg ttgcaaaact ggtggctaac aatgcggcta ggagaagcgc tcgcaccctt
     2341 ccctccgtga cgacctgttg tactctatgg gccctacata gcgcattgca tgctttggca
     2401 acaagcgttt cggtgcaagc ctttgtagtt cccctccgcg ctcactcggc ggcggcgcgc
     2461 gcctcggctg gcgccagcca atgatcgagg aatccgtcga accaatcatg cagcggcgca
     2521 tcgaacatgc tgctgccggc catctgctcg gcctcgctct gagccccggg acggctcgcc
     2581 caatcggacg cggatttcat ccaggtggcg gccaccgtgg gggtcacttc ggggtgaaac
     2641 tgcaacccat agatctttga tccataacga taggcctgat ggggaaaggc ctcgccgcac
     2701 gccaacaact ccgccccgga gggaagctcg aacccctctt tatgccagtg atagacgcgc
     2761 aagcccgcgg gaatcagcgg agcgccggaa gccgtgggat gaatcgggta gtagccgatc
     2821 tcgctcaggc catcggggtg gcgatcgacg cgcgcgccca gggcccgggc caggagctgg
     2881 ccaccgaggc agatgccgag gaatcgatga tcgtcggcga catggcgctc gatccactcg
     2941 agttcccggc gcagataggg cttcgaatcg acatcattgg cgctctcggg gccgcccagc
     3001 acgatggttc cggcgaaatc gccatgggcg gaggggaggc aatcctcccg ggccgggcag
     3061 caccaggtga tgcgatagcc cttggcggcg agcagcggct tgaggcgccc cgcgcaccag
     3121 tgactatggg caatgagaag tatgtcccca tgcattccgg gcaaatctct tatcagatca
     3181 gcgccccgcg agaaaggccg ccatatgaaa agccatctgc gcgggcggat aggcatgctc
     3241 ccggccgatg gggcaggcca gccgggcgag gcagccctgc ccgcggca
//
LOCUS       DATNBV010000336         2603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3684448,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000336 DATNBV010000000
VERSION     DATNBV010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3684448"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VN627_08040"
     CDS             <1..484
                     /locus_tag="VN627_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03242.1"
                     /translation="LYPGRQLGRFAASSLGVDAVLPSIRLKAIRRGIEDAGLIALAAR
                     EQPDETARIVARAVPAALDEADRNRPASWESAPVSFAAARAALREIVKRDAPMGEGEI
                     RAAFADLAARRARDVPLVARHHARPQRKVRTAALVAAGAVLAFGIAARLRFNRRMRRA
                     "
     gene            484..1446
                     /locus_tag="VN627_08045"
     CDS             484..1446
                     /locus_tag="VN627_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXD03243.1"
                     /translation="MLRILLEAFHFLAGFLNVFHAVIGALLITWVVLRVRKPEAARRF
                     GRWTLIAVGALLVLAPFAWIIPAVFKTGDAFNDYIFFPPLSKWGETLSLTNFHELFKG
                     KPSQRGAVYFWEYFLNSTVIATVTTTMQIFLSSLAGYALAKFEFRGKAVLTLFMLGAM
                     MIPPVLLLAPLYKMVVDLGLVDSHAGVIIPLMASTYGTFLFRQACVTIPNEMIDAARL
                     DGCSELGIYFRVVMPLVRPMAAAFCLVAFLANWNAFFQPNIFLHSDEKLTVPVALNLL
                     LGVYRQKQGVYLAGTALAMIPPAVLFFFLQKEFISGLTSGAVKG"
     gene            1527..2243
                     /locus_tag="VN627_08050"
     CDS             1527..2243
                     /locus_tag="VN627_08050"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HXD03244.1"
                     /translation="MTKTQFAIATVLLAAATGTASAQAPGAKPMAAPAAAPAAAPAAK
                     PAAAPAAAPAAKPAAAAPAAAPAAAPAAPAAAAAPPKPTPSKELEAFMKPFVGSWKCE
                     TKFAAGAMGPGSPEANAKSSVKFSKDLNGFFYRGDYEIKKQKGFDMPMKGTFYIGWDP
                     GTQEIITTGVDTAGGLGTGAGKITGDTVTYTGESRMMGMKMKTRETMGMKPGGKEGFH
                     KIEIDMGKGFMPMGEDTCKK"
     gene            2354..>2603
                     /locus_tag="VN627_08055"
     CDS             2354..>2603
                     /locus_tag="VN627_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03245.1"
                     /translation="MGNISRAAFGVLALGAVASAGCFDSGMRVPPQPPEPAELDLSVT
                     YQRIDGFGASSAWTANNISDALADQFFSPDTGIGLSLLR"
BASE COUNT          394 a          868 c          881 g          460 t
ORIGIN      
        1 gctctatccg gggcggcagc tggggcgctt cgccgcctcg tcgctcggcg tcgacgccgt
       61 gcttccgtcg atccggctca aggcgatccg gcgcggtatc gaggacgccg gcctgatcgc
      121 gctggcggcg cgcgagcaac cggacgagac cgcgcgcatc gtcgcgcgcg ctgtgcccgc
      181 cgcgctcgac gaggcagacc ggaaccgacc cgcgtcctgg gaatcggcgc cggtctcttt
      241 cgccgcagcg cgcgccgctc tgcgggagat cgtcaagcgc gatgcgccga tgggggaggg
      301 ggagattcga gctgcgttcg cggacctcgc ggcgcggagg gcccgggacg tcccgctggt
      361 ggcgcgccac cacgcccgcc ctcagcggaa ggtgcggact gccgccctgg ttgcggcggg
      421 cgcggtgctg gctttcggga tcgcggcgcg cctccgcttc aaccgccgca tgaggagggc
      481 gtgatgcttc gcatcctgct ggaagccttc catttcctcg ccggctttct gaacgtcttc
      541 cacgcggtca tcggcgctct gctgatcaca tgggtcgtcc tgcgcgtgcg caagcccgaa
      601 gcggcgcgcc ggttcgggcg ctggacgctg atcgcggtgg gcgcgctgct cgtcctggcg
      661 cccttcgcct ggatcattcc ggcggtgttc aagaccggcg acgcgttcaa cgactacatc
      721 ttctttccgc cgctctcgaa gtggggcgag acgctgtcgc tcaccaactt ccacgagctg
      781 ttcaagggca aacccagcca gcgcggcgcg gtctacttct gggagtattt cctgaactcc
      841 accgtgatcg cgacggtgac gacgacgatg cagatcttcc tttcgtcgct ggccggctac
      901 gcgctcgcga agttcgagtt ccgcggcaag gccgtgctga cgctgttcat gctcggcgcg
      961 atgatgattc cgccggtcct gttgctcgcg ccgctgtaca agatggtggt cgatctgggc
     1021 ctggtcgatt cgcacgccgg cgtgatcatc ccgctgatgg ccagcaccta cggcaccttc
     1081 ctgttccggc aagcctgcgt gacgattccc aacgagatga tcgatgccgc tcggctggac
     1141 ggctgcagcg agctcgggat ctacttccgc gtggtgatgc cgctggtgcg gccgatggcg
     1201 gcggcgttct gcctggtggc gttcctggcc aactggaacg cgttcttcca gccgaacatc
     1261 ttcctgcaca gcgacgagaa gctgaccgtg ccggtggcgc tgaacctgct cctcggcgtc
     1321 taccgccaga agcagggcgt ttacctggcg gggacggcgc tcgcgatgat tcccccggcg
     1381 gtgctgttct tcttcctcca gaaggagttc atcagcggtt tgacctccgg cgcggtcaaa
     1441 ggctgattcg cttcgaaccc agcgaaacgc tttgactaaa gattggatgg gggtactttc
     1501 ccccttcaat ttcagggagg ctccccatga ccaagaccca gttcgcaatt gctaccgtcc
     1561 tgctggctgc ggccaccgga acggcgtcgg ctcaggcccc gggcgcgaag cccatggccg
     1621 cgcccgctgc ggccccggcc gctgctcccg ctgcgaagcc ggctgccgct ccggccgccg
     1681 cgcccgccgc caaaccggct gctgctgcgc ccgctgctgc tccggccgcc gcgcccgccg
     1741 ctccggccgc cgccgccgcg cccccgaagc cgacgccctc gaaggagttg gaggcgttca
     1801 tgaagccgtt cgtgggcagc tggaagtgcg agacgaagtt cgccgccggc gcgatgggtc
     1861 ccggttcgcc cgaggccaac gccaagtcct ccgtgaagtt cagcaaggac ctgaacgggt
     1921 tcttctaccg cggcgactac gagatcaaga agcagaaggg cttcgacatg ccgatgaagg
     1981 ggacgttcta tatcggctgg gatcccggca cccaggagat catcacgacc ggcgtcgaca
     2041 cggccggggg cctcggcacg ggcgcgggca agatcaccgg cgacaccgtg acctacacgg
     2101 gcgagagccg gatgatgggc atgaagatga agacccgcga gacgatggga atgaagccgg
     2161 gcggcaagga aggcttccac aagatcgaga tcgacatggg caagggcttc atgccgatgg
     2221 gcgaagacac ctgcaagaag tagccgtagt cctcggcgga agtttgctgg gctgaaagaa
     2281 agacccgggc ggcgcctcta atgtgatcag gccgccgccc ggctgctaag attccctcct
     2341 tcgtatgact agcttgggta acatttcgcg cgccgcgttt ggcgtgctgg cgctgggggc
     2401 ggtggcgagc gccgggtgtt tcgactcggg catgcgcgtg ccgccgcagc cgcccgagcc
     2461 ggcagagctg gatctctcgg tcacgtatca gcggatcgac ggcttcggcg cgtcgagcgc
     2521 gtggacggcc aacaacatca gcgacgcgct cgcggatcag ttcttctcgc ccgataccgg
     2581 gatcggtctt tcgttgctgc gca
//
LOCUS       DATNBV010000337         2068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4023932,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000337 DATNBV010000000
VERSION     DATNBV010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4023932"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VN627_08060"
     CDS             <1..322
                     /locus_tag="VN627_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03246.1"
                     /translation="DLALNQAIAGVRARSGIVIVVAHRPSALAEIDKLLWLDNGVVRA
                     FGPKAEILPRLTGAPPPEQGKPSTPQAAPAVPAGTINVRAPARPASPAGDPAAPSPAN
                     EATA"
     gene            379..1650
                     /locus_tag="VN627_08065"
     CDS             379..1650
                     /locus_tag="VN627_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HXD03247.1"
                     /translation="MLYKTMRRSRMILGALVLGLALPMAVIPISGAVIASGEVSVASK
                     VKKIAHPRGGVIAEIPVSNGTRVKAGQLLMRLDTNVSAASAEMTVESIDQLRAREARL
                     RAERDALPGIDFPADLLARQAEPLVAQAIGEERRIFALDRQTVAGQVAAIQAQIAQAG
                     KAAGNYRVQADVYRQQADLIAEERRANDELWEKRYTTLQRRNELARAAVGLGGSVASA
                     EAQASQLGSKVAELRQQMIVIAQDSRARAGAELAQVQSKLIELKQNNVVAQDANERNL
                     IRAPYAGVVDKLAFTTIGGVIPAGETIMEIVPDKDPYVVTARVSPADVDELTTGQKVL
                     IRFSAFNSRTTPELEGRLTRIGADRTVDQQRGMAFYTVEAEVLPGELAKLGELKLRPG
                     MPAEAFIQTGSRTMLSYLFKPLSDQFARAFR"
BASE COUNT          377 a          764 c          635 g          292 t
ORIGIN      
        1 cgatctcgcg ttaaaccagg ccatcgctgg tgtccgggcg cgcagcggca tcgtcatcgt
       61 cgtcgcccac cggcccagcg cccttgctga gatcgataag ctgctgtggc tggacaacgg
      121 cgtggtccgc gccttcggcc cgaaggccga gatcctgccg cggttgaccg gcgccccacc
      181 gccagagcag ggcaagccat ccaccccgca ggccgccccc gccgtccccg cggggacgat
      241 caacgtccgc gcacccgcaa ggcccgcctc gccagccggc gatcccgcgg ccccgtcccc
      301 tgccaacgaa gcgaccgcat gatgttcggc tccaagcccc ttgcccttcc tgactattcc
      361 cggccccgcc aggagatcgt gctgtacaag acgatgcgcc gctcgcggat gatcctcggc
      421 gcgctggtcc tcggcctggc cctgccgatg gcggtgatcc cgatctcggg ggccgtcatc
      481 gccagtggcg aggtgtccgt cgccagcaag gtcaagaaga tcgcccatcc gcgcggcgga
      541 gtcatcgccg aaatcccggt cagcaacggc acgcgggtca aggccggcca gctcctcatg
      601 cggctcgata ccaacgtctc cgccgccagc gccgagatga ccgtggagtc catcgaccag
      661 ctgcgcgccc gcgaagcgcg cctcagggct gagcgcgatg ccctgcccgg gatcgacttc
      721 ccggccgatc tcctcgcccg ccaggccgaa ccgctggtcg cccaggcgat cggcgaggaa
      781 cgccgcatct tcgcgctcga ccggcagacc gtcgccgggc aggtcgccgc gatccaggcc
      841 cagatcgccc aggccggcaa ggccgcgggc aattaccgcg tccaggccga cgtctatcgc
      901 caacaggccg atctcatcgc cgaggaacgc cgcgccaacg atgagctgtg ggagaagcgc
      961 tacaccacgc tccagcgccg caacgaactg gcccgcgccg cggtcggcct cggcggctcg
     1021 gtcgcctcgg ccgaggccca agcctcgcag ctcggctcca aggtcgccga actgcgccag
     1081 cagatgatcg tgatcgccca ggattcgcgc gcccgcgccg gggccgagct ggcccaggtc
     1141 cagtccaagc tgatcgaact caagcagaac aacgtcgtcg cccaggacgc caacgagcgc
     1201 aacctgatcc gcgcgcccta tgccggcgtg gtcgacaagc tggccttcac caccatcggc
     1261 ggagtcatcc cggccggcga aacgatcatg gagatcgtgc ccgataagga tccctacgtg
     1321 gtcaccgccc gcgtcagccc ggccgatgtg gatgagttga ccaccgggca gaaggtgttg
     1381 atccgcttct ccgcgttcaa ctcgcgcacc acgcccgaac tggaaggccg cctcaccagg
     1441 atcggggctg accgcacggt cgaccagcag cgcggcatgg ccttctacac cgtcgaggcc
     1501 gaggtcctgc ccggcgagct cgccaagctt ggcgaactca agctgcgccc cggcatgccc
     1561 gccgaagcct tcatccagac cggctcgcgg accatgctct cctacctgtt caagccactg
     1621 tcggaccagt tcgcgcgggc ctttcgctga acctcgagaa gtcgagcaat cacgtgctac
     1681 gtcccggatg catgctcaat cggcgggttt ccggaacgaa ccccggaaag gtgccattcg
     1741 cggccgagca aggcagtgtc ggcgtaaccg ggacggggta gcgcccgcga gccggcgtcg
     1801 gcgctcacac gcagccgctg gcgggcagat tacgaccgcg acatcagcca atctttggca
     1861 aaacttcatt tgctttcaat cgcaaaaagg caggaattct gaggtccagg cacaagacgt
     1921 gccgtgcccg gagtagcagc gggcgtgccc gcataagcac cgtcaacgag ccccaccagt
     1981 tcacgcgcgg ctgcttacat ctagtcacaa ggaagccgac acatgacaga aagcaacatg
     2041 aacaagcgcg gattgccaca agcgacct
//
LOCUS       DATNBV010000338         3933 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_205450,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000338 DATNBV010000000
VERSION     DATNBV010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205450"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VN627_08070"
     CDS             <1..546
                     /locus_tag="VN627_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03248.1"
                     /translation="PQPQPAVRPPPKAVPQPAPAPKPAPKAVPQPAPKPAPPPKPAAG
                     GGSRLRSDFLKGIGGGTTPGATAKAPPAQSFGPAVKAALSGAITRQLKPHWAAPQGAD
                     ADQLVTVLAWDLNADGTLAGSPRVVSQAGITDANRAQAQRHAEQAIRAVRLAAPFELP
                     ADLFPLWRHVASFRFDRKLSQ"
     gene            543..1925
                     /gene="tolB"
                     /locus_tag="VN627_08075"
     CDS             543..1925
                     /gene="tolB"
                     /locus_tag="VN627_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HXD03249.1"
                     /translation="MKFAPFALFALPLAAALAAQPAPAPAPAAGDGAAQPAEGLTGSV
                     SDETPWQDLGIAIPTFPTDRDVPTEASAGSTASLGRAMAGVITADLRNNGLFKPTGPD
                     SLPQPSFGQVVAPDFGVWSGRGAEMLVHGYVRGNADGTLTVGCYLYDVALKQQLVKGG
                     WQVGPGEWRRAAHKCADLVYSRLSGESPFFDSKIAYIAETGPKNHRMKRLAIMDSDGA
                     NHRYITTGQAIALTPRYSPDYRSILYLSYFNGFPRIYVYDLASGKQRLITQSKNPTFA
                     PRWSPDGRWILYSMAIGGNTDIYKVAADGSGTTLRLTNTPGINIGGSFSPDGSKIVFE
                     SDRSGAQQVYVMDADGSNQKRISFFGGRAATPEWSPRGDQIAFTHIAGNFRIAVMSPS
                     GGNMRDLTDSWQDEAPTWSPNGRIVQFFRTEKGSGRAGIWQVDLTGRNERKLPTPVDA
                     SDPAWGPVQP"
     gene            2117..2647
                     /gene="pal"
                     /locus_tag="VN627_08080"
     CDS             2117..2647
                     /gene="pal"
                     /locus_tag="VN627_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXD03250.1"
                     /translation="MPHSTEFKLGLKAGTLVLAASLALAGCAKKAPPALPPAPQPTTA
                     TEAPPPVSGPVAGSQADFAAQMMGQDTIYFDTDKYDVDQADAAALKSQAAWLARYPGK
                     RATIEGHCDERGTREYNLALGERRANAAKNYLASLGVDASRLTTISYGKERPVALGSD
                     EASWAKNRRAVTVTID"
     gene            2821..>3933
                     /locus_tag="VN627_08085"
     CDS             2821..>3933
                     /locus_tag="VN627_08085"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HXD03251.1"
                     /translation="MKPALPAILPAILPALLAAILLGSPAAAAPSSESVAAAALKAAP
                     VWDGHNDVPEQLRDRRKDLLTGFDFRDTTGTADAAAGRVAMHTDLTRLRRGHVGAQFW
                     SVFVPASLTDQQAVQAVMEQIDVTRRLIAAYPADLQYAGDAAGVAAAWKAGRIASLLG
                     MEGGYAIGNSLGVLRQFRELGVRYMTLTHYKTTSWADSATDAPQHGGLTDFGKQVVRE
                     MQRIGMLVDLSHVSAQSMNAALDVARAPVIFSHSGARAIGDHPRNVPDDVLVRLKANG
                     GIVMVVTLPAYVSNAVRLWELDRTAERARLGALHVDAPDLAKAALADWEQAHPRPRAR
                     LTDLADHIDHIVKLAGIDHVGLGGDFDGMDTTTEGMD"
BASE COUNT          624 a         1393 c         1338 g          578 t
ORIGIN      
        1 ccccagccgc agccggcagt gcggccgccg cccaaggcgg tgccgcaacc tgcccctgca
       61 ccgaaacctg ctcccaaggc cgtgcctcaa cccgcgccga agcccgcgcc gccgcccaaa
      121 ccggcagccg gcggtggcag ccggctgcgc agcgacttcc tcaagggcat cggcggcggc
      181 accacgccgg gggccacggc caaggctccg ccggcacagt cgttcggccc cgcggtgaaa
      241 gcggcgctgt caggtgcaat cacccggcaa ctgaagcctc actgggcggc gccgcagggg
      301 gccgatgccg accagctggt caccgtgctg gcctgggacc tgaacgccga cggcacgctg
      361 gccggatcgc cgcgcgtggt cagccaggcc ggcataaccg atgccaaccg ggcccaggcg
      421 cagcgccacg ccgagcaggc gatccgcgcg gtgcgcctgg cagcgccgtt cgaactgccc
      481 gccgatcttt tccccctgtg gaggcatgtt gcctccttcc gcttcgacag gaagttgtcg
      541 caatgaaatt cgctcccttc gccctgttcg ccctgccgct ggctgccgcc ctggcggcgc
      601 agcccgcacc tgctcccgcc cccgccgcgg gtgatggcgc ggcgcagcct gccgaagggc
      661 tgaccggctc ggtctccgac gaaaccccat ggcaggacct cggcatcgcc atcccgacct
      721 tcccgaccga ccgcgacgtg ccgaccgagg ccagcgcggg atcgaccgcg tcgctcggcc
      781 gggccatggc gggcgtgatc acggcggacc tcaggaacaa cggcctgttc aagccgaccg
      841 ggcccgattc gctgccccag cccagcttcg ggcaggtggt ggcgcccgat ttcggggttt
      901 ggtctggccg gggcgcggaa atgctggtcc atggctatgt gcgcggcaat gccgacggga
      961 cgctgaccgt ggggtgctat ctctatgacg tggcgctcaa gcagcagctg gtgaagggcg
     1021 gctggcaggt cggcccgggc gagtggcggc gggcggcgca caagtgcgcg gacctcgtct
     1081 attcgcggct ctccggcgag agcccgttct tcgattccaa gatcgcctat atcgccgaga
     1141 ccggccccaa gaatcaccgc atgaagcggc tggcgatcat ggattcggat ggcgccaacc
     1201 accgctacat caccacgggc caggcgatcg cgctgacccc gcgctattcg cccgactaca
     1261 ggtcgatcct ttaccttagc tatttcaacg ggttcccccg gatctacgtt tatgacctgg
     1321 cctcgggcaa gcagcggctg atcacccaga gcaagaaccc caccttcgcg ccgcgctggt
     1381 cgcccgatgg ccggtggatc ctctattcga tggcgatcgg cggcaatacc gatatctaca
     1441 aggtggccgc cgacggcagc gggacgaccc tgcggctgac caatacgccg ggcatcaaca
     1501 tcggcggatc gttctcgccc gacggcagca agatcgtgtt cgagagcgac cgttcgggcg
     1561 cgcagcaggt ctatgtgatg gacgccgacg gttcgaacca gaagcggatc agcttcttcg
     1621 gcgggcgcgc ggcaacgccg gaatggtccc cccgcggcga ccaaatcgcc ttcacccaca
     1681 ttgccgggaa ctttcgtatt gccgtcatgt ccccttctgg tggtaacatg cgcgatctga
     1741 ccgacagctg gcaggacgag gcgccgacct ggtcgcccaa tggccggatc gtccagttct
     1801 tccgcaccga gaagggcagc gggcgggccg gcatctggca ggtggacctg accgggcgca
     1861 acgagcgcaa gctgccgacg ccggtcgatg cttcggaccc cgcctggggg ccggtgcaac
     1921 cttaggttgc accgccattc cgcccagggc ggcctgcgaa tggcggttcg gcgcgcttcc
     1981 ggcgctcacg accagcaggt cgctgcgcgc cgggtcgcgc cgaaccacca ttttcgactc
     2041 gccctgaacg aaatggcaat gcaacctaag ccagtgatga cactggagcg cgaacgagat
     2101 ttgaaggaga cgacagatgc cgcattccac tgagttcaag ttgggcctga aggccggcac
     2161 gctggtcctc gccgccagcc tcgcgctggc cggctgcgcc aagaaggcgc ctcccgccct
     2221 gccgcccgct cctcagccga ccacggcgac cgaggcgccg ccgccggtgt caggtccggt
     2281 tgccggcagc caggcggact tcgccgccca gatgatgggg caggacacga tttacttcga
     2341 caccgacaag tatgacgtcg accaggccga tgccgccgcg ctcaagtcgc aggcggcctg
     2401 gctggcccgc tatccgggca agcgcgcgac gatcgagggc cactgcgacg agcgcggcac
     2461 ccgcgaatat aacctcgcgc tgggcgagcg ccgggccaat gccgccaaga attacctcgc
     2521 cagcctcggc gtcgacgcca gccgcctgac cacgatctcc tacggcaagg agcgcccggt
     2581 ggcgctcggt tcggacgagg cgagctgggc caagaaccgc cgcgcggtga ctgtcaccat
     2641 cgactgaagc tcctgcggct gcgaataacc ctccgtcccc atggggcggg gggttattcg
     2701 tgcggctgac gtttcgccgc ccgcccaggc gtggcgccgc agatgcgtgt tggcggcggg
     2761 cggggatcct gcaacattgt cctgcaagac tggacccagc cgcctggacg aggacgcccg
     2821 atgaagcctg ccctgcccgc gatcctgccc gcgatcctgc ccgccttgct ggccgccatc
     2881 ctgctcggct cgccggcggc cgcggcgccc tcgtcagaga gcgtggccgc cgcggcgctc
     2941 aaggccgcgc cggtgtggga cggccacaat gacgtgcccg aacagctgcg ggaccgccgc
     3001 aaggacctgc tcaccgggtt cgacttccgc gataccaccg gcaccgccga tgccgcggcc
     3061 gggcgcgtgg cgatgcacac cgacctgacg cgcctgcgcc ggggccatgt cggggcgcag
     3121 ttctggtcgg tcttcgtgcc ggcctcgctg accgaccagc aggccgtgca ggccgtgatg
     3181 gagcagatcg acgtgacccg gcggctgatt gccgcctatc cggccgacct ccagtatgct
     3241 ggcgacgcgg ccggcgtggc ggcggcgtgg aaggcggggc ggatcgcttc gctgctcggc
     3301 atggagggcg gctatgccat cggcaactcg ctcggcgtgc tgcgccagtt ccgcgagctg
     3361 ggcgtgcgct acatgacgct gacccactac aagaccacca gctgggccga cagcgcgacc
     3421 gatgccccgc agcatggcgg gctgaccgat ttcggcaagc aggtcgtgcg cgagatgcag
     3481 cggatcggca tgctggtcga cctcagccac gtcagcgcgc aatcgatgaa cgcggcgctc
     3541 gacgttgcgc gcgcgccggt gatcttcagc cattccgggg cgcgggcgat cggcgatcat
     3601 ccgcgcaacg tgccggacga cgtgctggta cggctgaagg ccaatggcgg gatcgtcatg
     3661 gtggtgaccc tgccggccta tgtcagcaat gccgtgcgcc tctgggagct ggaccgcacg
     3721 gcggagcggg cacggctggg ggcgctccat gtcgatgcgc cggacctggc caaggcggcg
     3781 ctcgccgact gggagcaggc ccatccgcgg ccgcgcgcca gactgaccga tctggccgac
     3841 catatcgacc atatcgtcaa gctggccggg atcgaccatg tgggcctcgg cggtgacttc
     3901 gacgggatgg atacgacgac cgaggggatg gac
//
LOCUS       DATNBV010000339         6917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2749469,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000339 DATNBV010000000
VERSION     DATNBV010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2749469"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..928)
                     /gene="sufC"
                     /locus_tag="VN627_08090"
     CDS             complement(188..928)
                     /gene="sufC"
                     /locus_tag="VN627_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HXD03252.1"
                     /translation="MLQIDNLKAEVVGKPILKGLSLTVNAGEIHAIMGPNGAGKSTTG
                     YVLGGRPGYAVTGGSVTFKGEDLLALEPHERAAAGLFLGFQYPVEIPGVSFVQFLREA
                     ANAQRRVRGQEPLSGGEFLKLAKEKAGLLRMDMDMLKRPVNVGFSGGEKKRAEMVQMG
                     ILDPKLAILDETDSGLDIDALRICGEGINAIMRRPDKAVILITHYQRLLDYVKPDKVH
                     VLAGGRIVRSGGPELAHELEREGYEAVA"
     gene            complement(931..1416)
                     /locus_tag="VN627_08095"
     CDS             complement(931..1416)
                     /locus_tag="VN627_08095"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HXD03253.1"
                     /translation="MSERKTLSVRETDETSPTVKKKGRGWEISEKRLDALHERAREMR
                     RNPTPAQEALAEALGGVENGGFAFKRQTVIGSAIVDFACKDLMLVIELAGDEDAAFAA
                     TRDRSLTEVGYRVERIGAEAVLADPAGAAAHVSAIMRERWHERRSRHRAQQPGRGRQQ
                     G"
     gene            complement(1416..2897)
                     /gene="sufB"
                     /locus_tag="VN627_08100"
     CDS             complement(1416..2897)
                     /gene="sufB"
                     /locus_tag="VN627_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HXD03254.1"
                     /translation="MTEQTDLKDQAARDAAARVAEYEHGWVSDIEQEYGPKGLSEDTV
                     RFISEKKGEPEWMLEWRLKAYRHWLTLPMPDWAKLKVPPIDYQEAYYWAAPKAKPKLG
                     SLDEVDPEILKVYEKLGIPLEEQKVLAGVEGARRVAVDAVFDSVSVATTFRKELEQAG
                     VIFRSISEAIREYPELVRKWLGKVVPMHDNFFAALNQAVFSDGTFVYIPEGVRCPMEL
                     STYFRINAENTGQFERTLIVADRRSYVSYLEGCTAPQRDENQLHAAVVELVALDDAEI
                     KYSTVQNWYPGDAQGKGGIYNFVTKRALCQGARSKVSWTQVETGSAITWKYPSCVLNG
                     VDSVGEFYSVAVTNNYQQADTGTKMIHNGKGSRSTIVSKGISAGKSNNTYRGLVRVAA
                     GADDVRNFTQCDSLLLGKDCGAHTVPYIEVRNPSAQIEHEATTSKISDDQLFYAMQRG
                     LGQEEAVALIVNGFAKEVLQQLPMEFAVEAQKLLGISLEGSVG"
     gene            complement(2894..3313)
                     /locus_tag="VN627_08105"
     CDS             complement(2894..3313)
                     /locus_tag="VN627_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HXD03255.1"
                     /translation="MRLSSMADYAVLTMSAAARHCGGARLNAAQLAEETGLPTPTVAK
                     LVSRLSAAGLLKSVRGAHGGFKLARPAAAITLADIVEAVEGPIALTACVETGKHDCTL
                     EAACAMRPHWPQVNEALRGALAAIALAELSAPAGAHA"
     gene            3665..4696
                     /locus_tag="VN627_08110"
     CDS             3665..4696
                     /locus_tag="VN627_08110"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HXD03256.1"
                     /translation="MLYSLLRPALFRLDAERAHGLALRALALRGGKARPPKTPALATE
                     VAGLSFPNPLGMAAGFDKDGEVPDALLGLGFGFAEVGSITPLPQAGNAKPRLFRLAED
                     RAVINRMGFNNGGAEAAARRLAARAHRPGIVGINIGANKDSADRVADYATMARLMAPL
                     AAYLAVNVSSPNTPGLRALQDESALSDLLDAVLAARGAGGPPVFLKVAPDLEPADIDA
                     IARIALDKKLGALIVSNTTISRPPLASRHAHETGGLSGAPLRDLAQQRLRDFRKATGG
                     ALPLVGVGGIATAEDAFARIRAGASLVQLYSAMVYAGPGIARTIVRGLDALVRQYGFS
                     TIAEAVGSE"
     gene            4708..6495
                     /gene="ggt"
                     /locus_tag="VN627_08115"
     CDS             4708..6495
                     /gene="ggt"
                     /locus_tag="VN627_08115"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXD03257.1"
                     /translation="MPELTRTRPALAAIALVLLAGAGPAAARPARAHGAHPARVQPLG
                     VVSAADPRAAAAGAEMLRRGGSATDAAIATMLALNVVEPQNSGLGGGLFYLRHDARTG
                     GLASIDGRETAPMAADPRWFYGADGQVLPQRELNIGGRSAGVPGALAAMALAHKRYGR
                     LPWAVLFQPAIRLAERGFTVSARMHNGLNLYGRHVEGWARKVYFTPGDQPVAKGTLLR
                     LPAMAATFRTIARKGPAPFYTGTMAARMVTALGTAARNPSPITPADFAAYRPRDRAPI
                     CGTYRQWKICGMGPPSAGAITVLMILGQLERFDLAAMGRVSPVAWHLFAESSRLAYAD
                     RAQYIADPDFVSVPAKGLLDPAYLAARSALISPERTLAMVEPGRPAGAPPRLKVAFRD
                     DPGTSDLAVADGRGEVVQVTTTINGYFGSGVAAEGLMLNNELPDFDPVPDKDGYLVAN
                     RVEGGKRPRSSMAPTIVFDPSGKQRLAIGAAGGATIIAQVAKALIGVIDWKLSAQDAI
                     AMGLVYAPTTGGTIEQGTELESLAPGLAPLGETLKVAPLGLKANAVELVGGQWRGAAD
                     PRSEGAAVDALGRVTRIERRENELNGAHE"
     gene            6609..>6917
                     /locus_tag="VN627_08120"
     CDS             6609..>6917
                     /locus_tag="VN627_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXD03258.1"
                     /translation="MQLTDFDAAPNLVALFLQRADELADKPFLHAKREGRWQAITWRE
                     AADRVCLIAEALRGMGLGAGDRIMLVSENRPEWCLADLAIMAAGLVTVPAYTTNTERD
                     H"
BASE COUNT         1024 a         2459 c         2301 g         1133 t
ORIGIN      
        1 ggagcgaggg tgatggcgag gtgtcgggcg accgcatcgc cttccggtac aagcaccagg
       61 gcgcggcttt cgcccgggcc gagggcgatt tcctccaccg gcacgggcca gacccgcgcc
      121 agggcgccga gatcggcgta gcggaagtcc tcgtccttac gcgacggaag caaggcgccg
      181 gcaaggctca tgcaacggcc tcgtaaccct cgcgctccag ttcgtgcgcg agttcggggc
      241 cgccggagcg gacgatgcgg ccgccggcga ggacgtggac cttgtcgggc ttcacataat
      301 cgagcaggcg ctggtagtgg gtgatcagga tcacggcctt gtcaggccgc ctcatgatcg
      361 cattgatccc ttcgccgcag atgcgcaggg catcgatgtc gaggcccgaa tcggtttcgt
      421 cgagaatcgc cagcttcgga tcgaggatgc ccatctggac catctcggcg cgcttcttct
      481 cgccgccgga aaagcccacg ttcaccgggc gcttgagcat gtccatgtcc atgcgcagca
      541 ggcccgcctt ctccttggcc agcttgagga actcgccgcc ggaaagcggc tcttgcccgc
      601 ggactctgcg ctgggcattg gccgcctcgc gcaggaactg gacgaaggag acgcccggga
      661 tctcgaccgg gtactggaaa ccgaggaaca agccggcggc cgcgcgctcg tgcggttcga
      721 gcgcgagcag gtcctcgccc ttgaacgtga ccgagccgcc cgtcaccgca tagcccggac
      781 gcccgcccag cacgtagccg gtggtcgact tgcctgcgcc gttcggcccc atgatcgcat
      841 ggatctcgcc cgcgttcacg gtgaggctga gccccttgag gatgggcttg ccgacaactt
      901 cggccttgag gttatcgatc tggagcatgg tcatccctgt tggcgcccgc ggcctggctg
      961 ctgcgcgcgg tggcggctgc ggcgttcgtg ccagcgttcg cgcatgatgg cggaaacgtg
     1021 ggcggcggcg cccgccggat cagccagcac ggcttcggcg ccgatccgct ccacccggta
     1081 gcccacttcg gtcaggctgc ggtcgcgcgt ggcggcgaag gcggcatcct cgtcgcccgc
     1141 aagctcgatc accagcatca ggtccttgca ggcaaagtcg acgatcgccg agccgatcac
     1201 ggtctgccgc ttgaacgcga acccaccgtt ctccaccccg cccagcgcct cggccagcgc
     1261 ttcctgcgcg ggtgtgggat tgcggcgcat ctcgcgcgcg cgctcgtgca gcgcatcgag
     1321 gcgcttctcg gaaatctccc aaccgcggcc ctttttcttg accgtggggg aggtttcatc
     1381 ggtctcgcga accgacaggg tcttgcgctc actcatcatc ccaccgatcc ctcaaggctg
     1441 atcccaagca gcttctgcgc ttccaccgcg aactccatcg gcagctgctg cagcacctct
     1501 ttcgcgaagc cgttgacgat cagcgccacg gcttcttcct gaccgagccc gcgctgcatc
     1561 gcgtagaaca gctggtcgtc gctgatcttg ctggtcgtgg cctcatgctc gatctgggcg
     1621 gaggggttgc gcacttcgat atagggcact gtgtgggccc cgcagtcctt gccgagcagg
     1681 agcgaatcgc actgggtgaa attgcgcacg tcgtccgccc cggcggcaac gcggaccagg
     1741 ccgcgatagg tgttgttcga ctttcccgcg ctgatgccct tggacacgat ggtcgaccgg
     1801 ctgcccttgc cgttgtggat catcttggtc ccggtatcgg cctgctggta attgttggtg
     1861 acggcgaccg aatagaactc gcccaccgaa tccacgccgt tgagcacgca gctcggatac
     1921 ttccaggtga tcgccgagcc ggtctcgacc tgggtccagc tcaccttcga acgcgcgccc
     1981 tggcacagcg cccgcttggt cacgaagttg tagatcccgc ccttcccctg cgcgtcgccg
     2041 gggtaccagt tctgcacggt cgaatacttg atctcggcat cgtcgagcgc gaccagttcg
     2101 acgacagcgg cgtgcagctg gttctcgtcg cgctgggggg cggtgcagcc ctccaggtag
     2161 gagacatagc tgcgccggtc ggcgacgatc agggtacgtt cgaactggcc ggtgttttcc
     2221 gcattgatgc ggaaataggt ggaaagctcc atcgggcagc gcacgccctc gggaatgtag
     2281 acgaaggtcc cgtcggaaaa gaccgcctgg ttgagcgcgg cgaagaagtt gtcgtgcatc
     2341 ggcacgacct tgccgagcca cttcctgacc agctcgggat attcgcggat tgcctcggaa
     2401 atcgagcgga agatgacccc ggcctgttcg agctccttgc ggaaggtcgt agcaaccgag
     2461 accgagtcga acaccgcgtc cacggccacc ctgcgcgcgc cttccacccc ggcgagcacc
     2521 ttctgctcct ccagcgggat gccgagcttc tcgtagacct tgaggatttc cggatcgact
     2581 tcgtcaaggc ttcccagctt cggcttggcc ttgggtgcgg cccagtaata ggcctcctgg
     2641 taatcgatcg ggggcacctt gagcttggcc cagtcgggca tcggcagggt cagccagtgg
     2701 cgataggcct tgagccgcca ctccagcatc cactccggct cgcccttctt ctccgagatg
     2761 aagcgaaccg tgtcttccga caggcccttg gggccgtatt cctgctcgat atcggagacc
     2821 cagccgtgct cgtattcggc gacgcgcgcc gcggcgtcgc gggcggcctg atccttgaga
     2881 tcggtctgct cggtcatgcg tgggctccgg ccggggccga cagttcggcc agggcgatag
     2941 ccgccagggc gccgcgcagc gcctcgttca cctggggcca gtgcgggcgc atcgcgcagg
     3001 ccgcctcgag cgtgcaatcg tgcttgccgg tctccacgca ggcggtgagc gcgatcggcc
     3061 cctccaccgc ctcgacgata tcggccagcg tgatcgccgc cgccggacgg gccagcttga
     3121 acccgccatg ggcgccgcgc accgacttga gcaggcccgc ggcggaaagc cggctgacca
     3181 gcttggcgac cgtgggcgtg ggcaggccgg tttcctcggc cagctgggca gcgttgagcc
     3241 gcgcaccgcc gcagtgccgc gcggcggcgg acatcgtcag gacggcatag tcggccatgc
     3301 tggagaggcg catggggatg aactccgggg gcgggagcaa atcggactga ttcgctccgg
     3361 ttattccgtc gggctactta ggcgcggcaa cggccgaata caactgctgc gaagaccaca
     3421 gaaaaaaggg cggcgcccgc gatccgcgaa ggaccgtacc ggcaccgccc atgaagttga
     3481 gagctcttca gcaggcaagc ctgcgcgtgg agcccttcgg gtcgcaactc cgccctaggc
     3541 cggacctgac cgtcatgaat tgtatgaatt cgacatgtta gccgtcgatc ggtcgatttt
     3601 tccgcgcaaa cccgcgcttt gcacccgtgt cgacagcact gccccgccct gccataggcc
     3661 gcccatgctc tattccctgc tgcgccccgc cctgttccga ctcgatgccg agcgcgccca
     3721 tggcctggcc ctgcgcgccc ttgcgctgcg cggcggcaag gcccggccgc caaagacgcc
     3781 ggccctcgcc accgaagtgg ccggcctctc ctttcccaat ccgctgggca tggccgccgg
     3841 gttcgacaag gacggcgagg ttcccgatgc cctgctcggc ctcggcttcg gcttcgccga
     3901 ggtcggctcg atcactccct tgccgcaggc cggcaacgcc aagccgcgcc tgttccggct
     3961 ggccgaggac cgcgcagtga tcaatcgcat gggcttcaac aacggcgggg ccgaggctgc
     4021 cgcccggcgc ctcgccgcgc gcgcccatcg ccccggcatt gtcggcatca atatcggcgc
     4081 caacaaggat tcggccgacc gggtggccga ctatgcgacc atggcgcggc tgatggcgcc
     4141 gctggccgcc tacctcgcgg tcaacgtctc cagccccaac acgccgggcc tgcgcgccct
     4201 gcaggacgaa agcgccctgt cggacctgct cgatgccgta ctcgccgcgc gcggagccgg
     4261 cggcccgccg gtgttcctca aggtcgctcc cgatctcgag ccggccgata tcgacgccat
     4321 tgcccggatc gccctcgaca agaagctggg cgcgctgatc gtctccaaca ccaccatctc
     4381 gcgcccgccg ctggcctcgc gccatgccca tgagaccggc ggcctgtccg gcgcgccctt
     4441 gcgcgatctg gcccagcagc gcctgcgcga tttccgcaag gcgaccggcg gcgccctgcc
     4501 gctggtgggc gtgggcggca ttgcgaccgc cgaagacgcc ttcgcccgca tccgcgccgg
     4561 ggccagcctg gtccagcttt acagcgccat ggtctatgcc gggcccggga tcgcccggac
     4621 gatcgtcagg ggcctcgacg cactggtccg gcaatatggc ttttcgacaa ttgccgaggc
     4681 cgtgggcagc gaatagcctg gcccgtcatg cctgagctca cccgcacacg cccggccctg
     4741 gcggccattg ccctggtcct gctggccggg gccggtccgg cggcggcgcg gccggcacgc
     4801 gcccatggcg cgcaccccgc ccgggtccag ccgctcggcg tggtctccgc cgccgatccc
     4861 cgcgccgcgg cggccggggc cgagatgttg cgcaggggcg gcagcgccac cgatgcagcg
     4921 attgccacca tgctcgccct caacgtggtc gaaccacaga actccggcct cggcggcggg
     4981 ctgttctacc tgcgccacga tgcgcgcacc ggcgggctcg cctcgatcga cggtcgcgag
     5041 accgctccca tggcggcaga tcccagatgg ttctacggcg ccgacggcca ggtgctgccg
     5101 caacgggaac tcaatattgg cgggcgcagc gccggcgtgc cgggggcgct cgcggccatg
     5161 gcgctcgcgc acaagcgcta tggcaggctg ccctgggccg tgctgttcca gcccgcgatc
     5221 cggctggccg aacggggctt caccgtttcg gcccggatgc acaacgggct caacctctat
     5281 ggccggcatg tcgaaggctg ggcccgcaag gtctatttca cgcccggcga ccagcccgtg
     5341 gccaagggca ccctgctcag gctgcccgcg atggccgcga ccttccggac catcgcccgc
     5401 aagggcccgg cgccattcta caccgggacg atggccgcgc ggatggtgac ggcgctaggc
     5461 accgccgcgc gcaatccctc gccgatcact cccgccgatt tcgccgccta ccggcccagg
     5521 gaccgcgcgc cgatctgcgg cacctaccgc cagtggaaga tctgcggcat gggcccgcct
     5581 tcggccgggg cgatcaccgt gctgatgatc ctgggccagc tcgaacggtt tgaccttgcc
     5641 gccatgggcc gcgtctcgcc ggtggcgtgg cacctgttcg ccgagagctc gcgcctcgcc
     5701 tatgcggacc gcgcgcaata tattgccgat ccggatttcg tcagcgtgcc ggccaagggc
     5761 ctgctcgatc ccgcctacct cgccgcgcga tcggcgctga tctcgcccga gcgcacgctg
     5821 gccatggtcg agccgggccg gccggcgggt gccccgccgc gcctgaaggt cgccttccgc
     5881 gacgatccgg gcaccagcga cctggccgtt gccgacggcc gcggcgaggt ggtccaggtc
     5941 accaccacga tcaacggcta cttcggctcg ggcgtcgcgg cggaggggct gatgctcaac
     6001 aacgagctgc ccgatttcga cccggtgccg gataaggacg gctatcttgt cgccaaccgc
     6061 gtggagggcg gcaagcgccc ccgcagctcg atggcgccga ccatcgtgtt cgatccttcc
     6121 ggcaagcagc gccttgccat cggcgcggcc ggcggggcga cgatcatcgc ccaggtggcc
     6181 aaggcgctga tcggggtgat cgactggaag ctctcggcgc aggacgccat cgccatgggg
     6241 ctggtctacg cgcccacgac cggcggcacg atcgagcagg gcaccgagct cgagtccctg
     6301 gcgccgggcc tcgccccgct gggcgagacc ctcaaggtcg ccccgctcgg tctcaaggcc
     6361 aatgccgtgg aactggtggg cggccagtgg cgcggggcgg ccgatccgcg cagcgaaggg
     6421 gcggcggtcg atgcccttgg ccgggtaacc cggatcgagc ggcgggaaaa cgaactcaac
     6481 ggcgcgcacg aatagcgcct ccctgccgcc ggaccgattg cgctagggcg ttgcccttgg
     6541 ccctgccggt gctagtctta gtcttgacgg gaacggcgcc gatctgggcc ggggggacga
     6601 ggaccgctgt gcagcttacc gatttcgatg ccgcgcccaa tcttgttgcc ctgttcctgc
     6661 aacgcgccga cgagctggcc gacaagcctt tcctccatgc caagcgcgag gggcgctggc
     6721 aggcgatcac ctggcgcgag gcggcggacc gggtctgcct catcgccgaa gcgctgcgcg
     6781 gcatgggact tggcgcgggc gaccgcatca tgctggtctc cgaaaaccgg cccgagtggt
     6841 gcctggccga tctcgccatc atggccgcgg gcctcgtcac ggtcccggcc tacaccacca
     6901 acaccgagcg cgatcac
//
LOCUS       DATNBV010000340         4098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3685165,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000340 DATNBV010000000
VERSION     DATNBV010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3685165"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..815)
                     /gene="lptB"
                     /locus_tag="VN627_08125"
     CDS             complement(27..815)
                     /gene="lptB"
                     /locus_tag="VN627_08125"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HXD03259.1"
                     /translation="MSDIADIPQADAANAGAMQPIPQGGLEVISIAKSYDKRAVLTDI
                     SLSVGKGEVLGLLGPNGAGKTTCFYSIMGLVRPDSGRILMDGIDVTNLPMYRRAILGL
                     GYLPQETSIFRGMTVEQNIASVLELVEPDRDVRAEELERLLTEFGLTRLRSSAAMALS
                     GGERRRCEIARALAAKPSIMLLDEPFAGIDPLSISDIRHLVIDLKQRGIGVLITDHNV
                     RETLDIVDRACIIYGGQVLFAGSPEALVADENVRRLYLGEGFTL"
     gene            1065..1376
                     /locus_tag="VN627_08130"
     CDS             1065..1376
                     /locus_tag="VN627_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03260.1"
                     /translation="MSNQVAYALLAYTALQIFLTVGALQGDRGSLLPYLALVVLVGAI
                     IPACRGCERRWQELEEGPAHDPALARCYRRDCKVLWALALGVPLAMTAVLKGLALVFA
                     G"
     gene            1450..2793
                     /locus_tag="VN627_08135"
     CDS             1450..2793
                     /locus_tag="VN627_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HXD03261.1"
                     /translation="MLDVSAAQERAAALIDRARRAGADAADAVYIADASESVAVRLGK
                     LEDLERSESEHVSLRVFAGKGSASIGSSDLGDAALDELAARAVAMARAAPEDSYAALA
                     PEDMLARGPLPELDLSDGGAVEPAQLRRTAEEVEAAALAVPGVSNSQGGSASAGRTVV
                     ALATSHRFAGGYETTSHGVSASVVAGEGAAMQRDYAWRSARHRTDLPGAAEIGRLAGE
                     RAVARLAPGRLSSGPLPVVFDPRVGGSLVGHLLSAMAGSAIARRASFLLDRDGAQLFP
                     EDITIIDEPLRLRGLRSKPFDGEGLPTAPRVLVENGRLTGWLMNAAAARQLGGRPTGH
                     ATRGGGAPGIGATNTHVAASRLSPAELIADIADGVYVTELIGQGVNGVTGDYSRGASG
                     VRIVGGQLAGPVAEFTIAGNLVDMFGQLRAANDLEWYRAVNVPTLRVDGMMIAGD"
     gene            complement(2790..3608)
                     /locus_tag="VN627_08140"
     CDS             complement(2790..3608)
                     /locus_tag="VN627_08140"
                     /inference="COORDINATES: protein motif:HMM:NF021615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HXD03262.1"
                     /translation="MTTARPPFDSNRAWTRATAAIRANGEVLFALAGVFFLLPSLAMT
                     LLFPQPAPLRGGDEAAMMAQAADYYRQTLPVALPMAVLQAAGTLGLLTLLTDRARPTV
                     GQAIRGGFAALLPYLASQIVLGMGLGMLVLIIAGVAGGVGLPWLAIVLAVPIGAVLIT
                     RFALTAPVVAIERWRNPLAALRRSWQLTSGNTWRVLGFFALILLAFAIVMSVVMGLSG
                     IVLALVLPARIAVIVSAVISATLGAGMVILLVACIAATHAQLAGEAADVGTTFD"
     gene            3665..>4098
                     /locus_tag="VN627_08145"
     CDS             3665..>4098
                     /locus_tag="VN627_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006957609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate-protein ligase B"
                     /protein_id="HXD03263.1"
                     /translation="MNALSPSNSPLRPEAWPASIEWRAETGQVAYARALEEMTGRNAA
                     IQEGTARELVWLLEHPPVYTAGTSADPAEMLDPRFEVVRAGRGGRYTYHGPGQRIGYV
                     LLDLTRRARDVKGFVHALEGWVIAALAGFGMEAWRAEGRIGI"
BASE COUNT          604 a         1340 c         1554 g          600 t
ORIGIN      
        1 ttcggccctg gccgggccgg gcgtgttcac agcgtgaagc cttcgccgag atagagccgg
       61 cggacgtttt catcggccac gagcgcttcg gggctgccgg cgaagaggac ctggccgcca
      121 tagatgatgc aggcgcgatc gacgatgtcg agcgtttcgc ggacgttgtg atcggtgatc
      181 agcacgccga tgccgcgctg cttgaggtcg atcaccaggt ggcgaatgtc gctgatcgag
      241 agtggatcga tcccggcgaa gggttcgtcc agcagcatga tcgagggctt ggcggccagc
      301 gcccgggcga tctcgcagcg gcgacgttcg cccccggaga gggccatggc ggcgctggag
      361 cgcaggcggg tgaggccgaa ttcggtgagc aggcgttcca gttcctcggc ccggacgtca
      421 cgatcgggtt cgaccagttc gaggacggag gcgatgttct gttcgaccgt catcccccgg
      481 aagatcgagg tctcctgggg gaggtagccg aggccgagga tcgcgcggcg atacatcggc
      541 aggttggtca catcgatgcc gtccatcagg atgcggccgg aatcgggccg caccaggccc
      601 atgatcgaat agaagcaggt ggtcttcccc gcgccgttag ggccgagcag gccgagcact
      661 tcgcccttgc cgaccgacag ggaaatatcg gtgagcacgg cgcgcttgtc gtagctcttg
      721 gcgatggaga tcacctcgag cccgccttgc gggataggct gcatggcgcc ggcgttggcc
      781 gcgtcggcct gcgggatatc tgcgatgtcg ctcatctggc atggggttta ggcgggggtt
      841 gcggccactt caagcggcca aacaatccgt tttcggcatt tggcaggttt cctgcatggc
      901 tgcggcctgg ctgcaacggc caggtcgcgg cgatgggact tgccagcttg gtttagcggt
      961 gatttactct ccggcaaaac gcctgtcgga gaggaagggc gcaaatggtc ggaatggagc
     1021 gtgaagtgcg cgcagacgcg tccctgccgg attggcgccg gcggatgagc aaccaggtcg
     1081 cctatgccct gctggcatac actgccttgc agatctttct taccgtgggg gcgctccagg
     1141 gggatcgcgg ctcgctgctg ccctatctgg cgctggtcgt gctggtcggc gcgatcattc
     1201 cggcttgccg cgggtgcgaa cgacgctggc aggagttgga agaagggccg gcgcacgacc
     1261 cggccctggc gcgctgctac cgccgcgact gcaaggttct atgggccctc gcgctcggcg
     1321 tgccgctggc gatgaccgcg gtgctcaagg ggctcgcgct ggtcttcgcc ggctgagggg
     1381 agccccgacc gcaacgcatt ttggcgcgag gccctttcgc tggggctcgg ccatcgctag
     1441 atcgcccgga tgctcgatgt ctccgccgcg caggaacgcg ccgccgccct gatcgaccgc
     1501 gcccgccggg ccggcgccga tgccgccgat gcggtctata tcgccgatgc ctcggagagc
     1561 gtggccgtgc gcctgggcaa gctggaggac ctcgagcgct cggagagcga gcacgtgtcg
     1621 ctccgggttt tcgccggcaa gggctcggcc tcgatcggct cgagcgacct tggtgatgcg
     1681 gcgctcgatg agctggccgc ccgcgccgtg gccatggccc gggcggcgcc ggaagattcc
     1741 tatgccgcgc tggcgcccga ggacatgctg gcgcgcgggc ccctgcccga gctcgacctc
     1801 agcgacggcg gcgcggtcga gccggcgcag ctgcgccgga ctgccgagga agtggaagcc
     1861 gctgccctcg ccgtgccggg ggtgagcaac agccagggcg gcagcgccag cgccgggcgc
     1921 acggtcgtcg cgctcgccac cagccatcgc tttgccgggg gctacgagac caccagccac
     1981 ggtgtttccg ccagtgtggt ggcgggcgag ggcgcggcga tgcagcgcga ctatgcctgg
     2041 cggtcggccc ggcaccggac ggacctcccc ggcgcggcgg agattggccg gctcgcgggt
     2101 gagcgggccg tggcgcggct cgctccgggg cgcttgtcca gcgggccgct gccggtggtg
     2161 ttcgatcccc gcgtgggtgg gtcgctggtg ggccacctgc tctcggccat ggccggcagc
     2221 gccatcgccc ggcgggcgag cttcctgctc gatcgcgacg gggcccagct gtttcccgag
     2281 gacatcacca tcatcgacga gccgctgcgg ttacggggcc tcaggtccaa gccgttcgac
     2341 ggcgaaggcc tgccgaccgc cccgcgcgtg ctggtcgaga acggacggct gaccggctgg
     2401 ctgatgaacg ccgcggcggc gcgccagctg ggcgggcggc cgaccggcca tgcgacgcgc
     2461 ggcggcggcg cgcccggaat cggggcgacc aatacccacg tggcggcgag ccggctttcg
     2521 cccgccgagc tgatcgccga cattgccgac ggggtctacg tgaccgagct gatcggccag
     2581 ggggtcaacg gcgtgaccgg cgactacagc cggggtgctt cgggcgtgcg gatcgtgggc
     2641 ggccagctgg ccgggcccgt ggccgagttc acgatcgccg ggaacctcgt cgacatgttc
     2701 gggcagctgc gcgcggccaa tgaccttgag tggtaccgcg cggtgaacgt gccgaccctg
     2761 cgggtcgacg ggatgatgat cgcgggcgac tagtcgaagg tcgtgcccac gtcggccgct
     2821 tcgccggcca gctgggcgtg ggtggccgcg atgcaggcga ccagcaggat caccatgccc
     2881 gcgccgagcg tggcggagat gaccgcggag acgattacgg cgatccgggc cggcaggacc
     2941 agggcaagca cgatgcccga caggcccatc acgaccgaca tgacaatggc gaaggccagc
     3001 aggatcagcg cgaagaagcc gagcacgcgc caggtgttgc ccgaggtgag ctgccacgag
     3061 cggcgcaggg ccgcgagcgg attgcgccag cgctcgatgg cgacgaccgg cgcggtcagc
     3121 gcgaagcggg tgatcagcac ggccccgatg ggcacggcca gcacgatggc gagccacggg
     3181 agcccgactc cgcccgccac tccggcgatg atcaggacca gcatgcccaa gcccatgccg
     3241 agcacgatct ggctggcgag gtagggcagc agggcggcaa agccgccgcg gatcgcctgg
     3301 cccacggtgg gccgggcgcg gtcagtcagc aaggtgagca ggccgagcgt gcccgccgcc
     3361 tggagcacgg ccatcggcag ggcgaccggc agggtctggc ggtaatagtc cgccgcctgg
     3421 gccatcatcg ccgcctcgtc gccgccccgc agcggcgcgg gctgcggaaa cagcagtgtc
     3481 atcgcgaggc tcggcaggag gaaaaacacg ccggcgagcg cgaacagcac ctcgccattg
     3541 gcgcggatcg ctgccgtcgc ccgggtccag gcgcggttgc tgtcgaacgg ggggcgggct
     3601 gtggtcatgc ggcggtgctc atggcccctt gataaccgca ccgaatacgc gcaggcaacg
     3661 cgcgatgaac gccctgtccc cgtcgaattc cccgctgcgg cccgaggcct ggcccgcgag
     3721 catcgaatgg cgcgcggaaa cggggcaggt ggcctatgcg cgggcgctcg aggaaatgac
     3781 cgggcgcaac gccgcgatcc aggaaggcac ggcgcgcgag ctggtctggc tgctggagca
     3841 tccgccggtc tataccgccg ggaccagcgc cgacccggca gaaatgctcg acccgcgctt
     3901 cgaggtggtc cgcgccgggc ggggcgggcg ctatacctac cacgggcccg ggcagcgcat
     3961 cggctatgtc ctgctcgacc tcacgcgccg ggcgcgcgac gtgaagggct tcgtccatgc
     4021 actggagggc tgggtgatcg ccgcgctggc cggctttggc atggaggcct ggcgggccga
     4081 ggggcggatc ggcatctg
//
LOCUS       DATNBV010000341         2367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_836637,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000341 DATNBV010000000
VERSION     DATNBV010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836637"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..408)
                     /locus_tag="VN627_08150"
     CDS             complement(139..408)
                     /locus_tag="VN627_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03264.1"
                     /translation="MGKATFETLDGEEIEINSEDVIHLGQGSEEETTLIELEDGSEVT
                     VVATELEVAGELGLNPLEYIDPEDDDESIEELVDEDDEDDEDSYD"
     gene            complement(505..1107)
                     /locus_tag="VN627_08155"
     CDS             complement(505..1107)
                     /locus_tag="VN627_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03265.1"
                     /translation="MTVRNHLIALLLAGLAAPALAQTQPVPAAKPSDAARPVNERDPS
                     AVDVVATPVTDLNLRKGEIPPALVAAEADPYGLPGGGRCPAMAAEVRRLDAVLGDDVD
                     IAQASRGRVSPGKVAQSVVGSFIPFRGIIREISGANEQQRRLQSAIYAGMARRSFLKG
                     VGLQRGCPWPARPATPQILARIAAQNAAKAQAGSSPKPRR"
     gene            complement(1139..2035)
                     /locus_tag="VN627_08160"
     CDS             complement(1139..2035)
                     /locus_tag="VN627_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HXD03266.1"
                     /translation="MRLDDFDPGSVDVEDQRGGGGFGGLPLGLGGGRIGCGTLVIALI
                     AAAIFGVNPLQLIGGMQGGTDTSQVGGTLPGQQRGGSSASESCTVDQASRESCNALSS
                     LNKTWAPIFQKAGAEFSRPKLVFYSQMGRSGCGAAQSAMGPFYCPTDNGIYLDTDFYR
                     QMSDQLGATGGFARDYVIAHEYGHHIQNLTGVSDQVRQLQERNPGAANQLSVRLELQA
                     DCYAGVWAAKNRDRLEPGDMESGLNAAHQIGDDTLMKNAGRNPSPENFTHGTSAQRME
                     WLRKGMDSGNEDACDTFADLRK"
     gene            2143..>2367
                     /locus_tag="VN627_08165"
     CDS             2143..>2367
                     /locus_tag="VN627_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03267.1"
                     /translation="MFLSGKRALVTGSTSGIGLGIARALHDAGAEVVLNGFGDEAVIA
                     ALCAELDARHVAADLTRREGVETLMGAAGPV"
BASE COUNT          340 a          768 c          831 g          428 t
ORIGIN      
        1 ccttgtgctt aacggcctgc cggcggggac gaccgccctg gcccccgggc gcaaggtgaa
       61 gatcgtggtg tggggggcgg cggcgaagta gccggattgc cgcgtcgtcc cgggacaagt
      121 ccgggacgac gaacaggctc aatcgtagct gtcttcgtcg tcctcgtcgt cttcgtcgac
      181 gagttcctcg atggattcgt cgtcgtcctc cggatcgatg tattcgagcg ggttgaggcc
      241 gagttcaccg gccacctcca gttcggtggc gacgacggtc acctcgctgc cgtcttcgag
      301 ctcgatcagc gtggtctcct cctccgagcc ctgcccgagg tggatcacgt cttcggaatt
      361 gatctcgatc tcttcgccat cgagcgtctc gaacgttgcc ttgcccatac cagcctcctc
      421 gcggccagcg ttcggccaca aggcgctgaa cccctcccgg ggcgaagcgg ttccggggtc
      481 ctgcgacaat ctgttgcaac cggcttagcg acgcggcttg ggtgaacttc cggcttgggc
      541 cttggcggcg ttctgggcgg cgatgcgggc caggatttgc ggggtcgcgg gacgggccgg
      601 ccaggggcag ccgcgctgga gcccgacacc cttgaggaac gagcggcgcg ccatgccggc
      661 atagatggcc gattgcagcc gccgttgttg ctcgttggcg ccggaaatct cgcggatgat
      721 gccgcggaag ggaatgaacg agccgaccac cgactgggcc accttgcccg ggctgacccg
      781 tccgcgcgag gcctgggcaa tgtcgacgtc gtcgcccagc acggcgtcga gccgccgcac
      841 ttccgccgcc atggccgggc agcgcccgcc acctggaagg ccatagggat cggcctccgc
      901 agcaacgagg gcagggggga tttcgccctt gcgcaggttg agatcggtca ccggcgtggc
      961 gacgacgtcg accgcggaag ggtcgcgttc gttgaccggc cgggcagcgt ccgatggctt
     1021 tgcggcgggg accggttggg tctgggccag cgcgggggct gccagcccgg ccagcaacag
     1081 ggcgatcaga tggttgcgaa cggtcatgcg cggggtcccc tgttggtcat ccggttcgtc
     1141 atttcctgag gtcggcgaag gtgtcgcagg cgtcctcgtt gccgctgtcc atgcccttgc
     1201 gcagccattc catgcgctgg gcgctggtgc cgtgcgtgaa gttttccggg ctgggattgc
     1261 ggccggcatt tttcatcagg gtatcgtcgc cgatctggtg ggccgcattg aggccgcttt
     1321 ccatgtcacc gggttcaagc cggtcacggt tcttggctgc ccagacaccg gcatagcaat
     1381 cggcctggag ttcgagccgg acggaaagct ggttggccgc gccgggattg cgctcctgca
     1441 gctggcggac ctgatcggac acgcccgtca ggttctggat gtggtggcca tactcatggg
     1501 cgatgacata gtcgcgcgcg aacccgccgg tggcgccgag ctggtcgctc atctggcggt
     1561 agaaatcggt atcgaggtag atcccgttgt ccgtcgggca gtagaacggc cccattgcgc
     1621 tctgcgccgc gccgcagccg gagcggccca tctgcgaata gaacaccagc ttgggacggc
     1681 tgaactccgc gccggccttc tggaagatcg gcgcccaggt cttgttgagc gaggacaggg
     1741 cattgcagct ttcgcggctg gcctgatcga ccgtacagct ttcgctggcg gaggagccgc
     1801 cgcgctgctg gccgggcagg gtgccgccga cctggctggt atcggtgccg ccctgcatgc
     1861 cgccgatcag ctggagcggg ttcaccccga agatcgccgc ggcgatcagc gcgatcacca
     1921 gcgtgccgca gccgatccgc ccgccgccca agcccagcgg caatccacca aacccgccgc
     1981 cgccgcgctg gtcctcaacg tcgacggagc cggggtcgaa atcgtcaagc cgcatgggtc
     2041 gtccctgtct tgttgcagtg cgtcatgaat ggtggacagg gccgggccca gccgccaagg
     2101 ttccaccgtt catttgccag tatcaaccgg caggagtcga ttatgtttct ctcaggcaag
     2161 cgcgccctcg tcaccggctc gacttcgggc atcggcctgg gcatcgcccg cgccctgcac
     2221 gatgcgggcg ccgaggtggt gctcaacggg tttggcgatg aggcggtgat cgccgcgctc
     2281 tgcgccgaac tcgacgcgcg ccacgtggcc gccgatctca cgcggcgcga aggtgtagag
     2341 acgctgatgg gggccgcggg gccggtc
//
LOCUS       DATNBV010000342         2261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2750214,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000342 DATNBV010000000
VERSION     DATNBV010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2750214"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..464)
                     /locus_tag="VN627_08170"
     CDS             complement(186..464)
                     /locus_tag="VN627_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HXD03268.1"
                     /translation="MLECRRITISGYVQGVFFWGWTVGVAQELGVTGWVRNRSNGTVE
                     VLAMGAPKPFDRFIARMREGAPASRVDAVHIEDATPEDLETFTRRPTV"
     gene            927..1379
                     /locus_tag="VN627_08175"
     CDS             927..1379
                     /locus_tag="VN627_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXD03269.1"
                     /translation="MADHLLSLTWGAEDLPAALGTVASRGADGAFTDPYRAARALALF
                     AAHAARTEAIETVYPAIGDLAGLHAYAEQGQRDGFTGMLALHPAQVPIINAAFTPDSE
                     QVRAARDVVAAFAAAPDAGVLMLGGRMIDAPHLAQARRLLARASATED"
     gene            1662..>2261
                     /locus_tag="VN627_08180"
     CDS             1662..>2261
                     /locus_tag="VN627_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HXD03270.1"
                     /translation="MEEIRIRKITLRDLRHALALGWDDFMDKRGDLVFVGLIYPLAVL
                     LGAFYVLHVSILPLIFPIMTGSILFGPAAATGFYELARRREQGLDARWRHFLDVLRGP
                     SAEPLFALTSVIAGLFVLWLIAAWYINLKTMGLDTPHTPLVFLQAVLSTPAGWTMIVV
                     GNLVGLGFAITVLAIGVVSFPMLVDRPVSWTVALRTSVRV"
BASE COUNT          356 a          727 c          708 g          470 t
ORIGIN      
        1 tctgcccgtt gttcggtgag gaagcggttc agttttcggg cgatggacat gatgtttctc
       61 caacgggtat ggtgattcgc caaccttcgc cgcggagcag cgactgtccg cggagggcgt
      121 cgccagatgc agcactatac ccggttttga gtcgattctg aagttcatcc cgggcaatct
      181 gagcctcaga ccgtcggacg ccgtgtaaag gtttcgaggt cctcgggcgt ggcatcctcg
      241 atgtgcacgg catcaacccg cgaggccggt gccccctctc gcatccgcgc gatgaaccgg
      301 tcgaacggtt tgggcgcgcc catcgccaga acctcaaccg tgccgttgga gcgattacgt
      361 acccagccgg tcacaccaag ttcctgcgcc actccaaccg tccaccccca gaagaacacg
      421 ccctgaacat agcccgatat cgtgatgcga cggcattcca acactgctgg atccattcct
      481 tggcgtgacc gaatgcatag cgtactcgcc acttgttcaa ttcaggatgg ccgctccgct
      541 tcatcgccgt gctcccactg atatagcgga tgagtggcct acttccagcg ccgtgcggtg
      601 tccggtaaat cctccgcctg gcatgagcgg caatgtttgg tcctgggcca gcctctcccg
      661 caaggcgcag gcaagggatc cgatagcggc ttttttggcc cagccatgtc cggtccggcg
      721 gctggtccgg gtcaatggac agggcagcgg cctgctcgcg gacgacctgc cgtgcgtcaa
      781 cggggccgaa ggggtcgtgc tgcccaaggc cgaaggcgcc gccagcattc ggaaggtagc
      841 ggccagactg cccgtgccct tgccgatcct tccgaccgcc acggaaaccc cctgctacga
      901 tcttcgtcct cggcacctat cgagaagtgg cagaccatct cctcagcctg acctggggcg
      961 ccgaggacct gccggctgcc cttggcaccg tcgccagccg cggggccgat ggcgccttta
     1021 ccgatcccta tcgggccgcc cgggccctgg cactgttcgc ggcccatgcg gcgaggacgg
     1081 aggcgatcga gaccgtttac cctgcgattg gggatctggc cggtctgcat gcctatgccg
     1141 aacagggcca gcgcgacggc tttaccggga tgctggccct gcatcccgcg caggtgccaa
     1201 tcatcaacgc tgcgttcacg cccgacagcg agcaggtgcg cgccgcacgc gacgtggtgg
     1261 ccgccttcgc ggccgcaccc gatgccgggg tgctgatgct gggcggccgg atgattgatg
     1321 caccgcatct cgctcaggcg cgccggctgc tggcgcgggc gagcgcaacg gaagattgat
     1381 cgggaaatcg gtaactgcgc tccgttccgg ccgccatcct gacgatggcc catccttccg
     1441 acggcgtctt tccgtttccg atctgaaggc gtattatact cccggtacga cgaggtgctc
     1501 ctccgttgga cactgtcagt cgttgaccga gtgcgcggga aaagccgacg ctcttgagcc
     1561 ataccgggtt tgagtatggc gcggcggaag gccagctccc gggagatcag gatggagagc
     1621 gactgatggc aatagctcac ccgcctttcg tggccccggc catggaggaa attcgtattc
     1681 gcaagatcac cctgcgcgat ctgcgccatg cccttgccct ggggtgggat gatttcatgg
     1741 acaagcgggg cgatctcgtt ttcgtgggcc tgatctaccc gctcgcggta ttgttgggcg
     1801 cgttttatgt cctgcatgtt tcgatcttgc cgttgatctt tccgatcatg accggatcga
     1861 tcctgttcgg acctgccgcg gcgaccggat tttacgaact cgcccgacgg cgtgagcagg
     1921 ggcttgatgc ccgctggcgc cattttctcg acgtgttgcg cgggccttcc gctgaaccgc
     1981 tgttcgcact gacaagcgtt atcgctggcc tgttcgtcct gtggctcatc gccgcgtggt
     2041 acatcaatct caagacgatg gggctcgata cgccgcacac gccgcttgtg ttcctgcagg
     2101 ctgtcctctc cacgccggcg ggctggacca tgatcgtcgt gggcaacctc gtggggctcg
     2161 gctttgcgat cacggtgctg gcaatcggcg tcgtatcgtt tccgatgttg gtcgatcgtc
     2221 ctgtgtcatg gacggttgcc ctgcggacgt ccgtgcgtgt c
//
LOCUS       DATNBV010000343         6057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1782851,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000343 DATNBV010000000
VERSION     DATNBV010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782851"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VN627_08185"
     CDS             complement(<1..764)
                     /locus_tag="VN627_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXD03271.1"
                     /translation="MSAIRESLVRIMTILKEFANGKDDPGDGLNVLRLSFVSVVGALL
                     AAFVLIVRPFIWPGSSDEWHRTVAILIAYALCVIIAGLRTGNRIRATQALAHASAGAE
                     RVKTQELFVMTDMLQSADTNEDALAVLRATSLRLLPQFGAALYVFNNSRDRLDLIGSW
                     ALPEDIALPQLLSPGNCWALKRGKFHINDAPSGTLCCSHHGAELSTIEIPMLARGAVY
                     GLLMFATDREGAFDELRNVQRLGRMLADSMSLALAN"
     gene            887..1681
                     /locus_tag="VN627_08190"
     CDS             887..1681
                     /locus_tag="VN627_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HXD03272.1"
                     /translation="MKHSPLKLAILATATAAISLTAGCGGKGGKNRDTAYVARDVDTL
                     YLAAKDRLDRGRSKEAAALFDEVERQHPYSPWARRAQLMSAFSYYVGRDYIKAISSAQ
                     RFLSIHPGNKDAAYAYYLIALSYYEQISDVTRDQKATAQALAAMTELTRRYPDTRYAA
                     DARLKLDLINDHLAGKEMDIGRAYERQGKWLAASIRFRAVIEKYQTTSHTPEALYRLA
                     ESYLSIGLPEEAQKAAAVLGKNYPGNEWYGRAFKLMNKNAPGVLAS"
     gene            1875..2336
                     /locus_tag="VN627_08195"
     CDS             1875..2336
                     /locus_tag="VN627_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXD03273.1"
                     /translation="MLSQKTRYTIRALQHLADQWRPDRSGGAVRLDAIAEAQNIPRKF
                     LTVILSELVREGIVISHRGREGGYELALPPVDIRYGDIIRLTRGSLALVPCAARNAHE
                     KCRNCLPEAECRLRGLMLTLRDEMAAMLDRVSLADPIKLEPPFEESDQDSA"
     gene            2426..2617
                     /locus_tag="VN627_08200"
     CDS             2426..2617
                     /locus_tag="VN627_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HXD03274.1"
                     /translation="MPAPPHSRSADDRRPPLSPEEAAAAAISAVAEAVSRLRYGAIGL
                     TVHDGRVVQLEVTERQRFT"
     gene            2707..5229
                     /locus_tag="VN627_08205"
     CDS             2707..5229
                     /locus_tag="VN627_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXD03275.1"
                     /translation="MHSFVRGGLLAATALSFGALSAPAFAADAAAEAAATAEASSATG
                     AGSATPQVTDDDPSRGEVIIVTARRRQETAQAVPLAISVIKADSIESTGNFNVVKLQQ
                     VAPTLQVYSSNPRNTSVNIRGLGVPFGLTSDGFEQGVGIYVDDVYNSRVAAATFDFLD
                     VQQVEVLRGPQGTLYGKNTTAGAINITTNQPTFDFEGRAELTLGNLNLKQAKAAISGP
                     LSDTVAARIAIASTSRRGTIYNVATKRWINEQDNQGIRAQLLFQPSDDLSITLSGDYS
                     KQDPYGFGTSFVRVGTTQRLITRQYDALVAAVNAANPGRNYAVPSRNVYDRLTDIDAP
                     LQAGNKIGGASLRVKWKLGAGTLTSITAWRFWDWKPQNDRDFSGLSIVSASNNPSQQN
                     QYSQEFRYNYSGDRLDFVVGLFGFKQRIDTQGLEQQGADASRWSLTGSQAGDPTILGG
                     LTAQNTQYLKSESAALYGQLSYKLTDQLTIQPGLRLNYDKKEGYYNRVVTTGAGTVIS
                     CTPAPAAGTTLAAQCGIYQPQTTSPEASKWNVSYDLNVNYKVAHDVLVYATYAKSFKT
                     LGINQNGLPLTTANVPDLTASTVKPESVNHFEVGIKAQFWNRRATFNLAAYRTEIKDF
                     QATVNGGQFGTVRGYLANAEKVRSQGIEADFKIRPSDRLTAYINGAYTDAKYVSFKNA
                     PCPPELSGGTFTTDPTKVSPAGQAGTSAKPSFSPDKCDISGQALPGVSKWSFSYGAEY
                     NIPAKLLGKDGQAYIGVDGNYRSDWNSNASPSVYTKVDGYALTNFRAGFRGDGFDVFG
                     WVRNAFNVNYIELLQVAPGNTGLIAGTAGDQRTWGGTIKFSF"
     gene            complement(5445..>6057)
                     /locus_tag="VN627_08210"
     CDS             complement(5445..>6057)
                     /locus_tag="VN627_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HXD03276.1"
                     /translation="SSIERLAVEIHHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLT
                     ENLTGLARMVRSAVTPALENVALWHERDISHSSVERFIGPDATITLDFALARLTSVVD
                     KLVVYPERMEKNLNKMGGLVHSQRVLLALTQAGLERDAAYRLVQRNAMKVWESDGQLS
                     LLELLKADPEVAAALTPAQIEEKFNLDYHFKAIDTIFQRVFGE"
BASE COUNT         1149 a         1951 c         1935 g         1022 t
ORIGIN      
        1 atgttggcca gggccagcga catcgaatcg gcgagcatcc ggcccagccg ctggacattg
       61 cgcaattcgt cgaacgcgcc ctcgcgatcg gtggcgaaca tcagcaagcc gtagaccgcg
      121 ccgcgcgcca gcatcgggat ctcgatggtc gagagttccg cgccatggtg gctgcaacac
      181 aacgtgccgc ttggcgcgtc gttgatgtgg aacttgccgc gcttgagcgc ccagcaattg
      241 cccggggaga gcagctgggg aagggcaatg tcctcgggca gggcccagga accgatcagg
      301 tcgagccggt cgcgcgaatt gttgaacaca tagagcgcgg cgccgaattg cggcaggagg
      361 cgcagcgagg tggcgcgcag gacggccagg gcatcctcat tggtatccgc gctctggagc
      421 atgtccgtca tcacgaacag ctcctgggtc ttgacgcgtt ccgctccggc cgaggcatgg
      481 gccagggcct gggttgcgcg aatccggttg cccgtgcgca ggcctgcaat gatgacgcag
      541 agtgcgtagg cgatcaggat ggcaaccgtg cggtgccatt cgtcggagct gccaggccag
      601 atgaacgggc gcacgatcag gacaaatgcc gccaggagtg caccgacaac cgagacgaag
      661 ctcagccgca ggacgttgag cccgtcgccg gggtcgtcct tgccgttcgc aaactccttg
      721 aggattgtca ttattcgcac gaggctctcc cggatcgcgc tcatttaagg acaagaggtt
      781 gccgacgggt aaatgtcagg ccctcggtag ttgcgcttcc atcgcgtgcg gcggcaccgg
      841 cgctttcact tggcgttcat gcccgcgctg gttatagcgc ctgcccatga agcattcgcc
      901 gctcaagctc gccatcctcg ccactgccac tgccgcgatt tcgctgaccg ccggatgcgg
      961 cggcaagggc ggcaagaacc gcgacacggc ctatgtcgcc cgcgacgtgg acacgctgta
     1021 cctggcggcg aaggaccggc ttgatcgggg ccgctccaag gaagcggccg ccctgttcga
     1081 cgaggtggag cgccagcacc cctattcgcc ctgggcccgc cgtgcccagc tgatgagcgc
     1141 cttcagctac tatgtcggtc gtgactacat caaggcgatc tcatcggcgc agcgcttcct
     1201 ctcgatccac ccgggcaaca aggacgcggc ttacgcctac tacctgatcg ccctgtccta
     1261 ttacgagcag atcagcgacg tgacgcgcga ccagaaggcg acggcgcagg cgctcgcggc
     1321 gatgaccgag ctgacccggc gctatcccga tacccgctac gccgccgacg ctcgcctgaa
     1381 gctcgacctc atcaacgatc acctcgccgg caaggaaatg gacattggcc gggcttacga
     1441 gcggcagggc aagtggctcg ctgcctccat ccgcttccgc gcggtgatcg aaaagtacca
     1501 gacgaccagc cacacccccg aggcgctcta tcgcctggcg gagagttatc tctcgatcgg
     1561 cctgcccgaa gaggcacaaa aggctgcggc cgtgcttggc aagaactacc cgggcaacga
     1621 gtggtatggc cgcgcgttca agctgatgaa caagaacgcg ccgggagtat tggccagctg
     1681 atcaaggcag gccttgtccc ggtctgatca cgggccgatg tgattcaact gccgacgttg
     1741 tccgcaacca tcgcgacggt tcggatggaa cagatgcccc cagattcccc ggcctgtacc
     1801 aggtgggacg atccaggcag gccaggtcta ccggtgaggt tgactttacc cgggcaggct
     1861 agtcttctca ggtcatgctc tcacagaaga cgcgctacac catccgggcc ctgcagcacc
     1921 ttgccgatca atggcgaccg gaccgttcgg gcggcgcggt tcggctggat gccattgcgg
     1981 aagcccagaa cattccgcgc aagttcctca ccgtgatcct gtccgaactg gtgcgcgaag
     2041 ggatcgtgat ctcgcaccgc ggccgggaag gcggctatga gctggccctc ccgccggtcg
     2101 acatccgcta cggcgacatc atccggctga cccggggtag cctggccctg gtgccttgtg
     2161 cggcgcgcaa tgcccatgag aagtgccgca attgcctgcc ggaggcggaa tgccggctgc
     2221 gcgggctgat gctgaccctg cgcgacgaaa tggccgcgat gctcgatcgg gtcagcctgg
     2281 ccgatccgat caagctggaa ccgccgttcg aggagagcga tcaggatagc gcgtgaaagc
     2341 cgcgctttat cgccggatag gtcttctcct attgtctatc gattaggtag agataagaac
     2401 tgcctccacc aaggaggcac cacctatgcc agcaccacca cattcgcgca gcgctgacga
     2461 ccggcgcccg cccctttcgc ccgaggaagc tgcggcggcc gcgatcagcg cggttgccga
     2521 ggcagtctcg cggctgcgct acggcgccat cggcctgacc gtccacgacg ggcgggtcgt
     2581 ccagctggaa gtcaccgaac gccagcgctt cacctgaacc ccgcattcgg gggcaaaatt
     2641 cacggaaaat accaaggtgc agaccggacc accggatgca tccattccct acgcaaggat
     2701 tggatcatgc attcgttcgt ccgcggcggc ctgttggccg caaccgccct ctcgtttggc
     2761 gccctgagcg cgcccgcctt tgctgccgat gcggccgccg aggctgccgc aactgcggaa
     2821 gcctcaagcg ccactggcgc cggctctgcc acaccccagg taaccgatga cgatcccagc
     2881 cggggcgagg tgatcatcgt caccgcccgc cgccgccagg aaactgccca ggccgtgccg
     2941 ctggcgatct cggtgatcaa ggcggactcg atcgaatcga ccggcaactt caacgtggtg
     3001 aagctccagc aggtggcgcc aaccctgcag gtctattcct cgaacccgcg caacacctcg
     3061 gtcaatatcc gcgggctcgg cgtgccgttc ggcctcacca gtgacggctt cgagcagggc
     3121 gtgggcatct atgttgacga cgtctacaac tcgcgcgttg ccgctgcgac cttcgacttc
     3181 ctcgacgtcc agcaggttga agtgctgagg gggccccagg gcacgctcta tggcaagaac
     3241 accacagccg gcgcgatcaa tatcaccacc aaccagccga ccttcgactt cgaggggcgg
     3301 gccgaactga cccttggcaa cctcaacctg aagcaggcca aggcggcgat ctccgggccg
     3361 ttgagcgaca cggtggccgc ccgcatcgcc attgcctcga ccagccggcg cggcacgatc
     3421 tacaacgtgg cgaccaagcg ctggatcaac gagcaggaca accagggcat tcgcgcccag
     3481 ctgctgttcc agcccagcga cgatctatcg atcaccctgt cgggagacta ttcgaagcag
     3541 gacccctacg gcttcggcac ttcgttcgtg cgggtgggga ctacccagcg cctgatcacg
     3601 cggcagtacg atgcgctggt ggcggcggtg aatgcggcta atccggggcg caactatgcc
     3661 gtgccgagcc gcaacgttta tgaccggctg actgatatcg atgcaccgct gcaggccggc
     3721 aacaagatcg gcggcgcttc gctgcgggtg aagtggaagc tgggcgcggg cacgctgacc
     3781 tcgatcacgg catggcggtt ctgggattgg aagccgcaga atgaccgcga tttctcgggg
     3841 ctgtcgatcg tctccgcttc gaacaaccca tcgcagcaga accagtacag ccaggagttt
     3901 cgctacaact actcgggcga caggctcgat ttcgtggtgg gcctgtttgg gttcaagcaa
     3961 aggatcgaca cgcaggggct ggagcagcag ggagcggacg ccagccgctg gagcctgacc
     4021 ggcagccaag cgggcgatcc gaccatcctc ggcgggctga ctgcccagaa tacccagtat
     4081 ctcaagagcg agagcgctgc gctctacggt cagctgagct acaagctgac cgaccagctg
     4141 acgatccagc cgggcctgcg cctgaactac gacaagaagg aaggctatta taaccgggtc
     4201 gtgacgaccg gtgcgggcac ggtgatcagt tgcaccccgg cgccagctgc gggcacgacg
     4261 ttggccgccc agtgcggcat ctaccagccg cagactactt cgcccgaagc aagcaagtgg
     4321 aacgtcagct acgacctcaa tgtcaactac aaggtcgccc acgacgtgct ggtctatgcg
     4381 acctatgcca agagcttcaa gacgctgggc atcaaccaga acggcctgcc gctgaccacg
     4441 gcgaacgtgc ctgacctgac cgccagcacg gtgaagccgg aatcggtgaa ccacttcgag
     4501 gtgggcatca aggcgcagtt ctggaaccgc cgggctacct tcaacctcgc ggcctaccgg
     4561 accgagatca aggacttcca ggcgacggtc aacggcgggc agttcggcac ggtccggggt
     4621 tatctcgcca acgccgagaa ggttcgttca caaggcatcg aggccgactt caagatccgc
     4681 ccgagcgacc gtctgacagc ctacatcaac ggcgcctata ccgatgccaa gtacgtcagc
     4741 ttcaagaacg cgccatgccc gccagagctt tcggggggta ccttcaccac cgaccccacg
     4801 aaggtgagcc ccgcggggca agcagggacc tcggccaaac cgtcattcag ccccgacaag
     4861 tgtgacattt cgggccaggc cttgccgggc gtgtcgaagt ggtcgttctc ctatggcgcg
     4921 gaatacaaca tcccggccaa gctgctgggc aaggacggcc aggcctacat cggcgttgac
     4981 gggaactacc ggtcggactg gaactcgaat gcatcgccct cggtctatac caaggttgac
     5041 ggttatgccc tgaccaactt ccgcgccggc ttccgcggcg atgggttcga tgtgttcggc
     5101 tgggtgcgca atgccttcaa cgtgaactac atcgagctgc tgcaggtggc gcccggcaat
     5161 accggcctga tcgccggcac ggctggtgac cagcggacct gggggggcac gatcaagttc
     5221 tcgttctgag gctcggttat cccgatgcaa aggcccgcgg gagtgatccc gcgggccttt
     5281 tgttttccgg agcgtaccag cctgcagacg cggtcatgcc gaacttgttt tcagcaccca
     5341 tggcgcggac cttgcggaaa gcgccgcgcc agaaaggagg cgccgtgccg gcggatggca
     5401 ggtgccatgg gtcctgaaac gagttcagga cgacggggaa aaggctattc cccgaacacc
     5461 cgctggaaga tcgtgtcgat cgccttgaag tggtagtcga ggttgaactt ctcctcgatc
     5521 tgcgcgggcg tgagcgcggc ggcgacttcc ggatcggcct tcagcagttc gagcagcgag
     5581 agctggccat ccgattccca taccttcatc gcgttgcgct ggaccaggcg gtaggccgca
     5641 tcgcgttcca gcccggcttg cgtcagggcg aggaggacgc gctgggagtg aaccaggccg
     5701 cccatcttgt tgaggttctt ttccatccgc tcaggataga ccaccagctt gtcgaccacc
     5761 gaggtcagtc gggcgagggc gaagtcgagc gtgatcgttg cgtcggggcc aatgaagcgc
     5821 tccaccgagg agtgcgagat gtcgcgctca tgccacaggg ccacgttctc cagcgccggg
     5881 gtgacggcgg agcggaccat gcgggcgagg ccggtgaggt tttcggtgag caccgggttg
     5941 cgcttgtgcg gcatggcgct ggagcccttc tggcccggcg agaagtactc ctcggcctca
     6001 agcacctcgg tgcgctgaag gtggtggatt tccaccgcga ggcgctcgat cgagctg
//
LOCUS       DATNBV010000344         4184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3035861,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000344 DATNBV010000000
VERSION     DATNBV010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3035861"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VN627_08215"
     CDS             complement(<1..515)
                     /locus_tag="VN627_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HXD03277.1"
                     /translation="MSTRQASDEHDQVIESRDQLVAPMAAGEKPRERWRVGTEHEKLV
                     YRTADFRAPSYDEPGGIRDLLLSLREFGWEPIEEGGKVIAMGGADGTVSLEPAGQLEL
                     SGAPLATIHETCAEFDAHLHELKGLSEELGIIWLSLGFHPFATHADLPHVPKLRYGVM
                     EKYLPTRGPRAL"
     gene            complement(555..1316)
                     /locus_tag="VN627_08220"
     CDS             complement(555..1316)
                     /locus_tag="VN627_08220"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HXD03278.1"
                     /translation="MTAQHHPMTPAWPPKSAPRLFVHVPLTDGVPVLIEGPQAHYLAR
                     VMRVGAGDAVILCDDLTGEWACRVREAGKREVVVVPEVHSRPREAVPDFWLVAALLKK
                     DRFDAVLEKATELGVAKIQPVITRRCVADKLNGERARTIVVEAAEQCARTALPELELV
                     VKLDLLLRDWNPHRTLFFADENGGEPAAAAFAAHSGPAALLTGPEGGFDDTERAAIRA
                     LPCAVPISLGPRILRGETAAISGVGVWMAVAGDWK"
     gene            1363..2265
                     /gene="ubiA"
                     /locus_tag="VN627_08225"
     CDS             1363..2265
                     /gene="ubiA"
                     /locus_tag="VN627_08225"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HXD03279.1"
                     /translation="MTAEIVPDSEHRGIIARLPQVPRDLAQLARLDRPIGWWLLFWPC
                     AWGVLLGGGAARWDLVLWLLVGAVAMRGAGCVYNDLVDADLDRQVARTAARPVASGRI
                     STKLAWAWLLLLCGIGLAVLLQLRFTAQLVALCSVLPVAAYPFMKRITWWPQAWLGLV
                     FSWGALVGWVEVRGDYLPVLAALYAGSICWVIGYDTIYALQDREDDALVGIRSSARAM
                     GSHVTAGVAMFYSAAILLWSLAFWLLRPDWVALVALIPVALHLLWQVATLHEADGDNA
                     LARFRSNRLAGLLMALACWVVGNA"
     gene            complement(2262..3488)
                     /locus_tag="VN627_08230"
     CDS             complement(2262..3488)
                     /locus_tag="VN627_08230"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HXD03280.1"
                     /translation="MSGSAIWHPFTQHGLGEPIPLIERADGAFLHGADGSRWIDAISS
                     WWVTTHGHCHPRIMAAIAGQAQQLDQLIFAGWTHEPAESLAAGLRAIMPETLTRVFFS
                     DSGSTSVEVALKMALGFWRNRGIDRHRIVVMEHSYHGDTIGAMSVGARGVFNRAYEPL
                     LFDVDTVPFPASGGEQATFDALEAACRAGAAAFIVEPLVLGAGGMLVYPPAVLAEMRR
                     ICAAHEVLFIADEVMTGWGRTGTLLACEQAGIVPDLLCLSKGLTGGAMPLAVTMASEA
                     IWEAHFSADRGQMFFHSSSYTANPIACAAANANLAVWREEPVAERIATLSARQAVRLD
                     RLGNVENPRLCGTIAAADLPGGAGYLADVAPRLMAAAREAGVLLRPLGNTVYVMPPYC
                     IDEAALDAVYEVISGF"
     gene            complement(3485..4114)
                     /gene="bioD"
                     /locus_tag="VN627_08235"
     CDS             complement(3485..4114)
                     /gene="bioD"
                     /locus_tag="VN627_08235"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HXD03281.1"
                     /translation="MSAIVITGTDTGVGKTLFAAALTGALGAYYWKPVQSGPDDEGGS
                     DSERVARLAGIPAAQVLPEVYRLAEPLSPHRAAELDGVAIDPARLVLPEVAGALVVEG
                     AGGVLVPLTREVLYGDVFARWRAPVVIVARTGLGTINHTLLSLEALRARGVPVLGVAF
                     SGEANDDNEATICAMGRVKRLGRLPRLVEVSPQTLAEAFAANFRLKDFA"
BASE COUNT          633 a         1529 c         1356 g          666 t
ORIGIN      
        1 agcgcgcgcg ggccgcgtgt cggcaggtac ttttccatca cgccgtagcg aagcttcggc
       61 acgtgcggca gatcggcgtg cgtcgcgaac gggtggaaac cgaggctcag ccagatgatg
      121 ccgagctctt cgctgaggcc cttgagctcg tgcaggtgcg cgtcgaactc ggcgcaggtc
      181 tcgtggatcg tcgcgagcgg cgcgcccgac agctcgagct ggccggccgg ctcgaggctg
      241 accgtgccat cggctccgcc catggcgatg acctttccgc cctcctcgat cggctcccag
      301 ccgaactcgc gtaagctcag cagcagatcg cggatgccgc cgggctcgtc ataggagggc
      361 gcgcggaaat cggcggtgcg gtagaccagc ttctcgtgct cggtcccgac ccgccagcgc
      421 tcgcgcggct tttcccccgc cgccatgggc gcaaccagct ggtcgcggga ttcgataacc
      481 tgatcgtgct cgtcgcttgc ctgccgcgta ctcatggcgc gcgcgttaag tcagcagcaa
      541 ggttcgcgcc acatttattt ccagtcccct gccacggcca tccacacccc gacgccggaa
      601 atcgccgccg tctccccccg caagatgcgt gggcccagcg agatcggcac ggcgcaggga
      661 agggcgcgga tcgccgcccg ctcggtgtcg tcaaatccgc cttccgggcc ggtgagcagg
      721 gctgccggcc cggaatgggc ggcgaaggcg gcggcggcgg gttcgccccc gttttcgtcg
      781 gcaaagaaca gggtgcgatg cgggttccag tcgcgcagca gcaggtccag cttcaccacc
      841 agctccagct cgggcaatgc cgtgcgcgcg cattgttcgg cggcttcaac cacgatcgtc
      901 cgcgcccgct cgccattgag cttgtcggcc acgcagcgcc gggtgatcac cggctggatc
      961 ttcgccaccc ccagctcggt cgccttttcc agcaccgcgt cgaaccggtc cttcttgagc
     1021 agggccgcca ccagccagaa atcgggcact gcctcgcgcg gccgcgaatg gacctcgggc
     1081 accaccacca cttcgcgctt gcccgcctcg cgaacgcggc aggcccattc gccggtcagg
     1141 tcatcgcaca ggatcaccgc atcgcccgcg cccacccgca tgacccgcgc caggtaatgt
     1201 gcctggggcc cctcgatcag caccggcacc ccatctgtca gcggcacgtg cacgaacagg
     1261 cgcggcgcgc tcttcggcgg ccaggcgggg gtcatcgggt ggtgctgcgc ggtcatgccc
     1321 tgcagcctag ccgatggcgc gccggcccgc tatgccgcgc gcatgactgc cgaaatcgtc
     1381 cccgacagcg aacaccgcgg gataatcgcc cgcctgccgc aggtcccgcg cgacctcgct
     1441 cagctggcgc ggctggatcg gccgatcggc tggtggctgc tgttctggcc ctgcgcctgg
     1501 ggcgtgctgc tcggcggcgg ggcggcacgg tgggatctgg tcctctggct gctggtcggc
     1561 gcggtggcca tgcgcggggc cgggtgcgtc tacaacgacc tcgtcgatgc cgatctcgac
     1621 cggcaggtgg cgcgcaccgc cgcccgtccg gtcgccagcg ggcggatttc gacgaagctt
     1681 gcctgggcct ggctgctgct gctgtgcggc attggccttg cagtgctgtt gcagttgcgg
     1741 ttcacggccc agctggtggc gctctgctcg gtcctgccgg tcgccgccta ccccttcatg
     1801 aagcgcatca cctggtggcc ccaggcttgg ctgggcctcg tcttctcatg gggcgcactg
     1861 gtcggctggg ttgaagttcg gggcgactat ctgcctgttc tggcagcgct ttatgccgga
     1921 tcgatctgct gggtgatcgg ctatgacacg atctacgccc tgcaagaccg cgaggatgat
     1981 gccctcgtcg gcatccgctc cagcgcgagg gccatgggct cccacgtgac cgcaggggtc
     2041 gccatgttct actccgccgc tatcttgttg tggtcattgg ctttctggct cctgcgccct
     2101 gactgggtag cgctcgtcgc gctcattccc gtggccctcc acctgctctg gcaagtcgcc
     2161 accctgcacg aagccgatgg cgacaacgcc ctcgcccgct tccgctccaa ccgcctcgcc
     2221 ggcctgctca tggcgctcgc ctgctgggtg gtcggaaacg cctaaaaccc ggaaatcacc
     2281 tcataaactg catccaacgc agcctcgtcg atgcaatagg gcggcattac gtaaaccgtg
     2341 ttcccgagcg gccgcagcag caccccggcc tcgcgcgctg ccgccatcag gcgcggagcc
     2401 acgtcggcca ggtagcccgc gccacccggc aggtccgcgg cggcaatcgt cccgcacagc
     2461 cgcggatttt cgacatttcc aaggcgatcc aggcgcaccg cctgccgcgc tgacaatgtc
     2521 gcgatccgct ccgccaccgg ctcctcgcgc cacacggcga ggttcgcatt ggctgccgca
     2581 caggcaatcg ggttggcggt gtagctcgag gaatggaaga acatctgccc ccggtcggcg
     2641 gagaaatgcg cctcccagat cgcctcgctg gccatggtca ccgccagcgg catggccccg
     2701 ccggtgaggc ccttggacag gcacagcagg tccgggacga tcccagcctg ctcgcaggcc
     2761 agcagcgtgc cggtgcggcc ccagccggtc atcacctcgt cggcaatgaa cagcacctcg
     2821 tgcgccgcgc agatccgccg catctccgcc agaacggcgg gcggatagac cagcattccc
     2881 cccgcgccga gcaccagcgg ctcgacgatg aatgccgccg cgcccgcccg gcaggccgcc
     2941 tccagggcat cgaaagtcgc ctgctccccg ccacttgccg ggaagggcac ggtgtcgaca
     3001 tcgaacagca gcggctcgta agcccggttg aatacccccc gcgctccgac cgacatcgcc
     3061 ccaatggtat ccccgtggta ggaatgctcc attaccacga tccggtgccg gtcgatcccg
     3121 cgattccgcc agaagccgag cgccatcttg agcgccactt ccacgctggt cgaaccggaa
     3181 tcagagaaga acacgcgagt cagcgtctcg ggcatgatcg cccgcaggcc cgcggccagt
     3241 gactcggcgg gctcgtgagt ccatccggcg aagatcagct ggtccagctg ctgcgcctgc
     3301 ccggcaatcg cggccatgat gcgcgggtgg caatggccgt gggttgtcac ccaccatgag
     3361 gagatagcat cgatccaccg gctgccgtcg gcgccatgaa gaaaggcgcc gtccgcccgc
     3421 tcgatcagcg ggatcggctc gcccaggccg tgctgggtga agggatgcca gatggccgag
     3481 ccgctcatgc gaaatctttc aagcggaagt tcgcggcaaa ggcctcggca agggtctgcg
     3541 ggcttacctc gacaagacgc ggcagacggc ccagccgctt tacccggccc atggcgcaga
     3601 tcgtcgcctc gttgtcatcg ttggcctcgc ccgagaaggc cacgcccagc accggcaccc
     3661 cgcgcgctct caaggcctcc agcgacagca atgtgtgatt gatcgtgcca agcccggtgc
     3721 gcgcgacgat caccaccggc gcgcgccagc gggcgaagac atccccatac agcacctcgc
     3781 gcgtcagcgg caccaacact ccgcccgcgc cctcgacgac cagcgccccg gcaacctcgg
     3841 gcaggaccag tcgggcagga tcgatcgcca ccccgtccag ctcggcggcc cggtgcgggg
     3901 agagcggctc ggccagccga tagacctcgg gcaagacctg tgcggcggga atgcccgcca
     3961 gccgcgccac ccgttcgctg tcgctcccgc cctcgtcgtc tgggccggac tgcaccggct
     4021 tccagtaata cgcgccaagc gccccggtca gcgcggcggc gaacagggtc ttgccgacgc
     4081 cggtatcggt tccggtgatc acgatcgcgc tcatgcggca tccctgcgca gcaggcaggt
     4141 ggcgacttcg taggtggcga cggcgcctcc cgcgtcgaac cggg
//
LOCUS       DATNBV010000345         3448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1482251,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000345 DATNBV010000000
VERSION     DATNBV010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482251"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VN627_08240"
     CDS             complement(<1..816)
                     /locus_tag="VN627_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016510713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HXD03282.1"
                     /translation="MDPRSIAAATGIRLHFCDDRQPGITRKRLGRGGRVAWAYYDPAG
                     QRITDRAEIDRLNRIALPPAYEQAWFCPHGDGHLLATGIDARGRKQYRYHPLFRLTRE
                     SEKFDHTARFGSLLPRVRKRVEQDLSVRALCRTRAIASVVRLLDTGGIRVGNEAYARA
                     NRSFGATTLRMRHVEVRPRALKLRFTAKSGKLREMTITDRSLLRFVRAMQDLPGQHLF
                     QYVDEAGAPCPVSSGDVNDYLRETMGEAFTAKHFRTWHASAMAFELLATATGKL"
     gene            900..1739
                     /gene="yghU"
                     /locus_tag="VN627_08245"
     CDS             900..1739
                     /gene="yghU"
                     /locus_tag="VN627_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HXD03283.1"
                     /translation="MSDDYTPPVVWEAPAPADGMGALFSFNRAVSGPLRDEELPVGRH
                     PLQLYSLATPNGQKVGIMLEELLAAGHAGAEYDAWLINISQGDQFTSGFVDINPNSKI
                     PALVDRSGPEPIRVFESGAILLHLAEKFGAFLPREGAARAETLSWLMWQMGTAPFIGG
                     GFGHFYAYAPTKIEYAIDRYTMETKRLLHVADTRLAQCRYLAGDEYTIADMAVLPWFA
                     GLWRGTVYNEARTFLSMDDYAHLGRWVEELLARPAVARGRKVTSLEMPERHSAADFDA
                     IVL"
     gene            1785..1859
                     /locus_tag="VN627_08250"
     tRNA            1785..1859
                     /locus_tag="VN627_08250"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1817..1819,aa:Gln,seq:ttg)
     gene            2446..>3448
                     /locus_tag="VN627_08255"
     CDS             2446..>3448
                     /locus_tag="VN627_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HXD03284.1"
                     /translation="MSDTELRLAAWRDGSTQAERLSAAVLRLSGYEAIDPQAPIGGPD
                     GGKDILCTKGGVTWTSAVYFPPTPVAFGTIKKKFVSDLAKIPAGSPGFVFVTNRSLTV
                     TQRTTLEALGRSAGKDVDIFHLERLRAVLDSPRGYGVRLQFLGLAMTIEDQLAWFSES
                     DDRVLSAVHVNTRELLAIKTMIQGMAKTGAEIVRTMSVLGLMPSPTPDLLSTANFASA
                     VPAAPITALIDIPLILFVHRLACFDLPIRTVGVLRITELWLANGKGERAQHLQPPPAH
                     KVAPLLQKLCEEWRHDYAAISSGTSPAKLEAITLFHARLLAIHPFADGNGRLARALMM
                     "
BASE COUNT          604 a         1077 c         1127 g          640 t
ORIGIN      
        1 gagcttgccc gtggcagtag ccagcaattc gaaggccatg gcgctggcat gccaggtgcg
       61 gaagtgcttg gcggtgaagg cctcgcccat ggtctcgcgc aggtagtcgt tgacgtcgcc
      121 ggagctgacc ggacacggcg cgcccgcctc gtcgacatac tggaacaggt gctggccagg
      181 caggtcctgc atcgcccgca caaagcgcag cagcgagcgg tcggtgatgg tcatctcgcg
      241 cagtttccca ctcttggcgg taaaccgcag cttgagcgcg cgggggcgga cctcgacgtg
      301 ccgcatccgc agcgtcgtcg ccccgaagct gcggttggcc ctggcatagg cctcgttgcc
      361 cacgcggata ccgccggtgt cgagcaggcg cacgacactg gcgatggcgc gggtgcggca
      421 cagggcccgg acagagagat cctgctccac ccgcttgcgc acccgcggca ggagactgcc
      481 gaagcgggcg gtgtgatcga acttctcgct ctcgcgcgtg aggcggaaca gggggtggta
      541 gcgatactgc ttgcgcccgc gggcatcgat gccggtggcc agcaggtgcc catctccatg
      601 ggggcagaac caggcctgtt cataggccgg cggcagggcg atgcggttga gccggtcgat
      661 ctcggcgcgg tcggtgatgc gctgcccggc tgggtcgtaa taggcccagg caacccggcc
      721 gccccggccc agccgcttgc gggtgatgcc cggctggcga tcgtcgcaga aatgcaggcg
      781 aattccggtg gcggcggcga tagagcgcgg gtccatggcg agcgaacggc tgcggatggg
      841 ttgcagttcc gtgcgggccg cgccacctat gcgggccaga ttggaaagaa aggccgccga
      901 tgtccgatga ctataccccg cccgtggtgt gggaggcccc tgccccggcc gacggcatgg
      961 gtgcgctgtt ctcattcaac cgagcggttt ccggaccgct ccgcgacgag gaactgccgg
     1021 tgggccgcca tccgctgcag ctctattcgc tggcaacgcc caacgggcaa aaagtgggca
     1081 tcatgctgga agagctgctc gcggctggcc acgccggtgc ggaatacgat gcctggctga
     1141 tcaacatctc gcagggcgat cagttcacca gcgggttcgt ggacatcaac cccaacagca
     1201 agatccccgc gctggtcgat cgtagcgggc ctgagccgat ccgggtgttc gagagcgggg
     1261 cgatcctgtt gcatcttgcc gagaaattcg gcgcctttct cccgcgcgag ggcgcggcgc
     1321 gggcggaaac gctgtcatgg ctgatgtggc agatgggcac ggccccgttt attggcggcg
     1381 gcttcggaca cttctatgcc tatgccccga ccaagatcga atatgcgatc gaccgctaca
     1441 cgatggagac caagcggctc ctccatgtgg ccgacacgcg gctggcgcaa tgccgctacc
     1501 tggcagggga cgaatatacc atcgcggaca tggccgtgct gccgtggttc gccgggctgt
     1561 ggcgcggcac ggtgtacaac gaggcgcgga ccttcctctc catggacgac tatgcgcacc
     1621 tcggccggtg ggtggaggaa ctgctcgccc gcccggccgt ggcccgtggc cgcaaggtca
     1681 ccagccttga gatgcccgag cggcattccg ctgcggattt cgacgcgata gtgctctagg
     1741 gcgcttccca agccggttgg gcgcggctat gcgcgccgga ctggtggggc gtcgccaagt
     1801 ggtaaggcac cggtttttgg taccggcatt cgcaggttcg aatcctgccg ccccagccag
     1861 caggtcatcg atttagggct cggttcaggc caatggcacg tcaagcccgc aaatctgcga
     1921 gcttgcgatg ccgaagtgca ctgcggagaa cggtttcgcc gaccaattga agccctaaat
     1981 tgcgggcatt ctcctgccac aaaatggcca gtacagtttg caattcggcc ggtgcgttgg
     2041 actttcctgc cgatcggcgc tcctagtgga aacgtccggc tacgccataa ccagccattc
     2101 gtctggccgc gagccctttc acctacgtca gggcttggga cgatccggtt aggcagcttc
     2161 acaccctgag aaccggttag cggacattcg gatgctcgag tgtcgccgtc gggttccgac
     2221 caatgccagc cgttcgcttc atgtcgggtg aacgtcaggt cctgacgaaa gctgtcattg
     2281 gcgtcagtcg cagcggacga acagcaacgc gtcataaagg gtggctaagc gtggtttgac
     2341 atgacctcga aacctgtcct tggttcaaat cgctctatcg aagcaggtcg gtggaattgg
     2401 cagcgatgaa tgggtaggat agtgcaacaa acgggagggg gggcgatgtc ggatacggaa
     2461 ctgcggctcg ctgcatggag ggatggatca acgcaggccg aacggttgtc tgctgccgtc
     2521 cttcgcctat ccggttacga agccattgat ccccaggcgc cgatcggtgg cccagatggc
     2581 ggcaaggaca tcctctgtac gaagggtggc gtaacttgga cgagtgccgt ctatttccca
     2641 ccgactccgg tcgcgttcgg cactatcaag aaaaaatttg tctcggatct cgccaagata
     2701 ccggctggga gccccggttt cgtattcgtg acaaaccgct cgctcactgt cacgcagcgc
     2761 acgactcttg aggcgctcgg tagaagcgcg ggcaaggacg tggatatctt ccacttggag
     2821 cggctccggg cggttctcga ttcaccccgc ggctatgggg tcaggctcca gttcctgggg
     2881 ctcgccatga cgatcgagga tcaactcgct tggttttcgg aatccgacga tcgcgttctc
     2941 agcgcagtgc acgtcaacac gcgcgagttg cttgcaatca agacaatgat ccagggcatg
     3001 gccaagacgg gcgccgagat cgtgcggacc atgtccgtgc tgggcttaat gccgtcgccg
     3061 accccggacc tgctctccac cgctaatttt gccagtgccg ttcccgccgc accgatcacc
     3121 gcacttatcg acattccgct tatcctcttc gttcatcgtc ttgcctgttt cgatctgccg
     3181 atccgcaccg ttggtgtgct caggatcacc gagctctggc tcgccaacgg caagggcgaa
     3241 cgcgcgcagc acctccaacc cccgcctgcc cacaaggtcg caccgctcct ccagaagctg
     3301 tgcgaggagt ggcgccacga ttacgccgct atcagtagcg gcacctcgcc tgcgaagctg
     3361 gaggcgatca ccctcttcca cgcgcggcta ctcgcgattc atcccttcgc cgacgggaac
     3421 ggccgactgg cgcgggcctt gatgatgc
//
LOCUS       DATNBV010000346         3243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1185114,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000346 DATNBV010000000
VERSION     DATNBV010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185114"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VN627_08260"
     CDS             complement(<1..538)
                     /locus_tag="VN627_08260"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXD03285.1"
                     /translation="MMPARSSRYAIQKRVLVTGGAGFIGSHLCAWLIEAGHSVLCVDN
                     FFTGTRANIAPLLDNPNFELLRHDITFPLYVEVDEIYNLACPASPVHYQFDPVQTTKT
                     SVLGAVNMLGLAKRLRIPILQASTSEVYGDPSVHPQPEDYWGNVNPIGPRSCYDEGKR
                     CAETLFFDYHRQHNLAIKV"
     gene            complement(612..1469)
                     /locus_tag="VN627_08265"
     CDS             complement(612..1469)
                     /locus_tag="VN627_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXD03286.1"
                     /translation="MDLDLGRLLHDQQGSLVLEGFPPPRLLPKPAAPRRLRADGDELR
                     QIREHVLARIAPDQDNPGPAVLSSLTAPALPAEWPKAEFTSPAAAIPRSRAKRVLDVV
                     GAAGLLVLLLPLLFFVGIFVALDGHGPVLFLQRRCGMGGSVFRIFKFRTMITCDDGDT
                     VAQTARADRRITRFGAFLRRTSIDELPQLFNVLTGEMSLVGPRPHAAAHDRTFAQTLP
                     HYAGRYLVRPGITGLAQVRGQRGAIHDPEQLAGRVASDLEYIEKWSIFTDLRILAASV
                     GVVFRPTGG"
     gene            complement(1641..2861)
                     /locus_tag="VN627_08270"
     CDS             complement(1641..2861)
                     /locus_tag="VN627_08270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXD03287.1"
                     /translation="MIAYLVNQYPKVSHSFIRREIAALEQLGTPVFRLALRGWDADLA
                     DPADVAERARTTYLLRGAKPRLLAAALATLAARPLPFLRALRQAVRFARGSDRSLPYH
                     LAYLLEAALLVRALQGRGIAHLHAHFGTNPAEIAALARTLGGPSFSFTVHGPEEFDRP
                     EALKLREKAEGATAVVAISHYCRGQLSRFVAPADVGKIAIVHCGLDEAFLSASPVAPP
                     GNGTRLVCVGRLCAEKSQILLVRALAALRERGIRVDLVLAGDGDLRGEIEALIARECL
                     GDQVTITGWIGSDEVRRLLEQADALVLPSLAEGLPVVIMEAMALGRPVITTWVAAIPE
                     LVRPGVDGLLVAPGDEAGLVEAIGRFIALSPGDRRAMGENARARVRHDHSARVEAGKL
                     RQVFATGNSPGNRM"
     gene            complement(2858..>3243)
                     /locus_tag="VN627_08275"
     CDS             complement(2858..>3243)
                     /locus_tag="VN627_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03288.1"
                     /translation="RAGFATTRLVQLHGLSAEPGWRRYLFSTVLWTALMASIPAGLVL
                     AALAPTRATAAAAVLPALAVCIQWLRLARRIPAARRHPLRALADGGLVLVTKVASFAG
                     MAEFAIRQASRRRDRLIEYKGTPAS"
BASE COUNT          556 a         1028 c         1203 g          456 t
ORIGIN      
        1 cgaccttgat cgcgagattg tgctggcggt gatagtcgaa gaacagggtc tcggcgcagc
       61 gcttgccttc gtcgtagcaa ctgcgcgggc caatcggatt gacgttgccc cagtagtcct
      121 ccggctgggg gtggaccgag ggatcgccgt agacctcgct ggtcgaggcc tggaggatcg
      181 gaatgcgcag ccgcttggcc aggcccagca tgttgaccgc gccgaggacg ctggtcttgg
      241 tcgtctggac cggatcgaac tggtaatgca cgggcgatgc cggacaggcg agattgtaga
      301 tctcgtcgac ctcgacatag agcgggaagg tgatgtcgtg gcgcagcagc tcgaaattgg
      361 ggttgtcgag cagcggggcg atgtttgccc gggtgccggt gaagaaattg tcgacgcaca
      421 gaaccgaatg ccccgcctcg atcagccagg cgcacaggtg ggagccgatg aaccccgctc
      481 cccccgtgac caggacgcgc ttctggatgg catagcggct ggaacgggct ggcatcatgg
      541 aactgtcaac tccgggcgta agggggcgtt cccctggaca tgctgggatg ggagcggcaa
      601 ccggctggcg atcatccgcc ggtcgggcgg aacaccacgc cgaccgaggc ggcgagaatg
      661 cgcaggtcgg tgaaaatcga ccatttttcg atatattcga gatcggacgc gacccggccg
      721 gcgagctgtt cgggatcatg gatcgcgccg cgctgcccgc gcacctgggc caggccggtg
      781 atgccggggc ggaccaggta gcgcccggca tagtgcggca gggtctgggc gaaggtccgg
      841 tcgtgcgcgg cggcatgggg gcgcggcccg accagcgaca tctcgccggt gaggacattg
      901 aacagctgcg gcagctcgtc gatgctggtg cggcgcagga aggcgccgaa gcgggtgatc
      961 cggcgatcgg ccctggcggt ctgcgcaacc gtatcgccgt cgtcgcaggt gatcatcgtg
     1021 cggaacttga atatccggaa caccgagccg cccatgccgc agcggcgctg gagaaacagc
     1081 accgggccat ggccatcgag cgccacgaag atgccgacga aaaacagcag cggcagcagc
     1141 aggacgagca ggcccgccgc tccgaccacg tccagcacgc gcttggcacg gctgcgcgga
     1201 atggccgcgg cggggctggt gaattccgcc ttgggccatt cggccggcag ggctggcgcc
     1261 gtcagggacg agaggacagc cggcccggga ttgtcctgat cgggcgcaat ccgggccagg
     1321 acgtgttcgc ggatctggcg cagctcgtcg ccgtcggcac gcaatcggcg gggagccgcg
     1381 ggcttcggca gcaggcgtgg cggagggaaa ccctccagca cgagcgaacc ctgctgatcg
     1441 tggagcaaac gaccgaggtc tagatccatc tgcgccgatc cttctgcggg cgggagcggc
     1501 gcagatcggg caatattggc gtcgatacga atgtctgcca cggctaccct catctgagcc
     1561 cttcggccgc gattgcttgc gccgtcggag gaaagcctgc ccggggggat agttcccggc
     1621 ggttcgccgc agaaaattga ttacattcgg ttcccgggcg aattcccggt cgcgaacacc
     1681 tggcgcagtt tgccagcctc aacacgcgcg gaatggtcgt ggcggaccct ggcgcgggcg
     1741 ttttcgccca tggcgcggcg gtcgccgggg gagagggcga tgaaccggcc gatcgcctcg
     1801 acgagcccgg cctcgtcgcc cggggcgacc agcaggccgt ccactccggg gcgcaccagt
     1861 tcgggaatgg ccgcgaccca ggtcgtgatg accggacggc caagcgccat ggcctccatg
     1921 atcaccacgg gcagcccctc ggccaggctg ggcaggacca gggcatcggc ctgctcgagc
     1981 agccggcgaa cttcgtcgct gccgatccaa ccggtgatcg tgacctggtc gccaaggcac
     2041 tcccgggcaa tcagcgcttc gatctcgccg cgcaggtcgc catcgccggc gaggacgaga
     2101 tcgaccctga tgccgcgctc tcgcagggca gctaaggcgc ggaccagcag gatctgcgac
     2161 ttttccgcgc agagccggcc aacgcagacc agccgcgtgc cgttgcccgg cggagccacc
     2221 ggactggcgg acaggaatgc ttcatcgagc ccgcagtgga cgatggcgat cttgccgaca
     2281 tcggcgggcg ccacgaaccg gctgagctgg ccgcgacaat aatgggagat ggcaaccacc
     2341 gccgtcgcac cctccgcctt ttcccgcagt ttcagagctt cgggccggtc gaattcctcc
     2401 gggccgtgga cagtgaagct gaacgagggg ccgccgaggg tgcgggccag ggccgcgatt
     2461 tcggccgggt tggtgccgaa atgggcatgg aggtgggcaa tgccgcggcc ttggagcgcg
     2521 cggaccagca gggcggcttc gagcaggtag gccaggtgat agggcagcga cctgtcggag
     2581 ccccgggcga accggaccgc ctgccgcagg gcgcgcagga aggggagcgg gcgagcggcg
     2641 agggtggcga gcgcggcggc gagcagccgg ggcttcgcgc cgcgcagcag gtaggtcgtc
     2701 cgcgcgcgct cggcgacgtc cgcgggatca gccaggtccg catcccagcc gcgcagggcc
     2761 aggcggaaga ccggcgttcc cagctgttcc aaagcggcaa tttcgcggcg gatgaaactg
     2821 tggctgacct tggggtactg gttgaccaga taggcgatca cgacgccggc gtgcccttgt
     2881 attcgatcag ccggtcgcgg cggcgggagg cctgccggat tgcgaactct gccatgcccg
     2941 cgaaggaggc gaccttcgtc accaggacaa ggccgccatc ggccagggcg cgcagcggat
     3001 ggcgccgggc cgcgggaatc cgccgggcga ggcgcagcca ctggatgcaa accgccagcg
     3061 ccgggagcac cgccgccgcg gcagtggcgc gggtgggggc caaggctgcc aacaccagcc
     3121 cggccgggat cgaggccatc agcgcggtcc acaggaccgt gctgaagagg tagcgccgcc
     3181 agcccggttc ggcggaaaga ccgtggagct gaacgagccg cgtggtcgcg aacccggccc
     3241 ggc
//
LOCUS       DATNBV010000347         4971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3404657,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000347 DATNBV010000000
VERSION     DATNBV010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3404657"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..445
                     /locus_tag="VN627_08280"
     CDS             2..445
                     /locus_tag="VN627_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HXD03289.1"
                     /translation="MILGAAAALLTGASAPPQDVSVMVSGLRSAQGKVLACITANPAM
                     FPDCGKDPAGRHIAVAVPAGGAVAFDFGPMPVGRYAISLFHDENANGKLDKMMMLPRE
                     GFGFSRDAPVRFGPPSFAAAAFSVDGAPVRQVIRLRYMLGGSPRR"
     gene            468..1778
                     /locus_tag="VN627_08285"
     CDS             468..1778
                     /locus_tag="VN627_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD03290.1"
                     /translation="MTDRAMLAIGWSMADGSDDPARRDQPPTAQRPGTFAPFGFAAFR
                     NVWAANTLSQIGAQIQLVGAGWLMTELTSEHRMVAGVQAANAMAMLFLSVPAGALADN
                     FDRRRIMLLANWFMLLVSAVLAVQAWRGQLTPWSLLGFSLLVGAGMTMQLPAWQASVR
                     ALVDGRVLPQAISLNSVAFNLARTVGPALGGMILAVAGVSAAFAVNALSYVALIAVFA
                     RWRPRVLPLTRQPLLPSIWAGLIGYAASPPLRRLLLRGMAYTCGAMAVQALLPVVVRN
                     LHGGETDFGVLLGAFGVGSVAGAIFAPTLRRRAGAEWTIAAATATVSLGLAGLSVASS
                     VLTALPCVLVGGMAWALSMTTMNVSTQLRAPEDMLGRIMSINQATVLGAAAIAAWGWG
                     TLADFAGLRTALGMAAGYMALSGLFLHFLAPMPARGEGVVLGRA"
     gene            1861..3180
                     /locus_tag="VN627_08290"
     CDS             1861..3180
                     /locus_tag="VN627_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03291.1"
                     /translation="MDRLRGDFSDYGDAEGYDPLEDEIGHEAPPPSVGQDERRLQVRA
                     YNHWASLLGDRSFPLIGDLAIGQLPDFDPHAVLLDFAEGVDHPLVRYVGPALAEESGC
                     AGQALRRLEDVPGRTLLSRITDHYLQIFGNQSPVGFEAEFVNQRSRTILYRGILLPFS
                     RDAESTGIDCIYGVINWKELADQQTTDELMHAIDRALTPRAPSRRDAEPLTEWADGPA
                     DLAQEPLDLGAFSLDEPTGWNSTAEASLADILASARDLAHQAAAAGERSRQALYEAIG
                     RAHEFALAADADPETLHELVADAGLVMQERAPLIPLVKLVFGADYDKTRLTEYATVIA
                     HARRIALPGGELARFLAVQPGGLKGVVQIERRMRRAASGEPQAPARDRLAFRLRQLPT
                     RSLDTLSAEGEEFALVLARRTPEGRVVMLGEVADPGLVERAARKLLD"
     gene            3285..4670
                     /locus_tag="VN627_08295"
     CDS             3285..4670
                     /locus_tag="VN627_08295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXD03292.1"
                     /translation="MHPPSSPPSALRLLRLAAAFLALAVFIARPAAAQSILRDAETEA
                     LLHDMANPLVKAAGLDPRNVDVVLVGDNTINAFVMNGQTVYLNAGLINAADSANQVQG
                     VIAHELGHVTGGHAISGEQGAKSAGTISLLSLLIGAAAMAAGGGDAGMAAIMAGQSAA
                     MGKYLAFNRVQEAQADAAGATFLAKSGISGRGSIEFFKKLEAEGYKRGYSQADQDPFM
                     STHPLESDRIALLEETYKKDPAWNRPSDPVLEARFRRAKAKLYGYLAEPEAVLAAYPV
                     SDTSVPGRYARAYAWHKQALMDKAMGEVDALLQTAPADPYFLELKGQILLESGKPAEA
                     LAALRRATELTGNQPLIATTFGHALVATEDPKNLAEAQRVLRAAVARDRENPDAWYQL
                     GVVYNANGDGPRARLASAEQQVLQQQWPLALGSAETALSGLPTGSADAIRAQDIAMQA
                     RAEIERRKKRK"
     gene            4681..>4971
                     /locus_tag="VN627_08300"
     CDS             4681..>4971
                     /locus_tag="VN627_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03293.1"
                     /translation="MANFTRNRAFFVNAAVALAFAAIGALGGWVWESHRIGADPAARL
                     AGEDRAAIEQVIHDYLMAHPEVLPKAMAELERRQNAHQLASVRGDVERAVPGA"
BASE COUNT          715 a         1806 c         1635 g          815 t
ORIGIN      
        1 cctgatcctc ggcgcggccg cggccctgct gaccggcgcc tcggcgccgc cgcaggatgt
       61 cagcgtgatg gtcagcggcc tgcgcagcgc gcagggcaag gtgctcgcct gcatcaccgc
      121 caacccggcg atgttccccg attgcggcaa ggacccggcg gggcggcaca ttgcggtcgc
      181 cgtgcccgcg ggcggcgcgg ttgccttcga tttcgggccg atgccggtgg gccgctacgc
      241 aatctcgctg ttccatgacg agaacgccaa cggcaagctc gacaagatga tgatgctccc
      301 gcgcgagggg tttggcttct cgcgcgatgc gccggtccgc ttcggaccgc ccagcttcgc
      361 cgcggctgcc ttctcggtgg acggcgcccc ggtccgccag gtgattcgcc tgcgctacat
      421 gctgggtggt tcgccccggc gttgaggcct tattcgtcgc ctgccggttg accgatcgcg
      481 caatgctggc catcggctgg tctatggccg atggcagcga cgatccagcc aggcgggacc
      541 agccccccac cgcgcagcgc ccgggcacct ttgcgccatt cgggttcgcc gccttccgca
      601 atgtctgggc ggccaacacg ctgtcgcaga tcggcgccca gatccagctc gtcggcgccg
      661 gctggctgat gaccgagctg accagcgaac accgcatggt cgcgggggta caggcggcca
      721 acgccatggc catgctgttc ctttcggttc cggcgggcgc cctagccgac aacttcgatc
      781 gccgccggat catgctgctg gcgaactggt tcatgctgct ggtctcggcg gtccttgccg
      841 ttcaggcctg gcgagggcag ctcacgccct ggtcgctgct tggcttcagc ctgctggtcg
      901 gcgcgggcat gaccatgcag ttgccggcct ggcaggcttc ggtccgcgct ctcgtcgacg
      961 ggcgggtgct gccgcaggcg atctcgctca attcggtggc gttcaacctg gcgcggaccg
     1021 tgggcccggc cctgggcggg atgatcctcg ccgtggcggg ggtctcagcc gcctttgcgg
     1081 tgaatgcatt gagctacgtc gccctcatcg cggtgttcgc acggtggcgg ccgcgagtcc
     1141 tgccgctcac gcgccagccg ctgctcccct cgatatgggc cgggctgatc gggtatgccg
     1201 cctcgccgcc gctgcggcgg ctgctgttgc gcggcatggc ctatacttgc ggggccatgg
     1261 ccgttcaggc cctgctgccg gtcgtggtgc gcaatctcca tggcggggag accgatttcg
     1321 gcgtgctgct cggcgctttc ggggtcggct cggtcgcggg cgccattttc gccccgacgc
     1381 tgcgccgccg cgcgggagcg gaatggacca ttgccgcggc gacggccacc gtcagcctcg
     1441 gcctcgccgg cctgtccgtc gcttcctcgg tgctcaccgc gctcccctgt gttcttgtcg
     1501 ggggcatggc ctgggccctg agcatgacga ccatgaacgt gagcacccag ctccgcgcgc
     1561 cggaagacat gctcgggcgg atcatgtcga tcaaccaggc caccgtcctc ggcgccgccg
     1621 ccatcgcggc ctggggctgg ggaaccctgg ccgatttcgc aggcctgcgc acggcccttg
     1681 gcatggcggc cggctacatg gcgctgagcg gcctgtttct gcacttcctc gcccccatgc
     1741 ccgcgcgcgg cgagggcgtg gtgctggggc gcgcctgagc ggcaaccggc gcaactttaa
     1801 ccctttttca accatgttgc gccaagaccg gtcccctgat ctatcaggga actcgggtaa
     1861 atggacaggt tgcgcggcga tttctcggac tatggcgatg ccgagggtta cgatccgctc
     1921 gaggacgaga tcggccatga agcgcccccg ccctccgtgg ggcaggacga gcgccgcctc
     1981 caggtgcgcg cctataacca ctgggccagc ctgctgggcg accggtcgtt cccgctgatc
     2041 ggcgaccttg ccatcgggca actgcccgat ttcgatcccc atgccgttct gctcgatttc
     2101 gccgaagggg tcgatcaccc gctcgtgcgc tatgtcggcc cggccctcgc cgaggaatcg
     2161 ggctgtgccg gccaggccct gcgccggctg gaagacgtgc ccggccgcac cctgctgtcg
     2221 cgcatcaccg atcattacct gcagatcttc ggcaaccagt cgccggtcgg cttcgaggcc
     2281 gaattcgtca accagcgcag ccgcaccatt ctttatcgcg gcattctgct gccgttctcc
     2341 cgcgatgccg aaagcaccgg gatcgactgc atctacggcg tgatcaactg gaaggaactg
     2401 gccgaccagc agaccaccga cgaactgatg cacgcaatcg accgggctct caccccgcgc
     2461 gcgccgtcgc gccgcgatgc cgagccgctg accgagtggg ccgatggccc ggccgacctt
     2521 gcccaggagc cgctcgacct cggcgccttc tcgctcgacg agccaaccgg gtggaattcc
     2581 accgcggaag ccagccttgc cgatattctc gccagtgcgc gcgatctggc ccaccaggcc
     2641 gccgccgccg gcgagcgcag tcgccaggcg ctgtacgagg cgatcggccg ggcccatgaa
     2701 tttgccctcg ccgccgacgc cgatccggag accttgcacg aactcgtcgc cgatgccggg
     2761 ctcgtcatgc aggagcgcgc gccgttgatc ccgctggtca agctcgtgtt cggggcagac
     2821 tacgacaaga cccggcttac cgaatatgcc accgtcatcg cccatgcccg ccgcattgcc
     2881 ctgccgggcg gcgaactggc gcggttcctc gccgtccagc cgggcgggct gaaaggcgtg
     2941 gtccagatcg aacgccggat gcgccgcgcc gcctcgggcg aaccgcaggc tcccgcgcgt
     3001 gaccggctgg ccttccgcct gcgtcagttg ccgacccgtt cgctcgacac cttgtctgcc
     3061 gaaggggagg agtttgccct cgtccttgcc cggcgcacac cggaaggccg cgtggtcatg
     3121 cttggcgaag tcgccgatcc ggggttggtg gagcgcgccg cgcgcaagct gctcgactga
     3181 ggcctcgccg atagggcgcg caccccgccc gtaaaccggc tatggcctcg gccatggcca
     3241 gttttcacat gaggttcagg cgcgcgggtg atggtgcggc ggcaatgcac ccgccttctt
     3301 cgcccccctc cgccctgcgc ctgctgcgcc ttgccgcagc cttcctggcc ctggcggtct
     3361 tcatcgcccg cccggccgcc gcccagtcga tcctgcgcga tgcggaaacc gaggccctgc
     3421 tgcacgacat ggccaatccg ctggtcaagg ccgccgggct ggatccgcgc aatgtcgatg
     3481 tcgtcctcgt cggcgacaac acgatcaacg ccttcgtcat gaacgggcag acggtctacc
     3541 tcaatgccgg cctgatcaac gcggcggata gcgccaacca ggtccagggc gtcatcgctc
     3601 acgaactcgg ccacgtcacc ggcggccatg ccatcagcgg cgaacagggg gccaagtccg
     3661 ccggtacgat cagcctgctc tcgctgctca tcggcgctgc ggccatggcc gctggcgggg
     3721 gcgatgccgg catggcggcc atcatggccg ggcaaagcgc ggccatgggc aaatacctcg
     3781 ccttcaaccg ggtccaggaa gcccaggccg atgccgccgg ggccactttc ctggccaagt
     3841 ccggcatcag cgggcgcggc tcgatcgagt ttttcaagaa gctcgaggcc gaaggctaca
     3901 agcgcggcta cagccaggct gaccaggacc ccttcatgtc cactcacccg ctggagagcg
     3961 accgcatcgc cctgctggag gaaacctaca agaaggaccc ggcctggaac cgccccagcg
     4021 atcccgtgct ggaagcccgc ttccgccggg ccaaggccaa gctctacggc tacctggccg
     4081 aacccgaagc cgtgctcgcc gcctatccgg tcagcgatac ctcggtgccc ggacgctacg
     4141 cccgcgccta tgcctggcac aagcaggcgc tgatggacaa ggcaatgggc gaggtcgacg
     4201 ccctgctgca aaccgcgccg gccgatccct attttctcga gctcaagggc cagatcctgc
     4261 tcgaatccgg caagcccgcc gaagccctgg ccgccttgcg ccgggcgacc gagctgaccg
     4321 gcaaccagcc gctgatcgcc accaccttcg gccacgccct cgtcgctacc gaggatccga
     4381 agaaccttgc cgaagcgcag cgcgtgctca gggccgcggt cgcgcgtgat cgcgagaatc
     4441 ccgatgcctg gtaccagctt ggcgtggtct acaacgccaa tggcgatggc ccgcgcgccc
     4501 ggctggccag cgccgagcag caggtcctgc agcagcagtg gccgcttgcc ctcggcagcg
     4561 ccgaaaccgc gctttccggg ctgccgacgg gctctgccga tgcgatccgc gcacaggata
     4621 tcgcgatgca ggcccgcgcc gagattgaac ggcgcaagaa gcgcaagtaa gccagctgtc
     4681 atggccaatt tcacccgcaa ccgcgcgttc ttcgtcaatg ccgccgtcgc cctcgccttc
     4741 gccgcgatcg gcgcccttgg cgggtgggtg tgggaatccc accgcatcgg tgccgatccc
     4801 gccgcgcgcc tcgccgggga agatcgcgcc gcgatcgagc aggtgatcca cgattacctg
     4861 atggcccacc ccgaagtcct gcccaaggcc atggccgagc tggagcgccg ccagaacgcg
     4921 caccagctcg ccagcgtgcg cggcgatgtg gagcgggccg tgcccggcgc g
//
LOCUS       DATNBV010000348         7544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2119919,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000348 DATNBV010000000
VERSION     DATNBV010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119919"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VN627_08305"
     CDS             complement(<1..359)
                     /locus_tag="VN627_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXD03294.1"
                     /translation="MAKPVASSLVIERTYTAGVDELWALWTTKKGFESWWGPEEFRVE
                     VHLIEPCPGGALHYDMVADTPAAVAAMEGMGAPIRQPCRGTFSEYLLHERLTLTQVID
                     FLPGVAPYDSTIQVDVLA"
     gene            complement(352..696)
                     /locus_tag="VN627_08310"
     CDS             complement(352..696)
                     /locus_tag="VN627_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXD03295.1"
                     /translation="MHIDLFLTLADPTRRCVIDALRGGERQVNELVTAVGIHQSGVSR
                     HLRILSEAGFVTMRPEGSRRLYSLRPEPFREIEAWLTQYRQIWDARLDRFAAALDRQQ
                     KNGTEQRSLSDG"
     gene            complement(849..2258)
                     /gene="glnA"
                     /locus_tag="VN627_08315"
     CDS             complement(849..2258)
                     /gene="glnA"
                     /locus_tag="VN627_08315"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HXD03296.1"
                     /translation="MSKASDVLKRIKDEEIQWVDLRFTDPKGKWQHLTMVAGVLTEGE
                     LEDGLMFDGSSIEGWKAINESDMILKPDLDAIYVDPFSATPMLIVNCDIVEPSTGELY
                     GRDPRSCAKRAEAYVRAGGFGDTVYVGPEAEFFVFDDVRFYDGYDGNGFKIDDIELPT
                     NSNREYEAGNLAHRPRAKGGYFPVAPVDSCVDIRGEMVATMLEMGLPCDKHHHEVASA
                     QHELGLTFGTLVQTADRMQVYKYVVHQVAQAYGKTATFMPKPIMKDNGSGMHTHISIW
                     DKGNPLFAGNGYAGLSDMCLYFIGGVIKHAKALNAFTNPTTNSYKRLVPGYEAPVLLA
                     YSARNRSASCRIPYGAGAKAKRVEFRFPDAMANPYLCYSALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPAELAQVPTVCGSLREALESLEADHAFLLEGGVFTKDQIESYVELKWPE
                     VLRWETTPSAVEFDMYYSA"
     gene            complement(2365..2703)
                     /locus_tag="VN627_08320"
     CDS             complement(2365..2703)
                     /locus_tag="VN627_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXD03297.1"
                     /translation="MKKIEAIIKPFKLDEVKEALHEIGVSGITVTEAKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLEVVVSDALAERVVEAIAQAAQTGRIGDGKIFVIPIESALRI
                     RTGERDDAAI"
     gene            3060..3818
                     /locus_tag="VN627_08325"
     CDS             3060..3818
                     /locus_tag="VN627_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03298.1"
                     /translation="MSGIADMMGLAGKVALVTGGASGIGEGIAEVLARAGARVVVLDR
                     NLPQAEAVAAALGGGAFARALDVSDEAAVCATLAAVVAEAGAPWLLVNCAGVQDRVAL
                     LDGTGADWDRNYEVNLRGAFFCLREAAKAMIAAGSGGRIVNITSISATAPVMVGLAAY
                     TASKHGLLGLTRSAAMELMEHAITVNSVQPGGVKTPGAANATGPVGGRALDTPVMGHN
                     TPHEIGAAVLYFASALAGRTTGQTLAVDSGFLLG"
     gene            complement(3826..4755)
                     /gene="argC"
                     /locus_tag="VN627_08330"
     CDS             complement(3826..4755)
                     /gene="argC"
                     /locus_tag="VN627_08330"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HXD03299.1"
                     /translation="MTHSVFIDGAAGTTGLEIADRLAGRPEFEMIVLDDARRKLPEAR
                     REALHAADFAILCLHDDAAREAVAMAEGAKVRIIDASTAHRVSDGWTYGLPEVVGRDA
                     VAGASRIANPGCYPTGFIALMAPLVRAGLLPASFPYTANAVSGYSGGGKALIERFEAD
                     TDIAFRDYGLAMGHKHVPEMQRYAGLVHPPVFAPAVIAAHRGMVVEVPLPLAAIPGAA
                     GPDALREALAAFYAETPLVRVRTDAPDELLLRKSTPAWDGLELYVFGARDGSQARLVA
                     VLDNLGKGASGAAVQSLNLMAGLPETAGLSLPA"
     gene            complement(4765..5157)
                     /locus_tag="VN627_08335"
     CDS             complement(4765..5157)
                     /locus_tag="VN627_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXD03300.1"
                     /translation="MGSGQELPHDGSSELTAITTPGASFALAGPSAKIDAAHNPVRGD
                     LAHIRLAGKVFVPHYVVPLPHVVNGAGAPLHTAGKTEADLVAQLPTGTVFNVLDISGT
                     GDAAWAWGQVGEDGVVGYVPLAALHPQA"
     gene            complement(5217..6614)
                     /locus_tag="VN627_08340"
     CDS             complement(5217..6614)
                     /locus_tag="VN627_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HXD03301.1"
                     /translation="MTDRNALIQPDRGQQAIPIHLVNKDSFPDWLKRLSAGQRAALAA
                     QKFEGGGYEHAIVPDGDGWFVAAGVADPDALSSWCLAKLADVLPGGTYRLADHATGRA
                     LFGWVSGQYRFERYRSEAKDEDPRVLLTKDARAIDPALREAAAVELVRDLVNTPAEDM
                     GPAELEAVAAALAKAHPGRNGAELHITKGDALEQDFPMVHMVGRAAGRAHAPRMIELE
                     WGDPAHSRVAIVGKGICFDSGGLDIKPSSAMLLMKKDMGGAAHALALARLIMEQGLPV
                     RLHCLVAAAENAISGNAFRPGDVLRSRAGLTVEIGNTDAEGRLVLGDALARAGEANPE
                     LVIDFATLTGAARVALGPDLPALFARQDATAEALLAAGSAHDDPCWRLPLWDGYKEHL
                     KSDVADLNNGGGNGMAGATTAALFLDKFVPEGIDWAHFDTYAWRPAAKPGRPKGGDAL
                     GLRAAWHMLQARYGG"
     gene            complement(6686..6994)
                     /locus_tag="VN627_08345"
     CDS             complement(6686..6994)
                     /locus_tag="VN627_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXD03302.1"
                     /translation="MKPTDLTELKANAARMAGRIKLMSHPERLLMLCRMDEGEVSVNE
                     LVALTGLSQSSVSQHLAMLREEGVVSIRGEAQTRWYSLSDPLVRGLIHAMCELCAQHC
                     "
     gene            complement(7048..7383)
                     /locus_tag="VN627_08350"
     CDS             complement(7048..7383)
                     /locus_tag="VN627_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXD03303.1"
                     /translation="MFGFGKKVAHREITPSELELLIAEDRALVVDVREPGEFAQGHIP
                     GAVNMPLSGFQPSRLPDPAGKTIVLNCQGGKRSGMALEKCGAARAEIDTHLAGGFGAW
                     AQSGFPVER"
BASE COUNT         1203 a         2395 c         2644 g         1302 t
ORIGIN      
        1 gccaagacat ccacttggat agtgctgtcg taaggtgcga cgccgggcag aaagtcgatc
       61 acctgggtca atgtcagccg ttcgtgcaga agatattcgg agaatgtgcc gcggcagggc
      121 tgcctgatcg gcgcgcccat cccttccatt gcggccaccg ccgctggcgt gtctgccacc
      181 atgtcataat gcagtgcgcc gcccgggcat ggctcgatca gatggacctc aacgcgaaat
      241 tcctcggggc cccaccagga ttcgaagcct ttcttcgtcg tccacagtgc ccagagttcg
      301 tccaccccgg ctgtgtatgt gcgttcgatt acgaggctgg acgctaccgg cttagccatc
      361 actcaagctc ctctgttcgg tgccattctt ctgttggcgg tccagcgctg cggcgaaccg
      421 gtcaaggcgg gcatcccaga tttgccgata ctgcgtgagc catgcctcga tttcgcggaa
      481 gggctccggt cgtagcgaat atagtcgccg cgatccctcc ggtcgcatgg tgacgaaccc
      541 ggcttcggac aggatgcgca gatgtctcga tacgccggac tgatggatac cgaccgcggt
      601 gaccaattcg ttcacttggc gctccccgcc gcgcagtgcg tcgatcacgc atcttcgggt
      661 gggatcggca agggtgagga acagatcaat atgcacatct atgcatatgt atgatgatgc
      721 atgtctgatc aatccctttt cctatcgcgt ttaccggatg tcggctttcg accggctcgg
      781 cgcgtgcccc aacgaaaaag gcgccccggt ttcccggagc gcccttttgc atggtctgtc
      841 ccgcaggatc aggccgagta gtacatgtcg aactcgacgg ccgacggcgt ggtttcccag
      901 cgcagcactt ccggccactt cagttcgacg taggactcga tctggtcctt ggtgaagacg
      961 ccgccttcga ggaggaaggc gtgatcggct tcgagcgatt ccagggcttc gcggagcgaa
     1021 ccgcagacgg tcggcacctg ggccagttcg gccggcggca gatcgtagag gttcttgtcc
     1081 atggcttcgc ccggatggat cttgttcttg atcccgtcga ggccggccat gagcagggcc
     1141 gagtagcaca ggtaggggtt ggccatcgcg tcggggaagc ggaattccac gcgcttggcc
     1201 ttggcgccgg cgccgtaggg gatacggcag gaagccgaac ggttgcgggc cgagtaggcc
     1261 agcagcacgg gggcttcata gcccggcacc aggcgcttgt agctgttggt ggtcgggttg
     1321 gtgaaggcgt tcagggcctt ggcgtgcttg atgacgccgc cgatgaagta caggcacatg
     1381 tcggacagcc cggcatagcc gttgccggcg aacagcgggt tgcccttgtc ccagatcgag
     1441 atgtgggtgt gcatgcccga gccgttgtcc ttcatgatcg gcttcggcat gaacgtggcc
     1501 gtcttgccat aggcctgggc gacctggtgg acgacgtact tgtagacctg catgcggtcg
     1561 gccgtctgca ccagggtgcc gaaggtcagg ccgagctcgt gctgggcgct ggcgacttcg
     1621 tggtggtgct tgtcgcaggg caggcccatt tcgagcatgg tggcgaccat ctcgccgcgg
     1681 atatccacgc agctgtcgac cggggcgacc gggaagtagc cgcccttggc gcgcggacgg
     1741 tgagccaggt tgccggcttc gtattcgcgg ttcgagttgg tcggcagctc gatgtcgtcg
     1801 atcttgaagc cgttgccgtc gtagccgtca tagaagcgca cgtcgtcgaa gacgaagaat
     1861 tcggcttccg ggccgacgta gacggtgtcg ccgaagccgc cggcgcggac ataggcttcc
     1921 gcgcgcttgg cgcaggagcg cggatcgcgg ccgtagagtt cgccggtcga cggctcgacg
     1981 atgtcgcagt tgacgatcag catcggcgtg gccgagaacg gatcgacgta gatggcatcg
     2041 aggtcgggct tgaggatcat gtccgactcg ttgatggcct tccagccttc gatcgacgaa
     2101 ccgtcgaaca tcaggccgtc ttccagctca ccctcggtga ggacgccggc gaccatggtg
     2161 aggtgctgcc acttgccctt cgggtcggtg aaacgcaggt cgacccactg gatttcctcg
     2221 tccttgatgc gcttcaggac gtcgcttgcc ttgctcattc gtgatccctc tttctcggat
     2281 tttttccgcc gttcggggct gcccgaagtg gcgcgaaact gtgatggaag ttcaacccct
     2341 cgaccggtga tcgaccttca aaggtcagat cgccgcgtcg tcccgctcgc ccgtacggat
     2401 gcgcagcgcg ctttcgatcg ggataacgaa gatcttgccg tcgccgatgc gcccggtctg
     2461 cgcggcctgg gcgatcgcct cgaccacgcg ctcggccagg gcgtcgctca cgacgacctc
     2521 gagcttcacc ttgggcagga agtcgacgac atattcggca ccgcgataaa gctcggtgtg
     2581 gcccttctgc cggccgaagc ccttggcctc ggtgacggtg atgccggaca cgccgatttc
     2641 gtgaagcgcc tccttcacct cgtcgagctt gaacggcttg atgatagcct cgatcttttt
     2701 cacgtttgcc agacccctgc gcggcgtccc ggtgagagcg cccgggcatt gctgcctggg
     2761 gcccgccgga gcggatgtcc aattgccttg gccattccga tatcaagaat cgtgccaagg
     2821 cgcgtagtgc tgactaggcc aagctagccg ccgcgaaaag cccggaattg accgattcgc
     2881 agcacgcccc ccaagtcctg cccaggctga tctggctgac agtcaagctt cctgccgcat
     2941 atttgtgcag ccctgcctga aaattgagca agtgcgcggg taatgtcgcc gctgctggac
     3001 agttcgggac ggcgcggcca agctatccac gactggataa cgaacgatgg agagcgggcg
     3061 tgagcggaat tgcagacatg atgggtctgg cagggaaagt ggcgctggtc accggcggcg
     3121 cttcaggcat cggcgagggc attgccgagg tgctggcacg ggccggagcc cgcgtggtgg
     3181 tgcttgaccg caacctgccc caggccgagg ccgtggccgc ggcgcttggc ggcggcgcct
     3241 ttgcccgcgc actcgacgtg tccgacgagg cggcggtctg cgccacgctg gccgcggtgg
     3301 tcgccgaagc gggggcgccg tggctgctgg tcaactgtgc cggggtgcag gaccgggtcg
     3361 ccctgctcga cggaaccggc gcggattggg accgcaatta cgaggtcaac ctgcgcggcg
     3421 cgttcttctg cctgcgcgag gcggccaagg cgatgatcgc ggccggatcg ggcgggcgga
     3481 tcgtcaacat cacctcgatc tccgccaccg caccggtcat ggtcgggctg gcggcctata
     3541 ccgcctcgaa gcacggcctg ctcggcctga cccgcagcgc cgcgatggag ctgatggagc
     3601 acgcgatcac cgtgaactcg gtccagccgg gcggggtcaa gacccccggt gcggcgaatg
     3661 ccacggggcc ggtcgggggc cgcgcgctcg ataccccggt gatgggccac aacacgccgc
     3721 acgagatcgg cgcggcggtg ctctatttcg cttcggcgct ggccgggcgg acgaccgggc
     3781 agaccctcgc cgtcgattcc ggcttcctgc tcggctgagc gcggatcagg cggggagcga
     3841 gagcccggcg gtttcgggca ggccggccat caggttcagg ctctgcaccg ccgcgccgct
     3901 ggcgcccttg ccgagattgt cgagcacagc gacgagccgg gcctgcgagc cgtccctggc
     3961 gccgaacacg taaagctcca gcccatccca cgcgggggtg gacttgcgca gcagcagttc
     4021 gtcgggcgcg tcggtgcgga cgcggaccag cggggtttcg gcatagaagg cggcgagagc
     4081 ctcgcgcagg gcgtcgggac cggcggcgcc ggggatggcg gccagcggca gcgggacttc
     4141 gacgaccatg ccgcggtggg cggcgatgac ggcgggggcg aagaccggcg ggtgaacgag
     4201 accggcgtag cgctgcatct cgggcacgtg cttgtggccc atggcgaggc catagtcgcg
     4261 gaaggcgatg tcggtatcag cctcgaaccg ctcgatcagg gccttgccgc cgccggaata
     4321 acccgagacg gcgttggccg tgtaggggaa gctggccggc agcaggccgg cgcggaccag
     4381 cggggccatg agcgcgatga acccggtcgg atagcagccg ggattggcga tccggctggc
     4441 accggcgacg gcatcgcgcc ccacgacttc gggcaggcca taggtccacc cgtccgacac
     4501 gcggtgcgcg gtcgaggcat cgatgatccg caccttggcg ccctcggcca tggcgacggc
     4561 ctcgcgcgcg gcatcgtcgt gcaggcacag gatcgcgaaa tcggcagcat gaagggcttc
     4621 gcggcgggct tcgggcagct tgcgccgggc atcgtcgagc acgatcattt cgaactcggg
     4681 ccggccggcg agccggtcgg caatctcgag accggtcgtg ccggcggcgc cgtcgatgaa
     4741 cacgctgtga gtcatgcgcg cagcttacgc ctgggggtgg agcgcggcca gcgggacata
     4801 gcccacgacg ccatcctcac ccacctggcc ccaggcccag gcggcatcgc cggtgccgga
     4861 aatatcgagc acgttgaaca cggtcccggt cggcaactgg gcgacgaggt cggcctcggt
     4921 cttgccggca gtgtggagcg gggcgccagc gccgttcacc acgtggggca gcggcacgac
     4981 atagtgtggc acgaaaacct tgccggcgag ccggatatgc gcaaggtcgc cgcgcacggg
     5041 attgtgcgca gcgtcgatct tcgcgctcgg cccggcgagg gcgaagctgg cccccggcgt
     5101 ggtgattgcg gtcagttccg atgacccgtc gtgcggcaat tcttgtccag accccatcag
     5161 gattgaaggc gcgcgcttag acaatagtgt gcccgagggc aaggtgatgg gggcgcttac
     5221 cccccatacc gcgcctgcag catgtgccaa gccgcgcgca ggcccagcgc atcgccgccc
     5281 ttgggccggc cgggcttggc cgccgggcgc caggcgtagg tatcgaaatg ggcccagtcg
     5341 atgccttcgg ggacgaattt gtcgaggaag agggccgcgg tggttgcccc ggccatgccg
     5401 ttgccgccgc cattgttgag gtcggccacg tcactcttga ggtgttcctt gtagccgtcc
     5461 cacaggggca ggcgccagca ggggtcgtca tgggcgctgc cggcggccag cagggcctcg
     5521 gcggtggcgt cctgccgggc gaacagggcc ggaaggtctg gcccgagcgc gacccgcgcc
     5581 gctccggtga gcgtggcaaa gtcgatcacc agctcggggt ttgcctcgcc ggccctggcc
     5641 agcgcatcgc cgagtaccag ccggccctcg gcatcggtat tgccgatctc cacagtaagc
     5701 cccgcgcggc tgcgcaggac atcgccgggg cggaaggcat tgccggaaat cgcgttctcc
     5761 gccgccgcga cgaggcagtg caggcggacc ggcaggcctt gttccatgat gaggcgggcg
     5821 agggcgaggg cgtgggcggc gccgcccatg tccttcttca tcagcagcat ggccgaagag
     5881 ggcttgatat cgagcccgcc cgaatcgaag cagatcccct tgccgacgat ggcgacgcgg
     5941 gaatgggccg ggtcgcccca ttcgagctcg atcatgcgcg gggcatgagc ccggccggcg
     6001 gcgcggccga ccatgtggac catcgggaaa tcctgctcca gcgcatcgcc cttggtgatg
     6061 tggagttctg ccccgttacg ccccgggtga gccttggcga gggcggcggc gacggcttcc
     6121 agctcggccg ggcccatgtc ctcggcgggc gtgttgacga gatcgcggac cagttcgacg
     6181 gcggcggctt cgcgcagggc gggatcgatc gccctggcgt ccttggtgag gagcacgcgc
     6241 gggtcctcgt ccttggcctc gctgcggtag cgctcaaacc ggtactgccc ggacacccag
     6301 ccgaacaggg cccgcccggt ggcgtgatcg gccaggcggt aggtgccgcc cgggaggacg
     6361 tcggccagct tggcgaggca ccaggaggag agggcatcgg gatcggccac gccggccgcg
     6421 acgaaccagc cgtcgccatc ggggacgatg gcatgctcgt agccgccgcc ctcgaatttc
     6481 tgggcggcga gggccgcgcg ctggccggcg gagaggcgct tcagccagtc gggaaagctg
     6541 tccttgttca ccagatggat cggaatggcc tgctggccgc ggtcgggctg gatcagcgcg
     6601 tttctgtcag tcatcttaca gcaatcctga cgcgcgatag gccgggggtc gagagggaaa
     6661 atcggtcatg tcgcggtacg gattatcagc agtgctgcgc gcacagctcg cacatggcgt
     6721 ggatcagacc gcgcacgagc ggatcggaca gcgaatacca gcgggtctgc gcctcgcccc
     6781 ggatcgatac gacgccctcc tcacgcagca tggcgaggtg ctgcgaaacc gagctttgcg
     6841 acaggccggt gagggcgacc agctcgttca ccgagacctc gccctcgtcc atccggcaga
     6901 gcatcagcag gcgctcggga tggctcatca gcttgatcct gccggccatg cgggccgcgt
     6961 tcgccttcag ttcggtcagg tcggtgggct tcatcggttg tccgggatag aggtccggcg
     7021 gggcttagca gccggcctgg gtgtcggcta gcgttcgacg ggaaagcctg actgggccca
     7081 ggcgccaaag cccccggcga gatgggtatc gatctcggcg cgggcggcgc cgcatttctc
     7141 cagcgccatg cctgaccgct tgccgccctg gcagttgagc acgatggtct tgcccgcggg
     7201 gtccggcagc cggctgggct ggaaaccgga gagcggcatg ttgaccgcgc cggggatgtg
     7261 gccctgggcg aactcgccgg gttcgcgcac gtcgaccacc agcgccctgt cttccgcgat
     7321 cagcagctcc agctcggaag gggtgatctc ccgatgcgcg accttcttgc caaacccgaa
     7381 catgtccaag cctttcgtga cgacacagga ccgcgccccg atcgtcccgt actaatatca
     7441 ggcatgacga atataattca agggcaatcg aacggcaact acatcgtccg tgccggatcg
     7501 tgcccgccgc ccgattggtc gaacgcgcgg accatggctt ggac
//
LOCUS       DATNBV010000349         2886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4676153,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000349 DATNBV010000000
VERSION     DATNBV010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4676153"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VN627_08355"
     CDS             <1..185
                     /locus_tag="VN627_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HXD03304.1"
                     /translation="VDIDTALETCDILIVLVDHDLFKSIPLSERANKIVYDTRGIWPD
                     QPRARAKSAPKLKAVG"
     gene            complement(312..1115)
                     /gene="fabI"
                     /locus_tag="VN627_08360"
     CDS             complement(312..1115)
                     /gene="fabI"
                     /locus_tag="VN627_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HXD03305.1"
                     /translation="MTGLMAGKRGLIMGLANDKSLAWGIAQKLREQGAELAFSYQGEA
                     LEKRVRPLADSLGSDFLIDCDVSDMAALDTAFETLAARWPTIDFVVHAIGYSDKTQLR
                     GHFYDTTLDNFLMTMNISAYSLVAVTKRAMAMMPEGGSIVTLTYYGAEKVVPHYNVMG
                     VAKAALEASVRYLANDCGPQGVRVNAISAGPIKTLAASGIGDFRYIMKWNELNSPLRR
                     NTTIDDVGGAGLYLLSDLASGVTGEVHHVDSGYHTVGMKQEDAPDIALG"
     gene            complement(1117..2178)
                     /locus_tag="VN627_08365"
     CDS             complement(1117..2178)
                     /locus_tag="VN627_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXD03306.1"
                     /translation="MEERPIEGPGAPHPGPALAAPDLSPEARRKHAQRWAQGLEDVVE
                     GEVEAHHHLIARARQVLGRTLAGVWNDGFIHAGNLAYMGVVALFPFFITATAIFSAIG
                     ERGEREAAINAVLLAVPHSVAKAIAPVAHDVIEARTGKLLWIGGLVGLWTVGSLVETI
                     RDVLRRAYGTKATHAFWRYRLSSTAIIFAAVVLLMMSLLVQVAIGAVEQAIHAWVPEF
                     DPAFGELAMTRLIPALVLFGSLYLLFYSLTPGRYRGNGYPKWPGALFVTAWWVLVTMA
                     LPWVLRRLFNYDLTYGSLAGIMITLFFFWLVGLGMVFGAELNAALAETPEERDMVGQA
                     DNRQRDGRNRDGRMRNGGD"
     gene            complement(2188..>2886)
                     /locus_tag="VN627_08370"
     CDS             complement(2188..>2886)
                     /locus_tag="VN627_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HXD03307.1"
                     /translation="DGFEGFGGQQEGVDISDLFEGLFGGRAAGGGGGGPFGGGGGGGP
                     FGGMGGGRRAPAKGANVQYRLKVSLTDAAILAAQRITLSDGKTIDLKLPAGVEDGTQM
                     RLSGKGEHGAGGRGDAIVVIEIAPHPFFKRDGDNLRLDLPVTLDEALGGAKVKVPTAE
                     GAVMLNVAAGSSSGKTLRLKEKGMTRKDGTRGDQLVTLQIDLPPADSEAGAELAKRLG
                     DWHDTRNPRAKFGV"
BASE COUNT          495 a         1028 c          894 g          469 t
ORIGIN      
        1 tggtcgacat cgataccgca ctggaaactt gcgatatcct catcgtcctc gtcgatcacg
       61 acctgttcaa gtcgatcccg ctgtcggaac gcgcgaacaa gatcgtctac gacacccgcg
      121 gcatctggcc cgaccagccc cgagcacgcg cgaaatccgc gccgaagctc aaggccgtgg
      181 ggtaagcagg gttcacgcag aggccgcaga ggagaacggg agacgcagaa gagcagcgcc
      241 atgcggcgca gccgctctcc ctgttccttg ctgggattgc tgcgcgagcc aagccgcgca
      301 gcggctaaca atcaccccag cgcgatatcc ggcgcgtctt cctgcttcat gcccacggtg
      361 tgatagcccg aatcgacatg gtgcacctcg ccggtcacgc cgctggcgag gtcggaaagc
      421 aggtagagtc cggccccgcc cacgtcgtcg atcgtggtgt tccgccgcag cgggctgttc
      481 agctcattcc acttcatgat gtaacggaaa tcgccgatcc cgctcgccgc gagggtcttg
      541 atcggcccag ccgagatggc attcacccgc acgccctgcg gcccgcaatc attggcgaga
      601 tagcgcacgc ttgcctccag cgccgccttg gccacgccca tcacgttgta gtggggcacg
      661 accttttccg cgccgtaata ggtcagcgtc acgatcgagc cgccttccgg catcatcgcc
      721 atggcccgct tcgtcaccgc gaccagcgaa taggccgata tgttcatcgt catcaggaag
      781 ttgtcgagcg tggtgtcgta gaagtgcccg cgcagctggg tcttgtcgga atagccgatg
      841 gcgtggacca cgaagtcgat cgtcggccag cgcgccgcga gagtctcgaa cgccgtgtcc
      901 agcgcggcca tgtcggaaac gtcgcagtcg atcaggaaat cgctgccgag gctgtccgcc
      961 agcggccgca cgcgcttttc cagggcctcg ccctgatagg aaaaggcgag ttccgccccc
     1021 tgttcgcgca gcttctgggc gatcccccag gcaagcgact tgtcgttggc aaggcccatg
     1081 atgagccccc gcttgcccgc catcaatccc gtcatttcag tctccgccgt tcctcattcg
     1141 cccatcccgg ttgcgtccat cccgctggcg attgtccgcc tggccgacca tgtcgcgttc
     1201 ctccggtgtc tcggccagcg ccgcgttgag ctcagcgccg aataccatcc ctagtccgac
     1261 aagccagaaa aagaaaagcg tgatcatgat cccggcaagg ctgccatagg tcagatcgta
     1321 gttgaacagg cggcgcagca cccacggcag ggccatggtc accagcaccc accacgccgt
     1381 gacgaacagg gcaccgggcc atttgggata gccattcccg cgatagcggc ccggcgtcag
     1441 tgaatagaac agcaggtata gcgagccgaa caggaccagg gccgggatca gccgggtcat
     1501 cgccagctcg ccgaaggccg ggtcgaactc cggcacccag gcatggatcg cctgctccac
     1561 cgcgccgatc gccacctgta ccagcagcga catcatcagc aggaccacgg cggcaaagat
     1621 gatcgcggtc gaactcaggc gatagcgcca gaaggcgtgg gtcgccttgg tcccataggc
     1681 ccggcgcagc acgtcacgga tcgtctcgac cagactgccc acggtccaca ggccgacgag
     1741 cccgccgatc cacaggagct tgccggtgcg cgcctcgatc acgtcgtggg caacgggggc
     1801 gatcgccttg gccaccgaat ggggcacggc cagcagcacg gcattgatcg ccgcctcgcg
     1861 ctccccccgc tcgccgatgg ccgaaaagat cgccgtcgcc gtgatgaaga agggaaacag
     1921 cgccaccacc cccatgtagg cgaggttccc ggcatggatg aacccgtcgt tccacacccc
     1981 cgccagtgtg cgccccagca cctgccgggc gcgcgcgatc aggtggtggt gagcctcgac
     2041 ctcgccctcg acgacatctt ccagcccctg cgcccagcgc tgcgcatgct tgcgccgggc
     2101 ttcgggcgaa aggtccgggg cggcaagggc cggtcccggg tggggagcgc ccggcccttc
     2161 gatcgggcgc tcttccatgg ccggcggcta aactccgaat ttcgcgcggg gattgcgcgt
     2221 gtcgtgccaa tcgccgaggc gcttggccag ttccgctccg gcctcgctgt cagcgggcgg
     2281 caggtcgatc tgcagggtga ccagttggtc gccgcgcgtg ccgtccttgc gggtcatgcc
     2341 tttttctttc agccgcaagg tcttgccgct gctgcttccg gcggcaacat tgagcatgac
     2401 cgcgccttca gccgtcggca ccttgacctt ggcgccgccc agcgcctcgt ccagcgtcac
     2461 cggaaggtcc agccgcaggt tgtcgccatc gcgcttgaag aaggggtgcg gggcaatctc
     2521 gatcaccacg atcgcatcgc cgcgcccgcc cgcgccatgc tcgcccttgc cggacaggcg
     2581 catctgcgtg ccgtcctcaa cgccagcggg cagcttgaga tcgatggtct tgccatcgga
     2641 cagggtgatc cgctgcgcgg cgaggatggc cgcgtcggtc agcgagacct ttagccggta
     2701 ctgcacgttc gcgcccttgg ccggggcgcg ccggccaccc cccatgccgc caaagggtcc
     2761 gccgccacct ccgccgccga acgggccacc gccgccgccg ccggcggcac ggccgccgaa
     2821 gaggccctcg aacaggtcgg agatatccac cccttcctgc tggccgccga aaccctcgaa
     2881 cccgtc
//
LOCUS       DATNBV010000350         2304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3694268,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000350 DATNBV010000000
VERSION     DATNBV010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3694268"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1760
                     /locus_tag="VN627_08375"
     CDS             <1..1760
                     /locus_tag="VN627_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXD03308.1"
                     /translation="AVYFNSPTTLRAIANAGFLNTPGVVPPDGLDKAENIISLAHCGT
                     SFTCDIDLFNQVQKTDGRQITYSNFDPWASYTKIWGISNTTTIELGEHATLKNIFGYR
                     TNDSYTRGDNDGTPLQILDTDNLIQTKLITEELQLSGSLFDNKLKYTLGGFYYKQSPN
                     GPGGSAALEINTFFGLSHNASYSYFTDTSKALYGQLDYKVVDGVTLTAGYRQNWDTST
                     GCVAALTYSAFAAGIVQQDQSVIPGEAACRSGTLPTRSTAQASSSSSAVLPLAHFNKG
                     TWNLGVNWQITPDAMVYAATRRGYRAGSFNSPLYDAYLASVQTFKPETLDDIEIGAKL
                     RWRGPVSGSLDIALFRGTDKDVQIPFQTSQLVAGTAGLGCVPGADLVTASSAPLCTVT
                     SGPDAGKTGRTVPVPSATTYVNAGRSRLQGFELAATLSPTEGLTLSGGLSYVDFTALS
                     VTNDPALAAVYTANKRTVPPFLMRQQPEWTANAGIYYEYPRDVLGGRLAFNSDLRYTS
                     RWLEGDYYIEGKTTVDASIGLKGIGGTGVDASVQVINLFNAKYDYGSFGSSAALGYKS
                     TIYGQPRTVSLRLRYTFGR"
     gene            complement(1902..>2304)
                     /locus_tag="VN627_08380"
     CDS             complement(1902..>2304)
                     /locus_tag="VN627_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HXD03309.1"
                     /translation="TGGLLSAAILTVPGATGFYRGGGVIYSRRAREVVLGISARDAAG
                     MRSVTEDYALLQARGVAAQFGAEWGLAESGSAGGSVHPGGVGSGKSWAAVAGPGGAIA
                     SRLTETGSDLRAANMFAFAGAALALLAQALD"
BASE COUNT          410 a          821 c          673 g          400 t
ORIGIN      
        1 gcgcagtcta cttcaattcg ccgaccaccc tccgcgccat cgcgaacgct ggtttcctca
       61 atacccccgg cgtggttccg cctgacggtt tggacaaggc tgaaaacatc atcagcctgg
      121 cccactgcgg gaccagcttc acctgcgaca tcgatttgtt caaccaggtg cagaagactg
      181 acggtcgcca gataacctat tcgaacttcg atccgtgggc gtcctacacc aagatctggg
      241 ggatctcgaa caccaccacg atcgagctgg gcgaacatgc gaccttgaag aacatcttcg
      301 gctatcgcac caatgacagc tacacccggg gcgacaatga cggcacgccg ctgcagatcc
      361 tggatacgga caacctgatc cagaccaagc tgatcaccga ggaactgcaa ctctcgggat
      421 cgctgttcga taacaagctg aagtacacgc tcggcgggtt ctactacaag cagtccccca
      481 acgggcccgg cggctccgcg gcgctggaga tcaacacctt cttcggcctg tcgcacaacg
      541 cgtcttattc gtatttcacc gacaccagca aagccctcta cggtcagctt gactataaag
      601 tggtcgacgg ggtgacgctg accgcgggct accgccagaa ctgggatacc agcaccggct
      661 gcgtggctgc gctcacctat agcgctttcg ctgctggtat tgtccagcag gatcaatccg
      721 tcatcccggg cgaggcagcc tgccgctccg gcactctgcc gacgcggtca acggcacagg
      781 catcgagttc gtcctcggca gtccttccgc tggcccattt caacaagggc acgtggaacc
      841 tcggtgtgaa ctggcagatc acgcctgatg ccatggtcta tgcggccacc cgccggggtt
      901 accgcgccgg cagcttcaac tcgccgctct atgatgccta tctcgcgagc gtgcagacgt
      961 tcaagccgga aacgctggac gacatcgaga tcggcgccaa gctgcggtgg cgcgggccgg
     1021 tttccggcag tctcgatatc gcgctgttcc gcggcacgga caaggacgtc cagatcccgt
     1081 tccagacctc gcagcttgtc gcgggcactg ccggcctggg ctgcgttccc ggggcagatc
     1141 tcgtgaccgc ttcgtctgcg ccgctttgca ccgtgacttc gggccccgat gcggggaaga
     1201 ccggccgcac tgttccggtt ccttcggcga cgacctatgt caacgccggg cgctcgcgcc
     1261 tgcaaggctt cgaactggcc gcaaccctgt cgccaacgga aggcctgacg ctgagcggcg
     1321 gcctgtcata tgtcgacttc acggccctga gcgtcaccaa cgatccggcg ctggcggcgg
     1381 tctacacggc caacaagcgc actgttccgc cattcctcat gcgccagcag ccggaatgga
     1441 ccgccaacgc cggcatctat tatgaatatc ctcgcgatgt tctgggcggc aggctcgcgt
     1501 tcaacagcga tcttcgctac acctcgcggt ggcttgaagg cgactactac atcgagggca
     1561 agacgaccgt cgatgccagc atcggcctga aggggatcgg tggcaccggg gtcgatgcgt
     1621 cggttcaggt gatcaacctg ttcaacgcga agtatgacta cggctcgttc ggctcgtccg
     1681 ccgccctcgg ctacaagtcg acgatctacg gccagccgcg caccgtttcg ctgcgcctgc
     1741 gctacacctt cgggcgctga ccggcatggc ggcggtgccc tcgcccgggc atcgctgcca
     1801 gccatggcaa catggaaatt ccgggggccg gctgcgcgca gccggccccc gttctttcat
     1861 gttctccaga taattgccgc agtcgcccct ggcccgtggg cctagtccag cgcctgcgcc
     1921 agcagcgcca gcgccgcccc ggcgaaggca aacatgttcg ccgcacgcag gtcgctgccc
     1981 gtttcggtca accggctggc gatcgcccct cccggccccg cgaccgcggc ccacgacttg
     2041 ccggaaccga cgccacccgg atgcacgctt ccgccggccg agccgctctc ggccaggccc
     2101 cactcggccc cgaactgtgc cgccacgccc cgcgcctgca gcagcgcata gtcctcggtc
     2161 accgagcgca ttcccgctgc gtcgcgcgcg gagattccca gcacgacctc gcgcgcccgc
     2221 cgcgaataga tcaccccgcc gccgcgatag aatcccgtcg cccccggaac ggtcaggatc
     2281 gcagcggaca gcaggccgcc cgtg
//
LOCUS       DATNBV010000351         5822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1786388,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000351 DATNBV010000000
VERSION     DATNBV010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786388"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VN627_08385"
     CDS             complement(<1..1073)
                     /locus_tag="VN627_08385"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXD03310.1"
                     /translation="MPVPEGPELDRALDEANVPTLLAAYAYLSHDADFLETFAPFIRP
                     AFSYPPTEIPEDLADELRLRLRRLLTTGEGLASSEPGDALVQRIMSITVGEPVDDEFV
                     ELVYDQCGFRPWVDRSKQPDRPGPRSGFKVLVIGAGMTGLAAATKLREAGYEFVVIEK
                     NPEVGGTWYENRYPGVGVDTPSHFYSFSWEIWPDWHHYHPHGADMQRYMTGVADKYDL
                     RRDIRFNTTVQSLVWDEKACTWTVTVRKADGSTEALVVNAVINGHGPVNRLKMPDIPG
                     LADFRGPVVHTANYPADLDLKDKRVAVIGTGASSAQLCGAIAPDVAELTIYQRTKHWV
                     LYNPEIAHEVSEGMKWALVHIPT"
     gene            1251..2681
                     /locus_tag="VN627_08390"
     CDS             1251..2681
                     /locus_tag="VN627_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HXD03311.1"
                     /translation="MAISTKKTVFLTGATGTMGSATLAELARRADRFRVRALVRPDEQ
                     AHPVVKRFEGNPAVEWLVGDLTSAEDMRRGVAGVDQVLHIGGMVSPYADRFPELTMQV
                     NVGGARNIVEAIKVAGQADSTALVYIGTVAQTGHRNAPVHWGRTGDPIKISRYDHYAI
                     SKTQAEAIVAQSGLKRWVSLRQTGIAHRNMWKIFDPIIFHNPFNGVFEWVTVGDSARL
                     AANTCEDAVPETFWKQFYNIGGGEKLRVVNHEFARITARALKQADAFASYRPHWSATR
                     NFHGQWYADSDRLEAAVAFRSETLEDWAEDMVASMPALVRLIARLFPGAGRKRMEKLA
                     RGPGGTLHWFAHDDREHIEAYFGSREAWERIPREWAGFEFRQPSREVSLLDHGYDTDK
                     ADADWAASDLSSAAQFRGGAFVGGSSEPDGDAHFRCALGHEFTMSPRLYLRGGHWCPT
                     CMVDQASYDANRAANPFFAQVWPDRD"
     gene            complement(2736..4667)
                     /locus_tag="VN627_08395"
     CDS             complement(2736..4667)
                     /locus_tag="VN627_08395"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXD03312.1"
                     /translation="MTALATLQPSALPIRDQSGLRAALADANLPTLLMVYATYTQDRA
                     YLDSFAPYLTAAYTAEAPTNVPEAMARDLRDRLFALLTQPVPPREKPVDREFLCHMMG
                     VSVGEKVDPGLIPVLYDQMGFEQPAPRKDLPGRRMPPADFDVLVIGGGMSGIAAGIKL
                     SEAGYSYTIVEKNEELGGTWYENRYPGVGVDTPSHFYSYSFELNPEWSHYHPKGPEIQ
                     RYLLGVADRHGVRSNVLFNTRVTGAIWDESAAKWHVKLAHKDGTTSEKAVSAVLLAHG
                     VLNRWSMPKIAGLETFKGPMMHTAGWDPQVDLKGKRVAVIGTGASAAQLVPAIADEVS
                     QLTVFQRSKHWVLNNPDINVNVNDNVRFALRNIPHYKEWFRFRVYWFTGDGLYGNVRM
                     EADWPNKDVSISAQNDAVRNYALHYIHTKLANRPDLVEKIVPDYPIFGKRIVLDADGG
                     WLDTLVKPNVTLETRAIDHVESDAIVLKDGTRVEVDVLALATGFDIAKVAGSLDIIGK
                     DSHRIQDDWGEDDPYSYLGVMAPGYPNFFFVLGPNSAPNHAAGVNMVIEAQINWMVET
                     LDLMVAEDARTVEPTMEAYNAWNDKVAKQMSQMIWAHPKAKSYYLNSAGRNIISCPFR
                     LADYWQWTRHPARDDLVLD"
     gene            5135..>5822
                     /locus_tag="VN627_08400"
     CDS             5135..>5822
                     /locus_tag="VN627_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXD03313.1"
                     /translation="MKFGTLKFSLLIGGSMLAMFGPAQAQAQGAAAPAADAAPPSDAP
                     ASTDIIVTATRQQQRLQDVAMSVNVATGEQLQKFKIFDVKDISQLAPGLELTNTTGRN
                     NTTTLRGVTFDPDQGTGPAVRVYLDETPVDPQTAYTAIYDIQQIEVLRGPQGLLRGLS
                     APAGSITIATRKPGFDRVEGDFQATATDRAGYNVQGGVTLPFSDKLSIRVAGLVDGNR
                     LNNVRDVNRNG"
BASE COUNT         1046 a         1796 c         1905 g         1075 t
ORIGIN      
        1 aaggttggga tgtggaccag ggcccacttc atcccctcgc tcacttcgtg ggcgatctcg
       61 gggttgtaga ggacccagtg cttggtgcgc tggtagatgg tcaactcggc cacgtccgga
      121 gcaatcgctc cgcacagctg ggcggagctg gcgcccgtgc cgatcaccgc cacgcgcttg
      181 tccttgaggt caaggtcggc cgggtagttc gcggtatgaa ccaccggtcc tctgaaatcc
      241 gcaaggccgg ggatgtcggg catcttcagc cgattgaccg gaccatggcc gttgatcacg
      301 gcgttgacca cgagggcttc cgtgctgccg tcggccttgc ggacggtcac cgtccaggtg
      361 caggccttct catcccacac cagcgactgc acggtcgtgt tgaagcggat gtcgcggcgc
      421 aggtcgtact tgtcggcgac gcccgtcatg tagcgctgca tgtccgcgcc gtggggatga
      481 tagtggtgcc agtcgggcca aatttcccag ctgaagctgt agaagtggct cggcgtgtcg
      541 acccccacgc cggggtagcg gttctcgtac caggtgccgc cgacctcggg gttcttctcg
      601 atcacgacga attcgtaacc tgcctcgcgc agcttggtcg ccgcggccaa gccggtcatg
      661 ccggcaccga tgaccaggac cttgaacccg gaccttggcc cgggccgatc gggctgcttg
      721 ctgcggtcaa cccagggccg gaagccgcac tggtcgtaaa ccagctcgac gaattcatcg
      781 tccaccggct cacccacggt gatcgacatg atccgctgca ccagcgcgtc accgggttcg
      841 gacgaggcga gtccctctcc cgtggtgagc aggcgccgca ggcggagccg cagttcgtcg
      901 gccagatctt cgggtatttc ggtgggagga taggagaacg ccgggcggat gaacggcgcg
      961 aacgtctcga ggaaatccgc gtcgtgcgac agataggcat atgccgcgag cagggtgggc
     1021 acgttggctt catccagggc gcggtcaagc tcgggccctt cgggcacagg catgaacccg
     1081 ctgcgctctg cggaaagaat cgaagccatc tgaagcggtc tcccggtctg ttataagtgg
     1141 gcacttacat cataatctgt ggggaggaaa gggttttacc cgcccggttg acgttgcagg
     1201 cgattttatc gaacatatag gttcaattta ggaccgagga gcacccgcag gtggcgatat
     1261 ccacgaagaa gaccgttttc ctgaccggag ccaccggcac aatgggaagc gccacgctgg
     1321 ccgagctggc gaggcgggca gaccggtttc gggtccgtgc cctcgtccgg ccggacgagc
     1381 aggcgcatcc cgtcgtgaaa cggttcgagg gcaaccccgc ggtcgagtgg ctggtgggcg
     1441 atctgaccag cgcggaagac atgcgccgcg gcgtcgccgg ggtggaccag gttttgcaca
     1501 tcggcgggat ggtctcgccc tacgccgatc ggttccccga attgacgatg caggtgaacg
     1561 tcggcggcgc gcgcaacatc gtcgaggcga tcaaggtcgc cgggcaggcc gacagtaccg
     1621 ctctggtcta tatcggcacc gtggcccaaa ccggacaccg caacgcgccg gtgcactggg
     1681 ggcgcacggg cgaccccatc aagatcagcc gctacgatca ctacgccatc agcaagaccc
     1741 aggccgaggc catcgttgcg cagagcggcc tcaagcgttg ggtttcgctg cgccagaccg
     1801 ggatcgccca ccgcaatatg tggaaaatct tcgatcccat catcttccac aatcccttca
     1861 acggcgtgtt cgaatgggtg accgtggggg actccgctcg gctggcggcg aacacctgcg
     1921 aggacgcggt gccggaaacg ttctggaagc agttctacaa tatcggcggc ggcgagaagc
     1981 tgcgggtcgt caatcacgaa tttgcgcgga tcacggcgcg ggcgctgaag caggctgacg
     2041 cctttgcgag ctaccggccg cactggtccg caacgcgcaa tttccatggc caatggtatg
     2101 ccgattccga tcggctggaa gccgccgtgg cattccggtc ggaaacgctg gaagactggg
     2161 cagaagacat ggttgccagc atgcctgcgc tggtgcgcct gatcgcccgc ctgttccccg
     2221 gagccggacg caagcgcatg gaaaagctgg ccaggggccc cggcggtacg ctccactggt
     2281 tcgcccatga cgacagggag catatagagg cctacttcgg ttcacgcgaa gcctgggaaa
     2341 ggattccgcg cgaatgggct ggcttcgaat ttcgccagcc atcccgggag gtgtcgttgc
     2401 tggatcatgg atacgacacg gataaggccg atgccgattg ggcggccagc gatctctcgt
     2461 cggcggcgca attccgtggt ggggcattcg tcggcggatc cagcgaaccg gacggggatg
     2521 cgcacttccg ctgcgccctg ggtcatgaat ttacgatgag cccgcggctt tacctcaggg
     2581 gcggccactg gtgcccgacc tgcatggttg accaggccag ctatgatgcc aaccgggcgg
     2641 caaacccctt ctttgcccag gtctggcccg accgcgattg acccaaggcg gaggcggcgg
     2701 ttgtccgccg cctccgccgt cgatcgtgga ttggttcagt ccagcaccag gtcatcgcgc
     2761 gccgggtggc gggtccactg ccagtagtcg gccaggcgga acgggcagga gatgatgttc
     2821 cgcccggcgg agttgaggta gtagctcttc gccttcggat gggcccagat catctggctc
     2881 atctgcttgg cgaccttgtc gttccaggcg ttgtaggcct ccatcgtcgg ctcgaccgtg
     2941 cgggcgtctt cggccaccat caggtcaagc gtctcgacca tccagttgat ctgcgcctcg
     3001 atcaccatgt tgacgccggc ggcatggttg ggcgcgctgt tcgggccgag tacgaagaag
     3061 aagttgggat agcccggcgc catgaccccc agatagctgt agggatcgtc ttcgccccag
     3121 tcgtcctgga tgcggtggct gtccttgcca atgatgtcga ggcttccggc gaccttggcg
     3181 atgtcgaacc cggtggcgag cgccagtacg tcgacttcga cccgggtgcc gtccttcagc
     3241 acgatcgcat cgctttcgac atggtcgatg gcgcgggtct ccagcgtaac gttgggcttg
     3301 accagcgtat cgagccagcc gccatcggca tcgagcacga tgcgcttgcc gaagatcggg
     3361 tagtccggaa cgatcttctc gaccaggtca ggccgattgg ccagcttggt atggatgtag
     3421 tgcaacgcgt agttgcgcac ggcgtcgttc tgggcgctga tcgaaacgtc cttgttcggc
     3481 cagtcagctt ccatgcggac gttgccataa agtccgtcgc cggtgaacca gtagacgcgg
     3541 aagcggaacc actccttgta atgcgggatg ttgcgcagcg cgaagcggac gttgtcgttg
     3601 acgttgacgt tgatgtcggg gttgttgagc acccagtgct tggagcgctg gaacacggtc
     3661 agctgcgaaa cctcgtcggc gatcgcgggg accagttgcg ccgcgctcgc gccggtgccg
     3721 atgacagcga cgcgcttgcc cttgagatcg acctgcggat cccagcccgc cgtgtgcatc
     3781 atcggaccct tgaaggtctc cagcccggcg atcttgggca tggaccagcg attgaggacg
     3841 ccatgggcca gcaggacggc gctcaccgcc ttctcgctgg tggtgccatc cttgtgcgcc
     3901 agcttgacgt gccacttggc ggcgctctcg tcccatatgg cgccggtcac cctagtgttg
     3961 aagagaacat tgctgcgcac gccgtgccgg tcggccacgc ccagcaggta gcgctgaatc
     4021 tccgggccct tagggtgata gtggctccac tcggggttca gttcgaagga atagctgtag
     4081 aagtggctgg gcgtgtcgac gcccacgccg ggataccggt tttcatacca ggtcccgcca
     4141 agctcctcgt tcttttcaac gatggtgtac gagtagccgg cttccgaaag cttgatgccc
     4201 gccgcgatgc cgctcatgcc gccaccgatg accagcacgt cgaagtcggc cggcggcatg
     4261 cggcgcccgg gcaggtcctt gcgcggagcg ggctgttcga accccatctg gtcatagagt
     4321 accgggatca ggccggggtc gaccttctcg ccaacactca cccccatcat atggcacagg
     4381 aattcgcgat cgaccggttt ctctcgcggt gggaccggtt gggtgaggag cgcaaacagg
     4441 cgatcgcgca gatcgcgcgc catggcctcg ggcacattgg tcggcgcctc ggccgtatag
     4501 gccgcggtga gataaggggc gaagctgtcg agataggccc ggtcctgcgt atatgtcgca
     4561 tagaccatga gcagcgtcgg caaattggca tcggccaatg cagcgcgcag accggattgg
     4621 tcgcgaatgg gcagagccga gggctgaagt gttgcaagcg cggtcatgtc gtccatcctc
     4681 atgatcggga tgcaaaagta agtacatact tataatggcg ggtcaaggcg gttctgcaag
     4741 tccgccgacg cggtggccgt gctactgcgc gctccagatg cgtggcaaaa aagacactcg
     4801 gttacgcaaa caagcctgat tcgatcgaaa atgacgaaaa aaatgcaaaa acgatcaacc
     4861 agagtttgac ccaggtgcct tggtttgtta gtccgaacta acttcgacgc agcctgggct
     4921 atgggcgtga tcgatgggga gaggcgagcc ggatcggagc tgtgcaggct tcggtgaggc
     4981 gcaccttgtc ggctcgggat ggatccgtgc cgctctcttt cgcatccaat ggtcttgatc
     5041 ggtgtcgggg gtccacccgg ggctcagccc cgagagattt gaactgtccg gccaccgggc
     5101 gctgcggcgc caaacgtgac aaaggggagg gtatatgaaa ttcggaacgc tcaagttttc
     5161 gctgctgatc ggcggaagca tgcttgccat gttcggtccc gcgcaggcgc aggcgcaagg
     5221 cgctgcggca cccgcggctg atgccgcacc gccttcggat gctccggcga gcactgacat
     5281 catcgtcacc gcaacccgcc agcagcagcg tctccaggac gttgccatgt cggtcaacgt
     5341 tgccacgggc gagcagctgc agaagttcaa gatcttcgac gtgaaggaca tctcgcagct
     5401 cgctcccggc ctcgagctga ccaacaccac gggtcgcaac aacacgacca ccctgcgcgg
     5461 cgtgaccttc gatcccgacc agggcaccgg cccggcggtg cgcgtctatc tcgacgaaac
     5521 cccggtcgac ccgcagaccg cctacaccgc catctacgat atccagcaga tcgaagtgct
     5581 gcgcggcccc caaggcctgc tgcgcggcct ctcggccccg gcgggctcga tcaccattgc
     5641 cactcgcaag ccgggcttcg accgggttga aggcgacttc caggcgacgg ccaccgatcg
     5701 cgccggctac aacgtccagg gcggcgtgac cctgccgttc tccgacaagc tgtcgatccg
     5761 cgttgccggc ctggtcgatg gcaatcgcct caacaacgtg cgcgacgtca accgcaatgg
     5821 tg
//
LOCUS       DATNBV010000352         3791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_504943,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000352 DATNBV010000000
VERSION     DATNBV010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504943"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1066)
                     /gene="folD"
                     /locus_tag="VN627_08405"
     CDS             complement(176..1066)
                     /gene="folD"
                     /locus_tag="VN627_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HXD03314.1"
                     /translation="MTDLIDGKAFAEGLRGRVAELASAFEGKAGRKAGLAVVLVGEDP
                     ASQVYVRSKGKQTVACGMASFEHKLDAATSEADLLALVERLNADPAVDGILVQLPLPG
                     HIDEQKVIATINPDKDVDGFHVVNAGRLAVGQPGFVPCTPLGCLMLLKDRLGSLSGLE
                     AVVLGRSNIVGKPMAQLLLAESCTVTIAHSRTKDLADVVRRADIVVAAVGRAEMVKGD
                     WIKPGATVIDVGINRVPAAEEGKTRLVGDVDFASCQGIAGAITPVPGGVGPMTIAVLL
                     RNTLVAAHRNAGLALAAEAI"
     gene            complement(1080..1376)
                     /locus_tag="VN627_08410"
     CDS             complement(1080..1376)
                     /locus_tag="VN627_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HXD03315.1"
                     /translation="MLFLTLFEIVGYLINIVVTLVIVQFVLSLLISFNVVNLHNNFVA
                     AVWQAVNALLDPVLRPIRRIMPATGALDLSPMVLIIGLTILQIVLKNMAMAALY"
     gene            complement(1419..2012)
                     /locus_tag="VN627_08415"
     CDS             complement(1419..2012)
                     /locus_tag="VN627_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03316.1"
                     /translation="MTLPRQSLAQVTGALLALAGCQQHGPSAQAVAGDPLASGIAVAP
                     AAAPGSAPLSHASAPAAAVPAPETAQVSAPQVSGDRRNPDVVLREWGTAIERRDWAAV
                     RGLWGHGGADSGVGARHFAARWDRLRRPLVNIGAGVQEGAAGSLYYSAPVTITDGSRR
                     FTGTVTIRRVNDVPGASAEQLRWHADATTRAPWTTMR"
     gene            complement(2065..2970)
                     /gene="argB"
                     /locus_tag="VN627_08420"
     CDS             complement(2065..2970)
                     /gene="argB"
                     /locus_tag="VN627_08420"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HXD03317.1"
                     /translation="MTQDATRDPAMLAKAETLVEALPYLQRYAGRTFVVKYGGHAMGD
                     PELALDFAQDVVLLKAVGINPVVVHGGGPQIGAMLKKLGVESRFVDGLRVTDKATADI
                     AEMVLCGSINKELVGWIANAGGKAIGISGKDGGLVTARKVEKTMRDPDTDEMLSVDLG
                     FVGEPDVIDTTVLDTMIAAGIIPVIAPIACGTDGETYNVNADTMAGAIAAALGAARLF
                     LLTDVAGVLDKNKNLLTDLTPADIARLAEDGTISGGMIPKLETCTLAVEAGCEAAVVI
                     DGRVSHAMLIEVFTSKGAGTLIRKA"
     gene            3260..3649
                     /locus_tag="VN627_08425"
     CDS             3260..3649
                     /locus_tag="VN627_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXD03318.1"
                     /translation="MALAILTLAAFALCPEGPRTTCVVDGDTFWLAGEKIRIADINAP
                     ETHGARCASEQARGDAATRRLIALLNAGPFELAPYARDRDRYGRLLRLVVRHGQSLGA
                     QLVRERLAEPWRGRRSDWCGSVDPAGA"
BASE COUNT          575 a         1340 c         1276 g          600 t
ORIGIN      
        1 ctgggattgg catagccgcg caaggtcccc cggcccaccg cctcaccacg cggtccctcg
       61 ggccgctcct gcgcggcggc gggaacactg gcgacaaggc agaggccggc ggcgagcagg
      121 gtgcggttca tcgcgcctag ctgcccggca gcgcgtgaac ggatgctgtc cggcgtcaga
      181 ttgcctcggc cgcgagcgcg aggccggcat tgcggtgggc ggcgacaagg gtgttgcgca
      241 gcaggaccgc gatggtcatc ggcccgaccc cgcccggcac cggggtaatc gccccggcaa
      301 tgccctggca cgaggcgaaa tcgacatcgc ccaccagccg cgtcttgccc tcctcggctg
      361 ccggtacccg gttgatgccg acatcgatga cggtcgcacc cggcttgatc cagtctccct
      421 tgaccatctc ggcccggccc accgccgcca cgacgatatc ggcccggcgc accacgtccg
      481 ccaggtcctt ggtccggctg tgtgcgatgg tcaccgtgca gctttcggcc agcaacagct
      541 gggccatcgg cttgccgacg atgttggagc gccctagcac cacggcttca agaccggaca
      601 ggctgcccag ccggtccttc agcagcatca ggcagccgag cggcgtgcag ggcacgaaac
      661 caggctggcc aaccgcgagg cgcccggcat tgaccacgtg gaagccgtcg acatccttgt
      721 ccggattgat cgtggcgatc accttctgct cgtcgatatg cccgggcagc ggcagctgga
      781 cgaggatccc gtccaccgcc ggatcggcat tgagccgttc caccagcgcc agcaggtcgg
      841 cctcgctggt ggctgcgtcg agcttgtgct cgaagctcgc cataccgcag gcgactgtct
      901 gcttgccctt gctgcgcacg tagacctggc tcgccgggtc ttcgcccacc agcaccacgg
      961 caaggcccgc cttgcgcccg gccttgccct caaaggcgct cgccagctcg gcaacgcggc
     1021 cgcgcaggcc ctcggcaaag gccttgccgt cgatcaggtc ggtcacgcga tcactccgtt
     1081 cagtaaaggg ctgccatggc catgttcttg agcacgatct gcaggatcgt cagcccgatg
     1141 atcagcacca tcggcgaaag gtcgagcgca ccggtcgccg gcatgatccg gcggatcggg
     1201 cgcagcaccg gatcgaggag ggcgttcacc gcctgccaga ccgccgcgac gaaattgttg
     1261 tgcaggttca ccacgttgaa gctgatcagc aggctcagta cgaactgcac gatcaccagg
     1321 gtgacaacga tgttgatcag gtaaccgacg atttcgaaca gggtcaggaa cagcactgcg
     1381 gcgccctcgc aaatggcagg tgccgcgctc ttagccgctc agcgcattgt cgtccacggg
     1441 gcgcgggtgg tcgcatcggc atgccagcgc agttgttccg cgctggcacc gggcacgtca
     1501 ttgacgcgcc ggatggtgac cgtgccggtg aacctgcgcg aaccgtcggt gatggtcacc
     1561 ggcgcgctgt agtagagcga gccggccgcg ccctcctgca cgcccgcgcc gatgttgacg
     1621 agcgggcggc gcaggcgatc ccagcgggcg gcgaaatggc gcgcgcccac cccgctgtcg
     1681 gccccgccat gaccccacag gccgcgcacc gcggcccagt cgcgccgttc gatcgcggtg
     1741 ccccattcgc gcaggaccac atccggatta cggcgatcac cggagacttg cggtgccgac
     1801 acttgcgctg tctcgggcgc cggtacggca gcagcggggg cagacgcatg cgacagcggc
     1861 gccgagcccg gtgcggccgc gggcgcgact gctatgcctg atgccagcgg atcgccggcc
     1921 acggcctgcg cggacgggcc gtgctgctgg caaccggcaa gggccagcag ggcgccggtc
     1981 acctgcgcca gcgattggcg gggaagggtc atgctgtggg gaatcgctgc ggaaggtttg
     2041 cgttcccggc ccgcgcctgc tggctcaggc cttgcggatg agggtgccgg cgcccttgga
     2101 cgtaaacacc tcgatcagca tggcatggct gacccggcca tcgatcacca ccgctgcctc
     2161 gcagccggcc tcgaccgcca gggtgcaggt ttccagcttg ggaatcatgc cgccgctgat
     2221 ggtgccgtcc tccgccagcc gggcgatgtc ggccggggtg aggtcggtca gcaggttctt
     2281 gttcttgtcg agcacgccgg ccacgtcggt gagcaggaac agccgtgccg cgcccagcgc
     2341 cgccgcgatc gcgccggcca tggtgtcggc attgacgttg taggtctcgc catcggtccc
     2401 gcaggcgatg ggggcgatga ccgggatgat cccggcggcg atcatcgtgt cgagcacggt
     2461 cgtgtcgatc acgtcgggct cgccaacgaa gccgagatcc acgctcagca tctcgtccgt
     2521 gtccgggtcg cgcatggtct tctcgacctt gcgcgcggtg accagcccgc cgtccttgcc
     2581 ggagatgccg atggccttgc ccccggcatt ggcgatccag ccgaccagtt ccttgttgat
     2641 cgaaccgcac aggaccatct cggcgatgtc ggccgtggcc ttgtcggtca cgcgcagccc
     2701 gtcgacgaag cggctttcca cgcccagctt cttgagcatg gcgccgatct gcggtccgcc
     2761 gccgtggacc accacgggat tgatgccgac ggccttcagc agcaccacgt cctgcgcgaa
     2821 atcgagggcg agttcgggat cgcccatggc atggccgcca tacttgacca cgaaagtgcg
     2881 cccggcatag cgctgcaggt agggcagggc ctcgaccagg gtttcggcct tggcgagcat
     2941 ggcgggatcg cgggtggcgt cttgagtcat atcccgccct tagccgctcg ccgcgcaatt
     3001 tgaagcggtt ttaccgctct ggggcggcag gggcggctcc gcggctgccc ggttttttgc
     3061 ctccgatacg gcccaattgc cgcagcgcgg cccgcgaggc tcgcaccagc gcggcgggat
     3121 cggtgtccgc cgtgcgccgt cggcggcgag aattcatcca taacccactg taatccggcg
     3181 cattgtttct ccatttcgga gggaaggcag gggtttgccc ggataggaac ccgcgatcga
     3241 ttgccgttct tgccccggca tggccctcgc aatcctcacc ctcgccgcct tcgcgctctg
     3301 ccccgaaggc ccccgcacca cctgcgtcgt cgatggcgac accttctggc ttgcgggcga
     3361 gaagatccgc atcgccgaca tcaacgcgcc cgagacccac ggcgcgcgct gcgccagcga
     3421 gcaggcgcgg ggcgatgccg cgacccgccg cctgatcgcc ctgctcaacg ccggcccctt
     3481 cgagctcgcc ccctatgccc gcgaccgcga ccgctatggc cggctgctgc gcctcgtggt
     3541 ccgccacggc cagtcgctcg gcgcacagct ggtccgcgaa cggctggccg aaccctggcg
     3601 cgggcgccgg tcggactggt gcgggagcgt ggacccggcc ggggcgtgaa agccgcgcgg
     3661 cgacgaagtt gacgaagttg acacggtcct gagacagaaa accgccctgc ccgccccggc
     3721 gcctgtcccg tgggctgact ggccggcaag gcgggggcaa aagaccatcg cgccgccata
     3781 ccattcgcct g
//
LOCUS       DATNBV010000353         3997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1786714,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000353 DATNBV010000000
VERSION     DATNBV010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786714"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VN627_08430"
     CDS             <1..241
                     /locus_tag="VN627_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03319.1"
                     /translation="ELSFEIDFDPTDSAVHAKLAALQDVPAMHSWQISYPCSGTPKKA
                     TVDGFLTKFAPDAGGVQENLTAAVTVKLTGPIVWS"
     gene            246..653
                     /locus_tag="VN627_08435"
     CDS             246..653
                     /locus_tag="VN627_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03320.1"
                     /translation="MLTRISFLAPQPLERRPVTVPGLGSCFVRAMSAGERDEFEVAHA
                     KGKSRDFRARLVACTVCDEDGELVFQPADIPALSALPAATLQQLVVAATEVNRLTEGD
                     VKDLEDAAKNSPCDRNGNSSSASPLPLAARSPS"
     gene            765..1298
                     /locus_tag="VN627_08440"
     CDS             765..1298
                     /locus_tag="VN627_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HXD03321.1"
                     /translation="MRLASGATSPRTSSRWRGRGAGQPANSSRPRNRSRGYRGCSVAG
                     SIVVTGVKEIDSRMKTLERKVQRKILVQNMRKGMKLVLEDALLRVPVLSGLLKKNIKL
                     RAMKRKRNRVGLLVQIQPDPGFVKGEYWYPAAVEYGHGTVPPHPFMRPAYDLNKEHSR
                     DLAMRGMLISLEREVKT"
     gene            1423..2805
                     /locus_tag="VN627_08445"
     CDS             1423..2805
                     /locus_tag="VN627_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03322.1"
                     /translation="MTATTERLQKGLNDARSSLAGFGKSVMSIKGLIAGSFAGAGIAA
                     LGHFAGLADDLSDNVERLGTMFSDSASIVVAQAERMNKAFGTSEISFTAMAVKMGGLF
                     KSLGVAEGPAAEMASQLGVMGQALANFKGISFEQAMGKLQAGMAGKGKGLKEFGITVS
                     ATMSAQERFNAIMQGGAGILGTMDQRAMDAGASWASFRGRVEELEISLGKNLPELVEP
                     FFAELNVLVVAAGDAFKGLKQTTLDWGEGSLEAAKSSAEGMGWVQKAVGGVADAWQFM
                     AGVFHTWQANFTGSIAAIVDALIDLDKWLEKNLPELLGGGKKGENRDFLEGFSASLHE
                     SADKQFEDLQKEWAKPWSSEGIDAAFAASKKKIEEARKEVTKGALAAATPAAKATAGS
                     TLAKSAGEALTIGSSGAASALIRSKYDIGDEGHNKKMAKNSDEQVKLLGGIKAALEAG
                     DDEPEIMDDI"
     gene            2808..3788
                     /locus_tag="VN627_08450"
     CDS             2808..3788
                     /locus_tag="VN627_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03323.1"
                     /translation="MQIKSITDVRGNRSVSWSGQGGRTYSQQVRVITDDPTMGMRAVA
                     KALSFRVGDSYIHPLTTTPTEWDYGSYLQGFDLKEEGDDGKQWLCTLNYGQFNWAEQG
                     GATTEAASDGQTSPFSVPPKVSFGSARYERECHLDNKGKAIVNTVGDPYDPPLKRDDS
                     RGTLTIVRNEPTFNSQYVQTFKDTCNQDVFLAIYAPNTVKCADVVAEREYHADYGYYW
                     VVTYQFEIREFVTDASNNVIYAGWTEMIPNSGLHELKTPFDPTQGKKPIILGGAPATK
                     PVLINQNGVYAPGADPYYLKFQLYPLQDFSKFNFADDLLTVSSVPGGGGS"
     gene            3792..>3997
                     /locus_tag="VN627_08455"
     CDS             3792..>3997
                     /locus_tag="VN627_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03324.1"
                     /translation="MPDSKPKGPVYLSKPLARRFGRAVKRVEAMYGRPPDQRSSSPVN
                     PFLPIQPAQVTTAIPTGTLTAPSSS"
BASE COUNT          805 a         1248 c         1270 g          674 t
ORIGIN      
        1 cgagctgtcc ttcgagatcg acttcgaccc gaccgatagc gccgtccacg ccaagctcgc
       61 ggccctgcaa gacgtcccgg cgatgcactc gtggcagatc agctacccgt gctcgggcac
      121 gcccaagaag gccacggtcg acggcttctt gaccaagttc gcgccggatg ccggcggcgt
      181 gcaagagaac ctcaccgcgg cggtcaccgt caagctgacc ggccccatcg tctggagttg
      241 accccgtgct cactcgcatc tcgttcctcg ccccgcagcc cttggagcgg cgccccgtca
      301 cggtacccgg cctcggctct tgcttcgtcc gcgccatgtc ggccggcgag cgggatgaat
      361 tcgaggtcgc ccacgccaag ggcaagtcgc gcgacttccg ggcccgcctg gtcgcctgca
      421 cggtctgcga tgaagacggc gagctggtct tccagcctgc cgacatcccg gccctgtcgg
      481 cattgcccgc cgccacgctg cagcaactgg tcgtcgccgc caccgaggtc aaccgactga
      541 ccgagggcga cgtcaaggac ctggaggacg cggcaaaaaa ctcaccttgc gaccgcaacg
      601 gcaattcctc ttccgcctcg cccttgccct tggccgcacg gtcgccgagc tagaagcctc
      661 gctttccgac cgcgagctcg aggaatggct cgcgttcaac cgccacgtct gcccgctgcc
      721 cgacccgtgg tcgatcgggg cggggctctg ggcggtgctc gccaatgcgt ttggcaagcg
      781 gcgctacaag cccgaggact tcatcccggt ggcgcggtcg cggcgcaggc cagccggcga
      841 acagctcacg gccgaggaat cggtcgcgtg gctacagggg gtgctcggtg gcgggatcga
      901 ttgtggtgac gggggtgaag gagatcgact cgaggatgaa gaccctggaa aggaaggtcc
      961 agcggaagat cctcgttcag aacatgcgca aggggatgaa gctggtcctg gaagatgcgc
     1021 ttttgcgggt gccggtcctg tcgggcctgc tcaagaaaaa catcaagctc cgggcgatga
     1081 agcggaagag gaaccgcgtc gggctgctgg tgcagatcca gcctgatccg gggttcgtca
     1141 agggcgaata ctggtacccc gcggcggtcg agtacgggca tggtacggta ccaccccatc
     1201 cgttcatgcg accggcctat gacctgaaca aggagcactc gcgcgacctc gccatgcgcg
     1261 gcatgctgat cagcctcgag cgagaagtca aaacataatg gcattagtgg gtacgatcaa
     1321 cgacgggcag tccgatcggg aggcttgaat agtcatgctg ttgatttcga ttaacattcg
     1381 tgtaaggaca tatcaatctt agtcggtacg atcgcaattg ccatgacggc cacgacggag
     1441 cgcttgcaga agggcctgaa cgatgcgcgc agttcgctcg ccgggttcgg caagtcggtc
     1501 atgtccatca agggcctgat cgccggcagc ttcgcgggcg cggggatcgc ggccctgggg
     1561 cacttcgccg ggcttgcaga cgatctttcg gacaatgtag agcgcctcgg cacgatgttt
     1621 tccgatagcg catccattgt ggttgcgcaa gcggaacgca tgaataaagc ctttggtacg
     1681 agcgaaattt ccttcaccgc gatggcggtg aagatgggcg gcctcttcaa atctctcggc
     1741 gtggcggaag gtccagcggc tgagatggcc tcgcaacttg gcgtgatggg gcaggccctg
     1801 gctaatttca aagggattag cttcgagcag gccatgggca agctacaggc cggcatggcg
     1861 ggcaagggga aggggctgaa ggaattcggg atcaccgttt cggcgacgat gagcgcgcag
     1921 gagcggttca acgcgatcat gcagggcggg gccggcatcc tgggcacgat ggaccagcgc
     1981 gcgatggacg cgggggcttc ctgggcctcg ttccgcgggc gcgtcgagga gctggagatt
     2041 tccctcggca agaacctccc cgagctggtc gagccatttt tcgccgagct caatgtgctg
     2101 gtggtcgcgg cgggcgatgc gttcaagggc ctcaagcaaa cgaccctcga ctggggcgaa
     2161 ggcagcctgg aagccgcgaa gtcatcggcc gagggcatgg gctgggttca gaaggcggtc
     2221 ggcggcgtgg ccgatgcctg gcaattcatg gccggggtct tccacacctg gcaagcaaac
     2281 ttcaccggct cgattgcggc gatcgtcgat gcgctgatcg acctcgataa atggctggag
     2341 aagaacctgc ctgaattgct cggcggcggc aagaaagggg agaaccgcga cttcctggag
     2401 ggcttctccg ccagcttgca cgagtcagcc gacaagcaat tcgaagacct gcaaaaggaa
     2461 tgggccaagc cctggtcttc cgaaggcatc gacgccgctt tcgccgcctc gaaaaagaag
     2521 atcgaggagg ctcgcaagga agtcaccaag ggcgctctcg cagccgccac gccagccgcg
     2581 aaagcgacgg ctggttccac gctcgccaaa tcagccggcg aggccctcac gatcggcagt
     2641 tccggtgccg ccagtgccct gatccgcagc aagtacgaca tcggcgacga agggcacaac
     2701 aagaaaatgg ccaagaactc tgatgagcag gtcaaactgc tgggcggtat caaggcggcg
     2761 ctggaagccg gcgatgacga gcccgagatc atggacgaca tctaggcgtg caaatcaaat
     2821 ccattaccga cgtgcgcggc aaccgctccg tctcctggtc gggccagggc gggcggacct
     2881 actcgcagca ggtccgggtg atcacggatg acccgacgat gggcatgcgt gccgtggcca
     2941 aggcgctcag cttccgggtc ggcgatagtt atatccaccc cctgaccacc acgcccacgg
     3001 aatgggatta cggttcctac ttgcagggtt tcgatctcaa agaagagggc gacgacggca
     3061 agcaatggct ctgcacgctc aactacggac agttcaactg ggccgaacag ggcggggcca
     3121 cgaccgaagc ggccagtgac ggccagacca gcccgttcag cgtgccgccc aaggtctcct
     3181 tcgggtcggc caggtacgag cgggaatgcc atcttgacaa caagggcaaa gccatcgtca
     3241 acacggtcgg cgatccctac gacccgccgc tcaaacgtga cgactcgcgc ggcacgctca
     3301 cgatcgtgcg caacgagccg accttcaatt cgcagtatgt gcagacgttc aaggacacct
     3361 gcaaccagga cgtgttcctc gccatctatg caccgaacac ggtcaagtgt gccgatgtcg
     3421 tcgccgagcg ggagtatcac gccgattacg gctattactg ggtcgtcacc tatcagttcg
     3481 agatccgcga attcgtgacc gatgccagca acaatgtgat ctatgccggc tggacggaga
     3541 tgatacccaa ttcgggattg catgaattga aaacgccgtt cgaccccaca cagggcaaaa
     3601 aacccatcat cctgggcgga gcaccggcga cgaaaccagt cctgatcaac cagaacggcg
     3661 tctatgctcc cggggccgat ccttactacc tgaaattcca gctctatccg cttcaagact
     3721 tcagcaagtt caacttcgct gacgacctgc tcacggtatc aagcgtgccg ggcggaggcg
     3781 gatcatagcc ggtgcccgac tccaagccca aaggtccggt ctatctcagc aagcctctgg
     3841 cgcgccgctt cggccgggcg gtcaagcggg tggaggcgat gtacggccgc ccgcccgacc
     3901 agcgctcaag ctcgccggtt aatcccttcc tgcccatcca gccggcccag gtcacgacgg
     3961 ccattcccac gggcacgctc accgctccct cgagctc
//
LOCUS       DATNBV010000354         3370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2124919,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000354 DATNBV010000000
VERSION     DATNBV010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124919"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VN627_08460"
     CDS             <1..363
                     /locus_tag="VN627_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03325.1"
                     /translation="GSDTPFRTPGGGGDGPSSGGDAAVTVARLETHLAWVKKGLGGLA
                     VVSLGALAWIFYNIYQPMQVLVKDSAVQTQILAGMNDDISKIGDRLDRIYDHSQAIPG
                     VRQTEPPQSGKGAASRKP"
     gene            complement(495..1118)
                     /gene="folE"
                     /locus_tag="VN627_08465"
     CDS             complement(495..1118)
                     /gene="folE"
                     /locus_tag="VN627_08465"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HXD03326.1"
                     /translation="MSSIDGPDEDGFDPVGADGKIVVPEHVQEAIRTLIRWSGDDPGR
                     EGLLDTPRRAARAWKEYCQGYGEDPAIHLARIFEEVGGYNEVVLLKGIPFQSHCEHHM
                     APIIGTAAIAYLPRNHVVGISKLARVLHGFARRLQIQERLTAEVAQCIWDNLKPLGVA
                     VVIEASHACMTARGVRTPGVNMVTSRMMGTFLEDERSRKEVLSLMGY"
     gene            complement(1134..2654)
                     /locus_tag="VN627_08470"
     CDS             complement(1134..2654)
                     /locus_tag="VN627_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXD03327.1"
                     /translation="MDPDSGPAAGMDDEEGAAAQPSVWRYAMATRAHVVVDAAEYFLL
                     MQEAMLRARQRIMLIGWDFDTRVRLGPGRRWWNRPTRRRHPARLGPFVVWLANRNRAL
                     QVRVLKWNFGALKFIFRGTMIFDLIRWFRHPAIDFKFDSAHPLGCSHHQKIAVIDDRF
                     AVCGGIDMTTERWDTPEHREHDPRRVNASRRPYDPWHDATMLLEGDAARALGDLGRSR
                     WRRAGGPRLEPCTPQEATAWPSRTTAQFENVEVGISRTTAKWGGYDEVREVEALFLEH
                     IAAARHFIYAESQYFASRRVAEALAARLSEPDPPEVFLVGPLVAHGWLEQTAMDGARV
                     RLLHAIGERDPQRRMRVFVPYSGAKPIYVHAKIMIIDDVILRVGSANLNNRSMGLDSE
                     ADVVIDSRRPANDRDEIRRAIRDLRLQLLGEHCGIPADTVAALLDEHGSMAAMADARP
                     LSSKRLEPFNLRPLGDTEKALADSALLDPESPGELFEPMSRRAGLFRRGGLLRRPR"
     gene            complement(2846..>3370)
                     /locus_tag="VN627_08475"
     CDS             complement(2846..>3370)
                     /locus_tag="VN627_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXD03328.1"
                     /translation="YVNAGRSRLQGFEIDGTLSPVEGLTLSGGLSYTDFTALSLTPDP
                     NLAAVYTANGRRSPPFVMRQQPEWIANAGIYYEYPRDILGGRLAFNSDLRYTSSWVEA
                     DFPIPGKTTVDASIGLKGIGGTGLDASVQVINVFNKFYEYGSAGSAASLGFRSTILGQ
                     PRTVSLRLRYTFGK"
BASE COUNT          609 a         1124 c         1068 g          569 t
ORIGIN      
        1 ggcagtgata cgccgttccg aacacctgga ggcggagggg acggcccatc gtcaggcgga
       61 gatgcagctg tgaccgtggc gaggctcgaa acccacctcg catgggtaaa gaaaggtctt
      121 ggcggtctcg ctgtggtgag cctcggcgca ttggcttgga tcttctacaa catctaccag
      181 cctatgcagg ttctggtgaa agactccgcc gtgcagacgc aaatccttgc tgggatgaat
      241 gatgacatct ccaagattgg cgaccgattg gatcgaattt acgaccactc tcaagcaatc
      301 ccgggagtaa ggcaaactga accacctcaa tcagggaagg gagcagcaag ccgtaaaccc
      361 tgacgcggcc cattctgccg ccgaaccaat agcccgaaag tctcaagaat gtccgaaagt
      421 tcgagggtgc ctagcgacac cctccgacca tgcggcccaa aacaacttcc cgccgcgctt
      481 gggaaccgcc ccgcttagta acccatcagg ctgaggactt ccttgcggct ccgctcatcc
      541 tcaaggaacg tgcccatcat ccggctggtc accatgttga cccccggcgt gcgcacgccg
      601 cgtgcggtca tgcaggcatg gctggcctcg atcaccacag ctacgcccag cggcttgagg
      661 ttgtcccaga tgcactgggc gacctccgcc gtcagccgct cctggatctg caggcggcga
      721 gcgaagccgt ggagcacgcg ggccagcttg gagatgccga ccacgtggtt gcgcggcagg
      781 taggcgatgg ccgccgtgcc gatgattggc gccatgtggt gttcacagtg cgactggaag
      841 gggatgccct tgagcaggac gacctcgttg tagccgccca cttcctcgaa gatgcgcgcc
      901 aaatggatcg ccggatcctc gccatagccc tggcaatatt ccttccacgc gcgcgccgcg
      961 cggcgcgggg tatcaagcag gccctcgcgc cccggatcgt cgcccgacca gcggatcagg
     1021 gtgcggatgg cttcctggac atgctcgggc acgacgatct tgccgtcggc gcctacggga
     1081 tcgaagccgt cctcatcagg tccatcgata ctgctcaagt cggtattcct cttctatcgt
     1141 ggccggcgga gcaagccccc gcgtcggaac aggccggcgc ggcggctcat cggctcgaac
     1201 agctctcccg ggctctccgg atcaaggagc gcgctgtcgg ccagtgcctt ttcagtatcg
     1261 ccaagcggac gcaagttaaa tggctccaat cgcttggagc tcagcggacg ggcgtcggcc
     1321 atggccgcca tcgatccgtg ctcatccagc agggcggcca ctgtgtcggc cggaatgccg
     1381 cagtgctcgc ccagcagttg cagacgcaaa tccctgatcg cacggcgaat ttcatcgcgg
     1441 tcattggccg ggcggcggct gtcgatgacc acgtcggcct cgctgtccag tcccatcgaa
     1501 cggttgttca ggttagccga gcccacgcgc aatatcacat catcgataat catgatcttg
     1561 gcgtgtacgt agatcggctt ggccccggaa tacggcacaa agacccgcat ccgacgctgc
     1621 ggatcgcgct cgccgatggc atggagcagg cggacacggg ccccgtccat ggcggtctgc
     1681 tcgagccagc cgtgggccac gagtgggccg accaggaaca cctccggagg atcgggctcc
     1741 gaaagccttg ccgccagggc ctcggccacc ctgcgcgagg cgaagtactg gctttcggca
     1801 tagatgaagt gacgcgcggc ggcgatgtgt tcgagaaaca gggcctcgac ctcgcgcact
     1861 tcgtcatagc cgccccactt cgcggtggtc cgcgagatgc ccacttcgac attttcgaac
     1921 tgggcggtgg tccggctcgg ccaggccgtc gcctcctgcg gggtgcacgg ctcgagccgg
     1981 ggcccgcccg cgcggcgcca gcgcgagcgg ccgagatcgc ccagcgcccg ggcggcatcg
     2041 ccctccagca gcatggtcgc atcgtgccac gggtcgtaag gccggcgcga ggcgttgacc
     2101 cggcgggggt cgtgctcgcg gtgctcgggc gtgtcccatc gctcggtggt catgtcgatc
     2161 ccgccgcaca cggcgaaacg atcgtcgatc accgcgatct tctggtgatg gctgcagccc
     2221 agcggatgag cggaatcgaa cttgaaatcg atcgccgggt ggcggaacca gcggatcagg
     2281 tcgaagatca tcgtcccgcg aaagatgaat ttcagcgcgc cgaaattcca tttcagcacc
     2341 cgcacctgca gcgcccggtt gcggttggcc agccagacca cgaacggccc cagccgcgcc
     2401 gggtggcgcc ggcgggtcgg ccggttccac cagcgccgcc ctggccccag ccgcacccgc
     2461 gtatcgaagt cccagccgat cagcatgatc cgctggcgcg cccgcagcat cgcctcttgc
     2521 atcagcagga aatattcggc cgcgtcgaca acgacatggg cgcgcgtggc catcgcatag
     2581 cgccagaccg acggttgcgc cgccgcgccc tcctcgtcgt ccatgcctgc tgccggcccg
     2641 ctgtcaggat ccatcgccgc tcccaccttg gcagcctaga cgggactgcg tgccggggaa
     2701 acccgcactg gcggatggcg gttccctcgc gccgctccac aaacagaaag ggcggcgcct
     2761 tgcggcaccg ccctttcttt tattcaacca tggtccgacc ttgctcgagg cggcgcccgc
     2821 caggcgggca ccgctccggc ctggctcact tcccgaaggt gtagcgcagg cgcagcgaca
     2881 cggtgcgcgg ctggccgagg atcgtcgagc ggaagcccag cgaggccgcc gaaccggccg
     2941 aaccgtattc gtagaacttg ttgaacacgt tgatcacttg aaccgaggca tcgagcccgg
     3001 ttccaccgat ccccttgagg ccgatgctgg catcaaccgt cgtcttaccc gggatgggga
     3061 agtcggcttc cacccagctg gacgtgtagc gcagatcgct gttgaacgcg agccggccgc
     3121 ccagaatatc gcgaggatat tcatagtaga tcccagcatt ggcgatccac tcgggctgct
     3181 ggcgcataac gaacggaggg gatctgcgac cattggcggt gtagacggcc gcaaggttcg
     3241 ggtccggcgt caggctcaac gccgtgaagt cggtgtagga cagaccaccg ctcagggtca
     3301 ggccctccac tggcgacagt gttccatcga tctcgaaccc ttgcagacgc gaacgaccgg
     3361 cgttgacgta
//
LOCUS       DATNBV010000355         3114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2763369,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000355 DATNBV010000000
VERSION     DATNBV010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2763369"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VN627_08480"
     CDS             complement(<1..1060)
                     /locus_tag="VN627_08480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXD03329.1"
                     /translation="MTGATSARPLRVLSLATLYPHDGAPNFGVFVARQMEAVAGRGDV
                     ELTVISGRGLPPFPLSLHPRYRALAALPEAEERGGVAVRRPRFRVIPGIGRFNAGALA
                     RAVLPLARRLHDDRPFDLIDAQFFYPDGPAAVRIGATLGLPVSIKARGADIHHWGMRG
                     DTGPQVLAAGRAAAGLLAVSQGLADDMAALGMPAERIAVHRTGLDRAVFRPLDRAECR
                     VRLGLPADAPVLACVGALIERKGQRFAIDALGALPGAVLVLAGAGPDEAALRAQAERL
                     GLADRVRLLGAVPHGELPVVLSAADVFVLPSASEGLANAWVEALACGTPVVTTPTAGA
                     RELLTDAAYGRFAGRNGAE"
     gene            complement(1057..1632)
                     /locus_tag="VN627_08485"
     CDS             complement(1057..1632)
                     /locus_tag="VN627_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXD03330.1"
                     /translation="MINRIRDIRKEKGLTLADVAARCSPPTTAQTIGRLETGMRQLSL
                     TWMNKIASALGVEPQLLVRGEEAAEPKIVARLSESGAEPLSTPRDAILPSQLAEDGPM
                     IALAIEAASGEYKAGDQLWLRQTSDEQGWTRLVNRDVLVPRPGGRFAFGRLIDRDGRR
                     VAILPPGRGQRQVIIDNPAWLAVAEMLVRPL"
     gene            1733..2200
                     /locus_tag="VN627_08490"
     CDS             1733..2200
                     /locus_tag="VN627_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HXD03331.1"
                     /translation="MRRELVERELTITGPGRGGPRKGRRSVTVNLAESPVTWLHAHGH
                     IDDRQLAAGECLRRDFEAAQLSPSLTMRWEPVRLSGGGVRMNATERQLAAKDRFDGAI
                     ASAGSGLADILWRVVCSGESLAVAERALAWPARSGKLVLRFALDRVAAFYRVP"
     gene            complement(2714..>3114)
                     /locus_tag="VN627_08495"
     CDS             complement(2714..>3114)
                     /locus_tag="VN627_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HXD03332.1"
                     /translation="DRTVTVTLGLDGSGKVDVVAGGYADWERQRKARSAPTKAAPKAA
                     EAAPPPPPPPTKKAKLSYKDQRDYDTLPDRIAELDVAIARGEAQLADPDLYARDPQKF
                     AAIMAALDKVRKDKDAAEERWLELAEQVEG"
BASE COUNT          494 a         1108 c          995 g          517 t
ORIGIN      
        1 tctctgcgcc attccgcccg gcgaaacgcc cataagccgc atcggtcagc aattcccgcg
       61 cgcccgcagt cggagtggtt accaccggcg tcccgcaggc gagcgcctcc acccaggcat
      121 tggccagccc ctcgctggca cttggaagca cgaacacgtc cgccgccgaa agcaccaccg
      181 gtagctcgcc gtgcggcact gccccgagca gccgcacgcg gtcggcaagc cccaatcgct
      241 ccgcttgcgc ccgcagcgcc gcctcgtccg gtcccgcacc ggccaggacc agtaccgccc
      301 ccggcagcgc accgagcgca tcgatggcaa accgctgccc cttgcgctcg atcagcgcgc
      361 ccacgcaggc cagcaccggc gcgtccgccg gcaagcccag ccgcacccgg cattcggcgc
      421 ggtccagcgg acgaaacacc gctcggtcca gcccggtgcg gtgcaccgcg atccgctctg
      481 ccggcatgcc cagcgccgcc atgtcgtcgg ccaggccctg ggaaaccgcc aggagccccg
      541 ccgccgcgcg cccggcagcc agcacctgcg gccccgtgtc gccccgcatt ccccagtggt
      601 gaatgtccgc gccgcgcgcc ttgatcgaca ccggcagccc gagcgtcgcg ccgatccgca
      661 ccgccgccgg cccatcgggg tagaagaact gcgcatcgat caggtcaaat ggccggtcgt
      721 catgcagccg ccgagccagc ggcagcaccg cgcgggcgag cgcccccgca ttgaaccgcc
      781 cgatcccggg gatcacccga aaccggggtc ttcgcacagc caccccgccg cgctcctccg
      841 cttccggcaa ggcggccaga gcccggtagc gtggatgcag cgaaagcgga aagggcggca
      901 gcccgcgccc gctgatcacc gtgagctcca cgtccccgcg tccggcgaca gcctccatct
      961 gcctggccac gaacaccccg aaattgggcg cgccgtcatg cggataaagc gtggcgaggc
     1021 tgagcacccg caacgggcgg gcgctcgtcg cgccggtcat aggggccgca ccagcatctc
     1081 ggccacggcc agccaggccg gattgtcgat gattacctgc cgctgcccgc gcccgggcgg
     1141 aaggatcgcc acgcgccgcc cgtcgcggtc gatcagccgt ccgaacgcaa agcggccgcc
     1201 ggggcggggg accaggacat cgcggttgac cagccgggtc cagccctgct cgtcgctcgt
     1261 ctggcgcagc cacagctggt cgcccgcctt gtattctccg ctcgccgcct cgatggcgag
     1321 cgcgatcatc ggcccgtcct cggccagctg ggagggcagg atcgcgtcgc gcggtgtgct
     1381 cagcggctcg gccccgctct ccgacaggcg ggccacgatc ttgggctcgg ccgcctcctc
     1441 gccgcgcacc agcagttgcg gctcgactcc cagggccgac gcgatcttgt tcatccaggt
     1501 cagcgatagc tggcgcatac cggtctcgag ccggccgatg gtctgcgccg tggtgggcgg
     1561 cgaacagcgc gcggcgacgt cggccagggt taggcccttt tccttgcgga tgtcgcggat
     1621 gcgattgatc atctggaagc tccttcgcgt gtaaccatat tggttttttc gctttcctac
     1681 aagcatggcc gcttggcact ccggcgcggc aatcacgaga aggggcgaat cgatgcggcg
     1741 cgaactggtt gaacgcgaac tcaccatcac cgggccgggg cggggcggcc cgcgcaaggg
     1801 gcggcgcagc gtcacggtca acctagccga aagcccggtg acctggcttc acgctcacgg
     1861 ccacatcgac gaccgccagc ttgccgccgg cgaatgcctg cggcgcgatt tcgaagcggc
     1921 ccagctcagt ccctcgctaa ccatgcgttg ggaaccggtg cgcctgtccg gcggcggtgt
     1981 ccgcatgaac gcgaccgaac gacagcttgc cgccaaggac cgcttcgacg gcgccatcgc
     2041 cagtgccggg tccggcctcg ccgatattct ctggcgcgtg gtctgctcgg gtgaaagcct
     2101 ggccgtggcc gaacgggcgc tggcctggcc ggcgcgcagc ggcaagctgg tgctcagatt
     2161 tgcgctggac cgggtagcgg ctttttatcg ggtgccgtga ggacgggctg cagagcgcgt
     2221 ccccgatttg tatcggtccc ttgtcgattt ctcgcatcgt ggtcgaaaat gaaatattct
     2281 gtgatcttgc cgcatgaaga tatataatac gatgcgccaa atggcacgcg tattgtacca
     2341 tgcgtgaaat ttgcgacgtc gatttggaat gcatctggat gatcgagagt ggtatactct
     2401 gccaatactg atttttcaga tgttctggcg cagcactccg catctatctt gaatgcggcg
     2461 tctcgactga gtccgtaacg gttttctaag tatgtgcgga aggcatgcac tgctcggtcc
     2521 agtgcttccc tgtctgttag cggcgggtgc ccagaacaca tccgcgacgg caagctgcac
     2581 gcggcgactc caagtgcgac cgcgataaga gtcacgctcg caaagacctt catcgcttgc
     2641 tccgttgggc catcactgcg gatgccatct ggcacgctag tggtgaaaat gtcccctggc
     2701 taacaaagta taattacccc tccacctgct ccgccagttc gagccagcgt tcctcggccg
     2761 cgtccttgtc cttccggacc ttgtccagcg ccgccatgat cgccgcgaac ttctgcggat
     2821 cgcgggcgta gagatcggga tcggccagct gcgcctcgcc gcgcgcgatg gccacatcca
     2881 gttccgcaat ccggtcaggc agcgtgtcgt agtcgcgctg gtccttgtag gagagcttgg
     2941 ccttcttcgt cggcggtggt gggggaggcg gtgcggcctc ggctgccttg ggcgcagcct
     3001 tggtgggggc ggagcgcgcc ttgcgctgcc tttcccaatc ggcatagccg ccggcgacga
     3061 cgtcgacctt gcccgaacca tcgagcccga gcgtaactgt caccgtgcga tcaa
//
LOCUS       DATNBV010000356         3784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3412713,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000356 DATNBV010000000
VERSION     DATNBV010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3412713"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VN627_08500"
     CDS             <1..373
                     /locus_tag="VN627_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HXD03333.1"
                     /translation="VLAITSHLPHLIAYTIVGTASDLEMVTESEVIKYSAGGFRDFTR
                     IAASDPTMWRDVFLSNKEAVLEMLQRFTEDLTALQRAIRVGDGDQLFDHFTRTRAIRR
                     GIIEQGQDDARPDFGRSDHAG"
     gene            complement(518..1789)
                     /locus_tag="VN627_08505"
     CDS             complement(518..1789)
                     /locus_tag="VN627_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HXD03334.1"
                     /translation="MTTSPPVDSPRRRRWYSVLGPGLVTGAADDDPSGIGTYSQVGAQ
                     FGYTQPWALLFSLPLMAAIQEICARIGAGTGLGIAQNLKEHYPRALLRIVVLLLLVAN
                     VINLGADLGAMGAAVALIVPGPALAATIVFGIVSILLEVFVSYARYAAVLKWATLSLF
                     SYFAVVLLAEVDWYAALRGTLVPHFAFTKDHVTALVAVLGTTISPYLFFWQSGQEVEE
                     QHRRHVKPLMVSPRSAGPELRRIRVDTLVGMACSNLTAIVIVIATAATLHKAGITQID
                     SAVEAASALRPLAGELAFALFALGIVSTGLLAVPVLAGSAAYAVAEMFGWREGLNQRP
                     REAKAFYAVIAVATLAGVAMSFLAINPMKALYWAAVVNGLLAPPLMIVTMLIARNPKV
                     MGELTISRGLAFWGWLSTGVMGAVAVLFMAV"
     gene            complement(1786..2466)
                     /locus_tag="VN627_08510"
     CDS             complement(1786..2466)
                     /locus_tag="VN627_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXD03335.1"
                     /translation="MRSLAFYLLFYPGTVLLLSLIAAQLALPGRAFIRSIGWWTGYHR
                     WCVRRLLGIRITIEGEVPSGAVLVAMKHESFFEAIDLPELLPYPAVFAKIELMRLPVW
                     GKAGARYGLIGVERDQGARALRAMVGAARQRVAEGRLLAIFPEGTRVRHGCSPPLQSG
                     FAGLYKLLGLPVVPAAVDSGRLYHSTIKRKGTITVRFAPAIEPGLPRAEIEARVHAAI
                     NALNEGDR"
     gene            complement(2508..3038)
                     /locus_tag="VN627_08515"
     CDS             complement(2508..3038)
                     /locus_tag="VN627_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HXD03336.1"
                     /translation="MIRRTVSTLLLVYVLGFLWFGIFLPAPAGPARTDAVVVLTGSGG
                     RIGRGIEVLNAGLAPRMLVSGVDREVRPAEFAAQYGLPMRLLNCCITLGYESVDTRSN
                     ARESADWIAAHHIGSVRLVTSDWHMRRAALDLAAVAPPGVTVIEDAVVTRPTFYVLFL
                     EYHKLLARLAQRVLGL"
     gene            complement(3049..>3784)
                     /locus_tag="VN627_08520"
     CDS             complement(3049..>3784)
                     /locus_tag="VN627_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HXD03337.1"
                     /translation="VQIIEARADERDRQARAALAALARTPGVSEAHIVPQAELDALVE
                     PWLGDGAVGAAGDPGAGAVPVPALIDARLAGKLSAQRLAELQAALRGAAPGARVDAQS
                     TWLSPVFGAIASLQWLSLALIVFLSLATAAAVLLAARTALGNNRATIEIVHLLGGTDA
                     QIARIFQRSIARDAIGGALAGTGAALVVILLLGRSFAALQAGIVTGGTLRVADWALLP
                     VIPVAAVVLATLTARWTVIRALKGML"
BASE COUNT          663 a         1280 c         1337 g          504 t
ORIGIN      
        1 cgtcctcgcc atcaccagtc atctgccgca cctgattgcc tatacgatcg tcggcaccgc
       61 gtccgatctg gagatggtga ccgaaagcga ggtcatcaaa tattcggccg gcggcttccg
      121 cgatttcacc cgcattgccg cctccgatcc gacgatgtgg cgcgacgtgt tcctgtcgaa
      181 caaggaggcc gtgctggaaa tgctccagcg cttcaccgaa gacctgacgg cgctccagcg
      241 cgccattcgg gtgggcgatg gggaccagct gttcgaccac ttcacccgca cccgcgcgat
      301 ccgccgcggg atcatcgagc aggggcagga cgatgcccgg ccggatttcg ggcggagcga
      361 tcacgccggc taattttcct cgcttgcgga gagggggacc gccgccgcag gcggtggtgg
      421 agggggttct cctccgcagc gcggcgctta cctgaacgcc ccctccgtca gccctggcag
      481 gctgccacct ccccgcgagc ggggcggatt ttcatcttca aaccgccatg aacagcaccg
      541 ccactgcgcc catcaccccg gtgctcagcc agccccagaa ggcaaggccg cgcgaaatcg
      601 tcagttcgcc catcaccttg ggattacggg cgatcagcat ggtcacgatc atcagcggcg
      661 gggccagcag gccgttgacc acggcggccc agtagagcgc cttcatcggg ttgatggcga
      721 ggaagctcat ggcaacgccg gcgagggttg cgacggcgat gaccgcgtag aacgccttgg
      781 cctcacgcgg gcgctggttg aggccctcgc gccagccgaa catctcggcc acggcatagg
      841 ccgccgagcc tgccagcacc ggcacggcca gcaggccggt actgacgatc cccagcgcga
      901 acagggcgaa ggccagttcc cccgcgagcg gacgcaaggc cgaggcagcc tcgaccgcac
      961 tgtcgatctg ggtgatccct gccttgtgca gcgtcgccgc cgtggcgatg acgatgacga
     1021 tcgcggtgag gttggaacaa gccatgccga ccagggtatc cactcggatc cggcgcagct
     1081 ccggcccggc gctgcgcgga ctgaccatca gcggcttgac gtggcggcga tgctgctcct
     1141 ccacctcctg gcccgattgc cagaagaaca ggtagggact gatcgtcgtc cccagcacgg
     1201 cgacaagcgc cgtcacatgg tccttggtga aggcgaaatg cgggaccagc gtgcccctga
     1261 gggccgcgta ccagtccacc tcggcgagca ggaccacggc gaagtaggag aacagcgaaa
     1321 gcgtcgccca cttgaggacg gcagcgtagc gcgcatagga aacgaagacc tccagcagga
     1381 tgctgacaat gccgaagacg atcgtcgccg ccagcgccgg gccgggcacg atcagggcca
     1441 cggcagcccc catggcgcca agatcggccc cgaggttgat cacgttggcc acgagcagca
     1501 acaggaccac gatccgcagc agggcgcgcg gatagtgctc cttgaggttc tgggcgatgc
     1561 caagcccggt ccccgcgccg atccgcgcgc agatttcctg gatcgcggcc atcagcggca
     1621 ggctgaacag caatgcccaa ggctgggtgt agccgaactg cgcgccgacc tggctgtagg
     1681 tgccgatgcc gctgggatca tcatcggccg caccggtgac gaggccgggg ccgaggaccg
     1741 agtaccagcg ccgacggcgg ggcgagtcga cgggtgggct ggtggtcatc ggtcgccttc
     1801 gttgagcgcg ttgatcgcgg cgtgtacgcg cgcctcgatc tcggcgcggg gcagcccggg
     1861 ttcgatggcc ggcgcgaagc gcaccgtgat cgtgcccttg cgcttgatcg tggagtgata
     1921 gagccgcccg gaatcgaccg cggccggcac cacgggcagg cccagcagct tgtacagccc
     1981 ggcaaagccc gattgcagcg gggggctgca cccgtggcgc acgcgggtgc cctccgggaa
     2041 gatcgccaga aggcggccct cggccacgcg ctggcgagcg gcgccgacca tggcgcgcag
     2101 ggccctcgcg ccctggtcgc gctccacccc gatcaggccg tagcgagcgc cggccttgcc
     2161 ccagaccggc aggcgcatca gctcgatctt ggcgaagacg gcgggatagg ggagcagctc
     2221 gggcaggtcg atcgcctcga aaaagctttc gtgcttcatc gccaccagca cggcgcccga
     2281 gggcacttcg ccctcgatcg tgatacgaat tcccagcagg cgcctgacgc accagcggtg
     2341 atagccggtc caccagccga tcgagcgaat gaaggcgcgg cccggcagcg ccagctgggc
     2401 ggcgatcagg ctgagcagga gcacggtgcc cggatagaac agcaggtaga aggccaggct
     2461 gcgcacaacg tcgccggggg tcgcacgcgg ctgcctcgcg atctccgtca caggccgagc
     2521 acccgctgcg cgagccgggc gagcagcttg tggtattcga ggaataggac ataaaacgtc
     2581 ggccgggtaa cgacggcatc ctcgatcacg gtgacgcccg gaggtgccac ggcggcgaga
     2641 tcgagggccg cgcggcgcat gtgccagtcg ctggtgacga ggcgcaccga gccgatgtga
     2701 tgggcggcga tccagtccgc cgattcgcgg gcattgctgc gggtgtcgac cgattcgtag
     2761 ccaagcgtga tgcagcagtt cagcagccgc atcggcaggc catattgcgc ggcgaattct
     2821 gccgggcgca cttcccggtc cacgcccgaa acgagcatgc gcggggccag gccggcattg
     2881 agcacctcga tcccgcgccc gatcctcccg cccgagccgg tgaggacgac gacagcgtcc
     2941 gtcctggccg gaccggccgg cgccggcagg aatatcccga accagagaaa gccgagcaca
     3001 tagaccagga gcagggtcga gacggtgcgg cggatcatcg gcgcaggctc acaacatgcc
     3061 cttcagtgcg cggatcaccg tccagcgcgc ggtcagcgtg gcgaggacga ccgcggcgac
     3121 aggaatcacc gggagcaggg cccaatcggc tacgcgaagc gtgccgccag tgacgatgcc
     3181 ggcctggagc gcggcgaagc tgcggccaag gaggaggatc acgaccagcg ccgcgccggt
     3241 tccggcgagc gcgcccccga tagcgtcgcg ggcgatcgaa cgctggaaga tccgggcaat
     3301 ctgggcatcg gtgccgccga gcaggtggac gatctcgatc gtggcgcggt tgttgccgag
     3361 cgcggtgcgc gccgcgagca ggaccgccgc cgcggttgcg agcgaaagaa acacgatcag
     3421 cgcaagggag agccattgga gcgaggcgat ggcgccgaag accgggctca gccaggtcga
     3481 ctgcgcatcg acccgcgcgc ccggcgccgc cccccgcaag gcggcctgga gctcggccag
     3541 gcgctgggcg gaaagcttcc cggcaaggcg cgcgtcgatc agggcgggga ccggtacggc
     3601 ccctgccccc gggtcgccgg cggcacccac cgcgccgtcg ccgagccagg gttccacgag
     3661 ggcatcgagt tcggcctggg ggacaatgtg cgcctcgctc actccgggcg ttcgggccag
     3721 ggccgccagg gcggcgcggg cctggcggtc acgctcgtcg gcacgggcct cgatgatctg
     3781 gacg
//
LOCUS       DATNBV010000357         2656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4040197,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000357 DATNBV010000000
VERSION     DATNBV010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4040197"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VN627_08525"
     CDS             complement(<1..726)
                     /locus_tag="VN627_08525"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXD03338.1"
                     /translation="MKAKAILALSACAMSLVMTDAAWAQDAGQAAQTANSDSNGMSGD
                     IVVTARRRAEDIAKVPISITAFSSQQLITKNIVNVSDLTKVTPGLNITGGGSRTNVFI
                     TIRGQSRGVTGNVSPGVLTYFNEVPLPTYGSVIPTFDMDNIQVLKGPQGTLFGRNSIG
                     GAVLTYSKAPSYDFNGYVKGEIGQYANRTGEAAVNLPIIPEHVALRVAGQLSHIGGYT
                     KSYLYNPYVLTGGINPGGNTIASI"
     gene            1048..1353
                     /locus_tag="VN627_08530"
     CDS             1048..1353
                     /locus_tag="VN627_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03339.1"
                     /translation="MVDARSMVLAVTVCADTTDQHLVRQVRVPVRDEPGAPGRGEHPP
                     ACPFSSLSALGLAAETPAPFAAALAFALVLGLAALRLPTPRRWVRLRPPLRGPPVLA"
     gene            1457..2488
                     /locus_tag="VN627_08535"
     CDS             1457..2488
                     /locus_tag="VN627_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXD03340.1"
                     /translation="MTRSLYLALLGGALAAVCSGPALADHGAAGTGASASGPLIGASA
                     ATMAEGKAAISLELTWARPDNRSDAELAALAGRHIHAHDQDHGEVYQLGLSYGLTDDL
                     TLSASLPYVRRIAIREGAHSHVGGQAIDSVVDRGDAIGFGDAALLAKWRFTGEHHHGW
                     EAALLAGIKLPTGSTGQVDDLGETFETEHQPGSGSWDPILGLAATRPLPRGSIDLSVM
                     YQIATRGAQDTRLGDRAQASVGLTWRLWGAPIDYHGGHHHHDTAEGRPTLDGVIELNG
                     EWEGRQNESGTIDVYSGGKALYLSPGLRLTAGGWSFALGGSVPLAQDIALSHPPVGYR
                     ARFAIGRAL"
BASE COUNT          427 a          901 c          896 g          432 t
ORIGIN      
        1 gatcgaagcg atggtgttgc caccgggatt aatgccaccg gtcagcacat aggggttgta
       61 caggtaggac ttggtatagc cgccgatgtg cgaaagctgg cctgcgacgc gcagcgcgac
      121 atgctcggga atgatcggaa ggtttaccgc ggcttccccc gtgcggttgg cgtactggcc
      181 gatctcgccc ttgacgtagc cgttgaaatc gtaggacggc gccttcgagt aggtcagcac
      241 cgcgccgccg atcgagttgc ggccgaacag cgtgccctgc ggccccttga ggacctggat
      301 attgtccatg tcaaaggtcg ggatcaccga accataggtc ggcaaaggaa cttcgttgaa
      361 ataggtcagc acgccgggcg agacgttgcc ggtcacgccg cgcgactggc cgcggatagt
      421 gatgaacacg ttggtgcgcg aaccgccgcc agtgatgttg agacccggcg tgaccttggt
      481 caggtcagac acgttgacga tgttcttggt gatgagctgc tggctggaga aggcggtgat
      541 cgaaatcgga accttcgcga tatcttccgc ccggcggcgg gccgtcacga cgatatcgcc
      601 gctcatgccg ttgctgtcac tgttcgcagt ctgcgcagct tgcccagcat cctgggccca
      661 cgcggcatcc gtcatcacca gcgacatcgc gcaggcggac agtgcaagaa tagccttggc
      721 cttcatagta ccctccctgt ttgcggccag gcgtttccct ggtctgcagc agttcatact
      781 aaggcataac gtagcagttg caaccatttc gctcattcca tcgcgtctat gcacgaccag
      841 tgaagcgcat tgccgcggcg cggggacccc gaaggccagc catgttggca aatggaaaaa
      901 atcaccagcc ggtggagaca agcgccgcag ccctgctaag gggcgcgggt gactgcgttc
      961 cgggccttgc tagctccaca gcggagactg tccctttggc tcgccctggc gtgccttgcg
     1021 gttcgcgcgc tcgttcccgc cggatacatg gtggacgcgc ggtcgatggt ccttgcggtc
     1081 accgtctgcg ccgataccac ggaccagcat cttgtccggc aggtcagggt gccggtgcgc
     1141 gacgaaccgg gcgcacccgg gcggggcgag catcccccgg cctgtccgtt ctccagcttg
     1201 tccgccttgg gactggcggc agaaaccccc gccccgttcg cggccgcgct cgcctttgcg
     1261 ctggtcctcg ggctggcggc attgcggctg ccgacgccgc ggcgctgggt gcggctgcgt
     1321 ccgccgctgc gcgggccgcc cgttctcgcc tgatcgactg aacagcaggg ccggccggcc
     1381 gaacgcgggc cgtcgccctc agccccggac gaacctgggc gcatcttccg catgcgcccg
     1441 actgcgagaa tttgacatga ccagatccct ttaccttgcg ctgctcggcg gcgcgcttgc
     1501 tgccgtgtgc tccggcccgg cccttgccga ccacggcgcc gccggcaccg gagccagcgc
     1561 ctcgggcccg ctgatcggcg cctctgccgc gaccatggcc gaaggcaagg ccgcgatcag
     1621 cctggagctg acctgggcca ggcccgacaa ccgcagcgat gccgagctgg ccgccctggc
     1681 cggacgacac atccatgctc acgaccagga ccatggcgaa gtctaccagc tgggcctgtc
     1741 ctatggcctg accgacgatc tcacgctctc cgcctcgctg ccctatgtgc gccgcatcgc
     1801 gatccgcgag ggagcgcatt cgcatgtggg cggccaggcc atcgacagcg tggtcgaccg
     1861 cggcgacgcg atcgggttcg gcgacgctgc gctgctggcc aagtggcgct tcaccgggga
     1921 gcatcatcac ggctgggaag ccgcgctcct ggcggggatc aagctgccga cgggatcgac
     1981 cgggcaggtc gacgacctgg gcgagacgtt cgagaccgag caccagcccg ggagcggcag
     2041 ctgggatcca atcctcggcc tggccgcgac ccgccccttg ccgcgcggca gcatcgatct
     2101 cagcgtgatg taccagatcg ccacgcgcgg ggcgcaggac acccgccttg gcgaccgcgc
     2161 ccaggcctct gtcggcctga cctggcggct ctggggggcg ccgatcgact accacggcgg
     2221 ccaccaccat catgacaccg ccgagggaag gccgaccctg gacggcgtga tagaactcaa
     2281 cggcgaatgg gaaggccgcc agaacgagag cgggacgatc gacgtctaca gcgggggcaa
     2341 ggcgctgtac ctttccccgg ggctgcgcct gactgccggc ggctggtcgt tcgcgctggg
     2401 cggcagcgtg ccgctggcgc aagacatcgc cttgtcgcac ccgccggtag gctatcgcgc
     2461 gcggttcgcg atcggccggg cactgtagca gcctgatgcg gcggccccgg gagaccaggg
     2521 ccgccgcatt ggctctgccg ccccgcacgc atcaccaccc ggacccgcgc ggggagagtg
     2581 gggggaaaat cgccgggggg cacaaatcag catggacccg atcatcgccc cctcaagggt
     2641 tcacggcctg gcgggg
//
LOCUS       DATNBV010000358         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2129280,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000358 DATNBV010000000
VERSION     DATNBV010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129280"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1556
                     /locus_tag="VN627_08540"
     CDS             186..1556
                     /locus_tag="VN627_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
                     /protein_id="HXD03341.1"
                     /translation="MAEAPLRPASFAHQAGIQPSGAGPYHQVVLPLAVYQGASRPDLG
                     DLRVFNGQGEVLSHALLRSEVQAESTRREVAVPFFPLSVPGPTAAGEGDVAVTVRQGT
                     DGTLVSVQRSAAGTKSGTVIRGVVVDASQLPGSIRSLRLLTAPSAVPLHRYTIESSPD
                     LQGWRPLKRDAQLVHLEHRGHRIDSDTVEWDSPADRYLRLLWADPGQAPEVSAVRLGV
                     VETSFRQPLRIWSADIAPASVKGGIYDYAWAGQLPLDKLRINLPQVNTLAPLAIQRQV
                     TRLSRHRQRAEAHWETLTRTVVYRLQSPQGEVRSDDIALHAGVENRLRLSLDAQGGAI
                     GSEPPKLQIGFVPHVLVFLARGEGPFVLAWGASSVERADLPAATLMPGYDGRGQLSAT
                     PASLAALESLAGPSAAPAGKAAADDGAVPARWILWAVLGVGLLVLGAMARALTRQLRQ
                     DPAAKP"
     gene            1692..>2403
                     /locus_tag="VN627_08545"
     CDS             1692..>2403
                     /locus_tag="VN627_08545"
                     /inference="COORDINATES: protein motif:HMM:NF019874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HXD03342.1"
                     /translation="MRLTADEKTVPRIHLIGTLTVVLFLTLGLAAFFSWQNLGERRAS
                     IARLERAGQEIVEARLKSEMQSAVSFIEFSRQRTEDVLRASISEQVDIAMQIAQGIYD
                     REAPRRPAADVKRLIVEALRPVRFYEGRGYYFIDDMAGQFVLLPTAPQFEGKTALDNR
                     DDTGHYIMRGLIEAAGKPRGEGFSRYRWYKPDDPQRMADKLAYVRHFAPYDWLIGTGD
                     YTYQWEAAQRKEVIARLRV"
BASE COUNT          370 a          833 c          808 g          392 t
ORIGIN      
        1 ggcagtctcg aacgcatcgt ctccttcatc gtggtcggtc tgctgctgtt gctagtcggc
       61 tacttcgccc ccttgccccc ccggtcgccc gcggacgatg gcgaggcggg cgcttcaacc
      121 gccagcgagg cgccatgaaa acgtcgatcg ccctgatcct cgccgccctg ctcgccccgg
      181 ccgcggtggc ggaagcgccg ctccgcccgg cgagcttcgc ccaccaagcc ggcatccagc
      241 ccagcggagc cgggccttat caccaggtgg tcttgccgct ggcggtctat cagggcgcca
      301 gccgtcccga tctcggcgat ctgcgggtct tcaacggcca gggcgaggtc ttgtcccatg
      361 ccttgctgcg cagcgaggtg caagccgagt cgacgcgccg cgaagtggcc gtcccgttct
      421 tcccgctgtc ggtgcccgga ccgacagccg ccggggaggg cgatgtcgcg gtgaccgtcc
      481 gccagggaac cgacgggacg ctggtttcgg tgcagcgctc ggcggccggt accaagtccg
      541 gcacagttat ccgcggcgtg gtggtcgatg ccagccagct gcccggcagt atccgttcgc
      601 tgcgcctgct gacggcgccg agcgcggtcc cgctccaccg ctacacaatc gagagcagtc
      661 cggatctgca aggctggcgg ccgctcaagc gcgacgccca gctcgtgcat ctggaacatc
      721 gcggtcaccg gatcgatagc gacaccgtcg agtgggacag tccggccgac cgttatctgc
      781 ggctgctctg ggccgacccc gggcaggccc cggaggtcag cgcggtgcgg ctgggcgtgg
      841 tcgaaaccag cttccggcaa ccgctgcgca tctggtccgc ggacatcgcg ccggccagcg
      901 tgaagggcgg catctacgac tacgcctggg cggggcaatt gccgctcgac aagctgcgca
      961 tcaatctgcc gcaggtcaat acgctggccc cgctggccat ccagcgccag gtaacccgtt
     1021 tgagccgcca ccgccagcgc gccgaggcgc attgggaaac cttgacccgg accgtcgtct
     1081 atcggctgca atcgccgcag ggcgaggtcc gctcggacga catcgcgctc cacgccggcg
     1141 tcgagaatcg tctgcgcctg agcctggatg cccagggcgg ggcgatcggc agcgagccgc
     1201 ccaaattgca gatcggtttc gtgccgcatg tcctggtctt cctggcgcgc ggcgaggggc
     1261 ctttcgtgct cgcctggggc gccagtagcg tggagcgcgc cgacttgccg gcggcgacct
     1321 tgatgccggg ctacgatggc cgtggccaat tgagtgcgac accggccagc ctggcggcgc
     1381 tggagagtct ggccgggccg tcggcagcgc cggccggcaa ggccgcggcg gacgatggcg
     1441 cggtgccggc ccgctggatc ctgtgggcgg tgctcggcgt cgggctgctc gttctcggcg
     1501 ccatggcccg ggcgctgacc cggcaattgc gccaggatcc cgccgcgaag ccttgatcgc
     1561 ttccagaagg cggtttttgg ccgcgcaagc gccattccat cagggtattt gcgggtagcg
     1621 gccctgattc ggcgatgtcc acggctagaa tggcgggaaa ttagccgaat cgccgaaaga
     1681 atcaaatagc cgtgcgcctg accgccgacg aaaaaaccgt tccgcgtatc cacctgatcg
     1741 gtaccttgac ggtcgtcctc ttcctgacgc tggggctggc cgctttcttc tcctggcaga
     1801 acctcggcga acggcgggcc tcgatcgcgc gcctcgaacg cgccggccag gaaatcgtcg
     1861 aagcccgcct gaagtccgaa atgcagagcg cggtgagctt catcgagttc tcccgccagc
     1921 gcaccgaaga tgtcctgcgc gccagcatca gcgagcaggt cgatatcgcc atgcagatcg
     1981 cccaaggcat ctacgaccgc gaggcgccgc gccggccggc cgccgacgtc aagcgcctga
     2041 tcgtcgaggc gctgcgtccg gtccgtttct acgaggggcg gggctactat ttcatcgacg
     2101 acatggcggg ccagttcgtc ctcctgccga cggcgcccca gttcgaaggc aagacggctc
     2161 tcgacaatcg cgacgatacc ggccactaca tcatgcgcgg cctgatcgag gcggcgggca
     2221 aaccgcgggg cgaagggttc tcgcgctatc gctggtacaa acccgacgat ccgcagcgga
     2281 tggccgacaa actcgcctat gttcgccact ttgctcccta cgactggctg atcggcaccg
     2341 gcgattacac ttaccaatgg gaggcggccc agcggaaaga ggtgatcgcc cgcctgcgcg
     2401 tgc
//
LOCUS       DATNBV010000359         2040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3703656,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000359 DATNBV010000000
VERSION     DATNBV010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3703656"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(618..1493)
                     /locus_tag="VN627_08550"
     CDS             complement(618..1493)
                     /locus_tag="VN627_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD03343.1"
                     /translation="MSDRLKGKVAVITGAASGIGLATVELFIEEGARVLAADVQEDAG
                     HALEQRFPGVLRFARCDVTEPPQILQAIDSAAEHFGGLDILFSNAGAVGSLAGVENWD
                     AAAWDATHALLLRSVVAGAAYAVPHMKRRGGGAIVNTSSISALQAGYAPLAYSVAKAG
                     VLHFTRLAATELSAHRIRINAVVPGFIATSIFGGIFNLGLEGSRQMAAQIAERSGSAN
                     PIGRSGLPRDIAEAALFLASDAAGFVTGTYLTVDGGITIGPRHAWDPKTPSPMTDAMG
                     ISQEQLQALRKARQA"
     gene            complement(1612..>2040)
                     /locus_tag="VN627_08555"
     CDS             complement(1612..>2040)
                     /locus_tag="VN627_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanone monooxygenase"
                     /protein_id="HXD03344.1"
                     /translation="LQGRDGLSIQEKWRPGALNYLGLAVAGFPNLFNIAGAGSTSAFT
                     SVIQSIEHHVNWICECIAWLDTQRLTTIEATVHAEADWMAHVQAMAEQTVFLSCNSWY
                     MGANVPGKPRLFMPLTGGFPAYMARCAQVATNGYDGFRVS"
BASE COUNT          370 a          669 c          658 g          343 t
ORIGIN      
        1 cgagggctgg cgccagggaa gggcatcgca gcaggcgcga tctggccgct ctcggtcgtg
       61 aacgaggcgg cggactctac gcggtgcctc gggggcctcg tcctcgagtc acattgagca
      121 acgacaaggc ttctgactag aaaggttgtg cgaccaacca acgaggtgtc accctaacgc
      181 gggcgctagc ggtcggacct tgtttgaagc agaaacttct ccttgattcc gggtcgaggt
      241 tctattccac cgttgcgacc gagcgcgcga ccggggtcct ttcattagat gatgggggga
      301 actcgatgac ctcgagggcg agaacttggt gctcgtcggg cgaggcgcgg aaggcaacaa
      361 ccagcggtct tccgttgcgt tgaccggttg aatccgccgt cgcgagttcc acttcaccgt
      421 ctgcgagagc agccgctagc cgacgcccgg cgtgaccgca aaatgcctgc catcaaactc
      481 ctgtccggcg aatgaagttc caaggcatcg cggacacggg ggccatggat cgcttagcga
      541 ccggtgtcgg caacggctgt cattgagttg cgggacttga gcggcagcac atggagacgg
      601 aggtagcctc atcaatgtca cgcttggcgc gccttgcgca aggcttggag ttgctcttgg
      661 ctgatgccca tcgcatcggt catcgggctg ggcgtcttag ggtcccaggc atggcgcggg
      721 cctatcgtga tgccgccgtc caccgtgagg taggtgccgg tgacgaagcc tgccgcgtcg
      781 gacgccagga acagcgccgc ctcggcgatg tcccgcggca accccgagcg cccgatcggg
      841 ttggccgagc cgctacgttc ggcgatctgc gccgccatct ggcgcgagcc ttccaagccc
      901 aaattgaaga tgccgccgaa gatgctggtc gcgatgaagc ctggcacgac cgcgttgatg
      961 cggatccggt gcgccgacag ttcggtggcc gccagccgcg tgaagtgcag cacgccagcc
     1021 ttcgccaccg aataggccag tggtgcgtag ccggcctgca gcgccgatat cgacgaggtg
     1081 ttgacaatgg cgccgccgcc gcggcgcttc atgtgcggca cggcgtaggc ggcgccagca
     1141 accaccgagc gcagcaagag cgcgtgggtg gcatcccagg ccgccgcgtc ccaattctca
     1201 accccggcca gcgagccaac ggcgccggcg ttgctgaaca ggatatctag tccaccgaaa
     1261 tgttcggccg ccgaatcgat cgcctgcagg atctgcggcg gctcggtcac gtcgcagcgg
     1321 gcaaaccgca gcacgcccgg gaagcgctgc tcaagcgcgt ggccggcgtc ttcctgcaca
     1381 tccgcggcga gcacgcgcgc accctcctcg atgaacagct ccacagtggc cagaccgatg
     1441 cccgacgctg cgccggtgat gacggccacc ttccccttga gacgatctga cacgctttat
     1501 cccccttcat ccgaaatggc atcggcatcg cgcgccgcct tgcaaccggc gcctcccgtt
     1561 cgcgaggcgc tcagcgatgc cctcaagaag cgcccgtgag aggccgaggc atcaactcac
     1621 cctgaacccg tcatagccgt tcgtcgcaac ctgagcacag cgtgccatgt aggccggaaa
     1681 gcccccggtc aagggcataa acaggcgtgg cttaccggga acgttcgccc ccatgtacca
     1741 ggagttgcaa ctcaggaaca cggtctgctc ggccatggcc tgcacgtggg ccatccagtc
     1801 cgcctcggca tgtacggtgg cctcgatcgt ggtcaggcgc tgcgtgtcga gccaggcgat
     1861 gcactcgcag atccagttca catgatgctc gatcgactgg atcacgctgg tgaatgcaga
     1921 tgtgctaccg gccccggcga tgttgaacag attcggaaac ccagctacgg cgaggcccag
     1981 gtagttcaac gcccccggcc gccacttctc ctggatcgac aggccgtcgc ggccctgaag
//
LOCUS       DATNBV010000360         2148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3414127,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000360 DATNBV010000000
VERSION     DATNBV010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3414127"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="VN627_08560"
     CDS             <1..1419
                     /locus_tag="VN627_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HXD03345.1"
                     /translation="AGAMVGAVVIQWISSRALALIVPVLLLSVAVYTIFSPRMDDADS
                     HARLGERGYLPLAGGIGFYDGFFGPGTGQFFAVSLVGLRGLGLTRATGLAKLFNVTSN
                     VASLIAFGLGGKVLWLLGLCMAVGAMTGGWLGAHFAARHGARLIRPLLILASLGLTGR
                     LIWSCFGKPGGGYSKGYYTEDLPGPAKGAQAAWHAARGWIFVAIDHLGVGDSSLHSPE
                     KLPFVPVVAGNNAAEQEVLRRLGEGTLAPAFPKVANPLKLGIGQSMGGYATILQQGRY
                     HGYDGIGVLGYSAIHTHPPVRPGEPPIVKPLIPRDPFAPTGELVVLNAEQVAAAPGSD
                     EDGPRMAWGFHWDDVPADLQKMDLLRFQQSRIARNTRSDDCPAWGSDTFPGVMAGQTL
                     CPGAVTPEAAAVRVPVLCAMGERDVIADPWAEPRAFKCSPSVDLFVCPRMGHMHNFAS
                     TRELFWEKIDLWGRWVGIQAGR"
     gene            complement(1425..>2148)
                     /gene="queA"
                     /locus_tag="VN627_08565"
     CDS             complement(1425..>2148)
                     /gene="queA"
                     /locus_tag="VN627_08565"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HXD03346.1"
                     /translation="EIAFPAGVSATAEARHPDGSWTLLFAGEEPVEVLLERAGRMPLP
                     PYIAGKRPTDEADKADYQTMFAREEGAVAAPTAALHFTPDLIAALAEAGIGHETLTLH
                     VGAGTFLPVKADDTEDHAMHAEWGRIEPDVAERLNAVRARGNRIIAVGTTSLRLLESA
                     AGENGMIRPFAGDTSIFITPGYRFRCIDGLMTNFHLPKSTLFMLVSALMGLERMQQAY
                     AHAIAQGYRFYSYGDASLLLPG"
BASE COUNT          301 a          719 c          750 g          378 t
ORIGIN      
        1 gccggcgcga tggtgggggc cgtggtgatc cagtggatct cgtcgcgcgc cctggcgctg
       61 atcgtgccgg tcctgctgct gtcggtcgcg gtctatacga tcttctcgcc gcggatggac
      121 gatgccgaca gccatgcgcg ccttggtgag cggggatatc tgccgctggc cggggggatc
      181 gggttctatg acggcttttt cggccccggc acggggcagt tcttcgcggt ctcgctcgtc
      241 ggcctgcgcg ggctgggcct gacccgcgcg acgggcctgg ccaagctgtt caacgtgacc
      301 agcaatgtcg ccagcctgat cgccttcggc cttggcggca aggtgctgtg gctgctggga
      361 ctgtgcatgg ccgtgggcgc aatgaccggc ggctggctgg gcgcccactt cgccgcacgg
      421 catggcgccc ggctgatccg tccactgctg atcctcgcca gccttggcct gaccgggcgc
      481 ctgatctgga gctgcttcgg caagcccggc ggcggttact ccaagggcta ctacaccgag
      541 gatctgcccg gccccgccaa gggcgcccag gccgcatggc atgctgcgcg cggctggatc
      601 ttcgtcgcca tcgatcacct cggcgtgggc gacagcagcc tgcattcacc cgagaagctg
      661 cccttcgttc cggtcgtcgc cggcaacaat gccgccgaac aggaggtgct gcgccgcctc
      721 ggcgaaggca ccctcgcccc cgcttttccg aaggtggcca atccgctcaa gctgggcatc
      781 ggccagtcga tgggcggcta tgccacgatc ctccagcagg gccgctacca cggctacgac
      841 ggcatcggcg tgctcggcta cagcgctatc cacacccatc ccccggtgcg gccaggcgaa
      901 ccgccgatcg tgaaacccct gatcccgcgg gatccgtttg cgccgaccgg cgaactggtc
      961 gtgcttaatg cggaacaggt tgccgccgcc ccgggcagtg acgaagacgg cccgcgaatg
     1021 gcctggggct tccactggga cgacgttccg gccgacctgc agaagatgga ccttctccgc
     1081 ttccagcaat cgcggattgc ccgcaatacc cggtctgacg attgcccggc ctggggttcc
     1141 gacaccttcc cgggcgtcat ggccgggcag acgctgtgcc ccggggcagt tacccccgag
     1201 gccgccgccg tgcgtgttcc cgtcctctgc gccatgggcg agcgcgacgt gatcgccgac
     1261 ccgtgggccg agccgcgcgc cttcaagtgc tcgccctcgg tcgacctgtt cgtctgcccg
     1321 cgcatgggcc acatgcacaa cttcgccagc acccgtgagc tgttctggga aaagatcgat
     1381 ctatggggcc ggtgggtggg aattcaggcg ggtcgctgac cggttcagcc gggcagcagc
     1441 aggctggcat ccccgtagct gtagaaccgg tagccctgcg caatcgcgtg ggcataggct
     1501 tgctgcatcc gttccagccc catcagcgcg ctaacaagca tgaatagcgt gcttttcggc
     1561 aggtggaagt tggtcatcag gccatcgatg cagcggaagc ggtagccggg ggtgatgaag
     1621 atcgacgtgt cgcccgcgaa gggtcggatc atgccatttt cccccgcagc gctttcgagg
     1681 aggcgcagcg aggtggtgcc cacggcgatg atccggttgc cgcgggcgcg caccgcgtta
     1741 aggcgctcgg ccacgtccgg ttcgatccgg ccccattcgg cgtgcatcgc gtggtcttcg
     1801 gtatcgtccg ccttgaccgg gagaaaagtg ccggcgccga cgtggagggt cagcgtttcg
     1861 tggccgatgc cggcttcggc gagagctgcg atcagatcgg gggtgaaatg gagcgcggcc
     1921 gtgggcgcgg cgacggcgcc ttcctcgcgc gcgaacatgg tctggtaatc ggccttgtcc
     1981 gcctcgtcag tcggtcgctt gccggcgatg tagggcggca gcggcatgcg gccggcgcgt
     2041 tcgaggagga cttcgaccgg ctcctcgccc gcgaacagca gggtccagct gccatcggga
     2101 tggcgcgcct cggccgtggc gctgacgccg gcggggaagg cgatctcg
//
LOCUS       DATNBV010000361         7847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3043311,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000361 DATNBV010000000
VERSION     DATNBV010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3043311"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..353)
                     /locus_tag="VN627_08570"
                     /pseudo
     CDS             complement(1..353)
                     /locus_tag="VN627_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013159.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(582..1868)
                     /locus_tag="VN627_08575"
     CDS             complement(582..1868)
                     /locus_tag="VN627_08575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXD03347.1"
                     /translation="MHHARITAERTGPPLRLLLLLGNWTGGGAERVAVHLMNGLPALG
                     HALAPLAGAPPLDVHLGLLHDGQAWRDQLDPARVHVAPEAARFGFDRPNRELLNPATL
                     ATGAALAPGAIRRIIAQVRPDVVMSFLKGTAILTWRALAGMPASARPRWIIREGNNIL
                     ATARQESPGRLVQAASLALTGMAYRTADAILANARGMAGDLLGLLGLDPGRVHLIHNP
                     VDIAAIEQAASIPAPDLPARPYILAVGRLEPQKGHDLLLEAFARSGMASSHELVILGQ
                     GSRHAALCAQAAGLGIAERVHFVGFTANPHAWMAAADLFVLPSRWEGFPNAAAEAMAA
                     GAPLLLADCDYGSADLVADGVNGTLVPPGDAVAMAHAIRRLLANPAHRAALAAEGRRS
                     VHRFARDRILARYARLVAEVAAVQPAPAASLRFIRA"
     gene            1986..3512
                     /locus_tag="VN627_08580"
     CDS             1986..3512
                     /locus_tag="VN627_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HXD03348.1"
                     /translation="MQDQQQPQPLSGAPMWLAGFGLALANFVVILDTTITNVSVPHIA
                     GGLAVSPAQGTWTITSYAVAEAITVPLSGWLSARFGTLRTLVWALLGFGLFSVLCGLS
                     RTIEMLVVLRVLQGFCGGPMMPMTQTLMLRVFPPDKMGLANGLWGVTAISAPILGPLA
                     GGMIVDNLDWPWIFFINLPVVGVCYLTVTGLLGKFETPTRMERIDITGMVLLVLFAGS
                     LQLVLDLGRERDWFASGLIVTLAVVSAVAFAVFIAWELTDSHPVVDLRVLRHRSLAVG
                     LPVLAIGFGSMMAMLVLIPLWLQSVVGYTASSAGQLMAMQGVFAVLLSPVAAVLMQKV
                     DMRWPITAGLVITLATAIMRMGWTGDADFWTFATPQLIQGVGMPFFFIGVMTMALVGV
                     PQRDIAMAAGLLNFLRTVAGAAGTALSTSAWDNATRATRGELVGTLNGVDQMTAQMAG
                     GREIGLAVVERLVEEQAATIGSAHVFGLAMVAIAVTTALVWLMPRTVAQGTAGIPAAH
                     "
     gene            3656..4537
                     /locus_tag="VN627_08585"
     CDS             3656..4537
                     /locus_tag="VN627_08585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HXD03349.1"
                     /translation="MKFGLSMFGLGPRHYARVAQAAEAAGFDSVWLPDHVVFPETIPA
                     TYPYDASGVPGMDSSVGVFDPWVILAAVAQATTTILMGTAVYILPLRHPIIAGRALVT
                     LDHLSNGRVLLGAGVGWLEPEFEIMGEDFTKRGKRVDETIPLLRRMWTEPTIEHHGEF
                     FDMPPFKFNPKPRKGTIPILIGGDSPAALRRAGRLGDGWLEIGLQSLEELSGRLAKVK
                     AARSEAGLPEAPFDVTLIMRPKDMALTDELVRQLEDLGVGRIIVGQVAKATGGITVDT
                     ACEWAAEFGRETISRYA"
     gene            complement(4619..7405)
                     /locus_tag="VN627_08590"
     CDS             complement(4619..7405)
                     /locus_tag="VN627_08590"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HXD03350.1"
                     /translation="MLGPDDLSIPALEQRLQQLHPLTVQTPLFNPVFQLGHELSRRLE
                     DGSLTLAALDSLVAELECESLQARSRRLVRMLGPVAPQANAAAIAGLSARADFAEFAT
                     RWQRPFVHVVFTAHPTFLLSRAQSQAVVAAASADAVDAASVCVAPAARDAISLDYEHD
                     EAMKALIHAQAARDRIVGQVLDEARSRWPRRWREVRPAPFRFATWVGYDMDGRTDIGW
                     HTSIRYRLAEKAERLSSYAAALAQPAPDLAARLARASARAAEMAQLFAPDLADPAALS
                     AAANTLTADHPDKLTSLAAIISELEAEARHADDDVARALLVTAAAMRADGLGMGWIHF
                     RVNASQLQNAIRRRIDPDGRLDLASQAALVRMRELLAEARPLRSNFAALAIENSTAIR
                     QFLAMAQILAHVDADSPIRMLVAECEQPTTVLAALYFARLFGIADKVDVSPLFETESA
                     LEHGGRFLDALLAEEAYRDYARTRGRVAIQTGFSDAGRFVGQIPAALAIERLQGRLAE
                     AMAANGLGDVAALVFDTGGESMGRGCHPSSFTDRVDWQLSPWARRRFARAGIRLEPEV
                     SFQGGDGYLAFGSPELALATLTRFAENAPAEADPAAPTDPFYRRTDISLDFYRAIRRF
                     QHELLVSPTYSRAVTAFGLGLLNDTGSRKSRRQSDLAADRQMNLRQIRAIPHNAVLQQ
                     LGYPVNVIAGFGTAADGNAEEVAALLADSVRGRQTVRLVRAANALASIKTVAAFGELF
                     NSAYWASRPYRGDERQIEPACLALAEYLIGDDRAGVFRRLASQLRVDALKLHRLLALI
                     PDEAPVASREQDRRMMGVLQAIRLALLQHMFLRAVQVPTFSRANDISREDVLEMVFTL
                     RIDEALAQLRRAFPTSFPEPSDFSVTEPSDYPDDMASSYASIQQAFVDPIERAHGLIL
                     RITTAIANEFGAHG"
     gene            complement(7417..>7847)
                     /gene="eda"
                     /locus_tag="VN627_08595"
     CDS             complement(7417..>7847)
                     /gene="eda"
                     /locus_tag="VN627_08595"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HXD03351.1"
                     /translation="VVGAGTVLNPAQLDAALSAGAEFIVSPGLTPGLGRAAIDSGVPF
                     LPGTANAADIMLGLDLGLTRFKFFPAMASGGLPALKALAAPFHMARFCPTGGISAATA
                     KDWLAEPAVLCVGGSWLVPPGPPDPGAIEAAARAAANLAG"
BASE COUNT         1161 a         2379 c         3111 g         1196 t
ORIGIN      
        1 tcggcgtctt tctgggtgat cgggaagtag ccggtgggag tgggcttccg agaggtgccg
       61 aggaggacgg cggtggcggc gatctcgtag ccgccgcgga acaccgagag gacgcgggca
      121 tcgagatcga cggtgatgac cagcggcccg gcgggaacgc cggcttcgtc ccagtgctat
      181 tcgccatagc ggatcgggcc ggcgatcggg agaatgcgct tgatgacgag gcgcggggcg
      241 gcgggtgacg ccggtgccgg tgccggtgcc ggggcgggtg ccggggcggc ggcgggtgcc
      301 gccatggggg cagcggccgg agctggcgcc tggctgatgc gggcaggatc gatctcgagc
      361 ggggcggggc cggcgacggg ctggctggcg gaggtagccc ggcgatccac atggccgaac
      421 agcagcccgc caccggcgag cgtgaggccg agcgcggtga tcgccagacc tgcccaaccc
      481 tgcttgcgaa tccgcatttc cgtttacccc ttgatggtcc ggttctggcg ggaatcggcg
      541 gtcggcgcca gccttgcgcc ggcggcgtcc cggcgcggga ctcaggcgcg gatgaatctt
      601 aacgaggcgg ccggcgcggg ctggacggcc gccacttcgg cgacgagccg ggcatagcgc
      661 gccaggatcc ggtcgcgcgc gaagcggtgg acgctgcgcc ggccttcggc ggcgagggcc
      721 gcacggtggg cagggttggc cagcaggcgc cggatggcat gggccatggc caccgcatcg
      781 ccgggtggca ccagcgtgcc gttgacgcca tcggcaacga gatcggccga gccgtagtcg
      841 caatcggcca gcaggagcgg cgcgccggcg gccatggctt cggcggcggc attggggaaa
      901 ccttcccagc gcgagggcag gacgaacagg tccgccgcag ccatccaggc atggggattg
      961 gcggtgaacc cgacgaagtg gacccgctcg gcgataccga ggccagcggc ctgggcgcac
     1021 agcgcggcgt ggcggctgcc ctgtccgagg atgaccaatt catgggagct cgccatgcct
     1081 gaccgggcga aggcttcgag caggaggtcg tggccctttt gcggttcgag ccggcccacg
     1141 gcgaggatat aggggcgggc cggcaagtcg ggcgcgggga tgcttgccgc ctgttcgatc
     1201 gcggcgatgt cgacggggtt gtggatgagg tggacgcggc ccggatcgag cccgagcagg
     1261 cccagcaggt cgccggccat gccgcgcgca ttggcaagga tcgcgtctgc cgtgcggtag
     1321 gccatgccgg tgagcgcgag ggaagcggcc tggacgaggc ggcccgggct ctcctgccgg
     1381 gcggtggcga ggatgttgtt gccttcgcgg atgatccagc gaggccgggc cgaggcgggc
     1441 atgccggcca gcgcgcgcca ggtgaggatg gcggtgccct tgaggaagct catcaccacg
     1501 tcgggccgga cctgcgcgat gatgcggcgg atggcgccgg gggccagggc cgcgccggtg
     1561 gcgagggtgg cggggttgag cagttcgcgg ttcggccggt cgaagccgaa ccgggcggcc
     1621 tcgggggcga cgtggacccg ggcgggatcg agctggtcgc gccaggcctg gccgtcgtgc
     1681 agcaggccga gatggacatc gagcgggggc gctccggcga gcggggcgag ggcgtggcca
     1741 agcgcaggaa ggccgttcat caggtggacg gcgacccgct cggccccgcc gccagtccag
     1801 ttgccgagaa ggagcaggag ccgcagcggg ggtcccgtgc gctctgcggt gatccgtgcg
     1861 tgatgcatga caggtaaatc tcacaagcgc ggcggcggtt ccgtgcaggc acggcggtga
     1921 ttgcgcttcg tcttcccggg cgggccctgc tatggagggg ttacgggggt ttggggcggg
     1981 cgacgatgca ggatcaacag cagccgcagc cgttgagcgg cgctccgatg tggctggcgg
     2041 ggttcggcct tgcgctggcc aatttcgtgg tcattctcga cacgacgatc accaatgtct
     2101 cggtgccgca catcgccggc gggctggccg tttctcccgc tcagggcacc tggacaatta
     2161 cctcctatgc cgtggccgag gcgatcacgg tgccgctgtc gggctggctc tcggcccgct
     2221 tcgggaccct gcgcacgctg gtctgggcgc tgctcgggtt cggcctgttc tcggtgctgt
     2281 gcggattgtc gcggaccatc gaaatgctgg tcgtgctgcg ggtcctgcag ggcttctgtg
     2341 gcgggccgat gatgcccatg acccagaccc tgatgctgcg ggtattcccg ccggacaaga
     2401 tgggcctggc caatggactg tggggcgtga ccgccatttc ggcgccgatc ctcgggccat
     2461 tggccggagg catgatcgtc gacaacctcg actggccgtg gattttcttc atcaacctgc
     2521 cggtggtcgg ggtctgctac ctgacggtca ccgggctgct gggcaagttc gagaccccga
     2581 cgcgaatgga gcggatcgac attaccggca tggtcctgct ggtgctgttc gcgggttcgc
     2641 tccagctggt gctggatctc ggccgggagc gtgactggtt tgcctccggc ctgatcgtga
     2701 ccctcgccgt tgtctcggcc gtcgcctttg cggtgtttat cgcctgggag ctgactgact
     2761 cccacccggt ggtcgacctg cgcgtcctgc gccaccgcag cctggcggtg ggcctgccag
     2821 tgctggcgat cggcttcggg tcgatgatgg ccatgctggt cctgatcccg ctgtggctgc
     2881 aatcggtggt gggctatacc gccagcagcg ccgggcagct gatggcgatg cagggggtct
     2941 tcgcggtgct gctgtcgccg gtggcggcag tgctgatgca gaaggtcgac atgcgctggc
     3001 ccatcaccgc gggactggta atcacgctgg cgaccgcgat catgcgcatg ggctggacgg
     3061 gtgacgcgga tttctggaca tttgccacgc cccagctgat ccagggcgtg ggcatgccgt
     3121 tctttttcat tggcgtgatg accatggccc tggtgggggt tccccaacgg gacatcgcga
     3181 tggcggcggg attgctcaac tttctgcgca ccgttgcggg tgctgccggc acggcgctgt
     3241 ccacctcggc ctgggacaat gcgacccgcg ccacacgcgg cgagctggtg ggcacgctca
     3301 acggcgtgga ccagatgacc gcgcaaatgg ccggcgggcg ggagatcggg ctggcggtcg
     3361 tggaacggtt ggtcgaggag caggccgcca cgatcggttc ggcccatgtc tttggcttgg
     3421 cgatggtggc gattgccgtg accaccgcgc tggtgtggct catgccccgc accgtcgcgc
     3481 aagggacggc ggggataccg gccgcgcact gaaccccgcc gcgcgccggt gggggcgatc
     3541 catcagccat ctgcgcgctc gcgcccgcga ttgaccgtct ggagcgaggg ccatagcctt
     3601 tccgggacaa cgcccgggca ccagccacgg tgggcaggac cagcggagag agcggatgaa
     3661 attcgggcta tcgatgttcg gccttgggcc gcggcattat gcgagggtgg cgcaggcggc
     3721 ggaagcggcc ggcttcgatt cggtctggct gcccgaccat gtggttttcc ccgagacgat
     3781 cccggcgacc tatccctatg acgcttccgg cgttcccggc atggattcca gcgtgggcgt
     3841 gttcgatccc tgggtgatcc tggctgccgt ggcccaggcg acgacgacga tcctgatggg
     3901 cacggccgtc tatatcctgc cgctgcgcca cccgatcatc gccgggcggg cgctggtgac
     3961 gctggatcac ctgtccaacg ggcgcgtgct gctgggggca ggggtcggct ggctcgaacc
     4021 cgagttcgag atcatgggcg aggacttcac caagcgcggc aagcgggtgg acgagacgat
     4081 cccgctgctg cggcggatgt ggaccgagcc gacgatcgaa caccacggcg agttcttcga
     4141 catgccgccg ttcaagttca atccgaagcc gcgcaagggg acaatcccga tcctcatcgg
     4201 cggcgacagc cccgcggcgc tgcgccgggc cggccgactc ggcgatggct ggctggagat
     4261 cgggctgcag tcgctcgagg aactttccgg ccggcttgcc aaggtgaagg cggcgcggag
     4321 cgaggcgggg ctgccggagg ctccgttcga cgtgacgctg atcatgcgtc ccaaggacat
     4381 ggccctgacc gatgagctgg ttcgccagct ggaggacctt ggcgtcggcc ggatcatcgt
     4441 tggccaggta gccaaggcta ccggcgggat cacggtcgac accgcctgcg aatgggcggc
     4501 ggagttcggg cgggagacga tttcgcggta cgcctgaggg ggcgttgaac cccctccccc
     4561 agcccctccc cgacggggag gggagagcgt aggccgaacc ctttgggggt ggttgcgctc
     4621 agccgtgggc gccgaattcg ttggcgatgg cagtggtgat ccgcaggatc aggccgtggg
     4681 cccgctcgat cgggtcgacg aaggcctgct ggatcgaagc gtagctggag gccatgtcgt
     4741 cggggtagtc gctcggctcg gtgaccgaga agtccgacgg ttcgggaaag ctcgtcggga
     4801 aggcccggcg cagctgggcc agggcttcat cgatgcgcag ggtgaagacc atctcgagca
     4861 cgtcttcgcg gctgatgtcg ttggcgcggc tgaaggtcgg gacctggacc gcgcgcagga
     4921 acatgtgctg gagcagggcc aggcggatcg cctggaggac gcccatcatc cgccggtcct
     4981 gttcgcgcga ggcgaccggt gcctcgtcgg gaatcagggc gagcaggcga tgaagcttca
     5041 gcgcatcgac ccgcagctgg gaggcgaggc ggcggaacac gccggcgcga tcgtcgccga
     5101 tcaggtattc ggcaagggcg aggcaggcgg gctcgatctg gcgctcgtcg ccccggtagg
     5161 gccggctcgc ccagtaggcc gaattgaaca gctcgccgaa ggctgccacg gtcttgatcg
     5221 aggcaagggc attggcggcg cgcacgagcc gcacggtctg ccgcccgcgc acgctgtcgg
     5281 ccagaagggc ggcgacttcc tcggcattgc catcagccgc cgtgccgaag ccggcgatga
     5341 cgttgaccgg atagccgagc tgctggagca cggcgttgtg cgggatggcg cggatctggc
     5401 gcaggttcat ctggcggtcg gccgcgagat ccgactggcg gcgcgacttg cggctgccgg
     5461 tatcattgag caggccaagg ccgaaggcag tgaccgcgcg ggaataggtc gggctgacca
     5521 gcagttcgtg ctggaagcgg cggatcgcgc ggtagaaatc gaggctgatg tcggtgcggc
     5581 ggtagaacgg atcggtgggc gcggcgggat cggcttcggc cggggcgttc tccgcgaagc
     5641 gagtgagggt ggcaagcgcc agctcgggcg agccgaaggc gagataacca tcgccgccct
     5701 ggaaggagac ttccggttca agccggatgc cggcgcgggc gaagcggcgg cgggcccagg
     5761 ggctgagctg ccaatcgacc cggtcggtga aggaggaggg atggcagccg cggcccatcg
     5821 attccccgcc ggtgtcgaag acgagggcag cgacatcgcc aaggccattg gcggccatgg
     5881 cttcggccag gcggccctgg aggcgctcga tcgcgagggc ggcggggatc tggccgacga
     5941 agcggccagc gtcggaaaag ccggtctgga tggcgacccg gccgcgagtg cgggcatagt
     6001 cgcggtaggc ttcctcggcc agcagggcat cgaggaagcg gccgccgtgt tcgagggcgc
     6061 tttccgtttc gaacaggggc gagacatcga ccttgtcagc aatgccgaac aggcgggcga
     6121 aatagagcgc ggccaggacc gtggtcggct gctcgcattc ggcgaccagc atgcggatcg
     6181 ggctgtcggc atccacgtgg gcgaggatct gcgccatggc gagaaactgg cggatcgccg
     6241 tggaattctc gatcgccagc gcggcgaaat tggagcgcag gggcctggct tctgccaaaa
     6301 gctcgcgcat gcgcacgagg gcggcctggc tggcaaggtc gagccggcca tcggggtcga
     6361 tccggcggcg gatggcattc tgcagctgcg aggcgtttac ccggaaatgg atccagccca
     6421 tgccgaggcc atcggcacgc atggccgcgg cggtgaccag cagggcgcgg gcgacatcgt
     6481 cgtcggcatg gcgggcctcg gcttcgagct cgctgatgat cgccgcgagg ctggtgagct
     6541 tgtcggggtg atcggcggtg agggtgttgg cggcggcgga gagcgcggcg ggatcggcaa
     6601 gatcgggggc gaacagctgg gccatctcgg cggcgcgggc cgaggcgcgg gcgaggcggg
     6661 cggccagatc cggcgcgggc tgggcgagcg cagcggcata ggacgagagc cgctcggcct
     6721 tttcggcgag gcgatagcgg atcgaggtgt gccagccgat atcggtgcgc ccgtccatgt
     6781 cgtagccgac ccaggtggcg aagcggaacg gggccgggcg aacttcgcgc cagcgccggg
     6841 gccagcggct gcgcgcctcg tcgaggacct ggccgacgat ccggtcacgc gcggcctggg
     6901 cgtggatcag cgccttcatc gcctcgtcgt gctcatagtc gaggctgatg gcatcgcgcg
     6961 ccgccggggc gacgcagacc gaggcggcat cgacggcatc ggccgaggcg gcggcgacca
     7021 cggcctggct ctgggcgcgg gagagcagga aggtggggtg ggcggtgaag accacgtgga
     7081 cgaacggacg ctgccagcga gtggcgaatt cggcgaagtc ggcccgcgcc gagaggccgg
     7141 cgatggccgc ggcgttggcc tggggcgcga ccgggccgag catccgcacc agccgtcggc
     7201 tgcgcgcctg gagactttcg cattcgagct cggcaaccag gctgtcgagc gcggcgaggg
     7261 tcaggctgcc gtcttcgagg cggcgcgaca gctcgtggcc gagctggaac accggattga
     7321 acagcggggt ctggaccgta agcggatgga gctgctggag gcgctgctcg agcgcgggga
     7381 tggaaaggtc gtccggtccg agcatgggcg ttggtcctag ccggcgagat tggcggcggc
     7441 gcgagcggcg gcttcgatcg cacccggatc gggcgggccc ggcgggacga gccacgaacc
     7501 tcccacacat aggacggcag gttcggcaag ccaatccttc gcagttgcag cagagatgcc
     7561 gccggtcggg cagaagcggg ccatgtggaa cggggctgcc agggccttga gtgccggcag
     7621 gccgcccgac gccatggccg ggaagaactt gaagcgggtc agcccgaggt cgagaccgag
     7681 catgatgtcg gccgcgttgg ccgtgccggg gaggaagggg acgccgctgt cgatcgcggc
     7741 gcggccgagc ccgggcgtga ggccggggga gacgatgaat tccgcgccgg cggaaagcgc
     7801 ggcatcgagc tgggcggggt tgagtacggt gccggctccg acgacgg
//
LOCUS       DATNBV010000362        12429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4042748,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000362 DATNBV010000000
VERSION     DATNBV010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 12429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12429
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4042748"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VN627_08600"
     CDS             complement(<1..314)
                     /locus_tag="VN627_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HXD03352.1"
                     /translation="MTSNLYPHLLSPGRIGSMELANRIVVTAMGVSLSEDDGTVGERL
                     IAYHEDQARGGAGLIVSGVAGVAWPVGAVSMQQTAISEDRFIPGLKALTDRVHACGAK
                     IAA"
     gene            740..1135
                     /locus_tag="VN627_08605"
     CDS             740..1135
                     /locus_tag="VN627_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXD03353.1"
                     /translation="MTAVSGFAKLICRDEEAMANYYMAVYGYGLVQRVAGESDGEPFR
                     EVILAPGGDWSRGSLVMFNFTDRAAPRDQQVILGFVVEDLDAVAKAIVANGGKLVGPL
                     RDEPEHGVRLVFATDPEGALSENVQMLAQ"
     gene            1230..2084
                     /locus_tag="VN627_08610"
     CDS             1230..2084
                     /locus_tag="VN627_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03354.1"
                     /translation="MARDKDVFEGGVAVITGAGSGIGAGLARRAGALGMTVVVTDINL
                     AAAEQTVAEIKAAGGKAEAEKVDVSIPEELDRLAATVFGRHGSVRLLINNAGIETVGN
                     TWEIPTARWEATLNINVHGVIHGVRAFVPHIIASGQEAWIGNLSSVGAFGQMPGQTAY
                     TVTKHAVQSFSECLYLEMELKGLPIHVSAIVPGMLRTNIFNAEGGAGEPASAANHRRA
                     MFEMMRDYGMDLDEGCRLFLEQMAENKFWAHSQPEMSEQIIGGRIKFLQEQTPPIMPD
                     LARQIAVD"
     gene            2194..3159
                     /locus_tag="VN627_08615"
     CDS             2194..3159
                     /locus_tag="VN627_08615"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HXD03355.1"
                     /translation="MFPIIGALIPVFALIALGIVLGRLGLLGAGADEVLSRFVGLVTL
                     PVLTFLAISGLKAEQLAQPVMATVVIAGAWLTFALQLSVERLCGRPGPEANVLALSAA
                     FGNSGFVGLPICLAILGKSALGPAALVMALTTGFVFAPFILLQELTGSLGHSRSESIR
                     TVSRALVGNPLILSCIAGIAAALGGVSLPAPVQVLLSSIAAATAPCALVAIGMFVARP
                     VEQASDPGIWRGWGAKLALSPLMAAALLAVLPPLDRVWTATALIMAAMPTASSVFVIV
                     AGDTARRISASVIVRTTVGSVVTIPIVLAVLAYAGVLDLGPAPLH"
     gene            complement(3167..4072)
                     /locus_tag="VN627_08620"
     CDS             complement(3167..4072)
                     /locus_tag="VN627_08620"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03356.1"
                     /translation="MTMTKVGYIGLGAMGLPLAKHLTRKGYDVIAYDIDPARADAIVE
                     LGGRKAQSVAEAARDVEVVITCLPATPHVEAVVLGADGALAQMKPGTLLLEMSTIDAN
                     GTDRVARACAEKGVAFVDSPIGRLVLHAERGESLFMVGATDEDFARVEPLLKAMGKDI
                     MRCGGRGMGSRMKLVNNFLLLTIAQVSAEAVALGTRLGLDIDTILEVTGSTTAQNGQL
                     HTLMVNKVLKGDTQPGFTIDLAFKDMTLAMTAAAETRMGLPVGAAAHAVYGGARAVPE
                     YAAKDYSALLEWACRNAGIETPRTA"
     gene            complement(4088..5524)
                     /locus_tag="VN627_08625"
     CDS             complement(4088..5524)
                     /locus_tag="VN627_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXD03357.1"
                     /translation="MKGANYIDGRWVDGAVPFETVNPSDLDETVGHYTKVGEAEVREA
                     MAAARRAQPAWGAMNMQARADILHKAADLIKARAQEIGLLLSREEGKTVPEGVGEALR
                     AAQCFQYSAGDVVRAQGEWYNSMRDGFNVLVTREPVGVVAAITPWNFPIALPSWKVAG
                     ALAHGNSVVLKPSSFVPGCAVLLAQILEGVGVPAGVFNLVMGDGRATGNVMIDEADAL
                     TFTGGTATGRSVLTRAAETMTKCQLELGGKNALVVLDDADLDLAVDIASNGAWIQTGQ
                     RCTGTERLIVTRGIHDAFVERMAKVAAAYRVGHAQDADTQIGPVANAPQFNDNLRFVE
                     DARAEGAKVAAGGGAVEARTRGLFMAPTLLVGTKPEWRCNQHESFGPIASVVMVEDLD
                     EAIHAANACEYKLSSGIATSSLKNAERFRKASQAGMVMVNAPTAGLEYHVPLGGRAPS
                     GYGPRETGASTAEFFTESKTSYINHGAI"
     gene            complement(5524..6777)
                     /locus_tag="VN627_08630"
     CDS             complement(5524..6777)
                     /locus_tag="VN627_08630"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXD03358.1"
                     /translation="MSNEVHPGAGAAGPLAGVKVLDLSRVLAGPWCSQILGDLGADVI
                     KVEQPGQGDDTRRWGPPFLDDGSRDSAYYLCANRNKQSVAINMADPQGAALIRKLATE
                     ADIVVENFRVGGLAKYGLDYETLRALKPDLIYLSITGFGQTGPLRDNGGYDFLVQGMS
                     GLMSVTGLPDGEPGGGPMKVGIPISDLTTGLYAAISVLAALNHRHATGEGQRIDLALL
                     DTQVALLANQASNWMNGGMRPRRLGNQHPNTVPYQDFACSDGDILVALGNDRQFRAFS
                     ELIGLPGLPDDERFSSSAGRSVNRDALLAQMRPAIARWKSQDLIAAMEAAKLPGGKVN
                     EIPEVLEHPQIKARGLVHDMERSDGTPVKVLGFPAQLSASPATYRHAPPRSGEDTFGV
                     LMERLGLDRHELERLAQAGVIAEKL"
     gene            6939..7814
                     /locus_tag="VN627_08635"
     CDS             6939..7814
                     /locus_tag="VN627_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXD03359.1"
                     /translation="MDLRQLRYFSVLAETLNFHRAAERLNISQPPLTVAIRKLEDDLG
                     VALFDRDPRGARLTAAGQAALAPAREALAAADQMRQAVRQGAAGLSGRLAVGFIGSAI
                     GELLPRIVSPFRQAYPQVELSLEELNSVDIVRGIAARRLDVGLVRLPVMDSAPVRIDV
                     IERDVLVAAMPRDDLLARRKVVPLAALSDRSFILYSPVSVLHATIRLECHRAGFTPLV
                     TQEAVQVQTILSLVEAGLGVALIPARSARFAGAGVHIAALEQPIAITMGIARAQDASA
                     AALNFAATALGHCGL"
     gene            complement(7851..10967)
                     /locus_tag="VN627_08640"
     CDS             complement(7851..10967)
                     /locus_tag="VN627_08640"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HXD03360.1"
                     /translation="MKVNRREAIGIAVAGSSAVAAPLRAFAAPPTAGIEALRVCGLDN
                     PLALGDLTPRFSWKLGGGARSPQAAYRLCVARSEADLIGRRNLVWDSGRVVSASGVDQ
                     PYGGPRLPSRSKVWWQVETWTKDGKSLRSAPAMWETGLAPADWQGEWIASETLVAQAD
                     RTAGLHWITGPDRQNIGQTRAFRWTFDHPGGPAEFCATLHELTGVWINGEPAPEPRDG
                     PARHTEMAVWRVNLQKGRNVIAIAGRRVGSFGVPTCVLAALLRHGPELRERMTSASPG
                     WKTAIAPPQGWNAPGFDDAAWAAAVPVTGGSLPVGEPWPLTPASRLRHVFTVDRPIRA
                     ARLHATAAGIYQPWLNGAAIGDRKLAPEFTDPSKRLLYQTYDVTAHVKPGANVLGMVV
                     GDGWYGSRFSTSARFAFGPAPCRMLCQLELEHDDGSRTVIGSGKDWQIADSGVMGHSI
                     YDGEIFDARVERADWAMSGSAEQGWRAASVVPAPPAPVDPQRCPPIRVRETLQPKDVR
                     RLGPGRYVLDFGQNFAGFERLTLAAPAGTRIEMHFAEVLKADGSVDQANLRSAKARDV
                     YIAAGSGREVYEPRFTYHGYRYVEITGVPDDRSAWSLEGLVAHQDLPFTAELSVGDPV
                     IEQFWRNSVWSQKSNFYGLPTDCPQRDERLGWMGDAEVFWPAAAWNMDVTAYTARVME
                     DVRSSQSAQGAFPDCIPPFVPTMKLSSPGWADAGVILPYTSWMQNGDTGVVRANWSAM
                     ETYMAWIEKANADHVWRKQRGADYGDWLSVDAPTSNPGAPTTPKDLLATALWANDAAM
                     MAQMAEALGNAAAAARYRDLFQTIRGAFNATFVSADGKVGNGSQTGLVVAIRFGLLSP
                     EQTRAAGAALAADIARRGNHLSTGFIGTPHILDALAMSGQEAMAITLLTQRTFPSWGY
                     MVDKGATTMWERWNSDQGDVGMNSRNHYAFGAIGDFLFRRIAGIAPATPGFGKVRIAP
                     IMSPALGKGGATYQSVRGPIRTAWTAGGGQFRLDVDLPAGVDGEVILPGGRAARAVAG
                     PNRFSGKL"
     gene            complement(10999..>12429)
                     /locus_tag="VN627_08645"
     CDS             complement(10999..>12429)
                     /locus_tag="VN627_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HXD03361.1"
                     /translation="WGFKGFVMSDWGAVHSTEALLAGLDQQSGELIDKKRWFSTELKP
                     RLADGTIPASAVDTAVKRILWSMYANRADITPRGQTPIDYAKNGEVALEAAREGTVLL
                     RNEGGLLPLARTVRSVVVIGGKADMGVPSGGGSSQVVPVGGFRATERLLGGPAASFAR
                     KAWGGSVPLAALRDALPGVNVTFVDGSQPAEAAAAAKAADMAIVFATKFSTEAEDQRD
                     LSLDGAQDALIDQVASGNPRTVVVLETGNAVAMPWLGKVPAVLSAWYSGQRGGEAIAE
                     IITGKVNPSGRLPVTFPVSVDQLPNPKFPGADLPPPSKEDKATYGLNTNSAPFSITYP
                     EGSDVGYRWFDRKGEKPLFAFGHGLSYTSFGYSGLKVTGGRTLSASFTVTNTGQRPGA
                     DVPQVYVTLAGKARRLVGWDKLVLNPGESREISLTADPRLLADFNARARRWEVRPGTA
                     KVELARSAAEPVLSAQVVLKRQTIKP"
BASE COUNT         1922 a         4293 c         4121 g         2093 t
ORIGIN      
        1 gccgcgatct tcgccccgca ggcatggacc cggtcggtca gcgccttcag cccggggatg
       61 aagcggtcct ccgaaatggc ggtctgctgc atcgaaaccg ccccgaccgg ccaggcgacg
      121 cccgccacgc cggatacgat cagcccggcg ccgccccttg cctggtcttc gtgataggca
      181 atcaatcgct ctccgacggt cccgtcgtct tccgaaagac tgacccccat ggccgtcacg
      241 actatgcggt tcgccagttc catcgagccg atgcggcccg gggagagcag gtgcgggtaa
      301 agattggagg tcacgggcgc ggcctttctc aggtgttcat ggccgacctg cgccggcgca
      361 agggcaaggt gcgcttgcgg cacccgccgg attcaataat cacggggccg actgacgatc
      421 ggacggtcag gagagcaggg ctcggattgt gccgtcctgc gccgccaggg caagcaaggt
      481 ggcacagccc gtgaaggccc cgttcctcgg ccggaatgcc cgctgatggc atcctgtgca
      541 tggctgctaa ccgcccgcat tttccctctg attgcgcctt tcggcttgtc attgcgtaat
      601 caatgcgcga ttacgttacg ataatcaagg gttggacaag cgtaatcgag ccagcccgca
      661 cgaggtgcac tgggccaatc cattgccctg cgaacggtcg ccccgccggg cgccgcaaca
      721 agaacgggag acttccggga tgactgcagt ttcaggcttc gccaagctca tctgccgcga
      781 cgaggaggcg atggccaact actacatggc tgtttacggc tatgggctcg tccagcgggt
      841 tgctggcgaa agcgacggtg aacctttccg cgaagtgatc ctggcgccgg gcggcgattg
      901 gtcgcgcggt tcgctcgtca tgttcaactt caccgaccgc gccgccccgc gcgaccagca
      961 ggtcatcctc ggcttcgtcg tcgaggatct ggacgcggta gccaaggcga tcgtcgccaa
     1021 tggcggcaag ctggtcggcc cgctcaggga tgagccggaa cacggcgtgc gccttgtgtt
     1081 cgcgaccgac ccggaaggcg ccctgagcga gaacgtgcag atgcttgcgc agtaagcagg
     1141 cgacggccag aggcgattcg ccggggccga taaactgatg ggcgtggccg tgaagctggc
     1201 ctcgcgcaga actacgggag agcgggtaaa tggcacgcga caaggacgtt ttcgagggcg
     1261 gagtggccgt gatcaccggg gcgggctcag gcatcggggc gggcctggcc cgccgggccg
     1321 gcgcgctggg tatgaccgtc gtcgtaaccg acatcaacct ggccgcggcc gagcagacgg
     1381 tggccgagat caaggcagcc ggcggcaagg cggaagccga gaaggtcgat gtctcgatcc
     1441 ccgaagagct ggatcgtctg gccgccacgg tgttcggccg tcacggctcc gtccggctgc
     1501 tgatcaacaa cgccggcatc gaaaccgtcg gcaatacctg ggaaattccg acggcgcggt
     1561 gggaagccac gctgaacatc aacgtccacg gcgtcatcca tggcgtccgc gccttcgttc
     1621 cgcacatcat cgccagcggc caggaagcgt ggatcggcaa tctgtcgtcg gtcggagcct
     1681 tcgggcagat gccggggcag acggcctaca cggtcaccaa gcatgcggtt cagtcgttca
     1741 gcgaatgtct ctatctggag atggagctga aaggcctgcc gatccacgtc tccgcgatcg
     1801 tgccgggcat gctgcgcacc aacatcttca acgccgaggg aggcgcgggc gagccggcca
     1861 gcgcggccaa ccaccgcagg gccatgttcg agatgatgcg cgactacggc atggacctcg
     1921 acgagggctg ccgcctgttc ctcgagcaga tggccgagaa caagttctgg gctcacagcc
     1981 agcctgagat gagcgagcag atcatcggcg gccgcatcaa gttccttcag gaacagaccc
     2041 cgccgatcat gcctgacctt gcccgccaga tcgcggtcga ctgaacccat gcagccagaa
     2101 caggcgctcg gccggcttgc gggcacggtc gccatcgtga ccggcgggtc gatctgtcac
     2161 gatggtgcca tcggcctcag gtactgagcc tgcgtgttcc cgattatcgg cgcattgatc
     2221 ccggtcttcg cactgatcgc gctgggcatc gtccttggcc gcctcggctt gctgggggca
     2281 ggcgccgacg aggtgctgag ccgcttcgtt ggcctggtga ccttgccggt tctcaccttt
     2341 ctggcgatct ccggcctcaa ggctgaacag cttgcccaac cggtgatggc tacggtcgtc
     2401 atcgccggcg cctggctgac tttcgcgctg cagttgtcag tcgagcgcct gtgcggcagg
     2461 cccgggccgg aggcgaacgt cctcgccctg tcggcagcct tcggcaacag cggctttgtc
     2521 ggcctgccga tctgccttgc catactcggc aagtccgccc ttgggccggc agccctggta
     2581 atggcgctca ccaccgggtt cgttttcgcg cccttcatcc tgttgcagga attgacggga
     2641 tcgcttggcc acagcaggtc cgagagcatc cggacggttt cccgcgcgct tgtcggcaat
     2701 cccctgatcc tgtcgtgcat cgccggcatt gcggcggcgc tcggcggcgt gtcgctgccg
     2761 gccccggtgc aagtcctgct gtcgtcgatc gcggcggcca ccgcgccttg cgcgctggtg
     2821 gctatcggca tgttcgtggc aaggccggtg gagcaggcgt cggaccctgg catctggcgc
     2881 gggtggggcg ccaaactggc gctcagcccg ctcatggccg cagcgcttct ggccgtgctg
     2941 ccgccgctgg atcgggtctg gacggccacc gcactgatca tggccgcgat gccgacggca
     3001 tcctcggtgt tcgttatcgt cgcgggcgat acggcgcgcc ggatcagcgc cagcgtcatc
     3061 gtgcggacca cggtcgggtc cgtcgtcacc atcccgatcg tgttggccgt gctagcctat
     3121 gcgggggtgc tggacctcgg cccggcaccc ttgcattgag cgcagatcac gccgtccgcg
     3181 gcgtttcgat ccccgcattg cggcaggccc attccagcag cgccgaatag tccttggcgg
     3241 catattccgg cacggcccgc gccccgccat agaccgcgtg ggcggcagcg ccgaccggca
     3301 ggcccatccg ggtctcggcc gccgcggtca tggccagggt catgtccttg aacgccaggt
     3361 cgatggtgaa cccgggctgg gtatcgccct tcagcacctt gttgaccatc agcgtgtgca
     3421 actgcccatt ctgcgcggtg gtgcttccgg tcacttcgag aatggtgtcg atgtcgagcc
     3481 cgagccgcgt gcccagcgcc acggcttcgg cgctgacctg ggcaatggtg agcagcagga
     3541 agttgttcac cagcttcatg cggctgccca tgccccgccc gccgcagcgc atgatgtcct
     3601 tgcccatggc cttgagcagc ggctccaccc gcgcgaagtc ctcgtcggtc gcgcccacca
     3661 tgaacagcga ttccccgcgc tcggcgtgga gcacgagccg gccgatcggg ctgtcgacga
     3721 aggcgacgcc cttctcggcg caggcgcggg ccacccggtc ggtgccgttg gcgtcgatgg
     3781 tcgacatctc gagcagcagc gtgcccggct tcatctgcgc gagcgcgccg tcggccccga
     3841 gcaccacggc ctcgacatgg ggcgtggccg gcaggcaggt gatcaccacc tcgacgtcac
     3901 gcgcggcttc cgccacggac tgcgccttgc ggccgcccag ctcgacgatc gcgtcggcgc
     3961 gggccgggtc gatatcatag gcgatgacgt catagccctt gcgggtcagg tgcttcgcca
     4021 gcggcaggcc catggcgccc aggccgatat atcccacttt ggtcatagtc actctccgcc
     4081 caggcgatca gatcgccccg tgattgatat agctggtctt gctctcggtg aagaactccg
     4141 ccgtagaggc gccggtttcg cgcggaccgt agccggaggg cgcccggccg ccgagcggca
     4201 cgtggtattc gaggcccgcc gtgggcgcgt tgaccatgac catgccggcc tgcgatgcct
     4261 tgcgaaaccg ctccgcgttt ttcaggctgc tcgtggcgat cccggaggag agcttgtatt
     4321 cgcaggcgtt ggccgcgtgg atcgcctcgt cgaggtcttc gaccatgaca accgaggcga
     4381 tcggtccgaa cgattcgtgc tggttgcagc gccattccgg cttcgtgccg accagcagcg
     4441 tcggcgccat gaacaggccg cgcgtgcggg cttccaccgc accgccgcct gccgccacct
     4501 tggcgccctc ggccctggca tcctcgacga accggaggtt gtcgttgaac tggggcgcgt
     4561 tcgccaccgg accgatctgg gtatcggcat cctgcgcatg gccgacgcgg tacgcggcgg
     4621 caaccttggc catgcgctcg acgaaggcat catgaatgcc ccgcgtcacg atcaggcgct
     4681 cggtcccggt gcagcgctgc ccggtctgga tccacgcccc gttgctggcg atgtccaccg
     4741 caagatcaag gtccgcatcg tcgagtacga ccagtgcgtt cttgccgccc agctccagct
     4801 ggcacttggt catcgtctcg gccgcgcggg tcagcaccga ccgcccggtg gccgtaccac
     4861 cggtaaaggt cagcgcgtcg gcctcgtcga tcatcacgtt gccggtggcg cggccgtcgc
     4921 ccatcaccag gttgaacacc ccggccggaa ccccgactcc ctcgaggatc tgtgcgagca
     4981 ggacggcgca gccgggcacg aagctgctgg gcttcaacac taccgaattg ccatgggcca
     5041 gcgcgccggc aactttccac gagggcaggg caatggggaa gttccacggc gtgatcgccg
     5101 ccaccacgcc caccggttcg cgcgtgacca gcacgttgaa cccgtcgcgc atcgaattgt
     5161 accactcgcc ctgcgcgcgc accacgtcgc ccgccgagta ctggaagcac tgggccgcgc
     5221 gcagcgcttc gcccacgccc tcaggcacgg tcttgccctc ttcgcggctg agcagcaggc
     5281 cgatctcctg cgcgcgcgcc ttgatgagat ccgcggcctt gtgcaggata tcggcgcgcg
     5341 cctgcatgtt catggcgccc cacgccggct gtgcgcgccg cgccgcggcc atcgcctcgc
     5401 gcacctcggc ctcgccgacc ttggtgtagt ggccaaccgt ttcatccaga tcggatgggt
     5461 tgaccgtctc gaatggcacg gccccgtcga cccagcgacc atcgatatag ttagcgccct
     5521 tcatcacagc ttctccgcaa tcacgccggc ctgggccagt cgttccagtt catgacgatc
     5581 aaggcccagc cgctccatca aaaccccgaa cgtatcctcg cccgaacgcg gcggcgcatg
     5641 gcgataggtg gccggggagg ccgagagctg ggccgggaaa ccgaggacct tgaccggcgt
     5701 accgtcggac cgctccatgt catggaccag cccgcgcgcc ttgatctgcg ggtgctcaag
     5761 cacctcggga atttcgttga ccttgccgcc gggcagcttg gctgcctcca tcgcggcgat
     5821 caggtcctgc gatttccacc gggcgattgc cgggcgcatc tgcgccagca gggcatcgcg
     5881 gttgacgctg cggcccgcgc tgctggaaaa gcgttcgtcg tccggcaggc cgggcagccc
     5941 gatcagttcc gaaaaggcgc ggaactggcg gtcgttgccc agcgcgacga ggatgtcgcc
     6001 gtcggagcag gcgaaatcct gatagggaac ggtgttgggg tgctggttgc ccaggcggcg
     6061 cgggcgcatt ccgccgttca tccagttcga agcctggttg gccagcagcg ccacctgtgt
     6121 gtcgagcagg gcaagatcga tccgctggcc ttcgccggtg gcatggcggt gattgagcgc
     6181 cgcgaggaca gaaatggccg catagagccc cgtagtcagg tcgctgatcg gaatgccgac
     6241 cttcatcggg ccgccgcccg gctcgccatc ggggagaccg gtgacgctca tcaggccgct
     6301 cattccctgg acaaggaaat catacccgcc gttgtcccgc agcggccccg tctgaccgaa
     6361 gcccgtgatg gaaagataga tcaggtccgg cttgagcgcg cgcagcgtct cgtagtcgag
     6421 cccgtacttg gcaaggccgc ccacgcgaaa gttctcgacc acgatatccg cctcggtcgc
     6481 aagcttgcgg atcagcgccg cgccctgcgg gtcggccatg ttgatggcca ctgattgctt
     6541 gttgcgattg gcgcacaggt aataggcgga atcccggctg ccgtcatcga ggaagggcgg
     6601 cccccaccgg cgggtgtcat cgccttgtcc cggctgttcc accttgatca cgtccgcgcc
     6661 cagatcgccc aggatctgcg aacaccatgg gcccgccagc acacgggaaa ggtcgagcac
     6721 cttcactccc gccagcggac cggccgcacc agcgccagga tgcacttcgt tgctcatctg
     6781 tcctcaaact ccggcgcagg tttttcgagg gggcgccgac tgcttcgcgg tgctcgagcg
     6841 ggccacctcc ggggaggtga tagtgggccg ggatgctagg ataatcgact gcataatggt
     6901 aatatcgatt tactgaatct tgatagggat cgtatatcgt ggacctccgt caactccgct
     6961 atttcagcgt cctcgccgag acgctcaact ttcatcgcgc ggcggagcgc ctcaacattt
     7021 cccagccgcc gctgaccgtc gcaatccgca agctggagga tgatctgggc gtggccctgt
     7081 tcgatcgcga tccgcgcggc gcccggctga ccgccgccgg ccaggccgcc cttgcgccgg
     7141 cccgggaggc gctggcggcg gctgaccaga tgcgccaggc ggtgcgccag ggggcggcgg
     7201 gcctcagcgg gcggctggcc gtcggcttca tcggctccgc catcggcgaa ctgcttcccc
     7261 gaattgtctc accgttccgg caggcctatc cgcaagtgga gctctccctc gaagagctga
     7321 acagcgtgga catcgtcagg gggatcgcgg cgcgaaggct ggacgtcggg ctggtgcggc
     7381 tcccggtgat ggactccgcg cccgttcgca tcgacgtgat cgagcgcgac gtgctggtgg
     7441 ccgccatgcc ccgcgacgat ttgcttgccc gccgaaaggt cgtcccgctc gcggcgctct
     7501 ccgaccgctc cttcatcctc tacagcccgg tcagcgtgtt gcacgcgacc atccggctcg
     7561 aatgtcaccg ggctggcttc accccgctcg tgacacagga agcggtgcag gtgcagacca
     7621 tcctcagcct ggtggaagcg ggcctcggcg ttgcgctgat cccggcgcga agcgcgcgct
     7681 tcgcgggggc aggcgtgcac atcgccgccc tggaacagcc gatcgccatc accatgggca
     7741 tcgcccgggc acaggacgcc tccgccgcgg cgctcaactt cgcggcgaca gccctcggcc
     7801 actgtggcct gtgatagcag gcgcgcgcgg cagcgccggt tcaccccgcg tcaaagcttg
     7861 ccggaaaagc ggttgggacc agcaacggca cgggccgccc gcccgcccgg caggatgacc
     7921 tcgccgtcca cgcccgccgg caggtcgacg tcgagccgga actgcccgcc gcctgccgtc
     7981 catgcagtgc ggatcgggcc gcgcacgctc tggtaggtgg ccccgccctt gcccagggct
     8041 ggcgacatga tcggggcgat gcggaccttg ccgaagccgg gcgtggccgg tgcgatcccg
     8101 gcgatgcggc ggaacaggaa gtcgccgatc gcgccgaagg catagtggtt gcggctgttc
     8161 atgccgacgt cgccctgatc gctgttccac cgctcccaca tggtcgtcgc gcccttgtcg
     8221 accatgtacc cccacgacgg gaaagtccgc tgggtcagca gggtgatcgc catggcctcc
     8281 tgccccgaca tcgccagggc atcgagaatg tggggcgtgc cgatgaaccc ggtcgagagg
     8341 tgattgccgc gacgggcaat atccgccgcc agggccgcgc ccgctgcccg ggtctgctcg
     8401 ggcgagagca ggccgaaccg gatcgccacg accagcccgg tctggctgcc gttgccgacc
     8461 ttgccgtccg cgctcacgaa ggtggcgttg aaggcgccgc ggatggtctg gaacaggtcg
     8521 cggtaacggg ccgccgcggc agcattgccc agggcctcgg ccatctgcgc catcatcgcc
     8581 gcgtcattgg cccacagcgc cgtggcgagc aggtccttcg gcgtggtcgg tgcgccgggg
     8641 ttgctcgtgg gggcatcgac cgacagccag tcgccataat cggcgccccg ctgcttgcgc
     8701 cagacatggt ccgcattcgc cttctcgatc caggccatgt aggtttccat ggccgaccag
     8761 ttggcccgga caacgccggt atcgccgttc tgcatccacg aggtataggg caggatcacc
     8821 ccggcatcgg cccagccggg cgatgaaagc ttcatcgtcg gcacgaacgg cgggatgcag
     8881 tcggggaagg caccctgcgc gctctgggaa gaacgcacat cctccatcac ccgcgcggtg
     8941 taggcggtaa catccatgtt ccacgccgct gccggccaga acacctccgc atcgcccatc
     9001 cagcccaggc gctcgtcgcg ctgcgggcaa tcggtcggca ggccgtagaa gtttgatttc
     9061 tggctccaca ccgagttgcg ccagaactgt tcgatgaccg gatcgccgac cgaaagctcg
     9121 gcggtaaacg ggagatcctg atgggcgaca agcccttcca ggctccacgc cgagcggtcg
     9181 tcgggcacgc cggtgatctc gacatagcga tagccatggt aagtgaagcg cggctcgtaa
     9241 acctcgcggc cgcttcccgc cgcgatgtag acgtcgcgcg ccttggccga acgcaggttg
     9301 gcctgatcga ccgagccatc cgccttgagc acttcggcaa agtgcatctc gatccgtgtg
     9361 cccgccggtg cggcgagcgt caggcgctcg aaaccagcaa agttctggcc gaagtccagc
     9421 acgtagcgtc cggggcccag ccgccgcacg tccttgggct gaagcgtctc gcgcacccgg
     9481 atcggggggc agcgctgcgg gtcgaccggc gccggcgggg cggggaccac gcttgccgcg
     9541 cgccagccct gctcggcgct gccgctcatc gcccagtcgg ctcgttcgac gcgcgcgtcg
     9601 aagatctcgc cgtcgtagat cgagtggccc atcaccccgg aatcggcgat ctgccaatcc
     9661 ttgccgctgc cgatcaccgt gcgcgagccg tcgtcgtgtt cgagctcgag ctggcacagc
     9721 atccggcacg gcgcgggtcc gaaggcaaac cgcgccgagg tgctgaagcg cgagccgtac
     9781 cagccgtcgc ccaccaccat gccaagcacg tttgcgccgg gtttcacgtg cgccgtcacg
     9841 tcataggtct ggtagagcag gcgcttggag ggatcggtga attcgggcgc gagcttgcgg
     9901 tcgccgatcg cggcgccgtt gagccagggc tggtagatgc ccgcggccgt cgcgtgcagg
     9961 cgcgccgcgc ggatcggccg gtcgaccgtg aacacgtgcc gcaggcggct ggcgggggtc
    10021 agcggccacg gctcgccgac cggcagcgaa ccgccggtga ccggcacggc agcggcccat
    10081 gccgcatcgt cgaagcctgg cgcgttccag ccttgcggcg gggcgattgc cgttttccag
    10141 ccggggctcg ccgaagtcat ccgttcgcgc agctcagggc catggcgcag cagcgcggcc
    10201 agcacgcagg tcggcacgcc aaagctgccc acgcggcgtc ccgcgatggc gatgacgttg
    10261 cggccctttt gcaggttcac gcgccacacg gccatctcgg tatggcgcgc ggggccgtcg
    10321 cgcggctccg gcgccggttc cccattgatc cacacgcccg tcagttcgtg cagcgtggcg
    10381 cagaattccg ccgggccgcc cgggtggtcg aaggtccagc gaaaggcgcg ggtctggccg
    10441 atgttctggc gatccggccc ggtgatccag tggagcccgg ctgtccggtc cgcctgcgcc
    10501 accagcgtct cgctggcgat ccattcgcct tgccagtcag cgggagcaag gccggtttcc
    10561 cacatggccg gcgcgctgcg cagggatttg ccgtccttcg tccaggtttc gacctgccac
    10621 cagaccttgc tgcgcgacgg cagcctcggg ccgccgtaag gctgatcgac accggatgcg
    10681 ctgacgaccc ggccgctgtc ccagaccagg ttgcggcgac cgatcaggtc agcttcgctc
    10741 cgcgccacgc agaggcgata ggctgcctgg ggagaccgcg cgccgccgcc aagcttccac
    10801 gaaaaccggg gcgtcaggtc gcccagtgcc agcggattgt ccaacccgca gacccgcagc
    10861 gcctcgatcc cggccgtcgg gggagcggca aaggcgcgca acggcgcggc gactgcactc
    10921 gatccggcaa cggcaatccc aatggcctcg cgcctgttga ctttcatgct tcctgtcccc
    10981 tgtccgtttc ctgttggttt acggcttgat tgtctgtctc ttcagtacga cttgcgcgct
    11041 caacaccggt tccgccgccg atctggccag ctccaccttg gccgtgccgg ggcgcacctc
    11101 ccagcggcgt gcacgggcat tgaaatccgc cagcaggcga gggtcggccg tcagcgaaat
    11161 ctcgcggctt tcgcccgggt tcagcaccag cttgtcccag cccaccagcc gccgcgcctt
    11221 gccggcaagc gtaacgtaga cctgcggcac gtcggcgccg gggcgctggc cggtgttggt
    11281 gacggtgaag ctcgcgctca gcgtgcgccc gccggtcacc ttcagcccgg aatagccgaa
    11341 agatgtgtag gacaggccat ggccgaacgc gaacagcggc ttctcgccct tgcggtcgaa
    11401 ccagcggtag cccacatccg atccctcggg ataagtgatc gagaacggcg cgctgttggt
    11461 gttcaggccg taagtagcct tgtcctcctt gcttggcgga ggcaggtccg cgcccggaaa
    11521 cttcgggttg ggcaactggt caacgcttac cgggaaagtc accggcaggc ggccggacgg
    11581 attgaccttg ccggtgataa tctcggcaat ggcctcgccg ccgcgctggc cggaatacca
    11641 cgccgaaaga accgcgggca ccttgcccaa ccacggcatg gcgacggcat tgcccgtttc
    11701 cagcaccacc accgtgcgcg ggttgccact ggcgacctgg tcgatcagcg catcctgggc
    11761 gccgtcgagc gaaaggtcgc gctggtcttc ggcttcggtg ctgaacttcg tggcgaacac
    11821 gatggccatg tcggccgcct ttgccgccgc cgccgcttcc gcgggctggc tcccgtcgac
    11881 gaaggtcacg ttcaccccgg ggagcgcatc gcgcagcgcc gccaggggga cggaaccgcc
    11941 ccacgccttg cgggcaaacg acgcggccgg gccgcccagc agccgttcgg tcgcgcggaa
    12001 tccgcccact ggcacaacct ggctcgatcc gccgccagag gggacgccca tgtccgcctt
    12061 gccgccaatc acgactacag accgtacggt cctggccagc ggcagcaggc cgccctcgtt
    12121 gcgcagcagc accgtgccct cgcgagcggc ttccagtgct acttcgccgt tcttcgcata
    12181 gtcgatcggc gtctgcccgc gcggcgtgat gtcggcgcga ttggcataca tcgaccagag
    12241 gattcgcttc acggcggtgt cgaccgcgct ggcgggaatg gtcccatccg ccagccgcgg
    12301 cttcagttcg gttgagaacc agcgcttctt gtcgatcagc tcgcccgact gctggtcgag
    12361 ccccgccaac agggcctcgg tcgaatggac cgcgccccag tccgacatga cgaagccctt
    12421 gaagcccca
//
LOCUS       DATNBV010000363         2668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_514411,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000363 DATNBV010000000
VERSION     DATNBV010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514411"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..2095
                     /locus_tag="VN627_08650"
     CDS             95..2095
                     /locus_tag="VN627_08650"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HXD03362.1"
                     /translation="MSDDLFDKLPAASGEAYDGSAIEVLEGLEPVRRRPGMYIGGTDE
                     RAFHHLAAEVLDNAMDEAVAGHANRIEVTLEEGNRLTIGDNGRGIPVDEHPKYPGKSA
                     LEVILTTLHSGGKFSGKAYATSGGLHGVGVSVVNALSTLTRIEVARNKELYAQEFSRG
                     LPTGPLQKIGNAPNRRGTQVTFVPDAEIFGAEAAFKPARLFRLVRSKAYLFAGVEIRW
                     KCAPSLASEDVPAEATFQFPGGLADHLAEQLGARECVTTQFFRGSQDFPPNQQGEGEQ
                     GRVEWAIAWPLWSDGSYSYYCNTIPTPDGGTHEQGLRAALTKGIRAFGELTGNKKAKD
                     IAPEDVITGSEVMLSVFIRDPQFQSQTKDRQTSPEAARMVENAVRDHFDHFLTDNMER
                     GKALLGYVMERMDERLRRKAEREVKRKTATNARKLRLPGKLTDCSGEGSGETELFIVE
                     GDSAGGSAKQARDRKTQAILPIRGKILNVASATADKIRANSEIADLILALGCGSRKDC
                     NPDNLRYDRIIIMTDADVDGAHIATLLMTFFFQEMPEVVRRGHLYLAQPPLYRLTAGS
                     TSAYARDDLHRAELEATMFKGKKVEVSRFKGLGEMNPGQLRETTMSPASRGLLRITLP
                     EQNEQRFAVKDLVDRLMGRDPAQRFMFIQNRAGEVDRELIDA"
     gene            complement(2096..>2668)
                     /locus_tag="VN627_08655"
     CDS             complement(2096..>2668)
                     /locus_tag="VN627_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD03363.1"
                     /translation="LGGSAAITEFADSTEEQWRYDLDINVMTMLRTTRAVLPGMIARR
                     RGGRIINIASTAGLVGDVYLASYSAAKAAMYGFTVTLAMETGRHEITVNAVAPYATVP
                     ANPAEQASRGSRFNPVTGMFANPSADTLAMAEQISRKTVLPRSAAFTDEIGGAAVYLA
                     SRQAAFVTGETIVVDGGIRLGWRHPETPAG"
BASE COUNT          480 a          991 c          799 g          398 t
ORIGIN      
        1 cgtgtcgctg tgaggccaag atggccccac acctgacgat aacccccaac ccccgccttg
       61 cgcccggaat cccgccgctc ctataaccgg tcccatgtcc gatgatctct tcgacaagct
      121 ccccgccgcc agcggcgaag cctatgacgg ttccgcgatc gaggtgctcg aagggcttga
      181 gccggtccgc cgccgcccgg gcatgtatat cggcggcacg gacgaacgcg ctttccatca
      241 cctcgctgcc gaagtgctcg acaatgccat ggacgaggcg gtggccggcc atgccaaccg
      301 gatcgaggtc acgctcgagg aaggcaaccg cctgaccatc ggcgacaacg gccgcggcat
      361 cccggttgac gagcatccca agtatcccgg caaatcggcg ctcgaggtga tcctcaccac
      421 gctccactcg ggcggcaagt tttccggcaa ggcctatgcc acctccggcg ggcttcacgg
      481 cgtcggcgtc tcggtggtca atgccctgtc gaccctcacc cgcatcgagg tcgcccgcaa
      541 caaggagctc tacgcgcagg aattctcgcg cgggctcccc accggcccgc tccagaagat
      601 cggcaatgcg cccaaccggc gcggcaccca ggtcaccttc gtgcccgatg ccgagatctt
      661 cggcgccgaa gccgcgttca agccggcccg actgttccgc ctcgtccgtt ccaaggccta
      721 tctcttcgcc ggggtcgaga tccgctggaa atgcgccccc tcgctcgcca gcgaggacgt
      781 gcccgctgaa gccaccttcc agttccccgg cggcctggcc gatcacctcg ccgaacagct
      841 cggtgcccgc gagtgcgtga ccacgcagtt cttccggggc tcccaggact ttccgccaaa
      901 ccaacaagga gagggggagc agggccgggt cgaatgggcc atcgcctggc cgctgtggtc
      961 cgacgggtca tattcctatt actgcaacac catcccgacg ccggatggcg gcacccacga
     1021 acagggcctg cgcgccgcgc tcaccaaggg catccgcgcc ttcggagagc tcaccggcaa
     1081 caagaaggcc aaggacatcg cgccggaaga cgtgatcacc ggcagcgagg tcatgctctc
     1141 ggtcttcatc cgcgatcccc agttccagag ccagaccaag gaccgccaga ccagccccga
     1201 agccgcgcgc atggtcgaga acgccgtgcg cgatcacttc gaccatttcc tcaccgacaa
     1261 catggagcgc ggcaaggcgc tcctcggcta tgtcatggag cgcatggacg aacggcttcg
     1321 gaggaaagcg gagcgcgagg tcaagcgcaa gacggcgacc aacgcccgca agctgcgcct
     1381 ccccggcaag ctcaccgact gctcgggcga aggcagcggc gagaccgagc tgttcatcgt
     1441 cgaaggcgat agcgctggcg gcagcgccaa gcaggcgcgt gaccgcaaga cccaggcgat
     1501 cctcccgatc cgcggcaaga tcctcaacgt cgccagcgcc acggccgaca agatccgcgc
     1561 caactcggaa atcgccgacc tgatcctcgc cctcggctgc ggctcgcgca aggactgcaa
     1621 ccctgacaat ctgcgctacg accgcatcat catcatgacc gacgccgacg tcgacggcgc
     1681 ccacatcgcc accctgctga tgaccttctt cttccaggaa atgcccgagg tcgtccgccg
     1741 cggccacctc tacctcgccc agcccccgct ctaccggctc accgccggct caacctcggc
     1801 ctacgcccgc gacgatctcc accgcgccga gctcgaggcg acgatgttca agggcaagaa
     1861 ggtcgaagtc tcccgcttca agggcctggg cgagatgaat ccgggccagc tgcgcgaaac
     1921 aaccatgtcc cccgccagcc gcggcctgct gcgcatcacc ctgcccgagc agaacgagca
     1981 gcgtttcgcc gtgaaggacc tggtcgaccg cctgatgggc cgcgaccccg cccagcgctt
     2041 catgttcatc cagaaccgcg ccggcgaggt cgatcgcgag ctgatcgacg cctgatcagc
     2101 ccgccggcgt ctccggatgc cgccagccca gccggatccc gccgtccacc acgatcgtct
     2161 cgccggtgac gaaagccgcc tgccggctgg cgaggtaaac cgccgcgccg ccgatctcgt
     2221 cggtaaaggc cgcactgcgc ggcaacacgg tcttgcgcga gatctgctcg gccatcgcca
     2281 aggtatccgc gctcggattg gcgaacatgc ccgtgaccgg gttgaaccgg ctgccgcggc
     2341 tggcctgctc ggccgggttg gccggcactg ttgcataggg cgcgacggca ttgacggtaa
     2401 tttcatgccg ccccgtctcc atggccagcg tcacggtaaa cccgtacatc gctgccttgg
     2461 ccgccgaata gctggcaagg tagacatcgc ccaccagccc cgcggtcgat gcgatgttga
     2521 tgatccggcc gccgcgccgc cgcgcgatca tcccgggcag cacggcccgg gtcgtgcgca
     2581 gcatggtcat gacattgatg tcgaggtcgt aacgccattg ctcctcggtg ctgtcggcaa
     2641 actcggtgat cgccgcgctg ccgcccag
//
LOCUS       DATNBV010000364         9744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4043622,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000364 DATNBV010000000
VERSION     DATNBV010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9744
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4043622"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VN627_08660"
     CDS             complement(<1..1173)
                     /locus_tag="VN627_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXD03364.1"
                     /translation="MKLVDWKQAKLLAGAAALALAACSADPETLLARARQEYASHNFK
                     AAQLDLATVLKARGSDPAVLELHARTALALGDGESARASLEKLPAANRPADHALLLGE
                     AALLRRDGKAADAAVAADQSAEAWRVRAMAALLRGDDAAAETAFAKGEGAPGPQARLL
                     ADHARLRLHKGDLAGAQALVARALKADANSLDARLMEARAAVAAGDLGRAVALYDGVG
                     KAWPGNLAALIGKAGVLGDRGRLKEMKEVVAQAGSAGVNAADLAWLQARLAAAEGDWK
                     GARDILQANDEALADRSEAALLHAQVLTKLGQPEQARARLQPVLTRDPGNLAVRRALA
                     EAAMAARDPAGAVEVLRPLAALPTASTADLRLLAAAAQQAHSPDAAAFAARAKFPPP"
     gene            complement(1268..2137)
                     /locus_tag="VN627_08665"
     CDS             complement(1268..2137)
                     /locus_tag="VN627_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXD03365.1"
                     /translation="MVMTAAEGQLAFAPRPGGIGADVASADFSRPFTPAQVAALKEAV
                     ARYRVLFFHGQHIDSATHLARARQFGELEIHPFAREKAGRVIFSDPDEPALVVVESLP
                     GKPSVAEKWHSDVTFMTTPSMGSLLRMTHRPESGGNTRWADMAAAYRGLDEETRRLID
                     DAVAVHDWHIFRRNMAGLPEEEQRLEAMRAQMPPVEHPVVRTHPLTGERLIYVNRNFT
                     VRIKGMAEEASADLLEYLYAQAENPAYHAELEWEVDDIAFWDNRITQHSVKGDVVGHR
                     RLERVSIVGDRPF"
     gene            2278..3378
                     /gene="ychF"
                     /locus_tag="VN627_08670"
     CDS             2278..3378
                     /gene="ychF"
                     /locus_tag="VN627_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXD03366.1"
                     /translation="MGFRCGIVGLPNVGKSTLFNALTETQAAQAANYPFCTIEPNVGN
                     VGVPDPRLDQLAAIAGSQKIIPTQLGFVDIAGLVRGASKGEGLGNQFLGNIREVDAIV
                     HVLRCFENDDIQHVDNRVDPISDAETVETELMLADLESLEKRVPAAQKKAAAGDKEAK
                     VIASVLGQALELLREGKPARLTQPKDIEEARVFAQAQLLTAKPVLYVCNVEEESAAQG
                     NDFSARVFGKAKAEGANAVIVSAAIEADLVGMDMDERMVFLEEMGLHETGLNRIIRSG
                     YDLLHLITFFTVGPKEARAWTVEKGSKAPQAAGAIHSDFERGFIRAETIAFADYVALG
                     GEAGAKDAGKLRAEGKEYVVQDGDVLHFRFNV"
     gene            3386..3859
                     /locus_tag="VN627_08675"
     CDS             3386..3859
                     /locus_tag="VN627_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXD03367.1"
                     /translation="MSGEKLYFEDLVVGSVETLGALPVIHEEVIDFARKFDPQGFHLD
                     DAAAAASPFGRLAASGWQTGAMTMRLLVDHFLARAASLGGAGIEQLLWVKPVYPGDTL
                     SLRLTLLDKRRSKSRPEMGLIWHLVETLNQHGEVVMSQKTTGMMAVRDPSVPVAG"
     gene            complement(3860..4378)
                     /locus_tag="VN627_08680"
     CDS             complement(3860..4378)
                     /locus_tag="VN627_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03368.1"
                     /translation="MRTTTWAGLIAAMALVTACQREKTPAEQAAEDARAVAMVEAAQQ
                     VKAPPVPIEPQPITAADSEQNGFYGAGCTLVPTSVPGGDPVIMANDRRALVKFAGKFV
                     TFAADPGSPSLSLGVHSHYVGKAQSLWLAKGQGDGTALGREGMRWDGRVEIRDAQDRT
                     IWSSAGALTCGS"
     gene            complement(4378..6261)
                     /gene="mutL"
                     /locus_tag="VN627_08685"
     CDS             complement(4378..6261)
                     /gene="mutL"
                     /locus_tag="VN627_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HXD03369.1"
                     /translation="MPVIRRLPETLVNRIAAGEVVERPASALKELVENAIDAGAGQIA
                     VRLGNGGLDLIEVTDDGCGMAPEEIALALERHATSKLPDEAIELVATLGFRGEALPSI
                     ASVARLTIESRPRGSDGWSRMVDHGVLAGEGPAALPPGTRIRVEDLFGRVPARRKFLR
                     SARSEYAACQDVVRRLAMARPDIGFTLEHDGRRVMVVQGGEGLANRVAGLVARELASE
                     GIAIEAERGFARLTGVAGLPTFNRGVADHQYLFVNGRPVKDRLLIGAVRGAYADMLAR
                     DRHAVLALFLQVPPEEVDVNVHPAKTEVRFRDPAFVRGFIVSGLRHALDSAAQRSARP
                     AATGAMANWQAEPLEFNATATPLPQAGGAPFYTPLPQAGGVGGGQAYQNQPLTTLFAR
                     QHTQPRSNVSEAGRLWRSYEADVLAPAARAEEAQPLPEQAADFPLGVARGQVAQTYIV
                     AEAADGLVIVDQHAAHERLVLERLKAAGAGQAAAAAQALLIPEVVELEEADCDRLEEA
                     APRLADHGLALERFGPDAMLVRAVPAPLAGGDIKALVRDVADDIAANGDALLLGERLD
                     HVLATMACHGSVRAGRALSVAEMNALLREMERTPRSGQCNHGRPTWVKLAHGDIEKLF
                     GRK"
     gene            6480..7526
                     /locus_tag="VN627_08690"
     CDS             6480..7526
                     /locus_tag="VN627_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXD03370.1"
                     /translation="MSSLVSRFFKLGSQNLAIDLGTANTLVYAQDRGIVLNEPSVVAI
                     ETINGIKKVKAVGDDAKMMMGKTPDNIEAIRPLRDGVIADIEIAEEMIKHFIRKVNGK
                     RSMFRYPEIVICVPSGSTSVERRAIRDAASNAGASQVFLILEPMAAAIGADMPVTEPV
                     GSMVVDIGGGTTEVAVLSLRGLAYTTSVRVGGDKMDEAIVSYVRRHHNLLIGEATAER
                     IKKDYGCAVIPADGVGETIHIKGRDLVNGVPKEITITQANIAEALSEPIGAIVEGVRI
                     ALENTAPELAADIVDQGIVLTGGGALIRGLDDYLREETGLPVSIAEDPLSCVALGTGR
                     AMEDPIYRGVLMTA"
     gene            7628..8581
                     /gene="mreC"
                     /locus_tag="VN627_08695"
     CDS             7628..8581
                     /gene="mreC"
                     /locus_tag="VN627_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HXD03371.1"
                     /translation="MAPPNARRTGFSKRAQYGTFFGYLAAIAGGLVGAVVLVVSLLDP
                     TAFAGLRGLAADAAAPAGRVAAAGRMGTQTVAADLWGYLTWGPRVAKMDRELELARVR
                     LAEAGSQAEENRRLKALLGLADAEAKPVAFARLINSTASSPRRYATLSVGRDHGVTTG
                     MPVRSPEGVIGRVLEVAATTARVLLVTDPESLVPVMRARDGVPAFAQGKGDGTLQLRL
                     INLGLNPLRPGDVFVTSGSGGIYTPGMAIGVVVSLTRDGAVVRVLSDPTQSAFVAVYP
                     AYAVAAKMPEALVPVPEPVPPAKKKLPKTPKPASKTAGKAH"
     gene            8581..9120
                     /gene="mreD"
                     /locus_tag="VN627_08700"
     CDS             8581..9120
                     /gene="mreD"
                     /locus_tag="VN627_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HXD03372.1"
                     /translation="MPWAGDAEAHQAGRLNRAPSPLLARSLPWLVIAFGSILQTLPVI
                     ASAPIMPPFGFLFLLAWRQLRPGLLPVWAGLPLGFVDDLYSGQPLGSAMLLWSLAMIV
                     FEVIEQRFPWRSYVIDWLVGAAVIAGYLVLASAIARISGAVNVLAPQLVVSVLVFPLA
                     ERFIAICDRTRLWRFRMLG"
     gene            9165..>9744
                     /locus_tag="VN627_08705"
     CDS             9165..>9744
                     /locus_tag="VN627_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXD03373.1"
                     /translation="MMGRHISSGTLANSFDRRSVVIGALQGGVGMLLAARMGWIALVE
                     GEKYRTLSESNRVNLSLIPPRRGWILDRHGLPMATNRTDFRVDLIPERLEDPDKTLAE
                     LGQLLSLTPVKLQDLSDKIKRSRGFQPVEVATKLDMDAFSAVTVRMPELPGVIPQRGF
                     SRFYPTGPAVAHLIGYVGPASAEDYEKDRNPLL"
BASE COUNT         1479 a         3159 c         3520 g         1586 t
ORIGIN      
        1 cggagggggg aatttcgccc gtgcggcaaa tgccgccgca tcagggctgt gggcctgctg
       61 ggcggcggcg gcgagcaggc gcaggtctgc cgtgctggcc gtgggcaagg ccgccagggg
      121 ccgcagcacc tcgacggcac cggcggggtc cctggccgcc atggccgctt ccgcaagggc
      181 gcggcgcacg gcgagattgc cgggatcgcg ggtcagcacc ggctgcaggc gggcgcgggc
      241 ctgttcgggc tgcccgagct tcgtgagcac ctgcgcatgg agcagagcgg cctcgctgcg
      301 atcggccaag gcttcgtcat tggcctgaag tatatcgcgc gcgcccttcc agtccccttc
      361 ggccgcagcc aatcgcgctt gcagccaggc gagatcggcg gcgttcacgc cggcgctgcc
      421 ggcctgcgcc acgacctcct tcatctcctt gaggcggccc cggtcgccga gcacgccggc
      481 cttgccgatg agggcagcca gattgccggg ccaggccttg ccgacgccat catagagggc
      541 gacggcccgc ccgaggtcgc cggcggcgac ggccgcgcgg gcttccatca gccgggcgtc
      601 cagcgaattg gcatcggcct tgagcgcccg ggcaaccagc gcctgggccc ccgccagatc
      661 ccccttgtgg aggcggaggc gggcatggtc ggcgagcagg cgggcctgcg ggccgggagc
      721 gccctccccc ttggcgaagg ctgtttccgc ggcggcgtcg tcgccgcgca gcaaggccgc
      781 catggcgcgt acccgccatg cttcggcaga ctgatccgcg gcaacggcgg catcggccgc
      841 cttgccatcg cggcgcagca gggccgcttc gccgagcagc agcgcatgat cggccgggcg
      901 attggcggcg ggcagctttt ccagcgaggc gcgcgcgctt tcgccatcgc cgagggccag
      961 ggcggtgcgg gcatgcagtt ccagcacggc gggatcgctg ccgcgcgcct tcagcaccgt
     1021 ggcaaggtcg agctgggctg ccttgaaatt gtgcgaagca tattcctgcc gggcacgggc
     1081 cagcagcgtt tcgggatcgg cgctgcacgc ggccagggcc aatgccgccg ctccggccag
     1141 cagtttggcc tgtttccaat caaccagttt cacgagcgcg cagatcccca attgccgaga
     1201 ggggacattg tgcagccgag gattgaaaat tcggtgaaaa ggggccgggc ccgatcagaa
     1261 tgcgcgttca gaacgggcga tcgccgacaa tgctgacccg ttcgagccgg cggtggccga
     1321 ccacgtcgcc ctttacggaa tgctgggtga tacggttgtc ccagaaggca atgtcgtcca
     1381 cttcccattc cagttcggca tgataggccg ggttctcggc ctgggcataa agatattcga
     1441 gcaggtcggc gctggcttcc tcggccatgc ccttgatccg cacggtgaag ttgcggttca
     1501 cgtagatcag ccgctcaccg gtgagcggat gagtgcgcac caccggatgt tcgaccggcg
     1561 gcatctgcgc gcgcattgct tcgagccgct gttcttcctc gggcaggccg gccatgttgc
     1621 ggcggaagat gtgccagtcg tgtacggcga cggcatcgtc gatcaggcgg cgggtttcct
     1681 cgtcgagccc ccggtaggcg gcggccatgt cggcccagcg ggtgttgccg ccgctttcgg
     1741 gccggtgggt catgcgcagc agcgagccca tggacggcgt ggtcatgaag gtgacatcgc
     1801 tatgccactt ttcggcgacc gacggcttgc ccggcaggct ttccaccacg accagcgcgg
     1861 gctcgtcggg atcggaaaag atcacccgcc cggccttctc ccgcgcgaag gggtggatct
     1921 cgagttctcc gaactggcgg gcccgggcca ggtgggtcgc cgaatcgata tgttggccgt
     1981 ggaagaacag cacccggtat cgagcgacgg cttccttcag cgcggcgacc tgggccgggg
     2041 tgaacgggcg ggagaaatcg gcgctggcca cgtcggcgcc gatgccgccc gggcgcgggg
     2101 cgaaggccag ctggccctcg gcggcggtca tgaccatggg atctctccgg aattcctagt
     2161 tttcatggcg atgatccggt tcgatggcgg gcaagtcaag gcgccccgcg cggcgaactg
     2221 gccaaacgcc cccctccccg ctatggcgcg cggaaattca gtcatccgga gtttgaaatg
     2281 ggtttccgtt gcgggatcgt gggcttgccc aatgtcggca agtccacgct gttcaatgcg
     2341 cttaccgaga cgcaggccgc ccaggcggcg aactatccgt tctgcacgat cgagcccaat
     2401 gtcggcaatg tcggcgtgcc cgatccgcgg ctcgaccagc tggcggcgat cgccgggagc
     2461 cagaagatca tcccgaccca gctgggcttc gtcgacattg ccggcctcgt gcgcggggcg
     2521 agcaagggcg aagggctggg caaccagttc ctcggcaata tccgcgaggt ggatgccatc
     2581 gtccacgtgc tgcgctgctt cgagaatgac gacatccagc atgtcgacaa ccgggtcgat
     2641 ccgatctcgg acgcggagac ggtggaaacg gagctgatgc tggccgatct ggagagcctc
     2701 gagaagcgcg tgccggcggc gcagaagaaa gcggcggcgg gggacaagga ggcgaaggtg
     2761 atcgcctcgg tgctcggcca ggcgctggag ctgctgcgcg agggcaagcc ggcgcggctg
     2821 acgcagccca aggacatcga ggaggcgcgc gtgtttgccc aggcgcagct gctgaccgcc
     2881 aagcccgtgc tttacgtgtg caatgtggag gaagagagcg cggcgcaggg caatgatttc
     2941 tccgcgcggg tgttcgggaa ggccaaggcc gagggcgcca atgcggtgat cgtttcggcc
     3001 gcgatcgagg ccgatctcgt gggcatggac atggacgagc ggatggtctt ccttgaggag
     3061 atgggcctgc acgagaccgg gctgaaccgc atcatccgtt cgggctatga cctcctccac
     3121 ctgatcacct tcttcaccgt gggccccaag gaggcccgcg cctggaccgt ggaaaagggt
     3181 tccaaggctc cgcaggcggc cggtgcgatc cactccgatt tcgagcgcgg gttcatccgg
     3241 gccgagacca tcgcctttgc cgactatgtt gcgctgggcg gcgaagcggg cgcgaaggac
     3301 gctggcaagc tgcgggcgga aggcaaggaa tacgtggtgc aggacggcga cgtgctgcac
     3361 ttccggttca acgtctgagg gcgcgatgag cggggaaaag ctgtacttcg aggacctggt
     3421 cgtcggctcg gtggagacgc tgggcgcgct gccggtgatc cacgaggagg tgatcgactt
     3481 tgcccgcaag ttcgatcccc agggttttca cctcgacgat gcggcagcag ccgcttcgcc
     3541 gttcgggcgg ctggcggcca gcggctggca gaccggcgcc atgaccatgc gactgctggt
     3601 cgaccatttc ctcgcccggg ccgcctcgct gggcggcgcg gggatcgaac agctgctctg
     3661 ggtgaagccg gtctatcccg gggacacgct gagcctgcgg ctgacgctgc tggataagcg
     3721 gcgcagcaag tcgcgccccg aaatggggct gatctggcac ctggtggaga cgctcaacca
     3781 gcatggcgag gtggtcatgt cgcagaagac gaccgggatg atggccgtgc gcgacccttc
     3841 cgtaccggtg gccggctgat cagctgccgc aggtgagcgc gccggcgctg gaccagatgg
     3901 tgcggtcctg cgcgtcgcgg atctcgaccc ggccgtccca gcgcattccc tcgcggccga
     3961 gcgccgtgcc gtcgccctgc cccttggcca gccagagcga ctgggccttg ccgacgtagt
     4021 gggaatggac gccgagcgaa aggctggggc tgccgggatc ggcggcgaag gtgacgaact
     4081 tgccggcgaa cttgacgagg gcccggcggt cattcgccat gatcacggga tcgccgccgg
     4141 gaacgctggt cggcacgagg gtgcagccgg cgccgtagaa gccgttctgc tcgctatcgg
     4201 cggcggtgat cggttggggt tcgatgggga ctggcggggc cttcacctgc tgcgcggcct
     4261 cgaccatggc gacggcgcgg gcatcctcgg cagcttgctc ggcgggcgtt ttctcgcgct
     4321 gacaggcggt gacgagggcc atggctgcga tcaaaccggc ccacgtcgtc gtccgcatca
     4381 ctttctcccg aacagtttct cgatatcccc gtgggcgagc tttacccagg tgggccggcc
     4441 gtggttgcac tggccggacc gcggcgtgcg ttccatctcg cgcagcaggg cgttcatttc
     4501 cgcgaccgac aacgcacggc ccgcccgaac ggatccatgg caggccatgg tcgccagcac
     4561 gtggtcgagc cgttcgccga gcaggagggc atcgccattg gcggcgatat cgtcggcgac
     4621 gtcgcggacc agggccttga tgtccccccc ggcgagcggc gcggggacgg cgcggacgag
     4681 catggcgtcg gggccgaagc gttcgagggc gaggccgtga tcggcgaggc gcggggcggc
     4741 ttcctcgagc cggtcgcagt cggcttcctc gagttcgacg acttcgggaa tgagcagggc
     4801 ctgggcagcg gcagcggcct gcccggcccc ggcggccttg aggcgttcga gcaccaggcg
     4861 ttcgtgggcg gcgtgctggt cgacgatgac caggccgtcg gccgcttcgg cgacgatgta
     4921 ggtttgcgcc acctggccgc gggcgacccc gagtgggaag tccgccgcct gttcgggcag
     4981 gggctgggcc tcttcggcac gggcggcggg ggcgaggaca tcggcctcgt agctgcgcca
     5041 gaggcggccg gcttccgaga cattcgatcg tggctgggtg tgttggcggg cgaagagggt
     5101 ggtgagcggt tggttttggt aggcttgccc acccccaacc cctcccgcct gcgggagggg
     5161 agtgtaaaag ggagcccctc ccgcctgcgg gaggggggta gcggtagcgt tgaattccag
     5221 cggttcggcc tgccagttgg ccatggcgcc ggtggccgcg ggccgggcgc tgcgctgggc
     5281 ggcgctgtcg agggcgtggc gcaggcccga aacgatgaag ccgcggacga aggcgggatc
     5341 gcggaagcgg acttcggtct tggcggggtg gacgttgacg tccacttcct cgggcgggac
     5401 ctggaggaac agggcgagca cggcgtgacg atcccgtgcg agcatgtcgg cataggcacc
     5461 gcgcacggcg ccaatgagca ggcggtcttt caccgggcgg ccatttacga acaggtactg
     5521 gtgatcggcc acgccgcggt tgaaggtggg caggccggcc acccccgtca gcctggcgaa
     5581 accgcgctcc gcctcgatgg cgatgccttc gctggccagt tcgcgggcga ccaggccggc
     5641 gacgcgattg gccagccctt ccccgccctg caccaccatg acgcggcggc catcgtgttc
     5701 gagggtgaag ccgatgtccg ggcgggccat ggcgaggcgg cggaccacgt cctggcaggc
     5761 ggcgtattcg ctgcgggcgg agcgcaggaa cttgcggcgg gcagggacgc ggccgaacag
     5821 gtcttccacg cggatgcggg tgccgggcgg caaggcggcc gggccttctc cggcgaggac
     5881 gccatgatcg accatgcgcg accagccatc gctgccgcgc gggcggcttt cgatggtcaa
     5941 ccgcgcgacg ctggcgatgg agggcagtgc ctcgccccgg aaaccaaggg tggcgaccag
     6001 ctcgatagct tcgtcgggca gcttggaggt ggcgtggcgt tcgagcgcga gggcgatctc
     6061 ctccggcgcc atgccgcagc cgtcgtccgt cacctcgatc aggtcgagcc cgccgttgcc
     6121 gagccggacg gcgatctggc cggccccggc atctatggcg ttttccacca gctccttcag
     6181 cgccgaggcc gggcgctcca ccacctcgcc ggcggcgatg cggttgacca gcgtttcggg
     6241 caggcggcga atgacgggca tgggctgggg tgtgcggact ctgcagcagg aaggcgcaag
     6301 gctagcggcg aactgccagc ttttcgagac gaggagaaag catctccacg gctttcgcga
     6361 caaaattttg gctttgcggc gaaccgtgcg ctaaggagcc gcgtccccaa cctcgccgga
     6421 ccgcatgcca ctgccgtggc agcgggcggt ggcgaccgaa aaaccacgga aatccatcga
     6481 tgtcttcact ggtgtcccga ttcttcaagc ttggctcgca gaaccttgcc atcgacctcg
     6541 gcaccgcgaa tacgctggtc tacgcgcagg accgcgggat cgttctcaat gaaccttcgg
     6601 tggtcgcaat cgagacgatc aacgggatca agaaggtcaa ggcggtcggc gacgacgcca
     6661 agatgatgat gggcaagacg ccagacaaca tcgaggccat ccgccccctg cgcgacggcg
     6721 tcatcgccga catcgaaatc gccgaagaga tgatcaagca cttcatccgc aaggtgaacg
     6781 gcaagcgctc gatgttccgc tacccggaga tcgtgatctg cgtgccctcg ggcagcacct
     6841 cggtcgaacg ccgcgcgatc cgcgatgcgg cctccaatgc cggggccagc caagtgttcc
     6901 tgatcctcga gccgatggct gccgcgatcg gcgccgacat gccggtgacc gagccggtcg
     6961 gctcgatggt ggtcgacatc ggcggcggca ccaccgaggt ggccgtgctg tcgctgcggg
     7021 gcctggccta taccacttcg gtccgcgtcg gcggggacaa gatggacgaa gccatcgtgt
     7081 cctacgtgcg ccgccaccac aacctgctga tcggcgaagc cacggctgag cggatcaaga
     7141 aggactacgg ctgcgcggtg atcccggcgg acggcgtggg cgagacgatc cacatcaagg
     7201 gccgcgacct cgtcaacggc gtgcccaagg aaatcaccat tacccaggcg aacatcgccg
     7261 aggctctttc cgaaccgatc ggcgccatcg tcgaaggcgt gcgcatcgcg ctggaaaaca
     7321 ccgcgccgga actggccgcc gacatcgtcg accagggcat cgtcctcacc ggcggcggtg
     7381 cgctgatccg ggggcttgac gactatctgc gcgaggaaac cggtctgccg gtttcgatcg
     7441 cggaagatcc gttgtcctgc gtggcgctgg gtacgggccg ggccatggaa gacccgatct
     7501 atcgcggcgt gctgatgacg gcctgaaccg gttgggctat tcgagagttc gggcgtgacg
     7561 ggcaggcttt cgggagcctg cagtgggacc acggccgcga gacgcaaggt gaggtaagga
     7621 gcagggcatg gcgccgccga acgcccgtcg caccggcttt tccaaacggg cgcagtacgg
     7681 gaccttcttc ggctacctgg cggcgattgc cggcgggctg gttggcgcgg tcgtgctggt
     7741 cgtctcgctg ctcgacccga cggcctttgc cggcctgcga gggctggctg ccgatgctgc
     7801 ggccccggcc gggcgggtgg ccgctgccgg ccggatgggc acccagaccg tggctgccga
     7861 cctgtggggc tatctcacct gggggccgcg cgtcgcgaag atggatcgcg agctcgaact
     7921 agcccgggtg cggctggccg aagcgggcag ccaggccgag gaaaaccgcc gcctgaaggc
     7981 cctgctcggc cttgccgatg ccgaggccaa gccggttgcc ttcgcccgcc tgatcaattc
     8041 cacggcttcg agcccgcgcc gctatgccac gctctccgtg ggccgcgacc acggagtgac
     8101 caccggcatg ccggtgcgct cgcccgaagg ggttatcggc cgggtgctgg aagtggcggc
     8161 gaccacggcg cgggtgctgc tggtcaccga tccggaaagc ctggttccgg tgatgcgcgc
     8221 gcgcgatggc gtgccggcct ttgcccaagg caagggcgat ggcaccttgc agctgcggct
     8281 gatcaacctt ggcctcaatc cgctgaggcc tggcgacgtg ttcgtgacct cgggctcggg
     8341 cgggatctac acgccgggca tggctatcgg cgtggtcgtt tcgctcaccc gcgacggggc
     8401 agttgtccgc gtgctcagcg acccgacgca gagcgccttt gtcgcggtct atcccgctta
     8461 tgccgttgcg gcgaagatgc cggaagcact cgtgccggtg cccgaaccgg tgccgccggc
     8521 caagaagaaa ctccccaaga cgcccaagcc cgcctccaag accgccggga aggcccactg
     8581 atgccctggg cgggtgacgc cgaggcgcac caggccgggc ggctcaaccg tgcgccctcg
     8641 cccttgctgg cccgcagcct gccgtggctg gtgatcgcct tcggctcgat cctccagacg
     8701 cttccggtga ttgcctcggc gccgatcatg ccgcccttcg gtttcctctt cctgctggcc
     8761 tggcgccagc tgcggccggg actgcttccc gtctgggcgg ggctgccgct ggggtttgtc
     8821 gacgatctct acagcgggca gccgctgggt tcggccatgc tgctgtggtc gctggcgatg
     8881 atcgtgttcg aggtgatcga acagcggttt ccgtggcgct cctacgtgat cgattggctg
     8941 gtgggcgccg cggtcatcgc cggttatctg gtgctggctt cggccatcgc ccggatcagc
     9001 ggcgcggtga acgtgctggc gccgcagctg gtggtgtcgg tgctggtctt cccgctggcc
     9061 gagcggttca tcgccatctg cgaccggacg cgcttgtggc gcttccgcat gctcggttag
     9121 cgcctgccgt gttcaaacgc ttcaagcgca gccgcgcccg ccagatgatg gggcgccaca
     9181 tctcctcggg cacgctggcc aacagcttcg accggcgcag cgtggtgatc ggcgcgctgc
     9241 agggcggcgt cggcatgctg ctcgccgcgc ggatgggctg gatcgcgctc gtcgagggcg
     9301 agaaatatcg caccctttcg gaaagcaacc gggtcaacct ttcgctgatc ccgccgcgcc
     9361 ggggctggat cctcgatcgc cacggcctgc cgatggcgac caaccggacc gactttcgcg
     9421 tcgacctcat tccggagcgg cttgaagacc cggacaagac cctggcggag ctgggccagc
     9481 tgctcagcct gacgccggtc aagcttcagg acctttcaga caagatcaag cgcagccgcg
     9541 gcttccagcc cgtggaagtg gcgaccaagc tcgacatgga cgccttttcc gccgttaccg
     9601 tgcgcatgcc ggaattgccc ggggtgatcc cgcagcgtgg tttctcccgg ttctatccga
     9661 ccgggccggc cgtggcgcat ctcatcggct atgtcggccc ggcctcggcc gaggactacg
     9721 agaaggaccg caatccgctg ctga
//
LOCUS       DATNBV010000365        15438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2132502,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000365 DATNBV010000000
VERSION     DATNBV010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 15438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15438
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2132502"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="VN627_08710"
     CDS             <1..1520
                     /locus_tag="VN627_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXD03374.1"
                     /translation="ALVCTASLDLGVDWGDIDLVVQMGAPKGSSRLLQRIGRANHRLD
                     CPSKALLVPGNRFEFLEAMAAREAALAGQRDGEDFRPGALDVLAQHVMACACAGPFGE
                     DELLAEVRSAEAYSWVDEAVWARVLAFVATGGYALRAYDRFQRIVRAKDGTWRLAHPE
                     QAHRHRLNAGIIVDAEMLDVRFRNGKSLGRVEEAFGASLSPGDTFRFAGMDLEVESVR
                     DLELVVRAARKSAMIPSYMGARIPISTHLAERVRRLLVDRAGWSRFPDDVREWLEVQD
                     WRSRLPGPGELLVESFPHHGRHYSVIYTFEGWNANQSLGMLITRRMEERGLGPLGFVA
                     NDYALGVWGLKPIEDPAPLLSPDILTHEFIAWVEDSHLLRRAFREVAVIGGLVERQHP
                     GKRKSGRQVTFSTDLIYDVLRRHEPDHLLIEAAWADARTRLTDVGRVADVLERAAEQI
                     VHVRLDRVSPLAVPVLVMIGRESLPAGAADEDLLLEAESLAGEAMRMEEAETEGD"
     gene            complement(1638..2096)
                     /locus_tag="VN627_08715"
     CDS             complement(1638..2096)
                     /locus_tag="VN627_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXD03375.1"
                     /translation="MAMSGGKDDGSPMMEMNTTPLIDVMLVLLIMFIITIPVATHAVN
                     IDLPSPNPPPNNIKIDTIKNKVVLTYDNKVLWNGQPVNNGQFVALLQQTLQMKPEPEL
                     QFQPEAGASYELSAAALNIIKASGVSKFGFVGNEQYSEFGKAPNAPKATP"
     gene            complement(2137..2670)
                     /locus_tag="VN627_08720"
     CDS             complement(2137..2670)
                     /locus_tag="VN627_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXD03376.1"
                     /translation="MAISMGAGGEEKPMSDINTTPLVDVMLVLLIIFLIAVPVAIQQI
                     EKLRIPIMVSQESKDKVENLLLSVTTTDEAGRSAGDPGYEGASRNGECRIYFNNITPV
                     DSAELRDLAYKRLDTIVKREGGPEALRANPEKVPQVHIRGDVNAPWRCVAGAIYNVQI
                     SGYPTVGFISNPVDPNG"
     gene            complement(2789..3556)
                     /locus_tag="VN627_08725"
     CDS             complement(2789..3556)
                     /locus_tag="VN627_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXD03377.1"
                     /translation="MLIDLLAAAANAAPQNKFGFKEALEQGGFIAQATVVILGIMSFG
                     SFFILFTKWFEQSKILKQGGDLRTGFWRAGSIKEGTAKLEKNSAWRQIAEDGLAAEEQ
                     HSKMAQATDAHDWLVGSLTRSEASINARLASGLPFLATVGATAPFIGLFGTVVGIYRA
                     LIAIGLAGSASIDKVAGPVGEALIMTALGLLVAVPAVLAYNYLQARNKRIAEQLQSFA
                     TDVLANINSNGAIKPAAVPAAAAKAAPAKPAAAPAKP"
     gene            complement(3620..4267)
                     /locus_tag="VN627_08730"
     CDS             complement(3620..4267)
                     /locus_tag="VN627_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXD03378.1"
                     /translation="MAYADQSMSGSKITALIIVALIHVVVGYALVTGLAYSAAKQILK
                     KVTTVDVKEEEKPKEPPPPPPKVVAPPPVVVPPPKVNVAVSAPVIDVVPVAPPPPPPV
                     LVPPAPAAPPPPRFTPKGAAPKGNPGNWATANDYPSRALREEREGTTGFRVTVGPDGR
                     VTDCQITSSSGSPDLDQTACEKIRQRARFTPATDGEGNPTSGTYSNRVRWVIPKD"
     gene            4607..5521
                     /locus_tag="VN627_08735"
     CDS             4607..5521
                     /locus_tag="VN627_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03379.1"
                     /translation="MKRRSPGTPFPSLAGMVFAAGLLLSQPANSAVSGPGAGGTPTLA
                     PPPAQGFAANSLTYADLVDLADHSPSVLHVMIRKVVPVEPARAPGVRPGWVRMYVEAR
                     PLALLAGPPLPADSLRYLVDVPLDAKGKPPKLPRLAKRGVILFARTLAERPGELQLVA
                     PDAQILWDESVESRLKAILADLLAPGAPGRITGVREAIYVPGTLAGEGETQLFLSAAN
                     GEPASITVVHQPGRAARWSASFSEVLDSTGEPPARETLAWYRLACFLPASLPPRANVS
                     SMPADGAQAAADYRMVRESLGVCPRSRS"
     gene            5637..6944
                     /locus_tag="VN627_08740"
     CDS             5637..6944
                     /locus_tag="VN627_08740"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HXD03380.1"
                     /translation="MAEPLRIALAGLGTVGAGVIRLLETNAGLIARRAGRPLQIVAIS
                     ARDRTKKRGVDLAPYAWEDDMVILGERPDVDVVVELVGGSDGPALACARTTIEAGKAL
                     VTANKAMIAHHGLELAAKAEAARVALKFEAAVAGGIPVIKGLREGAAANAIERVYGIL
                     NGTCNFILSTMEDTGRDFGDVLAEAQSRGYAEADPTFDIEGTDAAHKLSILAAIAFGA
                     KIDFAGVDTAGISRLRAADIAQADSLGYVIRLIGMAEPDHSGPVPRLFQRVQPHLVPC
                     DHPLAHVDGATNAVVAEGNFSGRLLFQGAGAGDGPTASAVVADLIDIARGEIGAPFSV
                     PVAELEALDRASTGHRTGRAYVRFIVADRPGVLAEITAAMRDAGVSIESLIQKGSTGV
                     DGEVLVALVTHEGPESAVTEALRLLEGSPSLSSPPLVMQILGH"
     gene            7078..8055
                     /gene="glpX"
                     /locus_tag="VN627_08745"
     CDS             7078..8055
                     /gene="glpX"
                     /locus_tag="VN627_08745"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HXD03381.1"
                     /translation="MTDTPASKVLDRVLVLEMVRVTEAAAIGAAKLIGRGDEKAADAA
                     AVEAMRAAFNELYMDGTVVIGEGERDEAPMLYIGEKVGAAIGTGPRIDIALDPLEGTT
                     ICAKAGPNALAVLAVAEEGGLLNAPDVYMDKLAVGPGYPEGIISLAKSPSENVSAVAA
                     AKGVDPSEIMVCVLDRPRHAELIGELRGLGCGVVLIGDGDVAGVIAVTDPETNIDLYM
                     GQGGAPEGVLAAAALRCVGGQFNGRLVFRNDDEKGRARKWGITDLDKIYTRDELAKGD
                     CIFAATGVTDGSMLKGVKHHKNGMLTTESIVMRASSGTVRRVAGEHRRK"
     gene            8209..9144
                     /locus_tag="VN627_08750"
     CDS             8209..9144
                     /locus_tag="VN627_08750"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXD03382.1"
                     /translation="MKIMAGNSNLPLARAIAGYLELPLTEASVRRFADEEVFVEIHEN
                     VRGEDVFVVQSTSYPCNDNLMELLICIDALRRASAKRITAVVPYFGYARQDRKPGPRT
                     PISAKLVANLITTAGADRVLAVDLHAGQIQGFFDIPTDNLYAAPVMAADIQARYGGQP
                     LMVVSPDVGGVVRARALAKRLDNAPLAIVDKRRDKPGQSEVMNIIGEVKGRACIMIDD
                     IIDSGGTLCNAAQALMDAGAASVTAYITHGVLSGAAVSRVNASALKELVISDTIQPTD
                     ATKESDRVRVIAIAPLIGEAMRRIADESSVSSLFD"
     gene            complement(9181..10305)
                     /locus_tag="VN627_08755"
     CDS             complement(9181..10305)
                     /locus_tag="VN627_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXD03383.1"
                     /translation="MAEKPLRVGIVSGNFGMYAHKPGWEACPGVEVAAVCTSREETAR
                     QAAAQNGVPKAYWNHAEMARDPDLDIIVIGTKPNLRREMVLEAIRNGKHVFAMAPFAA
                     NLEAAREMRDAARAAGVVAAVDSVMPWHGANRFIRDELVSGRAGKPISVEARFVINLF
                     NGNPNGDYWQWFGTRSNGASAVRNFGTHTLHLLVYLLGEVEAVSALTTTALKEWHSAD
                     GTIRRSQVDDTAQVLLRFANGTMGTMTLAWCSAPISGWRMELMAEHVSYRTEDPGYFP
                     TNADLQLFRGERTQGFVPMAVPSAYPSVPDERFRTALGAAQAWDIAQAAQDFVRAIRL
                     GGAPLPDFETGYHVEAVLEAISRASEQRRWINVAEVAAGG"
     gene            complement(10516..11604)
                     /locus_tag="VN627_08760"
     CDS             complement(10516..11604)
                     /locus_tag="VN627_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXD03384.1"
                     /translation="MKKLILLSAVASAALASPALARDKTWYIEGDAGATIVENTDAVF
                     STPPAITVDTKTGYDFGGIVGYDFGMFRLEAEASYRRAGIKSTTTGTTVLTNSTTPAV
                     SGKVDALAFMANALIDIGPDDGLQGFIGGGVGVARAGVKALTGATVDVDDTDSRFAWQ
                     ALAGVRYPLSNNVDIGLKYRYFNQTGIKMQDVTGDPFSTKIRSHSLMATLAYNFGAAP
                     PPPPPPPPPPPPPPPPPPPPPPPPPPPVCNKGPYIVFFDWDKSDITPEAATILDSAIT
                     AYGNCQSVPIMLAGYADRSGTPKYNLGLSNRRNASVQTYLTGHGVPAAAITAQGFGEA
                     NPRVPTADGVRELQNRRVEITYGPGSGM"
     gene            complement(11898..12818)
                     /locus_tag="VN627_08765"
     CDS             complement(11898..12818)
                     /locus_tag="VN627_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXD03385.1"
                     /translation="MDEAIAPANGTTREGQPISYNRAPAPDLAPWIARFYVSDVLAPD
                     NHLLECGLFNDIAMLRIQLKGQWTALAADGAHANGRAALFFGPQSRRMPVAVTGSFIS
                     IGAGLRPGTGHAVMGVSAADFRDRIVPCDALGLPGSHALDQLETVSDPEARLRLLEDL
                     IRAIVRQPGVAEPDPVTQRFELLAFADPSASIAEFARDCAITQRQLERIILRDFGMPP
                     KQVLRRARALDMASHLRGVADQAEAEELALRYYDQSHLIRDFTQLFGMSPSQFVARPQ
                     PILTLALESRQARRLELLERVPPGGQRPWL"
     gene            13031..13813
                     /locus_tag="VN627_08770"
     CDS             13031..13813
                     /locus_tag="VN627_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXD03386.1"
                     /translation="MQFDDDIALAHRLADAAAQAIRPWFRSDAGSERKDDATPVTKAD
                     RAAEEAIRRILMAEVPRDTVIGEEFGTTPGSSGRSWVIDPIDGTAGFLAGRPLFGTLI
                     ALVVEGWPVLGVIDQPILGERWVGASGRPTTLNGQPARTRLCRQLADATLATTGPHYF
                     DDHDGAHFMSLAAKTDHRRMVMGGDCYNYAMLASGHLDVVCEANLKLHDWAALVPVVE
                     GAGGTMCDWNGDPLHAGSDGRVLALGDPARLDDVVEALACAH"
     gene            13830..14255
                     /locus_tag="VN627_08775"
     CDS             13830..14255
                     /locus_tag="VN627_08775"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HXD03387.1"
                     /translation="MRLFFYGTLLQGTGNRIAVALHRHLAKGRPATTHGRLYALPDPG
                     GWYPALLPDPAAPLVHGMVHESLASFTPGLLADVDAYEGCTGSTGEYRRESIVIATVE
                     GAILAEAYVYNVPLPPGAVALPHGSFTRFIAEGGLVAYC"
     gene            14389..14592
                     /gene="rpmI"
                     /locus_tag="VN627_08780"
     CDS             14389..14592
                     /gene="rpmI"
                     /locus_tag="VN627_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HXD03388.1"
                     /translation="MPKMKTKSGVKKRFKLTATGKVKHGVAGKRHRLISHNGKYIRQN
                     RGTEVISDADAKVIKKWAPYGLN"
     gene            14606..14968
                     /gene="rplT"
                     /locus_tag="VN627_08785"
     CDS             14606..14968
                     /gene="rplT"
                     /locus_tag="VN627_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXD03389.1"
                     /translation="MSRIKRGVTTRAKHKRILDQAKGYRGRRKNTIRVARQAVEKAGQ
                     YAYRDRKVKKRTFRALWIQRINAAVREAGLTYSQFIHGAKLAGIALDRKAMADLAMNE
                     GAVFTAVIAQAKAALPAA"
     gene            15179..>15438
                     /locus_tag="VN627_08790"
     CDS             15179..>15438
                     /locus_tag="VN627_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXD03390.1"
                     /translation="MRFFEPPEPLRRFFTSFYLVEIDRYVGETDHLHPEWANLRFFSG
                     GCPVAEGANGDRVERVRFCATGPSSRSVRFSLGPTRMWGIGLL"
BASE COUNT         2516 a         5111 c         5157 g         2654 t
ORIGIN      
        1 gggcgctggt gtgtaccgcc agcctcgatc tcggggtgga ctggggcgat atcgacctgg
       61 tcgtccagat gggcgcgccc aagggctcca gccgcctcct ccagcggatc ggccgggcca
      121 atcaccggct cgattgcccg agcaaggccc tcctcgtccc cggcaaccgc ttcgaatttc
      181 tcgaagccat ggccgcccgc gaagctgcgc tcgccggtca gcgcgatggc gaggacttcc
      241 gcccaggcgc gctcgacgtc ctggcgcagc acgtcatggc ctgcgcctgc gccggcccct
      301 tcggggagga tgagctgctg gccgaggtcc gctccgccga ggcctacagc tgggtcgacg
      361 aggcggtctg ggcgcgcgtc ctggccttcg tcgcgaccgg cggctatgcg ctccgggctt
      421 acgaccgctt ccagcgcatt gtccgcgcga aggacggcac atggcgctta gcccaccccg
      481 agcaagctca ccgccaccgc cttaacgccg ggatcatcgt cgatgccgaa atgctcgacg
      541 tgcgcttccg caacggcaag tccctgggcc gggtcgagga agccttcggg gcgagcctct
      601 ccccgggcga taccttccgc ttcgccggga tggacctcga agtggaatcg gtccgcgatc
      661 tcgagctcgt cgtgcgcgcg gcgcgcaagt cggcgatgat cccaagctac atgggcgcgc
      721 gcatcccgat ctcgacccac ctcgccgagc gcgtccgccg cctgctggtc gaccgcgccg
      781 ggtggtcgcg cttccccgat gatgtgcgcg aatggctcga ggtgcaggac tggcgctcgc
      841 gcctgccggg gccgggcgaa ctgctggtcg aaagcttccc ccaccacggt cgccactatt
      901 cggtgatcta caccttcgag ggctggaatg cgaaccagtc gctcggcatg ctcatcaccc
      961 gccgcatgga agagcgtggc ctcggccccc taggattcgt cgccaacgac tacgccctcg
     1021 gcgtctgggg gctgaagccg atcgaggatc cggcgccgct gctttcgccc gatatcctca
     1081 ctcacgaatt catcgcctgg gtcgaggatt cgcacctgct gcgccgggcc ttccgggaag
     1141 tcgccgtgat cggtgggctg gtggagcggc agcacccggg gaaacgcaag tcgggccgcc
     1201 aggtcacgtt ctcgaccgat ctcatctacg acgtgctgcg ccgccatgag cccgatcacc
     1261 tgctgatcga agctgcctgg gccgatgcgc gcacccggct caccgatgtc ggccgcgtcg
     1321 cggacgtgct cgaacgcgcg gctgagcaga tcgtccacgt ccggctggac cgggtttcgc
     1381 ccctggccgt cccggtgctg gtcatgatcg gtcgcgaaag cctgcctgcc ggtgctgccg
     1441 acgaggacct gttgctcgag gccgaaagcc tggcaggcga ggccatgcgg atggaagagg
     1501 cggaaaccga gggagactga ccggtccggt aaacgtggcc actgtaacgt caaacggcgc
     1561 cgcgccacca gctccgtcca caaaaaaggg ggcggatcgc tccgccccct gttcttgaaa
     1621 ggtctgtcgc cagcagctta cggcgtggcc ttgggcgcgt tgggcgcctt gccgaattcc
     1681 gaatactgct cattgccgac gaagccgaac ttgctgacgc ccgaggcctt gatgatgttc
     1741 agggccgcgg ccgacagctc gtagctggcg ccggcttccg gctggaactg cagttccggt
     1801 tccggcttca tctgcagcgt ctgctgcagc agggccacga actgcccgtt gttcaccggc
     1861 tgcccgttcc acagcacctt gttgtcgtag gtgagcacga ccttgttctt gatcgtgtcg
     1921 atcttgatgt tgtttggcgg cggattgggc gacggaaggt cgatattcac cgcgtgggta
     1981 gcaacgggga tggtgatgat gaacatgatg aggagcacga gcatgacgtc gatgagcggc
     2041 gtcgtgttca tttccatcat cggcgagcca tcatccttgc cgcctgacat tgccatggga
     2101 acttactcct gaataacggt tggcgcagct tgtgcgtcag ccgttcgggt cgaccgggtt
     2161 ggagatgaag ccgacggtcg gatagcccga gatctgcacg ttgtagatgg cgccggccac
     2221 acagcgccac ggggcgttga cgtcgccgcg gatgtgcacc tggggaacct tctccggatt
     2281 ggcgcgcaga gcttccgggc cgccttcccg cttcacgatc gtgtcgagcc gcttgtacgc
     2341 gaggtcgcgc agctcggccg aatcgaccgg cgtgatgttg ttgaagtaga tgcggcattc
     2401 gccgttgcgc gaggcacctt catatcccgg gtcgccagca ctgcggccgg cctcgtcggt
     2461 ggtggtcacg ctcagcagca ggttctcgac cttgtccttc gattcctgcg agaccatgat
     2521 tggaatacgc agcttctcga tctgctggat cgcgaccgga accgcgatga gaaagatgat
     2581 cagcagcacc agcatcacgt ccaccaacgg ggtcgtgttg atgtccgaca tcggcttttc
     2641 ttcgccgccg gcgcccatcg aaatggccat gagttacatc ctatcctgta atcgggcccg
     2701 gtaaggatgc gaaccggcat gccgcggttc gcttcctgac cggccgggtg gaactgccgc
     2761 gaggcagtcc cactgacggc agtgctgctt acggcttggc cggggcagcg gccggcttgg
     2821 ccggagcggc cttggccgca gccgcgggga cagcggcagg cttgatcgcg ccgttcgagt
     2881 tgatgttggc cagaacgtcg gtggcgaagc tctggagctg ctcggcgatg cgcttgttgc
     2941 gggcctggag gtagttgtag gcaagcacgg cggggacggc gaccagcagg ccgagcgcgg
     3001 tcatgatgag cgcttcaccg accgggccgg cgaccttgtc gatcgaggcc gaaccggcga
     3061 gaccgatggc gatcagcgcg cggtagatgc cgaccacggt accgaacaga ccgatgaacg
     3121 gcgcggtggc gcccacggtc gcgaggaacg gcaggcccga ggcgaggcgg gcgttgatcg
     3181 aggcttccga gcgggtcagc gagccgacca gccagtcatg cgcgtcggtg gcctgggcca
     3241 tcttcgagtg ctgctcctcg gcggccaggc cgtcttcggc gatctggcgc catgcgctgt
     3301 ttttctcgag cttggcagtg ccttccttga tgcttccggc gcgccagaag cccgtacgca
     3361 gatcgccgcc ctgcttcagg atcttgctct gctcgaacca cttggtgaac aggatgaaga
     3421 acgaaccgaa cgacatgata cccaggatga cgacggtggc ctgggcgatg aagccgccct
     3481 gctcaagcgc ttccttgaag ccgaacttgt tctgcggggc tgcgttcgcg gcagcggcaa
     3541 gaaggtcaat aagcatgcga tagtcctctc aagaataagg tgggtgtcgg gccgcgcttg
     3601 ggccggcccg gaaaaatgat cagtccttcg ggatcaccca gcggacgcgg ttggaatagg
     3661 tgcccgaggt ggggttgccc tcaccatccg tcgcgggcgt gaaccgggcg cgctggcgga
     3721 tcttttcgca ggcggtctgg tcgagatccg gactgccgct cgagctggtg atctggcaat
     3781 cggtcacccg gccatcgggc ccgacggtga cgcggaagcc ggtcgtgccc tcgcgctctt
     3841 cgcgcagggc gcgcgaagga tagtcgttgg cggtcgccca gttgccgggg ttgcccttgg
     3901 gcgcggcacc cttgggggtg aaccgcggcg gcggcggcgc ggccggggcg ggcggcacca
     3961 gcacaggcgg cgggggcggc ggagccacgg ggaccacgtc gatcaccggc gcggacaccg
     4021 caacgttgac cttcggcggc ggaaccacga ccggcggcgg cgcaaccacc ttcggcggcg
     4081 gcggcggcgg ctccttcggc ttttcctctt ccttgacgtc taccgtcgta acctttttca
     4141 ggatctgctt cgccgccgaa taggcgaggc ccgtgaccaa ggcataaccg acgacgacgt
     4201 gaatcaatgc tacgatgatc agtgcggtaa tcttgctacc gctcatcgat tggtcagcgt
     4261 aagccattca gacgcatcac tccatttcag ggccccgaac aaccagggca accagaccct
     4321 cgcatgtcgc catgcgcgaa cccggaattc ttaattttcc accctgccgg tcggtccggg
     4381 gcgccgcatg gacccccttg cccgagcgga cgcggattgg gccgccctga ccgggcgtgc
     4441 cacttttgcg tgagctttag cgtcggactg ccacccacgc aacggcagat tgcccccttg
     4501 cctcgggtta acctgcaatt cacaaccatt aatatcaaaa ccgactgctt gatgcgcact
     4561 acgcacaaat gtgcttgtcg ctcccggtga aattggagtt agcctgatga agcgccgcag
     4621 ccccggcacc ccctttccat ccctcgccgg aatggtcttc gcagcggggc tgttgttgtc
     4681 gcaacccgcc aattccgcgg tctccggccc aggagcgggc gggacgccca ccctcgctcc
     4741 gccgcccgcg cagggcttcg cggccaacag tctgacctat gccgacctgg ttgacctggc
     4801 cgatcattcg cccagcgtgc tccatgtcat gatccgcaag gtggtgccgg tcgaacctgc
     4861 ccgcgcgccg ggcgtgcgcc cgggctgggt gcgcatgtat gtcgaggcgc ggccgctggc
     4921 gctgctcgca ggtcctccgc ttccggccga ttcgctgcgc tatctggtcg acgtgccgct
     4981 cgacgccaaa ggcaagccgc ccaagcttcc gcgtctggcc aagcgcgggg tgatcctctt
     5041 tgcccggacc ttggccgagc ggcccgggga actccagctc gttgcgccgg atgcccagat
     5101 cctgtgggac gaatcggttg aatcgcggtt gaaagcaatc ctcgccgatc tgctggcccc
     5161 cggagcgccc ggacggatca ccggggtgcg cgaggcgatc tacgtgcctg gaacgcttgc
     5221 cggcgagggt gagacccagc tgttcctcag cgcggccaat ggcgaacccg cctcgatcac
     5281 cgtggttcac cagccgggca gggctgcgcg gtggagcgcc tcgttctccg aagtgctgga
     5341 ttccacgggc gaaccgcccg cgcgggagac gctcgcctgg tatcgcctcg cctgtttcct
     5401 tccggccagt ctgccgcccc gcgccaatgt ttcgtcaatg cccgctgacg gcgcgcaggc
     5461 ggcggccgat taccggatgg ttcgcgaatc gctcggggtc tgtccgcgca gccgcagctg
     5521 accgcgccct tcgctgcctc gggcgaagag catcgcggcc tctggccttt cgccgcgccg
     5581 tgtcctaggg ccccaccccg aacggggccg ccgcacgggg cccgaaaggg atgaacatgg
     5641 ccgaaccgct tcgtattgcg cttgccgggc tgggtaccgt gggggctggc gtcattcgcc
     5701 tgcttgagac caacgccgga ctgatcgccc gccgcgccgg ccgtcccctg cagatcgtgg
     5761 cgatctcggc gcgcgaccgc accaagaagc gcggcgtcga tcttgccccc tacgcctggg
     5821 aagatgacat ggtcatcctg ggcgaacggc ctgatgtcga cgtcgtggtc gaattggtcg
     5881 gcgggtcgga tggcccggcg ctcgcctgcg cgcgcacgac catagaagcc ggcaaggccc
     5941 tggtcaccgc caacaaggcg atgatcgccc accacggcct ggaactcgcc gccaaggccg
     6001 aagcagccag ggtcgcgctc aagttcgagg cggcggtggc ggggggcatt cctgtcatca
     6061 agggcttgcg cgagggcgcc gccgccaacg ccatcgagcg ggtctacggc atcctcaacg
     6121 gcacctgcaa tttcatcctc tcgaccatgg aggataccgg gcgcgacttc ggcgatgtcc
     6181 tggccgaggc gcagagccgc ggatacgcag aggccgatcc gaccttcgac atcgagggga
     6241 cggacgccgc ccacaagctg tcgatcctgg ccgccattgc cttcggcgcg aagatcgatt
     6301 tcgccggtgt cgacacggcc ggcatctcgc gcctgcgcgc ggccgatatc gcccaggccg
     6361 attcgctcgg ctacgtgatc cgcctcatcg gcatggccga gcctgatcac agcggtcccg
     6421 tcccgcgcct gttccagcgc gtccagccgc acctggtgcc ttgcgaccat ccgctcgctc
     6481 acgtcgacgg ggcgaccaat gcggtcgtgg cggagggcaa tttctccggc cggctcttgt
     6541 tccagggcgc cggtgccggc gacgggccga cggccagcgc cgtcgtggcc gacctcatcg
     6601 acattgcccg cggcgaaatc ggtgcgccat tctccgtgcc tgtcgccgaa ctcgaggccc
     6661 tggaccgcgc gtctaccggg catcgcaccg gccgcgccta tgtgcgcttc atcgttgccg
     6721 atcggccggg tgttctcgcc gagatcaccg cggcgatgcg cgacgccggg gtttcgatcg
     6781 aaagcctgat ccagaagggc agcaccggag tggatggcga agtgctggtt gccctggtca
     6841 cccacgaggg cccggaaagc gcggtcaccg aggcgctgcg cctgctggag ggttcgccca
     6901 gcctgtcgag cccgccgctc gtcatgcaga tcctcggcca ctgagtgcac cggcggagcg
     6961 ctttgctccg cgacctcggc acctcgcatt cgtatgcgca aagtggggcg cggcactcga
     7021 cagcgcccga tcccctgtct aagcgggcgg cgaactcgcg cagaacaggg attccccatg
     7081 accgataccc ccgccagcaa ggtgctcgac cgcgtcctcg tgctcgaaat ggtccgcgtg
     7141 accgaggcgg ccgccatcgg cgcggccaag ctgatcggcc ggggtgacga gaaggccgcc
     7201 gacgctgcgg cggtggaagc catgcgcgcg gctttcaacg agctctacat ggatggcacg
     7261 gtggtcatcg gcgagggtga acgcgacgag gccccgatgc tctatatcgg cgagaaggtg
     7321 ggtgccgcca tcggcaccgg gccgcgcatc gacatcgcgc tcgacccgct cgaaggcacc
     7381 accatctgcg ccaaggcggg accgaatgcg ctggcagttc tcgccgtggc ggaagaaggc
     7441 ggcctgctca acgcgcccga cgtctacatg gacaagctgg ccgtcggacc cggctacccc
     7501 gagggaatca tcagcctcgc caagagcccc agtgaaaacg tcagcgccgt tgccgcggcc
     7561 aagggcgtcg atccgtcgga aatcatggtc tgcgtgctcg atcgcccgcg ccatgccgaa
     7621 ctgatcggcg aactgcgcgg cctcggctgc ggcgtggtgc ttatcggcga tggcgacgtg
     7681 gcgggcgtga tcgcggtcac cgatcccgaa accaatatcg acctctacat gggtcagggc
     7741 ggcgcgccgg aaggcgttct ggccgcggcg gccctgcgct gcgtgggcgg acagttcaat
     7801 ggccgtctcg tcttccgcaa cgacgacgag aagggccgcg cccgcaagtg gggcattacc
     7861 gatctcgaca agatctacac ccgcgacgaa ctggccaagg gcgattgcat cttcgctgcc
     7921 acgggggtta ccgacggctc gatgctcaag ggtgtcaagc accacaagaa cggcatgctc
     7981 accaccgaat cgattgtcat gcgcgcgtcg tcaggcacgg tccgccgcgt ggccggggaa
     8041 caccgccgca agtaagtccg ccgcgcctgt cggggggcgc ggcatggaat cggcgtccgt
     8101 gggggccagg ctaccggcgg tttttccccg ccgcggggcc cgccaatgtt gcaattgcgt
     8161 gaccctcagc taggagcccc tcgtggatac ggcggaaggg aatcgcgcat gaagatcatg
     8221 gccggcaaca gcaacttgcc cctcgcacgg gcgattgcgg gctatctcga gctgccgctc
     8281 accgaagcca gcgtgcgccg cttcgccgac gaggaagtct tcgtcgagat tcacgaaaac
     8341 gtgcgcggcg aggatgtgtt cgttgtccag tccacgtcct atccctgcaa tgacaacctg
     8401 atggagctgc tgatctgcat cgatgcgctg cgccgcgcct cggccaagcg gatcaccgcg
     8461 gtggttccct acttcggcta tgcccggcaa gaccgcaagc ccgggccgcg cacgccaatc
     8521 tcggccaagc tggttgccaa cctgatcacc acggccgggg ctgaccgcgt tctcgcggtc
     8581 gatctccacg ccggccagat ccagggcttc ttcgatatcc cgaccgacaa tctctatgcc
     8641 gccccggtca tggctgccga catccaggcg cgctatggcg gccagccgtt gatggtggtc
     8701 tcgcccgacg tcggcggcgt ggttcgcgcc cgggccctcg ccaagcggct ggacaacgcc
     8761 ccgctcgcca tcgtcgacaa gcggcgcgac aagcccggcc agtccgaggt catgaacatc
     8821 attggcgagg tcaagggccg tgcctgcatc atgatcgacg acatcatcga ttcgggcggc
     8881 accctgtgca acgccgccca ggccctgatg gatgccggcg ccgcctcggt caccgcctac
     8941 atcactcacg gcgtcctctc cggcgcggcg gtcagccggg tcaatgcatc ggcgctaaag
     9001 gaactggtga tctccgacac catccagccg accgatgcca ccaaggagag cgatcgcgtc
     9061 cgcgtcatcg cgatcgcccc gctgatcggc gaggcgatgc gacggatcgc ggacgagagc
     9121 tccgtatcca gtctgttcga ctagggggcc tgacccaagg ctggctcagg gctgcggcga
     9181 tcagccgccc gcagccactt cggccacatt gatccagcgg cgttgctcgc tggcgcggct
     9241 gatcgcttcg aggacggcct ccacgtgata gccggtctcg aagtcgggca ggggggcgcc
     9301 gccaaggcgg atcgcgcgca cgaagtcctg cgccgcctgg gcaatgtccc aggcctgggc
     9361 tgcgccaagt gccgtgcgga accgttcatc cggaactgag ggataggccg aggggaccgc
     9421 catgggcacg aagccttgcg tccgttcgcc gcgaaacagc tgcaggtccg cgttggtggg
     9481 gaaatagccg gggtcttcgg tgcggtagct cacgtgctcg gccatgagct ccatgcgcca
     9541 gccgctgatc ggggcggagc accaggccag cgtcatcgtg cccatggtgc cgttggcgaa
     9601 gcgcagcagg acctgcgccg tgtcatccac ctgtgagcgc ctgatcgtgc catcggcgga
     9661 atgccattcc ttcagcgccg tggtggtcag ggccgacacg gcctcgacct cgccgagcag
     9721 gtagaccagc aggtgcaggg tgtgggtacc gaagttgcga accgccgagg cgccattgct
     9781 gcgggtgccg aaccactgcc agtaatcgcc gttgggattg ccgttgaaca ggttgatgac
     9841 gaacctggct tcgacggaaa tcggcttgcc ggcccggccc gagacgagct cgtcgcgaat
     9901 gaagcggttg gcgccgtgcc agggcatgac cgaatccacc gcggccacca cgcccgctgc
     9961 ccgcgccgca tcgcgcattt cccgtgctgc ctcgaggttg gcggcgaatg gcgccatggc
    10021 gaagacgtgc ttgccgttgc ggatcgcttc cagcaccatc tcgcgccgca ggttgggctt
    10081 ggtgccgatc acgatgatgt cgaggtccgg atcgcgggcc atctccgcgt gattccagta
    10141 ggccttcggc acgccgttct gcgccgccgc ctgccgtgcc gtttcctcgc gcgaggtgca
    10201 gaccgcagcc acttccacgc ccgggcaggc ttcccagccg ggcttgtggg catacatgcc
    10261 gaaattgcca ctgacgatgc cgaccctgag cggtttctca gccatgttgc agatccttcc
    10321 cgattagttg gccctttggg cgatggggaa aggctatcgc aattgccggc agcgtgaagg
    10381 ttcgttccac cattagcatg gatgggctgg cattccggcc agaacccgct cctccgcggg
    10441 cgcacaggga accggcaaag aaaaaggggc cggattgctc cggcccccaa ttctggttca
    10501 acgctaacgc gctgattaca tgcccgaacc cggaccgtag gtgatttcca cccggcggtt
    10561 ctgcagttcg cggacgccgt cggcggtcgg cacgcgcggg ttggcttcgc cgaagccctg
    10621 cgcggtgatc gcggcggccg gcacgccgtg gccggtcaga taggtctgaa ccgaggcgtt
    10681 gcggcggttc gagaggccga ggttgtactt cggcgtaccc gaacggtcag catagcccgc
    10741 gagcatgatc ggaacgctct ggcagttgcc gtaagcggtg atcgcgctgt cgaggatcgt
    10801 cgcggcctca ggcgtgatgt ccgacttatc ccagtcgaag aacacgatgt acggaccctt
    10861 gttgcacacc ggcggcggag gcggcggagg cggcgggggc ggcgggggag gcggcggcgg
    10921 cggcggcggc ggcggcgggg gcggcggcgg cggagccgcg ccgaagttgt acgccagggt
    10981 cgccatcagc gagtgcgagc ggatcttggt ggagaacgga tcccccgtca cgtcctgcat
    11041 cttgatgccg gtctggttaa agtagcgata cttcagcccg atatcgacat tgttgctgag
    11101 cgggtagcgc acgccggcca gagcctgcca ggcaaagcgc gagtccgtgt cgtcaacatc
    11161 gacggtggcg ccggtcagcg ccttcacgcc ggcccgggcc acgcccacgc cgccgccgat
    11221 gaagccctgc aggccgtcgt cgggaccgat gtcgatcagc gcgttcgcca tgaacgccag
    11281 ggcgtcgacc ttgccgctga cggcgggggt ggtcgagttg gtcagaacgg tggtgccggt
    11341 cgtggtcgac ttgatcccgg cccgacgata gctggcttca gcctcgaggc ggaacatgcc
    11401 gaaatcgtaa ccgacgatcc cgccgaaatc ataaccggtc ttggtgtcga ccgtgatcgc
    11461 gggcggcgtc gagaacacgg catcggtgtt ttcaacgatg gtcgcaccgg catcgccttc
    11521 gatgtaccag gtcttgtcgc gcgcgagcgc cgggctggcc agtgccgcgg atgcgacggc
    11581 cgagagcagg atgagttttt tcatgttccc ctcgtgagac gtggatgcgg cagattccct
    11641 agcgatctgc cggttcgctg gcaatgccga tgttacttat acgttccctg aaccacccgg
    11701 cgcaaacggt tcggtctgtt ggcaatcatc aaggtcactg acggcaatat gcgttgttgc
    11761 gaagacttgg gggattattt ccctgtggca gaacggttgg aaaccggctg gttcctgccg
    11821 ttgcgcgcat caatccggga gatcgccggt tctgtcggaa ccagaggtgt ggctgatctg
    11881 caacatcgga ataaattcta tagccacggg cgctgcccgc caggcgggac ccgctcgaga
    11941 agttccaatc gccgggcctg gcgggattcc agcgcaaggg tcaggatcgg ttggggccgc
    12001 gccacgaatt ggctgggcga catgccgaac agctgcgtga agtcgcggat gaggtggctc
    12061 tggtcatagt agcgcagggc cagttcctcg gcctcggcct gatcggccac gccgcgcagg
    12121 tggctcgcca tgtcgagcgc gcgggcgcgc cgcaggacct gcttgggggg catgccgaag
    12181 tcgcgcagga tgatccgttc cagctggcgc tgggtgatcg cgcagtcgcg tgcgaactcg
    12241 gcgatgctgg ccgaaggatc ggcaaaagcc agcagttcga accgctgggt taccggatct
    12301 ggctccgcga ccccgggctg cctgacgata gcgcggataa gatcctccag cagccgcagg
    12361 cgcgcctcgg ggtcgctcac cgtttccaac tggtccaatg cgtgactgcc gggcagtccg
    12421 agcgcgtcgc agggaacgat gcgatcgcgg aagtcggccg cgctgacgcc catcaccgca
    12481 tggccggtcc cgggccgcag ccctgccccg atgctgatga agctgccggt taccgcgacc
    12541 ggcatccggc gactttgcgg tccgaagaac agggcggcgc gcccgttggc atgggcgcca
    12601 tcggccgcca gcgcggtcca ttggcccttg agctggatgc gcagcatcgc gatatcgttg
    12661 aacagcccgc attcaagcaa atgattgtcc ggtgcaagca cgtcggaaac atagaaccgc
    12721 gcgatccatg gcgcgagatc gggcgcaggc gcacgattgt aggagatcgg ctgcccttca
    12781 cgggtcgtgc cattggccgg cgcaatcgcc tcgtccacgg ccggccgaga cctttttagc
    12841 ataaaacccc gttcaatctc ccccgtcagg acccgagtac ttgcggaaga ctgtgcagac
    12901 aatagacaga ccgtcaccaa gtgtaaaagc ttttactgca gggatttcgt tcaacggccc
    12961 cctctttgtg gtgattggcg cagcgcggca gagcttgacc gccgcccctg cgccggggca
    13021 cagccgcgcg atgcagttcg acgacgatat tgcccttgct caccgcctcg ccgatgcggc
    13081 cgcgcaggcc attcgcccgt ggtttcgcag cgatgctggg agcgagcgca aggatgacgc
    13141 cacccccgtc accaaggccg accgcgccgc cgaggaggcg atccgtcgca tcctcatggc
    13201 ggaggtgccg cgcgacacgg tgatcggcga ggaattcggc acgacccccg gctcgtcggg
    13261 acgctcctgg gtgatcgatc cgatcgatgg cacggccgga ttcctcgccg ggcgaccgct
    13321 gttcggcaca ttgatcgcac tcgtcgtgga aggatggccg gtgctcggcg tgatcgacca
    13381 gccgattctg ggcgagcgct gggttggcgc cagtgggcgg cccactacgc tcaacgggca
    13441 gccggcccgc acccgcctgt gccgtcaact cgccgacgcg accctcgcca ccaccggtcc
    13501 gcactatttc gacgaccacg acggggccca tttcatgagc cttgccgcca agaccgatca
    13561 tcgccggatg gtcatgggcg gcgactgcta caattatgcc atgctggcga gcggccacct
    13621 cgacgtggtc tgcgaggcca acctcaagct gcatgactgg gcagccctgg ttccggtggt
    13681 cgaaggcgcg gggggcacga tgtgcgactg gaacggcgat ccgctccacg ccggctcgga
    13741 cggccgggtg ctggccctgg gcgatccggc gcggctcgac gacgtggtcg aggcgctcgc
    13801 ctgcgcccac tgacctggca gggctggctg tgcggttgtt cttctacggc accctgctgc
    13861 agggcacggg caatcggatt gcagtggcgt tgcaccggca cctcgcgaag ggccgtcctg
    13921 ccacgacgca cgggcggctc tacgccctgc cggacccggg cggctggtat cccgccctcc
    13981 tgcccgatcc ggccgcgccg ctggtccacg gcatggttca cgagagcctc gccagcttca
    14041 ccccggggct cctcgccgat gtcgatgcct atgagggctg caccggaagc accggggaat
    14101 atcgccgcga atccatagtg atagccacgg tcgagggtgc gattttggcc gaggcctatg
    14161 tctataatgt cccgctcccg cccggcgcgg tggcgctgcc ccacgggagt ttcacccgct
    14221 tcatcgccga gggcggcctg gtcgcttact gctgaacctg ccgaatccct tgcgctcggg
    14281 cctgcatacc gctaggggcg cgcccttcat tgacacggaa tcgatgatcg gcctggccaa
    14341 ctagggctgc catggcagat cggacgtgtc tctatcgagg agacgaaaat gcccaagatg
    14401 aagacgaaga gcggcgtgaa aaagcgcttc aagctcaccg ccaccggcaa ggtgaagcac
    14461 ggcgttgccg gcaagcgcca ccgcctgatc agccacaacg gcaagtacat ccgccagaac
    14521 cgcggcactg aagtgatctc cgacgccgat gcgaaggtga tcaagaagtg ggcgccctac
    14581 gggctgaact gaggagcgct gacttatgag ccgtatcaag cggggtgtta ccacccgagc
    14641 caagcacaag cgcattctgg atcaggcgaa gggctatcgc ggccgtcgca agaacacgat
    14701 ccgcgttgcg cgccaggccg tcgaaaaggc cggccaatat gcctatcgcg accgcaaggt
    14761 taagaagcgg accttccgcg ccctgtggat ccagcgcatc aacgctgcgg tgcgcgaagc
    14821 gggcctgacc tattcgcagt tcattcacgg cgccaagctg gccggcatcg cgctggaccg
    14881 caaggccatg gccgatctgg cgatgaacga aggcgcggtg ttcaccgccg tcatcgccca
    14941 ggcgaaggct gcccttccgg ccgcctgatc ctgcgggact gcactatcga aacgggcgcg
    15001 ggcgctggcc gtccgcgccc gttttgcatt tccgggcctg tcgtcggctt cggtaacatc
    15061 tccgatgagc aggccaacgc gggcttgcca gccacccccg tgccgcgata gccctttgat
    15121 cgtgtcaggg cgcatcaggg atcagcagcg gcaatccgca tcgcaagacc cggtacgcgt
    15181 gcgctttttc gagccgccgg agcccctgcg ccgctttttc accagcttct atctcgtcga
    15241 gatcgacaga tacgtcggcg aaaccgacca tctccacccg gaatgggcaa acctgcggtt
    15301 tttctcgggc ggttgcccgg tggccgaagg agcgaacggc gatcgggtcg agcgggtccg
    15361 gttctgtgcg accgggccga gcagccggtc cgtgcgcttc tcgctcggac ccacgcggat
    15421 gtggggcatc ggccttct
//
LOCUS       DATNBV010000366         5684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3045187,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000366 DATNBV010000000
VERSION     DATNBV010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3045187"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VN627_08795"
     CDS             complement(<1..852)
                     /locus_tag="VN627_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HXD03391.1"
                     /translation="MSFPWANEYKPQAPLMQWLDEKLPLPRFVYNAIGAGYPVPRNLN
                     YAWNFGVLAGFCLVLQIVTGVILAMHYAANAGVAFDSVEHIMRDVNWGWMMRYAHANG
                     ASAFFVVVYVHIFRGFFYGSYKAPREMIWLLGVVIFLLMMATAFMGYVLPWGQMSFWG
                     AQVITGLFSAIPLVGDALHTWLLGGFAPDQAALNRFFSLHFLLPFVIAAVVILHIWAL
                     HIPGSSNPTGVDVKDEKDTLPFHPFYTAKDGFGAAVCLLLFALVLYFAPKYLGHPDNY
                     IPANPLAT"
     gene            complement(864..1430)
                     /gene="petA"
                     /locus_tag="VN627_08800"
     CDS             complement(864..1430)
                     /gene="petA"
                     /locus_tag="VN627_08800"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HXD03392.1"
                     /translation="MSDHAGTEAVLPEHGEGVRRRDFINIAAVSAAGVGGVVTLLPLI
                     SQMAPSADVLAESSTELDISAIASGQAIKAVFRKQPVFVRNLTPKEIAEAEAVDISTL
                     RDPQTLKERTKPGKENWLITMGVCTHLGCVPLGAGEGEVKGEFGGYFCPCHGSVYDTA
                     ARIRKGPAPKNLQVPEYAFTSDTVVKIG"
     gene            complement(1670..2443)
                     /gene="pgeF"
                     /locus_tag="VN627_08805"
     CDS             complement(1670..2443)
                     /gene="pgeF"
                     /locus_tag="VN627_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HXD03393.1"
                     /translation="MADTADVARAEGLAAFPHGFLGRTGGVSTGVAASLNVGLGSGDD
                     EAAVAENRRRAAQAIAPGARLVGVYQTHSATCVTVTEPWADDARPEADALVTDRPGLL
                     LGVVTADCAPVLFADRTAGVVGAAHAGWKGALVGVTDAAVAAMVALGARPDRIAAAIG
                     PTIAQASYEVDDAFCDRFLGHDPANAACFAPGRAAHWQFDLPAYLARRLVHAGIGAVE
                     RLGLDTYSQEARYFSFRRSTHRHEAAYGRQISAISLPLA"
     gene            complement(2436..5087)
                     /gene="pepN"
                     /locus_tag="VN627_08810"
     CDS             complement(2436..5087)
                     /gene="pepN"
                     /locus_tag="VN627_08810"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HXD03394.1"
                     /translation="MDIASRPSAPAENPQTADAAPPPAHPPVIRREDYVAPAWLIPEV
                     ALDFTLGLDATQVTSRLSVRRNPAVQAAPLRLAGDGIAARAVRIDGRESNAWRMDGPD
                     LLIDLEGDAHEVSVETAINPAANSHLMGLYASNGMLCTQCEAEGFRRITFHPDRPDVL
                     SVYRVRMSGDKAQFPILLSNGNCVAKGEGESGTHWAEWHDPWPKPSYLFALVAGDLVA
                     RSDRFTTMSGREVALNIWVRPGDEDRTQHAMASLIASMKWDEEAFGREYDLDLFNIVA
                     VSDFNMGAMENKGLNVFNTRYVLADPATATDGDYDAVEGVIGHEYFHNWSGNRITCRD
                     WFQLSLKEGFTVLRDQLFSAAMGSEPVKRIEDVRILRAAQFPEDSGPLAHPIRPDSYQ
                     EISNFYTATVYNKGAEVIRMMRTMAGEQRFRAGTDLYFARHDGEAATCEDFITAIEEG
                     AGLDLKQFRLWYSQAGTPRVTARLSHEGDVATLHLAQVVPSTPGQDAKQPVPIPLRLA
                     LFDRATGKHSGEQLVVLDRAEAAFTFPGCTTRPVLSLNRGYSAPVAIEADQGAEDLVF
                     LAAHDDDPFARYEALQSLIVHHLAAAVAGRLDEASRLAGREAIGAALAAVLDDGALDD
                     LMRGELLVLPGEGYLAEQFAEADPTAIWQEREGLKAWLAQTLADRFRALHARASAVPY
                     SREAGARGARKVKTQALVYLAAGLPDEAASLAAAQYDAADNMTDRQGALMVLCGLETP
                     LRAEKLADFHARHAGNALVTDKWFSLQAGSLHPKALDHVKALADHADFTLANPNRVRS
                     LYMALAANPHAFHDPSGEGYRIIADLILALDPLNAQTAARFVAPLGRWRRIEPGRAAM
                     MRTELERIAAVPTLSRDTREQVMRSLG"
     gene            5147..>5684
                     /locus_tag="VN627_08815"
     CDS             5147..>5684
                     /locus_tag="VN627_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03395.1"
                     /translation="MRRMFVFGMGYSAGFIAAAAEARGIEVISTGRAGTLSFDDEGTV
                     RLALADADAVLSSVPPSGEGLDPVLERYGDALGGKWLGYLSSTGVYGDTGGAWVDETA
                     PVGTGRRTARSECDREWLARGARVFRLPGIYGPGRSALDRVREGRAHRIAPADSGPGQ
                     VFSRVHVADIASGVMAGLA"
BASE COUNT          924 a         1735 c         2145 g          880 t
ORIGIN      
        1 cgtcgccagc gggttggcgg gaatgtaatt gtccgggtgg ccgagatact tgggcgcgaa
       61 atagagcacc agcgcgaaca gcaagaggca aaccgccgcc ccgaagccgt ccttggcggt
      121 gtagaaagga tggaagggca gcgtgtcctt ctcgtccttg acgtcgaccc cggtcggatt
      181 gctcgagcct ggaatgtgca gcgcccagat gtgcaggatg accaccgcgg caatcacgaa
      241 gggcagcagg aagtgcagcg agaagaagcg gttcagcgcg gcctggtcgg gcgcgaagcc
      301 gccgagcagc caggtgtgga gcgcgtcgcc gaccagcggg atggccgaga acaggccggt
      361 gatgacctgg gcgccccaga agctcatctg gccccagggg agaacgtagc ccatgaaggc
      421 ggtggccatc atcagcagga agatcaccac gccgagcagc cagatcatct cgcgcggggc
      481 cttgtacgaa ccgtagaaga agccgcggaa gatgtggacg tagacgacga cgaagaaggc
      541 cgaggcgccg ttggcgtgcg catagcgcat catccagccc cagttcacat cgcgcatgat
      601 gtgctcgacg ctgtcgaacg ccacgccggc gttcgccgcg tagtgcatgg ccaggatcac
      661 gccggtgacg atctgcagga cgaggcagaa gcccgccagc acgccgaagt tccacgcgta
      721 gttcaggttg cgcgggacgg gatagccggc gccgatggcg ttgtagacga atcgcggcag
      781 gggcagcttt tcgtccagcc actgcatcag cggcgcttgc ggcttgtatt cgttggccca
      841 ggggaagctc atgacggtct acctcagccg atcttcacga cagtgtcgga ggtgaaagca
      901 tattccggaa cctgcaggtt cttcggcgcg gggcctttgc ggatgcgggc ggcggtgtcg
      961 tagaccgaac cgtggcaggg gcagaagtag ccgccgaatt cacccttcac ctcaccctcg
     1021 ccggcgccga gcggcacgca gccgaggtgg gtgcagacac ccatggtgat cagccagttt
     1081 tccttgcccg gcttggtccg ctccttgagg gtctgcggat cgcgcagggt ggaaatgtcg
     1141 acggcctcgg cctcggcgat ttccttgggc gtgagattgc gcacgaagac cggctgcttg
     1201 cggaacaccg ccttgatcgc ctggcccgag gcaatcgcac tgatatcgag ctcggtcgag
     1261 ctttcggcga gaacgtcggc cgagggggcc atctggctga tcagcgggag cagcgtgacg
     1321 acaccgccga cgcccgccgc gctgacggcg gcaatgttga tgaaatcgcg ccgacgcacc
     1381 ccttccccgt gttcggggag aacggcctca gtgcccgcat ggtctgacat ggtaaaccct
     1441 tgccctgcta taacaggccc gcaactggcg catttgcgcc gatcacgagc cccaatccgt
     1501 tgcggtcgtg caacccggag agcccccaaa acgcgcagac aagcggcgca attcagccgt
     1561 ccccggaacc gacttggcgc gctgatagac gcgaaaccat gccctgccaa ccgccttttt
     1621 gcggtgccgt gacagccttg gcaagcaggc ccggcgatca ggccccggat caggcgaggg
     1681 gcagggaaat ggcggaaatc tgccgcccat aggccgcttc gtggcggtgg gtcgaacggc
     1741 ggaaggagaa atagcgggct tcttgcgaat aggtatcaag accaagccgc tccaccgcgc
     1801 cgattccggc atggaccagc cgccgggcga gataggcagg cagatcgaac tgccagtgcg
     1861 cggcgcggcc gggggcgaag caggccgcgt ttgccggatc gtggccaagg aagcgatcgc
     1921 agaaggcgtc gtcgacctcg tagctggctt gcgcgatggt gggcccgatg gcggcggcga
     1981 tgcgatcggg ccgggcgcca agcgcgacca tggcggccac ggcggcgtcg gtcaccccga
     2041 cgagcgcgcc tttccagccg gcatgggcgg cgccgacgac ccccgcggtc cggtcggcaa
     2101 agagcacggg ggcgcaatcg gcggtgacca cgccgaggag gagcccgggg cggtcggtga
     2161 cgagggcatc ggcttcgggc cgggcatcgt cggcccaggg ctcggtcacg gtaacgcagg
     2221 tggccgaatg ggtctggtag accccgacca gccgcgcgcc gggggcaatg gcctgagcgg
     2281 cgcggcggcg gttttccgcg acggcggcct catcgtcgcc cgagccgagg ccgacgttga
     2341 gcgaggcggc aaccccggtg gagacaccgc cggtgcggcc gaggaagccg tgggggaagg
     2401 cggcgaggcc ttcggcgcgg gcgacgtcgg ccgtatcagc caaggctgcg catcacctgt
     2461 tcgcgggtat cgcgcgagag cgtgggcacg gcggcgatcc gctccagctc ggtgcgcatc
     2521 atggccgcgc ggcccggctc gatccgccgc caccggccca gcggggcaac gaagcgggcg
     2581 gcggtctgcg cgttcagggg atcgagtgcg aggatcaggt cggcgatgat gcggtaaccc
     2641 tcgcccgaag gatcgtggaa ggcgtgggga ttggccgcga gtgccatgta gagcgagcgg
     2701 acccggttgg ggttcgccag ggtgaagtcc gcatgatcgg cgagcgcctt gacgtgatcg
     2761 agcgccttgg ggtggagcga gccggcctga agcgagaacc acttgtccgt caccagcgca
     2821 ttgccggcgt ggcgagcgtg gaaatcggcg agcttttcgg cgcgcagcgg ggtttcgagg
     2881 ccacacagga ccatcagcgc gccctgccgg tcggtcatgt tgtcagccgc gtcgtattgc
     2941 gcggcggcga ggctcgcggc ctcgtccggg agaccggcgg cgaggtagac gagggcctgg
     3001 gtcttgacct tgcgcgcgcc gcgcgcgccg gcctcgcgcg aatagggcac ggcgctggcg
     3061 cgggcgtgga gggcgcggaa gcgatcggcg agggtctggg cgagccaggc cttgaggcct
     3121 tcgcgctcct gccagatcgc ggtcggatcg gcctcggcga actgttcggc gagatagcct
     3181 tcgccgggga ggaccagcag ctcgccgcgc atgagatcgt cgagcgcccc gtcgtcgagc
     3241 acggcggcca gcgccgcgcc gatggcctcg cgcccggcga ggcgggaggc ttcgtcgagc
     3301 cggccggcaa cggcggcggc gaggtggtgc acgatcaggc tttgcagcgc ctcgtagcgg
     3361 gcgaagggat cgtcatcatg ggcagcgagg aagacgagat cctcggcgcc ctgatcggcc
     3421 tcgatcgcga cgggagcgga atagccgcgg ttgagggaga ggaccggacg ggtggtgcag
     3481 ccggggaagg tgaaggccgc ctcggcgcgg tccagaacca ccagctgctc gccgctgtgc
     3541 ttgccggtgg ctcggtcgaa cagggcgagg cgcaagggga tcggcaccgg ctgcttggcg
     3601 tcctggccgg gcgttgacgg gaccacctgg gcgagatgga gcgtggcgac atcgccctca
     3661 tggctgagcc gcgcggtgac cctgggggtg ccggcctgcg aataccacaa gcggaactgc
     3721 ttgagatcga gcccggcgcc ctcctcgatc gccgtgatga aatcctcgca ggtggcggct
     3781 tcgccatcgt ggcgggcgaa atagagatcg gtgccggcgc ggaagcgctg ctcgcccgcc
     3841 atggtccgca tcatgcggat tacctcggcg cccttgttgt aaactgtcgc cgtatagaag
     3901 ttgctgattt cctgatagga atcggggcgg atcgggtgcg cgagcggacc cgaatcctcc
     3961 gggaactggg cggcgcgcag gatgcgcacg tcctcgatcc gcttgaccgg ttcggagccc
     4021 atggccgcgc tgaacagctg gtcgcgcagc accgtgaagc cttccttgag ggagagctgg
     4081 aaccagtcgc ggcaggtgat gcggttgccc gaccagttgt ggaaatattc atggccgatc
     4141 acgccttcga ccgcgtcgta gtcgccatcg gtggcggtgg cgggatcggc gagaacgtag
     4201 cgggtgttga agacgttgag gcccttgttc tccatcgcgc ccatgttgaa atccgacacg
     4261 gcgacgatgt tgaacaggtc gagatcgtat tcgcggccga aggcctcctc gtcccacttc
     4321 atcgaggcga tcaggctggc catggcgtgc tgggtacggt cctcgtcgcc cgggcggacc
     4381 cagatgttga gcgcgacctc gcggccggac atggtggtga agcggtcgct ccgggcgacg
     4441 agatcgccgg cgacgagggc gaagaggtag gacggcttgg gccagggatc gtgccattcg
     4501 gcccagtggg tgccgctttc gccttcgccc ttcgcgacgc agttgccgtt ggagagaagg
     4561 atcgggaact gcgccttgtc cccgctcatc cgcacccggt agaccgagag cacgtcgggc
     4621 cggtcgggat ggaaggtgat gcggcggaac ccttcggcct cgcactgggt gcagagcatg
     4681 ccgttcgagg catagaggcc catcaggtgc gaattggcgg ccggattgat ggcggtttcg
     4741 accgagactt cgtgggcatc gccttcgaga tcgatcagca ggtcgggccc gtccatgcgc
     4801 caggcattgc tctcgcgccc gtcgatccgg accgcgcgcg cggcgatccc gtcaccggcg
     4861 aggcgcaggg gggctgcctg aacggcgggg ttgcggcgga ccgagaggcg gctggtgacc
     4921 tgcgtggcgt cgagcccgag ggtgaaatcg agcgcgacct ccgggatcag ccaggccgga
     4981 gcgacatagt cttcccggcg gatcaccgga ggatgggccg gcggcggcgc ggcgtcggcg
     5041 gtttgcgggt tttcggcggg ggcggacggt ctgctggcaa tatccatggc attcttctta
     5101 gtgactgcgc ttccgattgc cagcaggcgc gctatgggaa tcggcgatgc gacgcatgtt
     5161 tgtcttcgga atgggctatt cggccgggtt catcgccgcg gcggccgagg cgcggggaat
     5221 cgaggtgatc tcgaccgggc gggccggcac gctgtcgttc gacgacgagg gcacggtgcg
     5281 gctggcgctg gccgatgccg atgcggtgct ctcctcggtg ccgccctcgg gggaggggct
     5341 cgatcccgtg ctcgaacgct atggtgacgc actggggggc aagtggctgg gctatctctc
     5401 ctccaccggg gtctatggcg atacgggtgg ggcctgggtc gacgagacgg cgccggttgg
     5461 caccggacgg cgaaccgccc gcagtgaatg cgaccgggaa tggctggcgc ggggcgcgcg
     5521 ggtgtttcgc cttccaggga tctatggccc ggggcgctcg gcgctggacc gggtgcgcga
     5581 ggggcgggcc caccggatcg cgcctgctga ttccgggccg gggcaggtgt tcagccgggt
     5641 tcacgtggcc gacatcgcca gcggggtcat ggccgggctg gcgg
//
LOCUS       DATNBV010000367         3442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2134784,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000367 DATNBV010000000
VERSION     DATNBV010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134784"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VN627_08820"
     CDS             complement(<1..1164)
                     /locus_tag="VN627_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03396.1"
                     /translation="MTTTTAKSATPAAIPWWTPGDWNAFFGFGTNILVNVLVLTGLLR
                     FVLKLPDPIIFGRILPAVGLMLFLSTVYYAWLARKLAVSTGRTDVCALPSGISVPHMF
                     VVVFVVMLPILIKSGDPLEAWGAGLTWVFVQSFVLMAGGFIAPVIRRITPRAALLGSL
                     AGISLTFISLSPGAQLFMTPVIGVVCLAVILASWLGNYRYPGGIPGGLMAIIVGSAIA
                     WGGQLLGFDIGGVGSDKVAGAVSDFGFHVPLPAVENIMGGFKYLGVILVTAIPFGIYD
                     LVEAMDNVESAEAAGDHFPTTRVLTADGIISLIGCCMGNPFINAVYIGHPGWKAMGGR
                     TGYSAATGALMLFLTWFGVVGLMMAIIPSVALLPILLYIGMLIGSQAFQESPHS"
     gene            complement(1182..1874)
                     /locus_tag="VN627_08825"
     CDS             complement(1182..1874)
                     /locus_tag="VN627_08825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HXD03397.1"
                     /translation="MAHVTARPYAFDLDPSRAALLIIDMQRDFVEPGGFGEMLGNDVA
                     QLRPSIAPLQTLLAAARAAGLFVIHTREGHKPDLSDLHEAKRLRGRGATTIGDPGPMG
                     RILIIGEPGHDIIPELYPVPGEPVIDKPGKGAFYNTELADIIAERGIAQLIVCGVTTE
                     VCVHTTVREANDRGINCLVLSDCTASYFPEFHEAALRMVAAQGGIFGWVSDSANVIAA
                     LGMTEPAEPAAN"
     gene            complement(1929..2783)
                     /locus_tag="VN627_08830"
     CDS             complement(1929..2783)
                     /locus_tag="VN627_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03398.1"
                     /translation="MRKMLIGLSSLATLGWAGAAHAEDGSWELHGFGDVSVKSDYVTP
                     RGLVVVDDGVTVQVLDGLVLKSPGGVTFHAGTWTDLNPGYNHAENITAVNEFDFFVGV
                     GAYITPKVEVGAEYSQFVSGQPSVAFKNEHNIEFTVKYHDGGKDSPITINPYAKLFWA
                     VDSKSSTVVLGKAGGTFDVELGAVPTIKAGPAKISMPTWITVGPKTYWGNTSDGISDS
                     NFGVVSTGLKAETPLTFLKGGAGASVYAQVQYYHLINDNLTVARTLLTNSTGRDKVVF
                     GAGLSIGF"
     gene            2993..>3442
                     /locus_tag="VN627_08835"
     CDS             2993..>3442
                     /locus_tag="VN627_08835"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXD03399.1"
                     /translation="MSGTTAEKLAENLAQAIIDGDFAPGTRLDEQQLAQRFAVSRTPV
                     REAIRQLASSGLIEVRPRRGAFVAEISPAQLEILFVAMGELEASCARLAALSMSPPER
                     RRLEAFHEQMGQIARGSDASAFAEANHAFHTMLYAGTHNPVLAEMTAA"
BASE COUNT          612 a         1069 c         1165 g          596 t
ORIGIN      
        1 gctgtgcggg ctctcctgga aggcctggga gccgatcagc atgccgatgt agagcaggat
       61 cggcagcagg gcgaccgagg ggatgatcgc catcatcagg ccgaccacac cgaaccaggt
      121 gaggaacagc atcagcgcgc cggtcgcggc cgaatagccg gtgcgcccgc ccatcgcctt
      181 ccagccgggg tggccgatgt agacggcgtt gatgaagggg ttgcccatgc agcagccgat
      241 gaggctgatg atgccgtcgg cggtcaggac gcgcgtggtc gggaagtgat cgcccgccgc
      301 ttcggcgctc tcgacattgt ccattgcttc gacgaggtcg tagatgccga agggaatggc
      361 ggtgacgagg attacgccca gatacttgaa gccgcccatg atgttctcga ccgcgggcag
      421 cggcacgtgg aacccgaaat cggacacggc tccggcgacc ttgtcactgc cgacaccgcc
      481 gatatcgaag cccagcaact ggccgcccca ggcaatggcc gagccgacga tgatcgccat
      541 gaggccgccg ggtatgccgc cgggatagcg gtagttgcca agccatgagg cgaggatgac
      601 ggcgaggcag accacgccga tgaccggcgt catgaacagc tgcgcgcctg ggctcagcga
      661 gatgaaggtg agcgagatgc ctgccagcga gccgaggagg gcggcgcgcg gcgtgatccg
      721 gcggatcacc ggggcgatga agccgccggc catgagcacg aagctctgca cgaagaccca
      781 ggtcaggccc gcgccccatg cctccagcgg atcgccggac ttgatcagga tcggtagcat
      841 cacgacgaag acgacgacga acatgtgcgg cacgctgatg ccggacggca gggcgcagac
      901 atcggtgcgg ccggtgctga ccgccagctt gcgggcgagc caggcgtaat agaccgtgct
      961 caggaacagc atcaggccca cggcgggcag gatgcggccg aagatgatcg gatccggcag
     1021 tttcagcacg aaccggagca ggccggtgag gaccagcaca ttgaccagga tgttggtgcc
     1081 gaagccgaag aaggcgttcc agtcgccggg cgtccaccag gggatcgccg ctggcgtggc
     1141 tgatttcgcg gtcgttgtgg tcatgctcgt acccccacgt gctagttggc tgccggttcc
     1201 gccggttcgg tcataccaag tgccgcaatc acgttcgccg agtccgagac ccagccgaag
     1261 atgcccccct gcgctgcgac catgcgcagg gcggcctcat ggaattcggg gaaataggag
     1321 gcggtgcagt ccgacaggac gagacagttg atgccccggt cgttcgcttc gcgtaccgta
     1381 gtgtggacgc agacttcggt cgtcaccccg cacacgatga gctgggcgat cccgcgctcg
     1441 gcgatgatgt cggcgagttc cgtgttgtag aaggcgccct tgccgggctt gtcgatcacc
     1501 ggctcgcccg gcaccggata aagctcggga atgatgtcgt ggccgggctc gccgatgatc
     1561 aggatccgcc ccatgggccc gggatcgccg atggtcgtgg ctccgcggcc gcgcaagcgc
     1621 ttggcttcgt gcaggtcgga caggtcgggc ttgtggcctt cgcgcgtgtg gatgacgaac
     1681 aggcccgcag cccgcgccgc ggccagcagg gtctggagcg gggcaatcga cgggcgcagc
     1741 tgcgccacgt cgttgcccag catctcgccg aacccccccg gttcgacgaa atcgcgctgc
     1801 atgtcgatga tgagcagggc cgcccggctg gggtccaggt cgaacgcgta gggtcgtgca
     1861 gtcacgtgtg ccatgacagt cgctccatgg ggttccgcca gttcagccga gcggcccgtg
     1921 ccggtggatc agaagccgat gctcaggccc gcgccgaaca cgaccttgtc gcgcccggtc
     1981 gaattggtga ggagggtccg ggccacggtc aggttatcgt tgatgaggtg gtaatactgg
     2041 acctgcgcgt agaccgaggc gcccgcgccg cccttgagga aggtcagcgg ggtctcggcc
     2101 ttgagaccgg tcgagaccac gccgaaattg gaatcgctga tcccgtcgct ggtgttgccc
     2161 cagtaggtct tgggcccgac cgtgatccag gtcggcatgg agatcttggc cggccccgcc
     2221 ttgatcgtcg gcaccgctcc aagctcgacg tcgaaggtac cgccggcctt gcccagcacg
     2281 acggtggacg acttgctgtc cactgcccag aacagcttgg catagggatt gatggtgatc
     2341 ggcgaatcct tgccgccatc gtggtacttc acggtgaatt cgatgttgtg ttcattcttg
     2401 aatgccaccg acggctggcc ggagacgaac tgggagtatt cggcccccac ttcgaccttc
     2461 ggggtgatgt aggcacccac gccgacaaag aaatcgaact cgttgaccgc cgtgatgttt
     2521 tcggcgtggt tgtagcccgg attgagatcg gtccacgtac cggcatggaa ggtcacaccg
     2581 cccggcgact tgaggacgag cccgtccagc acctgcacgg tcaccccgtc atccacgacg
     2641 acgagaccgc gcggcgtgac atagtcgctc ttgaccgaga cgtcaccgaa gccgtgcaat
     2701 tcccagctgc cgtcttcggc atgggcagca cctgcccagc ccagagtggc gaggctcgaa
     2761 agtccgataa gcattttacg catgttgggt cgctccctgt ttggttggcg cccaagcatc
     2821 ccccgcggct ccgtgcagaa cacggcccac ccgtctttgt atgcgttatg tcacttatgc
     2881 atacaattta tgtgttcgca atggtgttgt ggtatctgat gctcctgcct atttctggcc
     2941 tcgacaaagc cctgtcccga cgtgggcgct ggtgtgtcgc ggagtgtgga tggtgagcgg
     3001 cacgactgcc gaaaagctgg cggaaaacct ggcccaggcc atcattgatg gtgattttgc
     3061 ccccggcacc cggctcgacg aacagcagct cgcgcagcgt ttcgcggtct cgcgcactcc
     3121 ggtgcgcgag gctattcgcc aactggcgag cagcgggctg atcgaggtgc ggccccgtcg
     3181 gggcgccttc gttgccgaga tatcgccggc tcagctcgag atactgttcg ttgccatggg
     3241 ggagctggaa gcgagctgcg cccgccttgc ggccctgtcg atgtctccac cggagcgccg
     3301 ccggcttgaa gcgttccacg aacagatggg gcagatcgcg cggggcagcg atgcctcggc
     3361 tttcgccgag gcgaaccatg cctttcacac aatgctctac gccgggacgc acaatcccgt
     3421 gctcgcggaa atgaccgccg cg
//
LOCUS       DATNBV010000368         3368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2422252,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000368 DATNBV010000000
VERSION     DATNBV010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2422252"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..614)
                     /locus_tag="VN627_08840"
     CDS             complement(63..614)
                     /locus_tag="VN627_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXD03400.1"
                     /translation="MRPRQTLPNLWLVSDARTDARDESGLERAMARLPRGAGVVFRHY
                     HLPEREREARFRSLARIARGRGLWLAWAGSAREARALGADACYGPAALLTRGPALGRL
                     VTAHALREIGMAERARADAVLLSPVFPTRSHPGGKVLGALRFRLLAARSRLPVLALGG
                     MNARRARALSGFGWAAIDGLAWL"
     gene            637..1299
                     /locus_tag="VN627_08845"
     CDS             637..1299
                     /locus_tag="VN627_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HXD03401.1"
                     /translation="MEQPATLLQTVRDRIAHAAAIARRKAGEITLVAVSKTHGPEAIA
                     PLIAAGQRVFGENRVQEAQAKWPALRDAHGAIELHLVGQLQSNKADDAVALLDCIHSV
                     DRPSLVTALARAMDKTGRRLPCFLQVNIGAEEQKGGCAIADLPALLAEARAADLPVTG
                     LTCVPPAGIEPAPFFALLDKLAADHGLPGRSMGMSDDFETAIMLGATHVRVGSALFGA
                     RE"
     gene            complement(1418..1705)
                     /locus_tag="VN627_08850"
     CDS             complement(1418..1705)
                     /locus_tag="VN627_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXD03402.1"
                     /translation="MGRGGFTYIMTNRAYGVLYVGVTADIHQRIAQHRGGLGSRFCRR
                     YGLDRLVLVEPHESIEQAIAREKQLKNWRREWKLELVETANPDWADLAGLF"
     gene            complement(1895..3349)
                     /gene="pyk"
                     /locus_tag="VN627_08855"
     CDS             complement(1895..3349)
                     /gene="pyk"
                     /locus_tag="VN627_08855"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HXD03403.1"
                     /translation="MTGSKTGYRGRRVKILATVGPASRSPEMIARLLRAGVDAFRVNM
                     SHGDHATHAETIATIRAAEKDFGRPVAILCDLQGPKLRVGTFANGRERIRHGAHFTLD
                     RDPAPGTEERVCLPHPELFGVLKAGQRLLIDDGKVQLRVIKAGADAILTTAEVGGFIS
                     DRKGVNVPDAVVPVPALTEKDRRDLAFAIEHHADWIALSFVQRPEDVAEARKLMGGYG
                     ALVAKIEKPAAVDRLEEIIELSDGIMVARGDLGVELNPEEVPPLQKRIVEATRRSGKP
                     VIVATQMLESMIESPAPTRAEVSDVANAVYDGADAVMLSAETASGAWPVEAVTIMDRI
                     GKQVEADPGYAARVHFTDVRPDATTADALAQACANIAETVTLAGIIVFTGSGSTARRV
                     ARERPAVPMLVLTPSPRTARRTALLWGAHAVHTREIGSFEEMIAKGKRMALRHGFGAA
                     GSKLVTLAGVPFGVPGSTNLLHVVTLTGQELDGE"
BASE COUNT          542 a         1321 c         1035 g          470 t
ORIGIN      
        1 gaggggccgg gggtgggcaa gccaacaagc gcagctccct atagccccac gtcccaacaa
       61 gcttagagcc acgccaaccc gtcgatcgcc gcccagccaa acccgctcag ggcccgcgcg
      121 cgccgcgcgt tcatgccgcc cagcgccagc accggcagcc ggctgcgcgc cgccagcagt
      181 cggaaccgca gcgcccccag caccttgccg ccgggatggg accgcgtcgg aaaaaccggc
      241 gaaagcagca ccgcatcggc ccgcgcccgc tcggccatgc cgatctcgcg cagcgcatgc
      301 gcggtcacca gccgtcccag cgccggtccc cgcgtcagca aggcagcggg gccgtagcag
      361 gcgtcagccc ccagcgcccg cgcctccctc gccgaaccgg cccaggccag ccacagccct
      421 cgcccacgcg cgatccgcgc cagcgagcga aaccgcgcct cccgctcccg ctcaggcaag
      481 tgataatgcc ggaacaccac cccggccccg cgcggcagcc gcgccatggc gcgctccagc
      541 ccggattcgt cccgggcatc ggtgcgggca tcggaaacca gccacaggtt cggcagggtc
      601 tggcgcgggc gcatccgaac gctatagcgc agcagaatgg aacagccagc gacccttctc
      661 cagaccgtcc gcgaccgcat cgcccacgcc gccgcgatcg cgcgccgcaa ggccggcgaa
      721 atcaccctcg tcgccgtcag caagacgcac gggcccgaag ccatcgcccc gctgatcgcg
      781 gccggccagc gggttttcgg cgaaaaccgg gtgcaggaag cgcaggccaa gtggccggcc
      841 ctgcgcgacg cgcatggcgc gatcgaactc cacctcgtcg gccagctcca gtccaacaag
      901 gccgatgacg ccgtcgccct gttagactgc atccactcgg tcgatcgccc ctcgctcgtg
      961 accgccctcg ccagggccat ggacaagacc ggccgccgcc tgccgtgctt cctccaggtc
     1021 aacattggcg ccgaagagca gaaaggcggc tgcgcgattg ccgacctgcc ggccctgctg
     1081 gccgaggccc gcgccgccga cctcccggtg accggcctga cgtgcgtccc ccccgccggc
     1141 attgaaccgg ccccgttctt cgccctgctc gacaagctcg ccgccgatca cggcctcccc
     1201 ggccgctcga tgggcatgag cgacgatttc gaaaccgcga tcatgctcgg cgccacccac
     1261 gtgcgggtag gttcggccct gttcggcgcg cgggaataac gccgtcccgg gctcgacccg
     1321 ggaccgctgg ccaacgcaaa gcggagagtc ctcctgcagg caagcccaca gctccgcccc
     1381 cgcaccggtc ccgcgtcaag cccgggacga cgccggatta gaacagcccc gccagatccg
     1441 cccaatccgg attggccgtc tcgaccaatt ccagcttcca ctcccgccgc cagttcttca
     1501 gttgcttttc ccgggcgatg gcctgctcga tagattcgtg cggctcgaca agcaccaacc
     1561 ggtccaatcc atacctccgg caaaaccgtg agcccaagcc gccgcgatgc tgcgcaatcc
     1621 gctgatgaat gtcagccgtc acgccgacat aaagcacccc gtaggcgcgg ttcgtcatga
     1681 tgtaagtgaa cccgcccctg cccataaccc gaacatgccc ggccacgttg agcggtcaag
     1741 cctaacgtcg tcccgggctc gacccgggac cgctggccaa cgcgaagccg agagcttgcc
     1801 tgctcgagag gtcacagctc cgccaccccc tccggtcccg ggtcaagccc gggacgacgc
     1861 caagcccggg acgacgccta gcccgggacc atgcctactc cccgtccagc tcctgcccgg
     1921 tcagcgtcac gacatgcagc aggttggtcg accccggcac gccgaacggc acgcccgcaa
     1981 gcgtcaccag cttcgatccc gccgctccga agccatggcg cagggccatg cgcttgccct
     2041 tggcgatcat ctcctcgaac gagccgatct cgcgggtatg gaccgcatgg gcgccccaca
     2101 gcagcgccgt gcgccgcgcc gtccgcgggc ttggcgtgag caccagcatc ggcacggctg
     2161 gccgctcccg ggccacgcgg cgcgcggtcg aacccgagcc ggtgaagacg atgatccccg
     2221 ccagcgtcac cgtctcggcg atattggcgc acgcctgggc cagcgcatcg gcggtggtcg
     2281 catcggggcg cacgtcggtg aagtggacgc gggcggcata gccgggatcg gcctccacct
     2341 gcttgccgat gcggtccatg atggtcacgg cttccaccgg ccatgccccg ctcgccgtct
     2401 cggccgaaag catcaccgca tcggccccgt cgtaaaccgc attggccacg tccgacactt
     2461 cggcacgggt cggcgccggg ctttcgatca tcgattcgag catctgggtc gccacgatca
     2521 ccggcttgcc cgaacggcgc gtcgcctcga cgatgcgctt ttgcagcggc ggcacttcct
     2581 ccgggttgag ctcgacgccc aggtcgccgc gggcgaccat gattccgtcc gatagctcga
     2641 tgatctcctc cagccggtcc accgccgccg gcttctctat cttggccacc agggccccgt
     2701 agccgcccat cagcttgcgc gcctcggcca cgtcctcggg ccgctggacg aacgagagcg
     2761 cgatccagtc ggcatgatgc tcgatcgcga aggcaaggtc gcggcggtcc ttctcggtca
     2821 gcgccggcac cggcaccacc gcatcgggca cgttgacacc cttgcggtcg gagatgaacc
     2881 cgccgacctc ggccgtggtc aggatcgcat cggccccggc cttgatcacc cgcagctgga
     2941 ccttgccgtc gtcgatcagc aggcgctgcc cggccttcag cacgccgaac agctccgggt
     3001 gaggcaggca gacgcgctcc tctgtgccgg gtgccggatc gcggtcgagc gtgaaatgag
     3061 cgccgtggcg gatccgctcg cgcccgttgg caaaagtgcc cacgcgcagc ttggggccct
     3121 gcaggtcgca caggatcgcc accggccgcc cgaaatcctt ctccgccgcc cggatcgttg
     3181 cgatggtctc ggcatgcgtg gcatggtcgc catggctcat attgacgcgg aacgcgtcga
     3241 ccccggcccg cagcaaccgc gcgatcatct ccgggctccg gctggccggg ccgacggtgg
     3301 ccagaatctt gaccctccgc ccgcgatatc ctgtcttcga accggtcacg ttttatcctt
     3361 ccgctgcg
//
LOCUS       DATNBV010000369         2593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_230611,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000369 DATNBV010000000
VERSION     DATNBV010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230611"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1646
                     /locus_tag="VN627_08860"
     CDS             <1..1646
                     /locus_tag="VN627_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXD03404.1"
                     /translation="PFETVLAADTERAALMAESEDCHDGHRLAEIHERLIAIEAHSAP
                     ARAARILVGLGFDEDAQHRPLESFSGGWRMRVALAALLFSQPDLLLLDEPSNHLDLEA
                     VLWLEDFLRSYPATILLVSHERDFLNNVVDHILHLSQGKLTLYPGGYDAFERQRAERQ
                     AQIASARARQQAERERLRDYVARNSARASTAKQAQSRAKALAKLQPIAELIDDPSLTF
                     DFPNPDELRPPLITLDLASVGYGEVPVLQRLNLRLDPDDRIALLGRNGNGKTTLARLL
                     AAQLAPMAGSMAASGKMKVGYFTQYQVEELDRTETPLQHMTVLMKGATPAAVRAQLGR
                     FGFAGPKAETQVGKLSGGERARLALALITRDAPHMLILDEPTNHLDVDAREALIQALN
                     AYSGAVVIVSHDRHMLEMTADRLVLVDGGTAREFDGAIDDYIAFVLAKDPAPARRDDG
                     TQRGVNKKDQRRANAEARERGQELRKRAKSAEAELEKLTAQRNAVDRAMFDPASAEPA
                     LAKLTMTDLMKRRADLEAKIETAEAAWLQASEALEAIAA"
     gene            1643..2104
                     /locus_tag="VN627_08865"
     CDS             1643..2104
                     /locus_tag="VN627_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HXD03405.1"
                     /translation="MSVAFRRDGDDEHLEPKFEIPIPPGPNLVTARGKALIDEKVTAL
                     ETAIASAEPQRVEALKRELRYWQTRQVTAEVVPAPSGDTVEIGTTVSLRLNGKARVLS
                     IVGDDEGDPGAGLVAFSSPLARALMGAEVGEMVDWAGTEGAIEVQAITAAA"
     gene            2318..>2593
                     /locus_tag="VN627_08870"
     CDS             2318..>2593
                     /locus_tag="VN627_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXD03406.1"
                     /translation="MNFMNLFQFSAADLAIDLGTANTVVYLREQGIVVDEPSAVAIET
                     NNGIPRVRAVGDDAKLMLGKTPDNVRTIRPLRNGVVADLEVAEQMIKH"
BASE COUNT          454 a          909 c          858 g          372 t
ORIGIN      
        1 ccccgttcga aacggtgctg gccgccgaca ccgaacgtgc cgcgctcatg gccgagagcg
       61 aagattgcca cgacgggcac aggctggccg agattcatga acggctgatc gctatcgagg
      121 cccattccgc cccggcgcgg gccgcgcgca tcctggttgg cctcggcttc gacgaagacg
      181 cgcaacaccg gccgctcgag agcttttccg gcggctggcg aatgcgggtg gcgctggcgg
      241 ccctgttgtt ttcccagccc gacctcctgc tgctcgatga accttccaac caccttgatc
      301 tcgaggccgt gctgtggctg gaggatttcc tgcgctccta cccggcgacc atcctgctgg
      361 tcagccatga gcgcgatttc ctcaacaacg tggtcgatca catcctgcac ctctcgcagg
      421 gcaagctgac gctttatccg gggggctacg acgccttcga gcgccagcgc gccgagcggc
      481 aggcgcagat cgcctcggcc cgggccaggc agcaggccga gcgcgagcga ctgcgcgact
      541 atgtcgcccg caattctgcc cgcgcatcca ccgccaagca ggcccagtcg cgcgccaagg
      601 ccctggccaa gctccagccc atcgccgagc tgatcgacga tccgtcgctc accttcgatt
      661 tcccgaaccc cgacgagctg cgcccgccgc tgatcacgct cgaccttgcc tcggtcggct
      721 atggcgaggt gccggtgctg cagcggctga accttcggct cgaccccgac gaccgcattg
      781 ccctcctcgg ccgcaacggc aacggcaaga ccaccctcgc ccgcctgctc gccgcccagc
      841 tcgcgcccat ggccggcagc atggcggcca gcgggaagat gaaggtcggc tatttcacgc
      901 agtaccaggt cgaagagctc gaccggaccg agaccccgct ccagcacatg accgtgctca
      961 tgaagggggc gacccccgcg gcggtgcggg cgcagctcgg ccggttcggc tttgccgggc
     1021 cgaaggcgga aacgcaggtc ggcaagcttt cgggcggcga gcgggcgcgg ctggcgctgg
     1081 cactgatcac gcgcgacgcg ccgcacatgc tgatcctcga cgagccgacc aaccacctcg
     1141 acgtcgatgc gcgcgaggcg ctgatccagg cgctcaacgc ctattcgggc gccgtggtga
     1201 tcgtcagcca cgaccgccac atgctggaga tgaccgccga ccggctggtg ctggtggacg
     1261 gcggcacggc cagggagttt gatggggcga tcgacgacta cattgccttc gtcctcgcca
     1321 aggacccggc gccggcacgg cgagacgacg ggacccagcg cggcgtcaac aagaaggacc
     1381 agcgccgtgc caacgccgag gcgcgcgaac gcgggcagga actgcgcaag cgggccaaaa
     1441 gcgcggaggc cgagctcgag aagctcaccg cacaacgcaa cgcggtcgac cgggcgatgt
     1501 tcgatcccgc cagcgcggag ccagcattgg cgaagctgac catgaccgac ctgatgaagc
     1561 gccgcgccga cctcgaagcg aagatcgaaa ccgccgaggc cgcctggctg caggccagtg
     1621 aggcgctgga agcgatcgcc gcatgagcgt cgccttccgc cgcgatggcg atgacgagca
     1681 cctcgagccc aagttcgaga tccccattcc gcccggcccg aacctggtca ccgcgcgcgg
     1741 caaggcgctg atcgatgaga aggtcaccgc gctggagacc gcaatcgcct ccgccgaacc
     1801 ccagcgggtg gaggcgttga agcgggaact gcgctactgg caaacccggc aggtgaccgc
     1861 cgaggtggtg ccggcgccct cgggggacac ggtggagatc ggcacgaccg tcagcttgcg
     1921 gctgaacggc aaggcccggg tattgtccat cgtgggcgat gacgagggcg atcccggcgc
     1981 cgggctggtg gccttctcct ctcccctcgc ccgcgcgctg atgggcgcag aggtaggcga
     2041 gatggtcgac tgggcaggga cggaaggcgc aatcgaggtt caggccatta cggcagccgc
     2101 ctgacgtcgc cccggacttg atccgggacc gctggcagac ttcaggcaga acctttcctg
     2161 tcggcgcgat ggacaccggc cacgggtctg tcccgcgacg acggaacgcc gaaatcgccc
     2221 tgccgcgctg cccactcact tcgcggcctc gttcatgtgc gcgacagctt gccccccctc
     2281 tagccctccc gacctgtcgg gaggggcgct taacgcaatg aacttcatga atttatttca
     2341 gttctccgcc gccgacctgg ccattgacct ggggacggcg aacacggtgg tgtacctgcg
     2401 cgaacagggg atcgtggtcg acgagccatc ggcagtggcg atcgagacca acaacggcat
     2461 tccccgcgtc cgcgccgtgg gcgacgatgc caagctgatg ctgggcaaga ccccggacaa
     2521 cgtgcggacc atccgcccgc tgcgcaacgg ggtcgtggcc gatctcgaag tcgcagaaca
     2581 gatgatcaag cac
//
LOCUS       DATNBV010000370         2194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2773512,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000370 DATNBV010000000
VERSION     DATNBV010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2773512"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VN627_08875"
     CDS             <1..998
                     /locus_tag="VN627_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HXD03407.1"
                     /translation="AAAERHLPQLLAERATALTAGIAGGTGGLALVDGEVRRGGEVLA
                     RLKPGKGLFAPQLQLDNALEAIPAAERRALREALEQWLAAWLALAAPLAALDSASREA
                     EGGPELRAMLIRMVDGGGVIVREGSGLDQLQPVQRDALRKLGVRIGAIDLFVPSALKP
                     GPLGVWRELAQQRGLRTGDVLDQMPPVAALAKGRAPLGYRRIGQQAIRIDMAEKLLRE
                     AHGRRVGAGKAMFTVDPALARSMGFSLDGHVQLLRQAGFLAYSPTELPEGAYGPPAPI
                     NWRWRPPRPRDPAVTKDRHHGRNQGHSGRPAKAVPEPKAAPAATGAFAALAALKL"
     gene            1010..1336
                     /locus_tag="VN627_08880"
     CDS             1010..1336
                     /locus_tag="VN627_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HXD03408.1"
                     /translation="MRLDKLLWFLRFTKTRPLAQALAEAGHIRLNGRRVERSAQGVQV
                     GDVLVLPLSAGVRVVEIVALPDRRGPASEAEACYRALDAGAEISIAAGSNRTGSRPDH
                     QGDLLQ"
     gene            1333..1671
                     /gene="fdxA"
                     /locus_tag="VN627_08885"
     CDS             1333..1671
                     /gene="fdxA"
                     /locus_tag="VN627_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008528408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HXD03409.1"
                     /translation="MTYVVTDACIRCKYMDCVEVCPVDCFYEGENMLVIHPDECIDCG
                     VCEPECPAEAIKPDTEPGLEKWLGLNAEYAKIWPNITVKKDGPPDGKDWEGVPDKFEK
                     HFSPKPGQGD"
BASE COUNT          401 a          723 c          738 g          332 t
ORIGIN      
        1 ttgccgcggc cgagcgccac ctgccgcaac tgctggccga gcgggcgacc gcccttacgg
       61 cgggaatcgc gggcggaacc ggcggattgg cactcgtgga cggagaagtc cggcgcggcg
      121 gcgaagtgct ggcgcggctc aagcccggca agggcctgtt cgcgccccag ctccagctgg
      181 acaatgcgct cgaggcgatc cccgccgccg aacggcgcgc gctgcgcgaa gcactggagc
      241 agtggttggc ggcctggctg gccctcgccg cgccgctggc cgcgctcgac agcgcgagcc
      301 gcgaagcgga aggcgggcca gaactgcgcg ccatgttgat caggatggtc gacggcggcg
      361 gcgtgatcgt gcgcgaaggc tcggggctgg accagctcca gcctgtccag cgcgatgcgc
      421 tgcgcaagct gggagtgcgg atcggagcga tcgacctgtt cgtgccaagc gccctcaagc
      481 cgggcccgct cggcgtgtgg cgcgaactgg cgcagcagcg ggggctgagg accggagacg
      541 tgctcgacca gatgccgcct gtcgctgccc tggccaaggg ccgggcgccg ctcggctatc
      601 gccgcatcgg ccagcaggcc atccgcatcg acatggcgga aaagctgctg cgcgaggccc
      661 atggccgccg ggtcggggcg ggcaaggcga tgttcaccgt cgaccccgcg ctggcccgct
      721 cgatggggtt ctcgctcgac ggccatgtcc agctcctgcg gcaagccggg ttcctggcct
      781 attcgccgac cgaactcccc gaaggagcct atggaccgcc cgcgccgatc aattggcgct
      841 ggcgtccccc ccgcccccgc gatcctgcgg tcaccaagga ccgccatcac ggtcgcaatc
      901 aggggcactc tggccgaccg gccaaggctg tgccggagcc caaagccgcg cccgcggcga
      961 ccggggcatt cgcggcactg gctgccttga aactctaggc cggcagcgcg tgcggctcga
     1021 caagctcttg tggttcctgc gcttcaccaa gacgaggccg ctggcccagg ccctggccga
     1081 ggccgggcac atccggctca acggccgacg ggtggagcgc agcgcccagg gggtgcaagt
     1141 gggcgacgtg ctggtcctgc cgctgtcggc gggcgtgcgg gtcgtcgaaa ttgtcgccct
     1201 gcccgaccgg cgcggccccg ccagcgaggc cgaggcctgc taccgtgcgc ttgacgcggg
     1261 cgcagaaatt tccatagcag cgggatcgaa tcggaccggg tcgcgcccgg atcaccaagg
     1321 ggacctattg caatgaccta tgtcgtcact gacgcctgca tccgctgcaa gtacatggac
     1381 tgcgtcgagg tctgcccggt tgactgcttc tatgaaggcg agaacatgct cgtcattcat
     1441 ccggacgaat gcatcgattg cggcgtctgt gaaccggagt gcccggccga ggcgatcaag
     1501 cccgacaccg agcccggact ggaaaagtgg ctgggcctca acgccgaata cgcgaaaatc
     1561 tggccgaata ttacggtcaa gaaagacggc cctcccgacg gtaaggattg ggaaggcgtt
     1621 ccggacaagt tcgagaagca cttctcgccg aaacccggac agggtgactg acccttttcc
     1681 gggtgccccc aagtccgggc tcagctgccg agacgtcgtt aaccaatttt aacggcattg
     1741 ccgccgtttg gcgcgaaaac tgcctttttg cagtgcaata acgctttgat tctgaccata
     1801 aatgtgttat acgcatgaca tgatggattt tcgcccgagg ctccccgtgg ggagcattat
     1861 ttttcgggac caggataccc acataaaccc gtgaaggggc gtgcagagag cacgcataag
     1921 cttgtggcag ggaagatgcg tcagaaatcc aagaagggtt cgtccggctc gagccggcgc
     1981 accaaaaaac catccgctgg cgccgtccgg cggacatcgc aggctacacg cggcgcaacc
     2041 gcccgacgtg cagcccccgt gaccaaggca gccggccgca agccggaaca aggagcaatt
     2101 cctgtaatga ccgccaagaa gaccacccaa cggcagggct tcaagaccaa cgaatatatc
     2161 gtctatccgg cgcacggcgt cggccagatc atgg
//
LOCUS       DATNBV010000371         6065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3711258,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000371 DATNBV010000000
VERSION     DATNBV010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3711258"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VN627_08890"
     CDS             complement(<1..179)
                     /locus_tag="VN627_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HXD03410.1"
                     /translation="MPLLEARNTYKPFEYPWAYEFWKRQQQVHWMPEEVPLGEDCRDW
                     AQKISDHERNLLTQI"
     gene            complement(191..925)
                     /locus_tag="VN627_08895"
     CDS             complement(191..925)
                     /locus_tag="VN627_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXD03411.1"
                     /translation="MTAPLILWGMPSPNVVKVVLMIEECALECELRHVGVFTGEQFAP
                     GFRAKSPLAKVPVLEDPRLGRPLVESGAILLWLAEREGRFLPAAQPARAEVMQWLMIQ
                     MANVGPMFGQVNHFNVVPPDSEHYARARYRAIAARLYRALDDRLAAEEWLAGGDYSIA
                     DMATHPWAHYLERHGFDSADFPALLRWRTAIDERPATRRAKARVENEFLEKSNRSRQA
                     ATADDIDRFFNRTDEVPAVNYYGKTQ"
     gene            complement(922..1383)
                     /locus_tag="VN627_08900"
     CDS             complement(922..1383)
                     /locus_tag="VN627_08900"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXD03412.1"
                     /translation="MTQSDHPVRRVATGLDAAGRSCVSHEDAVPVAHPVVGLAWATRS
                     HPADNLAPCPPAAEWSELIHAGGTTFLHVRMPPGAGAPMHATDTIDYITMISGEMTIV
                     TETGEAVLRAGDVFVDRGVLHAWRNDGAQDAVYTSVTVAAHPVGAGRTDLP"
     gene            1896..2189
                     /locus_tag="VN627_08905"
     CDS             1896..2189
                     /locus_tag="VN627_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HXD03413.1"
                     /translation="MRQDTSGQSRFAGQIREHMEVIGADGVHVGTVDHVDGDRIKLTK
                     KDSGAGFEGGRHEGHHHYLSLGLVAEIEGNTVRLSANGDVAYGMEEEEGGEAS"
     gene            complement(2345..2713)
                     /locus_tag="VN627_08910"
     CDS             complement(2345..2713)
                     /locus_tag="VN627_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HXD03414.1"
                     /translation="MRKWHRWLVVVFGVFLVWIAVTGVLSQVVPWFVPRPQPAASAVP
                     AGFVCPEMLTCRPKSGGKSIVSLLHDLHSGASFGPIGTFIATLSGFAMVFFSLSGIWM
                     YVQMWRNRASRGAKPSWFWK"
     gene            complement(2874..4940)
                     /locus_tag="VN627_08915"
     CDS             complement(2874..4940)
                     /locus_tag="VN627_08915"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HXD03415.1"
                     /translation="MDFKSGDDRVSGDDVSGGAEASLFGDAAPAAASQTANQEAGASP
                     AVAMTNKTDAGSSELVEKLGADALAGALAGVMETAGRKKDDSKSVQPRRFRIVTDESR
                     DALLTDFGKDTLDDRYLLPGEKYQDLFARVADAYADDADHAQRLYDYVSKLWFMPATP
                     VLSNGGTGRGLPISCYLNSVSDSLEGIVGTWNENVWLASRGGGIGTYWGNVRGIGEPV
                     GLNGKTSGIIPFVRVMDSLTLAISQGSLRRGSAACYLDVSHPEIEEFLEIRKPSGDFN
                     RKALNLHHGVLLTDEFMEAVRDGAEFTLRSPKDGSERGTVDARSLFQKLVETRLATGE
                     PYIVFADTVNRMMPKHHRDLGLKVSTSNLCSEITLPTGRDHLGNDRTAVCCLSSLNLE
                     TWDEWNKDERFIEDVLRFLDNVLQDYIDRAPDEMARAKYSAMRERSVGMGVMGFHSFL
                     QLKGIAFESAMAKAWNLKMFKHVAAKADEASLLLAQERGACPDAADMGVMQRFSCKMA
                     IAPTASISIICGGTSACIEPIPANIYTHKTLSGSFVVENPYLKKLLAAKSKDSTNVWN
                     SILEHGGSVQHLDFLSPEEKAVYKTSFEIDQRWLLEFAADRTPYIDQAQSLNLYIPAD
                     VDKWDLMMLHFQAWERGIKSLYYLRSKSVQRAGFAGGVEADNTAAAPVIELATPTDYE
                     ECLACQ"
     gene            complement(4985..5242)
                     /locus_tag="VN627_08920"
     CDS             complement(4985..5242)
                     /locus_tag="VN627_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03416.1"
                     /translation="MRPIQDAALSRPVDDRVVAHANPVRFVAQALRAAGTDSAEGSRA
                     IGRRGKWNDIPVLFSRGEGLGRKLHQSSDSPGAAAGMVWSG"
     gene            5528..5785
                     /locus_tag="VN627_08925"
     CDS             5528..5785
                     /locus_tag="VN627_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXD03417.1"
                     /translation="MFNFLGAIVSGLFIGALARLFYPGPVHVGWLVTILLGVGGSLLA
                     NFVVRLRDPIAYDEGFTRAGCLASVLGAMVLIFLGRHFGWH"
     gene            complement(5946..>6065)
                     /locus_tag="VN627_08930"
     CDS             complement(5946..>6065)
                     /locus_tag="VN627_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03418.1"
                     /translation="QSADLIVKSALRRHESRGLHYTLDYPQTRAVAKDTVLVP"
BASE COUNT         1022 a         1978 c         2086 g          979 t
ORIGIN      
        1 aagatctggg tgagcaggtt gcgctcatgg tccgagatct tctgcgccca atcgcggcaa
       61 tcctcgccca aggggacttc ctcgggcatc cagtgcacct gctgctggcg cttccagaat
      121 tcgtaggccc agggatattc gaagggcttg taggtgttgc gtgcttcgag aaggggcatg
      181 atgggcctcg ttactgcgtt ttgccgtagt agttgacagc cgggacctca tcggtccggt
      241 tgaagaaacg gtcgatatcg tccgcggtgg ctgcctggcg gctccggttg gacttctcga
      301 gaaactcatt ctccacccgc gccttggcgc ggcgggtggc gggacgttcg tcgatcgccg
      361 tgcgccagcg gagcagcgcg gggaaatcgg cggaatcgaa cccgtgtcgt tcaagatagt
      421 gcgcccaggg gtgagtcgcc atgtcggcga tcgagtaatc cccacctgcg agccattcct
      481 ccgccgccag ccggtcatcg agcgcgcggt agagccgggc ggcgatggcg cggtagcggg
      541 cgcgggcgta gtgttcgctg tcgggcggca ccacgttgaa gtgattgacc tggccgaaca
      601 tgggcccgac gttggccatc tggatcatca gccactgcat gacctcggcc cgggcgggct
      661 gggcggcggg caggaaccgg ccctcgcgct cggccagcca gagcaggatc gcgcccgatt
      721 ccaccagcgg ccggccgagg cgcggatctt cgagcacggg caccttggcc agcgggctct
      781 tggcccggaa cccgggcgcg aactgctcgc cggtgaagac gccgacgtgg cgcagctcgc
      841 actccagggc gcactcctcg atcatcagga cgaccttgac gacattgggg ctgggcatgc
      901 cccagaggat caggggagcg gtcatggcag gtcggtcctt cccgcgccaa ccgggtgggc
      961 ggcgacggtg acgctggtat agaccgcatc ctgcgcgccg tcgttgcgcc aggcgtgcag
     1021 cacgccgcga tcgacgaaca cgtcgcccgc gcgcagcacg gcctcgccgg tctcggtgac
     1081 gatggtcatc tcgcccgaga tcatggtgat gtagtcgatc gtatcggtgg cgtgcatcgg
     1141 cgcgccggcg ccggggggca tgcgcacgtg caggaaggtc gtccccccgg cgtggatcag
     1201 ttcggaccac tcggcggcgg gcgggcacgg cgcgagattg tcggccgggt ggctgcgcgt
     1261 ggcccaggcg aggccgacca ccggatgggc gaccggcacc gcatcctcgt gcgagacgca
     1321 gctgcgcccc gcggcatcga ggccggtggc aacgcggcgg acggggtggt cggactgggt
     1381 catggcaggc tccttgcccg gcggaaacgc ggcccatcgc agcgctggcg cgggcgggcg
     1441 atggccggcg gcatcgggcg gaaccgaggg cgggcccggc gggtcatcct cgtgacgggg
     1501 ccggccagtc cccctcccgg ccccgcacct ttccaaggag atttacgatg ggtgagtacg
     1561 aacccaacga ttcgcgcaac gttaccggcg cgggcaacaa ggccggccaa aacgaaggca
     1621 acccgaacac caggcagggc ggccagcact ggcagcagag ttcgcaccag acccagccgg
     1681 gcgcgctgaa cgatccagcg cagaaccagc agaacccgat ctcgcagagc agccagcagg
     1741 gccacgggca ggacgggggc atgcgatcgc agcagcagca gttccagcag ggcggcgggc
     1801 agatgggcgg cgggcagagc gccaattccg gggccggcca gatgggcggc ggctcgggct
     1861 atcagggcac tgcggccggc ggccagaatg aatcgatgcg gcaggacacg agcggccaga
     1921 gccgcttcgc cgggcagatc cgcgagcaca tggaagtgat cggtgccgac ggcgtgcatg
     1981 tcggcacggt cgaccatgtc gacggcgacc gcatcaagct gaccaagaag gactccggcg
     2041 ccggcttcga aggcgggcgc cacgaagggc accatcatta cctctcgctc ggcctcgtgg
     2101 cggagatcga ggggaacacc gtgcgcctct cggccaatgg cgacgtggct tacggcatgg
     2161 aagaggaaga aggcggcgag gcttcctgac cgctgccgtt gcgcgggccc acccccagcc
     2221 cctcccgcct gcgggagggg agttgggcag cgccggtcac ccctcccgca ggcgggaggg
     2281 gtagcgagac ttggtgagcc gaaggcgagc ctagtcgcag cggggtgggc agcagcccgc
     2341 ccggctactt ccagaaccaa ctgggcttcg caccgcgcga ggcccggttg cgccacatct
     2401 gcacgtacat ccagatgccg gacaggctga agaacaccat cgcgaagcct gacagggtcg
     2461 cgatgaatgt gccgatcggc ccgaagctcg cgcccgaatg gagatcgtgg agcaggctta
     2521 caatagactt gccgccgctc tttggccggc aggtcagcat ttcggggcag acgaagcccg
     2581 ccggcacggc cgaggccgcc ggctgcggac ggggcacgaa ccagggaacg acctggctga
     2641 gcacgccggt caccgcgatc cacaccagga agaccccgaa caccacgacc agccagcgat
     2701 gccacttgcg catgaaccac gacccttccg tttgcgcgac ctgaagccgc ttcagcaagg
     2761 gtggttcccg tgggcaatcc gcaaagtgtc gcaggctgtc gcgcagacaa tgtcggggcg
     2821 cctgccggag agaagggcat gatcatcggc gatgtcctgc ccctcgcccg ccatcactgg
     2881 caggcgaggc actcctcata gtcggtcggc gtggcgagtt cgatcaccgg ggcggcggcg
     2941 gtattgtccg cctcgacccc gccggcgaac ccggcccgct gcaccgactt ggaccgcagg
     3001 taatacagcg acttgatccc gcgctcccag gcctggaagt gcagcatcat cagatcccac
     3061 ttgtcgacat cggccgggat gtagaggttc agcgactggg cctggtcgat gtagggggtg
     3121 cgatcggcgg cgaattcgag cagccagcgc tggtcgatct cgaagctcgt cttgtagacg
     3181 gccttttcct ccgggctgag aaaatcgagg tgctggaccg agccgccatg ctcgaggatc
     3241 gagttccaca cgttggtgga atccttcgac ttcgcagcga gcagcttctt gagatacggg
     3301 ttctccacga cgaacgagcc cgagagggtc ttgtgcgtgt agatgttggc cgggatcggc
     3361 tcgatgcagg cactggtgcc gccgcagatg atcgagatcg aagccgtggg cgcgatcgcc
     3421 atcttgcagg agaagcgctg catcacgccc atgtccgccg catccgggca ggcgccgcgt
     3481 tcctgggcca gcaggaggct cgcctcatcg gccttggcgg cgacgtgctt gaacatcttg
     3541 aggttccagg ccttggccat ggcgctctcg aaggcgatgc ccttcaattg caggaacgag
     3601 tggaagccca tcacgcccat gccgaccgag cgttcgcgca tggccgaata cttggcccgg
     3661 gccatttcgt ccggcgcgcg gtcgatatag tcctgcagca cattgtcgag gaagcgcagg
     3721 acgtcctcga tgaagcgctc gtccttgttc cactcgtccc aggtttcgag gttgagcgag
     3781 gacaggcagc agaccgccgt gcggtcgttg ccgaggtgat cgcgccccgt gggcagggtg
     3841 atttccgagc agaggttcga ggtcgaaacc ttgaggccga gatcgcggtg atgcttgggc
     3901 atcatccggt tcaccgtgtc ggcgaagacg atatagggct cgcccgtggc gaggcgggtc
     3961 tcgaccagct tctggaacag gctgcgggcg tcgaccgtgc cgcgctccga accgtccttg
     4021 gggctgcgca gggtgaactc ggccccgtcg cgcacggctt ccatgaactc gtcggtcagc
     4081 agcacgccgt gatggaggtt caaggccttg cggttgaagt ctccgctcgg cttcctgatc
     4141 tcgaggaatt cctcgatttc ggggtgcgag acgtcgagat agcaggccgc cgagccgcgc
     4201 cgcagcgacc cctgcgagat ggcgagcgtc aggctgtcca tcacgcggac gaaggggatg
     4261 atgccgctgg tcttgccgtt gaggccgacc ggctccccga tcccgcggac attgccccag
     4321 taggtgccga tgccgccgcc gcggctggcg agccagacgt tctcgttcca ggtgccgaca
     4381 atgccctcaa ggctgtcgga cacggaattg aggtagcagg agatcggcag gccgcgcccg
     4441 gtgccgccgt tggagagcac gggcgttgcc ggcatgaacc acagcttcga gacataatcg
     4501 tagagccgct gggcatggtc ggcatcgtcg gcataggcat cggccacgcg ggcgaacaga
     4561 tcctggtact tctcgccggg gaggagatag cggtcgtcca gcgtgtcctt gccgaaatcg
     4621 gtgagcaggg cgtcgcggct ctcgtcggtg acgatccgga agcggcgcgg ctggaccgac
     4681 ttggaatcgt ccttcttcct gcccgcggtc tccatcacgc cggcaagggc gccggcaagc
     4741 gcatctgcgc ccagcttttc gaccagctcg ctgctgcccg catcggtctt gttggtcatc
     4801 gctactgccg ggcttgcgcc cgcctcctgg ttggccgtct ggctggccgc tgccggggcc
     4861 gcatcgccga acagcgaggc ttcggccccg cccgacacgt catcacccga cacccggtca
     4921 tctccgctct tgaaatccac tgccgccccc attcatcaca aaccgctgac aaagcccttg
     4981 cggatcaacc gctccagacc attcccgctg ccgcgcccgg cgaatcggac gactggtgca
     5041 gtttacgccc caagccctcg ccgcgcgaga acaaaacggg aatatcattc cacttgcccc
     5101 tgcggccgat ggcgcgcgac ccttccgccg aatccgttcc cgctgcgcgc agggcctgcg
     5161 cgacgaagcg caccggattg gcatgagcaa ccacgcggtc atcgaccggc cttgatagtg
     5221 ccgcatcctg aatcgggcgc aaggggggta aggggccgct acccccaaga tgttgtgtgc
     5281 gagctcaagt tcaaccccca gccataccgt ctgggctctc gcgacgcgtg ggacatccgc
     5341 cactggcgcg ctgcgcaaga gtcaaagtga atctgtgaaa aaatttttgc cggtggacag
     5401 ggccgccagg cggttgacta cgaaaggaat cgggccgcgc aacatagttg ccaaattcgc
     5461 tcccggacca ttgcggcgcg gaggcgcgcg cctatcttgg cggccctgcc acaggagcgg
     5521 accaaccatg ttcaacttcc tcggtgcgat cgtctcgggc ctgttcatcg gcgccctggc
     5581 gcgcctgttc taccccggtc cggtccacgt cggctggctg gtgacgatcc tgctcggcgt
     5641 gggcggctcg ctgctggcga acttcgtggt gcgcctgcgc gatcccatcg cctacgacga
     5701 aggctttacc cgcgcgggct gcctcgcctc ggtgctgggg gcaatggttc tgatcttcct
     5761 ggggcggcac ttcggctggc attgagggct ggcacagggg agggctggca ccccgtcgtc
     5821 ccgggcttga cccgggaccg ctggcgaacc ttcaggcgag accgtgcgaa caggcgaggc
     5881 cacagcttcg catccgacac cggtcccgga tcaagtccgg gacgacgcga atgcgcaccg
     5941 ccgcctcacg gcaccagcac ggtatccttg gccaccgcgc gcgtctgcgg atagtccagc
     6001 gtgtagtgca ggccccggct ctcgtgccgg cgcagggccg atttcacgat caggtccgcg
     6061 ctctg
//
LOCUS       DATNBV010000372         4413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2773918,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000372 DATNBV010000000
VERSION     DATNBV010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2773918"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1706
                     /gene="cofH"
                     /locus_tag="VN627_08935"
     CDS             <1..1706
                     /gene="cofH"
                     /locus_tag="VN627_08935"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HXD03419.1"
                     /translation="HAAYGHIQEVIIQNFRAKERTPMAGAPEPDMDDLLWTIAAARLI
                     LGPAMNIQAPPNLSANDFGRLVEAGINDWGGVSPVTPDHVNPEAPWPEIARLREVTEA
                     QGRDLVQRLPVYPVWTGPGWIDPAVRPVLLAAADADYYARADSWSPGIALPPRDRLQP
                     RRPASPPVAALIERAMAGQPLSEGEIVTLFAARGDDVEHVCAAADELRRRVNGDTITY
                     VVNRNINYTNICTFRCGFCAFSKGDMAEELRGRPYDLDREEVIRRVREAWDRGGTEVC
                     LQGGIHPHYTGQTYIDLARAVIEAVPEMHIHAFSPLEVRQGAATLGLDLVTFLTRLKE
                     AGLGTLPGTAAEILDDEVRAIICPDKLTTAEWLEVVGTAHSVGLKTTSTIMFGHVDTP
                     RHWARHLIRLRDLQQRTGGITEFVPLPFVPMESPMALKGMTRMGPTFREVRLMHAVAR
                     LALHPHITSIQTSWVKLGADGVRAILDGGANDLGGTLMNESISRAAGAQHGQEMPPEE
                     MERLIRSIGRIPRQRSTLYGEVSEPVRARGFAAPELAPLVQTPPRRREKTQPKDALAD
                     V"
     gene            1699..2364
                     /gene="npdG"
                     /locus_tag="VN627_08940"
     CDS             1699..2364
                     /gene="npdG"
                     /locus_tag="VN627_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HXD03420.1"
                     /translation="MSDTPLATIAVLGGTGKEGGGLALRWAHKGHKVIIGSRSAERAQ
                     AAADEMNATLGSANASGATNPDAAAAADIVVLSVPFAGQLAAVEEVAEHLAGKILIDV
                     TVPLVPPKVSVVQLPNGGSAVAAVQEKLGEGVRVVSAFQNISAHHLAHLDEEMDCDVL
                     VCANDPAAADAVVELAREIGLRAWNAGPLANSVVAEGLTSILIALNRRYKVPGSGIRI
                     TGI"
     gene            2417..3187
                     /gene="cofE"
                     /locus_tag="VN627_08945"
     CDS             2417..3187
                     /gene="cofE"
                     /locus_tag="VN627_08945"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HXD03421.1"
                     /translation="MTAPAPVMLQPVPGIPLISSGDDLGAVLCGALDEAGLALADGDV
                     LVLAQKIVSKAEGRQVSLANVIPSPRAIEVAAECDKDPRLVELILSEADEIMRLRKGV
                     IIVRHRLGLVLANAGIDQSNVDHAGGPAALLLPLDPDATCQRLRAAISHRTGKAIGVV
                     IIDSLGRAWRSGTAGTAIGVAGLPALLDLRGRPDLYGRKLETSELGLADEIAAAASLV
                     MGQAAEGTPAVLVRGLSLPADPGSAADLIRPRALDLFP"
     gene            3184..4116
                     /gene="cofD"
                     /locus_tag="VN627_08950"
     CDS             3184..4116
                     /gene="cofD"
                     /locus_tag="VN627_08950"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HXD03422.1"
                     /translation="MIVVLAGGVGGARFANGLAALLPPGQLTVVVNVGDDFDHLGLRI
                     CPDLDTVTYTLANLNNRELGWGQLGETWNFMDALRKLDGPDWFNLGDRDLATHILRTH
                     RLNAGEPLSAIVADIARARGITQTILPATDDPLRTKVLTDDGELDFQDYFVRRRCEPV
                     FRAIRFAGAEQASAHPQVLAALADPAVEAILIAPSNPLLSLAPILAVSQIADALAART
                     VPCVAVSPFIAGQAVKGPAAKILRELGLKPGPAAIAAHYGALLDGLVIDQGDEPDLAA
                     PALLRTDILMQDDAGQQRLAREVLDFARDLGGGG"
     gene            4118..>4413
                     /locus_tag="VN627_08955"
     CDS             4118..>4413
                     /locus_tag="VN627_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03423.1"
                     /translation="MPARIVIPARAPEFGKSRLAAALAPAERTALALRLFDHVVRTAL
                     SADPSATVYVISNSPDLLARAQRHGAHALVERGEGLNRALEQAAGHLGGSDPLLT"
BASE COUNT          683 a         1681 c         1357 g          692 t
ORIGIN      
        1 tgcacgcggc ctatggccac atacaggaag tcatcatcca gaacttccgc gccaaggagc
       61 gcacgccgat ggccggggcc cccgagccgg acatggacga cctcttgtgg accatcgccg
      121 ccgcccgcct gatcctgggc ccggccatga acatccaggc cccgcccaac ctctcggcga
      181 acgatttcgg gcggctggtg gaagcaggga tcaatgactg gggcggcgta tccccggtca
      241 cccccgacca tgtgaacccc gaggctccct ggcccgaaat cgcccgcctg cgcgaggtca
      301 ccgaggcgca aggccgcgat cttgtccagc gcctgccggt ctatcccgtc tggaccggac
      361 ccgggtggat cgaccctgcc gtgcggccgg tcctgctcgc cgccgccgat gccgattact
      421 acgcgcgggc ggacagctgg tcgccgggca ttgccctccc cccacgcgat cgcctccagc
      481 cgcgccgtcc ggcaagcccc cccgtcgccg ccctgatcga acgggccatg gccggacagc
      541 cgctcagtga gggcgagatc gtcaccctgt tcgccgcgcg cggcgacgat gtcgaacatg
      601 tctgcgccgc ggccgacgaa ctgcgccgca gggtcaatgg cgacaccatc acctacgtcg
      661 tcaaccgcaa cataaactac accaacatct gcaccttccg ttgcgggttc tgcgcatttt
      721 ccaagggcga catggccgag gaactgcgcg ggcgccctta cgatctcgat cgggaggaag
      781 tgatccgccg ggtgcgcgag gcgtgggacc ggggcggtac cgaggtttgc ctccagggcg
      841 gcatccaccc ccactatacc gggcagacct acatcgacct cgcccgcgcg gtgatcgagg
      901 ccgtgcccga gatgcacatc catgccttct cgccgctcga agtgcgccag ggcgcggcta
      961 cgctcggcct cgatctcgtc acctttctca cccggctcaa ggaagccggt ctcggcacgc
     1021 ttcccggcac cgccgccgag atcctcgacg acgaagtccg cgcgatcatc tgcccggaca
     1081 agctcacgac cgccgaatgg ctggaagtgg tcggcacggc ccattcggtt ggtctcaaga
     1141 ccacctcgac gatcatgttc ggccacgtcg acacgccgcg ccactgggct cgccacctga
     1201 tccgcctgcg cgatctgcaa cagcggaccg gcgggatcac cgaattcgtg cccctgccct
     1261 tcgtcccgat ggaatcgccg atggcgctca agggcatgac ccggatgggg cccaccttcc
     1321 gcgaagttcg gctgatgcac gccgtcgccc gcctcgcgct ccatccccac atcacgtcaa
     1381 tccagacctc atgggtcaag ctcggcgccg atggggtgcg cgcgatcctc gatggcgggg
     1441 ctaacgacct gggcggcacc ctgatgaacg aaagcatctc ccgcgctgcc ggagcgcagc
     1501 acgggcagga aatgccgccc gaggaaatgg agcgcctgat ccgctccatc ggccgtattc
     1561 cgcgccagcg ttccaccctc tacggcgaag tgagcgaacc agtccgcgcc cgcggctttg
     1621 cggcgcccga actcgccccc ctggtccaga ccccgccgcg caggcgggaa aaaacacaac
     1681 cgaaggatgc ccttgccgat gtctgatacg ccactcgcca cgatcgccgt tcttggggga
     1741 accggcaagg agggtggcgg acttgccctg cgctgggccc acaagggcca caaggttatc
     1801 atcggcagcc gctcggccga acgcgcccag gctgccgccg acgagatgaa tgccaccctt
     1861 ggcagcgcca acgccagcgg cgccaccaac cccgatgccg ccgccgccgc cgatatcgtg
     1921 gtgctttccg tacccttcgc gggccagctc gcggcggtcg aggaagtggc cgaacacctc
     1981 gcgggcaaga tcctgatcga tgtcaccgtc cccctggtcc cgcccaaggt cagcgtggtc
     2041 cagctgccca acggcggctc cgccgtggcc gccgtccagg agaagctggg cgaaggcgtg
     2101 cgcgtcgtct cggcattcca gaacatctcg gctcaccacc tcgcccatct tgacgaagag
     2161 atggactgcg acgtgctggt ctgcgccaac gatcccgccg ccgccgatgc cgtggttgaa
     2221 ctcgcccgcg agatcggcct gcgcgcctgg aatgcggggc cgctcgccaa ctcggtcgtg
     2281 gccgaggggc tgacttcgat cctgatcgcg ctcaaccggc gctacaaggt cccgggctcg
     2341 ggcattcgta tcaccggcat ctgattgtct gccaagcgct tgctccggct cctcggggcg
     2401 cgctaggctt tgcgtgatga ctgctcccgc ccccgtcatg ctgcagcccg ttccgggcat
     2461 ccccctgatc tcctcgggcg acgatctggg cgcggtcctg tgcggcgcac tggacgaagc
     2521 tgggctggcg ctcgccgatg gcgacgtgct ggtcctggcc cagaagatcg tatccaaggc
     2581 cgaagggcgg caggtctctc tggcgaacgt cataccgtcg ccgcgcgcca tcgaggtggc
     2641 ggcggagtgc gacaaggacc cgcgcctcgt cgaactgatc ctgtccgagg ctgatgagat
     2701 catgcgcctg cgcaagggcg tcatcatcgt gcgccaccgc cttggcctcg ttctggccaa
     2761 tgccgggatc gaccagtcca acgtcgatca cgcgggaggc cctgccgccc tgctcctgcc
     2821 gctcgacccg gatgctactt gccagcgtct gcgcgcggcc atttcccacc gcaccggcaa
     2881 ggccatcggc gtggtcatca tcgacagcct tggccgcgcc tggcgcagcg gcacggcagg
     2941 gaccgcgatc ggcgttgccg gtctgcccgc cctgctcgac ctgcgtggcc gccccgatct
     3001 ctatggccgc aaactcgaaa ccagcgaact cggccttgcc gacgagatcg ccgccgccgc
     3061 ctcgttggtc atgggccagg ccgccgaggg cacgccggcc gtcctcgtcc gcggcctttc
     3121 cctgcccgcc gatcccggtt ccgccgccga tctgatccgt ccccgcgcgc tggacctgtt
     3181 tccatgatcg tcgtcctcgc cggtggggtc ggcggcgccc gcttcgccaa cggattggcc
     3241 gccctcctcc cgcccggcca gctcaccgtg gtcgtcaacg tgggcgacga tttcgaccat
     3301 ctcggcctgc gcatctgccc cgacctcgac acggtcacct acaccctcgc caatctcaac
     3361 aaccgcgaac tcggctgggg ccagctcggc gagacgtgga acttcatgga cgccctgcgc
     3421 aagcttgacg ggccagactg gttcaacctc ggcgaccgcg atcttgccac gcacatcctg
     3481 cgcactcatc gcctcaatgc aggtgagccg ctctccgcca tcgtcgccga tatcgcccgc
     3541 gcccggggca tcacccagac catcctcccc gcaaccgacg acccactgcg gaccaaggtc
     3601 ctcaccgacg acggcgaact ggatttccag gactatttcg tccgccgccg ctgcgagccg
     3661 gtgttccgcg ccatccgctt cgccggggcc gaacaggcca gcgcccaccc acaggtgctt
     3721 gccgccctgg ccgaccccgc ggtcgaggcg atcctgatcg ccccgtccaa cccgctgctc
     3781 agcctcgcgc cgatcctcgc cgttagccag attgccgatg cgctggccgc ccgcaccgtg
     3841 ccctgcgtgg ccgtctcccc attcatcgcc ggacaggcgg tcaagggccc agccgcgaag
     3901 atcctgcgcg aactcggcct taagcccggc cccgccgcta ttgccgccca ctacggcgcc
     3961 ctgctggatg gcctcgtcat cgaccaaggt gacgagcccg atctcgccgc cccggcactt
     4021 ctgcgcaccg atattctgat gcaggacgat gccggccagc agcgcctcgc gcgcgaagtg
     4081 ctcgacttcg cgcgcgatct tgggggcggc ggctagggtg ccggcacgga tcgtcatccc
     4141 cgctcgcgcg cccgagttcg gcaagagccg gctggccgca gcgctcgctc cggccgagcg
     4201 caccgccctc gccctgcgcc tgttcgacca tgtggtgagg acggcgctct cagccgatcc
     4261 aagtgcaacc gtctacgtca tcagcaacag tccagatctg ctggcccgcg cgcagaggca
     4321 tggggcgcat gcgctggtcg agcgaggcga aggcctcaac cgcgccctcg agcaggcggc
     4381 tggccacctc ggcggcagcg atccgctcct cac
//
LOCUS       DATNBV010000373         2322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_521262,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000373 DATNBV010000000
VERSION     DATNBV010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521262"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VN627_08960"
     CDS             complement(<1..210)
                     /locus_tag="VN627_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03424.1"
                     /translation="MSRRKDGKVIDFKLDEEFANKLVWVEQFVREKIEPLDALFPEHG
                     APWNPRDKVAMKIVRPLQAEVKAQGL"
     gene            383..1003
                     /locus_tag="VN627_08965"
     CDS             383..1003
                     /locus_tag="VN627_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXD03425.1"
                     /translation="MTAAVSDGESEQPRTLRDQQRELTRRRILDAAHKAFTEAGIATT
                     TVDQIARDAGISRATLYLHFSNREAILVELLRRDLRYVQTTFEALPRAVAGGHGPTRD
                     WLSAHAERHSRHRADLRLFSIATATDPVAVELVREHLVRVAGTVLGPGGEAIRSSDPA
                     LWTRVILMLMRINQVVASLAQSDPELDPATALDITAAEVIAVLGGR"
     gene            complement(1000..>2322)
                     /locus_tag="VN627_08970"
     CDS             complement(1000..>2322)
                     /locus_tag="VN627_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD03426.1"
                     /translation="RIAGQCIAAIGALEPRAGEPVIDAAGGALLPGLHDHHLHLFALA
                     AARSSVFCGPPEVAGCAALAARLAAAPGTGWLRGTGYHESVAGDLDRAWLDRHGPDRP
                     LRVQHRGGRRWIVNSRALDLLLAGNAAPPAGLDRARGHFDDEDGWLRARLAGQFPELA
                     PVSAQLAVCGITGVTEMTPANNGAALTFLAGQQALGSLRQRVVLAGCPELPDSASPQL
                     LVRYTKLHLHDARLPDYDDTCRLVVQSHGRGRPVAVHCVTLAELVFALAVLREAGPLA
                     GDRIEHAGVAPDEQVAEIAALGLIVVTQPNFIAERGDAYLADVDLAEQPFLYRGGAFL
                     SAGVTLAAGSDAPFGKADPWAAMAAAVSRRTAAGAVIGPEECLSPEQAVSLYLAEGAD
                     PRRLRSVTGGGPADLCLLDRSWAQARRRLDAAMVRLTIGAGTVLYERA"
BASE COUNT          381 a          836 c          766 g          339 t
ORIGIN      
        1 caggccttgt gccttgacct cagcctggag cgggcggacg atcttcatcg ccaccttgtc
       61 gcgcggattc cacggtgcgc catgctccgg aaacaatgca tcgagcggct cgatcttttc
      121 acgcacgaac tgttcgaccc acacgagctt gttcgcgaat tcctcgtcca gcttgaaatc
      181 gatcaccttg ccatccttcc gtctgctcac gccgtcccac aatctacaag cgaacccttg
      241 ctctggccgc tgccccttgc aggttacccg cgacccggtg ataagctcgc ccggctgaaa
      301 cgacctatgt acattggctg gacatatgtc tagtgaaatt ccatggcgct gaatcgcccg
      361 gcaggaaaga tcgccccacc tgatgaccgc agcagtctcc gacggtgaat ccgaacagcc
      421 ccgcaccctg cgcgaccagc agcgggaact gacccggcgc cgcatcctcg atgcggccca
      481 caaggcgttt accgaggcgg gaatcgccac gacaacagtt gaccagatcg cccgcgatgc
      541 cgggatcagc cgggccacac tctatctgca cttctcgaac cgggaggcga tcctggtgga
      601 gctcctgcgc cgcgacctgc gctacgtgca aaccactttc gaagccctgc cccgggccgt
      661 ggcgggcggc cacggtccga cgcgcgactg gctatcggcc catgccgagc gccactcccg
      721 ccatcgcgcg gacctgcgcc tgttcagcat tgccacggca acggacccgg tggcggtcga
      781 actggtacgc gaacatctgg tgcgggtggc gggaacagtg ctcgggccgg gcggggaggc
      841 catccgctcg tccgatccgg ccttgtggac ccgggtgatc ctgatgctca tgcggatcaa
      901 ccaggttgtt gccagcctcg cgcaatccga tccggagctt gatcccgcca ccgcgctgga
      961 cattaccgcg gcagaagtca tcgccgtgct cggcggacgc taggcccgct cgtaaaggac
     1021 ggtgcccgcg ccgatcgtca ggcgaaccat ggcggcatcc aaacggcgac gggcctgcgc
     1081 ccaactgcga tcgagcaggc acagatcggc gggcccgccg cctgtcacgc tgcgcaaacg
     1141 ccgtggatcg gcgccttcgg caaggtacaa cgagaccgcc tgctccggcg aaaggcactc
     1201 ctccgggccg atgaccgccc cggcagccgt gcggcgcgaa acggcggcgg ccatcgctgc
     1261 ccagggatcc gctttgccga acggcgcgtc gcttcccgcg gccaaggtaa cgccggcact
     1321 caggaaggcg ccgccgcgat agaggaacgg ctgctctgcg aggtcgacat cggccaggta
     1381 ggcatcgccg cgctcggcaa tgaagttggg ctgggtgacg acgatcaggc cgagcgcggc
     1441 gatctccgcc acctgctcgt cgggcgcgac cccggcatgc tcgatgcggt cgcccgccag
     1501 cgggcctgcc tcgcgcagca ccgcgagcgc aaagaccagt tcggccaggg ttacgcagtg
     1561 cacggccacc gggcggcccc ggccatggct ctgcaccacc agccggcagg tgtcgtcata
     1621 atccggcagc cgcgcgtcgt ggaggtgcag cttggtgtag cgcaccagca gctgcgggct
     1681 ggcactgtcc ggcagctcgg ggcagccggc gagcaccact cgctggcgca gtgatccgag
     1741 ggcctgctga ccggcaagga aggtgagcgc ggccccgtta ttggccgggg tcatctcggt
     1801 taccccggtt attccgcaga cggccagctg ggcgctgacg ggagcaagtt cgggaaattg
     1861 cccggccagc cgcgcgcgca gccagccatc ctcgtcatcg aagtgtccgc gtgcccggtc
     1921 cagccccgcc gggggcgccg cgttaccggc cagcagcagg tcgagcgccc ggctgttgac
     1981 gatccaccgc cgccctccgc gatgctgcac ccgcagcggc cggtccggcc catgacgatc
     2041 gagccaggcc cggtcgagat cgcccgccac gctctcgtga tagccggttc cccgcagcca
     2101 gcccgtcccc ggggcggcgg cgaggcgggc ggcaagggcc gcgcagccgg cgacttccgg
     2161 cgggccgcag aacacggagc tgcgggctgc cgccagggca aagagatgaa ggtgatggtc
     2221 gtgcaggccc ggcaacaggg ccccgcccgc cgcatcgatc accggctcgc cggcccgcgg
     2281 ctcgagcgcg ccgatggcgg cgatgcattg ccccgcgatg cg
//
LOCUS       DATNBV010000374         3112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1197732,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000374 DATNBV010000000
VERSION     DATNBV010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197732"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /gene="obgE"
                     /locus_tag="VN627_08975"
     CDS             complement(<1..969)
                     /gene="obgE"
                     /locus_tag="VN627_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXD03427.1"
                     /translation="MHFLDQAKIYVRAGSGGPGAVSFRREKYVEYGGPDGGNGGKGGD
                     IVFKAVAGLNTLIDFRYTQHFKAPRGVHGSGKNRNGAAGQDLVIKVPVGTQIISDEDR
                     ETVLADLTEAGQRVTLLEGGMGGRGNASYKTSTNRAPRQHQPGIPGEELWVWLRLKLL
                     ADAGLVGLPNAGKSTFINQITNTKAKVGAYAFTTLRPQLGVVRHREREFVLADIPGLI
                     EGAADGAGVGDRFLGHIERCRVLIHLIDVTGKDPVEAMQIVEDELEAYGAGLIDKPRL
                     VALNKIDLVDAELVQAFSEELIDAGAEAVFPISGATGAGLDALLDAV"
     gene            complement(1111..1722)
                     /locus_tag="VN627_08980"
     CDS             complement(1111..1722)
                     /locus_tag="VN627_08980"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXD03428.1"
                     /translation="MRLTRWTLALVALAAATPAAAKIYHLDGGTVRVRYGSSSSAPAS
                     RASERRTVVPADAPPPAVVDQPVSYQPSFESAAAAPMLDQAAAQAGLHPALLEALVWQ
                     ESRWHPGAVSRKGAIGLTQLMPGTARELGVDPRDPNANLLGGARYLRSLIDHFDGDLV
                     KGLAAYNAGARRVEQAGGVPPISETQQYVLAILRRVTATAGRR"
     gene            complement(1955..2030)
                     /locus_tag="VN627_08985"
     tRNA            complement(1955..2030)
                     /locus_tag="VN627_08985"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1995..1997),aa:Ala,seq:cgc)
     gene            complement(2126..2887)
                     /locus_tag="VN627_08990"
     CDS             complement(2126..2887)
                     /locus_tag="VN627_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03429.1"
                     /translation="MIGFNSKRVAALAGLSVLALLAGCGDKTPPAPPPPPPRPVAIVV
                     PPKPTPPDHAAPNLTVPPTDITGLRTSVNRNITPAQTVWNLRSAYNVAALNCPEPKYA
                     EIVVNYRAFLKAHVKGLAAFNRQVDAEFKSKYGAKFIAPREKYMTEVYNHFALPPTLD
                     AFCTAVLAVSRDAITVKPAELQPFAMRSLPNVEVVFDDFYRRYDAYRIALADWNQRYG
                     PPPTVINPGPVYVPPVQADGTLGGPGAVPVAPLGR"
     gene            complement(3018..>3112)
                     /locus_tag="VN627_08995"
     CDS             complement(3018..>3112)
                     /locus_tag="VN627_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011241146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HXD03430.1"
                     /translation="GKMIVMGALSLYLDFINMFTFLLQFMGDRR"
BASE COUNT          545 a         1001 c         1049 g          517 t
ORIGIN      
        1 caccgcatcg agcagggcgt cgagacccgc gccggttgcc ccggagatgg ggaacacggc
       61 ctcggccccg gcgtcgatca gctcctcgct gaacgcctga accaattcgg catcgaccag
      121 gtcgatcttg ttgagcgcca ccagccgcgg cttgtcgatc aggccggcgc cataggcctc
      181 gagctcgtcc tcgacgatct gcatcgcctc gaccgggtct ttcccggtca cgtcaatcag
      241 gtggatcaac acccggcagc gctcgatgtg gccgaggaag cggtcgccca cccctgcccc
      301 gtccgccgcg ccctcgatca ggccggggat gtcggcgagg acgaactcgc gttcacgatg
      361 acggacgacg ccaagctgcg ggcgcagggt ggtgaaggca taggcgccca ccttggcctt
      421 ggtgttggtg atctggttga tgaaggtcga cttgccggca ttgggcagac cgaccagccc
      481 ggcatcggcc agcagcttga gccggagcca gacccacagc tcctcgcccg gaatgcccgg
      541 ctggtgctgg cggggggcgc ggttggtgct ggtcttgtag gaggcgttgc ctcgaccacc
      601 cattccgcct tccagcaagg tgacccgctg gccggcctcg gtgagatcgg ccagaacggt
      661 ctcacgatcc tcatcggaaa tgatctgcgt gccaaccggc accttgatca ccagatcctg
      721 ccccgccgcg ccgttgcggt tcttgcccga gccgtggaca ccgcgggggg ccttgaagtg
      781 ctgggtatag cggaaatcga tcaaggtgtt gaggccggcg acggccttga acacgatatc
      841 cccgcccttg ccgccattgc cgccatcggg gccgccatac tcgacatatt tctcgcgccg
      901 gaaggacacg gcaccgggcc cgccggaacc ggcgcgcaca tagatcttgg cctggtcgag
      961 aaaatgcatg agcgagaaac tagcggttgc gcccggccat cggcaagctc aggcgaagcg
     1021 gagggaagca attgcagcgc ggatcggcgc aatggcctcg aacgccgcgc ctagctttcc
     1081 tgcggtgtac cgtcaacctt gccggtaatg ctatcgcctg ccggcggttg ccgtgaccct
     1141 gcggagaatg gccagtacat attgctgtgt ttccgaaatc ggcggcaccc cgccggcttg
     1201 ctcgacccgc cgcgcgcccg cgttgtaggc ggccaggccc ttgaccagat ccccgtcgaa
     1261 gtggtcgatc aggctgcgca ggtaacgcgc gccgcctagc aggttggcat tgggatcgcg
     1321 cggatcgact ccaagctctc gcgccgtgcc cggcatcagc tgggtgagac cgatggcgcc
     1381 cttccgcgag accgcgccgg gatgccagcg gctttcctgc cacaccagcg cctcgagcag
     1441 tgcagggtgg agcccggcct gcgccgcggc ctggtcgagc atgggcgcgg cggcggcgct
     1501 ctcaaaggag ggttgatagc tgaccggctg gtccactacg gcaggcggcg gagcatcagc
     1561 cgggacaacg gttcgcctct ccgacgcccg gctggccggc gcgctgctgc tgctgccgta
     1621 acgtacccgc acggtgccgc cgtcgaggtg atagatcttt gccgcagccg gagtggctgc
     1681 cgccagcgca accagtgcca atgtccaccg cgtcaatcgc atcgcaaatc ccgccagagc
     1741 gccctccccg ctcactagcg taacggggtt aacaggccgc tagcgggatc gctggacaga
     1801 atcggcgtgg gagattggac cagtgctttg gcaattgcct cagtccgaag cgaagatggt
     1861 gccttacaag aaccggcgcg cagcgaccat aatggggcac cggtacgcag gaaggtgccg
     1921 ttcgcagtct ggaatgtggc gattgacaaa gcactggtgg agccgagggg gatcgaaccc
     1981 ctgacctcgt cattgcgaac gacgcgctct cccagctgag ctacggcccc gttccagtgc
     2041 gagtgcggat ggtggccaag gcctgcctcc gcgggagcgg cccccttagc gaaggggtcg
     2101 cgccctgcaa agacaaattt tgccgctacc ggccaagtgg ggccaccgga acggcgccgg
     2161 gcccgcccag ggtgccatcg gcctgaaccg gcggcacata taccgggccc ggattgatga
     2221 ccgtcggcgg cgggccatag cgctggttcc agtccgccag ggcaatcctg taggcatcgt
     2281 agcggcgata gaaatcgtca aacacgactt ccacgttggg caggctgcgc atggcaaagg
     2341 gctgcaattc agccggcttt acggtgattg catcgcggct cacagcgagc acggccgtgc
     2401 agaaggcatc gagcgtgggc ggcaaggcga agtggttgta gacctcggtc atgtactttt
     2461 cgcgcggcgc gatgaacttg gcgccatact tgctcttgaa ctcggcatcg acctgccggt
     2521 tgaacgcggc aagccccttt acatgggcct tgaggaaggc gcggtagttg accacgatct
     2581 cggcgtactt cggctccggg cagttgagcg cggcgacgtt gtaggccgaa cgcaggttcc
     2641 acaccgtctg cgccggcgtg atgttgcggt tcacgctggt gcgcaggccg gtaatgtcgg
     2701 tcggcggcac ggtcaggttg ggtgcggcgt gatcgggcgg cgtcggcttg ggcggcacga
     2761 caatcgccac cgggcgcggc ggcggcggcg gcggagccgg cggggtcttg tcgccgcagc
     2821 cagccagcaa cgccaggacg gaaagacccg caagggcggc aacccgtttg ctgttgaacc
     2881 cgatcatttc atccctcccg cgcaagcgca gcatcggcgc gcggagaatg cctagtgatt
     2941 aatgcggtca aagaaaatgc ccggcgcgca cctggcgcgc cgggcaaatc gattttgcga
     3001 caagctgccg ccgcggttca gcggcgatcg cccatgaact gcagcagaaa ggtgaacatg
     3061 ttgatgaagt cgagatagag gctcagcgcg cccatcacga tcatcttgcc gg
//
LOCUS       DATNBV010000375         4097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1502351,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000375 DATNBV010000000
VERSION     DATNBV010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502351"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..493
                     /locus_tag="VN627_09000"
     CDS             200..493
                     /locus_tag="VN627_09000"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HXD03431.1"
                     /translation="MAAYVLLCNYTDQGIRAVKDSPKRRAAAKDLGKQLGVEVKTAYL
                     AMGTCDLILTVEAASDDAVAKWVLSLASKGNIRTTTIKVFPEAEMDKILAGLV"
     gene            1230..>4097
                     /locus_tag="VN627_09005"
     CDS             1230..>4097
                     /locus_tag="VN627_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXD03432.1"
                     /translation="MGSVSETQANGERRQVGVLFADLCGFTALSRRLDAEDLRRLVEA
                     FYARADAAIATYGGTVDKHIGDAVMALFGAPMAHGDDGFRALRAALDIAAATGEIAGP
                     AGEPLAAHVGVAMGEVVAGGIGRGYTVLGDAVNLASRLVNLAGPGEVVMAEDLKRQLE
                     GQVRAVALPPADLKGFAEPVTGWRAEGLETRARPASPYVGRETDRRLLANLLEACAMA
                     GRGRIVVLRGEAGIGKSRLVDETMAAAARAGFATHKALVLDFGAGRGLDARGMLTRSL
                     LDLSPDSDEAQRLIAAEKAAAGGSLAGDERLWLLDLLDLPAEGEDRALIQAMDEPTRL
                     GRRRAVLAALLSRLAHQRPLFAAVEDLHWADAVLLEDLAALGATTAQAPVLLLLSTRP
                     EGDRLDRAWRSKLGPAAVSTVDLAPLSAQESAALAAGYLDSGDQRIAACIERAGGNPF
                     FLEQLLRHTGEVANAAVPASVQSLVLGRADRLPAEEKRTLQAASVLGQRMSLEALRHL
                     IANPNYGAERLIDQQFMRREGNELAFVHALMRDGVYGSLLKARRRELHRAAAGWYGER
                     DLPLKAQHLDMADAPEAADAYLAAAESGAASYRTEQALSLARRGVALANDIATRVSLA
                     NLSGELLQGLGRTEEAIAAFRLAAEVATGGRPRLRSLLGLAHGLSVVVRFDEATALLD
                     GAQREAEDHGLLAEQSRIHTLRGNAHFPRGEIALCLAEHSQALHLAERSGAAEEQARA
                     LGGLADANYMRGLFRTAGQMFGRCVDVCAAQGFRRIEAANLPMLGMMTMMEMRFVEAV
                     KQLRRAAELAEQIGHRRAAMIASHGLAFVFHELAEQKAGLEAGMAALEIARSLGARRF
                     IAEGLMLKAQSEFVASDIQAAETILEANEIARETPKFLLPLGLALAARITRDPRERAV
                     ALTEGEAILAAGAVSHNYVFFNRYAIDACIAAEDWAGTERYA"
BASE COUNT          671 a         1326 c         1407 g          693 t
ORIGIN      
        1 gtccggtact gagccgcatc ggaatcgcct gattaacttt tttgagatgc cggctggacg
       61 gctgccggtg tggcatcttt aggcagcgac ggtggcagaa cgatggagcc ttcgcgaaag
      121 tcgagtttct gtttcatgcg ctccagcgtg agatcggcta taatggggtg agtacaaacg
      181 ctcaccaggg agggtttcca tggcagcata tgttctgctc tgcaactaca cggatcaagg
      241 aattcgcgcc gttaaagatt cgcccaagcg cagagccgcg gcaaaggatc tggggaagca
      301 gctcggcgtt gaggtaaaga ccgcctatct tgcaatgggg acgtgcgacc tcatcctgac
      361 tgtcgaagcg gcatctgatg atgccgtggc gaaatgggtt ctctccctcg cgtccaaggg
      421 aaacataagg accacgacga tcaaggtctt ccccgaagct gaaatggaca agattctggc
      481 agggctcgtc tgaaagctcg ttgaacgcat cacgcaggag atggccgcaa gcgtgagcgt
      541 catcatcggt cgcgctgaga ttggtgcatg gtcgcgcgtg tagttgtccc gagagtacct
      601 tactagggag ctgcagccga caacgtcggc ggccgggata gcaaccaatc ggcgttccgt
      661 aatctagtcc gcttccttct gcctggcgga ccagcggatc gaccgggtcg atccgaaaag
      721 gcaagcacct acccggttcc gaaggcgatc caccgcccac cgccgggtgc gatatggttg
      781 tctttccgct ggtggagcga ggtagcctta gatgaggcgg catcactctc acactcgtcg
      841 gcgccctgat cgaacaggcc gccagcggta tgaagtagcc ttgcccggag ccgcgcaccg
      901 acgcggcttc gcagccgtga gtgcaccgcg cctcgctgtc ccggtttccc gaagaccagc
      961 ccgcaagctg ccacctgaaa accccgcgta gattcgcctt gcttgccgcc ccgctggatg
     1021 gcgcctagca tggtccggcc tcacccgggg ttggagccat gcgctgcccg caatgcaatc
     1081 tggacaaccg cgagggacgg aagttttgcg ccaggtgcgg cggcgcgctg ggctggtcct
     1141 gcgcggcctg cggcttcgcc aatgacgccg gcgatcgatt ctgcggcggt tgcggtacgc
     1201 ccgcggcggc cccggcgtca ccgccggcaa tgggatctgt cagcgagacg caggcgaatg
     1261 gcgagcgccg ccaggtcggc gtcctgtttg ccgatctctg tggcttcacg gcgctctcgc
     1321 gccggctcga cgccgaggac ctccgacgtt tggttgaggc cttctatgcc cgcgccgacg
     1381 ccgccatcgc aacctatggc ggcacggtgg acaagcacat aggcgatgca gtgatggcgc
     1441 tgttcggcgc gccgatggcc cacggcgacg acggtttccg cgccttgcgc gcagcgctcg
     1501 acatcgccgc ggccacgggt gagatcgccg gccccgcggg cgaaccgctg gccgcccatg
     1561 tcggcgtcgc gatgggcgag gtggtcgccg gcggtattgg gcggggctac accgtgcttg
     1621 gcgatgcagt caatctggcc tcgcgcctgg ttaatctagc aggacccggc gaggtggtca
     1681 tggccgagga tctgaagcgc caactcgagg gccaggtccg cgcagtcgcc ctgccgcccg
     1741 ccgacctcaa gggcttcgcc gagccagtaa cgggttggcg cgccgagggg ctcgagacga
     1801 gggctcggcc ggccagcccc tatgtcgggc gggagacgga ccgccgcctg ctggcgaacc
     1861 ttctggaggc ttgtgccatg gcgggccgcg ggcggatcgt cgtgctgcgc ggggaggccg
     1921 gcatcggcaa gagccgtctg gtggatgaga caatggccgc ggcggcacgg gcagggttcg
     1981 ccacccacaa agccctggtc ctggacttcg gtgctggcag gggcctcgac gcccggggca
     2041 tgctgacccg tagcctgctg gacctctcgc ccgacagcga cgaagcgcag cgtctcatag
     2101 cagccgagaa ggcggcagcg ggcgggtcgc tcgccggcga cgagcgcctt tggctgctcg
     2161 atcttctgga cctcccggcc gaaggcgagg accgcgcact gatccaggcg atggacgagc
     2221 cgacgcgtct cgggcgccgg cgggcggtcc tcgcggccct gctcagtcgg cttgcccacc
     2281 agcgccccct attcgccgcg gtcgaggacc tgcattgggc cgacgccgta ttactggagg
     2341 atctggccgc gctgggtgcg acaaccgcgc aggcgcccgt gctgctgttg ctctcgacgc
     2401 ggcccgaagg tgaccgcctc gaccgcgcct ggcggtcaaa actgggaccc gcggcggtca
     2461 gcaccgtaga cctggcgccg ctctcggccc aagaatcggc ggctcttgcg gcgggttatc
     2521 tggatagcgg cgaccagcgg atcgcggcct gcatcgagcg cgccgggggt aatcccttct
     2581 tcctcgagca actgctgcgc cataccggcg aggtggcgaa cgccgccgtg ccggcctcgg
     2641 tacagagcct cgtgctgggt cgggccgacc ggcttccggc ggaggagaaa cgcacgctcc
     2701 aggcagcctc ggtactgggc cagcgcatgt cactggaggc gctgaggcac ctgatcgcca
     2761 atccgaacta cggtgccgag cggctgatcg atcaacagtt catgcgccgg gagggaaatg
     2821 agctcgcctt cgtccacgcc ctgatgcgag acggcgtcta tggctcgctg ctcaaggcac
     2881 ggcggcggga gctgcatcgt gctgccgccg gctggtatgg cgagcgcgac ctgccgctga
     2941 aggcccagca tctggacatg gccgatgcgc ccgaggccgc agacgcctat ctcgcggccg
     3001 cggaatccgg cgctgccagc tatcgcaccg agcaggcctt gtcactggcc cggcgcggcg
     3061 tggccctggc gaacgatatt gcgacccggg tatccctggc caatctgtcg ggcgaactgc
     3121 tgcagggcct gggccggacg gaagaagcca tcgcggcctt tcgcctggcc gccgaggtcg
     3181 ccacgggggg gcgcccgcga ttgcgctccc tgctcgggct ggcccatggt ctctcggtgg
     3241 tcgttcggtt cgacgaggca accgcgctgc tcgacggcgc ccagcgcgag gccgaggacc
     3301 acggtctcct cgccgagcaa tcgcgaattc atacgcttag gggcaatgcc catttcccgc
     3361 gaggcgagat cgcgctctgc ctcgccgagc acagccaggc tctgcatctg gccgaaaggt
     3421 ccggcgccgc ggaggaacag gcccgtgcgc tgggcggcct tgccgacgcg aattatatgc
     3481 gcggtctgtt ccggacggcg ggacagatgt tcgggcgctg cgtcgacgtc tgcgccgccc
     3541 aggggttccg tcgcatcgag gcggccaatc tacccatgct cggcatgatg acgatgatgg
     3601 aaatgcggtt cgtcgaggca gtcaagcagc tccggcgcgc cgccgagctg gccgagcaga
     3661 tcggccaccg ccgcgcggcg atgattgcca gtcacggcct cgcctttgtc tttcatgaac
     3721 tggcggagca gaaggccggg ctggaagccg gcatggcggc cctcgaaatc gcccgctctc
     3781 ttggtgccag gcggttcatt gccgaggggc tgatgctcaa ggcgcaaagc gaatttgtgg
     3841 cgagcgatat tcaggcggct gaaacaattc tcgaagccaa cgagatcgcc cgcgaaacgc
     3901 cgaaattcct gttgccgctg ggactagcgc tagcggccag gatcacccgc gatccccggg
     3961 aacgtgcagt tgctcttact gaaggcgagg cgatcctcgc ggccggcgcc gtcagtcaca
     4021 actacgtgtt cttcaatcgc tatgcgatcg acgcctgtat cgcagctgag gattgggccg
     4081 gcactgagcg ctacgct
//
LOCUS       DATNBV010000376         5695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2424602,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000376 DATNBV010000000
VERSION     DATNBV010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2424602"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VN627_09010"
     CDS             <1..211
                     /locus_tag="VN627_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXD03433.1"
                     /translation="REVPGLKKKPSTSELLDWLKLLLNEDMPLDVLQSQDPTKAIPPL
                     HGALLKNEQDIMMFERLAFMARRGA"
     gene            219..647
                     /locus_tag="VN627_09015"
     CDS             219..647
                     /locus_tag="VN627_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXD03434.1"
                     /translation="MSEHHGRCFCGGVTFTATAEPLGARMCWCRDCQYIASGSPTVNV
                     LFPEDAVTYAGPLTTLRKVADSGNTVQRGFCPTCGSQMYSRTLIPAGTQPVRVRAGTL
                     DDPSLCPPQAHIWVDSAPSWAMLDPALPRFRKGPGSERME"
     gene            652..1080
                     /locus_tag="VN627_09020"
     CDS             652..1080
                     /locus_tag="VN627_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXD03435.1"
                     /translation="MTYAGKCSCGAVCVAVSGEPAAVRQCWCRQCQQLAGGAATNNAM
                     FYTADVTVSGPLASAQWLAGSGNTLTHWRCAQCGNPVFAQSSARPQFMTLRLGIFDPG
                     HGLAPQAVIWTDEAPAWAAIDPALPAFPRQPPPPTPGAKA"
     gene            1083..2264
                     /locus_tag="VN627_09025"
     CDS             1083..2264
                     /locus_tag="VN627_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXD03436.1"
                     /translation="MFFNFLDELRAAGIPASLKEHLVLLEALDRQAIRQTPEEFYYLS
                     RATFVKDEGLIDRFDQVFAKVFKGIFSECGEQQFQLPEEWLKAVAEKFLTPEEMEKIK
                     SLGSWDEIMETLKKRLEEQEGRHQGGNKWIGTGGTSPFGNGGYNPEGVRIGGESKHKR
                     AIKVWEQREFKNLDSTKELGTRNIKVALRRLRRFAREGAAEELDLEGTINGTARQGWL
                     DIRMRPERHNAVKVLLFLDVGGSMDPYIKLVEELFSAATGEFKNLEFFYFHNCLYEGV
                     WKDNNLRYNQREKTWDVLHKYGHDYKVIFVGDAAMSPYEILQKGGAIDHYNEEPGALW
                     LERVTNTYPATVWLNPVEQRGWDYSQSTKIIRELMNDRMYPLTLSGLEAAMRELTRKR
                     G"
     gene            2298..3602
                     /locus_tag="VN627_09030"
     CDS             2298..3602
                     /locus_tag="VN627_09030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD03437.1"
                     /translation="MENRRLRSLLAVSLGNGLEMYDFTVFALFAIQIGKAFFPSHDPR
                     TSLLAAFATFGAGFLTRPLGAFVLGRLGDRRGRGPAMVLSMALMGLAVTMLALCPGYA
                     RIGLAAPLVALTARLLQGFALGGEVGPATAYLLECAPLHRRAVYVAVQRGTQLVANIC
                     GAVIGVVLASLLSPEVFATIGWRIAVGIGAVVAPFALYMRRNVPEPPAEPAALPCAGP
                     ALAPAAGAASLWRTAAACCLVGAGGTISAYVLSYMVTFGQADLHLPARVALAAQTAGC
                     AAGLVAVFGGAMMSDRWGRRRLILLANLLAAALVPLVFLALLQAPRPAEFILATAAVG
                     ALLNFGTGAVITVSTESFPRHVRSMATALAYTVPVTVFGGTTQFAVAWLIGATGSAMV
                     PALYAAAALLLSSLALLFLRETAPVRLGLLSNRNEVPLGAHV"
     gene            3595..4401
                     /locus_tag="VN627_09035"
     CDS             3595..4401
                     /locus_tag="VN627_09035"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HXD03438.1"
                     /translation="MSDRQPALFVGHGSPMTMITDNVERRVLAEIGAGLLRPRAVLCL
                     SAHWETRGQTHVTAAGHPRTVHDFRGFPQELFDWRYSAESPAWLVDRIVALLGADRVR
                     ADDSWGYDHGTWGVLDLLFPGEHLPTVAMSLDRTLDAAGFLALGAALAALRDEGVMIV
                     GSGNVIHNLSLWRASAGTVPQWADEFRHRINRAMTAGDRAALTSFAPDDEPAAKAINS
                     AEHYLPLLAVAGARLPGDKVGIFNDTVDGALSMTSYYLGDAATAPAAIAF"
     gene            complement(4535..>5695)
                     /locus_tag="VN627_09040"
     CDS             complement(4535..>5695)
                     /locus_tag="VN627_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HXD03439.1"
                     /translation="KPLAGKRVALIAHPASVTDRLVHSLDALAACPDLKLSAAFGPQH
                     GLKGDKQDNMVETADEIDPLHGIPVFSLYGEVRRPTARMMDSADVFLFDLQDLGCRIY
                     TFVTTLLYLLEEAAKAGKSVWVLDRPNPAGRPVEGTTLLPGQESFVGAGPMPMRHGLT
                     LGEMGHWFVRHFGLDVDYQVIAMEGWAPEAGPGWGWPESRIWVNPSPNAANLNMARAY
                     AGTVMLEGTTLSEGRGTTRPLEVLFGAPDVDARAVLGEMERLAPEWLAGCAIRECWFE
                     PTFHKHAKSLCSALMIHAEGRFYNHFAFRPWRLQALAFKAIRNLWPDYPLWRDFPYEY
                     EFERLAIDVINGGPALREWVDDRSALAGDLESLAAADEAAWRAEIGDFLLYR"
BASE COUNT          924 a         2119 c         1709 g          943 t
ORIGIN      
        1 ccgcgaggtc cccggcctca agaagaagcc ctcgacctcc gaactgcttg actggctcaa
       61 gctcctcctg aacgaggaca tgccgctcga cgtcctgcag tcgcaggacc cgacgaaggc
      121 catcccgccc ctccacggtg ccctgctcaa gaacgaacag gacatcatga tgttcgaacg
      181 cctcgccttc atggcgcggc gcggggcgta accggcgcat gagcgagcac cacggccgct
      241 gcttctgcgg cggggtaacc ttcaccgcca ctgccgaacc gctcggcgcg cgcatgtgct
      301 ggtgccgtga ttgccagtac atcgccagcg gctcgcccac ggtgaacgtc ctgttccccg
      361 aagacgcggt gacttacgct ggcccgctca ccacgctgcg caaggtcgcc gacagcggca
      421 ataccgtcca acgcggcttc tgtcccacct gcggctcgca gatgtacagc cgcacgctca
      481 ttcccgccgg cacccagcca gtccgggtcc gggcgggcac gctggatgat ccgtcgctat
      541 gcccgccgca ggcccatatc tgggtagaca gcgcgccatc gtgggccatg ctcgaccccg
      601 ccttgccccg cttccgcaag ggcccggggt ctgagcgcat ggagtgatcg catgacctac
      661 gccggcaagt gttcctgcgg agccgtttgc gtcgcagtgt ccggcgaacc tgccgcggtc
      721 cgccaatgct ggtgccgcca atgccagcaa ctcgccggcg gtgccgccac caacaacgcc
      781 atgttttaca cggccgacgt gaccgtatcc ggcccgctgg cctcggcgca atggctggcc
      841 ggctcgggca acaccctcac gcactggcgc tgcgcgcagt gtggtaaccc ggtcttcgcg
      901 caaagctcgg cccgccccca gttcatgacc ctgcgcctcg gcattttcga tccgggccac
      961 ggccttgccc cgcaagccgt gatctggacc gacgaggccc ccgcctgggc ggcgatcgac
     1021 cccgcgctcc ccgccttccc ccgccaaccg cccccgccaa cccccggcgc gaaagcctga
     1081 ccatgttctt caacttcctc gatgaattgc gcgccgccgg cattccagcc agcctcaagg
     1141 agcacctcgt cctgctcgag gcgctggacc ggcaggcgat ccggcagacg cccgaggaat
     1201 tctactatct ctcccgcgcc accttcgtga aggacgaggg cctgatcgac cggttcgatc
     1261 aggtcttcgc caaggtcttc aagggcatct tctccgaatg cggcgagcag cagttccagc
     1321 tgcccgagga atggctcaag gctgtcgccg agaagttcct cacgcctgag gagatggaga
     1381 agatcaagtc gctcggctcg tgggacgaga tcatggagac gctgaaaaag cggctggaag
     1441 agcaggaagg ccgtcaccag ggcggcaaca agtggatcgg caccggcggc accagcccct
     1501 tcggcaacgg cggctacaac ccggaaggcg tgcgcatcgg cggcgaaagc aagcacaagc
     1561 gcgccatcaa ggtctgggag cagcgcgagt tcaagaacct cgacagcacg aaggaactcg
     1621 gcacccgcaa tatcaaggtc gccctgcgcc gcctgcgccg cttcgcccgc gaaggcgcgg
     1681 ccgaggagct cgatctcgaa ggcacgatca acggcaccgc ccgccagggc tggctcgata
     1741 tccgcatgcg gcccgaacgg cacaacgccg tcaaggtcct gctgtttctc gatgtcggcg
     1801 gctcgatgga cccctacatc aagcttgtgg aagagctgtt cagcgccgcc accggggaat
     1861 tcaagaacct cgaattcttc tacttccaca attgcctcta tgaaggcgtg tggaaggaca
     1921 acaacctgcg ctacaaccag cgcgagaaga cgtgggacgt gctccacaag tacgggcacg
     1981 attacaaggt gatcttcgtc ggcgatgccg cgatgagccc ttacgagatc ctccagaagg
     2041 gcggcgcgat cgatcactac aacgaggaac ccggcgcgct gtggctggag cgggtcacca
     2101 acacctatcc cgccaccgtc tggctcaacc ccgtcgagca acgcggctgg gactattcgc
     2161 agtccacgaa gatcatccgc gaactgatga acgaccggat gtatccgctc accctctcgg
     2221 ggctcgaggc ggccatgcga gaactcaccc gcaagcgcgg atagcgacca ctggtgaggc
     2281 cgggggattt gcggggcatg gaaaaccgaa gactgcgatc cctgctggcc gtgtcgctgg
     2341 gcaatggcct ggagatgtac gatttcaccg ttttcgcgct gttcgcgatc cagatcggca
     2401 aggccttctt tccctcccat gatccgcgca ccagtctgct cgcggccttt gccaccttcg
     2461 gggccggctt cctgacgcgc ccgctcgggg ccttcgtgct cggccggttg ggcgaccggc
     2521 gcggacgtgg gccggcgatg gtgctgtcga tggcgctgat gggccttgcc gtgaccatgc
     2581 tggcgctgtg cccgggctat gcccggatcg gccttgccgc cccgctcgtc gccctgaccg
     2641 cccggctgct gcaaggcttc gcgctcggcg gcgaagtcgg cccggccacg gcctacctgc
     2701 tcgaatgcgc gcccctgcac cgccgcgccg tctatgtcgc ggtccagcgc gggacccagc
     2761 tcgtggccaa catctgcggc gccgtcattg gcgtcgtcct cgcctccctg ctctccccgg
     2821 aagtctttgc caccatcggc tggcgcatcg cggtcggcat cggcgcggtg gtcgcccctt
     2881 ttgccctcta catgcgccgc aacgtccccg aaccgccggc ggaacccgcc gccctgccat
     2941 gcgccggtcc ggctctcgct cccgcggctg gcgcagccag cctctggcgc acggccgcgg
     3001 cctgctgcct cgtgggcgcg ggcggcacca tctctgccta tgttctgtcc tacatggtaa
     3061 ccttcggcca ggccgatctc cacctccctg cgcgggtcgc ccttgccgca cagaccgccg
     3121 gctgtgccgc cgggctcgtc gccgtgttcg gcggcgccat gatgtccgac cggtgggggc
     3181 gccgccgcct gatcctgctc gccaacctgc tggcggcggc gctggtgccg ctggtcttcc
     3241 tcgccctcct gcaggcgccc cgtcccgccg aattcatcct cgccacggcc gccgtgggcg
     3301 cgctgctcaa tttcgggacc ggcgcggtca ttaccgtgag caccgaaagc ttcccccgtc
     3361 acgtgcgctc catggcgacc gccctggcct acactgtccc ggtaaccgtg ttcggcggaa
     3421 ccacgcaatt cgcggtcgcc tggctgatcg gcgcaaccgg ttccgccatg gttccggcgc
     3481 tctatgccgc cgccgccttg ctgctcagca gcctcgccct gctgttcctg cgcgagaccg
     3541 cgccggtccg cctcggcctc ttgtcaaatc gcaacgaagt gccacttggc gcccatgtct
     3601 gatcgccaac ccgccctgtt cgtcggccat ggctcaccga tgaccatgat taccgacaac
     3661 gtggaacgcc gcgtgctcgc cgaaattggc gccgggctcc tgcgtccccg cgcagtcctg
     3721 tgcctgtccg cccactggga aacgcgcgga cagacccatg tcacggcggc gggtcacccg
     3781 cgcaccgtcc acgatttccg cggcttcccg caggagctgt tcgactggcg ttattccgcc
     3841 gaaagcccgg cctggctggt tgaccgcatc gtggccctgc tcggggccga ccgcgtccgg
     3901 gcagacgaca gctggggcta cgatcacggc acctggggcg tgctcgacct gctgttcccc
     3961 ggcgaacact tgccgaccgt cgccatgagc ctcgatcgca ccctcgacgc ggcgggcttc
     4021 ctcgcgctcg gcgctgccct cgcggcgctg cgtgatgagg gcgtgatgat tgtcggcagc
     4081 ggcaatgtca tccacaacct ctcgctgtgg cgggccagtg ccggaacggt gccgcagtgg
     4141 gccgatgaat tccgccaccg gatcaaccgg gcgatgaccg ccggcgatcg cgcggccctg
     4201 accagcttcg cacccgatga tgaacccgcc gccaaggcca tcaacagcgc cgagcattac
     4261 ctcccgctgc tcgccgtcgc cggcgcccgc cttcccggcg acaaggtcgg catattcaac
     4321 gataccgtcg acggcgccct gtcgatgacc tcctattacc tcggcgatgc ggcgacagcc
     4381 ccggccgcca tcgcgttctg accggccggg attttcctac acggccaaat cctgtcacac
     4441 tcggcaggcg ccattccaac cttcgcccgc agccccgccg ccctgccccc tttatgcaac
     4501 ggcggctcca gcaagtatct tgtcaaccaa accctcagcg atagagcaaa aagtccccga
     4561 tttccgcgcg ccaggccgcc tcgtccgccg ccgcgagtga ctcgagatca ccggccagcg
     4621 cagaacggtc atcaacccat tcccgcaagg ccggtccgcc gttgatcacg tcgatcgcca
     4681 gccgctcgaa ctcgtattca taaggaaagt cccgccacag cggatagtcc ggccacaggt
     4741 tccggatcgc cttgaacgcc agcgcctgca gccgccacgg cctgaaggcg aaatgattat
     4801 agaaccgccc ctcggcatgg atcatcagcg ccgaacacag gctcttggca tgcttgtgaa
     4861 aagtcggctc gaaccagcac tcccgaatcg cgcagcccgc cagccattcc ggcgcgagcc
     4921 gctccatctc gcccagcact gccctggcat ccacgtccgg cgcgccgaac agcacttcca
     4981 gcggccgcgt cgtcccgcgc ccctccgaga gcgtcgtacc ctccagcatg accgtccccg
     5041 cataggcccg cgccatgttg aggttcgccg cgttcgggct tgggttcacc cagatccggc
     5101 tctccggcca gccccagccc ggccctgctt ccggcgccca gccttccatg gcgatgacct
     5161 ggtagtccac atcaagcccg aaatggcgga cgaaccagtg ccccatctcg cccagcgtca
     5221 gcccgtgccg catcggcatc ggccccgcgc cgacaaagct ctcctgcccg ggcagcaggg
     5281 tggtcccctc gacgggccga cccgccgggt tgggccggtc cagcacccac acgctcttcc
     5341 cggccttggc cgcctcttcc aggaggtaga gcagggtcgt cacgaaggta tagatccggc
     5401 agccaaggtc ctgcaggtcg aacaggaaaa cgtcggcact atccatcatc cgcgctgtcg
     5461 gccgccgcac ctcgccatag aggctgaaca ccgggatacc gtgaagcgga tcgatctcgt
     5521 ccgccgtctc caccatgtta tcctgcttgt cgcccttcag cccatgctgc ggcccgaatg
     5581 cggcgctgag cttaaggtcc gggcatgccg ccagggcatc gagcgaatgg accagcctgt
     5641 cagtcacgga ggccggatgg gcgatgaggg ccacgcgctt gccggctaac ggctt
//
LOCUS       DATNBV010000377         5693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1800094,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000377 DATNBV010000000
VERSION     DATNBV010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800094"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            595..1350
                     /locus_tag="VN627_09045"
     CDS             595..1350
                     /locus_tag="VN627_09045"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HXD03440.1"
                     /translation="MNARPAAPVLGQAVRRLRAMALAAADGAFLGSEEEMRALLDVSR
                     PTLRQAARILEQERLIEMRMGKRGGIYAARPDLQTVARSAAQYLQIEGTTLLHLLNVY
                     HGLREQVTTAACGCTDDAMRERLAAFLAGVSASLPAENTSLFLEHERRFEQLLVDMAG
                     NPALGLFTRIARRFVDENPASQVFIARPEVRRSRCQAWLSLGSAVLAGDRARAIELGS
                     EQQRAFVAMIPGESLHDMGIGGSDLPASAAAAR"
     gene            complement(1426..3438)
                     /locus_tag="VN627_09050"
     CDS             complement(1426..3438)
                     /locus_tag="VN627_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXD03441.1"
                     /translation="MSFQLPATVPATALVSLALAASPAIAAAQPAPDEDAPIVVTGRG
                     LDLPPAAAAYDAVELPAERLRQSASGRIEDALSAVAGFQQFRRSDSRSANPSAQGVTL
                     RALGGNATSRTLVLLDGVPQADPFFGYIPLSAIDPGSLAAARVTRGGAGAFGAGAVAG
                     TIELISAGPAELGRASGETMVDDRGDTDLSVRLAPRLGDGFAVVAGQRNRGPGFWTTP
                     ESQRGPASVRAAYRTWSVGARAVAPLAGDVELQARLAAFDDARTLRFAGADTTSSGED
                     ASLRIVGRGVLPFEAMAYLQARNFTNVVISSTTFRKTLDQYATPSTGWGGKLELRPRL
                     LASHLVRLGADWRSAEGTAYEATYNAVTGALTGRRSSGGRNTDLGLFAQDDWTLGALV
                     LTGGLRADRAVAGNRSGWEFSGRGGAVLRLDHGLALRGSAYSGIRQPTLNELYRSFTL
                     TAPGIRTNTLANPDLGNERLEGFEAGADLSAGAGLSFSFTLFDNRVRQAIANVTIGQP
                     TVVGGTLVTNRKRLNVTAIHARGLEAGAELKAGAFTFSGSLALTEARVEAGGLAAQLS
                     GRRPAQTPELAASATLGWHPRPGLAVALTLKHVGAQYEDDLETDLLPAATTLDGFVEL
                     PLRPGVSLVLRGENLTDKAVLTRNQGGSYDLGAPRTLWAGLRAQIR"
     gene            complement(3573..4610)
                     /locus_tag="VN627_09055"
     CDS             complement(3573..4610)
                     /locus_tag="VN627_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HXD03442.1"
                     /translation="MHDARVLVVDDSAAMRALFSDVLEQAKNVEVVGAAANADDARQQ
                     IATLKPNVLTLDVEMPGMSGIEFLAEIMQTAPMPVVMLSSLTQAGTETSLKAYDLGAV
                     ECFPKPLKATPEQFAKTVGKLGKIVLAAANSNLRDRQASNARRTAEPVAPSFAWNGHI
                     VAMSASLGGIDALSGLLAAYPANCPPTVIVLSAEPAVVDAFIARFAGEGGCSVRAAKD
                     GAQLTQGVVHIASDPSRHVLFEAGQPPRLKLIDREPVEGARPSANLLYGTIARAGVPA
                     VGVVLTGMGEDGAKGLKLLRDAGSDTIAQDPASATFGEAPAAAIAAGGAGQVVALDGL
                     AAAVLGACSMA"
     gene            4804..>5693
                     /locus_tag="VN627_09060"
     CDS             4804..>5693
                     /locus_tag="VN627_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HXD03443.1"
                     /translation="MNLQRKIQIGGWMILAALALALVVAAIGIQEIRLGGPPHRAEQT
                     MSTLEADVLPPPLFIVEPWLEATLAVDQRARMDDHLANMARLRKEYDARKAYWQEAPI
                     SADLRREVDASDSTVDAFWTVVDTQFVPALRSGDPARIDEAHANLAAAFAQHRKAIDS
                     LVAKVQAAKTASASSASTMLIVTLSLLSIVAAGIVALVLYSRRHLQQEAIDPLAHTAA
                     AMSRMAAGDLEASAGIDADRADEIGDLNRALAVFRQSALDQRAAEQAQREVVEALSAA
                     LHQLADGDLGHAISTAFAPE"
BASE COUNT          989 a         1895 c         1924 g          885 t
ORIGIN      
        1 gtagtggaag tggaggtcgg cgcgcgctca cggcgccgga cagcaggttt ggtcatcgga
       61 aaaatctcca cgaacgcccg gagaccggcg ggcaccacga acggcacacc cgcctgaagc
      121 gaaatgaagc cgataccgtg ctgctgcgtt cgaagagatg ctgctgctca gaagcgccga
      181 gtctcctcgg cgcagcaacc aagtagcacc ttgactttgc cagcgcaagc atcaatccaa
      241 agttttgccg atcttctcaa gtacttgacg aaggattttc tgtacagacc ttcagatccg
      301 cccttcccgt aacgacttta ctaaattccc attggatgtt cgatcgtgga gggcgaaaac
      361 acaggtgata gtagggttcc cgctgcctcg agcgtactct aggctatcgg gctgaaatgg
      421 atttcccgtg gaaattccct ttgaacggga atttcctact gagacgattg gtctgggtgg
      481 tggccacacc tccaccctca aactcctcgc catcaagccc tgcagctcgc ggccggtatt
      541 gaaacgtcgc ctgcacgccg cgatagtccc gccggcaatg aggagaattt acctgtgaac
      601 gcgcgtccgg cagcgccggt cctgggtcag gcagtgcgca ggttgcgggc catggcgctc
      661 gctgcggcgg atggcgcgtt tcttggctcc gaggaggaga tgcgggcctt gcttgacgtg
      721 agccggccaa cactgcggca ggcagctcgc attctcgagc aggagcgatt gatcgagatg
      781 cggatgggca agcggggcgg aatctatgcc gcgcggccgg acctgcaaac cgtggcccgt
      841 tccgccgcgc aatatctgca gatcgagggt acgaccctgc tgcacctgct caacgtctat
      901 catggtctgc gcgaacaggt gaccaccgca gcttgtggct gcacggacga cgcaatgcgc
      961 gaaaggctgg cggcatttct tgccggcgtc tcggccagcc tgcccgccga gaacaccagc
     1021 ctgttcctgg agcacgagcg ccgcttcgaa cagctgctgg tggatatggc gggcaacccc
     1081 gcgcttggcc tgtttacgcg gatcgctcgc cggttcgtcg acgagaaccc cgcgtcccag
     1141 gtgtttatcg ccaggccaga ggtgcgccga tcgcggtgcc aggcatggct gagcctcggc
     1201 tcggccgttc tcgccggtga tcgtgcgcgg gcaattgagc tcgggagcga gcagcaacgc
     1261 gcgttcgtag ccatgatccc gggagaatct ctgcacgaca tggggatcgg cggatccgac
     1321 cttccggctt cagcggccgc ggcaaggtag tcgccctaaa tcgccggaca agctcgggcg
     1381 gcgatcagga caagagtggg agccgcccat cgccgaagct gcgcgttacc tgatctgcgc
     1441 tcgtagcccg gcccacagcg tgcgcggggc acccagatca tacgacccgc cctggttgcg
     1501 ggtgagcacg gccttgtcgg tgaggttttc cccgcgcagg acgagcgaga cgcctggcct
     1561 gagtggaagc tcgacaaagc catcgagcgt ggtggccgcc ggcagcaggt ctgtttcgag
     1621 gtcgtcctcg tactgcgcgc cgacgtgctt gagtgtgagt gctaccgcaa ggccgggccg
     1681 cgggtgccac ccgagcgtcg cgctggcggc aagctccggg gtctgcgcgg ggcgcctgcc
     1741 gctaagctgg gcggccagtc cgccggcctc gacccgggcc tcggtcagcg ccagcgagcc
     1801 ggagaaagtg aatgcccccg ccttcagttc ggccccggct tccagtccgc gggcgtggat
     1861 cgcggtgacg ttgaggcgct tccggttggt taccagtgtg ccgccgacga cagtcggctg
     1921 gccaattgtc acattggcga tcgcctgccg tacccggttg tcgaacagcg taaacgagaa
     1981 cgacaggcct gcgcccgcag agagatcggc ccccgcctcg aacccttcga gacgttcgtt
     2041 gcccagatca ggattggcca gggtgttggt ccggatgccg ggcgcagtga gcgtgaaaga
     2101 acggtagagt tcgttgagcg tcggctgacg gatgccgctg taggccgagc cgcgcagggc
     2161 gaggccgtga tccagccgca gcacggcccc gccgcggcca gaaaactccc acccggagcg
     2221 gttgcccgcc acggcgcggt cagcccgcag gccgccggtc aggaccagtg cgccgagcgt
     2281 ccagtcatcc tgcgcgaaga ggccaagatc ggtattgcgt ccgccgctgc tccggcgacc
     2341 ggtgagcgcg cccgttacgg cattgtaggt cgcctcgtag gccgtgccct cggcgctgcg
     2401 ccagtcggca cctagccgga caaggtggct cgcaagcagg cgggggcgca gttccagctt
     2461 gccgccccac cccgtcgagg gggtggcata ctggtcgagc gtcttgcgga aagtggtcga
     2521 cgaaatcacg acattggtga agttgcgcgc ctggaggtag gccatggctt cgaacggcag
     2581 gacgccacgg ccgacgatcc ggaggctggc atcctctcct gacgaagtgg tatcggcccc
     2641 ggcgaagcgc agcgtgcgag cgtcgtcgaa ggcggcaagc cgcgcctgca gttcgacatc
     2701 gcccgcaagc ggcgcgaccg cgcgggcacc aaccgaccag gtgcgatagg ccgcgcggac
     2761 gctggccgga ccgcgctggc tttccggcgt ggtccagaac cccggcccgc ggttccgctg
     2821 gcccgcgaca acggcgaagc catcgcccag ccgcggggcc agacggacgg agagatcggt
     2881 gtccccgcga tcatcgacca tcgtctcgcc actggcgcgg ccgagctcgg cagggccggc
     2941 gctgatcagt tcgatcgtgc cggcaaccgc gcccgcgccg aaggcgccgg ccccgccccg
     3001 ggtaacccgc gccgcggcga ggctacccgg atcgatggcc gacagcggaa tatagccgaa
     3061 gaacggatcg gcctggggca cgccgtccag caggaccagc gtgcggctgg tggcattgcc
     3121 gcccagcgca cgcagggtca ccccttgggc ggacggattg gccgagcggc tgtccgagcg
     3181 gcggaactgc tggaacccgg caacggcgga gagggcatcc tcgatccggc ccgaggcgga
     3241 ctgcctgagc cgttcggcgg gaagctccac cgcatcgtag gccgcagccg ccgggggcag
     3301 gtcaagcccg cgccccgtga cgacgatcgg agcatcctca tcaggggcgg gctgcgcagc
     3361 ggcgatggcc ggactggcgg caagggcgag cgatacaagc gccgtggccg gcacggtggc
     3421 gggtagctga aaggacaagc ggggctccaa gctgaggtgc cgcccgccgg acaccagccc
     3481 aaacccgcga acgcaagcac ttggctggcc gctctagcgc gaaccccgcg aagcgcaagc
     3541 atgcttgccc catcttggaa gtggcagtgc gttcaggcca tggagcaggc gccgagcacg
     3601 gcagcagcca gaccatcaag cgcaacaact tgccccgcgc cgccggcggc gatggcagct
     3661 gccggagcct cgccgaatgt agcggaggcg gggtcctggg cgatggtatc gctccccgca
     3721 tcgcgcagca gcttgagacc cttggcgccg tcttcgccca tgccggtgag gaccacgccc
     3781 acggcaggaa cgccggcgcg ggcaatggtg ccatacagga ggttggccga gggccgcgcg
     3841 ccttccaccg gctcgcggtc gatcagcttg aggcgcggcg gctgcccggc ctcgaagagg
     3901 acgtggcgcg acggatcgga agcgatatgg acgaccccct gggtcagctg tgccccgtcc
     3961 ttggccgcgc gcaccgaaca gcccccttcc ccggcaaacc gggcgatgaa ggcatcgacc
     4021 accgccggtt cggccgacag cacgatgacc gtgggcggac agttcgcagg gtaggcggca
     4081 agcaaaccgc ttagcgcgtc aattcccccc agcgaggcgc tcatcgcgac aatgtggccg
     4141 ttccaggcga agctgggggc gaccggctcg gccgtgcggc gggcattgct ggcctggcga
     4201 tcacgcaggt tgctgttggc ggcggcgagt acgatcttgc cgagcttgcc gaccgtcttc
     4261 gcaaactgct cgggcgtcgc cttgagcggc ttgggaaagc attcgaccgc acccagatca
     4321 taagccttca gcgaagtttc cgtacccgct tgggtaaggc tggaaagcat cacgaccggc
     4381 atcggggcgg tttgcatgat ctcggccagg aactcgatcc cgctcatccc gggcatttcg
     4441 acgtccagcg tcaggacgtt gggcttgagc gtggcgatct gctggcgggc atcgtccgcg
     4501 ttggccgcgg ctcccacgac ttcgacgttc ttcgcctgct cgagcacatc gctgaacagc
     4561 gcgcgcatgg cggccgaatc gtcaactacc agtactcttg catcgtgcat ggcgaccaca
     4621 tgtccctcga ttggtgtgcg gccatgctag cgtccgaacg atagcgaagg gtcaccgccc
     4681 actagggaga aatccgggtc ccaccgcaaa gcctgtgttg gcgggccctg gcctaagccg
     4741 gtcagcgaca cccagatcgc aacgccaagt tggccgagac agcgacaacc ggaaaaaatg
     4801 cacatgaacc tgcagcgcaa gatccagatc ggcggctgga tgattctcgc cgccctggcc
     4861 ctggccctcg tcgtggccgc gatcggcatc caggaaatcc gccttggcgg gcctccgcac
     4921 cgggccgaac agacgatgag cacgctggaa gccgatgtgc tgccgccgcc gctgttcatc
     4981 gtcgaaccct ggctcgaagc caccctggcg gtcgaccagc gcgcccgcat ggacgaccac
     5041 ctcgccaaca tggcgcgcct gcgcaaggaa tatgacgccc gcaaggccta ttggcaggag
     5101 gcaccgataa gcgctgatct gcgccgcgag gtagacgcct cggacagcac ggtcgacgcc
     5161 ttctggacgg tcgtggacac ccagttcgtg ccagccctgc ggtccggtga cccggcacgg
     5221 atcgatgaag ctcatgcgaa cctggcagcg gcgttcgcgc agcaccgcaa ggccatcgat
     5281 tcgcttgtcg ccaaggtcca ggccgccaag acggcaagtg ccagctcggc atcgaccatg
     5341 ctgattgtca ccctgtcgct tctttccatc gtggcggccg ggattgtcgc cctggtgctc
     5401 tacagccgcc gccatctgca gcaggaggca atcgatccgc tggcccatac ggcagcggcc
     5461 atgagccgga tggccgcggg cgatctcgag gcgagcgccg ggatcgacgc cgaccgggcc
     5521 gacgagatcg gcgacctcaa tcgcgccctg gccgtgttcc gccagtccgc gcttgaccag
     5581 cgcgccgccg agcaggccca gcgcgaggtc gtcgaggcgc tgtccgctgc tctgcaccag
     5641 ctcgccgacg gtgaccttgg ccatgcgatc agcaccgcat tcgcgcccga ata
//
LOCUS       DATNBV010000378         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4689479,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000378 DATNBV010000000
VERSION     DATNBV010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4689479"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1817)
                     /locus_tag="VN627_09065"
     CDS             complement(<1..1817)
                     /locus_tag="VN627_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HXD03444.1"
                     /translation="MTGTPSRPAAGSPATARVAARRQRIVGTRRRYNQWVNDQTLEDY
                     ALRFTARRARIWSAGWVGNTALGSIAFLACEAIGATVTGSYGFAASAWALGTAALIFV
                     LAGLPVCYYGARYGIDMDLLTRGSGFGYLGSTITSAVYASFTFILFAIEAVILSAALN
                     LCFGLPLALGHIISAMVVIPIAAYGFRRISRWQNWTQPLWLVLQFLPLLFIAGQGEAG
                     WRLWTGFPGRAGELHLHSFAAALAVFLSLLPQIGEQVDYLRFLPERETIGARRWWSAL
                     LLTGPGWIAMGAVKIALGSYLAVIALAAGVSASESTNPTALYFLAFSGLAPQPWLAVA
                     LTGLFVTICQLKINVTNAYAGSIASSNFFSRLTHRHPGRVVWLVFNTLIALMLMEGGI
                     QTVVERVLALYSNFAVAWFGAVVADLVINKPLGLSPPGIEFRRAILYDINPVGVGAML
                     GSLVCSTLCFAGLFGPTLQMFSPVVGLAAAFLLSPLIAWATGGAFYIARRAEPLDAHV
                     DADGQCGCTVCENAFEVPDMVLCPVYQGPICSLCCTLEARCHDACKEVAPEASQGMGP
                     RLRAILPVAMSDVFYLRLVRFVSGYAMASVVVSLVLLLT"
     gene            complement(1822..2379)
                     /locus_tag="VN627_09070"
     CDS             complement(1822..2379)
                     /locus_tag="VN627_09070"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HXD03445.1"
                     /translation="MPKIRTRPLAAMALSAAILAPTAAMAHPGHGLALSAGFADGLVH
                     PWTGLDHLVSLLLAGAWAAVLGGRALLRVPVTLLLGMLAGFVAAPLAGAGVAEALAAG
                     ATISLVVVAALRVRAPLPLAMSACALFGFGHGLAHGLESTGSDGAFAAGMVAGSSLLM
                     ALGLAGAQWALRTPALCALTLPARR"
     gene            complement(2379..>2576)
                     /locus_tag="VN627_09075"
     CDS             complement(2379..>2576)
                     /locus_tag="VN627_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXD03446.1"
                     /translation="RSDLLVINKIDLAPMVGASLEVMDRDARKMRGERPFVFTNVREG
                     KGVTDIAQFIAKTGGLPERSL"
BASE COUNT          489 a          934 c          842 g          311 t
ORIGIN      
        1 taggtcagca ggagcacgag gctgaccacc acgctcgcca tggcatagcc gctgacgaag
       61 cggaccagcc gcaggtagaa cacatcgctc atcgcgaccg gcagaatcgc ccgcagtcgc
      121 ggccccatac cctggctggc ctccggcgcc acctccttgc aggcatcgtg gcagcgcgcc
      181 tctagcgtgc agcacagcga gcatattggc ccctggtaga ccgggcagag caccatgtcc
      241 ggcacttcga aggcattctc gcagaccgtg cagccgcact gcccgtccgc atcgacatga
      301 gcgtcgagcg gctcggccct gcgcgcgata tagaaggcgc ctcccgttgc ccaggcgatc
      361 agcggcgaga gcaggaaagc cgcggccagg ccgacgaccg gcgagaacat ctgcagcgtc
      421 ggcccgaaca gcccggcaaa gcacagcgtc gagcagacca gcgagccgag catggcgccc
      481 acgcccaccg gattgatgtc gtacaggatc gcccggcgga attcgatgcc cggcgggctc
      541 aggcccagcg gcttgttgat caccaggtcg gccaccaccg caccgaacca agccacggcg
      601 aaattgctgt agagcgccag cacccgctcg accacggtct ggatgccgcc ttccatcagc
      661 atcaacgcga tcagcgtatt gaacaccagc cagacgacgc ggcccggatg gcggtgcgtc
      721 aggcgcgaga agaagttgga tgaggcgatg gagccggcat aagcattcgt cacgttgatc
      781 ttcagctggc agatcgtcac gaacagcccg gtcagggcta cggccagcca cggctgcggg
      841 gccagccccg aaaaggcgag gaaatagagc gcggttggat tggtcgattc gctcgccgaa
      901 actccggccg ccagcgcgat caccgccagg taggaaccca gcgcgatctt gaccgcgccc
      961 atcgcgatcc agcctgggcc cgtcagcagc agggccgacc accagcgccg cgctccgatc
     1021 gtctcgcgct cgggcaggaa ccgcaggtaa tcgacctgct cgccgatctg cggcaggagc
     1081 gagaggaaca ccgccagagc cgcggcaaag ctgtgcaggt gcagttcgcc ggcgcggccc
     1141 ggaaagcccg tccacaaccg ccaccccgcc tcgccctgtc cggcgatgaa cagcagcggc
     1201 aggaactgca gcaccagcca cagcggctgg gtccagttct gccagcggct gatccggcgg
     1261 aacccgtagg ccgcgatcgg gatcaccacc atggcgctga tgatgtgacc cagcgccagc
     1321 ggcaggccga agcacaggtt gagcgcggcc gagaggatca ccgcttcgat cgcaaaaagg
     1381 atgaaggtga acgaggcgta taccgccgag gtgatcgtcg atcccagata gccgaagccc
     1441 gacccgcgcg tgagcaagtc catgtcgatg ccgtagcgcg cgccgtaata gcacaccggc
     1501 agccccgcca ggacgaagat cagcgccgcc gtccccagcg cccaggccga ggccgcaaag
     1561 ccatagctgc ccgtaaccgt cgcgccgatc gcctcgcagg ccaggaaggc gatcgagccg
     1621 agcgccgtgt tgccgaccca cccggccgac cagatccgcg cccgccgcgc cgtgaagcgc
     1681 agcgcgtagt cctcaagggt ctggtcgttg acccactggt tatagcgccg ccgggtcccg
     1741 acaatacgct gccgccgcgc cgcaacccgc gccgttgccg ggctgccggc agcaggacgg
     1801 ctcggtgtcc cggtcaatgc gtcagcgccg cgccggcaag gtgagggcgc aaagggcagg
     1861 cgtccgcagc gcccactgcg ccccggccag ccccagcgcc atcagcagcg aactgccggc
     1921 taccatgccg gccgcaaagg ccccgtccga tcccgtgctt tccaggccat gcgctagccc
     1981 gtggccgaag ccgaacagcg cgcaggcgct catggccagc ggcagcggcg cccgcacccg
     2041 cagcgcggcc acgacgacga gcgaaatggt cgcccccgcc gctagcgcct cggccacgcc
     2101 cgcccccgcc agcggcgcgg cgacgaagcc cgccagcatc ccgagcaaca gcgtcaccgg
     2161 cacgcgcagc agggcgcgcc cgcccagcac ggccgcccag gcgccggcca gcagcaggct
     2221 caccaggtgg tcgagcccgg tccacgggtg gaccaggcca tcggcaaagc cggccgaaag
     2281 ggccagtcca tgccccgggt gcgccatggc cgcagtcggc gcgagaatgg cggcggacaa
     2341 ggccatggcg gcaagcggac gcgtgcggat cttcggcatc agaggctcct ttccggcagt
     2401 ccgccggtct ttgcgatgaa ctgtgctatg tcggtcacgc ctttcccctc gcgcacgtta
     2461 gtgaacacga agggccgttc gccgcgcatc ttgcgggcgt cgcggtccat gacctcaagg
     2521 ctcgcgccga ccatcggcgc aaggtcgatc ttgttgatga cgaggagatc ggaccg
//
LOCUS       DATNBV010000379         2399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2139522,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000379 DATNBV010000000
VERSION     DATNBV010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139522"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..963)
                     /gene="paaG"
                     /locus_tag="VN627_09080"
     CDS             complement(160..963)
                     /gene="paaG"
                     /locus_tag="VN627_09080"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HXD03447.1"
                     /translation="MQVSGETVLVAKSGGVTRLTLNRPDKLNAFTTGMHAELRAALEA
                     AGSDGQCRAVVLTGAGRAFCAGQDLAETRPAGDGVVPDGGSRLEQWYNPLVRLITTLE
                     KPVIAAVNGIAAGAGANVALACDLVFAARSASFLQAFTRIGLVPDASGTWTLPRLVGP
                     ARARGLTMLAEPLPAEKAEAWGLIWKVVDDDKLEAEVAAVAGKLALAPTYALGLTKRA
                     IAQSSTNTLAQQLDLERDLQRLAGASPDAAEGIRAFLEKRPPKFTGRSA"
     gene            1240..>2399
                     /locus_tag="VN627_09085"
     CDS             1240..>2399
                     /locus_tag="VN627_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03448.1"
                     /translation="MRCNWRRWLWGIIPLLVLSWVAVQAEHSRLERDLAERARLALVE
                     GGFTWAQAEFKARDALLMGRAPREGEPGKAADALAAVWGVRVVDNKADLLDKAEKYVW
                     TASRRNNRIRLSGYAPSIDTRRAILGVTKASFPGFEIVDRTTLARGSPSSDVWLAGVS
                     FALKQLTAIKRGDVRLEDLGLTDTGQAEDVTAYRTIKQALANSVPKGISVTADLVTAP
                     VASPYMWAAHSADGRLVLSGHVPNDAVRADLVAAAKEGLPRAAIVDQMQPGEGAPQGW
                     VDIAVAILREMGRLQGGGADMKDATLVVSGLAADAATAEAIRATLRAAVPATIKLTDQ
                     IQVKEPAPPALPTPSVAPPPTVVPPERAETPAKGPEAASSARTDTAAQSTPPP"
BASE COUNT          377 a          831 c          827 g          364 t
ORIGIN      
        1 agaagccatg cccgttgacc atgttgtcgc ccacgctcat ggagagaacg gcaaagcccg
       61 gccccacctt ctcgactttc attcccatgg cgcgggaagc cttgtcttcc gcccacatgg
      121 cgtccgcgct cgcctgtgcg acctcggccg cgtcgcgcgt catgcgcttc tcccggtgaa
      181 tttcggcgga cgcttttcga ggaacgcgcg gatgccctcg gcggcatcgg gcgaggcccc
      241 ggccaggcgc tgcaagtcgc gctccagatc cagctgctgg gcgagcgtgt tggtcgagga
      301 ttgggcgatg gcgcgcttgg tgaggcccag cgcataggtg ggcgcgagcg cgagcttgcc
      361 cgccaccgcc gctacctctg cctcgagctt gtcgtcgtcg acaaccttcc agatcagccc
      421 ccacgcctcg gccttctcgg ccggaagcgg ctcggccagc atggtgagcc cgcgggcgcg
      481 cgccgggccg acgaggcgcg gcagcgtcca cgtgccgctg gcgtctggga cgagaccgat
      541 gcgggtgaag gcctgcagga agctcgcact gcgcgccgcg aacaccaggt cacaggcgag
      601 cgcaacgttg gcgccggcgc cggccgcaat gccgttgacg gcggcgatca ccggcttttc
      661 cagtgtcgtg atgagccgaa cgagcgggtt gtaccattgc tcgagcctgc tgccgccatc
      721 gggcacgacg ccgtcgccgg caggacgggt ctcggccaga tcctggccgg cgcagaaggc
      781 acgtcccgct cccgtcagca cgaccgcccg gcattgccca tcgctgcccg cagcttccag
      841 cgccgcccgc agctcggcgt gcatgcccgt cgtgaacgca ttgagcttat cgggccggtt
      901 gagggtcagc cgggtgacgc caccgctctt tgcaaccaag actgtctcgc cgctcacctg
      961 cattccctcc ctgatccata gaggcccgat gttgtagccc agcggcccac gtgcacaaac
     1021 aggcgttgcg ggcctgtcgg cgggggtcgc gagccggctg cggccgcttg gaatgccggg
     1081 gccggttgtc tatagtgccg ccacaaaaga gcccagggca atcctcggtg acggtcaagc
     1141 cgtgcaggcc accctctgct ggcttcggca tggcgtcggg tgagcgacgc gggagctttc
     1201 agacgggcaa atcgccacga tctcgggagc gaaacacgca tgagatgcaa ttggcggcga
     1261 tggctttggg gcatcatccc gctgctcgtt ctgagctggg tggctgtcca ggccgagcac
     1321 agccgattgg agagggacct ggccgaacgc gccaggctcg cgctggtcga gggcgggttc
     1381 acctgggccc aggctgaatt caaagcccgc gacgctctgc tgatgggccg ggcgccgcgg
     1441 gagggcgagc ccggcaaggc ggccgatgcg ctggccgcgg tatggggcgt tcgcgtcgtc
     1501 gacaacaagg ccgacctgct cgacaaggcc gagaaatacg tctggaccgc gagccgccgg
     1561 aacaaccgca ttcgcctttc ggggtatgcg cccagcatcg atacccggcg ggccatcctg
     1621 ggtgtgacga aggcgagctt tccggggttc gagatcgtcg accgcacgac gctggcgcgc
     1681 ggcagtccgt cctctgatgt ctggctggcc ggcgtcagct tcgcgctgaa gcagttgacc
     1741 gccatcaagc gcggcgacgt ccgcctggaa gacctgggcc tcactgacac gggccaggcc
     1801 gaggatgtca cggcttaccg caccatcaag caggcgctcg ccaacagcgt gcccaagggc
     1861 atcagcgtga cggccgacct cgtcaccgca ccggtggcga gcccttacat gtgggccgcg
     1921 cactcggccg atggtcggct cgtgctgtcc ggccacgtgc cgaacgatgc cgtgcgggcc
     1981 gacctggtgg ccgccgccaa agagggcctg ccccgtgccg cgatcgtcga tcagatgcag
     2041 ccaggcgaag gcgcaccgca gggctgggtc gacattgcgg tcgcaattct gcgcgagatg
     2101 ggccggctcc agggtggtgg cgctgatatg aaggatgcca cgctggtcgt ctccggcctg
     2161 gccgcagatg cggcgacggc cgaagccatt cgtgcaacgc tgcgcgcggc cgtgccggcg
     2221 acgatcaagc tcaccgacca gattcaggtg aaggagccag cgccgcccgc actgccgacc
     2281 ccaagcgtgg cgccgcctcc aaccgtggtg ccgccggagc gcgccgaaac gccggccaag
     2341 ggacccgagg cggcaagttc cgcccgcacc gacaccgcag cgcaatccac cccgccgcc
//
LOCUS       DATNBV010000380         4157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4051643,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000380 DATNBV010000000
VERSION     DATNBV010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4051643"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VN627_09090"
     CDS             <1..390
                     /locus_tag="VN627_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HXD03449.1"
                     /translation="ICPGAKFNGVDDADPAETYAALLRALDPLGLAWCHLIHIPTTGF
                     DALDLVRANWSGAVIENCGLTLEKAQEVIAQGSVEAVAFGYAFIGNPDLVERFRIGAP
                     LAKADRGTFYTGHGDDAKGYTDYPRAT"
     gene            complement(413..808)
                     /locus_tag="VN627_09095"
     CDS             complement(413..808)
                     /locus_tag="VN627_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD03450.1"
                     /translation="MTDAPGQAAMKQALQAYVDRINAGDAAGVLALFAPDAVIEDPIG
                     SPPRSSEAIAAWFADTVAYGTRITPVAPLRGSHGSEALLVFDVEFTPPGGERLRIRSA
                     DACQFDAAGRITSLRAFWGPDDIESAASD"
     gene            complement(907..3483)
                     /locus_tag="VN627_09100"
     CDS             complement(907..3483)
                     /locus_tag="VN627_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXD03451.1"
                     /translation="MNKKAKGLRARSGWVNWGVAAIAIMAPEMAAAQASSTADASTAP
                     EIIVTANRRAERAQDVPIAITAMTPQRLEQQGINKEQDLQASVPSLVVGANGQGSRDS
                     QSFTIRGQGATFQASPGVVVYLNEVPLPAALTLSQQGGPGNFVDLENLQVLSGPQGTL
                     FGRNTTGGAVLLVPKKPTNELGGWIKGEYGNYNRTYIEGAINLPVIEDKLMVRVVGAY
                     HRRDGYTHDVIWNKDRDNENWYSGRIGILFRPTDGIENYTMIYGAHSDNNGAGLIHKG
                     FNIDGLKAIVNPADGSPAPACYEGPTIPFLIASCDVYRANTARADALGPRATAFSVDE
                     FQRTKTWGVTNTTNIELSDNVTLRNIASFQRMQLRYRYDGDATALQQHEVDPGVLPSG
                     TVDVPGLGPTTYGNATLARELPRDDLKGWTEELQFQGKMLDNKLNWTVGGFHYDQRPA
                     GPQGSNAILYCPAAFTGNAFVCGGSTQISGITTVSNALYAQATLDFGAFSPALDSLRL
                     TGGYRHTWDHVYGTASQYNRSPLLPAGFVACGKDNSIVPESSALASCVFDGSEKSDSP
                     SWLIGLDYRPVKPVLLYAKVSRSYKAGGFNPYAVFFGQGTDPDTRTFKPEHVISYEAG
                     FKADFRLGNVPVRFNTSLYEIHYNDIQRATGDYNPTTGAGGARTLNADAKIRGIEIEG
                     SIRPWPGVEIGGNFSHTDAHYTKYSFVTGTGGLGCNGIVPPGGTVDASCLRFQYTAPY
                     IWSLHASVDQPLAGNAGTLSLFVNYSHTASQYTEAVNVPAVQPGATIDAFGTLNASLD
                     WRGVMGSKFDVGVFGTNITNELYRTSNADVYQQGGLLYWVTLYGEPRMYGVRLKYHFG
                     GE"
     gene            complement(3925..>4157)
                     /locus_tag="VN627_09105"
     CDS             complement(3925..>4157)
                     /locus_tag="VN627_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HXD03452.1"
                     /translation="VVGMADERMGEVGKAYVVLRPGAEIPSEADLIAWSRQNMANYKV
                     PRSFVVLPELPRNAAGKVLKTELRSPPAPLAD"
BASE COUNT          733 a         1292 c         1362 g          770 t
ORIGIN      
        1 atatgcccgg gggccaaatt caacggcgtc gacgatgccg atccggcgga aacatacgcc
       61 gcgcttttgc gcgctctcga cccgcttggc ctcgcctggt gccacctgat ccacattccc
      121 accactggct tcgacgcgct cgacctcgtt cgcgcgaact ggtcgggcgc cgtgatcgag
      181 aactgcggcc tgacgcttga gaaggcgcaa gaggtgatcg cgcaaggttc ggtcgaagcc
      241 gtcgccttcg gttatgcctt catcggcaac cctgatctgg tcgagcgatt ccgcatcggc
      301 gctccgctcg ccaaggcgga tcggggaaca ttctacacag gccatggtga tgatgccaaa
      361 gggtacaccg attacccccg ggcgacgtga cggctgccgt gttcctcgcc agctagtctg
      421 aggccgccga ctcgatatcg tccgggcccc agaacgcccg caggctggtg atccgacctg
      481 ccgcgtcgaa ctggcaggca tcggccgaac ggatccggag acgctcaccg cccggagggg
      541 tgaactccac atcgaacacc agcagggcct cggaaccgtg cgacccgcgg agcggcgcga
      601 cgggggttat ccgcgtgccg taggccaccg tatcggcaaa ccaggcggcg attgcttcac
      661 tcgagcgtgg tggcgatccg atcggatcct cgatcacggc atcgggcgca aacagggcca
      721 ggacacccgc cgcgtcgccg gcgttgatgc ggtcgacata ggcctggaga gcctgtttca
      781 tcgcggcctg cccgggcgca tcggtcatat tcggttcctt cccacaacga acgaagtccg
      841 ccccccgcga agggagcgga ccttgcgtcc ctgtcaccgg cccgccatca ggcggacccg
      901 cgcaattcac tcgccgccga agtgatattt caggcggacg ccgtacatgc gtggctcgcc
      961 gtagagcgtc acccagtaga gcagcccgcc ctgctgatag acgtcggcat tgctggtccg
     1021 gtagagttcg ttggtgatgt tggtgccgaa cacgccgaca tcgaacttcg aacccatcac
     1081 cccgcgccaa tccagcgaag cgttgagcgt gccgaacgca tcgatcgtcg cgcccggttg
     1141 cacagccggc acgttcaccg cttcggtata ctggctcgcg gtgtgcgagt agttgacgaa
     1201 cagcgacagt gtcccggcat tgccggcaag cggctgatcc accgaggcat gcaggctcca
     1261 gatgtaggga gccgtgtact ggaagcgcag gcaagaggca tcgaccgtac cgcccggagg
     1321 aacgatgcca ttgcagccca gcccaccggt gccggtcacg aaggagtact tcgtatagtg
     1381 tgcgtcggtg tggctgaaat tgccgccgat ctccacgccc ggccacgggc ggatcgagcc
     1441 ttcgatctcg atgccgcgga tcttggcgtc ggcattgagc gtgcgagcac cgcccgcacc
     1501 cgtggtcggg ttatagtcgc cggtcgcgcg ctggatgtcg ttgtagtgga tctcatacag
     1561 cgaagtgttg aaccgcaccg ggacattgcc gagccggaaa tcggccttga accctgcttc
     1621 gtaggaaatc acgtgctcgg gcttgaaggt gcgcgtgtcg gggtctgtgc cctggccgaa
     1681 gaacacggca taggggttga acccgcccgc cttatagctg cggctcacct tggcgtagag
     1741 cagcaccggc ttcacgggcc gatagtccag gccgatcagc caagacggag agtccgactt
     1801 ctcggagcca tcgaacacac agctggccag ggcggagctt tccggcacga tcgagttgtc
     1861 cttgccgcag gccacgaagc cggcaggcag caaggggctg cgattgtact ggctggcagt
     1921 gccgtagacg tggtcccagg tatggcgata gccgccggtc aggcgcaggc tgtccagcgc
     1981 ggggctgaac gcgccgaaat cgagcgtcgc ctgcgcatag agcgcgttgg aaacggtcgt
     2041 aattccggaa atctgggtgc ttccgccgca gacgaaggcg ttgccggtga atgccgccgg
     2101 gcaatagaga atggcattgg agccctgcgg accggcgggg cgctggtcgt agtggaagcc
     2161 gccgaccgtc cagttcagct tgttgtccag catcttgccc tggaactgga gttcctctgt
     2221 ccagcccttc aggtcatcgc gcgggagttc gcgggcaagg gtcgcgttgc cgtaggtcgt
     2281 cgggccgagc ccgggcacgt cgaccgtacc ggagggcagc acgccgggat cgacttcgtg
     2341 ctgctgcaac gcggttgcat cgccgtcgta acggtagcgc aactgcatgc gctggaagct
     2401 ggcgatgttg cgcagggtga cgttgtcgct caactcgatg ttcgtcgtgt tggtcacgcc
     2461 ccaggtcttg gtgcgctgga actcgtcgac cgagaatgca gtcgcgcgcg gcccaagtgc
     2521 atcggcgcgc gcggtgttcg cgcggtagac atcgcacgaa gcgatcagga agggaattgt
     2581 cggcccttca tagcaggccg gggcgggcga accatctgcc gggttcacga tcgccttgag
     2641 cccgtcgatg ttgaagccct tatggatcag gccggccccg ttgttgtccg agtgggcgcc
     2701 gtagatcatc gtgtagttct cgatcccatc ggtcgggcgg aagagaatgc cgatgcgacc
     2761 ggagtaccag ttctcgttat cgcgatcctt gttccagatg acgtcgtggg tgtagccgtc
     2821 gcggcggtgg taggcgccaa ctacgcgcac catcagcttg tcttcgatca ccggcaggtt
     2881 gatcgcaccc tcgatatagg tgcggttgta gttgccgtac tcgcccttga tccagccgcc
     2941 cagttcgttg gtcggcttct tgggcacgag cagcacggcg ccgccggtgg tgttgcgccc
     3001 gaacagggtc ccctgcggac ccgagaggac ctgaaggttc tccagatcga cgaagttgcc
     3061 ggggccgccc tgctggctga gtgtcagtgc cgcaggcagc ggcacttcgt tgagatagac
     3121 gaccacaccc ggcgatgcct ggaaggtcgc accctggccg cggatggtga acgactgcga
     3181 atcgcgcgag ccctggccgt ttgccccgac cacgagcgag ggaacgctgg cctggagatc
     3241 ctgctccttg ttgatgccct gctgctcaag acgctgcggc gtcatggcgg tgatggcgat
     3301 cggcacgtcc tgcgcgcgct ctgcgcggcg gtttgccgta acgatgattt cgggagccgt
     3361 ggatgcgtcc gcggtgctcg aagcctgcgc ggcagccatc tcgggggcca tgatcgcaat
     3421 ggcggccacg ccccagttga cccatccgct ccgggcgcgc agccccttcg ctttcttgtt
     3481 catgtcagat ccttcctccc aggtgctgcc aagcgccggc gcgagttcgc accgcgcaaa
     3541 cggacgcatc cgcagcaacg catttcgact tatacgaagg gcaattcttc tactttcgcc
     3601 ctagcgacga gattaaatta cgcattatgg atgtgaatgc aaggggcagg tgccgagatg
     3661 cgtaaactgt tgcgcaaagg cttcagtccg ctggctttct gtttggttct gattattttt
     3721 gattgggtat gcacatcacg gtgcttgaaa aggaccaaaa atgtgcagcc tggcgctcga
     3781 taatcggggg agagaacgac tcgctccggc cgaccgcaag gcaatggttg cgtggataaa
     3841 tccgcccaat cggcgtcagc acgaaccggc cgtcaggaga tgcgtcaatc gctgcgaagg
     3901 atggccggga gcggacctgc cgcttcagtc cgccagcggg gccggaggtg accgcagctc
     3961 ggtcttcagg accttgcccg cagcattgcg gggcagctct ggcaggacga cgaaggagcg
     4021 gggaaccttg tagttggcca tgttctggcg cgaccaggca atgaggtcag cctccgacgg
     4081 gatttccgcc cccgggcgca ggaccacata ggccttgccc acctcaccca tgcgctcatc
     4141 ggccatgccg acgaccg
//
LOCUS       DATNBV010000381         2131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_236184,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000381 DATNBV010000000
VERSION     DATNBV010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236184"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VN627_09110"
     CDS             complement(<1..146)
                     /locus_tag="VN627_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXD03453.1"
                     /translation="MPENAAIEIEGLCKTYAGGKAALDNVSLSIPRGRIFGLLGPNGA
                     GKSTL"
     gene            complement(150..872)
                     /locus_tag="VN627_09115"
     CDS             complement(150..872)
                     /locus_tag="VN627_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HXD03454.1"
                     /translation="MKRLYTSASWEPDAATNARRILLDGKPVRTPARETLMVPGAALA
                     DAIIAEWNAQAERIAPATMPMTGFANATIDRVLPDAPGFGEGIAAYSASDLLCYRAAE
                     PPELVALQAAAWDPLLDWARARYDAAFAMTDSIMPVDQPEPTVARLHAAVGALDPWLF
                     AGLATIVTITGSLIGALALLEGAIEAGVLWDAAHVDEVWQARLWGEDADAVARTRIRR
                     AQFDDAARYCALVMRGSSALEA"
     gene            complement(869..1135)
                     /locus_tag="VN627_09120"
     CDS             complement(869..1135)
                     /locus_tag="VN627_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03455.1"
                     /translation="MTDPAMPPEPDAAAREKAARSRFLALSLFRLSGALILVFGLAIA
                     MQKFGWVTGQKARIMGVIIATVGFIQMVLVPRLLLRAWVTPPQP"
     gene            complement(1132..1791)
                     /locus_tag="VN627_09125"
     CDS             complement(1132..1791)
                     /locus_tag="VN627_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXD03456.1"
                     /translation="MTNRLAIFDCDGTLVDSQHNIVAAMDGAFIAAGLPPPTRAQTLS
                     IVGLSLVPAIAALIPEGDEALHVEVAEGYKTHFQTMRRSAGPAEPLFEGIADILDRLE
                     TDGWLLGIATGNSDRGLSHCLEAHGLLGRFVTVQTADRHPSKPHPSMIELAMAEAGAA
                     AGSTLMIGDTSFDMAMACAAGVRALGVGWGYHAPATLIEAGAESVAMDSAELGRHIGL
                     P"
     gene            complement(1788..>2131)
                     /locus_tag="VN627_09130"
     CDS             complement(1788..>2131)
                     /locus_tag="VN627_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HXD03457.1"
                     /translation="PAHFLETLADLGFDLDSGDLLLDEELPKTPKKLIEKQQAKAHSK
                     QMRKAHRGERQSRGGADGGPRASKPAAKAGVKPRPKPGTKPGTKPGTKPSPRAGSKPS
                     GPPRGRPPAKK"
BASE COUNT          304 a          712 c          753 g          362 t
ORIGIN      
        1 agcgtcgact tgcccgcgcc attggggccg agcaggccga agatgcgccc gcgcgggatg
       61 ctgagcgaga cattgtcgag cgcggccttg ccgccggcat aggtcttgca gagaccttcg
      121 atctcgatgg cggcattttc aggcatggct caggcttcta gcgcgctgct gccgcgcatt
      181 accagtgcgc aatagcgtgc cgcatcgtca aattgcgcgc gccggatcct ggtgcgggcg
      241 acggcgtcgg catcctcgcc ccacagccgt gcctgccaca cctcgtcgac atgggcagca
      301 tcccagagca ccccggcctc gatcgcccct tccagcaagg ccagcgcacc gatgagcgag
      361 ccggtgatgg tgacgatggt ggcgagacca gcgaacagcc aagggtcgag cgcgccgact
      421 gccgcgtgca gccgcgcgac cgtgggctcg ggctggtcga ccggcatgat gctatccgtc
      481 atggcaaagg cggcatcata gcgtgcgcgg gcccaatcga gcagcgggtc ccatgccgcc
      541 gcttgcaggg cgaccagctc ggggggttcg gcggcgcggt agcagagcag gtcgctcgcg
      601 ctgtaggccg cgatgccctc gccaaatccg ggggcatcgg gcagcacccg gtcgatcgtg
      661 gcattggcaa agccggtcat cggcatggtc gccggggcga tccgctcggc ctgcgcgttc
      721 cattcggcaa tgatcgcgtc ggccagcgcg gcgcccggaa ccatcaatgt ctcgcgggcg
      781 ggcgtgcgca ccggcttgcc gtcgagcagg atgcgccgcg cgttcgtcgc ggcgtccggc
      841 tcccagcttg cgcttgtgta gaggcgtttc atggctgcgg cggggtgacc caggcgcgca
      901 gcagcaggcg cgggaccagc accatctgga taaagccgac ggtggcgatg atcacgccca
      961 tgatcctggc cttctgcccc gtcacccagc cgaatttctg catcgcgatg gcgaggccaa
     1021 agacgaggat cagcgcgccc gacaggcgga acaggctgag cgcgaggaag cggcttcgcg
     1081 ccgccttttc gcgggcggcg gcatcgggtt cgggcggcat ggcggggtcg gtcatggcag
     1141 gccgatatgg cggccaagtt ccgcgctgtc catggcgacg ctctcagccc ccgcctcgat
     1201 cagcgtcgcg ggcgcatgat agccccagcc gacgccaagc gcgcgcacgc cggccgcaca
     1261 ggccatcgcc atgtcgaaac tggtgtcgcc gatcatcaac gtgctgccgg cggcggcgcc
     1321 cgcttcggcc atggcgagtt cgatcatgct cggatgcggc ttgctggggt gccggtcggc
     1381 ggtctggacc gtgacgaacc ggccgagcag gccgtgcgcc tccaggcaat ggctcaggcc
     1441 cctgtcggaa ttgccggtcg cgatgccgag cagccagccg tccgtctcca gccggtcgag
     1501 tatgtcggcg atcccctcga acagcggttc ggccggtccg gccgagcgtc gcatcgtctg
     1561 aaaatgcgtc ttatatccct ccgcgacctc gacatgcaaa gcctcgtcgc cctccgggat
     1621 cagcgccgcg atggccggga ccagcgacag gccgacgatg gagagggtct gcgcccgcgt
     1681 cggtggcggc agacctgcgg cgataaacgc cccgtccatc gccgcgacaa tattatgctg
     1741 gctgtcgacc agcgtgccgt cgcagtcgaa tatcgcaagg cggttggtca ttttttggcc
     1801 ggcggccgtc cgcgcggggg gccgctcggc ttggagccgg cccttgggct gggcttggtt
     1861 ccgggctttg ttccaggctt tgttccgggc ttcggccggg gctttacgcc ggctttcgcg
     1921 gcgggcttgc ttgcgcgcgg gccgccgtcg gcgccgcccc gtgattgccg ctcgccccgg
     1981 tgcgctttgc gcatctgctt ggaatgggcc ttggcctgct gcttctcgat cagctttttc
     2041 ggggtcttgg gcagttcctc gtcgagcagc aggtcgccgg aatcaaggtc gaagccgagg
     2101 tccgccagcg tttcgaggaa atgcgcgggc g
//
LOCUS       DATNBV010000382         2455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3424402,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000382 DATNBV010000000
VERSION     DATNBV010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3424402"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VN627_09135"
     CDS             <1..842
                     /locus_tag="VN627_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HXD03458.1"
                     /translation="VNSQRGGPSTGLPTKTEQSDLYQAVYGRNGDAPLPVIAARSPAD
                     AFDCAIEACRIAVQFMTPVILLTDGYIANAAEPWAVPDPASYKPFPVEFLAENNNPEG
                     GKVLPYKRDAQGVRPWIKPGTPGLMHRIGGIEKAPDTGDLDYSPAVHQLQTDARKSKI
                     DGVAKAIPAQGVELGNTTGKLAVVGWGSTYGPIIQAVRRARAKGQDVSHIHVRHIWPM
                     PENLGDLLRGFDKVLVPEMNTGQFKTVLRDQFLIDAKPLNKTSGQPYTIAEIEAAIDE
                     MLA"
     gene            854..1900
                     /locus_tag="VN627_09140"
     CDS             854..1900
                     /locus_tag="VN627_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HXD03459.1"
                     /translation="MNDMTPIQTTLKDWESDQEVRWCPGCGDYAILKAVQRTLPEIGA
                     DPAKTVFVSGIGCSSRFPYYVESYGFHTIHGRAPAFATGIKLANPELDIWLVTGDGDG
                     MSIGGNHTMHLLRRNLNCQIMLFNNEIYGLTKGQYSPTSREGTSSPSTPMGSVDHPAL
                     PCAFALGSGARFVARGFDVSKELTGVLKAAHAHRGAAFVEIFQNCIVYNKDRFEDFAA
                     PKGAEANQLWLKDGEPMLFGSEKSGGVKGITLDMATLSLKVVDVVDGDWQAAGVIVHN
                     VKNKAVAHMLVEMPFGPFPMALGVIFDDPRPTYEDAVHGQDAKAREGRVADLGKLLAK
                     GQTWTVSGTAADPI"
     gene            1963..>2455
                     /locus_tag="VN627_09145"
     CDS             1963..>2455
                     /locus_tag="VN627_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HXD03460.1"
                     /translation="MTSPVIVWLRRDLRLADQAAFHAATESGAPVIPVYVLDDEAPRP
                     HRMGGASRWWLHHSLAALGADLGAIGSRLILRRGKVHEELAAIRRDVGARTIHCLAHY
                     EPWWRNAEKAVAKHFQLVRHDGNYLAPPGTILSGSGTPYKIYTPFWRALRERLPPAEP
                     LPAP"
BASE COUNT          433 a          856 c          782 g          384 t
ORIGIN      
        1 tcgtcaactc gcagcgcggc gggccctcca ccggcctgcc gaccaagacc gagcagtctg
       61 acctctatca ggcggtctat ggccgcaacg gcgatgcccc gctgccggtg atcgccgcgc
      121 gcagccccgc cgatgcgttt gactgcgcga tcgaggcctg ccgcatcgcg gtccagttca
      181 tgaccccggt gatcctgctg accgacggct acatcgccaa tgcggccgag ccctgggccg
      241 tgccagatcc ggccagctac aagccgttcc cggtcgaatt cctcgctgaa aacaacaatc
      301 ccgagggcgg caaggtcctg ccctacaagc gcgatgcgca gggcgttcgt ccctggatca
      361 agccgggcac gccgggcctg atgcaccgca tcggcggcat cgagaaggcg ccggacacgg
      421 gcgaccttga ttacagcccc gccgtccacc agctgcagac cgatgcccgc aagagcaaga
      481 ttgacggcgt ggccaaggcg atcccggcgc agggcgtgga actgggcaac accacgggca
      541 agctcgctgt ggtcggctgg ggttcgacct acggcccgat catccaggct gtccgccgcg
      601 cgcgggccaa ggggcaggac gtcagccaca tccacgtgcg ccacatctgg ccgatgccgg
      661 agaacctggg cgacctgctg cgcgggttcg acaaggttct ggtgcccgag atgaataccg
      721 gccagttcaa gaccgtgctg cgcgaccagt tcctgatcga cgccaagccg ctcaacaaga
      781 ccagcggcca gccctacacc atcgcggaaa tcgaagccgc gatcgatgaa atgctggcct
      841 gagggaattc gacatgaacg acatgacccc catccagacc accctgaagg actgggaatc
      901 ggaccaggaa gtccgctggt gccccggttg cggtgactat gcgatcctca aggcggtgca
      961 gcgcaccctg ccggagatcg gcgccgatcc ggccaagacc gtgttcgtga gcggcatcgg
     1021 ctgctccagc cgcttcccct actatgtgga aagctatggc ttccacacga tccacggccg
     1081 cgccccggcc ttcgccaccg gcatcaagct cgccaacccc gagctcgata tctggctggt
     1141 gaccggcgat ggcgacggca tgtcgatcgg cggaaaccac acaatgcacc tcctgcggcg
     1201 caatctgaac tgccagatca tgctgttcaa caacgagatc tacggcctga ccaagggcca
     1261 gtactcgccc accagccgtg agggcacgtc cagcccgtcg accccgatgg gttcggttga
     1321 ccatcccgcg ctgccctgtg ccttcgcgct cggttcgggc gcgcggttcg tcgcgcgtgg
     1381 gttcgacgtg tccaaggagc tgaccggcgt gctcaaggcc gcccatgccc accgcggcgc
     1441 ggcgttcgtc gagatcttcc agaactgcat cgtctacaac aaggaccgtt tcgaggactt
     1501 cgccgccccc aagggcgccg aggccaacca gctgtggctc aaggatggcg aaccgatgct
     1561 gttcggctcg gagaagtcgg gcggggtcaa gggcatcacc cttgatatgg caacgctttc
     1621 gctcaaggtt gtcgacgtgg ttgatggcga ctggcaggcg gccggcgtga tcgttcacaa
     1681 cgtcaagaac aaggccgtgg cccacatgct ggtggaaatg cccttcgggc cgttcccgat
     1741 ggcgctcggc gtgatcttcg acgatccgcg cccgacctac gaggacgctg tccatggcca
     1801 ggatgccaag gcgcgcgaag gccgggtggc cgatctcggc aagctcctcg ccaagggcca
     1861 gacctggacc gtgagcggca cggcagccga tccgatctga cgggcacgga cctgccctaa
     1921 ggggcaggtc tgtcccgctc cttcatccgc tagcctcagg cgatgaccag tcccgtcatc
     1981 gtctggctgc gccgcgacct gcgccttgct gatcaggccg cattccacgc agcgacggaa
     2041 tccggcgcgc cggtaatccc cgtctatgtg ctcgatgacg aggcgcccag gccccaccgg
     2101 atgggcggcg cctcccgttg gtggctgcac cactcgctcg ccgcgctcgg ggccgacctc
     2161 ggggctatcg gctcacgcct gatcctgcgg cggggcaagg tgcacgagga actggcggcg
     2221 atccgccgcg acgtgggcgc gcggacgatc cattgcctcg cccattacga gccctggtgg
     2281 cgcaatgctg aaaaggccgt ggccaagcat ttccagctgg tgcgccacga tggcaactat
     2341 ctggcgccgc ccggcacgat cctgagcgga tcgggcacgc cctacaagat ctacacgccg
     2401 ttctggcggg cgctgcgcga acgcctgccg ccggccgaac cgctgccggc cccgg
//
LOCUS       DATNBV010000383         7152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4693789,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000383 DATNBV010000000
VERSION     DATNBV010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4693789"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VN627_09150"
     CDS             complement(<1..253)
                     /locus_tag="VN627_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03461.1"
                     /translation="MPPEIEKPPARPPSDEFASAPAPDAVNREQEDESTQAQTVADEV
                     LARAEGAGQFGLDETEKVKAGDETDDVQDLVDHMRQMETS"
     gene            complement(311..892)
                     /locus_tag="VN627_09155"
     CDS             complement(311..892)
                     /locus_tag="VN627_09155"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXD03462.1"
                     /translation="MVKLNKIYTRTGDDGTTGLVDGSRCPKHAARMEAIGAVDEANSA
                     IGLATLASMSDEHRALLLRTQNDLFDLGADLATPTSSGGDDFAPSEMVLRMVTGQTAY
                     LEQAIDRLNEHLAPLTSFILPGGSEAAARVHIARAAVRRAERRMVALAAEAPVNPAAL
                     SYVNRLSDLLFVLARALNGGGAGDVLWTPGANR"
     gene            complement(897..1130)
                     /locus_tag="VN627_09160"
     CDS             complement(897..1130)
                     /locus_tag="VN627_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03463.1"
                     /translation="MTYLLVPIIVILMIMVVVSLVRGIMAFLKSTRTDLERGDAPGAT
                     EMQLLQNKMMFNRIKYQAAAILVVALLLAVAKS"
     gene            complement(1127..1972)
                     /gene="gluQRS"
                     /locus_tag="VN627_09165"
     CDS             complement(1127..1972)
                     /gene="gluQRS"
                     /locus_tag="VN627_09165"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HXD03464.1"
                     /translation="MIVTRFAPSPNGPLHLGHAYAAVVAHDLARARGGRFLLRIEDID
                     GERSRAELAGEFEADLAWLGLAWDGPTLHQSDRLAAYAHAAQRLKAMGLLYPCQCTRA
                     EVAEAATQMGPDGPVYPGTCRHRPADPTGAAWRLDVAKALALTGPLSWTDELAGEQEA
                     RPGLLGDVVLLRKDAPASYHLAATLDDAADGITLVTRGADLFAASHVHRLLQALLGLP
                     VPTWHHHGLLVEPDGKKLAKRRNAPSLADRRRAVQDGPALADALRAHRFPAGISLASS
                     LHGEP"
     gene            2117..2725
                     /locus_tag="VN627_09170"
     CDS             2117..2725
                     /locus_tag="VN627_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXD03465.1"
                     /translation="MLKSELIERAARFRSAEDDPSRHLANCPALVLNADYTPLSYYPL
                     SLWPWQTAIKAVFLERVDIVASYEREVHSANWTMQIPSVIALRQFVKPGQFPAFTRFN
                     VFLRDRFSCQYCGSPHSLTFDHVVPRRLGGRTSWENVATACAPCNLKKGGRTPRQAKM
                     PLLIEPIRPTSWQLQERGRGFPPNYLHETWRDWLYWDVELEA"
     gene            2834..3673
                     /locus_tag="VN627_09175"
     CDS             2834..3673
                     /locus_tag="VN627_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HXD03466.1"
                     /translation="MTASPWSHARNPALADDIEFEIKGQELQFVEIELDPGESAVAEA
                     GAMVWKDGAVGMTTVFGDGRGAEAGFMGKLLGAGKRLVTGESLFTTVFTHNGSGKARV
                     AFAAPVPGAILPMKLDELGGTLICQKDAFLCAAKGVSIGVHFQRRVMTGLFGGEGFIM
                     QRLDGDGWVFVQMGGTVVERELAAGEELHVDTGCLAAYTPSVEFDLVSAGNVKTMLFG
                     GEGVFFARLTGPGKVWIQSLPFARLAGRVIATAMPNLGPMGGQNRGEGSLLGGLGNLI
                     DGN"
     gene            complement(3714..4364)
                     /locus_tag="VN627_09180"
     CDS             complement(3714..4364)
                     /locus_tag="VN627_09180"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXD03467.1"
                     /translation="MTYSRTIRPANRLGTLAAVGALHVAAFYAVISGLAVVFVPHDDP
                     PPFSAINHPLPKLPPVHKPPSHHARQPLPDPLPQPTQTTEVFIPLGPAGGPAAGGDTR
                     PTDPPLPPLRPNPPLFTPKAARPLGQPGTWVGESDYPTGELRLGHAGAVGFSLTIGSA
                     GQVTGCTVTRSSGYRALDEATCRLLARRARFEPARDEQGLPAAGAYASTVRWQIPD"
     gene            complement(4509..4970)
                     /locus_tag="VN627_09185"
     CDS             complement(4509..4970)
                     /locus_tag="VN627_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03468.1"
                     /translation="MPFPLPPAPGPAPLAAAPAVLAAGVGLASVTFGLGSARDAFAAG
                     GAAAPAGLGTGFGGGVGLGSGVTSISVTVIAGSAGLPSQCARNPLISTQARPASIASA
                     AAIAASRTGVHLRLPPLLLAMACLSLRERSKLGFAPETLLPSPEPSNTAWQ"
     gene            5014..5286
                     /locus_tag="VN627_09190"
     CDS             5014..5286
                     /locus_tag="VN627_09190"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXD03469.1"
                     /translation="MPAQRVGGALSDRDYPRDAGRAGGTVGIAFTVRSDGRVDGCRVL
                     GSSGSARLDGLTCSLVTQRFRYRPARDAGGQAVDSTLRTAFTWGTR"
     gene            5420..6025
                     /gene="hisB"
                     /locus_tag="VN627_09195"
     CDS             5420..6025
                     /gene="hisB"
                     /locus_tag="VN627_09195"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HXD03470.1"
                     /translation="MRTARIARKTHETEIEVEVNLDGTGAYQVSTGIGFLDHMIEQFS
                     RHSLIDITCRIVGDLHVDQHHTTEDSAIAIGQCIAQALGDKAGIGRYGEAHSAMDEAL
                     LRVVLDISGRPWLVWNAKFTQERLGEMDTELFEHWFHSIAQAVGITLHIEQIYGSNNH
                     HVIEGIYKGFARAMRQAVELDPRKGGAVPSTKGVLGEGSLS"
     gene            6026..6655
                     /gene="hisH"
                     /locus_tag="VN627_09200"
     CDS             6026..6655
                     /gene="hisH"
                     /locus_tag="VN627_09200"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HXD03471.1"
                     /translation="MAETLALIDYGAGNLHSVENALKAAGAAGVAVTADPDVVRAADR
                     IVLPGVGSFKACAEGLRAIPGLVAAMEERVRVGGAPFLGICVGMQLLATRGVEHGVTE
                     GLGWIGGEVRLIEPSDPAIKIPHMGWNDVALTPHSDGAGLIEPGEAYFLHSYQFLPDD
                     GRAVAAMTDHGGGIVAAVAEDNVVGVQFHPEKSQAYGLALLARFLEWKP"
     gene            6792..>7152
                     /locus_tag="VN627_09205"
     CDS             6792..>7152
                     /locus_tag="VN627_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HXD03472.1"
                     /translation="MIVFPAIDLKGGQVVRLSEGDMARATVYGDNPAAQAQLFAEAGS
                     QYLHVVDLDGSFAGRAENREAVESILAAFDGHVQLGGGIRTREAVEGWFDLGVSRVVM
                     GSAALKDPEFVKDMAREF"
BASE COUNT         1089 a         2147 c         2755 g         1161 t
ORIGIN      
        1 cgctggtttc catctggcgc atgtgatcga cgaggtcctg gacgtcgtcg gtctcgtcgc
       61 cggccttgac cttttcggtc tcgtccagac cgaattgccc ggcgccttcg gcccgggcga
      121 gcacttcgtc ggcaacggtc tgggcctggg tgctttcgtc ttcctgctcg cggttgacgg
      181 catcgggcgc gggggcactg gcgaattcat cgctgggcgg gcgggcgggt ggtttctcga
      241 tctcgggggg catggcaaat cctttcgggt ttgcccgatc aagccttgcg cgtgggggaa
      301 ggttcccgcc ttagcgattg gcccccgggg tccacagcac gtcgcccgcc ccgccgccgt
      361 tcagcgcgcg ggcgaggacg aagagcaggt cggacaggcg gttgacgtag ctcagcgccg
      421 ccggattgac cggggcctcg gcggcgaggg cgaccatgcg gcgttcagcc cggcgcacgg
      481 cggcgcgggc gatgtggacg cgggccgcgg cctcgctgcc gcccggcagg atgaagctgg
      541 tcagcggggc gaggtgctcg ttgaggcgat cgatcgcctg ttcgaggtag gcggtttgcc
      601 ccgtgaccat gcgcaggacc atctcggacg gggcgaaatc gtcgccgcca ctggacgtgg
      661 gcgtggcaag atcggcgcca aggtcgaaca ggtcgttctg ggtgcgcagg agcagggcgc
      721 gatgctcgtc cgacatcgag gcgagggtgg cgagaccgat ggcgctgttg gcctcgtcga
      781 ccgcgccgat cgcttccatc cgcgcggcgt gcttggggca gcgcgaaccg tcgaccaggc
      841 cggtggtgcc gtcgtcgccg gtgcgggtgt agatcttgtt gagcttgacc aagaacttac
      901 gacttcgcga ccgcgagcag cagggcgacc acgaggatcg cggcggcctg gtacttgatg
      961 cggttgaaca tcatcttgtt ctggaggagc tgcatttcgg ttgcccccgg cgcgtcgccg
     1021 cgttcgaggt cggtccgggt gctcttgagg aaggccatga tgccgcgcac caggctgacg
     1081 acgaccatga tcatcaggat gacgatgatg ggcacgagaa ggtaggtcat ggttcaccat
     1141 gtaggcttga agcgaggctt atgccagcag ggaagcgatg ggcgcgcaag gcgtctgcaa
     1201 gggcagggcc gtcctgcacg gcgcggcggc ggtcggccag ggacggggcg ttgcggcgtt
     1261 tcgccagctt cttgccatcg ggttcgacca gcaggccatg gtggtgccag gtgggcacgg
     1321 gcaggccgag cagggcctgg agcaggcggt ggacatggct ggcggcgaac aggtcggcgc
     1381 cgcgggtgac gagggtgatc ccgtcggccg cgtcgtcgag cgtggcggcg aggtggtagc
     1441 ttgccggggc gtccttgcgc aggaggacga cgtcgcccag caggccgggc cgggcctctt
     1501 gctcgccggc cagttcgtcg gtccaggaga gcgggccggt cagggcgagg gccttggcca
     1561 cgtcgagccg ccaggcggcg cctgtcggat cggccgggcg gtggcggcag gtgccggggt
     1621 agaccgggcc atcggggccc atttgcgtgg cggcctcggc cacttcggcg cgggtgcact
     1681 ggcaggggta gagcaggccc atggccttga ggcgctgggc ggcatgcgca taggcggcca
     1741 gccggtcgga ctggtgcagg gtggggccat cccaggcgag gccgagccag gcgagatcgg
     1801 cttcgaactc gccagccagt tcggcccggc tgcgttcgcc gtcaatatcc tcgatccgca
     1861 ggagaaagcg gccgccgcgc gcccgggcga gatcgtgcgc gaccacggcg gcataggcgt
     1921 ggccgaggtg cagcgggccg ttggggctgg gggcgaagcg ggtgacgatc atgccatgcc
     1981 tgtcatagcg gcgcggcggg cgctttccag ttgtggatag tgccgtcaat gccgccttga
     2041 ccgggagcgc ggcaagtgct ggattccgcg tcatggcttg tcgcaaatcc gcaacgcggg
     2101 cgtttcacaa gaggtcatgc tcaagagcga gctgatcgag cgcgcggccc ggttccgcag
     2161 cgcggaggat gatccctccc ggcacctggc caattgccct gcgctggtgc tgaacgctga
     2221 ctacaccccg ctttcctact atccgctgag cctgtggccg tggcagacgg cgatcaaggc
     2281 ggtgttcctg gagcgggtgg acatcgtcgc gagctatgag cgcgaggtcc attcggccaa
     2341 ctggacgatg cagatcccct cggtgatcgc gctcaggcag ttcgtgaaac ccgggcagtt
     2401 tccggccttc acgcggttca acgtgttcct gcgggaccgg ttttcctgcc agtattgcgg
     2461 cagcccgcac agcctgacct tcgatcacgt ggtgcctcgg cgcctcggcg ggcggaccag
     2521 ctgggagaac gtggccaccg cctgcgcgcc gtgcaacctc aagaagggcg ggcgtacgcc
     2581 gcggcaggcg aaaatgccgc tgctgatcga accgatccgg ccgacgagct ggcaattgca
     2641 ggagcgcggg cgcggtttcc cgccgaatta cctgcatgag acctggcgcg actggctcta
     2701 ctgggatgtg gagctggaag cctgagcttg ggccgtgaat tggcccaggg agggctgcca
     2761 acggcacatt ctcgctccac cctgagccaa ttcgcggacc aagctccctc acccgaaacc
     2821 tatggagaat tgaatgaccg ccagcccctg gagccacgcg cgcaatcccg cgctggccga
     2881 tgatatcgag ttcgagatca agggacagga actgcagttc gtcgagatcg agctcgatcc
     2941 aggcgagagc gccgtggccg aagcgggggc catggtgtgg aaggacgggg cggtggggat
     3001 gaccaccgtg ttcggcgacg ggcgcggggc ggaagccggg ttcatgggca agctgcttgg
     3061 cgcgggcaag cggctggtga cgggggagag cctgttcacc accgtgttca cccacaacgg
     3121 atcgggcaag gcgcgggtgg cctttgcggc gccggtgccc ggggcgatcc tgccgatgaa
     3181 gcttgacgag ctgggcggga cgctgatctg ccagaaggat gccttcctct gcgcggccaa
     3241 gggagtttcc atcggcgtcc acttccagcg ccgggtgatg accgggctat tcggcggcga
     3301 aggcttcatc atgcagcggc tggacggcga tggctgggtt ttcgtccaga tgggcggcac
     3361 tgtggtcgag cgcgagctgg cggcgggcga ggagctgcac gtcgataccg ggtgcctcgc
     3421 cgcctatacc ccttcggtgg agttcgacct cgtcagcgcg gggaatgtta agaccatgct
     3481 gtttggcggc gagggcgtgt tcttcgcgcg gctgaccggg ccgggcaagg tgtggatcca
     3541 gtcgctgccc tttgctcggc tggccgggcg ggtgatcgcc acggctatgc cgaatcttgg
     3601 gccgatgggc ggccagaacc gtggcgaggg ctcgctgctg ggcgggctgg gcaacctgat
     3661 cgacgggaac tgagccgggc cccaccggag ttctgccggc agggcccggc cggtcagtcg
     3721 gggatctgcc agcggacggt gctggcatag gcaccggcgg cgggcaggcc ctgttcgtcg
     3781 cgcgcgggct cgaagcgggc acggcgggca aggaggcggc aggtcgcttc gtcaagcgcg
     3841 cgatagccgc tggagcgggt gacggtgcag ccggtcacct gcccggcgct gccgatggtg
     3901 aggctgaacc cgaccgcgcc ggcatggccg aggcgcaatt cgccggtcgg atagtcgctc
     3961 tcgcccaccc aggtgccggg ctggccgagc gggcgggccg ccttgggggt gaatagcggc
     4021 gggttcggcc ggagcggggg gagcggcgga tcggtggggc gggtgtcgcc gccggccgcc
     4081 ggcccaccag cgggcccgag tggaatgaac acttcggtgg tctgcgtggg ctgcggcagc
     4141 ggatcgggga gcggctggcg ggcatggtgg ctgggcggct tgtgcaccgg gggcagcttg
     4201 ggcagcggat ggttgatcgc gctgaacggc gggggatcgt cgtgcggcac gaagacaacg
     4261 gcgagccccg agatgacggc atagaaggcc gccacgtgca gggcgccgac cgcggcaaga
     4321 gtgccgaggc gattggccgg acgaatggtg cgactgtagg tcatggacac acctctctta
     4381 cagttacggc gaacccgggt tcgtcgctgt tatggtggtt gtccggactg ttcttgccta
     4441 tgccatgaaa agtagctcgc gacaactcta aattacttat aagagcaaca atattataag
     4501 cacggcagtc attgccatgc tgtattcgac ggttctggcg agggaagcag cgtttcggga
     4561 gcgaagccga gcttgctgcg ttcgcgaagc gagaggcagg ccatggcgag caagagcgga
     4621 ggcaggcgca ggtggacgcc ggtgcggctg gcggcgatgg cggcggcgct ggcgatcgac
     4681 gcgggccttg cctgggtgct gatcaacgga ttgcgcgcgc attggctggg gagcccggcc
     4741 gaaccggcga tgaccgtgac cgagatcgag gtgacccccg agccaagccc cactccgccg
     4801 ccgaaacccg tacccaagcc cgccggggcg gccgctcccc ccgccgcgaa ggcgtcgcgg
     4861 gcggagccga gcccgaaggt gacgctggcc agcccgaccc cggcggcgag cacggccggg
     4921 gcggcggcga gcggggcagg accgggggca ggaggcagcg ggaatggcac gggtgcgggc
     4981 agtggcggca acggcggcgg cgggggggcg gccgtgccgg cgcagcgggt gggcggagcc
     5041 ctgtccgacc gcgattatcc gcgcgatgcg ggccgggccg gggggacggt ggggattgcc
     5101 ttcactgtcc gcagcgatgg ccgggtggat ggctgccggg tgcttggttc gagcgggtcg
     5161 gcgcggctcg acgggctgac ctgcagcctg gtgacccagc ggttccgcta tcgcccggcg
     5221 cgcgatgccg gcgggcaggc ggtggattcg acgcttcgga ccgcgtttac ctgggggacg
     5281 cggtgaggtc cttcgagacg ccgttcagcc tgcggcttcc ggctcctcgg gacgagcggt
     5341 gatccatggg tggccttgcc cccgcttgca ccttgggccc ttgggtctat aggggcgcca
     5401 gcccaaggaa tcccgaccga tgcgcaccgc ccggattgcc cgcaagaccc atgagaccga
     5461 gatcgaggtg gaagtgaacc tcgacggcac cggtgcctac caggtgtcga ccggaatcgg
     5521 ttttctcgat cacatgatcg agcagttcag ccggcattcg ctgatcgaca tcacctgcag
     5581 gatcgtgggc gacctccatg tcgaccagca ccacacgacc gaggattcgg ccatcgcgat
     5641 cggccagtgc atcgcccagg cgctgggcga caaggccggg atcggccgct acggcgaggc
     5701 ccattcggcg atggacgagg cgctgctgcg cgtcgtgctg gatatctccg gccggccatg
     5761 gctggtgtgg aacgcgaagt tcacgcagga acggctgggc gaaatggaca ccgagctgtt
     5821 cgagcactgg ttccattcga tcgcccaggc cgtgggaatc acgctgcaca tcgagcagat
     5881 ctacggcagc aacaatcacc acgtgatcga gggcatctac aagggctttg cccgggcgat
     5941 gcgccaggcg gtggaactgg acccgcgcaa aggcggggcc gtgccgagca ccaagggggt
     6001 gctgggagag ggatcgctgt cgtaaatggc tgagacgctg gccctcattg attacggcgc
     6061 gggcaatctc cactcggtcg agaacgcgct gaaggcggca ggagccgccg gcgtggccgt
     6121 gaccgccgac ccggacgtgg tgcgcgcggc agaccggatc gtgctgccgg gcgtgggcag
     6181 cttcaaggcc tgtgccgaag ggctgcgggc gattcccggg ctggtcgccg ccatggaaga
     6241 gcgggtgcgg gtcggcggtg cgccgttcct cggtatttgc gtaggcatgc aattgctggc
     6301 gacgcgcggc gtggagcatg gcgtgaccga ggggctcggc tggatcggcg gcgaggtccg
     6361 gctgatcgag ccttccgatc cggcgatcaa gattccccac atgggctgga acgacgtggc
     6421 cctgacgccg cattccgacg gggccgggct gatcgagccg ggcgaggcct attttctcca
     6481 ctcataccag ttcctgcccg atgatggccg ggccgtggcg gcgatgaccg accatggtgg
     6541 ggggatcgtt gccgccgtgg ccgaggacaa tgtggtgggc gtgcagttcc acccggagaa
     6601 gagccaggcc tatgggctgg ctctgctcgc aagatttctg gaatggaagc cgtgagggcg
     6661 gcaaacccct ctccaggctt cgctagccgc cggggcggca agctgcgcca tcctctcccc
     6721 cgccggggga gaggatacga aggcctggcg acgcaggagc ctggccgcag ttggagaggg
     6781 gcatcccacg catgatcgtt tttcccgcca tcgacctcaa gggcggccag gtcgtgcgcc
     6841 tgtcggaagg cgatatggcg cgcgcgaccg tttatggcga caatccggcg gcccaggcgc
     6901 agctgtttgc cgaggcgggt tcgcagtatc tccatgtggt ggacctcgac ggttcgtttg
     6961 ccgggcgggc ggagaaccgg gaggcggtgg aatccattct cgccgcgttt gacggccatg
     7021 tgcagctggg cggcggcatc cgcacgcgcg aggcggtgga gggctggttc gaccttggcg
     7081 tttcgcgggt cgttatgggt tcggcggcgc tgaaggaccc ggaattcgtc aaagacatgg
     7141 cgagggagtt tc
//
LOCUS       DATNBV010000384         6771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1804580,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000384 DATNBV010000000
VERSION     DATNBV010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804580"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VN627_09210"
     CDS             <1..445
                     /locus_tag="VN627_09210"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HXD03473.1"
                     /translation="LNHKVIFIVTNAIDPAWTSGYAERFDYRVDVLRRRNLVEAATPD
                     GRQVARSERTLLIDEQSHGLVFYFPRSDVDMSALVRIDRVSHCPFKGVASYFALAGNA
                     AGEPVAWSYETPYPEVSQIAEHIAFYQDRMVVRLGVAIFPIPKKA"
     gene            455..1351
                     /locus_tag="VN627_09215"
     CDS             455..1351
                     /locus_tag="VN627_09215"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HXD03474.1"
                     /translation="MANPTSNDTYEQHWGDLADLARIIDLQPTAPGVFSAPIYEGAPR
                     NVVEGSQMLAQSIVAAAKQMPEKRVISAYAQFARVAGFDRPLDFHVDVLQSGRTFASL
                     SVKAEQDGKLRCPAMVLMDAGSPDFIAHQVPMPQVPGPDECPDYDYGMTGRDVRFVNG
                     DYTPHEHRMGDPVLHCWMRCRDKPKTQLMHQALLTQPVGHFTIGAAMLPHPGVKEGDA
                     HVTLSTGIMSIAIAYHADADVSGWVLYSNPAIWSGRGLVQGHGTVFAQDGTLLASYTV
                     TGMVREFAPDAAIAGIAANQLM"
     gene            1447..2217
                     /locus_tag="VN627_09220"
     CDS             1447..2217
                     /locus_tag="VN627_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD03475.1"
                     /translation="MDFGLAGKVAVVAGGSRGCGLAIARELAREGASVVLTGRNRDIV
                     EAAAEAIRADGGAAYGLTTEMVSAEDAARIAQAARDKFGDPGILVVNPPTPGNAAGFF
                     EVTDEDYLRAHEYFNMPLVRLSREMIPAMQRGQWGRIVNMASVGIKQPHLKFLLYATN
                     TRVAAAAIAKTMSFEFAKDNITVNTIATGPFPSALADEYNKDAGYAADRIAKSTPMKR
                     LGRTEEMAAMVSFLCSDRASFVSGETIRVDGGSTFNLF"
     gene            2263..3075
                     /locus_tag="VN627_09225"
     CDS             2263..3075
                     /locus_tag="VN627_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03476.1"
                     /translation="MELNLAGKRALVTGSTAGIGEAIAKTLAREGAKVVINGRSERRG
                     KAVAEAIEAAGGEALLALGNVASDEDADRVGREVRDAWGGVDILVNNAAGFASGSSVG
                     SFFTVTAEEWVKTYDLNVGAAVRMMNLLVPDMAERGWGRVINIGSSGGWMPTGELPDY
                     SVAKIAMMGLTANAARGLSGTGVTVNSVVPGVIYTRSVAAWFRAIGEQQGWGDDRAKS
                     EAYVLARHGQTVNRVGEVADIANMVAYLASPLSGYINAVNLRIDGGAFSAVG"
     gene            3204..4151
                     /gene="egtD"
                     /locus_tag="VN627_09230"
     CDS             3204..4151
                     /gene="egtD"
                     /locus_tag="VN627_09230"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HXD03477.1"
                     /translation="MVTAPADPAFLAAVTTGLQRPVKAIPPRFFYDLEGSHLFDEITR
                     LPEYYPTRTETALLRRHAEDLAAAVGTGRPVIEFGAGSATKTPLLLDALATQTYVPID
                     ISGDYLEQAVAQLGQARPGLRIVPVVGDFTTPLALPPLSGPVTGFFPGSTIGNFDPAE
                     AIGLLRSFRQLLGEGGRLVIGIDTRKDRRVLEAAYDDAAGVTAAFNRNLLTRINRELA
                     GTIPVEHFAHRAIWNEAEGRVEMHLEALRDLRFTVAGEAFAMAGGETIHTESCYKYTP
                     AEADRLARAGGWEPLVCWTDPEDLFGLHVWGAVPDARLP"
     gene            4148..5350
                     /gene="egtB"
                     /locus_tag="VN627_09235"
     CDS             4148..5350
                     /gene="egtB"
                     /locus_tag="VN627_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HXD03478.1"
                     /translation="MSAGRAERYNEVRAATEALTSGLSAEDCQVQSMPDVSPAKWHLA
                     HTTWFFETFLLLPMLEGYRPLDPAFAVLFNSYYVGVGERHPRPSRGLLTRPSLDEVHA
                     YRRHVDAAMRRLMETVAEPAWAWLVELGCQHEQQHQELILMDIQHVLSCNPLEPAYRR
                     PDPAGEPSGPLAWSDHPGGLCEIGHGGDGFAFDNEGPRHRVWVEPFAVANRLVTAGEY
                     LGFIEDGGYRRPELWLSDGWTTVEAQGWQAPLYWDKSGGQWTRFTLAGRRQVTLSEPV
                     CHVSYYEAEAYARWAGARLPSEAEWELAAADPAMVQLYDTAWQWTASPYVGYPGYVPL
                     KGAVGEYNGKFMINQQVLRGGSLATPAGHSRATYRNFFPPAARWLFGGIRLAKGWNGA
                     APRQAAPD"
     gene            5381..5875
                     /locus_tag="VN627_09240"
     CDS             5381..5875
                     /locus_tag="VN627_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXD03479.1"
                     /translation="MPVHELPGRPWPVELLDELLRVHGRFKTTFADVRLQTGLNETEL
                     MVLNAVAQAKPSATVSQIARSIGTSRQLIQKAANALRDSGLIEMLDNPGHKRASLLHM
                     APRGFAVKGMLDQAAVALADSLAPTIDLGELAAAIEALRKVRRALEARQRQSGKPEPA
                     GKAG"
     gene            6006..>6771
                     /locus_tag="VN627_09245"
     CDS             6006..>6771
                     /locus_tag="VN627_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HXD03480.1"
                     /translation="MRWIEGGEFTMGSLAFYPEEAPLARVRVDGFWIDPHPVTNRQFA
                     EFVAATGHVTFAEIAPDPKDYPGLLPGMDQPGSLVFRKSAVMIGTQDPSRWWEFCLGA
                     DWRHPYGPASSLDGLEDHPVVHVTWNDAVAYARWSGKDLPTEAEWEYAARGGLEGKEF
                     AWGDELAPGGAMMANYWQGLFPFANTLEDGWERTSPVGSFPANGYGLFDMIGNTWEWT
                     RDWWQDNRPKGGDCCVVKNPRGGTLKGSLDRAEPQVR"
BASE COUNT         1126 a         2306 c         2283 g         1056 t
ORIGIN      
        1 cctcaaccac aaggtcattt tcatcgtgac aaacgccatc gatcccgcct ggacctccgg
       61 ctacgcggag cgcttcgact accgggtgga cgtcctgcgc cgccgcaacc tggtcgaggc
      121 ggccacgccc gatggccgcc aggtcgcgcg cagcgaacgg acgctgctga ttgacgagca
      181 gagccacggg ctggtgttct acttcccgcg cagcgatgtc gacatgagcg ccctcgtcag
      241 gatcgatcgg gtcagccact gcccgttcaa gggcgtggcc tcctactttg cgctggccgg
      301 aaacgcggcg ggcgaaccgg tcgcctggag ctacgagacc ccgtaccccg aggtcagcca
      361 gattgccgag catatcgcct tctaccagga ccgcatggtc gtgcgccttg gcgtcgcgat
      421 attccccatt cccaagaagg cgtgaggcag aaccatggcc aacccgacca gcaacgatac
      481 ctacgagcaa cattggggcg acctagccga tctggcccgc atcatcgatc tccagcccac
      541 ggctccgggc gtgttttccg cgccgatcta cgagggcgcg ccgcgcaacg tggtggaggg
      601 cagccagatg ctggcccagt ccatcgtcgc ggccgcgaag cagatgccgg aaaagcgggt
      661 gatatccgcc tatgcccagt ttgcccgcgt cgccgggttc gaccggccgc tggacttcca
      721 tgtcgacgtc ctgcagtccg ggcggacctt cgcctcgctg tcggtcaagg ccgaacagga
      781 cggcaagctg cgctgccccg ccatggtcct gatggacgcc ggctcgcccg acttcatcgc
      841 ccaccaggtg ccgatgccgc aggtgcccgg ccccgacgag tgcccggact acgactatgg
      901 catgaccggg cgcgacgtgc gcttcgtcaa cggcgactac acgccccatg agcaccggat
      961 gggcgatccc gtcctgcatt gctggatgcg gtgccgcgac aagccgaaaa cccagctgat
     1021 gcaccaggcc ctgctgaccc agccggtcgg ccacttcacc atcggcgcgg cgatgctgcc
     1081 gcatcccggg gtcaaggagg gtgatgccca tgtcacgctg tccacgggga tcatgtcgat
     1141 tgccatcgcc tatcatgccg atgcggacgt gagcggctgg gtgctctatt ccaatccggc
     1201 gatctggagc gggcgcggcc tggtccaggg ccatggcacc gtgttcgcgc aggacggcac
     1261 cctgcttgct tcttacacgg taaccggcat ggtcagggaa ttcgcgcccg atgccgcgat
     1321 cgccggaatt gccgcgaacc agttgatgtg acgaagccgg gggcaccgca gaaccgggcc
     1381 ccggaatcgt tgcggtaaga ggatggaacc ggctgccggc gcagcagcct gaatgggaga
     1441 cgatgaatgg atttcggact ggccggcaag gtagccgtcg tcgcaggagg cagccgcggg
     1501 tgcggcctcg ccatcgcgcg tgaactcgcc cgggaaggcg catcggtcgt gctgaccggg
     1561 cgcaaccgcg acatcgtcga ggcggcggcg gaggcgatcc gggcagacgg cggcgcggcc
     1621 tacgggctga ccaccgagat ggtttcggcc gaggatgcgg cccgcatcgc ccaggccgcg
     1681 cgcgacaagt tcggcgatcc cggcatcctc gtcgtcaacc cgccgacccc cggcaatgcc
     1741 gccggcttct tcgaggtgac cgacgaggat tacctgcgcg cgcacgagta tttcaacatg
     1801 ccgctggtgc gcctctcgcg cgaaatgatc ccggcgatgc agcgaggcca gtgggggcgg
     1861 atcgtcaaca tggcctcggt cggcataaag cagcctcacc tcaagttcct gctctacgcg
     1921 accaataccc gcgttgccgc ggcggccatc gccaagacga tgtcgttcga gttcgccaag
     1981 gacaacatca ccgtcaacac cattgccaca gggcccttcc cgtcggcgct ggccgatgaa
     2041 tacaacaagg atgccggcta cgccgccgac cggatcgcca agtccacgcc gatgaagcgg
     2101 cttggccgaa ccgaggagat ggccgcgatg gtctcgttcc tctgttcgga ccgggccagc
     2161 ttcgtctccg gggaaacgat ccgggtcgat ggtggctcga ctttcaatct gttctgatga
     2221 acccttggcc gttcgcgcgg cgcatggcgg gaaggtagcg acatggaact gaatcttgcc
     2281 ggcaagcggg ccctggtcac cggcagcacg gccgggatcg gcgaggccat tgccaagacc
     2341 ctggcgcgcg aaggcgccaa ggtggtgatc aatggccgga gcgagcggcg cggcaaggcc
     2401 gttgccgagg cgatcgaggc agcgggcggc gaagccctgc tggcgctggg caacgtggcc
     2461 agcgacgagg acgccgacag ggtcggccgc gaggtgcgcg acgcctgggg cggggtcgac
     2521 atcctcgtga acaacgccgc cggctttgcc agcggcagca gcgtgggcag cttcttcacg
     2581 gtcacggccg aggagtgggt caagacctac gacctcaatg tcggcgcggc cgtgcgcatg
     2641 atgaacctgc tggtgccgga catggcggag cgcgggtggg gacgcgtcat caatatcggc
     2701 agcagcggag gctggatgcc gaccggggaa ctacctgact acagcgtcgc caagatcgcc
     2761 atgatgggac ttaccgccaa cgccgcccgc gggctttccg ggaccggggt gacagtcaat
     2821 tccgtggttc cgggcgtgat ctacacccgg tccgtggcgg catggttccg cgccattggc
     2881 gagcagcagg gctggggcga tgatcgggcc aagtcggagg cttacgtcct ggcgcggcat
     2941 ggccagacgg taaaccgggt cggcgaggtc gcggatatcg ccaatatggt cgcctacctt
     3001 gccagcccgc tctccggtta tatcaatgcc gtgaacctcc gaattgacgg aggcgcgttc
     3061 tcggccgtcg gctgacgggg cgggaaccac atgtgcgggc ccgccttgtt ggggacctga
     3121 agaatgacag agagtgtcga atgtccccca tcggccaccg gctcgcgccg ccggaacaga
     3181 aattgcaaag ccgggtcccg gcgatggtga cagcccccgc tgacccggca ttcctcgcag
     3241 cggttacaac cggcctgcaa cgcccagtga aggcaatccc gccgcgcttc ttctatgacc
     3301 tcgagggctc gcacctgttc gacgagatca cgcgcctgcc cgaatactat ccgacccgga
     3361 ccgaaacagc cctgctgcgc cgccacgccg aagacctggc agccgctgtc ggcacgggcc
     3421 ggccggtgat cgaattcggc gccggctcgg caaccaagac cccgctgctg ctcgatgcgc
     3481 ttgcgacgca gacctacgtt ccgatcgaca tctcgggcga ttatctcgag caggcggtgg
     3541 cgcagctcgg gcaggcgcgg cccgggctgc ggattgttcc ggtggtgggc gactttacca
     3601 cccctctcgc actgccgccg ctttccggcc cggtgacggg cttcttcccc ggctccacca
     3661 tcggcaattt cgatcccgcc gaggcgatcg gcctgctgcg ctcgttccgg cagctgctgg
     3721 gcgaaggcgg ccggctggtt atcgggatcg atacccgcaa ggaccggcgc gtgctggaag
     3781 ccgcctacga cgatgccgcc ggggtcaccg cggcgttcaa ccgcaacctg ctgacccgga
     3841 tcaaccgcga gcttgccggc acgatcccgg tcgagcactt cgcgcaccgg gcgatctgga
     3901 acgaagcgga ggggcgggtg gagatgcacc tggaagccct gcgcgacctg cgcttcaccg
     3961 tcgccggcga ggccttcgcc atggccggcg gcgagacgat ccacaccgag agctgctaca
     4021 agtacacccc cgccgaggca gaccggctgg cccgcgccgg cgggtgggag ccgctggtct
     4081 gctggaccga cccggaagac ctgttcggcc tccacgtctg gggtgcggta cccgatgcga
     4141 gactaccgtg agcgccggcc gggcggagcg ctataacgaa gtccgcgccg cgaccgaggc
     4201 gctgacctcg ggtctttcgg ccgaggactg ccaggtccag tcgatgcccg acgtcagccc
     4261 ggccaaatgg cacctggctc acaccacctg gttcttcgag acgttcctgc tcctgcccat
     4321 gctggaggga taccggccgc tcgacccggc tttcgcggtc ctgttcaatt cctactatgt
     4381 cggcgtgggc gagcgccacc cgcgccccag ccgcggcctg ctgacccggc cgtcgctgga
     4441 cgaggtccac gcctaccgcc gccatgtcga tgccgcgatg cggcggctga tggagacggt
     4501 tgccgagccg gcttgggcgt ggctggttga actcgggtgc cagcacgagc aacagcacca
     4561 ggagctgatc cttatggata tccagcacgt gctgtcctgc aatccgctgg agccagccta
     4621 tcgccgcccg gatcccgccg gcgaaccgtc cgggccgctg gcctggtccg atcacccggg
     4681 cggcctgtgc gaaatcggcc atggcggcga cgggttcgcc ttcgacaacg agggcccgcg
     4741 ccaccgcgtc tgggtcgagc ctttcgccgt cgccaaccgc cttgtcaccg ccggcgaata
     4801 cctcggcttc atcgaggatg gcggctaccg tcgccccgag ctgtggctgt cggacgggtg
     4861 gaccaccgtg gaagcgcagg gctggcaggc cccgctctac tgggacaaat ccggcgggca
     4921 atggacccgg ttcaccctgg ccggccggcg tcaagtcacc ctatccgaac cggtctgcca
     4981 cgtcagctac tacgaggccg aagcctatgc ccgctgggcg ggggcgcgcc tcccgagcga
     5041 ggccgagtgg gaactggccg ctgccgatcc ggccatggtc cagttgtacg acacagcctg
     5101 gcaatggacc gcatcgccct atgtgggcta tcccggctat gtcccgctca agggcgccgt
     5161 tggcgaatac aacggcaagt tcatgatcaa ccagcaggtc ctgcgcggcg gttcgctggc
     5221 gacgccggcg ggacacagcc gcgccaccta ccgcaacttc ttccctccgg ccgcgcgctg
     5281 gctgttcggc ggcatccgcc tggcgaaggg ctggaatggc gccgcacccc ggcaggcagc
     5341 gccggattga caccttcggt ccaaccccta agggcgcgcg atgcctgttc atgaactgcc
     5401 tggacggccc tggccggtcg agctgctcga cgaactgctg cgggtccacg ggcggttcaa
     5461 gaccaccttt gccgatgtgc ggctgcagac cgggctcaac gagaccgagc tgatggtgct
     5521 caacgccgtc gcccaggcga agccctcggc caccgtttcc cagatcgcgc gatcgatcgg
     5581 cacctcgcgg cagctcatcc agaaggccgc caacgccctg cgcgattccg gcctgatcga
     5641 gatgctcgac aatcccggtc acaagcgcgc ctcgctcctg cacatggcgc ccaggggctt
     5701 cgcggtgaag ggcatgctcg atcaggcggc ggttgcgctc gccgattcac tggcgccgac
     5761 catcgacctt ggcgaacttg cggctgccat cgaagcgctg cgcaaggtgc gccgcgcgct
     5821 ggaggcgcgg cagcggcaga gcggcaagcc cgaacctgcg ggcaaggccg gctagtcgcc
     5881 ggccggaatg ggctcagcgc catcgggcag cgcggcgacg gcgtctgctt cagccggaca
     5941 gggcgccttg tcagccttgc cgcggcggct ctaagagatt gcggatgatg caggattccc
     6001 cgtcaatgcg ctggatcgaa ggcggtgagt tcacgatggg gtcgctcgcc ttctatcccg
     6061 aggaagcccc gctggcgcgc gtgcgggtcg acgggttctg gatcgatcca cacccggtca
     6121 ccaaccgcca gttcgccgaa ttcgtcgcgg ccacgggcca cgtcaccttc gcggaaatcg
     6181 cgcccgatcc gaaggattac ccgggcctgc tgcccggcat ggaccagccc ggctcgctgg
     6241 tcttccgcaa gagcgcggtg atgatcggaa cgcaggaccc gtcgcgctgg tgggagttct
     6301 gcctcggggc agactggcgc cacccctatg ggccggcctc cagccttgac gggctggagg
     6361 atcacccggt ggtccacgtc acctggaacg atgccgtggc ctacgccaga tggtccggca
     6421 aggacctgcc gaccgaggcc gagtgggaat atgccgcgcg cggcgggctg gagggcaagg
     6481 aattcgcctg gggcgacgaa ctggccccag gcggggcgat gatggccaat tactggcagg
     6541 gcctgttccc ctttgccaac acgctggaag acgggtggga acgcacctcg ccggtcggca
     6601 gcttccccgc caacggctac ggcctgttcg acatgatcgg caacacctgg gaatggacgc
     6661 gggactggtg gcaggacaac cgccccaagg gcggcgactg ctgcgtggtg aagaacccgc
     6721 gcggcggcac gctcaagggc agcctcgacc gggccgagcc gcaagtccgg a
//
LOCUS       DATNBV010000385         4694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_528531,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000385 DATNBV010000000
VERSION     DATNBV010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528531"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VN627_09250"
     CDS             <1..760
                     /locus_tag="VN627_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HXD03481.1"
                     /translation="TVLDVKIAMAPTHAASKPVAMIPNCAATRHAHFTLDGSGPKFLE
                     APKLDEWPKVQWAPDWAARRVNLDTLTAEEVKSWKQGDRILLNGKMLTGRDAAHKRIQ
                     DMLAKGEALPVDFKGRVIYYVGPVDPVRDEVVGPAGPTTATRMDSFMDMMLGQGLLAC
                     VGKAERGPAATESIAKHESAYLMAVGGAAYLVARAIKGAKVVGFADLGMEAIYEFDVV
                     DMPVTVAVDSAGQNVHKLAPLVWKKKIAEEHLLA"
     gene            870..1136
                     /locus_tag="VN627_09255"
     CDS             870..1136
                     /locus_tag="VN627_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03482.1"
                     /translation="MALVMVFLMGIANFAMHRAVLASGHPILAQIGWLYRSLGGRFSL
                     IVEFALLVGTMLLVASGGGWWAWGYGLYTGMNAFSAWLILTGKV"
     gene            1174..2004
                     /locus_tag="VN627_09260"
     CDS             1174..2004
                     /locus_tag="VN627_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXD03483.1"
                     /translation="MTGILWLVANRASGSNSDEAVRAVCAALSAAGRAPARVSDVGEG
                     LPDLAALEAGGVGVLAVFAGDGTVNALATRIEGWGGRLLVLPGGTANLLARALHGERE
                     APEIAAGLGQLAVTRRSCIRGEGLTALVEVLAGPGATWSDVREGLREGGVGEVAGAAV
                     TAIKQSTTGPMVALVEPPLGRADGYAGIRLEVAEAGMDVAGYGAETVGDYLKQGLALL
                     RRNFREGPHDALGQHRTVRCRSLDRAPMPLMIDGERREGGADLRFSLADFALDLFVTP
                     "
     gene            2004..2861
                     /locus_tag="VN627_09265"
     CDS             2004..2861
                     /locus_tag="VN627_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXD03484.1"
                     /translation="MTAPLTLFHISDLHFGLEDGRALAWTKAAIARERPAAVAVTGDL
                     TMRARRREFAAACHWIGDLGAPVTVEPGNHDMPYFNLAERFVAPFGRFRAIEGLLEQP
                     LDLGDLHIVPLQTVTRAQWRFPWVDGWVRRPALAKCLALIDALPPGARVLVTAHHPLP
                     ERAPDGHRLTINGSRAMAELARRGVLAVLTGHVHDPFDLVAETPHGPLRMIGAGTLSR
                     RIRSTPPSFNQLTVMGREVQVKVRNLEAVPTSEMQVDEVPAEALPPRQPGKPVAPVAA
                     VPAVDPPVH"
     gene            complement(2866..3507)
                     /locus_tag="VN627_09270"
     CDS             complement(2866..3507)
                     /locus_tag="VN627_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HXD03485.1"
                     /translation="MPYRQLARAAARATGRLGWARGATGALLGLGLAGLLGRIVNGAV
                     TPFMVAPMGAAAVLVFVVPASPLAQPWSVIGGNILSTLIGLAVHALLPVTPWCGALAV
                     ALAIAGMGLARCLHPPGGACALLACLGTGPAGPLLGAVLANVAALVGTGWVWNNATGH
                     AWPHHARPVAPPPSYTMDDLDAVLEDWDEVLDVSREDLDALFRSVEARVNARR"
     gene            complement(3728..4261)
                     /gene="rpsI"
                     /locus_tag="VN627_09275"
     CDS             complement(3728..4261)
                     /gene="rpsI"
                     /locus_tag="VN627_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HXD03486.1"
                     /translation="MSDNTVTDLADLGALAGGAPAVAAPEAGAPEAPVRTGPAAPLRE
                     KEIDAQGRSYATGRRKDAVARVWIKPGSGKITVNGRDQEVYFARPTLRLVINQPFAVA
                     ERSGQYDVVCTVKGGGLSGQAGAVKHGIAQALSKFEPALRSAVKAAGFLTRDPRVVER
                     KKYGRAKARKSFQFSKR"
     gene            complement(4261..>4694)
                     /gene="rplM"
                     /locus_tag="VN627_09280"
     CDS             complement(4261..>4694)
                     /gene="rplM"
                     /locus_tag="VN627_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HXD03487.1"
                     /translation="EKKWHLIDADGLVVGRLAVIIANILRGKHKPSFTPHVDCGDHVV
                     VINAGKVRLTGNKLKQKVYYKHTGYAGGIKEVTAGKVLDGRFPERVLEKAVERMIPRG
                     PLGRAQMRALHLYAGAEHPHGGTQPQPLNVAARNRKNKVGA"
BASE COUNT          694 a         1524 c         1758 g          718 t
ORIGIN      
        1 gaccgtgctc gacgtcaaga tcgcgatggc cccgacccac gccgcctcca agccggtcgc
       61 gatgatcccc aactgcgcgg ccacccggca cgcgcatttc acgctggacg gttcggggcc
      121 gaagttcctc gaggcgccca agctggacga gtggcccaag gtccagtggg cgccggattg
      181 ggcggccagg cgggtcaacc tcgacacgct gaccgccgag gaagtgaaga gctggaagca
      241 gggcgatcgg atcctgctta acggcaagat gctgaccggc cgcgatgccg cgcacaagcg
      301 catccaggac atgctcgcca agggcgaggc gctgccggtc gatttcaagg gccgggtgat
      361 ctactatgtc gggccggtcg atccggtgcg cgacgaggtc gtcggcccgg ccggccccac
      421 taccgccacc cgcatggaca gcttcatgga catgatgctc gggcagggcc tgctcgcctg
      481 cgtcggcaag gccgagcgcg gcccggcggc gaccgaatcg atcgcgaagc atgagagcgc
      541 ctacctcatg gcggtgggcg gcgcggccta cctggtggcg cgggcgatca agggcgcgaa
      601 ggtcgtcggc ttcgccgatc tcggcatgga ggcgatctac gagttcgacg tggtcgacat
      661 gccggtgacg gtggcggtcg attcggccgg gcagaacgtg cacaagctcg cgccgctggt
      721 gtggaagaag aagatcgccg aagagcattt gctggcttga gcagtgatcc tccctgcgag
      781 cgggaggatc ggatcagccg cgacgaagcg ccgctaacgc cgggcaggcc gcgctggcgc
      841 gggcgatcgg agccgctaca ctggcggcaa tggcgctggt gatggttttc ctgatgggca
      901 ttgcgaactt cgcgatgcac cgcgcggtcc tggcttcggg ccatccgatc ctggcccaga
      961 tcggctggct ctatcgctcg ctgggcggga ggttcagctt gatcgtggaa tttgccctgc
     1021 tggtcggcac catgctgctg gtcgccagcg gcggcggctg gtgggcctgg ggctatgggc
     1081 tctacacggg gatgaacgcc ttctccgcct ggctgatcct gaccggcaag gtttgatcgg
     1141 aaccggccgg cccctcgcgg gattgggcga aggatgacag gtatcctgtg gctggtggcc
     1201 aatcgcgcaa gcgggagcaa ttcggacgag gcggtgcggg cggtctgcgc ggccctgtcg
     1261 gcggcaggcc gggcgccggc gcgggtgagc gacgtgggtg aaggcctgcc cgacctcgcc
     1321 gcgctggaag cgggcggcgt gggcgtgctg gcggtctttg ccggcgacgg cacggttaac
     1381 gcgctggcca cgcgaatcga gggctggggc ggccgcctgc tggtcctgcc cggcggcacg
     1441 gccaacctgc tggcgcgcgc gcttcatggc gagcgcgagg cgccggagat cgcggcaggg
     1501 ctgggccaac ttgccgtcac ccggcggtcc tgcattcgcg gagagggcct gaccgcgctg
     1561 gtcgaggtac tggcggggcc cggcgcgacg tggagcgacg tgcgcgaggg cctgcgcgaa
     1621 ggcggcgtgg gcgaagttgc cggggctgcc gtgacggcga taaagcagag cacgaccggg
     1681 ccgatggtgg ccctcgtcga gccgccgctc ggccgggcgg acggctatgc cggcattcgc
     1741 ctggaggtgg cggaggcggg aatggacgtg gcgggctatg gcgccgaaac cgtgggcgat
     1801 tacctgaagc agggcctcgc cctgctgcgg cgcaatttcc gcgaggggcc gcatgacgcg
     1861 cttggtcaac accgcacggt gcgctgccgt tcactcgacc gcgcgccgat gccgctgatg
     1921 atcgatggcg aacggcgcga gggtggggcg gacctgcggt tttcgcttgc ggacttcgcg
     1981 ctcgacctgt ttgtcacccc gtgatgaccg cgccgctcac cctgttccac atcagcgatc
     2041 tccacttcgg gctggaggat ggccgcgcgc tggcctggac gaaggcggcg atcgcgcggg
     2101 agcggccggc ggccgtggcg gtaaccggcg atctcaccat gcgcgcgcgg cggcgggagt
     2161 tcgcggcggc ctgccactgg atcggcgacc tcggcgcgcc tgtcacggtc gagccgggca
     2221 atcacgacat gccctatttc aaccttgcgg agcgcttcgt cgcaccgttt ggccggttcc
     2281 gggcgatcga gggcctgctg gaacagccgc tcgatctggg cgacctgcac atcgttccgt
     2341 tgcagacggt gacccgggcg cagtggcggt ttccctgggt ggacggctgg gtgcgccgcc
     2401 cggccttggc gaagtgcctg gcgctgatcg atgccctgcc gcccggcgcg cgggtgctgg
     2461 ttaccgcgca ccaccccctg cccgagcgcg cgcccgacgg gcaccggctg accatcaatg
     2521 gcagccgggc catggccgag ttggcgcgta ggggcgtgct ggccgtgctg acgggccatg
     2581 tccacgatcc gttcgacctc gttgccgaga caccgcacgg ccccttgcgg atgatcggcg
     2641 cgggcacgct ttcgcggcgc atccgctcga ccccgccgag tttcaaccag ctgaccgtca
     2701 tgggccgcga ggtgcaggtg aaggtgcgca atctcgaagc cgtgccgacc agcgagatgc
     2761 aggttgatga ggtgccggcc gaggccctgc cgccgcgcca gccgggcaag ccggtggcac
     2821 cggtcgcggc agttccggcg gtggacccgc cggtccactg agcacttagc ggcgggcgtt
     2881 cacccgcgct tcgaccgagc ggaagagggc gtcgaggtct tcccggctga cgtcgagcac
     2941 ttcgtcccag tcctccagca ccgcgtcgag gtcgtccatg gtgtaggacg gcggcggagc
     3001 gacggggcgc gcgtggtgcg gccaggcgtg gcctgtcgca ttgttccaga cccagccagt
     3061 gccgacgagg gccgcgacat tggccagtac ggcgccgagc agcgggccgg caggccccgt
     3121 gccgagacag gcgagcaggg cgcaggcgcc gcccggcgga tgaaggcagc gcgccaggcc
     3181 catcccggcg atcgccagcg ccacggcaag ggcgccgcac catggcgtga cgggaagcag
     3241 ggcatggacc gcaaggccga tcaaggttga caggatgttg ccgccgatca ccgaccatgg
     3301 ctgggccagc ggactggcgg gcacgacgaa gaccagcacg gccgcggcac ccatcggcgc
     3361 caccatgaac ggcgtgaccg cgccgttcac gatcctcccg agcagtccgg ccagacccag
     3421 tccgaggagc gcgcccgtgg ctccgcgcgc ccagccgagg cggcccgtgg cgcgggcggc
     3481 ggcccgggcg agttgacggt atggcatggg aagtcggtgt tccgtcagta gaaggaggtg
     3541 gcccagtatc gcagcagctc ccacttgcct accggcttag cgcgaacggc cggatcgcag
     3601 cccagcgagc gcaggctgat ctcggccagc tgtgcaatcg atccctggat gaggcagaca
     3661 aacgaaaacg ccgggggaaa tccccgggcg tttcctgtac ttgccgcgaa ggcgcgatcg
     3721 ggggggatca gcgcttcgag aactggaagg acttgcgagc cttggcccgg ccgtacttct
     3781 tgcgttcgac gacgcgcggg tcgcgggtga ggaagcctgc cgccttgacc gcgctgcgca
     3841 gggccggctc gaacttcgag agtgcctggg cgatgccgtg cttgaccgcg ccggcctggc
     3901 ccgaaaggcc accgcccttg acggtgcaga cgacgtcgta ctggcccgag cgttcagcca
     3961 ccgcgaacgg ctggttgatc acgaggcgca gggtcggacg ggcgaagtag acttcctgat
     4021 cgcggccgtt gacggtgatc ttgcccgagc cgggcttgat ccacacgcgg gccacggcgt
     4081 ccttgcggcg gccggtggcg taggaacggc cctgggcatc gatttccttt tcgcgcaggg
     4141 gcgcggcggg accggtgcgg acgggagctt ccggagcgcc ggcttcgggc gcggcgacgg
     4201 cgggagcgcc gccggcaaga gcgcccagat cggcgagatc ggtgacggtg ttatcggaca
     4261 tcaggcaccc accttgttct tgcggttgcg ggcagcaacg ttgagcggct gcggctgggt
     4321 gccgccatgc ggatgctcgg cgccggcgta gagatgcagg gcgcgcattt gggcgcggcc
     4381 caggggcccg cgcgggatca tgcgctcaac cgccttctcg agcacgcgct cggggaaacg
     4441 gccgtccagc acctttccgg cggtcacttc cttgatgccg ccggcatagc cggtgtgctt
     4501 gtagtagacc ttctgcttga gcttgttgcc cgtcagccgc accttgccgg cgttgatcac
     4561 gacgacatga tcgccgcaat ccacgtgggg ggtgaagctc ggcttgtgct tgccgcgcag
     4621 gatattggcg atgattacgg ccaggcggcc gaccaccagg ccatcggcat cgatcagatg
     4681 ccacttcttt tcca
//
LOCUS       DATNBV010000386         4560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1203598,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000386 DATNBV010000000
VERSION     DATNBV010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203598"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VN627_09285"
     CDS             <1..811
                     /locus_tag="VN627_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXD03488.1"
                     /translation="FTQDMDATVRWRLDMEVELRRAIALDHLTMVYQPIVNVADGSIA
                     SFEALVRWNHRDRGEIGPGIFVPVAEQAGLMPQLGDWVLRRVFADSHDFGNAEISVNL
                     SPLQLVARDFLPSLKMMILDEKVDPARFTFEITEGVLLDRSERVMELITELQDMGFRI
                     ALDDFGTGYSSLAYLRAFPFDRIKIDRSFVQGIESDIDAQSILRAIVALGRTLRMKVV
                     AEGVETLLQQQLVHAAGCQLIQGHLYWRALAPEQVIALLATDRVQDLRIAV"
     gene            complement(856..1671)
                     /locus_tag="VN627_09290"
     CDS             complement(856..1671)
                     /locus_tag="VN627_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03489.1"
                     /translation="MEKFENGCAVITGGSGLLGRAMAKAFAREGMDVVVADINDAAMA
                     EVVAEVEALGRSAIAVKTDVSRAEDMDALADAAWARFGKVNLLCLNAGRAMLKPVLEL
                     TREEWDSILGPQFHGVMNGIHSFMKRMAAQDGERHIVVTSSMSGVGRADMRTLNAAYV
                     TAKFATAGLVEVLAPSLAEVGMGISVLCPGMSVADPESLRGVQWPMESAAFYADNLLS
                     AEQVAQEVVHGVREGRLHIFPHRKGRDEVLARHAMLMKGFDQAERTSPPMDAG"
     gene            complement(1700..2584)
                     /locus_tag="VN627_09295"
     CDS             complement(1700..2584)
                     /locus_tag="VN627_09295"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HXD03490.1"
                     /translation="MTGPRIENAIDMHVHFGPDAIRGAELGAGGHSVTAIEAAREAKE
                     TGHRGLVLKSHSFASPQLAENLAREIEGLEVFGGICTDQPSGGLNVEGVATALALGAK
                     VVWLPTVHSHQEWRNGKAEQMGLIGEGIRVIDDSGRPVEQVRAIFDLCQQYGAVLATG
                     HTTLEEHHAIVKAFAREGKVLVTHAGERLAGPHLTPEQARELAEMGAYVELTAQCCDP
                     LFGHEPKTPEQMVEQLRHIGCDRCVLSTDYGWTTAVPHPAPGMVSFLERLWQHGLSED
                     DLTTMAARNPARLLGLEG"
     gene            complement(2666..3406)
                     /locus_tag="VN627_09300"
     CDS             complement(2666..3406)
                     /locus_tag="VN627_09300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXD03491.1"
                     /translation="MHETVAIRTADGTCPAHVFTPASGSGPWPGVIFYMDALAIRPAL
                     LSMAQRLADAGYLVLLPDLFYRYGPYETIDPGPIFAAGSVREVIGPLMATTGKGPATR
                     DTAAFLDWFNSRADFAGGKLGVTGYCMGGGIALAVAAAYPNRIGAAASVHGGSLATDE
                     PDSPHLGASRIKARVYVAGADQDASYPPEQAERLEAALTAAGVDHKCEIYPGALHGWT
                     MPDFPVYDAVAAERHWDELLSLFAAATG"
     gene            complement(3476..3976)
                     /locus_tag="VN627_09305"
     CDS             complement(3476..3976)
                     /locus_tag="VN627_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HXD03492.1"
                     /translation="MTGRVLGALAALFMTMAMPAPAQAGIQCVAFARAHSLIQLAGNA
                     RDWWARAIGTYARGQAPKAGAVLAFRSTGSMPMGHVAVVSKVLDARHVLLDHANWSRP
                     GMVEKGVMAVDVSAAGDWSAVRVWHSPTGSLGLRASPAFGFIYPVAEAAMATASIAGA
                     ALDRAR"
     gene            4244..>4560
                     /locus_tag="VN627_09310"
     CDS             4244..>4560
                     /locus_tag="VN627_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03493.1"
                     /translation="MRREPHPITGAIYEELEGGKVRVDYAKIGKQGVFTWDGKWLEGD
                     LTYADPHFLNYVGGPTLPEGMDRYWGFTPPYEEEGAELAHVAGGGSRQALTAQEPIIA
                     MYQP"
BASE COUNT          741 a         1490 c         1544 g          785 t
ORIGIN      
        1 ctttacccag gacatggacg cgaccgtgcg ctggcggctc gacatggagg tcgaactgcg
       61 ccgcgcgatc gcgctcgacc acctgaccat ggtctaccag ccgatcgtca acgtggccga
      121 cggatcgatc gccagtttcg aggcgttggt gcgctggaac catcgcgacc gcggcgagat
      181 cggcccgggg attttcgtgc cggttgccga acaggccggc ctcatgccgc agctgggcga
      241 ttgggtgctg cgccgggtct ttgctgacag ccacgatttc ggcaatgccg agatctcggt
      301 caacctctca ccgctgcagc tggtggcgcg cgatttcctg ccgagcctca agatgatgat
      361 ccttgacgag aaggtcgatc ccgcgcggtt caccttcgag atcaccgagg gcgtgctgct
      421 cgatcgctcc gagcgggtaa tggaactcat caccgaattg caggacatgg gctttcgcat
      481 cgcgctcgat gatttcggca ccggctattc gtcgctcgcc tacctgcgcg ccttcccgtt
      541 cgaccggatc aagatcgacc gctccttcgt ccaggggatc gagagcgata tcgacgcgca
      601 gtcgatcctg cgcgccatcg tcgcactggg gcggacgctg cggatgaagg tggtggccga
      661 gggggtggag acgctgctcc agcagcagct ggtccacgcc gccggttgcc agctcatcca
      721 gggccatctc tactggcggg cattggcgcc cgaacaggtg atcgccctgc ttgcgacaga
      781 ccgggtgcag gacctcagga tcgccgtcta gagcggtttc caatcacggt gacccagatt
      841 ggggggaaaa ccgctctagc ctgcgtccat tggcggcgaa gtgcgctccg cctggtcaaa
      901 gcctttcatc agcatggcat ggcgcgcgag cacttcgtcg cggcccttgc ggtgcgggaa
      961 gatgtgcagg cgcccttcgc gcacgccgtg gacgacctcc tgggccacct gttcggccga
     1021 gagcaggttg tccgcataga aggccgcact ttccatcggc cactgcaccc cgcgcaacga
     1081 ttccggatcg gccacgctca tccccgggca cagcaccgaa atgcccatcc cgacctcggc
     1141 cagcgagggg gccagcacct cgaccagacc ggcggtcgcg aacttggcgg tcacgtaggc
     1201 ggcgttgagc gtgcgcatgt cggcccggcc gacgcccgac atcgaggagg taacgacgat
     1261 gtggcgttcg ccatcttggg ccgccatgcg cttcatgaag gaatggatgc cgttcatcac
     1321 gccgtggaac tgcgggccga gaatggaatc ccactcctcg cgcgtgagtt cgagcacggg
     1381 cttgagcatg gctcgtccgg cgttgaggca cagcaggttg accttgccga accgcgccca
     1441 tgcggcgtcg gcgagagcgt ccatgtcctc tgcccgggac acatcggttt ttaccgcgat
     1501 ggcgctgcgg cccaatgcct cgacctcggc aacgacctcg gccatcgccg cgtcgttgat
     1561 atcggcgacc accacgtcca tcccttcccg cgcgaaggcc ttggccatgg cgcggcccag
     1621 caagccactg ccgccggtga tcaccgcgca gccgttttca aacttttcca tttccgtctc
     1681 cggttatcga acccgcggct cagccttcga ggccgagcag gcgggccggg ttacgcgcgg
     1741 ccattgtcgt caggtcgtct tcactcaggc cgtgctgcca cagccgttcg agaaacgaga
     1801 ccattccggg ggcagggtgg gggaccgcag tggtccagcc atagtcggtg gacaggacac
     1861 agcggtcaca accgatgtgg cggagttgtt ccaccatctg ttccggggtc ttgggctcgt
     1921 ggccgaacag cggatcgcag cactgggcgg tcaattcgac ataggcgccc atctccgcca
     1981 gttcccgggc ctgctccggg gtgaggtgcg gaccggcgag ccgctcgcct gcgtgggtga
     2041 ccagcacctt gccttcccgg gcgaaggcct tgacgatggc gtggtgctct tcgagcgtgg
     2101 tgtggccggt ggcaagcacg gcgccgtatt gctggcacag gtcgaagatc gcccgcactt
     2161 gctcgacagg gcggccagaa tcgtcgatca cgcgaatgcc ctcaccgatc aggcccatct
     2221 gttcggcctt gccgttgcgc cattcctggt ggctgtggac ggtgggaagc cacacgacct
     2281 ttgcgcccag ggccagggcc gtggccacgc cttcgacatt gagccctccg cttggctggt
     2341 cggtgcagat gccgccgaaa acttccagac cttcgatttc ccttgcaagg ttttccgcca
     2401 gttggggcga ggcaaagctg tgggatttca gcacgagccc gcggtggccg gtctccttcg
     2461 cttcccgcgc cgcttcgatc gcagtgaccg aatggccacc ggcgcccagt tcggccccgc
     2521 gaatggcgtc tgggccgaag tgaacgtgca tgtcgatggc gttttcgatg cgtggcccgg
     2581 tcatcgcagc atcctctccc tcggcgggcg aatgccgcct tgcggctgga ccggatggtt
     2641 gcgcgcttcg cccgggggcg caagctcagc cggtggcggc ggcaaacagg ctgagcagtt
     2701 catcccagtg ccgctcggcc gcaactgcgt cgtaaaccgg gaaatccggc atggtccagc
     2761 cgtgcagggc gcccggatag atttcgcact tgtgatcaac tccggccgcg gtgagcgccg
     2821 cctccagccg ctcggcctgt tccggcgggt agctggcatc ctgatcggcg ccggccacat
     2881 agaccctggc cttgatccgc gaagcgccca gatgaggact atccggctcg tcggtggcaa
     2941 ggctgccgcc atggacgctg gcggcggcgc cgatccggtt gggataggcc gcggccacgg
     3001 cgagcgcgat gccgccgccc atgcaatagc cggtgacgcc tagcttgccg ccggcgaaat
     3061 cggcgcggct gttgaaccag tcgagaaagg ccgccgtatc acgggttgcc ggtcccttgc
     3121 cggtggtggc catcagcgga ccgatgactt cgcgcaccga gccggcggcg aagatcgggc
     3181 cggggtcgat cgtttcgtag ggcccgtagc ggtagaacag gtcgggcagg agaacgaggt
     3241 agccggcatc agcgaggcgt tgggccatgc taagcagtgc cggccggatc gcgagcgcat
     3301 ccatgtagaa gatgacgccg ggccacggtc cgctgccgct ggccggtgta aacacgtggg
     3361 ccgggcaggt gccatccgcg gtgcggatcg cgacagtctc gtgcatgcgg atctcctgaa
     3421 cttcgttttg ttcaggatag ggcaggggca gtcccgtgca agagggggca ggggcctagc
     3481 gcgcccggtc gagcgcggcg ccggctatcg aggcagtcgc catggcggct tcagcgaccg
     3541 ggtagatgaa gccgaacgcc gggctggcgc gcaggcccag gctgccggtg ggcgaatgcc
     3601 agacgcgcac cgcgctccag tctcccgcag ccgagacatc gaccgccatc acccccttct
     3661 cgaccattcc ggggcgcgac cagttggcgt ggtcgagcag tacgtggcgc gcatcgagca
     3721 ccttgctgac cacggccacg tggcccatcg gcatggaacc ggtgctgcgg aaggcgagca
     3781 cggcgcccgc cttgggcgcc tggccgcggg cgtaggtgcc gatcgcccgc gcccaccagt
     3841 cgcgcgcatt gcccgccagc tgtatcagcg aatgtgcccg cgcgaaggct acgcactgga
     3901 tgccggcctg cgccggtgcc ggcatggcca tagtcatgaa cagcgcggcc agcgcgccaa
     3961 gaacccgtcc cgtcatgggc gaggcttgtc gccggttgcg ggggattcgc gaagattgct
     4021 ccggctcggc ctgtcccttg ccgccaactg ctcgcctcat ccccggggtg tgcttgtgga
     4081 caagtccgag accccgccgg tgcggcggag tttttgcgtc gcaggtgttg cggctattgg
     4141 cttaacgtca cacgagtggt aacctaatca cgaccgacgc cgtaagcaac ggagtcgcgg
     4201 gacgagaggc cgtgcccaaa gcgcggcgcg agaggagaag atgatgcggc gcgaacccca
     4261 cccgatcacc ggggccattt acgaggaact ggaaggcggc aaggtccgcg tggactatgc
     4321 caagatcggc aagcagggcg tgttcacctg ggacggcaag tggctggaag gcgatctgac
     4381 ctacgccgac ccgcatttcc tcaactatgt gggcgggccc accctgcccg agggcatgga
     4441 ccgttattgg ggcttcactc ctccctatga agaagaaggc gccgagctgg cccatgtggc
     4501 ggggggcggc agccgtcagg cgctgaccgc gcaggagccg atcatcgcga tgtatcagcc
//
LOCUS       DATNBV010000387         3836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1204261,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000387 DATNBV010000000
VERSION     DATNBV010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204261"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VN627_09315"
     CDS             <1..257
                     /locus_tag="VN627_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03494.1"
                     /translation="CFMGLMATVGHYFLILAFQRAPAATLSPYLYGQIGFSMLAGWLV
                     FSHVPDDWSMLGIAMIALCGAGGAWLTVRESRLALEPIEA"
     gene            268..903
                     /locus_tag="VN627_09320"
     CDS             268..903
                     /locus_tag="VN627_09320"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXD03495.1"
                     /translation="MAQYFEVHPENPQVRLLKQAVALLESGGIVAVPTDSSYALACHL
                     DDKSAVDRLRQVRGMDHRHHLTLMCRDLSELSNYARVDNRQYRLLKSATPGAYTFILD
                     ATREVPRRVSHPQRKTVGLRVPDHRVLQQLLAMHGAPLLATTLIPPHETEPLNDAQEI
                     RERFERQIAGVIDAGACPSQPTSVIDLTPMGVGGEAVVVRRGRGDIAALGL"
     gene            1107..1796
                     /locus_tag="VN627_09325"
     CDS             1107..1796
                     /locus_tag="VN627_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXD03496.1"
                     /translation="MSETIRLVDIPNLVQTVLIYALPVLFAITIHEAAHGYVARYLGD
                     NTAYMLGRVTLNPIKHIDPVGTILMPLLLYFATSGAFLFGYAKPVPVNFARLRRPKRD
                     MIWVALAGPASNFVQAMLWAIVYTVLVGTDVNERFFLEMCKAGVLVNLVMWAFNLFPL
                     PPLDGGRILVGLLPRRQAHAVSRLEPWGFFIVMGLVLMGVVGTYWLRPLIMFGYGALD
                     LLLMPLTLLFR"
     gene            1799..3091
                     /locus_tag="VN627_09330"
     CDS             1799..3091
                     /locus_tag="VN627_09330"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXD03497.1"
                     /translation="MPTHPVRYLTGITTTGTPHLGNYVGAIRPAVEASRRAGTENFYF
                     LADYHALIKCDEPARIQRSTLEIAACWLAAGLDPGQVFFYRQSDIPEVTELTWLLTCV
                     TGKGLLNRAHAYKAAVDKNVATGSDPDADVTAGLFMYPVLMAADILMFKAHKIPVGRD
                     QVQHIEMARDMAQSFNHLYGEHFVAPEAAIDESVAMLPGLDGRKMSKSYDNIIPLFAP
                     PEQIRKQIAAIVTDSRLPGEPKQVEGSALFQIYQAFASGEETQALRQAYDSGISWGDA
                     KQLLFERIDREIAPMRERHDALVNNPAELEKILRDGAEKARVLSKPFMAELRAAVGLR
                     SLAAQAGSAGKAKTAKAALPAFKQYREDGRHYFKLVDGSGRLLAQSAGFDSPQQAGRT
                     IAQLKQNGLAGLEPTLELAADVDAADVVAALELLRQLR"
     gene            3155..3778
                     /locus_tag="VN627_09335"
     CDS             3155..3778
                     /locus_tag="VN627_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXD03498.1"
                     /translation="MNAEIKNSKPMSVYVIDDHPLMREAVVMLMRRLRPGANVVELDR
                     IGGVEAAVKQHGPPDLICLDLKLPDTTGTSGIHEMKNRFPETPLAVLSASPAADAEEQ
                     CIEAGADIYIEKSSGAQEIGNALRALLTADGGFEELSPSDNKLSKRQKQLIVMLDRGL
                     SNREIADELGISEHTVKVHLWRLFRRLGVKSRTQTIHYARTHGMLAS"
BASE COUNT          677 a         1287 c         1201 g          671 t
ORIGIN      
        1 tgtgcttcat gggcctgatg gccacggtcg gccattactt cctgatcctc gcgttccagc
       61 gcgcgccggc agccacgctc agtccttatc tgtacgggca gatcggcttc tcgatgctgg
      121 ccggctggct ggtgttctcc catgtgcccg acgactggtc gatgctgggc atcgcgatga
      181 tcgccctctg cggcgcgggc ggtgcctggc tcacggtgcg cgagagccgt ctcgcgctcg
      241 agccgatcga agcctgaaag catcgcgatg gcccagtact tcgaggtcca ccccgaaaac
      301 ccgcaggtac gactgctcaa gcaggccgtc gcgctgctcg aaagcggcgg tatcgtcgcc
      361 gttcccaccg attcgagtta tgcgctggcc tgccacctgg atgacaaatc ggcggttgac
      421 cggctgcgcc aggtccgcgg catggaccac cggcaccatc tcacgctgat gtgccgcgac
      481 ctgagcgagc tgagcaacta cgcgcgggtc gataaccggc agtaccggtt gctcaaatcc
      541 gccacgccgg gcgcctatac cttcatcctg gatgccaccc gcgaagtgcc gcggcgagtg
      601 agccatccgc agcgcaagac cgtcggtttg cgcgttcccg atcaccgcgt gttgcaacag
      661 ctgctggcca tgcacggggc gccgctgctg gccaccacgc tcattccccc gcatgagacc
      721 gagccgctca acgacgcgca ggagattcgc gagcgcttcg aacgccagat cgccggcgtg
      781 atcgatgccg gggcctgccc gtcccagccc accagcgtga tcgatctcac gcccatggga
      841 gtcggcggcg aagccgtggt ggtgcgccgt ggacgcgggg acatcgctgc gctgggtctg
      901 tgaggggctt gtcattgcgg gcgacagcct gagagttgtc atcgcggctc gacccgcaat
      961 ccatgttggc gcgcccgcat ggatcccgga tctagtccgg ggtgacaact accatgtcat
     1021 tgcgggctcg acccgcaatc catgccacgc gagcgatgga tcccggaatc gagtcccgat
     1081 gacagtcagg agggatgaca ccatgcgtgt ctgagacaat ccgcctcgtg gatattccca
     1141 atctcgtcca gacagtcctg atctacgccc tgccggtgct gttcgccatc accatccatg
     1201 aggcggcgca cggctacgtc gcgcgctatc tgggcgacaa cacggcgtac atgctggggc
     1261 gggtcacgct caacccgatc aagcacatcg atccggtggg cacgatcctg atgccgctgc
     1321 tgctttactt cgcgacgtcc ggcgccttcc tcttcggcta cgccaagccg gtgcccgtga
     1381 acttcgcccg cctgcgccgc cccaagcgcg acatgatctg ggtcgcactg gccgggccgg
     1441 cgtccaattt cgtgcaggcg atgctctggg ccatcgtcta tacggtgctg gtgggcaccg
     1501 acgtgaacga gcggtttttc ctggagatgt gcaaagcggg cgtgctggtc aacctggtca
     1561 tgtgggcctt caacctgttc ccgctgcccc cgctggatgg cgggcgcatc ctggtgggcc
     1621 tgttgccgcg gcgccaggcc catgccgtca gccgactgga gccctggggc tttttcatcg
     1681 tgatggggct ggtcctgatg ggtgtcgtcg gcacctactg gctgcgccct ttgatcatgt
     1741 tcgggtatgg cgcgctggac ctgctgctca tgcccctcac actcctgttt cgataaccat
     1801 gccgacccac cccgttcgtt acctcaccgg catcaccacc accggcacgc cgcacctggg
     1861 caactatgtc ggggcgatcc ggccggccgt cgaggccagc cggcgcgccg gtaccgagaa
     1921 cttctatttc ctggccgatt accacgcgct gatcaagtgc gacgaaccgg cccgcatcca
     1981 gcgctccaca ctggagatcg cggcctgctg gctggccgcc ggcctggacc ccgggcaggt
     2041 gtttttctac cgccagtcgg acatcccgga agtgaccgag ctgacctggc tgctgacctg
     2101 cgtcaccggc aagggcctgc tcaatcgcgc ccatgcctac aaggcggccg tcgacaagaa
     2161 cgtggcgacc ggcagcgacc cggacgccga cgtgacggcg ggccttttca tgtacccggt
     2221 tctgatggct gccgacatcc tgatgttcaa ggcccacaag atcccggtgg ggcgcgacca
     2281 ggtacagcac atcgagatgg cgcgcgacat ggcgcaaagc ttcaaccatc tctacggcga
     2341 gcatttcgtg gcgcccgaag cggcgatcga cgagtcggtg gcgatgctgc ccggcctcga
     2401 tggccgcaaa atgagcaaga gctacgacaa catcattccg ctgttcgcgc cgcccgagca
     2461 gatccgcaag cagatcgccg ccatcgtgac cgactcgcgc ttgcccggcg agcccaaaca
     2521 ggtcgaaggc tcggcgctgt tccagatcta ccaggccttc gcctccggcg aggaaaccca
     2581 ggccctgcgc caagcctacg acagcggcat cagctggggc gatgccaagc agctcttgtt
     2641 cgagcgcatc gaccgcgaga tcgcaccgat gcgcgagcgc catgatgcgc tggtgaacaa
     2701 ccccgccgaa ctcgaaaaga tcctgcgcga tggcgctgag aaggcgcggg tcctttccaa
     2761 gccgttcatg gccgaactgc gcgccgctgt cggtctgcgc agcctggcgg cgcaagcagg
     2821 ttccgcggga aaagcaaaaa ccgccaaggc ggcgctgccg gcattcaagc agtaccgcga
     2881 ggacggccgg cactacttca agctggttga tggcagcggg cggctgctgg cccagagcgc
     2941 cgggttcgac tcacctcagc aggcgggccg cacgattgcc cagctcaagc agaacgggct
     3001 cgcgggactc gaacccacgc tggaattagc tgcggatgtc gatgcggccg acgtcgtggc
     3061 cgcgctggaa ctgttgagac agcttcgcta aggccggcgt gctaaaaggc gaagaaaatg
     3121 ggccggaatt gattccgagt tatccttact tcttatgaat gcagaaatca agaactccaa
     3181 accgatgagc gtttacgtca tcgatgacca cccgttgatg cgcgaagccg tcgtgatgct
     3241 gatgcgccgc ctgcgaccgg gcgccaacgt ggtcgagctg gaccgtatcg gcggtgtcga
     3301 agccgccgtc aagcagcacg gtccgcccga tctgatctgc ctggacctga agctgccgga
     3361 taccaccggc acgtcgggga ttcatgagat gaagaaccgc tttccggaga cgccgctggc
     3421 ggtgctgtcc gcctccccgg cggccgatgc cgaggaacag tgcatcgagg ccggcgccga
     3481 catctacatc gaaaagtcct cgggcgccca ggaaatcggc aacgcgctgc gggccctcct
     3541 gactgccgat ggcggtttcg aagaactctc cccgtccgac aacaagctgt ccaagcgcca
     3601 gaaacagctg atcgtgatgc tggaccgcgg cttgtccaac cgcgaaatcg ccgacgagct
     3661 cggtatcagc gagcacaccg tcaaggttca tctctggcgg ctgttccgcc ggctaggggt
     3721 gaaaagccgc actcagacca tccattacgc ccgcactcac ggcatgctgg ccagctgagc
     3781 caccgaagcg gcggcccggt ccgccgcctc ctgcgcgtcg gtgggctgga gcatca
//
LOCUS       DATNBV010000388         4534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2781770,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000388 DATNBV010000000
VERSION     DATNBV010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2781770"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VN627_09340"
     CDS             <1..214
                     /locus_tag="VN627_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03499.1"
                     /translation="ARESLVETEDGAAAAEKYHQSLADPAKVAQLVVDQALARQFWIV
                     PSLDPYGDTVRGRIAELLEAEPTGQL"
     gene            complement(233..1231)
                     /locus_tag="VN627_09345"
     CDS             complement(233..1231)
                     /locus_tag="VN627_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HXD03500.1"
                     /translation="MTQEVFLKARPQGGLRAADLGLREQALPPCGDGDVRLAIRYVSA
                     DPFTRIFLDETVLGAALPGTALGAVLPGAAVGEVVESRSPALRPGDLVEGRFGWREAH
                     VTPAEGLRKLEPALGDGRAALGILGLPGTTAYGGIVTVLGIKPGETVIISSAAGGVGL
                     IAGQIARCLGARAVGIAGGPAKCAMVLEHGFDACIDYKAANFEQALLAACPGGAQAYF
                     DNVGGKVAMAAYAALGRGGRVALCGLLSLYQGEGGDEGDLGRFMRLIMSRGLSIKAFS
                     TVGLDQPAALVDLARWMKEGRIVVPETVVDGLASAPDAFVDLLAGRIAGKLVIKVG"
     gene            complement(1242..1682)
                     /locus_tag="VN627_09350"
     CDS             complement(1242..1682)
                     /locus_tag="VN627_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HXD03501.1"
                     /translation="MSVYTGDILAAIRKVGGDHLDLPFWEATAQQRFLLHRCGICEKH
                     YWPASRCLDHGDAAMAWVPASGLGEVHTYTIVHHAYSPRMAARVPYCVAVVKLAEGPF
                     FHTNITGLPSQEVAVGLEVAVRYEQDEDSGLTLPVFAPAGAAQD"
     gene            complement(1679..2827)
                     /locus_tag="VN627_09355"
     CDS             complement(1679..2827)
                     /locus_tag="VN627_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXD03502.1"
                     /translation="MRSRFAIAALGYTEQGIKLGRSDRDLRGDAVERALQAAGLERDA
                     IDGYIHCWGEREDLRYLGLSPNFNWALSCGGATPSASILAAIGMIEAGQAELVACVYG
                     IAPTSGYIPGNTNLKSIGSYSYGYPSLYGMMGAASAHALHARRHMHLWGTTSEQLGAV
                     AVQQRAYASVRPGTTGFGKPITLEDHQNSPMIVDPFHRLDCCRDTDGGVAVLVCRAER
                     ARQLTDKPIYILGAGTGQNIVNWHKGVPYENHDNIAPAKARAFAQAGITLADIDMAQL
                     YDPFTISVIMQLEAHGFMPEGQGGPFVAAGGMAPDGPIPTNTGGGQLSGFYATGFTAI
                     IEAILQLRGEAGAGQLAKADIALCSGHGMNAGVQNTWNHATLLLGNQP"
     gene            complement(2824..4041)
                     /locus_tag="VN627_09360"
     CDS             complement(2824..4041)
                     /locus_tag="VN627_09360"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXD03503.1"
                     /translation="MTDEIFAGVRIIELAQYVFVPGASALLADQGAEVIKIETVDGGD
                     PYRSLKVHDGRELGNINLAMELNNHGKKSIALDLKTDDGRELLLKLIETADVFLTSLR
                     PQALRKLRLDVDDVRARNPRVIYARGNGLGFKGDEAEKAGFDSSAFWARGGFAHLLTR
                     PDAMQPTRSRPALGDHAGSVALSMGIASALYHRERTGEAKVVETSLLANALWILSGDI
                     TYSQSDTFDEHAYLKQEGRFPLMSAYKTADNRWIQLMLLAPDAFWPGLCELLDIGWCL
                     AEPRFADGQRRMENGPELSEIIRDRLASRTWEEWRPSFEAWNAPWELIRSVREVAADP
                     QAAANGMVYKLAVEDATEVQLVSGPVAFDGQAAPANPRHAPLYGEHTDEVLGSAGIDA
                     ATIAEMRERGVVR"
     gene            complement(4242..>4534)
                     /locus_tag="VN627_09365"
     CDS             complement(4242..>4534)
                     /locus_tag="VN627_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03504.1"
                     /translation="PHRSRVPSDCLTCYRRTRLLSHLAMMDQPPTACAVPIPPSHREP
                     DCSRRIALTTCIYSVIRHYGRYMEKSIGVIAKLADRRNSLIQPAQPQLRSGI"
BASE COUNT          754 a         1582 c         1482 g          716 t
ORIGIN      
        1 cgcacgcgag agcctggtcg agaccgagga cggcgctgcc gcggccgaga aatatcacca
       61 gagcctggcc gatcccgcca aggtggcgca gctggtggtg gaccaggccc tggcccgcca
      121 gttctggatc gtgcccagcc tcgatcctta cggtgatacg gtccggggca ggatcgcgga
      181 actgctggag gccgagccga ccggtcagct gtaaggcgcc cgggcgcgca tctcagccga
      241 ccttgatcac cagcttgccg gcgatccggc cggccaggag atcgacaaac gcgtccggcg
      301 cggaggcgag gccgtctacc accgtttccg gaacgacgat ccgcccttcc ttcatccagc
      361 gcgccagatc gacaagcgcg gcgggctggt ccaggccaac tgtgctgaac gccttgattg
      421 acaggccgcg cgacatgatc aggcgcatga agcggcccag gtcgccctcg tcgccccctt
      481 cgccctgata tagcgagagc aggccgcaca gggcaacgcg tcccccccgg ccgagcgcgg
      541 cataggcggc catcgccacc ttgccgccga cattgtcgaa ataggcctgc gctcccccgg
      601 ggcaggccgc cagcagcgcc tgctcgaagt tggcggcctt gtagtcgata caggcgtcga
      661 acccgtgctc gagcaccatc gcgcacttgg ccggcccgcc cgcgatgccg accgcccgcg
      721 ctccgaggca ccgggcgatc tgccccgcga tcaggcccac cccgccggcg gcggaggata
      781 tgatgacggt ctcacccggc ttgatcccga gaacggtgac gatcccgcca taggccgtcg
      841 tcccgggcaa accgaggata cccagcgcgg cccgcccgtc gccaagcgcc ggctccagtt
      901 tccgcaagcc ttccgccggc gtgacatggg cctcgcgcca gccgaaccgc ccttcgacca
      961 gatcacccgg ccgcagcgcc ggtgaccggg attcgacaac ctcgccgacc gcggctccgg
     1021 gcaggacggc ccccaatgcc gtgccgggca aggctgcgcc aaggacggtc tcatccagga
     1081 agatgcgcgt gaacggatcg gccgagacat agcggatcgc cagccgcacg tccccatcgc
     1141 cgcagggggg cagcgcctgc tcgcgcaagc ccaggtccgc cgcgcgcagt cccccttgcg
     1201 ggcgggcctt gaggaaaacc tcctgcgtca tggcaactcc cctagtcctg ggcggcgccg
     1261 gcgggggcga acacgggcag tgtcaggccg gagtcctcgt cctgctcata gcgcacggcc
     1321 acttcgaggc cgaccgccac ctcctggctg ggcaagccgg tgatgttggt gtggaagaac
     1381 ggcccctcgg ccagcttcac cacggccacg cagtagggca cgcgcgcggc cattcgcggc
     1441 gaataggcat gatggacgat ggtataggtg tgaacctcgc caaggcccga ggccggcacc
     1501 caggccatcg cggcatcccc gtgatcgagg caccggctgg ccggccagta gtgcttctcg
     1561 cagatcccgc accggtgcag caggaagcgc tgctgggccg tggcctccca gaacggcagg
     1621 tcgagatgat cgccgccgac cttgcggatc gcggccagga tatcaccggt atacacgctc
     1681 atggctggtt ccccaacagc agggtcgcat ggttccatgt gttctggacc ccggcgttca
     1741 tgccgtggcc gctgcacaag gcgatatccg ccttcgccag ctgccccgcg ccggcctcgc
     1801 cgcgcaattg caggatcgcc tcgatgatgg cggtgaaccc ggttgcgtag aagccggaca
     1861 attgcccgcc gccggtattg gtcgggatcg gcccgtccgg cgccattccc ccggcggcca
     1921 cgaacggccc gccctgtccc tcgggcatga agccatgggc ctccagctgc atgatgaccg
     1981 aaatcgtgaa cggatcgtac aattgcgcca tgtcgatatc ggcaagggta atgcccgcct
     2041 gcgcgaaggc gcgggccttg gccggggcga tgttgtcgtg gttctcgtag ggcacgccct
     2101 tgtgccagtt gacgatgttc tgcccggttc ccgcgcccag gatatagatc ggcttgtcgg
     2161 tcagctggcg cgcgcgctcc gcccggcaga ccagcaccgc aaccccgcca tcggtgtcgc
     2221 ggcagcagtc cagccggtgg aacgggtcga cgatcatcgg cgaattctgg tgatcttcca
     2281 gggtgatcgg cttgccgaag ccggtcgtcc cgggccgcac cgaagcatag gcccgctgct
     2341 ggaccgcaac cgcgccgagc tgctccgagg tcgtccccca cagatgcatg tgccggcgcg
     2401 cgtggagggc atgggcgctg gccgccccca tcatgccgta gagcgaggga tagccatagc
     2461 tgtagctgcc gatcgacttg agattggtgt tccccgggat gtagcccgag gtcggcgcga
     2521 tgccgtagac acaggccacc agttcggcct gccccgcctc gatcatcccg atcgccgcca
     2581 ggatcgaggc ggaaggggtg gccccgccgc acgacagggc ccagttgaaa ttgggcgaaa
     2641 ggccaaggta gcgcaggtcc tcgcgttcgc cccagcaatg aatgtagccg tcgatcgcat
     2701 cgcgttcgag accggcggcc tgcagggccc gctccaccgc atcgccgcgc aggtcgcggt
     2761 cggagcggcc aagcttgatc ccctgctcgg tatagccgag cgcggcgatc gcgaaccggc
     2821 ttctcaacgc acgacccccc gctcccgcat ctcggcgatc gtggcggcgt cgatccccgc
     2881 cgagccgagc acctcgtcgg tgtgttcgcc atacagcggc gcatggcgcg ggttggcggg
     2941 ggcagcctgc ccgtcgaagg cgacgggccc ggaaaccagc tggacctcgg tggcgtcctc
     3001 caccgccagc ttgtagacca tgccattggc cgcggcctgc gggtccgccg ccacttcacg
     3061 gaccgaccgg atcaattccc acggcgcgtt ccacgcctcg aagctggggc gccactcctc
     3121 ccaggtccgc gaggccagcc ggtcccggat gatctccgaa agctcgggcc cgttctccat
     3181 gcgcctctgg ccatcggcga agcgcggctc ggccaggcac cagccgatgt ccagcagctc
     3241 gcacaggccg ggccagaacg cgtccggcgc cagcagcatc agctggatcc agcggttgtc
     3301 ggccgtcttg taggccgaca tcaacgggaa gcggccttcc tgcttcaggt aggcatgttc
     3361 atcgaaggtg tccgactgcg aataggtgat gtcgccagac aggatccaca gggcattggc
     3421 gaggagcgag gtctccacca ccttggcctc gccggtgcgc tcgcggtggt acagcgccga
     3481 ggcgattccc atcgacaagg ccaccgaacc ggcgtgatcg cccagcgccg ggcgcgagcg
     3541 ggtgggctgc atcgcgtcgg ggcgggtcag gagatgggcg aagccgcccc gcgcccagaa
     3601 ggccgacgaa tcgaacccgg ccttctcggc ttcgtctccc ttgaagccga ggccattgcc
     3661 gcgcgcgtag atcacccggg ggttgcgggc ccgcacgtcg tcgacatcga gccgcagctt
     3721 gcgcaaggcc tgtggccgca ggctggtcag gaacacgtcg gcggtttcga tcagcttgag
     3781 cagcagctcg cgtccgtcgt ccgtcttcag gtccagcgcg atgctcttct tgccgtggtt
     3841 gttcaattcc atggccaggt tgatgttgcc cagctcacgg ccgtcgtgga ccttgagcga
     3901 acggtaggga tcgcccccgt ccactgtttc gatcttgatg acttcagccc cctgatccgc
     3961 cagcagggcc gatgcgccgg gcacgaacac atattgcgcc agttcgatga tccggactcc
     4021 ggcgaaaatc tcatcagtca catcattctc ccgcaccagg cgatgtccag acaatcccgg
     4081 cccgcttctg cgcgccgcca cgggttcgca ttgcgtgcag agttcccgca gcactgttcc
     4141 cgaaccggag tgaattcgct tcccggctcg gctccgcctc ctggaacgga ggtgacggca
     4201 tctgccgttc gcaacccgtc aacccgaacc gccaacaccg gtcagatccc gctgcgcagc
     4261 tgcggttggg caggctggat caggctgttt cggcggtccg caagcttggc aatcaccccg
     4321 attgattttt ccatatatcg accataatgt ctgataacag aataaatgca agtggtgagc
     4381 gcaatgcggc gggagcaatc aggctcgcga tggcttggcg gaatcggcac ggcacacgcg
     4441 gtcggcggct ggtccatcat cgccaggtgg gatagcaatc gggtgcgccg ataacaggtc
     4501 aggcaatctg acggcacccg tgatcggtgc ggga
//
LOCUS       DATNBV010000389         4373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_530230,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000389 DATNBV010000000
VERSION     DATNBV010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530230"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1781)
                     /locus_tag="VN627_09370"
     CDS             complement(<1..1781)
                     /locus_tag="VN627_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXD03505.1"
                     /translation="MRAFLKQPWVIIAVLAWMVGWVALGHILNQAYWNTRFVFPAVEK
                     VDPSFVLVRVDDGRRIDGHAWTAGDDARVLRKVADAGPRAVFLDRRSDSPLDPSLTQQ
                     IAAMGDRVTVVVRGVYSPFGQIALHIPPEFERSKVRVAVSAWQTNLWMFAEKSAGAFS
                     YSSRTYPALSAALAGVAVPANRSFVPDFHVGPLSVRTISAPDLLNGKTGTEWLRGRTV
                     VVSHVRNAPDGVLGYYGAGWRDPVYVDIAAASGLRHGLITPLNGFPLLIVFAVAVFGA
                     MRLRQSLARRGALLGVVVTAVMIPAILQSYGVFMSSCETLLAIVVFFGTRMWLKWRRK
                     VRETSQSGLPNFVALASEKALAEADVVVGVIARYEEFLASIPPELHGECARQIARRFA
                     VGCGARTIHHGEAGFFAWAEPAREPDQQLDHLEGMRALFAAPLLVGGHMFDTTVHFGL
                     DRNHKLDAQSRINTALASASEALRHGRPVELFQADRLANAAWELSLLARIDEGMRNGD
                     IWLAYQPQWDYREARISGAEALIRWNDPVRGPIRPDEFILQAERAGRIDALTYWVLEQ
                     AIGAAEEFNAVGPRFQTSINLSAQLVD"
     gene            complement(1895..3787)
                     /locus_tag="VN627_09375"
     CDS             complement(1895..3787)
                     /locus_tag="VN627_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system amidotransferase"
                     /protein_id="HXD03506.1"
                     /translation="MCGIAGLFHLTTPKPVDPSRVERMCDAMVHRGPDGAGVWTAPGV
                     GLGHRRLSIIDLAGSPQPMHAADGRAVIVFNGEIYNYRELRTELAARGAVFRTDGDTE
                     VILAAWQQWGPACLARLNGMFAFALYDMGQRTLFLARDRLGVKPLHYAALSDGSLAFA
                     SELKGLLTHPLLRREIDPLAVEDYLAWGYVPDHRAILKGVHKLPAGHYLLLRHGSPLP
                     APVRWWEPSFADRRRESAADLEAELLHHLREAVRSRMAADVPLGAFLSGGVDSSSVVA
                     LMAEMSGSPVKTCSIGFAEAGLDESAYARQVANRFATDHHARAVSPDDFGAIGQLAAM
                     FDEPFADASALPTWRVCQLARETVTVALSGDGADEALAGYRRQRFHRHEDRLRQLLPQ
                     GLRAPLFGGLGAIWPKADWAPRPLRAKTTLLALSEPADAAYARALAVTTPDQRDRLYT
                     PEFQRLRRDYRAEQPLLEAMTRAPARSALDRAQYADLTFWLPGDILTKVDRTSMAVSL
                     EAREPLLDHRLVEFAARLPEGLRIRGTQGKWLMKQAMRRYLPEDILMRPKQGFVTPIA
                     QWLRGPLATAARAIAGSTTLARTGWLDPAAVAGMAEAHIAGRSDHSRLLWQLLMLDRS
                     LTHLGL"
     gene            complement(3801..>4373)
                     /locus_tag="VN627_09380"
     CDS             complement(3801..>4373)
                     /locus_tag="VN627_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HXD03507.1"
                     /translation="QAWSRAADRLEAPVPRQVFLPEVPGWHRVGYTPALWWEPRASGA
                     NHRLLGRYADGEGRTVDVFFALYSSQSEGREAGGFGQGALVPESGWDWRAPGPALADG
                     KADRLLGKSTVPRLAETYYRTDSLLTGSNARLKLANIRDRLILRPAPTMLLILSTDQQ
                     PEAAQAATLEAFRRSAGPLGPWMDRLAGLR"
BASE COUNT          810 a         1414 c         1489 g          660 t
ORIGIN      
        1 ttgtccacca gctgggcgga aaggttgatg ctggtctgga acctgggccc gacggcgttg
       61 aattcctccg ctgcgccgat cgcctgttca agcacccaat aggtcagcgc atcgatgcgg
      121 cctgcgcgtt cggcctgcag aatgaactca tcgggacgga tcggcccgcg cacgggatcg
      181 ttccagcgga tcagggcctc ggcgcccgat atacgggcct cgcgatagtc ccactgcggt
      241 tggtaggcca gccagatatc gccattgcgc ataccctcat cgatgcgggc cagcagcgac
      301 agctcccagg cggcgttcgc caggcgatcg gcctggaaca gttcgaccgg ccgcccatgc
      361 cgcagcgcct ccgaggcact ggccagcgca gtattgatcc gcgactgggc atccagcttg
      421 tggttgcgat caagcccgaa atggacggtc gtatcgaaca tgtgaccacc caccagcagg
      481 ggcgcggcaa acagggcccg catgccttcc agatgatcca gctgctgatc cggctcccgc
      541 gccggctctg cccaggcgaa gaacccggct tcgccgtggt gaatcgtccg cgcgccgcag
      601 cccaccgcaa agcggcgggc gatctgccgg gcgcattcgc cgtgaagttc cggagggatg
      661 gaagcgagaa actcttcata gcgagcgatg acgcccacaa ccacgtcggc ctcagctagg
      721 gccttttcac tggctagggc aacgaagtta gggagacccg actggctggt ttcgcggacc
      781 ttgcgccgcc atttgagcca catccgcgta ccgaagaaaa caacaatggc gagcaaggtt
      841 tcgcagctcg acatgaagac gccataggac tgtaagatgg cgggaatcat cacggctgtg
      901 acaaccacgc ccaacagtgc gccgcggcgg gccaacgatt gccgcaatcg catggctcca
      961 aatactgcga ccgcaaatac gatcagcagc ggaaacccgt tcaacggagt gatcagccca
     1021 tggcgcagtc cgctggcagc ggcgatatcg acatacacgg gatcccgcca tcctgccccg
     1081 tagtaaccca gcaccccgtc tggcgcattg cgaacatggc taacgacaac tgttcgcccc
     1141 cgcagccatt cagttccggt tttgccgttc aggagatcag gtgccgagat cgtacgaact
     1201 gatagtgggc ctacatgaaa gtccggaaca aagcttcggt ttgcgggaac agcgacgccg
     1261 gctagagccg cgctcaacgc agggtatgtc cgcgaactgt aagagaacgc accagcggat
     1321 ttttcagcga acatccacag gttggtctgc caggcggaga ccgcaacccg aactttcgaa
     1381 cgctcaaact ccggtggaat gtggagggca atctggccaa aaggagaata tactccccgc
     1441 accaccaccg tcacccggtc ccccatcgcc gcaatctgtt gcgtcaaaga tggatctaag
     1501 ggcgaatcgc tgcggcggtc cagaaacacc gcccgtggcc cggcatcggc aactttgcgc
     1561 aagacgcggg cgtcatcccc cgcagtccat gcatgcccat cgatgcgccg gccatcgtca
     1621 actctgacta gcacgaagga aggatcgacc ttttccactg ccggaaaaac gaaccgagta
     1681 ttccagtaag cctgattgag gatgtgaccc agcgcaaccc agccgaccat ccaggccagc
     1741 acggcgataa tcacccaggg ttgcttcaaa aacgcgcgca tcgttcgcct gtctggctcc
     1801 agatggcctg ggagtagggc aaatggttta aaaaggactt accattggtc ccgatgtggg
     1861 attcccgcgt cgatcgagac aggcagggcc agatctacag ccccaggtgg gtcaatgaac
     1921 ggtcgagcat cagcagttgc cacaggagcc gcgaatggtc gctgcggccg gcgatgtggg
     1981 cttcggccat gcctgccact gccgccggat cgagccagcc ggtgcgcgcg agcgtggtgc
     2041 tgccggcaat ggcgcgggcg gcagtggcga gcgggccgcg cagccattgg gcgatggggg
     2101 tgacgaagcc ttgcttgggc cgcatcagga tatcctcggg cagatagcgg cgcatggcct
     2161 gtttcatcag ccacttgccc tgggtgccgc gaatgcgcag cccttccggg agccgggcgg
     2221 cgaattcgac cagccgatgg tcgagcagcg gttcgcgtgc ttcgaggctc accgccatcg
     2281 acgtgcggtc aaccttggtc aggatatcac ccggcagcca gaaggtcagg tcggcatatt
     2341 gcgctcggtc gagcgccgag cgcgccggtg cgcgggtcat ggcttccagc aaaggctgtt
     2401 cggcccgata atcgcggcgc aggcgctgga attccggggt gtagagccgg tcccgctggt
     2461 ccggcgtggt cacggccagg gcgcgggcat aggcggcatc ggcaggttcg gaaagcgcca
     2521 ggagggtagt cttggcccgc agcggacggg gggcccagtc ggccttgggc cagatcgcgc
     2581 cgaggccgcc gaacagcggc gcgcgcaagc cctggggcag cagctgtcgc agccggtcct
     2641 cgtggcggtg gaaccgctgg cgccgataac cggccagcgc ctcgtctgcc ccatcaccgg
     2701 agagggcgac cgtcaccgtc tcgcgcgcca gctggcagac ccgccaggtc ggcagggccg
     2761 aggcatcggc aaagggctcg tcgaacatgg ccgcgagttg cccgatggcg ccgaaatcgt
     2821 caggagaaac ggcgcgggcg tgatggtcgg tggcaaagcg gttggcgacc tggcgggcat
     2881 aggcgctctc gtccagcccc gcctcggcaa agccgatcga gcaggtcttg accgggctgc
     2941 ccgacatctc ggccatcagg gcgacgacgc tggagctgtc gaccccgccc gacaggaagg
     3001 cgccgagcgg gacatcggcg gccatgcgcg agcggaccgc ctcgcgcaga tgatggagca
     3061 gctcggcctc gagatcggcg gcgctctcgc gccggcggtc ggcgaacgac ggttcccacc
     3121 agcgcaccgg tgccggcagc ggcgaaccgt ggcggagcag caggtaatgc ccggcaggca
     3181 gcttgtggac gcctttgagg atcgcgcggt gatcgggcac gtagccccag gcgagatagt
     3241 cctccaccgc cagcggatcg atctcgcggc gcagcagggg atgggtgagc aggcccttga
     3301 gctcggaggc gaaggccagg ctgccatcgc tgagcgcggc gtagtggagc ggcttgacgc
     3361 ccagccggtc gcgagcgagg aacagcgtgc gctggcccat gtcgtagagc gcaaaggcga
     3421 acatgccatt gagccgggca aggcaagcgg ggccccattg ctgccaggcg gcgaggatga
     3481 cctcggtgtc gccatcggtg cggaacacgg caccccgcgc ggccagttcg gtgcgcagtt
     3541 cgcggtagtt gtagatctcg ccgttgaaga cgatcactgc ccggccatcg gccgcgtgca
     3601 tcggctgggg ggatccggca aggtcgatga tcgagaggcg gcggtggccc aggcccacgc
     3661 ccggcgcggt ccacaccccc gccccgtcgg ggccgcgatg gaccatggca tcgcacatcc
     3721 gctcgacccg tgaggggtcg accggcttgg gcgtggtcag gtggaaaagg ccggcaatgc
     3781 cgcacatgat tcgcggttgc ctagcggagc ccggccaggc ggtccatcca cggtccgagc
     3841 ggaccggccg accggcggaa cgcctcgagg gtggcagcct gggccgcttc gggttgctgg
     3901 tcggttgaaa ggatgagcag catggtcggc gccggacgca ggatcaggcg gtcgcgaatg
     3961 ttggccagct tgaggcgggc attgctgccg gtaagcaggc tgtcggtacg gtaataggtc
     4021 tcggcgagcc ggggcacggt gctcttgcca agcaggcggt cggccttgcc gtcggcgagt
     4081 gcaggcccgg gcgcgcgcca gtcccatccg ctttcgggga ccagcgcccc ctgcccgaac
     4141 ccgccggcct cccggccttc ggactggctg gaatataggg cgaagaacac gtcgaccgtg
     4201 cgcccctcgc catcggcata acggccgagc aggcgatgat tggcgccgct ggcacgcggt
     4261 tcccaccaca acgccggcgt gtagccgacc cggtgccagc ccggcacctc cggcaggaac
     4321 acctgccgcg gcacgggcgc ctcaagccgg tcggcagccc gcgaccaggc ctg
//
LOCUS       DATNBV010000390         3986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1507795,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000390 DATNBV010000000
VERSION     DATNBV010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507795"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VN627_09385"
     CDS             <1..306
                     /locus_tag="VN627_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03508.1"
                     /translation="LITENIDAVERAVNRLNAPPGKYVNLEEPAIDSVRASDDCAANQ
                     RKQRRARYPPGQPREQRLPHSGGPYKTHIAPDIECFGRPTFANLVSRESIIGRASGI"
     gene            475..2565
                     /locus_tag="VN627_09390"
     CDS             475..2565
                     /locus_tag="VN627_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXD03509.1"
                     /translation="MIIGVDIGGTFTDCTVVTGSGQAYSGKSPTTPQDLSIGFFESIA
                     SAAGALDLPLSELLQQTTRICHGTTTGLNALVTGAGARTLLLTSAGHADALSIMNNRG
                     RTQGASVRETLDWSISSHPDPIVPRDLVIEVNERTDSFGAVVCPLRDEEVARVLDLVA
                     EAKPEAIALCCLWSFANPEHEIRLRSALRERFPEVPISCSFEVAPRIGLYPRMVTTVM
                     NAMLVPLMTAYVARIEERAAANGFDGGIFFVQNEGGLVPASEAAAFPIMTLKSGPVAG
                     VVGAGIVGSQLDDPDIIVADMGGTTFDVGVIRNGLPGRIDESVMRRQQLHLRSVDVDS
                     VGAGGGSIAWVDERTKTLRVGPRSAGARPGPICYGNGGTEVTVTDADLVLGVLDDQRP
                     LAGGLKLDRIAAEAGLARLGAKLGMDAVRCAAGIVEVVDSLMEDLIRRTTVQRGLDPR
                     EFSLWVYGGASGAHAGLFSRHLQMKRVVLAMGNVASVWSAAGCALLRQRREFSTSIYM
                     PPAWDMDLLAGHFADLQARSERYAEQIGLEAGSFTVRRSATMKYGMQVHEIEVDGQPG
                     MIDAAWGVALKDDFEATYEQVFGAGSGYSGTDVIITALRTVVEVEQAPMVLTRQSAAT
                     NQASQPTTRPVFWRELDDWRETAVWQGDELPTDVEIEGPGIVEYPHTTLVIRPDQKFF
                     RDQQGNIILELGRA"
     gene            2570..>3986
                     /locus_tag="VN627_09395"
     CDS             2570..>3986
                     /locus_tag="VN627_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HXD03510.1"
                     /translation="MSTTTHNPDIQARKTAAAQFGYLNPVLSQVEDHITLHTDQDDSL
                     DPVTYEIIRSKLGNINIDHGETIRRISGSMPVAEGNDYNASIATELGEGTAFGAHNWF
                     FAGYADLAIRWVLEFRAHTVGVNDGDVFIHNDPWVGTNHQNDAAVMAPVFWDGKLFAW
                     IYNSAHQQDMGGPTPGSMTPLARTVFEESDCIPPVKIVANGVLQDDILDFWTRRTRAP
                     TTSSLEVKSQLAGAKLARDRLLEVISRYGPAKVKRAMHKTIADAAQIVGQRLRQIPDG
                     IWADERYIGVTGADNWTTPYRLCIQFEKRGDRLRITNRGTSPTGPGVNFTAGAFRGCV
                     LNGLFHVLGFDIDLCGAGLLRQLDFELTPGLINAATYPAAVSSSVGTMSTVVQSYCLG
                     SKMVSGVPGLSSHSAASHSLHTMSAAFNMDGIDRFGNFFKTTILDLIAGGSGGYSDHD
                     AIDHSGNAAGSSHPIPDIELVE"
BASE COUNT          729 a         1283 c         1298 g          676 t
ORIGIN      
        1 ttaattacgg agaatatcga tgcggtggag agggccgtca atcgactaaa cgccccgccc
       61 ggtaaatacg tcaatctgga agagccggcg atagatagtg tccgcgcctc ggatgattgc
      121 gccgcaaatc aaagaaaaca acgccgcgcg cgctatccgc cgggccaacc gcgggagcag
      181 cgcctgcccc actccggcgg tccatacaaa acgcatattg ctccagacat agaatgtttt
      241 ggacggccca ctttcgcgaa cttagtctct cgagaaagca taatcgggag agcgtctggc
      301 atctgacatc caggtttcca gcagcgcgag gggctaagtc gcggttccgg atggacccgc
      361 gggcggtgtg ctagatgccg ccctctgata ctccagaatt aaataaatcc ggggggatat
      421 ccctcacata ttcataaccg tgacttaccg cggaatgcag ggagctatag caagttgatt
      481 atcggagtag acatcggagg gacgttcacg gattgcacgg tagtgacggg cagcgggcag
      541 gcctattccg gcaagtcgcc cacgaccccc caagaccttt cgatcggctt ctttgaatca
      601 atcgccagcg cagccggcgc gctcgacctg ccgttgagcg aactgctgca gcaaaccacg
      661 cgtatctgcc atggcacgac gaccggtttg aacgcattgg tcaccggcgc cggggcaagg
      721 acactgctgc ttaccagcgc cggccatgcg gatgcgctga gcatcatgaa caaccggggg
      781 cgcacgcaag gggcatcggt ccgcgaaacg ctggactggt cgatcagttc gcatcccgat
      841 ccgatcgtgc cccgggacct cgtgatcgag gtcaacgagc ggaccgacag cttcggcgcc
      901 gtcgtctgcc cgctgcggga cgaggaagtc gcgcgtgtgc tggacctcgt cgccgaggca
      961 aagccggaag ccatcgcgct gtgctgcctg tggagctttg ccaacccgga gcatgaaatt
     1021 cgcttgcgct cggccttgcg tgagcgcttc cccgaggtcc ccatatcctg cagtttcgag
     1081 gttgccccgc ggatcgggct ctatccgcgc atggtcacca ccgtgatgaa cgccatgctg
     1141 gtgccgctga tgacggccta tgtggcgcgg atcgaggagc gggcggcggc caacggcttc
     1201 gatggcggca tcttcttcgt acagaacgag ggcgggctgg tccccgcctc cgaagccgcg
     1261 gcattcccga tcatgaccct gaagtccggg ccggtggcgg gcgtggtcgg ggcgggcatt
     1321 gtcggcagcc agttggacga tcccgatatc atcgttgccg acatgggcgg gaccacgttc
     1381 gacgtcggcg tgatccgcaa cggtctgccc ggccggatcg acgaaagcgt gatgcggcgc
     1441 cagcagctgc acctgcgctc ggtggacgtg gattccgtgg gcgcgggcgg cggcagcatc
     1501 gcctgggtcg acgagcgcac gaaaacgttg cgggtggggc cgcgcagcgc gggggcccgg
     1561 cccggcccga tctgctatgg caacggcggg accgaagtaa ccgtgacgga cgccgacctg
     1621 gtccttggcg tgctcgacga tcagcgcccc ctggcgggcg gcctcaagct cgaccggata
     1681 gccgccgaag cagggctggc gcggcttggg gcgaagctgg gcatggacgc ggtgcgctgc
     1741 gccgcgggca tcgtcgaggt ggtcgacagc ctgatggagg acctgatccg gcgcaccacg
     1801 gtccagcgcg gactcgatcc ccgggagttc agcctgtggg tttacggcgg cgcctcgggc
     1861 gcccacgccg ggctgttcag ccggcacctg cagatgaagc gggtcgtcct ggcgatgggc
     1921 aacgtcgcct cggtctggtc tgccgccggc tgcgccctgc tgcggcaaag gcgcgagttt
     1981 tcgacgtcga tctacatgcc gccggcctgg gacatggacc tgcttgccgg gcacttcgcc
     2041 gatcttcagg cgcgctcgga gcgctatgcc gagcagatcg ggctggaggc cggcagcttc
     2101 acggtgcgcc gatcggccac gatgaaatac ggcatgcagg tgcacgagat cgaggtcgac
     2161 ggccagccgg gcatgatcga cgccgcctgg ggcgtggccc tgaaggacga cttcgaggcg
     2221 acttacgagc aggtattcgg cgccggctcc ggctattccg ggacggatgt catcatcacc
     2281 gccttgcgga cggtcgtgga ggtcgaacag gcgccgatgg tgctgacccg gcagtcggcg
     2341 gccaccaacc aggccagtca gcccacgacg cgcccggtct tctggcgcga actggatgac
     2401 tggcgcgaaa ccgccgtctg gcagggcgac gaacttccca ccgacgtgga gatcgagggc
     2461 cccggcattg tcgaataccc ccatacaact cttgtgatca ggcccgacca gaagttcttc
     2521 cgggatcagc agggcaatat catcctggaa ctcggcaggg catgacgcga tgagcaccac
     2581 cactcacaat ccggacatcc aggcccgaaa gaccgcggcc gcccagttcg gctacctgaa
     2641 tcccgtgctg tcgcaggtgg aggaccatat taccctccac accgatcagg acgacagcct
     2701 ggacccggtt acctacgaga tcatcaggtc caagctcggc aacatcaata tcgatcacgg
     2761 cgagaccatc cggcgcatct ccggctcgat gccggtggcc gagggcaacg actacaatgc
     2821 gtcgatcgcc accgaactcg gcgaaggaac cgccttcggc gcgcacaact ggtttttcgc
     2881 cggctacgcg gatctggcca tacgctgggt actggagttc cgtgcgcaca cggtgggggt
     2941 gaacgacggc gacgtgttca tccataacga tccgtgggtc ggaaccaatc accagaacga
     3001 tgcggcggtc atggccccgg tgttctggga cggcaagctg ttcgcctgga tctacaacag
     3061 cgcccaccag caggacatgg gcggcccgac tccggggtcg atgaccccgc tcgcgcgcac
     3121 cgtgttcgag gagtccgact gcatcccgcc ggtgaagatc gtcgccaatg gcgttcttca
     3181 ggacgatatc ctggatttct ggacccgccg cacccgggcg ccgacgacct cttcgctgga
     3241 ggtcaagtca cagctggccg gggcaaagct ggcgcgcgac cgcctgctcg aagtcatctc
     3301 gcggtatggc ccggcgaagg tcaagcgggc catgcacaag acgatcgccg acgccgcgca
     3361 gatcgtcggg cagcgcttgc ggcagatccc cgacggcatc tgggccgacg aacgctatat
     3421 cggcgtgacc ggggccgaca actggaccac gccctatcgc ctgtgcatcc agttcgagaa
     3481 gcgcggcgat cggctgcgga tcaccaaccg gggaaccagc ccaaccggac cgggcgtgaa
     3541 cttcaccgcc ggggcattcc gtggatgcgt tctcaacggc ctgttccacg tcctcggctt
     3601 cgacatcgac ctgtgcggcg cggggctgct gcggcagctc gatttcgagc tgacccccgg
     3661 cctgatcaat gccgcgacct atcccgcggc tgtatcctcg tctgtgggaa cgatgtcgac
     3721 ggtggtgcag tcgtattgcc tgggctcgaa gatggtctcg ggcgttccgg gtctctcatc
     3781 ccattcggcc gcctcgcatt cgcttcatac catgtcagcc gccttcaaca tggatggcat
     3841 cgaccgcttc ggcaacttct tcaagaccac cattctcgac ctgatagccg gcggctcggg
     3901 cggctattcc gaccacgacg cgatcgatca ctcggggaac gccgccggca gcagccaccc
     3961 gatcccggat atcgagctgg tggagc
//
LOCUS       DATNBV010000391         4243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_861183,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000391 DATNBV010000000
VERSION     DATNBV010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861183"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VN627_09400"
     CDS             complement(<1..323)
                     /locus_tag="VN627_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HXD03511.1"
                     /translation="MRDAYRQAFDESTTNRDAFWSRAAAAVDWFTPPEAVHDDARGWF
                     AGGTLNTCHNALDRHVAAGRGDAAALIYESPVTGTTRTFSYDQLLEEVGRVAAMLVRL
                     GVAKG"
     gene            complement(379..2142)
                     /locus_tag="VN627_09405"
     CDS             complement(379..2142)
                     /locus_tag="VN627_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD03512.1"
                     /translation="MSQTAIPDDVSGLPKHHQATHSEKLIITASSLGTVFEWYDFYLY
                     GLLTAIIAAKFLTGLNPTTSFIMALLVFAAGFIVRPFGALVFGKIGDTFGRRYTFIIT
                     LLVMGLSTFLVGCLPTYASVGVAAPIMLVVLRMCQGLALGGEYGGAATYVAEHAPNNK
                     RGFYTSWIQITATAGLAMALLIVILVRSPVTGVGEDAFKDWGWRVPYLLSGLFLAVGL
                     WLRLKLHESPVFQKMKAEGTSSKRPLSEAFGEWKNLKIVLIAFFGAIAGQAVVWYTGQ
                     LYAMYFLEKMLKVDGITANSLIIVALLLATPFFLVFGWLSDKIGRKPIILTGCALAAA
                     SLFPVFHALTDAANPALARAQATAPVTVTAYPGECSSQFDPVGTNKFDQTSCDIVKNA
                     LAKAAVNYTNVAAPAGTIASIAIGSETIAAPDPKAVSGDEKKAAIAAFTAKVAGAPAV
                     AAKAAEGGKPAVAAKEAVVGELSKVGYPAKADPAQINKPRVIALLWYLVLLVTMVYGP
                     IAAMLVELFPSRIRYSSMSLPYHIGNGWLGGLLPAIGFAMVAANGNIYHGFWYPVVVA
                     AATCVIGLIFLPETFRRGIDD"
     gene            complement(2167..3663)
                     /locus_tag="VN627_09410"
     CDS             complement(2167..3663)
                     /locus_tag="VN627_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HXD03513.1"
                     /translation="MSGLRTRTIAILMGASALALPSLAHAATARERALEARLEKIEAQ
                     MAAMQGELAAARGAQAEAVAAANAAATSAGQLASAASAQAQAATTKVAALEARPTAPA
                     EGFRSGSTTIKLGGYLKMVASHTHFSNGSVATNTLGRDFYLPQTIPTTVGGHDFDGED
                     FTAKQTRLWLNLDTQVAGHVVKGYLESDFQVTASAVQNVTAGGSQRTTNGYTLGLRRA
                     YVQVDRFTLGQDWTTFQYTGALPESTDYVGGAEGTVFVRQPLVRYAAPLSKQVTLAVA
                     VENPESALVTAGATTTLESGTDHLPDFTARLTYAGKRGEVALSGLVRQVRGESTGSAL
                     VNNVSTAGATGQGVTATGFGASIGGKLFLNEKKTSDIRFIATYGQDIGRYVGLNFAAD
                     AVYDPATNRLTDVKVLAALASAHLTVAPQWRVNLMGSFQHVTYDRSLTLASLAGLNKS
                     AWSAAANLFWSPVKQIDLGLEYRHGSRELVSGASGSVDRVEFAAKYSF"
     gene            3834..>4243
                     /locus_tag="VN627_09415"
     CDS             3834..>4243
                     /locus_tag="VN627_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HXD03514.1"
                     /translation="MAETIYPVPADWAGSARFDEAGYAAHYRRSVDQPEAFWAEMAQR
                     LDWIRPFSVVKQTSFAQDDFGIRWFGDGTLNISANCLDRHLAERGDSVAILWEPDHPD
                     TPDRRITYRELHEAVCRFANLLKARGVRKGDRVTL"
BASE COUNT          699 a         1345 c         1488 g          711 t
ORIGIN      
        1 tcgcccttgg ccacgccaag gcggacgagc atggcggcaa cgcggccgac ttcctcgagc
       61 agctggtcgt aggaaaaggt gcgggtcgtg ccggtgaccg ggctttcgta aatcagcgcg
      121 gcggcatcgc ctcgccccgc ggcgacgtga cgatcgagcg cattgtggca ggtgttgagc
      181 gtgccgccgg cgaaccagcc gcgggcgtcg tcgtgcacgg cctcgggcgg ggtgaaccag
      241 tccaccgccg cggccgcgcg cgaccagaag gcatcgcggt tggtggtgct ttcgtcgaaa
      301 gcctggcggt aggcgtctct catggtgtcc tctcaggtca ggagccggcg ccgcccgaaa
      361 gcggcgccgg tccggtcatc agtcgtcgat gccgcggcgg aaggtctcgg gcaggaagat
      421 cagcccgatg acgcaggtcg ccgccgcgac caccacgggg taccagaacc catggtagat
      481 gttgccgttg gccgcgacca tggcaaagcc gatcgccggc agcagtccac cgagccagcc
      541 gttgccgatg tggtagggca ggctcatcga ggagtagcgg atgcggctgg ggaacagttc
      601 aaccagcatg gccgcgatcg ggccgtagac catcgtcacc agcagcacga ggtaccacag
      661 gagggcgatg acccggggct tgttgatctg ggccggatcg gccttggccg gatagccgac
      721 cttcgacagt tcgccgacaa ccgcttcctt ggccgcgacc gcgggcttgc cgccctcagc
      781 cgccttggcg gcaactgccg gagcgcccgc gaccttggcg gtgaaggcgg cgatggcggc
      841 cttcttctcg tcaccgctca ccgccttcgg atccggcgcg gcgatggttt cgctgccgat
      901 cgcgatcgag gcgatcgtcc cggccggcgc ggcgacattg gtgtagttca ccgccgcctt
      961 ggccaaagcg ttcttgacga tatcgcagct ggtctggtcg aacttgttgg taccgacggg
     1021 atcgaactgc gacgagcatt cgcccgggta agcggtgacg gtcaccggtg ccgtggcctg
     1081 ggccctggca agcgccggat tggccgcatc ggtcagcgcg tggaacacgg ggaacaggct
     1141 cgccgcggcc agggcgcagc cggtgaggat gatcggcttg cggccgatct tgtcggacag
     1201 ccagccgaag accaggaaga acggcgttgc cagcagcagg gcgacgatga tcaggctgtt
     1261 ggcggtgatg ccatcgacct tgagcatctt ctcgaggaag tacatcgcgt agagctggcc
     1321 ggtgtaccag accacggcct ggccggcgat ggcgccgaag aaggcgatca ggacgatctt
     1381 gaggttcttc cattcgccga aggcttccga cagcggccgc ttcgacgagg tgccttcggc
     1441 cttcatcttc tggaacaccg ggctctcgtg gagcttgagt cgcagccaga ggccgacggc
     1501 gaggaacagg ccgctcagca ggtagggcac gcgccagccc cagtccttga acgcgtcctc
     1561 gcccacgccg gtcaccggcg agcggacaag gatcacgatc agcagcgcca tggcgaggcc
     1621 cgccgtggcg gtgatctgga tccagctggt gtagaacccg cgcttgttgt tgggcgcgtg
     1681 ctcggcgacg taggtcgccg cgccgccata ttcgccgcca agggcaaggc cctggcacat
     1741 gcgcaggacg acgagcatga tcggcgcggc cacgccgacg ctggcatagg tcggcaggca
     1801 gccgacgagg aaggtcgaca ggcccatcac cagcagggtg atgatgaagg tgtagcgccg
     1861 cccgaaggtg tcaccgatct tgccgaagac cagcgcgccg aacgggcgca cgatgaaccc
     1921 ggcggcgaag accagcaggg ccatgatgaa cgaggttgtg gggttgagcc cggtcaggaa
     1981 cttggccgcg atgatcgcgg tcaacagacc ataaaggtag aagtcgtacc actcgaacac
     2041 cgtgccgagc gacgaagcgg tgatgatcag cttttcgcta tgggtggcct ggtggtgttt
     2101 gggcaggccg gatacgtcat ccggtatggc agtctggctc atggcccctc tccttattgt
     2161 ggtttcttag aaactgtatt tggcggcaaa ttcaacccgg tcgaccgagc cggaagcccc
     2221 gctcaccagt tcgcgcgaac cgtggcggta ttcgaggccg agatcgatct gcttgaccgg
     2281 cgaccagaac aggttcgccg ccgcgctcca tgccgacttg ttgagcccgg caaggctcgc
     2341 cagggtgagc gagcgatcgt aggtgacgtg ctggaagctg cccatcaggt tgacccgcca
     2401 ttgcggggcg accgtcaggt gggccgaggc cagggcggcg agcaccttga cgtcggtcag
     2461 gcggttggtc gccggatcgt agaccgcatc ggccgcgaag ttcaggccga cgtatcggcc
     2521 aatgtcctgc ccgtaggtgg cgatgaagcg gatatcgctg gtctttttct cattgaggaa
     2581 cagcttgccg ccgatcgatg cgccgaagcc ggtggcagtc acgccttggc cggtcgcgcc
     2641 ggcggtgctg acattgttga ccagcgccga accggtgctc tccccgcgca cctgccgcac
     2701 caggcccgac agcgccacct cgccccgctt gccggcatag gtcagccggg cggtgaaatc
     2761 gggcagatgg tcggtgccgc tttcgagcgt ggtggtggcc ccggccgtga cgagcgcgct
     2821 ttccgggttt tccacggcga cggctagggt cacctgcttc gacagcggcg cggcatagcg
     2881 gaccagcggc tggcggacga agaccgtgcc ctccgcgccg ccgacgtagt cggtcgattc
     2941 gggcagggcc ccggtgtact ggaaggtcgt ccagtcctgc ccgagagtga agcggtccac
     3001 ctggacgtag gcgcggcgca ggccgagcgt atagccgttg gtcgtgcgct gcgagccgcc
     3061 ggcggtgacg ttctggaccg cgctggcggt gacctggaaa tcgctttcga ggtagccctt
     3121 gaccacgtgg ccggcgacct gcgtatcgag gttgagccac aggcgggtct gcttggcggt
     3181 gaagtcctca ccatcgaagt cgtgaccgcc gaccgtggtc gggatcgtct gcggcagata
     3241 gaaatcgcgg cccagcgtgt tggtggcgac cgatccgttg gagaaatggg tgtggctggc
     3301 aaccatcttg aggtagccgc caagcttgat cgtggtgctg cccgagcgga agccttccgc
     3361 cggcgcggtt ggccgggctt caagggcggc gaccttggtc gtcgccgcct gagcctgggc
     3421 cgaggcagcg ctggccaact ggccggccga ggtggcggcg gcattggcgg cggcaacggc
     3481 ttctgcctgg gcccctcgcg cggcggcgag ttcgccctgc atcgcggcca tctgggcctc
     3541 gatcttctcc agccgcgcct caagggccct ttcgcgggcc gtggcggcat gagccagcga
     3601 gggcagcgcg agcgcgctcg cccccatgag aattgcgata gtacgggtcc gcagtccgga
     3661 catgccccct ctccttttca tatctgattt gggcgagggt gatggtgccg ccttgccgca
     3721 ttcctttgca gccggacctt ggtcgaacgg ctacgacctt ggtctatgtc ccatgcggca
     3781 gggcgctcag gctaacgtcg ccggcaaacc agacatgaga ggacaagccc gccatggccg
     3841 agaccatcta tcccgtaccc gccgattggg ccgggtccgc ccgcttcgac gaggccggat
     3901 atgccgccca ctatcgccgc tcggtcgacc agcccgaggc cttctgggcc gagatggcgc
     3961 agcggctgga ctggatccgg ccgttcagcg tggtcaagca gaccagcttt gcgcaagacg
     4021 atttcggcat ccgctggttc ggtgacggga ccctcaacat ttcggccaac tgccttgacc
     4081 ggcacctcgc cgagcgcggc gacagcgtgg cgatcctgtg ggaaccggac catcccgaca
     4141 cgcccgaccg ccgcataacc taccgcgagc tgcacgaggc ggtctgccgc ttcgccaacc
     4201 tgctgaaggc gcgtggggta aggaagggcg accgcgtaac gct
//
LOCUS       DATNBV010000392         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3723230,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000392 DATNBV010000000
VERSION     DATNBV010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3723230"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /gene="fusA"
                     /locus_tag="VN627_09420"
     CDS             <1..861
                     /gene="fusA"
                     /locus_tag="VN627_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042194.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXD03515.1"
                     /translation="PVIEVAVEPKSKADQEKMGVALNRLAQEDPSFRVSSDQESGQTI
                     IKGMGELHLEIIVDRMRREFKVEANVGQPQVAYRESITKKIEWDYVHKKQTGGSGQYA
                     KVKIRFEPGAPGSGFVFENDVVGGSVPKEYVPGVEKGLTNAKETGVIAGFPVIDLKCT
                     LIDGDYHDVDSNVMTFDIAARACFREAMPKAGPRLLEPMMKVEVVTPQEYMGDVIGDL
                     NSRRGQVQGMDSRGNAQVISAMVPLANMFGYVSTLRSMTQGRAQYTMHFDHYEQVPPA
                     IADEVRAKMA"
     gene            890..2080
                     /gene="tuf"
                     /locus_tag="VN627_09425"
     CDS             890..2080
                     /gene="tuf"
                     /locus_tag="VN627_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HXD03516.1"
                     /translation="MAKAKFERTKPHCNVGTIGHVDHGKTSLTAAITKVLAETGGATY
                     TAYDQIDKAPEERARGITISTAHVEYETKARHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPALVVYMNKCDMVDDPELLDLVELEVRELLSS
                     YKFPGDKIPVIRGSALMALEDKNPELGKQSILKLMQAVDESIPQPDRPKDRPFLMPIE
                     DVFSISGRGTVVTGRIERGIVKVGEEVEIIGLKPTVKTVVTGVEMFRKLLDSGEAGDN
                     IGALLRGTKREEVERGQVLAKPGSITPHTKFKAEAYVLTKEEGGRHTPFFSNYRPQFY
                     FRTTDVTGTVVLPAGTEMVMPGDNISMEVHLIAPIAMDEGLRFAIREGGRTVGAGVVA
                     SIIE"
BASE COUNT          428 a          602 c          726 g          325 t
ORIGIN      
        1 ccggtgatcg aggtcgcggt cgagccgaaa tccaaggccg accaggaaaa gatgggcgtc
       61 gcgctcaatc gcctggcgca ggaggaccca tccttccgcg ttagctccga tcaggaatcc
      121 ggccagacca tcatcaaagg catgggcgag ctccacctcg agatcatcgt cgaccgcatg
      181 cggcgcgagt tcaaggtcga ggccaatgtc ggccagccgc aggttgccta tcgtgaatcg
      241 atcaccaaga agatcgagtg ggactacgtc cacaagaagc agaccggcgg ctcgggccag
      301 tatgcgaagg tcaagatccg cttcgaaccg ggcgctcccg gcagtggttt cgtgttcgag
      361 aatgatgtgg tcggcggctc ggtgccgaag gaatacgtgc ccggcgtcga aaagggcctg
      421 accaatgcga aggaaacggg cgtcatcgcc ggcttcccgg tcatcgatct caagtgcacg
      481 ctgatcgacg gcgactacca tgacgtcgat tccaatgtca tgacgttcga catcgcggcg
      541 cgcgcctgtt tccgcgaggc gatgcccaag gccggcccgc ggctgctgga gccgatgatg
      601 aaggtcgagg tcgtgacgcc ccaggaatac atgggcgacg tgatcggcga tttgaacagc
      661 cggcgcggcc aggtgcaggg catggacagc cgcggcaacg cgcaggtgat cagcgcgatg
      721 gtgccgctgg ccaacatgtt cggttacgtg agcacgctgc ggtccatgac ccagggccgt
      781 gcgcaataca ccatgcattt cgatcactat gaacaagtgc cgccggcgat cgcggatgaa
      841 gtccgcgcca agatggctta gcgcgggcga aaggtaaggg agcagaaaaa tggcgaaggc
      901 gaagtttgag cggacgaagc cgcactgcaa tgtcgggacg attggtcacg ttgaccatgg
      961 caagacgtcg ttgacggcgg cgatcacgaa ggtattggcc gagacgggtg gtgcgaccta
     1021 tacggcctac gaccagatcg acaaggctcc ggaggagcgg gcgcgcggca tcacgatctc
     1081 gaccgcgcat gtggagtatg agaccaaggc gcggcactac gcccacgtcg actgcccggg
     1141 ccatgcggac tatgtgaaga acatgatcac cggcgcggcg cagatggacg gcgcgatcct
     1201 ggtggtcagt gccgccgacg gcccgatgcc gcagacccgc gagcacatcc tgctggcgcg
     1261 ccaggtcggc gttccggcgc tggtggtcta catgaacaag tgcgacatgg tggacgatcc
     1321 ggagcttctg gacctggtcg agctggaagt gcgggaattg ctctcgtcct ataagttccc
     1381 gggcgacaag atcccggtca tccgcggctc ggcgctgatg gcgctggaag acaagaaccc
     1441 ggagctgggc aagcaatcga tcctgaagct gatgcaggcg gtggatgaga gcatcccgca
     1501 gccggaccgc ccgaaggacc gcccgttcct gatgccgatc gaggacgtgt tctcgatctc
     1561 tggccgcggc acggtggtga cggggcggat cgagcgcggg atcgtgaagg tcggcgagga
     1621 agtggagatc atcggcctga agccgacggt gaagacggtg gtgacggggg tcgagatgtt
     1681 ccgcaagctg ctggacagcg gcgaggcggg cgacaatatc ggcgccttgc tgcgcggcac
     1741 caagcgcgag gaagtggagc gcgggcaggt cctggccaag cccggctcga tcacgccgca
     1801 caccaagttc aaggcggaag cctatgtgct gaccaaggaa gagggcgggc gtcacacgcc
     1861 gttcttctcg aactaccgtc cgcagttcta cttccgcacg accgacgtga cgggcacggt
     1921 ggtgctgccg gcgggcacgg aaatggtgat gccgggcgac aacatctcga tggaagtgca
     1981 cctgatcgcg ccgatcgcga tggacgaggg cctgcgcttc gcgatccgcg aaggcggccg
     2041 aaccgtcggc gccggcgtcg tcgccagcat catagagtaa g
//
LOCUS       DATNBV010000393         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3724326,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000393 DATNBV010000000
VERSION     DATNBV010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3724326"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..659
                     /locus_tag="VN627_09430"
                     /pseudo
     CDS             60..659
                     /locus_tag="VN627_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            656..1279
                     /locus_tag="VN627_09435"
     CDS             656..1279
                     /locus_tag="VN627_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXD03517.1"
                     /translation="MRIALLHGWGMDRNTWDAVIPLLPEFVCQADDRGYFGPAEAASP
                     ADLVVTHSLGALRALHTPPEGCRALVVINGFDRFGQVPDFPDGVPGIATTRMAERLEA
                     DAQAELTRFRLRIGAGAPRPIADRAALLADLDFLRSFDGRGLWQGPLLLLGGDADLVV
                     RPAHQAACFADRPEALRSTIPGGGHLLPVTAPDACAAAIRQMAGLIG"
     gene            1276..2064
                     /locus_tag="VN627_09440"
     CDS             1276..2064
                     /locus_tag="VN627_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXD03518.1"
                     /translation="MTLPESLARRRRIGAAFAAAQDYDRHARVQHEVAVGLAQRIAAL
                     PLPPDARVLEIGCGTGFLTDELVRREVSGALLVTDLAPAMIQRCRARLGDEVGRAFRV
                     LDGERGERPAEAPFDLISSSLAFQWFENLPAALARLAGWLAPGGTLAFTTLAEGTFAE
                     WREAHAALGLASGTPHFPSAEQLRRMAPAGFQADISVATHAEPQGSAPAFLKALKAIG
                     AGTAAAAHQPLPPGALRQVMARFDAGGAVATYEVAICLLRREAA"
BASE COUNT          245 a          754 c          755 g          329 t
ORIGIN      
        1 tcgagcgcca tggcgcggcg gcaccggcgc ggctgatcct ggtcgaaagc gtctattcga
       61 tggacggcga ccgggccgat attcccgccc tggccgccct cgcccgccag catgatgccg
      121 tgctgatggt cgatgaggcc catgccaccg gcgtgctcgg ccagggcggc gcgggcctgt
      181 gcgccgggat cgacggggtg gaggtggtca tgggcacctt ctccaaggcc ttcggctgat
      241 tcggcgccta tgtcgccgga tcggcggtgc tgcgcgactg gctggtcaac gcagcctcgg
      301 gcttcatctt ctccaccgcc ccgcccccgg ccatgctcgg cgcgatcgac gccgcgctcg
      361 atctggtgcc gggcatggac agtgagcggg cccatcttgc ctgtctgggt gagcgcttgc
      421 gcgcggggct tgccgggcgc ggcttcgata cggctggctc ggcctcgcag atcgtgccgg
      481 cgatcatcgg cagcgaggcg gatgccctgg cattgagcga gcggctcgcc acgcggggca
      541 tgcttgccgc cgcaatccgc ccacccacgg tgcctgccgg caccagccgg ctgcggctgg
      601 cgctgcgcgc gacccatgcc gaggccgatg tcgacgcgct gctcggggcg ctggcatgag
      661 gatcgccctg ctccacggct ggggaatgga ccgcaatacg tgggatgcgg tgatcccgct
      721 gctgcccgag ttcgtctgcc aagccgatga tcgcggctat ttcggcccgg cggaggcggc
      781 cagcccggcc gatctcgtgg tgacccacag cctcggcgcc ctgcgcgcgc tgcacacgcc
      841 gccggaaggg tgccgggcgc tcgtcgtgat caacgggttt gaccggtttg gccaagtgcc
      901 cgactttccc gacggggtcc ccggtattgc caccacgcgc atggccgagc ggctcgaggc
      961 agatgcccag gccgaattga cgcggttccg cctgcgcatc ggcgcgggtg cgccccgccc
     1021 gatcgccgac cgggcggccc tgctggccga tcttgatttc ctccgttcgt tcgacgggcg
     1081 cggcctctgg caggggccgc tgctgctgct gggcggcgac gcggatctgg tggtgcgtcc
     1141 cgctcaccag gccgcctgct tcgccgaccg ccccgaggcc ttgcgcagca cgatccccgg
     1201 tggggggcac ctgctgcccg tgacggcgcc agacgcctgc gccgccgcga tccgccagat
     1261 ggcaggactg atcggatgac gctgccggaa agccttgccc gccgcaggcg gatcggcgcc
     1321 gcctttgccg ccgcgcagga ctatgaccgc cacgcacgcg tgcagcatga ggtggccgtc
     1381 ggccttgccc agcggatcgc tgccctgccg ctaccacctg acgcgcgggt gctggagatc
     1441 ggctgtggga cgggctttct caccgacgag ctggtccgcc gcgaggtgag cggggcactg
     1501 ctggtgaccg accttgcccc ggcgatgatc cagcgctgcc gcgcccgtct gggcgacgaa
     1561 gtgggccgcg cctttcgcgt gctcgacggc gagcgcggcg agcgcccggc cgaggcgccg
     1621 ttcgacctca tctcctcgag cctggccttc cagtggttcg agaatctgcc cgccgccctc
     1681 gcccggttgg cgggatggct ggctccgggc ggcacgctgg ccttcaccac ccttgccgag
     1741 ggcacctttg ccgaatggcg cgaggcccat gccgcgcttg gccttgcgag cggcacgccg
     1801 cactttccct cggcggagca gctcaggcgg atggctccgg ccgggttcca ggcggatatt
     1861 tcggtcgcca cccatgccga accgcagggc tcggccccgg ctttcctgaa ggcgctgaag
     1921 gcgatcggcg cggggacggc ggcggcggcg caccagcccc tgccgccggg cgccctgcgc
     1981 caggtcatgg cccggttcga cgcgggaggc gccgtggcca cctacgaagt cgccatctgc
     2041 ctgctgcgca gggaggccgc atgagcgcga tcgtgatcac cgg
//
LOCUS       DATNBV010000394         2887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1206975,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000394 DATNBV010000000
VERSION     DATNBV010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206975"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..1175)
                     /gene="prpB"
                     /locus_tag="VN627_09445"
     CDS             complement(282..1175)
                     /gene="prpB"
                     /locus_tag="VN627_09445"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HXD03519.1"
                     /translation="MSSSASPGSTFRAALAQERPLQIVGTINANHALLAQRAGYRAIY
                     LSGGGVSAGSLGMPDLGIIALEDVLTDVRRITQVCDVPLLVDIDTGFGPSAFNIARTV
                     KSLIRAGAAGCHIEDQAGAKRCGHRPGKEIVSTQEMADRVKAAADARTDSSFFLIART
                     DAIAVEGVAAAIERAQACIEAGADGVFAEAAYDLDTYRRFSGAVTAPLLANITEFGQT
                     PLFSIDELRSAGVAMALYPLSAFRAMNKAAEAVYTAIRRDGHQRNVIDQMQTREELYD
                     RIGYHAFEQHLDELFAKRDKP"
     gene            complement(1172..1945)
                     /locus_tag="VN627_09450"
     CDS             complement(1172..1945)
                     /locus_tag="VN627_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HXD03520.1"
                     /translation="MDGDAEVVVHGLRPGTYPAGVAPMDMNSIGALRMLNEQQVCEAA
                     LAAEAAGYDALALGCFFDPALHQARSLVDIPVVSLSESCMLTACSLGRRFAVVSLTDF
                     QKMLTEDLAASYGLSARLAGVVAMSPGVRLFDLEGSGAAAESIKERFAASCRRALELG
                     AEVVIPGDGVLNEFLVRHQMLSVDGAVVLDSLGVLFQHAAFLARGKARGTLGISRRLL
                     YARPTAAMLDHARGYYGALQREESAFTGGPVRAPEGGPR"
     gene            2213..>2887
                     /locus_tag="VN627_09455"
     CDS             2213..>2887
                     /locus_tag="VN627_09455"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXD03521.1"
                     /translation="MSATLPCTARRSLLRLATLAILAAPALLGAGPAAAQEFPSKPIR
                     LVVGYPPGGSNDIVARIIAPRLGEALNTTVVVENKAGVSGVIGADYVAKAAPDGYTLL
                     AASASPVVITPHTIAKIPFNTLTDFTPINTVGLTPEAIAVNPGLGVNSLKELLALSHT
                     RQVTLSSSGTGGLPHLTIELLIQASRGQIVHVPYKGAGPAVTDTLGGHVNGIVMDVPP
                     LYPMIQD"
BASE COUNT          462 a         1017 c          941 g          467 t
ORIGIN      
        1 cattgcgggc tcgacccgca atccaccgtg gcgcgccagc atggatcccc ccggatcagg
       61 tccggggcag gcttctccag tccgggatga cagccctcca gtccgggatg acagcccttt
      121 ttgtcattgc gggctcgacc cgcaatccac cgtggcgcca acatggatcc ccccggatca
      181 ggtccggggc aggcttctca agtccgggat gacagccctt tctgtcgcct cggatcaggt
      241 ccggggcagc aggctctgcc cgggatgaca acccgtaatt cctagggctt gtcgcgcttg
      301 gcgaacagct cgtccaggtg ctgctcgaac gcgtggtagc cgatgcggtc gtacagctcc
      361 tcgcgcgtct gcatctggtc gatcacattg cgctggtggc cgtcgcggcg gatcgccgtg
      421 tagaccgctt cggccgcctt gttcatggcg cggaatgccg acagcgggta cagcgccatc
      481 gccacgccgg cactgcgcaa ctcatcgatc gagaacaagg gcgtctggcc gaattcggtg
      541 atgttggcca gcagtggcgc cgtcaccgcg ccgctgaagc gccgataagt gtccaagtcg
      601 tacgcggcct cggcaaacac accgtcggca cccgcttcga tgcaggcttg cgcacgttcg
      661 atcgccgcag cgacgccctc caccgcgatc gcatcggtgc gcgcgatcag gaagaagctg
      721 ctgtcggtgc gggcgtccgc tgccgccttg acgcgatcgg ccatttcctg tgtggagacg
      781 atctccttgc ccggccggtg gccgcagcgc ttggcgccgg cctggtcctc gatgtggcag
      841 cccgcggcac cggcgcggat caggcttttc acggtgcggg cgatgttgaa ggcgctggga
      901 ccgaagccgg tgtcgatgtc caccaacagc ggcacgtcgc agacctgggt gatgcgccgc
      961 acgtccgtca gcacgtcctc cagggcaatg atgccgaggt cgggcatgcc cagcgaaccg
     1021 gcggagacgc caccgccgga gagatagatg gcgcggtagc cggcgcgctg cgccaacagc
     1081 gcgtgattgg cattgatggt tccgacgatc tgcagcggcc gctcctgcgc cagggctgcg
     1141 cgaaaggtac tgccggggct ggcgctcgag ctcatcgtgg gcctccttcg ggtgcccgga
     1201 ccgggccgcc ggtgaatgcg gactcctcgc gttgcagcgc gccgtagtag ccgcgcgcgt
     1261 gatcgagcat cgcggcggtg ggccgcgcat aaagcaggcg ccggctgatg ccgagcgtac
     1321 cgcgggcctt gccgcgcgcc aggaacgccg catgctggaa cagtacaccc agggaatcga
     1381 gcaccaccgc gccgtccaca gacagcatct ggtggcgcac caggaattcg ttgagcacgc
     1441 cgtcaccggg gatcaccact tcggcgccca gctccagtgc gcgccggcag ctggcggcaa
     1501 agcgttcctt gatcgactcg gccgcagcgc cgctgccttc gaggtcgaac agccgcacgc
     1561 ccggcgacat ggcgaccacg cccgccagcc gtgcgctcag cccataggac gccgccaggt
     1621 cctccgtcaa catcttctgg aagtcggtaa gcgacaccac cgcgaagcgg cgcccgagcg
     1681 agcaggcggt cagcatgcac gattcgctca ggctcaccac cggaatgtcg accagcgaac
     1741 gggcctggtg cagcgccggg tcgaaaaagc agccgagggc cagcgcgtcg tagcccgccg
     1801 cctccgccgc cagcgccgcc tcgcaaacct gctgctcatt gagcattcgc agggcgccga
     1861 tgctgttcat gtccatgggc gcgacgccgg ccggataggt gcccggtcgc agcccgtgca
     1921 cgaccacctc ggcgtcgcca tccatgattc gccggccatg ctcgcgcagc gtttcgagat
     1981 aaagcgggaa ttggtcgagg tccgtgaagc tctggtgcca gattcgcaat gggtttcctt
     2041 cgggcgcggg cccgcagcct gaatgggact gtgcccagca tggtaggccc gcaccgcctc
     2101 ggtttcgatt cggaatttac gaaaactgcc tttcccggct gcgactggcg cggcccggat
     2161 gcgctccgta gactttgcca tgcccctcac gcgcccgcaa aaggttgatt cgatgtccgc
     2221 gaccctcccc tgcaccgccc gccgcagcct gctgcggctg gcgacacttg ccatcctcgc
     2281 cgcaccggcc ctgttggggg ccggcccggc cgccgcccag gagttcccgt ccaagccgat
     2341 ccggctggtg gtgggctacc cgcccggcgg ctccaatgac atcgtcgcgc gcatcatcgc
     2401 gcccaggctg ggcgaggcgc tgaacacgac ggtcgtcgtg gagaacaagg ccggcgtcag
     2461 cggcgtcatc ggcgccgact acgtggccaa ggctgccccc gatggctaca cgctgctggc
     2521 cgccagcgcg agcccggtgg tgatcacgcc gcacacgatc gccaagatcc cgttcaacac
     2581 tttgaccgac ttcactccca tcaacaccgt gggcctgaca ccggaagcca tcgccgtgaa
     2641 cccgggcctg ggggtgaatt ccctcaagga actgctggcc ctgtcgcaca cccgccaggt
     2701 cacgctgtcc tcttccggca cgggcgggct gccccacctg acgatcgagt tgctgatcca
     2761 ggcctccagg ggccagatcg tgcacgtgcc ctacaagggt gccggcccgg ccgtgaccga
     2821 tacgcttggc ggccatgtca acggcatcgt catggatgtg ccgccgctgt acccgatgat
     2881 ccaggac
//
LOCUS       DATNBV010000395         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3431087,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000395 DATNBV010000000
VERSION     DATNBV010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3431087"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VN627_09460"
     CDS             complement(<1..851)
                     /locus_tag="VN627_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03522.1"
                     /translation="MTATTQDLDPYSADGICKGPEFFAELGRKCPVHHYQGRFDFFVL
                     SDPTDVRKSVLVDPEKWSIENGNGPSQLPERQRTGLISDDAYHFKIRNVIQRGFSSTE
                     LLRLKGIVENLANELIDAMEALPERRGDFFELFAMPLPARLMCLMLGAPEAEWKIYKA
                     WADQYFFNMYNDTQFDGEAGATGTTEITASLFAAMTERRALLEAKGLTPDLSLLGTVL
                     PNDFMSRFICEQIDGTPFSPAEVLSMMMGIIIGGNETTMNLIANLVWRLLEVPSRWEA
                     IKANPDL"
     gene            complement(907..1773)
                     /locus_tag="VN627_09465"
     CDS             complement(907..1773)
                     /locus_tag="VN627_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03523.1"
                     /translation="MSAEVGRLAGKAVLVTGAGRGIGFATALHLARSGAAIAVNDVDD
                     GLAREAANTIAGEGHRAIAVAGSVADWDRCAAMVDQTAQAFGRLDGLVNNAGLHYRAM
                     PWEEVPERVRATVEVNVLGAIFCAIHAARLMRGQPGGGAIVNITSGAAIGSQERAVYG
                     ATKGAMLSYTANLAGDLAPYGIRVNALAPFAATRMTDPACEEGDHGARSGEFADVPGP
                     QAMAPVIAWLLSDAAAEVTGSTIRFNGRQLSLIAPAALARPIIEQEQWSAAELDHALG
                     ALLRAEGQPSPR"
     gene            complement(1763..>2084)
                     /locus_tag="VN627_09470"
     CDS             complement(1763..>2084)
                     /locus_tag="VN627_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXD03524.1"
                     /translation="NADTCTAKHVWPGREVGEQTTQMKGFARTANPTSEIAAKVRERI
                     DVFYKVLQQEDYWVCGQAWENIRTGLVPELLYGRNEPALTWLHRSLDQAMEGFSRAET
                     ARVR"
BASE COUNT          364 a          691 c          648 g          381 t
ORIGIN      
        1 atcagatcgg ggttggcctt gatcgcctcc cagcgcgacg gcacctccag caggcgccag
       61 accaggttgg cgatcaggtt catggtggtc tcgttgccac cgatgatgat ccccatcatc
      121 atcgacagca cctcggcagg cgagaacggg gttccgtcga tctgctcgca gatgaagcgc
      181 gacatgaaat cgttcggcag cacggtgccg aggagcgata ggtccggggt caaccctttc
      241 gcctcgagca gcgcccggcg ctcggtcatg gccgcaaaca gtgaggcggt gatctcggtg
      301 gtgccggtgg cccctgcttc accgtcgaac tgggtgtcgt tatacatgtt gaaaaaatac
      361 tggtcggccc aggccttgta gattttccac tcggcttccg gcgcacccag catcaggcac
      421 atgagtctgg cagggagcgg catggcgaac agctcgaaga agtcgccccg gcgctcgggc
      481 agcgcctcca tggcgtcgat cagttcgttg gcgaggttct cgacgatccc cttcagccgc
      541 agcagttcgg tggaggagaa cccgcgctgg atcacgttgc ggatcttgaa gtgataggcg
      601 tcatcgctga tcaacccggt gcgctgccgc tccgggagct ggctgggccc gttgccgttc
      661 tcgatgctcc acttctcggg atcgaccagc acggacttgc gcacgtcggt cggatcgctg
      721 agcacgaaaa aatcgaaccg accctggtaa tggtgcaccg ggcattttcg gccaagctcg
      781 gcaaagaact ccgggccctt gcagatgcca tcggcagaat aggggtcgag gtcttgagtc
      841 gttgcggtca ttgcgcaaat cccgatctgg tgggtaaacg tgcgactggc cggccggcca
      901 ttagccttat cttggtgaag gctggccttc ggcgcgcagc agggccccaa gcgcatggtc
      961 cagctccgcc gccgaccact gctcctgctc gatgatcggc cgcgccagcg cagccggggc
     1021 gatcagcgac aactgccggc cgttgaagcg tatggtactc cccgttacct ccgccgccgc
     1081 atcggacaac agccaggcaa ttaccggcgc catcgcctgc gggccaggaa cgtccgcaaa
     1141 ctcgcccgac cgtgcgccat gatccccctc ctcgcaggcc ggatcggtca tgcgggtggc
     1201 agcaaagggg gcgagcgcat tgacgcggat gccgtacggc gccaggtctc ccgcgaggtt
     1261 ggccgtatag gaaagcatcg cccccttggt agcgccgtag acagcgcgtt cctgggagcc
     1321 gatcgctgcg cccgatgtga tattaacgat ggccccgcca cctggttgcc cgcgcatcag
     1381 ccgcgccgca tggattgcgc agaaaatggc gcccaggacg ttgacctcaa cggtcgccct
     1441 gacgcgttcc ggcacctctt cccagggcat ggcccggtaa tgaagccctg cattgttgac
     1501 gagcccgtcg agcctgccga acgcttgggc agtctggtcg accatcgccg cgcaccgatc
     1561 ccagtcggcc acgctgcccg ccacggcgat ggcccggtgc ccttcaccgg caatcgtgtt
     1621 ggcggcttca cgagcgagcc cgtcatcgac atcgttcacc gcgatcgcag ccccgcttcg
     1681 cgccaggtgc agggccgttg caaacccgat gccgcggccg gcgccggtta caagcaccgc
     1741 cttgccggcg agtctcccca cctcagcgga cacgggccgt ctccgctctg gaaaatccct
     1801 ccatggcctg gtcaagcgac cggtgcagcc aggtcagggc aggctcgttg cggccataga
     1861 gcaattcggg aacaaggccg gttctgatat tttcccaagc ctggccacag acccagtaat
     1921 cttcctgctg cagaaccttg tagaagacat cgatccgctc acggaccttt gctgcaatct
     1981 cagaggtggg attggcagtt cgcgcgaacc ccttcatctg cgtcgtctgc tcgccgacct
     2041 cccggcccgg ccagacgtgc tttgcggtgc aagtgtcggc gttc
//
LOCUS       DATNBV010000396         3930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_864594,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000396 DATNBV010000000
VERSION     DATNBV010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864594"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VN627_09475"
     CDS             <1..233
                     /locus_tag="VN627_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXD03525.1"
                     /translation="GSFPPSTGPTPEAGERTLSNIVQIHEARAIMAALSETGGNRLAA
                     ARKLGISERTLRYRLAAFREAGLAVAAAGGRR"
     gene            230..607
                     /gene="fliE"
                     /locus_tag="VN627_09480"
     CDS             230..607
                     /gene="fliE"
                     /locus_tag="VN627_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HXD03526.1"
                     /translation="MSGVGPIGGGGGGIQQILAMRQQIIDRSNLLQQVRGASPATEAA
                     PNGSVGAPAEGFSGALKQALAGVSASQQKAEDMSSAYEKGEVTDIAKVMLARQEAGVA
                     FEATLQVRNRLLSAYQDIMRMGV"
     gene            611..2260
                     /gene="fliF"
                     /locus_tag="VN627_09485"
     CDS             611..2260
                     /gene="fliF"
                     /locus_tag="VN627_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HXD03527.1"
                     /translation="MAELVPAAEGAALAPPSLLAPLTDPAGGPVLTRLGAFTRQPAVR
                     KTLPWFGGMAGIGMAALAWATLAPQAQRTLYSELDDGERAKVTASLDKAGIGYHIDNQ
                     TGALTVGEDDLYKARMLVASDGALASPDEGAASLDKLPLGASRAVETDRLRAAREHDL
                     TLSIMEIDGIEAVRVHLAEAEKSVFVRDNVAPSASVMVRLARGRQLGDSQTAAIVNLV
                     AGSVPGLSPDAVRVVDQHGRLLTEKASGNSERLELQGQLEDKLRAQLDQLLTPMLGQG
                     NFSSEVQVELDMDQVTSARESYDKQGVLRSETQAQSQTTGTGGAAGVPGVTANTPPPA
                     PTASPGPPQGSAPAPAAAAQNNGESSATRTYELGREVAVSNGTPGKIKRLTVAVALSA
                     DALKKTKPQDIEQLKQLIGAAVGVDPARGDQVAVVLRAFDAAPVDSLPLWEQPWFQTY
                     VRYGVALLGVLLVLLLGVRPLIKALKRSPAAAADADSDDDPASAEDAEGRPRPALKAG
                     VDPAELGQQLGLAQRIAAERPDDAVLALREMLNHAPQEEAA"
     gene            2257..3282
                     /gene="fliG"
                     /locus_tag="VN627_09490"
     CDS             2257..3282
                     /gene="fliG"
                     /locus_tag="VN627_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HXD03528.1"
                     /translation="MTAPMMLDPAALSEADRAAVMVMLLEEDQAAQILARLEPGELRL
                     LGEKMCALGEIGPEVIAHAIAGFVEKTERLGLKAHDRLGQVRSLMTRAVGDVRAGNLM
                     QRILPPDHKPPSPLELAKWLNPEALVPLIREEHPQAVAVLLVQLDPEVAAEVLHALPP
                     ELQPQVVHRIATLGPVAPEALAMLEDLLRSRIESVHGGVPLAMGGPREAAEIINSAGK
                     VVEKRVIPEITKLDKALAKEIESEMFKFEHLFVLDAQAMGALLREVESDSLIDALKGI
                     DEEQRNVFFRAMSSRAADGIKDEIAGRGRLKMADVVAAQKAIVAVARRLAAEGTIVFG
                     SGDDDYV"
     gene            3470..>3930
                     /locus_tag="VN627_09495"
     CDS             3470..>3930
                     /locus_tag="VN627_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HXD03529.1"
                     /translation="MAAAQAEARAALDLSLIPGALDRAFQQASGGPGGGLTLSLQLLV
                     IMGLLTVLPSLVLMMTSFTRILVVLGILRQALGLQQSPPNQVLVGLSLFLSLFVMAPT
                     LDKVSAAAIQPYASGQINAEQAIGNAGQQFHAFMIRQTRQHDLAMFADMAKA"
BASE COUNT          633 a         1413 c         1340 g          544 t
ORIGIN      
        1 atggctcctt ccccccgagc accggcccca ccccggaggc cggcgagcgc accctctcga
       61 atatcgtcca gatccatgaa gcccgcgcca tcatggccgc gctcagcgag acgggcggca
      121 accgccttgc cgcagcccgc aagctgggta tctcggagcg caccctgcgc tatcgcctcg
      181 ccgccttccg cgaggccggc cttgccgtcg ccgcggcggg cggtcggcga tgagcggcgt
      241 cggcccgatc ggcggcggtg gcggcgggat ccagcagatc ctggccatgc gccagcagat
      301 catcgaccgc tccaacttgc tccagcaggt ccgcggcgcg tcacctgcca ccgaggctgc
      361 acccaatggt tcggttgggg ccccggccga aggcttctcc ggcgcactca agcaggcgct
      421 cgctggggtc agcgccagcc agcaaaaggc cgaggacatg tcgagcgcct atgaaaaggg
      481 cgaggttacc gacatcgcca aggtcatgct ggcccggcag gaagcgggcg ttgccttcga
      541 ggcgacgctg caggtgagga accgcctgct ttcggcctac caggacatca tgcggatggg
      601 agtctgatag atggccgagc ttgtccctgc cgccgagggc gccgcccttg ccccgccctc
      661 gctccttgcc ccgctcaccg atccggcggg cgggcccgtg ctcacccggc tcggcgcctt
      721 cacccgccag ccggccgtgc gcaagaccct gccgtggttc ggcggcatgg cagggatcgg
      781 catggccgcg ctcgcctggg caaccctcgc cccgcaggcc cagcgcacgc tctattccga
      841 actcgacgac ggcgaacgcg ccaaggtgac cgcgagcctc gacaaggccg ggatcggcta
      901 ccacatcgac aaccagaccg gcgccctcac cgtgggcgag gacgatctct acaaggcccg
      961 catgctcgtc gcttccgacg gggcactggc ttcgcccgac gaaggcgcgg caagcctcga
     1021 caagctgccg ctgggcgcga gccgcgcggt cgagaccgac cgcctgcgcg ccgcgcgcga
     1081 gcatgacctg acgctctcga tcatggaaat cgacgggatc gaggcggtcc gcgtgcacct
     1141 tgccgaggct gagaagtcgg tcttcgtgcg cgacaacgtg gcccccagcg cctcggtcat
     1201 ggtccgcctc gcccgcggcc ggcagctggg cgacagccag actgcggcca tagtcaatct
     1261 cgtcgccggc tcggtccccg ggctcagccc cgatgcggtg cgggtagtcg atcagcatgg
     1321 gcgcctgctt accgagaagg ccagcggcaa ttcggagcgg ctcgaactgc agggccagct
     1381 ggaagacaag ctgcgcgcgc agctggacca gttgcttacc cccatgctcg gccagggcaa
     1441 cttctcaagt gaggtccagg tcgagctcga catggaccag gtaaccagcg cccgcgagag
     1501 ctatgacaag caaggcgtgc tgcgcagcga gacgcaggcg cagtcgcaga ccactggcac
     1561 cggcggcgca gccggcgtgc ccggtgtcac ggccaacacg ccgccgccgg cgcccaccgc
     1621 ctcgcccggc ccgccgcagg gctcggcgcc ggcaccggct gcggcggcac agaacaacgg
     1681 cgaatccagc gcgacccgga cctatgagct gggccgcgag gttgccgtgt ccaacggcac
     1741 gccgggcaag atcaagcggc tgaccgtcgc ggttgcgctg agcgccgatg cgctcaagaa
     1801 gaccaagccg caggacatcg agcagctcaa gcagctgatc ggcgcggcgg tgggcgtcga
     1861 tcccgcgcgc ggcgatcagg ttgccgtggt gctgcgcgcg ttcgacgcgg ccccggtgga
     1921 cagcctgccc ctgtgggagc agccgtggtt ccagacctat gtgcgctacg gcgtggccct
     1981 gctgggcgtg ctgctggtgc tgctgctggg cgtgcgtccg ctgataaagg cgctgaagcg
     2041 cagtcccgcc gctgctgccg acgcggacag cgacgacgat ccggcgagcg ccgaggacgc
     2101 cgaagggcgc cctcgcccgg cgctcaaggc cggggtcgat ccagcagaac ttggccagca
     2161 gctcggcctt gcccagcgca tcgcggcgga gcggcccgac gatgccgtgc tcgccctgcg
     2221 cgagatgctc aaccacgcgc cgcaggaaga agccgcatga ccgctccgat gatgctcgat
     2281 cccgcagcgc tgagcgaggc cgaccgggcc gcggtcatgg tcatgctgct cgaggaagac
     2341 caggccgcgc agatccttgc ccggctggag cccggcgagc tgcggctgct gggcgagaag
     2401 atgtgcgccc tgggggagat cggccccgaa gtgatcgccc atgccatcgc ggggtttgtc
     2461 gagaagaccg agcgcctcgg cctcaaggcg cacgaccgcc tgggccaggt ccgcagcctg
     2521 atgacccgcg cggtgggcga cgtgagggcc ggcaacctga tgcagcgcat cctcccgccc
     2581 gaccacaagc cgccaagccc gctcgaactg gccaaatggc tcaatcccga ggcgctggtg
     2641 ccgctgatcc gcgaggaaca cccccaggct gtcgccgtcc tgctggtcca gctcgatccc
     2701 gaagttgccg ccgaagtgct ccatgcgctg ccgccggaac tccagccgca agtggtccac
     2761 cgcatcgcca cgctcggccc ggtcgcgccc gaggcgctgg ccatgctcga ggacctgctg
     2821 cgcagccgca tcgaaagcgt tcacggcggc gtaccgctgg ccatgggcgg cccgcgcgag
     2881 gcggccgaga tcatcaacag cgccggcaag gtggtggaaa agcgggtgat ccccgagatc
     2941 accaaactgg acaaggcgct cgccaaggaa atcgaaagcg agatgttcaa gttcgagcac
     3001 ctgttcgtgc tcgatgccca ggcgatgggc gccctgctgc gcgaggtgga gagcgacagc
     3061 ctgatcgatg cgctcaaggg catcgacgag gagcagcgca acgtcttctt ccgcgccatg
     3121 tcgagccgcg ccgccgatgg catcaaggac gagatcgccg gtcgcggccg cctcaagatg
     3181 gccgacgtgg tcgcggcgca gaaggcgatc gtcgccgtcg cccggcgcct cgccgccgag
     3241 ggcacgatcg tgttcggctc gggcgacgac gactatgtct agtctcgccg cgcttaccgg
     3301 cggaccgccc gataccggcg gatttcgcag cgacatgcgc ttcgccctgc ccgtccgggc
     3361 tgcccggccg ccggagccgg agctggtgca gatcgaggac ccggtcgccc tcgcctgggc
     3421 cgaaggttat gcccgcgggc tggacgatgc ccaggccgca gcaaccgaaa tggccgccgc
     3481 gcaagccgag gcccgcgccg cgctggacct gtcgcttatc cccggggcac tcgaccgggc
     3541 gttccagcaa gccagcggcg ggcccggcgg cgggctgacg ctgtcgctcc agcttctcgt
     3601 catcatgggg ctgctgacag tcctgcccag ccttgtcctg atgatgacga gcttcacccg
     3661 catcctcgtg gtcctaggca tcctgcgtca ggcgctgggc ctacagcaga gcccgccgaa
     3721 ccaggtgctg gtcggcctct cgctgtttct ctcgctgttc gtcatggcgc cgacgctgga
     3781 caaggtcagc gccgcggcga tccagcccta tgcgagcggg cagatcaacg ccgagcaggc
     3841 gatcggcaat gccggacagc agttccacgc gttcatgatc cgccagaccc gccagcacga
     3901 tcttgccatg ttcgccgaca tggccaaggc
//
LOCUS       DATNBV010000397         3390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1810891,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000397 DATNBV010000000
VERSION     DATNBV010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810891"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VN627_09500"
     CDS             <1..243
                     /locus_tag="VN627_09500"
                     /inference="COORDINATES: protein motif:HMM:NF023965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-hydroxybutyrate polymerase N-terminal
                     domain-containing protein"
                     /protein_id="HXD03530.1"
                     /translation="GETAALRPLPASPQTDLGLAAGHHAYSSVDRAFKANLARLTLGL
                     SPAVLAEQTFDWLTDLEVDPENGTGALIGTGPFPSW"
     gene            253..1349
                     /locus_tag="VN627_09505"
                     /pseudo
     CDS             253..1349
                     /locus_tag="VN627_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611153.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1915..3078)
                     /locus_tag="VN627_09510"
     CDS             complement(1915..3078)
                     /locus_tag="VN627_09510"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXD03531.1"
                     /translation="MAQQLDDLSRSPTPLDQDSTIITVIEMSQATWLVAGIVPGIERH
                     PLKKIEPNEVELLCLLMRWRAEAMKKGRTITRIVVAFEAGRDGFWLARWLRVRDIESY
                     VIHPTSVAVSREHRRAKTDRLDTGLLKRVFIGWLRGEPDHCHMAAIPTLAEEDAKRPN
                     REREGLVRERTRLVNRMKSCLVRFGVRTFKPTLRNAPDRLATLHTPEGVPLPPNTLLE
                     LRRDMARLRFVMDQIKEVEAARAQGLKQAPQEKTHAMVNLLARILGIGIDTADMLVHE
                     VLARNLRDRRAVARYAGLTGSPDESGSRRREKGLAKAGSARVRRGMIQLAWRFLLFQK
                     ESALAQWYRARTADARGGTRKTMIVALARKLLIALWRMLTTGEVPAGVVLRAA"
BASE COUNT          589 a         1057 c         1142 g          602 t
ORIGIN      
        1 ggcgaaacgg ccgcgcttcg tcctttaccc gcctcgccgc aaacggactt gggacttgca
       61 gcaggccatc atgcctattc gagtgtcgat cgagcattca aggcgaatct ggcgaggctc
      121 acgcttggcc tgagtccagc cgtgctcgcc gagcagacat tcgactggct cacggaccta
      181 gaagtggacc ccgaaaatgg gaccggggcg ctaattggaa cgggtccgtt tccgtcgtgg
      241 tagacggaga cgatgatgag gaagccgaga cggacgatcg atgcggcgct gaaggcgaag
      301 attgcgcttg aggcgctgcg ggaacaggcc acggtgacgg agctggcggt gcgccatgag
      361 gtgcacccga accagatcta cgcctggaag aagcagctgc tcgacaacgc cgcgcgggtg
      421 ttcgatgcca aggtcggggt cgaggcggag gagcgcgccg ccagggagaa ggcggacctc
      481 tacgccaaga tcggccagct caccgtggaa cgggattttt tatcgcggag gtccggacga
      541 tgagcacgcc ggaccgcaga gcgctgctcg agcgcgccca cccggaattg tcggtgcgcc
      601 ggcaatgcac cctgctgagc ctggcccgct ccggggtgta ccggcccatc ccggtcgagg
      661 acgaaacgga cctggggctg atgcgccgga tcgacgagct gttcctggcg cacccgttcc
      721 tgggctcgcg ccggatggcg cggatgctgt cgcgggatgg cgcgccggtg aaccgcaagc
      781 gggtgcagcg cctgatgcgg ctgatgggga tcgaggcgct cggtccgaag ccgaacacct
      841 cgcagccggc gccggggcac aagatctacc cctacctgct gcggggcttg acgatcgagc
      901 ggcccaacca cgtctgggcg gcggacatca cgtatatccc gatcgggcaa ggcttcctgt
      961 acctggtggc gatcatcgac tgggcgagcc gttcagtgtt gtcgtggcgg ctgtcgaaca
     1021 ccatggacac gagcttctgc atcgatgcct tggaagacgc gctcgctcgg ttcggacggc
     1081 cggaaatctt caacactgac cagggttcgc agtttacaag ctcgtccttt actggtaagc
     1141 tgttggccgc tgggatcagg atctcgatgg acggacgcgg tcgctggctg gacaacgtgt
     1201 tcatcgagcg gctgtggcgt tcgctcaagt acgaggacgt gtatttgaag ggctatgcgg
     1261 acggccgcga ggcgcgggcc gggattgcgg cctggatcgc cttctacaac gcccggcggc
     1321 cgcatcaggc gctgggcggg caggctccga tggtggtctg gcgtgccggc acgggcggcg
     1381 ccctcggcgc caaggctgtg gacctgacgc ttcgcttgga caacgctgac gcgttgccca
     1441 caggcccaca gcagcagcag gcccttgtat ggggcatctg gtgtcaagcg gtttcagtcg
     1501 acatggcttt ggacctgacc aaggttgcgc tggccggggc atggtcgccc cctttcggcc
     1561 cgcgtcgcgc cggccgagca tcgacgttgt cgctacaagc ggcccctgac ctggggaacc
     1621 cgagggtaag tcgcgcccgc ttttctcaaa ccgatcgggt gcattgacga tcagagggga
     1681 gcttgccatc gggcgcggat catcgcgcca caaccttgta ttcggtaggt cccgggcggg
     1741 ccctgaccat gatgcagtca tgcgcatcgg cggcgaagct ccggggcggc gggcccattc
     1801 tcgaaaacgg tatcgacacc atggcctgct tcgggtgacc gccacctcgg atcgtcagcg
     1861 gagaccgacg atatcctgtt cgtcagggtt ccgcttgtcc ctgtctgcgc ctcctcacgc
     1921 ggctcgtaag acgacgccag ccggcacctc gccagtggtc agcattcgcc acagcgcgat
     1981 tagcagcttt ctggccaggg ccacgatcat ggtcttgcgc gtgccgccac gggcgtcggc
     2041 agtccgcgcc cggtaccact gcgcgagcgc actctccttc tggaacagca gaaagcgcca
     2101 agccagttgg atcatcccgc gccggacgcg ggcgctgccc gccttggcaa gccctttctc
     2161 gcgccgccgg ctgccgctct cgtctggtga gcctgtcagg cccgcatagc gggcgacggc
     2221 tcttcgatcg cgcaagttcc gtgcaaggac ttcgtggacc aacatgtccg ccgtatcgat
     2281 gccgatgccc aaaatgcggg cgagaagatt gaccatggcg tgcgtttttt cctgaggagc
     2341 ctgcttcagc ccttgcgcac gggccgcctc gacctccttg atctggtcca tgacgaaccg
     2401 cagtctggcc atgtctcgac gaagttcgag cagcgtgttg ggcgggagag gcaccccctc
     2461 gggcgtatgc agcgttgcga gacggtccgg cgcgttgcgc aacgtcggct tgaaggtccg
     2521 caccccaaag cggaccagac aactcttcat ccggttgaca aggcgcgtcc gctcacggac
     2581 gagtccctcg cgttcccggt tcgggcgctt ggcatcctcc tcagccagcg tcgggatcgc
     2641 cgccatgtgg cagtgatcgg gttcaccgcg cagccacccg atgaagacgc gtttgagcaa
     2701 tcccgtgtcg agccgatctg tttttgcccg gcgatgctcg cgcgagacag ccacgctggt
     2761 cgggtggatg acgtagcttt cgatgtcgcg gacccgaagc cagcgcgcca gccagaaccc
     2821 gtcgcgccca gcctcgaagg caaccacaat gcgcgtgatc gtacgacctt tcttcatggc
     2881 ctctgcacgc cagcgcatga gtaaacagag cagctctacc tcgttcggtt caatcttctt
     2941 cagcgggtgg cgctcgatac ctggaacaat gccggcgacg agccaggtcg cctgactcat
     3001 ctcgatgacc gtaatgatcg tgctgtcttg gtccaaggga gtgggggacc tgctcagatc
     3061 gtcgagttgc tgtgccatga gagcgctcca tcgcttccag cgacgatggt gacatggcgc
     3121 caccgtcggc cccatagcat ctcgaaagtg aagatgcgga tgccgacccg cctgagatac
     3181 ggggaaggct gcgtgagcgg ggaagcaatc gacacaagca cccgtctcga tccgccgggg
     3241 tagtgggcac ggcacgccgg aacggtgaag cgggtaacgg gggagaccca tctggggcgg
     3301 gggtagcggc ctcaacgctg cttctggcag cggcacaggt gggagtcgga caggggcata
     3361 gtactgttga agccgggcaa tgccggtgga
//
LOCUS       DATNBV010000398         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3056167,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000398 DATNBV010000000
VERSION     DATNBV010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3056167"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1279
                     /locus_tag="VN627_09515"
     CDS             53..1279
                     /locus_tag="VN627_09515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HXD03532.1"
                     /translation="MDIPGLPARRAALKLLDAVLRRGETLDQAGHNAVQGLAADADKA
                     LARAIAAEALRWLPDLDALIDSATRQPLPDDAKPRAVLRLMLAQWLRLGTPPHAVVAT
                     ALPLLAGGPKRLAHGVFATLTRQGAALPPQPSLPPAVAARWGERAPAIAAALAEPPLL
                     DLTLKDPATTAEWAERLGATSLMAGHLRLPRGEAIERLPGFADGAWWVQDLAASLPAR
                     LLGSGPGRALDLCAAPGGKAMQLSAAGWDVVALDASAKRLERLSANFARVGLPVEVVV
                     ADALDWAGEERFDAILLDAPCTATGTCRRHPDVLHRITGTQIREMAELQQRLLSRAAG
                     WLKPGGRLVYAVCSLEQEEGEAIAAEIPLERLPIRAEELPAGLAPTAEGWLRTDPGML
                     AAAGGLDGFFVARWSA"
     gene            complement(1295..1723)
                     /locus_tag="VN627_09520"
     CDS             complement(1295..1723)
                     /locus_tag="VN627_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD03533.1"
                     /translation="MANDPTDELQIRNIIARLAHAADMAGEDQLDTAYVPLFTEDAVW
                     EALPGALGPSAIRNEGIAAIRAAAVDRRRAGTSGPAAGSMHLVTTTALAIAGDEASGL
                     SCFMVIGGADRAITAAARYEDRFRRVDGQWRLAHRTVRPR"
     gene            complement(1819..>2180)
                     /locus_tag="VN627_09525"
     CDS             complement(1819..>2180)
                     /locus_tag="VN627_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXD03534.1"
                     /translation="QYAQDNGLGTRAIHTLSAISEGHVRPGELARLMLLPPSVVSGDL
                     NQLRDAGLIRRDRDPRDGRRLVYGLTAPGETMLAGAHRLFVQLLGEKLRSHPEAEVAS
                     TLRLLYNLTRQLRESMG"
BASE COUNT          279 a          770 c          807 g          324 t
ORIGIN      
        1 cggtgacatg ggcgctctgg ccagccaatg caatcgcatc taggggcacc ccatggatat
       61 tcccggcctt cccgcccgcc gcgccgcgct caagctgctc gatgccgttc tgcgccgggg
      121 cgaaacgctc gaccaggcgg ggcacaacgc ggtgcagggc ctcgccgccg atgccgacaa
      181 ggcgctggcc cgcgccatcg ctgccgaagc cctgcgctgg ctgcccgatc tcgatgccct
      241 gattgacagc gccacccgcc agccgctgcc cgatgatgcc aagccgcgcg ccgtgctgcg
      301 gctgatgctg gcccagtggc tgcggctggg gacgccgccg cacgcggtgg tcgccacggc
      361 cctgcccctg ctggccggcg ggcccaagcg gctggcgcat ggggttttcg ccacgctgac
      421 ccggcagggc gccgccctgc cgccgcagcc gagcctgccg ccggccgtgg cggcgcgctg
      481 gggcgagcgg gcgccggcca tcgctgccgc gctggccgaa ccgccgctgc tcgacctgac
      541 gctgaaggac ccggcgacga ccgccgagtg ggccgaacgg cttggcgcca cctcgctgat
      601 ggcggggcac ctgcgcctgc cgcgcgggga ggcgatcgag cgcctgcccg gttttgcgga
      661 cggggcctgg tgggtgcagg accttgccgc gagcctgccc gcgcgcctgc ttgggtccgg
      721 gcccggccgg gcgcttgacc tgtgcgcggc cccgggcggc aaggccatgc agctctccgc
      781 cgccgggtgg gacgtggtgg cgcttgatgc cagtgccaag cggctggagc ggctgtcggc
      841 caactttgcc cgcgtcggcc tgccggtgga ggtggttgtc gccgatgcgc tcgactgggc
      901 aggcgaggag cggttcgatg cgatcctgct cgacgcgcca tgcaccgcga ctggcacctg
      961 ccgccggcac ccggacgtgc tgcaccggat caccggcacc cagatccgcg aaatggccga
     1021 gctgcagcag cggctgctga gccgggcggc agggtggctc aagcccggcg gtcggctggt
     1081 ctacgcggtg tgctcgctgg agcaggaaga gggcgaggcg attgctgccg agattccgct
     1141 ggagcggctg ccgatccgcg cggaggagct gcccgccggg ctcgcgccga cggcggaagg
     1201 ctggctgcgc accgatcccg gcatgctggc cgccgccggc gggctggacg ggttcttcgt
     1261 tgcgcggtgg agtgcctgag cgggccccgt gtgttcagcg cggccggacg gtgcggtggg
     1321 cgagacgcca ctggccatcc acgcgccgga accggtcctc atagcgggcc gcggcggtga
     1381 tggcccgatc ggccccgcca atcaccatga agcaggacag gccgctggcc tcgtccccgg
     1441 cgatggccag ggccgtggtg gtgacgagat gcatcgatcc ggctgccggc ccgctggttc
     1501 ccgcccgccg ccgatcgacg gcggcagcac ggatcgcggc gatgccttcg ttgcgaatgg
     1561 ccgacgggcc cagcgcgccg ggcagtgctt cccacacggc atcttcggtg aacagcggca
     1621 cataggccgt atcgagctgg tcctctccag ccatgtcggc cgcgtgggcc aggcgggcaa
     1681 tgatattgcg gatctgcagt tcgtcggtcg ggtcgttggc catcatcggc tcctctcggg
     1741 ggcgagaggt agacctcgtg gggccggccc ggcaagcgat ggctgcgacc gctcatgcgg
     1801 cgggcacatc cgcgatgctc agcccatgct ctcgcgcagt tgcctggtca ggttgtaaag
     1861 cagccgcaag gtgctggcga cttcggcttc cgggtggctg cgcagcttct ctcccagtaa
     1921 ttgcacgaag agccggtgcg cccctgccag catggtctcg cccggcgccg tcagcccata
     1981 gacgagacgt cgcccgtcac gcggatcgcg gtcgcgccgg atcagaccgg catcgcgcaa
     2041 ctggttgaga tcgccggaga ccacgcttgg cggcaggagc atcagccggg caagttcgcc
     2101 gggcctcacg tggccctccg atatcgcact cagcgtgtga atggcacggg tgcccagccc
     2161 attgtcctgc gcatattgcg
//
LOCUS       DATNBV010000399         3552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_537577,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000399 DATNBV010000000
VERSION     DATNBV010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537577"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VN627_09530"
     CDS             <1..491
                     /locus_tag="VN627_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXD03535.1"
                     /translation="EAEKHDVPTEPVDLVSLVRQVAGEATALAGKDRVALSLPASPLA
                     MPGDRAQLEQLVRNLIDNALKYGGVEEPVDVGLSGGDDGIVLTVRDHGPGIAPEHLPH
                     LTRRFYRADPGRSRASGGTGLGLAIVKHIVERHRGRLDIASTLGQGTTVTAVFRPVGR
                     VS"
     gene            551..1594
                     /locus_tag="VN627_09535"
     CDS             551..1594
                     /locus_tag="VN627_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXD03536.1"
                     /translation="MTRTSFKLAAASALCALLAACGSGQNATRDQVRAVGSSTVYPFA
                     KIVAANFAKDNAGFKAPIVETNGTGGGLKLFCAGVGPQHPDVADASRRIKKAEFEECQ
                     KNGVTDISEIQVGIDGIAFATGTTGPVLALTPVDVYKAIAANPFGKPNTAKTWKDVNP
                     AFPATPIQVYGPATISGTRDALKELILVKGCETDAATKALKDSNKEKFDAICGDLRTD
                     GAYADTSDNYNLIVQKIGTNPQAVGIFGYSYLESNLDKLKALTMGGVSPTYETISNFT
                     YPGARPLFIYVKKAHLEAIPGLKEFVAEWAKSWGKDGPLAKQGLIVMPADKLAASAKA
                     ASEFPSLNGADLK"
     gene            1622..3019
                     /gene="pstC"
                     /locus_tag="VN627_09540"
     CDS             1622..3019
                     /gene="pstC"
                     /locus_tag="VN627_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HXD03537.1"
                     /translation="MSPVAPVLLVLGLGLIGWLVTRARARGFAAGPGATSGSARPHSL
                     AQYHAWYVALWTVVPALLFALVWASFSPGLALHQALSSPAAAQLPAFGFQRDRIIAEA
                     QAVAMGQATAVFNPAARALVEPFRAALGRFGLIGAGITLLLAAAGGGYAFTRIKAQFA
                     ARTSVERAVMVLLFAASLVAIFTTFGIFFSLLFETIRFFRIVSPVDFLFGMHWQPDPM
                     AAISPTTGENYGAVALFWATIFIGAVIAMVVAIPIGLMSAVFLTQYASPGLRKVMKPL
                     LEILAGVPTVVYGYFAALIVAPAVRDFGHAIGIASASSESAAAAGLVMGIMIIPFVSS
                     MADDAIAAVPQSLRDGSLAMGATTAETIRKVLIPAALPGIVAGIMLAVSRAIGETMIV
                     VMAAGATARLSANPFESMTTVTYQIVAMLTGEGSFDHPATLAAFALGMVLFVVTLSLN
                     FIALSVVKRYREAYD"
     gene            3012..>3552
                     /locus_tag="VN627_09545"
     CDS             3012..>3552
                     /locus_tag="VN627_09545"
                     /inference="COORDINATES: protein motif:HMM:NF023240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3333 domain-containing protein"
                     /protein_id="HXD03538.1"
                     /translation="MTELTPLASADKQARLAARIKQRHRSDRLFRLAGMGAVAFAGFV
                     LAFLLFTMTANGLGGFQRAELKVPVNLANGVIQLDPSQLASGNPVAALESAGLPALVD
                     SELERAYGKDGAQLVVDGAWRELARRIVKDPALLTHQVDAWLPASNDLAAALRGEGTP
                     AELAQGRGLAQQGVLAEHFD"
BASE COUNT          558 a         1281 c         1122 g          591 t
ORIGIN      
        1 tcgaagcgga aaagcacgac gtgccgaccg agccggtcga cctcgtctcc ctggtccgcc
       61 aggtggccgg cgaagcgacg gcgctcgccg gcaaggaccg ggttgcacta tccctgccgg
      121 ccagcccgct ggcgatgccc ggtgaccggg cccagctcga gcagctcgtg cgcaacctga
      181 tcgacaatgc gctgaaatat gggggcgtgg aggagccggt cgacgttggc ctcagcggtg
      241 gggacgacgg catcgtgctg accgtgcgcg accatggccc cggcatcgcg ccggagcacc
      301 tgccgcacct cacccggcgg ttctaccggg ccgatccggg ccgcagccgc gcctcgggcg
      361 gcacgggact ggggctcgcc atcgtcaaac acatcgtcga gcgccaccgc gggcgactcg
      421 acatcgccag caccctcggc cagggaacca cggtaacggc ggttttccgt cctgtcgggc
      481 gcgtgtcatg aaagtgtcat cgaactccaa cagaggcagg ccaacgcaat ccagaatgga
      541 gcctgccgtg atgacccgta cctcgttcaa gcttgccgcc gccagcgccc tgtgcgccct
      601 cctcgccgcc tgcggctccg gccagaatgc cacgcgcgac caggtccgcg cggtcggctc
      661 gtccaccgtc tatccgttcg ccaagatcgt tgcggcgaac ttcgccaagg acaatgccgg
      721 gttcaaggcg ccgatcgtcg aaaccaacgg cacgggcggc gggctgaagc tgttctgcgc
      781 cggcgtcggc ccgcagcatc ctgacgtcgc cgacgcctcg cgccgcatca agaaggccga
      841 gttcgaggaa tgccagaaga acggcgtgac cgacatttcg gaaatccagg tcggcattga
      901 cggcatcgcc tttgccacgg gcaccaccgg cccggttctc gccctcaccc cggtcgacgt
      961 gtacaaggcg attgccgcca accccttcgg caagcccaat acggccaaga cctggaagga
     1021 cgtgaacccc gcgttcccgg cgactccgat ccaggtctac gggccggcca ccatctcggg
     1081 cacccgcgac gcgctcaagg aactgatcct cgtcaagggc tgcgagaccg acgccgccac
     1141 caaggcgctc aaggacagca acaaggaaaa gttcgacgcg atctgcggcg acctgcgcac
     1201 cgacggcgcc tatgccgaca cttcggacaa ctacaacctg atcgtccaga agatcggcac
     1261 caacccgcag gccgtgggta tcttcggcta ctcctatctc gagagcaacc tcgacaagct
     1321 caaggccctc accatgggcg gcgtttcgcc gacctacgag accatctcga acttcactta
     1381 tccgggcgcc cgtccgctgt tcatctacgt gaagaaggcg cacctcgaag ccattccggg
     1441 cctcaaggaa ttcgtcgccg agtgggcgaa gagctggggc aaggacggcc cgctcgccaa
     1501 gcagggcctg atcgtgatgc cggccgacaa gctggccgcc agcgccaagg ccgcgagcga
     1561 gttcccctcg ctgaacggcg ccgacctgaa gtaagccaag aaggggctgc gtagaacctt
     1621 gctgtcacca gttgcacctg tcctgctcgt cctcggactc ggcctgatcg gctggctggt
     1681 gacacgtgcc cgggcccgcg gctttgccgc cgggcccgga gccacgtcgg gttccgcgcg
     1741 cccccattcg cttgcgcaat accatgcctg gtacgtggcg ttgtggacgg tggtcccggc
     1801 gctgctgttc gcgctcgtct gggcgagttt ttcgccgggc ctggccctgc accaggcgct
     1861 ctcgagcccg gcggccgcgc agctgccggc cttcggcttc cagcgcgacc ggatcattgc
     1921 cgaggcccag gccgttgcca tgggccaggc gacggcggtg ttcaacccgg cggccagggc
     1981 gctggtcgag ccattccgcg cggcccttgg ccggttcggc ctgatcgggg ccggcatcac
     2041 cctgctgctg gcggcggcag ggggcggcta tgccttcacc aggatcaagg cgcagttcgc
     2101 cgcgcgcaca tcggtcgaac gggcagtcat ggtgctgctg ttcgccgcct cgctggtggc
     2161 tatcttcacc accttcggca ttttcttctc gctgctgttc gagaccatcc ggttcttccg
     2221 catcgtcagc ccggtcgatt tcctgttcgg catgcactgg cagccggatc cgatggccgc
     2281 catcagcccg accacgggcg agaattacgg cgccgtcgcc ctgttctggg caacgatatt
     2341 catcggcgcg gtgatcgcca tggtcgtcgc catcccgatc ggcctgatga gtgcggtgtt
     2401 cctgactcag tatgccagcc cgggcctgcg caaggtcatg aagccgctgc tcgagattct
     2461 cgccggcgtg ccgaccgtgg tctacggtta tttcgctgcg ctgattgtcg ctccggcggt
     2521 gcgcgatttc ggccacgcca tcggcattgc ctcggcctcg agcgagagcg cggccgccgc
     2581 cggcctcgtc atgggcatca tgatcatccc cttcgtctcc tcgatggctg acgacgcgat
     2641 cgccgccgta ccccagtcgc tgcgggacgg cagcctcgcc atgggggcga ccacggccga
     2701 gacgatccgg aaggtgctga tccccgccgc gctccccggc atcgtcgcgg ggatcatgct
     2761 cgcggtcagc cgcgccatcg gcgaaacgat gatcgtcgtc atggccgccg gcgccaccgc
     2821 ccgcctctcg gccaacccct tcgaatcgat gaccacggtg acgtaccaga tcgtcgccat
     2881 gctcaccggc gagggctcgt ttgaccatcc cgccaccctc gccgccttcg ccctcggcat
     2941 ggtcctgttc gtcgtcaccc ttagcctcaa cttcatcgcg ctcagcgtcg tcaagcgcta
     3001 ccgggaagcc tatgactgag ctcacccccc tcgcctcggc cgacaagcag gcgcggctcg
     3061 cagcccggat caagcagcgg caccggtccg atcggctgtt ccgcctggct ggcatgggtg
     3121 ccgtggcctt cgccggcttc gtgctcgcct tcctgctctt caccatgacg gccaatggac
     3181 ttggcggctt tcagcgcgcc gaactcaagg ttcccgtcaa cctcgccaat ggcgtgatcc
     3241 agctcgaccc gtcgcagctg gcctccggca accccgtcgc cgcactggaa agtgccgggc
     3301 tgcccgccct cgtcgacagc gagctggagc gcgcctacgg caaggacggg gcgcagctgg
     3361 tggtcgatgg tgcctggcgc gagctggctc gccgcatcgt gaaagacccg gcgctgctga
     3421 cacatcaggt cgatgcatgg ctgcccgcct ccaatgacct cgccgccgcg ctgcgcggcg
     3481 aaggcacccc ggccgagctg gcccagggcc gggggctggc tcagcagggc gtgctcgcag
     3541 aacatttcga tc
//
LOCUS       DATNBV010000400         2298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_537880,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000400 DATNBV010000000
VERSION     DATNBV010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537880"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VN627_09550"
     CDS             <1..494
                     /locus_tag="VN627_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXD03539.1"
                     /translation="LVDKPGLISSVTEIVRRRGIDCGLLTVEVTETAHLGDRPAAIHN
                     LRQLQALGFRLSIDDFGTGEASLLYLAELPSDELKIDREFVSRVVHHERERHIVSSTV
                     RLAHALGQTVVAEGIEDLATFDLLRKLGCDSGQGYYIGRPETLAQLRARYTAKEQSQR
                     GRV"
     gene            complement(849..1703)
                     /locus_tag="VN627_09555"
     CDS             complement(849..1703)
                     /locus_tag="VN627_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HXD03540.1"
                     /translation="MLVAQITDIHIGFDKGNPDEHNMLRLRAVVDHLVNGPNRPDLLL
                     LSGDLTEDGAPEDYARLVAALAPCPFPIWPMTGNHDDRPTLLNAFPHTPHSPDGFVHY
                     VLDHGALRLILLDTLEPGRHGGGFCEVRASWLAARLAEAPDRPTLVALHHPPFTAGIP
                     WMDTDPREPWVARLAASLAGHPQVKGLVCGHLHRTILSHWEDLPVLVCPSTAPAVGLD
                     LTPLDPDRPDQRKLITDEPPGYCLHWWTGGEVVTHFEYAGDYRTFARFDEKLQPMIRE
                     MIAERPEG"
     gene            complement(1717..>2298)
                     /locus_tag="VN627_09560"
     CDS             complement(1717..>2298)
                     /locus_tag="VN627_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HXD03541.1"
                     /translation="IVMERDCEACHSLVYDKAGPVFRKLRHGDVDQALADVAIAGNTT
                     QPVVTGRARPGEYGANQLYFARFTRPVGGGLVGGMLGKDGICGECHTATVKNGRPAVV
                     PVKLTARYMAHGWFDHASHKQARCTECHAATGSTSSSDVLLPKVAECRTCHIGEQRTG
                     LFAPKGKVPSGCAMCHVYHPTGAAPPLSRHRKS"
BASE COUNT          397 a          697 c          784 g          420 t
ORIGIN      
        1 agctagtcga caagccgggc ctcatcagtt ccgtcactga gattgtccgg cgcaggggca
       61 tcgactgcgg tctgctgaca gtggaagtga ccgaaaccgc ccatctgggt gatcggccag
      121 cggcaatcca caacctgcga caactgcagg ccttgggctt tcgcctttcg atcgacgatt
      181 tcggaacagg cgaggcttcg ctgctctatc tcgccgaact gccgagcgac gagcttaaga
      241 tcgatcggga gttcgtttcg cgtgttgtcc atcatgagcg cgagcggcac atcgtgagta
      301 gcaccgtccg tttggctcat gcgctggggc aaaccgtggt agccgaaggc attgaggatc
      361 tcgctacttt cgaccttctc cgcaaactcg gctgcgattc cggacaaggc tattatatcg
      421 gtcgccccga aaccctggcg cagttgcgcg cccgctacac cgcaaaggag cagagtcaac
      481 gcgggcgggt ttaaccactt gttaaccacg aaagctggtg ttaaccatgg gccttcggaa
      541 atccgaagga ggctcatatg ttgtggtggc tggctttttg gccgactaac aagtaatatc
      601 ccgtgaatcc tcggttttcc ggggcattgc ggaacagggg ctaatgcgaa tcagtcggag
      661 tgcgaagcac ctgatccgat tcggattggt taataatggg gggtgaaatc ccctccgaca
      721 ggtgccccgg acctccgggg cacttttctt ttgggctgac gacaccgttt tttcggtgga
      781 cccgagcatt gcgtggttgg acacgtggga acgccagcct gcctgagcgg ccggcggcac
      841 cgagataatc agccctcagg ccgctcggcg atcatttcac ggatcatcgg ctgcagcttt
      901 tcatcgaatc gcgcaaaggt ccggtagtcg ccggcatatt cgaagtgggt gacgacttcg
      961 ccgcccgtcc accagtggag gcagtagccc ggcggttcgt cggtgatcag cttgcgctgg
     1021 tcggggcgat cggggtccag tggggtaagg tccagtccca cggccggtgc ggtcgagggg
     1081 cagacgagta ccggcaggtc ctcccagtgg gacaggatgg tgcggtgcag gtgcccgcag
     1141 accaggccct tgacctgagg gtgcccagcc aggctcgccg caaggcgcgc cacccatggc
     1201 tcgcgcgggt cggtgtccat ccacgggatg ccggcggtga acgggggatg atgcagggca
     1261 accagcgtcg gccggtcggg cgcctcggcc agccgcgccg cgagccagga cgcgcggacc
     1321 tcgcaaaagc cgccgccgtg gcgtcccggt tccagcgtat ccagcagaat cagtcgcagc
     1381 gcgccgtgat cgagcacgta atgcacgaaa ccatcgggcg aatgcggtgt gtgtgggaag
     1441 gcgttcaaga gggtcgggcg atcgtcgtgg ttgccggtca tcggccagat cgggaagggg
     1501 cagggggcca gggccgcaac cagccgcgca taatcttccg gggcgccgtc ctccgtgaga
     1561 tcgccgctca gcagcagcag gtcgggccgg ttggggccat tcaccaggtg atcgaccacc
     1621 gcgcgcaggc gcagcatgtt gtgctcatcg ggattgccct tgtcgaaccc gatgtggatg
     1681 tcggtgatct gcgcgaccag catcccgttc tcccgatcag ctcttgcggt ggcgggagag
     1741 cggcggggcc gctccggtgg gatggtagac gtggcacatg gcgcagcccg aggggacctt
     1801 gcccttgggg gcaaacaagc cggtgcgctg ttcgccgatg tggcaggtgc ggcattcggc
     1861 caccttgggc agcagcacgt cgctcgacga agtagagccg gtcgccgcgt ggcactccgt
     1921 gcacctggcc tgcttgtgag aggcatggtc gaaccagccg tgtgccatgt agcgggccgt
     1981 cagcttcacg ggcacgaccg ccggacggcc gttcttcacc gtggcggtgt gacactcccc
     2041 gcagatgccg tccttgccga gcatgccgcc gacaagcccg ccgcccaccg gccgggtgaa
     2101 ccgggcgaag tagagctggt ttgcgccata ttcgccgggt cgagcgcggc cggtgacgac
     2161 gggctgggtg gtgttgccgg cgatcgccac atcggcgagc gcctgatcca cgtccccatg
     2221 gcgcagcttt cggaacaccg ggccggcctt gtcatagacc aggctgtggc aggcctcgca
     2281 atcgcgctcc atgacgat
//
LOCUS       DATNBV010000401         4167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2788973,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000401 DATNBV010000000
VERSION     DATNBV010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2788973"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2051)
                     /locus_tag="VN627_09565"
     CDS             complement(<1..2051)
                     /locus_tag="VN627_09565"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXD03542.1"
                     /translation="MPNTIQELLLRLSPICLLLAGTGLSPAVAFADEAVPSANAAPTV
                     TPGSAAPTTPGETSEDERLEPQAGEIVVVATRIKGQVDAPQPPIQVLDEEDIASYGAA
                     SITDLLDALSPQTGSGRGRGSGRPVVLLNGQRISGFREMRNIPPEAIRRLEILPEEVA
                     LRYGYPADQRVINFILKDNFAAKTVEVEYGRPTRGGTDTSKLEGSLFRTSGQSRLNLT
                     VTADDTTPLFENERGVIQAPGSVPTVAGDPDPASARTLIADSRDLGVNATWTKGLGEK
                     GLAGSITVNGAYTRADSTSFSGLSLAADPTGAIVRSFGDPLARRTKSDTAEAGLTYAR
                     GLGEWQLTATVDASHAITNTTVDQRSVLTGPLPATGPVSFTTAAPALARSVSDSATSL
                     VTAVGRPLHLPAGDVTATLKAGFAWTGIASSDTRNAGGETRLRRGDASAGINLGLPVA
                     SRRNQVLAGIGDLSLNFSAGVDRLSDIGTLTDWSAGLTWGITESLGLQASYIVNQVAP
                     GLTDLGNPVVQTFNVPVYDFTRGQTALVTVTSGGNPLLARETDRDVKLGLTWQLPFLQ
                     RSNFIVEYFHNNSSNVTAGFPLLTPAIEAAFPGRVTRDSAGRLIGIDRRPVTLANQTS
                     SRLRYGFNLAGPLGKPRAGASGGGRFGGGGFGGSPPGGGMDGHSPGVSGSGFGGGRGG
                     RG"
     gene            2287..2532
                     /gene="infA"
                     /locus_tag="VN627_09570"
     CDS             2287..2532
                     /gene="infA"
                     /locus_tag="VN627_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXD03543.1"
                     /translation="MAKEELLEMRGQVVELLPNAMFRVRLENDHEILGHTAGKMRKNR
                     IRVLVGDEVLVELTPYDLTKGRITYRFMPGRGGPGGY"
     gene            2687..3265
                     /locus_tag="VN627_09575"
     CDS             2687..3265
                     /locus_tag="VN627_09575"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HXD03544.1"
                     /translation="MPESPLLILASASPRRRDLIGRLGIEPLRISAADIDETPRKAEA
                     PRDYARRMAREKALAVPAQDAHVLAGDTVVACGRRILPKAEDEATARRCLELLSGRRH
                     RVLSAIALNGPGGTLRERLSETIVRFKPLSEAEIAAYLAGGEWHGKAGGYAIQGSAEG
                     LIAWIGGSHSGVVGLPLFETRALLKAAGFAIA"
     gene            3258..>4167
                     /locus_tag="VN627_09580"
     CDS             3258..>4167
                     /locus_tag="VN627_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HXD03545.1"
                     /translation="MPEWLVEEGIAEHRAILTDHGEVLAARIDWPGRLAPGAVADAVL
                     LHRTAGSPRGTARFSSGEQALVDRLSREASEGAPLRLAITRAAMAEHGRHKLAQARPT
                     TDPVRPAPTLAERLRADGQQVRTVRRLGSEWDELWAEAWSGELAFTGGAISVTPTPGL
                     TVIDVDGTLPAPALARAAAPAVAAAIRRFDLAGSIGIDFPTLEAKADRKAVDEALGAA
                     LHGWRHERTAMNGFGFVQLVARLERPSLVHRLALDRPGAAARLLLRRAEAVAEPGPRL
                     QLTCHPAVRAAIRPEWEADLARRTART"
BASE COUNT          617 a         1569 c         1344 g          637 t
ORIGIN      
        1 ccccggccgc cacgcccgcc gccgaacccg ctgccgctta cgcccggcga atggccatcc
       61 atgccgccgc ccggcggcga tccaccaaat ccgcctccgc caaatcgccc gcccccgctg
      121 gcgccagccc tgggcttgcc gagcggtccg gcgagattga aaccataacg caggcgcgag
      181 ctggtctggt tggcaagcgt cactggcctt cggtcaatcc cgatcagccg acccgcggaa
      241 tcgcgcgtca cccggccggg gaaggccgcc tcgattgccg gcgtgagcag cgggaatccc
      301 gccgtcacgt tgctcgaatt gttgtggaag tactcgacga tgaaattcga gcgctggaga
      361 aacggcaatt gccaggtcag cccgagcttc acgtcacggt ccgtctcgcg cgccagcaag
      421 ggattgccgc cgctggtcac agtcaccagc gcggtctgcc cgcgggtgaa atcgtagacc
      481 ggcacgttga aggtctgcac caccggattg ccgaggtcgg tcagcccggg cgccacctgg
      541 ttgacgatgt agctcgcctg cagcccgagg ctttcggtaa tcccccaggt cagcccggcg
      601 ctccagtcgg tcagggtgcc gatgtcggac aggcggtcca ccccggcgct gaaattgagc
      661 gagaggtcac cgatcccggc cagcacctgg ttgcggcggc tggcgacagg caggccaaga
      721 ttgatcccgg ccgaggcatc gccgcgccgc agccgggtct cgccgccggc attgcgcgtg
      781 tcgctgctgg cgatgccggt ccaggcaaat cccgccttga gcgttgcggt tacatcgccc
      841 gccggcaggt gcagcggccg gcccacggcg gtgacgaggc tcgtcgccga atcgctcacg
      901 ctgcgggcca gcgccggggc agccgtggta aagctcaccg gccccgtcgc cggcagcggc
      961 cccgtcagga cggagcgctg gtcgaccgtg gtattggtaa tcgcatggct cgcatcgacc
     1021 gtggctgtca gctgccattc gcccagtccc cgggcatagg tcagcccggc ttcggccgtg
     1081 tcgcttttcg tgcgccgcgc cagcggatcg ccgaagctgc ggacaatggc tcccgtcggg
     1141 tccgcggcaa gactgaggcc cgaaaagctg gtcgaatccg cgcgcgtgta ggccccattg
     1201 acggtgatcg atccggccag gcccttctca cccagccctt ttgtccatgt agcattgacc
     1261 cccaagtccc ggctatcggc aatcagcgta cgcgccgagg cgggatccgg gtcccccgcc
     1321 acggtcggca cgctccccgg cgcctggatc acgccgcgct cgttctcgaa cagcggcgtg
     1381 gtatcgtccg ccgtcaccgt caggttgagc cggctctgcc cggacgtgcg gaacagcgat
     1441 ccttcgagct tgctggtatc cgtgccgccc cgcgtcggcc ggccgtattc gacctccacc
     1501 gtcttggcgg cgaaattgtc cttgaggata aaattgatca cccgctggtc ggcggggtag
     1561 ccatagcgca gcgccacttc ctcgggcagg atttccagcc gccggatcgc ctcgggcggg
     1621 atgttgcgca tttcgcggaa gcccgagatc cgctggccgt tgagcaggac caccggccgg
     1681 ccgctgcccc gcccgcgtcc cgaccccgtc tgcggcgaga gcgcatcgag cagatcggtg
     1741 atcgaagcgg cgccgtagga ggcgatatcc tcctcgtcca gaacctggat cggcggctgc
     1801 ggcgcatcca cctgcccctt gatccgggtg gcgacgacga cgatctcgcc cgcctggggc
     1861 tccagccgct catcctccga tgtctcgccc ggcgtggtcg gcgcggcgct gccgggggtg
     1921 accgtggggg cggcgttggc cgaggggacg gcctcgtcgg cgaaagccac ggcgggggac
     1981 aggccggtgc cggccagcag caggcagatc ggggaaaggc gcaagagcaa ctcctgaatg
     2041 gtattcggca tccgtcccca ctcttcgaga tgcccctccg ggtttcccgg gacaagccgc
     2101 tcccgggtaa caaattgtcc cgccgctgat acctttgccg cgtccgcgcg ttcccgcccc
     2161 aggcccgcgg cgtgtgcgcg gcaagcccac ccgccggaaa ggcttccctt ttgggccgcc
     2221 cccgcgtatg ggcccggcgg attgtcggcc tgagaggcgg ctcaacgaaa cggaccagaa
     2281 tagcggatgg ccaaggaaga actgctcgag atgcgcggcc aggtggtcga actcctcccc
     2341 aacgcgatgt tccgcgtgcg gctggaaaac gatcacgaga tcctcggcca cactgccggc
     2401 aagatgcgca agaaccgcat ccgcgtgctg gtgggcgatg aagtgctggt cgagctgacc
     2461 ccctatgacc tgaccaaggg ccgcatcacc taccgcttca tgccaggccg cggcggaccc
     2521 ggcggctact gaatgctggg tgtttcggcc catgccgggc tgcaaacggc cgccttctgc
     2581 gcttctggtg ctcacgacct tcacggtcgc tgcgcgccgg ttctcgaaga cagctatttt
     2641 cgctccggcc tgagccaaaa caccccacat tcagcctctc ccgcagatgc ctgaatcccc
     2701 ccttctcatc ctcgcctccg cttcgccccg ccgccgtgac ctgatcggac ggctgggcat
     2761 cgagccgctg cgcatttccg ccgcggatat cgacgaaacc ccgcgcaagg ccgaggctcc
     2821 gcgcgactat gcccgccgca tggcccgcga aaaggcgctc gccgtacccg cccaggatgc
     2881 ccacgtgctg gccggcgata cggtcgtcgc ctgcggccgc cgcatcctgc ccaaggcgga
     2941 agacgaagcc accgcccggc gctgcctcga actgctctcc ggccgccgcc accgcgtcct
     3001 atcggccatt gcgctgaacg gcccgggcgg caccctgcgc gaacgtcttt ccgaaaccat
     3061 cgtccgcttc aagccgctca gcgaagccga gatcgccgcc tatctcgcgg gcggcgaatg
     3121 gcatggcaag gcgggcggct atgccatcca gggcagcgcc gaggggctga tcgcgtggat
     3181 cggcggcagc cattcgggcg tcgtcggcct gcccctgttc gaaacgcggg ccctgctcaa
     3241 agccgccgga ttcgccattg cctgagtggc tggtcgagga agggatcgcc gaacaccgcg
     3301 cgatcctcac ggaccacggc gaagtcctcg ccgcgcggat tgactggccc ggccgcctcg
     3361 ctccaggcgc agtcgctgat gccgtgctcc tccaccgcac cgccggttcc ccgcgcggca
     3421 ccgcccgctt ctcctcgggc gagcaggcgc tggtcgatcg gctctcgcgt gaggccagcg
     3481 aaggcgctcc cctgcgcctc gccatcaccc gcgccgccat ggcggaacat ggccgccaca
     3541 agctcgccca ggctcgcccc accaccgatc cggtccgccc tgcgccaacc cttgccgaac
     3601 gcctgcgcgc cgacggccag caagtccgca cagtccggcg cctcggctcc gaatgggacg
     3661 agctgtgggc cgaggcctgg tcgggcgaac tcgcctttac cggcggcgcg atctccgtca
     3721 cgcccacccc gggcctgaca gtgatcgatg tcgacggcac actgccggcc cccgccctcg
     3781 cccgcgccgc cgccccggcg gtcgccgctg cgattcgccg cttcgacctg gccggctcaa
     3841 tcggcatcga ttttcccacg ctcgaggcca aggccgatcg caaggcggtc gacgaagcgc
     3901 tcggcgccgc gctccacggc tggcggcacg aacgcacggc gatgaacggc ttcggcttcg
     3961 tccaactcgt cgcccggctc gaacgcccct cgctggtcca ccgcctcgcg cttgaccgcc
     4021 ccggcgccgc cgcccgcctg ctcctgcgcc gcgccgaagc cgtggctgag ccgggcccgc
     4081 gccttcagct cacctgccac cccgccgtgc gggccgcgat caggcccgaa tgggaagccg
     4141 atctcgcccg gcgcaccgcc cgcacgc
//
LOCUS       DATNBV010000402         3853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2788987,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000402 DATNBV010000000
VERSION     DATNBV010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2788987"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VN627_09585"
     CDS             <1..226
                     /locus_tag="VN627_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03546.1"
                     /translation="NSTCSMALSRNLCAVTEPLVAGTMKGKPAAWQFALGDNRLAGAR
                     YLDNEGPLGGNGKQRTTRRAMRLAALADSP"
     gene            236..985
                     /locus_tag="VN627_09590"
     CDS             236..985
                     /locus_tag="VN627_09590"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HXD03547.1"
                     /translation="MSGEIVDNRGRGTPAWRWPSIHPEGRKFGLIVAAASLVFAFLAW
                     ETVAWPLGFLTLGVLAFFRDPERVTPQGDKFVVSPADGLVTLIQKVEPPRELVLDDGA
                     GTPSLRAGPVTRVSIFMSVFDVHINRAPIGGTVRRVVYIPGRFLNADLDKASEENERQ
                     HLLVERSDGTMIGFTQIAGLVARRIVPFVKPGDILAAGQRVGLIRFGSRVDVYLPAGT
                     EPKVLMGQKVVAGETILAEIGQDPLIEGVCQ"
     gene            1039..1902
                     /locus_tag="VN627_09595"
     CDS             1039..1902
                     /locus_tag="VN627_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HXD03548.1"
                     /translation="MGAAERPGAVRLPGGLTLRALIPNAITASALCFGLTGIRFAITA
                     ASGQPADPAAMTSGLPLDDWQKAVLAVLLAGVLDGIDGRIARLLKAQSRFGAELDSLS
                     DAISFGVAPALILFLWSLQDLPRLGWFAALAFAICCVLRLARFNARIDMVDQPHKSAG
                     FLTGVPAPAGAGLAFLPLFLWLATGREEFRDPLFVSGWLVLIAFLLISSLPTLSWTSL
                     RPRRSVRLELIAAIALVCAALLTEPWLTLVAIDAAYLLLIPYGVWRYARVRRQRAVQT
                     TAAPEPSPPAP"
     gene            complement(1841..2119)
                     /locus_tag="VN627_09600"
     CDS             complement(1841..2119)
                     /locus_tag="VN627_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03549.1"
                     /translation="MIATLASFALPCLFIGSAVFALATLALTWRAYGRELGALRAQLA
                     ACPDLREYQGRVATTRVHEFTSGLRRSAIRARAGSAPARRLSGRRAVA"
     gene            2487..3239
                     /gene="rpsB"
                     /locus_tag="VN627_09605"
     CDS             2487..3239
                     /gene="rpsB"
                     /locus_tag="VN627_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HXD03550.1"
                     /translation="MAAPVVTMNQLIEAGAHFGHQTHRWNPRMKPYIFGARNGVHILD
                     LSQTVPLFARALEFVDATVRAGGKVLFVGTKRQAQQPIAEAARQCGQHFVNHRWLGGM
                     LTNWKTISGSIKRMKALEEQLAGDTAGLTKKEVLQLTRERDKLELSLGGIRDMGGIPD
                     VMFVIDANKEELAIKEANVLGIPVVAILDSNVSPDGIAFPIPANDDASRAIRLYCETV
                     AAAATKGRREGVVDSGVDVGALDNPVEEALEG"
     gene            3348..>3853
                     /gene="tsf"
                     /locus_tag="VN627_09610"
     CDS             3348..>3853
                     /gene="tsf"
                     /locus_tag="VN627_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXD03551.1"
                     /translation="MAYTAADVKNLRERTGAGMMDCKKALDESNGDFEAAVDALRAKG
                     LAAVAKKSSRTAAEGLVGVAVSGTRGVAVEVNSETDFVAKNDQFQDFVRKTTEVALGA
                     SSDDIEALKASAYPGGGTVADTLTNNVATIGENQQVRRMKTVSVSQGVVVPYMHNAAA
                     PNLGKIGVL"
BASE COUNT          610 a         1336 c         1236 g          671 t
ORIGIN      
        1 gaactccacc tgctccatgg cactctcccg aaatctgtgt gcggtcacgg agccgctcgt
       61 cgccggcact atgaaaggca aaccggcggc ctggcaattc gctcttggcg ataaccgtct
      121 ggcgggggcc aggtatctgg acaacgaagg gccgttgggc ggcaatggca agcagcggac
      181 gacccggaga gccatgcgcc ttgccgcctt ggccgactcc ccctagatga acgccatgtc
      241 cggagaaatc gtcgataacc gcggccgcgg gactcccgcc tggcgctggc ccagcattca
      301 tcccgaaggt cgcaagttcg gcctcatcgt cgcagcggcc tcgctggtct tcgccttcct
      361 ggcgtgggaa accgtggcct ggccgctggg ctttcttaca ttgggggtgc tcgccttctt
      421 ccgcgatccc gaacgcgtta cgccgcaggg cgacaagttc gtggtctcgc cggcggatgg
      481 cctcgtcacc ctgatccaga aggtcgagcc gccccgcgaa ctggtgctcg atgatggcgc
      541 cggaacgccg tcgcttcgcg ccggcccggt cacccgcgtc tcgatcttca tgagcgtgtt
      601 cgacgtgcac atcaaccgcg cgccgatcgg cgggaccgtg cgccgcgtgg tctacatccc
      661 cggccgcttc ctcaacgccg atctcgacaa ggcgagcgag gagaacgagc gccagcacct
      721 gctggtcgaa cgcagtgacg gaaccatgat cggcttcacc cagatcgccg gcctcgtcgc
      781 ccgccggatc gtgccgttcg tcaagccggg cgatatcctc gccgccggtc agcgcgttgg
      841 cctgatccgg ttcggcagcc gggtggacgt ctacctgccg gccgggactg aaccgaaggt
      901 tctgatgggc cagaaggtcg tcgccgggga aaccattctt gccgaaatcg gccaggaccc
      961 gctgatcgaa ggcgtttgcc agtgagcgac gaccggccgg gctacgttcc gccttcgcgc
     1021 ccgccgtcgc gcgcgcgcat gggcgcggcc gagcggcccg gcgcggtccg cctgcccggg
     1081 gggttgaccc tgcgcgcgct gattcccaat gccatcaccg cctcggcgct gtgcttcggc
     1141 ctcaccggta tccgcttcgc catcacggct gcttccggcc agccagcaga tcccgccgca
     1201 atgacaagcg gcctgccgct ggacgattgg cagaaggcag tgcttgccgt gctcctcgcc
     1261 ggtgtgctcg acgggatcga cggacgcatc gcccgcctcc tcaaggccca gtcacgcttc
     1321 ggcgccgagt tggacagcct gtcggacgcg atctccttcg gcgtcgcccc ggcgctgatc
     1381 ctgttcctgt ggtccttgca ggacctgccg cgactcggtt ggttcgcggc gctggccttc
     1441 gccatctgct gcgttctccg gctcgcccgg ttcaatgccc ggatcgacat ggttgatcag
     1501 ccgcacaagt ccgcgggttt cctcaccggg gttccggccc ccgcgggcgc cgggctggcc
     1561 ttcctgccgc tgttcctgtg gctggcgacg gggcgcgaag agtttcgcga tccgctgttc
     1621 gtcagcggct ggctggtgct gatcgccttc ctgctgatct ccagcctgcc gaccctgtca
     1681 tggacctcgc tgcgcccgcg ccgctcggtc cgtctcgaac tgattgcggc gatcgcgctg
     1741 gtctgcgccg ccctgcttac cgagccatgg ctgacgctgg tggcgatcga tgcggcctac
     1801 ctgctgctga ttccctatgg cgtctggcgc tatgccaggg tcaggcgaca gcgcgccgtc
     1861 cagacaaccg ccgcgccgga gccgagcccg cccgcgccct gatcgcgctg cggcgcaggc
     1921 ccgaggtgaa ttcgtgtacc cgggtggtgg ctacccggcc ctggtattcg cgcagatcgg
     1981 ggcaagcggc cagctgggcg cgcagcgcgc cgagttcgcg gccataggcc cgccaggtca
     2041 gcgcaagggt ggcgagggcg aaaacggcgc tgccaatgaa caggcagggc aaggcgaagc
     2101 tggcgagcgt tgcgatcatc actcaagcgt ccttttccgg ccggccttgg caggcgggcc
     2161 acttgcttcc ttaaacgggc ggcaactcga gcgttcccgc ttcggcttat gatcaccata
     2221 tgttccatct gtgttccgct cgtcaagcgc taaattccgg ttttgttcta ttttggagtg
     2281 atctcccaaa tagcgctgga atttcagccg caggggcggt aagggccgcg cgtccgcgga
     2341 ggcggcgtgc agcctgcgcg ccttctcgcc gcgggcaaat ccacacggga agcactcatc
     2401 ccggtgcctc agcggatctc acgccccttc gggggttccc cgcaaggttc cagcttcccg
     2461 gaggcccaac cggaaaggaa agtcctatgg cggctcctgt cgtcaccatg aatcagctca
     2521 tcgaagccgg cgcccacttc ggccaccaga cccaccgctg gaacccgcgg atgaagccgt
     2581 acatcttcgg cgcccgcaac ggggtccaca tcctcgacct gtcgcagacc gtgccgctct
     2641 tcgcccgcgc cctcgaattc gtcgacgcca cggttcgcgc tggcggcaag gttctgttcg
     2701 tcggcaccaa gcgtcaggcc cagcagccga tcgccgaggc ggcccgccag tgcggccagc
     2761 acttcgtcaa ccaccgctgg ctcggcggca tgctgaccaa ctggaagacc atctcgggct
     2821 cgatcaagcg catgaaggcg ctggaagagc agctagcggg cgacaccgcc ggcctcacca
     2881 agaaggaagt cctccagctc acgcgtgagc gtgataagct cgagctgtcg ctcggcggca
     2941 ttcgcgacat gggcggcatt cccgacgtga tgttcgtgat cgatgccaac aaggaagagc
     3001 tggcgatcaa ggaagccaac gtgctcggca tcccggtcgt cgcaatcctc gattctaacg
     3061 tctcgcctga cggcatcgcc ttcccgatcc cggcgaacga cgacgccagc cgtgccatcc
     3121 ggctctactg cgaaaccgtc gccgccgccg ccaccaaggg ccgccgcgag ggtgtggtcg
     3181 attcgggcgt cgatgtcggc gcgctcgaca atccggtcga agaagctctc gagggctgat
     3241 tcccgcaggc ggcttgccgg gttggccggg gtcgcctgtc atcaaattga agcgcgccgg
     3301 gcctttgggt ccggcgccgt tccatttcag aacaaggaac ccaagcgatg gcctataccg
     3361 ccgctgacgt gaagaacctg cgcgagcgca ccggcgccgg catgatggat tgcaagaagg
     3421 cgctcgatga gagcaatggc gatttcgaag ccgcggttga cgcgctgcgc gccaagggcc
     3481 tcgccgctgt cgccaagaag tccagccgca cggccgctga aggcctggtc ggcgtcgccg
     3541 tctccggcac tcgcggcgtg gccgtggaag tgaactcgga aaccgacttc gtcgccaaga
     3601 acgaccagtt ccaggacttc gtccgcaaga cgaccgaagt cgccctgggt gcctcgtcgg
     3661 acgatatcga agctctcaag gcctcggcct atccgggcgg cggcacggtg gccgacacgc
     3721 tgaccaacaa cgtcgccacc atcggtgaga accagcaggt tcgccgcatg aagaccgttt
     3781 cggtcagcca gggcgtggtc gttccctaca tgcacaatgc cgccgcgccg aacctcggca
     3841 agatcggcgt gct
//
LOCUS       DATNBV010000403         4180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_244572,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000403 DATNBV010000000
VERSION     DATNBV010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244572"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VN627_09615"
     CDS             complement(<1..961)
                     /locus_tag="VN627_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXD03552.1"
                     /translation="MKVDEASTSNAASSCHGGQRANDMSREPVNAVPLDVRAIRGHFG
                     FPELGRVVTNNAASTQPPAELLALYASLGPGYENVHRGQSSASQAMTALFEESYDTIA
                     QFVGAPSRASVALYRNTTEAINAVMYSLLTEFRDGDNVVTTLMEHNSNYVPWYGLCRE
                     ILPRLGRRVQCRLARFDPVTGELDLGHLASLIDARTKLVCCTGASNFLGTRPPLDKVR
                     ALAGASGYHQPNGERRSYLLVDGAQLVPGSFVDVRALDVDFLAFSFHKLLAPFGVGVL
                     YGKEHLLEAMLPFLYGGDMIAEGQVFPDKVGYNALPWKYAAGTP"
     gene            1251..4121
                     /locus_tag="VN627_09620"
     CDS             1251..4121
                     /locus_tag="VN627_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXD03553.1"
                     /translation="MNGHAITISGMVAVAEAKPLLLERDVELARLSALLGNAQAGSGA
                     VVAIGGPAGIGKTELLAAVHRFAGDRGFRSLRARGRELEAGMAFSVMRQLLEQPVLSA
                     SAGERRRLLAGPARAGAGALGLAAGDAPASEFAAVHGLYWLCANLADRQPLLLTVDDL
                     QWVDGPSLSWLAYLGPRAAESSMLVVLTVREGDPRGRANSVAAGADDSSVHRMGLSAL
                     SVASVAALVHAELGPAASAGFCSACWELAGGNPLYVRELLAAACTEGLSGAEDDVTAL
                     RALASSAVGALVLGRLARLGGDAVSLARALAVLGSQTEVGVAAELAGLDPAAAELTAD
                     GLAAAQILAPARPLDFFHPVIGEAVYADLALGERRLAHRRAAAILDRAGAADRVAAHL
                     LATGPAGEAWVVERLSAAAATAGERGAAEVAASYLRRAVAEPAAPAERPALLRRLGVA
                     EWAAGEPAAIGHLEEALNEARDATAIGAASGPLANAYLISDQADIAVAVLQRAIARIR
                     PTDPRLALRLDGAAALFGLMDDRTAPAALRMVDRLQASLGETADPPARVLVAIAHAEM
                     RRAQAADEAERLLERALAREAYPPRPNASAAIIVTLMGLEDFGTLQRLCDDMLSAARR
                     RSAVQDLIGIASFSAWALYRCGELADAEAQARWALEHASGIWAIDALAHLVETLVERD
                     ALDDAAAELQQMAPPVTSHSVLVGNYLMARGQLRMAQGRREEALQDFLACGERCAPLG
                     MVDGLYEWRSAAAIAHALLGRAAEARRLARSAVKVMRGFGRPRALGVALRAEGLAEGG
                     DRGIGLLGEAVAVLERSQAQVELARALTDHGAALRRAGHRSKARAQLERGLDLAHHCG
                     ARRIAGQARAELIAAGAKPRRDAITGRDALTASELRVARLAAAGKSNREIAQELFITT
                     KTASAHLSRAYRKLDVTRRNQLAEALVSKTPAVSRHEATRTA"
BASE COUNT          565 a         1477 c         1549 g          589 t
ORIGIN      
        1 taggcgtgcc ggccgcgtac ttccacggca gcgcgttgta gcccaccttg tccgggaaca
       61 cctgcccctc ggcgatcatg tcgccgccgt acagaaacgg cagcatcgcc tccagcagat
      121 gctccttgcc gtacagcacc cccaccccga acggcgccag cagcttatgg aacgaaaacg
      181 ccaaaaagtc cacatcgagc gcccgcacgt caacgaacga ccccgggacc agctgcgccc
      241 cgtccaccag caggtacgac cggcgctccc cgttcggctg gtgatatccg ctggcaccgg
      301 ccagcgcgcg gaccttatcc agcgggggcc gggtgcccag gaagttcgag gcgccggtgc
      361 agcacaccag cttggtccgc gcgtcgatca gcgacgccag atggcccagg tccagctccc
      421 cggtcaccgg gtcaaatcgg gccagccggc actgcacccg ccgccccagc cgcggcaaaa
      481 tctcccggca cagcccatac cacggcacgt agttcgagtt gtgctccatc agcgtcgtca
      541 ccacattgtc gccgtcgcgg aactcggtca gcagggagta catgaccgcg ttgatcgcct
      601 cggtggtgtt ccggtacaag gcaacgctgg cccggctggg cgccccaaca aactgggcga
      661 tggtgtcgta ggactcctcg aacagcgcgg tcatcgcctg cgaggcgctg gactggcccc
      721 ggtgcacgtt ctcatacccc ggcccgagcg aggcgtacag cgccagcagc tcggccggcg
      781 gctgcgtgct cgccgcgttg ttcgtcacca cccgccccag ctcggggaac ccgaaatgcc
      841 cacgaatggc ccggacatcc agcgggacgg cgttcaccgg ttcgcggctc atgtcgtttg
      901 cccgctgccc tccgtggcag ctgctggccg cgttgctggt gctggcttcg tcaactttca
      961 tcgcgggtac cccccgtcct ccgcggaacg tcggctcgcc gccgacccgg ttcgcgtcgc
     1021 atcgccccgt ccgctggtag cggatgatcg agctgacccg agtctgcaag ccagcaggtc
     1081 gggcggcatc gggcacgcca tccccgtctt ttgccgccca tgccttagga aatccgtgcc
     1141 tctgtgggtc acgggcgctg gcggggacgc cctggatcta tgcggcgagc gccgcatcac
     1201 gaggccgcag atggtcaagg atctaccagt ggccagtcac atgcttacca atgaacggtc
     1261 atgcgattac catcagcggc atggtggctg tcgccgaggc taagccgctg ctgctggaac
     1321 gggatgtgga gctggcgcgg ctgagtgcgc tgctcgggaa cgcgcaggcg ggcagcgggg
     1381 cggtggtagc catcggcgga ccggccggga tcgggaagac ggagctgctg gcggcggtgc
     1441 accggttcgc cggggaccgg gggttccggt cgctgcgggc ccgcggccgt gagcttgagg
     1501 cgggcatggc gttctcggtc atgcgccagc tgctggagca gccggtgctg tcggcctccg
     1561 ccggtgagcg gcgccggctc ctggccggcc cggcccgggc cggggcgggc gcgctgggcc
     1621 tcgcggccgg tgatgcgccc gcctccgagt tcgcggcggt acacggcctg tactggctgt
     1681 gcgcgaatct cgccgaccgc cagccactgc tgctgacggt agacgatctg caatgggtcg
     1741 acggtccctc gctgtcgtgg ctggcctatc tcgggccgcg cgccgccgag tcctcgatgc
     1801 tggtggtcct gaccgtgcgc gagggcgatc cccgcgggcg ggccaactcg gtcgcggcgg
     1861 gggcggacga ctcctcggtg caccgcatgg gactgtcggc gctgagcgtg gccagcgtcg
     1921 ccgcgctggt ccacgcggaa ctcgggcccg cggcgagcgc ggggttctgc tcggcctgct
     1981 gggagctggc gggcggtaac ccgctgtatg tgcgggaatt gctggccgcg gcgtgcaccg
     2041 agggactgtc cggtgcggag gatgacgtaa cggccctgcg cgccctcgcc tcgtccgcgg
     2101 tcggggccct ggtgctcggc cgcctggccc ggctgggcgg cgatgcggtg tcgctggcgc
     2161 gggccctggc ggtgctcgga tcgcagaccg aggtcggggt ggccgccgag ctggccggcc
     2221 tggatccggc cgccgcggaa ctgacggccg acggcctcgc ggcggcgcag atcctcgcgc
     2281 cggcacggcc actcgacttc ttccatccgg tgatcggcga ggccgtgtac gccgatctgg
     2341 cgctgggcga gcggcgactg gcgcatcggc gggccgccgc gatcctcgac cgggccgggg
     2401 cggccgaccg ggtcgctgcc cacctgctgg ccaccgggcc ggccggcgag gcatgggtgg
     2461 tggagcggct gagcgcggcc gccgccacgg ccggagagcg cggcgcggcc gaggtcgcgg
     2521 cgagctatct gcggcgggcc gtggccgagc ccgcggcgcc ggccgagcgc ccggcgctcc
     2581 tgcggcggct gggcgtcgcg gaatgggcag caggcgagcc cgcggcgatc ggccacctcg
     2641 aagaggcgct gaacgaggcg cgcgacgcaa cggcgatagg cgcggcgtca gggccgctgg
     2701 ccaacgcgta cctcatctct gaccaggcgg acatcgcggt cgcggtgctg cagcgagcca
     2761 tcgcccggat acggcccaca gacccgcggc tggccctgcg gctggatggc gcggcagcgc
     2821 tctttggcct gatggacgac cgtactgcgc ccgccgccct ccgcatggtg gaccggctgc
     2881 aggccagcct tggtgagacg gccgacccgc cggctcgcgt gctcgtcgcc atcgcacacg
     2941 cggagatgag gcgcgcccag gccgcggatg aggccgagcg gttgctggaa cgagcgctcg
     3001 cgcgcgaggc ctaccccccg cggcccaacg cctccgccgc gatcatcgtg accttgatgg
     3061 gactggagga cttcggcacg ctgcaacggc tgtgtgacga catgctgagc gcagcgcggc
     3121 ggcgttcggc cgtgcaggat ctgatcggga tcgcgagctt ctcggcctgg gccctgtacc
     3181 ggtgtggcga gctcgccgac gccgaggctc aggcgcgctg ggcgctcgag catgcctccg
     3241 ggatctgggc gatcgacgcg ctcgcgcacc tggtcgagac gctggtcgag cgcgatgcac
     3301 tggacgacgc ggccgccgag ctgcagcaga tggcaccacc ggtgacctcc cattcggtcc
     3361 tggttggcaa ctacctgatg gcgcgcggcc agctgcgcat ggctcaggga cgacgcgagg
     3421 aggcgctgca ggacttcctg gcctgtggcg agcgatgcgc accgctcggg atggtcgatg
     3481 gcctctacga atggcgctcc gcggcagcga tcgcgcatgc tttgctcggc cgcgcggctg
     3541 aggcccggcg gctcgcccgc agcgcggtca aggtgatgcg cggattcggg cggccccggg
     3601 ctctgggtgt cgcgctgcgc gccgagggcc tggccgaggg cggcgaccgc ggcatcggcc
     3661 tgctcggcga ggcggtcgcg gtgctcgagc gctcgcaggc gcaggtcgag ctggcccgcg
     3721 cgctcaccga ccacggtgcg gcgctccgcc gggcgggcca ccgcagcaag gcgcgggctc
     3781 agctggagcg cggacttgac ctggcgcacc actgtggcgc gcgccgcatc gctggccagg
     3841 cccgggcgga gctcatcgcg gcgggcgcca aaccgcggcg cgacgcgatc accggccgcg
     3901 acgccctgac cgccagcgag ctgcgggttg cccggctggc ggcggcgggc aaaagcaacc
     3961 gggagatagc tcaggagctg ttcatcacga ccaagaccgc ttcggcgcac ctcagccggg
     4021 cataccgcaa gctcgatgtc acccggcgga accagctggc cgaggcgctg gtcagtaaga
     4081 cgcccgccgt cagcaggcac gaggccaccc ggaccgctta gagcgccgat ttcctaaggc
     4141 gcaggcggca aaagacaggg atggcgcgcc cgatgccgcc
//
LOCUS       DATNBV010000404         4398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3057555,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000404 DATNBV010000000
VERSION     DATNBV010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3057555"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VN627_09625"
     CDS             <1..266
                     /locus_tag="VN627_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXD03554.1"
                     /translation="RGEGESIAVSIADNGIGLPQDRERITEPYMTTREKGTGLGLAIV
                     KKIVEEHGGEMTFAAAEGGGTMVTLNFARDPLAKGQRAPEAAE"
     gene            280..1668
                     /locus_tag="VN627_09630"
     CDS             280..1668
                     /locus_tag="VN627_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXD03555.1"
                     /translation="MALEILIVDDERDIRELVAGVLEDEGYECRTAGDSTSALEAVDQ
                     RRPSLVLLDVWLHGSPMDGLEVLDAIKAREPELPVIIFSGHGNIDTAVSAISRGAVDF
                     IEKPFEAERLLHLVGRATETEQLRRQNAQLRQHVVSDSFEFTGNSTAINQVRATLKRV
                     ANTGSRLLISGPAGVGKEVAARLLHSWSGRADHAFVSVNSARITPERFEHELFGEEQD
                     GNLIRPGLLEMADGGTLYLDEVADMPLSTQARILRVLTEQAFVRVGGVRQIRVDVRVV
                     SSTSRDLEREIEERRFREDLFYRLNVVPVQVPALSDRREDIPALVDQFFARFATDQGI
                     VPPEISPEAMAALQAYDWPGNVRQLRNVVERTVILTPREKLARIEADMLPDEISGGQL
                     GGDTAVSALMGVPLREAREHFEREYLKVQIRRFSGNISRTASFIGMERSALHRKLKLL
                     GMADRGDEPAEA"
     gene            1864..2436
                     /gene="hfq"
                     /locus_tag="VN627_09635"
     CDS             1864..2436
                     /gene="hfq"
                     /locus_tag="VN627_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HXD03556.1"
                     /translation="MTGRTLSARPRPPKPEPAPPPAPLTGKGNQNLQDQFLNLLRKNK
                     VPVTMFLVKGVKLQGIVTWFDNFSILLRRDGQSQLVYKHAVSTIMPSQPIDARVFSTG
                     ADTANKKVRLLQDVFLSSIRAAGVQVTMFLVNGVMLQGRVAAYDLFCMLLEREGYVQL
                     AYKHAVSTIQPVTPVDLTGGEFDDEEEDED"
     gene            2433..3773
                     /gene="hflX"
                     /locus_tag="VN627_09640"
     CDS             2433..3773
                     /gene="hflX"
                     /locus_tag="VN627_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXD03557.1"
                     /translation="MTDSEDLKGEVSRGARAVIVYPAMRQRASAGDIDSRLEEAKGLA
                     LAIGIVVTEAFAVPIREARAGTLFGEGQIANIAVACNREEAELVIVDGSLTAIQQRNL
                     EEKLKRKVIDRTGLILEIFGERAATAEGRLQVELAHLDYQAGRLVRSWTHLERQRGGF
                     GFLGGPGETQIEADRRMIRDRMARLRRELEQVRRTRGLHRQRRGRAPWPVIALVGYTN
                     AGKSTLFNRLTGASVMAEDLLFATLDPTMRAIRLPGIDKAILSDTVGFISDLPTQLVA
                     AFRATLEEVTAADVIVHVRDIANPDTSAQKKQVLEVLADLGVLPETQTGEGEPSIPLV
                     EVWNKADLLSAERIADLAEEAERRADERIVTLSALTGEGCDGLLALLSDLLTSDARPH
                     VFVIPAAAGQQIAWLHANGEVTGDEDAGEGEGGPLRRLTVRLTPKELGRFSRLS"
     gene            complement(3837..>4398)
                     /gene="era"
                     /locus_tag="VN627_09645"
     CDS             complement(3837..>4398)
                     /gene="era"
                     /locus_tag="VN627_09645"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HXD03558.1"
                     /translation="RPEPKILILNKVDVADKPKLLTHAAKLNEALAFDETFFVSAQTG
                     DGVPELKARLAALMPEGPWHYPEDQVSDASERLLAAEITREQLYRQLHDELPYDSAVR
                     PESYTQRKDGSVEIHQQIVIARDSQKGIVLGKRGAKLKAIGEAARKELAELLGCKVHL
                     FLHVRVEENWAESKEIYEEIGLDWVR"
BASE COUNT          737 a         1364 c         1523 g          774 t
ORIGIN      
        1 tgcgcggcga gggggagagc atcgcggtct ctatcgccga caacgggatc ggtctgccgc
       61 aggaccgcga gcggatcacc gagccctaca tgaccacgcg cgagaagggc acgggtctcg
      121 gcctcgccat cgtcaagaag atcgtcgagg aacatggcgg cgaaatgacc ttcgctgcgg
      181 ccgagggcgg cggcacgatg gttactctca attttgcgcg cgatcccctc gccaagggcc
      241 agcgcgcgcc cgaagcagcc gaatgagaaa ggtgacccga tggcgctcga aatcctgatc
      301 gtcgacgacg aacgcgacat tcgcgaactc gtggccggcg tgctcgagga cgaggggtac
      361 gagtgccgca cggccggcga cagcacttct gcgctcgaag ccgtggacca gcgccggcct
      421 agcctcgtcc tgctcgacgt ctggttgcat ggttcgccga tggacgggct ggaagtgctc
      481 gacgcgatca aggcgcgcga gcccgagctg cccgtcatca ttttctccgg ccacggcaat
      541 atcgatacgg cagtctccgc gatcagccgc ggcgcggtcg atttcattga aaaaccgttc
      601 gaggctgagc ggctgctgca ccttgtcggc cgcgctaccg aaaccgagca gctgcgccgt
      661 cagaacgccc agctgcgcca gcacgtggtg agcgacagct tcgaattcac cggcaacagc
      721 acggcgatca atcaggtccg cgcgactttg aagcgcgtcg ccaatacggg cagccggctg
      781 ctgatttcag ggcccgctgg cgtgggcaag gaagtggcgg ctcggttgct ccattcgtgg
      841 agcgggcggg ccgatcatgc gttcgtgtcg gtcaattcgg cgcggataac gcccgagcgg
      901 tttgaacacg agctgttcgg agaggagcag gatggcaatc tcatccgccc gggcctgctc
      961 gaaatggccg acggcggtac gctctacctc gacgaggtgg cggacatgcc tctttccacc
     1021 caggcccgta tcctgcgcgt gctgaccgag caggctttcg tccgcgtggg cggggtgcgc
     1081 cagatccgcg tggatgtgcg ggtcgtttcg tccacctcgc gcgatctcga gcgggagatc
     1141 gaggagcgcc ggttccggga agacctgttc taccggctga acgtggtgcc cgtccaggtt
     1201 cccgcccttt cggaccggcg cgaggacatt cccgcgctgg tcgatcagtt cttcgcccgg
     1261 ttcgccaccg atcaggggat cgtcccgccc gagatatcgc ccgaggcgat ggccgcgctc
     1321 caggcctacg attggccggg caatgtgcgc cagctgcgca acgtggtcga acgcacggtg
     1381 atcctcaccc cgcgcgaaaa gctggcgcgg atcgaggcgg acatgctgcc cgatgagatc
     1441 tcgggcggcc agctcggcgg cgacaccgcg gtgagcgcgc tgatgggtgt gccgctgcgc
     1501 gaggctcgcg agcatttcga gcgggagtac ctcaaggtcc agattcgccg gttttcgggc
     1561 aacatttccc ggacggccag cttcatcggg atggagcgct cggccctcca ccgcaagctc
     1621 aagctgctcg gcatggccga ccggggggat gagccggcgg aggcctgatg gccgttgcgc
     1681 gccgcgaata ttcctgaatt cgtctgcaca atcggaacct gcctccgctt tcccgattta
     1741 cggcgagagc ggaacccggt agtttgccct cgattgcggt tggaccggac ctttccatga
     1801 gggcctgccc agccagatcg cggaccgtga cacacggccg ccaagaaaaa ggagcaaata
     1861 ccggtgaccg gacgcactct ctccgcccgc cctcggcccc ccaagccgga gccggccccg
     1921 ccgccagccc cgctgacggg caagggcaac cagaacctcc aggaccagtt cctcaacctg
     1981 ctgcgcaaga acaaggtgcc tgtcaccatg ttcctcgtga agggcgtgaa gctgcagggg
     2041 atcgttacct ggtttgacaa cttctcgatc ctcctgcgcc gcgacgggca gtcgcagctg
     2101 gtctacaagc atgccgtctc gaccatcatg ccgagccagc cgatcgatgc gcgggtattc
     2161 tccaccggcg ccgacaccgc gaacaagaag gtgcggctgc tgcaggacgt gttcctctcg
     2221 tccattcgtg cggccggggt gcaggttacg atgttcctgg tgaatggcgt gatgctgcag
     2281 gggcgggttg ccgcctatga cctgttctgc atgctgctcg aacgagaagg ctatgtgcag
     2341 ctggcctaca agcacgcggt ctcgaccatc cagccggtca ctccggtaga tctgacgggc
     2401 ggcgagttcg atgacgagga agaggacgaa gactgaccga ttctgaagac ctcaagggcg
     2461 aggtcagccg tggggcgcgc gccgtcatcg tctatccggc gatgcggcag cgggcctcgg
     2521 cgggtgatat tgattcccgg ctggaggagg cgaagggcct ggcgctggcc atcggcatcg
     2581 tcgtcaccga agcctttgcc gtgcccatcc gcgaggcgcg ggccggcacg ctgttcgggg
     2641 aggggcagat cgcgaatatc gcggtggcct gcaaccgcga ggaggccgaa ctggtcattg
     2701 tcgacggctc tctcaccgcg atccagcagc gcaaccttga ggaaaagctc aagcgcaagg
     2761 tgatcgaccg caccggcctg atcctggaga tctttggcga acgggcggcg acggcggaag
     2821 gccggctcca ggttgaactg gcccaccttg attaccaggc ggggcgcctc gttcgctcgt
     2881 ggacccacct cgaacgtcag cgcggcggct tcgggttcct cggcgggccg ggcgagacgc
     2941 agatcgaggc cgaccggcgg atgatccgcg atcgcatggc ccgcctgcgc cgcgagctgg
     3001 aacaggtacg ccgcacccgc ggcctccatc gccagcgccg gggccgcgca ccgtggccgg
     3061 tcatcgcgct ggtcggctat accaatgccg gcaagtcgac gctgttcaac cggctgaccg
     3121 gggcttcggt catggccgag gacctgctgt tcgccacgct cgatccgacc atgcgggcga
     3181 tccgcctgcc cgggatcgac aaagcgatcc tttcggacac cgtcggcttc atctccgacc
     3241 tgccgacgca gctcgtcgct gcgttccggg cgacgttgga ggaggttacg gcggcggatg
     3301 tgatcgtcca tgtccgcgac atcgcgaacc cggatacatc ggcgcagaag aagcaggtgc
     3361 tcgaggtgct ggccgatctc ggcgtgttgc ccgagacgca gaccggggag ggggagccgt
     3421 cgatcccgct ggtcgaggtg tggaacaagg ccgacctgct gagcgccgag cggatcgccg
     3481 atctggcgga agaggcggag cggcgggcgg atgagcggat cgttacgctc tcggcgctga
     3541 cgggggaggg gtgcgacggg ttgctggcgc tgctctcgga cctgctgacc tcggatgcga
     3601 ggccgcatgt gttcgtgatc ccggcggcgg cggggcagca gattgcctgg ctgcatgcca
     3661 atggcgaggt gactggggat gaggatgcag gtgagggcga gggtggcccg ctgcggcggc
     3721 tgacggttcg gttgaccccc aaggagctgg ggcggttctc gcggcttagt tgatgaaata
     3781 cgtgcggatg agttgattga attttgtcta ataaaccatt ctaaatacac gatttatcag
     3841 cgaacccagt ccagtccgat ctcttcgtag atttctttgg actcggccca gttctcttcc
     3901 acgcggacgt ggaggaacag gtggaccttg cagccgagca gttcggccag ttccttgcgg
     3961 gccgcctcgc cgatggcttt cagcttggcg ccgcgcttgc cgaggacgat gcccttctgg
     4021 ctgtcgcgcg cgatgacgat ctgctggtga atctcgaccg agccgtcctt gcgctgggtg
     4081 tagctttccg gccggaccgc gctgtcatag ggcagttcgt cgtggagctg gcggtagagc
     4141 tgttcgcggg tgatctccgc ggcgagcagg cgctcgctgg catcggaaac ctggtcttcg
     4201 ggatagtgcc aggggccctc gggcatgagc gcggcgaggc gggccttgag ttcgggcacg
     4261 ccgtcgccgg tctgggcgga gacaaagaag gtctcatcga acgccagcgc ctcgttgagc
     4321 ttggctgcgt gggtgagcaa tttgggcttg tcggccacat cgaccttgtt gaggatcagg
     4381 atcttgggct cggggcgg
//
LOCUS       DATNBV010000405         5345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2790500,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000405 DATNBV010000000
VERSION     DATNBV010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2790500"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /gene="phaR"
                     /locus_tag="VN627_09650"
     CDS             complement(<1..529)
                     /gene="phaR"
                     /locus_tag="VN627_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HXD03559.1"
                     /translation="MAGKAGEEGDVVIIKKYANRRLYNTRSSSYITLDHLAKMTREGI
                     DFKVVDAKTGADITHQILTQIIMEEEANTGEQILPVNFLRQLIAMYGNSMQSLLPHYL
                     EASMDHFRDNQLKLRKALEESIGSNPLAAIAQRNMEMFKAAAAAFMPGMEVPGSERPA
                     EKPAETPPPSGGDDLA"
     gene            complement(645..1691)
                     /gene="alr"
                     /locus_tag="VN627_09655"
     CDS             complement(645..1691)
                     /gene="alr"
                     /locus_tag="VN627_09655"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXD03560.1"
                     /translation="MTAAFPAPPLRLACDTGALAANWRALDGLSGAARAGAAVKADAY
                     GTGARRAVPVLRDAGCRDFFVAHWCEAADLLDLVAPESLAMLHGPLTAEEAQWARASG
                     VRPVLNSLDQVDRWLAGGGGTAHVMVDTGMNRLGLPLEALGDARLARLEIDLLLSHLA
                     SAEEDSPLNAAQQERWLGARQRLPHGRASLANSAGIMLGPDYHGDVTRPGLSLYGGVP
                     CPALAPHIRQVVTPEAAILQVREIAAGETVGYNATFTAAAPMRIGVIALGYADGYLRC
                     WSDRGVARGGNGAALPVLGRVSMDMTAIDLGPAPALREGDWVGIDYALPDAAAQTGLS
                     QYELLTSLGARFVR"
     gene            complement(1702..3336)
                     /locus_tag="VN627_09660"
     CDS             complement(1702..3336)
                     /locus_tag="VN627_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD03561.1"
                     /translation="MNASIAQPPGAASAHAEPSASDMRLVIAASSAGTVFEWYDFFIY
                     GTLGAILSKTFFPTGNATLEMLLFWLVFAVGFGFRPLGAVLFGFLGDRLGRKYTFLVT
                     VVLMGVATAGVGMIPSAATIGWWAPGLVIVLRVLQGLALGGEYGGAAVYVAEHAPFPR
                     RGYFTSYIQAGVVGGFVLSLLVVLACKAAMTPETWLAWGWRVPFLLSFALLAISLWMR
                     LKLSESPVFLEMQEEGEIAGNPFVESFTYPGNSRRILVALFGVAAGLTVIFYTAMFTG
                     MGFLKSTMHMDDTAAEMIAGVAALVGIAFFVGAGHLSDRIGRKSPIVIGYALTLLLLF
                     PAFWTMGASGERAGGSAAWTIAGPDCRYDAFAKVQATDCGKLMGDFTGAGIGYTLTSA
                     PVLSLAAGNRAIDLAGLDWADAKARKAAIEERAGAKVFSAAKVKPGLRQVLLAGGAML
                     MLMALSGMTYGPVAALLSEMFPAKVRYSSMSIPYHIGTGYFGGFQPLIATYVVAKTGD
                     PYSGLWYTWGVVAMAFVVAIWGLKGGPPRDYGDDAN"
     gene            complement(3413..3835)
                     /locus_tag="VN627_09665"
     CDS             complement(3413..3835)
                     /locus_tag="VN627_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HXD03562.1"
                     /translation="MASAARLYTPEVLALATSLAAHPFDPAMPFAGSARSASCGSTLA
                     ISLALDGAGRVTGLGLRPQACAVGQASAAIFAGAVPGRTRSEIAAAEAQMAAWLGGAG
                     QMPDWPGLAAIAAARDYPARHGAILLPWRAALDALPPG"
     gene            complement(3838..4377)
                     /locus_tag="VN627_09670"
     CDS             complement(3838..4377)
                     /locus_tag="VN627_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HXD03563.1"
                     /translation="MTGFDIGVLVLVAMGAVTGLMRGFVQEVLTLLAWVISLVAIHYA
                     HSSVTHMLEGYMGNHFGAAGVLAFALLLLIPYAVVRLMANHLGRASRSSLLGPVDRVI
                     GMGFGAVKGMLIAVVGFSILVLGYDAAWGVDGRPDWITQSRTYPFINASSDGLVKMLG
                     ERRAEAAAAERKRLRGKAD"
     gene            complement(4469..>5345)
                     /locus_tag="VN627_09675"
     CDS             complement(4469..>5345)
                     /locus_tag="VN627_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HXD03564.1"
                     /translation="PPALLIIDSIQTMHSDQIEGAPGTVSQVRASAFELIRYAKDNGV
                     ALVLVGHVTKDGTIAGPRVLEHMVDVVMSFEGERSHQYRILRALKNRFGAVDEIGVFA
                     MDGQGLVEVPNPSMLFLSGRDEPIAGSAVFPALEGTRPVLVEIQALIVRLQSGATPRR
                     AVVGWDSGRLAMLLAVLEARCGLNFSSAEVYLNVAGGYRLADPAADLAVAAALVSGLA
                     DKPLPSGSTWFGEVSLAGEVRPVAHAGIRLREAGKLGFTDAYAPIESGSDVPGVRRHA
                     VSMLSRLVDRVMGET"
BASE COUNT          939 a         1908 c         1727 g          771 t
ORIGIN      
        1 tggcgagatc gtccccaccg ctcggcggcg gcgtctccgc cggcttttcc gccgggcgtt
       61 ccgaccccgg aacttccatg ccgggcatga aggcggctgc ggcggccttg aacatttcca
      121 tgttgcgctg ggcgatggcg gcaagcggat tgctgccgat gctctcttcc agcgccttgc
      181 gcagcttcag ctggttgtcg cggaaatggt ccatcgaggc ttcgagatag tgcggcagga
      241 gcgactgcat cgaattgcca tacatcgcga tcagctggcg caggaagttg accggcagga
      301 tctgctcgcc ggtattggcc tcttcctcca tgatgatctg ggtcaggatc tggtgggtaa
      361 tgtcggcccc ggtcttggcg tcgacgacct tgaagtcgat cccttcgcgg gtcatcttgg
      421 cgagatgatc gagcgtgatg tagcttgacg accgcgtgtt gtagagccgc cggttggcgt
      481 acttcttgat gatgacgacg tcgccctctt cgcccgcttt tcctgccatt ttacaaccct
      541 gcttcccgga tgcccgactc ggcacaatcc ggtcagctta ccccgaacgg tgtgcaacgc
      601 aacaagagcc gtgctgcgca gcacaagcgc ggcatcccgc ggccctagcg gacgaaccgc
      661 gcgcccagcg aagtcagcag ctcatattgc gaaaggccgg tctgcgccgc agcatcgggc
      721 agggcatagt cgatgcccac ccaatcaccc tcgcgcagcg cgggcgcggg gccaaggtcg
      781 attgccgtca tgtccattga aactcggccc agcaccggca aggccgcgcc gttgccgccg
      841 cgcgccacgc cccggtccga ccagcaacgc agataaccat cggcatagcc cagcgcgatc
      901 accccgatcc gcatcggcgc ggcggcagtg aacgtggcgt tatagcccac cgtctccccg
      961 gccgcgatct cgcgcacctg caggatcgcc gcctccggcg taacaacctg ccggatatgc
     1021 ggcgccagcg ccggacacgg cacgccgccg tagagcgaca ggccgggccg cgtcacatcg
     1081 ccgtgatagt ccgggcccag catgattccg gcgctgttgg ccaggctcgc ccggccatgg
     1141 ggcaggcgct gccgggcgcc cagccaccgc tcttgctgcg cggcattgag cgggctgtcc
     1201 tcctcggccg aggcgaggtg cgaaagcagc aggtcgatct ccagccgcgc cagcctcgca
     1261 tcgccgagcg cctcgagcgg caggcccagc cggttcatcc ccgtgtccac catcacgtgg
     1321 gcagtcccgc ctccgccagc gagccagcgg tcaacctgat ccagcgaatt gagcaccggc
     1381 ctcaccccgc tcgcccgcgc ccattgcgct tcttccgcgg tcagggggcc gtgcagcatg
     1441 gcgaggctct ccggcgcaac caggtcgagc aggtcagccg cttcgcacca gtgcgccacg
     1501 aagaagtcgc ggcacccggc atcgcgcagc accggcacgg cccggcgcgc acccgtgccg
     1561 taggcgtcgg ccttgaccgc ggccccggct cgcgccgcgc ccgacaagcc gtccagcgcg
     1621 cgccagttgg cggccagcgc accggtatcg caggcgaggc gcagcggcgg cgccggaaag
     1681 gccgcggtca tgacggagag atcagttggc atcgtcacca tagtcacgcg gcggtccgcc
     1741 cttgagcccc cagatcgcca ccacgaacgc catggcgacc actccccagg tgtaccacag
     1801 gcccgaatag ggatcgccgg tcttggcgac gacgtaggtg gcgatcagcg gctggaaccc
     1861 gccgaaatag cccgtgccga tatggtaggg gatcgacatc gatgaatagc gcaccttggc
     1921 gggaaacatt tccgacagca gcgccgcgac cggaccataa gtcatgccgg acagggccat
     1981 cagcatgagc atggcgccac ccgccagcag gacctgccgc aagcccggct ttaccttggc
     2041 cgcagagaac accttggccc cggcccgctc ctcgatggcg gccttgcgcg ccttggcatc
     2101 ggcccagtcc agccccgcga ggtcgatcgc ccggttgccg gcagcgaggg agagcaccgg
     2161 ggcactggtc agggtgtagc cgatgccggc accggtgaag tcgcccatca gcttgccgca
     2221 gtcggtcgcc tggaccttgg cgaaggcgtc gtagcggcaa tcgggcccgg cgatcgtcca
     2281 ggccgcgctg ccgcccgcgc gttcgcccga ggcgcccatc gtccagaacg cgggaaacag
     2341 cagcagcagg gtcagcgcat agccaatgac gatcggtgac ttgcgtccga tccggtcgga
     2401 caaatggcct gcgccgacga agaacgcgat gccgaccagc gcggccacgc cggcgatcat
     2461 ttccgctgcc gtatcgtcca tgtgcatggt cgacttgagg aagcccatgc cggtaaacat
     2521 cgccgtatag aagatcacgg tcagtccggc ggcgacgccg aacagggcca caagaatgcg
     2581 gcggctgttg cccgggtagg taaagctttc aacgaacggg ttgccggcga tttcaccctc
     2641 ttcctgcatc tccaggaaca ccgggctttc cgacagcttg agccgcatcc acagcgagat
     2701 cgccagtagc gcgaagctca gcaggaacgg cacgcgccag ccccaggcca gccaggtctc
     2761 cggcgtcatc gccgccttgc aagcaagcac taccagcagg ctcagcacaa agccgccaac
     2821 cacaccggcc tggatgtagc tggtgaaata gccgcgccgc gggaaaggcg catgttcggc
     2881 cacgtagacc gccgccccgc cgtattcgcc gccgagagcc agcccttgca aaacgcgcag
     2941 cacgattacg aggcccggcg cccaccagcc aatcgtcgcg gccgaaggga tcatcccgac
     3001 ccccgccgtg gctacgccca tcagcacgac tgtcacgagg aaggtgtact tgcgaccgag
     3061 ccggtcgccc aggaaaccga acagcacggc cccaagcggg cggaaaccga agccgaccgc
     3121 gaagacgagc cagaacagca gcatctcgag cgtggcattg ccggtcggga agaacgtctt
     3181 cgacaggatc gcgcccagcg tcccgtagat gaagaaatcg taccattcga acaccgtgcc
     3241 cgccgaggat gcggcgatga cgagacgcat gtcgctcgcg ctcggctccg catgggcgga
     3301 tgccgcaccg ggtggctggg caatgctggc gttcatctgg cggtctcccc cctttgcgtc
     3361 cggtcggcgc gcgtccggtc ctcgcgtcag ggctcaggct gtagccgggg attcaaccgg
     3421 gtggaagggc gtcgagggcg gccctccacg gcagcaggat cgccccgtgc cgcgccggat
     3481 aatcgcgcgc ggcggcaatc gcggcaagtc cgggccagtc gggcatctgc ccagctccgc
     3541 cgagccaggc tgccatctgc gcctctgccg cggcgatctc ggaacgggtg cgcccaggca
     3601 ccgcaccggc aaagatcgcc gcgctcgcct gccccaccgc acaggcctgc gggcgcagcc
     3661 cgaggccagt cacccggccg gctccgtcaa gcgccagcga gatcgccagc gtggaaccgc
     3721 aacttgccga acgggcgctt cccgcgaacg gcatggccgg gtcgaacggg tgtgccgcca
     3781 gcgatgtcgc cagcgccagc acctcgggcg tatagagccg cgcggcagag gccatcgtca
     3841 gtcggctttt ccacgcagcc gcttgcgttc cgccgctgcc gcctcggccc ggcgctcgcc
     3901 gagcattttc accaggccgt cgctgctggc attgatgaag ggataagtgc gcgattgggt
     3961 gatccagtcg ggccggccat ccacccccca ggcggcatcg tagccaagca ccaggatcga
     4021 gaagccgacg accgcgatca gcatgccttt taccgcgccg aagcccatgc cgatcacccg
     4081 gtccaccggt cccagcaacg aggagcggct ggcgcggcca aggtggttcg ccatcaggcg
     4141 gaccacggca taggggatca gcagcagcag ggcgaaggcc agcaccccgg ccgcgccgaa
     4201 gtggttgccc atgtagcctt cgagcatgtg cgtgaccgac gagtgggcgt aatggatggc
     4261 caccagcgaa atgacccagg ccagcagggt aaggacttcc tgcacgaaac cgcgcatcag
     4321 cccggtcacc gcccccatgg cgaccagcac cagaactccg atatcaaatc ccgtcatctg
     4381 gcgcggatcc cgtagtggcc ccaacgagcc gtgcggccct gccgttctca ggaccagtgg
     4441 tgctgaatcc ggcctgaatg ggcgtggtct aggtctcgcc catgacgcgg tcaacgaggc
     4501 gcgagagcat cgatacggcg tgtcgccgca cgccgggcac gtccgaaccg ctttcgatgg
     4561 gcgcataggc gtcagtaaag ccgagcttgc ccgcctcgcg caggcggatg ccggcgtggg
     4621 ccacgggccg cacctcgccc gccagcgaaa cctcgccgaa ccaggtcgag ccagatggca
     4681 gcggcttgtc cgccagcccc gaaaccagcg cggcggccac ggccaggtcc gccgccggat
     4741 ccgcgaggcg atagccgccc gcaacattta ggtagacctc cgccgaggag aagttgagcc
     4801 cgcagcgggc ctccagcacg gccagcagca tggccagccg accgctatcc cagcccacca
     4861 ccgcccggcg cggcgtcgcc ccgctctgca gccgcacgat cagcgcctgg atttcgacca
     4921 gcaccggccg cgtgccttcg agcgcgggga acaccgcgct gccggcgatc ggctcgtcgc
     4981 ggccggagag gaacagcatc gacgggttcg gcacttcgac gagcccctgc ccgtccatcg
     5041 caaacacgcc gatctcgtcg accgcgccga agcggttctt gagcgcgcgc aggatgcggt
     5101 actggtggct gcgctcaccc tcgaagctca tcaccacgtc gaccatgtgc tcgagcacgc
     5161 gcgggccggc gatcgtgccg tccttggtca cgtgcccgac cagcaccagc gcgacgccgt
     5221 tgtccttggc atagcggatc agctcgaacg cgctcgcccg cacctggctg acggtgccgg
     5281 gcgcgccttc gatctggtcc gagtgcatgg tctggatcga atcgatgatc agcaacgcag
     5341 gcggc
//
LOCUS       DATNBV010000406         2282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4353917,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000406 DATNBV010000000
VERSION     DATNBV010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4353917"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..680)
                     /locus_tag="VN627_09680"
     CDS             complement(69..680)
                     /locus_tag="VN627_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXD03565.1"
                     /translation="METILIADDHPLFRQALALAVGAVVPAARVVEAGTLATAARAAN
                     EAEGLKLILLDLKMPGAVGYSGIALLHAECPEVPILVVSSAEGASAAQDARAFGAVGF
                     LSKDSDLGAIEAAIAGALGSPAPAASPADQPVDAVRREVAQLTPTQLKVLLAVLGGKL
                     NKQIAYDLGLAERTVKMHRAALFRALGVKAGADAIRLAIEAGY"
     gene            complement(701..>2282)
                     /gene="acs"
                     /locus_tag="VN627_09685"
     CDS             complement(701..>2282)
                     /gene="acs"
                     /locus_tag="VN627_09685"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HXD03566.1"
                     /translation="KSRGVARGDRVTLYLPMVPEAAVAMLACARIGAIHSIVFAGFSP
                     EALAGRIEDCDSRVVVTADEGLRGGKHVPLKANVDEALAHCPGVETVIVLRHTGADLP
                     MVAGRDMDWAEGVAAQPGDCEPEEMDAEDPLFILYTSGSTGKPKGVLHTTGGYSVWAS
                     LTHEAVFDYRPGQIYWCAADIGWVTGHSYVVYGPLMNGATTVMFEGVPNFPDPSRFWQ
                     VVDKFGVEIFYGAPTALRALMREGDDWVKRTGRQTLRLLGSVGEPINPEAWEWYHKVV
                     GDGRCPIVDTWWQTETGGVMISPLPGATALKPGSATRPMFGVRPLLLDNEGKVLEGAG
                     EGCLVIADSWPGQMRTVWGDHERFFQTYFTTFPGYYFTGDGCRVDEDGYHWITGRIDD
                     VINVSGHRMGTAEIESALVAHAKVAEAAVVGMPHAIKGQGIYAFVTCNAEVEPDEALR
                     KELVHWVRHEIGPIATPDVIQFAPALPKTRSGKIMRRILRKIGENDLSSLDDTSTLAD
                     PAVVDNLVANRPELAGAG"
BASE COUNT          359 a          813 c          757 g          353 t
ORIGIN      
        1 gccctccgat gcggcgagga taaccgcgcc cgcataacgt cgccagtgcg gcggcccgcg
       61 ccgtgcgatc agtagccggc ttcgatggcg aggcggatgg cgtcggcacc ggctttcacg
      121 cccagcgccc ggaagagcgc ggcgcggtgc atcttcaccg tccgttcggc gaggccgaga
      181 tcataggcga tctgcttgtt gagcttgccg ccgagcacgg cgagcaggac cttgagctgc
      241 gttggcgtca gctgggccac ctcgcggcgc accgcatcga ccggctgatc cgccggagag
      301 gcggcgggag ccggggagcc aagcgcgccg gctatcgcgg cctcgatcgc cccaagatcg
      361 gaatccttgc tcaggaagcc gaccgcaccg aaggcccgcg cgtcctgcgc cgcgctcgcc
      421 ccctcggcgc tggagaccac caggatcggc acttccgggc actcggcgtg aagcagcgcg
      481 atcccggaat aacccaccgc gcccggcatc ttgagatcga gcaggatcag cttcaacccc
      541 tcggcctcgt ttgctgcgcg cgcggcggtt gccagcgttc cggcctcgac tacccgcgcc
      601 gcaggcacca ccgcgcccac cgccaaggcg agggcctggc ggaacagcgg gtgatcgtcc
      661 gcaatcagga tcgtttcgat ggagcctctc cgcgccggtt ttatcccgcg ccggcaagct
      721 ccgggcggtt cgccaccaga ttatcgacca cggcgggatc ggcaagggtc gaggtgtcgt
      781 cgaggctcga gaggtcgttc tcgccgatct tgcgcaggat gcgccgcatg atcttgcccg
      841 agcgggtctt gggcagggcc ggggcaaact ggatcacatc gggcgtggcg atcggcccga
      901 tctcgtggcg cacccagtgg accagttcct tgcgcagcgc ctcgtcgggt tcaacctcgg
      961 cattgcaggt gacgaaggca tagatcccct ggcccttgat cgcatggggc atgccgacga
     1021 cggcggcctc ggccaccttg gcgtgggcga ccagggcgct ctcgatctcg gccgtgccca
     1081 tgcggtggcc cgaaacgttg atcacgtcgt cgatccggcc ggtgatccag tgatagccgt
     1141 cctcgtccac ccggcagccg tcgccggtga aatagtagcc ggggaatgtg gtgaaatagg
     1201 tctggaagaa cctctcgtga tcgccccaca cggttcgcat ctgcccgggc cagctgtcgg
     1261 cgatcacgag gcagccttcg cccgccccct ccagaacctt gccctcgttg tcgagcagca
     1321 ggggcctcac cccgaacatc ggccgcgtgg ctgagccggg cttgagcgcc gttgccccgg
     1381 ggagggggga gatcatcacc ccgccggtct cggtctgcca ccaggtatcg acgatcgggc
     1441 agcgcccatc gcccaccacc ttgtggtacc actcccaggc ttccgggttg atcggctcgc
     1501 ccaccgaacc gagcaggcgc aaggtctggc ggccggtccg cttcacccag tcgtcgccct
     1561 cgcgcatcag ggcgcgcagg gccgtggggg cgccatagaa gatctccacg ccgaacttgt
     1621 cgaccacctg ccagaaccgg ctcggatcgg ggaagttggg cacgccttcg aacatcaccg
     1681 tggtcgcgcc gttcatcagc gggccgtaga ccacgtagga atggccggtc acccagccga
     1741 tatccgccgc gcaccagtaa atctgcccgg ggcgatagtc gaacacggct tcgtgagtca
     1801 gcgaggccca gaccgaatag ccgccggtgg tgtggagcac gcccttgggc ttgcccgtgg
     1861 aaccggaggt gtagaggatg aacagcgggt cttccgcgtc catttcctca ggctcgcaat
     1921 cgccaggctg ggcggcgacg ccttccgccc agtccatgtc gcggcccgcc accatcggca
     1981 ggtcggcccc cgtgtggcgc aagacgatga ccgtctccac ccccgggcaa tgggccagcg
     2041 cctcgtccac attggccttg agcggcacgt gcttgccgcc gcgcaggccc tcgtcagcgg
     2101 tgacgacgac ccggctgtcg caatcctcga tccgcccggc cagcgcctcg ggcgagaagc
     2161 cggcaaagac gatcgaatgg atcgccccga tccgcgcgca ggccagcatg gcgacggccg
     2221 cctccggcac catcggcagg tacagcgtca ccctgtcgcc gcgcgccacg ccgcggctct
     2281 tg
//
LOCUS       DATNBV010000407         6856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1518198,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000407 DATNBV010000000
VERSION     DATNBV010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518198"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1573
                     /locus_tag="VN627_09690"
     CDS             191..1573
                     /locus_tag="VN627_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXD03567.1"
                     /translation="MIALPDNILSDEGLPRVLSWIERKLGGKVVSTSRQSRWRPMYFV
                     DVETADGIRELVVRGDRVDVPCVFPLEHEMLMQDQMHRHGIEVPAVHGWIDDPRCYVM
                     DRVPGIAGFDGASDDQRQTVMRDYMKMLADLHRLPVEPFKAAGAHHAAAGDNPAEIGA
                     RHFENVIYRGTKKRPDPFLEFVLGWLRRNPPKRPAREAVVTWDSGQFHQLDGQLVAMI
                     DVELAHIGDPMMDLAAFRMRDTVLNYGDIGALYRQYEEFSGEPVDLDAIKWHHLFFTL
                     TNALSFHTALADPTPESDYMTNLQWVNETNLFAIEALAEYLDVDLPRVALPEPETTQV
                     APQFDHLVRSLTRIEAGDATAQHQVRILFRLARHLRRWDEIGRAVEQQVFDDVAEVIG
                     RRPTTWQESEQMLEDFVLADDGTHDLALIHLFNRKLQRAQALNGPAGSAMARHNPIQR
                     FDGRRAAFSG"
     gene            1952..4696
                     /locus_tag="VN627_09695"
     CDS             1952..4696
                     /locus_tag="VN627_09695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HXD03568.1"
                     /translation="MPALTLPALHASHGGCWLNEGGQTRAVGKGEAIVAAADTPVVLL
                     NAPLVASRLGYPDLSGLDLLELFAFVHPARFVVPTPKGLAHALGLPEPEGDSAVPALL
                     LAAAGQLLDTCQSPDWAEREGAWSQLQTLARLRWAWATLLSGHIARPERAERWLFARL
                     PEWEEAPERPQPIQTTLDPEAVTARLAELTGSGAESRPMQQAFAREAAQAFSPRRREG
                     QPHLVLAQAGTGIGKTLGYLAPASLWAEASGGTVWVSTFTKALQRQLRQESRRAWPGA
                     RADGSQPVVVRKGRENYLCLLNLEDALQGGFGGRAAILAQLVARWAAYTQDGDLVGGD
                     LPGWLGTLFRQRGIAALTDRRGECVYAGCPHYRKCFIERAARASAQADLVIANHALVM
                     VNAARGRDAAQRPTRIVFDEGHHVFEAADSTFAAALTGSEAIELRRWVIGPERGSKGR
                     RRGLSARLADVASYDDAGAEAITVARTAAEGLPADGWLQRLAEGAPSGEIEGLLGGVR
                     ALVYARDESGGQEAGYGLETEAAQLPGEFIELAGRAQEALGGLRQPLMKLGSRLEAIM
                     TEPPDWLDGAGRARIEGARHSLTWRIDLIAAWEALLQRLGGPADPEFVDWLAVDRSDA
                     REFDIGLHRRWLDPMKPFARVVLEGAHGVMMTSATLTDRGNGGVVDWDAAVTRTGAGH
                     VETAAHLFEAESPFNYAGQAEVLIVTDVAKGDISALAGAYGRLIEAAGGGALGLFTAI
                     RRLRAVYGRIADRLARGGLPLYAQHVDPIDTGTLVDIFRDDPRSSLLGTDALRDGVDV
                     PGRSLRLVVMEQVPWPKPSILHRARRAANGGSSADDRLIRARLAQAFGRLIRSQDDHG
                     HFVVLSAAFPSRLLGAFPPGTPIRRVTLDEALQRVAAGVHGPSGESQPREGAGLADDA
                     "
     gene            4707..5255
                     /locus_tag="VN627_09700"
     CDS             4707..5255
                     /locus_tag="VN627_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXD03569.1"
                     /translation="MKLLGLFRHAKSDWHDATARDFDRPLNDRGRKGAAIMGRHIDSH
                     GIKWQRVISSPAVRAAQTIEQAAEAAGKTIPIEWDRRIYLASSVTLADLLREIAEPTS
                     RVLLIGHNPGLEDLIFDLVPDDGSSPLREQVEEKFPTATYAVLELDIAKWAELDDGCA
                     RLVHMMRPRDLDPALGPSLIED"
     gene            complement(5277..5753)
                     /locus_tag="VN627_09705"
     CDS             complement(5277..5753)
                     /locus_tag="VN627_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXD03570.1"
                     /translation="MPDSPAPSLFDQAPAGPVSGDGGAVPAWVSVASDATRGDGKAPD
                     ALRTIGEVAAALGIRQHVLRYWEEQFPMLRPLKRSGSRRYYRPEDIALVERIDQLVHR
                     EGYTLRGARLVLQGRGAEAAGEMVAVPEVAALAAGGSSATSERLKAIRSRLAAALQ"
     gene            complement(5774..6070)
                     /locus_tag="VN627_09710"
     CDS             complement(5774..6070)
                     /locus_tag="VN627_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HXD03571.1"
                     /translation="MRSMSTLTRADLADAINRRMGFSRAESLAMVESILSHMCDALAD
                     GENVKISGFGTFLLRDKAERIGRNPKTGVEVPITPRRVLTFRASQMLKDQISGL"
     gene            complement(6247..>6856)
                     /locus_tag="VN627_09715"
     CDS             complement(6247..>6856)
                     /locus_tag="VN627_09715"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HXD03572.1"
                     /translation="GVADSMLRSGMADRALVIGAETFSRILDWEDRGTCVLFGDGAGA
                     ILLEAREVEEATGPGILATRLHADGQHNELLYVDGGPSTTGTVGKLRMKGKEVFKHVV
                     TNLADVLRETLEAAGLPAERLDWVVPHQANKRILDGTARKLGLSPDRVVVTVDQHANT
                     SAASVPLALDVAVRDGRIKPGQLVMLEAMGGGFTWGASLIRI"
BASE COUNT         1098 a         2347 c         2320 g         1091 t
ORIGIN      
        1 agttcggcgg gcaattcggg gctggcgggc gcgttggtca tgatcgcgcg cgtttcgcgg
       61 atgcggcgca cttgtgcaag tgccaatccc gcccgcgcat cacccaccgg atgcccgggg
      121 cggcacgatt gacgtgatcg gcccggtggc ggaccctgcc cggcaatgaa ccagcggacg
      181 ggagagaccg atgatcgcgc tgcctgacaa catcctgtcc gacgaaggcc tgccccgggt
      241 gctcagctgg atcgagcgca agctgggcgg caaggtggta agcaccagcc gccagtcgcg
      301 ctggcggccg atgtacttcg tcgacgtgga gaccgctgac ggtatccgcg agctggtcgt
      361 acgcggagac agggtggacg tgccctgcgt gtttccgctc gaacacgaaa tgctgatgca
      421 ggaccagatg catcgccacg ggatcgaggt ccccgcggtt cacggctgga tcgacgatcc
      481 acgctgctac gtgatggacc gcgtgcccgg cattgccgga tttgacggcg ccagcgacga
      541 ccagcgccag acggtgatgc gcgactacat gaagatgctg gccgatctgc accggctgcc
      601 ggtggaaccg ttcaaggcgg cgggcgccca tcacgccgcc gctggcgaca acccggcaga
      661 gatcggggcg cggcacttcg agaacgtgat ctaccgcggg accaagaagc gccccgatcc
      721 gttcctcgaa ttcgtgctcg gctggctgcg gcgcaacccg cccaagcggc cggcgcgcga
      781 ggccgtggtc acctgggatt cgggccagtt ccaccagctg gacggccagc tggtggcgat
      841 gatcgacgtc gagctcgccc acattggcga tccgatgatg gatcttgcgg cattccgcat
      901 gcgcgacacc gtgctcaact atggcgatat cggagcgctg taccggcagt acgaggagtt
      961 ttcgggcgaa ccggtcgatc tcgacgcgat caagtggcac cacctgttct tcacgctgac
     1021 caatgccttg tcgttccaca ccgcacttgc cgatccgacc ccggaatcgg actacatgac
     1081 caatctccag tgggtgaacg agaccaacct gttcgccatc gaggcgctcg cggaatacct
     1141 cgatgtcgat ctgcccaggg ttgccttgcc ggagcccgaa accacccagg tcgcgccgca
     1201 attcgatcat ctggtgcgca gcctgacgcg gatcgaggcg ggcgacgcca cggcgcagca
     1261 tcaggtccgc atcctgttcc gcctcgcccg tcacctgcgc cgctgggacg agatcggccg
     1321 cgccgtggag cagcaggtgt tcgacgacgt cgccgaggtc atcggcaggc gccccaccac
     1381 ctggcaggag agcgagcaga tgctggagga cttcgtgctg gccgatgacg gcacccacga
     1441 tctcgctctc atccacctgt tcaaccgcaa gctccagcgc gcccaggcgc tcaacggacc
     1501 ggctggttcg gccatggccc ggcacaatcc catccagcgt ttcgatgggc gtcgggcggc
     1561 attctccggc tgatccaggc ctacgcatgc ggctcgtcgc aaggcgcggg aacccgcgca
     1621 atgcccgcgc attggcaagg attgaagggg gcacggacga cctgcccgga tcgcccgccc
     1681 ctcggttcgc caggggaact gcccatgagc ctttcacgca tgacgttgtc tggagccacg
     1741 atttcttgct cccgtcccga tcggtggatc gcaccgcatc agtcactcga tccgtgcatg
     1801 cggcggctga cctacggtcc ggtccggccg atggactgga gcgaacggcc attctggcgg
     1861 cgattgttcc accgggggta aacgaccttg gcgagcgcca tgaggctgcc cgcatgttta
     1921 cttttcgttc ctcccccggc atgaatcggt catgcctgcc ctcaccctcc ccgcgctcca
     1981 tgccagccat ggcggctgct ggctgaacga gggcgggcag acccgcgctg tcggcaaggg
     2041 cgaagccatc gtcgctgccg ctgatacgcc ggtcgtcctg ctcaacgccc cgctggtggc
     2101 gagccgcctc ggctatcccg atctttccgg gctcgacttg ctcgagctgt ttgccttcgt
     2161 ccaccccgcg cggtttgtcg tgcccacgcc caaggggctg gcccatgcgc tgggcctgcc
     2221 cgagccggag ggcgattcgg ccgtgccggc acttctgctg gcggcggcgg ggcagttgct
     2281 cgacacctgc caatcgcccg attgggccga gcgtgagggg gcgtggagcc agctgcaaac
     2341 gctcgcccgc ctgcgctggg cctgggccac gctgctttcc ggccatattg cacggcccga
     2401 gcgcgccgaa cgctggctgt tcgcgcgcct gccggaatgg gaggaagcgc ccgagcgccc
     2461 gcagccgatc cagaccacgc tcgatcccga ggcggtcacc gcgcgcctgg ccgaactgac
     2521 cggcagcggt gcggagagcc ggccgatgca gcaggccttc gctcgcgagg ccgcgcaggc
     2581 cttctccccg cgccggcgcg agggccagcc gcacctcgtt ctggcgcagg ccggcacggg
     2641 gatcggcaag acccttggct atctggcgcc tgcctcgctg tgggccgagg ccagcggtgg
     2701 gaccgtctgg gtttccactt tcaccaaggc gctccagcgg cagctgcgcc aggaaagccg
     2761 ccgcgcctgg cccggcgccc gggccgatgg cagccagccg gtagtcgtgc gcaaggggcg
     2821 cgaaaactac ctgtgcttgc tcaaccttga agatgcgctg cagggcggct ttggcgggcg
     2881 ggcggcgatc ctggcgcaac tggtggcgcg ctgggccgcc tacacccagg acggcgacct
     2941 cgtcggcggg gacctgcccg gctggctcgg caccctgttc cggcagcgcg ggatcgccgc
     3001 gctgaccgac cggcggggcg aatgcgtcta tgccggatgc ccgcattacc ggaagtgctt
     3061 catcgagcgc gccgcgcggg ccagcgccca ggccgacctt gtcatcgcca accatgcgct
     3121 ggtcatggtc aatgccgcgc gggggcgcga tgcggcgcag cggccaacgc ggatcgtgtt
     3181 cgacgagggc caccacgtgt tcgaggcggc cgattccacc tttgccgcgg cgctgaccgg
     3241 cagcgaagcc atcgagctac ggcgctgggt catcgggccc gagcgtggct ccaagggtcg
     3301 gcgccggggg ctttcggcgc ggcttgccga cgtggcttcc tatgacgatg ccggcgccga
     3361 ggcgatcacc gttgcccgca cggcggccga gggcctgcct gcggacggct ggctccaacg
     3421 gctggcggaa ggcgcacctt cgggcgagat cgagggcctg ctcggcggtg tccgcgccct
     3481 cgtctacgcg cgggacgaga gcggcggcca agaggccggc tatggcctcg aaaccgaggc
     3541 cgcgcagctg ccgggggaat tcatcgaact ggccggccgg gcgcaggagg ccctgggcgg
     3601 gctgcgccag ccgctgatga agctgggctc gcggctcgag gcgatcatga ccgagccgcc
     3661 tgattggctc gacggcgctg gccgggcccg gatcgaggga gcgcgccatt cgctgacctg
     3721 gcgaatcgac ctgatcgccg cctgggaggc cctgctccaa cggctgggcg gcccggcaga
     3781 cccggaattc gtcgattggc tggcggtcga ccgctcggac gcgcgcgagt tcgatatcgg
     3841 cctgcaccgg cgctggctcg atccgatgaa gccgttcgcg cgggtcgtgc tcgaaggggc
     3901 gcacggggtg atgatgacca gcgcaaccct caccgaccgg ggcaatggcg gggtggttga
     3961 ctgggatgcg gccgtgacgc gcaccggcgc gggacatgtc gagactgcgg cgcacctgtt
     4021 cgaagcggaa agcccgttca actatgccgg gcaggccgaa gtgctgatcg tcaccgatgt
     4081 cgccaagggc gatatctcgg cgctggccgg agcctacggc cggctgatcg aggcagcggg
     4141 cggcggggct ctggggctgt tcaccgccat ccggcggctg cgcgccgtct atggccggat
     4201 tgccgaccgg cttgcccggg gcggactccc gctttatgca cagcatgtcg atccgatcga
     4261 taccggcacg ctcgtcgata tcttccgcga cgatccgcgc tcaagcctgc ttggcaccga
     4321 tgccctgcgc gacggggtcg atgtgccggg ccgctcgctg cggctggtgg tgatggaaca
     4381 ggtgccctgg cccaagccct ccatccttca ccgggcccgg cgggcagcca acgggggcag
     4441 ctcggcggac gaccggctga tccgcgcccg tctcgcccag gccttcgggc gcctgatccg
     4501 cagccaggac gatcacggcc acttcgtcgt cctctccgcc gccttcccca gccgcctgct
     4561 cggcgccttc ccgccgggca cgccgatccg ccgcgtcacg ctcgacgagg ctttacaacg
     4621 ggtggccgcc ggggttcatg gcccaagcgg cgaatcccag ccgcgcgagg gagccgggct
     4681 ggcagacgac gcatgaaact tcgctcatga aacttctggg gctgttccgc cacgccaagt
     4741 cggactggca cgatgcgacc gcgcgcgatt tcgaccgccc gctcaacgac cggggtcgca
     4801 agggcgcggc gatcatgggg cgccatatcg acagccacgg gatcaagtgg cagcgggtga
     4861 tctcgagccc cgccgtgcgc gcagcgcaga ccatcgagca ggccgcggag gccgcgggca
     4921 agaccattcc gatagaatgg gaccggcgga tctatctcgc cagttcggtc accctcgccg
     4981 acctcctgcg cgaaatcgcc gagccgacca gccgggtact gctgatcggt cacaatcccg
     5041 ggctggaaga cctgatcttc gatcttgtgc cggatgacgg ctcgagcccc ttgcgcgaac
     5101 aggtggagga gaagttcccc accgcgacct atgccgtgct ggaacttgac atcgccaagt
     5161 gggcggagct cgacgatggc tgcgcccggc tggtccacat gatgcgcccg cgcgaccttg
     5221 acccggcgtt ggggccgagc ctgatcgagg attaacccga ctgagccgat gtagcgctat
     5281 tgcagcgccg ctgcgaggcg ggagcgaatg gctttcaaac gctcagatgt agccgaactg
     5341 ccgccggcgg cgagcgctgc gacctcggga accgcgacca tctcgccagc cgcttcggca
     5401 ccccggccct gcagcacgag cctggcgccg cgcaaggtat agccttcccg gtggaccagt
     5461 tggtcaatcc gctcaaccag cgcgatatct tccgggcgat aataccggcg cgaaccgctg
     5521 cgcttcaacg gacgcagcat cgggaactgc tcttcccaat aacgcagcac gtgctggcga
     5581 atgccgagcg ccgcggccac ctcgccgatc gtgcgcaggg catcgggtgc cttgccgtcg
     5641 ccccgcgtcg cgtctgacgc gaccgacacc caggccggca ccgccccgcc atcgccgctc
     5701 accggtcctg ctggggcctg gtcgaacagc gaaggggcgg gactatccgg catcggtgac
     5761 tatcctgcgg ggctcaaagc cctgaaatct ggtccttcag catctggctc gcgcggaagg
     5821 tgagcacgcg gcggggcgtg atcggcacct cgaccccggt cttcggattg cggccgatcc
     5881 gctctgcctt gtcgcgcagg aggaaggtac cgaacccgga aatctttacg ttttcgccat
     5941 cggccagggc atcgcacatg tggctcagga tggattccac catcgccagc gattcagcgc
     6001 gcgaaaagcc catgcgacgg ttgatcgcat cggccagatc agcccgggta agcgtactca
     6061 tggaacgcat tgaaacccct tccccctaaa gtatccacga cccctcgaat cccacggcgg
     6121 aatttagcgg cagcacggaa aaaggcaacg caatcgcttg tttggcactg aaaatcggtg
     6181 cgaaaagcgg agcttgcgtc ctgccggcgt tcgaaaccat aaccttagtt atggaatttg
     6241 gcccggtcag atgcggatca ggctggcgcc ccaggtgaag ccgccgccca tggcttcgag
     6301 catgaccagc tggcccggct tgattcgacc gtcacgcacg gcgacatcaa gggccagcgg
     6361 aaccgaggcg gccgaggtat tggcgtgctg gtcgaccgtg accaccaccc ggtctggaga
     6421 gaggcccagc ttcctggccg tgccgtcgag aatgcgcttg ttggcctggt ggggcacgac
     6481 ccaatcgagg cgctccgccg gcaagccagc cgcctcgagg gtttcgcgca gcacatcggc
     6541 aaggttggtc accacgtgct tgaatacttc cttgcccttc atcctgagct tgccgaccgt
     6601 gccggtcgtg gaagggccgc catcgacata gagcaattcg ttgtgctggc catcggcatg
     6661 gaggcgcgtg gcgaggatgc cgggaccggt cgcctcttca acctcgcgcg cttcgagcag
     6721 gatcgccccg gcgccgtcgc caaagaggac gcaggtccca cggtcctccc agtcgagaat
     6781 gcggctgaag gtttcggcgc cgatcaccag cgcccgatcg gccatgcccg agcgcagcat
     6841 cgaatcggcc acgccg
//
LOCUS       DATNBV010000408         7483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4069655,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000408 DATNBV010000000
VERSION     DATNBV010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4069655"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VN627_09720"
     CDS             <1..621
                     /locus_tag="VN627_09720"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HXD03573.1"
                     /translation="ARIPAPERADAALQVPTTPERQAIVERLVEKSRYYEEARKLGHI
                     AASQAIVEDGMRQFVVCSGGGPSIMEAANRGAVEAGGETIGLNIVLPHEQAPNSWVTP
                     GLSFQFHYFALRKMHFLLRARAVAVFPGGFGTFDEFFELLCLIQTCKMKPIPILLFGG
                     DFWKRVVNFEALAEEGVINREDLNLFTPVETAEEGWDVIRRFYDLD"
     gene            complement(606..1367)
                     /gene="thiD"
                     /locus_tag="VN627_09725"
     CDS             complement(606..1367)
                     /gene="thiD"
                     /locus_tag="VN627_09725"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HXD03574.1"
                     /translation="MTTPPRILVIAGSDSSGGAGIQADIKTITMLGGYAMTAITTITA
                     QNTLGVTALEALSPELIAAQIDACTSDIGVDAVKIGMLGSAEIGHVVADRLARLNCPI
                     VFDPVMIATSGAVLADAETIAAFERLMRLATLTTPNVPELAALGGEAGMAARGVTYLA
                     KGGDAQGAVVEDRLVVPGSDPVVWRAERIPTRHTHGTGCTLASAITTGLGGGLDLASA
                     VEHGRAFVRAALLAAPGFGGGHGPLGHQAVVQSRS"
     gene            complement(1364..1588)
                     /locus_tag="VN627_09730"
     CDS             complement(1364..1588)
                     /locus_tag="VN627_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HXD03575.1"
                     /translation="MLEMRPDCERCGTDLPADLPGAFICSFECTFCAECADALDEACP
                     NCGGELLDRPTRARRLLADNPASTVRHFRG"
     gene            complement(1856..3196)
                     /gene="glmM"
                     /locus_tag="VN627_09735"
     CDS             complement(1856..3196)
                     /gene="glmM"
                     /locus_tag="VN627_09735"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HXD03576.1"
                     /translation="MGKQYFGTDGIRGRTNAGAMTAATAMKVGQAAGTRFLRGDHRHR
                     VVIGKDTRLSGYMLENAMVAGFTSVGMDVVLLGPMPTPAVALLTRELRADVGVMISAS
                     HNPYEDNGIKLFGPDGFKLSDEDELAIEAMLGQDIPLADSPEIGRARRIDDARGRYIH
                     AVKASLPENVRLDGLKIVVDCANGAAYQVAPSAIWELGAQIITVGVSPNGKNINDKCG
                     STHLDLVRETVVASGADIGIALDGDADRLIVIDEQGAVVDGDQIMALLGTQMAARGAL
                     RGGGVVATVMSNLGLERCLAGHGLTLERTAVGDRHVLERMRSGGFNVGGEQSGHMILL
                     DHATTGDGTVAALQVLAALVQSGKKASELLHLFDPVPQLLKNVRFAGGKPLENARVKA
                     VIADAEKQLNGRGRLVIRPSGTEPVIRVMAEGDDAREVEQVVDAICDVVREAAA"
     gene            3392..4003
                     /locus_tag="VN627_09740"
     CDS             3392..4003
                     /locus_tag="VN627_09740"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HXD03577.1"
                     /translation="MSISLMPLPYARDALAPAISADTLDTHHGKHHKAYVDKTNAAIA
                     DGPLADASLEEIVARSRGSNQGLFNNAAQTWNHGFYWASLAPAPSRPQGDLAAAIERS
                     FGGLDQLTQELATRGANHFASGWVWLASNNGALSVEETHDAETLADAGPVPLLVIDLW
                     EHAYYLDRKNVRPDYLEAVLDHVLNWDFAAENLARGRRWTYPA"
     gene            complement(3991..5262)
                     /locus_tag="VN627_09745"
     CDS             complement(3991..5262)
                     /locus_tag="VN627_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXD03578.1"
                     /translation="MTEAEILAKRTHSGPPPARQPFATAESEAVRSDPAIFAAQELRL
                     TSALVMLLGIGLFLALPFVLSIGSVVFLPLVTALMLSVVLSPLADQFLRTGLPNAIAS
                     MLAVLVLVLFLLVAITLIVQPALALVDDVPAMVRNATQQIVKLRSSFDWLNDISKALA
                     RVSGRPATREVVVATPSLIEQLAVATPSVILETLLTFLMAYFMVESRLRMRRHLLLDR
                     TSVGSSLKAARLLREIQDRVGTYILTVAMINVGVGAITALGAWLLGWNAPIMWGGIAA
                     LLNFVPYIGPLSMIGVLALVGLSTASSIGPALIAPAAYLCLHAIESNVVTPSILGRRF
                     TVNPVLILFSFSYFTWVWGVLGALLSVPILITLTATFEHLGKPNLIGFIFGEPLFPNL
                     PEVAAGEETTGGAAEEQVVPGASPAGPAQAG"
     gene            complement(5311..5961)
                     /locus_tag="VN627_09750"
     CDS             complement(5311..5961)
                     /locus_tag="VN627_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXD03579.1"
                     /translation="MSADPASDEPTQPGAPRVFTPRSEKDKRAFKRELTEVIPHLRAF
                     ARGLCGRPDLADDLVQETLLKAWAAQERFEPGTSMRAWTFVILRNAYLTDMRRNRFRG
                     EYDEAVAERTLTAPAGQEAPLHLSDMHRALLTLPPERREALLLVGAGGFSYEEAAEIC
                     QCAVGTIKSRVGRARAALTRMIESGDLPRRGHGEEDAAHAAIMKDLEHVSHGGSLK"
     gene            complement(5958..6131)
                     /locus_tag="VN627_09755"
     CDS             complement(5958..6131)
                     /locus_tag="VN627_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HXD03580.1"
                     /translation="MTSDKAKKPMRPATSRESKRARPEWADGLRQLYDSVLNEPLPDT
                     FADLISKLDESEK"
     gene            6414..>7483
                     /locus_tag="VN627_09760"
     CDS             6414..>7483
                     /locus_tag="VN627_09760"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HXD03581.1"
                     /translation="MGIFLLVSGITALSVYAIEQVETQRAEAQHRQMAGAASSAIERL
                     VNANAVYLRAGSLLTADSANASGAFSEFARQLDTDRSLRSGGALAWSAVVPSEEVPAL
                     EARMRAAGAASYTVHPRPVGGTVMPMVYVHPDSPDARKALGFDVLSEGERRTALDRAL
                     RSDKPVASGTIQLAISDLAGRRTGFGMFMPVYGSVQGHRRLVGVLSRGFAAQSFLEAA
                     LSGERLEDYGVALYDAESPGSWPMATMGEQSRRGERIVREMDLAGHRMRLEISPPAPS
                     TLSNLSLLTLLFGMMVAALLLILARLVTQQAAEDRAALAWFEEQASIRNSLTRELNHR
                     VKNTLANVLSIVALTRRRTDNI"
BASE COUNT         1198 a         2476 c         2590 g         1219 t
ORIGIN      
        1 gcccggattc ccgcgcccga gcgcgccgat gcggccctgc aggtgccgac cacgccggaa
       61 cggcaggcca tcgtcgagcg gctggtggaa aaatcgcgct actatgaaga ggcccgcaag
      121 ctcgggcaca tcgctgccag ccaggccatc gtcgaggacg ggatgcggca gttcgtggtc
      181 tgctcgggcg gcggtccttc gatcatggaa gcggccaatc gcggcgcagt ggaagcgggg
      241 ggagagacca tcgggctcaa catcgtcctg ccgcacgagc aggcgcccaa ttcgtgggtg
      301 acgccgggcc tgtcgttcca gttccactac ttcgccctgc gcaagatgca cttcctgctg
      361 cgggcgcggg cggtggccgt gttccccggc ggctttggca ccttcgacga gtttttcgag
      421 ctgctctgcc tgatccagac ctgcaagatg aagccgattc cgatcctgtt gttcggcggg
      481 gatttctgga agcgcgtggt caatttcgag gcgctcgccg aggaaggggt gatcaaccgt
      541 gaggacctca acctgttcac cccggtggaa acggcggaag agggctggga cgtgatccgc
      601 cggttctacg acctggactg aaccaccgct tgatggccca gcgggccatg gccgccgccg
      661 aacccggggg ccgcgagcag ggcggcccgg acgaaggcac gcccgtgttc gaccgcactc
      721 gccaggtcca gcccgccacc cagcccggtg gtgattgcgc ttgccagggt gcagccggtg
      781 ccatgggtgt ggcgcgtggg gatacgctcg gcccgccaga ccaccggatc gctgccgggc
      841 acgaccagac ggtcttccac cactgcgccc tgcgcatctc cgcccttggc gagataggtc
      901 accccgcgcg ccgccattcc ggcttccccg ccgagggcgg cgagttccgg cacgttgggc
      961 gtggtcagcg tggcgagccg catcaggcgc tcgaaggcgg cgattgtttc cgcgtcggcc
     1021 aggaccgccc cgctggtggc gatcatcacc ggatcgaata cgatggggca attcagccgc
     1081 gccagacggt cggcaacgac atgcccgatt tcggccgagc caagcatgcc gatcttcacc
     1141 gcatcgacgc cgatgtcgct cgtgcaggcg tcgatctgcg cggcgatcag ttcgggcgag
     1201 agcgcctcga gggcagtgac gccgagcgtg ttctgggcag tgatcgtggt gatcgcggtc
     1261 atcgcatagc cgccgagcat ggtgatggtc ttgatgtcgg cctggatgcc ggcaccgcct
     1321 gaggaatccg aaccggcgat gacgaggatg cgggggggcg tggtcatccc ctgaagtgtc
     1381 gcaccgtcga ggccgggttg tctgccagca gccgcctggc ccgcgtcggc cgatccagca
     1441 gttcgccgcc gcaattgggg caggcttcgt cgagtgcatc ggcgcattcc gcgcagaagg
     1501 tacactcgaa ggagcagatg aaagctcccg gcaggtcggc cggcaggtcg gtgccgcagc
     1561 gctcgcagtc ggggcgcatc tccagcatga gagcctttcc ttggcagttg gcaccgtcgt
     1621 gacggagcga aattggcccc agtgcaaggc gcgaggaggg agctatgctg ctgcatagtg
     1681 accgacgagc aacgccgcaa tggggccaat ttcgtccgcc gctgcgcggt cgcgtcagga
     1741 ggggcgcggg cggcgtcgcg atgcttgcag gggcaaccag cccctgccgc gcaccgctcc
     1801 tatcccgcgt cccttctgac gcgatcacga tggcgccaac tgccaaggaa aggctctagg
     1861 ccgcggcctc acgaaccacg tcgcagattg catcgaccac ctgctcgact tcgcgcgcgt
     1921 cgtcgccctc ggccatgacg cggatcaccg gctcggtccc cgaaggccgg atcaccagcc
     1981 gtccgcgccc gttcagttgc ttctcggcat cggcgatcac cgccttgacc cgcgcgttct
     2041 ccagcggctt gcccccggca aagcgcacgt tcttgagcag ctgtggcacc ggatcgaaca
     2101 ggtggagcag ttcgctcgcc ttcttgcctg actggaccag cgcggcgagg acctgcaggg
     2161 cggccacggt gccgtcgccg gtggtcgcgt ggtcgagcag gatcatgtgg ccggattgct
     2221 cgccgccgac gttgaagccg ccgctgcgca tccgttcgag cacgtggcgg tcgcccacgg
     2281 cggtgcgttc gagcgtcagg ccatggccgg cgaggcagcg ttcgaggccg aggttggaca
     2341 tgaccgtcgc caccacgccg ccgccgcgca gagcccctcg cgccgccatc tgcgtgccga
     2401 gcagggccat gatctggtcg ccatcgacga ccgcgccctg ctcgtcgatg acgatcagcc
     2461 ggtcggcatc gccgtcgagg gcaatgccga tgtcggcccc gctcgccacc accgtctcgc
     2521 gcaccaggtc gaggtgggtg gagccgcact tgtcgttgat gttcttgccg ttggggctga
     2581 cgccgaccgt gatgatctgg gcgcccagtt cccagattgc gctgggagca acctggtagg
     2641 cagcgccatt ggcgcagtcg accacgatct tgagcccgtc gagccggacg ttctccggca
     2701 gcgaggcctt gaccgcgtgg atgtagcggc cgcgggcatc gtcgatgcgc cgggcgcggc
     2761 cgatctcggg gctgtcggcc agtggaatgt cctggccgag catggcctcg atggcgagtt
     2821 cgtcctcgtc cgagagcttg aacccgtcgg ggccgaacag cttgatgccg ttgtcctcgt
     2881 aggggttgtg gctggccgag atcatcacgc cgacatcggc ccgcagttcg cgggtgagca
     2941 gggctactgc gggcgtgggc atgggcccga gcaggacgac gtccatgccg acgctggtga
     3001 agccggcgac catggcgttc tccagcatgt agccggagag gcgagtgtcc ttgccgatca
     3061 ccacccggtg acggtgatcg ccgcgcagga agcgggtgcc ggcggcctgg ccgaccttca
     3121 tcgccgtcgc cgcggtcatc gccccggcat tggttcgccc gcggatgccg tcggtgccga
     3181 aatactgctt gcccatgcct gttcgtcaac tcctgtgcgc ccgtgttcgc aatgccgcag
     3241 gcgggcgcga atgtcaccct gccagatggc tgcgacggac cgagtccatc atgcggccag
     3301 cggggcaacc ttcctttgga tagggcgttc cctgcgggag ccggcgcgcc gcgccgcggc
     3361 tctgccccat tcctgtcccc acggagattg catgtccatt tccctgatgc ccctgcccta
     3421 tgcccgcgac gccctggcgc ctgcgatctc cgccgatacg ctcgacaccc atcacggcaa
     3481 gcaccacaag gcctatgtcg acaagaccaa cgccgccatt gccgatggtc ccctggcaga
     3541 tgccagcctc gaggagatcg tcgcccggtc tcgcgggagc aaccagggcc tgttcaacaa
     3601 tgccgcgcag acctggaacc acgggttcta ctgggcgagc ctcgctccgg caccgtcccg
     3661 gccgcagggg gacctggccg ccgcgatcga gcggtcgttc ggcggtctgg accagctgac
     3721 gcaggagctg gccacccgcg gggccaatca tttcgcctcg ggctgggtct ggctggcgag
     3781 caacaatggc gcgctcagcg tggaagagac tcacgatgcc gagaccctcg ccgatgccgg
     3841 gccggtgccg ctgctggtga tcgatctctg ggagcacgcc tactatctcg atcgcaagaa
     3901 tgtgcgaccc gactacctgg aggccgtgct cgatcacgtg ctgaactggg acttcgccgc
     3961 cgagaacctg gcacgcggcc ggcgctggac ctatccggcc tgagccggcc cggcggggga
     4021 ggcgccgggc accacctgct cctccgccgc cccgccggtg gtttcctcgc ctgccgccac
     4081 ttcggggagg ttgggaaaca gcggctcgcc gaagatgaag ccgatcaggt tgggcttgcc
     4141 gagatgctcg aaagtggcgg tcaacgtaat caggatcggc accgagagca gggcgccgag
     4201 caccccccag acccaggtga aatagctgaa cgagaacagg atcagcaccg ggttcaccgt
     4261 gaaccgccgc cccaggatcg aaggcgtgac cacgttggat tcgatcgcgt gcaggcacag
     4321 gtaggccgcc ggcgcgatca gggccgggcc gatcgaggaa gcagtcgaca agccgaccag
     4381 ggcgagcacg ccgatcatcg agagcggccc gatatagggc acgaagttca gcagggccgc
     4441 tattcccccc cacatgatcg gcgcgttcca gccgagcagc catgcgccca gcgccgtgat
     4501 cgcgcccacg ccgacgttga tcatggcgac ggtgaggata taggtgccca cccggtcctg
     4561 gatttcgcgc agcagccgcg ccgccttgag gcttgagccg acgctggtgc ggtcgagcag
     4621 caggtggcgg cgcatgcgca gccggctttc gaccatgaaa taggccatga ggaaggtcag
     4681 cagggtttcc aggatcacgc tcggcgtggc gacggcgagt tgttcgatca gcgacggggt
     4741 cgccaccacc acctcgcgcg tggccgggcg gccggagacg cgggcgaggg ccttgctgat
     4801 gtcgttgagc cagtcaaagc tggagcgcag cttgacgatc tgctgggtag cattgcggac
     4861 catggccggc acgtcgtcaa ccagggcgag ggcgggctgg acgatcaggg tgatcgccac
     4921 cagcaggaac aggaccagca ccagaacggc cagcatcgag gcaatggcat tgggcaggcc
     4981 ggtgcgcagg aactggtcgg ccagcggcga gaggacgacc gagagcatca gcgcggtgac
     5041 cagcggcagg aacactaccg agccgatgga gagcacaaac ggcagcgcca ggaacaggcc
     5101 gatcccgagc agcatgacaa gcgccgaagt cagccgcaac tcctgggcgg caaagatcgc
     5161 cggatcgctg cgaacggcct cgctttccgc cgtggcgaaa ggctggcggg ccggcggcgg
     5221 cccggaatgc gtgcgtttcg caaggatttc ggcctcggtc acggcggcga tcctacgctc
     5281 tgcgtcagca ggatcaagcg acgccacggg ctatttgagc gagccgccgt gggagacgtg
     5341 ctcgagatcc ttcatgatcg cggcatgggc ggcatcttcc tcgccgtggc cgcggcgggg
     5401 aagatcgccg ctttcgatca tccgggtaag ggcggcgcgg gcgcggccca cgcggctctt
     5461 gatcgtgccg acggcgcact ggcagatttc ggcggcttcc tcgtaggaaa agccgcccgc
     5521 gccgaccagc agcagcgcct cgcgccgttc gggcgggaga gtgagcaggg cgcggtgcat
     5581 gtcggacagg tgcagcggcg cttcctgccc ggcgggcgcg gtcagcgtcc gttccgccac
     5641 ggcctcgtca tattcgccgc ggaagcggtt gcggcgcatg tcggtgagat aggcgttgcg
     5701 caggatcacg aaggtccagg cgcgcatcga ggtgccgggc tcgaaccgtt cctgggcggc
     5761 ccaggccttg agcagggttt cctggacaag gtcgtcagcg aggtcaggcc ggccgcacag
     5821 gccgcgggca aaggcacgca gatgcgggat gacctcggtc aactcgcgct tgaaagcccg
     5881 cttgtccttt tccgatctgg gagtgaagac gcgcggtgcg ccgggttggg tgggctcgtc
     5941 cgaggccggg tcagcgctca tttttcgctt tcgtccagct ttgaaatcag atcggcgaac
     6001 gtgtccggca aaggctcgtt gagcacggag tcgtaaagct gccgcaggcc gtcagcccat
     6061 tcgggacgag cgcgcttgct ctccctagat gtcgccggtc gcattggttt cttggccttg
     6121 tcgctcgtca ttttgtccgc gtgcccgtcc ctgttgctcc tggcgccccc gcaagggcgc
     6181 atcccccata cacgccttca ggccgccctc aatcggacgg cccgaccccc ttggaacgaa
     6241 aagggtgctg tgtggttccc gcaataagac gggcttgacc tttgccgcgc aagttctgcc
     6301 gctgacggcg gcttacgcgc aggtgaagcg gcacagggga atgcgtggaa agaagggtct
     6361 cagcaagggt tctcgcgcag cggtggtacc tgcggttccc gcgagggctg ccggtgggca
     6421 tcttccttct ggtctcgggc attaccgcgc tcagcgtcta tgcgatcgag caggtggaaa
     6481 cccagcgcgc cgaggcgcag cacaggcaga tggccggtgc ggcgtcctcg gcgatcgagc
     6541 ggttggtcaa tgccaacgcg gtctacctgc gcgccggttc gctgctgacg gccgatagcg
     6601 ccaatgcgtc tggtgcgttc agtgaattcg cgcgccagct ggataccgac cgcagcctgc
     6661 gcagcggcgg agcgctggcg tggtcggcgg tcgtcccgag cgaggaggtg ccggcgctcg
     6721 aagcccgcat gcgtgcagcc ggcgcggcga gctacaccgt ccacccccgc ccggtcggcg
     6781 gcactgtcat gccgatggtc tacgtccatc cggatagtcc cgatgcacgc aaggcgctcg
     6841 gcttcgacgt gctcagcgaa ggcgaacggc gcaccgcgct cgaccgggcc ctgcgcagcg
     6901 acaagccggt tgccagcggc acgatccagc tggccatctc cgacctggct ggccgccgca
     6961 caggcttcgg catgttcatg ccggtctacg gctcggtcca ggggcaccgg cggcttgtcg
     7021 gcgtgctctc gcgcgggttc gcggctcagt cattcctgga agcggcactc tcgggcgagc
     7081 ggcttgagga ctatggcgtg gccctttacg atgccgagag ccccggatca tggccgatgg
     7141 cgacgatggg tgagcaaagc cggcgcggag agcggatcgt gcgcgagatg gatctggcgg
     7201 gccaccgcat gcggctcgag atttctcccc cagcgccctc gaccctgtcc aatctctcgc
     7261 tgctcaccct gctgttcggc atgatggtcg ccgcgctgct gctgatcctc gcccgactgg
     7321 tcacccagca ggcggcagag gaccgggcgg cgctcgcctg gttcgaagaa caggcgtcta
     7381 tccgcaattc gctgacccgc gaactcaatc accgggtcaa gaacacgctg gccaacgtgc
     7441 tgtcgatcgt ggccctgacg cgccgccgca ccgacaacat cga
//
LOCUS       DATNBV010000409         2009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2441983,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000409 DATNBV010000000
VERSION     DATNBV010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2441983"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VN627_09765"
     CDS             complement(<1..931)
                     /locus_tag="VN627_09765"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HXD03582.1"
                     /translation="MATIGRRAYAEMFGPTTGDRVRLADTDLVIEVEDDYTLRAGGYG
                     EEVKFGGGKTIRDGMAQSQRTRAEGAMDCVMTNALIVDHWGIVKADIGIKDGRIAAIG
                     KAGNPDTQPGVDIVIGAGTEIISCEGNIVTAGGIDCHIHFICPQQIEEALASGVTTMM
                     GGGTGPATGTFATTCTPGPWNIERMLQAADAFAMNLGFLGKGNASLPAALHEQVNAGA
                     IGLKLHEDWGTTPAAISNCLDVAEETDTQVAIHSDTLNESGFVENTIAATKGRALCAF
                     HTEGAGGGHAPDILRVVGEANFLPSSTNPTMPYT"
     gene            complement(1010..1315)
                     /locus_tag="VN627_09770"
     CDS             complement(1010..1315)
                     /locus_tag="VN627_09770"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HXD03583.1"
                     /translation="MIPGELLTDPGDHPLNPGRRTLTLVVRNTAERPIQVGSHYHFAE
                     TNGALQFDRDAARGMRLNIASGTAMRFEPGQRRTVELVDLAGDRKVFGFRGLVQGRV"
     gene            complement(1318..1494)
                     /locus_tag="VN627_09775"
     CDS             complement(1318..1494)
                     /locus_tag="VN627_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03584.1"
                     /translation="MRFAEPLAIAAALLAAPISALAHGGHGLPDSHWHATDVSGFLFV
                     AVLATIAIWLARDE"
     gene            complement(1522..1824)
                     /locus_tag="VN627_09780"
     CDS             complement(1522..1824)
                     /locus_tag="VN627_09780"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HXD03585.1"
                     /translation="MELTPREKDKLLIFTAALLAERRKARGLKLNYPEAVALISAAVM
                     EGARDGKSVAQLMSEGRTVLTRDDVMEGVAEMIPDIQVEATFPDGTKLVTVHQPVV"
BASE COUNT          295 a          656 c          699 g          359 t
ORIGIN      
        1 ccgtgtacgg catcgtcggg ttggtcgacg acggcaggaa attggcttcg ccgacgacgc
       61 gcaggatgtc gggggcatgg ccgccgccgg cgccctcggt gtggaaggcg cacaaagccc
      121 ggcccttggt ggcggcgatg gtgttctcca cgaagccgga ctcgttgagc gtgtcgctgt
      181 gaatcgccac ctgggtgtcg gtttcctcgg cgacgtccag gcagttgctg atcgccgccg
      241 gcgtcgtgcc ccagtcctcg tgcagcttca ggccgatggc accggcgttg acctgctcgt
      301 gcagggccgc cggcaagctg gcgttgcctt tgccgaggaa ccccaggttc atggcaaacg
      361 catcggcggc ctgcagcatg cgctcgatgt tccatggccc cggcgtgcag gtggtggcga
      421 acgtgccggt ggccgggccg gtgccgccgc ccatcatcgt cgtcacgccc gaggccagcg
      481 cctcttcgat ctgctgcggg cagatgaagt ggatgtggca gtcgatgccg cccgcggtga
      541 cgatgttgcc ttcgcaggaa atgatttcgg tgcccgctcc gatgacgatg tcgacgccgg
      601 gctgggtgtc gggattgccg gccttgccga tggcggcgat gcggccgtcc ttgatgccaa
      661 tgtcggcctt gacgatgccc cagtgatcga cgatgagggc attggtcatg acgcagtcca
      721 tcgccccttc agcccgggtg cgctggctct gcgccatgcc gtcgcgaatc gtcttgccgc
      781 cgccgaattt cacctcttca ccgtagccgc cggcgcgcaa ggtgtagtcg tcctcgacct
      841 cgatcacgag gtccgtgtcg gccaggcgta cccggtcgcc ggtcgtcggg ccgaacatct
      901 cggcgtaggc gcgccgtccg atcgtcgcca tctcatgcct ccgacgggcc gcccctaggc
      961 aggcatgggc cccctcgggg ggcagagaac gaacgtgaac gtgggggctt catacccttc
     1021 cttgcacgag tccgcggaaa ccgaacacct tgcgatcacc cgcgaggtcg accagctcga
     1081 ccgtgcgccg ctggcccggc tcgaagcgca tcgcggtgcc cgacgcgatg ttcaggcgca
     1141 tgccacgcgc cgcatcgcgg tcgaactgca gggcgccatt ggtttcggcg aagtgatagt
     1201 gcgagcccac ctggatcggc cgctcggcgg tgttgcgcac gaccagcgtg agggtgcggc
     1261 ggcccgggtt gagcgggtgg tcgcccggat cggtgaggag ctcgccgggg atcatgctca
     1321 ttcgtcgcgc gcgagccaga tcgcgatcgt ggccagcacg gccacgaaca ggaagccgga
     1381 cacgtcggtc gcgtgccagt gcgaatccgg caggccatgt cctccatgcg cgagtgcgct
     1441 gatgggcgcg gccagcagcg cggccgctat cgcaagtggt tcggcaaatc gcatcatggt
     1501 gggctcctcg cgtgaatggg ttcaaaccac cggctggtgc accgtgacca gcttggttcc
     1561 gtcgggaaaa gtggcttcga cctggatgtc gggaatcatt tcggcgacac cttccatgac
     1621 gtcgtcgcgc gtcagcacgg ttcggccttc gctcatgagc tgggccacgc tcttgccgtc
     1681 gcgcgcgcct tccatgacgg cggcggagat caaggccacc gcctcgggat agttgagctt
     1741 gagaccgcgc gctttgcgcc gctccgccag cagcgcggcc gtaaagatca gcagcttgtc
     1801 cttttcgcgg ggggtcagtt ccatggctgc ggcggcgtca agaagtattg gcgatccatc
     1861 ttagtgcaag gctcgtgccc ggcgtggcgg gctccttccc cctccgggga aggtggcgat
     1921 gggggcgccg cggcggaatg cccccacccc tgccctcccc cggaggggga gggagcaaaa
     1981 ccaatggcac ggaacatgca cctgtgcac
//
LOCUS       DATNBV010000410         2925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4356483,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000410 DATNBV010000000
VERSION     DATNBV010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4356483"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /gene="metC"
                     /locus_tag="VN627_09785"
     CDS             complement(<1..1117)
                     /gene="metC"
                     /locus_tag="VN627_09785"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HXD03586.1"
                     /translation="MAKGPVDQLSPATRLTTAGRRPEWTGTADQPGAVVSPPVWRAST
                     HLYENTAALKAGPRVNADGRFYYGRRGAPTQWALAEALTELEPAAGADGGTMLYPSGV
                     AAIAGALFAALRPGDVLLMTDNAYDPSRALARGWLAEWGVETRWFDPLDVAGFAGQLC
                     ERTTAVLLESPGSLTMEVQDVPALAATAHERGACVILDNTWASPLGIAALSHGVDISV
                     MSLTKHVGGHSDLMMGSATAGAEWYGKLRRTAQVMGQVVSPDDAALALRGLRTMGVRL
                     ERETASALALAHWLQGRPEVARVLCPMLPGSPGHEIWQRDFTGGCGLFSVVLKGGSAE
                     ARNALIDSLSLFGIGYSWGGFESLVTPLDPGPARSATP"
     gene            complement(1130..1981)
                     /gene="sseA"
                     /locus_tag="VN627_09790"
     CDS             complement(1130..1981)
                     /gene="sseA"
                     /locus_tag="VN627_09790"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HXD03587.1"
                     /translation="MESLVSVDWLAGEIGACDLRIVDASKHLPEAGRDARAEYEDGHI
                     PGAVFMNLDDLVDPAAPIENTLPLPDGFASRMEALGIGDGSRIVLYDDSAVKTSARAW
                     FMLRMFGAHHVAILDGGLQAWIAAGQPLDKGVETRRHRHFTAWSDPAQVRSKAQVLAN
                     IASKAEQVIDARGAPRFTGAVPESRPGLASGHIPGSLNVPYTALYNSDGTFKSPADLR
                     AVFAAAGVDLSRPVITSCGSGVTACVVAFALHLIGKDDVALYDGSWSEWGADPALPKA
                     VGAAVPA"
     gene            complement(2142..>2925)
                     /locus_tag="VN627_09795"
     CDS             complement(2142..>2925)
                     /locus_tag="VN627_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03588.1"
                     /translation="AAPSAAPAQAEPELAPPTALPPRAEQPPAPETTTATIAWWWLLP
                     AALLGALAALAGWAVMGRRRGRQAPEPDFIRPRPVDSALPPQPPAPQPQPAASPPAAA
                     AEPLEFLLEPLRFSVTLVNATLQYRLTLANRSAATLGPIYIAADMIAAHASLPEASQL
                     AQDGAGLELRHEVAALGQGETVELKGDLRLPLAAVTPIRAGNASLLVPLVRLRVEGAG
                     PSRTTALVVGEPPVTPGGPLRPFRLDQGPRIFAAVSQRALAA"
BASE COUNT          438 a          958 c         1127 g          402 t
ORIGIN      
        1 acggcgtggc cgagcgcgcc gggccgggat cgagcggggt gaccaggctt tcgaacccgc
       61 cccacgaata gccaatgccg aagagggaca ggctgtcgat cagggcgttg cgggcctcgg
      121 ccgagccgcc tttcaggacg accgagaaca gcccgcagcc gccggtgaaa tcgcgctgcc
      181 agatctcgtg cccgggcgag ccggggagca tggggcacag gacgcgcgcg acttcggggc
      241 ggccctgcag ccagtgggca agggcaagcg cgctggccgt ctcgcgctcc agccggacgc
      301 ccatggtgcg caggccgcgc agggcgaggg cggcatcgtc cgggctgacg acctggccca
      361 tgacctgcgc cgtgcgccgc agcttgccgt accattcggc cccggccgtg gcgctgccca
      421 tcatcaggtc ggaatggccg ccgacgtgct tggtcaggct cattaccgaa atgtcgacgc
      481 cgtgggagag agcggcgatg ccaagggggc tggcccaggt gttgtcgaga atgacgcagg
      541 caccgcgttc gtgggcagtg gcggcaaggg cggggacgtc ctgcacttcc atggtcagcg
      601 agccggggct ttcgaggagc acggctgtcg tgcgctcgca aagctggcct gcgaagccgg
      661 ccacgtcgag cggatcgaac cagcgggttt ccactcccca ttccgccagc cagccccggg
      721 ccagcgcgcg gctggggtca taggcgttgt cggtcatcag gaggacgtca cccgggcgca
      781 gggcggcaaa cagcgcgccg gcaatcgccg caacccccga ggggtaaagc atggttccgc
      841 catcggcgcc ggcggcgggt tcgagttcgg tcagcgcctc ggccagggcc cactgggtgg
      901 gggcgccgcg gcggccataa tagaaccggc catcggcatt gacgcgcggg ccggccttga
      961 gggcggcggt gttttcatag aggtgggtcg acgcgcgcca gaccggcggg ctgaccacgg
     1021 cgccgggctg atcggccgtg ccggtccatt ccgggcggcg gccggccgtg gtcaggcggg
     1081 tggcggggga gagctggtcg acgggtcctt tggccatggc gcctgcctat caggcgggaa
     1141 cggccgcgcc taccgccttc ggcagtgccg gatcggcgcc ccattcgctc caactgccgt
     1201 catagagggc gacatcgtcc ttgccgatca ggtggagggc gaaagcaacc acgcaggcgg
     1261 ttaccccgct gccgcaggag gtgatcaccg ggcgggagag atcgaccccc gcggcggcga
     1321 acaccgcccg cagatcggcc ggggacttga atgtgccatc ggaattgtaa agcgccgtgt
     1381 agggcacgtt gagcgagccg ggaatgtggc ccgaggcgag gcccggccgg ctttccggca
     1441 cggccccggt gaaacgcggc gcgccgcgcg catcgatcac ctgctcggcc ttgctggcga
     1501 tattggcgag gacctgggcc ttgctgcgca cctgcgccgg atcggaccag gcggtgaaat
     1561 ggcggtggcg gcgggtctcc acgcccttgt ccagcggctg cccggccgcg atccaggcct
     1621 ggagcccgcc gtcaaggatc gccacgtgat gggctccgaa catgcgcagc atgaaccagg
     1681 cgcgggccga ggtcttcacc gcggaatcgt cataaagcac gatgcggctg ccatcgccga
     1741 tgcccagcgc ctccatccgg ctggcgaaac catccgggag cgggagggta ttttcgatcg
     1801 gggcggccgg atcgacgagg tcatcgaggt tcatgaacac cgcgccgggg atgtggccat
     1861 cctcgtattc ggcgcgcgcg tcccgtccgg cctcgggcag gtgcttggat gcgtcaacga
     1921 tcctgaggtc gcaggcacca atctcgccgg ccagccaatc gaccgacacg aggctttcca
     1981 tcaacgcggc tcctccacct cgctgcgtgt gcgaacggac tagccccgac gcggctccgc
     2041 gtcgagaggg ttccacaacc gattagcaaa gcccgcatga atgtggcgtg agaggaaacg
     2101 gtgccgccca cccgctgagg ttcggcgaac cgaactggca gctatgctgc gagcgcgcgc
     2161 tggctgacgg cggcgaagat gcggggcccc tggtcgaggc ggaaggggcg cagcgggccg
     2221 ccgggagtga ccggcggttc acccaccacc agagccgtgg tgcgcgaggg gccggcgccc
     2281 tcgacccgca gccgcaccag cggcacgagc aggctggcgt tgcccgcgcg gatcggcgtg
     2341 acggcggcga gcggcaggcg caggtcgcct ttcagttcga cggtttcccc ctggccgagg
     2401 gccgcgacct cgtggcgcag ttccaggccg gcaccgtcct gcgcgagctg gctggcctcg
     2461 ggcagcgagg cgtgggcggc aatcatgtcg gcggcgatgt agatcgggcc gagcgttgct
     2521 gccgagcggt tggcgagggt cagccggtat tgcagggtgg cgttgacgag ggtgacgctg
     2581 aaccgcagcg gctcaagcag gaattcgagc ggttcggcgg cagctgccgg cggagaagcg
     2641 gcgggttgcg gctggggggc cgggggctgc gggggaaggg cgctgtccac cggccgcggg
     2701 cggatgaaat ccggctcggg cgcctgtcgg ccccgcctcc ggcccatcac ggcccacccc
     2761 gccagcgccg ccagggcacc aagcagggcg gcgggcagca gccaccacca ggcgatggtg
     2821 gctgttgtcg tttccggcgc gggtggctgc tcggctcgcg gcggcagggc ggtcggcggc
     2881 gcgagttcgg gttcggcctg ggctggagcg gcggatgggg cggca
//
LOCUS       DATNBV010000411         2385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4357152,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000411 DATNBV010000000
VERSION     DATNBV010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4357152"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..585)
                     /locus_tag="VN627_09800"
     CDS             complement(145..585)
                     /locus_tag="VN627_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HXD03589.1"
                     /translation="MSEPIKYGPALLLPGEQLHIAMNNDTEGFWQAAKEHRLTACQCA
                     KCGHFRMPPGPVCPECSSFEKHYPDLPGTGTVFSYAICNRNPANGEPYVYVPVVVDID
                     GAPGARLVSNVTGCDAEDVHIGMKVEVVWTEINDGWVFPHFRKA"
     gene            complement(588..1805)
                     /locus_tag="VN627_09805"
     CDS             complement(588..1805)
                     /locus_tag="VN627_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HXD03590.1"
                     /translation="MSWAHMDKTAIVGVGATDYYVRGHSWPRTVYDMACEAIINACAD
                     AGISPHQIDGFAYYSGASAGYTGKLDTGTFMEMLGMPEIGFSAALTSGGNGSVGSVGL
                     ATAGLMNEDCKYCVSLMTLQQLPQSRLGTVFGKMDVDWQNSFLQPSGLVGPGHLMSVL
                     ARRHMHLYGTTQEAFGEVAIATRRNTANRPKAVRKKPLSMDEYMASPMLADPLRRLDF
                     CLETDGAVAVITTTADRAKDCRQKPAYVHGVSAGGQREWSRAFTWYGMPDPYFASSGH
                     DWPAKRVWEQTGLSAKDMDVALLYDHFTPMVLMQLEEYGFCEKGEGNDYVLSGKMRYD
                     FETGKGVNGGTPVNTHGGNLNEAYIIGMTHIVEAVEQVRGTAINQVKDCEFALASGGP
                     ASIPVSSLILRSA"
     gene            complement(1876..>2385)
                     /locus_tag="VN627_09810"
     CDS             complement(1876..>2385)
                     /locus_tag="VN627_09810"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HXD03591.1"
                     /translation="GFYARRPDVSDAIATINRYLRLQGAGLRDLRVLHSAQVAAARMA
                     SASSDETLRAGLGSLLGEMDHCDDAISMMRFDIRLAEHIAVMSGNPVVQVVTGMSYAF
                     GRDEQGIYLYGDAAQRAEVRRHYRALVDAILAGDGHLAEFHMERRLALIHGWIDAADE
                     ADLRPINEV"
BASE COUNT          395 a          783 c          779 g          428 t
ORIGIN      
        1 tgctgccgct cgcgtcggcc aacacggctc tggcccgtct gcgcgaggga gaggtcgaag
       61 gcgcgctggt gctggtgccc tgaaacgcga agcggcccgg cgggacagtc ctccgccggg
      121 ccgctgcgtc agccgcgggg cggctcaggc cttgcggaaa tgcgggaaga cccagccgtc
      181 gttgatttcg gtccacacca cctcgacctt catgccgatg tgaacgtcct cggcgtcgca
      241 gccggtgacg ttggagacga gccgggcgcc gggcgctccg tcgatgtcga ccaccaccgg
      301 cacgtagaca tagggctcgc cattggccgg attgcggttg cagatcgcat agctgaacac
      361 ggtgccggtc cccggaaggt cgggatagtg cttctcgaag ctcgagcatt ccgggcacac
      421 aggccccgga ggcatgcgga agtgcccgca cttggcacac tggcaggcgg tcaaccggtg
      481 ttccttggct gcctgccaga aaccttcggt atcattgttc attgcgatgt gaagctgctc
      541 gcccggcagc agcagcgccg gaccgtactt gatgggttcg ctcatggtca ggcgctcctc
      601 aggatcaggc tggagacggg aatggatgcc gggccgccgc tggccagggc gaactcgcag
      661 tccttcacct ggttgatggc cgtgccgcgc acctgctcca ccgcctcgac gatgtgcgtc
      721 atgccgatga tgtaggcctc gttgaggttg ccgccatggg tattgaccgg cgtaccgccg
      781 ttcacgccct tgcccgtctc gaagtcgtat cgcatcttgc cggagaggac gtaatcgttc
      841 ccctcgccct tctcgcagaa accatattcc tcgagctgca tcagcaccat cggggtgaag
      901 tggtcataga gcagggccac gtccatgtcc ttggcggaca ggccggtctg ctcccacacc
      961 cgcttggccg gccagtcgtg cccggagctg gcgaagtagg gatccggcat gccataccac
     1021 gtaaaggcgc ggctccattc gcgttgaccg ccggcggaga cgccatggac gtaagccggt
     1081 ttctgccggc agtccttggc gcggtcggcg gtcgtggtga tcacggctac ggcgccgtcc
     1141 gtctccaggc agaagtcgag ccggcgcagt ggatcggcca gcatgggcga ggccatgtat
     1201 tcatccatgg acagcggctt cttacgaacc gccttgggcc ggttagcggt attgcgccgg
     1261 gtggcgatgg ccacttcgcc gaaggcttcc tgggtcgtgc catagaggtg catgtgccga
     1321 cgggcgagca cgctcatcag gtgtccgggt ccgaccagcc cgctcggctg gaggaagctg
     1381 ttctgccaat cgacgtccat cttgccgaac accgtgccca gccgcgactg cggcaattgc
     1441 tgcagggtca tcaggctgac gcagtatttg cagtcctcgt tcatcaggcc tgccgtggcg
     1501 agcccgacgg aaccgacgct gccattgccg cccgaggtca gcgcggcgga aaacccgatc
     1561 tcgggcatgc ccagcatttc catgaaggtg ccggtatcga gcttgccggt atagccggcg
     1621 ctcgcaccgg aatagtaggc gaagccgtca atctggtgcg gcgagatgcc ggcatcggca
     1681 caggcgttga tgatcgcctc gcaggccata tcgtagaccg tgcgcggcca gctgtgcccc
     1741 cggacataat agtccgtggc gccgaccccg acgatggccg tcttgtccat gtgcgcccaa
     1801 ctcattccaa ctcccttcgt tgttctgatg tcgctgcctt gccggaaccg cgcggcagcc
     1861 cggttccgtc aggtcctaga cttcgttgat cgggcgcaga tccgcctcgt cggccgcatc
     1921 gatccagccg tgaatgaggg cgagccggcg ctccatgtgg aattcggcga gatggccgtc
     1981 acccgcgagg atcgcgtcga ccagcgcgcg atagtgacgt cgcacctcgg cccgctgggc
     2041 ggcatcgcca taaagataga tgccctgctc gtcgcgcccg aaggcatagc tcatgcccgt
     2101 caccacctgg accacgggat tgccgctcat caccgcgatg tgttcagcca gccggatatc
     2161 gaagcgcatc atcgaaatgg cgtcgtcgca atggtccatc tcgcccaaca gtgagcccag
     2221 ccccgcccgc aaggtttcgt cggaactggc cgaggccatg cgcgcggcgg cgacctgggc
     2281 cgagtgcagc acgcgcaggt cgcgaagccc cgcaccctgc agccgcaagt agcggttgat
     2341 cgtggcgatg gcgtccgaca cgtcgggccg ccgggcatag aaccc
//
LOCUS       DATNBV010000412         2595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4357215,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000412 DATNBV010000000
VERSION     DATNBV010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4357215"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..540)
                     /locus_tag="VN627_09815"
     CDS             complement(61..540)
                     /locus_tag="VN627_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03592.1"
                     /translation="MSILESGRIYPLGLPSQDLIHIEASDARAALWAMEEGVRCSGLS
                     CVIGEIWGDPKVLDFTATRRLAVASERSGVPCWLVRLGGSANLSGARMRWRIASAPSL
                     VNPLDPRAPGGSAWDAELFRARGAPPGRWSLTHEADRFHLVAASGDRALGEERRLTA"
     gene            complement(865..1536)
                     /locus_tag="VN627_09820"
     CDS             complement(865..1536)
                     /locus_tag="VN627_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXD03593.1"
                     /translation="MTEAAWLAPRLWSTFKRSRTLGPEDGPPALVIPGWVASDRTTRA
                     LRRALADAGWRVYGWKLGFNRGATSDTVERLKRRLEEVGRGRPVLLVGWSLGGVYARE
                     LARAAPEGVLAVVTLGSPFSGDPKQNNVWRLYEWIAGHKVDEPPIPRITDKPPVPHLA
                     LWSRRDGIIAPRSARGLDHERDKAVEMRCTHMAFGVSGRLTREVVREIDVFLEELGIA
                     PESQH"
     gene            complement(1584..>2595)
                     /gene="serS"
                     /locus_tag="VN627_09825"
     CDS             complement(1584..>2595)
                     /gene="serS"
                     /locus_tag="VN627_09825"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXD03594.1"
                     /translation="EDQLAGEEAALNAMMLQLPGIPYEGAPVGPDETYNAVIRTEGEP
                     PKFDFEPLDHVALIEKNDWADLSRVTQVSGSRTYCLKGALALLEAKLMAWALEKIAAA
                     DFTPITVPAIAREQAFLNQGQFPGHREETYELPNDDLWLAGTAEVVLTSLHSGEIIEA
                     DKLPILYAGFSPCFRREAGSAGKDVRGLLRVHQFMKVEQYVICEADETQSAEWHATLL
                     RLAEELLQAMEIPYQVIETSTGDMGLGKYRMNDIESWVPSLAKYRETHSCSTLHDWQA
                     RRANIRYRGSDGKVRFAHTLNNTALASPRILVPLLENHQTSDGRVKLPKALRELMGGD
                     YL"
BASE COUNT          447 a          877 c          827 g          444 t
ORIGIN      
        1 ttcgtgacgg cgtgaatgat gaggccgtgc gggccctcga gcgtgaggac gacggggctg
       61 tcaggcggta agtcggcgct cttcgcccag cgctcgatcg ccagatgcgg caaccagatg
      121 aaagcgatcc gcttcatgcg tgaggctcca gcggcccgga ggcgccccac gcgcgcggaa
      181 cagctcggca tcccacgcgg agcctcccgg cgcgcgtggg tcgagcgggt tcacgagcga
      241 gggcgcgctc gcgatccgcc agcgcatccg cgctccgctc aagttcgcgc tgccgccgag
      301 gcgcaccagc cagcagggaa cgccgctccg ctccgatgcc accgccagcc gccgggtcgc
      361 ggtgaaatcg agcaccttcg ggtcgcccca aatttcgccg atgacgcagg agaggcccga
      421 gcatcttacg ccttcctcca tcgcccacag cgcagcacga gcatcgctcg cctcgatatg
      481 gatgaggtcc tgcgacggca ggccaagcgg atagattcgt cccgattcga ggatcgacat
      541 gcggtcctgg acccagagca agggtttcga acgatcgatc tgggccagga ggaagcccgt
      601 ccagcccccg tcgcgcgggt gtgcggcgaa gagttcggaa agggtgggtt tcctcccctc
      661 gatttcgagg ggagggggac cgccgtaggc ggtggagggg cccctccgac gcgctgcgcg
      721 cgccacctcc cctgcaaatg caggggagga gacatcaggc cgtgcggaga cagctccgcc
      781 ggcgaccaga gacaagcctc ttgccacacg actcacaacg tttcgttctg cttttgttct
      841 tgtctcgcga ttcaggggca agagtcaatg ctgcgactcg ggcgcgatcc caagttcttc
      901 taaaaacacg tcaatttcgc gaacgacttc gcgagtcagc cggccagaaa cgccgaatgc
      961 catgtgcgtg cagcgcatct cgaccgcctt gtctcgctca tggtcgagac cgcgagcgga
     1021 gcgcggagca atgatcccat cccgccgcga ccatagggca agatgaggca ccggaggttt
     1081 gtcggtgatc ctggggatcg gcggctcatc gaccttatgg ccggcgatcc attcgtagag
     1141 ccgccacacg ttgttctgct tggggtcgcc cgagaaggga gagcccaaag tgacgacggc
     1201 cagcactccc tccggggccg cccgcgccag ttcgcgggca taaactccgc cgaggctcca
     1261 accgaccagc agaaccggcc ggccgcggcc cacctcctcc agccgacgct tcagccgttc
     1321 caccgtgtcg gaagtcgctc cacggttgaa gcccagcttc cagccataaa ctcgccaccc
     1381 cgcatcggcg agcgccctgc gcagcgccct cgtcgtacgg tcgctcgcca cccagcccgg
     1441 gatgaccagc gccggcggac cgtcttcggg gccaagcgtc cggctgcgct taaaggtgct
     1501 ccatagacga ggagccagcc aagccgcctc ggtcaatcgc cgccagtttg gcggaggccg
     1561 atcatcgcct gtgaaatgat gcttcagaga tagtctccgc ccatcaattc ccggagcgct
     1621 ttcggcagct tcacccggcc gtcggaagtt tgatggttct cgagcagcgg cacgaggatc
     1681 cggggagaag cgagggccgt attgttcagc gtgtgcgcga accgcacttt gccgtcgctc
     1741 ccgcggtaac ggatgttcgc gcgccgcgcc tgccagtcgt gcaaggtcga gcagctgtgg
     1801 gtctcgcgat atttggccag gctcggcacc cagctttcga tgtcgttcat gcgatatttg
     1861 cccagaccca tgtcgccggt cgatgtctcg atcacctggt acggaatctc catcgcctgg
     1921 agcagttctt cggccagccg aagcaaggtc gcgtgccatt cggcggactg ggtctcgtca
     1981 gcctcgcaaa tcacatattg ctcgaccttc atgaactggt ggacccgaag cagcccgcgc
     2041 acgtccttgc ccgcgcttcc cgcttcgcgc cggaaacagg gcgagaaacc ggcgtagagg
     2101 atcggcagtt tgtcggcctc gatgatctcg cccgaatgga gcgaggtgag gaccacctcg
     2161 gcggtccccg cgagccacag atcgtcgttc gggagctcgt aggtctcctc gcggtggccg
     2221 gggaactggc cctggttcag aaaggcctgt tcgcgcgcga tcgccggcac cgtgatcgga
     2281 gtgaaatcgg ccgccgcgat cttctccagc gcccaggcca tcagcttcgc ctcgagcagc
     2341 gccagcgcgc ccttcaggca gtaagtccgg ctgccggaca cctgggtcac ccgcgacagg
     2401 tccgcccagt cgttcttctc gatcagagcg acatggtcga gcggctcgaa atcgaacttc
     2461 ggcggctcgc cctcggtgcg gatcaccgca ttgtaggttt cgtcgggacc caccggtgcc
     2521 ccctcgtagg ggattccggg gagctgcagc atcatggcgt tcagggccgc ttcctcaccg
     2581 gccagctggt cctcg
//
LOCUS       DATNBV010000413        11543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2795476,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000413 DATNBV010000000
VERSION     DATNBV010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2795476"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..1077
                     /locus_tag="VN627_09830"
     CDS             340..1077
                     /locus_tag="VN627_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HXD03595.1"
                     /translation="MTATRLFSRAVVRLSPEAGEDAAGFLQGLVTNDITGVLPVWCGL
                     LTPQGKALFDFIVWPSGDDFLLDCEAAAADALVRRLSLYRLRRKIAIGVDHNIAVHWR
                     GHIGDGASPDPRLAALGERWIAPLDADDAESADAEWRAHRLKLGVLEGRSELGDGQTL
                     WLECNAAELNGVSFTKGCYVGQENTARMNWRQKVNRRLLVVPLDRSQADRQRAAYPEL
                     GLAIDHVRVSDIAPELLPDWLQLDEPA"
     gene            complement(1074..2216)
                     /locus_tag="VN627_09835"
     CDS             complement(1074..2216)
                     /locus_tag="VN627_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03596.1"
                     /translation="MGCTRARGQPLVALAVILAGWVGARAVAWNLLPGAPEPVIIAAM
                     DDVPSPLPLPETAPRHGMHAAPVRRAPLIAPRPLPAVLPPAWTAAPPPALLPPPPVPL
                     ALTSARSDPLPAAEAGANGQTLVAGGHQLLWLAAAAMLPLPADVAARFAAAPPPRTAS
                     PPRWSADGWVFLRRGGVPVTFPRLSDGGTYGASQAGAVLRYRLALDDPHRPTVYLRGA
                     GALNGTREQEAALGLAARPLAGFPVAAMAEVRLTRNAQGTSARPAIALVTELPPQRLP
                     LGFTGEAYVQGGYVGGRGGTAFVDGLLRAERGLVDLAGAEVKAGAGAWGAKQRGASRL
                     DVGPVAAVRLRLGDTASARLEADWRFRVAGKAHPGSGPALTLSAGF"
     gene            complement(2216..3685)
                     /locus_tag="VN627_09840"
     CDS             complement(2216..3685)
                     /locus_tag="VN627_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HXD03597.1"
                     /translation="MLGAQLAAAAFVAGALPLLQVAQYELTLFAGFWFIVGMIDEMAV
                     DAVWGWLLLRRRVRSPRLVDGLAHRELAGIAAVFIPAWHEAGVIGATVAHALKAWPQA
                     DLRLYVGCYRNDPGTVAAVMAEAGHDPRVRLVVHDRDGPTTKADCLNRLYGTLCEDEL
                     RSGRRARSVVLHDAEDMVHPAALFVLDAALHDASFVQLPVRPEPQRGSRWVAGHYGDE
                     FTESHAKAMVVRDALGAALPAAGVGCAIARNVLTDLARERKSELAREQGAIFTRQAGP
                     FASECLTEDYEMGWRIARGGHRARFLRLRDGEGQLIATRSYFPASIDAAVRQKARWVH
                     GIAFQGWDRLGWGGRAVDLWMAARDRRGPLVALVLFVAYVLVVLGGVIAAAESFGLVR
                     PLSQPPELRLLITLCLVGLAWRMVMRFVFTAREYGAAEGLMAVLRIPVANIIAIVAGR
                     RAVAAYVRSLAGSAVRWEKTAHHGHPTLRQPAIPGEARA"
     gene            3840..5006
                     /gene="nhaA"
                     /locus_tag="VN627_09845"
     CDS             3840..5006
                     /gene="nhaA"
                     /locus_tag="VN627_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HXD03598.1"
                     /translation="MLVLARGEAGAGLLLIAAAAVALILANSPLAGRYHAVFTGPILY
                     TPIAKLATLDLWVNDALMAVFFFVVGLEIKREVLDGELASAARRRLPVLVALAGMAVP
                     ALVYLAIAGAGPGLRAGWAIPAATDIAFAVGVLALLGRRAPPALRLLLLTVAIVDDLG
                     AVAMIALAYTPSLALGWLAAAGGLLAIMALLGRRGVDLWWPYVLLGALLWYAVLCSGV
                     HATVSGVLAAFTVPLRPGRWRGDSLLLRFEHGLAPWSAYLIVPLFGFANAGVHLGGEG
                     LAAALAPLPLAVGAGLFIGKQAGVLGALALARATGFAKLPAGATWLQLWGLAALCGIG
                     FTMSLFIATLAFPAAPELVDAAKLGILGGSALSAVLGYALLRFSGSAKPAVAPD"
     gene            complement(5087..5524)
                     /locus_tag="VN627_09850"
     CDS             complement(5087..5524)
                     /locus_tag="VN627_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXD03599.1"
                     /translation="MTKYLHTMIRVTDPEATVRFFNLIGLEEVRRFDSPQGRFTLIFL
                     APPGQAGVAEVELTHNWPPEDGSPGEAYGGGRNFGHLAFQVDDVYATCQRLLDAGVTI
                     NRPPRDGHMAFVRSPDGISIELLDKDRQPPAEPWASMPNVGEW"
     gene            complement(5547..6308)
                     /locus_tag="VN627_09855"
     CDS             complement(5547..6308)
                     /locus_tag="VN627_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HXD03600.1"
                     /translation="MNPATRLLSALLAAAALAPTAATAQDVPEPRALTVSGNVALVSD
                     YRFRGVSQTGGDPAVQGGLTLTHESGVYAGVWASSIDFAKLGAAATYGSTEVDLTAGW
                     SRDIASGITADLGLIYYAYPGGHVGKANFLETYGSLATTYGPARIKLGANYAWPQDSL
                     AGSHNLYVYGSADAAIPATPVTVSAKLGYQDGPLSAAWIATGGARQSGWDWSLGASAT
                     VLGKLTLGASYIGSGGPSLSGVTDDKLVASLTVAF"
     gene            6411..7298
                     /locus_tag="VN627_09860"
     CDS             6411..7298
                     /locus_tag="VN627_09860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXD03601.1"
                     /translation="MSDRGQDTNHAAVVRSASAPYTAPQPATVMLNALKKLLRAQPER
                     RSAAIPAGQRVYAVGDVHGRADLFTRLAAAIDADDAAREPAETTVILLGDLIDRGPDS
                     AGVIAAARAWGERRRVRYIGGNHEELLLKALDDVDYLRVFLRYGGRETLLSYPLCPRE
                     YQVAEFDQVQAMMAAAIPAEDIAFVSAFEDAIRIGDYLFVHAGVMPGIDLDQQQTGNL
                     RWIREPFLSYPGDHGAVVVHGHTITDDVEVRPNRIGIDTGAYYSGRLTALALEGTDRR
                     LIEARETDGLFAIHTRSIA"
     gene            7295..8605
                     /locus_tag="VN627_09865"
     CDS             7295..8605
                     /locus_tag="VN627_09865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HXD03602.1"
                     /translation="MKIAMVGSGYVGLVSGACLADFGHDVVCIDKDPGKIERLNAGVM
                     PIYEPGLAELVASNVKAGRLSFTTSLAEGIDGAQAIFIAVGTPSRRGDGHADLSYVYG
                     VAQEIAENLKNPAVVVTKSTVPVGTGDEVERIIGAANLPHRVAVVSNPEFLREGAAIG
                     DFKRPDRIVIGAEDEFGQEVMREVYRPLFLNQAPLLFTSRRSSEMIKYAANAFLATKI
                     TFINEIADLCEKVGGDVQDVARGIGLDNRIGSKFLHAGPGYGGSCFPKDTLALLKTAE
                     DYDSPLRIVEAVAKVNETRKRAMGRKVLEAIGGSDGARGKKVALLGLTFKPNTDDMRD
                     APSIAIAQTLQDAGVDVVAYDPEGMEMAAPLMPGVTMAKSTYDAVTGADAIALVTEWD
                     AFRALDLDRVKSQAKGAVLVDLRNVYRPDEVRAKGFAYSSIGRV"
     gene            complement(8699..10954)
                     /locus_tag="VN627_09870"
     CDS             complement(8699..10954)
                     /locus_tag="VN627_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXD03603.1"
                     /translation="MKSRNLLCQVASIALAFGAQAAHAAGADQSAQAPAAAAAGDDIV
                     VTARRREERLQDVPVAVTAISAKAIDQRGIKTTEDLRYSVPSLQVAPSPFGSVVPGYT
                     IRGQRQLEQLITQDPSVGVYFADVVQQRPHGTNSAVYDLASVQVLKGPQGTLFGRNTT
                     GGAVLFVPNKPTFELGASAAVSIGDYNLRRGTVVLNVPLSSTLAVRAAGQITRRDGYN
                     LNLTTNTRTDDERTESARLSILWEPGNGISNETIGTYFHENDAGNGFALTAVRAGSTA
                     GKTAGTFEALARQQARSIHIIENDWAPSAFVDSYQLINTTKIPLSDEVTLKNIVGFRH
                     VHSNVVLDFDGGPTTVFHTANDLNADQFTNELQLSGTTPDKRLNWIGGLYYFRESGDD
                     VQKSFLGGDRINDGFARNYSISGYLQGGYKLTDQLTLTAGGRYTHDTRYLNARSKIGN
                     PPTCRLTDANGAKLNPCTKEVSTQFNSPTWTLSLDYKPNDDTLLYLAHRRGYRSGGWN
                     LRSLAPINFVPFKPETVYDVEGGVKVDLFDKRLRINVAVYHQWYKDIQRTISAVVPGT
                     NPPTVATNVVNAAAAKIYGGEVEITARPIDLIEIGANAAFIHEKYDRFADTIGDLSYN
                     RFTLVPRFQGSAYVRLNLPLASDQGDAGMQLGVYHQSSQEASDINTPFPIKPYTLLDF
                     SANWNRVMGSPVDLSVFVKNITDKEYTTSAISQYGDKFGTHVGIGAQAESIGAPRTFG
                     MEVKVHFGAGV"
     gene            complement(11185..>11543)
                     /locus_tag="VN627_09875"
     CDS             complement(11185..>11543)
                     /locus_tag="VN627_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HXD03604.1"
                     /translation="RLIPSGLGVGLLAGFFGIGGGFLIVPALIMATGMPLRIAVGTSL
                     VAVFALGLTTATSYALSDYVEWQLVGLLVVGGVAGALAGIAFGKRLAGAKRKMEFGFA
                     ALVITVGCYVVARGLG"
BASE COUNT         1895 a         3802 c         4001 g         1845 t
ORIGIN      
        1 ttgatccggg cgagatcggg atcggtcatc agctgttcgt tcagggcacc ggaaattgcc
       61 gggtcctgct cggcacgttc cttgcgcggc tggtcgggcg cgcaggcggc cagcccggca
      121 aacagggcca gtgcaaggac cgaggcggga gcgacgcaac gcatggggct tcctagcaaa
      181 cggcgcaggt caaggccaag cgttccgcct tgcgggacag gtagcgttcg cagccacctt
      241 ggcctatcgt tttccgggat cggggggaac tgccttgccg cacctcccgt tgcccgaatg
      301 aacgccgccc gaaccctgcc tgcaaccagt gacctgccca tgaccgcaac ccgcctgttt
      361 tcccgcgccg tcgtccgcct ctcgcccgaa gccggggagg atgccgcggg attcctgcaa
      421 gggctggtca ccaatgacat taccggcgtg cttccggtct ggtgcgggct gctgacgccc
      481 cagggcaagg ccctgttcga cttcatcgtc tggccttcgg gcgacgactt cctgctcgac
      541 tgcgaggccg cagcggccga cgccctggtg cggcggcttt cgctctaccg gctccggcgc
      601 aagatcgcga tcggggttga tcacaacatc gccgtccact ggcgcggcca catcggcgac
      661 ggcgcctcgc ccgatccgcg gctggcggcg ctgggtgagc ggtggatcgc tccgctcgat
      721 gccgacgacg cggaaagcgc cgacgccgaa tggcgcgccc atcggctcaa gctcggcgta
      781 ctcgaaggcc ggagcgaact tggcgacggg cagacgctat ggctcgagtg caatgcggcc
      841 gagctcaacg gggtgagctt caccaagggc tgctacgtcg ggcaggaaaa caccgcgcgg
      901 atgaactggc ggcagaaggt gaaccgtcgc ctgctggtgg tgccgcttga ccgctcacag
      961 gcagaccggc aacgcgcggc ctatcccgaa ctgggcctgg ccatcgatca cgtccgggta
     1021 agcgacatcg cccccgaact gctgcccgac tggctgcaac tggacgagcc cgcttagaac
     1081 ccagccgaca gcgtcagcgc cggcccggaa ccggggtgcg ccttgccggc cacgcggaag
     1141 cgccagtcgg cctcgagccg ggcactggcc gtatcgccga ggcggagtcg gacagcggcg
     1201 accgggccga cgtcaagccg gctcgccccc cgctgcttgg cgccccacgc ccctgccccg
     1261 gccttgacct cggccccggc cagatcgacc agcccgcgct cggcccgcag cagcccgtcg
     1321 acgaaggccg tgccgccccg cccgccgaca tagccgccct ggacataggc ctcgccagtg
     1381 aagccgagcg gcagccgctg cggcggcagt tcggtgacga gggcgatggc cgggcgggca
     1441 ctggtcccct gggcgttgcg ggtgaggcgt acctcggcca tggcggcgac cgggaagccg
     1501 gccagcggcc gcgccgcgag gccaagggct gcttcctgtt cgcgcgtgcc gttgagcgcg
     1561 ccggcgccgc gcaggtagac ggtgggccgg tgcggatcgt ccagagcgag gcggtagcgc
     1621 aggactgcgc cggcctggct cgcgccatag gtgccgccgt cgctgaggcg cgggaaagtt
     1681 accggcacgc cgccccggcg caggaagacc cagccatcgg ccgaccagcg cggcgggctg
     1741 gcggtacgcg gcgggggagc ggcagcgaag cgggcggcga cgtccgccgg cagcggcagc
     1801 atggcggcgg cggcgagcca cagcaactgg tgcccgccgg cgaccagtgt ctgaccgttg
     1861 gccccggctt ccgccgccgg caggggatcg gaccgggccg aagtgagggc aagcggcacg
     1921 ggcgggggcg gcagcaaggc cggcgggggc gcggcggtcc acgccggcgg caggactgct
     1981 ggcagcgggc gcggggcaat cagcggagcc cggcgcaccg gcgcggcgtg catgccatgg
     2041 cgaggcgcag tttccggcag cggcaatggc gacgggacat cgtccatcgc cgcgatgatc
     2101 accggctccg gcgcgccggg cagcaggttc caggcgacgg cgcgcgcgcc gacccagccg
     2161 gcgaggatca ccgccagcgc gaccagcggc tggccccggg cgcgggtgca acccatcagg
     2221 cgcgggcctc gccgggtatg gcaggctgtc gcaaggtggg gtggccgtgg tgagctgtct
     2281 tctcccaccg cacggcgctg ccggcgagac tgcggacata ggctgcgacg gcgcggcgac
     2341 cggcgacgat ggcgatgata ttcgccacgg gaatacgcag cacggccatc agcccctccg
     2401 ccgcgccata ttcgcgggcg gtgaagacga agcgcatgac catgcgccag gccaggccca
     2461 ccaggcacag cgtgatcagc aggcgcagtt ccggcggttg ggagagcggg cgcaccaggc
     2521 cgaacgactc ggccgcggca atgaccccgc cgagcacgac cagcacatag gcaacgaaca
     2581 acaccagcgc gaccaacggc ccgcgccgat cgcgcgcggc catccacagg tccaccgcgc
     2641 gcccgcccca gccgagccgg tcccagccct ggaaggcgat cccgtgaacc cagcgggcct
     2701 tctgacgcac cgcggcatcg atcgaggcgg ggaaatagga gcgcgtggct atcagctgcc
     2761 cctccccatc acggaggcgc aggaaccggg cgcgatgccc gccccgggcg atgcgccagc
     2821 ccatctcgta gtcttcggtc aggcactcgg aggcaaaggg cccggcctgc cgggtgaaaa
     2881 tggcgccctg ttcgcgcgcg agttcgctct tccgctcgcg cgcaaggtcg gtgagcacat
     2941 tgcgggcgat ggcgcagccg acaccggcgg ccggcagggc cgcgcccagt gcatcgcgca
     3001 cgaccatcgc cttggcatgg ctttcggtaa attcatcgcc atagtgcccg gccacccagc
     3061 gcgatccgcg ctgcggctcg ggccggaccg gcagctggac gaagctggcg tcatgcagcg
     3121 ccgcatcgag cacgaacagg gcggcgggat ggaccatgtc ctcggcatca tggagcacga
     3181 ccgagcgggc acggcggcca gaccgcaatt cgtcctcgca cagcgtccca tagagccggt
     3241 tgaggcaatc ggccttggtg gtggggccat cgcgatcgtg gacgacgagg cgcacccgcg
     3301 gatcgtggcc cgcctcggcc atgactgcgg cgacagtacc cggatcgttg cgatagcagc
     3361 cgacgtagag cctgagatcg gcctggggcc aggccttcaa ggcatgggca acagtggccc
     3421 cgataacacc cgcttcgtgc caggcgggga tgaacacggc ggcaatcccc gccaactccc
     3481 ggtgcgccag cccatcgacc aggcgcggcg agcggacccg gcggcgcagc aagagccagc
     3541 cccagacggc gtcgaccgcc atctcgtcga tcatgccgac gatgaaccag aagcccgcga
     3601 acagggtcag ttcgtattgt gcgacctgga gcagcggcaa ggcgccggcc acgaaggccg
     3661 ccgccgccaa ttgcgcaccc agcattgcgc cccccaaggc catgccgtca gaaaccccgt
     3721 taaaacccct aaccgagttc tgtaatcaat tgcagactcc ttgcaaactc ttaacggcgc
     3781 gccgaagaaa gcgcacgatg acgtcccgca gacacttttt ctcaaggctc gccactcgca
     3841 tgctggtgct ggcacggggc gaggccgggg cggggctatt gctgatcgca gcggctgcgg
     3901 tggcgctgat cctcgccaac tcgcccttgg ccgggcgcta tcatgcagtg ttcacgggtc
     3961 cgatacttta tactccgata gccaagctcg ccacgctcga cctgtgggtg aacgatgcgc
     4021 tgatggcggt gttcttcttc gtcgtcgggc tcgagatcaa gcgcgaggtg ctggatggcg
     4081 aactcgcttc ggcggcacgg cgacggctgc ctgttcttgt cgcgctggcg ggcatggccg
     4141 tgccggcgct ggtctacctc gccattgccg gcgcggggcc cgggcttcgc gccggctggg
     4201 ccattccggc ggcgacggat attgccttcg cggtcggcgt gctggccctg cttggccgcc
     4261 gcgccccgcc ggccctgcgc ttgctgctgc tgacggtggc gatcgtcgac gatctggggg
     4321 cggtggcgat gatcgcgctg gcctatacgc cctcgctggc gctcggttgg ctcgcggccg
     4381 ccgggggact gctggcgatc atggccctgc tcggccggcg cggggtggac ctgtggtggc
     4441 cctatgtcct gctgggcgcg ctgctgtggt atgccgtgct gtgttcgggc gtccacgcca
     4501 cggtgtcggg cgtgctggcg gcctttaccg tgcccctgcg cccgggccgc tggcgcggcg
     4561 acagcctgct gctgcgcttc gagcatggcc tcgcgccatg gagcgcctac ctgatcgtgc
     4621 cgctgttcgg ctttgccaat gccggggtgc accttggcgg agaaggcctt gcagcggcgc
     4681 ttgccccgct accgctggcg gtgggcgcgg gcctgttcat cggcaagcag gccggggtgc
     4741 tcggggcgct ggctcttgcc cgggccacgg ggtttgcgaa gctgccagcg ggggcgacct
     4801 ggctccagct gtggggcctc gccgcgctgt gcgggatcgg cttcaccatg agcctgttca
     4861 ttgccaccct cgccttcccc gccgccccgg agctggtgga cgcggccaag ctcggaattc
     4921 tgggtggctc ggcgctgtcg gcagtgttgg ggtatgcgct gttgcggttt tcaggttcgg
     4981 caaagcccgc cgtcgcgcca gactgagggg gcgcatgctg aggcgcgagt tgggatgggc
     5041 ccctcccttc gcaaggggcc acggttaatc agggcgcgga gttgtctcac cattcgccca
     5101 cattgggcat gcttgcccac ggctcggcag gcggttggcg gtccttgtcg agcagttcga
     5161 tcgaaattcc atcgggcgag cggacgaagg ccatatgccc gtcgcgcggg gggcggttga
     5221 tggttactcc ggcatccaac aggcgctggc aggtggcgta gacatcgtcc acctggaaag
     5281 ccagatggcc gaagttgcgc ccgcccccat aggcttcgcc cggcgacccg tcttcgggtg
     5341 gccagttgtg ggtcagctcc acctcggcga cgccggcctg ccccgggggc gcgaggaaga
     5401 tcagggtaaa gcggccctgc ggggaatcga agcggcgaac ttcctcgagc ccgatcaggt
     5461 tgaagaatcg cacggtcgcc tcgggatccg tcacccggat catggtatgc aggtacttgg
     5521 tcacgcaggt ctcccgggca atcggttcag aaggcgacgg tcaggctagc aaccagcttg
     5581 tcgtccgtca ccccggagag cgacggtccg cctgagccga tatagctggc gcccaaagtc
     5641 agtttgccga gcacggtcgc gctggcgccg agcgaccagt cccatccgct ttgccgcgcg
     5701 ccgcccgtgg caatccaggc tgccgacagc ggcccgtcct ggtagccgag cttggcactg
     5761 acggtgaccg gcgtggcggg aatggccgca tcggcgctgc catagacata gaggttgtgc
     5821 gaaccggcca gcgaatcctg cggccaggca tagttggcgc cgagcttgat ccgcgccggg
     5881 ccgtaagttg tcgccagcga gccgtaggtc tccaggaagt tggccttccc cacatggccg
     5941 cccggatagg catagtagat cagtccgagg tcggccgtga tccccgaggc gatgtcgcgc
     6001 gaccagccgg cggtaaggtc aacctcggtg ctgccgtagg tcgcggctgc cccgagcttg
     6061 gcgaaatcga tcgaggaggc ccagaccccg gcgtagacac cgctctcgtg cgtgagggtg
     6121 agcccgccct gcaccgccgg atcgccgccg gtctgcgata cgccgcggaa ccgatagtcg
     6181 ctgaccagcg cgacattgcc ggaaacggtc aacgcccggg gttccggcac gtcctgcgcc
     6241 gtggctgccg tgggggccag ggcagcagca gcgagcagag ccgagagcag gcgggtggca
     6301 gggttcatcg cgaatttcct cgattgtacc gagccttgtc taccgtgcgc gctgcgatgc
     6361 acaaggcgcg gcaccgcttc tggccacgag cgttggtgcc agaccattcc atgagcgacc
     6421 ggggacagga caccaaccac gcggcggttg tgcgctctgc ctcggctccc tataccgcac
     6481 cgcaaccagc tactgtcatg ctcaacgccc tcaagaaact gctgcgcgcc cagcccgagc
     6541 gccggagcgc cgccattccc gcgggccagc gggtctatgc ggtgggcgat gtccacgggc
     6601 gggcggatct gtttacccgg ctcgccgccg cgattgatgc cgacgatgcc gctcgcgagc
     6661 cggcggaaac cactgttatc ctgctgggcg acctgatcga ccgcgggccc gacagcgccg
     6721 gggtgattgc cgccgcccgc gcctggggcg agcgccgccg ggtgcgctac atcggcggga
     6781 atcacgagga actgctgctc aaggcgctcg atgatgtgga ttacctgagg gtgttcctca
     6841 ggtacggcgg gcgtgaaacg ctgctgtcct acccgctgtg cccgcgcgaa taccaggtgg
     6901 ccgagttcga ccaggttcag gcaatgatgg cggcggcgat cccggcagag gacattgcct
     6961 tcgtctccgc cttcgaggat gcgatccgca tcggcgatta cctgttcgtc catgccggcg
     7021 tcatgccggg gattgatctc gaccagcagc agaccggcaa cctgcgctgg atccgcgagc
     7081 cgtttctaag ctatccgggc gatcacggcg ctgtcgtggt ccacgggcat acgattaccg
     7141 acgatgtgga agtcaggccc aaccgcatcg gcatcgatac cggcgcctac tactcgggcc
     7201 ggctgaccgc gctggcgctg gaagggaccg accggcgcct gatcgaagcg cgggaaaccg
     7261 acggcctgtt tgccattcat acacggagca ttgcatgaag atcgcgatgg tgggatcggg
     7321 ctacgtcggc ctggtctcgg gggcatgcct ggccgatttc gggcacgacg tggtctgtat
     7381 cgacaaggat ccgggcaaga tcgagcgcct gaacgcgggc gtcatgccga tctacgagcc
     7441 gggcctggct gaactggtcg ccagcaatgt caaggccggg cgcctgtcgt tcaccacctc
     7501 actggccgag gggatcgacg gggcccaggc gatcttcatt gccgtgggca cgcccagccg
     7561 gcgcggggat ggccatgccg acttgtccta cgtctacggc gtggcgcagg agattgccga
     7621 gaacctcaag aacccggccg tggtcgtcac caagtcgacc gtgccggtgg gcaccggcga
     7681 cgaggtcgag cggatcatcg gcgccgcgaa tctgccgcac cgggtcgcag tcgtctccaa
     7741 cccggaattc ctgcgggagg gcgcggcgat cggcgatttc aagcggcctg accgcatcgt
     7801 gatcggcgcg gaggacgagt tcggccagga agtcatgcgc gaggtctacc gcccgctgtt
     7861 tctcaaccag gccccgctgc tgttcaccag ccggcggtcg tcggagatga tcaagtacgc
     7921 ggccaatgct ttcctcgcga ccaagatcac cttcatcaac gagatcgccg acctgtgcga
     7981 gaaggtgggc ggcgacgtgc aggacgtggc gcgcggaatc gggctcgaca accggatcgg
     8041 cagcaagttc ctccacgccg ggcccggcta tggcggttcg tgcttcccca aggacacgct
     8101 tgccctgctc aagaccgccg aggactatga cagcccgcta cggatcgtcg aggcggtggc
     8161 caaggtcaac gagacccgca agcgcgcgat gggccgcaag gtgctcgaag cgatcggcgg
     8221 gtcggacggg gcgcgcggca agaaggttgc cctgctcggc ctcaccttca agcccaacac
     8281 cgacgacatg cgcgacgcgc cctcgatcgc catcgcccag accctgcagg atgccggcgt
     8341 ggacgttgtc gcctatgacc ccgagggcat ggaaatggcc gccccgctga tgccgggcgt
     8401 gaccatggca aagagcacct acgacgccgt gaccggagcg gatgccatcg ccctcgtcac
     8461 cgaatgggac gccttccgcg cgctcgacct ggaccgggtg aagagccagg ccaagggtgc
     8521 ggtgctggtc gacctgcgca atgtctatcg gcccgatgaa gtgcgggcca agggctttgc
     8581 ctattcgagc atcgggcgcg tctaaacgcg gcggccgcgc ccgcctgagc ggacgcggcc
     8641 tgtattctcc gcaagggtcg caggccgggc ctttcgggcc cggccagacc tgtcaggatc
     8701 agaccccggc gccgaagtgc accttcactt ccatgccgaa ggtgcggggc gcgccgatcg
     8761 actcggcctg ggcgccaatg cccacgtgcg tcccgaactt gtcgccatac tgcgagatcg
     8821 ccgaggtggt gtattccttg tcggtgatgt tcttgacgaa caccgagagg tccaccgggc
     8881 tacccatcac gcggttccag ttcgccgaga aatcaagcag cgtgtagggc ttgatcggaa
     8941 acggcgtgtt gatgtcggaa gcttcctggc tggattggtg gtagacgcca agctgcattc
     9001 ccgcatcgcc ctggtcgctc gccagcggca ggttcaaccg cacataggca ctgccctgga
     9061 agcgcggcac cagcgtgaag cggttgtagc tgagatcgcc gatggtgtcg gcgaacctgt
     9121 catacttctc gtggatgaag gcagcattgg cgccgatctc gatcaggtcg atcggccgag
     9181 cggtgatttc cacttcgccg ccgtagatct tcgccgccgc ggcattgacc acgttggtcg
     9241 ccacggttgg cgggttggtg cccggaacaa cggccgagat cgtccgctgg atgtccttgt
     9301 accactggtg gtagaccgcc acgttgatcc gcaggcgctt gtcgaacagg tcgaccttga
     9361 cgccgccttc gacgtcgtag accgtctccg gcttgaacgg cacgaagttg atcggcgcga
     9421 gtgagcgcag gttccacccg ccggaacgat agcctctccg gtgcgcaagg tagagcaggg
     9481 tgtcatcgtt cggcttgtag tcgaggctca gcgtccaggt cggcgaattg aactgggtcg
     9541 aaacctcctt ggtgcaggga ttgagcttcg cgccattggc atcggtcagg cggcaggtcg
     9601 gcggattgcc gatcttgctg cgggcgttga gatagcgggt atcgtgcgtg tagcgcccac
     9661 cagccgtgag ggtgagctga tcggttagct tgtagccgcc ctggaggtag cccgaaatcg
     9721 agtagttgcg agcgaacccg tcgttgatcc ggtcaccgcc gaggaacgac ttctgcacgt
     9781 cgtcacccga ttcgcggaaa tagtagagcc cgccgatcca gttgagccgc ttgtcgggag
     9841 tggtcccgct cagttgcagt tcgttggtga actgatcggc gttgagatcg ttggcggtgt
     9901 ggaacaccgt ggtcgggccg ccgtcgaagt cgagcacgac gttcgagtgg acatggcgga
     9961 agcccacgat gttcttgagg gtgacctcat cggaaagcgg gattttggtg gtgttgatca
    10021 actggtagct gtcaacgaag gccgaaggtg cccaatcgtt ctcgatgatg tggatcgaac
    10081 gggcctgctg gcgcgccagg gcttcgaagg tacccgccgt cttgcccgcg gtcgaaccgg
    10141 cccgcactgc ggtgagcgca aagccgttgc cggcatcatt ctcgtgaaag taggtgccga
    10201 tggtctcgtt cgagatgccg ttgccgggct cccacaggat cgagagccgc gcgctttcgg
    10261 tacgctcgtc atcagtgcgg gtgttggtgg tgagattgag gttatagccg tcgcgccggg
    10321 taatctggcc ggcggcgcgc acggccagcg tgctgctcag aggcacgttg agcaccacgg
    10381 tgccgcgccg cagattgtag tcaccgatcg aaaccgccgc cgaggcgccc agctcgaaag
    10441 tcggtttgtt gggcacgaac agcaccgcgc cgccggtggt attgcggccg aacagggtgc
    10501 cctgcgggcc cttgagcacc tgcaccgaag cgaggtcgta gaccgccgaa ttggtgccgt
    10561 gcggccgctg ctggacgacg tcggcgaaat agacgccaac tgagggatcc tgcgtgatca
    10621 gctgctcgag ctggcgctgg ccgcggatcg tgtagcccgg gacaaccgac ccgaagggcg
    10681 acggcgccac ctgaagcgac gggaccgaat agcgcagatc ctccgtggtc ttgatcccgc
    10741 gctggtcgat cgccttggcg ctgatggcgg taaccgcgac gggaacatcc tggaggcgtt
    10801 cctcccgccg ccgggcggta accacaatgt catcgccggc ggccgccgcg gccggtgcct
    10861 gagcggactg gtccgcaccg gcggcatggg cagcctgggc gccaaacgcg agtgcgatcg
    10921 acgcgacctg gcacagcagg ttccttgatt tcatagcccc ctccaattgc attacttttg
    10981 cggccaaatt tttttcagca ctgcgaaccg tttccttgca ccagatgact taactccgca
    11041 atacaatgtc atgtcgatca caaatgtaat gtgattacgc cgcaaagctc cctgcataac
    11101 atgctctacc aagaataatt gggtgtaagt aagtcatcgc tgataatcta ttcggcaaca
    11161 gtcttcgcgg tttggcaacg ggtattaccc cagcccgcgc gccacgacgt agcaaccaac
    11221 cgtgatcacc agcgcggcga agccgaattc catcttccgc ttggcgccgg ccaggcgctt
    11281 gccaaacgca atgccggcaa gggccccggc cacgccgcca acgaccagca ggccgaccag
    11341 ctgccactcg acatagtcgg agagggcata ggaggtggcc gtcgtcaggc ccagggcaaa
    11401 gaccgcgacc agggatgtgc cgacggcaat gcgcagcggc atgcccgtgg ccatgatcag
    11461 cgcaggcacg atcaggaaac cgccgccgat gccgaagaag ccggcgagca ggcccacgcc
    11521 gaggccggag gggatcagcc ggg
//
LOCUS       DATNBV010000414         6667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2797030,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000414 DATNBV010000000
VERSION     DATNBV010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2797030"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1132)
                     /gene="secF"
                     /locus_tag="VN627_09880"
     CDS             complement(155..1132)
                     /gene="secF"
                     /locus_tag="VN627_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXD03605.1"
                     /translation="MKLLKLVPDHTNIHFLRWRVPFYVVSLMLMAASIALVATKGLNM
                     GVDFVGGQMIRVTFTQSPAAPVAALRGEVASLGYGEPIIQQFGKPNEVSIRMKLPEGS
                     DHDAGLADAMAKRITGDIKTRHTDARIDGVDSVSGKVSKELGWTAFQALGLAALAVAA
                     YIWLRFEWQFGVGALLSLVHDVTLTLGLFALTGLEFDLNIVAALLTLIGYSLNDTIVV
                     YDRIRENLKKFRKMPMPELLDLSVNETLARTIVTSLSMLITLVALLLLGPDVIFGFTA
                     AITFGIFVGTYSSIYMAAPILIWLKVNPKSFVPVESETDRQERLARERG"
     gene            complement(1146..2753)
                     /gene="secD"
                     /locus_tag="VN627_09885"
     CDS             complement(1146..2753)
                     /gene="secD"
                     /locus_tag="VN627_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXD03606.1"
                     /translation="MLDFPRWKQTFYWAIAILAMLSALPSLFAFSNLRWPSALPRPEV
                     NLGLDLAGGSHILLEANPDQVKRQRLEAMDENVRQKLKTEAPDVAVGDISTLNGVVSF
                     MVENPAQVDKAREAVLPLTRGVGLSGQRDWDIGVLDGNRIVLTPTQAGVDADVSRAMD
                     TATEVVRKRIDALGTREPTIIRQGAQRIVVQVPGLKDPAALKSLLGQTAKLEFKLVDS
                     SASPSDIAQGIVPPGSEIVPCQDARSCGGVPAIAVRRLGGIKGDQLTDARQSNNQQNN
                     EPIVNITFNSEGGAKFARLTTENTGKPFAIILDNKVISAPRINEPILGGSAQISGSFT
                     TESANQLAISLRSGALPVDLKVVEERTVGPDLGADSIKKGGIAMLVGTTALVMFILIT
                     YGRFGVYATCALTVNVLMILGIMAVANTTLTLPGIAGFVLTIGAAVDANVLINERIRE
                     ERHRGRRVVQAVEMGYKEASRAIFDANITNVIAAALMFFFGSGPVKGFAVVLVIGICT
                     SVFTAVTMTRMWVAGWLRRARPADLVL"
     gene            complement(2792..3121)
                     /gene="yajC"
                     /locus_tag="VN627_09890"
     CDS             complement(2792..3121)
                     /gene="yajC"
                     /locus_tag="VN627_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXD03607.1"
                     /translation="MSTASLAILSAAAAGSPPAWTSFIPFVGMGLVFWFFLLRPQMQQ
                     QKAQRAKIDGLKKGDQVVTAGGLIGRIVKVDDAHVELELAPNVKVRAVRSTIADVLQP
                     GGKPAND"
     gene            3303..3379
                     /locus_tag="VN627_09895"
     tRNA            3303..3379
                     /locus_tag="VN627_09895"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3337..3339,aa:Pro,seq:ggg)
     gene            3577..5715
                     /locus_tag="VN627_09900"
     CDS             3577..5715
                     /locus_tag="VN627_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXD03608.1"
                     /translation="MTAIGVVAIALLAGAAAGEDNSPAPMSAAANRGSLVYDGNCASC
                     HGANLTGSSHGPQLTGKPFADHWLGSPDSALQGFIKQMMPPGQPGSLSDDEYRDVTAF
                     LLARNGAGPSETAGAAATAAAAAPKNALAPSAEGDFKARAIAAYLATLPGFHNQTLAN
                     YRPVTEAMLNRPDDGDWLNWRRTRDGHGDSPLAAITTANVGSLRLAWSIAMPDGVNEP
                     TPLVHDGVMFLQAAGGKIQALDAANGDVLWNYRYQRPDGGRVEPAAMRNFAMFGTSLF
                     MATSDAALVAIDARSGKQIWRTQEADPEKAFTHTAGPIIAHGLVIGGMNGCEMFSGVP
                     CFVAAHDPETGKELWRTPVIAQPGDPNIKTWGKVDPRFRAGGDMWIAGSYDPVLDTFY
                     IGTAQAKPWVPASRGMTADDAALYTNSTLALDPRTGKMKWWFQHVPGESVDHDVVFER
                     VLVDAGGKQLVLTEGKDGILWKLDRVTGKYVDLTQTVFQNVYSKVDRNRGRVTYREDI
                     RKAGIGYRMATCPASFGGHDWLASAYDARQQALVIPLLQMCGSMTGTKVEMALGSGGN
                     GGVGASAPKREEIEMPGSDGNYGKLAAYDVNTLRELWSYQQKVPFTTAALTTAGGLVF
                     VGDADRWFRAFDARTGKVLWQTRLASAVHGFPISYAVNGHQYLAVAAGQMGAYQVVTG
                     QIGGIYHPANGNALYVFELPRADASGKVGG"
     gene            5844..6635
                     /locus_tag="VN627_09905"
     CDS             5844..6635
                     /locus_tag="VN627_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXD03609.1"
                     /translation="MSEQPIIRNEWHGVLELVFNRPGKLNAINVEMMEILTQAVTDLR
                     DDDRHRVMLIRANGRYFSAGADVSAAQAFDIGNSPARLRNFARRHLHTGMSSLWDEME
                     RLEKPIVVAHHATCVGGGLEMSLSCDFRLAAQSAAYSFPELEFAILPLSGGISRLTRI
                     VGPHWARWLVLANEKVTADQALSMGLVHRVIPDETFEEEAKAFARKLAGHPAEAMAIG
                     KTTIELSALLGTADARAVERLTYGTLQFSSDYIEMQALRRAQHSK"
BASE COUNT         1166 a         2080 c         2322 g         1099 t
ORIGIN      
        1 catgctgaac ttgtttcagc atccatggcg tgccgctgcg gcaaaacctg gtgtcgggaa
       61 tggcacgctg cgctggcggc acccatgcgc catggatcct gaaacgagtt caggatgacg
      121 ggacatgctg gcggcgggcg accgccgtgc ccgcttaccc ccgttcgcgg gcgaggcgtt
      181 cctggcggtc ggtttcgctt tccacgggca cgaagctctt cgggttgacc ttgagccaga
      241 tcaggatcgg cgcggccatg tagatcgagg agtaggtgcc gacgaagatg ccgaaggtga
      301 tcgccgcggt gaagccgaag atcacgtccg ggccgagcag cagcagggcg acgagggtga
      361 tcagcatcga cagcgaggtg acgatggtgc gcgcgagcgt ttcgttgacc gagagatcga
      421 gcagctcggg catcggcatc ttgcggaatt ttttcagatt ctcgcggatg cggtcgtaga
      481 ccacgatggt gtcgttgagc gaatagccga tcagggtcag cagagcggca acgatgttga
      541 ggtcgaactc caaccctgtc agcgcgaaca ggcccagggt cagcgtcacg tcgtggacga
      601 ggctgagcag ggcaccgacg ccgaactgcc attcgaagcg cagccagata taggcggcga
      661 ccgccagggc ggcgaggccg agggcctgga acgcggtcca ccccagttcc ttggagacct
      721 tgcccgagac cgaatcgacg ccgtcgatcc gcgcatcggt gtggcgggtc ttgatatcgc
      781 cggtaatgcg cttggccatg gcatcggcga gaccggcgtc atggtcggaa ccttccggca
      841 gcttcatccg gatcgagact tcgttgggct tgccgaactg ctggatgatc ggctcgccat
      901 agcccaggct cgccacctcg ccgcgcaggg cggcgaccgg ggcggcgggg gactgggtga
      961 aggtgacgcg gatcatctgg ccgccgacaa agtcgacacc catgttgagg cccttggtcg
     1021 cgaccagcgc gatcgacgcg gccatcagca tcaggctgac cacgtagaac ggcacccgcc
     1081 agcgcaggaa gtggatgttg gtatggtcgg ggacgagctt cagcagtttc atggcgcgct
     1141 ctcccttaca gcacgaggtc ggccgggcgg gcgcggcgca gccagccggc gacccacatc
     1201 cgggtcatgg tcaccgcggt gaagaccgag gtgcagatgc cgatcaccag cacgacggcg
     1261 aagcccttca ccggacccga gccgaagaag aacatcagcg cggcggcgat gacgttggtg
     1321 atgttggcat cgaaaatggc gcggctcgct tccttgtagc ccatttccac cgcctggacg
     1381 acgcgccggc cccggtggcg ttcctcgcgg atgcgctcgt tgatcagcac gttggcatcg
     1441 accgcggcgc cgatggtcag cacgaagccg gcgatgccgg gcagggtcag cgtggtgttg
     1501 gccacggcca tgatgccgag gatcatcagc acgttcacgg tcagcgcgca ggtcgcgtaa
     1561 acgccgaagc ggccgtaggt gatgaggatg aacatgacca gcgcggtggt gcccaccagc
     1621 atggcgatgc cgcccttctt gatcgaatcg gcgccgagat cggggccaac cgtgcgctcc
     1681 tcgacgacct tgagatcgac cggcagggcg cccgagcgca gcgagatcgc cagctggttg
     1741 gcgctttcgg tagtgaagga gcccgaaatc tgggcgctgc cgccgaggat cggctcattg
     1801 atgcgggggg ccgagatcac cttgttatcg aggatgatcg cgaagggctt gccggtattt
     1861 tccgtggtca accgcgcgaa tttggcgccg ccttcggaat tgaaggtgat gttgacgatc
     1921 ggttcgttgt tctgctggtt gttcgactgg cgcgcgtcgg tcagctgatc gcccttgatg
     1981 ccgccgaggc ggcgcacggc gatggcgggg acgccgccgc atgagcgcgc atcctggcag
     2041 gggacgatct cgctgcccgg cgggacgatg ccctgggcaa tgtcggacgg gctggccgaa
     2101 ctgtcgacca gcttgaactc gagcttcgcg gtctggccga gcaggctctt gagcgcggcg
     2161 ggatccttga ggccggggac ctgaacgacg atgcgctggg cgccctggcg gatgatggtc
     2221 ggctcgcggg tgccgagggc gtcgatacgc ttgcggacga cttcggtggc cgtgtccatc
     2281 gcccggctga cgtcggcatc gacgccggcc tgagtggggg tgaggacgat gcggttgccg
     2341 tcaagcacgc cgatgtccca atcgcgctgg ccgctgaggc cgacgccgcg ggtgagcggg
     2401 aggacggctt cacgggcctt gtcgacctgg gcggggtttt ccaccatgaa gctgaccacg
     2461 ccgttgagcg tggaaatatc gccgacggca acgtctggcg cttccgtctt cagcttctgg
     2521 cggacgtttt catccatcgc ttccagccgc tggcgcttga cctggtcggg gttggcctcg
     2581 agcaggatgt ggctgccgcc ggcaaggtcg agcccgaggt tgacctcggg ccgcggcagg
     2641 gcggaaggcc agcgcaggtt ggagaaggca aacagcgaag gcagcgccga gagcatcgcc
     2701 aggatggcaa tggcccagta gaacgtctgc ttccagcggg gaaaatcgag catgtggggg
     2761 cgatcctcgc cttaaatcgt caggtgccgg ctcagtcgtt cgcgggcttg ccgcccggct
     2821 ggagcacatc ggcaatggtc gagcgcacgg cgcgaacctt gacgttgggg gccagctcca
     2881 gttcgacatg ggcatcgtca accttgacga tccggccgat caggccaccc gcggtcacca
     2941 cctggtcacc cttcttcagc ccgtcgatct tggcgcgctg ggccttctgc tgctgcatct
     3001 gcgggcgcag caggaagaac cagaagacca gacccatgcc gacgaaggga atgaacgagg
     3061 tccaggccgg cggggagccg gcagcggcgg cggagaggat ggcaagggag gcggtgctca
     3121 tgggcgcaat acggctttcg tcgcgagttt gggcccactg ggccgatctt gcgcggcggt
     3181 tagcagcaat gccggccagt ggcaacgcgc agccctcccc cgccgcctgc gctttcccaa
     3241 gcacggggca cagcacggcg cttgcatcgg cgcaaactgc tgcctatagg cgccgctccg
     3301 gtcgggacgt agcgcagcct ggtagcgcat cacactgggg gtgtgggggt cggaggttcg
     3361 aatcctctcg tcccgaccaa ttgtttctcc gcccggccaa gcgcccgcgg gcctctccaa
     3421 acagacgcac gccatctcca ccgcgatagc atcgtgtggc gctgttgact ttgcctcgcc
     3481 cgctcaccag cccttgcgca tagagcgcaa acagtgacca cgggcagagg caggcatttg
     3541 atgggacagg gatctggccg gcgtttcgcc ggaggcatga cggcgatcgg cgtggtggcg
     3601 atcgccctgc tggctggcgc ggcggcaggc gaggacaaca gccctgcccc gatgagcgcg
     3661 gcggccaatc gcggcagcct ggtctacgac ggcaactgcg ctagctgtca cggcgccaac
     3721 ctgaccggct cgtcgcacgg cccgcagttg acgggcaagc cctttgccga tcactggctc
     3781 ggcagcccgg acagtgcgct ccagggcttt atcaaacaga tgatgcctcc gggacagccc
     3841 ggttcgctca gcgacgacga ataccgcgac gtgacggcct tcctgctggc ccgcaacggg
     3901 gccggcccaa gcgaaacggc cggggcggcg gcgaccgcgg cggcggcggc gccgaaaaac
     3961 gcgctcgcgc cgagcgccga gggtgacttc aaggcccggg cgatcgcggc ttatctggcc
     4021 acgctcccgg gctttcataa ccagacactg gccaattacc gcccggtgac cgaagcgatg
     4081 ctcaaccggc ccgatgacgg ggactggctg aattggcggc gtacccgtga cggccatggc
     4141 gacagtccgc tggccgcgat caccaccgcc aatgtcggtt ccctaaggtt ggcctggtcg
     4201 atcgccatgc cggacggggt caacgagccg acgccgctgg tgcacgacgg ggtgatgttc
     4261 ctccaggccg cgggcgggaa gatccaggca ctggatgcgg ccaatggcga tgtgctgtgg
     4321 aactaccgct accagcgccc cgacggcggg cgggtggaac cggcggccat gcgcaatttt
     4381 gccatgttcg gcaccagcct gttcatggcc acgtcggatg ccgccctcgt ggccatcgac
     4441 gcgcgcagcg ggaagcaaat ctggcgcacg caggaggcgg accccgaaaa ggcgtttacc
     4501 catacggcgg ggccgatcat cgctcacggg ctggtgatcg gcgggatgaa cgggtgcgag
     4561 atgttcagcg gcgtgccctg ctttgtggcc gcccacgatc cggagaccgg caaggaattg
     4621 tggcgcacgc cggtgatcgc ccagccgggc gacccgaaca tcaagacctg gggcaaggtc
     4681 gatccccggt tccgcgcggg aggcgacatg tggatcgccg gcagctacga tcccgtgctc
     4741 gacacgttct acatcggcac ggcccaggcc aagccgtggg ttccggcgag ccgcgggatg
     4801 accgcggacg acgcggcgct ctatacgaat tcgaccctcg cgctcgatcc ccgcacgggg
     4861 aagatgaagt ggtggttcca gcacgtgccg ggcgaatcgg tcgaccatga cgtggtcttc
     4921 gagcgggttc tggtcgatgc cggcgggaag cagctggtgc tgaccgaagg caaggacggc
     4981 atcctgtgga agctggaccg ggtgacgggc aaatacgtcg acctcaccca gaccgtgttc
     5041 cagaacgtct attcgaaggt ggaccgcaat cggggccggg tgacctaccg cgaggacatc
     5101 cgcaaggccg ggatcggcta tcgcatggca acctgtccgg ccagcttcgg cggccatgac
     5161 tggctcgcct cggcctatga cgcgcgccag caggcgctgg tcatcccgct gctccagatg
     5221 tgcggcagca tgaccggaac caaggttgag atggcgctgg gcagcggagg caatggcgga
     5281 gtgggtgcca gcgcgccaaa gcgcgaggaa atcgagatgc ccggctctga cggcaactac
     5341 ggcaagctgg cggcctatga cgtgaacacg ctgcgcgagc tgtggagcta ccagcagaag
     5401 gtgccgttca cgaccgcggc cctgacgacg gccggtggcc tggtgttcgt gggcgatgcc
     5461 gaccgctggt tccgggcttt tgatgcgcgc accggcaagg tgctgtggca gacgcggctg
     5521 gccagtgccg ttcacggatt ccccatttcc tatgcggtca acgggcacca gtatctggca
     5581 gtcgccgccg ggcagatggg ggcctatcag gtggtaaccg gccagatcgg cggcatctac
     5641 cacccggcca acggcaatgc gctgtatgtc ttcgaactgc cccgggccga cgcgtccggc
     5701 aaggttggcg gctgatcgtt gccgcccacc atatgagaga tatcttggcg ccttacatta
     5761 agggactggc gccaggggca ctgccccggc agtaagccgc aatctgcgat cgcggtaagc
     5821 cgcgatctca cgggaggatc agagtgagcg agcaaccgat tatccgcaac gaatggcacg
     5881 gcgtgctgga gctggtgttc aaccggcccg gaaagctcaa tgccatcaat gtcgaaatga
     5941 tggagatcct cacccaggcg gtgaccgacc tgcgcgacga cgatcgccac cgggtcatgc
     6001 tgatccgtgc caatggccgc tatttctcgg ccggcgccga cgtctcggcc gcgcaggcct
     6061 tcgacatcgg caacagcccg gcgcgtctgc gcaacttcgc ccggcgccac ctgcacaccg
     6121 gcatgtcgag cctgtgggac gaaatggaac ggctggaaaa gccgatcgtc gtcgcccatc
     6181 acgcgacctg cgtgggcggc ggcctggaga tgtcgctctc gtgcgatttc cgcctggctg
     6241 cgcagagcgc cgcctattcg tttcccgagc tggaattcgc catactgccg ctgtcaggcg
     6301 ggatcagccg cttgacccgg atcgtcggcc cgcactgggc gcgttggctc gtgctcgcca
     6361 atgagaaagt gacggccgac caggccctgt cgatgggcct ggtccaccgg gtgattcccg
     6421 acgaaacctt cgaggaggag gcaaaggcct tcgcccgcaa gcttgccggt catccggccg
     6481 aggccatggc catcggcaag accacgatcg agctgtcggc cctgctcggc acggcggatg
     6541 cccgtgcggt cgagcggttg acctatggga cgctgcaatt ctcgtccgac tatatcgaga
     6601 tgcaggcgct aaggcgcgcg cagcacagca aatagcgggg attttcctac attcgccaga
     6661 ttggtta
//
LOCUS       DATNBV010000415         2682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3738256,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000415 DATNBV010000000
VERSION     DATNBV010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3738256"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VN627_09910"
     CDS             complement(<1..776)
                     /locus_tag="VN627_09910"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HXD03610.1"
                     /translation="MKLADDAPIVAVPMCVKQIDGQNFHTVGEKYLTALIEGAGAYPL
                     SFPALGAVLDPAALLDQVDGVLFTGSPSNVAVHYYEGAPDRPDSPQDPGRDAITLPLI
                     RAAIERDVPVLCICRGFQELNVALGGTLDTHVHLAEGRMDHRAPRDVPYEEMYGPRHK
                     VTFLAGGQFARILGKAETQVNSLHWQGIAKPAARIQVEGRAEDGVIEAVSLKDHAGFC
                     LGAQWHPEFHVMENPDSMALFKAFGAATRARRQQRLSGGK"
     gene            1012..1833
                     /locus_tag="VN627_09915"
     CDS             1012..1833
                     /locus_tag="VN627_09915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HXD03611.1"
                     /translation="MGEFGESLLQELLVAKAAIVPGWIVLALVIEQWRPAAPAPIALA
                     GYGAAWFARWGRNLGLFALNSALSTLFVLPVTVWAALHAPWSRAELVGSAVPAWVWLP
                     VDIVLLDFWIYWWHRANHEWRFLWRFHEVHHRDRHLDATTALRFHFGEVALSTAVRAI
                     VIIVLAIPLTSVILFEILVMVATVFHHANWRIPRGLETRLARIIITPSRHWVHHHRVQ
                     ADTDSTYGTIFSFWDRWVGTTTPTERTVLMPLGVEGKEELPMIALIGRPTGVRDQ"
     gene            complement(1842..2609)
                     /locus_tag="VN627_09920"
     CDS             complement(1842..2609)
                     /locus_tag="VN627_09920"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HXD03612.1"
                     /translation="MARAVAMGHATNYMSCRCSSRIGSQALDRMKIFKPQLPQFHRRA
                     LLRAGAGLAGAAALGLALPRIAFAETAQEAQLTDADKGDVARIEGYLNGLTTVKASFQ
                     QFTQNEGLAFGRIYLRRPGRLRVEYDPPSEILLVADGMLLSYYDAELNHIEQVPLKLS
                     PMWFLLRENVKLGGDVTVTSFKKAADSILIGIVQSDEPDAGSVMLELGDKPLELRQWT
                     ITDSAGTQVRVGLYNAEFGMPLSGALFATPRKQDRNR"
BASE COUNT          455 a          882 c          851 g          494 t
ORIGIN      
        1 ttcttcccgc cgctcagccg ctgctgccgg cgcgcgcgcg tggctgcgcc gaacgccttg
       61 aaaagcgcca tggaatcagg gttctccatg acatggaact ccggatgcca ctgcgcaccc
      121 aggcaaaagc cggcatgatc cttgaggctc accgcctcga taaccccatc ctcggcgcgg
      181 ccttccactt ggatgcgcgc tgccggtttc gcgatccctt gccaatgcag cgaattcacc
      241 tgggtctcgg ccttgccgag gatgcgcgcg aactggccgc cggcgaggaa cgtgaccttg
      301 tgtcgcgggc cgtacatctc ttcgtacggc acgtctcgcg gcgcgcgatg gtccatccgc
      361 ccttcggcca ggtggacatg ggtgtcgagc gtgccgccca gcgcgacatt cagctcctgg
      421 aaaccgcggc agatgcagag caccggcaca tcgcgctcga tcgccgcgcg gatcagcggc
      481 agcgtgatgg catcgcgccc gggatcctgc gggctgtccg gccggtcggg ggcgccttcg
      541 tagtaatgta ccgcgacatt cgacgggctg ccggtgaaaa ggaccccgtc cacctggtcc
      601 aacaaggccg ccgggtccag caccgctccc aaagcgggaa aggacagcgg ataggcgccc
      661 gccccctcaa taagggctgt caggtatttc tcgccgaccg tatggaaatt ctgtccgtcg
      721 atctgcttca cgcacatcgg aacggccacg atcggcgcgt catcggcaag cttcatgtcg
      781 gctccggccc agatcccacc aaggtcttga tcgggggata ttaaggaaat ctactcgaat
      841 ctagggccgg cccagccccc cgacaaacgc agcccgcgca gcccccggtt tcgcgggatg
      901 cagggcgatt ggacgacttt ccatccaacc cttcacccag gtacggggaa accggaatag
      961 ctgaggctac caccccttct cagcgccccc ggaaccggga atattgaccg gatgggcgag
     1021 ttcggggagt cgctgttgca ggagctgttg gtcgccaagg cggcgatcgt gcccggctgg
     1081 atcgtgctgg ccctggtgat cgagcaatgg cgcccggccg cgccggcgcc gatcgccctg
     1141 gccggctatg gtgcggcctg gttcgcccgc tgggggcgca atctcggcct gttcgccctc
     1201 aacagcgcgc tgtcgaccct gttcgtcctg ccggtcacgg tctgggcggc gcttcacgcc
     1261 ccgtggagcc gggccgagct ggttgggtcg gcggtgccgg cctgggtctg gctgccggtc
     1321 gacatcgtgc tgctcgattt ctggatctat tggtggcacc gcgccaacca cgaatggagg
     1381 ttcctctggc gcttccacga ggtgcatcac cgcgaccggc atctcgacgc caccacggcg
     1441 ctgcggtttc atttcgggga ggtcgcgttg tcgacggcgg tgcgggcgat cgtgatcatc
     1501 gtactcgcca ttcccctgac ctcggtcatc ctgttcgaga tcctggtgat ggtggcgacg
     1561 gtctttcatc atgccaactg gcgcatcccg cgggggctcg aaacgcggct cgcgcgcatc
     1621 atcatcacac cgtcgcgcca ctgggtgcat caccaccgcg tgcaggccga taccgattcc
     1681 acctacggca cgatcttcag cttctgggac cgatgggtcg gcaccaccac gccgaccgag
     1741 cgcacggtcc tgatgccgct tggcgtcgag gggaaggaag agttgccgat gatcgcgctg
     1801 atcggaaggc cgacgggcgt ccgcgatcaa taatcagcgt gttacctgtt tcggtcctgt
     1861 ttgcgcggcg tggcgaacag agcgccgctg agcggcatgc cgaactccgc gttatagagg
     1921 ccgacgcgca cctgggtgcc ggccgaatcg gtgatggtcc attgccgcag ctccagcggc
     1981 ttgtcgccaa gctcgagcat gacagagccg gcgtcgggct catccgactg gacgatgccg
     2041 atcaggatcg agtccgccgc cttcttgaag gaggtgaccg tgacgtcgcc acccagcttc
     2101 acattctcgc gcagcaggaa ccacatcggc gacagcttca gcggcacctg ctcgatgtga
     2161 ttgagctcgg catcgtaata ggaaagcagc atgccatccg cgaccagcag gatctccgac
     2221 ggcggatcat attcgaccct gaggcgcccc ggccggcgca gatagatacg cccgaaggcc
     2281 aggccctcat tctgggtgaa ttgctggaag ctcgccttca ccgtggtcag gccgttgaga
     2341 tagccctcga tgcgcgcgac atcgcccttg tcggcatcgg tcagttgcgc ttcctgcgct
     2401 gtttccgcga acgcgatgcg cggcaaggcg aggccgaggg cagctgcgcc agcgaggccg
     2461 gcaccggccc gcagcaaggc gcggcggtgg aattgaggga gctgtggctt gaaaatcttc
     2521 atgcgatcca acgcttgcga tccgatccga gatgaacaac ggcaagacat atagtttgtt
     2581 gcgtgaccca ttgccacggc ccgcgccatc acgttcccgc aacttggcag ttatcttcta
     2641 atggattgaa attacgccgt ttctatgacc ggccgaggcc ct
//
LOCUS       DATNBV010000416         3559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_253469,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000416 DATNBV010000000
VERSION     DATNBV010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253469"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VN627_09925"
     CDS             complement(<1..622)
                     /locus_tag="VN627_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03613.1"
                     /translation="MNAKLHWPDERHPHAGPGKGKRARMVDHPLFPAILVLWFAAPLA
                     FIALALPASLLEHAAAAIRLDALVPAARPPLGFTARALLAFALALGGGLGGWLTALVL
                     RRRTLASVDHADALPMEAKAETVAPLVSSPVDEAHDDDGDDLARLAAARANLPQRRRA
                     LTSETAEAPGILEISAIPALEPLGAGPAAVEPIAAEAAQPAWAPAPV"
     gene            complement(651..1871)
                     /locus_tag="VN627_09930"
     CDS             complement(651..1871)
                     /locus_tag="VN627_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04063 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HXD03614.1"
                     /translation="MRVLHVLDHSLPLHSGYTFRTRAILKAQEGLGLEVRGITGQRHA
                     ANGGSAEGALELAEGLAFHRARGLPAGPPGLREWREIAALASAIERLAGDWRPDVLHA
                     HSPALCGMAALIAGRRLGLPVVYEIRAFWEDAAVGNGQGREGSLKYRLIRALENRVVA
                     RADAVVTICAGLRDDLVGRGIAPERITLSPNGVDLTLFGAPPPPSPALGARLGLDGDG
                     PVIGFIGSFYDYEGLDVLIDAMPLLLARERGARLLLVGGGPMDAALRARAAASPAAGM
                     IHFAGRVPHAEVERWYALMDIMAYPRKASRLTELVTPLKPLEAMAQGKLVAASAVGGH
                     RELVTHGATGMLFQADDPAACAATLASLAGNRQDWEALRERGRAHVRANHDWAVNARR
                     YLPVYQAALDKRPL"
     gene            2059..3420
                     /locus_tag="VN627_09935"
     CDS             2059..3420
                     /locus_tag="VN627_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5935 domain-containing protein"
                     /protein_id="HXD03615.1"
                     /translation="MVDLGLFLFMAALLVLSLKRPFLAVLAYLYVDIVSPQKISWHLL
                     ASIPISLITFLAAFGLWLVADDKADSRFTLRQGLLLALLAYCGASTMFADFPAEAAEK
                     WSWVWKALLFATFLPLTLRTRLRIEAAALFMVLSAGSIVITGGIKTALTGGGYNALHF
                     FVQDNAGLYEGSIISCVAIAIIPLILWLARHGTIYPKGRLVNLFAGALIFACLLIPVG
                     TQARTGLLCIALLGGLMLRTVRRRFLYLGGAAALALAAVPLLPTSFTARMSTIGNHEA
                     DQSASTRIAVWQWTLGYVKDHPLGGGFYAYLSNRLVIETRDAETSGSTTAIATHTVTD
                     QARAFHSSYFEMLGEQGWPGLGLWLWLQLSGLWQMERLRRHWRKRAEAEGAGPDCQWQ
                     APLANALQQAQLIYLLGAAFVGIAFQPFILMLIGLQCGLWTYLKRVGEPRPLARPLRP
                     AVA"
BASE COUNT          529 a         1166 c         1271 g          593 t
ORIGIN      
        1 aaaccggtgc cggggcccat gcgggctgcg ctgcttccgc cgctatcggt tcgacagcgg
       61 ccggcccggc gccgagtggt tcaagggccg ggattgcgga gatttcaaga atgccgggcg
      121 cttcggcggt ctcgctggtg agggcgcggc ggcgctgggg caggttcgcg cgggcggcgg
      181 ccagccgggc gagatcgtcg ccatcgtcgt cgtgcgcctc atccacgggc gaggaaacca
      241 gcggcgccac ggtctcggcc tttgcctcca tcggcagcgc atcggcgtga tcgacgctgg
      301 cgagtgtgcg ccgccgcagg accagtgccg tcagccagcc gcccaagccg cccccgagcg
      361 ccagcgcgaa tgccagcagg gcgcgggcgg tgaagccgag cgggggccgg gccgcgggca
      421 ccagcgcgtc gaggcggatt gccgcggcgg cgtgttcgag gaggcttgcg ggaagggcaa
      481 gggcgataaa ggcgagaggg gcggcgaacc acagcaccag aatggcggga aacagcggat
      541 ggtcgaccat ccgcgcgcgc ttgcccttgc ccgggccggc gtgcggatgc ctttcatctg
      601 gccagtgcag cttggcgttc atcggcgaaa ccacggtccc tgttgcccca tcacaatggc
      661 cgtttgtcta gcgcggcttg gtaaacaggg agataacgac gcgcattgac ggcccagtca
      721 tggttggcgc ggacgtgggc ccggccccgt tcgcgcaggg cctcccagtc ctggcggttt
      781 ccggcgaggc tcgcgagcgt ggcggcgcaa gcggctggat catcggcctg gaagagcatg
      841 ccggttgccc catgggtcac cagttcacgg tgcccgccga cggcggaggc ggcgaccagc
      901 ttgccctggg ccatggcctc caggggtttc agcggggtga cgagttcggt caggcggctg
      961 gccttgcggg ggtaggccat gatgtccatc agcgcatacc agcgctcgac ctccgcatgt
     1021 ggcacgcgcc cggcgaagtg gatcatgccg gctgccggcg aggcggcggc cctagcccgc
     1081 agcgctgcgt ccatcggccc gccgcccacc agcagcagcc gcgcaccgcg ttcacgcgcc
     1141 agcagcagcg gcatggcgtc gatcaacacg tcgagcccct cgtaatcgta gaagctgccg
     1201 atgaagccga tcaccggccc gtcgccgtca aggccaagcc tggcacccag ggccggggag
     1261 ggcggcggcg gcgcgccgaa cagggtcagg tccaccccgt ttggcgagag ggtgatccgc
     1321 tccggggcga tgccgcgccc gacgagatcg tcgcgcagac cggcgcagat cgtaaccacg
     1381 gcatcggcgc gggcgacgac cctgttctcc agcgcgcgga tcaggcgata cttgagcgag
     1441 ccttcgcggc cctgcccatt gccgaccgcc gcatcctccc agaaggcgcg gatttcgtag
     1501 accaccggca ggccaagacg gcgcccggcg atcagcgcgg ccatgccgca cagcgcgggc
     1561 gagtgggcat ggagaacgtc gggccgccag tcaccggcga gccgttcgat cgcgctggca
     1621 agggccgcga tctcgcgcca ttcgcgaagg ccgggcgggc cggcgggcag cccgcgggca
     1681 cggtggaacg ccaggccctc ggccagttcg agtgcaccct cggccgagcc gccattggcg
     1741 gcgtggcgct ggccggtgat cccgcgcacc tccagtccca gcccttcctg cgccttgagg
     1801 atggcccggg tgcggaaggt gtagccgctg tggagcggca gggaatggtc gaggacatgg
     1861 aggacgcgca tggctccgtg ggcctagcgc aagaggctta acgcgccgtc aaccgcgatc
     1921 ggataggttc gtccggatgg tcgatctgtt cgctctcatg ctgactcacg gactgctggc
     1981 actggcggct tggaggctgg tgctgcgtgg cgatctggat caggattccc ggcgggaccc
     2041 gccccatggc tgatccccat ggttgatctg gggctattcc tcttcatggc ggcgctgctg
     2101 gtgctgtcgc tcaagcggcc gttcctggcc gtgctggctt acctgtacgt ggacatagtc
     2161 tcgccgcaga agatctcgtg gcacctgctg gcctcgatcc cgatctcgct gatcactttc
     2221 cttgccgcct tcggcctgtg gctggtggcc gacgacaagg ccgacagccg gttcaccctg
     2281 cgccagggcc tgctgctggc gctgctcgcc tactgcgggg cgagcaccat gttcgccgat
     2341 ttcccggcag aggcagcgga aaagtggtcc tgggtgtgga aagcgctgct cttcgcgacc
     2401 ttcctgccgc tgaccctgcg cacgcgcctg cggatcgagg cggcggcgct gttcatggtg
     2461 ctttcggccg gttccatcgt catcaccggg gggatcaaga cggcgctgac cggcggtggc
     2521 tacaatgccc tgcacttttt cgtgcaggac aatgccgggc tatatgaagg gtcgatcatt
     2581 tcctgcgtgg cgatcgcgat cattccgctg atcctgtggc tcgcgcgcca tggtacgatc
     2641 tatccgaagg gacggctggt gaacctgttc gctggggcgc tgatctttgc ctgcctgctt
     2701 attccggtgg gcacccaggc gcgaaccggt ctcctgtgta tcgccctgct cggtgggttg
     2761 atgttgcgca cggtgcgccg ccggttcctt tatctgggcg gagcagccgc gctggcgctg
     2821 gccgcggttc ccctgctgcc aaccagcttc accgcgcgaa tgagtaccat cggcaaccac
     2881 gaggccgacc aatccgcttc gacccgcatc gccgtatggc agtggacgct cggctatgtg
     2941 aaggaccacc cgcttggtgg cgggttctac gcctacctct cgaaccggct ggtgatcgag
     3001 acgcgcgacg ccgagaccag cggaagcacc acggccatcg caacccatac ggtgaccgat
     3061 caggcgcggg ctttccattc cagctatttc gagatgctgg gcgagcaggg ctggccgggg
     3121 ctggggctgt ggctgtggct gcaattgtcc ggactgtggc aaatggagcg gctgcgacgg
     3181 cactggcgaa aacgcgcgga ggccgagggc gctggacctg actgccagtg gcaggcgccg
     3241 ctggccaatg ccttgcagca ggcgcaattg atctacctgc tgggcgccgc cttcgttgga
     3301 attgccttcc agccgttcat cctgatgctg attggcctgc aatgcgggct gtggacctac
     3361 ctgaaacggg tgggcgagcc gcgccccttg gcgaggccgc tgcgcccggc ggttgcctga
     3421 cgcgacgatc gcggccaagc tcgcccggca agccaagatt tccgggagag cacatgagac
     3481 tgggagaact gccggaccac gtcggcatga cgctgatctc gccgtgggtg gtggtcgacc
     3541 aggcgcggat cgacgcctt
//
LOCUS       DATNBV010000417         3984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_871920,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000417 DATNBV010000000
VERSION     DATNBV010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871920"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VN627_09940"
     CDS             <1..206
                     /locus_tag="VN627_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HXD03616.1"
                     /translation="EQPFVYRGFQMINQEALQDIRGDILRGWNQSGLLPLIYAHMFSL
                     ELIREIFGRQVQQGKGPVAAPAA"
     gene            complement(538..1530)
                     /locus_tag="VN627_09945"
     CDS             complement(538..1530)
                     /locus_tag="VN627_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HXD03617.1"
                     /translation="MEPAVTDEDSIFSQQLDGFVSSGGTIPGLAGTPAWVLRAREPSA
                     IPVLIAVPHAGRSYPPEVGERLRNPAHAALRLEDRMVDRLGEAVARLTGAALLVAQAP
                     RAMIDLNRAGDDVDWSMFARERGEALRPPSPPGGRAGGGLGIVPRRLPGVGELWKRPH
                     RREELEARLTHVHGPYHDELARQLARLHERWGAALLLDLHSMPPVVARGGAAPEFVVG
                     DRFGASCAGTLTALAFAWFARGGRAAAHNRPYAGGYALDRHASPRRGIHAFQLEIDRS
                     RYLDSQLLEPGVGFAAMAEHLAGLVRALAGEVAAMGRVAAASRRGPDTWAEAAE"
     gene            1677..2051
                     /locus_tag="VN627_09950"
     CDS             1677..2051
                     /locus_tag="VN627_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXD03618.1"
                     /translation="MIRILLAEDEDAMRTYLSRALENAGYAVAAVDRGTAAMPLLQSE
                     HFDLLLSDIVMPEMDGIELAQRCAEVSPATKVMFITGFAAVTLKASREAPQAKVLSKP
                     FHLKDLVMEVDRLFSAPGSLSI"
     gene            2195..2269
                     /locus_tag="VN627_09955"
     tRNA            2195..2269
                     /locus_tag="VN627_09955"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2227..2229,aa:Val,seq:gac)
     gene            2467..3786
                     /gene="chrA"
                     /locus_tag="VN627_09960"
     CDS             2467..3786
                     /gene="chrA"
                     /locus_tag="VN627_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HXD03619.1"
                     /translation="MTEPSQVQHPAFAELVRVSARIGCLSFGGPVGQIALMHRELVEE
                     RQWVSEEQYLHALNFCHLLPGPEAQQLAIWIGWKLHGLRGGLAAGLLFVLPGALVMLA
                     LSVLYGFAAHLEWFAALFLGIKAAVLAIVVQALLRVAGRALNTGFKRWVAALALAGLF
                     VFNLPFPVVVLGALLLGTLVAAWRPGWLALGQVAPPAPSSPRPWGASLRAIVAGGIAW
                     AAPLGLVLATLGPDHVLWRIGSFFARLAVVSFGGAYAVLAYMAQAAVQNYGWLGAGEM
                     ADGLGLAETTPGPLIMVTQFVGYIAAFRAPEPFTPLIAGVIGAALTTWVTFAPCFLWV
                     FALAPWIERLEHARRLKGGLATLTAAIVGVIANLSLWFALHVLFARVNTLALGPARLQ
                     VPELASLDWRALILAAVAALLVFRARWNVIKVLAVTAAGGVALGLLA"
     gene            complement(3778..>3984)
                     /locus_tag="VN627_09965"
     CDS             complement(3778..>3984)
                     /locus_tag="VN627_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03620.1"
                     /translation="CVFLNMPMMQQPEAYLGHVTDDSFDGEGCLKDGSLKEIVLKLST
                     AFADWVDMIVRSRRLIAEDAAHSR"
BASE COUNT          596 a         1348 c         1354 g          686 t
ORIGIN      
        1 tcgagcagcc gttcgtctat cgcggcttcc agatgatcaa tcaggaagcg ctgcaggaca
       61 ttcgcggcga catcctgcgc gggtggaacc agagcggcct gctcccgctg atctacgccc
      121 acatgttctc gctcgaactg attcgcgaaa ttttcggacg ccaggttcaa cagggcaagg
      181 gcccggttgc agcgccggcc gcctgatcca gttagggccg gccgcgcggc gggcggccct
      241 aactgattga aatggcaggt tcagtggagt tctgtcccat tgcccgcccg gccggggccc
      301 agattcggcg gttacgcaaa tcggatccag ctcaagccat ttgcggttga attttttctg
      361 gaccgcgcta aataggcaac actcgatcgg tgcttccctc atggcccggt cgggttggtg
      421 cacttgttgc acctcctccc tgaaccttgg ccacctcgtg cgattgcacg aggtggtttt
      481 ttatgggttt tcggcaagct tcggcaaaac acgggtctgg cgcggcgaaa accggcgcta
      541 ttcggcggct tccgcccagg tgtccgggcc ccggcgggag gcagcggcca ctcggcccat
      601 ggcagccact tccccggcca gcgcgcggac caggccggca agatgctcgg ccatggcggc
      661 aaaacccaca ccgggctcaa gcagctgcga atcgaggtag cgcgaccgat cgatctcgag
      721 ctggaaggcg tggatcccgc gccgcgggct ggcatggcga tcaagcgcat agcccccggc
      781 atagggccgg ttatgcgcgg ccgcccggcc gccccgggca aaccacgcga aagccagcgc
      841 ggtgagcgtt cccgcgcagc tggcgccgaa gcggtcgccc acgacgaatt ccggcgcagc
      901 gccgccacgg gcgacgaccg gcggcatcga atggagatca agcaaaagcg ccgcgcccca
      961 ccgctcgtgc aggcgggcca gctgccgcgc cagttcgtca tggtagggcc catggacgtg
     1021 ggtcaggcgc gcctcaagtt cctcgcgccg gtgcgggcgt ttccacagtt cgcccacgcc
     1081 cggcaggcgt cgcggcacga tgccgagccc gccgcccgcg cggccacccg gcgggctcgg
     1141 cgggcgcagg gcctcacccc gctcgcgggc gaacatcgac cagtccacgt cgtcgccggc
     1201 gcggttgaga tcgatcatcg cccgcggcgc ctgggcgacc agcagggcgg caccggtcaa
     1261 ccgggcgacc gcctcgccca gccgatcgac catgcggtct tccagccgga gcgcggcatg
     1321 cgccgggttg cgcaggcgct cccccacctc gggcggatag gagcgcccgg catggggcac
     1381 ggcgatcagc accgggatgg ccgagggctc ccgcgcgcga agcacccagg ccggcgtccc
     1441 ggccagcccg ggaatcgtcc caccgctgga gacgaagcca tcaagttgtt gggagaaaat
     1501 cgaatcctcg tcagtcaccg ccggctccat ggtgctaggc tggaccgccc ggccgcgcat
     1561 gtcaaagggg cttccgggcc gtcggacgac gatttcttaa gaggttgcag cctatcggat
     1621 tcgcccgggc cggtccggcg ccagccccta ttcgagaacc ccacaaaggc accttcatga
     1681 tccgcatact tctggccgaa gacgaagacg caatgcggac ctacctttcg cgggcactgg
     1741 agaacgcggg ctacgcggtg gcggccgtgg atcgcggcac ggcggccatg ccgctgctcc
     1801 agtccgagca cttcgacctg ctgctctccg atattgtcat gcccgaaatg gacgggatcg
     1861 aactggccca gcgctgcgcc gaggtgagcc cggcgaccaa ggtgatgttc atcaccggct
     1921 ttgccgcggt caccctcaag gccagccgcg aggcgcccca ggccaaggtc ctgtccaagc
     1981 cgtttcatct caaggacctg gtgatggaag tcgaccgcct gttcagcgcc ccgggcagcc
     2041 tgtctatctg atcggctgcg gaaagcgcct ccgcgcttcc ctggcggcac aagcccgttc
     2101 ctttccagga ttcctcaaaa cactccttga tttgcccgaa cctgcccgtt aaaggcgccc
     2161 tcctgcccgg catgccgggc tgctcgaatg gatcgggcgt atagctcagt ggtagagcac
     2221 tgtgttgaca tcgcaggggt cggaagttca atccttccta cgcccaccat tcaaaaaacc
     2281 ccggcagccg caaggttcgc cggggttttt tgcgcttgtc ggcgccaggc gtggcagccc
     2341 ggttgacgga tccccaccgg cggcataacc gggagccagc ggggcacagc aacctcccga
     2401 ccaggccgtt agcggcccaa gcgttgcctc attgtccgcg cctgcgcgga tggtagggct
     2461 tgtcgcatga ccgaaccttc ccaagtgcaa catcccgcat tcgccgaact cgtgcgtgtt
     2521 tccgcgcgga tcggctgcct gtccttcggg ggaccggtcg ggcagatcgc cctcatgcac
     2581 cgcgaactgg tggaggagcg ccagtgggtc agcgaggagc aatatctcca cgcgctgaac
     2641 ttctgtcacc tgctgcccgg gcccgaggcg cagcaactgg cgatctggat cggctggaag
     2701 cttcacggcc tcaggggcgg tctggcggcc gggctcctgt tcgttctccc gggcgcgctg
     2761 gtgatgctgg cgctctcggt gctctatggc tttgccgccc acctcgagtg gttcgccgcg
     2821 ctgttcctgg ggatcaaggc tgccgtgttg gcgattgtgg tccaggccct gctgcgggtg
     2881 gcgggccggg cgctcaacac cgggttcaaa cggtgggtgg ccgcactcgc gctggccggg
     2941 ctgttcgtgt tcaacctgcc ctttccggtg gtggtgctgg gtgcgctgct gctgggcaca
     3001 ctggtggcgg cgtggcggcc gggctggctg gcgctggggc aagtcgcccc gccggcgccg
     3061 agctcgcccc ggccgtgggg ggcaagcctg cgggctatag tggcgggcgg catcgcctgg
     3121 gccgcgcccc tcggtctggt gctggccacg cttggtcccg atcacgtgct gtggcggatc
     3181 ggcagcttct tcgcgcgcct cgccgtggtc agcttcggcg gggcctatgc ggtgctcgcc
     3241 tacatggccc aggcggcggt gcagaactac ggctggctcg gcgccggcga aatggccgac
     3301 gggctgggcc tcgccgagac cactccgggg ccgctgatca tggtgacgca gttcgtcggc
     3361 tatatcgccg cgttccgggc cccggagccg ttcacgccgc tgatcgccgg cgtgattggc
     3421 gcggcgctga ccacgtgggt gacctttgcg ccctgcttcc tgtgggtctt cgccctcgcg
     3481 ccatggatcg agcggctgga acatgcccgg cggctcaagg gtggcctcgc cacgctgaca
     3541 gcggcgatcg tcggcgtgat tgccaacctg tcgctgtggt tcgcgctgca cgtactgttc
     3601 gcgcgagtga acacgctggc gctcggcccg gcccggctgc aggtgccgga gcttgccagc
     3661 ctcgactggc gcgcgctgat cctggcagcc gtcgcggcac tgctggtatt ccgcgcgcga
     3721 tggaacgtga tcaaggtcct cgccgtcacg gcagcggggg gggtagcgct gggtctgcta
     3781 gcgtgagtgc gccgcgtctt ccgcgatcag gcggcgcgag cggacgatca tgtcgaccca
     3841 gtcagcaaaa gccgtggaga gcttgaggac gatctccttg agcgagccgt ccttcaggca
     3901 gccttcgcca tcgaagctgt cgtcggtaac gtgcccgaga taggcctcgg gctgctgcat
     3961 catcggcatg ttgaggaaca cgca
//
LOCUS       DATNBV010000418         6938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3061830,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000418 DATNBV010000000
VERSION     DATNBV010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3061830"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VN627_09970"
     CDS             <1..601
                     /locus_tag="VN627_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03621.1"
                     /translation="PVLERYGDALGGKWLGYLSSTGVYGDTGGAWVDETAPVGTGRRT
                     ARSECDREWLARGARVFRLPGIYGPGRSALDRVREGRAHRIAPADSGPGQVFSRVHVA
                     DIASGVMAGLAAAPGAYNLADDLPASQNAVIEEACRLLGQPVPPLLSLEEAGLSPMAR
                     AFYAENRRVANGRARRVLGWRPLYPTYREGLRAVLEAER"
     gene            complement(598..1509)
                     /locus_tag="VN627_09975"
     CDS             complement(598..1509)
                     /locus_tag="VN627_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXD03622.1"
                     /translation="MTVASPPTAKAIPRGVVVPFVLIVAVWSSTWYVIKDQIGLAQPS
                     WTVVARFVVASAGMAVLALLRGESLRLPRAAWPTIGLLGLTQFCLNFQFVYRAELSLT
                     SGIMAIIMALVMIPTALLARVLFGAPVDRRFIAGSAVAVLGIGLLLLHEYRAAPPGAA
                     VLSGLAYGFAAVLSVAVATVLQLSRGVRDCPPIPLMAVVMAVGTLINLGWALAQGGPL
                     PTGLPPRFWGGVVYLAIVCSVVPFPLYVNLIRRIGAGRAAYVNVAIPVFAMVISTVLE
                     AYRWTSLAASGALLAMAGLLIVMRARG"
     gene            1623..1925
                     /locus_tag="VN627_09980"
     CDS             1623..1925
                     /locus_tag="VN627_09980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXD03623.1"
                     /translation="MEGIEIVRLDQGRVGEYHAHIPGSSEVARLTWVLRDTPAGPVRV
                     AEHTLVPRAFEGRGIAARLVDALIADAREAGFRVEPKCSYVAAQFRRHPDWAPLHA"
     gene            complement(1935..2219)
                     /locus_tag="VN627_09985"
     CDS             complement(1935..2219)
                     /locus_tag="VN627_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03624.1"
                     /translation="MYAARWMSLAADSALLAWEGASVIGLRLMRLSMLDARAATEAWL
                     MVEEKLESAALLQWRAMTGDLGRSPHAVASASVRHYRGKVAANRRRLSRR"
     gene            complement(2252..2326)
                     /locus_tag="VN627_09990"
     tRNA            complement(2252..2326)
                     /locus_tag="VN627_09990"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2292..2294),aa:Thr,seq:ggt)
     gene            2452..2604
                     /locus_tag="VN627_09995"
     CDS             2452..2604
                     /locus_tag="VN627_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03625.1"
                     /translation="MANKAEMSREERLAAKLRENLHRRKAQARALDSRQAGESGETLP
                     KPAPSR"
     gene            2745..3431
                     /gene="gpmA"
                     /locus_tag="VN627_10000"
     CDS             2745..3431
                     /gene="gpmA"
                     /locus_tag="VN627_10000"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HXD03626.1"
                     /translation="MPRLILVRHGQSQWNLENRFTGWWDVDLTEKGEAEARAAGTLLR
                     EKGMLPSLAFTSVQTRAIRTLHLALEAAGRVWIPETKDWRLNERHYGGLTGLDKAETA
                     ARHGEDQVKIWRRSFDTPPPVLEAGGPFDLASDPRYAGIAIPSTESLKDTIARVLPYY
                     EAAIAPALKAGETVLVAAHGNSLRALVKHLSGISDADITGLEIPTGQPIVYELDDTLT
                     ALERYYLSER"
     gene            3433..3927
                     /gene="purE"
                     /locus_tag="VN627_10005"
     CDS             3433..3927
                     /gene="purE"
                     /locus_tag="VN627_10005"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HXD03627.1"
                     /translation="MSPSPVQPKVAIVMGSQSDWATMKGAVDVLETLGVVADVRIVSA
                     HRTPERLYHFATTAADHGLKVIIAGAGGAAHLPGMVAALTHLPVLGVPVQSKALSGKD
                     SLLSIVQMPAGIPVGTLAIGEAGAVNAGLLAAAILATNDEGLSGRLQEYRAAQTANVA
                     ERPS"
     gene            3930..4994
                     /locus_tag="VN627_10010"
     CDS             3930..4994
                     /locus_tag="VN627_10010"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HXD03628.1"
                     /translation="MIPPGETIGILGGGQLGRMIAMAAAQLGYRCHVYAPEADSIAAE
                     VCGEFTNAEWDDAAALARFAADCAVITYEFENVPVAPLAALAATPLLPGANALEVAQD
                     RLSEKTFVTDLGGTPAPFAAVDSREDLAAAIARIGTPGILKTRRDGYDGKGQWRVMTG
                     ADAAGLDDAVFARPLVYEGFVTFAAEFSVILCRAANGDVRFWDSAENRHVGGILDRST
                     VPAGAAVAGEVAAARELARAIADRLGYVGVLTCEFFATANGPVFNEMAPRVHNSGHWT
                     IEGAVTSQFENHVRAICGLPLGDTRLAAKGVVMDNLIGDDVHDWPAILSDPANHLHLY
                     GKAAARPGRKMGHVTRLILE"
     gene            4994..5473
                     /locus_tag="VN627_10015"
     CDS             4994..5473
                     /locus_tag="VN627_10015"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HXD03629.1"
                     /translation="MPAPFIIVAAARNGTIGANGTLPWHIPADLKRFKALTMGKSMIM
                     GRKTFDSLPGLLPGRRHVVLTRDPAWQAEGAEVAHDLAEALALAGDEAAVIGGAEIIA
                     LALPVAGRIELTEVHADVPGDTSLPPLGEGWRETFRESHEPSGEKPGFAFVTLARAL"
     gene            5484..6413
                     /locus_tag="VN627_10020"
     CDS             5484..6413
                     /locus_tag="VN627_10020"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HXD03630.1"
                     /translation="MTRLDSRQPLPEQFRGAIIALGNFDGFHLGHQAVVGEAVRWARS
                     EGRPALVATFDPHPKRFFVPDAPSFRLTTLDQREDLFTAAGADAMVVIHFDAAVAAMS
                     GADWVEGILHRQFGAAGVVTGEDFTFGKGRSATADLLGSLGAPLGITARTAPAVFDAG
                     EPVSSTRIRAALQAGDCETATRLLSRPFTIRGTVQHGDKLGRTIGFPTANLPLDHYLR
                     PRYGIYAVKGRLPDGNIYNGAANLGVRPSFDPPKELLEPYFFGWSGDLYGQEIAVELH
                     HFLRPEAKYDGLEALTAAIAADCERAKALLGGA"
     gene            6630..>6938
                     /locus_tag="VN627_10025"
     CDS             6630..>6938
                     /locus_tag="VN627_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXD03631.1"
                     /translation="MTEQRDYRDTVFLPKTDFPMKAGLPQKEPGILSRWQSEGIYQQL
                     RESREGREKFILHDGPPYANGDIHVGHALNHILKDMVVRTQSLMGKDAPYVPGWDCHG
                     L"
BASE COUNT         1116 a         2353 c         2483 g          986 t
ORIGIN      
        1 tcccgtcctc gaacgctatg gtgacgcact ggggggcaag tggctgggct atctctcctc
       61 caccggggtc tatggcgata cgggcggggc ctgggtcgac gagacggcgc cggttggcac
      121 cggacggcga accgcccgca gcgaatgcga ccgggaatgg ctggcgcggg gcgcgcgggt
      181 gtttcgcctg ccgggaatct atggcccggg ccgctccgcg ctggaccggg tgcgcgaggg
      241 gcgggcccac cggatcgcgc ctgccgattc cgggccgggg caggtgttca gccgggttca
      301 cgtggccgac atcgccagcg gggtcatggc cgggctggcg gcggcgccgg gggcctacaa
      361 cctcgccgac gacctgccgg ccagccagaa cgcggtgatc gaggaggcct gccggctgct
      421 cggccagccg gtgccgccgc tgctctcgct ggaggaagcg gggctttcac caatggcgcg
      481 ggcgttttac gccgagaacc ggcgggtggc gaacggcagg gcgcgccggg tgctcggctg
      541 gcggccgctc tatccgacct atcgcgaggg tctgcgcgcc gtgctggaag ccgaacgcta
      601 gccgcgggcc cgcatcacga tcagcaggcc ggccatggcc agcagggcgc cggaagcggc
      661 gagcgaggtc cagcgatagg cctcgagcac ggtcgagata accatggcaa agacggggat
      721 cgcgacgttg acataggccg cccggcccgc gccgatccgg cggatcagat ttacgtagag
      781 cgggaaaggc acgaccgagc agacgatcgc gagatagacc accccgcccc agaaccgggg
      841 cggcaggccg gtcggcaggg gaccgccctg cgccagggcc cagcccaggt tgatcagggt
      901 gccgactgcc atgaccacgg ccatcagggg aatgggcggg caatcgcgca cgccccggct
      961 gagctgcaag accgtggcaa cggcaaccga cagcacggcg gcaaagccat aggcgaggcc
     1021 cgacaggacc gcggcccccg gcggcgcggc gcggtattca tgaagcagga gcaggccgat
     1081 gcccaggacg gccacggccg agccggcgat gaagcggcgg tcgaccggcg cgccgaacag
     1141 cacccgggcg agcagggccg tggggatcat gaccagggcc atgatgatcg ccatgatccc
     1201 ggaggtgagc gacagttcgg cgcggtagac gaactggaaa ttgaggcaga actgggtgag
     1261 gccgagcagg ccgatcgtag gccaggcggc gcggggcagg cgcagcgatt cgccgcgcag
     1321 cagggcgaga acggccatgc ccgcgctcgc cacgacgaag cgggcgacca cggtccagct
     1381 tggctgggca aggccgatct ggtccttgat gacataccag gtggacgacc ataccgcgac
     1441 tatgaggacg aagggcacga cgacgccgcg cggaatggcc ttggcagtgg gcggtgaggc
     1501 gacggtcatg gcggggaaat tcgcatggaa gcatggcagg aacctgcccg ggccgcaccg
     1561 gattgtgcca aggcgcgaga gcggtcaagc tggcgagcgc attacagcga aaggattttg
     1621 cggtggaagg gatcgagatc gtgcggctgg accaggggcg cgtcggggaa taccacgccc
     1681 acattcccgg cagcagcgag gtggcgcggc tgacctgggt gctgcgcgat acgcccgcag
     1741 gccccgtgcg cgtggccgag cacacgctgg tgccgcgcgc cttcgagggc cgcgggatcg
     1801 cggcgaggct ggtggatgcc ctgatcgcgg acgcgcgcga ggcgggtttc cgggttgagc
     1861 ccaagtgctc ctacgttgcc gcgcagttcc ggcgccatcc cgactgggcg ccgcttcacg
     1921 cctgaaggcg tggctcagcg ccggctcagc cgcctgcggt tggcggcgac cttgccgcga
     1981 tagtgcctga cgctggcgct ggcgacggca tggggactgc gcccgagatc gccggtcatc
     2041 gcccgccact ggagcagggc cgcgctctcc agcttttcct cgaccatcag ccaggcctcg
     2101 gttgcagcgc gggcatcgag catggacagg cgcatcagcc tgaggccgat caccgaggcc
     2161 ccttcccagg ccagcagggc gctatcggcg gcaagggaca tccagcgcgc tgcgtacatc
     2221 gttacgatcc gattctggag aatgggggaa atggtgctgc tggggaggat tgaactcccg
     2281 acctcagcct taccaaggat gcgctctacc actgagctac agcagcacac catttccgcc
     2341 gcgccggggc catgcggctc cgggcgaggc ggcgctattg gccgcgaggg ggcggcttgt
     2401 caaggcgaac cctgcttgca tgagcgcggc gcccacggca gggaggcgag catggcgaac
     2461 aaggcggaaa tgagccggga agaacggctg gcggcaaagc tccgggagaa cctgcaccgg
     2521 cgcaaggccc aggcgcgggc gctcgattcc cgccaggcgg gcgaatcggg cgaaaccctt
     2581 cccaaaccgg cccccagccg ctaggggcgc tgcaagtttg tctgtccgcc tgacggcgga
     2641 ggcggcaccg gcccgctccc ccgcccggcc cacccggcca agcatattgc catgggtggc
     2701 gttacggggg agcgggccgg tgccgcaaac cttcggagca aagaatgccg cgtctgattc
     2761 tcgtccgcca cgggcaaagc cagtggaacc tcgagaaccg tttcaccggc tggtgggatg
     2821 tggacctgac cgagaagggc gaggccgagg cccgcgccgc cgggaccctg ctgcgcgaga
     2881 agggcatgtt gcccagcctg gcgttcacgt ccgtccagac ccgcgcgatc cgcacgctgc
     2941 acctggcgct ggaagcggcc gggcgggtgt ggatcccgga aaccaaggac tggcgcctga
     3001 acgagcggca ctatggcggg ctgaccgggc tcgacaaggc cgaaacggcg gctaggcatg
     3061 gcgaggacca ggtgaagatc tggcgccgtt cgttcgacac gccgccgccg gtcctggagg
     3121 cgggcgggcc gttcgacctg gcctctgatc cgcgctacgc cgggatcgcc atcccttcga
     3181 ccgagagcct caaggacacc atcgcgcgcg tcctgcctta ttatgaggcg gcgatcgccc
     3241 ccgcgctgaa ggcgggcgag accgtgctcg tcgccgccca cggcaattcg ctgcgggcgc
     3301 tggtcaagca cctttccggc atttcggatg ccgacatcac tggcctcgag atccccaccg
     3361 gccagccgat cgtctacgag ctggacgaca ccctcaccgc gcttgagcgg tactacttgt
     3421 cggagcgttg agatgagccc ctcccccgtg cagcccaagg tcgccatcgt catgggcagc
     3481 cagtccgact gggccacgat gaagggcgcg gtcgacgtgc tcgagacgct cggcgtcgtc
     3541 gccgacgtgc ggatcgtctc ggcgcaccgt acgcccgagc ggctctatca ttttgcgacg
     3601 accgcggccg accacggcct caaggtgatc atcgccggcg cgggcggcgc ggcgcacctg
     3661 cctggcatgg tcgcggcact gacccacttg ccggtgctgg gcgtgccggt ccagtcgaag
     3721 gcgctctcgg gcaaggacag cctgctctcg atcgtgcaga tgcccgccgg aatcccggtc
     3781 ggcaccctgg cgatcggcga ggccggggcg gtgaacgccg ggctgctcgc cgccgcgatc
     3841 cttgccacca atgacgaggg cctctccggc cgcctccagg aataccgcgc ggcgcagacc
     3901 gccaacgtgg ccgaacggcc cagctgagca tgatcccccc cggcgagacc atcggcatcc
     3961 tcggcggcgg ccagcttggg cggatgatcg ccatggcggc ggcgcagctg ggctaccggt
     4021 gccacgtcta tgcgccggaa gcggactcca tcgccgcgga ggtctgcggg gagttcacca
     4081 atgccgagtg ggacgatgcg gcagccctcg cccgcttcgc cgccgactgc gcggtgatca
     4141 cctacgagtt cgagaacgtg ccggtcgccc cgctggccgc gctggccgcg acaccgctgc
     4201 tgcccggggc caacgcgctc gaagtggcgc aggaccggct gagcgagaag actttcgtca
     4261 ccgatcttgg cgggaccccc gccccgttcg cggcagtcga ttcgcgcgag gacctcgccg
     4321 cggccatcgc ccggatcggc acgcccggca tcctcaagac ccggcgcgac ggctatgacg
     4381 gcaagggcca gtggcgggtc atgaccgggg ccgatgccgc cgggctggac gacgcggtgt
     4441 tcgcccgccc gctggtctac gagggcttcg tcaccttcgc cgcggagttc tcggtgatcc
     4501 tgtgccgcgc cgccaatggc gacgtgcgct tctgggattc ggccgagaac cggcacgtgg
     4561 gcggcatcct cgatcgctcg accgtgcccg ccggcgcggc ggtggccggc gaggtggcgg
     4621 cggcgcggga gctggccagg gcgattgccg accggctcgg ctatgttggc gtgctgacct
     4681 gcgagttctt cgcgacggcc aatggcccgg tgttcaacga gatggcgccg cgcgtccaca
     4741 actccgggca ctggacgatc gagggcgcgg tcaccagcca gttcgagaac cacgtgcgcg
     4801 cgatctgcgg gctgccgctg ggggataccc ggctggcggc aaagggcgtg gtgatggaca
     4861 acctgatcgg cgacgatgtt cacgactggc cggcgatcct gtccgacccg gccaaccacc
     4921 tgcaccttta cggcaaggcg gcggcccggc ccgggcgcaa gatgggccat gtgacgcggc
     4981 tgatcctcga gtgatgcctg ctcccttcat tatcgtggcc gccgcgcgca atggcaccat
     5041 cggcgcgaac ggcaccctgc cctggcacat tccggcggac ctcaagcggt tcaaggcgct
     5101 gaccatgggc aagtccatga tcatgggccg caagaccttc gattcgctgc ccggcctctt
     5161 gcccgggcgg cggcacgtgg tgctgacccg cgacccggca tggcaggccg aaggcgccga
     5221 agtggcgcac gatctggctg aggcactggc gctcgcaggc gatgaagcgg cggtgatcgg
     5281 cggggccgag atcatcgccc tggcgctccc cgtggccggc cggatcgaat tgaccgaagt
     5341 gcacgccgac gtcccgggcg acacgtccct ccccccgctg ggcgagggct ggcgcgagac
     5401 attccgggaa agtcatgaac cttcgggcga aaaaccgggc ttcgctttcg tgacgctggc
     5461 aagggctcta taggcgcgcc cgcatgactc gcctggacag ccgccaaccc ctgcccgaac
     5521 agttccgcgg cgcgatcatc gcgctgggca atttcgacgg gtttcacctc ggccaccagg
     5581 ccgtggtcgg cgaggcggtg cgctgggccc gcagcgaagg ccgcccggcg ctggtcgcca
     5641 ccttcgatcc ccatcccaag cggttcttcg tgcccgacgc gccgtcgttc cgcctgacca
     5701 cgctcgacca gcgcgaggac ctgttcaccg ccgccggggc cgatgccatg gtggtgatcc
     5761 acttcgatgc cgccgtcgcc gcgatgagcg gggcggactg ggtggagggc atccttcacc
     5821 gccagttcgg cgcggcgggc gtcgtcaccg gcgaggattt cactttcggc aagggccgca
     5881 gcgcgacggc cgacctgctc ggctcgctgg gcgcgccgct gggcatcacc gcgcggaccg
     5941 caccggcggt gttcgacgcg ggcgaaccgg tctcctccac ccgcatccgc gcggccttgc
     6001 aggcgggcga ctgcgagacg gcgacccgcc tgctctcgcg cccgttcacg atccgcggca
     6061 cggtccagca tggcgacaag ctggggcgca cgatcggctt tcccacggcc aacctgccgc
     6121 tcgatcacta tctgcggccg cgctacggga tctatgcggt gaagggccgg ctgcccgacg
     6181 gcaatatcta caacggcgcg gccaacctgg gcgtgcggcc gagcttcgat ccgcccaagg
     6241 agctgctgga gccttatttc ttcggctggt cgggcgatct ctatggccag gaaatcgcgg
     6301 tggagctgca ccacttcctg cggcccgagg ctaagtatga tgggctggag gcgctgacgg
     6361 cggcgatcgc ggcggattgc gagcgggcga aggcgctgct gggcggggct tagcgctttt
     6421 cagggttcac gcagagttcg cagaaaagaa ggggagacgc agaggaggca gcgccatgcg
     6481 gcgcagccgc tctttcaatg cttcccgtgc ggttccagcg aacaggccgc tgcgcggcac
     6541 aacctctctg cgcctctctg cttcccctcc ggcctctgcg tgaaccaaaa tttgcgctcc
     6601 ctcgcaggca aagctaaggc ccgccgatca tgaccgagca gcgcgactac cgagacaccg
     6661 tcttcctccc gaagaccgat ttccccatga aggccgggct tccccagaag gagccgggca
     6721 tcctgtcgcg ctggcagtcc gagggcattt accagcagct gcgtgaaagc cgcgagggcc
     6781 gggaaaagtt catccttcat gacggcccgc cctatgccaa tggcgacatc cacgttggcc
     6841 atgcgctcaa ccatatcctc aaggacatgg tggtgcgcac ccagagcctg atgggcaagg
     6901 acgcgcccta cgtgcccggc tgggactgcc acgggctc
//
LOCUS       DATNBV010000419         3382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_872930,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000419 DATNBV010000000
VERSION     DATNBV010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872930"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="VN627_10030"
     CDS             complement(<1..1416)
                     /locus_tag="VN627_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HXD03632.1"
                     /translation="MLAAVALAAGVLALGPIGCAPGDDNGASHGAGTGLGISLDALDK
                     TVKPGDDFYAYANGAWMRSATIPADKSSTGAFREADDATQKQLGKLVDDIIKGDAAPD
                     SDPGRIRTYYTTYVDTRAIDAAGLTPARADLDRFAAIADKAALSRVLGEQLRADVDPL
                     NATDWQTENLFGLFVTQSLSGSAVMPYVLQGGLGMPGRDYYLSPDPKMAAIRDKYKAY
                     LAQFLTAAGFADAPAMAARIYALEAKIAAAHATLVQSNDFRSGAGEWTQAEFAKKAPG
                     IDWPAFFAGAGLAGQDRFVAYHATAIPKLAALVASEPLQAWKDWLAFHQLNSHADVLP
                     GKLDAAHFGFYEGVLNGTAEPTPRAKRGIEALNAALGDALGKLYVEEYFPASAKAEIE
                     GMVGAIKGAFAARIEALDWMADATRREALTKVNAIAVGIGYPDKWTDYTALTILPGQA
                     YANEVAAEKFRLAQQLAKIGKP"
     gene            1729..1917
                     /locus_tag="VN627_10035"
     CDS             1729..1917
                     /locus_tag="VN627_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03633.1"
                     /translation="MSEALLILPDNGPNPSGLWRQLDQCLAGLDAIGADIAAAHLAMA
                     MDRLRIQFNLDEDGSGTD"
     gene            2072..2677
                     /locus_tag="VN627_10040"
     CDS             2072..2677
                     /locus_tag="VN627_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXD03634.1"
                     /translation="MPDHTGNGADWPAAPMCSAIPLRPAGDVQPIVVPAGRDQARRLI
                     AAANELLALAAELEQGAAPAPAGDGYVPSTEFAPEDDGRWLRLARHTYRRRRSRILFF
                     EESLFGEPAWDLLLDLFIAAKERKRVPVTSACIGAAVPTTTALRWLALLEDRGLVVRE
                     ADPTDARRIFVRLTAEAYAKMVAYFARAAAGESDETVGAGR"
     gene            3133..>3382
                     /locus_tag="VN627_10045"
     CDS             3133..>3382
                     /locus_tag="VN627_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HXD03635.1"
                     /translation="MPIDFCLIITTANFGGSPAPRDLSRRLIFVTAHAWGDEPPELDW
                     SAVADPAATLAPWPERCPRHLKQRIAAGLSPGTALALIH"
BASE COUNT          510 a         1171 c         1129 g          572 t
ORIGIN      
        1 cggcttgccg atcttggcga gctgctgggc gaggcggaat ttctcggcgg cgacttcatt
       61 ggcataggcc tggccgggga ggatcgtcag cgcggtatag tcggtccact tgtcgggata
      121 gccgatgccg accgcgatgg cgttcacctt ggtcagcgcc tcgcgccggg tcgcatcggc
      181 catccaatcc agcgcttcga tccgcgccgc gaaggcgccc ttgatcgcgc cgaccatccc
      241 ctcgatctcg gccttggccg aggcggggaa atactcctcc acgtagagct tgccgagcgc
      301 atcgccgagt gcggcgttga gcgcctcgat cccgcgtttg gcccggggcg tgggctcggc
      361 cgtgccgttg aggacgcctt cgtagaaccc gaagtgggcg gcatcgagct tgcccggcag
      421 gacatcggcg tggctgttga gctggtggaa ggccagccag tccttccagg cctggagcgg
      481 ttcgctggcc accagcgcgg cgagcttggg gattgcggtg gcatgatagg cgacgaaccg
      541 gtcctgcccg gcgaggcccg ccccggcgaa aaaggcgggc cagtcgatcc ccggtgcctt
      601 cttggcgaat tccgcctggg tccactcgcc ggcgcccgaa cggaaatcgt tgctctggac
      661 cagcgtggcg tgggcggcgg cgatcttcgc ttcgagcgca tagatccgcg cggccatggc
      721 gggcgcatcg gcgaagccgg cggcggtgag gaactgggcc agataggcct tatacttgtc
      781 gcggatcgcc gccatcttgg gatcgggcga gaggtaatag tcccgccccg gcatgccgag
      841 gccgccctgc agcacatagg gcatgacggc gctgccgctg agcgactggg tcacgaacag
      901 gccgaacagg ttctcggtct gccagtcggt cgcattcagc ggatcgacgt cggcacgcag
      961 ctgttcgccg agtacgcgcg acagggccgc cttgtcggct atggcggcga agcggtcgag
     1021 atcggccctg gccggggtca gcccggcggc gtcgatcgcc ctggtgtcga cataggtggt
     1081 gtaataggtg cggatccggc cgggatcgct atcgggagcg gcatcgccct tgatgatatc
     1141 gtcaaccagc ttgcccagct gcttctgggt ggcatcgtca gcctcgcgga aggccccggt
     1201 gctcgacttg tcggccggga tggtggcgct gcgcatccat gcgccgttgg cataggcgta
     1261 gaaatcgtca cccggcttga ccgtcttgtc gagcgcgtcg agcgagatgc cgagacccgt
     1321 gccggcgccg tgcgacgccc cgttatcgtc accaggcgcg cagccgattg gtccgagcgc
     1381 caggaccccc gcggccaggg ccacggcggc gagcacggca tgaacaggca ggcgaagcgg
     1441 gcgcaggagc aaacggacct cagctggatg gcgaccagac cggccccgcg cccgcgcacc
     1501 ggtgaccggc ggcggacttg cgggacttcc ctatagcgca gcctcccgcc cccgccaccc
     1561 cgcattcgca cattccgccg aacgggcgat tcatcctgcc gatgacctga cagttgtggt
     1621 atccgggttt gcgcggacaa ctgccgcaag actagttgca ccggttgcgt ccatattggt
     1681 gcaatgccgt cccgccagag aaggtcggcc cggttccttg gagctgccat gtccgaagcc
     1741 ctgctgatcc tgccggataa cggacccaac ccctccgggc tgtggcggca gctcgatcaa
     1801 tgcctcgccg gactcgacgc cataggtgcc gacattgccg ccgcccacct tgccatggcg
     1861 atggatcggc tgcgcattca gttcaatctg gatgaggatg gctcgggaac ggactagtct
     1921 ccgctccccg caatcggttg ccgagctaga gttcccacca gcagcaggtc ccgggggtgt
     1981 cagggtctgc tgccgatctg gggctcgttc gataatggtc gggtatagtg gggaattgga
     2041 acatctgcgg ggcctcggcc cggggcaggc catgcccgat catacgggca acggcgccga
     2101 ttggccggcg gcgcccatgt gttcggccat tcccttgcgt ccggcagggg atgtccagcc
     2161 catcgtcgta cccgccggtc gcgaccaggc gcgccgcctg atcgccgcag ccaatgaact
     2221 gctggcgctc gccgccgaac tggaacaagg cgctgcgccc gcccctgctg gcgacggcta
     2281 cgtcccgtcg accgaattcg ccccggagga tgatggccgc tggctgcgcc ttgcccggca
     2341 tacctaccgc cgccgccgca gccgcatcct gttcttcgag gaatcgctgt tcggcgaacc
     2401 ggcctgggat ctgctgctcg acctgttcat cgccgcgaag gagcgcaagc gggttcccgt
     2461 caccagcgcc tgcatcggcg cagccgtccc cacgacgacg gccctgcgct ggcttgcctt
     2521 gctggaagac cgcggcctcg tggtccgcga agccgatccg accgacgccc gccggatctt
     2581 cgtgcgcctc accgccgaag cctatgccaa gatggtcgcc tacttcgccc gcgccgccgc
     2641 tggcgagagc gatgagactg tcggcgcggg gcgatagcat cctccgcgct gttccaaaca
     2701 attcgcgcga cgccggcagc tcgatccgcg tcctgggagg attaccggag gcgcggcagc
     2761 ctttcccccc acgccagcgc gccgtgcggc cggcgcgaac cactgcgctg gaaagcgttt
     2821 tatcggagcg atgcattgtt ccccgggcga cggatgccac gcgccaccgt gctatccccc
     2881 gcctgggaaa atcaccggtt cgggtgtgcc ttgcccgcac tgttcaggcc cgggttgcgc
     2941 cggcgcgaac cgctgcgcct gaatgtgttg tatcggggca atgggtagat ccccgccggc
     3001 cgatacctac cgcccgcgcg tcatccgcca cctaaggaaa ctgcctggct cgggcgtccc
     3061 ttgccgcacg gttcaggccg cccgcgatct ccggtcggcg gacgaggaat tactgcatac
     3121 cgcgcggccg gtgtgccgat cgatttctgc ctcatcatca ccaccgccaa cttcggcggt
     3181 tcgcccgccc cgcgcgacct ctcacgcagg ctgattttcg tcaccgctca cgcatgggga
     3241 gacgagccgc cggagctgga ctggagcgca gtggcggacc ctgccgccac gctcgcccca
     3301 tggccagagc gctgcccacg ccatctcaag caacgcatcg ccgccggatt gagccccggt
     3361 accgccctcg ctctcatcca cg
//
LOCUS       DATNBV010000420         2472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4712808,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000420 DATNBV010000000
VERSION     DATNBV010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4712808"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..349
                     /locus_tag="VN627_10050"
     CDS             182..349
                     /locus_tag="VN627_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03636.1"
                     /translation="MNAVGQVARWLERADPPRSGRATPYGAVVRAIAWHLRLGGPWRA
                     LPPGGPHWRTV"
     gene            657..2303
                     /locus_tag="VN627_10055"
     CDS             657..2303
                     /locus_tag="VN627_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HXD03637.1"
                     /translation="MHVAIYARVSTQRQAQAQTTDQQLDRLRAHAEGQGWSVTGDRVF
                     RDDGYSGASLKRPGLDRLRDAATTARLDRILITAPDRLARNYVHQVLLVEELQKHGAE
                     VVFLDRPMSRDPHDQLLLQIRGAVAEYERTLIAERMRRGRLRKLRAGTLLPWTRPPYG
                     YRLDPERPRDPAGVRLDEAEAAVVRDLFAWFADEGAAVFTLVQRLHRLGIASPRGHQT
                     WSPSSLRGVLSNPLYLGQVFANRLRTHPAERRRSALLPLGRGQGAKRVADPAEWIAVA
                     PVPAIIGQEQFDRAQERLAYNRQMARRNNRVHPYLLRGLVSCGRCRRGCIGRHMRTDL
                     DYYLCRTKTQARLLTPGERCPARYIPARALEDLVWRDLCEVLSAPHMIAHAMERARGG
                     HWLPQELQARQANLRRGRAALNHQVERLTEAYLAGVVPLCEFERRRRDVETRLQALDR
                     QEHDLVHEAERHGETARLAAHAEAFCRRVREGLAEAGFDQKRALLELLIDRVIVTDGA
                     VEIRYVVPTSPEGECQPFWRLRTDYLCCCSGGGQNPCAFV"
BASE COUNT          397 a          808 c          847 g          420 t
ORIGIN      
        1 tcacaatcgg gatgatttta ctctggatct agagcgtttc cgacttagag cctgtttgag
       61 tgccctgtat ggctgatccc tatctgatcc cgcagtgacc atggtggttt gactgagggg
      121 acagatgtgg accgaggcgc atcgggcgcg ccatgacgcg cgcctgaaag agatagtgtc
      181 gttgaacgca gtagggcagg tggcgcgatg gctggagcga gcagacccgc cgcgcagcgg
      241 gcgggcaacg ccctatgggg cggtcgtgcg cgcgatcgct tggcacctgc gcctcggcgg
      301 cccgtggcgg gcgctgccgc caggcgggcc gcactggcgc accgtgtaga tggtctgttc
      361 acaaccgaca aattctgcct gacgcggcag ggcacgttgg cgctgtcgag gcagcgcagt
      421 ccactggggg ctgatcgccc aacacccaac gctggtgctg ctgttccgag agggtcttgc
      481 cctctcggag tggaccatga agcaacagca ctggacggtg gcgcggggcc aagtcagcca
      541 cccggcagcc gcgcggcggt gggaccaggc ctaccagcag ctcctgtcga tgcacgccgc
      601 gggtccgggc gtccccgaaa ccgcgactcc gacgcgccta tcggaggaga gtcgccatgc
      661 acgtagccat ctacgccagg gtctcgaccc agcgccaagc ccaggcccaa accacggacc
      721 agcaactcga tcgcctccgc gcccatgccg aggggcaggg ctggagcgtg accggggatc
      781 gtgtcttccg ggacgacggc tatagcgggg ccagcctcaa acgccccggc cttgatcgcc
      841 tgcgcgatgc cgctacgact gcccggctgg atcggatcct gatcacggct ccagatcggc
      901 tggcgcgcaa ctacgtgcac caggtgcttc tggtcgagga gttgcaaaag cacggggcgg
      961 aggtggtgtt cttggaccgg cccatgagcc gcgacccgca cgatcaactc ctgctgcaga
     1021 tccgcggcgc cgtggccgag tatgagcgca ccctgattgc cgagcgcatg cgccggggtc
     1081 gcctgcgcaa gctgcgcgcc ggaaccctct tgccctggac gcgaccgccc tatggctacc
     1141 ggctcgatcc cgagcggccc cgcgacccgg ctggggtacg actggacgag gccgaagctg
     1201 cggttgtccg ggacctgttc gcttggttcg ccgatgaagg agcggcggtc ttcaccttgg
     1261 tccagcggct gcaccggctg ggaattgcgt cgccccgggg ccaccagacc tggagcccct
     1321 cgtccttgcg aggcgtgctc agcaaccctc tctacctcgg gcaggtgttt gctaatcgcc
     1381 tccgcacgca tcccgccgag cggcgccgct ctgccctcct gccgctggga cgcggtcaag
     1441 gggccaagcg ggttgcggac ccggccgagt ggatcgcggt cgcaccggtg cccgcgatca
     1501 tcggccaaga gcagttcgat cgcgcccagg agcggctggc ctataatcgc cagatggcac
     1561 gccgaaacaa ccgggtgcat ccgtatcttc tgcgcggcct ggtcagctgc ggccggtgcc
     1621 gtcgtgggtg catcggccgc cacatgcgga cggacctcga ttattatctc tgccggacca
     1681 agacgcaggc gcggctgctg acccccggcg agcgctgccc agcccgctac atcccggccc
     1741 gtgctctaga ggatctggtc tggcgcgacc tgtgcgaggt cctgtcggcc ccgcacatga
     1801 tcgcgcacgc gatggaacgg gcgcgcggtg ggcactggct tcctcaagag ttgcaggccc
     1861 gccaagccaa cctgcgccgg ggccgggcgg ccctcaacca ccaggtcgaa cggctcacag
     1921 aggcgtatct cgccggggtc gtgcctctgt gcgagtttga gcggcgtcgg cgcgatgtcg
     1981 agacccggct gcaggcgctg gaccgccagg agcatgacct cgtgcacgag gcagagcggc
     2041 acggcgagac agcccggctg gccgctcatg ccgaggcgtt ctgccgccgt gttcgggagg
     2101 gcttggcgga agccggcttc gatcagaagc gggcgctgct cgaactgctg attgaccgcg
     2161 tgatcgtcac cgacggtgcc gtcgaaatcc gctacgtcgt cccaaccagc ccggaaggtg
     2221 aatgccaacc cttttggcga ttgcgaacag actatttatg ctgttgttcg ggaggggggc
     2281 aaaacccgtg tgctttcgtt tgagccccat ctcggacgtg aagccgcaac gcttcaaagc
     2341 tttgaagatt tccgcaactt ccacctgagt gagaccattc aggtgatcgg cccccatggg
     2401 gtggtccggt ttgaatgtta gtgcatggtt ggtttgggcg ctacggggag tctggccggc
     2461 ggagccggtc gg
//
LOCUS       DATNBV010000421         2674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_547476,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000421 DATNBV010000000
VERSION     DATNBV010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547476"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="VN627_10060"
     CDS             complement(<1..1526)
                     /locus_tag="VN627_10060"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXD03638.1"
                     /translation="MFRAHDQELPLGGVAPAAPTGLPGLPGPGRVPSLRSFRALRLDT
                     SDWVVDLAEGIGTRRWWRGMATMAGFALAAFTFWPGMSRLEAAPATHAAAAQPGEWRS
                     LGAVPLSYDTTRLLQRAPATGALRAVAFVPERPQLAFTSVLAGGDDLASLLRRAGAAD
                     GDARQAGALVAGAVSADQLAPATRVALVLGPRPAPGQPRPLQRVELRARLDLALAVNR
                     VGGGLALERRPIPVDTTPLRIRGMVGASLYRSARAAGAPPQAIQEYLQALDANLNLDS
                     DVLPTDSFDLVFAYKRAATGESEPGELLYAGLDRGKQPVAQLLRWGHDGAFYSADALA
                     QPRYVTSGYSGAGLLTPVNGRITSLFGQRFHPILGYSRMHAGVDFGAAWGSPIVASAA
                     GVVSYAGYHGGHGNYVRLEHGGGLGTGYGHMSRIAVTPGERVSAGEVIGYVGSTGLST
                     GPHLHYEMYRDGRTVNPLGQSFASVTVTRQVDAGQLAAFRTRLAQLRALRPGPALAAM
                     G"
     gene            1666..>2674
                     /locus_tag="VN627_10065"
     CDS             1666..>2674
                     /locus_tag="VN627_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXD03639.1"
                     /translation="MPHLVVPMAYSLPGSATRAHASGHSGSDGRVKAVLGPTNTGKTH
                     LAIERLCAHSSGAIGFPLRLLAREVYERVVAIKGRDRVALITGEERIEPPNARWSLCT
                     VEAMPRRPDLAFVALDEAQLGADRERGHVFTDRLLHTRGREETMILGSGVLEPMVKAL
                     VPEAEVVSRPRFSTLSHAGTKKLSRVPPRSAIVAFSAEQVYAVAEMLRRFRGGAAVVM
                     GALSPETRNKQVALFQSGEVDYLVATDAIGMGLNLDVTHVAFAGLSKFDGVRNRRLTT
                     AEMAQIAGRAGRHQRDGTFGTLAGAGGHDAEFTDEEVWAIEEHRFPPLTRLFWRDADP
                     RV"
BASE COUNT          426 a          895 c          995 g          358 t
ORIGIN      
        1 cccatggcgg ccagcgccgg cccggggcgg agcgcgcgca gctgcgccag cctcgtgcgg
       61 aaagcagcga gttggccggc atcgacctgg cgggtgacag tcacgctcgc gaagctctgg
      121 ccgagcgggt ttaccgtgcg cccatcgcgg tacatctcgt aatgcaggtg cgggccggtc
      181 gagaggccgg tcgatccgac gtagccgatg acctcgcccg cgctgacccg ctcgcccggg
      241 gtaacggcga tgcggctcat gtgcccatag ccggtgccga ggccgccgcc gtgctcgaga
      301 cggacgtaat tgccgtggcc gccatgatag ccggcatagg acacgacccc tgccgccgag
      361 gcgacgatcg gcgatcccca ggccgcgccg aaatcgacgc cggcatgcat gcgcgaatag
      421 ccgaggatcg ggtgaaaacg ctggccaaag agcgaggtga tgcggccgtt caccggagta
      481 agcaggcccg ccccgctgta gcccgaagtg acatagcgcg gctgggccag ggcatcggcg
      541 gaatagaagg ccccatcgtg gccccagcgc agcagctggg cgacgggctg cttgccgcga
      601 tcgagcccgg catagagcag ttcgcccggc tcgctctcac ctgtggcggc gcgcttgtag
      661 gcgaagacga gatcgaacga atcggtgggc aggacatcgc tgtcgaggtt caggttggca
      721 tcgagcgcct ggaggtattc ctggatcgcc tgcggcgggg caccggcggc gcgggccgag
      781 cggtagagac tggcaccgac catgccgcgg atccgcagcg gcgtcgtatc gacggggatc
      841 ggccggcgct cgagcgcgag accgccaccg acgcgattga cggcgagggc aaggtccagc
      901 cgggcgcgca gctccacgcg ctgcagcgga cgcggttggc ccggtgccgg gcgcgggccg
      961 aggacgaggg caacgcgggt ggcgggcgcg agctggtccg cggaaacggc ccccgcgacg
     1021 agcgcgccgg cctgccgggc atcgccatcc gcggcgccgg cgcggcgcag gaggctggcg
     1081 agatcgtcgc cgccggcgag aaccgaggta aaggcgagtt ggggccgctc cggcacgaaa
     1141 gccacggcac gcagggcccc cgttgccggg gcgcgctgga gcaggcgcgt cgtatcgtag
     1201 gacagcggca cggcgccgag gctgcgccat tcgcctggct gcgcagcggc ggcatgggtg
     1261 gcgggagcag cttcgaggcg ggacatgccc ggccagaaag taaaggcggc aagagcaaac
     1321 ccggccatgg tcgccatgcc ccgccaccag cggcgcgtgc cgataccttc ggcgaggtcg
     1381 accacccagt cggacgtatc caggcgcaga gcgcggaacg agcgcaagga ggggacgcgg
     1441 cccggtcccg gcagaccagg caggccggtc ggagccgccg gagcgacacc gccaagcggc
     1501 agttcctgat cgtgcgctct gaacacgggc cctccgcggg aattcgcgta ggagccggtg
     1561 tgcaccctca gggttaacac gagcctaagt tgcgctgaac ggagcccgca agctgtcgaa
     1621 aaccgaaaag ggggcagtgc ctgcagttgc ggggaggctg ggccgatgcc acatctggtg
     1681 gtgccgatgg cctattctct gcctggatcc gccacccgcg cgcatgcctc tggccattcg
     1741 ggcagcgatg gccgcgtgaa ggcggtgctt ggccccacca ataccggcaa gacccacctc
     1801 gccatcgaac ggctctgcgc ccactcctct ggcgccatcg gatttccgct gcggctgctg
     1861 gcgcgcgaag tctacgagcg ggtcgtggcg atcaagggtc gcgaccgcgt ggccctgatc
     1921 accggcgagg agcggatcga accgcccaat gcgcgctggt cgctgtgcac ggtggaggcc
     1981 atgccgcgcc ggccggatct cgccttcgtc gcgctcgacg aggcgcagct tggcgcggat
     2041 cgcgagcgcg ggcacgtgtt caccgatcgg ctgcttcata cccgggggcg cgaggagacg
     2101 atgatcctgg gctcgggcgt gctcgagccg atggtcaagg cactggtgcc cgaggccgag
     2161 gtggtcagcc ggccgcgttt ttccacgctc tcccatgcgg gcacgaagaa gctctcccgc
     2221 gtgccgccgc gcagcgccat tgtcgccttc tcggccgagc aggtctatgc cgtggccgag
     2281 atgctgcggc gtttccgcgg cggggcggcc gtggtcatgg gggcgctttc acccgagacg
     2341 cgcaacaagc aggtggcgct gttccaatcg ggcgaggtcg actatctcgt cgccaccgac
     2401 gcgatcggca tgggcctgaa cctcgacgtc acccacgtgg cctttgccgg gctttcgaaa
     2461 ttcgacgggg tgcgcaaccg gcggctgacg accgccgaga tggcccagat cgccggtcgg
     2521 gccgggcgcc accagcgcga tggcaccttc ggcaccctgg ccggcgccgg cggacacgac
     2581 gcggaattca ccgacgagga agtctgggcg atcgaggaac accggttccc gccgctcacc
     2641 cggctgttct ggcgcgatgc cgatccgcgg gtgg
//
LOCUS       DATNBV010000422         2672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1524032,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000422 DATNBV010000000
VERSION     DATNBV010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524032"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VN627_10070"
     CDS             complement(<1..1090)
                     /locus_tag="VN627_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXD03640.1"
                     /translation="MTDTVIQPRPAGHPLAGERMSGARMIMQVLADEGVTAIFGYSGG
                     AILPTYDAVFLYNEAAGRLGEPQIPLIVPANEQGAGFMAAGYARASGRVGVCLVTSGP
                     GATNTVTPVRDCMADSIPIVVICGQVSRAAIGTDAFQEAPVPSIMGAVAKHIFLVTDP
                     AKLEATVRTAFEIARTGRPGPVVIDIPKDVQNWEGPFRGEGLLPIPGYRRRMRELTEA
                     ALETAAASRFFSMLGEARRPLIYAGGGVINGNAAPALTAFARAFGIPVVTTLMGIGSV
                     DTTQPLALRMLGMHGAAFANYAVDDCDFLIAVGSRFDDRVAGVPEKFARNALRIAHLD
                     VDRAEVGKVKRVQWSHVGLVAPALEALTA"
     gene            1241..1756
                     /gene="ilvN"
                     /locus_tag="VN627_10075"
     CDS             1241..1756
                     /gene="ilvN"
                     /locus_tag="VN627_10075"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004697653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HXD03641.1"
                     /translation="MRHIIAILLQNEAGALTRVAGLFSTRGYNIESLSVAPTDDPTVS
                     RLTLVTRGSDSVIQQIANQLNKLVDVVNVLDMTRGEHLERELVLLKLKVEAGQADAVR
                     GQVVRGGGRVLDPAPDGFIVELTASEAEINGFVADLGSRAELVEVVRSGALGISRNAR
                     PLRARPAPALL"
     gene            complement(1765..>2672)
                     /locus_tag="VN627_10080"
     CDS             complement(1765..>2672)
                     /locus_tag="VN627_10080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HXD03642.1"
                     /translation="LALVAAGALAAGASYLLNNAAYAHFAGGFAAYSRYVSPWIEEAL
                     KAALLVFLIRTRRVGLPVDAAIAGFAIGTGFALVENIYYLMSQSHTALPVAVIRGFGT
                     AIMHGGTAAVLAMISNTLYERRPSGGLYLLLPGFLAAVALHTGFNCLLVRPALATLAT
                     LLVLPFVIYLVFDHSERILRNWIEVDLESDVKLLESISAGTFLGSNSGRYLQALRDRF
                     RGEELADMLCYLRVHGELAVRAKGVLLMREAGLPELPIEAETRDRLTEFAHLERAIGK
                     AGVLALRPLMMATGKEVWQLTLLRP"
BASE COUNT          461 a          930 c          888 g          393 t
ORIGIN      
        1 gcgcggtcag cgcctccagc gccggcgcca cgagcccgac gtggctccac tgcacgcgct
       61 tcaccttgcc cacttccgcg cggtcgacgt ccaggtgcgc gatgcgcagc gcgttgcgcg
      121 cgaacttctc gggaacaccg gcgacgcggt catcgaagcg cgagcccacc gcgatcagga
      181 agtcgcagtc atcgaccgca tagttggcga acgccgcccc gtgcatgccc agcatgcgca
      241 gcgccagcgg ctgcgtggtg tccaccgagc cgatgcccat gagcgtcgtg accacgggaa
      301 tgccgaaggc gcgcgcgaac gcggtgagcg cgggcgcggc gttgccgttg atgaccccgc
      361 cgccggcgta gatgaggggc cgccgcgcct cccccagcat gctgaagaag cggctggcgg
      421 cggcggtctc gagcgcggct tcggtgagct cgcgcatcct gcgccggtag ccgggaatcg
      481 gcagcagtcc ctcgccccgg aaggggcctt cccagttctg cacgtccttc gggatatcaa
      541 tcaccaccgg cccggggcgc ccggtgcggg cgatctcgaa ggcagtgcgc acagtggcct
      601 cgagcttcgc cggatcggtg acgagaaaga tgtgcttggc gacggcgccc atgatcgagg
      661 gcaccggggc ctcctggaag gcatcggtcc cgatcgcggc acgcgacacc tggccgcaaa
      721 tcacgacaat gggtatcgaa tccgccatgc agtcgcgcac gggtgtaaca gtgttggtcg
      781 cccccggccc ggaggtcacc aggcacaccc ccaccctgcc gctggcccgg gcatagccgg
      841 cggccatgaa acccgccccc tgctcgttag ccgggacgat gagagggatc tgcggttctc
      901 ccaggcggcc ggcggcctcg ttgtagagga acacggcgtc gtaggtgggc aggatcgccc
      961 cgccgctgta gccgaagatc gccgtcacgc cctcatcggc cagcacctgc atgatcatgc
     1021 gggcgccgct catgcgctcg cccgcgaggg ggtggcccgc cggacgcggc tggatcactg
     1081 tgtcggtcat ggacgcttct tggctccgaa cgcccgccgt ccgggaaaaa agtcgaaggg
     1141 atcaacgagc ctagcagctt gccctcatgg tgcaaactcg caccgatcct ttattctgca
     1201 gggcctttcc ctagtcccgc cttaaccata atagccttgc atgcgccaca tcatcgccat
     1261 cctgctgcag aacgaagccg gcgccctgac gcgcgtcgcg gggctcttct ccacacgcgg
     1321 ctacaacatc gaatccctct cggtcgcccc gaccgacgac cccacggtct cgcgcctgac
     1381 cctggtaacg cgcggctccg attcggtcat ccagcagatc gccaaccagc tgaacaagct
     1441 cgtcgacgtg gtgaacgtgc tcgacatgac gcgcggggag cacctcgagc gcgagctcgt
     1501 actgcttaag cttaaggtcg aggcggggca ggcggatgcg gtgcgtggcc aggtcgtgcg
     1561 cggcgggggc cgggtgctgg accccgcccc ggacggcttc atcgtcgaac tgacggcaag
     1621 tgaggccgaa ataaacggct tcgtcgccga tcttggcagc cgggcggaac tggtggaagt
     1681 ggtgcgcagc ggcgcgctgg gtatcagccg caacgcccgt ccgctgcggg cccgcccggc
     1741 accggccctc ctctagggcc ccacttacgg tcgcagcagc gtcagctgcc acacttcctt
     1801 gccggtggcc atcatcagcg ggcgcagggc gaggaccccg gccttgccga tggcgcgctc
     1861 gaggtgcgcg aactcggtga ggcggtcacg cgtctccgcc tcgatcggca gctcgggcag
     1921 gccggcttcg cgcatcagga gcaccccctt ggcgcgcacg gcgagttcgc cgtgcacccg
     1981 caggtagcag agcatgtcgg ccagttcctc gccgcgaaag cgatcgcgca gtgcctgcag
     2041 atagcggccg gagttgctgc cgaggaacgt gccggcgctg atcgactcca gcagcttcac
     2101 gtccgactcg agatccacct cgatccaatt gcgcagaatc cgctcactgt gatcgaacac
     2161 caggtagatc acgaacggca acaccagcag ggtcgcgagc gtcgccagcg ccggccttac
     2221 cagcaggcag ttgaagccgg tgtgcagcgc gaccgccgcc agaaagccgg gcagtaacag
     2281 atacaggcca ccggacgggc gccgctcgta gagcgtgttc gagatcatcg ccagcaccgc
     2341 cgcagttccg ccatgcatga tcgccgtacc gaaaccgcgg atcactgcga cgggcagcgc
     2401 ggtgtgcgac tgcgacatga ggtagtagat gttctcgacc agcgcgaagc cggtaccgat
     2461 ggcgaagcct gcgatggcgg cgtccaccgg caagcccacg cgccgcgtgc ggatgaggaa
     2521 cacgagcaac gccgccttca gggcttcctc gatccagggc gatacgtagc gcgaataggc
     2581 ggcaaaacct cccgcgaaat gcgcgtacgc tgcgttgttg agcaggtaac tggcgcccgc
     2641 cgcaagcgcc ccggccgcga ccagggccag ca
//
LOCUS       DATNBV010000423         2166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_255150,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000423 DATNBV010000000
VERSION     DATNBV010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255150"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VN627_10085"
     CDS             <1..688
                     /locus_tag="VN627_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HXD03643.1"
                     /translation="GQDRDSLTASLDEAIGLEPDRIALFGYAHVPHLLPRQRRISAEA
                     LPDQAERFVMAALGHEQLVAAVYVPVGFDHFALPHDPLAKAALEGRLHRNFQGFTDDD
                     GALIGVGASAISQFPDLIVQNEKNSGRYRMRAIAGRTSGERGVWRSAEDQRRGAAIEA
                     LLCGRAADIGTLEETVEVQLAPFLDRGLARLSEKRLWVTADGLPYARTIAALFDGYRA
                     VSPQRFSTAI"
     gene            691..1389
                     /locus_tag="VN627_10090"
     CDS             691..1389
                     /locus_tag="VN627_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXD03644.1"
                     /translation="MDAPAVSLIGGVMLGLASSLHCIGMCGGISLLLGSPAIGRPSAL
                     FLADQLALQGGRVLSYAALGGLAGGIGGAALGGLDAASGHALLRWAAALSFGWVGLSL
                     AGLMPSPAILGRLLPLRRIGAVTGWRVPGPVRGIASGLVWGLLPCGMVYGALLFALFA
                     GSAAGGAVVMLGFGLGTLPALIGAGWGIGALRPRIGERSFGLILVLIAAASMVEPEAG
                     IGRLCRAAGRLFLF"
     gene            1488..1793
                     /locus_tag="VN627_10095"
     CDS             1488..1793
                     /locus_tag="VN627_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03645.1"
                     /translation="MPGTERCSWGWVFMVAFHEGLRRDLPVRLLAGSSRKASITLARE
                     LLGVVRELEESSAEKPVDQHPPCPSCGGHMIIIETFERWRQPRPPPTAATYDRELAP"
BASE COUNT          308 a          738 c          720 g          400 t
ORIGIN      
        1 cgggcaggac agggacagct tgacggcgtc gctggacgag gccatcgggc tcgaaccgga
       61 ccggatcgcg ctcttcggct atgcgcatgt tccgcatctg ctgccgcgcc agcgccggat
      121 cagtgccgaa gcgctacccg atcaggcgga gcgattcgtc atggcggcgc tcggccatga
      181 acaactggtt gcagccgtct atgtccccgt tggtttcgat catttcgcgc tgccgcacga
      241 tccgctggcc aaggccgcac tggagggcag gctgcaccgc aatttccagg gctttaccga
      301 cgatgacggc gccctgatcg gcgtcggcgc cagtgcgatc agccagtttc cggacctcat
      361 cgtacagaat gaaaagaata gcggccgcta ccgcatgcgt gcgatcgcgg gccgcacgtc
      421 cggcgaacgc ggcgtatggc gctcggctga ggatcagcgg cggggcgcgg ctatcgaagc
      481 gctgctgtgc ggtcgcgcag cggatatcgg cacccttgag gagacggtgg aagtgcaact
      541 cgcgcctttt cttgatcgcg gtctggcgcg tctcagcgag aagcggcttt gggtaacggc
      601 ggacggcctg ccctatgccc ggacgattgc cgccctgttc gacggttatc gcgccgtctc
      661 gccgcaacgg ttcagcaccg ccatctgacc atggacgcgc ctgctgtcag cctgatcggc
      721 ggcgtgatgc tgggccttgc ctcgagcctg cactgtatcg gcatgtgcgg cggtatttcg
      781 ctactgctcg gctcgcccgc aattgggaga ccctccgccc tcttcctggc cgatcagctc
      841 gcgctgcagg gcgggcgtgt gctcagctat gccgcgctgg gtgggctggc gggcgggatc
      901 ggcggcgcag cgcttggcgg gctcgatgcg gcgagcggcc atgccctgct acgctgggcg
      961 gcagccctgt cctttggctg ggtcggcctg tcgcttgccg ggctgatgcc gtcgcctgca
     1021 attcttggcc gcctcctgcc gctgcggcgg attggcgcgg taacgggctg gcgggtgccg
     1081 gggcctgtcc ggggcattgc cagcggtctt gtgtgggggc tgttgccctg tggcatggtc
     1141 tatggcgcgc tcctctttgc cctctttgcc ggcagtgcgg cgggcggtgc ggtggtcatg
     1201 ctgggcttcg ggcttggcac cctgccggcg ttgatcgggg cagggtgggg catcggggcg
     1261 ctcaggccaa ggatcggcga gcggagcttc ggcctcatcc tggtcctgat cgcggccgcc
     1321 agcatggtcg aaccggaagc ggggatcggc cggctgtgcc gcgcggccgg ccgcctcttc
     1381 ctgttctgac cggcctcctc tgcgaccgga tattgtcgcg catgctctgg ttcgggttga
     1441 tcggcacgcc aacgccgccg gtgtcgttgc catagcgggc attgtagatg cccggcaccg
     1501 agcgctgcag ctgggggtgg gttttcatgg tagccttcca tgaaggcctc cgtcgcgatc
     1561 ttccagtccg gctcctcgcc ggctcctcgc gcaaggccag catcacgctc gcccgcgaac
     1621 tgctgggcgt cgtgcgcgag ctcgaggaat ccagcgccga gaaaccggtc gaccagcatc
     1681 cgccatgccc aagctgcggt gggcatatga tcatcatcga gacgttcgag cgctggcgac
     1741 agcctcgccc tccaccgact gcagcgacgt acgaccggga gcttgcgcca tgacccggcg
     1801 tggcccgctc atactccatc ccgttgcgac gctgcttcgg gaaaccacgc ccgctgtgcc
     1861 gaaggtcacc tgcatcgcca aaatcccgtt cgtcgcggcg cactcacccc gctcagaccg
     1921 gccaacacgc agcggtcaga actcatcccg ccacgagcaa tcgcacccag cgatcatcgt
     1981 accgttccca atcaccagcc aaatccgcaa tccccataga ccatcgcctg gggctccgcg
     2041 ggtcgtacac gtcagggttt agtacgcctc ccggcgcccg aaaccctcag acctttcagt
     2101 catcctccag tcgccccaat cccctcgtcg cccaacgaaa aggccggcga gaggggtgcc
     2161 cccgcc
//
LOCUS       DATNBV010000424         3231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2161954,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000424 DATNBV010000000
VERSION     DATNBV010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161954"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VN627_10100"
     CDS             <1..751
                     /locus_tag="VN627_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXD03646.1"
                     /translation="TLDMLRDFGSMSDKMCTALKIAGACRMNVVISGGTGSGKTTMLN
                     ALSKMIDPGERVLTIEDAAELRLQQPHWLPLETRPPNLEGQGAITIGDLVKNALRMRP
                     DRIILGEIRGAECFDLLAAMNTGHDGSMCTLHANSPRECLGRMENMILMGDIKIPKEA
                     ISRQIAESVDLIVQVKRLRDGSRRTTNITEVIGMEGDVIVTQELFKFEYLDESDDGKI
                     IGEFRSTGLRPYTLEKARQFGFDQAYLEACL"
     gene            822..1733
                     /locus_tag="VN627_10105"
     CDS             822..1733
                     /locus_tag="VN627_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXD03647.1"
                     /translation="MIAPNRPFLALGLRLLSGAAFSAMLLLVKLSGKSGVSLPEIMFW
                     RQCLPAVGLIASLGLTGRMHLVRTQRPGLHARRSIIGTSNMFLTLRAVQLMPLAEATV
                     LSFTAPVFAVILAVVMLGERVGRWRLGALVLGLAGVVVIAGPDHGLISPLALIVGLGA
                     AFGVALVSIQVRQMSATEAPITVVFWFSLVGTVLLLPLLLIYGKPHSLAQWATLAGVG
                     ICGLAGQLLMTGSLRHGSVSSVIVMDYAQLGWATVWGWLVFDQLPPAMTWVGGPLIIA
                     AGMIIARREQLLHRRPALDPQTGPNAD"
     gene            complement(1752..>3231)
                     /gene="gcvPB"
                     /locus_tag="VN627_10110"
     CDS             complement(1752..>3231)
                     /gene="gcvPB"
                     /locus_tag="VN627_10110"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HXD03648.1"
                     /translation="RALMLEEPLLFEIGKTDQTGVDFADLPTRATRLGGLERSRAIGL
                     PGLSEPETVRHYTRLSRQNYAIDLGLFPLGSCTMKHNPRLNEKVARLPGFADVHPLQP
                     QETVQGALQVINELAFWLLDLTGMYGVAMSPKAGAHGELCGLLCIRAALEARGDKREV
                     VLVPESAHGTNPATAAFAGYRVENIPANAAGRVNLEALKARLGPDVAAVMITNPNTCG
                     LFEPDMKAISDAVHAAGAFVYCDGANFNAIVGKVRPGDLGIDAMHINLHKTFSTPHGG
                     GGPGSGPVVLSEALSPYGPLPFAAKYPDGTYKIVEEETAGEEHPNAFGRMVAFHGQMG
                     MFTRAASYMLSHGADGLRQVSGDAVLNANYILRQLEDVLDAPFAASGPCMHEALFSDN
                     GMAPGFTTLDIAKGLIDEGFHPMTVYFPLVVHGAMLVEPTETESKAGLDRFIASMRSL
                     AARARNADPSLKSAPHLAPRRRLDETLAARRPVLTWHGNAAD"
BASE COUNT          520 a         1037 c         1097 g          577 t
ORIGIN      
        1 cacgctggat atgctgcgcg acttcggttc gatgtccgac aaaatgtgca cggcgctcaa
       61 gatcgcgggc gcctgccgga tgaacgtggt catctccggc ggtaccggtt cgggtaagac
      121 gaccatgctc aacgcgctgt cgaagatgat cgacccgggc gagcgcgtgc tgacgatcga
      181 ggacgcggcc gaacttcgcc tgcagcagcc ccactggctg ccgctcgaaa cccgcccgcc
      241 gaaccttgag ggccaaggcg cgatcaccat cggcgacctc gtcaagaacg ccctgcgtat
      301 gcgtcctgac cggatcatcc tcggcgaaat ccgcggcgcg gagtgtttcg accttctcgc
      361 cgcgatgaac accggccacg acggttcgat gtgcacgctc cacgccaact cgccgcgtga
      421 gtgtctcggc cgtatggaaa acatgatcct gatgggcgac atcaagatcc ccaaggaagc
      481 cattagccgc cagatcgccg aatcggtcga cctgatcgtc caggtcaagc gcctgcgcga
      541 cggcagccgc cgcaccacga acatcaccga ggtgatcggc atggaaggcg acgtgatcgt
      601 cacgcaagag ttgttcaagt tcgagtatct cgatgagagc gacgacggca agatcatcgg
      661 cgaattccgc tcgaccggcc tgcgccccta cacgctggaa aaggcgcgtc agttcggctt
      721 tgaccaggct tatctggagg cctgcctcta gcgaccgtgc tggtcccgct ccccgggacc
      781 attgcacttg cctcgcccgc aagcgtggct aaagccccgg tgtgattgca cccaaccgtc
      841 cgtttcttgc tcttggcctg cgcctgctgt ccggcgcggc cttttccgcg atgctgctgc
      901 tggtcaagct gagtggcaag agcggggtgt ctctgcccga gatcatgttt tggcggcagt
      961 gcctgccggc cgtcggcctg atcgcatcgc tgggcctgac cggccggatg cacctggtcc
     1021 gcacgcagcg tcccggactc catgcccgcc gttcgataat cggtacgagc aacatgttcc
     1081 tcaccctcag agcggtgcaa ctgatgccgc ttgccgaggc gaccgtcctg agcttcaccg
     1141 ctccggtctt cgcggtcatc cttgctgtcg tcatgcttgg cgaacgggtc ggccgatggc
     1201 gcctgggggc gctggtgctg ggcctggccg gcgtagtggt gattgccggg ccggaccacg
     1261 ggctgatcag cccgcttgcc ctgatcgtcg ggctgggcgc ggctttcggc gtggcgctgg
     1321 tttccatcca ggtccgccag atgtccgcga cggaggcgcc gatcacggtg gtattctggt
     1381 tctcgctggt cggcaccgtg ctgctgttgc cgctgctgct gatttacgga aagccccaca
     1441 gcctggcaca atgggcaact cttgccgggg tgggcatctg cggcctcgcg gggcagctgc
     1501 tgatgaccgg ctcgctacgt catggatcgg tctcgagcgt gatcgtgatg gactatgccc
     1561 agctcggctg ggcgacggtg tggggctggc tggtcttcga ccagctgccc ccggccatga
     1621 cctgggtcgg cggcccgctc atcatcgcgg cgggcatgat catcgcgcgg cgcgaacagc
     1681 tgctccaccg ccggccggcg ctcgacccgc agaccgggcc gaacgccgac tgagcggccg
     1741 acgcgcgggg ttcagtcggc cgcatttcca tgccaggtca gcaccggcct gcgcgcggcc
     1801 aaggtctcgt caagccggcg ccgcggcgcc aggtgcggcg cggacttgag gctgggatca
     1861 gcgttgcgcg cacgggcggc gaggctgcgc atggaggcaa tgaaccggtc gagcccggcc
     1921 ttgctctcgg tctcggtcgg ttcgaccagc atggcgccgt ggaccaccag cgggaaatag
     1981 accgtcatcg ggtggaagcc ctcgtcgatc aggcccttgg cgatatccag cgtggtgaag
     2041 ccgggcgcca tgccgttatc ggagaagagc gcctcatgca tgcacgggcc ggaggcggcg
     2101 aagggtgcgt cgagcacatc ctccagctgg cgcaggatgt aattggcgtt gagcacggcg
     2161 tcgcccgaga cctggcgcag gccgtcggcg ccgtggctga gcatgtagga ggcggcgcgg
     2221 gtgaacatgc ccatctggcc gtggaaggcg accatgcggc cgaaggcgtt gggatgctcc
     2281 tcgcccgccg tttcctcctc gacgatctta tacgttccat cgggatactt cgcagcgaac
     2341 ggcagcgggc cgtaaggcga tagcgcttcg gacaggacga ccgggcccga acccgggccg
     2401 ccgccgccgt gcggggtcga gaaagtcttg tggaggttga tgtgcatcgc atcgatgccg
     2461 aggtcgcccg gccgcacctt gccgacgatc gcgttgaagt tggcgccatc gcaatagacg
     2521 aaagctccgg cggcatggac cgcgtcgctg atcgccttca tgtcgggctc gaacaggccg
     2581 caggtgttcg gattggtgat catcaccgcc gcgacgtcgg gtccgagccg agccttcagg
     2641 gcttcgagat tgacgcggcc ggcggcgttc gccgggatat tctcgacacg atagccggca
     2701 aaggccgctg tcgccgggtt tgtgccgtgc gcactttccg gaaccagtac gacttcgcgc
     2761 ttgtcgccgc gtgcttcgag cgcagcgcgg atgcacagca ggccgcaaag ctcgccatga
     2821 gcaccggcct tcgggctcat cgcgacgcca tacatgccgg tgaggtcgag gagccagaac
     2881 gcgagctcgt tgatgacctg gagcgcgccc tggacggttt cctgcggttg cagcggatgg
     2941 acgtctgcaa atcccggaag gcgcgcaacc ttctcattga ggcgcgggtt gtgcttcatg
     3001 gtgcacgagc cgagcgggaa gagtccgagg tcgatcgcgt aattctgacg cgagagccgc
     3061 gtgtagtgac gcacagtttc cggctcggag agcccgggca atccgatggc acgcgagcgt
     3121 tcgaggccgc caaggcgcgt tgcgcgtgtg ggcaagtccg cgaaatccac gccggtctgg
     3181 tcagtcttgc cgatctcgaa cagcaacggc tcctcgagca tcagggcgcg g
//
LOCUS       DATNBV010000425         3693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1223421,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000425 DATNBV010000000
VERSION     DATNBV010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223421"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1043
                     /locus_tag="VN627_10115"
     CDS             228..1043
                     /locus_tag="VN627_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXD03649.1"
                     /translation="MLLCLLLLGACARVAAPQHPLADPTGGIASINPCSDAILAEVAD
                     PAQIAALSAYSRNPASSSMDVALARRLPVTSGTVEELAALGPSLVVSGTFTPPATRNA
                     LARLGIPLVELPIAGSVAQSEAQVRELARMAGHPERGEAMVARIEAALAAAKPPPGLP
                     VSAVVWQSGGIVPGNTTLIADLLRRTGFASFSAARGLRQADYLPLERMLADPPRVILA
                     AGGHGEEDRLLAHPALAALTHTSRAPLDPSLLWCGGPTIIRAAGRLAEIRRSL"
     gene            1040..2041
                     /locus_tag="VN627_10120"
     CDS             1040..2041
                     /locus_tag="VN627_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXD03650.1"
                     /translation="MTRLNLTLLAALVLALPLSLLAGRIWLDPFHPAAPNATVILLQL
                     RLPRALLAVTLGAGLGAAGAAMQGYLRNPLADPGLFGIAPSAALGAVLALWTGAAAGW
                     TLPLFALTGAGLAMALLGLIAGSHADGHSGGGGSGMALFTLAGLMIASLSGALMSLAI
                     SLAPTPFALSEIVTWLMGALTDRSWADLALAAPLTFIGLIVLRLAGPGLDALVLGEAA
                     ARSLGLDPRRLQGLLIAGIGLTVGAGVAVAGIIGFVGLVVPHLVRPFTDRRPSRLIVP
                     SALAGALLLLAADCLCRLLPLVTELRLGIALSLVGAPFFLALLLRLRRGTAPDAGLA"
     gene            2041..2802
                     /locus_tag="VN627_10125"
     CDS             2041..2802
                     /locus_tag="VN627_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXD03651.1"
                     /translation="MLRADNLVLPGRLCGVGLSLRPGEVTAIVGPNGAGKSSLVACLA
                     GLLRPAAGAVLLGDEPLASLPPRRRARQIGYLPQNPEIAWDITVETLVALGRLPWQGA
                     ALHRAHATPADDAAAIAAALAAMDLEPFRHRPVSRLSGGERARALAARVLAGQPRWLI
                     ADEPLANLDLAHAAALIALLRRQAHAEGRGVVVVLHDLAMAMNHADRVIVLAQGQVVA
                     DGPPATALTGTLVTRVWGLPARWLGEPGTSALALG"
     gene            complement(2814..>3693)
                     /locus_tag="VN627_10130"
     CDS             complement(2814..>3693)
                     /locus_tag="VN627_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXD03652.1"
                     /translation="EIVVALDADTLFPADTLRHLVAPLADPTVGAVAGNAKVGNRINL
                     LTRWQAIEYITSQNLDRRAFSLLNCITVVPGAVGAWRKSLVLEVGGFSTDTLAEDQDL
                     TIAIQRKGWKVAYDEQAVAWTEAPENFGALAKQRFRWSFGTLQCLWKHRAVLRERQPG
                     GLAFVGMPQAWLFQIAFAVVSPLIDLALAVSIGGTLLRVYEHGWAQTQTDVLRMAAYW
                     AGFVAIDLACGLVAFRLDVRGEKFRPLLLLAQRFVYRQVMYSVVIRAVSAALHGMAQG
                     WGKLERTGRVATPVGS"
BASE COUNT          441 a         1437 c         1233 g          582 t
ORIGIN      
        1 ctgcgcgcca gtctgccggt caccgataag atcgagctct atggccgtgt cgagaacgtc
       61 accgacgcca ggtaccagac cgtcgccggc tacaacacgc cgggccgttc ggcctatgcc
      121 ggcataaggg cgcggttctg acgctccgct tcgcctctgc tgtcgccgcg gctcatcccg
      181 cggcgaacag ggggaccgcc ccgcgccggc cgggacggtg gagcggagtg ctgctttgcc
      241 tcctgctgct gggagcctgc gcaagggtcg cagccccgca gcaccctctg gccgacccga
      301 cggggggcat cgccagcatc aatccctgca gcgatgcgat cctcgccgaa gtggccgatc
      361 cggcccagat cgccgccctc tcggcctata gccgcaatcc ggcgtccagt tcgatggacg
      421 tggccctggc tcgccgcctg cccgtcactt caggcaccgt cgaggagctc gccgctctcg
      481 ggccttcgct cgtcgtctcc ggcaccttca cgcccccggc aacccgcaat gcgctggccc
      541 ggcttggtat tccgctggtc gaactgccca tcgccggcag cgtcgcacag agcgaggccc
      601 aggtccgcga gcttgcccgg atggccggcc acccggagcg gggcgaggcc atggtcgccc
      661 ggatcgaagc cgcgcttgcc gccgccaaac cgccgccggg ccttcccgtc agcgcggtgg
      721 tctggcaatc gggcgggatc gtgccgggga acaccacgct catcgccgac ctgctgcgcc
      781 gcaccggctt tgccagcttc agcgccgcgc gggggctgcg ccaggccgat tacctgccgc
      841 ttgagcgcat gttggccgat ccgcctcgcg tgatcctcgc cgccggcggg catggcgagg
      901 aagatcgcct gctggcccat cccgcgctcg ccgcgcttac ccacaccagc cgggccccgc
      961 ttgatccctc gttgctgtgg tgcggcggcc cgaccatcat tcgcgccgcc gggcgcctcg
     1021 ccgaaatcag gcgcagcctg tgacccggct caacctcacg ctgctcgccg ccctcgtgct
     1081 ggcgctcccc ctttcgctcc tcgccgggcg catctggctc gatccattcc atccggctgc
     1141 gcccaacgcc accgtgatcc tgctccagct gcgcctgccc cgcgccctgc tcgcggtcac
     1201 gctcggcgcc ggcctcggcg cagcgggcgc ggccatgcag ggctacctgc gcaacccgct
     1261 ggccgatccc ggcctgttcg gcatcgcccc ttccgccgcg ctgggcgccg tcctcgccct
     1321 gtggaccggt gccgccgccg gctggaccct cccgctgttt gccctcaccg gcgcaggact
     1381 cgccatggcc ctgctcggcc tgattgccgg gagccatgcc gatgggcaca gcggcggtgg
     1441 cggctccggc atggccctgt tcacgctcgc cggcctgatg atcgccagcc tctccggcgc
     1501 gctgatgagc ctggccatca gcctcgcccc cacgcccttc gccctgtccg aaatcgtcac
     1561 ctggctgatg ggcgcactga ccgaccggtc atgggccgac ctggccctcg ccgctccgct
     1621 cacgttcatc ggcctgatcg tcctgcgcct cgccggcccg ggcctcgatg cgctggtgct
     1681 gggcgaggcc gcggcccgtt cgctcggcct cgatccgcga cggctgcagg ggctcctgat
     1741 tgcggggatc gggctgaccg tcggcgcggg tgtcgccgtc gccgggatca tcggcttcgt
     1801 cggcctggtc gtcccccatc tggtccgccc gttcaccgac cggcgcccct cgcgcctgat
     1861 cgtcccctcg gcgctcgccg gggcgctgct gctgctggcc gccgattgcc tgtgccgcct
     1921 gcttccgctg gtcaccgaac tgcgcctcgg catcgccttg agcctcgtcg gcgcgccgtt
     1981 cttcctggcc ctgctgctgc ggctgcgccg tggcaccgcg cccgacgcgg gcctcgcctg
     2041 atgctgcgcg cggacaatct cgttcttccc ggccgcttgt gcggcgtggg cctcagcctg
     2101 cggcccggcg aggttaccgc catcgtcggg cccaatgggg cgggcaagtc cagcctcgtc
     2161 gcctgcctcg ccggcctgct ccgcccggct gccggtgccg tcctgcttgg cgacgagcca
     2221 ctggcctcac tcccgccgcg ccgccgcgcc cggcagatcg gctacctgcc gcaaaatccg
     2281 gagatcgcct gggacatcac ggtcgaaacg ctggtcgccc tcggccgcct gccgtggcag
     2341 ggcgccgcgc ttcaccgcgc ccatgccaca ccggccgacg acgccgccgc cattgccgct
     2401 gccctggcgg cgatggacct ggagcccttc cgccaccgcc cggtctcacg cctgtccggc
     2461 ggcgaacgcg cccgtgcctt ggcggcacgc gtgcttgccg gccagccgcg ctggctgatc
     2521 gccgacgagc cacttgccaa cctcgacctg gctcatgccg ccgcgttgat cgccctgctg
     2581 cgccgtcagg ctcatgccga gggccgcggc gtcgtcgtgg tcctccacga tctcgccatg
     2641 gcgatgaacc atgccgaccg ggtgatagtt ctcgcacagg gccaggtcgt cgccgatggc
     2701 ccgcccgcca ccgcgctcac cggcacactc gtcacccgcg tctggggcct gcccgcgcgc
     2761 tggcttggcg agccgggcac aagcgccctg gcgctcggct agggtttcgc cggtcagcta
     2821 cccaccggcg tggcgacccg gccggtccgc tcgagcttgc cccagccctg ggccatgccg
     2881 tggagcgccg cgctgaccgc gcggatcacc accgaataca tcacctgccg gtagacgaag
     2941 cgctgcgcca gcagcagcag cgggcggaac ttttcgcccc gcacgtcgag ccggaaggcg
     3001 accagcccgc aggcgaggtc gatggcgacg aagcccgccc agtaggcggc catgcgcagc
     3061 acgtcggtct gggtctgggc ccagccgtgc tcatagaccc gaagcagggt cccgccgatg
     3121 gagacggcaa gggccaggtc gatcagcggg gagaccacgg caaaggcgat ctggaacagc
     3181 caggcttgcg gcatgccgac gaaggcgagg cccccgggct ggcgttcgcg caggacggcg
     3241 cggtgcttcc acaggcactg gagcgtgccg aacgaccagc ggaaccgctg cttggcgagc
     3301 gcgccgaagt tttcgggcgc ttcggtccag gctacggcct gttcgtcata ggcgaccttc
     3361 caacccttgc gctggatggc gattgtcagg tcctggtcct cggcgagggt gtcggtgctg
     3421 aacccgccga cctcgaggac gagggacttc cgccacgcac cgaccgcgcc gggcaccacc
     3481 gtgatgcagt tgaggaggga gaaggcgcgc cggtcgaggt tctggctggt gatgtactcg
     3541 atcgcctgcc accgtgtgag gaggttgatc ctgttcccca ccttggcgtt ccccgcgacg
     3601 gcgcccaccg tgggatcggc caggggcgcc accaggtggc gcagcgtgtc ggcggggaag
     3661 agggtgtcgg cgtcgagcgc gacgacgatc tcc
//
LOCUS       DATNBV010000426         6117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1821197,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000426 DATNBV010000000
VERSION     DATNBV010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821197"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VN627_10135"
     CDS             complement(<1..620)
                     /locus_tag="VN627_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HXD03653.1"
                     /translation="MHAAERERLIEEALRPTGFVSYRELEALLEASPATIRRDLTRLE
                     ETGRIVRVHGGARRAEEADGSPRLIGTPFDLSISQNLAAKQAIGRAAAALVQPGEGIM
                     VDGGTTTLQMCPHLAGRHCQVLTNSLHIVNALLPQDGTRILVPSGTVFREQNIILAAA
                     GEDSMPRFHAPKLFMGAAAVGPRGIMQADVILVAAERRLIDRAEEVV"
     gene            complement(648..1919)
                     /locus_tag="VN627_10140"
     CDS             complement(648..1919)
                     /locus_tag="VN627_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD03654.1"
                     /translation="MTTPVHADTMDRREHWRNTILAGLANYIDAGSIVSGSVALALWK
                     DLYHLSDSFIGLIGAFSANAISAGVGALIGGRLCDLFGRKKIYQFDMLFYAFGMLFLI
                     FAAAPWMIITGFLLVGLAVGADIPASWSLIAEQAPDHERGAHSGVAQILWYLGPVAVL
                     VAGYFLKPFGIGGVRWLFIHLAVLALALTFLRSRMRESERWEETVATAESTGKLPGWS
                     DVLTPRHLGAILYLVTMYGMWNLWAGYNGFFTPYLIEQNHLPQWAATVVPASYFFVGM
                     LSIWFVFMRLSDKVNQRTLFAVSAVMHVAGMALLAVFGFKLEVVIIHILIMGVAGGFG
                     AQSFFQLWSAELFPTAIRSTAQGLAFAVVRIGLGIWSLAVPSLAASNFTELAWILTGF
                     LVVSGVVGFVWAPRNEGKSLEQLEAERQGGA"
     gene            complement(1919..3067)
                     /gene="lldD"
                     /locus_tag="VN627_10145"
     CDS             complement(1919..3067)
                     /gene="lldD"
                     /locus_tag="VN627_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004457 - lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HXD03655.1"
                     /translation="MITASVADFREAARRRLPRFLFEYIDGGSYAEQTLRRNVGDLAD
                     IALRQRVLRDVSQLDLSTQLFGQKLALPVALAPIGLAGLNARRGERQAVRAAERAGVP
                     FTLSTVGACPIGEVAAAATKPFWFQLYMIRDRAFMRDLLAQARAAGCSTLVFTVDMPV
                     PGSRYRDHHTGLAGAPGLKGAVWRAAQAALRPGWAWDVGLRGRPHTLGNVAPVLQGRT
                     GIEDFFAWMRNNFDPAINWRDLDFIRSEWTGPLVIKGILDPDDAREAAELGADGIVVS
                     NHGGRQLDGVLSSARALPPIAEAVGDRLTVLADGGVRSGLDVVRMLALGAKGVLLGRA
                     WAWALAAGGEAGIVKMLGIIEAEMRIAMALTGVTRIDEINPEILAGRA"
     gene            complement(3064..4467)
                     /locus_tag="VN627_10150"
     CDS             complement(3064..4467)
                     /locus_tag="VN627_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HXD03656.1"
                     /translation="MSTGATIVIDLGKTLSKVSLWDFAGLMLAREVRPNLPFAVDGIR
                     RLDTRGIGEWLVETLAKFASHPVRRIIPVGHGAGVVAIGPHGPSFPPLDYEQPIPPSV
                     LAAYRAERDPFALTGSPALPDGLNIGSQLYWAERLYPGVMDTATLVPWAQYWAWFLTG
                     RAVSEVTSLGCHSDLWCPADGTFSPMARRLGWAERFAPVVPAGEVVGTLQPDISRRTG
                     LSADVEVLAGLHDSNAALLAARGFPEIARNEATVLSTGTWFIALRTPAGPVDTASLPE
                     ARDCLVNVDAYRQPVPSARFMGGREIETLIEIDTRRIDIKPDQPALLAAVPEVLATEA
                     MILPTLAPGCGPFPDGRFDFLNRPDDWYQRRAAACLYAALVADASLDLIGSKERVLIE
                     GRFAEAEVFVRALAALRPGTAIFTANAHNDVSFGALRLIDPALTPAGELVRVTPLEQD
                     LGPYRARWAAHVKALAA"
     gene            4587..>6117
                     /locus_tag="VN627_10155"
     CDS             4587..>6117
                     /locus_tag="VN627_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HXD03657.1"
                     /translation="MNAQAPISAAALSFAVPASRWDDSVAARLSPAELLLYRSNLLGS
                     DLTVTNFGGGNTSAKLMESDPLTGAEVEVLWVKGSGGDIGSMKLDGFATLYQDKLLSL
                     EAHYAGPEDDDKMVGYLPHCTFNLNSRAASIDTPLHSLLPFAHVDHVHPDAIIALAAS
                     SGGEAATKAIWGGKIGWLGWKRPGYTLGVMLRDYVAANPGIEGVMLAGHGIICWADSA
                     KACYEHTVQLIADAANYLNAKLGDKPAFGGQKVAPNPDRAAIAADLMPRLRGFMTGAR
                     RKVGHYSDDAEALEFVGSAEFERLAALGTSCPDHFLRTKIAPLTLDPARLGDDAYLAE
                     KIADYRAMYAAYYERCKRGNSPAMRDSNPVVVLVPGVGRITFATDKTTARLAGEFYGN
                     AINVMRGAEAIGSYIALDEQEAFDIEYWLLEEAKLQRMPGPKPMVGRIALVTGGAGGI
                     GAATAARMLREGACVMLADRDASVVQEVCNGFAKQYGKDVVRSVVCDVTDEAQVANAF
                     AA"
BASE COUNT         1073 a         1991 c         2136 g          917 t
ORIGIN      
        1 acgacctctt cggcgcggtc gatcagccgg cgctcggcgg cgacgaggat cacgtccgcc
       61 tgcatgatcc cccgcggacc cacggcagct gcgcccatga acagcttggg cgcatgaaag
      121 cgcggcatgg agtcctcgcc cgccgcggcc aggatgatgt tctgctcgcg aaacaccgtg
      181 cccgagggca cgaggatgcg cgtgccgtcc tgcggcagca gggcgttgac gatatgcagc
      241 gagttggtga gcacctggca gtgccggccg gcaaggtgcg ggcacatctg cagggtcgtc
      301 gtgcccccgt cgaccatgat cccttcgccc ggctggacca gtgccgcggc ggcccgaccg
      361 atggcctgct tggcggcaag gttctgactg atcgacaagt cgaacggcgt gccgatcagg
      421 cggggtgagc catcggcttc ttccgcccgc ctggccccgc catgaacgcg gacgatgcgg
      481 ccggtctcct ccagccgggt aagatcgcgg cggatcgtcg cgggggaggc ttcgagcagg
      541 gcctcaagtt cgcggtagct gacgaacccg gtgggccgca gggcttcctc gatcagccgt
      601 tcgcgttccg ccgcgtgcat ggtcttgccc tgcccttatc cgcctcgtca ggcaccccct
      661 tgccgctcag cttcgagctg ttcaagtgat ttgccctcat tgcgcggcgc ccagacgaag
      721 ccgaccacgc cgctgaccac gaggaagccg gtgagaatcc acgccagctc ggtgaaattg
      781 ctggccgcca gcgaaggcac ggcaaggctc cagatgccaa ggccgatgcg gaccacggcg
      841 aaggcgaggc cctgggcggt ggagcggatc gcggtgggga acagctcggc gctccacagc
      901 tggaagaagg actgcgcgcc aaagccgccc gccacgccca tgatcaggat gtggatgatc
      961 accacctcca gcttgaaccc gaacacggcc agcagggcca tgccggccac atgcatcacc
     1021 gccgagaccg cgaacagtgt acgctggttg accttgtccg acagcctcat gaagacaaac
     1081 cagatcgaga gcatgccgac gaagaagtag ctcgccggga ccacggtcgc cgcccactgg
     1141 ggcaggtggt tctgttcgat caggtagggc gtgaagaagc cgttgtagcc ggcccacagg
     1201 ttccacatgc cgtacatggt gacgagatag aggatcgccc cgaggtgccg cggcgtgagc
     1261 acgtctgacc agcccggcag cttgccggtg ctttccgccg tggcgacggt ctcctcccac
     1321 cgctccgatt cccgcatgcg tgaccgcagg aaggtgagcg cgagggcaag gaccgccaga
     1381 tgaatgaaca gccaccgtac cccgccgatg ccgaagggct tgaggaaata gccggccacc
     1441 agcacggcca ccggccccag gtaccacagg atttgcgcga cgccggaatg ggcgccgcgt
     1501 tcgtggtcgg gggcctgctc ggcgatgagc gaccagcttg ccgggatgtc ggcgcccacg
     1561 gcgaggccga ccagcaggaa cccggtgatg atcatccacg gcgcggcggc gaagatcagg
     1621 aacagcatgc cgaaggcgta gaacagcatg tcgaactggt agatcttctt gcgcccgaac
     1681 aggtcgcaca gccgcccccc gatcagcgcg ccgacgccgg ccgagatggc attggccgaa
     1741 aaggcgccga tcagcccgat gaagctgtcg gacaggtggt acaggtcctt ccacagcgcc
     1801 agggcaaccg agcccgacac aatcgagccg gcgtcgatgt aattggcaag gccggcaagg
     1861 atcgtgttcc gccagtgctc ccggcggtcc atggtatcgg cgtgaactgg tgtggtcatc
     1921 aggctctccc ggccagaatt tctggattga tttcgtcgat ccgggtgacc ccggtcagcg
     1981 ccatggcgat gcgcatttcg gcctcgatga tgccaagcat cttgacgatc cccgcctcgc
     2041 cgccggcggc gagcgcccag gcccaagcgc ggcctaacag gacgcccttt gcgccgagcg
     2101 ccagcatccg caccacgtcg agtccggagc gcacgccgcc atcggcgagc acggtcagcc
     2161 ggtcacccac ggcctcggca atggggggca aggcccgcgc gctggagagc acgccatcga
     2221 gctggcgccc gccgtggttg gagacgacga tcccgtcggc ccccagctcg gccgcctcgc
     2281 gcgcgtcgtc aggatccagg atgcccttga tcaccagcgg gccggtccat tccgaccgga
     2341 tgaagtcgag gtcgcgccag ttgattgcgg gatcgaagtt attgcgcatc caggcgaaga
     2401 aatcctcgat cccggtgcgg ccctggagga cgggggcgac gttgccaagc gtgtgggggc
     2461 gaccgcgcag gccgacgtcc caggcccagc cggggcgcag cgccgcctgg gcggcgcgcc
     2521 acaccgcgcc cttgagcccc ggcgcgccgg caaggccggt gtgatgatca cggtagcggc
     2581 tgccggggac cggcatgtcg acggtgaaca ccagcgtcga gcagcccgcc gcccgggcct
     2641 gggcgaggag gtcgcgcatg aaggcgcggt cgcggatcat gtagagctgg aaccagaaag
     2701 gcttggtggc ggcggcggca acttcgccga tcgggcaggc gccgaccgtg gaaagcgtga
     2761 aggggacgcc cgcgcgttcg gccgcgcgca ccgcctgccg ctcgccccgg cgggcgttga
     2821 ggccagcaag gccgataggg gcaagggcaa ccggcagggc cagcttctgg ccgaacagct
     2881 gcgtcgacag gtccagctgc gagacgtcgc gcaggacccg ctggcgcagg gcaatgtcgg
     2941 ccaggtcgcc gacattgcgg cgcagggtct gctcggcata ggagccgccg tcgatatatt
     3001 cgaacaggaa gcgcggcagg cgccgccggg cggcttcgcg gaaatcggcg accgaggcgg
     3061 taatcatgcg gcaagcgcct tgacgtgggc ggcccagcgg gcgcgatagg gaccgaggtc
     3121 ctgctccagc ggcgtgacgc ggacaagttc gccagcgggc gtgagcgcgg gatcgatcag
     3181 ccgcagggcc ccgaaggata cgtcgttatg ggcattggcg gtgaagatcg ccgtgcccgg
     3241 ccgcagcgcg gcgagggcgc gaacgaagac ctcggcctcg gcaaaccggc cctcgatgag
     3301 aacccgctcc ttgctgccga taaggtcgag cgaggcatcg gcgaccagcg cggcatagag
     3361 gcaggcggcg gcgcggcgct ggtaccagtc gtccgggcgg ttcaggaagt cgaaccggcc
     3421 atcggggaag gggccgcatc cgggggcaag cgtcggcagg atcatggctt cggtcgccag
     3481 cacctccggc acggcggcga gcagggccgg ctgatcgggc ttgatgtcga tccggcgggt
     3541 atcgatttcg atcagcgttt cgatctcgcg cccgcccatg aaccgggccg agggcaccgg
     3601 ctggcggtag gcatcgacat tgaccaggca atcgcgcgct tcgggcaggc tggcggtgtc
     3661 gaccggaccg gcgggggtgc gcagcgcgat gaaccaggtg ccggtcgaaa ggacggttgc
     3721 ctcgttgcgg gcaatttcgg gaaaaccgcg ggccgcgagc agggcggcgt tggaatcatg
     3781 aagtccggcg aggacttcga catcggccga gaggccggtg cgccgggaga tgtctggctg
     3841 aagcgtgccc accacctctc cggcgggcac caccggggcg aagcgttcgg cccagccgag
     3901 ccgccgggcc atcggcgaaa aggtgccgtc cgccgggcac cagaggtcgg aatggcagcc
     3961 gagactggtt acttcgctga ccgcccggcc ggtgaggaac cacgcccagt attgggccca
     4021 ggggaccagc gtcgccgtgt ccatcacgcc gggatagagg cgctcggccc agtacagctg
     4081 gctgccgata ttgagcccgt cgggcagggc gggtgagcca gtcagcgcga acggatcgcg
     4141 ctcggcccgg taggccgcca ggacagacgg ggggatcggc tgctcatagt ccagcggcgg
     4201 aaaggacggg ccatggggcc cgatggcgac tactcctgcg ccgtggccca ccgggatgat
     4261 ccggcggacc ggatggctgg caaatttcgc cagcgtctcc accagccatt cgccgatgcc
     4321 gcgcgtgtcg agccggcgaa tgccatcgac cgcaaagggg agattggggc gcacctcccg
     4381 cgcgagcatg agccctgcga agtcccacag gctgaccttg ctcagcgtct tgccgagatc
     4441 gatgacgatg gtggcgcccg tgctcaagcg cgtctctccc gcttcatagt gaaatgatca
     4501 ataccgatca ttcttgatca tttccagcaa aaacgataca cgctccccta gcaggcgcaa
     4561 ctgccgacaa atctccagag gacgcgatga acgcccaagc cccgatttcc gccgccgccc
     4621 tttcatttgc cgttccggcc agccgctggg acgactcggt ggcggcccgg ctctcgcctg
     4681 ccgaactgct gctctatcgt tcaaacctgc tcggctcgga cctgaccgtg acgaactttg
     4741 gcggaggcaa tacctcggcc aagctgatgg aatccgatcc gctgacgggt gccgaggtgg
     4801 aagtgctgtg ggtcaagggg tcgggcgggg atatcggctc gatgaagctg gatggcttcg
     4861 ccacgcttta ccaggacaag ctgctgagtc ttgaggcgca ttatgccggg ccggaagatg
     4921 acgacaaaat ggtgggttac ctgccgcatt gcacgtttaa cctgaacagc cgcgccgcct
     4981 cgatcgatac gccgctgcac tcgctgctgc ccttcgccca cgtcgatcat gtccaccccg
     5041 atgcgatcat cgcgttggcg gcctcatcgg gcggcgaagc ggcgacaaag gcaatctggg
     5101 gcggcaagat cggctggctg gggtggaagc gcccgggtta tacccttggc gtaatgctgc
     5161 gtgactatgt ggccgccaat ccggggatcg agggcgtgat gctcgccggc catgggatca
     5221 tctgctgggc cgacagcgcc aaggcctgct acgagcacac ggtgcaactt attgcggatg
     5281 cagccaatta cctgaatgcc aagctcggcg acaagccggc ctttggcggc cagaaggttg
     5341 ccccgaaccc cgaccgcgcg gccattgccg ccgacctgat gccccgcctg cgcggcttca
     5401 tgaccggcgc gcggcgcaag gtcggccact attccgacga tgccgaggcg ctggagtttg
     5461 ttggctcagc cgagttcgag cgcctggccg cgctcggcac ctcgtgcccc gatcacttcc
     5521 tgcgcaccaa gatcgccccg ctgacgctgg acccggcgcg gcttggggat gatgcctatc
     5581 tcgcggaaaa gattgccgat taccgcgcga tgtatgcggc ctactacgag cgctgcaagc
     5641 gcggcaattc gccggccatg cgcgattcca acccggtggt cgtgctggtg ccgggcgtgg
     5701 gtcgcatcac tttcgccacc gacaagacta cggcgcgcct tgccggcgag ttctacggca
     5761 acgccatcaa cgtgatgcgc ggggccgagg cgatcgggtc gtacatcgcg ctcgacgagc
     5821 aggaagcctt tgatatcgaa tactggttgc tcgaggaagc caagctccag cggatgcccg
     5881 ggccgaagcc catggtcggc cggatcgcgc tggtcaccgg aggggccggc gggatcggcg
     5941 cggcgacggc ggcgcggatg ctgcgcgagg gcgcctgcgt gatgctggcc gaccgcgatg
     6001 catcggtggt gcaagaggtc tgcaacgggt ttgccaagca atacggcaag gacgttgtgc
     6061 gctcggtcgt ctgcgacgtg accgacgagg cccaggtcgc caatgccttc gcggcaa
//
LOCUS       DATNBV010000427         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2806194,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000427 DATNBV010000000
VERSION     DATNBV010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2806194"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VN627_10160"
     CDS             <1..322
                     /locus_tag="VN627_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03658.1"
                     /translation="VERFNGRIGFEVLAITISSHGQLEQLLRGFNAAYNARRQRVLEA
                     KTPDQVVAERLAAKPALAKDKPSGQAGPCDITKARLIAENAKEVSQPDSSSLSDLTRR
                     IGLG"
     gene            371..1732
                     /locus_tag="VN627_10165"
     CDS             371..1732
                     /locus_tag="VN627_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HXD03659.1"
                     /translation="MKNFRAVDRDTGFLMPPSVDEWLPERHLARFVVEVVASLDLRAM
                     TGSYRGSGEASYHPAVLLGILVYGYATGVFSSRKLERATHDSVAFRFVAVNQHPDHDT
                     IAAFRRRFLKPIEALFVEVLQLAREMGVLKLGTIALDGTKIHANASRHSALSYEHAGK
                     IEAQLQAEVAELMAKAEAADQADVPDGMSIPEELARREQRLAEIARAKAIIEARAKER
                     HGREQAEYDATMAAREAKAAATGKKPGGRPPTPPVAEPGPTDQVNLTDEESRIMPVPG
                     GGFEQCYNAQAAVAAGSLLVVATDVVQASNDKQQVVPMLAKLDNLPDELGAIETLLAD
                     TGYFSEANVQACVAAEIDPLLAMGRQPHHPPLAERFAPAPPPPDNPTPVEAMAYRLKT
                     PEGRALYARRKHTPEPVFGIIKSALGFRQFLLRGLDNVRGEWNLVTMAYNVKRLFALA
                     AAA"
BASE COUNT          393 a          680 c          681 g          333 t
ORIGIN      
        1 ggtcgagcgc ttcaatggcc gcatcggctt cgaggtgctt gccatcacca tctcctcgca
       61 cggtcagctc gaacagcttc tgcgcggctt caacgccgcc tacaatgccc gtcgccagcg
      121 tgtgctggag gccaagacgc ccgatcaggt cgtggctgag cggctcgcag ccaagcccgc
      181 actggccaaa gacaagccat ccggacaggc cggtccttgc gatatcacta aggcccgcct
      241 gatcgctgaa aacgccaagg aggtctcaca acctgacagc agcagcctgt cggacttaac
      301 acgacgaatc ggcttgggtt agaatccggg gacgctgatt cgctttctcc gcttcccgga
      361 cttctcccgc atgaagaact tccgtgctgt cgaccgcgac actggcttcc tgatgccgcc
      421 atcggtggat gaatggctgc cggagaggca tctggcgcgg ttcgtggtgg aggtggttgc
      481 cagcctcgac ctgcgggcga tgaccggcag ctaccgcggt tccggtgagg cgtcgtatca
      541 cccggcggtg ctgctgggca tcctggtcta cggctacgcc accggtgtgt tctccagccg
      601 caagctggag cgggcgaccc atgattcggt cgcgttccgc ttcgttgcgg tcaatcagca
      661 cccggaccac gacaccattg cggcgttccg gcggcgcttc ctcaagccga tcgaggcctt
      721 gttcgtcgag gtattgcaac tcgcccgtga aatgggcgtg ctgaagctgg gcacgatcgc
      781 gctcgacggc accaagatcc atgccaacgc cagccgccac agcgcgctgt cgtatgagca
      841 tgcgggcaag atcgaggcgc agttgcaggc agaagttgcc gaactgatgg ccaaggctga
      901 agcggcggac caggccgacg tgccggacgg gatgtcgatc cctgaggagt tggcccggcg
      961 cgagcagcgt ctggcggaaa tcgctcgggc aaaggcgatc atcgaggcgc gggcaaagga
     1021 gcggcacggc cgcgagcagg ccgagtacga tgccacaatg gcagcgcgcg aagccaaggc
     1081 cgcagccacc ggcaagaagc ctggcggcag gccgcccacg ccgccggtgg ccgagcccgg
     1141 cccgaccgac caggtcaacc tgaccgacga ggagagccgc atcatgccgg tgccgggcgg
     1201 tggcttcgag cagtgctaca acgcgcaggc cgcggtggcg gcgggcagcc tgctggtggt
     1261 cgccactgac gtggtgcagg ccagcaacga caagcagcag gtcgtgccga tgctggcgaa
     1321 gctcgacaac ctgccggacg agttgggcgc aatcgaaacg ctgctggctg acacaggata
     1381 tttcagcgag gccaacgtgc aggcctgcgt ggcggcggag atcgatccgc tgctcgcgat
     1441 gggacgccag ccgcaccacc caccgctcgc cgagcgcttc gccccagccc cgccaccgcc
     1501 ggacaaccca acgccggttg aggcgatggc gtatcggctg aaaacgcctg agggccgggc
     1561 gctgtatgcc cggcgcaagc acacccccga gccggtgttc ggcatcatca aatcagcgct
     1621 cggcttccgc cagttcctgc tccgtggcct cgacaacgtc cgcggtgagt ggaacctggt
     1681 gaccatggcg tacaacgtga aacggctgtt cgccctcgca gccgctgcct gagccccgta
     1741 aggggctgtc acggcagaat tgatgcagtg gcagcacgat ctggaccccc gggacgtgaa
     1801 tcaggcgtcc caacttggca aaagcgagaa ggcgccctcc aaggcgtccc tacgctcgct
     1861 ggttccgcac agccaaaccc tccggctgac aactcaactc cgacaggctg ctagggcgct
     1921 gttttatcgc gccgtccgcc gattgacgtg gcacagagtg gtgcctgggg gtttttatag
     1981 atccggccct cattatcttg aataaacgct gagagccacc acgtcccaag atatgaagca
     2041 cgtggacatg cgcaagttgg ctccggcggc ccaggaggag agacggc
//
LOCUS       DATNBV010000428         2599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_552585,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000428 DATNBV010000000
VERSION     DATNBV010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552585"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..835)
                     /locus_tag="VN627_10170"
     CDS             complement(23..835)
                     /locus_tag="VN627_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03660.1"
                     /translation="MSGFLHQLAARSLGLAPAARPRAALPYAAPAPETPAPDLAAVAP
                     EAPAVVAAAPLPATWRDVSEPAAVLPAERHAAKATPASHAPAASTEIPARHSVALAPI
                     VPRQRPEINWEGDHPAIPEPAIGELPAPTAAIPNARPPAIQAAADSRPASDEPTTGLL
                     DLESLISRLVGQATSHAPLAPDGTPSPSTAIAPATGQPPRPGHTSHRPAERRESAETA
                     SPAADSAPEVHITIGRLEVNPPSRPTPPPPRPRGQAPLSLGDYLARRAGGRS"
     gene            complement(843..1541)
                     /locus_tag="VN627_10175"
     CDS             complement(843..1541)
                     /locus_tag="VN627_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03661.1"
                     /translation="MQALSTTQLLALWDAGQTRHPLDRALLALALAMPEQAPEQLADQ
                     PVGWREIHLLALRNAMFGNALDGYASCPSCASLMEFSLDSGALLAELPQPPSAARVVL
                     DGGQWRLPTSRDQAMILDAADPETAVAILLERCRIGQPVGNSPALIAELERRLEALDP
                     AANIRLGMQCTDCGQAWDAVLDIGSCFWAELGTCARRLLESVHRLAAAYGWREADILA
                     LSPARRAAYLSLIG"
     gene            complement(1554..2078)
                     /locus_tag="VN627_10180"
     CDS             complement(1554..2078)
                     /locus_tag="VN627_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HXD03662.1"
                     /translation="MAQFTVNAQRFDPYKNFKFRVKWDGRYVAGISKVSSLKRTTEVV
                     EHREGGDPSSARKSPGRSKFEAITLERGVTHDTNFEQWANKVWNLGSGLGSEVSLKDF
                     RKDLIIEVYNEAGQLALAYKVFRCWVSEYQALPDLDANANAVAIQHIKLENEGWERDY
                     EVGEPTEPSFVEPG"
     gene            complement(2126..>2599)
                     /locus_tag="VN627_10185"
     CDS             complement(2126..>2599)
                     /locus_tag="VN627_10185"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HXD03663.1"
                     /translation="AMAVPLTDGENGVLNPLGVNCLRTFPVYGAVSWGARTLRGADQM
                     SSEYKYVPVRRLALYIEESLYRGTQWVVFEPNDEPLWAQIRLNIGAFMNSLFRQGAFQ
                     GSSPREAYLVKCDRETTTQDDINRGVVNILVGFAPLKPAEFVVISIQQLAGQIQV"
BASE COUNT          356 a          796 c          997 g          450 t
ORIGIN      
        1 ccggcaatgg ccagggcgtt gctcacgagc ggcctccggc gcggcgggcg agatagtcgc
       61 ccagcgacag cggcgcctgg ccgcgcggac ggggcggtgg tggggtcggg cggctgggcg
      121 gattgacttc caggcggcca atggtgatgt gcacttcggg cgccgaatcg gccgccgggc
      181 tggcagtttc ggcgctttcc cggcgttcgg ccgggcggtg gctggtgtgg cccggccgag
      241 gtggctggcc ggtggccggc gctatggcgg tcgacgggga gggcgtgccg tccggcgcca
      301 gcggggcgtg gctggtcgct tggccgacga ggcggctgat cagcgattcc agatcgagca
      361 aaccggtggt cggctcgtcg ctggcgggcc ggctgtccgc tgccgcttgt attgccggcg
      421 ggcgggcgtt cgggatggcg gcggtggggg ccggcagctc gccgatggcg ggctcgggaa
      481 tggcaggatg gtcgccttcc cagtttattt ccgggcgttg gcgcgggacg attggcgcta
      541 gcgccacgct gtgccgggcc gggatttccg tactggccgc cggggcatgg ctagccggtg
      601 tggctttggc ggcatgccgt tcagcgggca gaacggcggc cggctcggat acgtcgcgcc
      661 aagtggcggg tagcggcgcg gcggctacga cagcgggcgc ttcgggcgcc accgcggcca
      721 aatccggggc tggcgtttcc ggcgctgggg cggcgtaagg cagggcggcc cggggccggg
      781 cggccggggc gaggcccagg ctgcgggcgg cgagttgatg gaggaatccg ctcatcgccg
      841 tcctagccga tcaggctcag gtaggccgcc cggcgcgccg gactcagggc caggatgtcg
      901 gcctcgcgcc agccgtaggc ggcggccagg cgatgcacgg attcgagcag gcggcgggcg
      961 caggtgccga gttcggccca gaagcagctg ccgatgtcga gcacggcatc ccaggcctgg
     1021 ccgcagtcgg tgcactgcat gccgagccgg atgttggcgg cggggtcgag cgcttcgagc
     1081 cgcctttcga gttcggcgat cagggccggc gagttgccga ccggctggcc gatccggcaa
     1141 cgctcgagca ggatggcgac ggcggtttcg gggtcggcgg cgtcgaggat catcgcctga
     1201 tcgcggctgg tcggcagccg ccattgcccg ccgtcgagca ccacccgcgc cgcgctcggc
     1261 ggctgcggca gttcggcgag cagggcgccg gagtcgaggc tgaactccat caggctggcg
     1321 caggacggac agcttgcgta gccgtccagc gcattgccga acatcgcatt gcgcagggcg
     1381 agcagatgga tctcgcgcca gccgaccggc tggtcggcca gttgttcggg cgcctgctcg
     1441 ggcatggcca gggcgagcgc cagcagggcg cggtcaagcg gatggcgcgt ctgtccggcg
     1501 tcccacaggg cgagaagttg ggtggtgctc agcgcttgca tggcgaggct ccatcagccc
     1561 ggctcgacaa agctcggttc ggtcggttcg ccgacttcat agtcgcgctc ccagccttcg
     1621 ttttcgagct tgatgtgctg gatggccacg gcgttggcat tggcatcgag gtcgggcagc
     1681 gcctggtatt cggaaaccca gcagcggaag accttgtagg ccagcgccag ttgtccggcc
     1741 tcgttataga cctcgatgat caggtccttg cggaagtctt tgagcgagac ttcgctgccc
     1801 aggccggagc cgaggttcca gaccttgttg gcccactgct cgaaattggt gtcgtgggtg
     1861 acgccgcgtt ccagcgtgat cgcctcgaac ttgctgcgcc cgggcgactt gcgggcgctc
     1921 gacgggtcgc cgccttcgcg gtgttcgacg acctcggtcg tgcgcttgag cgacgacacc
     1981 ttgctgatcc cggcgacgta acggccatcc catttgacgc ggaatttgaa attcttgtag
     2041 ggatcgaagc gttgggcatt gacggtgaac tgggccattt ttcagtctcc tcgaatatcg
     2101 gttcagtttg tcctggcgac gacggtcaga cctggatctg cccggccagc tgctggatgc
     2161 tgatgaccac gaattccgcc ggcttgagcg gcgcgaagcc gaccaggatg ttcacgacgc
     2221 cgcggttgat gtcgtcctgg gtggtcgttt cgcggtcgca cttgaccaag taggcctcgc
     2281 gcggcgaact gccctggaag gcgccctggc ggaacaggct gttcatgaag gcgccgatat
     2341 tcaggcggat ctgcgcccac agcggttcgt cgttgggttc gaagaccacc cactgcgtgc
     2401 cccggtagag cgattcctcg atgtagaggg cgaggcggcg cactggcaca tacttgtact
     2461 cggaactcat ctggtcggcg ccacgcaggg tacgggcgcc ccagctgacc gcgccataga
     2521 ccgggaaggt gcgcaggcaa ttgacgccga gcgggttgag cacgccgttt tcgccatcgg
     2581 tcagcggcac ggccatggc
//
LOCUS       DATNBV010000429         3128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1225988,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000429 DATNBV010000000
VERSION     DATNBV010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225988"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VN627_10190"
     CDS             complement(<1..292)
                     /locus_tag="VN627_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03664.1"
                     /translation="MTNAQHIKPPTQEQLDSATVKADQQFAMDTIIRGYSGTFANRFH
                     VQLEPGGTGAILGIGSTGILAGTTPDGEVFVNPVATVNQSFRVDQVFLRSLAE"
     gene            506..1894
                     /locus_tag="VN627_10195"
     CDS             506..1894
                     /locus_tag="VN627_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03665.1"
                     /translation="MRLSLRGPAALMLVAVLSLLLLIERPVWDVDVFWQLRLGELILG
                     ARGPPSAEPFAARHLGEHLPALAWAGQALYAGLREIGGWSLLRTVDALCWLGGFWAAA
                     LAARRRGAAALGLALALALGLIAALPQASVRPQSMGVLCFGLLIVLAFRPMRAGPRLV
                     LAALLLLVWQNLHPSVAIGAVWLGALAALRCAGWLRTREGPLPWEPVALAAMAAAATV
                     LTPEGPGIFAVSAANATASRAMGVSEWLPLWHPVNRQVAVPILAVALLSGWLVRRTGR
                     WAADEVVPALAMLVLTVVSYRFVLFWAVALVPVIARTAPPSAEARLPWYLRTVPLAVA
                     IGLGILLPARFDPSLPLGEVAQLRAASVKGTIYAHFPWGGPLIDAGYPAWRVAYDGRY
                     YRYTAAEWQAYGAASRGEMTLAAVEATWHPAAFLLDPDWNAPLVSALRADRARWRELP
                     GGGRAIAFIPAK"
     gene            complement(1946..2425)
                     /locus_tag="VN627_10200"
     CDS             complement(1946..2425)
                     /locus_tag="VN627_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXD03666.1"
                     /translation="MTIAVGDKLPDVKLIKATAEGSEPVQTAEYFKGKKVALFSVPGA
                     FTPTCSAKHLPGFVEKAAELKAKGIDEIACTAVNDAFVMKAWGAANGSDDVTMLADGN
                     GDLVKALGLVMDGSGFGMGQRGQRFSMIVNDGTVEQLNVEGPGEFKVSAADYMLGQL"
     gene            2656..3120
                     /locus_tag="VN627_10205"
     CDS             2656..3120
                     /locus_tag="VN627_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-L-homocysteine hydrolase"
                     /protein_id="HXD03667.1"
                     /translation="MSNRWKGAAMAAAALATGMAVPAQAAARGEAEQLRRLDIMLMVT
                     GLRCRTTADDFRADFAAFEKAHLPTLNAAAAELRDGLAGSAGPAGAARALDRISTGMA
                     NQYGQGHPWLDCAQLKQVTRVLTKVQGRATLVEAADELLAPSEGSKFAYARR"
BASE COUNT          434 a         1004 c         1121 g          569 t
ORIGIN      
        1 tctccgcgag agaacggaga aacacctgat ccacgcgaaa cgactgattc acagttgcca
       61 cagggttgac gaaaacttcc ccatcaggtg ttgtgccagc cagtataccg gttgaaccta
      121 ttccgagaat agcgccggtc ccgcccggct ctaattgaac gtggaaccgg tttgcaaatg
      181 tgccagagta tcccctaatt atcgtgtcca tcgcgaactg ctggtcagcc ttaactgtcg
      241 ccgagtccaa ctgctcttga gttggcggtt taatatgttg tgcgttggtc atatcccatc
      301 atatttctga cctcgaattg gagtcaattt cgtatccgat cgggggtggg tccaaattag
      361 ttcaaggcat ccatctcggg gtggtcccct tcaggatcaa aggtcctgat ggcctgtcca
      421 ggactgagtg accggccttg cgcctttccg cttcacgcgg cactttgcgg gcgtggggga
      481 accagcacaa actgacgagg ccgtagtgcg gctttcgctc cgagggccgg cggcgcttat
      541 gctggttgcc gtgctgtcgc tgctgctgct catcgagcgg ccggtgtggg atgtcgacgt
      601 gttctggcag ctgcggctgg gggaactgat ccttggcgcg cgcggtccgc cttccgccga
      661 gccgtttgcc gcccggcatc tgggagaaca cctgccggcg ctggcctggg cggggcaggc
      721 tctttatgcg ggcctgcgcg agatcggcgg atggtcgctg ctgcggacgg tcgacgcgct
      781 gtgctggctg ggcgggttct gggcggcggc cttggcggcg cggcggcgcg gggctgcggc
      841 cctggggctg gcgcttgcgc tggcgctcgg gctgattgcg gccctgccgc aggcctcggt
      901 tcgtccgcag agcatgggcg tgctgtgctt cgggctgctg atcgtgctgg ccttccgccc
      961 gatgcgggca gggccgcggc tggtgctggc ggcgctgctg ctgctcgtct ggcagaacct
     1021 gcatccctcg gtggcgattg gcgcggtctg gctgggggcg ctggccgcgc tgcggtgcgc
     1081 cgggtggctg cggacgcggg aaggcccgct gccgtgggag ccggtggcgc tggcagccat
     1141 ggcggccgcg gcgacggtgc ttacgcccga ggggcctggc atcttcgccg tctccgccgc
     1201 caatgccacg gccagccgtg ccatgggggt gagcgagtgg ttgccgctgt ggcacccggt
     1261 caaccggcag gttgccgtgc cgatcctggc ggtggcgctg ctgtccggtt ggctggtgcg
     1321 gcggaccggg cgctgggcgg cggacgaggt ggtgccggcc ctggccatgc tggtgctgac
     1381 cgtggtgagc taccgctttg tgctgttctg ggccgtggcg ctggtcccgg tcatcgcccg
     1441 caccgcgccg cccagcgccg aggcccgctt gccgtggtat ctgcggaccg tgccgctggc
     1501 cgtggcgatc ggccttggca tcctgctgcc ggcgcggttc gacccctcgc tcccgctggg
     1561 cgaggttgcg cagctgcggg cggcgagcgt caagggaacg atctacgccc atttcccctg
     1621 gggcggtccg ctgatcgacg cgggctatcc ggcctggcgg gtggcttatg acgggcgcta
     1681 ctatcgctat accgccgccg aatggcaggc ctacggggcg gcgagccggg gcgagatgac
     1741 gctcgcggcc gtggaggcga cctggcatcc ggccgccttc ctgctcgatc cggactggaa
     1801 cgccccgctt gtctcggccc tcagggcgga ccgggcgcgc tggcgcgagt tgccgggcgg
     1861 gggcagggcc atcgccttta ttccggccaa atgataaggg cgccccttgc gggacgccct
     1921 tatcgcggaa gcgcgggaga tcggctcaga gctggccgag catgtagtcc gccgcgctga
     1981 ccttgaattc gccggggcct tcgacgttca gctgctcgac ggtgccgtcg ttgacgatca
     2041 tcgagaagcg ctggccacgc tggcccatgc cgaagccgct gccgtccatg acgaggccga
     2101 gggccttgac caggtcgcca ttgccgtcgg ccagcatggt cacgtcgtcc gagccgttgg
     2161 ccgcgcccca ggccttcatc acgaaggcat cgttgacggc ggtgcaggcg atctcgtcaa
     2221 tccccttggc cttgagttca gcggcctttt ccacgaagcc gggcaggtgc ttggccgagc
     2281 aggtcggcgt gaaggcgccg gggaccgaga acagggcgac cttcttgccc ttgaagtatt
     2341 cggccgtctg caccggctca ctgccttcgg cagtggcctt gatcagcttc acgtcgggca
     2401 gcttgtcgcc aacagcaatc gtcatggtct tgcactcctt catcggggtg gggtgatgcc
     2461 ctgccatagg gcgcattgcg agtgcttcgc aaccgccgat cacacgagcc ggtccggccc
     2521 tgccaccaaa ggtaaaatgc gacgagccga gcatggtaac catggggtta agcgcgaagc
     2581 gtgacccgca attaagcctg tgcgccgcaa tttctgccgc ggggaaacaa ggatcttttt
     2641 gcggagacca ggcagatgag caacaggtgg aaaggcgcgg cgatggccgc agcggcgctg
     2701 gcgacgggca tggccgtgcc ggcccaggcg gcagcccggg gcgaggcgga gcagctgcga
     2761 cgcctcgata tcatgctgat ggtgacggga ctgcgctgcc gcaccacggc cgatgacttc
     2821 cgggccgact tcgcggcctt cgagaaggcg cacctgccga cgctcaatgc cgccgccgcc
     2881 gagctgcgcg acggtctggc cggcagcgcc ggcccggcgg gggccgcccg cgcgctcgac
     2941 cggatcagca ccggcatggc caaccagtat ggccagggtc acccctggct cgactgcgcc
     3001 cagctcaagc aggtgacccg cgtcctgacc aaggtgcagg gccgcgccac gctggtcgag
     3061 gccgccgacg aactgctggc accgagcgag ggcagcaagt tcgcctatgc ccggcgctga
     3121 ccggcaca
//
LOCUS       DATNBV010000430         4144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2166246,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000430 DATNBV010000000
VERSION     DATNBV010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166246"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1023)
                     /locus_tag="VN627_10210"
     CDS             complement(61..1023)
                     /locus_tag="VN627_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HXD03668.1"
                     /translation="MTIAAHGGPEVLQWAPVDLPPPGPGEVRLRTRAAGLNFIDTYHR
                     SGLYPQPLPTGLGLEGAGVVEALGEGVTGWQPGDRAGIFTGPPGAYATARNVPAAQLV
                     RLPDSVPDEVAAAIMLKGATAEFLVERCARVQPGSPVLVHAAAGGVGQLLVQWLKHIG
                     AAVIGTAGGAAKVALAGAAGADHVIDYAVEDVAARVRDLIGGEGAQVVFDGVGAATWE
                     ASLAASARRGLIVSYGNASGPVTGVALASLAAAGSLYVTRPTLFDYYRAPEERAAGMA
                     RLFGLVAAGVLSVKIGQRFALKDAAQAHRALEARQTTGSTVLLP"
     gene            1250..1882
                     /locus_tag="VN627_10215"
     CDS             1250..1882
                     /locus_tag="VN627_10215"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXD03669.1"
                     /translation="MEQFAWGRRTPEVAEDGDHGDWGEERRGSSRVTVMIRPAKLIAD
                     KREFLCVVRDVSENGLKLRLFHALPEHGHLTIEFENGDRHAIQLVWQNGELAGCLFVD
                     EVDLPRLIAAHQGPYPRRQPRLHVAVEAVLCTGGLRTPITMRDLSQRGAAIDCPGWLM
                     IDELVRIESKVLPTIHAKIRWRRPPRYGVVFEQTFRLEELALACARLGQE"
     gene            complement(1892..3004)
                     /locus_tag="VN627_10220"
     CDS             complement(1892..3004)
                     /locus_tag="VN627_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HXD03670.1"
                     /translation="MAFIPADQRELFLPLIEGISEDPPWGTFMRNLAARTYARRAFLI
                     ITLANAMPTQEPTILHFAAPRAAHEPPLDFERIAGLRLHPYGQLRPERVYSLEEMLDF
                     SDLEHLAAQRAALDEMGIRFGRWLRVSAGTADAWLVLVREREDFTAAAVATLSAIAPL
                     FAVALRAQVERAEQVLQTAMAHHALGGIGVGQLAFDREGRIMAADPVAQGALPIAEDP
                     GGRPGRRLQVLPDIARQVDEACAAIADGAPGAVSVIALDRHRGLFLLLRQADLDLPMP
                     SAVPALIGTVRIAPPANARSIIAAIASLHGLSDREAALAYGLVRGESIVEAGTRLRLT
                     PETARNYSKRIYAKTRTSGQADLVRLILTGLAPFAG"
     gene            3092..4012
                     /gene="cysD"
                     /locus_tag="VN627_10225"
     CDS             3092..4012
                     /gene="cysD"
                     /locus_tag="VN627_10225"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HXD03671.1"
                     /translation="MSDHNAKSALTHLERLEAEAIHIIREVVAEAERPVMLYSVGKDS
                     AVMLHLARKAFYPSPPPFPLLHVDTTWKFQEMYKLRDKMAELSGMELLVYHNPEAMER
                     GINPFDHGALHTDMWKTEGLKQALDKWQFDAAFGGARRDEEKSRAKERIFSFRTASHG
                     WDPKNQRPELWNLYNARKNKGESIRVFPISNWTELDVWQYIHLNDIPIVPLYFAAKRP
                     TVERDGMLLMVDDDRFPLKPGEVPVERSIRFRTLGCYPLTGAVESEAKTLPEVIQETL
                     LTTTSERQGRAIDKDAGGAGMEVKKQQGYF"
BASE COUNT          731 a         1294 c         1498 g          621 t
ORIGIN      
        1 attgccacaa aaggtattga tatttaatat aattaaccgg agtttccggg gggcttccga
       61 tcatggcagg aggacggtgg agccggtggt ctggcgggct tcgagggcgc ggtgggcttg
      121 ggcggcgtcc ttcagggcga agcgctggcc gatcttgacc gagaggaccc cggcggcgac
      181 gaggccgaac aggcgagcca tgccggcggc gcgttcctcg ggtgcgcggt aatagtcgaa
      241 cagggtcggg cgggtgacgt agagcgagcc ggcggcagcg aggctggcga gggcgacgcc
      301 ggtgaccggg ccgctggcgt tgccgtagct gacgatgagc ccgcgccgcg cgctggcggc
      361 gagggaagcc tcccaggtgg cggcgccgac gccgtcgaac acgacttgcg cgccttcgcc
      421 cccgatcagg tcccgcaccc gggcggcgac gtcttccacg gcgtagtcga tcacgtggtc
      481 cgccccggcg gccccggcca gggcaacctt ggcggcgccg ccagccgtgc cgatgaccgc
      541 ggcgccgatg tgcttcagcc attggacgag caattggcca accccgcccg cggcggcatg
      601 aaccagcacc ggcgagccgg gctggaccct ggcgcagcgt tcgacgagga attcggccgt
      661 ggcgcccttg agcatgatgg cggcggcgac ttcgtccggc acggaatccg gcaggcgcac
      721 gagctgggca gcgggcacgt tgcgcgccgt ggcataggcg ccgggcgggc cggtgaagat
      781 cccggcgcga tcgcctggct gccaaccggt gacgccttcg cccagggctt cgaccacgcc
      841 ggcgccttcg aggccgaggc cggtgggcag gggctgcgga tagaggccgg agcgatggta
      901 ggtgtcgatg aaattgagcc cggcagcccg ggtgcgcagg cggacctcgc cggggcccgg
      961 cgggggcaga tcgacgggcg cccactgcag gacttcgggc ccgccgtgcg ccgcgatggt
     1021 catgcggtgc tcgggacgct gttcagtggc tgcgcaaggc tcggtcatgc gcgtgggttt
     1081 ggcagcttgg acagcaaggt tcaaccgcgg gagaagtgca gtcgccccgt cagatatcgt
     1141 ttgcatgctt atgaaagacc gagatttttt aaggcgacgg taagggtttt ggcagatgat
     1201 cgggaaaacg ccgtcgggaa acggcgccgg gaagccgagg gtttgcatca tggaacagtt
     1261 cgcatgggga aggcggacgc cggaggttgc cgaggacggt gatcacggcg actggggcga
     1321 ggaacggcgc gggtcgtcgc gggtcacggt catgatccgg cccgccaagc tgattgccga
     1381 caagcgtgaa tttctctgcg tggtgcgcga cgtatccgag aacgggctca agctgcggct
     1441 gtttcacgcc ctgcccgagc acggccacct gaccatcgag ttcgagaatg gcgaccgcca
     1501 tgcgatccag ctggtgtggc agaacggcga actggcggga tgcttattcg tcgacgaggt
     1561 tgacctgccg cgcctgattg ccgcgcatca aggcccctat ccacgccgcc agccacggct
     1621 gcacgtcgcc gtggaagccg tgctgtgcac cggcggcctg cgcacgccaa tcaccatgcg
     1681 cgatctatca cagcgcggcg cggcgatcga ttgtccgggc tggctgatga tcgacgagct
     1741 ggtgcggatc gagagcaaag tgctgccgac gatccacgcc aagattcgct ggcgccgccc
     1801 cccgcgttat ggcgtggtgt tcgagcagac cttccggctg gaagaactcg cgctggcctg
     1861 cgcgaggctg gggcaggagt gataccaggt ttcacccagc aaaaggggcg agaccggtaa
     1921 ggatcaggcg gaccaggtcg gcctggccgc tggtgcgggt cttggcatag atgcgcttgg
     1981 aatagttgcg ggcggtttcc ggggtgaggc gcaggcgcgt tccggcctcg acaatggatt
     2041 cgccgcgcac cagcccatag gccagcgccg cttcacggtc cgagaggccg tgaagcgatg
     2101 ctatggccgc gatgatgctg cgggcgttgg ccggaggggc aatgcggacc gttccgatca
     2161 gggccggcac ggcgctgggc atcggcaggt ccagatccgc ctggcggagg agcaggaaca
     2221 ggccgcgatg ccgatcgagc gcgatgacgc tgacggcgcc cggcgccccg tcggcgatgg
     2281 cggcgcaggc ctcgtccacc tgccgggcaa tgtcggggag gacttgcagg cggcgccccg
     2341 gccggccccc ggggtcttcg gcaatcggca gggcgccttg cgccacggga tcggcggcca
     2401 tgatccgccc ttcgcgatcg aaggcgagct gccccacgcc gatcccgccc agcgcgtggt
     2461 gcgccatcgc ggtttgcagc acctgctcgg cccgttccac ctgggcacgc agggccacgg
     2521 caaacagcgg ggcaatggcg ctgagcgtgg ccacggccgc tgcggtgaaa tcctcacgct
     2581 cgcggaccag caccagccag gcgtcggccg tgccggcgga gacgcgcagc caccgcccga
     2641 agcgaatgcc catctcgtcc agcgcggcgc gctgcgcggc gaggtgttcg aggtcgctga
     2701 aatccagcat ctcctcaagg ctgtaaaccc gctccggccg taactggcca tagggatgaa
     2761 ggcgcaggcc ggcgatccgc tcaaagtcga gcggcggctc gtgggcggcg cggggggcgg
     2821 cgaaatgcag gatcgttggc tcttgcgtgg gcatggcatt ggccagcgtg atgatcagga
     2881 acgcgcggcg ggcgtaggtc cgcgcggcga ggttgcgcat gaaggtgccc cacggcgggt
     2941 cctcggagat gccctcgatc agggggagga acagctcgcg ttgatcggca ggaatgaagg
     3001 ccatgcattt tgctcccata tgggagcttc ggggtcaagg cgagtcagcg atgagcgggg
     3061 caacgacaga accgagagag ggacaaagtc gatgtccgac cacaatgcga agtccgcgct
     3121 gacccacctc gagcgacttg aggcagaggc cattcacatc atccgcgaag tcgtcgccga
     3181 ggcggagcgc ccggtgatgc tctactcggt gggcaaggac agcgcggtga tgctgcacct
     3241 ggcgcgcaag gcgttctacc cgagcccgcc gccgttcccg ctgctgcacg tggacacgac
     3301 ctggaagttc caggaaatgt acaagctgcg cgacaagatg gctgagcttt cgggcatgga
     3361 gctgctggtc taccacaatc ccgaggcgat ggagcggggg atcaatccgt tcgaccacgg
     3421 cgcgcttcac accgacatgt ggaagaccga agggctcaag caggctctcg acaagtggca
     3481 gttcgatgcg gccttcggcg gcgcgcgccg cgacgaggaa aagagccgcg ccaaggagcg
     3541 catcttctcg ttccgcacgg ccagccacgg ctgggacccc aagaaccagc ggcccgagct
     3601 gtggaacctc tacaacgccc gcaagaacaa gggcgaatcg atccgcgtct tcccgatctc
     3661 gaactggacc gagctggacg tgtggcagta tatccacctc aacgacattc cgatcgtgcc
     3721 gctgtacttc gcggccaagc gcccgactgt ggagcgcgac gggatgctgc tgatggtcga
     3781 cgacgaccgc ttcccgctga agcccggcga agtgcccgtc gagcgttcga tccggttccg
     3841 cacgctgggt tgctacccgc tgaccggcgc cgttgagagc gaggccaaga ccctgccgga
     3901 ggtgatccag gaaacgctcc tgacgacaac ctcggagcgc cagggccgcg ccatcgacaa
     3961 ggatgccggc ggcgccggca tggaagtgaa gaagcagcag ggctatttct aggttgcacc
     4021 gatattcggc ccataccggc cacccagtgc ctagtccgca ccgccatcgc caaacgcgcg
     4081 atggatgctg aagcaagttc agcatgacgg agagtaagat gtctgaccag accaccacca
     4141 ccga
//
LOCUS       DATNBV010000431         3418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3066146,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000431 DATNBV010000000
VERSION     DATNBV010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3066146"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /gene="sdhA"
                     /locus_tag="VN627_10230"
     CDS             <1..1566
                     /gene="sdhA"
                     /locus_tag="VN627_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HXD03672.1"
                     /translation="DWLGDQDAIEYMVREAPAAVYELEHAGVPFSRNADGTIYQRPFG
                     GHMQNMGEGPPVQRTCAAADRTGHAMLHALYQQSLKYDADFFIEYFAIDLIMENGACR
                     GVIAICMDDGSIHRFKSHSVVLATGGYGRCYYTATSAHTCTGDGGGMVLRAGLPLQDM
                     EFVQFHPTGIYGAGVLITEGARGEGGYLTNSEGERFMERYAPSAKDLASRDVVSRSMA
                     LEIREGRGVGPHKDHIYLHLDHIDPNVLAERLPGITESGKIFAGVDLTRQPLPVVPTV
                     HYNMGGIPTNYHGEVVTIGADGNPETVVPGLFAVGEAACVSVHGANRLGSNSLIDLVV
                     FGRATGHRLKELIKPGTAHAELPKDSADLALTRLDHFRNAAGSTPTAEIRAEMQRTMS
                     LHAAVFRTDELMAEGKDKLAATYQKMNDIKVTDRSLIWNSDLIETLELDNLISQASVT
                     LHGAHNRKESRGAHAHEDFPDRDDAGWMKHTAAWFEGWGGKGGSVRIDYRPVHEFTLT
                     DDVEYIKPKKRVY"
     gene            complement(1700..2230)
                     /locus_tag="VN627_10235"
     CDS             complement(1700..2230)
                     /locus_tag="VN627_10235"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXD03673.1"
                     /translation="MPASAAAVMGTTDCTLSDVSPGATDCQGWFQGNLNSGNATDLAN
                     EALIINDLLGTSYTGSNLPFVDVTNLSGSTITFAQALTGTVILGVHVGAANGAGGIGY
                     NGTAFYTLVDPGSSVTLNIPGLSNARVFGVAAVPEPGTWAMMLLGLGSLAWLIRRNRR
                     AVRPGTTTITYKGFAT"
     gene            complement(2476..3228)
                     /locus_tag="VN627_10240"
     CDS             complement(2476..3228)
                     /locus_tag="VN627_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXD03674.1"
                     /translation="MRGFPVGERTGILAPAEPTFTRLPALATWNEEEDMANRQLTPYR
                     RGGLGSGFGAGFGGGFGGSSLFDLHRQMNRLFDDLVERGSGSGAGGQGMSLAAPALEL
                     HQSDDKLEITAELPGVRQEDIDLHVEDGVLTLSGEKRSERSDEQRGYSERSYGRFERS
                     IALPANVDEEACKAEFKDGVLTITLPRAAEKQRGRKIPLGGSSSGARQQPQSGQQAHN
                     DTHEPPRQQAAEQPTGSSGQQGRETKGSGQQG"
BASE COUNT          580 a         1120 c         1151 g          567 t
ORIGIN      
        1 gactggctcg gcgaccagga cgcgatcgaa tacatggtgc gcgaggcccc tgccgcggtc
       61 tacgagctgg agcacgccgg tgtgcccttc agccgcaatg ccgacggcac gatctaccag
      121 cgcccgtttg gcggccacat gcagaacatg ggcgagggcc cgccggtcca gcgcacctgc
      181 gccgcggccg accgtaccgg ccacgccatg cttcacgcgc tctaccagca gagcctgaag
      241 tacgatgcgg acttcttcat cgaatacttc gccatcgacc tgatcatgga aaacggcgcc
      301 tgccgcggcg tgatcgcgat ctgcatggac gatggctcga tccaccgctt caagtcgcac
      361 tcggtcgtgc tggcgaccgg cggctatggc cgctgctact acaccgcgac ctcggcccac
      421 acttgcaccg gtgacggcgg cggcatggtg ctgcgcgcgg ggctgccgct gcaggacatg
      481 gaattcgtcc agttccaccc gaccggcatc tacggcgcgg gcgtgctgat caccgagggt
      541 gcgcgcggcg agggcggcta cctcaccaac tccgagggcg agcgcttcat ggagcgctac
      601 gcgccgagcg ccaaggacct cgccagccgc gacgtcgtca gccgctcgat ggcgctggag
      661 atccgcgagg ggcgcggcgt ggggccgcac aaggaccaca tctacctgca cctcgaccat
      721 atcgatccga acgtgctggc cgagcgcctg ccggggatta ccgagagcgg caagatcttc
      781 gccggcgtgg acctcacccg ccagccgctg ccggtggtgc cgaccgtgca ctacaacatg
      841 ggcggcatcc ccacgaacta ccacggcgag gtggtcacca tcggcgccga cgggaacccg
      901 gaaacggtcg tccccggcct gttcgcggtg ggcgaggcgg cctgcgtctc ggtccatggc
      961 gccaaccgcc tcgggtccaa ctcgctgatc gacctcgtgg tcttcggccg cgccaccggc
     1021 catcgcctca aggagctgat caagccgggc accgcgcatg ccgaactgcc caaggattcg
     1081 gccgacttgg cgctgacccg gctcgaccac ttccgcaatg ccgccggttc cacccccacg
     1141 gccgaaatcc gcgccgagat gcagcgcacg atgagccttc acgccgccgt gttccgcacc
     1201 gacgagctga tggccgaggg caaggacaag ctcgcggcca cctaccagaa gatgaacgac
     1261 atcaaggtga ccgaccggtc gctgatctgg aactcggacc tgatcgagac gctggagctg
     1321 gacaacctga tcagtcaggc ttcggtcacc ctgcacggcg cgcacaaccg caaggagagc
     1381 cgcggcgccc atgcgcacga ggacttcccc gaccgtgacg atgcgggctg gatgaagcat
     1441 accgccgcct ggtttgaagg ctggggcggc aagggaggct ccgtgaggat cgactaccgt
     1501 ccggtgcacg aattcacgct cacggacgat gtggaataca tcaagccgaa aaagcgggtt
     1561 tactgatcgg cgctaaggcg ttcggaacac agaaaaggcc ggggcgggta cgctccggcc
     1621 ttttctgtgt tcgcacgtgt cgaccgcgtc gctttcccat ttgtcctgcg gaaaacgggg
     1681 agggacgtcg cggcccggat caggtggcaa agcccttgta ggtgatggta gtcgtgcccg
     1741 ggcgcaccgc gcggcggtta cggcggatca gccaggcaag gctgccgagg ccgagcagca
     1801 tcatggccca ggtgccgggt tccggaaccg cagcgacgcc gaatacgcgg gcgttcgaca
     1861 ggccggggat attgagcgtt acgctgctgc cgggatcgac cagcgtatag aaggcggtgc
     1921 cgttgtagcc gatgccgccc gcgccgttgg ctgccccaac gtgaacgccg aggatgaccg
     1981 tgccggtcag cgcctgcgcg aaggtgatgg tgctgccgga caggttggtg acgtcaacga
     2041 acggcaggtt gctgccggta tagctggtgc cgagcagatc gttgatgatc agcgcctcgt
     2101 tggcgagatc ggtggcgtta ccggagttca ggttgccctg aaaccagccc tggcagtcag
     2161 ttgccccagg tgagacatcg ctcaacgtgc agtcagtcgt gcccatcacg gccgctgcgg
     2221 aggcgggcat tgcgctgaac gcaagcgcgc agctgctggc aacggccagc aaggtcagac
     2281 gattagcagg acgcattatc gttactccac cactgacaac ggacgttgtc gaatgggtta
     2341 atgcaaagtt aacgaaaccg gttccccatt ttgctctcca ggtgcagatt ttctttcagg
     2401 gacgggcact tgtgccggtc tcctccccat tataccggct ccagcgcgga acatggggag
     2461 gatcgccgag cgggctcagc cttgctggcc tgagcccttg gtttcgcggc cctgctgacc
     2521 cgaactgccc gtgggctgct cggcggcttg ttggcggggt ggttcatggg tgtcgttatg
     2581 ggcctgctgg ccggactgcg gctgctgcct ggcgcctgag ctcgagccac ccagaggaat
     2641 cttgcggccc cgctgctttt cagccgcgcg cggcagggtg atcgtcagta cgccgtcctt
     2701 gaattcggcc ttgcaggctt cctcgtcgac attggcaggc agggcgatcg agcgctcgaa
     2761 acgcccatag ctgcgttcgg agtagccgcg ctgttcatcg ctgcgctcgg accgcttctc
     2821 gccgctgagc gtgagcacgc cgtcttccac gtggagatcg atatcctcct gccgcacgcc
     2881 gggcagttcg gccgtgattt cgagcttgtc gtctgactgg tggagttcga gcgccggggc
     2941 ggcgaggctc atgccctggc cacccgcgcc ggagccggag ccccgttcga ccaggtcgtc
     3001 gaacaggcgg ttcatctggc ggtgcaagtc aaacagcgag cttccgccga aaccgccgcc
     3061 gaagcccgcg ccaaagccgc tgccaaggcc gccgcggcgg tagggggtga gttgtcgatt
     3121 ggccatgtct tcctcctcat tccaggtggc gagggcgggc aggcgggtaa acgtcggttc
     3181 agcaggcgcg aggatgccgg tccgctcccc gactgggaaa ccccgcatgg ctggcgccgt
     3241 tccgcagcgt gtccgcccgc aggcgcggct ggtcgctcag cccaaggggg agtcgacgcg
     3301 gcggccagcc ttgctatcct gtggccgggc tggaagcgcg cacggcgaca gttacggctg
     3361 ccgatgccaa cggcgggcgg ctcgatgtca gcgtgaccgc gatccagacg gcaccggg
//
LOCUS       DATNBV010000432         6172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3066390,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000432 DATNBV010000000
VERSION     DATNBV010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3066390"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VN627_10245"
     CDS             complement(<1..763)
                     /locus_tag="VN627_10245"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03675.1"
                     /translation="MDTESAPGRMRVGFIGLGDQGAPMAEMIARAGFPLTVWARRAEV
                     IVRFGGLGARIAADPSDLAAECDAIGLCVTDDGAVRAVVYGGGLLAAMRAGSVLMIHS
                     TIDPALCREIARDADARGVMVLDAPVSGSAARALARTLLVIAGGEEAVLEQVRPVLES
                     YGNPIMHVGGVGAGMTAKLINNLMAAVNYATAYDALVVARDNGIDTDLLCALAASGSG
                     RNYGFELMPRAHRADRTLHIAAILGKDVELAIATVP"
     gene            934..1434
                     /locus_tag="VN627_10250"
     CDS             934..1434
                     /locus_tag="VN627_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD03676.1"
                     /translation="MNDLERLVAIEAIKDLFAKKLRYMDLKMWDGYASLHAEDAYSET
                     WKHSLPEDDQPVSDAGQKGRVVGPQAIAGQLARVFTHPVPITSVHHCHAAEIEFLSDT
                     EAKGIWSMEDSLWWQNGAVEEHLHGYGHYYETFRKVDGRWLFTSRQLIRLRVDMTPDF
                     WSRLNM"
     gene            complement(1462..3360)
                     /locus_tag="VN627_10255"
     CDS             complement(1462..3360)
                     /locus_tag="VN627_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HXD03677.1"
                     /translation="MSESASEPATGHDHHDSLATLAIGAIGVVFGDIGTSPLYALKES
                     FIGHHPLTVDPAHIFGVLSLIFWTMMLIVTIKYVFVILHADNHGEGGSLALLALIGRK
                     LGITRWTPIIGMLGVIATALFYGDAIITPAISVLSAVEGLTVVNDSFGYWVLPLSIGI
                     LVGLFAIQSHGTAVVGRLFGPVMIVYFGVLAVLGLVNIAHAPQVLGALSPRYALDFFL
                     IDPKLAFLALGSVVLAVTGAEALYADMGHFGRKAIMVSWLYIAMPCLLLNYLGQSALL
                     LKNPAAVENPFFLMAPEWSRLPLVILATLATIIASQAVISGAYSVSQQAVQLGYLPRL
                     RIAHTSARAAGQIYVPLVNWGLLILVILLVLGFRSSSNLAAAYGIAVTGTMFITACML
                     GVLTFAVWKWHPLLAAVVTGAFLAVDGVYFASNVTKIPDGGWFPLLVAAVAFTLLTTW
                     ASGRRLMRQRLAEDAMPVDVFLQSVADRVRRVAGSSVFLAASVEGIPHALLHNLKHNH
                     ILHQRVAILTVATKGVPHVAPGDRRMVEDLGHGFYRIVLNYGFMDEVDIPAELQAEDR
                     AGGPFKPAETSYFLARETLISSSRPGGMAVWREKLFAWMVRNAESAMQFFKLPTNRVI
                     ELGSQVEI"
     gene            3497..3793
                     /locus_tag="VN627_10260"
     CDS             3497..3793
                     /locus_tag="VN627_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03678.1"
                     /translation="MLSIVKRISGGRGRLPWELALRLGGVGVIAGAWLVARSLCRWSA
                     MPRPHPATFLEFAMTTAAFALLTTGLAFLVEGPGLFRQVPIPRHSAFFPASPAH"
     gene            3844..5379
                     /locus_tag="VN627_10265"
     CDS             3844..5379
                     /locus_tag="VN627_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HXD03679.1"
                     /translation="MDRDTRQNRPPKAGGAWGSPGGYVAALALVVIVTLAGLAIVSLG
                     NIANIGLLFLLPVMVAATRYGLRTGIVTSLASSLAYNFFFIPPTHTFTIENPQNIITV
                     MVLLGVAVAASQLAAQVRQQALLAQASAARNQALAGFARQLTGIGTAEALAEVCCTQA
                     SRLLEVNAVLLMPSGQGLEVRSAAPRPAELEFLDHAAARACFDTAQPAGRSTDSLTAS
                     EWQFHPITGAGHVLAVIGLSRADAGDPVAADQLPLLLGMIDQAGLALGRIALTDDMAA
                     IAQVRERDRLRHALLSSVSHDLRTPLTTIIGTLNQLRPADAEQAGQLAEMRVEAERLH
                     RFVANLLDMVRIEAGALRLAVEPVDLSDAVASALQDMDRALAGQPVNVAIPPELPLVQ
                     VDPQLFHHCLINLIDNAAKHGMSSGPIAISARREEHGVVLSVADEGPGIPAGQEQRIF
                     ETFTRLEGSDRKGGTGLGLAIVKGFCEAMGLAVAAANRADRPGACFSIHFPATRLKEL
                     PGA"
     gene            5376..6065
                     /locus_tag="VN627_10270"
     CDS             5376..6065
                     /locus_tag="VN627_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXD03680.1"
                     /translation="MTIPVTVLAVDDEAAIRRLLHASLDRAGYRVVEAGTARETIAAL
                     QIDKPDLVLLDLGLPDRDGLELIPLIKQAGAAIIVISARDATQQKVAALDLGADDYVS
                     KPFDSEEVLARIRVALRHRLAGETDVPRLRRGPVEIDLAARMVRRDGEEVHLTPREYG
                     FLAELAKAPGRVVTHAQLLRAVWGPGHETDVEYLRVAARSIRRKLEADPSRPRLFRNE
                     PGVGYRLMGEE"
BASE COUNT         1067 a         2164 c         1970 g          971 t
ORIGIN      
        1 ccggcaccgt ggcgatggcg agctcgacgt ccttgcccag aatggccgcg atgtgcagcg
       61 tccgatcggc gcggtgcgcg cgcggcatca gctcgaagcc atagttgcgc ccgctgccgg
      121 aggcagccag cgcgcacaac aggtcggtgt cgatgccgtt gtcgcgcgca accaccaagg
      181 catcgtaggc cgtggcatag ttgaccgccg ccatcaggtt gttgatcagc ttggccgtca
      241 tccccgcgcc caccccgcca acgtgcatga ttggattgcc atagctttcc agcaccgggc
      301 gcacctgttc gagcaccgcc tcctcgccac cggcaatcac cagcagggtc cgcgccaatg
      361 cccgcgcggc agaaccggag accggcgcgt cgagcaccat caccccgcgc gcatccgcat
      421 cgcgcgcgat ttcgcggcac agcgccggat cgatggtgga atggatcatg aggaccgagc
      481 cggcgcgcat cgccgccagc aagccgccgc catagaccac ggcgcgcacg gcgccgtcgt
      541 cggtcacgca caggccgatg gcatcgcatt cggccgcgag atcggagggg tcggcggcga
      601 tccgcgcgcc gagtccgccg aaccgcacga tcacctcggc ccgccgcgcc cagacggtca
      661 ggggaaaacc ggcgcgggca atcatctcgg ccatcggcgc cccctggtcg ccaagtccaa
      721 tgaagccgac gcgcatcctg cccggtgccg attccgtgtc cattctcgct ctcccgcccg
      781 agccatgcca ttcggacttg catcgcgcct gtgcaacggg gctcattacc gaaactatcg
      841 cccctctgtt gtaatcccta gtgccttgcg gcttggcggg acgcccggca cacacttgga
      901 tgaacggcca ggcaaaccgg gagcaggcag cagatgaacg atcttgaacg gctggtggcg
      961 atcgaggcaa tcaaggacct cttcgcgaag aagctgcgct acatggatct caagatgtgg
     1021 gacggctatg cttcgctgca cgccgaagac gcctattccg aaacctggaa gcacagcctg
     1081 cccgaggatg accagccggt cagcgatgcc gggcagaagg gccgggtggt cggcccccag
     1141 gccatcgccg ggcagctcgc ccgggtgttc acccacccgg ttccgatcac ctcggtccac
     1201 cattgtcacg ccgccgagat cgaattcctt tcggataccg aggccaaggg catctggtcg
     1261 atggaagaca gcctgtggtg gcagaacggc gcggtggagg agcacctcca cggctacggc
     1321 cattattacg agaccttccg caaggtggat ggccgctggc tcttcacatc gcgccagctg
     1381 atccggctcc gcgtcgacat gacgccggat ttctggtcgc ggctcaacat gtaggtcggg
     1441 ccggtcggct ggcgccggac ctcagatttc cacctggctg cccagttcga tgacccggtt
     1501 cgtgggcagc ttgaagaact gcattgcgct ttcggcattg cgcaccatcc acgcgaacag
     1561 cttctcccgc cagaccgcca tgccccccgg gcggctcgac gagatcagcg tctcgcgggc
     1621 aaggaaatag ctcgtttcag ccggcttgaa cgggccgccg gcgcggtctt cggcctggag
     1681 ttcggccggg atgtcgacct cgtccatgaa cccgtagttg agcacgatcc ggtagaaccc
     1741 gtgcccgagg tcttccacca tccggcggtc tccgggcgcg acatggggca cgccctttgt
     1801 cgcgacagtg aggatcgcca cgcgctggtg caggatgtgg ttgtgcttga ggttgtgcag
     1861 cagcgcatgg gggatgccct cgacacttgc tgcgaggaac accgagctac ccgccacccg
     1921 gcgcacccgg tccgccacgg attgtaggaa cacgtcgacc ggcatggcat cctcggccag
     1981 ccgctggcgc atcagccgcc gaccgctcgc ccaggtggtg agaagcgtga aggccacggc
     2041 cgcgaccagc agggggaacc agccgccatc ggggatcttg gtcacgttcg aggcgaagta
     2101 gaccccgtcg acggcaagga aggcgccggt caccactgcc gccagcagcg gatgccactt
     2161 ccacacggcg aaagtcagca cgcccagcat gcaggcggtg atgaacatcg tgccggtcac
     2221 cgcgatgcca taggccgcgg ccaggttgct cgagcttcgg aagcccagga ccagcaggat
     2281 gacgaggatc agcaggcccc agttgaccag cggcacatag atctgccccg ctgcccgggc
     2341 gctggtgtgc gcgatccgca ggcgcggcag gtagccgagc tggaccgcct gctgggaaac
     2401 cgaataggcg cccgagatca ccgcctggct ggcgatgatc gtggccagcg tggccaggat
     2461 caccagcggc agccgcgacc attccggcgc catcaggaag aacgggttct ccaccgctgc
     2521 cgggtttttc agcaatagcg ccgactggcc cagatagttg agcagcaggc agggcatggc
     2581 aatatagagc cacgacacca tgatcgcctt gcgcccgaag tggcccatgt cggcatagag
     2641 cgcctcggcc ccggtcaccg ccagcaccac cgagccgagc gccaggaagg cgagcttcgg
     2701 gtcgatcagg aagaagtcga gagcatagcg ggggctcagc gcgccgagca cctgcggcgc
     2761 gtgggcgatg ttgacgaggc ccagtacggc cagcaccccg aagtaaacga tcatgaccgg
     2821 gccgaacagg cggccgacca ccgccgtgcc gtgcgactgg atcgcgaaga ggccgacgag
     2881 gatcccgatc gagagcggca ggacccagta gccgaagctg tcgttcacga ccgtcagccc
     2941 ctccaccgcc gagagcaccg agattgccgg agtgatgatc gcatcgccat agaacagggc
     3001 cgtggcgatc acgcccagca tgccgatgat cggcgtccac cgtgtaatgc ccagcttgcg
     3061 cccgatcagc gccagcagcg ccaggctgcc gccctcgcca tggttgtcgg catggaggat
     3121 gacgaagacg tacttgatcg tgacgatcag catcatcgtc cagaatatca gcgacaacac
     3181 cccgaagatg tgcgccggat cgaccgtcag cgggtgatgg ccgataaagc tttccttgag
     3241 cgcgtagagc ggagaggtgc cgatatcgcc gaacaccacg ccgattgcgc cgatggccag
     3301 ggtggcaagg ctgtcgtgat gatcgtgccc ggtggcgggc tcgctggctg attcgctcat
     3361 gatctggcgg ctagaagcgc cggctggcgc ccgccatgac ttttacgcaa tgtttatgtc
     3421 ccacccgatt tctcgggtcg aaatttaacg gccatcaagc ccagatcagc ctcgcaacat
     3481 ttgcgggagc cataccgtgc tatcgatcgt caaacggata agcggcgggc gagggcgcct
     3541 cccctgggaa ctggcgctac gccttggcgg ggtcggcgtg atcgcgggcg cctggctggt
     3601 cgcccgctcc ttgtgccgct ggtccgccat gccccggcca cacccggcaa cctttctgga
     3661 attcgccatg accaccgccg ccttcgcctt gctgacgacg gggctcgcct ttctcgtcga
     3721 aggaccgggg ctgttccggc aagttcccat tccccgccac agcgccttct tccccgcatc
     3781 accagcgcat tgaccgcact gcgctctcga cagcgacgcc gaactgggcg caaaccgcgg
     3841 tggatggatc gggatacccg tcagaacagg ccccccaagg caggtggggc atggggctcg
     3901 ccgggcgggt atgtcgccgc gctggcgctg gtcgtgattg tcaccctggc cggattggcc
     3961 atcgtctctc tcggcaacat cgccaatatc ggattgctgt tcctcttgcc cgtcatggtc
     4021 gcggccaccc gctacggcct gcgcaccggc atcgtgacca gcctcgcgtc gtcgctggcc
     4081 tataattttt tcttcatccc gccgacccac accttcacga tcgagaaccc gcagaacatc
     4141 atcaccgtca tggtcctgct gggcgtcgcg gtcgccgcca gccagctcgc cgcgcaggtt
     4201 cgccagcagg cgctgctggc ccaggccagc gccgcccgca accaggcgct cgccggcttc
     4261 gcccgccagt tgactggcat cgggacggcg gaggccctgg ccgaggtctg ctgtacccag
     4321 gccagccgcc tgctggaggt aaacgccgtc ctgctcatgc cgtccggcca ggggctggaa
     4381 gtccgctccg ccgcgccgcg ccctgccgaa ctcgaattcc tcgaccatgc cgccgcccgc
     4441 gcctgcttcg atacggccca gcccgccggg cgtagcaccg acagcctgac cgcgtccgaa
     4501 tggcagtttc acccgatcac cggggccggc cacgtgcttg ccgtgatcgg cctgtcccgc
     4561 gccgatgcgg gcgatccggt cgccgccgat cagctgccgc ttctccttgg catgatcgat
     4621 caggcaggcc tcgccctggg gcgcattgcc ctgaccgacg acatggccgc gatcgcccag
     4681 gtgcgcgagc gcgaccgcct gcgtcacgcc ctgctctcct cggtgagcca cgacctgcgc
     4741 accccgctca ccacgatcat cggcacgctc aaccaattgc gcccggccga tgccgaacag
     4801 gccggccagc tggctgaaat gagggtggag gccgaacgcc tgcaccggtt cgtcgccaat
     4861 ctgctcgaca tggtgcggat cgaggccggc gcgctgcgcc tggcagtaga gccggtcgac
     4921 ctgtccgatg cggtggccag cgcgctgcag gacatggacc gcgcccttgc cggacagccc
     4981 gtcaatgtcg cgatcccgcc cgagctgccg ctggtccagg tcgatccgca gctgttccac
     5041 cactgcctga tcaacctgat cgacaatgcc gccaagcacg gcatgtcctc cggcccgata
     5101 gccatctcgg cgcggcggga ggagcacggg gtggttctct cggtcgccga tgaaggcccg
     5161 ggcattccgg cagggcagga acagcgcatc ttcgaaacct tcacccgcct cgaaggctcc
     5221 gaccgcaagg gcggcacggg cctcgggctc gccatcgtca agggcttctg cgaggcgatg
     5281 gggctggccg tggccgccgc caaccgggct gaccggccgg gcgcctgctt ttccatccac
     5341 tttccggcca ctcgtctcaa ggagctacct ggagcatgac cattccggtg accgtgctgg
     5401 ccgtcgacga cgaggccgcg atccggcggc tgctccacgc cagtctcgat cgcgccggct
     5461 accgtgtggt cgaggcgggg acggcgcgcg aaaccattgc cgcgctccag atcgacaagc
     5521 ccgatctcgt cctgctcgac cttggcctgc cggatcgcga cgggcttgaa ctcattcccc
     5581 tgatcaagca ggccggagcc gccatcatcg tgatctccgc ccgcgatgcc acgcagcaga
     5641 aggtcgccgc gctggacctc ggtgccgacg actacgtctc caagccgttc gacagcgagg
     5701 aagtcctcgc ccgcatccgc gttgccctgc gccaccgtct ggccggcgaa accgacgtgc
     5761 cccgtctacg tcgcggaccg gtcgagatcg accttgccgc acgcatggtc cgccgggatg
     5821 gcgaagaggt ccacctgacc ccgcgcgaat atggctttct cgccgaactg gccaaggccc
     5881 cgggccgcgt cgtcacccac gcccagctgc tgcgcgccgt ctgggggccg ggccacgaaa
     5941 ccgatgtcga atacctgcgc gtcgccgccc gcagcatccg ccgcaagctg gaggcggacc
     6001 ccagccgccc gcgcctgttc cgaaacgaac ccggagttgg ctaccggctc atgggggaag
     6061 agtaagcagg ggcaggagca ggcaggggca gggaaatggt cggggagaca ggattcgaac
     6121 ctgcgaccct ctgctcccaa attagaagtc gcacccacgg cattctgcga cg
//
LOCUS       DATNBV010000433        11341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3066541,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000433 DATNBV010000000
VERSION     DATNBV010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11341
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3066541"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2075)
                     /locus_tag="VN627_10275"
     CDS             complement(<1..2075)
                     /locus_tag="VN627_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXD03681.1"
                     /translation="MSRLFPLQDNQREAVDPRQTVWLSASAGTGKTQVLSARVLRLLL
                     QDEVHPSQLLCLTFTKAGAAEMATRVNEVLADWVRMPDDQLAIQLAHIGAPTNPATRA
                     RARTLFAAVLDCPGGGLRINTIHAFSQWLLAGFPEEAGLTPGTRAMEDRDRAVLSREV
                     LSALLLEAEERGDTARLDALAKLSLRMGPEDVERFLLRCADCREAWFGTGSWAPPLRP
                     RVERLLGLPEGASLAELCEDSAFDVRSLRRCMEINADWPAATGQKNANAIGDWLALDP
                     AARGEAIEALAGVFLTKEGEQRASKSQDKIDPSYSSHVERVLDSIHPVRQRKAMLDLA
                     AWLTPALDLGRAFALAWDEAKQREGLIDFDDQIRQAADLLNRSDLAEWIRYKLDRRFD
                     HILIDEAQDTNAAQWQIIDALTGDFFAGAGQHGAEALRTLFVVGDYKQAIFRFQGTSP
                     ENFEAARERVRQSMAGAAANALQLRSGPDARTLQELGLERSYRTARPILDYVDRTIAH
                     VGHSRFGLKDLPESHVGQERPGTVTLWQPVGLGRDEQELAEDQPEEGEQSWLSRPDRQ
                     LADRLARQIAFWLDTRNPGYPLVKGHHRRAEAGDIMVLVRKRKELAGLIVARLHARGV
                     PVAGVDRLRLGAPLAVQDLISALRIALQPLDDLTLAGLLVSPLLGWTQEQLLEHGWRE
                     KGVALWDHLR"
     gene            complement(2072..5137)
                     /gene="addB"
                     /locus_tag="VN627_10280"
     CDS             complement(2072..5137)
                     /gene="addB"
                     /locus_tag="VN627_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HXD03682.1"
                     /translation="MSERPSERPGPSLYSIAAHRGFADALVAGLVPRYGDGQLGLARL
                     TLLLPSRRAVRTVTEAFIRHAGEQGGGQAGLLLPRMAVVGDLDLDETLGPLLDPLGAG
                     LDIAPAADPVRRWLRLAAFLREEQGDKAPSGPALLRHAFEIARTMDRLLVEGIAPEDL
                     LAERVIGVVGEQAAHWQSSIRLFARTRARWVAELAERGEIDAPERRNRLFEAAGRAWR
                     ESPPAHPVVAAGITSASPALARLLRVVAETPRGAVILPDLDLALDDEIWDSLGTAGAP
                     AELGDPPIGRTDAVTNPQYHLKLLLNRMGVARGEFEPWHRSGLSPAPPARSRAISNLF
                     LPPEASARWVRLKADDRRLAGVRLMESAHPGEEAQAIAILIREALEVPERRVALVTPD
                     RALAARVIAHLGRWNIAADDTAGQPLSATVAGRLFLQLAEVAATAAAPVQLVALLSHP
                     LAGTGGLPGWEGSRADWLTNARRLDQALRGPRPEPGFGHIRAAVVATKNPRLAAWWEA
                     VEDLLRPLLGSAEAPLADLLDRLATAAEALSAGTIWGGADGRTLAGFVDGLRSAAGDA
                     GTSLTPEDLPTVLRDAMEREAVRPPWGGHPRVAIYGLIEARMSRADLVICGGLTEGTW
                     PGTSAPDALLAPAVLRALGVPGADFRIGLAAHDLAAALGAPEVVLSHARRDEAGPAIP
                     SRFVLRVRAMLGEKLAGEHAETEVVKLAREMDEPSVFAGAYPRPQPRPSAAQRDVPIA
                     VTGLDRLRGDPYQFYASDVLRLRKWDALDAEPTAAWKGTAVHAILERWHKEGGELLPI
                     AEAELAKMSAHPLVRGLWWPRLVEALKWVELEIARLAGEGRAVALSEARGDITFRGVK
                     IHGRADRIDRLPDGTLAVVDYKTGSPPSARRVEEGFALQLGLIGLMAREGAFKDAAGR
                     TVSGEPSRFEYWSLNRSRKSETGFGECSEPIPEGRRTAGIPRDEFLERTGEFLADAID
                     RWIKGDEPFTARLNPDLPGYTDYDQLMRLDEWQARAADLPGDGLAGDEA"
     gene            complement(5144..5872)
                     /locus_tag="VN627_10285"
     CDS             complement(5144..5872)
                     /locus_tag="VN627_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXD03683.1"
                     /translation="MSRLASDTAMVMAAGLGKRMRPLSTTRPKPLIPVGGKPLIEWSL
                     DALGKAGVSKAVVNAHYLADSLEAHLAGYGQRHPELAITVSDERELLLETGGGMVKAQ
                     GHLPDPFFCLNSDNIWLDGPRDAFTELSLAWDAARMDALLLVVRHPAAHCYEGLGDFH
                     LDPLGRISRRRPGRVAPFIYTGIQLVSHRLLRDAPEGPFSTNVLWSRAMAEGRLFGWV
                     HTGEWFEVGTPQAIGPTESLLSRG"
     gene            complement(5869..6852)
                     /locus_tag="VN627_10290"
     CDS             complement(5869..6852)
                     /locus_tag="VN627_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXD03684.1"
                     /translation="MSGYIPDAVEPFLREAGWAGAQIDPLPGDASFRRYFRLKQRERT
                     AMLMHAPPPNEDPAPFLRAARWLENSGLRPPHVLHEQADHGLVLLEDFGDVRMRDYLD
                     DWAQDELEVYTAAVDTLTALHRVPPGPFTAYSLSEYQREAKLFIDWYCPAQNLYVDGK
                     GWTEAWDQVLTPMLGRQRPGVVVLRDYHAENIMLLGALHKQGLLDFQDALVGHPAYDL
                     VSLLQDARRDVSPELEEAMLAHYLKGRADPETFRADYARLGAQRNAKIVGIFVRLWKR
                     DGKPRYLDLIPRVWALLERDLAHPALAPVAAWFDANVPAELRQAGGGAFNP"
     gene            complement(6849..7295)
                     /gene="tsaE"
                     /locus_tag="VN627_10295"
     CDS             complement(6849..7295)
                     /gene="tsaE"
                     /locus_tag="VN627_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXD03685.1"
                     /translation="MSTRLLPDLAAMAAYGARIAEVLRPGDVVALSGGLGAGKTTLAR
                     AILAGLGHEGEVPSPTFSIIELYDSPGVRLPVIHADFYRLKDPSETEELGLDDYRADA
                     ALIAEWPEMAGGFAHEPGCLSILLESAGEGRIAIASAGRDWVGRIP"
     gene            complement(7292..9649)
                     /locus_tag="VN627_10300"
     CDS             complement(7292..9649)
                     /locus_tag="VN627_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HXD03686.1"
                     /translation="MTFTPTALALGGLLLAAWALVAAWFVLAARAREKRAETALRNAR
                     RLARMVEESPAIPLLVRADGRIEASGRLARWLGLERVPQYLSELKEDEGGANGGGGIP
                     VLQLGELNDLVRRTQKTGAPFRMVVTPSGSRRSLSLRGQLADPQISPGGAALVWVFDF
                     SESEGELIRLRDDALRARADFTALVGLIEAAPMPMWFRDRQGRLRLVNRAYVEAVQGE
                     SAAGVVEAQTELVEAVDGLSAAQVALQAAQMERPIERMVPATINGQRRTLRLSDLPLG
                     AEGIAGYAVDIEDMEELARSLRAFREAQRAMLDQLSAGVAQFDAARRLVFANQPFQRI
                     FALKPTVMLDPPPFERLLDMARDAGRVPEARDFPVWRREKAAWFRDSVAHEEAWPLPD
                     GTHLRVVAQPMPDGGLLLIAEDRTEELRISANRDTLLRTRTATFDSLFESLGVFAPDG
                     RMQLWNRRFAQDWGLDGEFLDTHPKIDLLLERIASRLLKPAQARLVGDVVRAATLDRK
                     QTGGRAVLADGRTLEFAGVPLPDGNGLLTVLDITDSQKAEAALRERNAALLEADAVKT
                     RFLANMSYEFRTPLTSIGGFAELLEAGVGGELNESGRDYVAAILSSVHTLGEQIETVL
                     DLSQSEAGLLPLAEEEIELLPFVTKLVEERAEAVGEGGLTLDLRGDKSAGRVKGDRRR
                     LGRVIGHLIDNAIAATPRGGRILVDLAPTAEGPRIVVSDNGPGMDSATLARALEGLKS
                     GPTGNHDRRRGLGLPLARRIIEAHGGRFELLSEPGQGTAAIIQLP"
     gene            complement(9736..11148)
                     /locus_tag="VN627_10305"
     CDS             complement(9736..11148)
                     /locus_tag="VN627_10305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXD03687.1"
                     /translation="MEQTVGGFSVRHSRIIAILAAIAAVLAAVRVFIIGSHYPFDYNE
                     GWNAFHAQIAVSGQALYPGSDALTTTNYPPLSFYLIGVLGRLVPDLIVVGRIVALLSA
                     TVVGWCIFRIVRAFGTGPAGPLAGLALFAMINATAFRGYLAMNDPQWLGQAFMAGALL
                     LLVTGSAARPLATLTTVAAALLVVVGISVKHNIVAIPAACTLWLLLNDRRALAVWVAT
                     GAAGCLALLLIDRLVFGGTMMPSILFQPRSYSAMRALGHGAVGLLFVPAGVLAVKAHP
                     RIADRRYELVLYGLAISAVIGFVEGSGAGVDINAWFELLIFGAIAFGIALPVLAGEKG
                     FAWKFRFGIAPMVALVPYGLYLVAEDLGGRAERSAQTRAIVAQVAAVDGKVACDMLAY
                     CYWAGEDYGLDFFQVGQRAATATDSAELEQHLAAGDFAAFLMGDWGTAPPPGPVQAFA
                     LQHHYQPAFRDAGTVLYRRD"
     gene            complement(11148..>11341)
                     /locus_tag="VN627_10310"
     CDS             complement(11148..>11341)
                     /locus_tag="VN627_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03688.1"
                     /translation="LATTLFNLAYSIAVPYYTFDWAISASFIFGVLSIMHNKRIVPKI
                     SLVILALHLGVIAAIRTFS"
BASE COUNT         1871 a         4194 c         3722 g         1554 t
ORIGIN      
        1 cgcaagtggt cccacagcgc cacccccttc tcgcgccagc cgtgttcgag caactgctcc
       61 tgcgtccacc ccagcagcgg cgaaaccaga agcccggcaa gtgtaagatc gtcaagcggt
      121 tgcagcgcaa tcctcagggc cgatatgagg tcctgcaccg ccagcggcgc gcccagccgc
      181 aatcggtcga cgccagccac cggcacgccc cgggcatgca gccgcgccac gatcagcccg
      241 gccagttcct tgcgcttgcg caccaggacc atgatatcgc ccgcctcggc gcggcgatga
      301 tggcccttta caaggggata tccaggattc ctggtatcca gccagaaggc gatctgccgc
      361 gccagccggt ccgcgagctg gcggtccggg cgcgagagcc acgactgttc gccctcctcc
      421 ggctggtcct cggccagctc ctgctcgtcg cgtcccaggc ccaccggctg ccacagggtc
      481 accgtgccgg gccgctcctg gccgacatgg ctctccggca agtccttgag accaaagcga
      541 gaatggccaa catgggcaat cgtgcggtcg acatagtcga ggatcggcct ggccgtgcgg
      601 taggaccgtt ctaggccgag ttcctgcaag gtccgtgcat cgggaccgga acgcagctgc
      661 aaggcatttg ccgccgcccc cgccatcgac tggcgcaccc gctcgcgcgc cgcctcgaaa
      721 ttctccgggc tggtcccctg gaaccggaag atcgcctgct tgtagtcacc caccacgaac
      781 agtgtgcgca gcgcttcggc gccatgctgc ccggcgcccg caaagaagtc gccggtcagc
      841 gcatcgatga tctgccactg ggcggcgttg gtgtcctgcg cctcgtcgat caggatgtga
      901 tcgaagcggc ggtccagctt gtagcggatc cactcggcaa ggtccgagcg gttgagcagg
      961 tctgcggcct ggcggatctg gtcgtcgaaa tcgatcaacc cctcgcgctg cttggcctcg
     1021 tcccaggcca gcgcaaaggc gcggcccaga tcgagcgccg gggtcagcca cgctgccagg
     1081 tccagcatgg ccttgcgctg gcggaccgga tggatggaat cgagcacgcg ctcgacatgg
     1141 ctggaatagg aggggtcgat cttgtcctgt gatttgctcg cccgctgctc gccctccttg
     1201 gtgaggaaga cgcccgccag cgcctcgatc gcctcgcccc gcgccgccgg gtcgagcgcc
     1261 agccaatcgc cgatcgcatt ggcgttcttc tgcccggtgg cggccggcca gtccgcgttg
     1321 atctccatgc accggcgcag gctgcgcacg tcgaacgcgc tgtcctcgca cagctcggca
     1381 aggctcgccc cttccggaag gcccagcagg cgctccaccc gtggccgcag cggcggtgcc
     1441 cacgagccgg tcccgaacca cgcctcccgg caatcggcgc agcgcagcag gaagcgctcg
     1501 acatcctccg gccccatccg cagcgacaat ttcgccagcg catcgagccg cgccgtatcg
     1561 ccccgctcct cagcctccag cagcagtgcg gaaagcacct cgcggctgag cacggcccgg
     1621 tcgcggtcct ccatcgcccg ggtgcccggg gtgagcccag cctcctcggg aaacccggcc
     1681 agcaaccact gcgaaaacgc gtggatcgtg ttgatgcgca gcccgccccc cgggcagtcg
     1741 agcacggcgg cgaacagcgt gcgtgcccgg gcccgggtgg cgggattggt cggcgcgccg
     1801 atgtgggcca gctggattgc cagctgatcg tcgggcatgc gcacccagtc agccagcacc
     1861 tcgttgacgc gggtcgccat ttccgccgcg cccgccttgg tgaaggtcag gcacagcagc
     1921 tgcgagggat ggacctcgtc ctgcaagagc aggcgcagca cgcgggcgga cagcacctgc
     1981 gtcttccccg tccccgccga ggccgagagc cacaccgtct gccgcggatc gaccgcctcg
     2041 cgctggttgt cctgcagggg aaacagccgg ctcatgcctc atcccccgcc aaaccgtccc
     2101 cgggcaggtc agccgcccgg gcctgccact catccagccg catcagctgg tcgtagtcgg
     2161 tatagcccgg cagatccggg ttgagccgcg ccgtgaacgg ctcatccccc ttgatccacc
     2221 ggtcaatcgc atcggccagg aattccccgg tccgctcaag aaactcgtcg cgcggaatgc
     2281 ccgccgtgcg ccgcccttcc gggatcggct cgctgcattc accaaatccg gtctcgctct
     2341 tcctggaacg gttgagcgac cagtattcga accggctcgg ctcccccgac acggtgcgcc
     2401 ccgccgcgtc cttgaaggca ccctcgcgcg ccatcagccc gatcaggccg agctggagcg
     2461 cgaagccctc ttccacccgc cgcgcgcttg gcggggagcc ggtcttgtag tcgaccacgg
     2521 ccagggtccc atcgggcagg cggtcgatcc ggtcggcccg gccgtggatc ttcacgcccc
     2581 gaaaggtgat atccccccgc gcctcgctga gcgcgaccgc ccggccctcg cccgccagcc
     2641 gggcgatttc cagctcgacc cacttgagcg cctcgaccag ccgcggccac cacagcccgc
     2701 gcaccagcgg atgcgcgctc atcttcgcca gctcggcctc ggcaatgggc agcagttcgc
     2761 cgccttcctt atgccagcgc tccagaatcg catggaccgc cgtgcccttc caagccgcag
     2821 tcggctcggc atcgagcgcg tcccacttgc gcaggcgcag cacgtcgctg gcatagaact
     2881 ggtagggatc gccgcgcagc cggtcgagcc cggtaacggc aatcggcacg tcgcgctggg
     2941 cggcggaagg gcgcggctgc gggcgcggat aggctccagc gaagacggac ggctcgtcca
     3001 tctcccgcgc cagcttgacc acctcggtct cggcatgctc gcccgccagc ttctcgccca
     3061 gcatggcccg caccctgagg acaaagcgcg aggggatcgc cgggcccgcc tcgtcccgcc
     3121 gcgcgtggct gagcaccacc tcgggcgcgc cgagcgccgc ggcaaggtcg tgcgcggcaa
     3181 ggccgatgcg gaaatcggcc cccggaacgc cgagcgcacg cagcacggcc ggcgccagca
     3241 gggcgtcggg ggcgctggtc ccgggccagg tgccctcggt caggcccccg cagatgacga
     3301 gatcggcccg gctcatccgc gcctcgatca gcccgtagat ggccacgcga ggatgcccgc
     3361 cccacggcgg gcgcacggcc tcgcgctcca tggcatcgcg caggaccgtg ggcaggtcct
     3421 cgggcgtcag gctggtcccc gcatcgcccg ccgcagagcg caggccatcg acaaaaccgg
     3481 cgagtgtccg cccatccgcc ccgccccaga tcgttcccgc cgaaagcgcc tcggcggcgg
     3541 tcgccaggcg gtccagcaga tcggccagcg gggcttccgc gctgcccagc agcgggcgca
     3601 gcaagtcctc gaccgcctcc caccacgcgg caaggcgcgg gtttttcgtg gcgacaacgg
     3661 cagcgcggat atggccgaag cccggctccg gccgcggccc ccgcagcgcc tggtccagcc
     3721 ggcgggcatt ggtcagccag tccgcgcgcg agccctccca gcccggcagg cctcccgtgc
     3781 ccgccaaggg atggctgagc agggccacca gctgcaccgg cgccgcagcc gttgcggcca
     3841 cttccgccaa ctgcaggaac agccgccccg caacggtggc ggacagcggc tgccccgccg
     3901 tatcgtccgc cgcgatgttc caccggccaa ggtgggcgat cacccgcgcc gccagcgccc
     3961 ggtccggcgt caccagagca acgcggcgtt ccggcacttc cagggcctcg cggatcagga
     4021 tggcaatggc ctgggcttcc tcgcccgggt gcgcgctttc catcaggcgc acgcccgcca
     4081 accgccggtc gtccgccttg agccgcaccc accgcgcgct cgcctcgggg ggcaggaaca
     4141 ggttggaaat ggcccggctg cgtgctggcg gcgctggcga caggcccgaa cggtgccagg
     4201 gctcgaactc gccgcgcgcc acgcccatgc ggttgagcag gagcttgagg tgatactgcg
     4261 gattggtcac cgcatcggtc cgcccgatcg gcggatcgcc aagctcggcg ggcgcgcccg
     4321 cagtgccaag cgaatcccag atctcatcat ccagcgcgag atccagatca ggcaagatca
     4381 ccgccccgcg cggcgtttcg gctacaaccc gcagcagccg ggccagcgcg ggcgaggcgc
     4441 tggtaatccc ggcggcgaca accggatggg ccggcgggct ctcgcgccag gccctgccgg
     4501 cggcctcgaa cagccggttg cgccgctcgg gcgcatcgat ctcgccccgc tcggccagtt
     4561 cggccaccca acgggcgcgg gtgcgggcaa acaggcggat cgagctctgc cagtgcgccg
     4621 cctgttcgcc gaccacgccg atcacccgct cggccagcag atcttccggc gcgattcctt
     4681 cgaccagcag ccggtccatc gtccgcgcga tctcgaaggc atggcgcagc agcgccgggc
     4741 ccgaaggcgc cttgtcgccc tgctcctcgc gcaggaacgc cgccagcctc agccagcggc
     4801 gcactggatc ggcagcgggg gcgatatcga gcccagcccc cagcggatcg agcagcggcc
     4861 ccagcgtttc atcgagatca agatcgccga ccaccgccat gcgcggcaac aacaagcctg
     4921 cctgaccgcc gccctgctcc cccgcatgac ggatgaaagc ctcggtaacg gtgcgcaccg
     4981 cgcggcggct gggcagcagc agcgtcagcc gggcgaggcc caattgcccg tcgccatagc
     5041 gcggcaccag ccccgccacg agcgcatcgg caaacccccg gtgggcggcg atggaataga
     5101 ggctggggcc tggtcgctcc gaaggccgct cgctcacgcc cgctcagccc cgcgagagca
     5161 gggattcggt cggcccaatg gcctgcggcg tgccgacttc gaaccattcg ccggtatgga
     5221 cccagccgaa cagccgcccc tcggccatcg cccggctcca cagcacgttg gtcgaaaacg
     5281 gcccctccgg cgcatcgcgc agcaggcgat gggaaacgag ctggatgccg gtatagatga
     5341 acggcgccac ccggcccggg cggcgccggc tgatccgccc cagcggatcg aggtgaaagt
     5401 cgcccagccc ctcataacag tgcgcggccg ggtggcgtac caccagcagc agcgcatcca
     5461 tccgcgccgc gtcccaggcc aggctcagct cggtgaatgc atcgcgcggg ccgtcgagcc
     5521 agatattgtc ggaattcagg cagaagaacg gatcgggcaa gtgcccctgc gccttgacca
     5581 tgccgccgcc agtttccagc aacaactccc gctcgtccga aacggtgatg gcgagctccg
     5641 gatgacgctg gccatagccg gcgagatgag cctccaggct gtccgccagg tagtgcgcgt
     5701 tgaccaccgc cttgctcacc cccgccttgc ccagggcatc gaggctccac tcgatcagcg
     5761 gcttgcctcc caccgggatc aacggcttgg gccgggtggt gctgagcggc cgcatccgct
     5821 tgcccagccc ggcagccatg accatcgccg tatcggaagc gagccggctc atggattgaa
     5881 cgcgccgccg cccgcctgcc gcaactccgc cggcacattg gcatcgaacc acgccgccac
     5941 cggggccagc gccggatggg ccaggtcgcg ctccagcagg gcccagacgc gggggatcag
     6001 atcgaggtag cgcggcttcc cgtcgcgctt ccacaggcgc acgaagatgc cgacgatctt
     6061 ggcattccgc tgcgcgccaa gccgggcata gtcggcccgg aaggtctcgg gatcggcccg
     6121 ccccttgagg taatgggcga gcatcgcctc ctccagctcg ggcgagacgt cgcgccgcgc
     6181 atcctgcagc agcgagacga ggtcataggc cggatggccc accagcgcgt cctggaagtc
     6241 gagcaggccc tgcttgtgca gcgcgccaag cagcatgatg ttctcggcat ggtagtcgcg
     6301 caggaccacc acccccgggc gctggcggcc aagcatgggg gtgagcacct ggtcccacgc
     6361 ctcggtccag cccttgccgt ccacatacag gttctgcgcc gggcagtacc agtcgatgaa
     6421 cagcttggcc tcgcgctggt attcgctcag cgaataggcg gtgaacgggc ccggcgggac
     6481 gcggtggagc gccgtcagcg tgtcgaccgc ggcggtatag acctccagct catcctgcgc
     6541 ccagtcgtca agatagtcgc gcatgcgcac gtcgccgaaa tcttccagca ggacgaggcc
     6601 atggtctgcc tgctcgtgca gcacgtgcgg cgggcgcagg ccggaattct ccagccagcg
     6661 ggccgcgcgc aggaacggcg ccgggtcctc gttgggcggc ggggcatgca tgagcatggc
     6721 cgtacgctcc ctctgcttca ggcggaaata gcggcggaac gaggcatcgc cgggcagcgg
     6781 atcgatctgc gccccggccc agccggcctc gcgcaggaag ggttcaaccg catcgggaat
     6841 ataaccactc atggtatccg ccctacccaa tcgcgccccg cgctcgcaat ggcgatgcgg
     6901 ccctcgcctg cactttccaa caggattgaa aggcagcccg gctcatgagc gaaaccgccc
     6961 gccatttcag gccattcggc gatcaatgcg gcatcggcgc gatagtcgtc gagcccgagt
     7021 tcctccgttt cggacgggtc tttcagccga taaaaatcgg catggatcac cggcagccgc
     7081 acccccgggg aatcgtagag ctcgatgatc gaaaaggtgg gcgacggcac ttcgccctca
     7141 tgccccagcc cggccaggat cgcccgcgcc agcgtggtct tgcccgcgcc gagcccgccc
     7201 gagagcgcca ccacgtcgcc cggccgcagc acttcggcaa tccgcgcgcc ataggccgcc
     7261 atcgcggcaa gatcgggaag cagccttgtg ctcacggcaa ctggatgatc gcggcagtgc
     7321 cctgccccgg ctccgacagc agctcgaacc gcccgccatg cgcctcgata atccgccggg
     7381 cgagcggcag gcccagcccg cgacgccggt catgattgcc ggtgggcccg gacttgagcc
     7441 cctccagcgc ccgggccagc gtggcggaat ccatccccgg tccattgtcg gaaaccacga
     7501 tgcgcgggcc ctcagcggtg ggcgcaagat ccaccagaat gcgcccgccg cgcggcgtgg
     7561 cggcaatggc attgtcgatc aggtggccga tcacgcgccc cagccgccgc cggtcgccct
     7621 tgacccgccc cgcactcttg tcgccgcgca gatccagcgt cagcccaccc tcgcccaccg
     7681 cctcggcccg ctcctcgacg agcttcgtca cgaacggcag cagctcgatc tcctcctcgg
     7741 ccagcggcag caagccggcc tcgctctgcg acaggtccag caccgtctcg atctgctcgc
     7801 ccagcgtatg aaccgaagag aggatcgcgg cgacatagtc ccgcccgctc tcgttcagct
     7861 cgccgccgac cccggcctcc agcaactcgg caaagccgcc gatcgaggtc agcggcgtgc
     7921 ggaattcgta gctcatgttg gcgaggaacc gggtcttcac cgcatcggcc tcaagcagcg
     7981 ccgcattgcg ctcgcgcagc gccgcctcgg ccttctggct gtcggttatg tcgagcacgg
     8041 tcagcaggcc gttgccatcg ggcagcggta ccccggcaaa ttccagcgtg cgcccgtcgg
     8101 ccaggaccgc ccggccgccc gtctgcttgc ggtccagcgt cgccgctcgc accacgtcgc
     8161 cgacgagcct ggcctgggcc ggcttgagca gccgcgaggc aatccgctca agcagcaggt
     8221 cgatcttggg atgagtgtcg aggaactcgc cgtcgagccc ccagtcctgc gcgaaacggc
     8281 ggttccacag ctgcatgcgg ccatcgggcg caaagacgcc gaggctctcg aacaggctgt
     8341 caaacgtggc cgtgcgggtg cgcagcaggg tgtcgcgatt ggccgaaatg cgcagttcct
     8401 cggtccggtc ctcggcgatc agcagcaggc cgccatccgg catcggctgg gcgacgacgc
     8461 gcaggtgcgt gccgtcgggc agcggccagg cttcctcgtg agccacgctg tcgcggaacc
     8521 aggccgcctt ttcccgccgc cacaccggaa aatcgcgcgc ctcgggcacc cgtccggcat
     8581 cgcgcgccat gtcgagcagc cgctcgaacg gcggcggatc aagcatgacg gtcggcttga
     8641 gcgcgaaaat ccgctggaac ggctggttgg cgaagaccag tctgcgcgcc gcatcgaact
     8701 gggcgacgcc ggccgaaagc tggtcgagca tcgcccgctg cgcctcgcgg aaggcgcgca
     8761 gggagcgggc cagctcctcc atgtcctcga tgtccaccgc atagccggcg atcccctcgg
     8821 cccccagcgg caggtcggac aggcgcaggg tccggcgctg gccgttgatc gtcgccggaa
     8881 ccatgcgctc gatcggccgc tccatctgcg cggcctgaag ggcaacctgc gcggcgctga
     8941 ggccatcgac ggcctcgacc agctccgtct gcgcctcgac caccccggcc gcgctctccc
     9001 cctgcaccgc ttccacatag gcccggttga ccagccgcag ccggccctgc cggtcgcgga
     9061 accacatcgg catgggcgcc gcctcgatca ggccgaccag cgcggtgaaa tcggcccggg
     9121 cgcgcagggc atcgtcgcgc aggcggatca gctcgccctc gctctccgaa aagtcgaaaa
     9181 cccagaccag cgcggccccg cccggcgaaa tctgcggatc ggccagttgc ccccgcagcg
     9241 agagcgaccg ccgcgagccc gaaggtgtga cgaccatgcg aaagggcgcg cccgttttct
     9301 gcgtgcggcg gacgaggtcg ttcagctcgc ccagctggag cacgggaatg ccgccaccgc
     9361 cattggcccc gccctcatcc tccttcagct cggagagata ctgcggcacc cgctctaacc
     9421 ccagccagcg cgccagccgc ccgctcgcct cgatccgccc atcggcgcgg accagcagcg
     9481 ggatcgccgg gctctcctcc accatccgcg ccagccgccg cgcattgcgc agcgccgtct
     9541 ccgcccgctt ctcgcgcgcg cgcgcggcca ggacgaacca cgccgccacc agcgcccagg
     9601 ccgccagcag cagcccgcca agcgccagcg ctgtgggggt aaaggtcatg ccgcttggct
     9661 atgcggcggg gcgggcgttg gcaagcagcg aaggcgccgg aaacgaacta tggcgcggcg
     9721 ctgaggcgcg ccggctcagt cgcgccggta aagcacggtg ccggcatcgc ggaacgcagg
     9781 ctggtagtga tgctgcagcg caaaggcttg gaccgggccg ggcggcgggg ccgtacccca
     9841 gtcgcccatc aggaaggccg cgaagtcacc cgccgcaagg tgctgttcca gctcagcaga
     9901 atcggtcgcg gttgcggcgc gctggccgac ttggaagaaa tcgagaccgt agtcctcgcc
     9961 cgcccagtaa cagtaggcaa gcatgtcgca ggccaccttg ccatcgacgg cggccacttg
    10021 cgcgacgatg gccctcgtct gcgcgctgcg ctcggcccgg ccgccgagat cctcggcgac
    10081 gaggtacagc ccgtagggca caagcgcgac catcggcgcg atcccaaacc ggaacttcca
    10141 ggcaaaccct ttttcgccgg ccagcaccgg cagggcgatc ccaaaggcaa tcgcaccgaa
    10201 gatcaggagt tcgaaccagg cattgatatc gacgccggct ccgctgccct cgacaaagcc
    10261 gatcaccgcg ctgatggcga ggccatacag caccaactcg tagcgacggt cggcgattcg
    10321 cggatgcgcc ttcaccgcca gcacccccgc tggcacgaac agcaggccga ccgcaccgtg
    10381 ccccagcgca cgcatcgcgg aataggagcg gggctggaac aggatcgatg gcatcatcgt
    10441 gccgccaaag acgaggcggt cgatcagcag cagcgcgaga catccggccg cgccggtcgc
    10501 cacccacaca gccagagcgc ggcggtcgtt cagcaggagc cagagggtgc aggcggccgg
    10561 aatggcaacg atgttgtgct tcacgctgat gccaacgacg accagcaagg ccgcggccac
    10621 agtcgtcaac gtggcgagcg gacgcgcggc gctgccagtg accagcagca gaagcgcacc
    10681 ggccatgaag gcctggccga gccattgcgg atcgttcatc gccaggtagc cgcggaaggc
    10741 cgtggcattg atcatggcga acagcgccag acccgccagg gggccggcgg gccctgtgcc
    10801 gaacgcgcgg acaatccgga agatgcacca gccgacgacc gtggcggaca ggagcgccac
    10861 gatccggccg accacgataa ggtccggcac gagacgcccc agcacgccga tcagatagaa
    10921 tgacagcggc gggtaattgg tcgtggtcag cgcgtcggag cccggataga gcgcctgccc
    10981 gctgaccgcg atctgcgcgt ggaaggcgtt ccagccctcg ttatagtcga atgggtagtg
    11041 cgatccgatg atgaacacgc gcaccgccgc gagcaccgcg gcaattgcgg ccaggattgc
    11101 gatgatccgg gaatgccgga ccgagaagcc gccgactgtc tgctccatca gctgaaggtg
    11161 cgaatggcgg cgatgacacc caggtgaagc gccaggatca ccagcgagat cttcggcacg
    11221 atccgtttgt tgtgcatgat cgagaggacg ccgaagatga acgaggccga gatcgcccag
    11281 tcaaaggtgt agtagggcac ggctatgctg taggccaggt tgaacagcgt ggtggcgagc
    11341 g
//
LOCUS       DATNBV010000434         2956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4079676,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000434 DATNBV010000000
VERSION     DATNBV010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4079676"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VN627_10315"
     CDS             <1..596
                     /locus_tag="VN627_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HXD03689.1"
                     /translation="REAHHFEHLARVVRPDLVESLAARQPVRIWSAGCSSGEETWSLM
                     MTLLGPDRQGGLELTRRDLKVLASDIAEHALAKAQAATYPAKDMAPVPEALLRPWTHA
                     AGENLVMTEEARGLVRFRHLNLQGEWPMNGRFQVIFCRNVMIYFDNPTKERLVERFAE
                     ALEPGGYLYIGHSERVTGAAAALLSPVGPTIYRRNQP"
     gene            593..1621
                     /locus_tag="VN627_10320"
     CDS             593..1621
                     /locus_tag="VN627_10320"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HXD03690.1"
                     /translation="MIRVLIIDDSATMRAILMSRLSAEHDISVIGTAANAAEGRELIK
                     RFNPDVVTLDIEMPGMNGLDFLEKIMTLRPTPVIVVSGSTQEGNEITARALALGAVDC
                     YAKSDRSGMLQLDDHGRLADLIREAAQVRFNNRWPVSPKVAAATRRIASAPTKTRLIA
                     IGSSTGGVEALQVLLKDFPENCPPTVIVQHVNPRFAPAIARTLDLVGPAKVQLAEPDM
                     PLRDGNIYLAPGGDRHMTIGGSSAYYARLRTGDPISGHVPSVDALFHSVAEVARNHAV
                     GVLLTGMGSDGAKGLLAMAQAGAHTIAQDEATCTVFGMPRAAISLGAASVVAPIGRIA
                     QHVLRQAA"
     gene            1621..2187
                     /locus_tag="VN627_10325"
     CDS             1621..2187
                     /locus_tag="VN627_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HXD03691.1"
                     /translation="MLVAPLNHGAAPPGPVHRITVLQGQARVSDRDDDELTTILGSCV
                     ATCLFDPVARIGGLNHFLLPEPPDSHDPAEVDVHYGVYLMEILINDMLRKGASKAQLR
                     AHLYGGANLRAGMAPIGTANAEFARAFLDRERIPLLREDLGGNQARRIEFRPATGKVR
                     CRVVENRLASEAAPPPRPTHRGGDVELF"
     gene            2205..2576
                     /locus_tag="VN627_10330"
     CDS             2205..2576
                     /locus_tag="VN627_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXD03692.1"
                     /translation="MLQPIRILTVDDSASMRALLRHALTGNGFDVVQAEDGVAALEWL
                     AGNEVDVVITDINMPRLDGFGLIEQLRCGSRHRDRPILVLTTESSDEKKLRARAAGAT
                     GWIVKPFDPDKLVAAVRRVAH"
     gene            2602..>2956
                     /locus_tag="VN627_10335"
     CDS             2602..>2956
                     /locus_tag="VN627_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXD03693.1"
                     /translation="MPRELITFEVEGQLFGLDVMAIREIRAWTPVTRLPGVPHYVAGV
                     VNLRGTVLPVIDLAARLGWEAVEPTPRHAIIVTQIGSRQTGLIVESVSDIVAINLDTL
                     QPPPSTADSNVVQFLS"
BASE COUNT          485 a         1090 c          915 g          466 t
ORIGIN      
        1 accgcgaagc tcaccacttc gaacatctcg cccgcgtcgt gcgcccggac ctggtggaaa
       61 gcctcgccgc gcgccagccg gtgcggatct ggtccgccgg ctgctcgagc ggggaggaga
      121 cctggtcgct gatgatgacc ctgctcggtc ccgatcgcca agggggcctc gagttgacgc
      181 ggcgcgacct caaggtgctc gccagcgaca tcgccgaaca tgccctcgcc aaggcccagg
      241 cggcgaccta tccggccaag gacatggcgc ccgtgcccga agccctgctg cggccatgga
      301 cccatgccgc cggggaaaac ctggtcatga ccgaggaggc gcgcggcctg gtccgtttcc
      361 ggcacctcaa tttgcagggc gagtggccga tgaatggccg cttccaggtg atcttctgcc
      421 gcaacgtcat gatctatttc gacaatccta cgaaggagcg cctcgtcgaa cgcttcgccg
      481 aggcgctcga acccggcgga tacctctaca tcgggcattc cgagcgcgtc accggggctg
      541 ctgccgccct gctcagtccg gtcggaccca ccatctaccg gaggaaccag ccatgatccg
      601 cgtcctgatc atcgacgatt cagccaccat gcgggcgatc ctgatgtcgc gcctttccgc
      661 cgagcacgac atctcggtca tcggcacggc cgccaatgcg gctgaggggc gcgagctcat
      721 caagcgcttc aatccagacg tggtgacgct ggatatcgag atgcccggga tgaacgggct
      781 cgatttcctc gagaagatca tgaccctgcg gccgacgccg gtgatcgtcg tgtccggctc
      841 gacccaggag ggcaacgaga tcacggcccg cgccctcgcc ctgggtgcgg tcgattgcta
      901 cgccaagtcc gaccggtccg ggatgctcca gctcgacgat cacggccgcc tcgccgacct
      961 gatccgcgag gccgcgcagg tccgcttcaa taaccgctgg cccgtctcgc ccaaggtggc
     1021 ggccgccacc cgtcgcatcg ccagcgcccc gaccaagacc cggctgatcg cgatcggttc
     1081 gtccacgggc ggggtcgagg ccctgcaggt tctgctcaag gactttcccg agaactgtcc
     1141 gcccacggtg attgtccagc acgtcaatcc ccgcttcgcc cccgcgattg cacgaaccct
     1201 cgatctcgtc ggcccggcca aggtccagct ggcggaaccg gacatgccgc tgcgcgacgg
     1261 caacatctat ctcgcccccg gaggcgaccg ccacatgacc atcggcggct cgagtgccta
     1321 ctatgcccgc ttgcgcaccg gcgatccgat ttccggccat gtgccgagcg tcgatgccct
     1381 gttccattct gtcgccgaag tggcgcgcaa ccatgcggtc ggcgtcctcc tcaccggcat
     1441 gggcagcgac ggcgcgaaag gcctgctggc gatggcccag gccggcgctc ataccattgc
     1501 ccaggacgag gcgacctgca ccgtcttcgg catgccgcgc gccgcgatct cgctgggcgc
     1561 ggcatcggtg gtcgccccga ttggacgcat tgcgcagcac gtcctgaggc aggccgcctg
     1621 atgcttgttg cccccttgaa ccacggcgca gccccgcccg gtcccgtgca ccggatcacc
     1681 gtcctgcagg ggcaggcccg ggtgagcgac cgcgacgacg acgagctgac cacgatcctg
     1741 gggtcctgcg tcgccacctg cctgttcgac ccggtagccc ggatcggcgg cctcaaccac
     1801 ttcctcctgc cagagccgcc cgacagtcat gacccggccg aggtcgatgt tcactacggc
     1861 gtctacctga tggagatcct gatcaacgac atgctgcgca agggcgccag caaggctcaa
     1921 ttgcgcgccc atctctatgg cggcgccaac ctgcgggccg gcatggcgcc gatcggcacg
     1981 gcgaacgccg agttcgcccg cgccttcctc gatcgcgaac gcattccgct gctgcgcgaa
     2041 gacctcggcg gcaaccaggc ccgccgtatc gagttccgtc ccgcgaccgg caaggtccgt
     2101 tgccgcgtgg tcgaaaaccg cctcgccagc gaggccgcac caccgcccag gccgacccac
     2161 cgcggcggcg atgtcgaact gttctgacga aagcgagacc agacatgctc cagcccatcc
     2221 gcatcctgac cgtggacgac agcgcctcca tgcgcgcgct gctgcgtcac gccctgaccg
     2281 gcaatggctt cgacgtcgtt caggccgaag acggcgtggc cgcgctcgaa tggctcgccg
     2341 gcaacgaggt cgacgtagtc atcaccgaca tcaacatgcc acgcctcgac ggcttcggcc
     2401 tgatcgagca attgcgctgc ggcagtcgcc accgcgaccg gccgattctc gtgctcacca
     2461 cggaaagctc cgacgagaag aagctgcgcg cgcgggcagc cggcgcgacc ggctggatcg
     2521 tcaagccgtt cgatcccgac aagctcgtcg cggctgttcg ccgcgttgcc cattgattcc
     2581 tgccaagcga aaggtgcgct catgccccgc gaattgatca cgttcgaagt cgaaggccag
     2641 ctgttcgggc tcgatgtcat ggccatccgc gagatccgcg cctggacccc ggtcacccgg
     2701 ctgccgggcg tgccgcacta cgttgccggc gtggtcaacc tgcgcggcac ggtcctgccg
     2761 gtgatcgacc tcgccgcccg gctcggctgg gaagccgtcg agccgacccc gcgccacgcg
     2821 atcatcgtca cccagatcgg cagccgccag accgggctga tcgtcgaatc ggtcagcgac
     2881 atcgtcgcca tcaacctcga cacgctgcag ccgccgccct cgacggcgga cagcaacgtg
     2941 gtccagttcc tctccg
//
LOCUS       DATNBV010000435         2707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3450872,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000435 DATNBV010000000
VERSION     DATNBV010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3450872"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VN627_10340"
     CDS             complement(<1..1059)
                     /locus_tag="VN627_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HXD03694.1"
                     /translation="MPRMRIATRFPSSMPIYGLEKSEVTLPGVSVAPLPDPAFQQILT
                     PEALGFLAELERKFGPERLRLLEARKERQRRLDAGEKPDFLKETAQIRAGDWKVAKLP
                     KDLLDRRVEITGPVDRKMVINALNSGASCYMADFEDSTTPTWENQLDGQRNLMDAVRR
                     QITFDDRGSGKAYRLNQKTAVLQVRPRGWHLPENHIAIDGRPMSGSLFDFGLYFFHNA
                     KELVARGTGPYFYLPKLESHLEARLWNDVFLFAQDKLGLPKGTIKATVLIETITAAFE
                     MDEILYELRDHSAGLNCGRWDYIFSFIKKFARDPKAAMPDRASVTMTSHFLRSYSLLV
                     IKTCHRRGVHAIGGMASQI"
     gene            complement(992..1495)
                     /locus_tag="VN627_10345"
     CDS             complement(992..1495)
                     /locus_tag="VN627_10345"
                     /inference="COORDINATES: protein motif:HMM:NF009932.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HXD03695.1"
                     /translation="MARIQIPIHPLTRDAFRLYGDVIQVAESRHYTINQGWAERYADI
                     AQLDLLEGEGKPNVGIVRAEPRPIPLKVKLMERHPKSSQLFIPLVPRPFLVLVAAPAD
                     QPRPENLVAFKTMAGQGVNYGRGVWHHPLIALDQSTDFLVVDRHAKDENCDEVSLEHA
                     DLWVGEI"
     gene            complement(1505..2497)
                     /gene="alc"
                     /locus_tag="VN627_10350"
     CDS             complement(1505..2497)
                     /gene="alc"
                     /locus_tag="VN627_10350"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HXD03696.1"
                     /translation="MEQPQHPFGSNLVNLADARLGAEAIFATDDFFAPMERMLNPEPA
                     IFIPGKYDEHGKWMDGWESRRKRTEGHDYCVVKLGLPGSVVGFDIDTSHFTGNFPPAA
                     SIEGCWLDGVPGQGTEWSEIVPATSLKGNSHHFVPVTDTRTWTHLRLNIYPDGGVARL
                     RAYGRVAVDWSQFDRKQPVDLAAVTNGGRAIACSDQHYGSPMQVLYPGRGVNMGDGWE
                     TRRRREPGNDWAIFALGHRGQIRKIDIDTAHFKGNFPDRCSLQAADVTAGTDESLITQ
                     SMFWRTLLPEQKLEMDRPHSFQKEVAELGPITHVRLNIIPDGGVSRLRLWGELV"
BASE COUNT          477 a          861 c          864 g          505 t
ORIGIN      
        1 gatctgcgac gccatgccgc cgatcgcatg gacaccgcgc cgatgacagg tcttgatcac
       61 caagaggctg taggagcgca ggaaatgcga agtcatggtg acgctggcgc ggtccggcat
      121 tgccgccttg gggtcgcggg cgaatttctt gatgaagctg aagatgtagt cccagcgccc
      181 gcaattgagc ccggcggaat gatcgcgcag ctcgtagagg atctcgtcca tctcgaacgc
      241 cgcggtgatg gtctcgatga ggacggtcgc cttgatcgtg cccttgggca ggccgagctt
      301 atcctgcgcg aacaggaaca cgtcgttcca cagccgcgcc tcgagatgcg attccagctt
      361 gggcaggtag aaatagggcc cggtgccccg tgcgaccagt tccttcgcgt tgtgaaagaa
      421 atagaggccg aaatcgaaca ggctgccgga catcggccgc ccgtcgatgg ctatgtgatt
      481 ctccggcaga tgccagccgc gcgggcgcac ctgcaggacg gcggtcttct ggttgaggcg
      541 ataagccttg ccgctgccgc gatcatcaaa ggtgatctgc cttcgcaccg cgtccatcag
      601 gttgcgctgt ccgtcgagct ggttctccca ggtcggcgtg gtcgaatcct cgaaatccgc
      661 catgtagcag gaggcgccgg aattgagggc gttgatcacc atcttgcggt cgaccgggcc
      721 ggtgatctcg acccgccggt ccagcaggtc cttgggcagc ttcgcgacct tccagtcgcc
      781 ggcgcggatc tgcgcggtct ccttcaggaa gtccggcttc tcgcccgcat cgagccgcct
      841 ctgccgctcc ttgcgcgctt cgagcaggcg cagccgctct ggcccgaatt tgcgctcgag
      901 ctccgccagg aagcccaagg cctccggcgt caagatttgt tgaaaagcgg gatcgggaag
      961 cggggcaacc gaaacaccag gcaaagtcac ttcagatttc tccaacccat aaatcggcat
     1021 gctcgaggga aacctcgtcg caattctcat ccttggcatg tcggtccacg accaggaaat
     1081 ccgtactttg gtcgagggcg atcagcgggt gatgccagac gccgcggccg taattcaccc
     1141 cctgccccgc catggtcttg aaagcaacca ggttttccgg ccggggctgg tcagccggcg
     1201 ccgcaaccaa taccaggaat gggcgcggca ccaatggaat gaacagctgg ctgctcttcg
     1261 ggtggcgttc catcagcttg accttcagcg gaatgggcct tggctcggcc cgcacgatcc
     1321 cgacattggg cttgccctcg ccttccagca ggtcgagctg ggcgatgtcc gcatagcgct
     1381 ccgcccagcc ctggttgatg gtgtagtgcc ggctctccgc cacctggatc acatcgccat
     1441 aaagacggaa cgcgtcgcgg gtcagcggat ggatcggtat ctgaatgcgc gccatcggga
     1501 tctcctagac caactcgccc cacaggcgta ggcggctgac cccgccatcg gggatgatgt
     1561 tgagccgcac atgggtgatg ggccccaact ccgccacttc tttctggaag ctgtgcggcc
     1621 ggtccatctc cagcttctgt tcgggcagca aggtgcgcca gaacatggat tgggtgatca
     1681 aggactcgtc cgtgccggcg gtgacgtcgg ccgcctggag cgagcagcgg tcggggaagt
     1741 tgcctttgaa atgcgcggta tcgatgtcga tcttccggat ttgcccgcga tggccgaggg
     1801 cgaagatcgc ccaatcattg cccggctcgc gcctgcgccg cgtctcccac ccatcaccca
     1861 tgttcacgcc gcggccggga tagagcactt gcatgggaga gccgtaatgc tgatcgctgc
     1921 aggcgatggc gcggccgcca ttggtgacgg ccgccagatc gaccggctgc ttgcggtcga
     1981 actgcgacca atcgaccgcg accctgccat aagcgcgcaa tcgcgccacg ccgccatcgg
     2041 gatagatgtt gagccgcagg tgagtccagg tgcgcgtgtc ggtcacgggc acgaagtgat
     2101 gcgaattgcc cttgaggctg gtggcgggca cgatctcgct ccattccgtg ccttggcccg
     2161 gcacgccgtc gagccagcag ccttcgatcg aggcggccgg cgggaaattg ccggtgaaat
     2221 ggctggtgtc gatgtcgaac ccgacgacag agccgggcag gcccagcttc accacgcagt
     2281 aatcgtggcc ctcggtgcgc ttgcggcgcg attcccagcc atccatccac ttgccgtgct
     2341 cgtcgtactt gccggggatg aagatggcgg gctcgggatt cagcatccgc tccatgggcg
     2401 cgaagaaatc gtcggtggca aagatcgcct ccgcccccag ccgcgcgtcg gcgaggttca
     2461 ccaggttgct gccgaacgga tgttgcggct gttccatcat cgcatgttcc tccaaacaac
     2521 atcccgctcc tgtcgcacag cgaacccctt ccccccttgt gggggaaggt tgggatgggg
     2581 ggtgacgccg cacaattgca gacgaagcgt gatgaagggc ttcgcgaggc ctcgcgcgca
     2641 atcgcaacgt ttcggcggtg ttacgcctgc agacgctcgt gcggaggcac cccccatcca
     2701 cccccgg
//
LOCUS       DATNBV010000436         2217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4718686,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000436 DATNBV010000000
VERSION     DATNBV010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4718686"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VN627_10355"
     CDS             <1..688
                     /locus_tag="VN627_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXD03697.1"
                     /translation="SRDIRYRFMFDPDWIHQDMADILGRILRIPVDRKPISILDISAV
                     PLEITNVVVSLICRLVFDFAVRGDRRNVMPILVACDEAHRYIPRDDVPGFEPARLAFG
                     RIAKEGRKYGVSLCLVTQRPSELSETVLSQCSTVIALRMSNSEDQDYVLSRLPDSASG
                     LLSMLPTLGQQEAILVGEGTVHPMRVRFGDLEAQHRPRTAPTSFPQAWDKDEKGRKYL
                     TEVIQRWRGW"
     gene            852..>2217
                     /locus_tag="VN627_10360"
     CDS             852..>2217
                     /locus_tag="VN627_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXD03698.1"
                     /translation="MSRRADGSLSRTTYAALAVRARKLASVLRELGVEAGDRVGTLAM
                     NSDRHMELYYAISGIGAVCNTINPRLSHDDIAYIAGHAEDHVIFCDPRFFPIVAAIAA
                     KVDGLRAVVVLGEAGAMPQAALPAGVALHCYETLMDGADPIGQWPQFDENTASGLCYT
                     SGTTGRPKGVRRAPSTPEQQAKGAAEVGKHWGLVNDPSIVVMMNGPMYHSAPAAYGMQ
                     GARLGLNVVLQPRFEAEDMLRLIDRHRVSHMHIVPTMFVRLLRLPAEVKAKYDLSSLR
                     WITHGAAPCAPAVKRQMIDWWGPVINEYYGATETGIVVWHNAAEALRKPGTVGRVVDG
                     ATMRIVDEQGHDVKQGEVGEIYLRGPLVSDFTYNNDDAKRREVALGDLVTVGDVGYQD
                     PDGYLYLCDRKRDMIITGAVNIYPAEIESVLIQMPGVRDCAVFGIPDEEFGEQICAHI
                     EPVSG"
BASE COUNT          382 a          720 c          735 g          380 t
ORIGIN      
        1 gtcgcgcgac attcgctatc gtttcatgtt cgatcccgac tggatccacc aggacatggc
       61 ggacatattg ggccgcatcc tgcgcatacc cgtggaccga aagccgattt ccatccttga
      121 catttcggcg gtgcctttgg aaatcaccaa tgtcgtggtg tcactgattt gccggctggt
      181 attcgatttc gcggtgcgcg gcgaccgccg caatgtcatg ccgatcctgg tcgcctgcga
      241 cgaggcgcat cgctacattc cccgcgacga tgtgcccggc ttcgagccgg cgcggctggc
      301 gttcggccgc atcgccaagg agggccgcaa atacggcgtg tcgctgtgcc tggtgacgca
      361 aaggccttcg gagctttccg agaccgtgct gtcccaatgc agcaccgtca tcgccttgcg
      421 catgtccaac agcgaagacc aggactatgt gctgagccgg ctgcccgact cggcaagcgg
      481 gcttctcagc atgctgccca ccctgggcca gcaggaagcc atcctggtgg gcgagggcac
      541 cgtgcatccc atgcgggtcc ggttcgggga cctggaggcg cagcaccggc cgcgcaccgc
      601 gcccaccagc ttcccccagg cctgggacaa ggacgaaaag gggcggaaat acctgaccga
      661 ggtcatccag cgctggcgcg gctggtgacg cgcttgttgc gccgcacgcg ttgccagcgt
      721 caatcgcctg tgcgtgaata ggccaggtca tcgcttggga gcagcgcatg caaggcctga
      781 tgcagcgcca tgaattgctg atctcgtcca tcctggaaca tgccgcgcgc catcatggcg
      841 atggcgagct ggtgtcccgc cgcgccgacg gatccctgtc ccgcaccacc tatgccgcgc
      901 tggcggtacg cgcccgcaag ctggcatcgg tgttgcgcga acttggggtc gaagcaggcg
      961 accgggtcgg caccttggcg atgaacagcg accggcacat ggagctctac tatgccatct
     1021 ccggcatcgg cgcggtgtgc aacaccatca atccccgcct gtcgcatgac gacatcgcct
     1081 atatcgcggg ccatgccgaa gatcatgtga tcttctgcga tccgcggttt tttcccattg
     1141 tcgccgccat cgcggcgaaa gtggacggct tgcgcgcggt ggtggtgctg ggcgaggcgg
     1201 gcgccatgcc gcaagcggct ttgccggccg gtgtcgccct gcattgttat gaaaccttga
     1261 tggatggggc cgatccgatc ggacagtggc cgcaattcga cgagaacacc gcgtcgggcc
     1321 tttgctacac ctccggcacc acgggccggc ccaagggcgt gcggcgcgcg ccctcgacgc
     1381 cggagcagca ggccaagggc gcggccgagg tcggcaagca ttggggcctg gtcaacgatc
     1441 cgtcgatcgt cgtgatgatg aacgggccca tgtaccactc ggcgccagcg gcctacggca
     1501 tgcagggcgc gcggctcggg ctgaacgtgg tgctgcagcc gcgcttcgag gccgaggaca
     1561 tgctgcggct gatcgaccgg caccgggtca gccacatgca catcgtgccg accatgttcg
     1621 tgcgcctgct gcggctgccg gccgaggtga aggcgaaata cgacctgtcg agcctgcgct
     1681 ggatcaccca tggcgccgca ccctgcgcgc cggcggtgaa gcggcagatg atcgattggt
     1741 ggggcccggt catcaacgaa tactacggcg ccaccgagac cggcatcgtg gtctggcaca
     1801 atgcggctga ggcgctgcgc aaacccggca ccgtgggccg cgtggtcgat ggtgccacca
     1861 tgcgcatcgt cgacgagcaa ggccacgacg tgaagcaggg cgaggtcggc gaaatctacc
     1921 tgcgcggccc gctggtgtcg gacttcacct acaacaacga cgacgccaag cggcgcgagg
     1981 tcgcgctcgg tgacctcgtg acggtcggcg acgtcggcta ccaggacccc gacggctacc
     2041 tttacctgtg cgaccgcaag cgcgacatga tcatcacggg cgcggtgaac atctatccgg
     2101 ccgagatcga atcggtgcta atccagatgc cgggtgtgcg cgactgcgcg gtgttcggca
     2161 tcccagacga agaatttggc gagcagatct gcgcccatat cgagcccgtc agcggcg
//
LOCUS       DATNBV010000437         3126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_262607,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000437 DATNBV010000000
VERSION     DATNBV010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262607"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..1862
                     /gene="cysN"
                     /locus_tag="VN627_10365"
     CDS             12..1862
                     /gene="cysN"
                     /locus_tag="VN627_10365"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HXD03699.1"
                     /translation="METHQHKTMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLDAL
                     HSDSKKVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFNTEKRKFIVADCPGHEQY
                     TRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLIGIRNLVLAVNKMDLIDYDQA
                     QYDAIVADYAEFAKSIGIEGFTAMPISGFKGDNITTRSENTPWYQGPTLVDHLESVEV
                     NSARDAGKAFRMPVQWVNRPNLDFRGFSGLIATGAVKPGDEVRVLPSGKTSKVTRIVT
                     MDGDLDEAVAGQSVTVCFADEIDCSRGNVISVADNPPEVADQFEATLVWLNDEALHVG
                     RSYWLKLGAQTVSATVQQPKYAVNVNTMEHTAVKTLDLNAIGVAELATDKPVVFEPYA
                     ENRTLGGFILIDKITNATVAAGMLNFSLRRAQNVHWQALDISREAHASLKNQKPAVLW
                     FTGLSGSGKSTIANLVEKKLHRMNRHTFLLDGDNVRHGLNKDLGFTEADRIENIRRVG
                     EVAKLMTDAGLIVITAFISPFRAEREMVRSLIADGEFLEVFIDTPLSVAEERDVKGLY
                     KKAREGQLKNFTGIDSPYEAPVNPEIRVDTTSMTPEAAATLIVDKLLGDA"
     gene            1883..2617
                     /locus_tag="VN627_10370"
     CDS             1883..2617
                     /locus_tag="VN627_10370"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HXD03700.1"
                     /translation="MTDADLAAHLAEVAGKLLLEVRASGMLSLKALGKAGDATANQFL
                     VHALKEQRPDDGLLSEEEKDNAERLAQSRVWIVDPVDGTREYGEERVDWAVHVGLAID
                     GVAAIGAVALPGAGVVLRTDRPGTVPPAPEVLRMVVSRTRPAKEATEVAARIGAELVP
                     MGSAGAKAMAIVRGEADIYLHSGGQYEWDSCAPVAVALAHGLHCSRIDGSPLVYNQAD
                     VYMPDLLICRKEHAQMVLAEVAKVDA"
     gene            complement(2756..>3126)
                     /locus_tag="VN627_10375"
     CDS             complement(2756..>3126)
                     /locus_tag="VN627_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXD03701.1"
                     /translation="RTAFLPILTQAICAARAHGLAILDGVCNEFRDLDLFRAEAEQGL
                     LLGFDGKTLIHPDQVAPCNAVFSPSSEELARAEAIIAAFALPENAGKGVIRVDGKMAE
                     LLHLEQAQRLMAIAEQIAAS"
BASE COUNT          565 a          981 c         1102 g          478 t
ORIGIN      
        1 tcgacaagta tctggaaacc caccagcaca agaccatgct gcggttcatc acctgcgggt
       61 cggtggatga cggcaagtcg accctgatcg ggcggctgct ctatgacagc aagatgatct
      121 tcgaggacca gctcgacgcg ctgcactccg attccaagaa ggtgggcacg caggggcagg
      181 agatcgactt tgccctgctg gtggacggcc ttgccgccga gcgcgagcag gggatcacga
      241 tcgacgtggc ctaccgcttc ttcaacaccg agaagcgcaa gttcatcgtc gccgactgcc
      301 cgggacacga acagtacacc cgcaacatgg tgactggcgc gagcacggcc gaccttgccg
      361 tgatcctgat cgatgcgcgc aagggcgtgc tggtgcagac ccggcggcat tcgtacctgt
      421 gccacctgat cggcatccgc aacctggtgc tggcggtcaa caagatggac ctgatcgact
      481 acgaccaggc ccagtatgac gcgatcgtgg ccgactatgc cgaattcgcg aagtcgatcg
      541 ggatcgaggg cttcaccgcc atgccgatct cgggcttcaa gggcgacaac atcaccaccc
      601 gttcggagaa tacgccgtgg tatcaggggc cgacgctggt cgaccacctg gaatcggttg
      661 aggtcaactc tgcacgcgat gccggcaagg cgtttcgcat gccggtgcag tgggtgaacc
      721 gcccgaacct cgacttccgc ggcttttccg gcctgatcgc gactggcgcg gtgaagccgg
      781 gcgacgaggt gcgggtgctg ccctcgggca agaccagcaa ggtgacgcgg atcgtcacca
      841 tggatggcga tctcgacgag gccgtggccg gccagtcggt caccgtctgc tttgccgacg
      901 agatcgactg ctcgcgcggg aacgtgattt ccgtggcgga caatccgccg gaagtggccg
      961 accagttcga ggcgacactg gtctggctca acgacgaggc gctgcacgtg ggccgcagct
     1021 actggctgaa gctgggcgcg cagaccgttt cggcgacggt gcagcagccc aagtatgcgg
     1081 tcaacgtcaa cacgatggag cacacggcgg tcaagacgct ggacctcaac gccatcggcg
     1141 tggccgagct ggcgaccgac aagccggtgg tgttcgagcc ctatgccgag aaccggacgc
     1201 tgggcgggtt catcctgatc gacaagatca ccaatgccac ggtcgccgcc ggcatgctca
     1261 acttctcgct gcggcgcgcg cagaacgtgc actggcaggc gctcgacatc agccgcgagg
     1321 cccatgcttc gctcaagaac cagaagccgg cggtgctgtg gttcaccggc ctgtcaggtt
     1381 cgggcaagag caccatcgcc aatctcgtcg agaagaagct gcaccggatg aaccggcaca
     1441 ccttcctgct cgacggcgac aacgtgcgcc atggcctcaa caaggacctg ggcttcaccg
     1501 aggccgaccg gatcgagaac atccgccgcg tgggcgaggt ggccaagctg atgaccgacg
     1561 cggggctgat cgtgatcacc gccttcatca gcccgttccg cgccgagcgc gagatggtgc
     1621 gctcgctgat cgcggacggg gaattcctcg aggtgttcat cgacacgccg ctgagcgtgg
     1681 cagaggagcg cgacgtgaag ggcctctaca agaaggcgcg ggaaggccag ctcaagaact
     1741 tcaccggcat cgacagtccc tatgaggcgc cggtgaaccc ggagatccgc gtcgatacca
     1801 ccagcatgac gcccgaagcg gcggccacgc tgatcgtcga caagctgctg ggagacgcgt
     1861 gatgactgtt gatgccagca cgatgaccga cgcggacctg gccgctcacc tcgcggaagt
     1921 cgcgggcaag ctgctgctcg aagtgcgcgc ctcgggcatg ctcagcctca aggcgctggg
     1981 caaggcgggc gacgcgacgg cgaaccagtt cctcgtccat gccctgaagg agcagcggcc
     2041 cgacgacggg ctgctctccg aagaggaaaa ggacaacgcc gagcgcctgg cgcagagccg
     2101 ggtgtggatc gtcgacccgg tcgacgggac gcgcgaatat ggcgaggagc gggtcgactg
     2161 ggcggtccat gtgggcctgg ccatcgacgg cgtggccgcc atcggcgcgg tggcgctgcc
     2221 gggagccggc gtggtgctgc ggacggaccg gccggggact gtcccgccgg cgccggaagt
     2281 cctgcgcatg gtggtcagcc gcacccgccc ggccaaggag gccacggaag tggcggcgcg
     2341 gatcggggcg gagctggtgc cgatgggttc ggccggggcg aaggccatgg cgatcgtgcg
     2401 cggcgaggcg gacatctacc tgcattcggg cgggcagtac gagtgggact cctgcgcgcc
     2461 ggtggccgtg gccctggcgc acgggctgca ctgctcgcgg atcgacggga gcccgctggt
     2521 ctacaaccag gccgacgtct acatgcccga cctgctgatc tgccggaagg aacatgcgca
     2581 gatggtgctg gccgaggtgg ccaaggtcga cgcctgaact gattgatcct ccccgcttgc
     2641 ggggaggggg accgccgaca ggcggtggag ggggcttgcg ggccagagca gcgcttggct
     2701 tagcccccct ccgacagcgc ttcgcgctgc cacctccccg cttgcgggga ggaattcagc
     2761 tggccgcaat ctgctcggcg attgccatca ggcgctgcgc ctgctcgagg tggagcagtt
     2821 cggccatctt gccgtcgacc cggatgacgc ccttgccggc gttttccggg agggcgaagg
     2881 cggcgatgat cgcctcggcg cgggccagtt cctcggatga cggggagaag accgcattgc
     2941 agggagcgac ctgatccggg tggatcaggg tcttgccgtc aaagcccagc aggaggccct
     3001 gctcggcctc ggcccggaag agatccagat cgcggaattc gttgcacacg ccgtcgagga
     3061 tggcgaggcc gtgagcgcgg gcggcgcaga tcgcctgggt gaggatcggc aggaaagccg
     3121 tgcgtt
//
LOCUS       DATNBV010000438         4521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3746169,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000438 DATNBV010000000
VERSION     DATNBV010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3746169"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VN627_10380"
     CDS             <1..292
                     /locus_tag="VN627_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HXD03702.1"
                     /translation="KRFAEAIDARQASGETLDNRLVRIEDAAIVDAELAGTLARITVR
                     FTADIAAVTRDAAGTVVAGSLDDAIEARDLWTFSRDLTAAGPDWLLDETDEA"
     gene            303..1550
                     /locus_tag="VN627_10385"
     CDS             303..1550
                     /locus_tag="VN627_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HXD03703.1"
                     /translation="MTGAGRPSRTGLLAALAAPFLLAACTIIPPGPPTGPGPAPAPTT
                     TPMPPPAVPPTPPPTALTTGIAPGPAPASLPLAQGRASAALRSFAESCRALLIRTDTS
                     GLTRADDWRPACAAAPTWAPADAPAFFARFFETVRVGDGRSYVTGYYEPEIAGTRTQQ
                     PGFTVPVYRLPPDLVRAWPFETPFEQRVGQPPVGRFDADGRFVAYYDRAQIEDGALAG
                     KGLELGWAADPIELFFLEIQGSGRLIAPDGTVLRLGYAGQNGFPYTAVGQLMRDRGLI
                     GSGPGQYSGSMQGMMAYLRAHPEEGRAMMRANRSYIFFREITGDGPIGAMNVPVRSQV
                     SLAADPLMVPLGAPVLLKTDRTETTGLWVAQDTGGAIKGPNRFDSFWGAGPTARKIAG
                     GMSASGTALVFVPRGTLARLSLK"
     gene            1682..2134
                     /locus_tag="VN627_10390"
     CDS             1682..2134
                     /locus_tag="VN627_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HXD03704.1"
                     /translation="MPPRLPPPPKRLKGRIPPPPPAPPRATPPRPLDSHGLDSSWDRK
                     LAKAAIAPDFTLDLHGHTLDSAHARLDHGLTQAISMGARLVLLITGRPRPSDDHAGRG
                     ERRGAIRAKVLDWLAAGPHGPRIAAVRGAHPRHGGAGALYIVLRRGRD"
     gene            complement(2177..3763)
                     /gene="ahpF"
                     /locus_tag="VN627_10395"
     CDS             complement(2177..3763)
                     /gene="ahpF"
                     /locus_tag="VN627_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HXD03705.1"
                     /translation="MLDAATTEQLKQYLGMLREPIALVATLGADARSAETRQLLETIA
                     GLSDKVTASFDGDDKRVPSFSIASVSNPEASVRFAGVPLGHEFTSLVLALLWAGGHPP
                     KVDDAVLQQIRDLPGDYDFEMYFSLSCHNCPDVVQALTLMALTNPRVHAVLIEGGAFK
                     AEVEDRGVMAVPAVFLDGAMWSSGKVTVEEILAKLDTGAADRVAGEIAAKAPYEVLVV
                     GGGPAGAAAAIYTARKGIRTGVAAERFGGQVMDTMGIENFISVPYTEGPKLAGALEQH
                     VKEYEVDVMNLQTARELIPAARRGGYHEIVLANGASLKARTVILSTGARWRNMNVPGE
                     VEYKAKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVSHVTLIEFDAQLRA
                     DAVLQAKLRSLPNVTVIVSALTSEVLGDGAKVTGLTYKDRTTDAIHTVELEGIFVQIG
                     LVPNTEWLKGAVALSPRGEIEIDQRGETSVPGVFAAGDATTVPYKQIVIALGAGATAA
                     LSAFDYLIRLPAEDKVSEAA"
     gene            complement(3969..4511)
                     /gene="ahpC"
                     /locus_tag="VN627_10400"
     CDS             complement(3969..4511)
                     /gene="ahpC"
                     /locus_tag="VN627_10400"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HXD03706.1"
                     /translation="MGSFSATSFKDGKFVDITEKDVLGKWAVFFFYPADFTFVCPTEL
                     EDLADKYAELQAMGVEVFAVSTDTHFSHKAWHDTSPAISKIKYHMVGDQHHTLTNHFN
                     NMREGVGLADRGTFVTDPSGELQLVEVTPEGVGRNAAELVRKIKAAKYWQEHPGQVCP
                     AKWEEGSETLAPSIDLVGKI"
BASE COUNT          673 a         1676 c         1456 g          716 t
ORIGIN      
        1 caagcgcttc gccgaggcga tcgacgcgcg ccaggcctcg ggcgaaacgc tggataaccg
       61 cctcgtcagg atcgaggatg ccgccatcgt cgatgccgaa ctggccggca cgctggcccg
      121 catcaccgtc cgcttcaccg ccgatattgc cgcggtcacc cgcgacgccg cgggcaccgt
      181 cgtcgccggc tcgctcgacg atgcgatcga agcgcgcgac ctgtggacct tcagccgcga
      241 ccttaccgcc gccggccctg actggctgct cgacgagact gacgaagcct gaggacgcag
      301 gggtgacggg ggcagggcgg cccagccgaa cagggttgct ggccgcgctt gccgcgccgt
      361 tcctgcttgc cgcctgcacg attattccgc cggggcctcc aaccggcccc ggcccggctc
      421 ccgcgcccac gacgacgcca atgccgccgc cggccgttcc gccaacgccg ccgcccaccg
      481 cgctcaccac cggcatcgct cccgggcccg cccccgccag cctgccgctt gcccagggcc
      541 gcgcctcggc cgccctgcgc tccttcgccg aatcgtgccg cgccctgctg atccgcaccg
      601 ataccagcgg gctgacccgc gcggatgact ggcgccccgc ctgcgccgcc gcgcccacct
      661 gggcccccgc cgatgccccg gccttcttcg cccgcttctt cgaaaccgtc cgcgtcggcg
      721 acgggcgcag ctatgtcacc ggctattacg agcccgagat cgccggcacc cgcacccagc
      781 agcccggctt caccgttccc gtctatcgcc tgccgcccga tctcgtccgc gcctggccct
      841 tcgaaacccc gttcgaacaa cgcgtcggcc agccgccggt cggccggttc gatgccgatg
      901 gccgcttcgt cgcctactac gaccgcgcgc agatcgagga cggcgccctt gccggcaagg
      961 gccttgagct cggctgggcg gccgatccca ttgaactgtt cttcctagag atccagggct
     1021 ctggccgcct gatcgcgcca gatggcacgg tcctgcgcct cggctatgcc gggcagaacg
     1081 gcttccccta taccgccgtg ggccagctga tgcgcgatcg cggcctgatc ggctccggcc
     1141 ccggccaata ttcgggctcg atgcagggca tgatggccta tctgcgcgcc catccggaag
     1201 agggccgggc gatgatgcgg gccaaccggt cctacatctt ctttcgcgag atcaccggcg
     1261 atggcccgat cggagcgatg aacgtgccgg tccgctccca ggtcagcctc gccgccgatc
     1321 cgctgatggt ccccctgggc gcccccgtcc tcctcaagac cgaccggacg gaaaccaccg
     1381 gcttgtgggt cgcccaggat accggcgggg cgatcaaggg ccccaaccgg tttgacagct
     1441 tctggggcgc cggccccacc gcccgcaaga ttgccggcgg catgagcgca tcgggcaccg
     1501 cgctggtctt cgtcccgcgc ggcacgctcg cccgcctctc cctcaaatga gcaggccccc
     1561 acgcgggctg acccccgaag aagccgaact ctgggccagg gtcgcggcca cggtgaaacc
     1621 catgcacaaa atcctcccca ctcgggggga ggtggcagcg cgcagcgctg acggaggggg
     1681 agtgccgccc aggctgcccc cgccgccaaa gcgcctcaag ggccgcattc ccccgccgcc
     1741 gccagcgccg ccccgcgcaa caccgccgcg gccgctggac agccacggtc tcgattccag
     1801 ctgggaccgc aaactcgcca aggccgccat cgcgccggac ttcacgctgg atctccacgg
     1861 ccataccctc gattccgccc acgcccgcct cgatcacggc ctgacccagg ccatcagcat
     1921 gggcgcgcgc ctcgtcctcc tgatcaccgg tcgcccgcgc ccgtccgacg accacgccgg
     1981 caggggcgaa cgccgcggcg caatccgcgc caaggtcctc gactggctcg ccgccggccc
     2041 ccacggcccc cgcattgccg ccgtccgcgg cgcccacccc aggcacggcg gggcaggggc
     2101 gctctacatc gtgctcaggc gcggccggga ttgaacctgc gtcttctccc ccatcgaggg
     2161 ggagggttcc gcaaactcag gctgcctcgc tcaccttgtc ttccgccggc agccggatca
     2221 ggtaatcgaa ggccgaaagc gccgccgtcg cccccgcgcc cagtgcgatg acgatctgct
     2281 tgtagggcac ggtcgtcgca tcgcccgccg cgaacacgcc ggggaccgaa gtctcgccgc
     2341 gctggtcgat ctcgatctcg ccgcgcgggg aaagcgccac ggcgcccttc agccactcgg
     2401 tattgggcac cagcccgatc tgcacgaaga tgccttccag ctcgacggtg tgaatagcgt
     2461 ccgtcgtccg gtccttgtat gtcaggccgg tcaccttggc gccgtcgccg agtacctcgc
     2521 tggtcagcgc ggagacgatc accgtcacgt tcggcaagga ccgcagcttg gcctggagca
     2581 cggcatcggc ccgcagctgc gcgtcgaact cgatcagcgt cacgtggctg acaatcccgg
     2641 caaggtcgat cgccgcctcg acgccggagt tgcccccgcc gattaccgcc acccgcttgc
     2701 ccttgaacag cgggccgtcg cagtgcgggc aataggccac gcccttggcc ttgtattcca
     2761 cttcgcccgg cacgttcatg ttgcgccacc gcgcgcccgt ggaaaggatc acggtgcgcg
     2821 ccttcaagct cgccccattg gccagcacga tctcatggta gcccccgcgc cgcgctgccg
     2881 ggatcagttc tcgcgcggtc tggaggttca tcacgtcgac ctcgtattcc ttgacgtgct
     2941 gctccagcgc cccggccagc ttcgggcctt cggtgtaggg caccgagatg aagttctcga
     3001 tccccatggt atccatcacc tggccgccga accgctcggc ggcaacgccg gtgcggatgc
     3061 ccttgcgcgc ggtgtagatc gccgccgccg ctcccgccgg accaccgccg acgaccagca
     3121 cctcgtaagg cgctttcgcg gcgatctcgc cggccacgcg gtcagccgcg ccggtatcga
     3181 gcttggcgag gatttcctcg accgtgacct tgcccgatga ccacatcgcg ccatcgagaa
     3241 acactgcggg cacggccatc accccgcggt cctcgacctc ggccttgaac gccccgcctt
     3301 cgatcagcac ggcatggacg cgcggattgg tcagcgccat cagggtcagc gcctgcacca
     3361 cgtcggggca gttgtggcac gagagcgaga aatacatctc gaagtcgtaa tcgccgggca
     3421 ggtcgcggat ctgctggagg acggcgtcgt cgaccttggg cggatggccg ccggcccaca
     3481 gcagggccag gaccagcgaa gtgaattcat ggcccagcgg aacgccggcg aagcgcacgg
     3541 aggcttcggg gttcgagacg ctggcgatgg agaaggaggg gacgcgcttg tcgtcgccat
     3601 cgaagctggc ggtgaccttg tcggacagcc cggcgatcgt ctccagcaac tggcgggttt
     3661 cggcggagcg ggcatcagcg cccagcgtgg caaccagcgc gatcggttcg cgcagcatgc
     3721 caaggtattg cttcagctgt tcggtggtgg cggcgtcgag catgggaggg aatccttgtc
     3781 tgatcctccc cactgtgtgg ggaggtggca gcccgcaagg gctgacggag ggggttatcg
     3841 gctcaagctg atcccggatg ggtgcagctg cgccgcccct ccacctgttc ctgcgaggtg
     3901 gtgggaggag gcgaaccccc tccaccccct gcggcggtcc ccctccccgc aagcggggag
     3961 gattaggatc agatcttgcc gacgaggtcg atcgacgggg cgagggtttc gctgccctct
     4021 tcccacttgg ccgggcagac ctggccgggg tgttcctgcc agtacttggc ggccttgatc
     4081 ttgcggacga gttcggcggc gttgcggccg acgccttccg gggtgacttc gaccagctgc
     4141 agctcgcccg agggatcggt gacgaaggtg ccgcggtcag ccaggcccac gccttcgcgc
     4201 atgttgttga agtggttggt cagcgtgtga tgctggtcgc ctaccatgtg gtacttgatc
     4261 ttgctgatcg ccggcgaggt atcgtgccag gccttgtggc tgaagtgcgt gtcggtcgac
     4321 acggcgaaga cttccacgcc catcgcctgg agctcggcat acttgtcggc gaggtcttcc
     4381 agctcggtcg ggcagacgaa ggtgaaatcg gccgggtaga agaagaacac cgcccacttg
     4441 ccgagcacgt ccttctcggt gatgtcgacg aacttgccgt ccttgaacga ggtggccgag
     4501 aacgagccca gcttgctgcc a
//
LOCUS       DATNBV010000439         2616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3452013,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000439 DATNBV010000000
VERSION     DATNBV010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3452013"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..465)
                     /locus_tag="VN627_10405"
     CDS             complement(88..465)
                     /locus_tag="VN627_10405"
                     /EC_number="5.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004769 - steroid delta-isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0008202 - steroid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="steroid Delta-isomerase"
                     /protein_id="HXD03707.1"
                     /translation="MPDPTAMEAAVHEYVAAFAAGSADRVAALYAADATVEDPIGSPM
                     HHGREAIRAFYSESMKTGAKLKLEGPIRVAGDYAAFPFSVNLHWVGSDKRIDVIDTFR
                     FNEANEVVEMRAFWGPANMHGFV"
     gene            complement(471..944)
                     /locus_tag="VN627_10410"
     CDS             complement(471..944)
                     /locus_tag="VN627_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXD03708.1"
                     /translation="MPVVFEKPADLIGKGGTILPPTEWLTIDQERVNGFAEVTGDDQW
                     IHVDVDRAKEGPFGGTIAHGYLTLSLVNYFLPQLIEVRGFAHAVNVGADRLRFLSPVK
                     VGSRIRGSGEVISAEEVKGGIQSVVRVTIQIEGQDGQPTDRPACVLDTISRYFPE"
     gene            complement(947..1732)
                     /locus_tag="VN627_10415"
     CDS             complement(947..1732)
                     /locus_tag="VN627_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD03709.1"
                     /translation="MTVPVPAYPTPLGMLKGKTVVITAAAGTGIGFAVAKRAAEEGAR
                     LLISDFHERRLGEAADRIAAEVGCERPATCVCDVTGEEAVQGLRDASLAALGKVDVLI
                     NNAGLGGEVDVVDMTDDQWGRVLDVTLTSLFRMTRAFLPSMYANKSGVMVNNASVLGW
                     RAQRGQAHYAAAKAGVMAFTRCTALEAADHGVRINAVAPSLAMHPFLAKVTTEEKLAE
                     LVKTEAYGRPAEVWEVANVMLFLASDLSSYMTGEIVAVSSQRA"
     gene            complement(1729..>2616)
                     /locus_tag="VN627_10420"
     CDS             complement(1729..>2616)
                     /locus_tag="VN627_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD03710.1"
                     /translation="LAGPTILAFGSDEQKARYLPGIAAGRHIWCQGYSEPNAGSDLAN
                     VRTKARIDGGDWVVNGQKVWTSLAHYSDMIFVVARSEDGSQGPKGLSFLLMEMDQPGI
                     EVRPIRQINGEAEFNETFLTDARCPADAMLGAAGEGWKVAMALLAFERGVSTLGQQMQ
                     FRNELDAVIAAARANGKAGDAVIRQRIAQAEIGLRLMRYGALRMLSNADAGAIDGAAL
                     TYKIQWATWRRNLGELAMDVLGQAGEIAEGDHYGWSMLPNLYLFSRSDTIYGGTNQIQ
                     RNLIAERGLGLPREPRGQA"
BASE COUNT          426 a          862 c          870 g          458 t
ORIGIN      
        1 ggcgccgcga taggctttgc cgacccctgt gcgggcggtg gaaacgatag ctgcatcacg
       61 catgggaagt cctctcgaac attaaggtca gacaaagccg tgcatgttgg cggggcccca
      121 gaaggcccgc atttcgacga cttcgttggc ttcattaaag cggaaggtat cgatcacgtc
      181 gatccgtttg tccgatccta cccaatgcag gttcaccgag aacgggaacg ccgcatagtc
      241 acctgcgacc cgaatcggac cttcaagctt cagcttggcg ccggtcttca tcgattcgga
      301 atagaacgcg cggatcgcct cgcgcccgtg atgcatcggg ctgccgatcg ggtcttcgac
      361 cgtggcatcg gcggcataaa gcgcggccac gcgatcagcg cttcccgctg cgaaggcggc
      421 gacatattcg tgcactgcgg cttccattgc cgtgggatcg ggcatctcgt tcactccggg
      481 aaatagcggc tgatcgtgtc gagcacgcag gcgggccgat cggtcggttg tccatcctgc
      541 ccttcgatct ggatcgtgac gcgtacgacc gactggatac cgcctttcac ttcctcggcg
      601 gagatgacct cgcccgaccc gcgaatgcgg ctgccgacct tcaccggcga gaggaagcgc
      661 aggcggtccg ccccgacgtt gacggcatgc gcaaaaccgc ggacctcgat cagctgcggc
      721 aggaaatagt tgaccaggct cagcgtgagg tagccgtggg cgatggtgcc gccgaacgga
      781 ccttctttcg cgcggtccac atcgacatgg atccactggt catcgccggt cacctcggca
      841 aagccgttga cccgctcctg gtcgatcgtc agccattcgg tcggcgggag gatcgtcccc
      901 cccttgccga tcagatcagc cggcttttcg aagacgaccg gcacgatcag gccctctggc
      961 tcgagacggc gacgatctcg ccggtcatgt aggaggacag gtcgctcgcg aggaacagca
     1021 tgacgttggc cacttcccac acttcggcgg ggcggccgta ggcctcggtc ttgaccagtt
     1081 cggcgagttt ctcctcggtc gtcaccttgg cgaggaacgg gtgcatggcg aggctcggcg
     1141 ccacggcatt gatccgcacg ccgtgatcgg ccgcttccag cgcggtgcag cgggtgaagg
     1201 ccatcacccc ggccttggcc gcggcatagt gggcctggcc ccgctgcgca cgccagccca
     1261 gcaccgaggc gttgttgacc attacgccgg acttgttcgc atacatggag ggcagaaagg
     1321 cccgggtcat gcggaacagg ctggtgaggg tcacgtccag cacgcggccc cactggtcgt
     1381 cggtcatgtc gaccacgtcg acctcgccgc cgaggccggc attgttgatc agcacgtcaa
     1441 ccttgcccag cgccgcgagc gaggcatcgc gcaggccctg gacggcctcc tcgccggtca
     1501 cgtcgcagac gcaggtggcg gggcgttcgc agccgacttc ggcggcgatg cgatcggccg
     1561 cttcgccgag gcggcgctca tgaaaatcgc tgatcaggag acgcgcgcct tcctcggcgg
     1621 cgcgcttggc gacggcgaag ccgatgccgg ttccggcggc agccgttatc accaccgtct
     1681 tgcccttgag catgccgagc ggcgtgggat aggcgggtac gggaacagtc atgcttggcc
     1741 tcttggctcg cggggaagac ccagaccgcg ctcggcgatc aggttgcgct ggatctggtt
     1801 ggtgccgccg tagatcgtgt cggagcggct gaagagatag aggttgggca gcatcgacca
     1861 gccgtagtga tcgccctcgg cgatctcccc ggcctggccg agaacgtcca tcgccagctc
     1921 gccgagattg cggcgccagg tggcccactg gatcttgtag gtaagcgcgg cgccatcgat
     1981 cgcgccggca tccgcgttgg agagcatccg cagcgcgccg tagcgcatga gccggaggcc
     2041 gatttcggcc tgggcgatgc gctggcggat caccgcgtcg cccgccttgc cgttggccct
     2101 ggcagcggcg atcacggcgt cgagttcgtt gcggaactgc atctgctggc cgagcgtcga
     2161 gacgccgcgc tcgaaggcca gcagcgccat ggccaccttc cagccttcgc ccgccgcgcc
     2221 cagcatggca tcggccgggc agcgggcgtc ggtcaggaag gtctcgttga actcggcctc
     2281 gccgttgatc tggcggatcg ggcgcacctc gatccccggc tggtccatct ccatcagcag
     2341 gaacgacagg cccttgggcc cctgcgatcc gtcctcgctg cgggcgacga cgaagatcat
     2401 gtcggaatag tgggcgaggc tggtccagac cttctggccg ttcacgaccc agtcgccgcc
     2461 atcgatccga gccttggtcc tgacgttggc aagatccgag ccggcattcg gctcggagta
     2521 gccctggcac cagatgtgcc tgccagcggc gatgccgggc aggtaccgag ccttctgctc
     2581 gtccgacccg aaggccagga tcgtcggccc ggccag
//
LOCUS       DATNBV010000440         2086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3068755,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000440 DATNBV010000000
VERSION     DATNBV010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3068755"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..582)
                     /locus_tag="VN627_10425"
     CDS             complement(25..582)
                     /locus_tag="VN627_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HXD03711.1"
                     /translation="MTVHRPDVTILPYLGKYPRIHDSAFIAPGCRIIGDVEIGAEASI
                     WYNCVVRGDVNFIRIGDRSNIQDLSMLHVTHKKNAADPGAPLVIGNDVTVGHSVTLHG
                     CTIEDGAFIGMQAMIMDKAVVGKGALVGARALVTEGTVIPPGTLWVGAPAKYKRDLTA
                     DEVAWLARSAGNYVRYSREYLEDTP"
     gene            complement(585..1607)
                     /gene="hemB"
                     /locus_tag="VN627_10430"
     CDS             complement(585..1607)
                     /gene="hemB"
                     /locus_tag="VN627_10430"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HXD03712.1"
                     /translation="MSQANAFGAYPALRLRRTRASAWSRALHREIAVTPADLIWPLFV
                     TDGSDDLGVVEEPIGSLPGVSRWCVKGVAQRAKEAAALGIPCLALFPNTQPGRRDPMG
                     KEALNPDNLMCRATRAIKDAVGDAVGVLTDVALDPYTSHGQDGLIDEAGYVLNDETAE
                     VLVAQSLNQAAAGADIIAPSDMMDGRVGLIRAALEGAGHRNVQIMSYAAKYASAFYGP
                     FRDAVGSSGLLKGDKKTYQMDPANAEEVLREVALDLAEGADTVMVKPGLPYLDVAFRV
                     KQAFQVPVFAYQVSGEYAMIEHAVAAGAADRDAMVLETLTAFKRAGCSGVLTYHAAHA
                     ARLLGA"
     gene            complement(1645..>2086)
                     /locus_tag="VN627_10435"
     CDS             complement(1645..>2086)
                     /locus_tag="VN627_10435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXD03713.1"
                     /translation="MHAGVDFGAAWGSPIVASAAGVVSYAGYHGGHGNYVRLEHGGGL
                     GTGYGHMSRIAVAPGERVSAGEVIGYVGSTGLSTGPHLHYEMYRDGRTVNPLSQSFAS
                     VTVTRTVDAGQLAAFRTRLAQLRALRPGPALAALGQAVHRLALR"
BASE COUNT          336 a          683 c          733 g          334 t
ORIGIN      
        1 gcgggacccc ggtgactacc tgatttacgg cgtgtcctcg aggtactccc gcgaatacct
       61 gacgtagttc ccggcggagc gggcgagcca ggccacctcg tctgcggtga ggtcacgctt
      121 gtacttggcc ggggctccga cccagagggt tcccggaggg atcacggtcc cttcggttac
      181 cagggcgcgg gcgcctacca gtgcgccctt gccgaccacg gccttatcca tgatcatcgc
      241 ctgcatcccg ataaaggcgc cgtcctcgat ggtgcagccg tgcagggtga cgctgtggcc
      301 gacagtcacg tcgttgccga tcacgagcgg cgcccccgga tcggcggcat ttttcttgtg
      361 ggtcacgtgc agcatggaaa gatcctggat gttactccgg tcgccgatgc ggatgaagtt
      421 tacatcgccc cggaccacgc agttgtacca gatgctggcc tcggccccga tctcgacatc
      481 gccgatgatc cggcaaccgg gggcaatgaa ggcgctgtcg tggatgcgcg ggtacttgcc
      541 caggtagggc aggatggtga catcaggccg gtgcacggtc atcctcacgc tccgagcagg
      601 cgggccgcgt gggccgcgtg ataggtcagc acgcctgagc agccggcgcg cttgaaggcg
      661 gtcaacgttt ccagcaccat ggcatcgcga tcggccgccc cggccgcgac ggcatgctcg
      721 atcatcgcgt attcgcccga gacctggtag gcaaagaccg gcacctggaa cgcctgcttc
      781 acccggaagg ccacatcgag atagggcagg ccgggcttga ccatgaccgt atcagcgccc
      841 tcggcgagat cgagcgccac ctcgcgcagg acttcctcgg cgttggccgg gtccatctgg
      901 taggttttct tgtctccctt gagcaggccc gacgagccga ccgcatcgcg aaacgggcca
      961 tagaacgccg aggcatattt ggcggcatag ctcatgatct ggacgttgcg gtgcccggcg
     1021 ccttcaagcg cggcgcggat caggccgacg cggccgtcca tcatgtcgga cggagcgatg
     1081 atgtcggccc cggcagcagc ctggttgagg ctttgcgcga cgaggacctc ggcggtctca
     1141 tcattgagga catagccggc ctcgtcgatc aggccgtcct gcccgtggct ggtataggga
     1201 tcgagcgcca cgtcggtcag cacgccgacg gcatcgccga ccgcatcctt gatcgcgcga
     1261 gtggcgcggc acatcaggtt gtccggattg agcgcttcct tgcccatggg gtcacgccgt
     1321 ccgggctggg tgttggggaa gagcgccagg caggggatgc cgagcgccgc cgcctccttg
     1381 gcgcgctgcg caacgccctt gacgcaccag cgcgagacgc cgggaagcga gccgatcggt
     1441 tcttccacga ccccaaggtc atcggagcca tcggtcacga acagcggcca gatcaggtcg
     1501 gccggggtga cggcaatttc gcggtggagg gcgcggctcc aggcagaggc gcgggtgcgg
     1561 cgcaggcgca gggcgggata ggcgccgaag gcattggctt ggctcatgcc gcgcgtcctg
     1621 ccggaaacgg acgcgcggtt caacttatct gagggcgagc cggtgaaccg cctggcccaa
     1681 ggcggccagc gccggcccgg ggcggagcgc gcgcagctgc gcgagcctgg tgcggaacgc
     1741 agcgagctgg ccggcatcga ccgtgcgggt gacggtcacg ctggcgaagc tctggctgag
     1801 cgggttcacc gtgcgcccat cgcggtacat ctcgtaatgc aggtgcgggc cggtcgagag
     1861 gccggtcgat ccgacgtagc cgatgacctc gcccgcgctg acccgctcgc cgggggcgac
     1921 ggcgatgcgg ctcatgtgcc catagccggt gccgagcccg ccaccgtgct cgagacgaac
     1981 gtaattgccg tggccgccat ggtagccggc ataggacacg acccccgccg ccgaggcgac
     2041 gatcggcgat ccccaggccg cgccgaaatc gacgccggcg tgcatg
//
LOCUS       DATNBV010000441         2034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_885684,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000441 DATNBV010000000
VERSION     DATNBV010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885684"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VN627_10440"
     CDS             complement(<1..248)
                     /locus_tag="VN627_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03714.1"
                     /translation="MARSRFKIILINSDGAAYVVMSARSAISASPLVIPAEQAITLLP
                     AMPLPLFRHPGESRGQGPLASCSPCGPWIPAFAGMTAEG"
     gene            384..869
                     /locus_tag="VN627_10445"
     CDS             384..869
                     /locus_tag="VN627_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HXD03715.1"
                     /translation="MPSFDVVSKTDLNEVDNALHNIEREIATRFDFKNSKSKIERQEA
                     VLTLNADDQPKLKQMHELLKAHMVRRKIDPSALEFKDPERASGDMVRQEIVIKQGIDR
                     ELAKQLVKAIKDAKLKVQAAIQGDELRISGKKRDDLQEAIALIRTLKVEQPLQFVNFR
                     D"
     gene            912..>2034
                     /locus_tag="VN627_10450"
     CDS             912..>2034
                     /locus_tag="VN627_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXD03716.1"
                     /translation="MPLRISDFPFHAAGLFPDRDAAVLGERRLSYRALTEEIERCARA
                     LVAYGIVPGDRIAMLSTPRPEYLVVFLASARIGAIWLGLNPVHRLAEHRQILEDSQPR
                     LLFGFPQLRDRDHRQDLQELTAALGCIEGLVVLGEGEGLGIGYEEFLERGRSVEAEAY
                     AAAVAAVQPDDVALIVYTSGSTGGSKGAMITHRNLVHCATVQHRLFPVDPLIVLCNLP
                     VSHIASSSDIVSHALIGGGTIVFQERFDPAEALALIDGERVSCLLQIPTMLQRILAVP
                     DRRTRDTASLKVLFFEGAPMPADQIPDLQEMARTVVTAWGLTESSCSVTYTAAGDGLA
                     VLAETVGRAAPGYEVLILDAEGRPAADGEAGEVLVRGPCV"
BASE COUNT          356 a          675 c          655 g          348 t
ORIGIN      
        1 ccctctgccg tcatcccggc gaaagccggg atccaggggc cacagggcga gcagcttgcc
       61 aatggcccct gaccccggct ttcgccgggg tgacggaata gagggagcgg catagccggc
      121 aacagcgtta ttgcctgctc cgccggaatg acgaggggag aagcggagat ggccgatctc
      181 gccgacatga cgacataggc agcgccgtcg ctattgatca ggatgatctt gaaccgggac
      241 cgggccatgg ggcggggact ttgcccccgc caactcctgc cgcaacagcc gtctcagcgc
      301 ttcccaagcc cgtcccgaga gcgccccggc ggggaaaatc atgctatgac agggctggtc
      361 cgtccttgcg aagggtctat cccatgccca gtttcgacgt cgtttccaag accgacctga
      421 acgaggtcga caatgcgctg cacaacatcg agcgcgagat cgcgacccgt ttcgacttca
      481 agaactccaa gagcaagatc gagcggcagg aggcggtcct caccctcaat gccgatgatc
      541 agcccaagct gaagcagatg catgagctgc tgaaggccca tatggtgcgc cggaagatcg
      601 atccctcggc cctcgagttc aaggatcccg agcgcgcctc gggcgacatg gtccgccagg
      661 agatcgtgat caagcagggc atcgaccggg agctggccaa gcagctggtc aaggcgatca
      721 aggacgccaa gctcaaggtc caggccgcga tccagggcga tgagctgcgc atcagcggca
      781 agaaacgcga cgatctgcag gaggccatcg ccctcatccg caccctcaag gtcgagcagc
      841 cgctgcaatt cgtgaacttc cgcgattagc gattccgctt aggccgcgac gagtccgcca
      901 tggcaccggc catgccgctt cgcatcagcg attttccgtt ccatgccgcg gggttgtttc
      961 ccgatcgcga cgccgcggtc ctcggcgagc ggcggctcag ctaccgggcg ctgacggaag
     1021 agatagagcg ctgcgcccgg gccctggtcg cttatggaat cgtgccgggc gaccgcatcg
     1081 ccatgctgtc gaccccgcgg ccggaatatc tggtcgtctt cctggcgagc gccaggatcg
     1141 gcgccatatg gctcggtctc aaccccgtcc atcgtctggc cgagcaccgc cagatcctgg
     1201 aggacagcca gcctcgcctg ctcttcggct ttccccagct gcgcgatcgg gaccatcgcc
     1261 aggatttgca ggagctgacg gcggcgcttg gctgcatcga agggctcgtg gtcctggggg
     1321 agggcgaagg tctcggtatc ggctatgagg aattcctcga gcgcggcaga tcggttgagg
     1381 ccgaggccta tgcggccgcc gtcgcggcgg tgcagcccga tgacgtggcc ctaatcgttt
     1441 atacctcggg ctccacgggc ggctccaagg gcgcgatgat cacccatcgc aatctcgtgc
     1501 attgcgcgac cgtccagcat cggctgtttc ccgtcgaccc gctcatcgtc ctctgcaatc
     1561 ttccggtcag ccatatcgcg tcgagcagcg atatcgtctc ccatgcgctc atcggcggcg
     1621 ggaccatcgt cttccaggag cgcttcgatc cggccgaggc cctggcgctg atcgatgggg
     1681 agcgcgtctc ctgcctgctg cagatcccga cgatgctgca gcgcatcttg gccgtgcccg
     1741 atcgccgcac gcgcgataca gccagcctca aggttctatt cttcgaaggc gcccccatgc
     1801 ccgcggatca gattcccgat ctccaggaaa tggcaaggac ggtcgtcacg gcctgggggc
     1861 tgacggaatc ttcctgctcc gtgacctata ccgccgccgg cgacggtctc gccgtgctcg
     1921 ccgagaccgt gggccgggcg gccccaggct atgaggtgct gatcctcgac gccgagggca
     1981 ggcccgccgc cgatggcgag gcgggcgagg tgctggtgcg cgggccttgc gtca
//
LOCUS       DATNBV010000442         6461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3453204,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000442 DATNBV010000000
VERSION     DATNBV010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3453204"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /gene="mraY"
                     /locus_tag="VN627_10455"
     CDS             <1..115
                     /gene="mraY"
                     /locus_tag="VN627_10455"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413324.1"
                     /note="First step of the lipid cycle reactions in the
                     biosynthesis of the cell wall peptidoglycan; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXD03717.1"
                     /translation="IHHHFEKLGWAESTVVIRFWIVSIILAIIGLSTLKLR"
     gene            126..1481
                     /gene="murD"
                     /locus_tag="VN627_10460"
     CDS             126..1481
                     /gene="murD"
                     /locus_tag="VN627_10460"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXD03718.1"
                     /translation="MITSATFAGKRYAVLGLARTGLAVVRTLLASGAEVIAWDSREEP
                     RAAVAAAFGARVTLADPTTADLTGCTGVIVSPGVPLNRHPISEAAARYGVPVIGDIEL
                     FAMARPVLPPHRVIGITGTNGKSTTTALVHHLLTVNGIPSRMGGNIGLPVLGQEPLQP
                     SDHGTGVYVLELSSYQIDLTQTLACDAAALLNLTPDHLDRYGTFAAYAASKARLFAMQ
                     RADQPAVFGINDADTLAIAAREELRRGPGLIHKVHGAEIAAWQGEWPSLQGPHNLQNA
                     AVAVALCAAMGLTADQCRAGLASYKGLPHRMERVADWNGVLFINDSKATNPASAAPAL
                     AAFPPVAGHPRIHWIVGGLPKGDDLDECAPFFGNVAEAYTIGDAGPRFAELLEPHLPV
                     HRSEMMCEAIREALAAAKPGEVVLLSPACASFDQFRDYEARGDSFRQIVGALLAGEDC
                     A"
     gene            1502..2773
                     /locus_tag="VN627_10465"
     CDS             1502..2773
                     /locus_tag="VN627_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HXD03719.1"
                     /translation="MAEAQTPFVPRASVVPQGLRKRRNRGHELFVWWREIDRVVLALV
                     LALMAIGTVAVAAASPASARRLSTAAVRLDDLHFFVLHVRWQLLGLVAMVGASMLPKE
                     QARRAGIVLGFAMIFALMLVPVIGTNVNGAKRWLNLGISLQPSEFLKPAYAIAMAWVL
                     SWKLRDPNLPVLWLASGMMALVAGLLMLQPDFGSTVLFVGVWFVLVLLSGLPVKQIAV
                     LGAGGIVAVVATYFLYDNARHRIDNFLGGGTAYDQVDLASRTITAGGWTGSGLWLGTR
                     KLSLPEAHTDYIFSVIGEEFGLLVCGLIVVLYLALVTRVLLRLIDEDDLFTVLAATGL
                     TAQLGGQAFINILVNLQLFPSKGMTLPLISYGGSSTVALCLGVGFLLAITRRNPYLQR
                     HRVTLREVGRGPQVPSAGLKTGLPKAGVLKK"
     gene            2770..3954
                     /gene="murG"
                     /locus_tag="VN627_10470"
     CDS             2770..3954
                     /gene="murG"
                     /locus_tag="VN627_10470"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HXD03720.1"
                     /translation="MSGVSRHYVLAAGGTGGHLIPAFALAAELDRRGHHVALVTDERG
                     AAIPGKPDFLPAHVIPAGRIEGMNPLGWLKGLRAILEGRRMALRLFDSFAPSAVVGFG
                     GYPALPAVLAATSAKIPSIVHEQNAVLGRVNRFLAKRVDAIATAYAEVDRLDSKLLGK
                     VHLVGNPVRAEVLTLRDQPYPEFTEDGLLRVLVTGGSQGARVLSEVVPDGLSMLQPAL
                     RGRLQVIQQCRAEDVDAVRQRYAGHGIPAELATYFEDMAERLADAHLFIGRAGASTIA
                     ELTAVGRPAILVPLPIATDDHQAANTREIVAAGGARAIRQERFTAVELAKQIQAMAQH
                     PHSLANAAHAAWNCGYPKAAEDLADLVESFGAAPLMDVIRVGGDAPVASGNEAFARDN
                     AA"
     gene            3951..5363
                     /gene="murC"
                     /locus_tag="VN627_10475"
     CDS             3951..5363
                     /gene="murC"
                     /locus_tag="VN627_10475"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HXD03721.1"
                     /translation="MKGVPTDIGTIHFVGIGGIGMSGIAEVMHNLGYKVQGSDIAEGY
                     VVEGLRQRGIKVMIGHKAENLGEAAVVVTSTAVKRDNPEVVAALEHRVPVVRRAEMLA
                     ELMRLKNTVAVAGTHGKTTTTSMIAALLDAGGIDPTVINGGIINSYGSNARLGASDWM
                     VVEADESDGSFLRLDGTLAVVTNIDPEHLDHYGSFDRVKECFVEFIENVPFYGAAMLC
                     IDHPEVQAIIPKVRDRRVITYGFSAQADVRGEGVTPVPGGNRFTAVLRARDGSFRRIE
                     DIVLPMPGRHNVQNALAAVAVAVEMGCSDDCIRGGFAKFGGVKRRFTKVGEIDGVTII
                     DDYGHHPVEIRAVLAAAREGVKGRVIAVVQPHRFTRLRDHLDDFAGAFNDADVVYAAP
                     VYPAGEQPIDGVDSAALVAGAKARGHRSAHLIAGPAELARDLAATVRPGDMVVCLGAG
                     DITRWAAGLAGAIAAEKAPA"
     gene            5360..6256
                     /gene="murB"
                     /locus_tag="VN627_10480"
     CDS             5360..6256
                     /gene="murB"
                     /locus_tag="VN627_10480"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HXD03722.1"
                     /translation="MTTTLALPPVRGKLTAQAPLAPLVWFKAGGAAQFLFEPKDINDL
                     QEFLAALDPAVPVMALGLGSNLIVRDGGVPGVVIRLGKPFARVDALDQLTLSCGAGAS
                     GILVSSTARDAGVAGLEFLRSIPGTVGGFVRMNGGAYGSEVKDILVDCDVVLRSGQLV
                     TLPVADLRYTYRHSELPEGAIVVAAHFRGRPGEPAAIQAEMDRISASREASQPLRSKT
                     GGSTFKNPPGEKAWELVDRAGCRGLQLGGAQVSEKHTNFLINTGEATSAEIEALGEEV
                     RRRVKANSGVELEWEIQRVGKP"
     gene            6253..>6461
                     /locus_tag="VN627_10485"
     CDS             6253..>6461
                     /locus_tag="VN627_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXD03723.1"
                     /translation="MSLPKLHVAVLMGGWSSERPVSLMSGEGVAKALEARGHKVTRID
                     MGRDVAQRLAEAAPDVVFNALHGAPG"
BASE COUNT          963 a         2406 c         2047 g         1045 t
ORIGIN      
        1 gatccaccac cacttcgaaa agctcggctg ggcggaatcg accgtggtga tccgcttctg
       61 gatcgtctcg atcattctcg ccatcatcgg cctgtcgacc ctcaagctgc gatgatcgtc
      121 tccccatgat cacttcagcc actttcgccg gcaagcgcta tgccgtcctc ggcctcgccc
      181 gcaccgggct ggccgtggtg cgcacgctgc tggccagcgg ggccgaagtc atcgcgtggg
      241 acagccggga ggaaccccgc gccgccgtcg ccgccgcctt cggcgcgcgg gtcacccttg
      301 ccgatccgac caccgccgat ctcaccggct gcaccggcgt gatcgtctcc cccggcgtcc
      361 cgctcaaccg ccacccgatc agcgaggccg ccgcccgcta tggggtgccc gtgatcggcg
      421 atatcgaact gttcgccatg gcccggcccg tcctgccgcc ccaccgggtg atcggcatca
      481 ccgggacgaa tggcaagtcg acgacgaccg cgctcgtcca ccacctcctt acggtcaacg
      541 gcatcccgag ccggatgggc ggcaacatcg gcctgcccgt gctcggccag gaaccgctcc
      601 agcccagcga ccacggcacc ggggtctacg tactggagct gtccagctac cagatcgacc
      661 tcacgcagac cctcgcctgc gacgccgcgg ccctgctcaa cctcaccccc gatcatctcg
      721 atcgctacgg caccttcgcc gcctatgcgg ccagcaaggc ccgcctcttt gccatgcagc
      781 gggccgatca gcccgccgtc ttcgggatca acgatgccga taccctcgcc atcgcggccc
      841 gcgaagaact gcgccgaggc cccggcctga tccacaaggt ccatggcgcc gagatcgccg
      901 cctggcaggg cgaatggccc tcgctccagg gcccgcacaa tctccagaac gccgccgtcg
      961 ccgttgccct gtgcgccgcc atgggcctca ccgccgatca gtgccgcgcc ggcctggcca
     1021 gctacaaggg cctgccccac cggatggagc gcgtggctga ctggaacggc gtgctcttca
     1081 tcaacgacag caaggccacc aacccggcct cggccgcccc cgcgctcgcc gccttcccgc
     1141 ccgtcgcggg gcacccccgg attcactgga tcgtcggcgg cctgcccaag ggcgacgatc
     1201 tcgatgaatg cgcccccttc ttcggcaatg tggccgaggc ctacaccatc ggcgatgccg
     1261 gcccccgctt tgccgagctg ctcgaaccgc acctgccggt ccaccgcagc gaaatgatgt
     1321 gcgaggcgat ccgcgaggcc ctggccgccg cgaagcccgg cgaagtcgtc ctgctctcgc
     1381 ccgcctgcgc cagcttcgat cagttccgcg actatgaggc gcgcggcgat tcgtttcgcc
     1441 agattgtcgg cgccctgctc gccggggagg actgcgcatg agtgctccga tcgccaagag
     1501 catggccgag gcgcaaaccc ccttcgtgcc gcgcgccagc gtggtcccgc agggcctgcg
     1561 caagcggcgc aaccgcgggc atgaactgtt cgtctggtgg cgcgaaatcg atcgcgtggt
     1621 gctcgccctg gtcctggccc tgatggccat cggcaccgtg gccgtggccg ccgcctcgcc
     1681 cgccagcgcc cggcgcctgt ccaccgccgc cgtgcggctg gacgatctgc acttcttcgt
     1741 cctccacgtc cgctggcagc tgctcggcct ggtcgccatg gtcggcgcct cgatgctgcc
     1801 caaggagcag gcccggcggg ccggaatcgt gctcggcttc gcgatgatct tcgcgctgat
     1861 gctcgtgccg gtgatcggca ccaacgtgaa cggcgccaag cgctggctga atctcggcat
     1921 ttcgctccag ccgtccgaat tcctcaagcc cgcctatgcc atcgccatgg cctgggtgct
     1981 gtcgtggaag ctgcgcgatc cgaacctgcc ggtgctgtgg ctggcctcgg gcatgatggc
     2041 cctggtcgcc gggctcctca tgctccagcc cgatttcggg tcgaccgtgc tgttcgtcgg
     2101 cgtctggttc gtgctggtcc tgctctcggg gctcccggtc aagcagatcg ccgtgctcgg
     2161 cgcgggcggg atcgtcgccg tggtggcgac ctatttcctc tatgacaacg cccgtcaccg
     2221 catcgacaac ttcctcggcg gcggcacggc ctacgatcag gtggatctcg ccagccgcac
     2281 gatcacggcc ggcggatgga ccggttcggg cctgtggctc ggcacccgca agctgtcgct
     2341 gcccgaagcc cataccgact atatcttctc cgtcatcggc gaagagttcg gcctgctggt
     2401 ctgcggcctc atcgtcgtgc tctatctcgc ccttgtcacc cgcgtcctgc tgcgcctgat
     2461 cgatgaggat gatctgttca cggtccttgc cgccaccggg ctcaccgccc agctcggcgg
     2521 gcaggccttc atcaacatcc tcgtcaatct ccagctgttc ccgtcgaagg ggatgaccct
     2581 gccgctgatc tcctatggcg gctcgtccac cgtggcgctg tgcctgggtg tcgggttcct
     2641 gctggcgatc acccggcgca acccctatct gcagcgccat cgcgtaacct tgcgcgaggt
     2701 gggccgcggc ccgcaagtgc cgagcgccgg cctgaagacc ggcctgccga aggcgggagt
     2761 gctgaaaaaa tgagtggggt ttcccgtcac tatgtcctcg ccgcgggcgg caccggcgga
     2821 cacctgatcc ccgccttcgc gctggcggcg gagcttgacc ggcgcggcca ccacgtcgcc
     2881 ctcgtcacgg atgaacgggg cgccgccatt cccggcaagc ccgatttcct ccccgcccac
     2941 gtcatcccgg cgggccggat cgaaggcatg aacccgctcg gctggctcaa gggcctgcgc
     3001 gccattctcg aaggccgccg catggcgctg cgcctgttcg atagcttcgc gcccagcgcc
     3061 gtcgtcggct tcggcggcta tccggcgctg cccgccgtgc tggcggcaac ctccgccaag
     3121 atccccagca tcgtccacga acagaacgcc gtgctcggcc gggtcaaccg gttcctcgcc
     3181 aagcgggtcg atgccatcgc cacggcctat gccgaggtcg accggctgga ttcgaagctg
     3241 ctcggcaagg tccaccttgt cggcaatcct gtccgtgccg aagtgctcac cctgcgcgat
     3301 cagccctatc ccgagttcac cgaggatggc ctgctgcgcg tgctcgtcac cggtggcagc
     3361 cagggcgccc gcgtcctctc cgaagtcgtg cccgatgggc tgtcgatgct ccagccggcc
     3421 ctgcgcgggc ggctccaggt catccagcag tgccgggcgg aggatgtcga cgcggtgcgc
     3481 cagcgctatg cgggccacgg catccccgcc gaactcgcca cctatttcga ggacatggcc
     3541 gagcggctgg ccgacgccca cctgttcatt ggccgcgctg gtgccagcac cattgccgag
     3601 ctgaccgccg ttggccgccc ggccatcctc gtgcccttgc ccatcgccac cgacgatcac
     3661 caggccgcca acacccgcga gatcgtcgcc gccggcggcg cccgggcgat ccgccaggaa
     3721 cgattcaccg cggtcgaact ggccaagcag atccaggcca tggcccagca tccccacagc
     3781 cttgccaatg ccgcccatgc cgcctggaac tgcggctatc ccaaggccgc ggaggacctc
     3841 gccgatctcg tcgaaagctt tggcgccgcc cccctgatgg acgtgatccg cgtcggcggg
     3901 gacgcgccgg tggcgtcggg gaacgaggcc ttcgcaaggg ataacgcggc atgaaaggcg
     3961 tccccaccga catcggcacg atccacttcg tcggcatcgg cgggatcggc atgtccggca
     4021 tcgccgaagt catgcacaac cttggctaca aggtgcaggg ctccgacatt gccgaaggct
     4081 atgtggtcga aggcctgcgc cagcggggca tcaaggtcat gatcggccac aaggccgaga
     4141 accttggcga agccgccgtg gtcgtgacgt cgaccgccgt caagcgggac aatcccgaag
     4201 tcgtcgccgc gcttgagcac cgcgtccccg tggttcgccg cgcggaaatg ctggccgagc
     4261 tgatgcggct caagaacacc gtcgccgtgg ccggcaccca cggcaagacc accacgacct
     4321 cgatgatcgc cgccctgctc gatgccggcg ggatcgatcc gaccgttatc aacggcggga
     4381 tcatcaactc ctacggctcc aatgcccgcc tcggcgcatc ggactggatg gtggtggaag
     4441 cggacgagag cgacggatcg ttcctccggc tcgacggaac cctcgcagtt gtcaccaaca
     4501 tcgatcccga gcacctcgat cactacggat cgtttgaccg ggtgaaggag tgcttcgtcg
     4561 aattcatcga gaatgtcccg ttctacggcg cggccatgct gtgcatcgat cacccggaag
     4621 tccaggcgat catccccaag gtgcgcgatc gccgcgtcat cacctacggg ttttccgccc
     4681 aggccgacgt gcgcggggag ggagtcactc cggttccggg tggtaaccgc ttcaccgccg
     4741 tgctgcgcgc ccgcgatggc tcgttccgcc ggatcgagga tatcgtcctg cccatgccgg
     4801 gccgccacaa cgtccagaac gcgctcgccg ccgtcgccgt ggccgtcgag atgggctgtt
     4861 cggacgattg catccgcggc ggcttcgcca agttcggcgg cgtcaagcgg cgctttacca
     4921 aggtcggcga aatcgatggc gtcaccatca tcgatgacta tggccaccac ccggtcgaaa
     4981 tccgcgccgt cctcgccgct gcgcgcgaag gggtgaaggg ccgggtcatc gccgtggtcc
     5041 agccgcaccg cttcacccgg ctgcgcgacc acctcgatga tttcgcgggc gccttcaacg
     5101 atgccgacgt ggtctatgcc gcgcccgtct acccggccgg cgagcagccg atcgatggcg
     5161 tcgacagcgc cgcgctggtg gcgggtgcca aggcgcgcgg ccaccgcagc gcccacctga
     5221 tcgccggccc ggcggaattg gcgcgcgatc tggccgcgac cgtgcgcccg ggcgacatgg
     5281 tcgtctgcct cggcgcgggc gatatcacca ggtgggcggc gggcctcgcc ggcgcaatcg
     5341 ccgcggagaa ggcccccgcg tgaccactac gcttgccctc ccgcccgtgc gcggcaagct
     5401 gacggcccag gcgcccctgg ccccgctcgt ctggttcaag gccggtggag ccgcgcagtt
     5461 cttgtttgaa cccaaagata tcaatgactt gcaggaattc ctggctgcgc tggaccccgc
     5521 cgtcccggtc atggccctgg gcctcggctc caacctcatc gtccgcgacg gcggcgtccc
     5581 cggcgtcgtc atccgcctcg gcaagccctt tgcccgcgtg gatgcacttg atcaactaac
     5641 cctctcctgc ggcgccggcg ccagcggcat cctcgtctcc tccaccgccc gcgacgccgg
     5701 ggtggccggc ctcgaattcc tccgctcgat ccccggaacc gtcggcggct tcgtccgcat
     5761 gaacggcggc gcctatggca gcgaggtcaa ggacatcctc gtcgactgcg acgtggtcct
     5821 gcgctcgggg cagctggtga ccctgccggt cgccgatctc cgctacacct accgccacag
     5881 cgaactgccc gaaggcgcca tcgtcgtcgc cgcgcacttc cgcgggcgcc cgggcgagcc
     5941 cgccgcgatc caggccgaga tggaccgcat ctccgccagc cgcgaagcca gccagccgct
     6001 acgctccaag accggcggct ccaccttcaa gaacccgccg ggcgagaagg cctgggaact
     6061 ggttgaccgc gccggctgcc gcggcctcca gctcggcggc gcccaggtca gcgagaagca
     6121 caccaacttc ctcatcaaca ccggcgaggc caccagtgcc gagatcgagg cactgggcga
     6181 ggaagtgcgg cggcgggtaa aggccaattc aggcgtggag ttagagtggg aaatccagag
     6241 ggtgggaaag ccgtgagcct ccccaagttg catgtcgccg tcctgatggg cggctggtcg
     6301 tccgaacgcc cggtcagcct gatgagcggc gagggcgtcg ccaaggcgct ggaagcacgc
     6361 gggcacaagg tcacccgcat cgacatgggc cgcgacgtcg cccagcgcct cgcggaggct
     6421 gcgcccgatg tcgtgttcaa cgccctccac ggtgcgccgg g
//
LOCUS       DATNBV010000443         2495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3453646,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000443 DATNBV010000000
VERSION     DATNBV010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3453646"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VN627_10490"
     CDS             complement(<1..438)
                     /locus_tag="VN627_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03724.1"
                     /translation="MSAMRKAVLAMGLLLLAGRAPAQPAPSASASVPAATPAIAGWDA
                     FVERLRQLGPKMLDRLPPELRSDPQVQQEVGRLMLESLSVGSLAAISGDPDRPVFLPA
                     LNQTINVSQPNADTVYKRATITPGGTYRLRGRTGNIRMARVGQM"
     gene            complement(495..>2495)
                     /locus_tag="VN627_10495"
     CDS             complement(495..>2495)
                     /locus_tag="VN627_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HXD03725.1"
                     /translation="PELVDMTRRLVISAALALPLLVLTMAADLRGAHLLPMRIAPWVQ
                     LGMATPVVLWGGWPFFARGAASLRSGHLNMFTLISLGVGVAYLYSLVATLAPGAFPPQ
                     FRTMGGTVPVYFEAAAMIVALVLLGQVLELRARSATGRAIRALLGLAPKTARLVRGDG
                     SEADVLLETVEPGQVLRVRPGEKVPVDGVVIEGHSALDEAMITGEPLPVEKRVGDRVT
                     GATVNGTGTLLMRAERVGRDTMLAQIVRMVAEAQRSRAPIQALADRVSGWFVPAVVGI
                     AGLTFAAWALFGPEPRLVHALVNAVAVLIIACPCALGLATPMSIMVGTGRGAQAGVLL
                     RNAEAIERLEQIDTVVIDKTGTLTIGKPRLVAVEPLAGFAEADLLALAATLERGSEHP
                     LAAAIIAGAGERGLAPGEVADFRSHTGMGVTGRVNGRQVALGNAALLSRVSAEAAALS
                     AAADARRRRGEGVMLLAVDGVAAGLVAVADPVKPGAGQAVRAMRAEGLRIVMLTGDSR
                     ATAEAVAAEIGGIDEVRAEVLPDQKQAAIAALRARGRIVAMAGDGINDAPALAEADVG
                     IAMGTGTDVAMESAGVTLVGGDLAGIVRARRLGRAVMRNIRQNLFFSFVFNSAGIPLA
                     AGVLYPVFGLLLSPVVAGAAMAFSSVAVIGNALRLRFVHLSG"
BASE COUNT          386 a          909 c          870 g          330 t
ORIGIN      
        1 catctgcccg acccgcgcca tgcggatgtt gccggtgcgg ccgcgcaggc ggtaggtccc
       61 gcccggggtg atcgtcgccc gcttgtagac cgtatcggca ttgggctggg agacgttgat
      121 cgtctggttg agcgcgggca ggaacaccgg gcggtcggga tcgccgctga tggcggcaag
      181 gctgccaacc gaaagcgatt ccagcatcag ccggcctact tcctgctgga cctgcggatc
      241 gctgcgcagc tccggcggca ggcggtccag cattttcgga ccgagctggc gcaggcgttc
      301 gacgaaggca tcccaaccgg cgattgcagg cgtcgcggca ggcacggaag cagaagcgga
      361 cggcgcaggc tgggcgggcg cccttcccgc cagcagcagc agccccatgg ccagcacggc
      421 cttgcgcatc gcggacatcg gattctcccc ggttgtgtct gttgccgata ctgtggtggt
      481 ttttttggcc tgcgtcatcc gcttagatga acgaagcgca gccgcagcgc attgccgatc
      541 acggcgaccg agctgaaggc catggcggca cctgcgacga ccgggctgag gagaaggccg
      601 aacaccgggt agagcacgcc ggccgccagg ggaatgcctg ccgaattgaa gacgaacgag
      661 aagaacaggt tctggcggat gttgcgcatc accgcccggc ccagccgccg cgcccgcacg
      721 attccggcga gatccccgcc gacgagtgtt acgcccgcgc tctccatcgc gacgtcggtg
      781 cccgttccca tggcaatgcc aacatccgcc tcggcgaggg ccggggcatc gttgatgccg
      841 tcaccggcca tggcgacgat ccggcccctg gcccgcaggg cggcgatcgc ggcctgcttc
      901 tggtcgggga ggacttcggc gcgaacctcg tcgattccgc cgatctcggc ggcgaccgct
      961 tcagcggtgg cacggctgtc accggtgagc atgacgatgc gaaggccttc cgcgcgcatc
     1021 gccctgacgg cctgccccgc accgggcttc accgggtcgg caacggccac cagcccggcg
     1081 gccacgccgt ccaccgccag cagcatcacg ccctcacccc gccgccttcg ggcatccgcc
     1141 gccgccgaca gggctgccgc ctcggcgctg acccgggaaa gcagggcggc gttgccaagg
     1201 gcaacctggc ggccattcac gcgcccggtg acccccatgc ccgtatggga gcggaagtcc
     1261 gccacctcgc caggcgccag accgcgctcc ccggccccgg caatgatcgc ggcggccagc
     1321 ggatgctcgc tgccgcgttc gagcgtggcg gccagcgcca gcaggtccgc ctcggcaaag
     1381 ccggccagcg gctcgaccgc gaccagccgc ggcttgccga tggtcagcgt cccggtcttg
     1441 tcgatcacga ccgtatcaat ctgctccagc cgctcgatcg cttcggcgtt gcggaggagg
     1501 accccggcct gtgccccgcg cccggtgccg accatgatcg acatcggcgt cgccagcccg
     1561 agcgcgcagg ggcaggcgat gatcaggacg gccacggcat tgaccagcgc atggaccagc
     1621 cgcggctcgg gcccgaacag cgcccaggcg gcgaaagtca gcccggctat gccgaccacg
     1681 gcggggacga accagccgga gacccgatcg gccagcgcct ggatcggcgc gcgcgagcgc
     1741 tgagcctcgg cgaccatccg cacgatctgc gccagcatgg tatcgcgtcc gacccgctcg
     1801 gcccgcatca gcagcgttcc ggtgccgttg acggtagccc cggtcacccg gtcgccgacg
     1861 cgcttttcga cgggcagcgg ttcgccggtt atcatcgcct cgtcgagcgc gctgtggccc
     1921 tcgatcacca cgccgtcgac cgggaccttc tcgcccgggc gcacccgcag gacctggccg
     1981 ggctcgaccg tttcgagcag gacgtccgcc tcgctgccgt cgccgcgcac cagccgcgcc
     2041 gtcttcggcg cgaggccgag cagggcgcgg atggcccggc ccgtggccga gcgcgcgcgc
     2101 agttcgagca cctgccccag caggaccagc gccacgatca tcgccgccgc ctcgaaatag
     2161 accgggaccg tgccgcccat cgtgcggaac tgcggcggga aggcgccggg cgcgagggtg
     2221 gcgacgaggc tgtagagata ggccacgccg acgccgagcg agatcagcgt gaacatgttg
     2281 agatggccgc tccgcagcga ggccgcgccg cgcgcgaaaa acggccagcc gccccacagg
     2341 acgacgggcg ttgccatgcc cagctgcacc cacggcgcga ttcgcatggg caacaggtgg
     2401 gcgccccgca ggtcggcggc catggtcagc accagcagcg gcagggcgag ggcagcgctg
     2461 atcacgaggc ggcgggtcat gtcgaccagt tcggg
//
LOCUS       DATNBV010000444         4825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4083864,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000444 DATNBV010000000
VERSION     DATNBV010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4083864"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VN627_10500"
     CDS             <1..246
                     /locus_tag="VN627_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXD03726.1"
                     /translation="LWARCGLTRRDRSLVTLGILMALRANSELEYHFRIARANGLTKR
                     EIDEAIYQASGYAGFPAAANASAVYGDVLKADGAAGK"
     gene            complement(444..2390)
                     /locus_tag="VN627_10505"
     CDS             complement(444..2390)
                     /locus_tag="VN627_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HXD03727.1"
                     /translation="MTIGRGAEGAMVDAVPSHTRLAVDDDDGAADPGAHSAVPLPRRP
                     EPQVSAPTNPPEGDPADGDTAPPGRMLALTDFAPPSLSAGERLMRMAYGLGLRGSVSG
                     AFRKPAKKRLLATVESPLAGSKVAGTAIRAGHFLLHGTKLPIDQAELAGVARMSAPME
                     RYVHSFAWAADLTAAASREQGAPLAEKILAAWLDANPKAPARAAKAGAWSVGLTGTRL
                     LCWLVDAPYFLSGSQRTRALGAMADHARWLDRQVARAEDIVAALAGWSAIVAAGLLLP
                     DGKARRLYGEAGLIRTLGELVGDDGGTLTRSPLAQMEAIALIVRVIACYRATRAEPPE
                     ALTGMLGLLVPPLLALTHGDGSLGSWQGGWAVSGDEVARLVAASGVRARPLRDVRQWG
                     YQRIVAGKAVLQFDAAPPPLPRHARTGCASTLAFELSHAGHRMIVNCGGAGAAGGLIP
                     ARLEQGLRATAAHSTLVLDDANSTAVLINGKIGAGVGEVEVDRRMLQPEESEAAARPG
                     HRAPGATRLEASHDGYAARYGLIHRRILILREDGSELRGEDLLVPSRGKGARRGKVAF
                     AIRFHLGPGVEVGRSGDGQGVGLVLPDGTYWQFRAGGEAEVTVEDSLWADAHGRPVGT
                     QQIVVSGLISRGGGNFAWLLKKMG"
     gene            complement(2368..3054)
                     /gene="rpe"
                     /locus_tag="VN627_10510"
     CDS             complement(2368..3054)
                     /gene="rpe"
                     /locus_tag="VN627_10510"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXD03728.1"
                     /translation="MQAKTAPLISPSILSADFARLGEEVRAIDAAGADWIHVDVMDGH
                     FVPNITIGPMVVKALRTHTLKPFDVHLMISPVDTYIEAFAAAGADIITVHPEAGPHVH
                     RTVQLIKSLGKQAGISLNPATPAKTLDYLIDEIDLVLVMSVNPGFGGQSFITSQLRKI
                     EAVRKMIDKSGRDIRLEVDGGVDAVTAPQCVNAGADVLVAGTATFRGGPDAYAANIAR
                     LKGHDDRQGG"
     gene            3257..4135
                     /locus_tag="VN627_10515"
     CDS             3257..4135
                     /locus_tag="VN627_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03729.1"
                     /translation="MNAFATPDLSPIALMRREMSLREGTVPDALGGLPVALGHNRMNA
                     DAFLLREPQVAFLYRRGEGVTVERGEGWSPQLEELYLNGSVCAAVASLNGLLPLHASA
                     VVVGGRVIAFTAPAGGGKSTLAAGLTSLGLPLFCDDTLVVNLADESAVLCLPGHKRMK
                     LWPDAVALTGSTALEQVSADYAKFYVTPAGSEVAEVLPLGALVFLEESAPDLREPALV
                     AVEGGEKIARLSDDHYTREMFREANGLSGPALFALQARIARGLPMARFVRPLARERFG
                     ETTRFLADALCAMMGR"
     gene            4132..4395
                     /locus_tag="VN627_10520"
     CDS             4132..4395
                     /locus_tag="VN627_10520"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HXD03730.1"
                     /translation="MSAIVKIAANYVETDIDDETVLMRLSDGDFFALEGTGQAIWQAI
                     DGRRSRDAIVALLADQFEADPALLGADVDRFLEDLAGAGLVGA"
     gene            4382..>4825
                     /locus_tag="VN627_10525"
     CDS             4382..>4825
                     /locus_tag="VN627_10525"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HXD03731.1"
                     /translation="MSAPETALRWRHRARVAEAMVLLVAARALVAGVSFRHWRGWFGE
                     VAATPGQARLDEAAALRARRVGRAVNQAARHLPLHCKCLPQAMAASWMLRRRAIPGEI
                     VFGVLDSGRRGTPGDLHAWARCGGYTIAGEGEPAHNPVLVLTRMVK"
BASE COUNT          717 a         1594 c         1771 g          743 t
ORIGIN      
        1 ctgtgggcgc gctgcggcct gacccggcgc gaccgcagcc tggtgacgct gggcatcctc
       61 atggccttgc gcgccaacag cgagctggaa taccacttcc gcatcgcccg cgcgaatggc
      121 ctgaccaagc gcgagatcga cgaggcgatc taccaggcca gcggctatgc cgggttcccg
      181 gcggcggcca atgcgagcgc ggtgtatggg gacgtgctga aggcggatgg ggctgcaggg
      241 aagtaggtct gctggcgaag gtcgacggca gacacgggcg acaacctggt gtcgccgcgc
      301 atgattcctc cccacttgtg gggaggtggc agccgaaggc tgacggaggg ggtattcagg
      361 cgggcgctgc gctgcgcggg agagccccct ccaccgccgt tccgggggtt cccctcccca
      421 cttgtgggga ggattggcgc cctctagccc attttcttga gcagccaggc aaagttgccg
      481 ccgccgcggc tgatcaggcc cgagacaacg atctgctggg tcccgacggg acggccatgg
      541 gcatcggccc agagcgaatc ctcgaccgtc acctccgcct cgccgccggc gcggaattgc
      601 cagtaggtgc cgtcgggcag gacgaggccg acgccctggc catcgcccga gcggccgacc
      661 tcgacgccgg ggccgaggtg gaaacggatg gcaaaggcga ccttgccgcg ccgggcgccc
      721 ttgccgcgcg aggggacgag caggtcttcc ccgcgcagtt cgctgccgtc ttcccgcagg
      781 atcaggatgc gccggtggat caggccgtag cgggcggcat agccatcatg gctggcttcc
      841 agccgggtgg cgccgggcgc gcggtggccg ggcctggcgg cggcttcgct ctcttccggc
      901 tggagcatgc ggcgatcgac ctcgacctcg cccacgccgg cgccgatctt gccgttgatg
      961 aggacggcgg tggaattggc atcgtccagc acgagggttg aatgggcggc cgtggcgcgc
     1021 aggccctgtt cgagccgggc cgggatcagc ccgcccgccg cgcccgcccc gccgcaattg
     1081 acgatcatcc ggtgtccggc gtggctcagt tcaaaggcga gggtggaagc gcagccggtg
     1141 cgcgcgtggc gcggcagggg cggcggggcg gcatcgaact ggaggacggc cttgccggcg
     1201 acgatccgct ggtagcccca ttggcgcacg tcgcgcagcg gccgggcgcg cacgcccgaa
     1261 gcggcgacaa ggcgggcgac ctcatccccc gagacggccc agccgccctg ccacgagccc
     1321 agcgagccat cgccatgcgt cagggcaagc agcggcggaa ccagcaggcc gagcatgccg
     1381 gtgagcgcct cgggcggttc ggcgcgggtc gcccggtagc aggcgatcac ccggacaatg
     1441 agggcgatgg cctccatctg ggcgagcggg ctgcgggtga gcgtgccgcc atcgtcgccc
     1501 accagttcgc ccagcgtacg gatcagtccg gcctcgccat agagccggcg ggccttgcca
     1561 tccggcagga gcagcccggc ggcgactatg gccgaccatc cggccagcgc cgcgacaatg
     1621 tcctccgcgc gggcgacctg gcggtcgagc cagcgggcat gatcggccat ggcgcccagc
     1681 gcgcgcgtgc gctggctgcc ggagaggaaa tagggagcat cgaccagcca gcacagcagg
     1741 cgggtcccgg taagccccac ggaccacgcc ccggccttgg cggcgcgggc gggggccttg
     1801 gggttggcat cgagccaggc ggcgaggatc ttctcggcga gcggcgcgcc ctgttcgcgg
     1861 ctggcggcgg cggtaagatc ggcggcccag gcgaaggaat ggacatagcg ctccatcggc
     1921 gcgctcatcc gggcaacgcc ggcgagttcg gcctggtcga tcggaagctt ggtgccgtgg
     1981 agcaggaagt ggccggcgcg gatcgccgtg ccggcgacct tgctgcctgc gagcgggctc
     2041 tcgaccgtgg ccagcaggcg cttcttcgcg ggcttgcgga aggcgcccga gacactgccg
     2101 cgcagcccga ggccataggc catgcgcatc agccgctcac ccgccgagag gctgggcggg
     2161 gcgaaatcgg tgagggcgag catgcggccc ggaggggccg tatcaccatc ggcgggatcg
     2221 ccttcgggag gattcgtcgg cgcgctcact tgcggctccg ggcggcgggg caggggcacg
     2281 gcggaatggg cgcccgggtc agccgcgccg tcatcgtcat caaccgcgag gcgggtgtgg
     2341 gacgggacgg catcgaccat ggcgccctca gcccccctgc cgatcgtcat ggcccttgag
     2401 ccgagcgatg ttggcggcgt aggcgtcagg cccgccgcgg aaggtggccg tgccggcgac
     2461 cagcacgtcg gcccccgcgt tcacgcattg cggggcggtg accgcatcga cgccgccatc
     2521 gacttccagc cggatatctc gccccgactt gtcgatcatc ttgcggacag cctcgatctt
     2581 gcgcagctgg ctggtgatga agctctgccc gccaaagccg gggttgacgc tcatcaccag
     2641 gaccaggtcg atctcgtcga tcaggtagtc cagcgtcttg gccggggttg cggggttgag
     2701 cgaaatgccg gcctgcttgc ccagcgactt gatcagctgg accgtgcggt ggacgtgggg
     2761 gcccgcttcg ggatgaacgg taatgatgtc tgcgcccgcg gcggcgaagg cctcgatata
     2821 ggtgtccacc gggctgatca tcaggtggac gtcgaacggc ttgagcgtgt gggtgcgcag
     2881 ggccttcacc accatcgggc cgatggtgat gttgggcacg aagtggccgt ccatcacgtc
     2941 gacatggatc cagtctgccc cggcggcatc gatggcgcgc acttcctcgc caaggcgggc
     3001 aaagtcagcc gagaggatcg agggcgagat caggggcgcg gtcttggcct gcacggttat
     3061 tgggcctgca caaaaattgg ggacgttccc ccggcgttag acaccacatc ttgtggccac
     3121 aaccgccgaa tgcgccgttt tccacacagt ccctccgaat cgaatcaata tccgggcgaa
     3181 ccgaagcgat gttcgcgcca attgcgccag accggcgatt gaccggcgcg gggcggcggc
     3241 tttacccacg cccgacatga atgccttcgc tacgcccgac ctgtcgccca ttgccctgat
     3301 gcgccgggag atgtcccttc gcgaagggac ggtgcccgat gcgctcggcg gcctgccggt
     3361 cgcgctcggg cacaaccgga tgaatgccga cgcgtttctg ctgcgcgaac cgcaggtggc
     3421 gtttctttat cgccggggcg agggcgtgac ggtcgagcgg ggcgagggat ggagcccgca
     3481 gcttgaggag ctctatctca acggcagcgt ctgcgccgcc gtggcgagcc tcaacggcct
     3541 tttgccgctc cacgcctcgg ccgtggtcgt gggcggccgg gtgatcgcct ttaccgcgcc
     3601 cgccggcggc ggcaagtcga ccctcgccgc cgggctcacc agccttggcc tgccgctgtt
     3661 ctgcgacgat acgctggtgg tgaacctggc ggacgagagc gcggtactgt gcttgccggg
     3721 gcacaagcgg atgaagcttt ggcccgacgc cgtggcgctg accggaagca ctgcgctgga
     3781 acaggtcagc gcggattacg caaagttcta cgtcacgcct gccggcagcg aggtggccga
     3841 agtgctgcca ctgggcgctc tggtgttcct tgaggaaagc gcgcccgatc tgcgtgagcc
     3901 ggccctggtc gcggtcgaag gcggcgagaa gatcgcccgg ttgagcgacg atcactatac
     3961 ccgcgagatg ttccgcgagg cgaacggcct gagcggcccg gcgctgttcg ccctccaggc
     4021 gcgaattgca cggggcctgc cgatggcgcg gttcgtccgt ccgctggcgc gcgaacggtt
     4081 tggcgaaacg acccgcttcc ttgccgatgc gctttgcgcc atgatgggcc gatgagcgcc
     4141 atcgttaaaa ttgcagccaa ttacgtcgag accgacatcg atgacgagac ggtgctgatg
     4201 cgcctctccg acggagattt ctttgcactc gagggcaccg gacaggccat ctggcaggcg
     4261 atcgacggta ggcgcagccg cgatgcgatc gttgccttgc tggctgacca gttcgaagcc
     4321 gacccggccc tgctcggggc cgatgtcgac cgctttctcg aggacctcgc cggagcgggg
     4381 cttgtcggcg cctgagacag cactgcgctg gcggcaccgg gcccgtgtcg ccgaagcgat
     4441 ggtcctgctg gtggcggcgc gggcgctggt ggccggggtg tcgttccggc actggcgcgg
     4501 ctggttcggc gaagttgcgg ctacccccgg ccaggcccgg ttggacgagg cggccgcctt
     4561 gcgcgcccgc cgggtcggac gggcggtgaa ccaggcggca aggcacctgc cgctccattg
     4621 caaatgcctg ccccaggcta tggcggcatc gtggatgctc cgccgccgtg caatccccgg
     4681 cgagatcgtc tttggggtgc tcgattcggg acggcgcggg acgccgggcg atctccacgc
     4741 ctgggcccgc tgcggcggat ataccattgc cggagaaggg gaacccgccc acaatccggt
     4801 tcttgtcctc acgcgcatgg ttaag
//
LOCUS       DATNBV010000445         3578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1232685,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000445 DATNBV010000000
VERSION     DATNBV010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232685"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..791)
                     /locus_tag="VN627_10530"
     CDS             complement(27..791)
                     /locus_tag="VN627_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03732.1"
                     /translation="MSGKVIAITGANGGLGRATSRRFAADGDTVIMLGRNLAKVTEAA
                     SQIAGDVWPLQCDIGNPASVREAFAQIAERHAVLDVLINNAGNFQAVAFAEASEEQII
                     DSMNGNFLGPVLCSRAAIPLMKAGSKLIHVTSEAVDIVFAMLSMYGAGKAGLERFSRS
                     LHQELIDSGTGIRSMVYRAGQMCGEGTSTTIDEENVGRFADWNAKLGLRPMERGFTSY
                     ATAADLLHSMANLPDDAGVEIVKCFSVPGIVGNSAG"
     gene            969..2021
                     /gene="pyrC"
                     /locus_tag="VN627_10535"
     CDS             969..2021
                     /gene="pyrC"
                     /locus_tag="VN627_10535"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXD03733.1"
                     /translation="MTITASSDLVIRRPDDWHVHLRDGAMLEAVAGYTARQFGRAIVM
                     PNLTPPAATTAEVAAYRDRIMAALPGSDFRPLMALYLTDGTDPDDVEQGFALGIVTAA
                     KLYPAHATTNSAHGVTNVRNIYPVLDRMQEIGMPFLVHGEVTDGDIDIFDREAVFIER
                     VMQPVLRDFPALKVVFEHITTRDAADFVENAGPNVGATITPHHLMINRNALFAGGLRP
                     HAYCLPVAKREVHRLALRKAATSGSAKFFLGTDTAPHQIAAKESACGCAGIFCAPHAL
                     ESYATVFEEEGALDRLEAFASENGPRFYGLPLNEGRVRLERAECLVPEQIPAAGGAVV
                     PWLAGETLRWRFAGAA"
     gene            2044..2781
                     /locus_tag="VN627_10540"
     CDS             2044..2781
                     /locus_tag="VN627_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXD03734.1"
                     /translation="MGKLLLASIHDVGPRFESEVDRLVDLLGSVVGEARFAMLVVPDH
                     WGLAPLGEAAAFRARLRGWASLGVEMFVHGWYHKDMAEHSGLTSFKARHMTAGEGEFL
                     GLDEAEAARRMADGRALIEDAIGGETAGFIAPAWLYGPGALAALAASGFPLAEDHLKV
                     WDPRSGDVLARGPVVTWASRSRGRQLSSLGVAALARVAFHGLSVARVAVHPGDTTVPS
                     LLDSIRRTYASFARRRPAGRYADLRTL"
     gene            3021..>3578
                     /locus_tag="VN627_10545"
     CDS             3021..>3578
                     /locus_tag="VN627_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03735.1"
                     /translation="MQAIEPVYQDTPGYPEGFAPGELAASAAAAAEKGGGLPGKALFG
                     LAGVVLSVIVLFAAVFAYRDLQIGTIGALLPRSPLFWALFVLYYLHGPLMEWIIFRRL
                     WNVPFLSGLAALVRKLVSNELVLGYLGEAQFYAWARARGTMAAAPFGAVKDVAILSAL
                     TGNAVTLIMLVFAWPLISSGQLGLPT"
BASE COUNT          540 a         1150 c         1219 g          669 t
ORIGIN      
        1 cgtgctggac ggcggcccta cgtctgtcag ccggcggagt tcccgacgat gcccggcacc
       61 gagaagcact tcacgatttc cacgccggca tcgtcgggaa ggttggccat ggaatggagc
      121 agatcggcgg cggttgcgta actcgtaaag ccgcgttcca ttggtctcag cccgagcttc
      181 gcgttccagt cagcgaaacg cccgacgttt tcttcgtcga tggtcgtgga cgtgccttcg
      241 ccgcacatct gccctgcccg gtaaaccatg ctgcgaatgc cggttccgga atcgatgagt
      301 tcctgatgca gcgagcgcga gaagcgctcg agcccggcct ttccggcacc gtacatcgac
      361 agcatggcga agacgatatc caccgcttcc gacgtgacgt gaatcagctt gctgccggcc
      421 ttcatcaagg ggatcgcggc acgcgagcag aggaccggac caaggaagtt gccgttcatg
      481 gaatcgatga tctgctcttc actggcttcg gcgaaggcca cggcctggaa gttgccggcg
      541 ttgttgatca ggacgtcaag cacagcgtgg cgctcggcga tttgcgcgaa ggcctccctg
      601 acagatgccg ggttcccgat gtcgcactgc agcggccata catccccggc gatttgcgac
      661 gcggcctcgg taaccttggc gagattgcgg ccgagcatga ttaccgtatc cccgtcggcg
      721 gcgaaacgcc gcgacgtggc ccggcccagg ccaccattgg cgccggttat ggcgatcacc
      781 ttgcctgaca ttgcccactc caaaattatc tgaactgatc ggtttgctga tgcctgtttc
      841 cacgcggcgg gcgacttggc aagtccccgc gccgccaccc ggcctttccc gactggaggg
      901 cgatggcacg gacctgcggg atgcaaacgc ggtttggcgg attgccgcgg ctcggctatg
      961 ggcgcgccat gaccatcact gcttcgagcg acctcgtcat ccgccgaccc gatgactggc
     1021 acgtccacct gcgcgacggc gccatgctgg aagccgtggc gggctatacc gcgcgccagt
     1081 tcggccgggc gatagtgatg cccaacctta cgccgcccgc cgccaccacg gccgaggtcg
     1141 ccgcctatcg cgaccggatc atggcggcgc ttcctggcag cgacttccgc ccgctgatgg
     1201 cgctctacct taccgatggc accgatcctg acgatgtcga gcaaggcttc gcgctgggca
     1261 tcgtcaccgc ggccaagctc tatcccgctc atgccacgac gaattcggct cacggcgtga
     1321 cgaacgtgcg caacatctat ccggtcctcg accggatgca ggagatcggc atgccgttcc
     1381 tggtccatgg cgaggtcact gacggcgaca tcgacatctt cgaccgcgag gccgtgttca
     1441 tcgagcgggt gatgcaaccg gtgctgcggg atttcccggc actgaaagtg gtgttcgagc
     1501 atatcacgac cagggacgcg gccgatttcg tggagaacgc tggcccgaac gtgggcgcca
     1561 cgatcacccc gcatcacctg atgatcaatc gcaacgcgct gtttgccggc gggcttcggc
     1621 cgcatgccta ttgcttgccg gtagccaagc gcgaggtgca ccggctggcc ctgcgcaagg
     1681 cggcgacgtc tggcagcgcc aagttcttcc tcgggaccga taccgcgccg caccagatcg
     1741 cggccaagga atcggcttgc ggctgcgccg gcatcttctg cgcgccacat gccctggaga
     1801 gctatgcgac cgtgttcgag gaagagggcg cgcttgaccg gctggaagcc ttcgcctccg
     1861 aaaacggacc gcgcttctat gggctgccgc tgaatgaggg ccgggtacgg ctggagcggg
     1921 cggagtgcct cgtgcccgag cagattcccg ctgccggcgg cgcggttgtc ccgtggctgg
     1981 cgggcgagac gctgcgctgg cgctttgccg gagcggcctg agccgggcct gagggaattg
     2041 cggatgggca agctcctcct cgcctcgatc cacgatgtgg ggccgcgctt cgaaagcgag
     2101 gtggaccggc tcgtcgacct gctcggcagc gtcgtgggcg aggcgcgctt tgccatgctg
     2161 gtggtgcccg atcactgggg gctggccccg ctgggcgagg ccgcggcgtt ccgtgcccgc
     2221 ctgaggggat gggcgagcct gggcgtcgag atgttcgttc acggctggta tcacaaggac
     2281 atggccgagc attcgggcct gaccagtttc aaggcacgcc acatgaccgc gggggaaggg
     2341 gagttccttg gcctggacga agccgaggcg gcgcggcgca tggccgatgg ccgggccctg
     2401 atcgaggatg ccatcggcgg cgagacggca gggttcatcg cgccggcctg gctttatggc
     2461 cccggcgccc tggcggcgtt ggcggcaagt ggttttccct tggccgagga tcacctgaag
     2521 gtgtgggacc cgcgcagcgg cgacgtcctg gcgcgcggtc cggttgtcac ctgggcgagc
     2581 cgcagccggg gccggcaact gagttcgctc ggcgtagccg cgctggcgcg ggtggcgttt
     2641 catgggctga gcgtggcgcg cgttgcggtg catcccgggg atacgaccgt accgtccctg
     2701 ctcgattcca tccgccgcac ctatgccagc ttcgcccggc gccgtccggc ggggcgctac
     2761 gcggatctgc gaacgcttta aaaatccttc tatttcaatt ttttgttcct ggtcatgggg
     2821 tgaactcgcg ttaacctatc cgattgcagc ttgcaccggg ccggggggcg actaaacacc
     2881 ggcggcgtaa ttcgcgctac gcgagccagt aatgcgccgg agccgggagg ggatcgtccg
     2941 gctcggcggg gtcggagggg tacggccgcg ggtctcgtct gcaggattat gtggcgcagc
     3001 aacttgtgac acatcgggac atgcaggcta tcgaacccgt ctatcaggac actccgggct
     3061 atcccgaggg ctttgccccg ggcgagctgg cggcttcggc ggcggcggcg gccgagaagg
     3121 gcgggggctt gcccggcaag gctctgttcg ggctggccgg cgtggtcctt tcggtgatcg
     3181 tcctgttcgc cgccgtcttc gcctaccgcg atctccagat cggcacgatc ggggccctct
     3241 tgccgcgcag cccgctgttc tgggctctct tcgtcttgta ctatctgcac ggcccgctga
     3301 tggaatggat cattttccgc cggttgtgga acgttccgtt cctgtcgggc ctggcggcgc
     3361 tggtgcgcaa gctggtcagc aacgaactgg tcctgggcta tctgggcgag gcgcagtttt
     3421 atgcctgggc gcgggcccga gggaccatgg cggcagcacc gttcggcgcg gtcaaggacg
     3481 tcgccatcct ctcggccctg acgggcaatg ccgtcaccct gatcatgctg gtcttcgcct
     3541 ggccgctgat ttcctcgggc cagctcggct tgccaacg
//
LOCUS       DATNBV010000446         4257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_559496,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000446 DATNBV010000000
VERSION     DATNBV010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559496"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VN627_10550"
     CDS             complement(<1..786)
                     /locus_tag="VN627_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HXD03736.1"
                     /translation="MITRSVSATGFGQSLGGRQWLWRGGNMALEDEAAIALGDDLLDQ
                     LLLARGVARADLARHRRPTLREFLPDPAEFRDMIAAAERLAQAVVGGEKVTVYGDYDV
                     DGATSAALLLRLLEQLGLPSRYYIPDRLLEGYGPSAEALVRLAEEGSSLIVTVDCGAM
                     AHEALARAHEAGVDVIVVDHHKCPPDLPRAAALVNPNRLDESDVGLAHGHLAAVGVAF
                     LLAVETVRRLRAAGYFAARAEPDLFGLLDLVALGTVADVAALHG"
     gene            878..1384
                     /locus_tag="VN627_10555"
     CDS             878..1384
                     /locus_tag="VN627_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXD03737.1"
                     /translation="MNAGPEPQLAIVWHSRTGASEQLAAAAALGAAESGEGACRLLRA
                     EDCTPADLLAAAGYLFVCPENLGTMSGAMKELFDRAYYPLLGQVEGRPYATIIAAGSD
                     GRGAQAQIDRIVTGWRLRRVAEPMIVNLDAQTPEKILTLKQVGKAERDAAKHLGQALS
                     VGIALGVF"
     gene            1510..2268
                     /locus_tag="VN627_10560"
     CDS             1510..2268
                     /locus_tag="VN627_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03738.1"
                     /translation="MRREAGLTLYFPPSGASAGEAAPGRPDARAIQELAGRSGASVAF
                     SVSHLPKDHPYWVELLALGLTFDLRGLAPGIDARVAAPVHAFGLPKPEPRALEGIVLR
                     PGPHLSSAAALLPVVRTMAALGCELAALPGLVGIGWEPAGSIMAPDYFRSTVGSWLND
                     GPFPALGFTALARSPQGAMRSVGLGLFLGHELELAPLPGESPADAARLAVRLIHDLVQ
                     NGSYTPGDQYGPGGEVLHCSFNADLSLMLIGRTG"
     gene            2373..2882
                     /locus_tag="VN627_10565"
     CDS             2373..2882
                     /locus_tag="VN627_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AHH domain-containing protein"
                     /protein_id="HXD03739.1"
                     /translation="MPFRAVNRAGSSGYDAGLQRHHLLPHQLTGVSTLGRLFDRLGRQ
                     AIGFDDFRRNGLLLPASEAAARILGLPLHRGPHRDYNRMVIDRVGLIEQSWSRERWRR
                     GDSALEAASFRLRLLQQALRRRLLEGGRRRVVLNGRDPALADAAFAELDAMADLLWSE
                     AEAADPAGW"
     gene            complement(2908..>4257)
                     /locus_tag="VN627_10570"
     CDS             complement(2908..>4257)
                     /locus_tag="VN627_10570"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HXD03740.1"
                     /translation="LIATSFESLAGVHSAIVHVYNAVSPLWRQVVFGLEQPQIREIAS
                     NAAKMLRDNAAKHPQTDWHFEYSPETFSTAELDFSIECCEAVMQILQPTPEKPIILNL
                     PATVEAATPNIYADQIEYFCRNLPNRESAVISLHTHNDRGTGVAAAELGLLAGADRVE
                     GCLFGNGERTGNCCLVTVGLNMYTQGVNPQLDFSDIDEVIQTVEYCNQLPVHERHPYG
                     GELVYTAFSGSHQDAIKKGFAAQETRNDQLWAVPYLPIDPADLGRSYEAVIRVNSQSG
                     KGGFAWVIEQDQGLKLPKRMQAHFSRYVQDLADELGRELQASDIWDVFRKTYRLDEPQ
                     HFQLLDYNEMRSPDGSRVFAGKIGVNGKAQTVSGRGNGLISSVVATLAETFGVALEIR
                     DYSEHALGKGADSRAAAYVECTAADGRTVWGVGIDEDVATAGVRAVLSAANAVAGAK"
BASE COUNT          640 a         1423 c         1459 g          735 t
ORIGIN      
        1 cccatggagc gcagccacat cggccacggt ccccagcgcg accagatcga gcaggccgaa
       61 caggtctggc tcggccctcg ccgcaaaata gcccgccgcg cgcaggcgtc gcacggtttc
      121 caccgccagc aggaaggcca cgccgacggc agcgaggtgg ccgtgggcga ggcccacgtc
      181 gctctcgtca agccgattcg ggttcaccag cgccgcggcc cggggcaggt cgggcgggca
      241 cttgtggtga tcgaccacga tcacgtcgac cccggcctcg tgcgcccggg ccagtgcttc
      301 gtgggccatg gctccgcagt ctaccgtaac gatcaggctg gaaccttcct ctgccagccg
      361 caccagcgcc tcggccgagg ggccgtagcc ttccagcagg cgatcgggga tatagtagcg
      421 cgagggcagg ccgagctgtt cgagcaggcg cagcaggagc gcggcgctgg tcgcgccatc
      481 gacgtcgtag tcgccgtaga ccgtgacctt ctcgccgccg accaccgctt gggccagccg
      541 ctcggcagcg gcgatcatgt cgcgaaactc ggccgggtcg ggcaggaatt cgcgcaaggt
      601 cgggcggcgg tggcgagcca ggtccgcgcg cgccacgccg cgggcgagca gcaactggtc
      661 gagcagatca tcgcccaggg cgattgcagc ctcgtcctcc agcgccatgt tgccgccgcg
      721 ccagagccac tggcggccgc ccagcgattg cccgaaaccc gtggcggaaa ccgaacgcgt
      781 gatcattcgt cggggctaac ccattgcacg gctaacggga agggttcccc cggcgcgcgg
      841 gtgttgatag cccgggggaa tggcgcaggc caaacacatg aacgccggac cggagccgca
      901 gctggcgatc gtctggcaca gccgcaccgg tgccagcgag cagctggccg ccgccgccgc
      961 gctaggggcg gcggaatctg gtgaaggtgc ctgccgcctg ctccgcgcgg aggactgtac
     1021 ccccgccgat ctgcttgccg ccgccggcta cctgttcgtg tgccctgaaa acctggggac
     1081 aatgtcgggg gcgatgaaag agctgttcga ccgggcctac tatcccctgc tcggccaggt
     1141 ggaaggccgg ccctatgcca cgatcattgc cgccggttcg gatggccgcg gcgcccaggc
     1201 ccagattgac cgaatcgtga ccgggtggcg ccttcggcga gtcgccgagc cgatgatcgt
     1261 caacctcgac gcgcagaccc cagaaaagat actgacactc aagcaggtag gaaaagctga
     1321 gcgcgatgcc gccaagcatt tggggcaggc gctttctgtg gggattgcac tcggtgtctt
     1381 ctgagtacga gaccggtcca aaacggtacg gccgggtcac cgggactcga cgggcgatta
     1441 ttttgtgcca ttggtctgtc gatggacagg gcaggtggaa ggttcgcctc gtggcaggag
     1501 ggcattgcag tgcgccgtga ggcgggtctg acgctgtatt tcccgccctc cggagcaagc
     1561 gctggtgagg cggcgcccgg ccggcccgat gcccgcgcga tccaggaact ggctggccga
     1621 tcaggcgcgt cggttgcctt ctccgtcagc cacctgccca aggatcatcc ctactgggtc
     1681 gagttgctgg ccctcgggct gactttcgac ctgcggggcc tggcccccgg catcgatgcc
     1741 cgtgttgccg cgccggtcca cgccttcggc ctgccgaagc cggagccgcg cgctctcgaa
     1801 gggatcgtgc tgcggcccgg cccgcatctg tcttccgccg ccgcgctgct gccagtggtt
     1861 cgcaccatgg ccgcgttggg ctgtgaactt gccgctctgc ccggcctcgt cgggatcggc
     1921 tgggaaccgg ccggctcgat catggcgccc gactattttc gcagcaccgt cggctcctgg
     1981 ctgaatgacg gaccattccc ggcgctgggg ttcaccgcgc tcgcccgttc gccccaaggc
     2041 gcgatgcgct ctgtcggtct cgggctcttt ctcggtcacg agctggaact ggcgcccctg
     2101 cccggagaat cgccagcgga tgcggcgcgg ctggccgtgc ggctgattca cgatttggtg
     2161 cagaatggtt cctatacccc gggtgaccaa tatggtcccg ggggagaagt cctgcattgc
     2221 agcttcaacg cagacctgag cctgatgctg atcggcagga ctggctgacc gctcgctatc
     2281 gcgaggcttc gggttctcct tgccgaccgg cccagccgtg cctgcccctg ccgcccattg
     2341 ctgcgcagga agcattccgc gtccgcgcca atttgccgtt tcgcgcggtc aaccgggcgg
     2401 ggagcagcgg ctacgatgcc ggcttgcagc gccatcatct cctgccccac cagctgaccg
     2461 gcgtttccac actcgggcgg ctgttcgacc ggttgggccg ccaggcgatc gggtttgacg
     2521 atttccggcg caatggcctg ctcctgccgg caagcgaagc ggcggcgcgg atcctgggcc
     2581 tcccgctgca ccgcgggccg caccgcgatt ataaccgcat ggtgatcgac cgggtgggcc
     2641 tgatcgagca atcctggtcg cgcgagcgct ggcggcgcgg cgacagtgcg ctcgaggcag
     2701 cctcgttccg cttgcgcctc ctgcagcagg cgctgcggcg ccgattgctg gaaggcggtc
     2761 ggcggcgagt ggtgctgaac ggacgggacc cggcgctcgc cgatgccgcc tttgccgaac
     2821 ttgatgccat ggcagacctg ctgtggagcg aggccgaggc ggccgatccg gccgggtggt
     2881 gagacccgtg ccggccgccc tgcttgatta cttcgcgccc gcgacggcgt tcgccgcgct
     2941 aaggacggcg cgcacaccag ccgtggccac gtcctcgtcg atgcccacgc cccagacggt
     3001 gcggccgtct gccgcggtgc attcgacata ggccgccgca cgggaatcgg ctcccttgcc
     3061 gagcgcgtgc tcggaatagt cacggatttc cagcgccacg ccgaaggttt ccgccagcgt
     3121 tgcgacgacc gacgagatca ggccgttgcc ccgcccggac acggtctgtg ccttgccgtt
     3181 gacgccgatc ttgccggcaa agacccggct gccgtcggga ctgcgcattt cgttgtagtc
     3241 gagcagctgg aaatgctgcg gctcgtcgag gcggtaggtc ttgcggaaca cgtcccagat
     3301 gtcgctcgcc tgcagttcgc ggccaagctc gtcggccagg tcctgcacat agcggctgaa
     3361 gtgggcctgc atccgcttgg gcagcttgag cccctgatcc tgctcgatca cccaggcgaa
     3421 gccgcccttg ccgctctgcg agttgacgcg gatcactgcc tcgtagctgc ggccaaggtc
     3481 tgccgggtcg atcggcaggt agggcacggc ccagagctgg tcgttgcggg tttcctgcgc
     3541 ggcgaagccc ttcttgatcg catcctggtg gctgccggaa aatgcggtgt agaccaattc
     3601 cccgccatag ggatggcgtt cgtggactgg cagctggttg cagtattcaa ccgtctggat
     3661 cacctcgtcg atgtccgaga agtccagctg cgggttgacg ccctgcgtgt acatgttgag
     3721 gccgaccgtc acgaggcagc agttgcccgt gcgctcgccg ttgccgaaca ggcagccctc
     3781 cacccggtcc gctcccgcca acaggcccag ttcggccgcg gcaacgccgg tgccgcggtc
     3841 gttgtgggtg tggagcgata tcaccgcgct ttcgcggttc ggcagattgc ggcagaaata
     3901 ctcgatctgg tcggcataga tattgggcgt cgccgcctcg accgtggccg gcaggttgag
     3961 gatgatcggc ttctcgggcg tcggctgcag gatctgcatc acggcctcgc agcattcgat
     4021 cgagaaatcg agctcggcgg tggagaaggt ttccggcgaa tattcgaagt gccagtcggt
     4081 ctgcggatgc ttcgcggcat tgtcgcgcag catcttggca gcattgctgg cgatttcgcg
     4141 gatctgcggc tgctccagcc cgaacacgac ctgccgccac agcggggaga ccgcgttgta
     4201 gacatggacg atggccgagt ggacgccggc aaggctctcg aacgaggtgg cgatcag
//
LOCUS       DATNBV010000447         2405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_265969,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000447 DATNBV010000000
VERSION     DATNBV010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265969"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VN627_10575"
     CDS             complement(<1..185)
                     /locus_tag="VN627_10575"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HXD03741.1"
                     /translation="MQRFVAWHERGEYACTGSCFDIGIATRAALQRFKQTGDPVAGST
                     DPMSAGNGSLMRLAPAA"
     gene            complement(527..616)
                     /locus_tag="VN627_10580"
     tRNA            complement(527..616)
                     /locus_tag="VN627_10580"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(580..582),aa:Ser,seq:cga)
     gene            1170..1715
                     /locus_tag="VN627_10585"
     CDS             1170..1715
                     /locus_tag="VN627_10585"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXD03742.1"
                     /translation="MAALAAPAVRADVLEIGPSGASWIAGGPAPDTGPAVEGSTAAPA
                     YTQVAATAGPDAWRAHVAVLAERYDLSPALLDAVVWQESRWNPRAVSPAGARGLAQLM
                     PATGRQLGVDWRDPLANLEGGARYLRQQLDRFGGDVEKALAAYNAGPARVARAGSVPP
                     IRETQAYVASILARLSQPVRR"
     gene            1719..2024
                     /locus_tag="VN627_10590"
     CDS             1719..2024
                     /locus_tag="VN627_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HXD03743.1"
                     /translation="MRAARLLALIPALVPGTAMAQAVAANPQGSGPIVAALGWLQGTL
                     LGNVATAVAVMAVAAVGFMMLTGRMNWRFGATVIIGTFILFGAGAIVSGIQSVAVGG"
     gene            2030..2329
                     /locus_tag="VN627_10595"
     CDS             2030..2329
                     /locus_tag="VN627_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HXD03744.1"
                     /translation="MSPAPTPALARFPVHRALTWPQMFAGVTYNFFIINAALTTEAFL
                     ITRSFLALPLALLIHTIGYFACLREPRVFDLWLTKVSRCPRVPNWRRWGCNSYAP"
BASE COUNT          351 a          883 c          763 g          408 t
ORIGIN      
        1 atcgcggcgg gtgcaaggcg catcaggctg ccattgcccg cgctcatcgg gtcggtcgaa
       61 cccgccaccg gatcgccagt ctgcttgaac cgctggagcg cggcccgggt ggcgatgccg
      121 atatcgaagc aggagcccgt gcacgcgtac tcgccgcgct catgccaggc gacgaaccgc
      181 tgcatcaggt cggtctcgtc gagcagcgga tgggcggcca ggctggcgca gtcctcggcg
      241 agctgcgcgt gccggccaag gtccgtaatg cgccagctgt ttgcataccg cccagcatcg
      301 tccgaaccga attcccgaac ttcgagttcg aatgtccgag tgcacccgga tgggattcga
      361 acttcgctct gagcggtttt cgctctctcc tccgaaacgc aaccgggcta ttacaccgta
      421 acggcaggcg ggcgccctcc ggaggtgtaa acgacgccgc gaaaccccgg attcccgcgg
      481 gattaggggc gccgggtcgg cgaagtctct agagttgaag aacctgtggc ggagagggtg
      541 ggattcgaac ccacggtacg gttgcccgta caccgcattt cgagtgcggc gcattcgacc
      601 actctgccac ctctccgcga agtgccggta agcgggccat cggccctctg gtcgggagcc
      661 gcgcggttag cggaaggaat ccggcttgcc aagcccctcg ccactcccga cgccaatttc
      721 ctgcgccttt tggtcctcgc cctgcggcgg gaccggtcgc tcctgcttgc ccctgctgcg
      781 gccagcccgg tcgggacatg gcgaaacatc tcgacctgta ccaaattcga catattataa
      841 ttacttatgt tcgtttattc agggctggta tgaaaataaa tcagcacgat cactgaccat
      901 gaggtcgtgc gtgtccaccc cgtgaatgcc gggcaaatca tcgtaatcca cagcataggc
      961 gcggttatcc actatgcttg ccttatggcc ggaaggaact ctgatacagc cgccccagga
     1021 gggtgaatcg gcggattgtc tgccggcttc ccctgtccca gggagcccgc gccgcagctg
     1081 tgccgacgaa cccatccccg gcaggtcacc ggctcccgcg gccaggcggg agcaccttgc
     1141 gtgcgtggtt cgtccgtgcc gtcgccctga tggctgcgct tgcggccccg gccgtgcggg
     1201 ccgacgtgct cgagatcggc ccctccggcg ccagctggat cgccggcggc cccgcgcccg
     1261 acaccggccc ggcggtcgag ggctctaccg ccgcccccgc ctatacccag gtcgccgcca
     1321 ccgctgggcc cgatgcatgg cgcgcccacg tcgccgtcct tgccgaacgc tacgatctca
     1381 gcccggccct gctcgacgcc gtggtctggc aggaaagccg ctggaacccg cgggccgtct
     1441 cgccagccgg cgcccggggc ctggcccagc tcatgcccgc aaccgggcgc cagctggggg
     1501 tggactggcg cgatccgctg gccaacctcg aagggggcgc ccgctacctg cgccagcagc
     1561 tcgaccggtt cggcggcgac gtcgaaaagg cgctcgccgc ctacaatgcc ggcccggccc
     1621 gcgtggcccg cgctggcagc gtgccgccga tccgtgaaac ccaggcctat gtcgcgtcga
     1681 tcctggcgcg cctttcgcaa cccgtccgga gatagccgat gcgtgccgcg cgcctccttg
     1741 ccctcatccc tgccctcgtg cccggcaccg ccatggccca ggccgtggcc gccaatccgc
     1801 aaggctccgg gccgatcgtc gccgccctcg gctggctgca aggcacgctg ctgggcaatg
     1861 tcgccacggc ggtcgccgtc atggccgtcg ccgccgtcgg cttcatgatg ctcacggggc
     1921 ggatgaactg gcgcttcggg gcgaccgtga tcatcggcac cttcatcctg ttcggcgcgg
     1981 gggcgatcgt ctcgggcatc cagtccgtcg cggtgggcgg gtgagcgtaa tgagccctgc
     2041 ccccacccct gccctcgccc gctttcccgt ccaccgcgcg ctcacctggc cgcagatgtt
     2101 cgcgggcgtc acctacaact tcttcatcat taacgccgcc ctcaccaccg aggccttcct
     2161 gatcacccgg agctttctcg ccctgccgct ggcgctgctg atccacacga tcggctattt
     2221 cgcctgcctg cgcgagccgc gggtgttcga cctgtggttg accaaggtca gccgctgccc
     2281 gcgcgtgccc aactggcgcc gctggggctg caacagctac gcgccgtgag cggaatcggc
     2341 ccgagcagct gggcgcgccg cgaagcccac gccggcgacc gcctgcccta ccgcgccctg
     2401 cttga
//
LOCUS       DATNBV010000448         7153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1538194,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000448 DATNBV010000000
VERSION     DATNBV010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538194"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VN627_10600"
     CDS             complement(<1..432)
                     /locus_tag="VN627_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HXD03745.1"
                     /translation="MTLRHLPFALVLAAGAAAPALAERPPRPLAEARLVDASGKEVGS
                     ARFVTGKDGVVLEVTARGLTPGPHGMHLHAVGQCDGPDFTSAGPHLNPDGHEHGDDNP
                     QGSHLGDLPNVTAGRDGVVYAKIRTPLRTFKLMKNVLDGDGT"
     gene            complement(450..1328)
                     /gene="hemF"
                     /locus_tag="VN627_10605"
     CDS             complement(450..1328)
                     /gene="hemF"
                     /locus_tag="VN627_10605"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HXD03746.1"
                     /translation="MNDWTPYTERARTWFESLRDMICAEFEAIEREAGSDARFAYTPW
                     SRDSVGGADATNGNGETGGGVQGLMKGKVFEKVGVNVSTVRGAFSPQFAASINGADPE
                     NPQFSATGISLVAHIANPQVPAVHMNTRFLTTTRAWFGGGADLNPPLPKAEDTAEFHA
                     AMQGACAPHGADYYAKFRAWADDYFYIPHRKVHRGVGGIFYDHLECADAQAWEANFAF
                     TQDVGRAFLEVFPRIVRRRMGEAFTPEDKRTQLEYRGRYAEFNLVYDRGTLFGLKTGG
                     NIDAILMSLPPEAAWS"
     gene            1375..2055
                     /gene="pdeM"
                     /locus_tag="VN627_10610"
     CDS             1375..2055
                     /gene="pdeM"
                     /locus_tag="VN627_10610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HXD03747.1"
                     /translation="MVPLSFAGQEWRLTGSSALGGALYWPRENALLVADLHLEKASFF
                     AATGQPLPPYDSRETLERIALALRETGARRVYCLGDSFHDAAGPVRLEPHAAGMLAAL
                     TRVADWVWITGNHDASAALPGTTAGELAVSGVILRHEAKPGEIAPELSGHFHPKLRVT
                     VRGRRIARPCAVRSERRMILPAFGALTGGMNAADPAILKALQPAAAIDAVLPAAGRLV
                     QYPLWRAA"
     gene            complement(2060..2491)
                     /locus_tag="VN627_10615"
     CDS             complement(2060..2491)
                     /locus_tag="VN627_10615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HXD03748.1"
                     /translation="MTIKRTLLAALALAGAGGALPAPGHAQPASEEITVVGRYGRVPD
                     SVQSLSQAVSYADLDLSTQAGRDILRHRVALTARYLCDKMGETDTSSGPVVPSCRDAA
                     VSDAMNRVGTIEAHFAPRGSTWVAGTAWAPPYPADWDRRYP"
     gene            complement(2601..3932)
                     /gene="gltX"
                     /locus_tag="VN627_10620"
     CDS             complement(2601..3932)
                     /gene="gltX"
                     /locus_tag="VN627_10620"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXD03749.1"
                     /translation="MVTVTRFAPSPTGTLHVGNIRTALHNWLLARKDGGTFLLRIDDT
                     DAERSREEYVEAIRADLAWLGMVPDGEERQSARTAIYEQEFARLVEAGRVYRCYETAQ
                     ELDLKRKVLLGRGLPPIYDRAALALTEADHAAKAAAGERPHWRFMLDRETPIEWVDGI
                     RGPQQFDPASMSDPVVRRADGSWLYMLPSVIDDVAMGVTQVLRGEDHVSNTAAQVQMF
                     TALGASPPAFAHEALLVGSEGKLSKRLGSLGVAAFRDEGIEPEALVALLARLGTSQPV
                     EPIVDRGELVASFDLGTFGRAPARFDEAELHRLNAQIVHQLPFDRVAHLLPSGMSETA
                     WAAIRPNLAHIAEAADWWQVVTGPVAAPQLADEDGAFVREAGEVLAGLDWGEGVWAGL
                     TSALKERTGRKGKALFLPLRLALTGREHGPDMAALLPLIGREAALQRLSRA"
     gene            complement(3965..4204)
                     /locus_tag="VN627_10625"
     CDS             complement(3965..4204)
                     /locus_tag="VN627_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03750.1"
                     /translation="MDRVSRLKGGGYLTSTLSVILLGIPAMKSAMESPPMLASLLAGM
                     GLSVAGMALRWHSHRLNSRKETRERDALRRDVRAA"
     gene            complement(4356..6011)
                     /locus_tag="VN627_10630"
     CDS             complement(4356..6011)
                     /locus_tag="VN627_10630"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HXD03751.1"
                     /translation="MVDSLKITLAQMNQTVGDLAANAAAMLAVRERARGADLVVFPEL
                     QLIGYPPEDLILKPALVERAAAELEKLAAATADGGPAMLVGSVFVRDGALHNGVALLD
                     GGKIAAVRFKHELPNYGTFDEMRLFQPGDLPEPIVFKGVMIGVPICEDIWQPDVCRHL
                     ADFGAELLIVPNGSPYEIDKDALRIDGVAKLRAVDTRLPLAYLNRVGGQDELVFDGSS
                     FVINGDGALAVQMKDWEEQVLDTRWTRTATGWRCDRGEIAQLADHPEDIYCAMVMALR
                     DYVDRNRFPGVVLGLSGGIDSAICAAIAADALGPERVWCVMLPSAYTSQESLDDATEC
                     ARLIGCRYDTVPIAPAVDAFTEMLRPLFTGKAPDLTEENLQSRIRGVTLMGLSNKFGP
                     MLLTTGNKSEMSVGYATIYGDMAGGYNPLKDAYKMTVFAISRWRNTHKPKIGLGPDGP
                     VIPENIITKPPSAELRPDQKDSDSLPPYEVLDAILLGLVEHEKSVAQLVGEGFDHDTV
                     VRIERLLHLAEYKRRQAPPGVKLGSRNFGRDRRYPITHSFRSA"
     gene            complement(6076..>7153)
                     /locus_tag="VN627_10635"
     CDS             complement(6076..>7153)
                     /locus_tag="VN627_10635"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavin-dependent monooxygenase"
                     /protein_id="HXD03752.1"
                     /translation="DIPEATIAAMKAAGLLRVYQPARWGGLECDPRDVYDIQNLFAET
                     CLSTGWVYGVLALQSFFLARFGEQAQADVWGKNPDALLSSSFAPLGKVTRVEGGFRVS
                     GRYGYSSGSSHADWAVVGGIVAPDGEGDVPHLRLFLIPRADYVIDDMWHTIGLRGTGS
                     NDLVIADAFVPDHRIYRPDPGLVPLPAGSGLAPLHRLPWVHMASATIGNVAIGGTRAA
                     VRAFADATRARRAAPPPPGVPENPALLSVIARAEAEADATGAVSRRNIGRYFSAIAEN
                     RAMEPAEALTYRVQITGSLRRLTALVDEMVLLSGGHAIREGSPLTRIWLDLIAARAHF
                     GNDPAGAMAMLGSTLDAGFAQETV"
BASE COUNT         1129 a         2611 c         2304 g         1109 t
ORIGIN      
        1 ggtgccgtcg ccatcgagca cgttcttcat cagcttgaaa gtccgcagcg gcgtgcggat
       61 cttggcgtag acgaccccgt cccggcccgc ggtcacgttg ggcaggtcgc cgaggtggct
      121 gccctgcgga ttatcgtcac cgtgctcatg gccatcggga ttgaggtgcg gccccgcgct
      181 ggtgaagtcc ggcccgtcgc actggcccac cgcatggagg tgcatcccat gcggccccgg
      241 cgtcagcccg cgcgcggtca cttccagcac caccccgtcc ttgccagtga cgaaccgggc
      301 cgagcccacc tccttgcccg aggcgtcgac cagccgcgcc tcggccagcg ggcgcggcgg
      361 gcgctcggcc agcgctggcg cggctgctcc agcggcgagt acaagggcaa aaggcaggtg
      421 gcgcagggtc atcggaaagg ctccgcaggt caggaccaag ctgcctcggg cggcaggctc
      481 atcaggatcg cgtcgatatt gcctccggtc ttcagcccga acaaggtccc ccggtcgtag
      541 accaggttga actctgcgta acggccccgg tattccagct gggtgcgctt gtcctccggc
      601 gtgaaggcct cgcccattcg ccgccgcacg atccggggaa acacctcgag gaaggcccgg
      661 ccgacgtcct gggtgaaggc gaagttcgcc tcccaggcct gcgcgtcggc gcattcgagg
      721 tgatcgtaga agatcccgcc cacgccgcga tgcaccttgc ggtgggggat gtagaaatag
      781 tcgtcggccc aagccctaaa cttcgcatag tagtccgccc cgtgcggagc gcaggccccc
      841 tgcatcgccg cgtggaactc ggccgtatcc tcggccttgg ggagcggcgg gttaaggtcg
      901 gcgccgccgc cgaaccaagc cctagtcgtg gtcaggaacc gcgtattcat gtgcacggcg
      961 ggcacttgcg gattggctat gtgggccacc aggctgatgc cggtggcgct gaactgcggg
     1021 ttctcgggat cggccccgtt gatcgaggcg gcgaactgcg ggctgaacgc gccgcgcacg
     1081 gtggagacgt tgacgcccac cttctcgaac accttgccct tcatcagccc ctgcacgccg
     1141 ccgccggtct cgccattccc gttcgtggca tcggccccgc caacgctgtc gcgcgaccac
     1201 ggggtatagg caaagcgcgc gtcgctgccc gcctcgcgct cgatcgcctc gaactcggcg
     1261 cagatcatgt cgcgcaagct ttcgaaccag gtccgcgcgc gttcggtata gggggtccag
     1321 tcattcatcg cagagcccct tgccaagccc cgcccgctgc cgcaaggaaa agccatggtt
     1381 cccctttcgt tcgcaggtca ggaatggcgg ctcaccggct cctcggcctt ggggggcgcg
     1441 ctctactggc cgcgtgagaa tgccctgctg gtggccgacc tgcatctcga gaaggccagc
     1501 ttctttgccg ccaccggcca gccgctcccg ccctatgaca gccgcgagac gctggaacgc
     1561 atcgcgcttg ccctgcgcga aacgggcgcg cggcgggtct attgcctcgg cgattcgttc
     1621 cacgatgccg ctggccccgt tcggctcgaa ccccacgccg ccggcatgct cgcggcgctc
     1681 acccgcgtgg cggactgggt gtggatcacg ggcaatcacg atgccagcgc cgccctgccc
     1741 ggcaccaccg cgggcgaact cgccgtctcg ggcgtgatcc ttcggcacga ggcgaaaccg
     1801 ggcgaaatcg cgcccgaact gtccggacat ttccacccca agctgcgcgt caccgttcgc
     1861 ggacggcgca ttgcccggcc ctgcgccgtc cgctcggaac ggcggatgat cctaccggcc
     1921 tttggcgcgc tcaccggcgg catgaacgcc gccgatccgg cgatcctcaa ggcgctccag
     1981 ccggccgccg ccatcgatgc cgtgctcccg gcggcgggcc ggctggtgca gtacccgcta
     2041 tggcgggctg cctagccgat tacgggtaac ggcggtccca atccgctgga tagggcggcg
     2101 cccaagccgt ccccgccacc caggtgctgc cgcgcggcgc aaagtgggcc tcgatcgttc
     2161 cgacccggtt catggcgtcg ctcaccgcgg catcacggca cgaaggaacg acggggcccg
     2221 agctcgtgtc ggtctcgccc atcttgtcgc acaggtagcg cgcggtcagg gcgacgcgat
     2281 ggcgcaggat atcgcggccc gcctgggtcg aaagatcaag gtcggcatag ctcacggcct
     2341 ggctcagcga ctgcacgctg tccggcacgc ggccatagcg gccgaccacg gtaatttcct
     2401 cggatgccgg ctgggcgtgg cccggcgcag gcagggcgcc accggcgccg gcaagggcaa
     2461 gggctgctag caaggtgcgt ttgatcgtca tcaggcttct ccgagcaagt catcgtagcc
     2521 cgtccgccaa gttgacgatt ccccgccccc cgccgttccg cttttcccga cgagccgtta
     2581 gccagccgcg acgactgccc tcaggcgcgg gatagccgct gcagcgccgc ctcgcgcccg
     2641 atcagcggca gcagcgcggc catgtcggga ccgtgctcgc ggcccgtaag cgcgagccgc
     2701 agcggcagga acagcgcctt gcccttgcgc cccgtccgct ccttcaacgc cgaggtcagc
     2761 ccggcccaga caccctcgcc ccaatccagc ccggccagca cctcgcccgc ctcgcgaaca
     2821 aaagcgccat cctcgtcagc cagctgcggc gccgccaccg ggccggttac cacctgccac
     2881 cagtccgctg cctcggcgat gtgggcaagg ttcggccgga tcgccgccca ggccgtttcg
     2941 ctcattcccg agggcaacag atgcgctacc cggtcaaatg gcagctggtg cacgatctgg
     3001 gcgttcagcc ggtgcagctc ggcctcgtcg aaccgtgccg gggcccgccc gaacgtgccc
     3061 agatcgaagc tcgcgaccag ctcgccccgg tcaacgatcg gctccaccgg ctgcgacgtg
     3121 ccgagccgcg ccagcagggc cacgagcgcc tcgggctcga tcccctcgtc acggaaggcc
     3181 gccacgccca gcgagccgag ccgcttcgac agcttgccct cgctccccac cagcagcgcc
     3241 tcgtgggcaa aggccggcgg gcttgctccc aacgcggtga acatctgcac ctgtgccgcc
     3301 gtattgctga catggtcctc gccgcgcagc acctgggtga cgcccatggc gacatcgtcg
     3361 attaccgaag gcagcatata gagccacgag ccgtccgccc gccgcaccac cggatcgctc
     3421 atgctggccg gatcgaactg ctggggcccg cgaatgccgt cgacccattc gatcggcgtc
     3481 tcccggtcca gcatgaaccg ccagtgcggc cgctcccccg ccgcagcctt ggccgcatgg
     3541 tcggcctcgg tcagcgccag ggccgcccgg tcatagatcg gcggcaaccc gcgcccgagc
     3601 aggaccttgc gcttgaggtc cagctcctgc gcggtctcat agcagcgata gacgcggccc
     3661 gcttccacca gccgcgcgaa ctcctgctca tagatcgccg tgcgggccga ctgccgctcc
     3721 tcgccatcgg gcaccatgcc cagccaggca aggtccgcgc ggatcgcctc gacatattcc
     3781 tcgcggctgc gctccgcatc ggtatcgtcg atccggagca ggaaggtccc gccgtctttc
     3841 cgcgccagga gccagttgtg cagcgccgtg cggatattgc cgacgtgcag cgtgccggtg
     3901 ggcgaagggg caaagcgggt cacggtaacc atggccgcgc cctaccccgc ccaggcctcc
     3961 aaggctaggc cgcccggaca tcccggcgca gcgcatcccg ctcgcgcgtc tccttgcggc
     4021 tgttcagccg gtgcgaatgc cagcgcagcg ccatgccggc gaccgacagg cccatccccg
     4081 ccaacaggct ggccagcatc ggcgggctct ccatggccga tttcatcgcg ggaatgccca
     4141 gcaggatcac gctgagggtt gaagtgagat agccgccgcc cttgagccgc gaaaccctgt
     4201 ccattggcgc caagtcctcc atgccgcaac aagggcaatg atccaccgcc gccgagggtt
     4261 cccctcaccc gccgcgccac aagcccccgt caccaaccgc cgtcatcctg aaacgggttc
     4321 aggatgacga acctcgcacg agcgggggaa gccccttacg cgctgcggaa cgaatgggtg
     4381 atcggatagc gccggtcgcg cccgaagttg cggctcccca gcttcactcc cggcggagcc
     4441 tggcgccgct tgtattcggc caggtgcagc agccgctcga tccgcaccac cgtgtcgtga
     4501 tcgaacccct cgcccaccag ctgggccacg cttttctcgt gctccaccaa cccgagcagg
     4561 atcgcgtcga gcacctcgta gggcggcagc gagtccgaat ccttctggtc cggccgcagt
     4621 tccgcgctcg gcggcttggt gatgatattc tccgggatga ccggcccatc cggccccagc
     4681 ccgatcttgg gcttgtgcgt gttgcgccac cggctgatgg cgaatacggt catcttgtag
     4741 gcgtctttca gcggattgta gccgcccgcc atgtcgccgt agatcgtggc ataacctact
     4801 gacatctcgc tcttgttgcc cgtcgtcagc aacatcggcc cgaacttgtt ggacaggccc
     4861 atcagggtca cgccgcggat gcgcgactgc aggttttcct cggtcaggtc cggcgccttg
     4921 cccgtgaaca ggggacgcag catttcggta aaggcatcca ccgccggggc gatcggcacg
     4981 gtatcatagc ggcacccgat cagccgggcg cactcggtcg catcgtcgag gctctcctgg
     5041 ctggtgtagg cgctcggcag catgacgcac caaacccgct ccggccccag cgcgtcggcc
     5101 gcgatggcgg cgcaaatcgc agagtcgatc ccgcccgaaa gccccagcac cacgcccggg
     5161 aagcggttgc ggtcgacata gtcgcgcagc gccatgacca tggcgcagta gatgtcctcc
     5221 gggtggtcgg cgagctgggc gatctcgccc cggtcgcagc gccatcccgt cgccgtgcgg
     5281 gtccagcggg tgtcgagcac ctgctcttcc cagtccttca tctgcacggc cagcgcgccg
     5341 tcgccgttga tgacgaagct cgacccgtcg aacaccagtt cgtcctggcc gcccacccgg
     5401 ttgagatagg cgagcggcag ccgggtatcg acagcgcgca gcttggccac gccatcgatc
     5461 ctgagcgcat ccttgtcgat ttcgtagggg ctgccattcg gcacgatcag cagttccgcg
     5521 ccgaaatcgg ccaggtgccg gcatacgtcg ggctgccaga tgtcctcgca gatcggcacc
     5581 ccgatcatca cgcccttgaa cacgatcggc tcgggcagat cgcccggctg gaacaggcgc
     5641 atttcgtcaa aagtgccgta gttcggcagc tcgtgcttga agcgcaccgc cgcgatcttg
     5701 ccgccgtcga gcagggccac gccgttgtgc agcgccccgt cgcgcacgaa tacgctcccc
     5761 accagcatcg ccgggccgcc gtccgccgtc gccgccgcaa gcttttcgag ctccgccgcg
     5821 gcacgttcga ccagcgccgg cttgaggatc aggtcctccg gcgggtagcc gatcagctgc
     5881 aactcgggga acacgacgag gtccgccccc cgcgcccgtt cgcgcacggc cagcatggcc
     5941 gcggcgttgg cggcaaggtc accgaccgtc tggttcatct gcgcgagggt gatcttgaga
     6001 ctgtcgacca tgagccctga ctaggatggg atgcgtctca agggaatagg gcgcgagact
     6061 tcgcgttccc gtaccctaga ccgtttcctg cgcgaaccct gcgtccagcg tggagcccag
     6121 catggccatc gccccagccg gatcattgcc gaaatgggcg cgggccgcga tcaggtcgag
     6181 ccagatgcgc gtcagcggtg atccctcgcg gatggcgtgg ccgccactca gcaggaccat
     6241 ttcatccacc agagcggtca gccggcgcag cgatccggtt atctggacgc ggtaggtcag
     6301 cgcctcggcc ggctccatcg cccggttctc ggcgatggcc gaaaagtacc ggccgatgtt
     6361 gcgccgcgac acggccccgg tggcatcggc ttccgcctcg gcccgcgcga tgacggaaag
     6421 cagcgccggg ttctcgggca cgcccggggg cggcggcgct gcccggcggg cccgcgtagc
     6481 gtcggcaaag gctcgcacgg cagcgcgcgt gccgccgatc gccacattgc cgatggtggc
     6541 cgacgccatg tgcacccacg gcagccggtg aagcggcgcc agaccgcttc ccgccggcaa
     6601 gggcacgagg ccggggtcag gacgatagat ccggtgatcg ggcacaaagg catcggcgat
     6661 caccaggtcg ttgctgcccg tgccgcgcag accgatcgtg tgccacatgt catcaatgac
     6721 ataatcggcg cgcggaatca ggaacagccg caagtgcggc acgtcgccct cgccatccgg
     6781 agccacgatc ccgccgacca cagcccaatc ggcatggctg ctgccgctcg aatagccgta
     6841 acggccggag acccgaaaac cgccctcgac ccgcgtcacc ttgccgaggg gcgcgaagga
     6901 ggaggacagc agggcgtcgg ggttcttccc ccagacgtcc gcctgggcct gttcaccgaa
     6961 ccgggccagg aagaacgact gcagcgccag caccccatag acccacccgg tcgagaggca
     7021 ggtctcggca aacaggttct ggatatcgta gacgtcgcgc ggatcgcact ccagcccgcc
     7081 ccagcgcgcc ggctggtaga cgcgaagcag gcccgccgcc ttcatggctg cgatagtcgc
     7141 ctcggggata tcg
//
LOCUS       DATNBV010000449         3166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4724984,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000449 DATNBV010000000
VERSION     DATNBV010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4724984"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="VN627_10640"
     CDS             complement(<1..132)
                     /locus_tag="VN627_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HXD03753.1"
                     /translation="MIRKLLIANRGEIAIRVARTCRRLGIDVASVHSVADRDAMHVRE
                     "
     gene            complement(138..1739)
                     /locus_tag="VN627_10645"
     CDS             complement(138..1739)
                     /locus_tag="VN627_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXD03754.1"
                     /translation="MISSKIDTRSPGFRDNEQHYRALIGELREKRSVARAGGDAAAKA
                     RHVGAGKMLARQRVDALLDAGSPFLEVCTLAGDTPDSSAPLGAGLVTGIGMVSGRACM
                     IIANDATVKGGTYFSQTCRKHVRAQQIAWRHGLPNLTLVDSGGAFLPEQAGIFPDVGQ
                     FGTVFHQIVRQSADGIPQIASVHGACTAGGAYIPALSDQTIIVQQQGYMHLGGPEIVF
                     AATGEQIDRETLGGADMHSRVSGVTDHLAEDDRHALRIMRELVRDLPGQPTFVRPPSA
                     SVPPRYDPAELYGIISKDPRIPTNTREILARIVDDSDFDDFKPLYGSTLLCGFARING
                     FEVGILANQGVLFTECALKAVHFIDLCCKRNIPLLFMADITGYMVGRDAETGGISKAG
                     AKMITAMSSANVPKYNIIIGNSFGAGYMGMCSRPFEPTLAFGWPNGRAALMGPDQAAT
                     TLAMVQRAKRARDGIEWPADEEAAFRAQIAGEFDAFQNIYNFSSHIWLDDIIDPVETR
                     AVMGLALDLAARTTPPETRFGVLRM"
     gene            2046..2666
                     /locus_tag="VN627_10650"
     CDS             2046..2666
                     /locus_tag="VN627_10650"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXD03755.1"
                     /translation="MSDERSTKQRILDMAAASFRTDGLAFTSMADLAKITGVKTSSIY
                     YYFPSKDALIEETFRIGIELVHSKVQEALESVGPGATHRERFNVAFAAHLSALLGEND
                     YSSANIKNYSHASVEVRKKNREVRAKYGRLWMSLLKDAQADGAIGDDVNLSMVRMLLI
                     GAMNWATEWYSPKGGTIPEIAECLCRMLDGLAPRPAGAAAVSHQPA"
     gene            2846..>3166
                     /locus_tag="VN627_10655"
     CDS             2846..>3166
                     /locus_tag="VN627_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03756.1"
                     /translation="MDQWQLSGKVALVTGASSGLGAHFAATLAAAGAHVILAARRMDA
                     MAPVASAIAAAGGTCETLGLDVSDPASIAAARAALARTDILVNNAGVVREAPVLDQSE
                     ADWDV"
BASE COUNT          569 a          989 c         1043 g          565 t
ORIGIN      
        1 ctcgcgcacg tgcattgcgt cacggtcggc cactgagtgg accgaggcga catcgatgcc
       61 caagcggcgg caggtccggg cgacccggat cgcgatctcg ccacgattgg cgataaggag
      121 tttgcggatc atcacggtca cattctcagc acgccaaagc gcgtttcggg cggcgttgtc
      181 cgggcggcga gatcaagcgc caggcccatg accgcccggg tttcgacggg gtcgatgatg
      241 tcatcgagcc agatgtgcga cgagaagtta tagatgttct ggaacgcatc gaactcgccg
      301 gcgatctggg cgcggaacgc cgcctcctcg tccgccggcc attcgatccc gtcgcgggcg
      361 cgcttggccc gctggaccat ggccagggtc gtcgcggcct gatccggtcc catcagcgct
      421 gcccggccat tcggccagcc gaacgccagc gtcggctcga acggccggct gcacatcccc
      481 atgtagccag caccgaagga gttgccgatt atgatgttgt acttgggcac gtttgcagac
      541 gacatggccg tgatcatctt cgccccggcc ttggaaatgc cgccggtctc cgcgtcgcgg
      601 cccaccatgt agcctgtgat gtcggccatg aacaggagtg ggatgttgcg cttgcagcac
      661 aggtcgatga agtgcaccgc cttgagtgcg cactcggtga acagcacccc ctggttggcg
      721 agaatgccca cctcaaaccc gttgatccgc gcgaagccgc aaagcagcgt gctgccatag
      781 agcggcttga aatcgtcaaa gtcgctgtcg tccacgatcc gcgccaggat ctcgcgcgtg
      841 ttcgtcggga tgcgcgggtc cttgctgata atgccgtaga gttcggccgg atcatacctt
      901 ggcggaaccg aggccgaagg cgggcgcacg aacgtgggct gtcccggcag gtcgcgcacc
      961 agttcgcgca tgatcctcag cgcgtggcga tcgtcttccg caaggtggtc ggtgaccccg
     1021 ctgacgcgcg aatgcatgtc ggcgccgccg agcgtttcgc gatcgatctg ctcgcccgtc
     1081 gccgcgaaga cgatctccgg tccgccaagg tgcatgtagc cttgctgctg gacgatgatc
     1141 gtctggtccg acaaggccgg gatataggcg ccgccggccg tgcaagcccc gtgcacgctg
     1201 gcgatctgcg ggatcccgtc ggccgactgg cgcacgatct ggtgaaacac cgtgccgaac
     1261 tgcccgacat cggggaagat gccggcctgc tcgggcagaa aggctccgcc tgaatccacc
     1321 agcgtcaggt tgggaaggcc atggcgccat gcgatctgct gggcccggac gtgcttgcgg
     1381 caggtctggc tgaagtaggt cccgcccttc accgtcgcgt cgttggcaat gatcatgcag
     1441 gcgcgcccgc tgaccatgcc gatcccggtg accagccccg cccccagcgg ggcgctcgaa
     1501 tccggcgtat cgccggccag tgtgcagacc tcaaggaacg gcgaccccgc gtccaggagc
     1561 gcatcgacgc gctgccgggc cagcatcttg cccgcgccga catgccgcgc cttggcagcg
     1621 gcgtcaccgc ctgcacgagc aacactgcgc ttctcccgca gttcgccgat cagcgcgcgg
     1681 taatgctgtt cgttgtcccg gaagccaggt gaccgggtgt caattttgct ggaaatcacc
     1741 ggcacgacaa tctctccacg ccatctccgc cgataacaca ctgattggcg ccaatccgtg
     1801 caagcgcaga ggagcccgac ccctcgccga ggctccctcg atgcgcatgc tagccccgag
     1861 gtcctgaacg atcggtagat agagatcgca tcgataacaa ggaaaaacga aacggtgctg
     1921 atcggggctg cacaggccgg ctcggcattt gccttgccct ttggcgggtg caggactaag
     1981 caggcgggga gcgcgtgcgt ttttggcact gtggctctgg tgcaaaaggc tagaaacttc
     2041 ctgaaatgag tgatgaaaga agcaccaagc agcgcattct agacatggct gccgcctcat
     2101 ttcgaacaga tggtctcgcg ttcacgagca tggcggatct ggcgaagatc accggcgtga
     2161 agaccagcag tatctattat tattttccgt cgaaggacgc cctgatcgag gagacgttcc
     2221 gcatcgggat cgagctcgtt catagcaagg tgcaggaagc gctcgaaagc gtcggccctg
     2281 gtgccacgca ccgcgaacgg ttcaatgttg cgttcgccgc ccatctgtcg gcattgctgg
     2341 gcgagaatga ttattcgtcg gcaaatatca agaactactc ccatgcgtcg gtcgaggtca
     2401 ggaagaaaaa tcgggaggtc cgggccaagt atgggcggtt atggatgtcg ctcctcaagg
     2461 acgcccaggc tgacggtgcg attggcgatg atgtaaacct ctcgatggtg cggatgctgc
     2521 tcatcggtgc catgaactgg gcgactgagt ggtattcccc gaagggggga accatcccgg
     2581 agatagccga atgcctctgc cgcatgcttg acggcctggc accccggccc gccggcgccg
     2641 cggccgtgag ccatcagccg gcctgatcgg caagcaactg gcccaaacca ctggcatgca
     2701 gtgcccgccg atgccggaca tctgcctggc ggggagcgaa cggccgttgc cggtgtcgag
     2761 atggagacgg gtgggttcgg gttgacccgg tcgcttctga acccgggcgc catccggtgc
     2821 ggcctgataa tgtgagtgaa acgcgatgga tcagtggcaa ttgagcggca aggtcgcact
     2881 tgtgacggga gcctcgtcgg ggctgggtgc acattttgcg gctacgcttg ccgcggctgg
     2941 ggcccacgtc atcctggcgg ctcgccggat ggatgcgatg gcgccggtgg ccagtgcgat
     3001 cgcggctgcc ggcggcacgt gcgaaacgct gggcctggat gtctccgacc ccgccagcat
     3061 tgccgcagcc cgagcggcgc tggcccggac cgacattctc gtgaacaacg ccggcgtggt
     3121 gcgcgaggcc cctgtgctcg accagagcga agccgattgg gacgtg
//
LOCUS       DATNBV010000450         5992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3071852,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000450 DATNBV010000000
VERSION     DATNBV010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3071852"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VN627_10660"
     CDS             <1..201
                     /locus_tag="VN627_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03757.1"
                     /translation="GKSMGWLQARLKAYDPQARFYPEGDHLHVSFPGYYGAPAIGGAR
                     KAGLRNPLMGMPAPPPGFRLDR"
     gene            319..1257
                     /locus_tag="VN627_10665"
     CDS             319..1257
                     /locus_tag="VN627_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03758.1"
                     /translation="MGRFLKIDRAVDRFGYWVGAYTIMSGGFAWLASKMEALHIGWAE
                     AVFIGVFVAGGVTLVVSTGLVAWRQFHPLPGQSSPDSTELERLIDARLQARTDLATHA
                     DLHRHDEQLVELSERTTFVRKMVESGHSGLKARLDEIAKGCSDLEQRWNEWTRQHSND
                     QEARFDNVDFGFAAIINREWNERLFAELREGFVEIAEPVEAKKPIADAGRWQMLVTNW
                     RSKLDQWLVIADYYAVNTRENVLIVPDHLYYGTWSLAEDRLTADQVKMYKEAAIWWMS
                     AKAAKPRVDRCLAAAAFESPSRKGRSGSPPRIGSNG"
     gene            complement(1323..1691)
                     /locus_tag="VN627_10670"
     CDS             complement(1323..1691)
                     /locus_tag="VN627_10670"
                     /inference="COORDINATES: protein motif:HMM:NF022172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HXD03759.1"
                     /translation="MKLDQDLVRQILLGIEASDESPQHWVSFDLPGTPRETVSYHIQL
                     LHEAGLIVGQDTSSLSDYRWEAKRLTYSGHEFLDTIRDSGVWRATKSAAAKAGGASIS
                     FLWEIAKAELRGRLGLPPGQ"
     gene            complement(1783..1859)
                     /locus_tag="VN627_10675"
     tRNA            complement(1783..1859)
                     /locus_tag="VN627_10675"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1823..1825),aa:Pro,seq:tgg)
     gene            complement(1938..2013)
                     /locus_tag="VN627_10680"
     tRNA            complement(1938..2013)
                     /locus_tag="VN627_10680"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1978..1980),aa:Ile,seq:cat)
     gene            complement(2094..3104)
                     /locus_tag="VN627_10685"
     CDS             complement(2094..3104)
                     /locus_tag="VN627_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXD03760.1"
                     /translation="MAEALITDPPREDRFVRFAPDFGQRFLITVDTEEEFDWDKPLRA
                     EGHTLNSVSRLMRFQQFCEGHGVVPVYLVDYPVATSPLAGDLLRDAVAAGRAEVGVQL
                     HPWVSPPFGEQISEFNSFAGNLPADLERAKFARLRATIEERFGASPLIYRAGRYGVGP
                     NTPGVLRDLGIAIDSSVRARFDYSSGGGPNFRDLPVRPWWVDRPGGLMELPLTTVYWG
                     GLARAGAGHWLYPRLWRVPKLRGALARVGLLERIPLTPEGVNATEARRGIATAVRQGL
                     PVLVFSFHSPSLQPGHTPYVRSEDELDAFYDWWRAAFAQLDRLGVRPTTVSGIMAATQ
                     LS"
     gene            complement(3127..4905)
                     /locus_tag="VN627_10690"
     CDS             complement(3127..4905)
                     /locus_tag="VN627_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXD03761.1"
                     /translation="MHVDDRLATVLGARVDSIVGRRIQYRQLIDIVGTAGAGARSEGL
                     DAAYVRLGELARELPVEERARALAEPGVRLRNPRLVAELAADAPPVAVAAIRAARLDD
                     AQWLDLVPALPLAARGLLRHRRDLGPEVADRLERLGIADRGLPPVDDREIPEVAAQTG
                     AEPAPAPPTAEPESQTPAPPPLAAVPSSPVPLQAAERSLPPAGDGIGALVRRIEAFRR
                     SRQGTAPPLPANDAPRLPLGEREADMPPLAAFAFTTDRTGRIAWTDAPLPGMLVGLSL
                     GLAGAADDRAPEDLATTVRRRLPLRGRRIALVGAAAMAGDWVVDAMPDFDGAGTFTGY
                     LGRCRRPAPPAAAPPTEADRMRQVLHELKTPVNAIQGFAEVIQQQLFGATPHEYRALA
                     AGIAGDAARLLAGFDELDRWARLDAGVLAPEAGTCDFAMVVAGIVHQLEPYTASRSSG
                     FSLVADPGEILIPLDRSEAERLAWRLLATCASFAHPGEALALSLRREDGRVCLVAALP
                     EALAQVTSPAEAPSAPPAQAISAGVFGTAFTFRLLAAEAAGAGGSLVLGGGFARIDLP
                     GAADAGLTAADSAPSHRDGHVSHANS"
     gene            5165..5632
                     /locus_tag="VN627_10695"
     CDS             5165..5632
                     /locus_tag="VN627_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXD03762.1"
                     /translation="MANLDPIDRRLLAELQDDGRMTNVELAGRVGLTAPPCLRRVRAL
                     EDAGVIKGYHADLDGAKLGYAITVFALVSLRSQAEISLREFEEAMKALPEVRECHMLN
                     GEIDFILKIVSRDLQSFQEFLTSKLTPAPNVASVKTSLTIRTAKQEPGVPLEV"
     gene            complement(5655..>5992)
                     /locus_tag="VN627_10700"
     CDS             complement(5655..>5992)
                     /locus_tag="VN627_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXD03763.1"
                     /translation="FRNKRGLASSVWMVNLSPSGCQVQLQFGRLVPGEPVTIRPVGFE
                     NIAGYVRWVKGDRAGIEFTPPLHASVAAHIAKLVVTTSDPRPSFGVADALGRRLLPAP
                     NATITRSVA"
BASE COUNT         1109 a         1914 c         2012 g          957 t
ORIGIN      
        1 ggcaagtcga tgggctggct acaggcgcgg ctgaaggcct acgatccaca ggcgcggttc
       61 tatcccgagg gcgatcacct gcacgtgtcg ttcccggggt actatggcgc tccggcaatt
      121 ggtggtgccc gaaaggcagg cctgcgcaat ccactgatgg gcatgcctgc tccgccgccg
      181 gggttcaggt tggatcggtg acgctgcgcc ctggctgcaa tgcagctatc gcgggattag
      241 cgagctgcaa tcacagcaac acggaaccaa aatggaacag actccacaac ggagtgcgga
      301 tgcggcagga agtcccccat ggggcgattc ctcaagatcg acagggctgt tgaccgtttc
      361 ggctattggg tcggggcgta cacgattatg agtggcggat tcgcttggtt ggcgtccaag
      421 atggaagccc ttcacatcgg ttgggcggaa gcggtcttca ttggcgtgtt cgtcgctgga
      481 ggtgtcactc ttgtcgtaag tacaggactt gtcgcgtggc ggcagttcca tccccttcct
      541 ggccaatcat cgcctgacag caccgagctt gagcggctca tcgatgcacg tcttcaggca
      601 cgaacggacc ttgcgacaca tgcagacctc cataggcacg atgagcagct cgtagagctg
      661 agcgagcgca cgacgttcgt gcgtaaaatg gtcgaatcgg gtcactcagg cttgaaggcc
      721 aggctcgatg agattgccaa aggctgttca gaccttgagc agcgctggaa cgagtggaca
      781 cggcagcaca gcaatgacca agaggcccgc ttcgataacg tcgactttgg gtttgcggcc
      841 attatcaatc gtgaatggaa tgagcgccta tttgcggaac tacgggaggg tttcgttgag
      901 atagctgagc cggtggaggc aaagaagcca atcgctgacg ctggccggtg gcaaatgctt
      961 gtaacgaact ggcgttccaa gcttgatcag tggctcgtca ttgccgatta ctacgcagtg
     1021 aacacgcgcg aaaacgtgct gatagtacca gaccacctgt attatggaac ttggtccctc
     1081 gccgaggaca ggctgactgc cgaccaggtt aagatgtaca aagaagcggc aatttggtgg
     1141 atgagcgcca aggcagcgaa gcctcgcgta gatcggtgcc tggccgcagc ggcatttgag
     1201 agcccatctc gaaagggcag gtcaggtagc ccgccaagga ttggttcaaa cgggtgaccg
     1261 gatggccgct cgtcaccaga tcgccgtttg gcggctattg aaaccggaac aggcatttgg
     1321 ccctattgtc ccgggggcaa tcccagcctg ccacgcagtt cagccttcgc aatttcccac
     1381 agaaagctga tgctggctcc tccagccttc gctgctgctg atttggtggc ccgccagacg
     1441 ccgctgtccc gaatggtgtc caaaaactca tgccccgaat aggtcagccg cttggcctcc
     1501 cagcggtagt cgctcagcga gctagtatct tggcccacga tcagcccggc ttcgtgtagc
     1561 agttgaatat gatatgagac tgtttcgcgg ggggtgccgg gcaaatcgaa gctcacccaa
     1621 tgttgaggtg attcgtcact tgcctcgatg ccgaggagga tctgacggac caagtcctgg
     1681 tcgagtttca tcagaccccg tgatagctag gtgattgctg gaggcatttt cgggtaggct
     1741 cttggcacct cggataacat caaaaaacgg cagaaaagcc agtggtcggg gagacaggat
     1801 tcgaacctgc gaccctctgc tcccaaagca gatgcgctac caggctgcgc tactccccga
     1861 cgcgccggcg ctagacgctg ccggcccggt aggcaagagc gcaaggacag tgcgggaggc
     1921 gcaagggaag ggaagggtgg tgggcccggc aggattcgaa cccgcgacct agccgttatg
     1981 agcggccagc tctaaccgct gagctacagg ccccaaccat cgcggttgca accggcgcgc
     2041 ggtaaccgcg ccaggtgaat taggcaagcg cgcctgtcac ggcgattgtc aggtcatgac
     2101 aattgcgttg ccgccatgat cccgctcacc gttgtcggcc gaacgccgag ccggtccagc
     2161 tgggcaaagg cggcgcgcca ccagtcatag aaggcgtcga gttcgtcttc gctgcggaca
     2221 tagggcgtgt ggccgggctg gagcgagggg ctgtgaaacg agaagaccag caccgggagc
     2281 ccctgccgca cggccgtcgc gatgccgcgc cgggcttcgg tggcgttcac gccctcgggc
     2341 gtcaacggga tccgttcgag caagcccacc cgcgccagcg caccgcgcag cttgggcact
     2401 cgccacaacc ggggatagag ccagtggcct gccccggcgc gggcgagacc gccccagtac
     2461 accgtggtca gcggcagttc catcaagccg cccgggcgat cgacccacca cggacgcacg
     2521 ggcaggtcgc ggaaattggg gccaccaccc gaactgtaat cgaaccgggc gcgaaccgag
     2581 ctgtcgatgg cgatgccgag atcgcggagc acgcccgggg tattgggccc gacgccgtag
     2641 cggcccgccc ggtagatcag cgggctcgca ccgaaccgtt cctcgatcgt tgcgcgcagc
     2701 ctggcgaatt tggcgcgttc gagatcggcc ggcaggttgc cggcaaagct gttgaactcc
     2761 gaaatctgct cgccgaacgg cgggctcacc catggatgca gctggacgcc gacttcggcc
     2821 cggcccgccg ccacggcgtc gcgcagcagg tcccccgcca gcggcgaggt cgccaccgga
     2881 tagtcgacga gatagaccgg gactacgcca tgtccctcgc aaaactgctg gaaccgcatc
     2941 agccgcgata ccgagttgag cgtatgcccc tcggcgcgca gcggcttgtc ccagtcgaac
     3001 tcttcctcgg tatcgacagt gatcagaaaa cgctgcccga aatccggcgc gaaccggacg
     3061 aaccggtcct cccttggtgg gtcggtgatg agcgcttcag ccaattgctg ctccccgccc
     3121 cgcaaatcag gaatttgcgt gcgaaacgtg tccgtcgcga tggctaggtg cgctgtccgc
     3181 cgcggtcaat ccggcatcgg cggcccccgg caggtcgatc cgggcaaagc caccgccgag
     3241 gaccagcgaa ccgccggccc cggcagcttc ggcggcaagc agccggaagg tgaaggccgt
     3301 cccgaacacg cccgcgctga tcgcctgggc cggcggcgcg ctcggcgctt cggccggact
     3361 ggtcacctgg gcaagggctt ccggcaaggc cgcgaccagg cagacccggc cgtcttcgcg
     3421 gcgcagcgac aaggccagtg cctcgcccgg atgggcaaag ctggcgcagg tggcgagcag
     3481 ccgccaggcc agccgctcgg cttcggaccg gtccagggga atcagtattt caccgggatc
     3541 ggccaccagg gaaaagccgc tgctgcggct cgcggtatag ggttcgagct gatggacaat
     3601 gcccgccacg accatggcga agtcgcaggt gcctgcctcc ggtgcgagca cgccggcatc
     3661 cagccgggcc cagcgatcga gctcgtcgaa cccggcgagc aggcgcgcgg catcgccggc
     3721 aatcccggcc gccagcgccc tgtattcgtg cggcgtggcg ccgaacagct gctgctggat
     3781 gacctcggca aagccctgga ttgcgttcac cggggttttc agctcgtgca gcacctgccg
     3841 catccggtca gcctcggtcg gcggcgcggc ggcgggcggg gcggggcggc ggcagcggcc
     3901 gagatagccg gtaaaagtgc ccgcgccatc gaaatcgggc atggcgtcga ccacccaatc
     3961 cccggccatg gccgccgcgc cgaccagcgc gatgcggcga ccgcgcaagg gcaggcgccg
     4021 gcgcacggtc gttgcgaggt cttccggtgc ccgatcgtcc gctgctccgg ccaggccgag
     4081 gctcaggccc acgagcattc ccggcagggg cgcgtcggtc cacgcgatcc gcccggtgcg
     4141 gtcggtagtg aaagcgaagg ccgccagtgg cggcatgtcg gcctcgcgtt cgccgagcgg
     4201 caagcgcggg gcatcgttgg ccggcagcgg cggcgcggtg ccctggcggc tgcggcgaaa
     4261 ggcctcgatc cgccggacaa gggccccgat cccgtctccg gccggcggca gcgaccgttc
     4321 ggcggcctgg agcggcacgg ggctggacgg caccgcggcg agcggcggcg gggcaggagt
     4381 ttgcgattcc ggttcagcgg tgggaggcgc cggcgctggt tcggccccgg tctgcgcggc
     4441 aacctcggga atttcccggt cgtccaccgg cgggagaccg cggtcggcga tgccgaggcg
     4501 ctccagccgg tcggccactt cggggccaag gtcccgccgg tgccgcagaa gaccccgcgc
     4561 ggccagcggc agggcgggaa cgagatcgag ccactgggca tcgtcgagcc gagccgcccg
     4621 gatcgctgcc acggccaccg gcggagcatc ggcagccagc tcggccacga ggcgcggatt
     4681 gcgcagccgc acacctggtt cggcaagggc ccgggcgcgt tcctcgaccg gaagctcgcg
     4741 cgccagttcg ccaagccgga cataggcggc atcgagccct tcgctgcgcg cgcccgcgcc
     4801 ggccgttccg acgatgtcga tcaactgccg gtactggatc cgccgcccga caatgctgtc
     4861 gacccgggca ccgagcacgg tggcaaggcg gtcgtcgacg tgcatcaggt ctccagcagg
     4921 cgggcaaagg gcagggcgcg cagattcggc gttacccatc tataagcgcg aaggacttag
     4981 cagcttgtta cgatccggga agggatcgca ccactattgt cacaatgcgg cacgattgcg
     5041 tttggggaca ataaaattat agtgaaacgc aattaggcca acgaatcttg ccggtctcgc
     5101 tgtgtcggga gctgcgggta gcaggtacgg ccccacggcc aagacaggga aacacacggt
     5161 cgccatggca aatctcgatc cgatcgaccg ccggctgctc gccgaacttc aggacgatgg
     5221 gcgcatgacc aacgtcgaac tcgccggacg ggtcgggctg acggcgccgc cctgcctgcg
     5281 ccgggtccgc gcccttgagg atgcgggcgt catcaagggc taccatgccg acctcgatgg
     5341 cgcgaagctc ggctatgcga tcaccgtctt cgccctcgtc tcgctgcgca gccaggcgga
     5401 aatttcgctg cgcgaattcg aggaggcgat gaaagccctc cccgaagtgc gcgagtgcca
     5461 catgctcaac ggcgagatcg acttcatcct gaagatcgtc agccgcgacc tgcaaagctt
     5521 tcaggaattc ctcaccagca agctgacgcc ggcgcccaac gtcgccagcg tcaagaccag
     5581 cctgaccatt cgcaccgcca agcaggagcc cggcgtgccg ctggaggtgt gaaccgaccc
     5641 ttggggtgga tggctcaggc cacgcttcgg gtgattgttg cattgggggc cggcagcagg
     5701 cgtcggccca gtgcgtctgc aaccccgaaa gacggccgcg gatcgctggt tgtgaccaca
     5761 agcttggcga tgtgcgccgc cacgctcgca tgcaggggcg gagtgaactc aatcccggcc
     5821 cgatcgcctt tgacccacct aacgtagccg gcgatattct cgaatccgac cggacggatc
     5881 gtaacgggtt cccccgggac cagccgtccg aactgcagtt gcacctggca accggatgga
     5941 gacaggttca ccatccagac ggaagaggcg aggccgcgct tgttgcggaa ct
//
LOCUS       DATNBV010000451         3206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_889745,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000451 DATNBV010000000
VERSION     DATNBV010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889745"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(464..2617)
                     /locus_tag="VN627_10705"
     CDS             complement(464..2617)
                     /locus_tag="VN627_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HXD03764.1"
                     /translation="MARSAIRRSLSEQPSAPPPPPPRKQSGGSGGGRRGPGRIGRFFR
                     KLLIWGLALCLAGVLALAAAVMVAASSLPGYQALKSQAQGQMIVVRARDGSELVTMGP
                     SYGKWISISQIPATMKDAMISVEDRRYYDHFGVDPVGILRSLFVRVESGKWRQGGSTI
                     TQQLARNVFLNNNRTFTRKIREAILSIALETKFSKQQILELYLNKVYFGGGAYGIDSA
                     SRKFFGHPGDQLTLPEAAIIAGLVKAPSRYSPTADKAAAVSRAKVVVETMQDAGAITP
                     AEAAAADFTKVTFAQEAGQNSVRYFTDWALPQLDLLLPDNNQPIEVWTTLDPGAQRVA
                     TDAIAKNAPGGAQGALVSMDRDGAILAMVGGTDYVTSNYNRATNAVRQPGSAWKLFVY
                     LAALEAGYKPDDTVVDEPVTIQGWSPHNDARNFAGKIDVRTAFAYSKNTVAAQLGNEV
                     GFGTVAGMARRFGISTPINTMPSMVLGTSDVRLIDMTRAFAAVSARGMAVEPYGISKV
                     TTTDGQVLYEHKPAPGQQLVADYVAAGITDLLQTAVNIGTGRAAQIGRPVAGKTGTTS
                     SNKDGWFIGFSSGITTGVWMGRDDAKPIPGLMGGTAPARAFAQYMRVATARRPVEQFD
                     TALKLPEYTLEPDDEANMGGNPENYYYVDENGNLIDPNRQQGAAVNQSPGRPEDNGQP
                     PAGDGQPPAADDGFLDRATGRQVPPRDAGSGGQGY"
     gene            complement(2730..3128)
                     /gene="msrB"
                     /locus_tag="VN627_10710"
     CDS             complement(2730..3128)
                     /gene="msrB"
                     /locus_tag="VN627_10710"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXD03765.1"
                     /translation="MTDKLNLTEEQWRERLSPEQYHVLREAGTERAFTGKYESNKAKG
                     MYFCAGCGAPLFDADTKYNSGSGWPSYTSPVAAGAVDEHVDRSHGMTRTEVRCAACEG
                     HLGHVFPDGPGPTGLRYCINSASLDFKPKE"
BASE COUNT          519 a         1067 c         1065 g          555 t
ORIGIN      
        1 ggccatcgcc ggggcagaac cggcaaaaac cagacaaatc gcgagcacag ccatcaggaa
       61 atagccgtac aacttggcaa cgctgctgta tttattcatg ttacgcgaag tcccccagtg
      121 acttcactct aacaagcgat ccggccatct gcaatcgcac cgctgctgag acgcagggaa
      181 agtcaacaga acaagcgcta ggcggcgtgg acaaattcca cgcgtccacg gctggagtca
      241 aaagccgtca attcaaggag gtaaggcgaa ggagtatgtc gatacttcga gccgaaccga
      301 cgcggaagtg gcggctattg gccgggcccc tgcggggttg gcctgtaaaa ggccccgtcc
      361 gcgttgccgc ggccttgaaa gagacccact ctgtctgcgc ccgcgcgcct tgccagggcc
      421 ttttacaggc caaccctggg cgcgcggaat ttgtccacgc cgcctaatac ccctgcccgc
      481 cactaccagc gtcgcgcggc ggcacctgcc gcccggtcgc gcggtcgagg aacccgtcgt
      541 cggcggcagg aggctggccg tctccggcgg ggggctggcc gttgtcttcc gggcggccgg
      601 gggactggtt gaccgccgcg ccctgctgtc ggttgggatc gatcaggttg ccgttctcgt
      661 cgacgtagta atagttttcg gggttgccgc ccatgttggc ttcatcgtcg ggctccaacg
      721 tatattcggg cagcttaagt gccgtgtcga actgctcgac cgggcgccgg gcggtcgcca
      781 cccgcatgta ctgggcaaag gcgcgcgccg gggccgtgcc gcccatcagg ccggggatcg
      841 gcttggcgtc gtcgcgcccc atccatacgc cggtggtgat cccgctggag aagccgatga
      901 accagccgtc cttgttcgag ctcgtcgtcc cggtcttgcc cgccaccggc cgtccgatct
      961 gcgcggcgcg gccggtgccg atgttgaccg cggtctgcag caggtcggtg atcccggcgg
     1021 cgacatagtc ggccaccagc tgctgccccg gcgctggctt gtgctcatag agcacctgcc
     1081 cgtcggtcgt cgtcaccttg gagatgccat agggctcgac ggccatgccc cgcgccgaaa
     1141 ccgcggcgaa ggcccgggtc atgtcgatca ggcgcacatc ggaagtgccc agcaccatgg
     1201 agggcatcgt gttgatcggc gtgctgatcc cgaagcgccg agccatgccg gccaccgtgc
     1261 cgaagcccac ttcgttgccg agctgcgcgg cgacggtgtt cttggagtag gcaaaggccg
     1321 tccgcacgtc gatcttgccg gcgaagttgc gggcatcgtt gtggggcgac cacccctgga
     1381 tcgtcaccgg ttcatccacc accgtatcgt cgggcttgta gccggcttcc agcgcggcga
     1441 gatagacgaa cagcttccac gccgagcccg gctggcgcac cgcgttggtg gcccggttat
     1501 agttggacgt cacatagtcc gtgccgccca ccatggcgag gatcgcgccg tcgcggtcca
     1561 tcgagaccag cgcgccctgc gccccgcccg gcgcgttctt ggcaatcgcg tcggtcgcca
     1621 cccgctgcgc gcccgggtcg agcgtggtcc acacctcgat cggctggttg ttgtcgggca
     1681 gcagcaggtc aagctgcggc agcgcccagt cggtgaagta gcggaccgag ttctgccccg
     1741 cctcctgcgc gaaggtcacc ttggtgaaat cggccgcggc agcttcggcg ggcgtaatcg
     1801 cgccggcatc ctgcatggtc tccaccacga ccttggcgcg gctgacggca gccgccttgt
     1861 cggcggtagg cgaatagcgc gagggcgcct tgacgagccc ggcgatgatc gcggcctcgg
     1921 gcagggtcag ctgatcgccg ggatggccga agaacttgcg gctggcgctg tcgattccat
     1981 aggccccccc gccgaagtag accttgttca ggtaaagctc gaggatctgc tgcttggaga
     2041 acttggtctc cagcgcgatc gacaggatgg cctcgcggat cttgcgggta aaggtgcggt
     2101 tgttgttgag gaagacgttg cgggccagct gctgggtgat ggtcgagccg ccctgccgcc
     2161 atttgccgct ctccacccgc acgaacagcg agcgcaggat gccaacgggg tccaccccga
     2221 agtgatcgta gtagcgccga tcctcgaccg agatcatcgc gtccttcatc gtcgcgggga
     2281 tctgggaaat ggagatccac ttgccgtagc tcgggcccat cgtcaccagc tccgacccgt
     2341 cgcgcgcccg cacgacgatc atctggccct gggcttgcga cttcagcgcc tggtagccgg
     2401 gcagcgacga ggccgcgacc atcaccgccg cagccagggc gaggaccccg gcgaggcaga
     2461 gagccaagcc ccagatcagc agcttgcgga agaagcgccc gatccgcccc ggaccacgcc
     2521 tgcccccacc cgaaccgccg gactgcttgc gcggcggcgg gggtggcgga gccgaaggct
     2581 gttcggacag gctgcgccgg atggcggaac gggccatgta tctagtctga tccctccgcg
     2641 ggcgccggat tggcccgcaa cacttgcggc ccttgcctag ccgaaccgga acgcagcgtt
     2701 aacaacgaaa ttccgctggg gacgcgagtt cattccttcg gcttgaaatc gaggctggcg
     2761 ctgttgatgc aataacgcag ccctgtcggc ccgggaccgt cgggaaatac gtgtccgagg
     2821 tgtccctcgc aggcggcgca gcgcacttcg gtgcgggtca tcccgtgcga ccgatcaacg
     2881 tgctcatcca ccgccccggc ggccaccggg ctggtgtagc tcggccagcc ggaaccggaa
     2941 ttgtacttgg tatcggcatc gaacagcggc gcgccgcagc cggcgcagaa gtacatgccc
     3001 ttggccttgt tgctttcgta cttgccggtg aaggcccgct cggtcccggc ctcgcgcagc
     3061 acgtgatact gctcggggct gagccgctcg cgccattgtt cttcggtgag gttcagtttg
     3121 tcggtcatcg gcaagtctcc tttgcgcgcc catatgggga ggcgcaccgc ttggcgccag
     3181 cctacagggc cgcgaacagc gccagc
//
LOCUS       DATNBV010000452         2478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2174931,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000452 DATNBV010000000
VERSION     DATNBV010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174931"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VN627_10715"
     CDS             <1..551
                     /locus_tag="VN627_10715"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXD03766.1"
                     /translation="VLWLHREASRWLAGVVTVFLAVAIAIPSLALWLRRRGSSVLPAW
                     LDGVGPIRRVLDFISAAPAGLVRRPRLIAQVTLFNGLIFLLDAGTLSVCLLSIGVGAH
                     PAGAFCALVMASIATTLTPVPMGLGTFEATGVAMLRLTGVPFEAAVTAILLLRGLTLW
                     LPLLPGLILLRRTRKEESDGQG"
     gene            538..>2478
                     /locus_tag="VN627_10720"
     CDS             538..>2478
                     /locus_tag="VN627_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HXD03767.1"
                     /translation="MGKARRASRSLPATVGFWRTVDLPAADTAGLSEAEAAARLASDG
                     PNALEETTSRGLPRRILRRVAEPLVLILLASAIVSGLAGDTASLIIILVIVTLSVGLD
                     VYQEHGAEAAAAALRRSIAPTVRVIREGREQVIPSAELVRGDIVRLAPGNLVPGDGVI
                     VVSRSLRVDEAILTGEPFPVEKRRGPSNAAEMAEAFDALFAGTAVVSGEATMLVVATG
                     RATAFGAISAEIVAAQPPSAFERGLHRLGLLIVRLTLALVLFVLLVQLVLHRPPLESF
                     MFAVALAVGLTPELLPMVTTVTLSRGALRMARHKVIVKRLAAIHDLGAMDVLCTDKTG
                     TLTEARITLSAAIGPDGAADDRVLALAAINSALGGSIGNPLDEAILAEHADGTGGWTH
                     VADLPFDFERRRASVLAARGTERLLIVKGAAEAVLPLCTTVATGAGEAPLDDRRRQAV
                     EQQLTERAGKGERVLAVAWRRVPEGGARLGPDDERQLTLAGLCAFSDPPKPSAAAAIA
                     RLGDLGVRVKVISGDAPSVVSHLVVTIGLTAGTVLTGEEIARLSERALRRQVGRTDYF
                     ARVNPDQKLRIIRALSEAGHTVGFMGDGINDAPAIRAADAGLSVDTATDVARGAADLI
                     LLDKDLGVIADGVEEGRRTYANI"
BASE COUNT          341 a          849 c          862 g          426 t
ORIGIN      
        1 ttgtgctgtg gctccatcgc gaggcttctc gctggctcgc cggggtcgtc accgtcttcc
       61 tcgccgtggc catcgccatc ccctcgctgg ccctgtggct gcgccggcgg ggcagttcgg
      121 tcctgccggc gtggctggac ggggtcggcc cgattcgccg cgtgctcgac ttcatcagcg
      181 ccgcgccggc aggcctcgtg cgccggccgc gcctgatcgc acaggtgact ctattcaacg
      241 ggctgatctt tcttctcgac gccgggacat tgtcggtatg cctgctgtca atcggcgtcg
      301 gcgcccatcc ggcgggcgcc ttctgcgcac tggtcatggc ctcgatcgcg accacactca
      361 cgccggtgcc aatggggctg ggcacgttcg aggcgaccgg cgtggcgatg ctcaggctca
      421 ccggagtgcc attcgaggcc gcggtcacgg caatcctgct actgcgcgga ctgaccctgt
      481 ggctgccgct gctgcctgga ctgatcctgc ttcggcgcac acgcaaggag gaatcggatg
      541 ggcaaggcta ggcgtgcatc gcggtcgctc cccgcgaccg tcggtttctg gcgcacggtc
      601 gatcttcccg ctgccgacac cgccgggttg tcggaggccg aagctgccgc ccggctggcc
      661 tccgatggtc ccaacgcact cgaggagacc accagccgtg gactgcctcg ccgcatcttg
      721 cggcgggtcg cagagccgct ggtgttgatc ctgctcgctt ccgcgatcgt ttcagggctc
      781 gccggcgata ccgccagcct gatcattatc ctcgtgatcg tcacgttgtc ggtcggtctc
      841 gacgtctatc aggagcatgg cgccgaagcc gcggcagccg ctctgcgccg ttcgatcgct
      901 cctactgtcc gggtaatccg ggaggggcgc gaacaggtta ttccaagcgc cgagcttgtt
      961 cgcggcgata tcgtccgcct cgcgcccggc aacctcgtgc cgggggacgg ggtgatcgtc
     1021 gtcagccgct cgctccgggt cgatgaggcg atcctgaccg gcgaaccatt cccggtggag
     1081 aaacgccgcg gcccaagcaa tgccgcggag atggcggaag ccttcgacgc gctgttcgca
     1141 ggaacggcgg ttgtctcggg cgaggcgaca atgctggtcg tcgcgaccgg ccgtgcaacg
     1201 gccttcggtg cgatctctgc ggagatcgtg gccgcccagc cgccgtcggc gttcgagcgc
     1261 gggctccacc ggctcggcct gctgatcgtc cggctgacgc tggccctggt cctgtttgtg
     1321 ctgctggtgc aactcgtgct gcatcgcccg ccgctggaaa gcttcatgtt cgcggtggct
     1381 ctcgcggtgg gcctgacccc ggaactgctt ccaatggtga caacggtcac gctgtcgcgc
     1441 ggggcactgc gcatggccag gcacaaggtg atcgtgaaac gcctggccgc gatccacgat
     1501 ctcggcgcca tggacgtgct gtgcaccgac aagaccggca cgctaactga ggcacgcatc
     1561 acgctgagcg cagccatcgg ccccgacggc gcggcggacg atcgggttct cgcccttgcc
     1621 gccatcaaca gcgcgctcgg cggcagtatc ggcaatccgc tcgacgaggc gatcctggcc
     1681 gagcacgccg acggcaccgg cggctggacc catgtggcgg acctgccgtt cgacttcgaa
     1741 cggcgccgcg cgagcgttct cgccgccagg ggcacggagc gcctgctcat cgtcaagggc
     1801 gccgccgaag ccgtgctccc gctttgcacc accgttgcga ccggggcagg agaggcaccg
     1861 ctcgacgacc ggcgcaggca ggcagtcgag cagcagctga ctgagcgcgc cggcaaaggg
     1921 gagcgggtgc tcgccgtggc ttggcggcgc gtgcccgaag gtggcgcacg gctcggcccc
     1981 gacgacgagc ggcagctgac ccttgctggc ctgtgtgcgt tcagcgatcc gcccaagcct
     2041 tcggcagcgg cagccatcgc ccggctgggc gacctcggtg tgcgggtaaa ggtgatctcc
     2101 ggcgatgccc cctcggtcgt gagtcacctc gtggttacga tcgggctcac cgccggcacg
     2161 gtcctgacgg gcgaggaaat tgcccggctg agtgagcggg cactgcgccg gcaggtgggg
     2221 cggacggact atttcgctcg cgtgaaccct gaccagaagc tgcgcattat ccgcgccctt
     2281 tcggaggccg ggcatacggt cggtttcatg ggcgacggga tcaatgatgc cccggccatc
     2341 cgcgccgccg acgcgggcct ctcggtcgac accgccaccg atgtggcgcg gggcgcggca
     2401 gacctgatcc ttctcgacaa ggacctgggg gtgatcgccg atggggtgga ggagggacgc
     2461 cggacctacg ccaacatc
//
LOCUS       DATNBV010000453         2642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1238661,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000453 DATNBV010000000
VERSION     DATNBV010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238661"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VN627_10725"
     CDS             complement(<1..390)
                     /locus_tag="VN627_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03768.1"
                     /translation="MPAGSRRQSGRSAIADQAELAGTLTPLARLALAYAPAPTREDWL
                     TWLGLDARLASVVRSAREPILAQLKLAWWRDRLTGDSAAWPKGEPLLARLAGWDDRAG
                     LTALVDGWEALLDEPPLGRATLETFAEG"
     gene            complement(344..1726)
                     /locus_tag="VN627_10730"
     CDS             complement(344..1726)
                     /locus_tag="VN627_10730"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HXD03769.1"
                     /translation="MAVDLKMPALSPTMEEGTLARWLVNEGDAIKSGDIIAEIETDKA
                     TMEFEAVDEGTLEKILVPAGTAGVKVGTTIATIAGEDAAPASPAKTEAPAQAQKAEES
                     EAPPQAKAPSGTEQLAATAAPKTDPAVPAGTNMKSVTVREALRDAMAEEMRRDGRVFV
                     MGEEVAQYQGAYKVTQGLLDEFGPKRVIDTPITEYGFAGIGTGAAMGGLRPIVEFMTF
                     NFAMQAIDHIINSAAKTNYMSGGQMRCPIVFRGPNGAAARVGAQHSQNYAPWFSAVPG
                     LVVIAPYDASDAKGLLKAAIRSEDPVVFLENELVYGRSFELPELDDHVLPIGKARVVR
                     EGRDVTIVSYSIGVGLSLEAAETLAGEGIEAEVLDLRTLRPLDREAVLASLRKTNRMV
                     VAEEGFPVCSIASEIIAICMEEGFDDLDAPVLRVCDEDVPLPYAANLEKAALIDAGRI
                     AAAVRKVCYR"
     gene            complement(1726..>2642)
                     /gene="pdhA"
                     /locus_tag="VN627_10735"
     CDS             complement(1726..>2642)
                     /gene="pdhA"
                     /locus_tag="VN627_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HXD03770.1"
                     /translation="RFEERAGQLYGLGLIGGFCHLYIGQEAVAVGLQSALSEGHDSVI
                     TGYRDHGHMLAYGIDPKVIMAELTGRAAGISHGKGGSMHMFSTEHKFYGGHGIVGAQV
                     PLGAGLAFAHKYREDGGLCMAYFGDGAANQGQVYETFNMAALWNLPIIFVVENNGYAM
                     GTAVTRGSAETHFHRRGTAFRIPGMNVNGMDVLEVRAAANIAVDYVRAGNGPVLMELH
                     TYRYRGHSMSDPAKYRTREEVQEMREKHDPIESAKMELIARGVAEDKLKEIDKRIRAQ
                     VSEAADFAENSPEPASPELYTDVLVERY"
BASE COUNT          421 a          912 c          839 g          470 t
ORIGIN      
        1 gccttcggca aatgtctcga gtgtcgcccg gccaagcggc ggctcgtcaa gcagggcttc
       61 ccagccatcg accagcgccg tgagcccggc ccgatcgtcc catcccgcca gccgggccag
      121 caatggctcg cccttgggcc atgcggcgga atcgcccgtc agccggtcac gccaccaagc
      181 cagcttgagc tgggccagga tcggctcacg cgcggaacgc acgacgctag ccagccgcgc
      241 atcgagcccc agccaggtca gccagtcctc gcgcgtcggc gccggagcat aagccagggc
      301 cagccgtgcc agcggcgtca gcgtgcccgc aagttcggcc tgatcagcga tagcagacct
      361 tccggactgc cgccgcgatc cggccggcat cgatcagggc agccttttcg agattggccg
      421 catagggcag cggcacgtct tcgtcgcaga cccgcagcac cggggcgtca agatcgtcga
      481 agccctcctc catgcagatc gcgatgattt cggaagcgat cgagcaaacc gggaagcctt
      541 cctcggcaac aaccatgcgg ttggtcttcc tgaggctggc gagcacggct tcccggtcga
      601 gcggacgcag ggtgcgcaga tcgagcactt cggcctcgat cccctcgccc gccagcgtct
      661 cggctgcctc cagtgatagt cccacgccga tcgagtagga cacgatggtc acatcgcgtc
      721 cctcgcgcac cacccgcgcc ttgccgatgg gcagcacgtg atcgtccagt tccggcaatt
      781 cgaacgagcg gccatagacg agctcgttct caaggaagac caccgggtcc tcgctgcgga
      841 tggccgcctt gagcaggccc ttggcgtccg acgcatcata gggcgcgatc accaccagcc
      901 ccggcacggc ggagaaccat ggcgcgtagt tctggctgtg ctgcgcgccc acgcgcgccg
      961 ctgcgccatt gggcccacgg aacacgatcg ggcagcgcat ctgccctccc gacatgtagt
     1021 tggtcttcgc ggccgagttg atgatgtgat caatcgcctg catcgcgaag ttgaaggtca
     1081 tgaactcaac gattggccgc agcccgccca tggcggcacc ggtgccgatg ccggcgaagc
     1141 cgtattccgt gatgggcgta tcgatcacgc gcttggggcc aaactcatcg agcaaccctt
     1201 gcgtgacctt gtaggcgccc tggtactgcg ccacttcctc gcccatcacg aagacccggc
     1261 catcgcgccg catttcctcg gccatggcat cgcgcaaggc ctcgcgcacc gtgaccgact
     1321 tcatgttggt gccggccggg acggccggat cggtcttggg cgcggcagtt gcggccagct
     1381 gctcggtgcc gctgggcgcc ttggcctgcg gcggggcttc cgattcctcc gccttctggg
     1441 cctgggccgg cgcttcggtc ttggcgggcg aggccggagc ggcatcctcg ccggcaatcg
     1501 tggcgatggt cgtgccgacc ttcaccccgg ccgtgccggc ggggaccagg atcttctcca
     1561 gcgtgccttc gtcgacggct tcgaattcca tcgttgcctt gtcggtttcg atctcggcga
     1621 tgatgtcgcc agacttgatc gcatcgcctt cgttgaccag ccagcgggcg agcgtgcctt
     1681 cctccatcgt gggcgaaagc gcgggcatct tgaggtcgac agccatcagt agcgctccac
     1741 caggacgtcg gtatagagtt cgggagacgc cggttcgggc gaattctcgg caaagtcagc
     1801 cgcttcggac acctgggcgc ggatgcgctt gtcaatctcc ttgagcttgt cttccgccac
     1861 gccccgcgcg atcagctcca tcttggcgct ctcgatcgga tcgtgcttct cgcgcatctc
     1921 ctgcacttcc tcgcgcgtgc gatacttggc cggatccgac atcgaatggc cgcgatagcg
     1981 gtaggtgtgc agttccatca gcaccgggcc attgcctgcg cgaacatagt ccaccgcgat
     2041 attggccgcg gcacgcacct cgagcacgtc catgccgttg acgttcatgc cgggtatgcg
     2101 gaaggccgtt ccgcggcggt ggaagtgggt ctcggccgag ccgcgggtga cggccgtgcc
     2161 catggcatag ccgttgttct cgaccacgaa gatgatcggc aggttccaca gggccgccat
     2221 gttgaatgtc tcgtagacct ggccctggtt cgccgcgccg tcgccgaagt aggccatgca
     2281 cagcccgccg tcctcgcggt acttgtgggc aaaggcgaga cccgcgccga gcggcacctg
     2341 ggcgccgacg atgccatggc cgccgtagaa cttgtgctcg gtgctgaaca tgtgcatcga
     2401 gccgcccttg ccgtggctga tgcccgcggc ccgcccggtc agctcagcca tgatcacctt
     2461 gggatcgatc ccgtaggcca gcatgtggcc atggtcgcga tagccggtaa tgacgctgtc
     2521 gtgcccttcg ctgagcgccg actgaaggcc gaccgcgacg gcttcctggc cgatatagag
     2581 atgacagaaa ccgccgatca ggccgagacc atagagctgc cccgcccgtt cctcgaaccg
     2641 gc
//
LOCUS       DATNBV010000454         3546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_269553,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000454 DATNBV010000000
VERSION     DATNBV010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269553"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VN627_10740"
     CDS             <1..431
                     /locus_tag="VN627_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03771.1"
                     /translation="LGPILCARAGIPLMGRGSQLIHVTSESVETRHPMLSIYQAGKAG
                     LERFSRSMVEELAPRGIRSIIFRAGQMQGEGMSTNFDPAILPRFAEMNAARGIDPTAR
                     GFSGYASAAQVMRNVVDLPLDISVEIVSCHSLPGEFPTEV"
     gene            561..857
                     /locus_tag="VN627_10745"
     CDS             561..857
                     /locus_tag="VN627_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03772.1"
                     /translation="MQLKAGTKLHSAVCDTDVMVIKGSGDVNLTCGGAPMTAARGDLG
                     ELDAALAGGTAMGKRYTNADQSVELLCIKPGKGTLAIDGVALELKSAKPLPSSD"
     gene            891..2390
                     /locus_tag="VN627_10750"
     CDS             891..2390
                     /locus_tag="VN627_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXD03773.1"
                     /translation="MNISLVLQMASDTFPDRIALTHNGVHYSYGRLFASANAAAARFA
                     ESGADYVSYLGESGPGMVIALFGAAMAGKPFAPLNYRLTGPETLRLVERLYPVYLIAD
                     EDKIAGIPADAARVLISIPEFLAAVTTDEAEPFPAPDEDDEAIAVQLFTSGTTGEPKA
                     AVLRNQNMLGYVLGSVEFASAEEDEATLSTVPPYHIAGISAILSGIYAMRRIVQLPNF
                     APDAWLQLCRDQSVTNAFVVPTMLSRIIAHIDETGTPADLPAMRALAFGGGKMPLPVV
                     QRALDLWPNVNFANAYGLTETSSTIAILGPEDHREAHNHPDPMVARRLDSVGKPLPTV
                     EIEIRGEDGTVLGPNQLGEVFVRGPQVSGEYQGRKLTDEDGWFPTRDSGFIDDAGFLF
                     LAGRIDDVIVRGGENMSPGEIEHVLREHDAVEDVAVLGVASVGWGEAPVAAVVPKPGA
                     SATEAELKELVRQKLRSSRVPERIVFVEALPYNEMGKLLRRKVKDLFDA"
     gene            2407..>3546
                     /locus_tag="VN627_10755"
     CDS             2407..>3546
                     /locus_tag="VN627_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXD03774.1"
                     /translation="MTAWADAALAEIRSLALSPARLTMDAATLLAERAALMKLPRPGR
                     VSAGGACHLLDCADGAIALSLPRPDDWQLLPALFEDASPCDWQAVEQAVRSRKVDPLV
                     DRARLLGLAVARADVLPLSPTAALAPQRLAPPRQPKPGRRPVVLDLTTLWAGPLAGHL
                     LERGGARVIKVESPTRTDGARRGHAGFYGLLNQDKLCITVDLATAAGRERLLALIDRA
                     DVVLESSRPRALRQLGIDAQALVAHRPGLTWLSITAHGRGAPQEDWTGFGDDAAAAGG
                     LCAAVREATGSPAFIGDALADPLTGIAAVRAAWQAWRSGGGVLASVALSASVAAALHH
                     DRTMFAGEAGSMLARVARDGYPAPPVRPITGPVGAPGAHNDWPLEK"
BASE COUNT          544 a         1226 c         1192 g          584 t
ORIGIN      
        1 tgctcggccc gatcctgtgc gcccgcgccg gcatcccgct gatgggccgg ggtagccagc
       61 tgattcacgt cacttcggaa tcggtggaaa cgcgccaccc gatgctatcg atctaccagg
      121 ccggcaaagc cgggctcgaa cggttctcgc gctcgatggt ggaagagctg gcgccgcggg
      181 gcatccgcag catcatcttc cgcgccggcc agatgcaggg cgaaggcatg tcgacgaact
      241 tcgacccggc gatcctgccc cgcttcgccg aaatgaacgc cgcccgcggg atcgatccga
      301 cggcacgcgg cttctctggc tatgcctcgg ccgcgcaggt gatgcgcaat gtcgtggatc
      361 tgccgctgga catcagcgtc gaaattgttt cctgtcacag tctgccgggc gagtttccga
      421 cggaagtcta gctgtggtag ccaagccgga aaaccgcctg tgtgctgcgg ctgagtgcgg
      481 ccttgtgcgc ttagggaacg acgataaacc tcaacgaatg gggcggctgc gggaagtcaa
      541 ttatggagca gaatctggat atgcaactga aggcaggcac caagctgcac agcgcagttt
      601 gcgataccga cgtgatggtc atcaagggca gcggtgacgt caatctcacc tgcggcggcg
      661 ctccgatgac cgcggcccgc ggcgatctgg gtgagcttga tgccgccctt gccggcggca
      721 ccgccatggg caagcgttac accaatgccg accagtcggt cgaactgctc tgcatcaagc
      781 cgggcaaggg tacgctggcg atcgacggcg tggcgctcga actgaagtcg gccaagccgc
      841 tcccgtcgtc ggactgagct tcggcccctg gctggcggat cggcgacgcg atgaacattt
      901 ccctcgtcct gcagatggcc tccgatacct tcccggatcg gatcgccctc acgcataatg
      961 gcgtccacta cagctatggc cggctgttcg cctcggccaa tgcggccgcg gcgcggtttg
     1021 ccgaaagcgg ggcggactat gtctcgtacc tgggcgaaag cgggccgggg atggtcatcg
     1081 cgctgttcgg cgcggccatg gcgggcaagc ccttcgcgcc gctcaactat cggctgaccg
     1141 ggccggaaac cctgcgcttg gtcgagcggc tctacccggt ctacctgatt gccgacgaag
     1201 acaagatcgc cggcattccc gccgatgcgg cccgggtcct gatcagcatt cccgaattcc
     1261 tcgccgccgt gacgacggac gaggccgagc ctttccccgc accggacgag gatgacgagg
     1321 cgatcgcggt gcagctgttc accagcggca ccaccgggga gcccaaggcc gcggtcctgc
     1381 gcaaccagaa catgctcggc tatgtgctcg gctcggtcga attcgcctcg gccgaggaag
     1441 acgaagctac gctgtcaacg gttccgccct atcacatcgc cggcatttcc gcgatcctgt
     1501 ccggcatcta tgcgatgcgc cggatcgtcc agctgcccaa cttcgccccc gacgcctggc
     1561 tccagttgtg ccgcgatcaa tctgtcacca atgccttcgt cgtgccgacc atgctcagcc
     1621 ggatcatcgc ccatatcgat gagactggca ccccggccga tctcccggcg atgcgcgccc
     1681 tggctttcgg cggcggcaag atgccgctcc cggtcgtgca gcgcgcgctc gatctgtggc
     1741 cgaacgtcaa cttcgccaat gcctacgggc tgacggagac cagctcgacc atcgcgatcc
     1801 tcggccccga ggatcaccgc gaggcgcaca accatcccga tccgatggtc gcccgccggc
     1861 tggattcggt tggcaagccg ctaccgacgg tcgagatcga gatccgcggc gaagacggca
     1921 ccgtgctcgg gcccaaccag ctgggcgaag tcttcgtacg aggcccgcag gtctcgggcg
     1981 aataccaggg ccgcaagctg accgatgaag acggctggtt tccgacccgc gattccggct
     2041 tcatcgacga tgccggcttc ctgttcctcg ccggccggat cgacgacgtg atcgtgcgcg
     2101 gcggcgagaa catgtccccg ggcgagatcg agcatgtcct gcgcgagcat gacgcagttg
     2161 aggatgtcgc cgtgctcggc gtggcctcgg tcggatgggg cgaggccccg gtcgccgcgg
     2221 tcgtgccgaa acctggcgcc agcgccaccg aggccgagct caaggaactg gtgcgccaga
     2281 agctgcgctc ttcgcgcgtg ccggagcgga tcgtcttcgt cgaggcgctg ccctacaacg
     2341 agatgggcaa gctgctgcgg cgcaaggtca aggacctgtt cgacgcctga tccgctgccc
     2401 ctgccgttga ccgcatgggc ggacgcggcg ctggccgaga tccgcagcct ggccctttcc
     2461 ccggcgcggc tcacgatgga tgcggccacc ttgctggccg aacgggccgc gctgatgaag
     2521 ctgccccgcc cgggccgggt ctccgccggc ggcgcctgcc acctgcttga ctgcgccgat
     2581 ggcgccatcg ccctcagcct gccccgaccg gacgactggc aattgctccc cgccctgttc
     2641 gaggatgcgt cgccctgcga ttggcaggcg gtcgaacagg cggtgcgctc ccgcaaggtt
     2701 gacccgctgg tagaccgggc ccggctgctc ggcctcgctg ttgcccgcgc cgatgtcctg
     2761 cctttgtcgc cgacagcggc tcttgcgccc cagcgcctgg ctccgccgcg ccagccgaaa
     2821 ccgggccgcc ggccggtggt tctcgatctg accaccctgt gggccggccc gctcgcaggg
     2881 cacctgctcg aacgcggcgg ggcgcgggtg atcaaggtgg agagccccac ccgcaccgat
     2941 ggcgcccggc gcggccatgc cgggttctac ggcctgctca accaggacaa gctgtgcatt
     3001 actgtggacc tcgccacggc cgccgggcgc gagcgcctgc tcgcgctgat cgaccgggcc
     3061 gacgtggtgc tggagtcgag ccggccgcga gccctgcgcc agcttggcat cgacgcccaa
     3121 gcgctggtcg cccacaggcc gggcctcacc tggctgtcga tcaccgccca tggccggggc
     3181 gcaccgcagg aggactggac cgggttcggc gacgatgccg ccgcggctgg ggggctgtgc
     3241 gcggcggtac gcgaggcgac gggcagccct gccttcatcg gcgatgccct ggccgatccg
     3301 ctgaccggga ttgccgccgt acgggcagcg tggcaggctt ggcgcagcgg cggcggggtg
     3361 ctggcatcgg tcgctctgtc tgccagtgtt gcagcggcgc tgcaccacga tcgcaccatg
     3421 tttgcaggcg aggcggggag catgctcgcc agggttgccc gtgacggata tcctgcgccg
     3481 cctgttcgtc caatcaccgg cccggtcggt gccccgggcg cgcataatga ctggccgctg
     3541 gaaaag
//
LOCUS       DATNBV010000455         3232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3754639,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000455 DATNBV010000000
VERSION     DATNBV010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3754639"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VN627_10760"
     CDS             complement(<1..300)
                     /locus_tag="VN627_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HXD03775.1"
                     /translation="MEHTVLPSWALLAYLASGVLFILALRGLSSPATSRAGNRYGMVG
                     MGIAIVTTLATQAPVPPGDWWPRPTPADLVILAKILAAIALGAMIGLTTARKIQMT"
     gene            complement(308..604)
                     /locus_tag="VN627_10765"
     CDS             complement(308..604)
                     /locus_tag="VN627_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HXD03776.1"
                     /translation="MDFISILSIFVLACFVGYYVVWSVTPALHTPLMAVTNAISSVII
                     VGALVAAAASGGGVRALTSSWLGLAAVTLASINIFGGFAVTARMLAMYKSKKKG"
     gene            complement(796..942)
                     /locus_tag="VN627_10770"
     CDS             complement(796..942)
                     /locus_tag="VN627_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03777.1"
                     /translation="MRTAILRERKARETRVAATAETVRKVFARAQTMDLRSSQPDIAA
                     GLLE"
     gene            complement(963..1088)
                     /locus_tag="VN627_10775"
     CDS             complement(963..1088)
                     /locus_tag="VN627_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HXD03778.1"
                     /translation="MSSSNDIKSSQQTYTGFISMAKWGTIGAMLVAALVVFLITR"
     gene            1293..2693
                     /locus_tag="VN627_10780"
     CDS             1293..2693
                     /locus_tag="VN627_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXD03779.1"
                     /translation="MLIDDEPAQSRLVSALAAREGWRTLVVRDSETAIATLGTRQGMQ
                     LSAIILDQWVPGEEACDLIAELKARRPALPILMLTASASPLLAVEAMRAGATDYLIKP
                     VAPERLMHALRAATTREAPRDELAPLTEKMPGPPSFDAMIGAAPTFRAALAVAAKAAR
                     GHNHVLIEGETGTGKEMVVRAMHAASPRAKAPLRIINVGATPANSIESLLFGHEKGAF
                     PGAFDRHVGALQNCDGGTLVIDEIDRLPPSVQDRLLESLQRGDVRPIGARHSFRIDLR
                     LIAASNYPLADLVAGGHFLPGLHDLLAPVSVMLPPLRERSQDISALARHFLARIGELP
                     GLRRLGITDGALSLLAAYDWPGNVRQLQATLFRACVFCEGDALTAADFPQLSNMLGVD
                     QQVTPGNQAVHGSPGVLLYTSDGNLRALEDIEADVIRLAIGHYRGRMTEVARRLGIGR
                     STLYRKLSELGIDSAA"
     gene            2698..2949
                     /locus_tag="VN627_10785"
     CDS             2698..2949
                     /locus_tag="VN627_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03780.1"
                     /translation="MRRLPTRAAAALAFLILMFSCLALGMGLTTPQVRGGLFWLVSAG
                     FAIQALASAAWLVRLIAERLGAERMANATDRGTRQLSGD"
     gene            2996..3205
                     /locus_tag="VN627_10790"
     CDS             2996..3205
                     /locus_tag="VN627_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03781.1"
                     /translation="MSLHSIIEPAGTLMTGWKLVPKRDCHAADVRNEDDIPLEIACTP
                     APAENAEEIKPASKWESDRKLSDVG"
BASE COUNT          529 a         1080 c         1094 g          529 t
ORIGIN      
        1 cgtcatctgg atcttgcggg ccgtggtgag gccgatcatc gcgcccagcg cgatggcggc
       61 gaggatcttg gcgaggatga cgagatcggc cggcgtgggg cgcggccacc agtcgcccgg
      121 cgggaccggc gcctgggtgg ccagcgtggt gacgatggca atgcccatgc cgaccatgcc
      181 atagcggttg cccgcgcggc tggtggcggg cgaggagagg ccgcgcaggg cgagaatgaa
      241 gagtacgcct gaggcgaggt aggccagcag cgcccaggac ggcaggaccg tgtgttccat
      301 gcgccgttca gcccttcttc ttcgacttgt acatggccag catccgcgct gttaccgcga
      361 agccgccgaa gatgttgatc gaggccagtg tcaccgccgc cagccccagc cacgaggagg
      421 tcagcgcccg cacgccccca cccgaagccg ccgcggcgac cagcgcgccg acgatgatga
      481 cggaggagat ggcgttggtc acggccatga gcggcgtgtg caaagcgggg gttaccgacc
      541 agacgacgta gtagccgacg aagcaagcca gtacgaagat cgagaggatg gagatgaagt
      601 ccatgtcagc gagatgctcc gatagaggct tcccccatgg gggagatagc agcccaccgg
      661 gctgacggag gagcgctgaa cgagccaagc cgcgatagtg gctgcgcgct gccgcgcgcg
      721 aattgcttgc cccctaccac cgctttgcgg ccctcctccc ccggtggggg aggttgttgg
      781 cagggcaatc tggattcact ccagcagccc tgccgcgata tcgggctgac tggaccgaag
      841 gtccatcgtc tgcgcacggg cgaagacttt gcgcacagtt tccgcggtcg cggcgacgcg
      901 ggtttcccgc gccttgcgtt cccgcaggat tgctgtccgc atgccgcccc gcagcgcgag
      961 gatcagcggg tgatcaggaa aacgacgagc gcggcaacca gcatggcgcc aatggtgccc
     1021 cacttggcca tgctgatgaa gccggtgtag gtctgctgcg aggacttgat gtcgttggac
     1081 gaggacattt gcgccgttac ccttcgaatt gacgcccgtg aattcgcagc gcctgcttac
     1141 agcccagcct ctatcgcgcc gcgccgcgcc actcaagccc gacgcttgcc cacagatcag
     1201 ccttaagctg ctttttaccg ttcgctgata atccgaccgg cgtggaagcc ataacgcgca
     1261 cacctcttgc cgacagcgag caacgcctgc tcatgctgat cgatgacgag ccggcccaga
     1321 gccggctggt ttcggccctc gccgcccgcg aaggttggcg cacgctggtc gtgcgcgatt
     1381 cggaaaccgc gattgccacg ctcggcacgc gccagggaat gcagctttcg gcgatcatcc
     1441 ttgaccagtg ggtgccgggc gaggaggcct gcgacctgat tgccgaattg aaggcgcggc
     1501 gccctgccct ccccatcctg atgctgacgg ccagtgcctc gccgctactg gcggtggaag
     1561 ccatgcgcgc cggggcaacc gactacctga tcaagccggt ggcgccggaa cggctgatgc
     1621 acgccctgcg cgccgcaacg acacgcgagg cgccgcgcga cgagctggcc ccgctgaccg
     1681 agaaaatgcc cggcccgccg agcttcgatg cgatgatcgg ggcggcgccc acgttccgcg
     1741 ccgcgctcgc cgtggccgcg aaggccgcgc gggggcacaa tcacgtgctg atcgagggcg
     1801 agaccggcac cggcaaggaa atggtggtgc gcgcgatgca cgccgcatcc ccccgtgcca
     1861 aggccccgct gcgcatcatc aacgtggggg ctaccccggc aaattcgata gaatcgctgc
     1921 tgttcggcca tgagaagggc gccttccccg gcgccttcga ccggcatgtc ggcgcgctcc
     1981 agaactgcga cggcggtacg ctggtgatcg acgagatcga tcgcctgccc ccatcggtgc
     2041 aagaccgcct gctggaatcg ctccagcgcg gggacgtgcg accgatcggg gcgcggcatt
     2101 cgttccgcat cgatcttcgg ctgatcgccg ccagcaacta tcccctggcc gaccttgttg
     2161 cgggcgggca ctttctgccc ggcctccacg atctgctcgc gcccgtttcg gtgatgctgc
     2221 cgccgctgcg cgagcgttcg caggacattt ccgcgctggc ccgccatttc ctggcccgca
     2281 ttggcgaact gccgggcctg cggcggcttg gcattaccga cggcgcattg tcgctgctgg
     2341 ccgcctacga ctggccgggc aatgtccgac agctccaggc taccctgttc cgcgcctgcg
     2401 tcttctgcga gggcgatgcc ctgaccgcgg cggatttccc gcagctttcg aacatgctgg
     2461 gggtggacca gcaggtcacc ccgggcaatc aggcggtgca tggctcgccg ggcgtgctgc
     2521 tctatacttc cgacggcaac ctgcgcgcgc tggaggatat cgaggcggac gtgatccgcc
     2581 tggccatcgg ccactatcgc gggcggatga ccgaggtggc ccggcggctg gggatcgggc
     2641 gctccacgct ctaccgcaag ctctccgagc ttggcatcga ttccgcggcc tgattccatg
     2701 cgtcggcttc ccacccgcgc cgctgccgcg ctggctttcc tgatcctgat gttctcgtgc
     2761 cttgcgctcg gcatggggct gaccaccccg caggtgcgcg gcgggctgtt ctggctggtc
     2821 tcggcagggt tcgcgatcca ggcgctggcc tcggcggcct ggctggtgcg gctgattgca
     2881 gaacggctgg gcgcggaacg gatggccaat gcgacggatc gtggaacgcg gcagctgtca
     2941 ggagattaag ccgacataag cggaacttcg tctccgccgg agtcgcacag ttgccatgag
     3001 cctgcacagc atcatcgaac ccgccggcac cttgatgacc ggctggaaac ttgtcccgaa
     3061 gcgcgattgc catgcggcgg atgtgcgcaa cgaggacgac attccgctgg agatcgcctg
     3121 cacccccgcg cccgccgaga acgcggagga aatcaagccc gcctcgaagt gggaatcaga
     3181 ccggaagctg agcgatgtcg gttagggccg catcgcgcag gccgcgccag ac
//
LOCUS       DATNBV010000456         2626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4730782,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000456 DATNBV010000000
VERSION     DATNBV010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4730782"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VN627_10795"
     CDS             <1..410
                     /locus_tag="VN627_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03782.1"
                     /translation="LLVAALWPAAAELDGWPLFVGILLIAGGFIAFIIGMLYKIVPFL
                     AWMHLQSSGQAKVPAPAMNKILGDRDMQRQMLAYVAACVLLLAAVLLPEWLARPAGLA
                     FALAGGWLWWNLLGAARRYRLHLGVIAAKLATP"
     gene            407..787
                     /locus_tag="VN627_10800"
     CDS             407..787
                     /locus_tag="VN627_10800"
                     /inference="COORDINATES: protein motif:HMM:NF016161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HXD03783.1"
                     /translation="MSYLALKHLHITCVVLSGLGFCLRGWWMLRESPLRQHRLARVLP
                     HVVDTLLLGSALTMAWLSGQYPFAQSWLTAKLGGLIAYILLGTMALKRGKTRQARAGY
                     FGLALLAYAYIVSVALTRNPIPFA"
     gene            complement(890..2245)
                     /locus_tag="VN627_10805"
     CDS             complement(890..2245)
                     /locus_tag="VN627_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HXD03784.1"
                     /translation="MSNFRPIDRATPYLLPPSVEEWLPEDHLARFVVEIVDQLDLSAL
                     ERAYRGSGKAAYHPAMLTALLIYGYATGTYSSRAIEKASHDSVAFRYIAANQHPDHDT
                     LCAFRRRFLKEIEALFVQVLQIAGQMKLLKLGTVALDGTKIHANASRHNALSYARAQT
                     IETQLHTEVRELLARAEAADRAPLPAGLSVPEELARREARLAAIAEAKAKIEARATDR
                     HAEEQRQYEAKVAAREAKAKARGKRPGGRPPTPPSGGVRETDQINLTDEESRIMPRSG
                     GGFEQCYNAQAAVDTTSMLIVGARLSNAPVDARQLDPMLDRLALIPEALGRTTDLLAD
                     AGFFSQANVERCRERKVAPLIARRRDQHYVPWLDRLAEPPPFDEAGRPVDQMLHRLQT
                     RAGRALYGLRKQTVEPVFGIIKSVMRFRQFLLRGLQLAGGEWNLVAMAWNIRRMAVLA
                     A"
BASE COUNT          429 a          872 c          807 g          518 t
ORIGIN      
        1 tgctgctggt cgccgcgctg tggccggccg ccgccgaact ggacggctgg ccgctgttcg
       61 tcggcattct gctgatcgct ggcggcttca tcgccttcat catcggcatg ctctacaaga
      121 tcgttccctt cctggcctgg atgcatctcc agagcagcgg gcaggctaaa gtgccggccc
      181 cggccatgaa caagattctg ggcgaccgcg acatgcagcg ccagatgctg gcctacgtcg
      241 ccgcctgcgt gctgttgctc gccgccgtgt tgctgcccga atggctggcc cgcccggccg
      301 ggctggcatt cgcgctggcc ggcggctggc tgtggtggaa cctgctgggc gccgcccggc
      361 gttaccgact gcacctgggc gtgatcgcgg ccaaactcgc cacgccatga gctacctggc
      421 gctgaaacac ctgcacatca cctgcgtggt gctgagcggc ctcggcttct gcctgcgcgg
      481 ctggtggatg ctgcgcgaat cgcccctgcg ccagcaccgc ctggcccggg ttttgccgca
      541 tgtcgtcgat acgctgctgc tcggcagcgc cctgaccatg gcctggctga gcggccagta
      601 tcccttcgcc caaagttggc tgaccgccaa gctcggcggg ctcatcgcct acatcctgct
      661 cggcaccatg gctctcaagc gcggcaaaac ccgccaggcc cgcgccggct atttcgggct
      721 ggccctgctg gcctacgcct acatcgtcag cgtggcgctg acccggaatc cgattccgtt
      781 cgcctagcag cctgtcggat tgacccccgg ccgcgtcgac tttcaggcag caccgttttg
      841 ttggggctga gcgattggat ttcactgagg cggggtggcc ggcgtgcgct caggctgcca
      901 ggacggccat ccggcggata ttccaagcca tcgcgaccag attccattcg ccaccggcca
      961 actgcagtcc gcgcagcaga aactgccgga accgcattac cgacttgatg atgccaaaca
     1021 ccggttcgac ggtctgtttg cgcagcccgt acaaggcccg ccccgcccgg gtttgcagtc
     1081 gatgtagcat ctgatcaacc ggcctgccag cttcatcgaa tggtggtggt tcggccaaac
     1141 gatcgagcca cgggacgtaa tgctgatccc gtcgacgggc aatcagcgga gccaccttcc
     1201 gttcccgaca ccgttcgacg ttcgcctggc tgaagaagcc ggcatcggcc agcaggtcag
     1261 tggtccggcc cagtgcctcc ggaatcagag ccaaccggtc cagcatcggg tccagttggc
     1321 gagcatcaac gggcgcgttg ctgaggcggg ccccgacgat cagcattgag gtcgtatcga
     1381 ccgccgcctg ggcgttatag cattgctcga agcctccgcc ggagcgcggc atgatccggg
     1441 attcttcatc ggtcagattg atctggtccg tctcccgcac gccaccgctt ggtggcgtcg
     1501 gcggccgacc gcccgggcgc ttgccccggg ctttggcctt cgcttcgcgg gccgccacct
     1561 tggcttcgta ctggcgctgc tcctccgcat ggcgatcggt ggcgcgagcc tcgatcttgg
     1621 ctttcgcctc ggcaatcgct gccaacctgg cttcacgccg tgccagttcc tccggaaccg
     1681 agagcccggc cggcaagggt gctctgtccg ctgcttcggc ccgcgccagg agttcccgga
     1741 cttccgtgtg cagttgcgtc tcgatcgtct gcgcccgagc atacgacagg gcgttatgcc
     1801 gcgaggcgtt ggcgtgaatc ttcgtgccgt cgagggcgac cgtcccgagt ttgagcagct
     1861 tcatctgccc ggcaatctgc agcacttgga cgaacagcgc ttcgatttcc ttcaggaagc
     1921 gccgccggaa ggcgcacagg gtgtcgtggt cggggtgctg gttggccgcg atgtagcgga
     1981 aggcaactga atcgtgactg gctttctcga tcgcccgact cgagtacgta ccggtcgcgt
     2041 agccgtagat cagtagcgcg gtcagcatcg ctggatgata ggccgctttg cccgagcccc
     2101 ggtaggcacg ctccagcgcg gacaggtcca gttgatcgac aatctcgacc acaaagcgcg
     2161 ccagatggtc ctccggcaac cactcttcga ccgacggcgg gagcaggtac ggcgtggcac
     2221 gatcgatcgg gcggaaattc gacatggaga ctccctgctt aggtttctcc tatggcaaat
     2281 caattcgccg atcgttcact ctcgaaccat taaatccgac aggctgctag cggcctcgac
     2341 gccagggttg cgcattaggc cagtccagtg aattgtcgaa aactcgacaa gaaaaaaccg
     2401 gcacctggtc ctttgtaatc ctgtttagtt tcattaaaac agatgcttat cgtctgtttc
     2461 cacaggctgg cactgtaact gcaagggggc tccaaccaac gaccggtcaa ggagaacccc
     2521 aatggaacag agaaccgtac cgattaccca ggaagccgcc ctgcgcgtgg ccttggccgc
     2581 ccgtgccatg cccaatgtca ctttgcccag gctgattgcc ttgttg
//
LOCUS       DATNBV010000457         3393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4091659,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000457 DATNBV010000000
VERSION     DATNBV010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4091659"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..862)
                     /gene="rnc"
                     /locus_tag="VN627_10810"
     CDS             complement(188..862)
                     /gene="rnc"
                     /locus_tag="VN627_10810"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HXD03785.1"
                     /translation="MTALADETKAFLQQLTGQAIAADALWVEALTHGSTGEPRNYERL
                     EFLGDRVLGLVIAEWLHERGTGNEGKLSQRLNALVSRDSNAKVARTIGLGQHIRLGKQ
                     ARDDGGADSDNILGDVVEALLGASFLTWGFDRTRDLVRKLWGDAVEGRVGQSKHPKSA
                     LQEWAAGNRRRVPEYRLVERSGPDHAAHFRVSVHVHGVGEVEAEGSSKQEAETAAAAE
                     FLKRFG"
     gene            complement(864..1757)
                     /gene="lepB"
                     /locus_tag="VN627_10815"
     CDS             complement(864..1757)
                     /gene="lepB"
                     /locus_tag="VN627_10815"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXD03786.1"
                     /translation="MTDHTPDPAQSASLDAKPSGQPSTPGKPAKAKKEENFFVFLVKL
                     VLVVLAFRSFIFAPFNIPSESMLPRLENGDYLLASKWSYGFSRYSLPLSVPLIPGRIF
                     ARQPDRGDVVIFKAPPFNDTDYIKRVIGLPGDQIQVTGGVLHINGAAVPKVRVADAVI
                     PEVGNTRCYHPAFERRAADGSPTCQYPRYRETLPGGVSYYVLDFGPYAYRPDPTFDPD
                     NTGVYIVPEGHFFAMGDNRDNSMDSRFPAVPGGGIGIVPQENLVGKAQIMMFSTNGTA
                     SWFKPWTWLTATRWSRIGSTI"
     gene            complement(1984..2142)
                     /locus_tag="VN627_10820"
     CDS             complement(1984..2142)
                     /locus_tag="VN627_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03787.1"
                     /translation="MTLNYRHMLLIAALFMGSLAVMTAIQGYFEHSPVIVIFQFLASA
                     LLLYWAIA"
     gene            2393..>3393
                     /locus_tag="VN627_10825"
     CDS             2393..>3393
                     /locus_tag="VN627_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HXD03788.1"
                     /translation="MTQAAQDPVSAAWSALAALPRPSLADLFAHGDRLGLLATSLDLP
                     GGPIRFDWSKTHLDEASLAGFERLAEATGFAERRRALFAGEPINATEGRAAEHMAQRG
                     LGAEASVEEAASLHARMHMLVEAIHGGAFGEVRHLIHIGIGGSALGPALAIDALARDN
                     PMVDVHVVSNIDGCALEQAFAACDPATTMIAVASKTFTTIETMTNAHSALDWLRENGE
                     ADPFGRVVALTAAPGKAVDWGVDETRILPFQESVGGRYSLWSSIGFPVALAIGWDEFA
                     QMLEGAAAIDRHFLEAEGAANLPLRAAFADQLYARLRGCQTRAVFAYDERLALLPSYL
                     "
BASE COUNT          550 a         1039 c         1226 g          578 t
ORIGIN      
        1 gatcctcccc gttcgcgggg agggggaccg cacgcgcagc gggcagtgga ggggagtgtc
       61 ctgcaaacgc tgtgcttgga tgagcgcccc ctccgtcagc ctgcggctgc cacctccccg
      121 cgagcgggga ggatcgcgcg cctccatttc gcaattcgtc tcccaggcgg gagccgatgg
      181 cgggacgcta cccgaaccgc ttgaggaatt ccgctgccgc tgcggtttcg gcctcctgct
      241 tgctggagcc ttcggcctcg acctcgccga cgccgtggac atgcaccgag acgcggaagt
      301 gggcggcgtg gtcggggccg gagcgttcga ccaatcggta ttcagggacg cggcggcggt
      361 tgccggcggc ccattcctgc agggccgact tggggtgctt ggactggccg acgcggcctt
      421 cgacggcatc gccccagagt ttgcggacga ggtcgcgcgt gcgatcgaag ccccaggtga
      481 ggaatgaggc gccgagcagg gcctcgacca cgtcgccgag gatattgtcg ctgtcggccc
      541 cgccatcgtc gcgggcctgc ttgccgaggc ggatgtgctg gcccaggccg atcgtgcggg
      601 cgaccttcgc gttgctatcg cggctgacga gggcattgag gcgctgcgag agcttgcctt
      661 catttccggt gccgcgttcg tgcagccatt cggcgatgac gaggccgagg acgcggtcgc
      721 cgaggaattc gaggcgttcg tagttgcgcg gttcgccggt ggagccgtga gtcagggctt
      781 cgacccacag ggcatcggcg gcgatggctt ggccggtcaa ctgctggagg aaagccttcg
      841 tctcatcagc aagcgcggtc accttagatc gtgctcccga tgcggctcca gcgggtcgcc
      901 gtcagccagg tccacggctt gaaccagctc gctgtcccgt tggtcgagaa catcatgatt
      961 tgggccttgc cgacgaggtt ttcctgcggg acgatgccga tgccgccgcc gggcacggcc
     1021 gggaagcggc tgtccatcga attgtcgcga ttgtcgccca tggcgaagaa gtggccttcg
     1081 gggacgatgt agaccccggt gttatcggga tcgaaggtgg gatcgggccg gtaggcatag
     1141 gggccgaagt ccagcacgta gtagctcacc ccgcctggca gggtctcgcg atagcgcgga
     1201 tattggcagg tgggcgagcc atcggcggcg cggcgttcga aggcggggtg atagcagcgg
     1261 gtgttgccca cttccgggat caccgcatcg gccacgcgca ccttgggcac ggccgcgcca
     1321 ttgatgtgga gcacgccgcc ggtcacctgg atctgatcgc cgggcaggcc gatcacgcgc
     1381 ttgatatagt ctgtatcgtt gaacggcggg gccttgaaga tgaccacgtc gccgcgatcg
     1441 ggctggcggg cgaagatccg gccggggatc agcggaaccg agagcggcag cgaatagcgc
     1501 gagaagccgt aggaccactt ggaggcgagc aggtaatcgc cgttttcaag gcgcggcagc
     1561 atcgattcgc tgggaatgtt gaacggcgcg aagatgaagc tgcggaaggc caggacgacc
     1621 agcaccagct tgacgaggaa gacgaagaaa ttctcttcct tctttgcctt ggcgggcttg
     1681 ccgggggtgg aaggttggcc ggaaggcttc gcatcaaggg aagctgattg cgccgggtcc
     1741 ggcgtatggt cggtcatgca aagggtttcc gtggcctcat cgaaccggtg aggttcgtcg
     1801 gcagcggcat agtccacccg ctagcggagg gccagcccca tccggttcag cccggtcgaa
     1861 ggggcagggg aggggcaatg cagaccatca atcgtggagc gagatggcgc ggcgttgctg
     1921 taccacccca aaaaccccct gctctgaaga gaagggggct ttggggctgc cgcgggatga
     1981 gaatcaggcg atggcccagt agagcagcag ggccgaggca aggaactgga agatcacgat
     2041 caccgggctg tgctcgaagt agccctggat cgcggtcatc actgcgagcg agcccatgaa
     2101 caaggctgcg atcaggagca tgtggcggta attcagggtc actgctgtct cgcaaggttg
     2161 agcttgtatt aatgcggtgc tgtgccgccc gggcccgcgg gggcgagcgc cggggcgaca
     2221 cgacttttat ataaattcaa taggttaacg gattcacttc gtttcagttg atacaatggt
     2281 ttgcaacgca gacctatgcg tggcccggca tgcttgcgcg ttgcggggag ttgtttagcc
     2341 aacgcggagc cggtgagtcg caagtccggc ggcgaatcga tggagcatgc cgatgaccca
     2401 agccgcacaa gaccccgtca gcgccgcctg gtccgccctg gccgccctgc cgcgacccag
     2461 cctggcggac ctgtttgccc acggtgaccg gctcggcctg ctcgccacgt cgctcgacct
     2521 gccgggcggc ccgatccgct tcgactggtc gaagacacac ctcgatgaag ccagcctggc
     2581 cgggttcgag cgactggccg aggccaccgg ctttgccgag cggcgccggg cgctgttcgc
     2641 gggggagccc atcaatgcca cggaaggccg cgcggcggag cacatggcgc agcgcggact
     2701 tggcgccgag gccagcgtgg aagaggcggc ctcgctccac gcgcggatgc acatgctggt
     2761 cgaggcgatc cacggcggcg ccttcggcga ggtgcgccac ctgatccaca tcggcatcgg
     2821 cggctcggcg ctggggccgg ccctggcgat cgatgccttg gcgcgggaca atccgatggt
     2881 cgacgtccac gtggtttcca acatcgacgg ctgcgcgctg gagcaggcct ttgccgcctg
     2941 cgatccggcg acgacgatga tcgcggtcgc ctccaagacc ttcaccacca tcgagaccat
     3001 gaccaacgcc cattccgcgc tcgactggct gcgcgagaac ggcgaggccg atcccttcgg
     3061 ccgggtggtc gcgctgaccg ccgcgccggg caaggcggtg gactgggggg tggacgagac
     3121 ccgcatcctg ccgttccagg aatcggtggg cgggcgctat tcgctgtggt cgtcgatcgg
     3181 ctttccggtg gcgctggcga tcggctggga cgaattcgcc cagatgctgg agggcgccgc
     3241 ggcgatcgac cggcatttcc tggaagccga gggcgcggcc aacctgccct tgcgcgcggc
     3301 cttcgccgac cagctctatg cgcggctgcg cgggtgccag acgcgggcgg tgttcgccta
     3361 tgacgagcgg ctggcgctgc tgccgagcta tct
//
LOCUS       DATNBV010000458         2904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4091878,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000458 DATNBV010000000
VERSION     DATNBV010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4091878"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..752
                     /gene="uppS"
                     /locus_tag="VN627_10830"
     CDS             66..752
                     /gene="uppS"
                     /locus_tag="VN627_10830"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HXD03789.1"
                     /translation="MSRSPVIPRHVAIIMDGNGRWAKKRHLPRALGHQRGVEAVRRTV
                     RAARDLGLEALTLYAFSTENWTRPEDEVSALMGLMKKFILADLDEFAAAGVRLKIIGD
                     YKAFAPDLVELIEGALARTAGNTRTTLAVALNYGSMDEIARAARAAAQEGEITPETIA
                     AHLDTADLPPLDLLIRTSGEVRLSNFLLWQAAYAEMLFVDTLWPDFGADDLKAALDEF
                     ARRERRYGGR"
     gene            897..1625
                     /locus_tag="VN627_10835"
     CDS             897..1625
                     /locus_tag="VN627_10835"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXD03790.1"
                     /translation="MTDAPAKKSDLPVRTPSAVVMLAVAGTALWLGGWTWNLLVLAIS
                     GGVLWEFARLVIAFEQRAAMRLAWLTFGLVYVGLAGFTLLLLRSPFYGMGPVLTVAGA
                     VIGVDIGAYFTGRTFGGPKIAPRISPSKTWSGLAGGMAGAALALFVAIGVSRGFGFSP
                     AEYGVLLFGIPFAIIAQAGDFFESWLKRRAGVKDSSRLIPGHGGLFDRVDGLLAVCFT
                     IGLLMLGTVLLTLARGESLLPSPA"
     gene            1630..2781
                     /locus_tag="VN627_10840"
     CDS             1630..2781
                     /locus_tag="VN627_10840"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXD03791.1"
                     /translation="MRTISILGATGSIGASTLDLIRRNRDQWRVVALSANSNAAELAQ
                     LAREFSAELAVVADEACLPALREALAGTEIAAAAGEAALVEAAARPADITVAAIVGCA
                     GLAPTMAAIAQGNTVALANKEALVSAGEVMTAAVAHSGATLLPVDSEHNAIFQCLAGN
                     DLAHVRWITLTASGGPFRDWPAERLAHATPAEAVKHPNWSMGAKISVDSATMMNKGLE
                     YIEAYHLFPVGTERIRIIVHPQSIVHSMVEYRDGSTLAQLGPSDMRVPIASALAWPAR
                     MDTPCAPLDLAAIGELTFRAPDEQRFPATRLAREAAAAGGGVPAVLNAANEVAVAAFL
                     SGKLAFTRIAAQVEETLGRFAPRAPASLDEVIALDAEARARTRALMEPA"
     gene            2810..>2904
                     /locus_tag="VN627_10845"
     CDS             2810..>2904
                     /locus_tag="VN627_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXD03792.1"
                     /translation="MLVGFVLLLGPLVVIHELGHYFVARWCGVKA"
BASE COUNT          406 a         1010 c          967 g          521 t
ORIGIN      
        1 cgagcgcagt cgagacacgg gcgcgcggcc tctcgacttc gctcgaggcg aacggggata
       61 gagggatgtc caggtcccct gttattcccc gccacgtcgc catcatcatg gacggcaacg
      121 ggcgctgggc gaagaagcgc cacctgccgc gtgcgctggg ccaccagcgc ggggtcgagg
      181 cagtgcgccg cacagttcgc gcggcgcgcg acctcgggct ggaggcgctg accctctatg
      241 ccttctcgac cgagaactgg acccgtccgg aggacgaggt atcggccctg atggggctga
      301 tgaagaagtt catccttgcc gatctcgacg agttcgccgc ggccggggtt cggctcaaga
      361 tcattggcga ttacaaggcc ttcgcgcccg atcttgtcga actgatcgaa ggcgcgctgg
      421 cccgcaccgc cggcaatacg cgcacgacac tggcggttgc gctcaactat ggctcgatgg
      481 acgagattgc ccgggcggcg cgggcagccg cgcaggaagg ggagatcacc ccggaaacga
      541 tcgccgccca tctcgacacg gccgacctgc cgccgctcga tctgctgatc cgcacatctg
      601 gcgaggtgcg gctgtcgaac ttcctgctgt ggcaggcggc ctatgccgag atgctgttcg
      661 tcgatacgct ctggccggat ttcggcgcgg acgatctcaa ggccgcgctg gacgaattcg
      721 cgcggaggga gcggcgttat ggtgggcggt aacgagcaaa tcctccccgt ctgcggggag
      781 ggggaccgcc gggacggcgg tgaagggggc gttcaggcag gcgtctcgca tcgcaggacg
      841 gccccctccg tcagccctac ggtctgccac ctccccacga gtgcggagga tatttcatga
      901 ccgacgctcc cgccaagaag tccgacctgc ccgtccgcac tccttcggcc gtggtcatgc
      961 tggcagtggc gggcacggcg ctgtggctgg gcgggtggac atggaacctg ctggtcctcg
     1021 ccatttccgg cggcgttctg tgggagtttg cccgcctggt catcgccttt gagcagcgcg
     1081 cagccatgcg gctggcgtgg ctcacgtttg gccttgtcta tgtcggcctc gccggtttta
     1141 ccctgctgct cctgcgcagc ccgttctatg gcatgggccc ggtcctgacc gtcgccggcg
     1201 ccgtgatcgg cgtcgatatt ggagcctatt tcaccggccg cacgttcggc ggccccaaga
     1261 ttgcgccccg gatcagccct tcgaagacct ggtccggtct tgccggcggg atggccgggg
     1321 cggcgctggc gctgttcgtc gccatcgggg tgtcccgcgg gttcggcttc tcgcctgcgg
     1381 aatacggcgt gctgctgttc ggcattcctt tcgcgatcat cgcccaggcc ggcgatttct
     1441 tcgaatcctg gctcaagcgc cgcgcggggg tgaaggacag ctcccgcctg atccccggtc
     1501 acggcggcct gttcgaccgg gtggacgggt tgctggcagt ctgctttacc atcggtctcc
     1561 tgatgctcgg caccgtgctc ctcacgctcg ctcgcggcga gtctctcctg cccagcccgg
     1621 cgtgaaccca tgcgcaccat ctccattctc ggcgcgactg gttccatcgg cgcttccacg
     1681 ctcgacctga tccgccgcaa tagggatcag tggcgcgtcg ttgcgctctc ggccaacagc
     1741 aatgccgccg aactggcgca gcttgcccga gagttttcgg ccgaactggc ggtcgtggcc
     1801 gacgaggcct gcctgcctgc actgcgcgag gccctggccg ggacggagat cgccgccgct
     1861 gcgggggagg cggccctcgt cgaggctgcc gcgcgcccgg ccgacatcac cgtcgctgcc
     1921 atcgtcggct gcgccggcct tgcccccacc atggccgcca tcgcgcaagg aaacaccgtt
     1981 gccctcgcca acaaggaagc gctggtctcc gccggcgagg taatgaccgc cgctgtcgcc
     2041 cattctggcg cgacgctgct gccggtcgat tccgagcaca acgcgatctt ccagtgcctc
     2101 gccggcaacg atctcgccca cgtccgctgg atcacgctga cggcgagcgg cggcccgttc
     2161 cgggactggc cggcggagcg tctggcccat gccaccccgg ccgaggcggt caagcatccc
     2221 aactggtcga tgggcgccaa gatcagcgtc gattccgcca cgatgatgaa caaggggctc
     2281 gaatatatcg aggcttatca cctgttcccc gtcggtacgg agcgcatcag gatcatcgtc
     2341 caccctcagt ccatcgtcca ttccatggtt gaataccgtg acggctccac gctggcccag
     2401 ctcggcccat cggacatgcg cgtccccatc gcctcggcgc tggcctggcc cgcgcggatg
     2461 gacacgccct gtgcaccgct cgaccttgcc gccatcggcg agctgacctt ccgcgcgccg
     2521 gatgaacagc gcttccccgc cacgcgccta gcccgcgagg ctgccgctgc cgggggcggg
     2581 gtgccggccg ttctcaatgc cgcgaacgaa gtggctgttg ccgcgtttct ctccggtaag
     2641 ctggcgttca cccggatcgc cgcacaagta gaggaaaccc tggggcgctt cgccccgcgc
     2701 gcgccggcct cgctcgacga ggtcatcgcg ctcgatgcgg aggcccgcgc tcgcacccgg
     2761 gcgctgatgg agccggcctg acgtcatgca ttcaccgtcg atcctgatga tgctggtcgg
     2821 cttcgtgctc ctgctcgggc cgctggtggt catccatgag ctggggcact actttgtcgc
     2881 ccgctggtgc ggggtgaagg cgga
//
LOCUS       DATNBV010000459         3121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4378199,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000459 DATNBV010000000
VERSION     DATNBV010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4378199"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VN627_10850"
     CDS             <1..578
                     /locus_tag="VN627_10850"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXD03793.1"
                     /translation="DGGASQFVKVIEALRRNTPDTTIEILTPDFRGKMLPAVEAIVAA
                     GPDVYNHNLETVPRLYPTIRPGARYYASLRLLEEVKRHSPTVFTKSGIMLGLGEERLE
                     VHQVMDDMRCAGIDFLTMGQYLRPTPKHAEVKEFVTPQAFAAYGAIARAKGFLQVASS
                     PLTRSSYHAGEDFAQMRAAREALLARQAARS"
     gene            587..1039
                     /locus_tag="VN627_10855"
     CDS             587..1039
                     /locus_tag="VN627_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HXD03794.1"
                     /translation="MAAIHEVRRLPWSAAQMFDLVADVGRYGEFLPWVVATRVRSNSE
                     SEMVADMLVGFSALREKFTSRVEKHRPDRLVVHYVDGPLKDLDNQWTFRDVAPGECEI
                     EFEVSFSFRNALFQKLANQYLDKAFRKMVAAFEARAAQVYGAGATSAA"
     gene            complement(1069..1575)
                     /locus_tag="VN627_10860"
     CDS             complement(1069..1575)
                     /locus_tag="VN627_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HXD03795.1"
                     /translation="MTESYFPAELIDLARRVVEENAAIGRKVAVAESCTGGLVAAALT
                     EIPGSSVVLDRGFVTYSNEAKQELLGVASDIIDTFGAVSIACAWAMAEGALKRSRADV
                     AVSISGVAGPAGGSDQKPVGTVVFARAVRDPGDPEAGDAEERLFPDTGRSGVRLQAAI
                     CALELLLP"
     gene            complement(1594..2124)
                     /locus_tag="VN627_10865"
     CDS             complement(1594..2124)
                     /locus_tag="VN627_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03796.1"
                     /translation="MTRLSLLAAAALLAVPQVAMAAEPVCLTPREFTAMSTYALPSVI
                     AGTSRACAAQLPDTAFLRRSGGDLAARYAQGKDRAWPEARAAFLKMSMARDPGAAQLF
                     TAMPDDALQQVADAAFAGIVAGQVKPGSCATIDRVVALLSPLPAENTAELIALAAGLG
                     ARSGEARVGKFALCKA"
     gene            complement(2139..>3121)
                     /gene="ispF"
                     /locus_tag="VN627_10870"
     CDS             complement(2139..>3121)
                     /gene="ispF"
                     /locus_tag="VN627_10870"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HXD03797.1"
                     /translation="CAPVIVCIPEGAENIAAQALAGLPVRFVTGGATRQLSVRAGLET
                     LAADAPERVLIHDAARPILPRAVIDRLVAALASASGAIPVLPVVDSLCLADGTPADRA
                     AMRRVQTPQAFRYAEIRAVHDAWNGLAEAGDDAQVAQAAGLSVALVEGDEALHKLTFG
                     SDFAPALQPFRIGTGYDVHRLTEGEELWLCGIKVEHSHGLAGHSDADVAIHALVDALL
                     GAVAAKDIGAHFPPSDPQWKGAASGQFLAHAAALVRGAGYEIGNVDLTIICEAPKIGP
                     HRDAMRRQLAEVLGVDMDAVSVKATTTEGLGAAGRREGIAAQAVATVIRA"
BASE COUNT          509 a         1055 c         1086 g          471 t
ORIGIN      
        1 cggacggcgg cgccagccag ttcgtgaagg tgatcgaggc gctgcggcgc aacacgcccg
       61 atacgacgat cgagatcctc acccccgact tccgcggcaa gatgctgccg gcggtcgagg
      121 cgatcgtcgc cgccgggccc gacgtctaca accacaacct cgagaccgtg ccgcggctct
      181 acccgacgat ccgccccggc gcgcgctatt acgcctcgct gcgcctgctc gaggaggtca
      241 agcgccactc gccgacggtc ttcaccaaga gcggcatcat gctcgggctg ggcgaggagc
      301 gcctcgaagt ccaccaggtg atggacgaca tgcgctgcgc gggaatcgat ttcctgacga
      361 tgggccaata cctgcggccc acgcccaagc atgccgaggt caaggaattc gtgaccccgc
      421 aggcctttgc cgcttacggc gcgattgccc gggccaaggg cttcctccag gtcgcctcca
      481 gcccgctgac ccgctccagc tatcacgcgg gcgaagactt cgcccaaatg cgcgccgcgc
      541 gcgaggcgct gctggccagg caagcggcca ggagctgatc cggaacgtgg cagccattca
      601 cgaagtccgg cgcctgccat ggagtgccgc gcagatgttc gatctggtcg ctgatgtcgg
      661 ccgctacggc gaattcctgc cgtgggtcgt cgccacgcgg gtacgctcca acagcgagag
      721 cgagatggtg gccgacatgc tggttggctt ctcggccctg cgcgaaaagt tcacctcgcg
      781 ggtcgagaag caccggccgg accggctcgt ggttcattat gtcgatggcc cgctcaagga
      841 tctcgacaac cagtggacgt ttcgcgacgt cgctccgggc gagtgcgaga tcgagttcga
      901 ggtcagcttc tctttccgga atgccctgtt ccagaagctg gccaaccagt atctggacaa
      961 ggcgttccgc aagatggtcg ccgccttcga ggcgcgggcg gcccaggttt acggggcggg
     1021 ggctaccagc gcggcctgag cgagcaaggc gtggagcaag cctgcgattc agggcagcag
     1081 cagctccagc gcgcagatcg ccgcctgcag gcgcacgcca gaacggccgg tgtccgggaa
     1141 cagccgctct tcggcgtcac cggcttcggg atcgcccgga tcgcgcacgg cgcgggcgaa
     1201 caccaccgtt ccgaccggct tctggtccga tccgcctgcc gggccggcga ctccgctgat
     1261 cgacacggcc acatccgccc ggctgcgctt gagcgcgcct tcggccatgg cccaggcgca
     1321 ggcgatggaa acggcaccaa acgtgtcgat gatgtcgctg gcgacgccga gcagttcctg
     1381 cttggcctcg ttggaatagg tcacgaaccc gcgatccaac acgaccgagg agccgggaat
     1441 ttcggtcagc gcggcggcga ccaggccgcc ggtgcagctc tccgccacgg ccaccttgcg
     1501 gccgatagcg gcgttttcct caaccacgcg gcgggcgagg tcgatcagct cagcgggaaa
     1561 gtacgattcg gtcatcgaag cgggcccata tgctcaggct ttgcagagcg caaatttgcc
     1621 gacccgcgcc tcgccgctcc tggcgccgag cccggccgcc agcgcaatca gttcggcggt
     1681 gttttccgcg ggcagggggg agagcagggc gactacccgg tcgatcgtcg cgcaggagcc
     1741 tggcttgacc tgcccggcga cgatcccggc aaaggcggcg tctgccacct gctggagcgc
     1801 atcgtcgggc atggcggtga acaactgcgc cgcgccggga tcgcgtgcca tgctcatctt
     1861 gaggaaggcg gcgcgggctt cgggccaggc gcggtccttg ccctgggcat agcgcgcggc
     1921 aagatcgccg ccggagcggc gcaggaaagc ggtgtcgggc agctgcgcgg cacaggcgcg
     1981 gctggtgccg gcgatcacgc tgggcagggc ataggtcgac atcgcggtga attcgcgcgg
     2041 ggtcaggcag accggctcgg cggccatggc cacctgcggg acggcgagga gcgcggcggc
     2101 ggcaagaagt gaaaggcggg tcatcggcaa atccccgttc aggcgcggat cacggtggca
     2161 acggcctggg cggcaatgcc ctcgcggcgg ccggcagcac cgagcccctc ggtggtcgtc
     2221 gccttgacgc tcaccgcatc catgtcaacg cccaggacct cggctagttg ccgccgcatg
     2281 gcgtcgcggt gcggtccgat cttcggtgcc tcgcagatga tggtgagatc gacattgccg
     2341 atctcgtatc ctgccccccg caccagcgcg gccgcgtggg ccaggaactg gccggacgcg
     2401 gcccccttcc actgcgggtc ggagggcgga aagtgcgcgc cgatgtcctt cgccgccacg
     2461 gcgccgagca gggcatcgac aagggcgtgg attgccacgt cggcatcgga atgcccggcg
     2521 aggccgtggc tgtgttcgac cttgatgccg cacagccaca gttcctcccc ctcggtcagg
     2581 cgatgcacgt catatccggt gccaatgcga aagggttgca gggccggtgc gaagtcgctg
     2641 ccgaaggtaa gcttgtgcaa ggcctcgtct ccttcgacca aagccacgga aagtcccgct
     2701 gcctgagcca cttgtgcatc gtcgccagct tcggccagtc cgttccaggc gtcatggacg
     2761 gcgcggattt cggcatagcg gaaggcctgc ggtgtctgga cccggcgcat ggcggcacgg
     2821 tcggccgggg tgccatctgc caggcacagg ctgtccacca ccggcaggac cgggattgcg
     2881 cccgaagcgg aagcgagcgc ggcaaccagc cggtcgatca ccgcacgggg caggatcggt
     2941 cgagcggcgt cgtgaatcag cactcgctcg ggcgcatcgg cggcaagggt ttcgagcccg
     3001 gcgcggaccg acagctgccg cgttgcgccc ccggtgacga agcgcaccgg aagcccggca
     3061 agcgcctgtg cggcgatgtt ttcggcacct tccgggatgc acacgatcac cggcgcgcag
     3121 c
//
LOCUS       DATNBV010000460         6984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_569721,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000460 DATNBV010000000
VERSION     DATNBV010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569721"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VN627_10875"
     CDS             <1..574
                     /locus_tag="VN627_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HXD03798.1"
                     /translation="LAASALGEKAQCLVDLGHHAPNVNIELIVARLIAAGKLAGFHFN
                     DSKYGDDDLDSGSINPHQLFLVFNELAEAETHPRDGFNPSYMIDQSHNVTDPIESMLS
                     SAEAIAQCYAKASLVDRAALHGAQEANDTMMAFQALRRAYNIDVAPILAKARVEAGGA
                     FDVLEVYRESRWRERKAQERKAVGLGAGIV"
     gene            713..1264
                     /locus_tag="VN627_10880"
     CDS             713..1264
                     /locus_tag="VN627_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HXD03799.1"
                     /translation="MGLTLGGGQVTPREEAALIARIDGAGLAPFRFHGWLGKRLTASF
                     GHGYDFGQGRLVEAPPMPRWLLSLRQRLALWAGLDPASFVQALVIRYDPGAGIGWHRD
                     RPQFDHVIGLSLGHATPLRLRRRLPDGKFERRMVPLEPRSAYSLTGPARWEWQHAILP
                     VAKPRWSVTFRSLRGAQPSTWGQ"
     gene            complement(1270..3588)
                     /locus_tag="VN627_10885"
     CDS             complement(1270..3588)
                     /locus_tag="VN627_10885"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXD03800.1"
                     /translation="MVKRLWLGLSMTTMLAGSLQAQSGRTVLPVPPAPFSGTVAENVL
                     DASAPAQQAVQAPAGAPNVLLIMSDDVGFAMSSTFGGPVPTPNYDRLARTGQRYNRFH
                     TTGICSPSRAALLTGRNHHNAGTGYLSDQVTAFPGYGGRILPETATIAQVLRLNGYNT
                     AMFGKDHNVPAADRSEAGPFDSWPTGRGFDYFYGFIGGDTDQYSPPLFRGIQRAEPAA
                     DDGKLLDRRLTDDIIRWVHNQKAGAPNKPFMVYFAPGSTHAPHQAPSDYIARFKGRFD
                     GGWDKLREETWRRQLAMGIIPKGTRLTARPAEIPAWDSLTPGQKAFAARTMEVAAAQL
                     AYQDEQVGRLLDELQRMGQFDNTLVAAILGDNGASAEAGPAGTINELRSLFSRDEREA
                     WMEANIDRLGGPMTYESYPAGWSLAMNTPLRWTKQYASFLGGIRNGMILSWAGHVAHP
                     GAVCSQFGHLVDIAPTILEASHLPAPDVVLGAKQKPLDGQSLLTSLTTACDPAHPRTQ
                     YFEIGGKIGLYHDGWFLSGEDGRMAWQNLPPTGPRPQVTWSLYNLASDFSQATDLASR
                     EPQRLREMQALWQQEASRNNVFPLDHRFAAARSWMGATKGPRHYDFWGKDVSIPAISA
                     PALVGRSFTVSADLKLDSAAASGVVLAYGSRFGGWSLLLDHGRPSLVWARSTDPEEIR
                     AVTATSALPKGASRLEMRFAVEKPGGPATVTLSSGGAELARLALPQSILVPAGTIETL
                     DVGRDLGVPVTDYATPHGAIEGDIPHVTIDFD"
     gene            3758..4375
                     /locus_tag="VN627_10890"
     CDS             3758..4375
                     /locus_tag="VN627_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HXD03801.1"
                     /translation="MALPLEPFLPWLDMAGRAVFAASGALAAARKRLDVGACFFALIT
                     ATGGGTLRDLLIGAPVFWMHSPMPIVLCAMVAIAAWVVPLRWWPQRALEWFDGLGLAA
                     YAVYGAGKALTYGIAPLPAAVAGIITACLGGIVRDVTAGVPSVLMRHELYVTAAACAA
                     FLFVALSALGGVVAQWAALVSVGLGFLIRAAAIQWGLSLPPHRGH"
     gene            complement(4376..5221)
                     /locus_tag="VN627_10895"
     CDS             complement(4376..5221)
                     /locus_tag="VN627_10895"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD03802.1"
                     /translation="MAIIDAQVHIWAADSPQRPWPSAGAGGRTTAVHGASFGADQLIA
                     AMDGSGIDAAVLIPPSWEGERTDLVEAAHLAWPERLAFMARLDLADEPRARAEFARIA
                     ALPGALGFRFLLGSDIVLDQLPGLDWLWAGIAEAGLSIMAAPRGYFAELAQVARAHPD
                     LRITIDHLGARAYKKGAEAFAGLDRMLELASLPNVAVKASGLPEYSEVPPPHADIGPR
                     VRRVFEAFGPDRLFFGTDLTRQEGGYPALLALFDSFDWLRGEDRRKVMGDALADWLRW
                     DRAPR"
     gene            complement(5223..6269)
                     /locus_tag="VN627_10900"
     CDS             complement(5223..6269)
                     /locus_tag="VN627_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03803.1"
                     /translation="MTDQTLLDIPADDDFEAINEQFYRRRMTDGLPIIPPTRARVDRM
                     LAATRRKPDAALGAIGPKFGVATVEKVAVNAVMAGCAPEYLPVVIAALEAMLTPEFNL
                     YGINTTTHPCNPMIVVNGPIREKLRINYGYNCMGGGWRANATIGRAVRLCMINIGGGI
                     AGDGDRATHGTPAKISFCLGENEAASPWEPLHVRRGFRADESTVTVHGSEAPHEVNNH
                     TNFDALGVLKTLASTAATLGSNNSYLNTGEVAVCLGPEHAETIAREGWSIADVQDYLY
                     VHARNRGSDMAVLDRIGNKSTKRWDPRDPTATVPLVMRPEDFLIFVAGGAGKHSMIMP
                     SFGYTRAATVKIED"
     gene            complement(6305..>6984)
                     /locus_tag="VN627_10905"
     CDS             complement(6305..>6984)
                     /locus_tag="VN627_10905"
                     /inference="COORDINATES: protein motif:HMM:NF041046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HXD03804.1"
                     /translation="LGANQTSGANAMATAVSASTLMQNADMLLDPVGYPGSDVNPIAA
                     RLDGLAGKTIGLLDNTKNNADLLLDEIGRVLLADHGVKAIVSRRKVGSSPGAPEDMLD
                     ELAACDAVVNAYGDCGSCTSWCVHDSVVLEKRGIPTATVNTDEFVSLGQMDAISLGLP
                     GLPIVVVKHPMGDLPEPTVRERARGAVDQVVALLTTDARELEAQYTGKFLRESEKLRS
                     GTLACPI"
BASE COUNT         1046 a         2288 c         2451 g         1199 t
ORIGIN      
        1 cctcgccgcg agtgcgctcg gcgagaaggc gcaatgcctc gtggacctcg gtcaccatgc
       61 gccgaacgtc aatatcgaac tgatcgttgc gcggctgatc gccgccggca agctggccgg
      121 cttccacttc aacgacagca agtatggcga cgacgacctc gattcgggct cgatcaaccc
      181 gcaccagctg ttcctcgtgt tcaacgaact ggccgaggcc gagacccatc cgcgggacgg
      241 cttcaacccg agctacatga tcgaccagtc gcacaacgtg accgatccga tcgaatcgat
      301 gctctcttcc gccgaggcga ttgcccagtg ctacgccaag gcgagtctcg tcgaccgggc
      361 ggcgctgcat ggcgcgcagg aagcgaacga cacgatgatg gccttccagg cgctgcgccg
      421 ggcctacaat atcgacgtgg ccccgatcct cgccaaggcg cgggtggaag ccggcggagc
      481 gtttgacgtg cttgaggtct atcgcgagag ccggtggcgc gagcgcaagg cgcaggagcg
      541 caaggcggtc gggctgggcg ccgggatcgt ctgacccggg aaggcgcgcg cttcgcccgg
      601 caagctcacg gcgggaccta tgtgggcttg ccggtctgat gccggctccc atatcgggcc
      661 ggtgcgtggc aaccagttga acctgttcga taatcctgca tccgatctgc ccgtggggct
      721 gactctcggc ggcgggcagg tcaccccgcg ggaagaagcg gcgctgattg cccgaataga
      781 cggtgcaggc cttgccccgt tccgtttcca cggatggctt ggcaagcggc tgacagcttc
      841 gttcggccac ggctacgatt tcgggcaagg caggctggtg gaggcgccgc ccatgccccg
      901 atggctgctg tctttgcgcc agcgattggc attgtgggct ggcctcgacc cggcaagctt
      961 cgtccaggcg ctggtgatcc gctacgatcc gggcgcgggg atcgggtggc atcgcgaccg
     1021 gccccagttc gatcatgtga tcggcctctc gctcggccat gccacgccgc tgcggctgcg
     1081 gcgccgcttg cccgatggca agttcgagcg gcgcatggtc ccgctggagc cgcgctcggc
     1141 ctactcgctg accgggcccg cgcggtggga gtggcagcac gccatccttc cggtggccaa
     1201 gccgcggtgg tcggtgacgt tccggagctt gcggggcgct cagccgagca cttggggcca
     1261 gtagaggcct cagtcgaaat cgatggtgac gtgcggaata tcgccttcga tcgcgccatg
     1321 gggggtggcg taatccgtca ccggcacccc gaggtcgcgg cccacgtcga gcgtctcgat
     1381 ggtcccggcg ggtaccagta tgctctgcgg cagggcgagc cgggcgagtt cggccccgcc
     1441 tgacgagagc gttaccgtgg ccggtccgcc gggcttctcc acggcaaagc gcatctcgag
     1501 gcggctcgct cccttgggca gggcggatgt tgcggtgacg gcgcggatct cctcgggatc
     1561 ggtcgaccgc gcccagacca gacttggccg gccgtggtcg agcaacaggc tccagccgcc
     1621 gaaccggctg ccataggcca gtaccacccc cgaagctgca gccgaatcca gcttgaggtc
     1681 ggcgctgacg gtgaacgagc ggccgaccag cgccggggcg gaaatcgccg gaatgctcac
     1741 atccttgccc cagaaatcgt aatgcctggg ccctttggtc gcgcccatcc acgagcgggc
     1801 cgcggcaaag cggtgatcga gcgggaagac gttgttgcgt gaggcctcct gctgccacag
     1861 cgcctgcatt tccctgagcc tctgcggctc acgcgaggca aggtcggtcg cctgcgagaa
     1921 atcgctcgcg aggttgtaga gcgaccaggt gacctgcggg cgcgggccgg tgggcggcag
     1981 gttttgccag gccatccgcc cgtcctcgcc cgataggaac cagccatcgt ggtagagccc
     2041 gatcttcccg ccgatctcga aatactgggt gcgcgggtgc gcggggtcgc aggcggtggt
     2101 caggctcgtc agcaggctct gcccgtccag cggcttctgc ttcgcgccca gcaccacatc
     2161 gggcgcgggc agatgactgg cctccaggat cgtgggggcg atgtcgacca ggtggccgaa
     2221 ctgactgcac accgcgcccg ggtgggccac atggcctgcc cacgagagga tcatgccatt
     2281 gcggatgccg ccgaggaacg aggcatattg cttggtccag cgcagcggcg tgttcatcgc
     2341 cagagaccag ccggccggat aggattcgta ggtcatcggc ccgccgagcc gatcgatatt
     2401 ggcctccatc caggcctcgc gctcatcgcg cgagaacagc gagcgcaatt cgttgatcgt
     2461 gcccgccggc ccggcctcgg cactggcgcc attgtcgccg aggatcgcgg cgacgagagt
     2521 gttgtcgaac tggcccatcc gctgcagctc gtcgagcagg cggccgacct gctcgtcctg
     2581 ataggcaagc tgcgccgccg caacttccat cgtccgcgcg gcgaaggctt tctggcccgg
     2641 cgtgaggctg tcccaagcgg gaatttccgc aggacgcgcg gtcagccggg tgcccttggg
     2701 gatgatcccc atggccagct gccgccgcca ggtctcctcg cgcagcttgt cccatccgcc
     2761 atcgaaacgg cccttgaacc gggcgatgta gtcgcttggg gcctgatgcg gcgcatgggt
     2821 cgatcccggt gcgaaataga ccatgaacgg cttgttcggc gcgcccgcct tctggttgtg
     2881 gacccagcgg atgatgtcgt cggtcaagcg gcggtcgagc agcttgccgt cgtcggcggc
     2941 gggttcggcg cgctggatgc cgcggaagag gggtggcgaa tactggtcag tatcgccgcc
     3001 gatgaacccg tagaaatagt cgaacccgcg ccccgttggc caggaatcga acggcccggc
     3061 ctcgctgcga tcagccgccg ggacgttgtg atccttgccg aacatcgccg tgttgtagcc
     3121 attcagccgc aggacctgcg cgatcgtcgc cgtttcgggc agaatgcgtc cgccatagcc
     3181 ggggaaggcg gtgacctggt cggacaggta accggtgccc gcattgtggt ggttgcgtcc
     3241 ggtcagcaga gcggcccggc tgggcgaaca gatgccggtc gtgtggaagc ggttgtatcg
     3301 ctggccggtg cgggccagcc ggtcgtaatt gggcgtcggc accggtccgc cgaaggtgga
     3361 cgacatggca aagcccacat cgtcactcat gatcaggagc acgttgggcg cgccggcggg
     3421 ggcctgcacg gcctgctgtg cgggggcgct ggcatcgagc acgttctcgg cgacggttcc
     3481 gctgaatgga gcagggggca cgggcagtac cgtccgcccg ctctgggcct ggagactgcc
     3541 ggccagcatg gtcgtcatcg acagccccaa ccacagccgc ttcaccatcg ctatctcccc
     3601 cggggccaag cccttgttat gcctggtgat aggctttgcg ggcccgatgc gtccaatgca
     3661 aagcgagcgt taattcgata attattggtt atttgggcca tcctgtgcgg agacttccgg
     3721 cctagcgaag tcaggcgtgc cgccttatgg agcgcccatg gcgcttccgc tcgaaccgtt
     3781 cctgccgtgg ctggatatgg ccggcagggc agtgtttgcg gcatcgggcg cgctggcggc
     3841 ggcgcgcaag cggctggatg tcggcgcctg cttcttcgcc ctgatcaccg cgaccggcgg
     3901 ggggaccttg cgcgacctgc tgatcggggc cccggtgttc tggatgcaca gcccgatgcc
     3961 gattgtgctg tgcgcgatgg tggcgatcgc cgcctgggtc gtgccgctgc gctggtggcc
     4021 gcaacgggcg ctcgaatggt tcgacgggct ggggctggcg gcctatgcgg tttacggcgc
     4081 gggcaaggcg ctgacctacg gcatcgcccc gctgcccgct gcggttgccg gcatcattac
     4141 cgcctgcctt ggcggcattg tccgcgatgt gacggccggg gtgccctcgg tgctgatgcg
     4201 ccacgagctt tatgtcacgg ctgccgcctg cgccgcattc ctgttcgtcg cgctgtctgc
     4261 actggggggc gtggtcgccc aatgggcggc cctcgtcagc gtcggcctgg ggttcctgat
     4321 ccgcgccgcg gcaatccagt ggggcctgtc gctgccgccc caccgcgggc actgactagc
     4381 gcggcgcacg gtcccagcgc agccaatccg ccagcgcatc gcccatgacc ttgcggcgat
     4441 cctcgcccct tagccagtcg aagctgtcga acagcgcgag cagggccgga tagccgccct
     4501 cctgccgggt aaggtcggtg ccgaagaaca gccggtccgg cccgaaggcc tcgaacaccc
     4561 ggcgcacgcg cgggccgata tcggcatggg gtgggggcac ttcgctgtat tccggcaggc
     4621 ccgatgcctt gaccgccacg ttgggcaggc tggccagttc gagcatccgg tcgagaccgg
     4681 caaaggcctc cgcgcccttc ttgtaggctc gggcgccgag gtgatcgatc gtgatccgca
     4741 gatcggggtg agcgcgcgcc acctgcgcga gctccgcaaa atagccgcgc ggcgcggcca
     4801 tgatcgagag acccgcttcg gcgatgccgg cccacagcca gtccagcccc ggcagctggt
     4861 cgagcacaat gtcgctgcca agcaggaagc gaaagccgag cgcgccgggc agcgccgcga
     4921 tgcgtgcgaa ctcggcgcgg gcgcgcggct cgtcggcaag gtcgagccgg gccatgaagg
     4981 ccagccgctc gggccaggca aggtgggccg cttcgacaag gtccgtccgc tcgccctccc
     5041 aactgggcgg aatcagcacg gccgcatcga tgcccgatcc atccattgcc gcgatcagct
     5101 ggtctgcgcc aaacgacgcc ccgtgcaccg ccgttgtccg cccgcccgca ccggcgctgg
     5161 gccatggccg ctgcgggctg tctgcggccc agatgtggac ctgggcgtcg atgatcgcca
     5221 agtcagtcct cgatcttcac cgtggccgcg cgggtatagc cgaagctggg catgatcatg
     5281 ctgtgcttgc ccgcgccccc ggcgacgaag atcaggaaat cctccggccg catcaccagc
     5341 ggcaccgtgg cggtgggatc gcgggggtcc cagcgcttgg tcgacttgtt gccgatgcgg
     5401 tcgagcaccg ccatgtcgct cccccggttg cgcgcgtgga catagagata gtcctgcacg
     5461 tcggcgattg accagccctc gcgggcaatc gtctcggcgt gttccggccc caggcacacg
     5521 gccacttcgc cggtgttgag ataggaattg ttgctgccga gcgtcgccgc cgtggaggcg
     5581 agcgtcttga gcacgccgag cgcgtcgaaa ttggtgtggt tgttgacctc gtgcggcgct
     5641 tccgagccat ggacggtcac cgtgctctca tcagcccgga agccccggcg cacgtgcagc
     5701 ggctcccacg gcgaggccgc ctcgttttca cccaggcaga acgaaatctt ggccggcgtg
     5761 ccgtgggtcg cgcggtcgcc gtcgccggcg atgccgccgc cgatgttgat catgcacagg
     5821 cggaccgccc ggccgatggt tgcattggcg cgccagccgc cccccatgca gttgtagcca
     5881 tagttgatcc gcagcttttc gcggatcggc ccgttgacga cgatcatcgg gttgcagggg
     5941 tgggtcgtgg tgttgatgcc gtagagattg aactcgggcg tcagcatggc ttcaagcgcg
     6001 gcgatcacca ccggcaggta ttccggggca cagcccgcca tgaccgcgtt gacggccact
     6061 ttctcgaccg tcgccacccc gaatttgggg ccgatcgcgc ccagcgccgc atcgggcttg
     6121 cgccgggttg ccgccagcat ccggtcgacc cgggcccggg tgggcgggat gatggggagg
     6181 ccgtcggtca tccggcggcg atagaactgt tcgttgatcg cctcgaaatc gtcgtcggcg
     6241 gggatgtcca gcagggtctg gtcggtcatg gggcggtttt cgctctgaga gggagagggg
     6301 cgggtcagat cgggcaggcg agagtgccgc tgcgcagctt ctcgctctcg cgcaggaact
     6361 tgccggtata ttgcgcctcc agctcgcgcg cgtcggtggt gagcagggcc accacctggt
     6421 cgaccgcgcc gcgcgcccgt tcgcgtacgg tcggctcggg caggtcgccc atggggtgct
     6481 tgacgacgac gatcggcagg ccgggcaggc cgagcgaaat ggcgtccatc tgcccgaggc
     6541 tgacgaactc gtcggtattg accgtggccg tgggaatgcc gcgcttttcg aggacgacgg
     6601 aatcgtggac gcaccagctg gtgcacgaac cacaatcgcc ataggcattc accaccgcgt
     6661 cgcaggcggc cagctcgtcc agcatgtctt ccggggcccc gggcgaggag ccgaccttgc
     6721 gccgcgaaac gatcgccttc acgccatgat cggccaggag gacgcggccg atctcgtcca
     6781 gcaggaggtc ggcgttgttc ttggtattgt cgagcaggcc gatggtcttg ccggcaaggc
     6841 catcgagccg cgcggcaatc gggttcacgt cagacccggg gtaacccacg gggtcgagca
     6901 gcatgtccgc gttttgcatg agggttgagg cggagacggc ggttgccatg gcgtttgctc
     6961 cagatgtctg attggctccg aggt
//
LOCUS       DATNBV010000461         2519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1834931,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000461 DATNBV010000000
VERSION     DATNBV010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834931"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VN627_10910"
     CDS             complement(<1..235)
                     /locus_tag="VN627_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HXD03805.1"
                     /translation="MTGEAVTVEVASRVGALPADQWDALTGGNPFVSHAFLSALEDSG
                     SVGSRSGWDPAPLVVKRADGTLAGAIPAYLKSHS"
     gene            complement(232..1005)
                     /locus_tag="VN627_10915"
     CDS             complement(232..1005)
                     /locus_tag="VN627_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HXD03806.1"
                     /translation="MRLSLLAPLDRWLAPAPPADRVAWLRKACFAHRGLYGHGLAENS
                     LGAAEAAIAKGLGIECDVQRSSDGQAVVFHDWELDRLTAERGMAGDRSAAQLGALTLS
                     GTSDTIPTLRHLLDVVAGRVPLLIELKSRRDRRFGALCLAVRRVLEGYRGPHAIMSFD
                     PRITHWFAVNSPQTVRGLVVTEENDKALPGRLRRHFALWHARPDFLSYDIRDLPGRFP
                     AAQRRRGLPLVTWTIGTPELLARSRACADAPTVEGAGVP"
     gene            complement(977..1447)
                     /locus_tag="VN627_10920"
     CDS             complement(977..1447)
                     /locus_tag="VN627_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXD03807.1"
                     /translation="MTEPEIERRLAALGLELPAPAAPVAAYVPVVVAGGLAHVSGQLP
                     FIDGRLVTGRLGEDLSVEDGAAAARACGLMILAQLKAALGSLERVERVVKLGAFVNST
                     GAFVDQPKVANGASELMAAVFGDAGKHARSAVGVPVLPLGAAVEVDAIVAVSPA"
     gene            complement(1440..2072)
                     /locus_tag="VN627_10925"
     CDS             complement(1440..2072)
                     /locus_tag="VN627_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXD03808.1"
                     /translation="MSRPLVISDCDEVLLHMIAPYREWLGETQGITFRMDTHNFVDAM
                     RWADTGEPVEPSEIWRLLNAFFETEMSRQTPIAGAVEAMQALTQVADVVVLTNVGEQH
                     QANRTRQLASHGLDLKVYTNLGPKGPALRLILDEYRPSHAAFIDDIAHHHNSVAEIAP
                     EVTRLHFCGEPQLAPMITCAHAAGHAHARIDDWASALPWLLARLQGAPHD"
     gene            complement(2069..2347)
                     /locus_tag="VN627_10930"
     CDS             complement(2069..2347)
                     /locus_tag="VN627_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 family protein"
                     /protein_id="HXD03809.1"
                     /translation="MTILREPSSPADPQALALGALGWLLQSESRAERLLSLTGLTPDA
                     LRAGIGDPAVLAAVLEFLAAHEPDLVGAAEALGVAPAVLVGAARELQA"
     gene            2407..>2519
                     /locus_tag="VN627_10935"
     CDS             2407..>2519
                     /locus_tag="VN627_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HXD03810.1"
                     /translation="MAKRILVVEDNDLNRKLFCDVLKSQGFAVEPVGDGRQA"
BASE COUNT          447 a          810 c          889 g          373 t
ORIGIN      
        1 ggctgtgcga cttcaggtaa gcgggaatgg cgcccgccag ggtaccatct gcccgcttca
       61 ccaccagggg cgccggatcc catccactgc gcgaacctac gctgcccgaa tcctccagcg
      121 ccgaaaggaa ggcatggctg acgaaagggt tgccgccggt cagcgcatcc cattgatcgg
      181 ccggtagtgc cccgacgcgt gacgcgacct cgaccgtaac cgcttcgccg gtcacggtac
      241 gccggctccc tccacggttg gcgcatccgc gcaggcccgg gaccgcgcga ggagttctgg
      301 ggttccgatc gtccaggtca ccagtggcag gccgcggcga cgctgcgctg cggggaaacg
      361 tcccggcaag tcgcgaatgt cgtaggagag gaaatcgggt cgggcatgcc acaaggcgaa
      421 atggcggcgc aggcggcccg gcaaggcctt gtcgttttcc tcggttacaa cgaggccgcg
      481 cactgtttgc ggcgaattga cggcgaacca gtgcgttatc cgcgggtcga agctcatgat
      541 cgcatgcggg ccgcgatagc cctcgagcac gcgccggact gccaggcaca gggcgccaaa
      601 gcggcggtcg cgccgcgact tcagttcgat cagcaaggga acccggccgg ccaccacgtc
      661 aagcaaatgc ctgagagtgg ggatggtgtc cgaagtgccg gacagagtca gtgcgcccaa
      721 ttgggcagcg ctgcggtctc ccgccatgcc ccgttcagcg gtcagccgat cgagctccca
      781 gtcgtggaag acgactgcct gcccgtcgct ggagcgctgg acgtcgcatt cgatgccgag
      841 ccccttggca atggccgctt ctgccgcccc gagcgagttc tccgccaggc catggccata
      901 caggccgcga tgggcgaagc aggccttgcg cagccacgcg acgcgatcgg cgggcggcgc
      961 cggcgccagc cagcggtcaa gcggggctaa cagcgacaat cgcatcaacc tccacggcag
     1021 cgccgagcgg caggaccggt actccaaccg cgctgcgggc atgcttgccg gcatcgccga
     1081 acaccgccgc catcagctcg gaagcgccgt tggcgacctt gggctgatcg acgaaggcgc
     1141 cggtggaatt gacgaaagcg ccgagcttca ccacgcgctc gacccgttcg agcgagccga
     1201 gggccgcctt cagctgggcc aggatcatca ggccgcaggc gcgggcggcg gcggcgccat
     1261 cctcgaccga gaggtcttcc ccgagccggc cggtgaccag ccgcccatcg atgaacggca
     1321 attggcccga aacgtgggcg aggccgcccg cgacgacgac gggcacgtag gcggcgaccg
     1381 gcgccgccgg agcgggcagc tcgagcccga gagcggcgag acgccgttcg atttcaggtt
     1441 cagtcatgcg gggctccctg caaacgggcg agcagccacg gcaaggcgct ggcccagtca
     1501 tcgatgcggg catgtgcatg gcccgcagcg tgggcgcaag tgatcatcgg ggcgagttgc
     1561 ggctcgccgc agaagtggag gcgggtcacc tcgggggcaa tctcggccac ggaattgtga
     1621 tggtgtgcga tatcgtcgat gaaggcggca tgggacggcc ggtattcgtc gagaatcagg
     1681 cgcagcgccg ggcctttggg cccgagattg gtgtaaacct tgagatcaag gccgtggctg
     1741 gcgagctggc gggtgcggtt ggcctggtgc tgttcgccga cattggtcag cacgacgacg
     1801 tcggccacct gtgtcagggc ctgcatggcc tcaaccgcgc cggcaatcgg ggtctgccgg
     1861 gacatctcgg tttcgaagaa ggcattgagc aggcgccaga tttctgacgg ctcgaccggc
     1921 tcacccgtat cggcccagcg catcgcatcg acgaagttgt gggtgtccat gcggaaggtg
     1981 atgccctggg tctcaccaag ccattcgcgg tatggcgcga tcatgtgcag cagcacttca
     2041 tcgcaatcgg agatgaccag cggacggctc atgcctgcag ttccctggcg gcgccgacca
     2101 gcactgccgg cgcgacgccg agcgcctcgg cggcacccac gagatcgggt tcatgcgcgg
     2161 cgaggaattc cagcacggcg gcgagcacgg ccggatcgcc gatcccggcg cgcaaggcat
     2221 cgggcgtgag accggtgagc gacagcaggc gttcggcccg gctctcgctc tgcaggagcc
     2281 agccgagcgc gccgagggcc agcgcctggg gatcagcggg ggaagatggt tcgcgaagaa
     2341 ttgtcaggga ctttccgagc gcatagagat cgctcgatcc atcccggctc ggcgacagga
     2401 ctgcaagtgg caaagagaat cctcgttgtc gaggacaacg atctcaaccg gaagctcttc
     2461 tgcgatgtgc tgaagagcca gggctttgcc gtcgagccag ttggcgatgg caggcaggc
//
LOCUS       DATNBV010000462        10456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2463396,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000462 DATNBV010000000
VERSION     DATNBV010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10456
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2463396"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VN627_10940"
     CDS             <1..351
                     /locus_tag="VN627_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXD03811.1"
                     /translation="AFYTKALGMKRVLRVSRPQDPYIEDAYSFSGDPLAAEPLVILVQ
                     HNDRKTAPPPSAIVLGVHVADAHAAAAKVRAAGYAVLREPPPEDHGAKLATIAKDPDG
                     NTIELVQLDIARLR"
     gene            417..1193
                     /locus_tag="VN627_10945"
     CDS             417..1193
                     /locus_tag="VN627_10945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD03812.1"
                     /translation="MRDRLAGKRIIVFGSATGIGAATVRRLVEEGARVCAADINLAGA
                     TQVAAEAGGDTFAVYVDIADEASVNAAVETAVGRLGGLDGAHINAADLRVIMEDSDAL
                     ALDLAVFDRTIAVNLRGHLLCTRAVLPHLLRNSQSAVVYTSSGSAHGAEPARPCYAMA
                     KAGINALMRHVAARWGKEGLTANVLAPGFTVTGELKAQMEANAEEAVKWSSYYMDRTP
                     HTRLGESEDHAGVVAMLLSDDGRWINGTIIDVNGGSLMRG"
     gene            complement(1197..2318)
                     /locus_tag="VN627_10950"
     CDS             complement(1197..2318)
                     /locus_tag="VN627_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXD03813.1"
                     /translation="MINPGGMVSFLDRIQGDKMASSIQPASWPTAESMVRHVVALYER
                     EVGSAQDLAAELQIRCNAHAFLDGRELLSHTDLQRLYTRAVSHLSRNAQATEGRLPLR
                     PVDWKVILFGLEGGSTLRESIERAGDCMEAIDGRCGLMSLHIRGDLVELRFETLRRQR
                     TATNCFIDLTGILQMHAELSWLIGQSLPIIEFALDYPAAVYRTLGLPRLPFPVHPEAG
                     WSGFALSASYLDYPVVRTTGERGQTSAPASLALLDPNDIPAQTAALPKVRAIAIEELR
                     RHSRLPSFEEIVGRFGCSSATLRRWLASEGASYREIRNSCRREITLDLLSRTDFSIEE
                     ISRRGDFSDSDAFRRAFREWTGETPRSYRAQIRSPRPAQ"
     gene            2449..3726
                     /locus_tag="VN627_10955"
     CDS             2449..3726
                     /locus_tag="VN627_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03814.1"
                     /translation="MSKKSAAALSGMALVCLAAVGLHAADAPTGGWPGTRTFVLNGLF
                     VGGGGDPKGCPAQSPGNFDVYLASLPADQREALSPPGKRGDLQKQMWQHFGFRRMALW
                     PGDFGGRAEVAKLPPDVKLHQRPSPEQLRAIAELNGFPKGRGSLAYQDQTLAYSSCTN
                     PYDFPELARGYVPYQGKISTGMNLDGKGGAQNFSSPDGEKGIDNQLWRAIGCYKQSQE
                     MNDPALQEKVMLSARSPTLIEVSGIDDPRNDPDVTVRVLTGADPLVRDGAGKPLARAS
                     YSLDPDPALVNVTHGRIVNGELTTDPFDLRFVFREQIIDNLRLVRGAVVRARFKPDGN
                     LDGGIYGYTTVSSFYDMVEHMTQDGADNVGLSCVGVRQALDRYADGYRDRATGKFTAI
                     SSEMRFHAVPAFVTGTVPGGALGGGSATARAMR"
     gene            3723..5393
                     /locus_tag="VN627_10960"
     CDS             3723..5393
                     /locus_tag="VN627_10960"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HXD03815.1"
                     /translation="MSRAASRILLAAAGALAGFGILSSSASFGGSAATSVPQPSVRRL
                     TESQYRAAIAEAFGPEIEVVGRFEPDMRVGGLAAVGASSVSITPAGFEQYDAIGRHVA
                     EQVFAPAARSRLVGCGPDSGDPAGARCAAGFVSRVGLKLFRRPVAPREVQAAVARTLA
                     SAQRLGSFDAGLQATLVGMLTSPDFLFRIDLPAPHGLAGTIDGYSKASRLSFLLWNAP
                     PDSALLEAAGKGLLDSPDGLGAQVDRMIASPRFVDGVRAFFDDFLQLRDMETLSKDGL
                     IYPNFSTTVAAASREQVLRTITDLLVEHKGDYRDLFTTRRFAMTRALGPIYNVPVSGS
                     GWSMYEFPEGDPRIGLLTQAGLLALHSHPGRTSPTLRGKSLRETVLCEQVPSPPANVN
                     FSVVQDVNNVSLKTTRQRLEAHLDDEECASCHKATDGLGLSLEQFDGAGQFRSTEHDV
                     PIDTSGKFDKAVFAGVPALARLMHDSPKVSQCVVSTAWRYANGRDAGPADRPELDRLH
                     ASFAASGYRFIDLMRTIALDSQFYAMPRIPQARPAATARADSKSQDRS"
     gene            5390..6742
                     /locus_tag="VN627_10965"
     CDS             5390..6742
                     /locus_tag="VN627_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HXD03816.1"
                     /translation="MTSPLSRRSFLMRGALGGAAVVVGVPLLDLFLDDSGTAFAASVG
                     GGRLPVRFGTWFWGCGMIPERWVPKAAGTGYDLPPQLAPIKPVQEHVSVLSGYDVMLD
                     GKANLPHISGNTAVRTGEASDTWQGISGPTFDVLISDAIGSGAYFRALDLSLDGNPQT
                     SYSFRNGASMNAAIPSPVELYQKVFGLDFHDPNTADFKPDPRYMVRRSVLSGVTEQRR
                     ALLSRVGAADRARLDQYFTSVREMEDKLALQLQKPPPAEACVRPDAPAAVRGDITDLG
                     VRKANHKLMTQLLGMALACNQTRAFNMNFDTAAAELRRAGDTTGYHQSTHEELVDRSV
                     GYQPTVDKFATGSMEAWADFVSILSGIREGDGSLLDNLLVVAHSEVSFAKNHDVTGIP
                     VMLAGRAGGKVKQGQHIKGAGEPITRVGLTAQQVMGVPVDSWGRGAMRSNRAIGEILA
                     "
     gene            6769..7233
                     /locus_tag="VN627_10970"
     CDS             6769..7233
                     /locus_tag="VN627_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXD03817.1"
                     /translation="MKHMRWAAAGLVLVAGAGAAVAAAPAVIAAIHARQASFKEMGGD
                     FKAISDEIKSGSPDWPAVRRSAHDLSARAPGQERLFVRGSGAEAGVRTRALPLIWADP
                     AGFARANADLVRAAAVLDAAAQRGDLAGLINARAMAGATCKGCHDKYRATDD"
     gene            complement(7285..7986)
                     /locus_tag="VN627_10975"
     CDS             complement(7285..7986)
                     /locus_tag="VN627_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HXD03818.1"
                     /translation="MKKALIIRHVPYEGVAGFRQPIEAAGYDVDRIDVADPAFASVDL
                     AEPDLLIMMGGPMGVYEQDQHPWIACQLRRLAPRLEADRPTLGVCFGAQMIAAALGAR
                     VYPGPVKEVGFHPVSIAPQHDAAFMRHLREVPVLHWHGDTFTLPGGAQLLASSHLYDH
                     QAFRYGRNVLALQFHAEMGEDERFNAWIEQWPGCIAEAGGDEASLRRAHTEHGPPAVA
                     AGRAMIAEWLAGLDC"
     gene            8558..9244
                     /locus_tag="VN627_10980"
     CDS             8558..9244
                     /locus_tag="VN627_10980"
                     /inference="COORDINATES: protein motif:HMM:NF044451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXD03819.1"
                     /translation="MRHARLMSTLCPAPTASVEPRAEQPRRRQPIRDAQSARTRANLI
                     AAARTLFAANGYHATGVRDITAKAKVTPGALIHHFGDKERLFRAVFDELEHEMLAGAD
                     KAANDLERHTFEGFCQGVKFFIDAAAEPTFQRIVLIEAPAVFGWARWRELEEQFNLGT
                     LRQLLREFMDTGVIRRQNEVYLANLIFASIIEAGLLVATADDPATARSEVSGMMIELL
                     SGMLIGTTAA"
     gene            complement(9342..>10456)
                     /locus_tag="VN627_10985"
     CDS             complement(9342..>10456)
                     /locus_tag="VN627_10985"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD03820.1"
                     /translation="EGRMPARLRDRAPRVVENADGSQSWLWEDRAYPQIAICAVVGRP
                     KDRWGWEATRFEDTRRGCYDPVERIRDMDLDGVQASLNFPSFMPGLGGHNFWMGTQDP
                     ELGLACVRAWNDWFFEDWYSAYPDRIIPCGITWLGDPQIAAAEVRRNAARGFRALSFV
                     GTPSQIGLPDLRGDYWDPLLRACEETDTVICLHVGSDDWNASPPGASVDVGAICFPLS
                     AYRSAADWLWSGALVRFPRLKIAMSEGGLAWVPMLIDRVSYVMEHSAATNVDQWSSRS
                     VHPIEVLKNNFYYCAIEFGSGMDMHERIGIDHIMVESDYPHADSSWPNTQEGLRAALA
                     GFTPEQIRKITWENASKLFRFDVSQAALQLAQPLPA"
BASE COUNT         1782 a         3324 c         3623 g         1727 t
ORIGIN      
        1 gcgttctaca ccaaggcgct gggcatgaag cgggtcctgc gggtcagccg gccgcaggat
       61 ccctatatcg aggatgccta cagtttctcg ggcgatccac tcgccgccga gcccctggtg
      121 atcctggtgc agcacaacga ccgcaagacg gcgccgccgc cttcggcgat cgtgttgggc
      181 gtgcatgtgg ccgatgccca tgccgccgcg gcgaaagtac gggcagccgg ctacgccgtc
      241 ctgcgcgagc cgccgcctga agaccatggc gcaaagctgg cgacgatcgc gaaggatccg
      301 gacggcaaca ccatcgaact ggtccaactg gacatcgccc gcctgcgctg aggcgaattg
      361 agaaatcgag atggcaagcg gcctgcgccg ccaacgcgga aatgaggaga ttgcaagtga
      421 gggatcgact ggccggcaag cggatcatcg tgtttggcag cgccaccggc attggcgcgg
      481 ccacggtcag gcgactggtt gaggaaggcg cccgtgtctg cgccgcagat atcaacctgg
      541 cgggagccac gcaggttgca gccgaggccg gcggcgacac tttcgcggtt tacgtggaca
      601 ttgccgacga ggcttcggtg aacgcggcgg tcgaaacggc ggtgggtcgt ctgggcgggc
      661 tggacggcgc gcacatcaac gcggccgatc tgcgcgtcat catggaggac agcgatgcgc
      721 tcgccttgga cctggccgtg ttcgatcgca cgatcgcggt aaacctgcgc gggcacctgc
      781 tctgcacccg tgccgtgttg ccgcacctcc tgcgcaattc ccagagcgct gtggtctaca
      841 ccagttcggg ctcggctcat ggggccgaac ccgcgcgccc gtgctatgcc atggccaagg
      901 ccggcatcaa cgccttgatg cggcacgttg cggcgcggtg ggggaaggaa gggcttaccg
      961 ctaacgtgct ggcgcccggg ttcaccgtga ccggcgagtt gaaggcgcaa atggaggcca
     1021 acgcggaaga agccgtcaag tggtcgagct actacatgga ccgcacgccg catacccgcc
     1081 tgggggaatc ggaagatcac gccggggtgg tcgccatgct gctgtcggac gacggccgct
     1141 ggatcaacgg tacgatcatt gacgtcaacg gcggctcgct catgcgcggc taagattcac
     1201 tgcgccgggc gaggcgacct gatctgggcg cgatagctgc gcggagtctc tccggtccat
     1261 tcccggaatg cccgtcggaa cgcgtcagaa tccgagaaat cgccgcggcg ggagatttcc
     1321 tcgatcgaaa agtcggtgcg gctgagcaga tcgagcgtga tttcgcggcg gcagctgttg
     1381 cggatttccc gataggaggc tccctcactg gcgagccatc gccgcagggt tgcgctgctg
     1441 caaccgaacc gacccacgat ttcctcgaat gaggggaggc gcgagtgtcg gcgcaattcc
     1501 tcgatggcga tagccctgac cttgggcaag gcagccgttt gggcgggaat gtcattcggg
     1561 tcgagcaggg cgaggctggc cggcgcagag gtctgcccgc gttcccctgt cgtacgcaca
     1621 accggatagt ccaggtagga ggccgatagg gcaaaaccgc tccatcctgc ctccgggtgc
     1681 acggggaagg gcaggcgcgg cagcccgagc gtgcgataga cggctgcggg gtaatcgagc
     1741 gcgaattcga tgatcggtag cgattgcccg atcagccacg aaagttcggc atgcatttgc
     1801 aggatgccgg tgaggtcgat gaagcaattc gttgccgtgc gctgccggcg cagggtttcg
     1861 aaccggagct ccaccaggtc ccctcgaata tgcaagctca tcaagccgca acggccgtcg
     1921 atcgcctcca tgcaatcacc cgcccgttcg atcgactctc gcaaggtgct gccgccctcg
     1981 aggccaaaga gaatgacctt ccagtcgacc gggcggaggg gcaagcggcc ttccgttgcc
     2041 tgggcgttgc gcgacaggtg cgacacggcg cgggtgtaga ggcgctggag gtcggtgtgg
     2101 gaaagcagct cacggccgtc aaggaaggca tgggcattgc agcggatctg caattcggcg
     2161 gcaagatcct gagcgctgcc gacttcccgt tcgtagagcg caacgacgtg ccggaccatt
     2221 gattctgctg tcggccagct ggctggctgg atggaggagg ccattttatc cccttggatt
     2281 cgatcaagga aactaaccat accgccgggg ttgatcaatg tttactgtta tatagacgta
     2341 ttccagctca cctttatcgt gcatttttga tcgaaaatcg ggtgtatgtg gtagcggcgg
     2401 catggcaaat atcgctgacg ggcctggaac gggctgttgg agaggatcat gtcgaaaaag
     2461 agcgccgctg ccctatctgg catggccctt gtgtgtctcg ctgcggttgg actgcatgcg
     2521 gctgacgcgc ccaccggcgg ttggccgggc acccggactt tcgtgctcaa cgggctcttc
     2581 gttggcggcg gcggcgatcc caagggctgc ccggcgcagt cgcccggtaa tttcgacgtg
     2641 taccttgcct cgctcccggc ggaccagcga gaggcgctgt cgccgcccgg caaacggggc
     2701 gatttgcaga agcagatgtg gcagcacttc ggtttccgcc gcatggccct gtggccgggc
     2761 gactttggcg ggcgggccga ggtggccaag ctgccgccgg acgtgaagct gcaccagagg
     2821 ccgagcccgg aacagttgcg cgccattgcc gagcttaatg gcttccccaa ggggcggggc
     2881 agcctggcct accaggacca gacgctggcc tattcgagtt gcaccaaccc ttacgacttc
     2941 cccgagctcg cccgcgggta cgtgccttat cagggcaaga tctcgacggg catgaatctc
     3001 gacggcaagg gcggcgcgca gaacttctcg agccccgacg gcgagaaggg gatcgacaac
     3061 cagctctggc gcgcgatcgg ctgttataag cagagccagg agatgaacga cccagccttg
     3121 caggaaaagg tgatgctttc ggcccgatcg cccacgttga tcgaggtgag cgggatcgat
     3181 gatccgcgca acgatccgga cgttacggtg agggtcctga ccggggccga tccattggtg
     3241 cgcgatggcg ccggcaaacc cctggcgcgt gccagctatt cgctcgatcc cgatccggcg
     3301 ctggtcaacg tgacccatgg gcgcatcgtc aacggggagc tgacgaccga tccgttcgat
     3361 ttgcgtttcg tcttccgcga gcagatcatc gacaacctgc ggctggtgcg cggggccgtg
     3421 gtgcgggcgc gcttcaagcc ggatgggaac cttgatggcg ggatctacgg ctacaccacg
     3481 gtgtcgagct tctatgacat ggtcgaacac atgacccagg acggggcgga caacgtcggc
     3541 ttgtcttgcg tcggggtgcg tcaggcgctg gatcgctatg ccgacggcta ccgcgaccgg
     3601 gccacgggca agttcaccgc gatctcgagc gagatgcgtt tccatgccgt gccggcattc
     3661 gtgaccggga cggttccggg cggtgcgctc ggcggcggct cggcaacggc gagggcgatg
     3721 cgatgagccg cgccgcttcg cgtatcctcc tcgccgccgc cggtgctctg gccggtttcg
     3781 gcatcctctc gtcgagcgcg tcgttcggcg gttcggcggc aacgtccgtg ccgcagccga
     3841 gcgttcgccg gctgaccgag agccagtatc gcgccgctat cgccgaagcg tttggaccgg
     3901 agatcgaagt ggtcggccgg ttcgagcccg acatgcgcgt gggcggcctc gccgccgtgg
     3961 gcgcgagctc ggtgtccatc acgccggccg ggttcgaaca atacgatgcg ataggccgcc
     4021 atgtcgccga gcaggttttc gcgcctgccg cgcggtcgcg gctggttggc tgcgggccgg
     4081 acagcggcga ccccgccgga gcgcgttgcg cggcgggttt cgtcagccgg gtcgggctga
     4141 aactgttccg ccgaccggtc gcgccgcgcg aggtccaggc cgcggtcgcg cggaccctgg
     4201 cctcggcgca gcgcctcggc agcttcgacg ccgggctgca ggcaacgctc gtcggcatgc
     4261 tgaccagccc ggacttcctg ttccgcatcg acctgccggc gccgcacggc ctggcgggca
     4321 cgatcgatgg ctattccaag gcgagccggc tgagcttcct cttgtggaat gcaccgccgg
     4381 acagcgcgct gctggaggcc gcggggaaag gcctgctgga cagcccggac ggcctcggcg
     4441 cccaggttga ccggatgatc gcctcgccga ggttcgtgga tggggtgcgg gccttctttg
     4501 acgatttcct gcaactgcgg gacatggaga cgctgtcgaa ggacgggctg atttatccga
     4561 acttctcgac aaccgtggcc gctgcctcgc gcgagcaggt gctgcgcacg atcaccgacc
     4621 tgctggtcga acacaagggc gactatcgcg acctgttcac cacgcgccgc tttgcgatga
     4681 cgcgtgcgct cggcccgatc tacaacgtgc cggtgagcgg ctcggggtgg tcgatgtacg
     4741 aattccccga aggcgatccg cgcatcggcc tactgaccca ggcgggcctg cttgcgctcc
     4801 attcgcaccc ggggcgcacc tcgcctacgc tgcgcggcaa gtcgctgcgc gagaccgtgt
     4861 tgtgcgagca ggttccttcg cctcccgcca acgtcaattt ctccgtcgtg caggacgtca
     4921 acaacgtcag cctcaagact acgcggcagc ggctggaagc ccacctcgac gatgaggaat
     4981 gcgccagctg ccataaggcg accgatggcc tggggctttc gctcgaacag ttcgatgggg
     5041 ccgggcaatt ccgcagtacc gagcacgacg tgccgatcga cacctcgggc aagttcgaca
     5101 aggcggtgtt cgccggcgtt ccggcgctgg cccggctgat gcacgacagc cccaaggtga
     5161 gccagtgcgt ggtgagtacg gcctggcgat acgccaacgg gcgcgacgcg gggccggcgg
     5221 atcgcccgga gctggatcgc ctgcacgcca gcttcgcggc gagcggctat cgcttcatcg
     5281 acctgatgcg aaccatcgcg cttgattcgc aattctacgc gatgccccgc attccccagg
     5341 cccggccggc agcgaccgcc cgcgccgaca gcaaatcgca ggaccgctca tgaccagtcc
     5401 gctctctcgc cgctccttcc tcatgcgtgg cgcgctcggc ggcgcggccg tggtcgtcgg
     5461 cgtgcccttg ctcgacctgt tccttgacga cagcgggacg gccttcgcgg cttcggtggg
     5521 gggtggccgg ctcccggttc ggtttgggac gtggttctgg ggctgcggca tgatcccgga
     5581 gcgctgggtg ccgaaagcag cgggaaccgg gtatgacctg ccgccgcagc tggccccgat
     5641 caagccggtg caggagcatg tctccgtgct gtcgggctat gacgtgatgc tcgacggcaa
     5701 ggcgaacctc ccgcacattt cgggcaacac cgcggttcgc accggcgagg cctcggacac
     5761 ctggcagggg atcagcgggc cgaccttcga cgtcctgatc tcggacgcga tcggttcggg
     5821 ggcctacttc cgcgcgctgg acctcagcct ggatggcaat ccgcagacca gctattcctt
     5881 ccgcaacggc gcgagcatga atgccgccat tccgagcccg gtcgagcttt accagaaggt
     5941 gttcggcctc gattttcacg atcccaacac cgccgacttc aagcccgatc cgcgctacat
     6001 ggtgcgccgg agcgtgctct cgggcgtgac cgagcagcgg cgggcgctgc tgtcgcgcgt
     6061 tggggcggcg gaccgggcgc ggctggacca gtacttcacc tcggtgcgcg agatggagga
     6121 caagctggcg ctgcagctgc agaagccgcc gccggccgaa gcctgcgttc gtccggacgc
     6181 gccggcagcg gtgcgcggcg acatcaccga ccttggcgtg cgcaaggcca atcacaagct
     6241 gatgacccag ctgctgggca tggccctggc ctgcaaccag acgcgggcgt tcaacatgaa
     6301 cttcgacacc gcggcagccg agctgcggcg ggcgggcgat accacgggct accaccagag
     6361 cacccatgag gagctggtcg accgcagcgt gggctaccag ccgacggtcg acaagttcgc
     6421 gaccgggagc atggaggcct gggcggactt cgtctcgatc ctttcgggca ttcgcgaggg
     6481 agacgggagc ctgctggata acctgctggt cgtcgctcat tccgaggtgt cgtttgccaa
     6541 gaaccacgac gtcaccggga tcccggtgat gcttgcgggc cgggcggggg gcaaggtcaa
     6601 gcagggccag cacatcaagg gcgccggcga gccgatcacg cgggtggggc tgacggcgca
     6661 gcaggtgatg ggggtgccgg tggacagctg gggccgcggg gcgatgcgga gcaaccgggc
     6721 gatcggcgag attctcgcgt gaggcgaatt gcaagggaag gtatgttgat gaagcacatg
     6781 cgttgggctg cggccgggct ggtcctggtg gcgggggcgg gggctgcggt ggcggcggcg
     6841 ccggcggtga tcgccgcgat ccacgcgcgg caggccagtt tcaaggaaat gggcggggat
     6901 ttcaaagcga taagcgatga gatcaagtcc ggctcgccgg actggccggc ggtgcggcga
     6961 tcggcgcatg acctgtcggc cagggctccg ggccaggaac ggctgttcgt gcgcggcagc
     7021 ggtgcggaag cgggcgtgcg cacccgcgcg ctgccgctga tctgggccga cccggcgggc
     7081 ttcgcgcggg ccaacgctga cctcgtgcgc gccgccgccg tgctggacgc agcggcccag
     7141 cgcggggacc ttgccgggct catcaatgcg cgcgcgatgg cgggcgcgac ctgcaagggg
     7201 tgccacgaca agtaccgcgc aaccgacgat tgaataaggg gcggggcgat ccatccggca
     7261 cggccggaga ggtgacgtcc ccgctcagca gtcgaggccg gccagccatt ccgcgatcat
     7321 cgcacggccc gcggcaacgg ctggcgggcc gtgttcggtg tgtgcccggc gcaggctggc
     7381 ctcgtcgcca ccggcttcgg cgatgcagcc gggccactgc tcgatccatg cattgaagcg
     7441 ttcgtcttca cccatctcgg cgtggaactg aagggcaagg acattgcgcc cgtagcggaa
     7501 ggcctgatgg tcatagaggt ggctcgaggc cagcagctga gcgccgcccg gcaaggtgaa
     7561 cgtatccccg tgccaatgca gcacgggcac ttcccgcaag tggcgcatga aggcggcatc
     7621 gtgctgtggc gcgatgctga ccgggtggaa gccgacttcc ttgaccggcc cgggatagac
     7681 ccgggcgcca agcgccgcgg cgatcatctg ggcgccgaaa catacgccga gcgttggccg
     7741 gtcagcctcg aggcgcggtg ccaggcggcg cagctggcac gcgatccacg ggtgctgatc
     7801 ctgctcgtag acgcccatcg ggccacccat catgatgagc aggtcgggct cggcaaggtc
     7861 aaccgatgcg aaggccggat cggcgacatc gatccggtcg acatcgtagc ccgcagcctc
     7921 gatcggctgg cgaaaaccgg cgaccccttc gtagggaaca tgccggatga tcagtgcctt
     7981 cttcatggtg ctctctcggc gtgctcagcc cccggcatcc gggcgcagag gctgtctact
     8041 tggccagccg agtagcgcaa aaagggaaaa ttttgccgag accatagccg cgctttgaag
     8101 cgtgcgcatc cgccgcttca ggcgccggag atgtcgcacg tgaaaccgac ctgtggcaca
     8161 agcgaggccg gccctcgctc gtgaccggat caggctgggg aggctgcggg cgccatcctc
     8221 atgagcatgg tccggtaata cctggccagg gcgagatggc gggcccgcct ttcggccgcc
     8281 cgcgcccagc tggcaacgag atagcagcga tcaatgcggc gcgtgaggaa atcgcgagtc
     8341 cacaagccct gcatcgttcg tcccttccat tccaaagtga gtggcgcagg atagtcccgg
     8401 tggcccaacc ataaagcgtt cagagccgaa atgcggcatg gctgcgccaa atgaccattt
     8461 tttccagcga gccgggaacg atccgcaagc cttctgttgg ccccgatcga gaaatgacat
     8521 agaaccggca agcggccttc ggcttgcttt aaccagaatg cgtcatgccc gtctgatgtc
     8581 aaccttgtgt ccagcaccga ccgcctctgt cgaaccgagg gcagagcagc cgcgacggcg
     8641 gcagccgatc agagatgcgc agtccgccag gacgcgcgcc aacctgatcg cggctgcgcg
     8701 gaccctgttc gcggccaatg gctatcacgc cacgggggtg cgagacatca ccgccaaggc
     8761 gaaggttacc cccggcgcgc tgatccacca cttcggggat aaggagcggc tgttccgggc
     8821 cgtgttcgac gagctcgaac acgagatgct ggcaggcgcg gacaaggccg ccaatgacct
     8881 tgagcgccac acgttcgaag gcttctgcca aggcgtcaaa ttcttcatcg acgcagcggc
     8941 agagccgaca ttccagcgca tcgtcctgat cgaggcgcca gcggttttcg gctgggcgcg
     9001 gtggcgggaa cttgaggagc agttcaacct gggcaccctg cgccagttgc tgcgcgaatt
     9061 catggatacc ggggtcatca ggcgccagaa tgaggtttac ctggccaatc tcatcttcgc
     9121 ttcgatcatc gaagccggcc tgctggtggc tactgccgat gatccggcga ccgcccgttc
     9181 cgaggtgtcc ggcatgatga tcgaattgct ctccggcatg ctgatcggca cgaccgccgc
     9241 ctgaccgtct cgggactggc cagcgagcgg ccgggacgac cctgggcagg ggcgccgacg
     9301 tgatcggcag cgaggggcca gccgatcacg ttgcgcggcg atcaggcggg cagcggctgc
     9361 gccagttgca gggccgcctg cgacacgtcg aaccggaaca gcttcgaggc gttttcccag
     9421 gtgatcttgc ggatctgctc cggtgtaaag ccggcaaggg ccgcgcgcag gccctcctgg
     9481 gtgttgggcc aggaggagtc ggcatggggg tagtcgcttt cgaccatgat gtggtcgatc
     9541 ccgatccgtt cgtgcatgtc catgcctgag ccgaactcga tggcacagta atagaaattg
     9601 ttcttcagca cctcgatcgg gtggaccgag cggctgctcc actggtcgac attggtcgcc
     9661 gccgagtgct ccatcacgta gctcacccgg tctatcagca tcggcaccca ggcaaggccc
     9721 ccttccgaca tggcgatctt gagacggggg aagcgcacca gcgcgcccga ccacaaccag
     9781 tcagcggccg aacggtaggc cgacagcggg aagcagatcg cccccacatc gaccgatgcg
     9841 cccggggggc tggcgttcca gtcgtcggag ccgacgtgca ggcagatcac ggtgtcggtt
     9901 tcctcgcagg cgcgcaggag cggatcccaa tagtcgccgc gcaggtccgg caggccgatc
     9961 tgggacggtg tgcccacgaa gctgagcgcg cggaagccgc gcgcggcatt gcggcgcacc
    10021 tcggcggcag cgatctgcgg atcgcccagc caggtgatcc cgcaagggat gatgcgatcg
    10081 ggataggcgc tgtaccaatc ctcgaagaac cagtcgttcc aggccctgac gcaggccaga
    10141 cccagttcgg gatcctgtgt gcccatccag aaattgtggc cgcccaggcc cggcatgaag
    10201 gacgggaaat tgagcgaggc ctggacgccg tcgaggtcca tgtcgcggat ccgttcgacc
    10261 gggtcgtagc agccacgccg ggtatcctcg aagcgcgtcg cctcccagcc ccagcggtcc
    10321 ttcggccgac cgacaaccgc gcagatggcg atctggggat aggcgcgatc ttcccacagc
    10381 cacgactgcg agccatcggc gttctcaacg acgcgcggcg cgcggtcacg caacctggcg
    10441 ggcatgcgcc cctcga
//
LOCUS       DATNBV010000463         2966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4096664,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000463 DATNBV010000000
VERSION     DATNBV010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4096664"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VN627_10990"
     CDS             <1..856
                     /locus_tag="VN627_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HXD03821.1"
                     /translation="QMSVNPAKPGSEVHGYLDLKTLPRDKSGRFDVVLSPARPAGWRG
                     SWYQLDPQASTLVVRFVASDWEHERDPTLAIERLDAPATRPRVPAAVLEQRLRRLPDA
                     VDATALMFIDHVSKLFAEGYVNRLKVFDVSQIGGQLTGQFYYEGGYDLADDEALVVEA
                     KVPAKCLYYSMILTNPIYETTDWVNNQSSLNDSQLHVDPDGVLRVVISARDPGVANWL
                     DTAGYAKGVVQGRWTECSAQPVPSARKVKLSELATVLPAATPRVTSAERERILRDRRA
                     AFQQRTLW"
     gene            complement(876..1646)
                     /locus_tag="VN627_10995"
     CDS             complement(876..1646)
                     /locus_tag="VN627_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HXD03822.1"
                     /translation="MAHWTPAPRFREFRISTGYPARADRLRRFAILLAALAAGLLLPG
                     APATAQVAANITLATNQLFRGETISGDDPGLTVAVSADLPGGLFAGADVSLAAGGESP
                     RFTASNQYAGIAKRLGGASLEAGAIHRIYAPLFDEAYRRHYTELYAGISLHKVQLRAF
                     VSPDYLADGRTTYYIEANARLATIGQWKLEGHGGISLIPHDLDSPEHGLISYEDWSLW
                     ASRGVGPFTFGVGIAATNYPVFGPSGKVRLSAQLSRSF"
     gene            complement(1576..2835)
                     /locus_tag="VN627_11000"
     CDS             complement(1576..2835)
                     /locus_tag="VN627_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXD03823.1"
                     /translation="MIGRSLLALLLAVMAAAVALGPRPARAAPAAQAPAAAEAQAARR
                     VMMMLDLGPEHYRSGSDYGGDYGDAMGAKARLRFARKVAREHRLAVVDSWPMPLIGVD
                     CIILEIRDGRTIDAVVRELSAVPGVSWSQPLNQFEMLEARPPSPARAPAVSYNDRLFS
                     AQPAASLWHLARLHAVATGRGVTVAVIDSRIDVGHPDLAGQISGAVDFVPGRPQAERH
                     GTGVAGIIASRSNNSQGIAGVAPGARIMALRACWERTGGGATVCDSLTLARALTYAIE
                     HGAGVVNLSLTGPHDQLISRLVAMAMARGAVVVAAIDEQNPGASFPAFLPGVVSVGDE
                     RLSDRLQTAYKAPGQDVLTTQPEGKWDLVSGSSYAAAHVSGLMALLRQLSGTRTRPDA
                     LLGPHGTLDACAAVSRVSHLDRISCAR"
BASE COUNT          470 a         1027 c         1041 g          428 t
ORIGIN      
        1 gcagatgtcg gtcaatccgg ccaagcccgg cagcgaggtg cacggctacc tcgatctcaa
       61 gaccctgccg cgcgacaagt ccggccggtt cgacgtggtg ctgagcccgg cccgcccggc
      121 cgggtggcgc gggagctggt accagctgga cccgcaggcc tcgacgctag tcgtgcgctt
      181 cgtcgcctcc gactgggagc acgagcgcga tccgaccctt gcgatcgagc ggctcgatgc
      241 gccggcgacc cgtccgcgcg ttcccgccgc cgtgctcgag cagcgcctgc gccgcctgcc
      301 cgatgcggtc gatgccacgg cactgatgtt tatcgatcac gtgagcaagc tgttcgccga
      361 gggctacgta aaccggctca aggtcttcga cgtctcgcag atcggcggcc agctgacggg
      421 ccagttctat tacgagggtg gctatgacct ggccgatgac gaggcgctcg tggtcgaggc
      481 caaggtcccg gcgaagtgcc tctattactc gatgatcctg accaatccga tctacgagac
      541 gaccgattgg gtcaacaacc agtccagcct caacgacagc cagctgcacg tcgatcccga
      601 tggcgtgctg cgcgtggtga tctcggcgcg tgatccgggc gtcgccaact ggctggatac
      661 ggccggctat gccaagggcg tggtccaggg ccgctggacg gaatgcagcg cccagccggt
      721 gccctcggcc cgcaaggtca agctgtccga actcgccact gtcctgcccg cggccacccc
      781 gcgcgtgacg tcggccgaac gcgaacgcat cctgcgcgac cggcgcgccg cgttccagca
      841 gcgaaccctg tggtaaaccg gccggtggcc gatcgtcaga acgaccggct cagctgggca
      901 ctgaggcgaa ccttgccgct cggcccgaac accgggtagt tggtggcggc gatccccacc
      961 ccgaaagtaa acggaccaac gccgcggctg gcccagaggc tccagtcctc gtagctgatc
     1021 aggccatgtt ccgggctgtc gaggtcgtgg gggatcagag atatcccgcc gtgcccctcc
     1081 agcttccatt ggccgatcgt cgccaaccgt gcgttcgcct cgatgtagta ggtcgtgcgc
     1141 ccgtccgcga ggtagtcggg cgagacgaat gcccgcagct gcaccttgtg caggctgatc
     1201 ccggcataga gctcggtata gtggcggcgg taggcctcgt cgaacagcgg ggcatagatc
     1261 cggtgaatcg ccccggcttc gagcgaggca ccgcccagcc gcttggcgat cccggcatat
     1321 tggttcgagg cggtgaagcg cgggctctcg ccgccagcgg caaggctcac gtcggctccg
     1381 gcaaacagac caccggggag gtcggcactg acggccaccg tcaggcccgg atcgtcgccg
     1441 ctgatggtct cgccgcgaaa cagctggttg gtggcaaggg tgatattggc cgccacctgc
     1501 gccgtggccg gcgcgccggg caggagcagt ccggcggcca gcgccgcaag caggatggca
     1561 aaccggcgca ggcggtcagc gcgcgcagga tatccggtcg agatgcgaaa ctcgcgaaac
     1621 cgcggcgcag gcgtccagtg tgccatgggg gccgagcagc gcgtcaggcc gggtacgcgt
     1681 gccggagagc tgccggagca gggccatcag cccgctgacg tgggccgcgg cataggaact
     1741 gccgctgacg agatcccatt ttccctccgg ctgcgtggtc aggacatcct gtccgggggc
     1801 cttgtaggcc gtctgcaaac gatctgacaa gcgttcgtcg ccgaccgaga ccacgcccgg
     1861 caggaaggcg ggaaagctgg cgccggggtt ctgctcgtcg atcgcggcga cgaccaccgc
     1921 accgcgcgcc atggccatcg ccaccagccg gctgatcagc tggtcgtgcg gcccggtcag
     1981 gctgaggttg accacgcccg cgccgtgctc gatcgcatag gtcagcgcgc gggcaagggt
     2041 caggctgtcg cagaccgtgg cgccgccgcc ggtccgctcc cagcaggccc gcaatgccat
     2101 gatccgcgcg cccggggcca ctccggcaat gccctgcgaa ttgttcgacc gtgaggcgat
     2161 gatcccggca acgcccgtgc cgtgccgctc ggcctggggg cggcccggca cgaagtcgac
     2221 cgcgccggag atctgtccgg caaggtcggg atggcccacg tcgatccggc tgtcgatcac
     2281 cgcgaccgtc acgccgcgcc cggtggcgac ggcgtggagg cgcgccaggt gccaaagcga
     2341 agccgccggc tgtgcggaaa acagccggtc gttatagctg acagcggggg cccgggcggg
     2401 cgagggtggt cgcgcctcaa gcatctcgaa ctggttgagc ggctgcgacc atgagacgcc
     2461 gggcacggcc gaaagttcgc gcaccacggc atcgatcgtt cgcccgtcgc ggatttccag
     2521 aatgatgcag tccaccccga tcagcggcat gggccacgaa tcaacgacgg cgagccggtg
     2581 ttcgcgggcg accttgcggg caaagcgcag gcgggccttg gcccccatgg catcgccata
     2641 gtcgccgcca tagtcgctgc ccgaacgata gtgctccggc ccgaggtcga gcatcatcat
     2701 cacccggcgg gcggcctgcg cctctgcggc ggccggggcc tgggccgcgg gtgcggcgcg
     2761 ggccgggcgg gggcccagcg ccacggccgc cgccatgacc gccagcagca gggccagcag
     2821 gcttcgcccg atcatggccg gcctccgtcg agcggttcgg ccagcaccac gccgctggtt
     2881 gcgcacagct tggcgaggac ggcatcgcgc cgctccggat cgatcgccag ccgggcggtg
     2941 ccggcagccg aaggtgcccc caccat
//
LOCUS       DATNBV010000464         2387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3466550,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000464 DATNBV010000000
VERSION     DATNBV010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3466550"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VN627_11005"
     CDS             <1..452
                     /locus_tag="VN627_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03824.1"
                     /translation="AMSVDWPIQKKHEHIAVSADNAWMLLRNEPMVILRRFSPKEDDR
                     RVTAAPYFGNLRGAFIGIENHLNYVSRPGGKMSVSETLGLAAFLNSRLVSDHFESISG
                     HTQVNATDLRALPLPSVAQLEGMGELVASARSVTAADQAVAEVLRVR"
     gene            complement(445..2118)
                     /locus_tag="VN627_11010"
     CDS             complement(445..2118)
                     /locus_tag="VN627_11010"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HXD03825.1"
                     /translation="MNSDFDYIIVGGGSAGCVLAGRLSEDPQVRVALLEAGPPDTSVL
                     IHCPAGLALIAKTGQANWALATVPQPGLNGRRGYQPCGKVLGGSSSVNAMVYIRGHRN
                     DYDHWAEEGNTGWSWNDVLPYFKRAEHNERGADEFHGVNGPLNVMDLRSPNQFGPVFV
                     QAAVQAGYSHNRDFNGEQQEGFGIYQVTHRNGERFSAAKAYIAPNLGRPNLQVITDAH
                     TTRVLMEGRQAIGVEYRQGGELKQLRAAREVLLSAGALKSPHILMLSGIGPAAQLQAH
                     GIAVTHDLPGVGRNLHDHPDVIQVVDAPNLTDLFGLSHRTIGSIVKAIFEWRGSRSGR
                     LTTNFAEAGGFVRSGPDEPVPDLQLHFVIAKLVDHGRKTVLGLGYSCHVCLLRPDSRG
                     SITLASSDPMAAPLIDPNFLGERSDIERMVRGFKITRNILGQSALASYGGRELPASAA
                     ARTDEEIEAFIRGHADTIYHPVGTCRMGSGGLDVVDAQLCVRGVERLRVVDASIMPRI
                     VAGNTNAPTIMIAEKAADMIKAAARGQERGRTASRLDSASEFKEPMGII"
BASE COUNT          393 a          789 c          768 g          437 t
ORIGIN      
        1 aggccatgag cgtcgattgg ccgattcaaa agaaacacga gcatattgcc gtgagcgcgg
       61 ataacgcatg gatgctgctt cgcaacgagc cgatggtgat cttgcgaaga ttcagtccga
      121 aagaggacga tcggcgggtt acggcggccc cttatttcgg gaatttgcgg ggtgccttca
      181 ttggtatcga gaaccatctc aattacgtca gccggccggg cggcaagatg tccgtcagcg
      241 aaacgctggg gctggcggca ttcctgaata gccgcctggt gagcgaccat ttcgagtcca
      301 tcagtggcca cacgcaggtg aacgcgaccg acttgcgggc gctgcctttg ccatcggtgg
      361 cgcagctgga aggtatgggg gaactcgtgg cctccgcccg gtcggtgacg gccgccgatc
      421 aggcggtagc cgaagtcctc cgcgttagat aattcccatc ggttccttga attcacttgc
      481 ggagtcgagc cgcgatgcgg ttcggccgcg ctcctgcccg cgcgccgctg ccttgatcat
      541 gtcggcggcc ttctcggcga tcatgatggt cggggcgttg gtgttgcccg cgacgatgcg
      601 cggcatgatg gacgcatcca ccacgcgcag ccgttccacg ccacgcacgc acagctgcgc
      661 atccaccacg tcgagcccgc cgctgcccat gcggcaagtg cccaccgggt gatagatggt
      721 gtcggcatgg ccgcggatga aagcctcgat ctcctcgtcg gtgcgcgccg cggcggaagc
      781 cggcagctcc cggccgccat aactcgccag cgcgctctgg ccgaggatgt tgcgcgtgat
      841 cttgaatccc cgcaccatgc gctcgatgtc gctgcgctca cccaggaagt tcgggtcgat
      901 cagcggagcc gccatcggat cactactggc cagggtgatg ctgccgcggc tgtcgggccg
      961 caacaggcac acatggcacg aatagccgag accgagcacc gtcttgcgcc catgatcgac
     1021 cagtttggcg atgacgaaat gcagttgcag gtccggcacg ggctcgtccg ggccgctcct
     1081 gacgaaaccg cccgcttcgg cgaagttggt ggtgagcctg ccgctgcggg aaccgcgcca
     1141 ctcgaagatg gccttcacga tcgagccgat ggtgcggtgc gacaggccga acaggtccgt
     1201 gagattcggc gcatccacca cctggatcac gtccgggtga tcgtgcaggt tgcgccccac
     1261 gcccggcaag tcgtgtgtca cggcgatgcc gtgggcctgc agctgcgccg cgggcccgat
     1321 gcccgaaagc atgaggatgt gcggggactt gagggcgccc gcgctgagca gcacctcgcg
     1381 cgcggccctc agctgtttga gctccccgcc ctggcggtat tcgactccga tcgcctgccg
     1441 gccttccatg agaacgcgcg tggtgtgggc gtcggtgatc acctgcaggt tgggtcgccc
     1501 cagattgggc gcgatataag ctttcgccgc gctgaaacgc tcgccgttgc ggtgcgtcac
     1561 ctgatagatc ccgaagcctt cctgctgctc gccgttgaaa tcgcggttgt gcgagtaacc
     1621 ggcctggacg gccgcttgca cgaacaccgg cccgaactgg ttggggctgc gcaagtccat
     1681 gacgttcagc ggtccgttca cgccgtggaa ctcgtccgcg ccgcgctcgt tgtgttcggc
     1741 gcgcttgaag tacggcagca catcgttcca gctccagccg gtatttccct cttcggccca
     1801 atggtcgtag tcgttccggt ggccgcggat gtagaccatc gcattgaccg aactcgatcc
     1861 gcccagcact ttgccgcacg gctggtaccc gcgccgcccg ttcagtcccg gctgcggcac
     1921 ggtggccagc gcccagttcg cctgtccggt cttggcgatc agcgccaatc cggcgggaca
     1981 gtggatcagc acactggtgt cgggcgggcc ggcctccagc agcgcgaccc tgacctgagg
     2041 gtcttcgctc aggcggcccg ccagcacaca gccggccgat ccgccgccaa cgatgatgta
     2101 gtcgaaatca gagttcatac aggtgtcatc cggttgccgc tgcaccatac aaccaaaata
     2161 gcatcgggct gtcgactccc tgctctaatg acgcattgcc gggttggctc gaaaaacggc
     2221 ccggccgatt cacggcgcgg tgaatccgca gcgcccgccg cgccgtatag ccgttatggc
     2281 cagcccgttc gcccagcccg tgccctccga cctgcccggc gctacccgca tcgtgaatac
     2341 ggccgagatt gcctgcccgc cggagcggct gttcgagttc gtcacca
//
LOCUS       DATNBV010000465         5158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4383541,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000465 DATNBV010000000
VERSION     DATNBV010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4383541"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VN627_11015"
     CDS             <1..652
                     /locus_tag="VN627_11015"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXD03826.1"
                     /translation="DTFYFPDEGPGGTRMLLRTHTSPVQIRTMLKEGAPLRIIAPGRV
                     YRSDSDATHTPMFHQVEGLVIDKGIHLGHLKWTLETFLKAFFEREDIVLRLRPSYFPF
                     TEPSVEVDVGFAVVSGPDGKSRRVLGGSGDAPGHGWMELLGSGMVNRKVIEFGGLDPD
                     EWQGFAFGIGVDRLAMLKYGMDDLRAFFDGDQRWLSHYGFSALDVPTLSAGVGTPA"
     gene            666..3050
                     /gene="pheT"
                     /locus_tag="VN627_11020"
     CDS             666..3050
                     /gene="pheT"
                     /locus_tag="VN627_11020"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXD03827.1"
                     /translation="MKFSLSWLKDYLDTEASVAEISATLNAIGIEVEGIEDPAARLAG
                     FRVARVLTAAPHPNADKLQVLTVDTGAGDPLQVVCGAPNARAGLVGVLGTAGAVVPAN
                     GMVLKVAAVRGVESNGMMCSSRELELGEDHAGIIELPADAPVGAAFADYHGADPVFDV
                     SITPNRPDCMGVYGIARDLAAAGIGALKPISVLAVDGSFTCPTQIRTDDPAGCPAFHG
                     RVIRGVTNGPSPKWLQDRLKSAGQRPISALVDLTNYLMLGFGRPAHAYDLAKLTGAVV
                     ARKARDGETVLALNGKEYTLDAGMTVIADDAGVHDVAGIMGGEHSGCTEATTDVLLEV
                     AYFDPEAIAATGRKLNLTSDARSRFERGVDPLFLDTGLEMLTRLMIETCGGEASAVIR
                     AGQPPRDPAVVAYDPALAETLGGVGIPAPEQRRILGALGFTVSDDWTVTAPGWRPDIA
                     GAPDIVEEVVRIWGLDNIASVALPRADGVARPTATPAQKLERRLRRAAAARGLNEAVT
                     WSFLPTAEAELFGTDLWVLENPISEDMKAMRPSLLPGLLAAAARNLARGANSLRLFEI
                     GRRYLRGADGASDERLSLAVVLAGDKVARGWATGRAAAFDAFDAKAEALALLAEAGAP
                     IDNLQVMGEAGGQFHPGQSATLRLGPKTVLARFGMVHPATAKAFDLDGPVAMVELFLD
                     AIPGKRGAATFARAAYAPPALQAVTRDFAFLIAADKPAGELLRAVRGADKANIVSARL
                     FDDFRGAGVPEGQKSLAVEITLQPLEKSYAEADLKAIADKVTAAAAKLGAQLRG"
     gene            3106..4632
                     /locus_tag="VN627_11025"
     CDS             3106..4632
                     /locus_tag="VN627_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HXD03828.1"
                     /translation="MTAHNSRRTFAIISHPDAGKTTLTEKLLLQGGAIHLAGEVKARG
                     QARRARSDWMKIEQQRGISVTSSVMTFHKDGITFNLLDTPGHEDFSEDTYRTLTAVDS
                     AIMVIDAAKGIEPQTRKLFEVCRMRSVPIITFINKVDREGRSPFETLDEVADALALDV
                     VPMSWPVGMGGTFQGVLDFATGMVSRPEGDSREFLGKVEEMALPQDVAEEVELARGGY
                     PEFSLDAYRHGDMTPAYFGSALKNFGVAELIDAIAKFAPPPRPQSSDQGTISPDRADV
                     TGFIFKVQANMDPQHRDRIAFMRMVSGTFRRGMKLTPSGLGKPIAVHSPILFFAQDRE
                     IADTAEAGDIIGIPNHGTLRVGDTLSEKNDVRFTGLPNFAPEILRRVALKDPTKTKQL
                     RKALDDLSEEGVIQVFYPEIGAQWIVGVVGQLQLDVLISRLEAEYKVEAVLEPSPFDT
                     ARWLKGTDVALKSFAEFNKSNLAKDRDGDPVFMARSAWDVSYQQEKNPELGFSATKER
                     "
     gene            4685..>5158
                     /locus_tag="VN627_11030"
     CDS             4685..>5158
                     /locus_tag="VN627_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXD03829.1"
                     /translation="MELTVASYNIHKAVGLDRRRDPERIMAVLREIDADVIALQEADR
                     RFGDRVSVLPRQVLEDHGHWQAVALGQRPDSIGWHGNAVLVRRGIEVVDADIVPLPTL
                     EPRGAVRVDLVKHGQPVRVVGMHLDLSGLRRRQQVRTVLAHVEICGEACPTVLMGD"
BASE COUNT          844 a         1687 c         1781 g          846 t
ORIGIN      
        1 cgataccttc tacttcccgg acgagggccc gggtggcacc cggatgctcc tgcgcaccca
       61 caccagcccg gtgcagatcc gcaccatgct gaaggagggc gcaccattgc ggatcatcgc
      121 gcccggccgc gtgtatcgct cggattccga cgccacccac acgccgatgt tccaccaggt
      181 cgaaggcctg gtgatcgaca agggcattca cctcggccat ctcaagtgga cgctggaaac
      241 cttcctcaag gccttcttcg agcgcgagga cattgtcctg cgcctgcgcc cgtcctactt
      301 ccccttcacc gaaccctcgg tggaagtgga cgtgggcttc gctgtggtca gcgggcccga
      361 cggcaagagt cggcgggtcc ttggcggttc gggcgacgcg ccgggtcatg gctggatgga
      421 gctgctcggc agcggcatgg tcaaccgcaa ggtcattgaa ttcggcggcc tggatcccga
      481 cgaatggcag ggcttcgcct tcggcatcgg ggtcgatcgc ctcgccatgc tgaaatacgg
      541 catggacgac ctgcgcgcct tcttcgacgg cgaccagcgc tggctgtcgc attacggttt
      601 ctccgcgctc gacgtgccga ccctgtcggc cggcgtcggc acccctgctt gaggagcgcg
      661 cacccatgaa attctcgctc tcctggctca aggactacct cgacaccgag gccagcgttg
      721 ccgaaatctc ggccacgctc aatgccatcg gcatcgaggt cgaaggcatc gaggacccgg
      781 cggcccgcct cgccggcttc cgcgtcgccc gcgtgctcac cgccgccccg caccccaatg
      841 ccgacaagct gcaggtgctc accgtcgaca ccggcgcggg cgatccgctg caggttgtct
      901 gcggcgcgcc caatgcccgg gcaggcctcg tcggcgtgct cggcacggcc ggcgcggtgg
      961 tgccggctaa cggcatggtg ctcaaggttg ccgccgtgcg cggggtcgag agcaatggca
     1021 tgatgtgttc cagccgcgag ctggagctgg gcgaggacca cgccggaatc atcgagctgc
     1081 cggccgatgc gccggtcggc gcggcattcg ccgactatca cggcgccgat ccggtgttcg
     1141 acgtttcgat cacccccaac cgccccgatt gcatgggggt ctacggcatt gcccgcgatc
     1201 tcgccgccgc cgggatcggc gccttgaagc cgatctccgt cctggcggtt gacggcagct
     1261 ttacttgtcc gacccagatc aggaccgatg atcctgcggg ctgcccggct ttccatggcc
     1321 gcgtgatcag gggcgtgacc aacggccctt cgcccaagtg gctgcaggac cggctgaaga
     1381 gcgcaggcca gcgcccgatt tcggccttgg tcgatctcac caattacttg atgctcggct
     1441 tcggccgccc cgcccatgcc tacgacctcg ccaaactgac cggcgctgtc gtcgcccgca
     1501 aggcgcgcga cggggagacg gtgctggcgc ttaacggcaa ggaatacacc ctcgacgccg
     1561 ggatgaccgt gatcgccgac gatgccggcg tccacgatgt tgccgggatc atgggcggcg
     1621 agcattcggg ctgcaccgag gcgacgacgg acgtcttgct ggaagtggcc tacttcgatc
     1681 cggaagccat cgccgccacg ggccgcaagc tcaacctcac ctcggacgcg cgcagccggt
     1741 tcgagcgcgg ggtcgatccg ctattccttg acaccggtct ggaaatgctg acccggctga
     1801 tgatcgagac ttgcggcggc gaagccagtg cggtgatccg tgccggtcag ccgccgcgcg
     1861 atccagccgt ggtggcctat gacccggccc tggcggaaac gttgggcggc gtcggcatcc
     1921 ccgcgcccga gcagcgccgg atcctcggcg cgctgggctt caccgtgtcg gacgactgga
     1981 cggtgacggc gcccggttgg cggcctgata tcgccggggc gccggacatc gtggaagaag
     2041 tggtccgcat ctgggggctg gacaacattg cgagcgtggc cctgccgcgc gccgatggcg
     2101 tggccaggcc gacagccact ccggcgcaga agctggagcg gcggctgcgg cgggcggcag
     2161 cggcgcgggg cctcaatgaa gcggtaacct ggtcgttcct gcccacggcg gaggccgagc
     2221 tgttcggcac ggacctgtgg gtgctggaaa acccgatcag cgaggacatg aaggccatgc
     2281 ggccctcgtt gctgccgggc ctgcttgcgg cggcggcgcg caacctggcg cgcggcgcca
     2341 actcgctgcg cctgttcgag atcgggcgcc gctacctgcg cggggctgac ggcgccagcg
     2401 atgaacggct gtccttggcc gtggtgctgg cgggggacaa ggtcgcccgc ggctgggcca
     2461 cgggcagggc cgcggcattc gacgccttcg atgccaaggc cgaggcgctg gccctgctgg
     2521 ccgaggcggg cgccccgatc gacaatctgc aggtgatggg cgaggccggc gggcagttcc
     2581 acccgggtca gtcggccacg ctgcggctgg ggcccaagac tgttctggcc cgcttcggga
     2641 tggtgcatcc ggcgacggcc aaggcgttcg atctcgacgg gccggtggcg atggtcgagc
     2701 tgttcctcga tgccatcccg ggcaagcgcg gggcggcgac cttcgccagg gccgcctatg
     2761 ctccgccggc gctgcaggcg gtgacgcggg actttgcctt cctgattgcg gcggacaagc
     2821 cggcgggcga gcttctccgc gcggttcgcg gggcggacaa ggcgaatatt gtcagcgccc
     2881 ggctattcga cgacttccgc ggtgcaggcg tgccggaggg ccagaagtcg ctggccgtcg
     2941 agattacgct ccagccgctc gaaaagagct atgccgaggc ggatctcaag gcgattgccg
     3001 acaaggtcac cgcggcggca gcgaagctgg gggcgcaact gcgcgggtga ccctcgcgcg
     3061 agacagtgga cctgcgggcg atccccggct aggcgccggg gcgccatgac cgctcacaat
     3121 tcccgccgca cctttgccat catctcgcat cccgacgcgg gcaagaccac gctgaccgag
     3181 aagctcctgc tgcagggcgg ggcgatccat ctcgccggcg aggtcaaggc gcgcggccag
     3241 gcgcggcgcg cgcggtcgga ctggatgaag atcgagcagc agcgtggcat ctcggtgacc
     3301 agctccgtga tgaccttcca caaggatggt atcacgttca atctgctcga taccccgggc
     3361 cacgaagact tctcggaaga cacctaccgg acgcttactg ccgtcgattc cgcgatcatg
     3421 gtgatcgacg cggccaaggg catcgagccg cagacgcgca agctgttcga ggtttgccgg
     3481 atgcggtcgg tgccgatcat caccttcatc aacaaggtgg accgggaagg ccgctcaccc
     3541 ttcgagacgc tggacgaagt ggccgatgcc ctggcgctgg acgtggtgcc gatgtcatgg
     3601 ccggtcggaa tgggcgggac ttttcaaggg gttctcgact tcgcgacggg catggtgtcg
     3661 aggcccgagg gtgacagccg cgagttcctc ggcaaggtcg aggaaatggc gctgccgcaa
     3721 gacgtggccg aggaggtcga actggcccgg ggcggttatc ccgagttcag ccttgacgcc
     3781 tatcgtcacg gcgacatgac cccagcctat ttcggctcgg ccctgaagaa ttttggcgtg
     3841 gctgaactga ttgacgccat cgccaaattc gccccgccgc cgcgcccgca aagctccgac
     3901 caaggcacga tcagccccga tcgcgccgac gtgacgggct tcattttcaa ggtccaggcc
     3961 aacatggatc cgcagcatcg cgaccggatc gccttcatgc ggatggtctc ggggactttc
     4021 aggcgcggga tgaagctgac cccatccggg ctcggaaagc cgatcgccgt gcactcgccc
     4081 atcctgtttt tcgcgcagga ccgtgagatt gccgatacgg cggaagccgg tgatatcatt
     4141 gggattccca atcacgggac gctgcgggta ggtgatacac tgagcgaaaa gaacgacgtg
     4201 cggttcaccg gcctgcccaa cttcgcaccg gaaatcctgc ggcgtgtggc gctgaaggat
     4261 ccgaccaaga ccaagcaact gcgcaaggcc ctcgacgacc tgtccgaaga gggtgtgatc
     4321 caggtgttct atccggagat cggggcgcag tggatcgtcg gagttgtcgg ccaattgcaa
     4381 ttggatgtgc tgatttccag gctcgaggcg gagtacaagg tcgaggccgt gctcgagcct
     4441 tctccgttcg acacggcgcg ctggctcaag ggaaccgatg tggcgctcaa gagctttgcc
     4501 gagttcaaca agtccaacct ggccaaggac cgcgacggcg acccggtgtt catggccagg
     4561 tcggcctggg acgtcagcta tcagcaggaa aagaacccgg agcttggctt cagcgcaaca
     4621 aaggaacgct gacggccaat tgcatcgggg ttgcaacgca actgcaaggc ttcacaatgc
     4681 cgcgatggaa ctgacggtcg cctcctacaa catccacaag gcggtggggc tcgaccgccg
     4741 ccgcgatccc gagcgcatca tggccgtgct gcgcgaaatc gatgccgacg tgatcgccct
     4801 gcaggaggct gaccgccggt tcggcgaccg ggtcagcgtg ctgccgcggc aagtgctgga
     4861 ggaccacggg cactggcagg ccgtggcgct cggccagcga cctgattcga tcggctggca
     4921 cggcaatgcc gtgctggtgc ggcgggggat cgaagtggtc gacgccgata tcgtgccgct
     4981 gcccacgctg gagccgcgcg gggcagtgcg ggtggacctg gtcaagcacg ggcagccggt
     5041 acgggtcgtg ggcatgcatc tcgatctgtc gggcctgcgc cggcgccagc aggtgcgcac
     5101 ggtgctggcc catgtcgaga tctgcggcga ggcctgccct accgtgctga tgggcgac
//
LOCUS       DATNBV010000466         2272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3081977,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000466 DATNBV010000000
VERSION     DATNBV010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3081977"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VN627_11035"
     CDS             <1..522
                     /locus_tag="VN627_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXD03830.1"
                     /translation="IISIQGLTMSTSARIGGDRMDEAITAAVRRNHNLLIGEATAERV
                     KQELGTARMPADGTGGRVVVKGRNVTRGVPSEVEITEAELAEALAEPVGQIVHAVRLA
                     LENTLPEIAADMIDGGITMTGGGSLLREIAPVLADATGLAVMVADDPLHCVALGAGRA
                     LEDLTYRGVLHRA"
     gene            complement(525..1187)
                     /locus_tag="VN627_11040"
     CDS             complement(525..1187)
                     /locus_tag="VN627_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXD03831.1"
                     /translation="MIAPRRALLAAALCWIGFAVIVALVETGHGARLDAAGLTVWRQS
                     DRPGLPRGPAWLLEAARDFTALGGPLLRNLFALAAVAALLFLRERRRAVLFALTVVLA
                     WVVDAALKALVGRARPALVPHLMAAGGNSFPSGHSFNAAVVYIAMALVFAGFSSRAAV
                     RRTVIGAALILTLLVALTRVWLGVHWPSDALGGWLGGAGWAFLAAAVLRPPARAIAEA
                     AG"
     gene            complement(1184..2074)
                     /locus_tag="VN627_11045"
     CDS             complement(1184..2074)
                     /locus_tag="VN627_11045"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXD03832.1"
                     /translation="MDQSALPIVINRSGGTATALGEGLGQAVEKAFAQAGLPVALHLV
                     DGSALSQTLAGLAGSPVVAVGGGDGTLGHAAGLLAAAGQAMAILPLGTRNHLARQLAI
                     PDDLEGAARVIAAGRRQRIDLARAGDRIFVNNASLGLYARLVRDREAVVARAHAPKWL
                     ANIPAAWHVLAHLRSHPLDLEIDGERHLIATPLLFIGNNRYLLSAGEVGTRDSLSDGE
                     LSFYAVAARTPLQLLAMALRTLIGRGDPARDFAALTTGRRATLRGRPHLDVACDGEVA
                     RMALPLDLEIMPGALEVVVP"
BASE COUNT          393 a          786 c          828 g          265 t
ORIGIN      
        1 atcatctcga ttcaggggct gacgatgagc acctcggccc ggatcggcgg ggaccggatg
       61 gacgaggcga tcaccgccgc cgtgcgccgc aaccacaacc tgctgatcgg cgaagcgacc
      121 gccgaacggg tcaagcagga gctcggcacg gcccggatgc cggccgacgg gaccggcggg
      181 cgggtcgtcg tcaagggccg caacgtgacc cgcggcgtgc cctcggaagt ggagatcacc
      241 gaggcggagc tggccgaggc gctggccgag ccggtcggcc agatcgtcca cgccgtgcgc
      301 ctggcgctgg agaacaccct ccccgaaatc gccgccgaca tgatcgacgg cggcatcacc
      361 atgacggggg gcggctcgct cctgcgcgag atcgccccgg tgctggccga tgcgacgggg
      421 cttgcagtga tggtggccga cgatccgctg cactgcgtgg ccctgggcgc cgggcgggca
      481 ctggaggacc tgacctatcg cggggtcctg caccgcgcct gaggtcagcc ggccgcctcg
      541 gcaatggccc gggcgggcgg gcgcagcacg gccgcggcca ggaacgccca ccccgccccg
      601 ccgagccagc cgccgagcgc atcgctcggc cagtgcacgc ccagccagac ccgcgtgagc
      661 gcaaccagca gggtgaggat cagggccgct ccgatcacgg ttcggcgcac tgcggcacgg
      721 ctactgaacc cggcgaagac cagcgccatg gcaatataga ccaccgcggc attgaagctg
      781 tggccgctgg ggaagctgtt gccgcccgcg gccatcagat ggggcacgag tgctggccgc
      841 gcccggccga ccagggcctt gagcgcagcg tccacaaccc aggccagaac caccgtaagc
      901 gcgaacagga cggcccggcg tcgctctcgc aggaacagga gggctgccac ggcagcaagc
      961 gcaaacaggt tgcgcagcag cggtccgccc agggccgtga aatcgcgcgc tgcttccagc
     1021 agccaggcgg gtccgcgcgg gagcccggga cggtcggact gccgccagac ggtgaggccg
     1081 gccgcatcga gcctcgcccc gtggcccgtc tcgacgaggg cgacaatcac ggcgaacccg
     1141 atccagcaca gcgcggcggc aaggagagct cggcgcgggg cgatcacggg acgacgactt
     1201 ccagcgcgcc cggcatgatc tcgagatcaa gcggcagggc catccgcgcg acttcgccgt
     1261 cgcaggcgac atcgaggtgg ggacggcccc gcagagtggc gcggcgcccg gtcgtcagcg
     1321 cggcgaaatc gcgcgccgga tcgccccggc cgatcagcgt tctcagcgcc atcgccagca
     1381 gttgcagcgg agtgcgggcg gcgacggcgt agaatgagag ttcgccgtcc gaaagcgagt
     1441 cccgcgtgcc cacctcgccg gccgagagga gatagcgatt attgccgatg aacagcagcg
     1501 gcgtggcgat gaggtggcgc tcgccgtcga tttcaagatc gagcggatgg ctgcgcaggt
     1561 gagcgaggac gtgccaggcg gccggaatgt tggccagcca cttgggcgca tgggcccgcg
     1621 ccaccacagc ctcccggtca cgcacgagcc gggcatagag cccgagcgaa gcgttgttga
     1681 cgaagatgcg gtcgccagcg cgagcaaggt cgatccgctg ccgccggccg gcggcgatca
     1741 cgcgcgccgc cccttcaaga tcgtcgggaa ttgccaactg ccgggcgaga tggttgcgcg
     1801 tgccaagcgg gagaattgcc atggcctgcc cggcggcggc cagcaggccc gcggcgtgcc
     1861 ccagggtgcc gtctccgccg ccgaccgcca ccaccgggct ccccgcaagc ccggcgagag
     1921 tctgcgacag ggcagagccg tcgacgagat ggagcgcaac cggcaggccg gcctgggcaa
     1981 aggccttctc gacggcctgg ccaagcccct cgccgagagc cgtggccgtg ccgccgctgc
     2041 ggttgatgac gatggggagc gcagactgat ccatgaggca actgccggaa cgcgcgaacc
     2101 ggccagatgg ccccccgtcc cgggcgtgtc acggatcaac ccgccagtgc agccggggtc
     2161 gcgcagtagc atccggcagg acatgaagga aagttgcctg ttggagacgc tgaaccgacg
     2221 gcaagtgtgg caaccttgat cctgcaattg caaaggctgt catcccgtct cg
//
LOCUS       DATNBV010000467         3848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1550536,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000467 DATNBV010000000
VERSION     DATNBV010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550536"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..78
                     /locus_tag="VN627_11050"
     tRNA            2..78
                     /locus_tag="VN627_11050"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Met,seq:cat)
     gene            complement(223..1389)
                     /locus_tag="VN627_11055"
     CDS             complement(223..1389)
                     /locus_tag="VN627_11055"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXD03833.1"
                     /translation="MHQDIRQQADICSHGQDLAFSAPGWSEGALRGGTSHHAVAPATP
                     GKFKLSGIEAARGIAAATVVFYHAARHLGQNYGPSVLLRLFQFGHAGVDLFFVISGFI
                     ILYVHFDDIGRPDRFGRYLARRLTRIYPTYWVALVLTVAMSFLGGLHGAPPWDRVVAS
                     VLLAPSSQAPLLGVAWTLQYEVFFYALFGILILHRRLGFAILLIWIGGIVGSQFGQSA
                     MGAIPNAIYNAFNFEFLLGMATAYLLRRHRIPAPRLVLGLGIALFVTAAAFENAGLFN
                     GYTDVSRLLYGLPAAMIVGGSAELDRLGLLNVPPALLVLGKASYSIYLFQFVFIGIVW
                     KAWLLAGLDTKLPHLASFPLLATAGVAGGVIMSRLVEYPLINLIRTVKRVRANA"
     gene            complement(1465..2520)
                     /locus_tag="VN627_11060"
     CDS             complement(1465..2520)
                     /locus_tag="VN627_11060"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXD03834.1"
                     /translation="MEPLWSLHYLRAVAALGVVLFHSLADTPWHFELGAAGIHLFFTI
                     SGFVIWTSTCRSPPSPKRFAMARVRRIVPLYWVATLIAILSTFVMPGYFWQATTRPVN
                     VLTSLLFVPHEAVSGGVFPVLYQGWTLQYEMYFYALFCICLLLPVKLRTSALCGWVVS
                     AVAIGAILDPPGAVLQTYTNPVCLEFVAGVVAAQLLSRLGAGTSPARWLALAFLGAVT
                     FLSASAWESQLGWTANLLIPLGAAATIVGMAGLERSGAMFRSTWLRRAGEASYSTYLF
                     QTLGFALVSAVLPHVPAVIRVILFVVSAQACGMAIAHYVEVPLLRLFRARPGQVGLEA
                     QSVTANLPVQDNSIILR"
     gene            2742..3737
                     /locus_tag="VN627_11065"
     CDS             2742..3737
                     /locus_tag="VN627_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXD03835.1"
                     /translation="MSLLAKAAGAVLAISAVWASAASAGEIALTFDDMPGIDLPNPAY
                     VAKVNRALLAGLRRHGYTATAFVNAGKYAELHDQRQRAFLRTWLAAGMDLGNHTYSHE
                     SLNDLGPAAFMADVIRGEPILRRALGRQHRSLRWFRFPDLETGADPATKQLVAGQLAA
                     RHYRSAPVTIDPDDWQFAEPYDEAVIHRNKARQAQIAAQYLAHLEAAIDWSRKASVAL
                     FGREIAFVILLHDSKLNADCFDALVTVLRRRDLQVVSLQRAMSDPAYATPDTYVGADG
                     IEWLERWSRTLGRDLPWDDYPDVPADIVAAYDRIEQDHPCPAAFHPSGCAAATNH"
BASE COUNT          798 a         1220 c         1211 g          619 t
ORIGIN      
        1 acgcggggtg gagcagcccg gtagctcgtc aggctcataa cctgaaggtc gcaggttcaa
       61 atcctgcccc cgcaaccaac accatcacaa accagccgca aaatactgca ggtgctcaac
      121 ggtggccgca acagacggcc ccgaggctcc gttcagcgca cgacaaccat cgcgcccgca
      181 tcctcgtcac cgatgatcgt agccacttcc ggatttcatc ggtcaggcgt tcgcccgaac
      241 cctcttcacg gtgcggatga ggttgatgag cggatattcg accaggcgcg acatgatcac
      301 gccgcccgca acgcccgccg ttgccagtaa agggaagctg gcaagatgcg gcagtttcgt
      361 gtccaagccg gccagaagcc acgctttcca tacgatcccg atgaacacga actggaagag
      421 gtagatcgag tacgacgcct ttcccagcac cagcagcgca ggagggacgt tcagcaggcc
      481 gagcctgtcc agttcggcgc tcccccctac gatcatggcc gcaggcaggc cgtacagaag
      541 ccggctcacg tcggtgtagc cattgaacag gccagcattt tcgaaagccg ccgcggtcac
      601 aaacagggca atgccaaggc caagcacaag gcgcggggcg gggatgcggt ggcggcgcag
      661 caggtatgcc gttgccatgc ccaacaggaa ttcgaaattg aacgcattgt aaatggcatt
      721 gggtatggcg cccatggccg attgcccgaa ttgcgagcca acgatgccgc cgatccatat
      781 caggaggatc gcgaagccaa gccggcgatg caggatcagt attccgaaca aggcgtagaa
      841 gaacacctcg tactgcagtg tccatgccac gccgagcagc ggcgcctgcg aggacggcgc
      901 aagcaggacc gaagctacca cgcgatccca tggcggagca ccgtgcaggc cgcccagaaa
      961 actcatggca accgtgagca caagggcgac ccagtaggtt ggataaatgc gggtgaggcg
     1021 ccttgccaga tagcgcccaa accgatccgg gcgcccgatg tcgtcgaagt ggacgtacaa
     1081 aatgatgaag ccgctgatta cgaaaaagag atcaaccccg gcgtggccga attgaaacaa
     1141 ccggagcaga acggatggtc cgtagttttg gccaagatgt ctcgcagcat ggtagaatac
     1201 gaccgtcgcg gcggcgatgc cacgcgccgc ttctatgccg gaaagcttga atttccccgg
     1261 ggtcgcaggg gcgacagcgt gatgggatgt accgccccgt aaagcaccct cggaccagcc
     1321 tggtgccgag aacgccaagt cctgcccatg cgagcagata tccgcttgct ggcgaatgtc
     1381 ctggtgcaaa ctcagcctcc ctcacgccgt cacaagggaa gcatgttgag tgactgctgg
     1441 caacgtccac ctgtcggccg ctgcctatcg caagatgatg gagttgtctt gaaccggtaa
     1501 attcgcggtg acggactgcg cctccaggcc gacctggcca ggccgggctc tgaacaaccg
     1561 cagcaatggc acttcgacat agtgggcaat cgccatgccg caggcctgcg ccgagaccac
     1621 gaacagtatc accctgatga cagcaggcac atgcggtagc accgcgctga ccagggcgaa
     1681 gcccagggtc tggaacaggt aggtcgaata ggatgcctcg ccagcccggc gcaaccaggt
     1741 gctgcggaac atcgcgccgg aacgctcaag accggccatg ccaacgatcg ttgccgctgc
     1801 gccgagggga atgagcaggt tcgcggtcca gccaagctga ctttcccagg cgctggcgga
     1861 caggaatgtc acggcgccga ggaatgccag tgccagccat ctcgcggggc ttgttccggc
     1921 accgagccgc gacagcagtt gcgcggccac cactcccgcc acgaattcca ggcagaccgg
     1981 gttggtatag gtctgcaaca cagccccggg aggatccagg atggcgccga tcgccacggc
     2041 cgagactacc caaccgcaca gggccgatgt gcgcagcttc accgggagca ggaggcaaat
     2101 gcagaacaag gcgtagaagt acatctcgta ctgcagggtc catccctggt agaggacggg
     2161 aaacactccg ccggagaccg cctcgtgagg gacaaacaga agcgatgtca gcacgttgac
     2221 tggccgggtg gtggcctgcc agaaatagcc gggcatcacg aatgtcgaca ggatcgcgat
     2281 cagcgtcgcc acccagtaga gcgggactat ccggcgaacc cgggccatcg cgaaccgttt
     2341 gggcgagggc gggctgcggc aggtgctggt ccagatcacg aagccgctga tcgtgaagaa
     2401 caggtgtatc cccgcggcgc cgagttcgaa atgccacggg gtatcggcaa ggctgtggaa
     2461 caggaccacg ccgagcgcgg caactgcgcg caagtagtgg agcgaccaca agggctccat
     2521 gttcggcgaa atgggccgcc ctgacccggc atccttgcgc aatctcgcct cggcaatatc
     2581 ggtctgccgg ccagtcggca tcctttgccg atataacttg ccccgcttat ggtgagcgtg
     2641 gcgctcgtgt taccgatagg taatttgcgc ttcgcggcgc ggtccggcag cggcaagggc
     2701 agcttggcgt gccgtttgtt ggatcggaca gggagttgcc attgtcattg cttgccaagg
     2761 ccgccggggc cgtcctggcg atttccgccg tctgggcgtc ggcggcatca gccggcgaga
     2821 tcgcgctgac cttcgacgat atgcccggca tcgatctgcc gaacccggcc tacgtggcca
     2881 aggtcaaccg tgcattgctg gcgggacttc ggcgacatgg ctacacagcc acggccttcg
     2941 tcaatgccgg caagtacgcc gaattgcatg accagcgcca acgcgccttc ctgcggacgt
     3001 ggctcgccgc gggcatggat ctgggcaacc acacctattc gcatgaatcg ctgaacgacc
     3061 ttggccccgc agccttcatg gcggacgtga tcaggggtga gccaatcctg cgccgagccc
     3121 tggggcgcca gcaccgtagc ctgcgctggt tccggtttcc ggaccttgaa accggcgcgg
     3181 acccggcgac caaacagctg gtcgccggac agcttgcggc gcgccactat cgctccgcac
     3241 ccgtaaccat cgatcccgac gactggcagt tcgccgaacc ctacgacgaa gcggtgatcc
     3301 atcgcaacaa ggcgcgccag gcgcagattg ccgcccaata cctggcccac ctcgaggcgg
     3361 cgattgattg gtcgcgcaaa gcgtcggtcg cgcttttcgg cagagagatc gcctttgtca
     3421 ttctcctcca cgacagcaag ctgaatgccg actgtttcga cgcgctggtc acggtgctgc
     3481 gccggcgcga tctgcaggtc gtgagcctcc agcgggcgat gagcgatcca gcctatgcta
     3541 cccccgatac ctatgttggc gcggatggca tcgaatggct cgagcgctgg tcacgcacgc
     3601 tcggccggga tctcccgtgg gacgattatc cggacgttcc agctgacatc gtcgctgcat
     3661 acgaccgtat cgaacaggat cacccgtgcc cggccgcgtt ccatcccagc ggctgcgccg
     3721 cggcgactaa tcattgaatg aagacccgtc tcctgccgtg cgccggtgaa tggctccacc
     3781 gcctcggcgg ccctgatcgg cggggagggc tactgggccg cgcgtgcggc aacggcggtc
     3841 gatgcggg
//
LOCUS       DATNBV010000468         3403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2469775,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000468 DATNBV010000000
VERSION     DATNBV010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2469775"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VN627_11070"
     CDS             <1..221
                     /locus_tag="VN627_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXD03836.1"
                     /translation="GEPATFANGGTILLAASDEAAVDAWHAAGLAHGGTCEGPPGPRA
                     QIGGRYGAYLRDPDGNKLGCYVGNLFGA"
     gene            complement(227..817)
                     /gene="metW"
                     /locus_tag="VN627_11075"
     CDS             complement(227..817)
                     /gene="metW"
                     /locus_tag="VN627_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HXD03837.1"
                     /translation="MSAALRPDLAIIADHVPAGASVLDVGCGDGELLAALTAKGCDAR
                     GMELDPHNVAECVARGLSVIQGDADTDLAFYPDKSIDYAVLSQTLQTTKRPDLVLDEL
                     LRVGRKAIVSFPNFAHWRVRLSLLWGGRMPVTRLLPVAWYETPNIHHLTIADFRAHLK
                     ERGIRVEQAWFLKGDRRTGAAAANFRAEHAVFLLSR"
     gene            complement(814..1926)
                     /locus_tag="VN627_11080"
     CDS             complement(814..1926)
                     /locus_tag="VN627_11080"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HXD03838.1"
                     /translation="MASAPFLTTSRVLTLAQPLALDGGQQLDGVRIAYETYGTLNAER
                     TNAILICHALTGDQHVASPHPITGKPGWWVRMVGPGKPIDTDRYHVICANVLGSCMGS
                     TGPASEAPDGKPWGMRFPVITIRDMVRGQVALLDALGIARVHAVVGGSMGGMQALSFA
                     ANFPDRCAAALVIAATARHSAQNIAFHEVGRQAIMADPQWRDGDYYGTKGGKGPEKGL
                     AVARMAAHITYISEAGLTEKFGRNLQDREAKTFGFDADFQVESYLRYQGLSFTDRFDA
                     NSYLYITRAMDYFDLAEEHGGRLADAFAKSSARFCLVSFDTDWLYPTAESRLIAHALN
                     AAGCPVSFMELSAPFGHDSFLLDVPALDRVVAGFLG"
     gene            2048..3148
                     /gene="hisC"
                     /locus_tag="VN627_11085"
     CDS             2048..3148
                     /gene="hisC"
                     /locus_tag="VN627_11085"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HXD03839.1"
                     /translation="MSGAPVPKSWISAIQAYVPGKSAGADGRPLIKLSANENPLGTSP
                     LALAARSEAAAAALYPDPDSKALRAALGELHGIDPALIVMGTGSDEILHIIAQGYAGP
                     GDEVIYVRYGFSVYDIAARRCGAVPVVAPDADYGTDVDALLALVTERTRVVFVANPNN
                     PTGSFLPKGEIARLHAALPGDVVLVIDQAYAEYVAPEDEDGAMALAAAHDNVLVTRTF
                     SKIYGLAGERIGWGTGAPGLVDTLNRIRGPFNVSATGQAMALAGVQDQAFVTAAAAHN
                     SAERARFVARLEALGNHGLRPLPSQANFVLILFEGALSAEAAFNGLAEAGYIVRWLPN
                     QGLPQALRITIGTSEQMDDIAAALTRLAEAAQ"
     gene            3145..>3403
                     /locus_tag="VN627_11090"
     CDS             3145..>3403
                     /locus_tag="VN627_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HXD03840.1"
                     /translation="MTIRSVAIVGLGLLGGSIGLAVREYLPGVATTGYDRDPATRSRS
                     AERGLVAQVCDNAAEAVREADLVIFCVPVGAMREAAAEVAGA"
BASE COUNT          529 a         1111 c         1244 g          519 t
ORIGIN      
        1 atggcgagcc ggccaccttc gccaatggcg ggacgatcct gctggcggcg tccgacgaag
       61 cggcggtgga tgcctggcat gccgccggcc tcgcccatgg cggcacctgc gaaggtccgc
      121 ccgggccgcg ggcgcagatc ggcgggcgct atggcgccta cctgcgcgat cccgatggca
      181 acaagctggg ctgctatgtg ggcaacctgt tcggcgcctg agcgcctcag cgcgagagca
      241 ggaagaccgc gtgttcggcg cggaaattgg cggctgcggc gccggtccgc cggtcgccct
      301 tgaggaacca ggcctgttcc acccggatgc cgcgctcctt caggtgggcg cggaaatcgg
      361 cgatggtcag gtggtggata ttgggcgtct cgtaccaggc gaccggcagc agccgcgtca
      421 ccggcatgcg cccgccccac agcagcgaga gccgcacccg ccagtgggcg aagttgggaa
      481 agctgacgat ggccttgcgg ccgacgcgca gcagctcatc cagcacgagg tcggggcgct
      541 tggtggtctg cagggtctgc gagagcacgg catagtcgat cgacttgtcg ggatagaagg
      601 caaggtcggt atcggcatcg ccctggatga ccgagagccc gcgcgcgacg cattcggcga
      661 cgttgtgcgg atcgagctcc atcccgcgcg cgtcgcagcc cttggcggtg agcgcggcga
      721 gcagttcgcc gtcgccgcag ccgacatcga gcacgctggc accggccggc acgtgatcgg
      781 cgatgatcgc gagatcgggg cgcagggcgg cgctcatccg aggaaccccg cgacgacccg
      841 gtcaagcgcc ggcacgtcga gcaggaagct gtcatgtccg aacggggccg acagttccat
      901 gaagctcacc gggcaaccgg ctgcgttgag cgcatgggcg atcagccgcg attcggccgt
      961 gggatagagc cagtcggtat caaaggagac gaggcagaag cgggcgctgc ttttcgcgaa
     1021 ggcatcggcg aggcggccgc cgtgttcctc ggccaggtcg aaatagtcca tcgcccgggt
     1081 gatgtagagg taggaattgg catcgaaccg gtcggtgaag gagagaccct ggtagcgcag
     1141 gtagctctcc acctggaaat cggcatcgaa gccgaaggtc ttggcttccc ggtcctgcag
     1201 gttgcggccg aacttctcgg tcaggcccgc ctcggaaata taggtgatgt gcgcggccat
     1261 gcgggcgacg gcgaggccct tctccgggcc cttgccgcct ttcgtgccgt agtaatcccc
     1321 gtcccgccac tgcggatcgg ccatgatcgc ctggcggccg acttcgtgga aggcgatgtt
     1381 ctgggccgag tggcgcgccg tggcggcgat gaccagcgcg gcggcgcagc gatcggggaa
     1441 attggcggca aagctcagcg cctgcatccc gcccatcgag ccgccgacga ccgcgtgaac
     1501 ccgcgcgatt ccaagagcat cgagcagggc aacctgcccg cgcaccatgt cgcggatggt
     1561 gatgaccggg aaacgcatgc cccagggctt gccgtccggc gcctcgctgg cggggccggt
     1621 cgagcccatg cacgaaccca gcacattggc acagatgacg tggtagcgat cggtatcgat
     1681 cggcttgccc gggccgacca tccgcaccca ccagccgggc tttccggtga tcgggtgcgg
     1741 gctcgccacg tgctggtcgc cggtgagggc gtggcagatc aggatggcgt tggtccgctc
     1801 ggcattcagg gtgccgtagg tttcataggc aatgcgcacg ccgtcgagct gctgcccgcc
     1861 atcgagagcg agcggctggg ccagcgtgag cacgcggctt gtcgtaagga acggggcaga
     1921 ggccatcagg cggcgggatt ggcgggattt ggggacgaaa gtcaatgggc gggtgggcgg
     1981 cggcttgccc gcgtggcgat tacggcaaag gttgcggccc ggcgcttggc cccttatggc
     2041 ccggcccatg agcggcgctc ctgtccccaa gagctggatc agcgcgatcc aggcctatgt
     2101 tcccggcaag tcggccggtg cggacgggcg tccgctgatc aagctctcgg ccaacgagaa
     2161 tccgctgggg acgagcccgc tggcgctggc ggcgcggagc gaggcggcag ccgcggcgct
     2221 ctatcccgat cccgacagca aggccctgcg tgccgcgctt ggcgaactac acgggatcga
     2281 tccggcgctg atcgtgatgg gcacgggctc ggacgagatc ctccacatca tcgcccaggg
     2341 ctatgccggc ccgggcgacg aggtgatcta cgtgcgctac ggcttttcgg tctatgacat
     2401 cgccgcccgg cgctgcgggg ccgtgccggt ggtggcgccc gatgcggact atggcaccga
     2461 cgtcgacgcc ctgctggcgc tggtcaccga acggacgcga gtagtcttcg tcgccaatcc
     2521 caacaatccg accggctcgt tcctgccgaa aggcgagata gcccggctcc atgcggcgct
     2581 gccgggcgac gtggtgctgg tgatcgacca ggcctatgcc gaatatgtcg cgcccgagga
     2641 cgaggacggc gcgatggcgc tggccgccgc tcacgacaac gtgctggtga cgcggacctt
     2701 ctccaagatc tacggccttg ccggggagcg gatcggctgg ggcaccgggg cgccggggct
     2761 ggtcgacacg ctcaaccgca ttcgcggacc gttcaacgtc tcggccacgg gccaggcgat
     2821 ggctctggcc ggcgtgcagg accaagcgtt cgtcactgcc gcggcggcac ataactcggc
     2881 cgagcgggcc cggttcgtcg cccggctgga agcgctcggc aatcacggcc tgcggccgct
     2941 gcccagccag gcgaatttcg tgctgatcct gttcgagggc gcgctcagcg ccgaggcggc
     3001 cttcaatggc ctggcggagg cgggctacat cgtccgctgg ctgcccaacc agggcctgcc
     3061 gcaagcgctg cggatcacca tcggcacctc cgagcagatg gatgacatcg cggcggcgct
     3121 gacccggctg gcggaagcgg cgcaatgacg atccgctcgg tcgccatcgt cgggctcggg
     3181 ctgctgggcg gctcgatcgg gcttgccgtg cgcgaatacc tgcccggcgt ggctacgacc
     3241 ggctacgacc gcgatcctgc gacccgttcc cgctcggcgg agcgcgggct cgtggcgcag
     3301 gtctgcgaca acgcggccga agccgtgcgc gaggccgatc tggtgatatt ctgcgtaccg
     3361 gtcggcgcca tgcgcgaggc cgcggccgag gtggccggcg ccc
//
LOCUS       DATNBV010000469         2038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2469888,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000469 DATNBV010000000
VERSION     DATNBV010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2469888"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /gene="map"
                     /locus_tag="VN627_11095"
     CDS             complement(<1..697)
                     /gene="map"
                     /locus_tag="VN627_11095"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXD03841.1"
                     /translation="MSITYKDAEGIQGMRVAGKLASEVLDYLTPHIKPGITTMEIDRL
                     AAECMKKQGTTSATLGYQPAGYPPYPASLCTSVNHVVCHGIPNDKPLKKGDIVNVDVT
                     VIKDGWFGDTSRMYLVGEVSIAAKRLCALTYEAMWRGIVHVRPGIHLGDIGYEIQKFA
                     EGNGFSVVREFCGHGIGRNFHEEPQVLHYGKPGTLEELKPGMTFTIEPMINAGRKDVK
                     EFGSDGWTIVTKDH"
     gene            887..>2038
                     /locus_tag="VN627_11100"
     CDS             887..>2038
                     /locus_tag="VN627_11100"
                     /inference="COORDINATES: protein motif:HMM:NF037184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03842.1"
                     /translation="MTLHITEFEGGNALSDFRVQQLLPRLQAIDSKVSGVSARFVHLV
                     AADHAPTPAERERLAALLTYGEPCRRSVGGALIVVTPRFGTVSPWASKATDIAHNCGI
                     ALRRVERIAEFHIGLKKGGLDPAQRLAIADLLHDRMTESVMFERVAARGLFTGIEPAP
                     MEYVDVLAGGRSALEKANTQFGLALAEDEIEYLVEAFKALGRNPSDVELMMFAQANSE
                     HCRHKIFNASFAIDGVAQERSMFGMIRHTHQVSPQHTVVAYSDNASVMEGGKVERFIA
                     RPASASRYEKESAVDHVLMKVETHNHPTAISPFPGASTGAGGEIRDEGATGRGSRPKA
                     GLTGFTVSKLWGGNFGKPGHIASPLQIMVEGPLGGAAFNNEFGRPCLLGY"
BASE COUNT          360 a          664 c          647 g          367 t
ORIGIN      
        1 aatggtcctt ggtgacgatg gtccagccgt cgctgccgaa ttccttcacg tccttgcgcc
       61 ccgcgttgat catgggctcg atcgtgaacg tcatgccggg cttgagctcc tccagcgtgc
      121 cgggcttgcc gtagtgcagc acctggggct cctcgtggaa gttgcggccg atgccgtggc
      181 cgcagaactc gcgcaccacc gagaagccgt tgccctcggc gaacttctgg atctcgtaac
      241 cgatgtcgcc caggtggatg cccggcctca cgtgcacgat gccgcgccac atcgcctcat
      301 aggtcagcgc gcacagccgc ttggccgcga tcgacacttc gccgaccagg tacatgcggc
      361 tggtgtcgcc gaaccagcca tccttgatca ccgtgacgtc gacgttgacg atgtcgcctt
      421 tcttgagcgg cttgtcgttg ggaatgccat ggcacaccac gtggttgacc gaagtgcaga
      481 gcgatgccgg atagggcgga taacccgccg gctgatagcc cagcgtggcc gaggtggtgc
      541 cctgtttttt catgcactcc gccgccagcc ggtcgatctc catggtggtg atgcctggct
      601 tgatgtgcgg cgtgaggtaa tccagcacct cggaggcaag ctttcctgcc acccgcatgc
      661 cctggatacc ctcggcgtct ttgtaggtaa tgctcatgac ctggcgcttg gtaaaacggc
      721 gattatccca gagggtgaaa tcccttgcgt tggcaactgg cggcttcgcg gcggccctgc
      781 gggtgcggca gggctcaagt cgccctagtt aaaatatgct ttcagcacca gggccccagt
      841 cagcggcctg ccgttaccgt ttccccgacc acacaggctt gacattgtga ccctgcacat
      901 caccgagttc gagggcggca acgccctcag cgacttcaga gttcagcagc tgcttccccg
      961 gctgcaagcc attgacagca aggtcagcgg cgtcagcgcc cgattcgtgc acctggtcgc
     1021 cgccgatcat gcacccaccc cggcggaacg ggaacgcctg gcggcactgc tgacctacgg
     1081 ggagccctgc cggcgttccg tggggggagc gttgatcgtc gtcaccccgc ggttcggcac
     1141 cgtttctccg tgggcctcga aggccaccga catcgcccac aactgcggca tcgcgctcag
     1201 acgagtcgag cgtatcgccg aattccacat cggcctgaaa aagggcgggc tggacccggc
     1261 gcagcgtctg gcaatcgcgg atctgttgca cgaccgcatg accgaaagcg tgatgttcga
     1321 gcgcgttgcc gcgcgcggcc tgttcaccgg catcgaaccg gcaccgatgg agtatgtcga
     1381 tgtcctggcg ggcggccgct cggcgttgga aaaggccaac acccaatttg gcctggcgct
     1441 ggccgaggac gagatcgagt acctggtcga ggctttcaag gccctgggcc gcaacccgag
     1501 cgacgtcgaa ctgatgatgt tcgcgcaggc caacagcgag cactgccgcc acaagatctt
     1561 caacgcctcg ttcgcaatcg acggcgtggc gcaggaacgc agcatgttcg gcatgatccg
     1621 ccacacccac caggtgagcc cacagcatac ggtcgtcgcg tattccgaca acgcatcggt
     1681 catggaaggc ggcaaggtcg agcgcttcat tgcgaggccg gccagcgcgt cgcgctacga
     1741 gaaggaaagc gccgtcgacc acgtgctgat gaaagtggaa acccacaacc acccgacggc
     1801 gatctcgccg tttcccggcg cctccaccgg ggccggaggc gagatcaggg acgagggcgc
     1861 gaccggccgg ggctccaggc cgaaggcggg cctgacgggt ttcacggttt ccaagctgtg
     1921 gggcggcaat ttcggcaagc ccgggcacat cgccagcccg ttgcagatca tggtggaagg
     1981 gccactcggt ggcgcggcct tcaacaatga attcggccgg ccctgcctgc tcggctat
//
LOCUS       DATNBV010000470         7916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1253036,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000470 DATNBV010000000
VERSION     DATNBV010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253036"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(506..895)
                     /locus_tag="VN627_11105"
     CDS             complement(506..895)
                     /locus_tag="VN627_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03843.1"
                     /translation="MDGDRSLAEYGAYAAAPVARLTCDPLRQATILWRNWPVTGTMPL
                     AVTTTGTRRLLTASTDGAGGAQVSLPARDPLLDAMAFSRGRFMLEMPGSPTLYLPAWP
                     ELSRVIEDCRQKLDGAPGAAPEASPKP"
     gene            1159..1941
                     /locus_tag="VN627_11110"
     CDS             1159..1941
                     /locus_tag="VN627_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HXD03844.1"
                     /translation="MPDPSQPRPAEKPARKAPKQRVDQLLVERGLAESRTRAQALVLA
                     GLVFSGETKLAKPGQPLPADAPLEVRGRDHPWVSRGGIKLAHALDHFHLDPAGAVAMD
                     IGSSTGGFTDVLLQRGAAHVFAVDTGTNQLAWKLRQDPRVTVLEQTSARVLTPDLIDR
                     PCNWVVCDASFIGLAKVLEVPLRLAAPACRLVALIKPQFEVGKGEVGKGGVVRDPALH
                     RRVCDEVRDWLEQGGWTVDGIVESPITGPEGNVEFLVSSHRG"
     gene            2068..2622
                     /locus_tag="VN627_11115"
     CDS             2068..2622
                     /locus_tag="VN627_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HXD03845.1"
                     /translation="MNQLASTGQLRASLLRWALVLVPAVVLLGFLSAQIGGSGPDNPW
                     FAGLDKPVIYPPPATFGIVWTVLYVLMGIAAAMIGSARGARGRGLALGVFAVQLLANL
                     AWTPVFFGFHLISAGLGIIVALDLLVLLTVVLFWRVRPLAGMLLLPYLAWVLFATVLN
                     WQFLQLNPNADGMPTANTVTRVRL"
     gene            2660..2902
                     /locus_tag="VN627_11120"
     CDS             2660..2902
                     /locus_tag="VN627_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HXD03846.1"
                     /translation="MQSDNPLFADFAKLMNSAAGTFAGMGREARDAARERFKEAFGGF
                     DFVTREEFDAVKDMAAKAREEVEDLKARLSALEAKG"
     gene            complement(3121..4020)
                     /gene="rpoH"
                     /locus_tag="VN627_11125"
     CDS             complement(3121..4020)
                     /gene="rpoH"
                     /locus_tag="VN627_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HXD03847.1"
                     /translation="MSKQRNLPVVPALGGEASLNRYLSEIRKFPVLTAEQEYMLAKRF
                     SEHGDTDAAAQLVTSHLRLVAKIAMGYRGYGLPVSELISEGNIGLMQGVKKFEPDRGF
                     RLATYAMWWIKASIQEFILRSWSLVKMGTTAAQKKLFFNLRRMKKNLDAYEDADLKPD
                     DLRKIATDLGVSEQEVINMNRRMMMGGDASLNVSMRDDSESQWQDLLADDRPLQDETV
                     ADAEEARYRRELLTEAMGSLNDRERQILTDRRLAEDPKTLEELSQVYNVSRERVRQIE
                     VRAFEKLQKAIHRIATERHLLPA"
     gene            complement(4114..5070)
                     /locus_tag="VN627_11130"
     CDS             complement(4114..5070)
                     /locus_tag="VN627_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXD03848.1"
                     /translation="MGGKDSILDGVIATGGQRLDRALADATGLSRERIKALLGEGRVL
                     LGGKAVSLASLKPEAGTAFAIAVPEAAPAEAAAQDIPLSIVFEDEHLIVIDKPAGLVV
                     HPAAGNLDGTLVNALLHHCAGQLSGIGGVARPGIVHRIDKDTSGLLVVAKCDAAHEGL
                     ARQFADHSIERAYLAITAGTPSPLAGTVRGNIGRSDSNRKKMALVGEGRGKHAVTHYK
                     VLEALGDAALVECRLETGRTHQVRVHLASIGHALLGDPVYGRTPSRLRPTLQKLDFHR
                     QALHAAVLGFIHPTLGTALRFESPVPSDMRELLRELGSNRSE"
     gene            5196..6176
                     /locus_tag="VN627_11135"
     CDS             5196..6176
                     /locus_tag="VN627_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXD03849.1"
                     /translation="MDQSPPSRHLVHPSLEPALDSLPEFDLAAGDLAQVRAGMVEARV
                     AAAAAASAGTSPAGGVAPTEQIVPGPGGRDVRVLIWRSPGASGALPAVLHIHGGGFVM
                     GSPDMSAADHAAFVAQTPCVLVAVEYALAPEATHPAAVEDCYAALRWMAEGAGELGID
                     PTRIGVSGVSAGGGLAAALALLARDRGEIGLAFQHLIYPMLDDRTGSVGEDHPFAGHY
                     VWPATNNQFGWRALLGQEPGGPAVSPYAAAARAENLAGLPPAFISVGALDLFAEENLD
                     YARRLMRAGVPVELHVYPGAFHGFHLVREAEITRQALRDEEAALVRFLGG"
     gene            6370..7329
                     /locus_tag="VN627_11140"
     CDS             6370..7329
                     /locus_tag="VN627_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXD03850.1"
                     /translation="MSLPIDPIELVDPALREGLALLPALDFSKVGLPEVRQALAAMRL
                     AEPPPAGALMPEERFAPGPAGRQVRILVWRTPGATGPLPAVLHMHGGGYVLGSPDFGE
                     ENHRALVETLPCMVVAVQYALAPEQPHPQPVEDCYTALRWMSENAAELGIDRARIGVS
                     GVSAGGGLAAALALLARDRGEIPLAFQHLIYPMLDDRTCTAEDPHPHTGHWCWNNDSN
                     HFGWSALLGVPPGSEGVSPYAAPARATDLAGLPPTYLSVGMLDLFLEENLEYARRLSR
                     AAVPVELHAYPGAYHSFDNFGDVPVAAQARRDNNEALMRFLRG"
     gene            7326..7730
                     /locus_tag="VN627_11145"
     CDS             7326..7730
                     /locus_tag="VN627_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HXD03851.1"
                     /translation="MSEWGAVLAQAARETMLEFARAAFPREACGLLLGEGGQIRAATF
                     APNVHPEPLRHFEIDPAALIAAHKAAREGGPQVLGYWHSHPNGLAAPSDTDRAHASGE
                     GRVWAIVANGAISLWVDAPNGFAALSYAVQDG"
     gene            7835..>7916
                     /locus_tag="VN627_11150"
     CDS             7835..>7916
                     /locus_tag="VN627_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase"
                     /protein_id="HXD03852.1"
                     /translation="MNDTSLDLAALLCSRLCHDMLSPVGAL"
BASE COUNT         1209 a         2760 c         2593 g         1354 t
ORIGIN      
        1 aaggacctgt ctgcggccga ccagaagaag gtcatgtcga gcaacctcaa gaacctgctc
       61 gaagcccacc agtgatggcg gcggcgtttg gttcttcccc ccgttatggg ggagggtgag
      121 gggcgcgtct gccccccaaa cctccaacca aaatgagaag ggcagcaacg cccgtcagcg
      181 tgctgccccc aatgagcttc aaaccaaaat gagaagggca gcaacgcccg tcagcgtgct
      241 gcccttcgca tggtcagcgg ccggaagtgc cgcccacgaa gcctttatcg ctcgatcacc
      301 ggttagcgat aatgctccgc gctgcggaat gccgacctct ctgctggacc atgagacatc
      361 ggatcacctc ctttcgcgct gttgtgccaa agcgcccgtc tccgggctgg ggccgccgcc
      421 gcgtgttgcg ctgcaacctt gacacccaag gtattgattc cgtccgctac gacaagactt
      481 gcatgaattt tttttgccgt cagaatcaag gcttcgggct tgcttccggc gcggctcccg
      541 gcgccccatc cagcttctgc cgacagtcct cgatcacccg gctgagctcg ggccaggccg
      601 gcaggtaaag cgttggcgaa cccggcattt ccagcatgaa tcggcccctg ctgaaggcca
      661 tggcatccag cagcgggtcg cgggcgggca gactcacctg ggccccgcca gcgccgtcgg
      721 tgctcgcggt gagcaggcgc cgggtgcccg tggtggtcac cgccaggggc atcgtgccgg
      781 tgaccggcca gtttctccac agaatcgttg cctgcctcag cgggtcgcac gtcagcctgg
      841 ccaccggcgc ggcggcgtag gctccgtatt cggcgagcga gcggtcaccg tccatgcgcc
      901 agcgccaggt ccccggcgtc tgcggggcat cgcgccagtc ggctggcgcc ggcggagcgg
      961 atgcgagggg cggcggggct ggcgccgggg cgcgcggcgg tggggcagcc acgggcgggg
     1021 ccgatggtgc aacgcaggcg gccagcgcca atagcgggag tgtcgcgccg aggaagcgct
     1081 tcgcgtgatt tgccttcatc gccccggtat ggaatgagcc gcagcctccc gacaagagcg
     1141 caagcacgcc cgaggcccat gcccgacccg agccaacccc gtcctgccga gaagccggcc
     1201 cgcaaggcgc ccaagcagcg cgtcgatcag ctgctggtcg aacgcggcct cgccgaaagc
     1261 cgcacccggg cccaggcgct ggttctggcg gggctcgtgt tttctggcga aaccaagctc
     1321 gccaagcccg gccagccgct ccccgccgat gctccgctcg aggtgcgcgg ccgcgatcat
     1381 ccatgggtct cgcgcggcgg gatcaagctg gcccacgcgc tcgatcattt ccatctcgac
     1441 cccgccggtg ccgtcgcgat ggatatcggc agctccacgg gcggcttcac cgacgtcctg
     1501 cttcagcgcg gcgcggcgca tgtcttcgcg gtcgataccg ggaccaacca gctcgcctgg
     1561 aagctgcggc aggacccgcg cgtcaccgtg ctcgagcaga ccagcgcccg ggtcctcacg
     1621 cccgacctga tcgaccgccc gtgcaactgg gtggtctgcg atgccagctt catcggcctc
     1681 gccaaggtcc tcgaagtccc gctccgcctc gcggccccgg cttgccggct ggtcgccctg
     1741 atcaagccgc agttcgaagt gggcaagggg gaggtcggca agggcggggt cgtgcgcgat
     1801 cccgcgctgc accggcgggt ctgcgacgag gtgcgcgact ggctcgagca gggcggctgg
     1861 accgtcgacg ggatcgtcga gagcccgatc accgggcccg aagggaatgt cgaatttctc
     1921 gtcagttccc accgcggctg agacattttc aatgcgtccc gccgcggggc agccaaaagc
     1981 ctccgaagtg gaggaattgc cagtcgacgc gcgcccagcc aatatcgttt gggaacgcaa
     2041 ccgaatcgat caactccgcg aggatacatg aaccagcttg cgtcgaccgg ccagctcagg
     2101 gcctcgctcc tgcgctgggc gctcgtcctc gtccccgccg tcgtgctgct cggcttcctg
     2161 tcagcccaga tcggcggcag cgggccggac aacccgtggt tcgccggcct cgataaaccg
     2221 gtgatctatc cgcccccggc cacgttcggc atcgtatgga ccgtgctcta tgtcctcatg
     2281 ggcattgccg cggcgatgat cggctcggcc cgcggcgctc gcgggcgcgg gctggccctg
     2341 ggggtcttcg cggtgcagct cctcgccaac ctcgcctgga ccccggtgtt cttcggcttt
     2401 cacctgatca gcgccggtct cggcatcatc gtcgcgctcg atctgctggt gctgctgacc
     2461 gtcgtgctgt tctggcgggt gcgcccgctc gccggcatgc tgctgctgcc ctatctcgcc
     2521 tgggtgctgt ttgcgaccgt gctcaactgg cagttcctcc agctgaaccc gaacgccgat
     2581 ggcatgccca cggccaatac ggtgacgcgg gtgcgactgt aggcttgcac ggcgcgcctg
     2641 cccgcaccat gtagccccca tgcaaagcga caaccccctc ttcgccgact tcgccaagct
     2701 catgaacagc gcggcgggca ccttcgccgg catgggccgc gaagcccgcg acgccgcccg
     2761 cgaacgcttc aaggaagcct tcggcggctt cgacttcgtc acgcgcgagg aattcgacgc
     2821 ggtcaaggac atggcggcga aagcgcgcga agaagtggaa gaccttaagg cccgcctgtc
     2881 cgcgctcgaa gccaagggct gaggggcggc acgacgcaac ctacgtcatc ctgaacttgt
     2941 ttcaggatcc atggcctggg cttgcggaat gcacggggct tgacggctgg tgcagggctg
     3001 gcggcgggcg ccaccacatg gatgctgaaa caagttcagc atgacgggca ctccgtccgc
     3061 gcacacgaaa agggcggacc ttccggcccg cccttcgcat tccagcgctg atccgccgcc
     3121 tcaggccggc agcagatgcc gctccgtggc aatccgatgg atcgccttct gcagcttctc
     3181 gaaggcgcgc acctcgatct ggcgcacccg ctcgcggctg acgttataga cctggctcag
     3241 ctcttccagc gtcttcgggt cctcggccag ccgccggtcg gttaggatct gccgctcgcg
     3301 atcattgagc gaccccatcg cctcggtcag cagctcgcgc cggtagcgcg cttcctcggc
     3361 atcggccacg gtctcgtcct gcagggggcg atcgtcggcc agcaggtcct gccactggct
     3421 ctccgaatcg tcgcgcatgg agacgttgag cgaagcatcg ccgcccatca tcatgcgccg
     3481 gttcatgttg atcacttcct gctcggacac gcccaggtcg gtcgcgatct tcctgagatc
     3541 gtccggcttg agatcggcgt cctcgtaggc gtcgaggttc ttcttcatcc ggcgcaggtt
     3601 gaagaacagc ttcttctgcg ccgccgtggt gcccatcttc acgagcgacc acgagcgcag
     3661 gatgaattcc tggatgctcg ccttgatcca ccacatcgca taggtcgcga ggcggaagcc
     3721 gcgatcgggc tcgaacttct tcacgccctg catcaggccg atgttgccct cgctgatcag
     3781 ctcggacacc ggcaggccgt agccgcggta gcccatggcg atcttggcga cgagacgcag
     3841 gtggctggtc accagttgcg cggcggcatc ggtatcccca tgttcggaga agcgcttggc
     3901 gagcatgtat tcctgctcgg ccgtcagcac cgggaacttg cggatttccg acaggtagcg
     3961 gttgaggctt gcctcaccgc caagagccgg gaccaccggg agattgcgtt gcttgctcac
     4021 tttaccaacc cgtcctttcc gtgtcggccg gatttgtcga ccctagcagg catcaacggt
     4081 tccggccact gttcggggct atcaaaaaca taatcactct gatcggttac tgcccagttc
     4141 acgcagcagt tcccgcatgt ccgatgggac agggctctcg aaccggaggg ccgtgcccaa
     4201 agtcggatgg atgaagccga gcaccgcggc gtggagcgcc tggcgatgga agtcgagctt
     4261 ctgaagcgtg ggccgaaggc gcgaaggcgt gcgtccatag acagggtccc ccaatagcgc
     4321 atgaccgatt gatgcaaggt gaacgcgcac ctggtgggtt cgcccggtct ccagccggca
     4381 ttcgacaagg gcggcatccc ccagggcttc cagtaccttg tagtgcgtca cggcatgctt
     4441 gccgcgcccc tcgccgacga gcgccatctt cttgcgattg ctgtcggacc gcccgatgtt
     4501 tccgcgcacc gtgcccgcca gcggcgaagg tgtcccggcg gtgatcgcca gataggcccg
     4561 ctcgatcgaa tggtccgcga attgccgcgc cagcccctca tgcgcggcat cgcacttggc
     4621 gacgaccaac aggcccgagg tatccttgtc gatccggtgc acgatgcccg gccgcgctac
     4681 gccgccaatg cccgacaatt gaccggcaca atgatgcagc agggcgttga ccagcgttcc
     4741 gtcaagattt cccgccgccg ggtggaccac cagcccggcc ggcttgtcga tcacgatcag
     4801 gtgctcgtcc tcgaagacga tgctgagcgg aatgtcctgt gccgccgcct ctgccggcgc
     4861 ggcctcgggc acggcaatgg caaaagccgt tcccgcctcc ggcttgagcg acgctagcga
     4921 aacggccttg ccgcccagca atacccgtcc ctcgccgagc agggccttga tccgctcccg
     4981 gctgagcccg gtggcgtcgg ccagcgcccg gtcgagccgc tgcccgccgg tggcgatgac
     5041 gccatctaga atgctgtctt ttccccccat tatgccgccc atatggggag gctttgaagc
     5101 ggggcaagtg gcgccgtcgc agcgttgcgt tcgctacttt gccgacctat cctgcggcgc
     5161 aaggctccgg ccgtcacaac ccaggggaga tgatgatgga ccagtcaccg ccgagccgcc
     5221 acctggtcca tccgtcgctc gagcccgcgc tcgacagcct gccggaattc gacctggccg
     5281 ccggcgacct ggcccaggtg cgcgccggca tggtcgaggc gcgggttgcc gccgccgccg
     5341 ccgcctccgc cggcaccagc cccgcgggcg gtgtcgcgcc caccgagcag atcgttcccg
     5401 ggcccggcgg gcgcgatgtg cgggtgctca tctggcgctc gccgggggca tcgggcgcgc
     5461 tgcctgccgt gcttcatatc catggcggcg gctttgtcat gggctccccc gacatgagcg
     5521 cggccgacca cgctgccttc gtggcccaga ccccctgcgt actggtggcg gtggaatatg
     5581 cgctggcgcc cgaggctacc catcctgccg cggtggaaga ttgctacgcc gccctgcgct
     5641 ggatggccga aggggcgggc gaactcggca tcgatcccac gcgcatcggg gtgagcgggg
     5701 tgagcgcggg cggcggcctg gctgcggcgc ttgcgctgct ggcgcgggac cggggcgaga
     5761 tcgggctcgc attccagcat ctgatctatc cgatgctgga cgatcgcacc ggctcggtcg
     5821 gcgaagacca tccctttgcc ggccactatg tctggccggc aaccaacaac cagttcggtt
     5881 ggcgcgccct gctggggcag gagccgggcg gacctgcggt ttccccctac gctgccgccg
     5941 cccgtgccga gaacctggcc ggcctgcccc cggccttcat ctcggtgggg gcgcttgacc
     6001 tgttcgccga ggaaaacctc gattacgccc ggcgcctgat gcgggccggc gtgcccgtcg
     6061 aactgcacgt ctaccccggt gcgttccatg gcttccacct ggtgcgggaa gccgagatca
     6121 cccggcaggc gctgcgcgac gaggaggcgg cgctcgtccg cttcctcggc ggttgagcgc
     6181 tccggtcgag ccgctgcccg ccgatggcga tgacgccatc tagaatgctg tcttgtcccc
     6241 ccattatgcc gcccatacgg ggacggcgcg aggggcgaca agactgcctg gcgatcactg
     6301 gccagacggc ggcagttgct gtcctggccg cgacgcccta acttgcggtg accaaccggg
     6361 agaagcgatt tgagcctgcc aattgatccg atcgaactgg tggaccctgc cctgcgggag
     6421 ggactagcgc tcctgcccgc gctggacttc agcaaggtgg ggctgcccga agtgcggcag
     6481 gccctggccg ccatgcggct ggcggaacca ccgcccgccg gggcgctcat gccggaagaa
     6541 cggtttgcgc ccggacctgc gggacggcag gtgcggatcc tggtctggcg cacgccgggc
     6601 gcaacaggcc ccctgccggc cgtgctccac atgcacggcg gcggctacgt cctgggttcg
     6661 cccgattttg gcgaggaaaa ccaccgcgcg ctggtcgaaa ccttgccctg catggttgta
     6721 gccgtccaat atgccctggc gcccgaacag ccccacccgc agccggtcga ggattgctat
     6781 acggcgctgc gctggatgtc cgaaaatgcc gccgaactcg gcatcgatcg cgcccggatc
     6841 ggggtcagcg gggtcagcgc tggcggcggg ctggcggcag ccctggccct cctcgcccgc
     6901 gaccggggcg aaatcccgct cgccttccag cacctgatct atccgatgct ggacgatcgc
     6961 acttgcactg ccgaagatcc gcatcctcac accgggcact ggtgctggaa caatgacagc
     7021 aaccacttcg gctggtcggc cctgctcggc gtgccgcccg gcagtgaagg cgtatcgccc
     7081 tacgccgctc ctgcccgggc taccgacctt gccggactgc cgccgaccta tctctccgtg
     7141 ggaatgctcg acctgttcct cgaggagaac ctcgaatatg cccgccgcct cagccgggcc
     7201 gcggtgccgg tggaacttca tgcctacccc ggcgcctacc acagcttcga taatttcggc
     7261 gatgtcccgg tcgccgccca ggcccggcgc gacaataacg aggccctgat gcgcttcctg
     7321 cggggatgag cgaatggggg gcggtgctgg cgcaggcggc acgggagacc atgctcgaat
     7381 tcgcgcgcgc ggcctttcct cgcgaagcct gcggcctgct gctgggcgag ggcgggcaga
     7441 tccgggccgc taccttcgcc cccaacgtcc accccgaacc cctgcgccat ttcgagattg
     7501 atcccgccgc cctgatcgcc gcccacaagg ccgcgcggga gggcgggccg caggtgctgg
     7561 gctactggca ctcccatcca aacggccttg ccgcaccctc ggatactgat cgcgcccatg
     7621 ccagcggcga gggccgggtg tgggcaatcg tcgcaaatgg agcgatcagt ctgtgggttg
     7681 atgcgccgaa cggtttcgcg gcgctttcct acgcggtgca ggacggttaa ggcaggcgcg
     7741 cagggcgggt agccgggttc cgatttcggg ggctgggttt cgcggcctga accgtgtcaa
     7801 cttcgtcaac ttgtgcagca ggcccgccct tttcatgaac gatacctcgc tcgacctggc
     7861 cgccctgctg tgctcgcgcc tgtgccatga catgctcagc ccggttggtg cgctga
//
LOCUS       DATNBV010000471         2991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_577297,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000471 DATNBV010000000
VERSION     DATNBV010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577297"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VN627_11155"
     CDS             <1..385
                     /locus_tag="VN627_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HXD03853.1"
                     /translation="LAATLAAVALPAVPALGDPPPWAPAHGRRAHEEQAWDRGYDRGG
                     YENQDRYYERRLTRNDRIWRGNDGRYYCRRSNGTTGLVIGAAVGGVIGSQVAGRGDRT
                     LGAVIGALGGGLLGREVDRGNVRCR"
     gene            complement(434..1564)
                     /locus_tag="VN627_11160"
     CDS             complement(434..1564)
                     /locus_tag="VN627_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03854.1"
                     /translation="MPSPQIQFPANFTTASAIAYTNPDGTVQIVSADYPLPVTGGGGG
                     GGGTGGLTDTQLRAAPVTVSLASYSGLSDTQLRAAPVAVTLPASDWLTDTQLRANPVS
                     VSLASFGGLTDTQLRASALSVTPDAVADALSTSGSVTSATTVVSVATKGFGGGSFHVT
                     NAGTTCTITYEQSNDNTNWVALPVISLASASATPALTTTAAGIYGFASSAAFVRARVS
                     TYTSGTVAVTVAQKRQAPPVTGVSLAGGAAALGSVTVTGTATVSGNTNTGTGFTDSTT
                     ALAASATFTGTGRATTSFSQYAYFHAVAYADQAGTLFIDQSLDSGTTYQPVISQAVTA
                     STAAQLTTKITGAFAATTLYRVRFVNGAAAQATFRLSSAFSR"
     gene            1715..2452
                     /locus_tag="VN627_11165"
     CDS             1715..2452
                     /locus_tag="VN627_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD03855.1"
                     /translation="MTGGAKRIGALVCRAFAQAGWHVIVHYGRSREAAEVLARQLPSA
                     ETVSCDLADPVSAEAMVCGLASRLPDWRMLVNSAAVFNHDGADAISTEVFDEALAVNA
                     ASPARMTAAYLRHARSKGGRRVIDFLDQKLRNINPDFFSYTMAKSALEAAMRMQAMTV
                     PNPSDRIYGIAPGAMMASFDQADTEHEISGRMNLLKRLNQPAELADAALFLSEGWLAS
                     GSVLYIDSGQHLMRQPRDVLYLARGLR"
     gene            2535..>2991
                     /locus_tag="VN627_11170"
     CDS             2535..>2991
                     /locus_tag="VN627_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXD03856.1"
                     /translation="MWLLEKLLRGVVRTGRLTIIDHNGRPYEFGSGTTAPVVIRFTHP
                     GTAARIVRNPQLGAGEAYMDGHLVVEPPHDIRDLVMLVMGNAARAGGLDTPSRWRRLL
                     DSLLWRLDQANDRRRASKNVEHHYELTRQFYELFLDEDRQYTMGFWRSPD"
BASE COUNT          516 a          954 c         1027 g          494 t
ORIGIN      
        1 cctcgccgca accctggcag ccgtcgccct gcccgcggtc cccgcactcg gcgatccgcc
       61 accctgggca ccggcccacg gccgccgcgc acatgaggag caggcctggg accgcggcta
      121 cgatcgcggc ggctacgaaa accaggaccg ctactatgag cgccgcctga cccgcaacga
      181 tcgcatctgg cggggcaatg acgggcgata ctactgccgc cgcagcaacg ggacgaccgg
      241 gctggtgatc ggcgcagccg ttggcggcgt catcggcagc caggtcgcag gccggggtga
      301 ccgcaccctg ggcgcggtga tcggtgcgct tggcggcggt ctgcttggcc gcgaggtcga
      361 tcgcggaaac gtgcgctgcc gataaggggc gatgggccgg gcggcgcgac gatctcgccc
      421 ggccccgcgc cggtcagcgc gagaaagcgc tcgacaggcg gaaggtggcc tgggcggcgg
      481 ctccgttgac gaagcgcacg cggtacagcg tggtcgcggc gaacgcgccg gtgatcttcg
      541 tagtaagctg ggccgcggtt gatgccgtaa ccgcctggct gatgacgggc tgataggtag
      601 tgccgctgtc gagcgactgg tcgatgaaca gggttcccgc ctggtcagca tacgcaacgg
      661 cgtggaaata ggcatattgg ctgaagctcg tcgtggcccg gccggtcccg gtgaaagtgg
      721 ccgaagcagc cagcgccgtc gtcgagtcgg tgaaaccggt cccggtattg gtgttcccgc
      781 tgaccgttgc cgttcccgta acggtaacgc tgcccagcgc ggcagcgccg ccggccagcg
      841 atacaccggt aaccggcggc gcctgccgct tctgggccac ggtgaccgcg accgtgccgc
      901 tggtgtaagt cgatacgcgg gcccggacga aggccgcgct cgaggcgaag ccatagatgc
      961 cggcagcggt agtggtgagc gccggagtgg ccgaggccga ggccagcgag atcaccggga
     1021 gcgccaccca gttcgtattg tcgttcgact gctcgtaggt aatggtgcag gtcgtgccgg
     1081 cattggtaac gtgaaacgat ccgccgccga accccttggt cgccacggaa acgaccgtcg
     1141 ttgccgaagt gactgaaccg ctcgttgaaa gggcatcggc aaccgcatcg ggcgtcaccg
     1201 acagcgcgct ggcacgcagc tgcgtgtcgg tcagcccgcc aaagctggca agacttacgc
     1261 tcaccggatt ggcccgcagc tgggtgtcgg tcagccaatc ggatgcgggc agggtcaccg
     1321 cgaccggggc ggcgcgcagc tgcgtatccg acagaccgga atagctggcc aggcttacgg
     1381 tgaccggagc tgccctcagc tgggtatcgg tgaggccgcc ggtaccgcca cccccgccgc
     1441 cgccgccggt gaccggcagg gggtagtcgg cggacacgat ctgcaccgtc ccatcgggat
     1501 tggtgtaggc aatggcactg gccgtcgtga aattggcggg aaactgaatt tgcgggctcg
     1561 gcatgtcgcg gctctcctgg gaagcgattc gccgttcatg ccaattttgg aataggtagg
     1621 aaaatattgt ctcctatttg gttaaaagtg cagaggcctt ccgccggaag ccgtgctgtg
     1681 cttgaaaggt gtcatgacga aacccgcgat cctggtgacc ggcggcgcga agcgcatcgg
     1741 agccctcgtc tgccgcgcgt tcgcgcaagc aggatggcat gtcatcgtcc attacggtcg
     1801 ttcgcgcgag gcggctgaag tgctcgcaag gcagctcccc tcggctgaaa cagtcagctg
     1861 cgaccttgcc gatcccgtat cggccgaggc catggtgtgc gggcttgcca gtcggctgcc
     1921 ggactggcgc atgctggtga attccgcggc cgtgttcaac cacgatggcg ccgacgcgat
     1981 ctcgaccgaa gtcttcgatg aagcgctcgc ggtgaatgcc gcgtccccgg ctcgaatgac
     2041 cgctgcctac ctgcggcacg cccgcagcaa aggcggccgc cgcgtaatcg atttcctcga
     2101 tcagaagctg cgcaacatca atccggactt cttctcctac accatggcaa agtcggcgct
     2161 tgaggcggcc atgcggatgc aggcgatgac tgtgcccaac ccgtccgacc ggatctacgg
     2221 cattgccccc ggagccatga tggccagctt cgaccaggca gacaccgaac acgagatcag
     2281 tggccggatg aacttgctga aacggctcaa ccaaccggca gaactggctg acgcggcact
     2341 gtttttgagc gagggttggc tggctagcgg ctcggtgctc tacattgatt caggccagca
     2401 cctgatgcgc cagccgcgtg atgtgctcta tctcgcgcgc ggcttgcgct gaaccccacg
     2461 ggaacccgtt gggcccccgt gcgtttgggc ggctgaccgc aagtgcgggt tcgggacgca
     2521 ggggaatcgc aggcatgtgg ctgctcgaga agttgctgcg cggggtagtc cgcacggggc
     2581 gcctcaccat catcgatcat aatgggcggc cctatgagtt cggcagcggc acgaccgcgc
     2641 cagtggtaat ccgcttcact caccccggaa cggcggcgcg catcgtgcgc aatccccagc
     2701 tgggcgccgg ggaggcgtac atggacgggc atctcgtcgt cgagccgccg cacgacatcc
     2761 gcgatctggt gatgcttgtc atgggcaatg ccgcacgagc gggcgggctc gacacgccga
     2821 gccggtggcg ccggttgctg gactcgctcc tgtggcggct cgatcaggca aacgaccgtc
     2881 gccgggccag caagaatgtc gaacaccact acgaacttac gcgccagttc tacgaactgt
     2941 tcctcgacga ggatcggcaa tacaccatgg gcttctggcg ttcgcccgac g
//
LOCUS       DATNBV010000472         2046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3471803,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000472 DATNBV010000000
VERSION     DATNBV010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3471803"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VN627_11175"
     CDS             <1..751
                     /locus_tag="VN627_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HXD03857.1"
                     /translation="QYPDVLGRIPDLAAIAAAAQAQGALLVAVVTEPVALGLIEAPGK
                     LGADIVVGEGQSLGVGLQFGGPYLGLFGCREKFVRQMPGRLAGQTVDADGKRGFVLTL
                     STREQHIRREKATSNICTNSGLCALDFSIHMTLLGGEGLARLARLNHARARATAEALT
                     RVPGVELLSMGFVNELTLLLPRDAREVVRELAEYGVLGGVSLGRLYPQADGLHHALLV
                     TATETTTDDDIHALATILEAILVDTHEGAPT"
     gene            748..>2046
                     /gene="gcvPB"
                     /locus_tag="VN627_11180"
     CDS             748..>2046
                     /gene="gcvPB"
                     /locus_tag="VN627_11180"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HXD03858.1"
                     /translation="MSALNQSGWRPEMGGAGEVAPATQTASGDRGLMLEEPLIFELGR
                     PGTCGVDIEEPAGDPLPALARFLRAEPPALPGLTEPEAVRHYTRLSRQNYAIDLGPFP
                     LGSCTMKHNPRLNEKMARLPGFADVHPLQPQQTVRGAIGVIVELAQWLVTLTGMHSVA
                     MTPKAGAHGELCGLLCIRAALEARGDARAVVLVPESAHGTNPATAAFAGYRVESIPAT
                     VDGRVDIAALKARLGPDVAAVMITNPNTCGLFERDMKTISDAVHAAGGLVYCDGANFN
                     AIVGRVRPGDLGIDAMHINLHKTFSTPHGGGGPGSGPVVLSEALTPFAPLPFAQATAD
                     GTVRLIEEEDAPAEHPRSFGRMAAFHGQMGMFTRALTYILSHGADGLRQVAEDAVLNA
                     NYVLRSLDDVLDAPFGKTGPCMHEALFSDESLPEGFTTLDI"
BASE COUNT          308 a          802 c          626 g          310 t
ORIGIN      
        1 ccagtatccg gacgtgctcg gccgcatccc cgatctcgcc gccatcgccg ccgccgcgca
       61 ggcccagggc gcgttgctgg tcgccgtggt gaccgaaccc gtcgccctcg gcctgatcga
      121 ggccccgggc aagctcggcg ccgatatcgt ggtcggggaa gggcagtccc tcggggtcgg
      181 cctccagttc ggcggtccct atctcgggct gttcggctgc cgcgagaagt tcgtccgcca
      241 gatgcccggc cgccttgccg ggcagacggt cgacgccgat ggcaagcgcg gcttcgtgct
      301 caccctgtcg acccgcgaac agcacatccg gcgcgagaag gcgacaagca atatatgcac
      361 gaattcaggg ctttgtgcct tggacttctc aattcacatg acccttctcg gcggggaagg
      421 gctggcccgt ctcgcccgcc tcaaccacgc ccgcgcccgc gccaccgccg aggccctgac
      481 ccgggtcccg ggggttgagc tgctcagcat gggtttcgtc aacgaactca ccctcctcct
      541 cccccgcgat gcccgcgaag tcgtccgcga actggccgaa tacggcgtcc tcggcggcgt
      601 ctcgctcggc cgcctctacc cgcaggccga tggcctccac cacgccctgc tggtcaccgc
      661 caccgagacc accaccgacg acgatatcca cgccctcgcg acgatcctcg aagccatcct
      721 ggtcgatacc cacgaaggag ccccgacatg agcgcgctca accaatctgg ctggcgcccc
      781 gagatgggtg gcgcgggcga ggtggccccc gccacgcaga ccgccagcgg cgaccgcggc
      841 ctcatgctcg aggaacccct gatcttcgag ctcggccgcc ccggcacctg cggcgtcgac
      901 atcgaggaac ctgcgggcga tccgctgccc gccctcgcca ggttcctccg cgccgagccg
      961 ccggcgctgc ccggcctcac cgagcccgag gccgtgcgcc actacacccg cctctcgcgc
     1021 cagaactatg ccatcgatct cggcccgttc ccgctcggct cgtgcaccat gaagcacaac
     1081 ccccgcctca acgagaaaat ggcgcgcctg ccgggctttg ccgatgtcca cccgctccag
     1141 ccccagcaga cggtgcgcgg ggcgatcggc gtgatcgtcg agctcgccca gtggctggtc
     1201 acgctcaccg gcatgcattc cgtggccatg acccccaagg ccggcgccca cggcgagctg
     1261 tgcggcctgc tctgcatccg cgccgcgctc gaggcgcgcg gcgatgcccg tgcggtcgtc
     1321 ctcgtccccg aaagcgccca cggcaccaac cccgccacgg ccgcctttgc cggctaccgc
     1381 gtggaaagca tcccggcgac cgtggacggc cgggtcgaca tcgccgccct caaggctcgc
     1441 ctcgggcccg acgtggcggc ggtgatgatc accaatccca acacctgcgg cctgttcgag
     1501 cgcgacatga agaccatttc cgatgcggtc cacgccgccg gcgggctggt ctattgcgac
     1561 ggggcgaact tcaacgcgat cgttggccgg gtccgtccag gcgatctcgg catcgatgcg
     1621 atgcacatca acctccacaa gaccttctcc actccccacg gcggcggcgg ccccggttcg
     1681 ggcccggtag tcctgtccga ggcgctaacc cccttcgccc cgctgccctt cgcccaggcc
     1741 acggccgacg gcaccgtgcg gctgatcgag gaagaagacg cacctgccga acacccgcgc
     1801 tcgtttggcc gcatggccgc tttccacggc cagatgggca tgttcacccg agcgctcacc
     1861 tacatcctca gccacggcgc cgatgggctg cgccaggttg cggaggacgc ggtgctcaac
     1921 gcgaactatg tcctgcgcag cctcgatgat gtgctcgacg cgccgttcgg caagaccggc
     1981 ccctgcatgc acgaggcctt gttctccgat gagagcctgc cggaaggctt caccacgctc
     2041 gatatc
//
LOCUS       DATNBV010000473         2342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4386721,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000473 DATNBV010000000
VERSION     DATNBV010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4386721"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VN627_11185"
     CDS             <1..491
                     /locus_tag="VN627_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ C-terminal domain-containing
                     protein"
                     /protein_id="HXD03859.1"
                     /translation="EGKLTLLDQNRVIRPNKWFRLFATANTVGLGDTSGLYHGTQAIN
                     QGQMDRWNLVVGLNYLPHAVETEIVRKKVPELSEKLAADMVRVADLTRQGFMTGDIST
                     VMSPRTVITWAQNAQIFKDPGFAFRLSFLNKCDETERMLVAEYYQRVFGTDLPESVVG
                     RG"
     gene            617..1489
                     /locus_tag="VN627_11190"
     CDS             617..1489
                     /locus_tag="VN627_11190"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXD03860.1"
                     /translation="MDLRKLRHAVTLAEEGTFARAARRLNLSQPALTRSVQALETSLG
                     LRLFDRTGTGARPTTDGLRVLDHARTLLRQEASLRSEATLLARGESGKVAFGLGPMLT
                     PALGPVLAAILGGGARLDIRVEIEPVHVMEEMLLSEAIDFFIADAQRARAHPDLTVEP
                     LRTVPAGFYVRAGHPLAGMAEVVPADLASYPLASTALGTDLADLMAMQTVTCEVSPAL
                     KQAILVSDAVMIGMSIAVEPELSAGGIVRLRGDVLPVREARVGLVRRSSRTLTAGARR
                     VATTFDAVLAPYAG"
     gene            complement(1477..2226)
                     /gene="truA"
                     /locus_tag="VN627_11195"
     CDS             complement(1477..2226)
                     /gene="truA"
                     /locus_tag="VN627_11195"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXD03861.1"
                     /translation="MTRFALTLEFDGGPYMGLQRQAHGPSVQQAVEEAVLSVTGETVT
                     LHAAGRTDAGVHARAMRVHVDVAKDIGAFRLMEALNARLRLASEAIAVLDAEEKPDDW
                     HARFSCTGRRYVYRIINRRAPLTLDAGKAWNVKQPLDEAAMHEAAKALVGRHDFTTFR
                     STQCQADSPLKTLDRLAVRREGSEVLIEAAARSFLHHQVRSMVGCLALVGMGHWPVAR
                     MAEALAARDRTALGHNAPPEGLYFMGADYPA"
BASE COUNT          349 a          806 c          801 g          386 t
ORIGIN      
        1 ccgagggcaa gctgaccctg ctcgaccaga accgcgtgat ccgccccaac aagtggttcc
       61 gcctcttcgc cacggccaac accgtgggcc tgggcgatac atcggggctc tatcacggca
      121 cccaggcgat caaccagggc cagatggacc gctggaacct cgtcgtcggc ctcaactatc
      181 tgccgcatgc ggtggagacg gagatcgtcc gcaagaaggt gcccgagctg tcggagaagc
      241 tggcggccga catggtccgg gttgcagatc tgacccgcca gggtttcatg accggtgata
      301 tatcgaccgt gatgagcccg cgcaccgtga tcacctgggc ccagaacgcg cagatcttca
      361 aggacccggg cttcgccttc cgcctctcgt ttctcaacaa gtgcgacgag accgagcgga
      421 tgctggtggc cgaatactac cagcgcgtgt tcgggacgga cctgccggaa agcgtcgtcg
      481 ggcggggttg atcgcgcgcc ttgtggcgta gcggttggct gaacaggggt ttggccggtc
      541 gcccggcagc gccctttaac catgcgctcc gggcaaggcc ctgtcgtgat cgatgcggcg
      601 gtggtatcgc agcctcatgg atctgcgtaa gcttcgccat gctgttaccc ttgccgaaga
      661 aggcacattc gcgcgtgcgg cgcggcggct caatctgtcg cagccggcgc tcacgcggtc
      721 ggtccaggcg ctggaaacct cgctcggcct gcggttgttc gatcgtacgg ggactggtgc
      781 caggcccacg accgatggcc tgcgggtgct ggatcacgcc cgcaccctgc tgcgccagga
      841 ggccagcctg cgcagcgagg cgacgctgct ggcgcgggga gaatcgggca aggtggcctt
      901 tggactgggg cccatgctga ccccggcgct cggcccggtg ctggcggcga tcctgggcgg
      961 cggggcacgg ctcgatattc gcgtggagat cgagccggtc catgtgatgg aagaaatgct
     1021 cctgagcgag gcgatcgatt tcttcattgc cgacgcgcag cgggcgcggg cccatcccga
     1081 tctgacggtc gagccgctgc gcaccgtgcc cgccgggttc tatgtgcgcg ccggccatcc
     1141 cctggccggc atggccgagg tcgtccccgc cgatctggcc tcctatccgc tggcatctac
     1201 tgcgcttggc accgacctgg ccgatctcat ggccatgcag accgtcacct gcgaggtttc
     1261 cccggcgctc aagcaggcaa tcctggtgag cgatgccgtg atgatcggga tgagcatcgc
     1321 ggtcgagccc gaactgtcgg cgggcggcat cgtccgcctg cggggtgatg tcctgccggt
     1381 acgggaggcc cgcgtcgggc tggtccgccg ttcgtcgcgc acgctcacgg ccggggcgcg
     1441 gcgcgtcgct acgacgttcg acgcggtgct cgcgccttac gccgggtagt cggcgcccat
     1501 gaaatagagc ccttccggcg gggcattgtg gcccagcgcc gtgcgatcgc gcgctgccag
     1561 ggcctcggcc atgcgcgcaa cgggccagtg gcccatgccg accagcgcca ggcagccgac
     1621 catcgagcgg acctggtggt ggaggaacga gcgtgccgcc gcctcgatca gtacttcgct
     1681 gccctcgcgg cgcacggcca gccggtcgag cgtcttcagc gggctgtcgg cctggcactg
     1741 ggtggaacgg aaggtggtga aatcatgcct gccgaccagc gcctttgccg cttcgtgcat
     1801 ggccgcctcg tccagcggct gcttgacgtt ccaggccttg cccgcgtcca gcgtcagcgg
     1861 cgctcggcgg ttgatgatgc ggtagacata gcgccgcccg gtgcaggaga accgcgcatg
     1921 ccaatcgtcc ggcttctcct cggcatcgag caccgcgatg gcctcgctcg ctagccgcag
     1981 ccgggcgttg agggcttcca tcagccggaa ggcgccgatg tccttcgcca cgtcgacgtg
     2041 cacccgcatc gcccgggcat gaaccccggc atcggtccgc ccagccgcat gcagcgtcac
     2101 cgtctccccg gtgaccgaga gcaccgcttc ctccaccgcc tgctgcacgc tcggcccatg
     2161 cgcctgccgc tgcagcccca tgtagggacc gccatcgaat tcgagggtga gcgcgaagcg
     2221 ggtcacgagc cgaacgcccc tcccgcaggt gggagggcaa gggggtgggc atggcgccac
     2281 acgatcctca tcccagtacc gtccccgccg gaaggctcca cccgcgcaac aggtcgccgg
     2341 ca
//
LOCUS       DATNBV010000474         4103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_904827,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000474 DATNBV010000000
VERSION     DATNBV010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904827"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VN627_11200"
     CDS             <1..428
                     /locus_tag="VN627_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXD03862.1"
                     /translation="TMGAGLPSAMMSAMLYPERKVMAICGDGGFMMNSQEMETAVRLG
                     LNLTVLILNDSAYGMIRWKQANMGFSDFGLTYNNPDFVKYAESYGAIGHRVESAAHLK
                     ELLAHCRDTPGVHLIDCPVDYSENDQILNKDIKVLSKAL"
     gene            432..1868
                     /locus_tag="VN627_11205"
     CDS             432..1868
                     /locus_tag="VN627_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXD03863.1"
                     /translation="MIQLAATYPLYLNNKAAQPNTDLAVTDKFTGEVAFRTALATPDV
                     IDQAIAGAQRAARPMAEMAAYERQAVLDHCVKRFRERFDELAWALCVEAGKPLADSEG
                     EVTRLIDTFRIAAEEATRNYGEIQPLDISPRAKGYVGMWKRVPIGPCSFISPFNFPLN
                     LAAHKIAPAIAVGCPFVMKPASLTPLGAIIMGEVLAETDLPEGAFSILPASRDGADLF
                     TTDERLKLLSFTGSPDVGWALKAKAGKKKVVLELGGNAAVVVDKDADLDHALARIVFG
                     GYYQSGQSCIHVQRVIVHEDIYDTFRAMLAARVKTLVSGDPKDRQTFIGPMISVREAQ
                     RLKGWVDEAVAGGATLLAGGGCDGAMLEATLVENCSRDTALYREEAFGPAVILSRFSN
                     WDEALDEVNDSKFGLQCGLFTRDLHKVMTAWDRCEVGGVVVNDVSSYRVDNMPYGGVK
                     DSGLGREGIRFAMEDMTEIRNLVIRREM"
     gene            complement(1905..2288)
                     /locus_tag="VN627_11210"
     CDS             complement(1905..2288)
                     /locus_tag="VN627_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03864.1"
                     /translation="MLHELRADLELKAAIRPHGPRPFKRGNWMRLLGQVLKLAGGHGE
                     LLRHDERPWASVTFSGTRHTIALAFTGTKAITAAERVIDNLCDHEFDIPGHIVADAQV
                     REVSHQHVPAHQLTMELEILLLEDL"
     gene            complement(2278..2466)
                     /locus_tag="VN627_11215"
     CDS             complement(2278..2466)
                     /locus_tag="VN627_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03865.1"
                     /translation="MLLFKIERFLRDTGMPWTKFGRLATHDPRFVEDLRNGRTPRPAT
                     AQRVEHFMNEYWRNPHAA"
     gene            2563..3228
                     /locus_tag="VN627_11220"
     CDS             2563..3228
                     /locus_tag="VN627_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HXD03866.1"
                     /translation="MINANPRSRLLALATEQGVSLAALSAMIGRNPTYLQQFVRKGSP
                     RKLEEQDRRRLAEFLGVEESDLGAPEYNSSAPVNALAGKRSGKDGWLDIPRLAVEASA
                     GPGAFAGGDEAVGALRFSATWLRQQGFEPGQLSAILVAGDSMEPLLRDGDEILVDRTP
                     RPLRDGVHVVRVGDALMVKRVQTGVPGRIVLESQNPAYRPIELAPEEVEVVGRVVWKS
                     GRL"
     gene            3281..3946
                     /locus_tag="VN627_11225"
     CDS             3281..3946
                     /locus_tag="VN627_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXD03867.1"
                     /translation="MSETQTMPLRAAIVPVTAFEQNCSFLWCTRTMRGVLVDPGGDLP
                     RLKQGIEKAGITVEKILVTHGHMDHCGMAGVLAQELGVPIEGPHEDDRFWIEQLDSSQ
                     SKFGLEGHSFEPDRWLVDGDKVTFGEVELDVIHCPGHTPGHVVFHHAPSHLAVVGDVL
                     FAGSIGRTDFPRGNHQDLIDSITLKLWPLGDDTTFIPGHGPTSTFGEERRSNPFVADQ
                     RFG"
BASE COUNT          718 a         1328 c         1315 g          742 t
ORIGIN      
        1 cgacgatggg cgcagggctg ccctcggcga tgatgagcgc catgctctat cccgagcgca
       61 aggtcatggc gatttgcggc gacggcgggt tcatgatgaa cagccaggag atggagacgg
      121 cggtccgtct cggcctcaac ctcaccgtac tgatcctgaa cgacagcgcc tatggcatga
      181 tccgctggaa gcaggccaac atgggtttct ccgatttcgg gctgacctac aacaaccccg
      241 atttcgtcaa atacgccgaa agctacggcg ccattggcca ccgtgtggaa tcggccgcgc
      301 acctcaagga gttgctggcc cattgccgcg acacgcccgg cgtccacctc atcgattgtc
      361 cggttgacta ttcggagaac gaccagatct tgaacaagga catcaaggtc ctgtcgaagg
      421 cgctctgagc catgatccag cttgccgcga cctacccgct ctatctcaac aacaaggccg
      481 ctcagcccaa caccgacctt gccgtgaccg acaagttcac cggcgaggtg gccttccgca
      541 ccgcgctggc gacgccggac gtgatcgatc aggccatcgc cggggcacag cgcgcggcgc
      601 ggccgatggc cgaaatggcg gcttatgagc ggcaggcagt gctcgatcac tgcgtcaaac
      661 gcttccgcga gcggtttgac gaacttgcct gggcgctctg cgtcgaggcg ggcaagccgc
      721 tggcggattc ggagggcgag gtcacccgtc ttatcgatac cttccgcatc gccgccgaag
      781 aggcgacgcg taattatggc gagatccagc cgctcgacat cagcccgcgt gccaagggct
      841 atgtgggcat gtggaagcgg gtgccgatcg ggccgtgctc gttcatcagc ccgttcaact
      901 tcccgctgaa cctcgccgcc cacaagatcg ccccggccat tgccgtgggc tgccccttcg
      961 tgatgaaacc ggcctcgctc acccccttgg gcgcgatcat catgggcgag gtcctggccg
     1021 agaccgacct gcccgaaggc gccttctcga tcctcccggc cagccgcgac ggggccgacc
     1081 tgttcaccac cgacgagcgg ctcaagctgc tctcgttcac cggctcgccc gacgtgggct
     1141 gggcgctcaa ggccaaggcg ggcaagaaga aggtcgtgct cgaactcggc ggcaatgccg
     1201 ctgtggtggt cgacaaggat gccgaccttg accacgcgct cgcccgcatc gtcttcggcg
     1261 gctactacca gtccggccag tcgtgcatcc acgtccagcg cgtcatcgtg cacgaggaca
     1321 tctacgacac cttccgcgcc atgctcgccg ctagggtcaa aacgctggtc tcgggcgatc
     1381 ccaaggatcg gcagaccttc atcggcccga tgatctcggt gcgcgaagcc cagcgcctga
     1441 agggctgggt ggatgaggcc gtggccggtg gcgccaccct gttggccggc ggcggctgcg
     1501 atggcgccat gctcgaagcc acgctggtgg aaaactgcag ccgcgacacc gcgctctacc
     1561 gcgaggaagc cttcggccct gcggtcatcc tctcgcgctt cagcaattgg gatgaggcac
     1621 tcgacgaggt gaacgacagc aagttcggcc tgcaatgcgg cctgttcacc cgtgacctgc
     1681 acaaggtgat gactgcctgg gaccgctgcg aggtgggcgg cgttgtcgtc aacgacgtgt
     1741 cttcgtatcg ggtcgacaac atgccctacg gcggggtcaa ggattctggc ctcggccgtg
     1801 aaggcatccg cttcgccatg gaagacatga ccgagatccg taatctcgtc attcgccggg
     1861 agatgtaacc ccggccgcag ccgggatcac atccagcaca cgaatcagag gtcctcgagc
     1921 aggagtattt ccagctccat cgtcagctgg tgcgctggca cgtgctggtg gctgacttcg
     1981 cgcacctggg catcggccac gatgtggccg ggaatgtcga actcgtgatc acacaagtta
     2041 tcgattaccc gttcggccgc ggtgatcgcc ttggtaccgg tgaaagccag ggcaatggtg
     2101 tgacgcgtgc ccgagaaggt gacgctggcc catggccgct cgtcatggcg cagtagctcg
     2161 ccgtggccgc cggccagctt cagaacctgg cccagcaggc gcatccagtt gccgcgcttg
     2221 aacgggcgag ggccgtgtgg gcggatcgcg gccttgagtt cgagatcggc gcgcagttca
     2281 tgcagcatgg gggttcctcc agtattcgtt catgaaatgt tccactcgtt gcgcggttgc
     2341 ggggcgaggg gtgcggccat tgcgcaggtc ttccacgaag cgggggtcgt gggtggcaag
     2401 gcgcccgaac ttggtccagg gcatcccggt atcgcgcagg aatcgttcga tcttgaagag
     2461 cagcattggc ttgtcccggc tccgactcgg tggctgtgaa acgattacat cacgggagtt
     2521 ttcctacttg tctaggaaaa atacatcgac tatgtaggaa tcatgattaa cgccaatccc
     2581 cgcagccgcc tgcttgcctt ggccaccgag cagggcgtca gtcttgccgc cctgtcggcc
     2641 atgatcgggc gcaatcccac gtatctacag cagtttgtcc gcaagggtag cccgcgcaag
     2701 ctggaggagc aggaccgtcg tcggctcgcc gaattcctcg gcgtggagga atcggatctc
     2761 ggcgcgccgg agtataattc ctccgcccca gtcaatgcct tggctggtaa acgctccggc
     2821 aaggatggct ggctcgatat tccgcgcctc gcggtcgagg cttcggccgg gcccggcgct
     2881 tttgccggcg gggacgaggc ggtgggcgcc ctgcgcttct cggccacctg gctgcgtcag
     2941 cagggcttcg agccgggcca gctttcggcc atcctcgtcg cgggcgactc gatggagccg
     3001 ctcctgcgcg acggcgacga gatcctggtc gaccgcaccc cgcgtcccct gcgcgatggc
     3061 gtccacgtgg tccgcgtcgg cgatgcgctg atggtcaagc gggtgcagac cggcgtgcct
     3121 gggcggatcg tgctggaaag ccagaatccg gcctatcgcc cgatcgaact ggcaccggaa
     3181 gaggtcgagg tggttggtcg ggtagtgtgg aagagcgggc gcttgtgagc ccacgatgag
     3241 ccattgattc cgcagcccca aggcgccatc tccgctcgcc atgagcgaaa cccaaaccat
     3301 gccgctgcgc gccgcgatcg tgcccgttac cgctttcgag cagaactgtt cgttcctgtg
     3361 gtgcaccaga acaatgcgcg gcgtgctggt cgaccccggc ggggacctgc cgcgtctcaa
     3421 gcagggcatc gaaaaggccg ggatcacggt cgagaagatt ctcgtcaccc atgggcacat
     3481 ggatcactgc ggcatggccg gcgtgctggc gcaggagctt ggcgtgccga tcgaaggtcc
     3541 gcacgaggat gaccgcttct ggatcgaaca gcttgattcg tcccaatcga agttcggcct
     3601 cgaaggccac agtttcgagc cggaccgctg gctggtcgat ggcgacaagg tcacctttgg
     3661 cgaggtcgag ctcgacgtga tccactgccc cgggcatacc cccggacacg tggtcttcca
     3721 ccacgccccc tcgcacttgg cggtggtcgg cgatgtcctg tttgccggtt ccatcggccg
     3781 cacggacttc ccgcgcggca atcaccagga cctgatcgat tcgatcaccc tgaagctgtg
     3841 gccgctcggc gacgatacga ccttcattcc cggtcatggc cccaccagca ccttcggcga
     3901 ggaacggcgc agcaacccct tcgtggccga ccagcgcttc ggctgagcgg tgttcgcccc
     3961 gggacgatca gccgattaca ttacttgaag ggcgatcagc acggccacca cggcaagccc
     4021 cagggtgatc agcatgttga gcagggtccc gaccatgcgg cccttttcta gcgagccgcc
     4081 gtccatgccc agcgcatgtg cca
//
LOCUS       DATNBV010000475         2121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1845891,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000475 DATNBV010000000
VERSION     DATNBV010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845891"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VN627_11230"
     CDS             <1..793
                     /locus_tag="VN627_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD03868.1"
                     /translation="EAGFLSLALEPDLGPVEGALVANRVALRGHPAAVGATALVYPLL
                     TGRAATGPAALAPGKAGVPFRFAPVAAHVLIDGGDEALLLDAGLGDIVRVENDRAGPM
                     LGKLTDAGLARARSLGPGTGAKLRQWWQVALAAEAAGAMRGAIDTTARYLTERVQFGR
                     PLATFQALQHRLAHLAVRIEATYWLAMEAAFRQDSPDFAAVAATQAVATAPLAFSECQ
                     QMHGAMGFTREYPLHVWTMQLPALQRELGGLSAHARAAALLRYAA"
     gene            903..>2121
                     /locus_tag="VN627_11235"
     CDS             903..>2121
                     /locus_tag="VN627_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXD03869.1"
                     /translation="MDASGQQSLLHDGFILLGFALVFVLVFRRLGLGATLGYLLAGAV
                     VGPQVLGLVGDAEQKLGIAELGITLLLFLVGLELNPSRLWRMKRDILGLGLLQVSLCG
                     LALAALIRFTTGFSIEAALALGLPLALSSTAQVLPMLQSSGRLRTPFGERAFSILLFQ
                     DLSIIPLITITAAMSRNPADMGGPPGWLMALETIVAIVGLIAAGRYVIRPLFRLIGNL
                     GEREMFVVAALFTVMASAAVMELLGLSTALGAFIAGVMLADSPYRHELEADVEPFRSI
                     LLGLFFLAVGMMLNLHAIFERPVFVLAMAAALVVVKAAVIMGIGLVFRMTWRGALALG
                     LLLSQGGEFGFVLFAQAANALLVAPEAASLFGAVVTVSMATTPFLMMATRKFREEPAP
                     PLGEREGPSADGAN"
BASE COUNT          251 a          752 c          743 g          375 t
ORIGIN      
        1 ggaggcaggc ttcctctcgc tggcgctgga gccggacctt ggcccggtcg agggtgcgct
       61 ggtcgccaac cgcgtggccc tgcgcggcca ccctgccgcc gtcggcgcca ccgcgctggt
      121 gtacccgctg ctgaccgggc gggctgcgac cggccccgcc gcgctggcgc cgggcaaggc
      181 cggcgtgccc ttccgtttcg ccccggtcgc cgcacacgtg ctgatcgacg gcggggatga
      241 agcgctgttg ctggacgccg ggctgggcga catcgtgcgg gtcgagaacg atcgcgccgg
      301 cccgatgctg ggcaagctga ccgacgcggg cctcgcccgc gcccggtcgc tcggccccgg
      361 cacgggcgcg aagctgcgtc agtggtggca agtggcactg gccgccgaag cggcaggagc
      421 gatgcgcggg gcgatcgaca ccaccgcccg ctacctgacc gagcgggtgc agttcggccg
      481 cccgctggca acgttccagg ccttgcagca ccgtctggcc catctcgcgg tgcggataga
      541 ggcgacctac tggctggcga tggaggcggc cttccggcag gattcgccgg atttcgcggc
      601 ggtggcggcg acccaggcgg tggcgaccgc gccgctggcc ttcagcgaat gccagcagat
      661 gcacggcgcg atgggcttca cccgggagta tccgctacac gtctggacca tgcagctccc
      721 cgcgttgcag cgcgaactcg gcgggctttc ggcccacgcg agggcggctg ccctgctgcg
      781 ctacgccgcc tgacggggca agccagcctt cgcgccgggc aagggagacg cggcttgccc
      841 ctgcccgcct gagccggtag cccatacccc tgttccaccg ctgcaccggg ggttttcacg
      901 caatggatgc ttcgggccag cagtccctgc tgcacgacgg tttcatcctg ctcggtttcg
      961 cgctggtctt cgtgctggtc ttccgccggc tcgggcttgg cgcgaccttg ggctaccttc
     1021 tcgccggcgc ggtcgtcggg ccgcaggtgc tgggcctcgt cggcgatgcc gaacagaagc
     1081 tcgggatcgc ggagctcggc atcaccctgc tgctgttcct cgtcgggctg gaactgaacc
     1141 cgtcgcggct gtggcgcatg aaacgcgaca ttctggggct tggcctgctc caggtgtcgc
     1201 tttgcggtct cgccctcgcc gcgctgatcc ggtttaccac cgggttttcc atcgaagccg
     1261 cactcgcgct gggtctgccg ctcgcgctgt cgtcgaccgc gcaggtcctg ccgatgctgc
     1321 aatcgtccgg gcggctgcga acccccttcg gggagcgtgc attctcgatc ctgctgttcc
     1381 aggacctttc gatcatcccg ctgatcacca tcaccgcggc aatgagccgc aatccggccg
     1441 acatgggcgg cccgccgggc tggctgatgg cgctggaaac gatcgtcgcg atcgtcggcc
     1501 tgatcgccgc ggggcgctat gtcatccgcc cgctgttccg cctgatcggc aatctcggcg
     1561 aacgcgagat gttcgtcgtg gccgcgctct tcaccgtcat ggcctccgcc gcggtcatgg
     1621 aactgctcgg cctgtcgacc gcgctcggcg cgttcatcgc cggggtgatg ctggccgatt
     1681 ccccctatcg ccacgagctg gaggccgacg tcgagccgtt ccgctcgatc ctgctcggcc
     1741 tgttcttcct tgccgtgggg atgatgctga acctccacgc gattttcgag aggccggtgt
     1801 tcgtcctcgc catggcagcg gcgctggtgg tcgtgaaggc cgcggtgatc atgggcatcg
     1861 ggctcgtctt ccggatgacc tggcgcgggg cgctggcgct cggcctgctg ctgagccagg
     1921 gcggcgaatt cggcttcgtc ctcttcgcgc aggccgcgaa tgccctgctc gtcgcacccg
     1981 aggccgccag cctgttcggc gcggtcgtca ccgtgtcaat ggcgaccacg cccttcctga
     2041 tgatggcgac ccgcaagttc cgcgaggaac ccgcgccccc gctgggcgag cgcgaggggc
     2101 cgagcgccga cggggcgaat g
//
LOCUS       DATNBV010000476         4987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1554608,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000476 DATNBV010000000
VERSION     DATNBV010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554608"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="VN627_11240"
     CDS             <1..1369
                     /locus_tag="VN627_11240"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD03870.1"
                     /translation="LPWNWESFPDYLDVLDSRSLDIDITVQVPHSALRVYVMGERGAN
                     RDKATPQDLAEMTRLVAEAVRAGAIGVSTSRLLSHRSAGGEMIPSLQSARDELLALGE
                     GLRQAGGGVFQLVPDYDAEPEDEVEIMRDIAAHSGRPLSFSLTQSPQNPAHWERYVKA
                     VANANAAGLDIKGQVFPRPIGFMYGLDLSFNPLCRRPSWAQIADLPLAGRVAALCDPE
                     FRARILAEEDPEHPMPQVNMMLGRLERMVALGDPPNYMPGPEQQLGARAVRAGKDLME
                     FALDAMLEQDGQAVLYLPAGNYCAGDLGAARALMDNEHTVLGLGDGGAHYGIVCDASF
                     PTTMLAYWGRDAAPAERMPIERVVAALTSEPAALIGLGDRGIIAPGKLADINVIERES
                     VLLHAPRVKYDLPGGGRRLTQAADGYVVTIKRGQVTYRNGEPTGALPGSLVRREGAIP
                     VLEAV"
     gene            1626..1883
                     /locus_tag="VN627_11245"
     CDS             1626..1883
                     /locus_tag="VN627_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03871.1"
                     /translation="MTTHAGADQPVTGPSSASARSKTRALESLADMSAGRGYGSPAKI
                     KDLGRNCLCCFGMEVDDCDRSSLGGAKRDCLAYPARHLSQR"
     gene            complement(1929..3374)
                     /locus_tag="VN627_11250"
     CDS             complement(1929..3374)
                     /locus_tag="VN627_11250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD03872.1"
                     /translation="MPQATATPALAEPMDEGRPAAWKVIGILIVAEICATSALAMPIP
                     ALSAFLHLYGSPIAAGWIMSSFLLVSAAVAALCGSLGDRFGRKPVMMAILALVFVGSV
                     MSAPTESFGWVVAGRCLQGAAGATIPLSLGLVREVLPPHRVSFGFGIILASSSAAAAL
                     GFPISGYMTDNYGPSSIFWFVALFAAVALVGINLVLPRPVRPDPSQQAIDWLGGILFP
                     PAIALLLLAISGGRQAGWLADLAWPMAIAGLLLLAFWFRYESRHPAPLINVRLLSNRY
                     CAVGLVVTALVATGAFQIAETVSLLLQQPIWTGVGLGMTAMVVGLLKLPTTGAGALSS
                     LATGWLAGRWGPIPCTLAGASIVAASSFAVQPFLDSLAVVAAALLTISIGLTAALTSA
                     PVLLALGTPHDRTSEVMGVMAVVRAVFQGVGAQVVAVILASWVVIGPNDVHYPAATAY
                     RMAFVYIGVTAILMVLAVTPLLRRGRLRQVQ"
     gene            complement(4014..>4987)
                     /locus_tag="VN627_11255"
     CDS             complement(4014..>4987)
                     /locus_tag="VN627_11255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD03873.1"
                     /translation="TLADRHSRKTLLAGCIAVWSGMTVLCGFATGFLSLLFYRMLVGM
                     AEAGLQPTALSMMADEAHESQRPKAVAIVHIGIPIGTLLGFLLGGWVATNLGWQQALL
                     LVGAPGILLAAVVLLTLREPVRQRTSSGEAGKDVPTSEFFRLLWRSKPLLHVVVGLVL
                     LWLCTSSTSAWMPSFFIRMYGVDLTTVGLIMSLSAGGAGLVGNTLSGYLAQKVGGDRK
                     DRLMLLAAGGAFCYFPLALVTLLSSSLILSVVTHFTQTIFYFMVFTPGYALAMDLADV
                     RIRARTAALVSMGATAIGYGLGPQIVGFGSDLLRPIFDAQSLRWALC"
BASE COUNT          981 a         1644 c         1605 g          757 t
ORIGIN      
        1 gctgccgtgg aactgggaga gctttcccga ctacctcgac gtgctcgatt cccgatcgct
       61 ggacatcgac atcacggtgc aggtgcccca ctccgccctg cgcgtctacg tcatgggcga
      121 acgcggcgcc aatcgcgaca aggcgacgcc gcaggatctc gccgagatga cccggctcgt
      181 cgccgaggcg gtgcgcgccg gcgccatcgg ggtttccacc tcgcgccttt tgtcgcatcg
      241 cagcgccggg ggcgagatga tcccaagcct gcaaagcgcg cgcgacgagt tgctcgcgct
      301 cggcgaagga ctgcgccagg cgggaggcgg cgtgttccag ctggtccccg actacgacgc
      361 cgagccagag gatgaggtcg agatcatgcg cgacatcgcc gcgcattcgg gtcggccgct
      421 gtccttctcg ctgacccaat cgcctcagaa cccggcacac tgggagcgct atgtaaaggc
      481 ggtggcaaac gctaatgcgg ccggtctcga catcaagggg caggtgttcc ctcgccccat
      541 cggcttcatg tatggcctcg atctctcgtt caacccgctg tgccggcgac cgagctgggc
      601 ccagatcgcc gatctgccgc tggccggacg cgtcgctgcc ttgtgcgatc ccgaattccg
      661 cgcgcgcatc cttgccgagg aggatcccga gcatccgatg ccccaggtca acatgatgct
      721 tggtcgcctc gagcggatgg tcgcgctggg agatcccccc aactacatgc caggcccgga
      781 gcagcagctc ggagcgcgcg ccgtcagggc cggcaaggac ctaatggaat tcgcgctcga
      841 tgccatgctc gagcaggacg gccaggcggt gctctacctg ccggcgggca actattgtgc
      901 cggcgacctc ggcgcggcgc gggcgctgat ggacaacgaa cacaccgtgc tcgggcttgg
      961 cgacggcggg gcgcactatg gcatcgtctg cgatgccagc ttccccacga ccatgctggc
     1021 ttactggggc cgcgatgccg ccccggcaga gcggatgccg atagagcggg tggttgcggc
     1081 cctgaccagc gaacccgcgg cgctgatcgg cctcggagat cgcggcatta tcgcgcccgg
     1141 caagctggcc gacatcaacg tgatcgagcg cgaatcggtc ctcctgcacg cgccgcgggt
     1201 gaaatacgac cttcctggcg gcgggcggcg gcttacgcag gcggcggatg gctatgtcgt
     1261 gacgatcaag cgcggtcaag taacctaccg caatggcgag ccgaccggcg cgctgccggg
     1321 ctcgctggtc cgtcgcgagg gggcgatccc cgtgctcgag gcggtgtaat agccgcccgc
     1381 gcggttgtca tcgaggcagc catagcggat cgggtgcgcg agccaatccc aacatcccac
     1441 gaaacatagg cggcgccatt gacgatcgcg ctgcgcgccg cggtcgaagt cacgaacacg
     1501 atcgagccgt ggccacgcgg cagcatgcgg cggccggcct cgcgcgcgct gcggaaataa
     1561 ccggtcaggc catcgccgat gacgacgccc cactgctcgt cggtcattag ctcggccggc
     1621 gcatcatgac gacgcatgcc ggcgcagacc agcccgtgac tgggccaagc tcggcttccg
     1681 cccggtcgaa gacgcgcgcg ttggagtcgc tggccgacat gtcggcgggg cgaggctacg
     1741 gctcgcccgc taagatcaag gatttggggc gcaactgcct gtgctgcttc ggcatggagg
     1801 tcgacgactg cgaccgcagc tccctcggcg gcgccaagcg cgactgcctt gcctatcctg
     1861 cccgccacct gtcacaacga tgacctggct cggaagcatg ccctccattc cggtctgccc
     1921 cttttcggtc attgcacttg acgcaggcgg ccgcggcgta acagcggggt cacggccagg
     1981 accatgagga tcgccgtcac gccgatgtaa acgaaggcca tgcggtacgc cgtggcggcc
     2041 ggatagtgca cgtcattggg accgatcacc acccaactcg ccaggatgac tgcaacaacc
     2101 tgcgccccca ctccctgaaa aaccgcgcgg accaccgcca tcacgcccat cacctcgctg
     2161 gtccggtcgt gtggcgtccc cagtgcgaga agcaccggag cggacgttaa agccgcggtc
     2221 agaccgatac tgatcgtcag aagtgccgcg gcgacgacgg ccagcgagtc aaggaagggc
     2281 tgcaccgcga aactggacgc agccactatc gaagcacccg ccagtgtgca aggaatgggc
     2341 ccccagcgtc cggcaagcca tcccgtggca agcgaggaca gtgcgccagc gccggtcgtg
     2401 ggtagcttga gcagccccac caccatcgcg gtcatgccga gcccgacgcc cgtccagatc
     2461 ggctgctgca gcagcagcga caccgtttcg gcaatctgga atgcccctgt cgcgacgagg
     2521 gccgtcacca ccagcccgac cgcgcagtaa cggttggaca gcagacgcac gttgatgaga
     2581 ggcgcaggat ggcggctttc gtacctgaac cagaatgcga gcaggaggag gcccgcaatt
     2641 gccatcggcc acgccaggtc ggccagccat ccggcctgtc ggcctccgct gatggcaagc
     2701 aggaggaggg cgattgctgg cgggaacaga atcccgccga gccagtctat ggcctgctgg
     2761 gaaggatcag gccgcacagg ccgtggcagg accaggttga tgccgaccag tgcgactgcc
     2821 gcgaacaggg cgacaaacca gaagatgctc gatggaccgt agttgtcggt catgtagccc
     2881 gaaattggaa agccgagggc cgcagcggcg gaggagctgg cgaggatgat cccgaacccg
     2941 aacgatacgc gatgcggcgg caagacttcg cgaaccagtc ccagactcag cgggatggtt
     3001 gccccggctg ccccttgaag gcagcggcca gcaacgaccc agccaaaaga ctcggtagga
     3061 gcgctcatca cgctgccgac gaacaccaag gccaggatcg ccatcatcac cggcttgcgg
     3121 ccaaagcggt cgccgaggct gccgcacaat gctgccaccg cggcagacac cagcaagaac
     3181 gacgacatga tccaccccgc ggcaatcggg ctgccataca agtgcaaaaa cgccgacaga
     3241 gcggggatcg gcatcgccag cgcggaggtg gcgcagattt ccgccacgat caggatccct
     3301 atcaccttcc atgccgcggg ccgcccctcg tccattggtt cggcgagcgc tggcgtcgcg
     3361 gttgcttgag gcattcctct ctcctggctg agtcggctgg cggacttccg ccaagtcatc
     3421 tgctttgccg ctcgtttgat ttacctgttt ttcctctgcc gacaagcaga atgggctccc
     3481 ggagggtgtt ttcccggtat aggacaacct cattgcgctg cgtgccatgt agggcggtac
     3541 gctggaagtc acagccctga tcccgacaag ttcgagcgag gcacgcgctg gcaatcagtg
     3601 gatgcctagc ctcgcgcagc ccccctcgct gcaggcggcg gcgcacgccg gcgcgccctg
     3661 cccgcccgac gtcaaggcag cgatgatcgc gtggtgggga ccgatcatcc atgaattcta
     3721 cttgggcagc gagggcgcga gactgaccca cgatcaggtc ggaggaatgg ctgacgcacc
     3781 cgggctcgtt gggcggcgcg gtgaccggcg caatccgcat cttcgacgag gagggcgaga
     3841 agctgccttc cagacaggtc ggaatcatct gctgggtggc ggacctcgca ggggggcgcg
     3901 cctctaagca tgtggcgcag gattccgatg cggctctcct gccgaagact cattggacag
     3961 atgacgccag gccaatatga agtggctgcc acaccacagg acggtcagca tcatcaacac
     4021 aaggcccagc gcaacgactg tgcgtcaaag atcggtcgca acaaatcgct gccgaaaccc
     4081 acgatttgcg gcccgaggcc gtagcctatc gcggtagcgc ccatggagac cagagcggcg
     4141 gtacgagccc ggatgcgaac atcggcgagg tccatggcca acgcatatcc aggcgtgaac
     4201 accatgaaat agaagatcgt ctgcgtgaaa tgcgtaacta ccgacaaaat caaacttgac
     4261 gaaagtaatg taaccagcgc caatgggaag tagcagaagg ctcccccggc ggcgagcagc
     4321 atcaggcgat ccttgcggtc gcccccgacc ttctgtgcga gatacccgct caatgtattc
     4381 cccacgaggc ccgccccccc tgccgaaagc gacatgatga ggccgaccgt ggtcagatcc
     4441 acaccgtaca tgcggatgaa gaaggagggc atccacgcgg atgtgctgga ggtgcacaac
     4501 cataacagca cgagcccgac aacgacgtgc aacaacggct tcgagcgcca cagcaagcga
     4561 aaaaattcgc tggtcggcac atccttgccg gcttcgcctg aggaggtgcg ttggcggacc
     4621 ggctcccgca aggtcagcag gacgaccgcg gcgagcaaaa tccccggggc acccaccagg
     4681 agcagggctt gctgccaacc cagattggtc gcgacccatc cgccgagaag gaagccgagc
     4741 agggttccga tcggtatgcc aatatgaacg attgcgaccg ctttgggacg ctggctttcg
     4801 tgggcttcgt ctgccatcat cgacagcgcg gtcggttgaa gcccggcctc cgccatcccg
     4861 acaagcattc ggtagaacag cagggacaga aagccggtcg caaaaccgca cagcaccgtc
     4921 attccgctcc aaacggcaat gcatccggca agcaacgtct tgcgcgaatg gcgatcggca
     4981 agggtcc
//
LOCUS       DATNBV010000477         3466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1556938,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000477 DATNBV010000000
VERSION     DATNBV010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556938"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /gene="phaZ"
                     /locus_tag="VN627_11260"
     CDS             complement(<1..1165)
                     /gene="phaZ"
                     /locus_tag="VN627_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HXD03874.1"
                     /translation="MLYNAYELNRAWLTGASAWASVTAELLNNPALPLGYNGLGPILA
                     SALDVFAHAAAPRGKPEFGIGTATVNGTTYPVTEAIVVRRPFGNLLRFTHPGLPANAP
                     RLLIVAPMSGHFATLLRGTVARLVEGMEVFITDWADAKLVPAEEGTFDVDDYIDYIVG
                     FLEHIGPGAHMLAVCQPSVPALAATAVMGAARHPCRPASLTMMGGPVDTREAPTSVND
                     VAVTRPLSWFKHNVIATVPLGYPGAGRKVYPGFLQLAGFMSMNLRSHLQSHYQIFQHL
                     VEGDGESADGKKAFYDEYRSVCDMTAEFYLQTIEHVFQRHSLPKGEFVHHGKPIDLGA
                     ITDTAILAVEGERDDISGIGQTRAALKLTTGLAEDRKLYHLAPEVGHYGIFNGT"
     gene            1356..3176
                     /locus_tag="VN627_11265"
     CDS             1356..3176
                     /locus_tag="VN627_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HXD03875.1"
                     /translation="MARQPSAAAAAAAAPDEAKPARNLGPLAMIWQLGSRYPKQVGIA
                     LLALMTTSSATIAIPMRFKMIVDKAFGSGARADDIAHAFEGLIAIVLVLGLATAVRFY
                     FVSWLGERVVADLREKVQANLLRLPPSFFEANSPKEISSRMTSDTALIEVVVGTTVSI
                     ALRNAITGIGGTIYLFFLAPRLTLELLVVIPLVIVPIVTFGRRLKGASRISQDRIADV
                     GAKVAEVLGAMRVVQSFNQEGREAENFAVVVEQTFAAAKRRILIRSIMTAVVMVLVFG
                     SITVLLWQGASAVAAGTISGGTIAAFVVTAGLVAGAFGALTEVYGELLRGAGAAGRLN
                     ELLNEKPAIAPPARPLELPNPPRGRLTFESVTFRYPARPELPAIQQFSLSVEPGETVA
                     IVGPSGAGKSTVFQLAQRFYDPQTGTIKLDGVPLVSADPAEVRRRIALVPQEGVLFAA
                     SARDNLRYGNWDASDEAIWDAARAANAEEFLRELPEGLDTFLGENGSRLSGGQRQRIA
                     IARALLRDAPILLLDEATSALDAESERLVQDALDRLMASRTTLVIAHRLATVRQADRI
                     VVMDGGRIVEEGPHDQLAAAGGLYARLASLQFDDRAMAAG"
     gene            3250..>3466
                     /locus_tag="VN627_11270"
     CDS             3250..>3466
                     /locus_tag="VN627_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03876.1"
                     /translation="MTLPRLPLPRLPLFAASLLALSAVPALAGPPATAPNPEEVLTGW
                     GRSGVQQQWNDPAIRPGDDFFRFVNGKW"
BASE COUNT          547 a         1135 c         1172 g          612 t
ORIGIN      
        1 gggtgccgtt gaagatgccg tagtgcccga cttcgggagc aaggtggtag agcttgcggt
       61 cctcggccag gccggtggtc agcttgagcg cggcgcgggt ctgcccgatg cccgagatat
      121 cgtcgcgctc tccttcgacc gcgagaatgg cggtatcggt gatcgcgcca aggtcgatcg
      181 gcttgccgtg gtggacgaac tcgcccttgg gcagcgagtg gcgctggaac acgtgctcga
      241 tcgtctggag gtagaattcc gcggtcatgt cgcagactga gcggtactcg tcgtagaacg
      301 ccttcttgcc atcggcgctt tcgccgtcac cctccaccag gtgctggaag atctggtagt
      361 ggctctgcag gtgggaccgc aggttcatcg acatgaaccc ggccaattgg agaaagccgg
      421 ggtaaacctt ccggcccgcg cccgggtagc cgagcggcac cgtcgcgatg acgttgtgct
      481 tgaaccacga cagcggccgg gtcacggcga cgtcgttgac gctggtcggc gcctcgcggg
      541 tgtcgaccgg gccgcccatc atggtcagcg aggccggccg gcagggatgc ctggccgcgc
      601 ccatgaccgc cgtggctgcc agtgccggga ccgagggctg gcacactgcg agcatgtggg
      661 cgcccgggcc gatgtgttcg aggaagccga cgatgtagtc gatgtagtcg tctacatcga
      721 aggtgccttc ctcggcggga accagcttgg catcggccca gtcggtgatg aacacttcca
      781 tgccttcgac cagccgggca accgttccgc gcagcagcgt ggcgaagtgg ccgctcatgg
      841 gcgcgacaat cagcaggcgc ggcgcattgg cgggcaggcc cgggtgggtg aagcgcagca
      901 ggttgccgaa gggccggcgc acgacgatcg cttcggtgac cgggtaggtc gtgccgttca
      961 ccgtggccgt gccgatcccg aactccggct tgccgcgcgg ggccgccgca tgggcgaaga
     1021 catccagtgc cgaggcgagg atcgggccaa gcccgttata gcccagcggc agggcggggt
     1081 tgttgagcag ctcggcagtg accgaggccc atgcgctggc cccggtcagc caggcccgat
     1141 tcagttcgta ggcattgtac agcaaggctc gcgatcctta tctgcggtgc gcggaagcaa
     1201 ccggttgcga tcaagatgct acgatgcaga cgtttttgca atgcagcatc gataataacg
     1261 agtgtccgag cggaattccc cgaaagctga ttaattctgc gcaatgcgca atgcggtgct
     1321 tccgccgcag gcccgcctgc gctagggccc gcgcgatggc tcgccaacct tccgctgccg
     1381 ctgctgccgc cgccgcgccc gacgaggcaa agccggcgcg caatctcggc ccgcttgcca
     1441 tgatctggca gctgggatcg cgctatccca aacaggtggg catcgccctg cttgcgctga
     1501 tgactacgtc gtcggccacg atcgccatcc cgatgcgttt caagatgatc gtcgacaagg
     1561 cgttcggcag cggtgcccgg gccgacgata tcgcccacgc cttcgagggc ctgatagcca
     1621 tcgtcctggt gctgggtctg gccacggcgg tgcggttcta cttcgtttcc tggctgggcg
     1681 aacgtgtcgt cgccgacctg cgtgagaagg tccaggccaa tctcctgcgc cttcccccca
     1741 gcttcttcga ggcgaacagc cccaaggaaa tctcctcgcg catgaccagc gataccgcgc
     1801 tgatcgaggt tgttgtgggg acgaccgtgt cgattgccct gcgcaacgcg atcaccggaa
     1861 tcggcggaac catctacctt ttcttcctgg ctccgcggct gacgctggag ctgctggtgg
     1921 tgatcccgct ggtgatcgtg cccatcgtca ccttcgggcg gcgactgaag ggtgcttcgc
     1981 ggatcagtca ggaccgaatc gccgatgtcg gcgccaaggt cgccgaggtg ctcggcgcga
     2041 tgcgggtggt ccagtcgttc aaccaggaag ggcgcgaggc tgaaaatttc gcggttgtgg
     2101 tggagcaaac cttcgccgcc gcgaagcgcc gcattctgat tcgctcaatc atgaccgcgg
     2161 tggtcatggt cctcgtcttc ggctcgatca ccgtgctgct gtggcagggg gcgagcgccg
     2221 tggccgccgg gacgatctcg gggggaacca tcgccgcatt cgtcgtcacc gcagggctgg
     2281 tcgccggagc cttcggggcg ctgaccgaag tatatggcga gctgctgcgc ggcgcgggag
     2341 cggcgggccg gctcaacgaa ttgctcaatg aaaagccggc gatcgccccg ccggcgcgcc
     2401 cgctcgaact gccaaacccg ccgcgcgggc ggctgacctt cgagagcgtg accttccgct
     2461 atccggcacg gcccgagctg ccggcgatcc agcaattctc gctcagcgtg gagccgggcg
     2521 aaaccgtggc cattgtcggc ccttcgggcg ccggtaaatc caccgtgttc cagctggccc
     2581 agcgattcta cgatccgcaa acgggcacga tcaagctcga cggggtgccg ctggtctctg
     2641 ccgatcccgc cgaggtccgc cgccggatcg cgctcgtgcc ccaggagggg gtactgttcg
     2701 ccgcctccgc gcgcgacaat ctgcgctacg gcaactggga cgcttcggac gaagcaatct
     2761 gggatgcggc ccgcgcggcc aatgccgagg aattcctgcg tgaattgccc gaggggctag
     2821 atactttcct cggtgagaac ggctcgcgcc tgtcgggcgg gcagcgccag cgaatcgcca
     2881 ttgcccgcgc tctccttcgc gatgccccga tcctgctgct cgacgaggcg acctccgcgc
     2941 tagacgcgga aagcgaacgc ttggttcagg acgcacttga tcggttgatg gccagccgca
     3001 cgaccctcgt catcgcccat cgcctcgcca cggtgcgcca ggccgaccgg atcgtggtga
     3061 tggacggcgg gcggatcgtc gaggagggcc cgcatgacca gctggccgct gccggcgggc
     3121 tctacgcccg cctcgcctcg ctccagttcg atgaccgcgc catggcggca gggtgaaaga
     3181 ctgcaatcgg cggttaagct caatgcggca tatctcgggc cacgccgtct tctgccaagg
     3241 aacaatccct tgaccctgcc ccgcctgccg ctgccccgcc tgccgctttt cgccgcttcc
     3301 ctcctcgcct tgtccgcagt accggcactg gccgggccgc cggcaaccgc gccgaacccg
     3361 gaagaggtgt tgaccggctg gggccgttcg ggcgtgcagc agcagtggaa tgaccccgct
     3421 atcaggccgg gcgacgattt cttccgcttc gtcaacggca agtgga
//
LOCUS       DATNBV010000478         3518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_286473,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000478 DATNBV010000000
VERSION     DATNBV010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286473"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VN627_11275"
     CDS             complement(<1..617)
                     /locus_tag="VN627_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXD03877.1"
                     /translation="MAGKDMVVLSDYKDKYETARLERRDGILQIQLHTRGKEYCNSAT
                     AHTELVDLFSNVAADHGNRVVILTGTGNAFGNQIEGSAFGGDGDDVARVHYEGRKIME
                     NVLAIEAPMIAAINGPMTTLPPFALTCDITLCSDNATFSDIVHFPANNVVPGDGVQTV
                     WEELLGVNRARYFLLMGQELDAQAAFNLGVVNEVLPHEQLQARAW"
     gene            993..1526
                     /locus_tag="VN627_11280"
     CDS             993..1526
                     /locus_tag="VN627_11280"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXD03878.1"
                     /translation="MLRLVKLVALALFGLVMVPETAGAVVLDISIFGDVTGTNTRAAC
                     EPGQTFPFCPVETLPYTATLAQDLGPIDLMPGDNAFSYGGYYSTGLITGTINYDNGVL
                     TGRDLLYTYAGCSGPCAGDHIVASAATFVVTGGVPEADTWAMLLVGIGAVGWVLRRRR
                     GAGALGAFSILPVERRA"
     gene            complement(1618..2334)
                     /locus_tag="VN627_11285"
     CDS             complement(1618..2334)
                     /locus_tag="VN627_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXD03879.1"
                     /translation="MDQYEDEAERHPFDPPAEADREHEIHEGAAPKLALDDDDRLPWL
                     ESADDDDDDEGAIDTGRVLRFLVFGLVLLAAIGGALWFVKHRAATAPTVAEGGVIKAP
                     AEPYKAVPANQGGKTYAGTGDSAFAASEGKTTQARLGGGEAPVPAPSVDLANGAAPAP
                     VGVGVQVGAYTSQAAAEAGWTRLSGSSEALKAVKHRVVQGSADIGTVYRLQAVAPDAA
                     AANALCSQLKAAGIACQVKG"
     gene            complement(2367..>3518)
                     /locus_tag="VN627_11290"
     CDS             complement(2367..>3518)
                     /locus_tag="VN627_11290"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXD03880.1"
                     /translation="EIPEGLYPGDYLVPVGQDLAAEFGDRYAKAPEAEWLALFRTRAV
                     AAMMEMIRADLALLGITHEVFASEAELQASGKVDAAEAWLRAHDLVYDGLLEAPKGKT
                     PEDWEPVELPLFRSTKFGDDQDRPIKKSDGYWTYFGADLAYHFQKAQECDALVDIWGA
                     DHAGTVKRIKAAVAALTGADGHKPKPFEVKLVQMVQLLRDGEPVKMSKRAGTFVTLAD
                     VVREVGTDVVRFTMLTRKPEAQMDFDFAKVVEASKDNPVFYVQYAHARICSTMRKAAA
                     EGFAPSPEGLDRLGEEELALVKLASQFPRLVEAAAAAREPHRVAFFLGDLAAAFHAYW
                     NIGNDRPDKRFIVTSDAVLTGARLFLGQQIGQLIRNGLAILGVEAVEEL"
BASE COUNT          570 a         1046 c         1224 g          678 t
ORIGIN      
        1 tcccacgcgc gcgcctggag ctgctcgtgc ggaagcactt cgttgactac gccgaggttg
       61 aacgcagcct gggcatcaag ctcctgtccc atcagcagaa aataccgtgc gcgattcacg
      121 cccagcagtt cttcccaaac agtctgaacg ccatcaccgg gcacgacgtt gttcgccggg
      181 aagtgcacga tatcggaaaa ggtcgcattg tccgagcaga gcgtaatatc gcaggtcagg
      241 gcaaagggtg gaagcgtggt catcggcccg ttgatggctg cgatcatcgg agcttcgata
      301 gccaggacat tttccatgat cttgcgtcct tcgtagtgga cgcgcgcgac atcgtcgcca
      361 tctccgccga aggctgaacc ttcaatctga ttgccgaatg catttccggt gccagtcaag
      421 atcacgacgc ggttgccatg atcggcagcg acattgctga acagatcgac gagctctgtg
      481 tgggctgtcg cactgttaca atattctttc ccgcgtgtat gcaactgtat ctggagaata
      541 ccgtcgcgcc gctcaaggcg ggcagtctcg tatttgtcct tgtagtcaga caggactacc
      601 atgtccttcc cggccatacc tgatctccca acatcgtctt atattcgagg gtgattcctg
      661 atagagcagg cataaacgcg tggaaagccg aaactagtct agggttggca gcgggcggat
      721 ggtgcaatcg gcggaaagcc aatcgggccc agtcctccgg tccgcgatgg tggcgtggct
      781 gcaatggcac gaaccgtggc aatttagccc gtagctgccg gtaaaattac acaccgccgt
      841 gcgtgactga tcattttctc caattgcgac aaacggttgg aaccgggtcg tcggacgcaa
      901 cgttggtttt gctgacgggg attccttcgt cctttgccca tgcggggcct ggggtggcgg
      961 catcggaccg atagcacagg ggacatcggc tgatgctgcg cttggtgaaa ctggtcgctc
     1021 ttgctttgtt tgggctggtc atggtcccgg aaaccgcggg cgcggtggtt ttggatatca
     1081 gcatcttcgg cgatgtgacg ggcacgaaca cgcgggcggc ctgcgagccg gggcagacct
     1141 tccctttttg cccagtggaa acgctgcctt acacggccac tttggcccag gatctcggac
     1201 ccatcgacct catgccgggg gacaatgcgt tcagctacgg cggctattat tcaaccgggc
     1261 tgatcaccgg gacgatcaac tatgacaacg gcgtcctgac cgggcgcgac ctgctgtata
     1321 cctatgcggg ctgttcgggg ccctgcgcgg gagatcacat cgtggccagt gcggcgacgt
     1381 tcgtggtgac cggcggggtg ccggaggctg acacctgggc catgttgctg gtggggatcg
     1441 gcgcggtggg ttgggtgttg cggcggcggc gcggggcagg ggcgctgggc gccttttcaa
     1501 tcctgccggt cgaacgccgc gcctgagcgc gggttcgggt attccctgcc aagttgggat
     1561 aggcccctgc ctgcgcaggg gcgacggtta ttcggcggcg gttgtgggcc ggcccgttca
     1621 gcccttgacc tggcaggcga tgccggcggc cttgagctgg ctgcacaggg cattggcggc
     1681 ggcggcatcc ggggcgacgg cctggagacg gtagaccgtg ccgatatcgg ccgagccttg
     1741 cacgacacgg tgtttcacgg ctttcagggc ttcgctgctg ccagagaggc gggtccaccc
     1801 cgcttcggcg gcggcttgcg aggtgtaggc cccgacctgg acgccgacgc cgaccggcgc
     1861 gggtgcggcg ccgttggcga ggtcgaccga gggagcgggg accggggctt cgccgccgcc
     1921 gaggcgggcc tgggtggtct tcccttccga ggcggcaaag gcgctatccc cggtgccggc
     1981 gtaggtcttg ccgccctggt tggcggggac ggccttgtag ggttcggccg gggccttgat
     2041 cacgccgcct tcggcgacgg tgggggcagt ggcggcgcgg tgcttgacga accacagggc
     2101 gccgccgatg gcggcaagca ggacgaggcc gaagacgagg aagcgcagga cgcggcccgt
     2161 gtcgatcgcg ccttcatcgt catcatcatc gtcagcggat tcgagccaag gcaggcgatc
     2221 gtcatcatcc agcgccagct tgggagcggc gccttcatgg atttcgtgtt cgcggtccgc
     2281 ctctgcgggg ggatcgaacg ggtggcgttc ggcctcatcc tcgtattgat ccatcatgcc
     2341 ctccgttggc cgtgctggcc ccgcgttcaa agttcctcaa ccgcctcgac cccgagaatg
     2401 gcgaggccgt tcctgatcaa ttgcccgatt tgctgcccga ggaaaagcct tgccccggtg
     2461 agaaccgcat ccgatgtgac gatgaagcgc ttgtccggcc ggtcattgcc gatgttccag
     2521 taggcgtgga aggcggcggc gagatcgccg aggaagaagg cgacgcggtg cggttcgcgc
     2581 gcggcggcgg cggcttcgac caggcggggg aattgagagg cgagcttgac cagggccagt
     2641 tcttcctcgc ccagccggtc caatccctcg ggcgaggggg cgaagccctc tgcggcggcc
     2701 ttgcgcatgg tcgagcagat gcgcgcgtgg gcatactgca cgtagaacac cgggttgtcc
     2761 ttcgaggctt cgaccacctt ggcgaagtcg aagtccatct gggcttcggg cttgcgggtg
     2821 agcatggtga agcggacgac gtccgtgccg acttcgcgca cgacatcggc aagggtgacg
     2881 aaggtgccgg cgcgcttgga catctttacc ggttcgccgt cgcgcagcag ctggaccatc
     2941 tggacgagct tgacctcgaa gggcttcggc ttgtggccat ccgcgccggt gagcgccgcg
     3001 acggcggcct tgatccgctt gaccgtgccg gcatgatcgg cgccccagat atcgacgagc
     3061 gcgtcgcact cctgcgcctt ctggaagtgg taggcgagat cggcgccgaa gtaggtccag
     3121 tagccgtcgg acttcttgat cggacggtcc tgatcgtcgc cgaacttcgt cgagcggaac
     3181 agggggagtt cgaccggttc ccaatcttcc ggcgtcttgc ccttgggggc ttcgagcagg
     3241 ccgtcgtaga ccagatcgtg ggcgcgcagc caggcttcgg cggcatcgac cttgcccgag
     3301 gcctgcagct cggcctcgga ggcgaagacc tcgtgggtga tgccgaggag ggcgagatcg
     3361 gcgcggatca tctccatcat cgcggcgacg gcgcgggtgc ggaacagggc gagccattcg
     3421 gcttcggggg ccttggcata cctgtcgccg aattcggcgg cgaggtcctg tccgaccggg
     3481 accaggtaat cgcccgggta gaggccctcg gggatctc
//
LOCUS       DATNBV010000479         4197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2187683,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000479 DATNBV010000000
VERSION     DATNBV010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187683"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1347)
                     /locus_tag="VN627_11295"
     CDS             complement(127..1347)
                     /locus_tag="VN627_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03881.1"
                     /translation="MIILFRRIAVLLLLATGTLAAAQSVANLPDAAIERSGNVYHRAV
                     CSRAIGQGEARCMAHVRTDAYGRPLAGKPDGAAPNVTPSGYGPTALRSAYNVTATGSS
                     ATVVAIVDAYGYPNAESDLATYRSTMGLPPCTSASGCFIKLDQRGGTAYPRLNTGWAQ
                     EQALDLDMASAMCPNCRIMLVQSNSASYADLATAVNLAAARGALVISNSYGGGESGSA
                     SYNSAYTHPGVAITASTGDSGYGASFPATSPGVIAVGGTSLVRAANTRGWSESAWSGG
                     GSGCSSNYAKPVWQTDGLCARRMEADLSAVADPATGVAVYGPTGIGSKSGWLVFGGTS
                     VSAPLIGGIYGALGLGAANGASKIWATAATGTGFNNVTVGSNGACSGLYLCTAGVGYD
                     GPTGWGSPSGAAGF"
     gene            complement(1492..2721)
                     /locus_tag="VN627_11300"
     CDS             complement(1492..2721)
                     /locus_tag="VN627_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXD03882.1"
                     /translation="MSGADVVIVGGGHAGAQAAIALRQNGFTGTVTMIGRESEPPYER
                     PPLSKEYFAREKAFDRLYIRPPHFWGEKDVDLRLGVEVTKVDPAGKVLTLSDGTSFTY
                     GTLIWAAGGDPRRLTCKGADLAGVHAVRTRADCDQLMGEIDGGVKSIVVIGGGYIGLE
                     AAAVLTKLGCKVTLLEALPRVLARVAGPELSAFYEKEHRDHGVDLRTGVAVDCLEGEG
                     RVTGVKLADGSVIPAEAVIVGIGIVPAVGPLIAAGAAGANGIDIDEYCRTSLPDIYAV
                     GDCAAFACDFAGGTVMRVESVQNANDQATCVAKAICGEEKPYHAFPWFWSNQYDLRLQ
                     TAGLSVGYDATVTRGDPAARSFSVIYLKGGKVIALDCVNATKDYVQGRKLVEAGASPD
                     PAQLANAEVPLKELLPA"
     gene            complement(2861..3262)
                     /locus_tag="VN627_11305"
     CDS             complement(2861..3262)
                     /locus_tag="VN627_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HXD03883.1"
                     /translation="MAIATLLGASALLAGCGSSGGLLGRARPDEFAVQRQAPLVVPPD
                     FALTPPQPGAPRPNDTSAQQQTLDALFGGPAPRSEVEQATIGQAGAAAPGIRSAVGDP
                     QTHTVAKGALTRDIVAAPQGDGREAQAVIPG"
     gene            complement(3274..3822)
                     /gene="lspA"
                     /locus_tag="VN627_11310"
     CDS             complement(3274..3822)
                     /gene="lspA"
                     /locus_tag="VN627_11310"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HXD03884.1"
                     /translation="MTLGGRRLAGFGIAALVLAADQLVKWLVAGPLRLAEVQVIDLVP
                     IFDLRWAQNYGVSFSLLSASTNESRWLLVGLTGLIAAGVVVWLLREKHLPEVAALGMV
                     LGGALGNIHDRATLGYVVDYADLHFGDFRPFQIFNLADAAISIGVVIILARSFLSREK
                     RGDDDDAGSQPAPGAPEGESLN"
     gene            complement(3819..>4197)
                     /locus_tag="VN627_11315"
     CDS             complement(3819..>4197)
                     /locus_tag="VN627_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HXD03885.1"
                     /translation="SGSVHLLEWPEIDSAEAPDLFDRWTSIRGVRALVNEAIEPLRRE
                     KVVGSGLEADVTIGSLIAIAPGFSNEALAEVFITAKAEKGQGNFVTVSRTHNHKCGRC
                     WRHLPEVSDDGALCDRCEDVVGA"
BASE COUNT          681 a         1478 c         1387 g          651 t
ORIGIN      
        1 atcgcaccag aagccgaacc agcaccattc ttgccctcag gcgcacgcaa tgtcccccct
       61 caaggaaccg gccggccctc gctcccgatg cgatggccgg ccgggtggat cagaaatgag
      121 gccggatcaa aaccctgccg ccccgcttgg cgaaccccag ccggtcggcc cgtcatagcc
      181 caccccggcg gtgcacaggt agagccccga gcacgcgccg ttgctgccca cggtcacgtt
      241 gttgaagccc gtgccggtgg ccgccgtggc ccagatcttg gaggcgccgt tcgccgcgcc
      301 aaggccgagc gcgccataga tgccgccgat cagcggggcc gaaacgctcg ttcccccgaa
      361 gaccagccag cccgacttgg agccgatgcc ggtcgggcca tagacggcga cgccggtcgc
      421 cgggtcggcc acggccgaaa ggtcggcttc catgcgccgg gcgcaaagtc cgtcggtctg
      481 ccacacgggc ttggcatagt tgctggaaca gccgctcccg ccgcccgacc acgcgctttc
      541 cgaccagccg cgggtattgg ccgcccgcac cagcgaggtg ccgccaaccg cgatcacgcc
      601 cggcgaggtc gcggggaagc tggcgccata gccgctgtcg ccggtcgagg cggtgatcgc
      661 cacaccggga tgggtatagg cggaattgta agaagccgat ccgctttcgc cgccgccata
      721 ggaattggag atgaccagtg cgccgcgcgc cgcggcgagg ttaaccgcgg tggcaaggtc
      781 ggcgtaggac gccgagttcg actggaccag catgatccgg caattcggac acatggcgct
      841 ggccatgtcg aggtcgagcg cctgctcctg ggcccagccg gtattgaggc ggggataggc
      901 cgtgccgcca cgctggtcga gcttgatgaa gcagccgctg gccgaggtgc agggcggcag
      961 gcccatggtc gaccggtaag tggccagatc gctttccgcg ttcggatagc cataggcatc
     1021 gacaatggcg accaccgtcg ctgaactgcc cgtcgcagtc acgttgtagg ccgagcgcag
     1081 cgcggtcggg ccatagcccg aaggggttac gttgggcgcg gctccgtcag gctttccggc
     1141 cagcgggcgg ccataggcgt cggtgcggac atgggccatg cagcgcgctt cgccctggcc
     1201 gatcgcgcgc gagcacactg cgcggtggta gacattcccg ctgcgctcga ttgctgcgtc
     1261 gggaagattg gcgaccgatt gcgcggcggc cagggtgccg gttgccagca gcaacagcac
     1321 ggcgatacgt ctgaatagta tgatcacgta gcggccctcc ttggcccagc gcccggttct
     1381 cccaaaccag cactgaagcc tttcccctaa gggcacctta gcgattatcc ttacctgctg
     1441 tcagccatgt taggccgttt acggcctact gccgtaatca gcggcttcgt ttcaggccgg
     1501 gagcagttcc ttcaacggaa cctcggcatt ggccagctgc gccggatcgg gcgaggcgcc
     1561 ggcttccacc agcttgcggc cctggacata gtccttcgtc gcattcacgc agtcgagcgc
     1621 gatgaccttg ccgcccttga ggtagatcac cgaaaacgag cgcgccgccg gatcgccgcg
     1681 cgtgaccgtg gcgtcatagc cgaccgacag cccggcggtc tgcagcctca ggtcatactg
     1741 gttcgaccag aaccacggga aggcgtggta gggcttttcc tcgccgcaga tcgccttggc
     1801 gacgcaggtc gcctggtcat tggcgttctg gaccgattcc acccgcatca ccgtgccccc
     1861 ggcaaagtcg caggcaaacg ccgcgcagtc gcccaccgcg taaatgtcgg gcagcgaggt
     1921 gcggcaatat tcgtcgatgt cgatcccgtt ggcgccggcc gcgccggccg cgatcagcgg
     1981 gcccacggcg gggacgatgc cgatccccac gatgaccgcc tcggccggga tcacgctgcc
     2041 gtcggcgagc ttcacgccgg tgacgcggcc ctctccctcg aggcagtcca ccgccacgcc
     2101 ggtgcgcagg tccaccccgt ggtcgcggtg ttccttttcg tagaaggccg aaagctccgg
     2161 cccggccacg cgggccagca cgcggggcag cgcctccagc agggtcacct tgcagcccag
     2221 cttggtcagc accgccgccg cctcgagccc gatatagccg ccgccgatga cgacgatgga
     2281 cttcaccccg ccatcgatct cgcccatcag ctggtcgcag tcggcgcggg tgcgcacggc
     2341 gtggacgccg gccagatcgg cccccttgca ggtcagccgc cgcggatcgc cgcccgcggc
     2401 ccagatcagc gtgccatagg tgaagctggt gccgtcagag agcgtcagca ccttgcccgc
     2461 cggatcgacc ttggtgacct cgacgccgag acggaggtcc acatccttct caccccagaa
     2521 gtgcggcggg cggatgtaga gccggtcgaa ggccttctcg cgggcgaagt attccttgga
     2581 gagcggcggg cgctcgtagg gcggctcgct ctcgcggccg atcatcgtca cggtcccggt
     2641 gaagccgttc tgccgcagtg cgatcgcagc ctgtgcgccg gcatggccgc cccccacgat
     2701 aacgacgtcc gctccgctca tgatcgctcc tgtccccatc tgtcccgcgc ccgcttatcg
     2761 ggcacggctc cgcctagcgc gctggccgga atggcgccag cacacattga tcttccccaa
     2821 aaccggtaag ccttggtccc gctgcggcga ccggccgggc tcagcccggg atcaccgcct
     2881 gggcttcgcg gccgtcgccc tgcggcgcgg cgacgatgtc gcgggtgagg gcgcccttgg
     2941 cgaccgtgtg ggtctgcgga tcgcccacgg ccgagcggat gcccggcgcg gcggcaccgg
     3001 cctggccgat ggtcgcctgc tccacttcgc tgcgcggggc cgggccgccg aacagggcgt
     3061 cgagcgtctg ctgctgggcg ctggtgtcgt tcgggcgcgg cgcgccgggc tggggcgggg
     3121 tcagcgcgaa gtcgggcggg accaccagcg gggcctgccg ctgcacggcg aactcgtccg
     3181 ggcgggcgcg gccaaggagg ccgccgctcg agccgcagcc ggcaagcagg gccgaagcac
     3241 caagcagggt tgcgatagcc atggtcttgc gcatcagttc aaactctcgc cttcaggggc
     3301 gccaggcgcc ggttggctgc ccgcgtcatc gtcatcgccg cgcttttcgc gcgaaaggaa
     3361 cgagcgggca aggattatca ccacgccgat gctgatcgcc gcatcggcca ggttgaagat
     3421 ctggaacgga cggaaatcgc cgaagtggag gtcggcatag tcgacgacat agcccagcgt
     3481 cgcccggtcg tggatattgc cgagcgcgcc gcccagcacc atgcccagcg cggcgacttc
     3541 cggcaggtgc ttctcgcgca gcaaccagac caccaccccc gccgcgatca gcccggtgag
     3601 gccgaccagc agccaccggc tctcgttggt cgaggccgag agcagcgaga aggaaacccc
     3661 atagttctgc gcccagcgca ggtcgaagat cggcaccagg tcgatcactt gcacctcggc
     3721 cagccgcagc ggcccggcga ccagccactt gaccagctgg tccgccgcca gcaccagcgc
     3781 cgcaatgccg aacccggcca gcctgcgccc gccaagcgtc atgcgccgac cacgtcctcg
     3841 caccggtcgc acagcgcgcc gtcgtcgctc acctcgggca ggtggcgcca gcagcggccg
     3901 catttgtggt tgtgagtgcg agaaacggtc acgaaattgc cctgaccctt ttccgctttc
     3961 gccgtgatga acacctcagc gagcgcctca tttgagaacc cgggcgcgat ggcaattaga
     4021 cttccgatgg taacgtctgc ctcgaggccg gaacccacga ccttttcacg ccgaagcggt
     4081 tcaatcgctt cattaaccag tgcgcggacg cctctaattg aagtccatcg atcgaaaaga
     4141 tctggtgctt ccgccgaatc aatctccggc cattccagca ggtggacgct gccgctg
//
LOCUS       DATNBV010000480         2441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1557856,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000480 DATNBV010000000
VERSION     DATNBV010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557856"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..562)
                     /gene="msrB"
                     /locus_tag="VN627_11320"
     CDS             complement(65..562)
                     /gene="msrB"
                     /locus_tag="VN627_11320"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXD03886.1"
                     /translation="MTQPTDLSRRHALRLLAMGAAIPVLAACGSGEADARPYPVRFTD
                     AEWRKRLTAAQFHILREKGTEYPGTSPLLAEHRKGTFACAADGNPLFSSATKFESGTG
                     WPSFWQPLPGAVGYSADWDLGGKRTEVHCSRCGGHLGHVFDDGPKPTGKRYCMNGAAL
                     KFVVG"
     gene            complement(649..1884)
                     /locus_tag="VN627_11325"
     CDS             complement(649..1884)
                     /locus_tag="VN627_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXD03887.1"
                     /translation="MTGPGTNKSGGRLSTLAAALVIARRDFAAVLLSRSFIFFLIGPL
                     FPLVVGAMATGVGQSVERSAARPVLGVAMSPADTAALRGAASRLDAQLEDTVPVLLPL
                     EAQAGADPQALLQANQAKASAVLSGSLAQPVLTGPPGLIDDWRGPVAVLAAAAQSAAP
                     QPYPQVRLAPTTAKADPVDRVGRMRTAQAGQTLLFLLTMLLAGMVLSNLVEEKANKII
                     EVLAAAIPMDALFLGKLFAMLAVSFVGIAVWGTVAGTVLLAGGRTLAELTAPAVGWPM
                     FFALGVVYFSMAYLLLGSVFLTIGSMASTVREVQTLSMPVTMLQLLVFFFASFSVATP
                     GSPMEQAAALVPFSSPYAMLARAAEQPDLGQHLLAIAWQVLWVGLFIRFGSRLFRTRV
                     MKSGPAGTRRRRWFRRAPA"
     gene            complement(1881..>2441)
                     /locus_tag="VN627_11330"
     CDS             complement(1881..>2441)
                     /locus_tag="VN627_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXD03888.1"
                     /translation="QLSKGMAQQVQILGCLVHEPRLVVFDEPFSGLDAINQGKLEKTM
                     RGLADKGTTVIFSTHVIAHAERLCDGIAIIAGGKVPFAGPVDVARDRIPAQVRLETRA
                     ADGPWRAALPADARHDRKLDGTSFFYFPLPETGIEPLLRALIEGEAGILSLSIERAGL
                     HDAFVAIAGEAAARALEENPEEEQRR"
BASE COUNT          408 a          858 c          836 g          339 t
ORIGIN      
        1 atcggcgata ccgggttccc tcctcccacc tgcgggcggg gagtaaggca gcggttccct
       61 ctcgtcaccc caccacgaac ttaagcgccg ctccgttcat gcaatagcgc ttgccggtcg
      121 gcttgggccc gtcgtcgaac acatgcccca ggtgcccgcc acaccggctg cagtgcactt
      181 cggtgcgctt gccgcccaga tcccagtcgg ccgaataacc cacggcgccc ggcagcggtt
      241 gccagaagct gggccagccg gtgcccgatt cgaacttggt cgccgatgaa aacagcggat
      301 tgccatccgc cgcgcaggcg aaagtgccct tgcggtgctc ggccagcagc ggcgaggtac
      361 cgggatattc ggtgcctttc tcgcgcagga tgtgaaactg cgccgccgtc agccgcttgc
      421 gccactcggc gtcggtgaag cgcaccggat agggcctggc atcggcttcg cccgagccgc
      481 aggcggcgag cacgggaatg gcggcgccca tcgccagcag gcgcagggcg tggcggcggg
      541 acaggtcggt aggctgggtc atcgcggcga ttccttcgcc ccgatcatat ccgcgaacgc
      601 gcccgtgggg gagtgccctt cagggccacg agctcagacc cggaaagctc aggccggcgc
      661 ccgccggaac caccgccggc gccgcgttcc ggccgggccg ctcttcatca cccgggtgcg
      721 gaacagccgc gagccgaagc ggatgaacag gccgacccac agcacctgcc aggcgattgc
      781 cagcagatgc tggccgaggt ccggctgctc cgccgcgcgg gcgagcatgg catagggcga
      841 tgagaacggc accagcgccg ccgcctgctc cattggcgat cccggcgtcg ccacgctgaa
      901 gctggcgaag aagaacacca gcaactgcag catggtcacc ggcatggaga gcgtctgcac
      961 ctctcgcacg gtcgaggcca tcgatccgat ggtcaggaac accgaaccga gcagtaggta
     1021 ggccatcgag aagtagacca cgcccagtgc gaagaacatc ggccagccca cggccggcgc
     1081 ggtgagttcg gcaagggtcc ggccgccggc cagcaagacc gtgcccgcta ccgtgcccca
     1141 gactgcgatg ccgacgaagc tcactgccag catggcgaac agcttgccca ggaacagcgc
     1201 atccatcgga atcgcggcgg ccagcacctc gatgatcttg ttggcttttt cctcaaccag
     1261 attggaaagg accatgcccg ccagcagcat cgtcaggagg aacaggagcg tctgcccggc
     1321 ctgcgccgtc cgcatccgtc cgacgcgatc gaccggatca gccttcgccg tggtgggcgc
     1381 aaggcgcacc tgcggatagg gctgcggcgc ggcgctttgc gcggcggcgg ccagcacggc
     1441 gacagggccg cgccagtcgt cgatcaggcc cggcggcccg gtcagtaccg gctgggcaag
     1501 gctgcccgaa agcactgcgc tggccttggc ctggttcgcc tgaagcaggg cttgcggatc
     1561 ggcgccggcc tgtgcctcca ggggcaggag cacgggtacg gtatcctcca gctgcgcatc
     1621 cagccggctc gcggctccgc gcagggcggc cgtatcggcc gggctcatcg ccacgcccag
     1681 cacgggccgc gccgcgctgc gctcgacgct ctggcccacg cccgtcgcca tggcgccgac
     1741 caccagcgga aacagcggcc cgatcaggaa gaagatgaag ctgcgcgaaa gcagcacggc
     1801 ggcgaagtcg cgccgcgcga tcaccagcgc tgccgcaagg gtggacaggc gtcccccgga
     1861 tttgttggtg ccggggccgg tcatcggcgc tgctcctctt cggggttctc ttccagcgcc
     1921 cgcgccgccg cctcgccggc gatggcaaca aaggcatcgt gcaggcccgc ccgctcgatc
     1981 gagagcgaga ggatccccgc ctcgccctcg atcagggcgc gcagcagcgg ctcgattccg
     2041 gtttcgggca gggggaaata gaagaacgag gtgccgtcga gcttgcggtc atgccgcgca
     2101 tcggcgggca gggcggcgcg ccacggccca tccgccgcgc gcgtctccag ccggacctga
     2161 gcggggatgc gatcgcgcgc cacgtccacc ggcccggcaa agggcacctt gccgccggca
     2221 atgatcgcga tcccgtcgca cagccgttcg gcatgggcga tgacgtgggt ggagaagatc
     2281 acggtcgtgc ccttgtcagc caggccgcgc atcgtcttct ccagcttgcc ctggttgatc
     2341 gcatcgagcc ccgagaaggg ctcgtcgaat acgaccaggc gcggctcgtg aacgaggcag
     2401 ccgaggatct ggacctgctg ggccatgccc ttggacagct g
//
LOCUS       DATNBV010000481         3998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_908849,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000481 DATNBV010000000
VERSION     DATNBV010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908849"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /gene="nusG"
                     /locus_tag="VN627_11335"
     CDS             <1..409
                     /gene="nusG"
                     /locus_tag="VN627_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HXD03889.1"
                     /translation="VTEVKRGKKIQVERKFMPGYVLAKLALNDDVYHLVKNTPKVTGF
                     LGPNGKPQAISDREAARYFGARDQAAAEPRREVSVDYEIGDSVKVNAGPFASFNGTVE
                     ELDFDKQRVKVSVSIFGRATPVELGFEEVELVK"
     gene            563..1534
                     /locus_tag="VN627_11340"
     CDS             563..1534
                     /locus_tag="VN627_11340"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXD03890.1"
                     /translation="MARGRLSGLDSLRGIAAVVVLTYHVLAGYGTNGFLAVDFFFMLS
                     GYVMARTYEHRLGEPGAGGRFVMARIERLWPPMVLASLVGLPWLWWHVPDRALHVAIA
                     NLLLLPTPTPDDIYLLNPPAWSILFELIANGVHAAILWRLSNRQLVGLLAVLVPLLTW
                     FAFAYTMHMVTPDHLFTRGMVRLLCPYVIGVLLYRCWRDEPPLRVPPAVSWALLPAFF
                     IGGNWLWGSIWLPDLLFVLVLCPLAIAGGLTMPASRLGSWLGAISFPLYAIHGPLLML
                     MREFGWSLPVQFVFALGGGIAFTGLLAGIARLMKRVDNSAPAVLREA"
     gene            1537..2577
                     /locus_tag="VN627_11345"
     CDS             1537..2577
                     /locus_tag="VN627_11345"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXD03891.1"
                     /translation="MACISTAAPSRLARLDGLRGLAALGVTLHHLFLHYAPWAGPGEA
                     IAAWLRGWGWSLVDLFFVLSGYVFAHVYLPGGRLAGPQGLRDFALARFARLYPLHLAT
                     LLAVAAVMFGRPNNGPVELGANLLMLQAVLHPFAQSFNQPSWSLSVEAFCYVVFALAA
                     QAGRQRLLQVTAGAVAFSALYLAALGRAEIADAFPRGFLGFFAGQALWHLRARLAAVP
                     TAALAAALVLGCLIAFAPAMHGSSPALALSLLAWPAALVLALRWPVMESRAMVWLGDR
                     SYAIYLINLPLIFAAGAVLGHEGGIPVHLALLAAILALSDLAWRFLEMPARRAIVAAG
                     RAKGLGIRPQLG"
     gene            2713..3144
                     /gene="rplK"
                     /locus_tag="VN627_11350"
     CDS             2713..3144
                     /gene="rplK"
                     /locus_tag="VN627_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HXD03892.1"
                     /translation="MAKKIEGYIKLQVAAGAANPSPPIGPALGQRGVNIMEFCKAFNA
                     ATQELEKGMPIPTVITVYSDRSFSFVTKTPPASFLIKKAANLKSGSKEPGKVSAGTIK
                     RSQLAEIAQTKMADLNANDIDQATKIIEGSARSMGLQVVEG"
     gene            3149..3850
                     /gene="rplA"
                     /locus_tag="VN627_11355"
     CDS             3149..3850
                     /gene="rplA"
                     /locus_tag="VN627_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HXD03893.1"
                     /translation="MAKQTKKQKALEAKLGDNQKLYGIDEAIALLKDLKSAKFDETLE
                     VSLNLGVDPRHADQMVRGMVTLPSGTGKDVKVAVFARGDKAEAALAAGADKVGAEDLM
                     EDMLAGNLDYGRVIATPDMMGVVGRLGKTLGPKGLMPNPKLGTVTPNVAEAVKAAKGG
                     QIEFRVEKAGIIHGGLGKLSFSNEALRANFDAFLDAIVKAKPAGAKGKYLRKAALSSS
                     MGPGIKLDVAEVHGA"
BASE COUNT          574 a         1337 c         1348 g          739 t
ORIGIN      
        1 cgtgaccgaa gtgaagcgcg gcaagaagat ccaggtcgaa cgcaaattca tgcccggcta
       61 cgtgctggcc aagctggcac tgaacgacga cgtgtaccac ctcgtcaaga acacccccaa
      121 ggtgaccggc ttcctcggcc ccaacggcaa gccccaggcg atcagcgacc gcgaggccgc
      181 ccgctacttc ggcgcgcgcg accaggccgc cgccgagccg cgccgcgaag tttcggtcga
      241 ttacgagatt ggcgattctg tcaaggtcaa cgccgggccc ttcgccagct tcaacggcac
      301 ggtggaagag ctcgacttcg acaagcagcg ggtcaaggtc tcggtctcga tcttcggccg
      361 cgccaccccg gtggaactgg gcttcgagga agtcgaactg gtcaagtagg agtcgcttgc
      421 gccacccagt gtggccgggt ggcgcaaaat gcgccctagc tgacatcgtg gtccccgtgc
      481 ctgtccggct tggggtgcgt gaaggcgctt ccgcgcggtc cgcgtcaact aattgttagc
      541 catgccgggc ctaggacagg gcatggcaag ggggcgcctt tcgggattgg acagcttgcg
      601 cgggatcgcc gcggtggtcg tgctgaccta tcacgttctc gcgggctacg gcaccaatgg
      661 gttcctcgcg gtcgacttct tcttcatgct gtcgggctat gtcatggccc ggacctacga
      721 gcatcggctg ggcgagccgg gcgccggcgg gcgcttcgtc atggcgcgga tcgagcggct
      781 gtggccgccg atggtcctgg cgagcctcgt cgggctgcca tggctgtggt ggcacgtgcc
      841 cgaccgcgcg ctgcacgtcg cgattgccaa tctgctgctg ctgcccacgc ccacgcctga
      901 cgatatctat ctgctcaatc cgcccgcctg gtcgatcctg ttcgagttga tcgccaacgg
      961 cgtacatgcg gcgatcctgt ggcggctgtc caaccggcaa ctggtcggtc tcctggccgt
     1021 gctggtccct ttgctgacgt ggttcgcctt cgcctacacc atgcacatgg tgacgcccga
     1081 tcacctgttc acgcgcggca tggtgcggct gctgtgtcct tatgtcatcg gcgtgctgct
     1141 ataccgctgc tggcgcgacg agccgccgct gcgggtgcct ccggcggtga gctgggccct
     1201 gctgccggcc ttcttcatcg gcggcaattg gctgtggggg tccatctggc tgcctgatct
     1261 gctgttcgtg ctggtgctct gtcccctcgc gatcgccggt ggcctgacca tgccggcatc
     1321 gcgcctgggt tcgtggctgg gcgccatctc gttcccgctc tatgccattc acggcccgct
     1381 gctgatgctg atgcgcgagt tcggctggtc gcttccggtg cagttcgtct tcgcgctggg
     1441 cggcgggatt gccttcaccg gcctgttggc cggaattgcg cggctgatga agcgggtgga
     1501 caattctgca ccggccgtgc tgcgcgaggc ctagccgtgg cctgtatttc aaccgcggcg
     1561 ccttcgcgtc tcgcccggct tgacgggctg cgcggcctgg cggcgcttgg cgtgacgctg
     1621 caccacctgt tcctgcacta tgctccatgg gccgggccgg gggaggcgat tgccgcgtgg
     1681 ctgcgcggct ggggctggtc cctcgtcgac ctgttctttg tcctgtcggg ttatgtcttc
     1741 gctcatgtct acctgcccgg cgggcggctg gccggccccc agggactgcg ggatttcgcg
     1801 ctggcccggt tcgcccggct ctatccgctg cacctggcga cgctgctggc ggttgcggcg
     1861 gtgatgttcg gccggccgaa caacggcccg gtggagctgg gggccaacct gctcatgctg
     1921 caggccgtgc ttcatccttt cgcccagagc ttcaaccagc cttcgtggtc gctctcggtc
     1981 gaggcgttct gttatgtggt tttcgcgctg gcggcgcagg ccggccggca gcgcctgctt
     2041 caggtcacgg cgggggccgt cgccttttcc gcgctctatc tcgccgcgct cggccgggcg
     2101 gaaattgccg atgcctttcc ccgcgggttc ctcggctttt tcgcgggaca ggcattgtgg
     2161 cacctgcgcg cgcgcctggc ggcggtgccc acggcagcgc tggcggcagc gctggtcctg
     2221 ggctgcctga tcgccttcgc cccggccatg cacgggtcca gcccggcttt ggccctgtcc
     2281 ctgcttgcct ggccggcggc cctggtgctg gcgctgcgct ggccggtgat ggaatcgcgg
     2341 gcgatggtct ggctgggcga ccggtcctat gcgatctacc tgatcaacct gccgctgatc
     2401 ttcgccgccg gcgccgtgct cggccacgag ggcgggatcc cggtgcacct ggccctgctg
     2461 gcggcgatcc tcgcgctcag cgatctggcc tggcgcttcc tcgaaatgcc ggcgcggcgg
     2521 gccattgtgg cagcaggccg cgcgaagggc cttggcatcc gcccgcaatt gggctaggcg
     2581 cgcgcccttc gtccgggtgc tcattgcgta cccatcgggc gattcccggc ggaagggcgc
     2641 agccgcgcct gtttgaccgc tcactctgag gcgcgggttc gcctgcgcca aaaggaagaa
     2701 aggaggccct ccgtggccaa gaagatcgaa ggttacatca agctgcaggt tgcagccggt
     2761 gccgccaacc cctcgccgcc gatcggtccg gcgctgggtc agcgcggtgt caacatcatg
     2821 gaattctgca aggcgttcaa cgccgccacg caggagctgg aaaagggcat gccgatcccg
     2881 acggtgatca ccgtctattc ggaccgctcg ttcagcttcg tcaccaagac gccgcccgct
     2941 tcgttcctga tcaagaaggc cgccaacctc aagtcgggct cgaaggagcc gggcaaggtc
     3001 agcgccggaa cgatcaagcg ctcgcagctc gccgagatcg cccagaccaa gatggccgac
     3061 cttaacgcga acgatatcga ccaggccacc aagatcatcg agggctcggc ccgttcgatg
     3121 ggcctgcagg ttgtggaggg ctgatcacat ggcaaagcag accaagaagc agaaggccct
     3181 cgaagccaag ctgggcgaca accagaagct ctacggcatc gatgaagcca tcgcgctcct
     3241 gaaggacctc aagtccgcca agtttgacga gaccctcgag gtttcgctga acctcggcgt
     3301 cgatccgcgc cacgccgacc agatggtccg cggcatggtc accctgcctt cgggcaccgg
     3361 caaggacgtc aaggttgccg tcttcgcgcg cggcgacaag gctgaggccg ctctcgccgc
     3421 cggggccgac aaagtcggtg ccgaagacct catggaagac atgctggccg gcaacctcga
     3481 ctatggccgc gtgatcgcca cgccggacat gatgggcgtc gtcggccgtc tcggcaagac
     3541 gctgggcccc aagggcctga tgccgaaccc gaagctcggc accgtcaccc cgaacgtcgc
     3601 cgaagcggtc aaggctgcca agggcggcca gatcgagttc cgcgtcgaga aggcgggcat
     3661 catccacggc gggctaggca agctgtcgtt ctcgaacgag gcgctgcgcg ccaacttcga
     3721 cgcgttcctc gatgccatcg tcaaggcgaa gccggccggc gccaagggca agtacctgcg
     3781 caaggccgcg ctgtcctcgt cgatgggccc gggcatcaag ctcgacgtcg cggaagtgca
     3841 cggcgcgtaa gttccgtcgt cccgggctcg gcccggggcc aacgaccgta agggccggga
     3901 ggggaaacct tcccggcctt ttcgtttgcg ccacaaccgc gcggtcagcg gtgaccgggg
     3961 gcgaggttcg cggcggtgta gcattcggtc accacgct
//
LOCUS       DATNBV010000482         3229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_909456,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000482 DATNBV010000000
VERSION     DATNBV010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909456"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VN627_11360"
     CDS             complement(<1..614)
                     /locus_tag="VN627_11360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HXD03894.1"
                     /translation="MGNAVALVVLAASIAELIANAFHPIPRDFLSFWGAAQLALAGHP
                     AAAYDSGALHAVQKAVADFGTGHAEMPFPYPPAYLALVAPFGLLPFPLAMAIWIACTF
                     AFYLWAARRLIPRSGWLAAAFPAVYANATDGQNAFLTVGLFMSGLALASRRSFAAGLV
                     LGCLIVKPQLALLLPVAFIAARSWRVIAGAAISSTGILLAGALLL"
     gene            complement(749..1402)
                     /gene="fsa"
                     /locus_tag="VN627_11365"
     CDS             complement(749..1402)
                     /gene="fsa"
                     /locus_tag="VN627_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HXD03895.1"
                     /translation="MKFFADTAEIADIKELAATGLLDGVTTNPSLIMKSGRDFMEVTK
                     EICGIVDGPVSAEVVALDHEGMMREAEVLRKIADNVCIKVPLTIDGLKTCKALTGEGT
                     MVNVTLCFSAVQALLAAKAGATFISPFVGRHDDNGFDGMQLIADIRLIYDNYDFPTEI
                     LVASVRHPVHVLEAAKIGADVMTAPPKIIWQLFKHPLTDAGIASFLKDWEATGQKIG"
     gene            1553..1996
                     /locus_tag="VN627_11370"
     CDS             1553..1996
                     /locus_tag="VN627_11370"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXD03896.1"
                     /translation="MSPANPHFRERIEQRLTMPLIADFFRMYAVAIEPGAVTIALDHR
                     LELGHEPGWFQGSVTTAIAEFAAGFSGQSLLAPDWAAVTLDQHINFVGAARGERLVAR
                     GRVIVAGRTISTCAAEVFVVRDGAEHLCATMVQTNRNAPLPPARP"
     gene            2062..>3229
                     /gene="priA"
                     /locus_tag="VN627_11375"
     CDS             2062..>3229
                     /gene="priA"
                     /locus_tag="VN627_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXD03897.1"
                     /translation="MRRVRLLVFNAALGPLDYRVPEGAEVEYGSLVIAPLGPRQVLGV
                     VWEPERLPATDVPDARLRPLLEVLPVPPLSAPLRRLIEWTSDYYCAPLAAVARMALGS
                     LAALRGGGTTTEYRLTGTVPARLTPQRAAAIDALHGEQASLRELAERAGVSDGVLRGL
                     VGVGALEPVTVDTDRPFPPADPGHAAPDLSDDQQAAAQTFAAAVRAGGFQPFLLDGVT
                     GSGKTECYFEGVAAAIEAGKQVLVLLPEIALTQNFLRRFEARFGVAPVLWHSSLKASE
                     RRRAWRAIVRGDAQVIVGARSALFLPYARLGLIVVDEAHEVSFKQDDGVRYNARDVAV
                     IRAKFEGIPVILASATPALESMQLAEAGVYRRIELAARYGGAELPAIRIVDLREA"
BASE COUNT          526 a         1060 c         1119 g          524 t
ORIGIN      
        1 agcagcaatg cgcccgcaag caatatgccc gtcgatgaga tcgcagcgcc ggcaatcacc
       61 cgccagctcc gggcggcgat gaaggccacc ggcagcagca aggcaagctg cggtttaacg
      121 atcaggcagc cgagaacgag cccggccgcg aacgatcgtc gagacgccaa tgcaaggccg
      181 ctcatgaaga gccctacagt caggaatgcg ttctgaccgt cggtcgcgtt cgcatagact
      241 gcgggaaagg ccgccgcgag ccacccagat cgcgggatca gcctgcgcgc ggcccacagg
      301 tagaaagcga atgtgcaggc gatccagatc gccatcgcaa gcgggaaagg cagcaacccg
      361 aacggagcca ccaacgcgag ataggccggc ggatagggaa acggcatctc cgcatgaccc
      421 gtgccgaaat cggcgaccgc cttctggaca gcatgcagcg cgccgctgtc gtaggcggct
      481 gcagggtgac ccgcgagcgc cagctgcgcg gccccccaga agctcaggaa atcgcgcgga
      541 atggggtgga aggcatttgc gatgagctcg gcgatcgatg ccgcaagaac gaccagcgca
      601 accgcattcc ccacgaaccc gtaaggagcg taagggtcgc cctccccggt ccgcggcaat
      661 gccgtgcctt ggtcactcgt aaatgccatc cgcccctacc ccgaacccgt tgtcggcgta
      721 ggattagcag aggatttcta aggaaggctt agccgatctt ctggccagta gcttcccagt
      781 ccttgaggaa gcttgcgatc cccgcgtcgg tcagcgggtg cttgaacagc tgccagatga
      841 tcttcggggg cgcggtcatc acgtccgcac cgattttcgc cgcttcgagg acgtgtaccg
      901 gatgccgcac ggacgcgacc aggatctccg tcgggaaatc gtaattgtcg tagataaggc
      961 ggatgtcggc gatcagctgc atgccgtcga agccgttgtc gtcatgccgg ccgacgaagg
     1021 gcgaaatgaa cgttgccccg gccttggcgg caaggagcgc ctggaccgcc gagaagcaca
     1081 gcgtgacgtt gaccatggtg ccctcgcccg tcaacgcctt gcaggtcttc aggccatcga
     1141 tggtgagtgg caccttgatg cagacgttgt ccgcgatctt gcgcagcacc tcggcctcgc
     1201 gcatcattcc ttcgtgatcg agcgcgacca cttcggcgct gaccgggccg tcgacgatcc
     1261 cgcagatttc cttggtcacc tccatgaaat cgcggcccga cttcatgatc agcgagggat
     1321 tggtggtgac gccatcgagc agtccggtgg cggccagctc cttgatgtcg gcgatttcgg
     1381 cggtgtcggc gaagaacttc acgggggcgc tccgggcaaa tgattccatc cgcctatagg
     1441 cagtgcgccg gggccccgcc acccccactt gcgaccatgt cccgcctgcc ccgcattgcc
     1501 cgccgctgcc gcaatcgcta ccgccgctgc cacaccatgg gggaggcctg caatgagccc
     1561 ggcaaatccg cactttcgcg agcggatcga acagcgactg acaatgccgc tgatcgcgga
     1621 tttcttccgc atgtatgcgg tggcgatcga gccaggggcg gtgacgattg ctctcgatca
     1681 ccggcttgag ctgggccatg agcccggctg gttccagggc agcgtgacga ccgccatcgc
     1741 ggagttcgcc gccggctttt cgggccagag cctccttgcc ccggattggg cggcagtcac
     1801 gctggaccag cacatcaatt tcgtgggcgc ggcgcggggc gagcggctgg tggcgcgcgg
     1861 gcgcgtgatc gtggcggggc ggacgatctc gacctgcgcg gcggaggttt ttgtggtgcg
     1921 tgacggggcc gagcatctgt gtgcgaccat ggtccagacc aatcgcaacg cgccgctgcc
     1981 gccggcccga ccctgagctt ccgaccggcg cgcgcccgat cgggggtggc ggagcgggac
     2041 gccccccaat atagccggcc catgcgccgc gttcgcctgc ttgtgttcaa tgccgccctg
     2101 ggaccgctgg attaccgtgt gccggaaggg gccgaggtgg aatacggcag tctggtcatc
     2161 gcgccgctgg ggccgcggca ggtgctgggg gtggtgtggg agcccgagcg gctgccggcg
     2221 acggacgtgc ccgatgcgcg gctgcggccc ttgctggagg tgctcccggt accgcccctg
     2281 tccgcgccct tgcggcggct gatcgagtgg acctcggact actactgcgc cccgcttgcc
     2341 gcggtggcgc gaatggcgct gggttcgctc gcggcgctgc gcgggggggg gacgaccacc
     2401 gaataccggc tgaccggcac ggtgcccgcg cgcctgacgc cgcagcgcgc cgcggccatc
     2461 gatgcgttgc acggagagca ggcgagcttg cgcgaactgg ccgagcgggc gggcgtttcg
     2521 gatggggtgc tgcgcgggct ggtcggcgtg ggcgcgctgg agccggttac ggtggatacc
     2581 gaccggccct ttccgcccgc cgatccgggc catgccgccc cggatctatc cgacgaccag
     2641 caggccgccg cgcagacttt cgccgcggcc gtgcgcgcgg gcggcttcca gccgttcctg
     2701 ctcgacggcg tgaccggatc gggcaagacg gaatgctatt tcgagggcgt cgccgccgcc
     2761 atcgaagccg gcaagcaggt gctggtcctg ctgcccgaaa tcgcgctgac ccaaaacttc
     2821 ctgcgtcggt tcgaggcccg ctttggcgtg gccccagtgc tgtggcactc cagcctcaag
     2881 gcgagcgagc gccgccgggc ctggcgagcg atcgtgcgcg gcgatgcgca ggtcatcgtg
     2941 ggcgcccgtt cggcgctgtt cctgccttat gcgcggttgg ggctgatcgt cgtcgatgaa
     3001 gcgcatgaag tctcgttcaa gcaggacgac ggggtgcgct acaacgcgcg cgatgtggcg
     3061 gtcatccggg ccaaattcga aggcatcccg gtaatcctcg ccagcgccac gcccgccctc
     3121 gaatcgatgc agctggccga ggcgggggtt taccggcgaa tcgagctggc cgcgcgctat
     3181 ggcggggccg agctgcctgc gatccggatc gtcgacctgc gcgaagccc
//
LOCUS       DATNBV010000483         2366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3771079,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000483 DATNBV010000000
VERSION     DATNBV010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3771079"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="VN627_11380"
     CDS             complement(<1..1407)
                     /locus_tag="VN627_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HXD03898.1"
                     /translation="MTGAQWLSILTLAGMMGLFLWDRFRYDVSAVMALLAAVALGVVK
                     PKDAFTGFSDDIVIIVGSALVISAAVQRSGKIEVMLSHLARRVRSAKSELVLLTGSVG
                     FASALVKNVGALSMLMPAAVQMARKRGASPSAFLMPMSFASLLGGLMTLVGTSPNIIV
                     SRVRMDMTGKPFGMFDYLPTGLGLLVVGLIFLRFAGPLIPAGRMAAPQVAEAVDVGAY
                     TTQALVPAGSPTVNETVRGFAKRHGGEMEIIGLIRDGARGAVHPFARLREGDVLILEG
                     EPDVLQQAIARDRLVLEGKDRRVPQGSERDEIEVVEAVVTRGSALVGKAAGRYFLHER
                     YGINLIAVSRAGERLDRKLAEIVLRAGDVVVLQGPSGLLPQRLKDLGALPLAERPIRL
                     GQRTRGWLPLAILGLAMAATATGTVPVAIAFFAAAGAVVIAGTLPVRAAYEAIDWSIL
                     IMLGALIPVSDSLRTSGAS"
     gene            complement(1404..1847)
                     /locus_tag="VN627_11385"
     CDS             complement(1404..1847)
                     /locus_tag="VN627_11385"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HXD03899.1"
                     /translation="MDGPPEKTPAASAGTQQLARSAGKLAKSAENQEDSADRRTELAA
                     DRTVLAAERTYAAWVRTGLSALASGVGAGALLDKILPALVGKLTGTLLIAFSAFCFGA
                     AVWRQMVRVAPPRPDTRRLPQALLIAVNGFLALVSLGALVGIWLA"
     gene            complement(1884..>2366)
                     /locus_tag="VN627_11390"
     CDS             complement(1884..>2366)
                     /locus_tag="VN627_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03900.1"
                     /translation="QVRRVANSAHVWNNFPAAVIQARIPVRYVPTVRGTRYAGQSRMN
                     FVSLVAHGLGAISVFSEAVFIRILVASALLLLLSLSLAGLSLFMKLVIQVAFPNWATT
                     VLGFALVISVQALMMPILMAFLLLNNRSLVQPLPRKIVLDLIDERRTLWCAPDCSHKA
                     "
BASE COUNT          410 a          862 c          780 g          314 t
ORIGIN      
        1 cgaggcgccg ctggtgcgca agctgtcgct caccggaatc agcgcgccca gcatgatcag
       61 gatcgaccag tcgatcgcct cgtaggccgc gcgcaccggc agggtgcccg cgatcaccac
      121 cgcgcccgcc gccgcgaaaa aggcgatcgc cactggcacg gtgccggtcg ccgtcgccgc
      181 catggcgagg cccaggatcg ccagcggcag ccatccgcgc gtgcgctggc ccagccggat
      241 cgggcgttcg gccaggggca gggcgccaag gtccttgagc cgctgcggca ggagcccgct
      301 cggcccctgc agcaccacca cgtcgcccgc gcgcagcacg atttcagcga gcttccggtc
      361 gagccgctcg cccgcgcggg aaaccgcgat caggttgatg ccatagcgct cgtgcaggaa
      421 atagcgcccg gccgccttgc cgaccagcgc cgaaccgcgc gtgaccaccg cctccacgac
      481 ctcgatctcg tcgcgttcgc tgccttgggg cacgcgccgg tccttgcctt ccagcaccag
      541 ccggtcgcgc gcgattgcct gctggagcac gtcgggttcg ccctccagga tcagcacgtc
      601 gccctcccgc agccgggcga aggggtgcac cgccccgcgc gcgccgtcgc ggatcagccc
      661 gatgatctcc atctccccgc cgtgccgctt ggcaaagccg cgcaccgtct cgttcacggt
      721 cggcgatccg gcggggacca gcgcctgggt ggtataggcc cccacgtcca ccgcttccgc
      781 cacctgcggc gcggccatcc ggcccgccgg gatcagcggc ccggcaaagc gcaggaagat
      841 gagccccacc accagcaggc caagaccggt cggcaggtag tcgaacatgc cgaagggctt
      901 gccggtcatg tccatccgca cccggctgac gatgatgttg ggcgaagtgc cgaccagcgt
      961 catcagcccg cccagcagcg aggcgaagct catcggcatg aggaaggccg aagggctcgc
     1021 cccccgcttg cgggccatct gcacggccgc gggcatcagc atcgagagcg cgccgacgtt
     1081 cttgaccagc gccgaggcga acccgacact gccggtcagc agcaccagct cgctcttcgc
     1141 gctgcgcacc cgccgggcga ggtgcgagag catgacttcg atcttgcccg aacgctgcac
     1201 cgccgcggaa atgacgaggg ccgagcccac gatgatcacg atgtcgtcgg aaaagccggt
     1261 gaaggcgtcc ttgggcttga ccacccccag cgccacggcg gcgagcaggg ccatgaccgc
     1321 gctcacgtcg tagcgaaacc ggtcccacag gaagaggccc atcatccccg ccagtgtcag
     1381 gatcgagagc cattgcgccc cggtcatgcc agccagatcc ccaccagcgc ccccaggctc
     1441 accagggcaa ggaagccgtt caccgcgatc agcagggcct ggggcagccg ccgcgtgtcg
     1501 ggccggggcg gggcaacgcg gaccatctgc cgccacaccg ccgcgccaaa gcagaaggcg
     1561 ctgaaggcga tcagcagcgt gcccgtcagc ttacccacca gcgcgggcag gatcttgtcg
     1621 agcagcgccc ccgcgccgac gcccgaagcc agcgcggaaa gccccgtccg cacccaggcg
     1681 gcataggtcc gctcggccgc cagaaccgtg cggtcagccg ccagctcggt ccgccggtcg
     1741 gcgctgtctt cctggttctc ggcacttttg gcgagcttcc ccgcgctgcg cgcaagctgc
     1801 tgtgttccgg ctgacgctgc cggggttttc tcgggaggtc cgtccaccat cgcacgccca
     1861 acgcgcgaag gcgcgatgcg ggttcaggcc ttgtgcgaac aatccggcgc gcaccacaag
     1921 gtccgacgct cgtcgatcag gtcgagcacg atcttgcgcg gcagcggctg gacgaggctg
     1981 cggttgttga gcaggaggaa ggccatgagg atcggcatca tcagggcctg aacgctgatc
     2041 accagggcaa agcccagcac ggtggtcgcc cagttgggga aagccacctg gatgaccagc
     2101 ttcatgaaca gcgacagtcc cgccaggctc agcgataaca gcagcagcaa tgcgctggcc
     2161 accaggatgc ggatgaatac ggcctcggag aacaccgaaa tggccccgag cccatgggcc
     2221 accaggctga cgaagttcat gcgcgattgc ccggcgtagc gcgtgcccct caccgtgggc
     2281 acgtatcgca ccggaatgcg cgcttggata acggccgcgg gaaaattgtt ccagacgtgg
     2341 gcgctgttgg caacgcggcg aacttg
//
LOCUS       DATNBV010000484         4005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2478962,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000484 DATNBV010000000
VERSION     DATNBV010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2478962"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..501)
                     /locus_tag="VN627_11395"
     CDS             complement(109..501)
                     /locus_tag="VN627_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HXD03901.1"
                     /translation="MTLFSKGTKRTIAAMKGAGVSRLAVITGVGAGESRGHGGLLYEW
                     IGLPFVIGAIYADKDRQEALVKASGLDWTIVRPTLLTNGPRTGQYRVLTSFEGLHGGR
                     ISRADCADCLLRAMVEGTWSQTAIVITE"
     gene            645..1769
                     /locus_tag="VN627_11400"
     CDS             645..1769
                     /locus_tag="VN627_11400"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXD03902.1"
                     /translation="MDQNSTIIAVIEMSRSNWLVAGVIPGVDRQPLKKLEPDEEGLLH
                     LLRRWQDEAARAGRTISRIAVAFEAGRDGFWLARWLRARGIEAYVIHPASIPVSREHR
                     RAKTDRLDTELLKRAFLGWLRGEPNHCGMAAVPTLEQEDAKRPNREREVLVGERTRIV
                     NRMKGTLARLGIRGFKPALPRAPERLETLRTPEGATLPPNTRAELQRDMARLRFVTDQ
                     IRELEAARAERLEHAPETGPNAMIRLLARVVGVGIETADMLVQEVLSRNLRDRRAVAR
                     YAGLTGAPDESGAKRREKGLAKAGNARVRRGMIQLAWRFLLFQKDSALSQWYRARTTD
                     ARGATRKTLIVALARKLLIALWRFGATGEVPQGVVLRPAA"
     gene            2213..2605
                     /locus_tag="VN627_11405"
                     /pseudo
     CDS             2213..2605
                     /locus_tag="VN627_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2998..>4005)
                     /locus_tag="VN627_11410"
     CDS             complement(2998..>4005)
                     /locus_tag="VN627_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HXD03903.1"
                     /translation="TFGAVTVCVPRARLATPEGGTKEWRNASLPAYQRLTRRAEALIA
                     GAYLSGTNTRRVRRALAALFGGAVGKDVVSRVWRKVKTDWDAWTRRDLSAEPIIRLIL
                     DGTVVRVRLDRKATAISLLVVLGVRQDGQKVLLAVKNMGGESEAAWRAILEDLVARGL
                     RTPEFVIVDGAPGLEKALAALWPDVPAQRCTVHKHRNLLAHAPDRLHEEISADYTDMI
                     YAASAAEVAERRKAFLRKWRLKCQAVADSLEEAGDKLFTFTRLPPSQWKSARTTNAVE
                     RLHEEFKRRIKTQTVLPSAETAAMLFWALLASGQITMRKVDGWPTLAEKPADQPIDLA
                     A"
BASE COUNT          680 a         1304 c         1351 g          670 t
ORIGIN      
        1 gagcagcctc tttgggtcgg tcgctggctg ccgggaggtc tttcagaagg cctgagacaa
       61 gccggtaaag gacagaacag acgccgggcg aaccgcccaa gcctgcttct attctgtgat
      121 cacaattgcc gtctgcgacc atgtcccctc gaccatggcg cgaagcaggc aatctgcaca
      181 gtcagcccgg ctgatccggc cgccatgaag tccctcgaag ctcgtcagga cccggtactg
      241 acccgtacgc gggccattgg tgagcagtgt tggacgaaca atggtccagt cgagaccgga
      301 cgccttcaca agcgcctctt gacgatcctt atcggcgtaa atggccccga tgacgaaggg
      361 taatccaatc cactcgtata gaagaccgcc gtgccctcgg ctctcacccg ccccgacccc
      421 ggtgatcacg gcaagccggc tcaccccagc ccctttcata gccgcaatgg tccgtttggt
      481 tcctttagag aagagggtca ctgtgtgcag gaaggcggga ccgagggttc taacattaca
      541 gaggctatgg ggcgggcggc gaggcggagc accatcgtcg ccgggaacca ccgatggagc
      601 gccccatgcc acagcggaat gacctgagta gatctctcct tgcgttggac cagaacagca
      661 cgattatcgc cgtcatcgag atgagccggt cgaattggct cgttgccggg gtcataccag
      721 gcgttgaccg ccaaccactg aagaagcttg agccagacga ggagggcctt ctgcacctgc
      781 tccgacgctg gcaggacgaa gcggccagag caggacgaac catcagccgg attgccgttg
      841 cattcgaggc cggtcgcgac ggcttctggc ttgcccgctg gctgcgggcc cgcgggatcg
      901 aggcgtacgt gatccatccg gccagcattc cggtttcgcg ggagcaccgg cgcgccaaga
      961 cggatcggct cgacacggag ctgctcaagc gcgctttcct gggctggctg cgcggcgagc
     1021 caaaccactg cggcatggcc gcggtgccca cgctcgagca ggaggacgcc aagcgtccga
     1081 accgcgagcg cgaggtcctt gtcggcgagc gcacccgcat cgtgaaccgg atgaaaggca
     1141 ctctggcccg cctgggcatt cgcggcttca agccggcgct gccccgtgca ccggagcgcc
     1201 tcgagacgct gcgcacgcca gaaggcgcga cattgccgcc aaatacccgg gccgagctgc
     1261 agcgcgatat ggcgcgcctg cgctttgtga cggaccagat cagagagctt gaggcggcgc
     1321 gggccgagcg cctggagcac gcacccgaga caggaccgaa cgcaatgatc cgcttgctgg
     1381 cgcgagtggt cggggtcggg atcgagactg cggacatgct cgtgcaggag gtgctctcgc
     1441 gcaacctgcg cgaccgccgg gctgtggcac gctacgcagg tctgaccggc gcaccggacg
     1501 agagcggggc caagcggcgt gagaaagggc tggccaaggc cggcaacgcg cgggtgcgcc
     1561 ggggcatgat ccagctggcc tggcgtttcc tgctgtttca gaaggacagc gccctgagcc
     1621 aatggtatcg ggcccgcacc acggacgctc gcggagccac gcgcaagacc ctgatcgtgg
     1681 cccttgcgag aaagctgctc atcgctttgt ggcgcttcgg ggcaaccggc gaggtgccgc
     1741 agggtgtcgt cctgcgtccg gccgcgtgac gcacctgctc ggcgtcggct gaagccgacc
     1801 ccttctgtaa gccgtccaga tccgacaaac ggtctgtccg acagacggcc tgcagacccc
     1861 gatccgaggt ggcggtcatc cgatcgcaac catggctatc atgccggatt acagaatgga
     1921 cccgccgccc ggagcttcgc cgtcgatgcg catgactgca tcatggtccg gacccactgc
     1981 gtccgaccga atacaagttc gtggcgcgat gatacgtgct ctatggcgag ctcccctcgg
     2041 cctcgtgatc tgcccaacgc ccgtcgcagt cggtctgccg ccccgagaga aactggaggg
     2101 aaagagagcg ctatcgcgat ttcgcttgac accgcatagc ctcatacaag ttgcgatcta
     2161 cattgatctc tgaacctggg tcaccgtgat ccggcgatca tggatggcgg cgatgacggg
     2221 tggctcggtt gagacgacgc tggagctgtg ggcgtcgtcg ttgcgggacg tcaaggggcg
     2281 catccggccc ttgttccagc aggcgcgcat ggcgcaatcc gcggggatgt tcctggatgc
     2341 cctgctcggg ccggagcggc gcaagacggg ctggatgcga gcggaagccg cgggtgatcc
     2401 cggtccctgg cgccagcagg ccctgctcgg ccgcgcgctg tggaaggcgg atgctctgcg
     2461 cgatctcgtg cgcgattatg cggttgagac cctctccgag ccggatgccg tgctggtcat
     2521 cgacgagacg gggttcctca agcagggccg ggcttcgtgt ggggtcgggc gccaatacac
     2581 gggctcggcg ggcaagatca ccaactctga tgttcggtgt caaaggctgt gaacgcgaca
     2641 gctcgctccc tgcagagatg gggacccacc gagatagagc agttgcgcaa ggccggagac
     2701 agggccgttc agacgacggt tgagcagact ctgatgcgcg ggttggtcac cgcagttccg
     2761 tgtgttcgtc cggggctgcc tcgatctgtc agcgggcgtc gatgacggtc atcggccaca
     2821 cagttcgtac gagggttccc ctccgaggcc ctgcccccgg ccctgcgcaa gcgcagatgt
     2881 cagtccaagt ggatcggacc ctccgcggga ctgaggtggt ttgacttatg cgcgtgccgt
     2941 ccggaacgat gttggaattg attgcggcgc gttctccagc ggggtgaagg tatcggctca
     3001 ggccgcgagg tcaatcggct gatcggccgg cttttcggcg agcgtcggcc acccgtcgac
     3061 ctttctcatt gtgatctggc ccgaggcgag gagcgcccag aacagcatgg cggctgtttc
     3121 cgccgagggc agcacggtct gggttttgat ccgccgcttg aactcctcgt gcaggcgctc
     3181 cactgcattc gtggtccggg ccgacttcca ttggcttggc ggcaggcggg tgaaggtgaa
     3241 cagcttgtcc ccggcctcct ccaggctgtc ggccacagcc tggcacttga gccgccattt
     3301 gcgcaggaac gccttgcgcc gctcggccac ctccgccgcg ctcgcggcgt agatcatgtc
     3361 ggtgtagtcc gccgagatct cctcatgcag ccggtccggg gcatgggcga ggaggttcct
     3421 gtgcttatga acggtgcagc gttgagcggg cacatcgggc cagagcgcgg ccagcgcctt
     3481 ctccaggccg ggcgcgccgt cgacgatcac gaactcgggt gtccgcaggc cacgggccac
     3541 gagatcctcc aggatcgccc gccaggccgc ctcgctctcg ccgcccatgt ttttgaccgc
     3601 cagcagcacc ttctgcccat cctggcgcac gccgagcacg acaagcagcg agatcgcggt
     3661 cgccttgcgg tcgagccgca cccgcaccac cgtgccgtcc aggatgaggc ggatgatcgg
     3721 ctcggccgac aggtcgcgcc gggtccaggc gtcccagtcc gtcttcacct tgcgccagac
     3781 ccggctgacc acgtccttgc cgaccgcccc accgaaaagc gcggcgagcg cccggcggac
     3841 ccggcgcgtg ttggtgccgg acagataggc cccggcgatc agcgcctcgg cccggcgcgt
     3901 caggcgctgg taggccggaa gggaggcatt gcgccattcc ttggtcccgc cctcgggcgt
     3961 ggcaagccgg gcgcggggca cgcaaaccgt cacggcgccg aacgt
//
LOCUS       DATNBV010000485         4768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3773603,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000485 DATNBV010000000
VERSION     DATNBV010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3773603"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VN627_11415"
     CDS             <1..430
                     /locus_tag="VN627_11415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXD03904.1"
                     /translation="LLEAFYDRVSPEDRRFRFFSAAAHIGHEQLAPLVEGDHFRSECY
                     LALTPDGSLAGTAMLACDKPLDTAEVAISVRADWRGKGLGWALLDLMAGEARRRGVRR
                     IIAIEDRENRAAIELEKEKGFVAHGIDGEPTLVLLEKLLR"
     gene            complement(427..825)
                     /locus_tag="VN627_11420"
     CDS             complement(427..825)
                     /locus_tag="VN627_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03905.1"
                     /translation="MHTQPAPLSPAPAASPYRVTRAQRHAVELLARYAWKGLRGTVLL
                     KDLTAQGARIEGLEALRQGDGLTLLLPDAPAVEATVVWAIGRAAGLRFDRAISNEALV
                     RIVRDFATAALEPLKPETLSRRELAPRLCA"
     gene            complement(884..2296)
                     /locus_tag="VN627_11425"
     CDS             complement(884..2296)
                     /locus_tag="VN627_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HXD03906.1"
                     /translation="MTTPATALTAHPWFFCGIGGSGMLPLALILRGQGAAVAGSDRSR
                     DQGRTPEKFAWLESLGFALFPQDGSGIASADQTLVASAAVEDSVPEVVRSKELGCPRM
                     SRAQLLAALFNAAPVSIGIAGTSGKSTVTGMIGWILTQAGRDPTIMNGAVMKNFVAPD
                     APFASARVGSGGVFVSEVDESDGSIALYDPTVAVLGNVSLDHKSLDELRALFGDFLAR
                     SGCAAINLDDADVAALAPRAAHTVSFAIANQAADLGVSGIAEAQTTIAATLHDRRDGT
                     DHALALQVPGRHNLANALAAIAASSAAGVPVTEAVAALASFTGLARRFDIVGTSPSGV
                     TVIDDFGHNPDKVAATLATLKAHPGRVIAFFQPHGYGPLRQMGEELARTFARGLGPDD
                     RLLLCDPVYFGGTVDRSVGSEWIVELVNASGGSAEYLAARADCAARIVALAQPGDRVV
                     IMGARDDTLTLFAKDLLASL"
     gene            2354..3181
                     /locus_tag="VN627_11430"
     CDS             2354..3181
                     /locus_tag="VN627_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HXD03907.1"
                     /translation="MIRVAICAPSTPFTRDDAARVLELGREFPGLELHFHDQCFAVDG
                     HFAGADAVRLEAFLDCANDPAFDAVWFVRGGYGANRIAQDAMARLGPAATDKAFLGYS
                     DGGYLLAALYRARIGRPVHAPMPTDIRRAGGEAAVRRSLGWLAGDRSGEEPSLDGRHP
                     AVAFNLTTLAMLGGTPLMPGLAGHVVMVEEVSEYLYAVDRLFFHAAAHLGGIAGLRLG
                     RVSEVPENDRPFGAGPEQIARFWCERHGLAFLGAADIGHDAANRIVPFGLASNRRDS"
     gene            3218..4156
                     /gene="fabD"
                     /locus_tag="VN627_11435"
     CDS             3218..4156
                     /gene="fabD"
                     /locus_tag="VN627_11435"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HXD03908.1"
                     /translation="MRAFVFPGQGSQKVGMGVELAEASAAAREVFEEVDDALGQKLFQ
                     IMREGPDDALTLTENAQPAIMANAIAVLRVLEKEGGLALADKADFVAGHSLGEYTALC
                     AAGAFSLADTARLLKLRGQAMQAAVPVGVGAMAALLGADIEKASALAEAAAEGQVCTV
                     ANDNDPTQVVISGHREAIERAVALVKDFGIKRGMLLPVSAPFHCPLMQPAADAMAEAL
                     GKTPPGTLRVPVFANVIAAAASEPADVQRLLVEQVTGRVRWRESAIAMRDAGVEQFVE
                     LGGKVLGPMITRSAGPDVAVTSVVTMADIEELAKGL"
     gene            4195..4587
                     /locus_tag="VN627_11440"
     CDS             4195..4587
                     /locus_tag="VN627_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HXD03909.1"
                     /translation="MAEVDEISSAVLNEAIRIHRELGPGLLESVYEQVLAAALLRRGL
                     EVARQVPVEISYEGMVIAGAFRVDLLVGQTLVVEIKSVEQLTKLHAKQLLTYLRLMKQ
                     PVGLLLNFSGLTMKEGIRRLVNDYHETP"
     gene            4639..>4768
                     /locus_tag="VN627_11445"
     CDS             4639..>4768
                     /locus_tag="VN627_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03910.1"
                     /translation="MFDLHGMTALVTGASGGIGSSIARALASQGARLAISGSNATKL"
BASE COUNT          767 a         1577 c         1646 g          778 t
ORIGIN      
        1 cctgctcgaa gccttttacg accgggtcag ccccgaggat cgccgcttcc gcttcttcag
       61 tgccgcagcc catatcggcc acgagcagct ggccccgctg gtggaaggcg atcacttccg
      121 ctcggaatgc tacctcgccc tgacacctga cggcagcctc gccggcacgg ccatgctggc
      181 ctgcgacaag ccgctcgata cggccgaagt ggcaatttct gtccgggccg actggcgtgg
      241 caagggcctg ggttgggccc tgctcgactt gatggcgggc gaggcccggc ggcgcggcgt
      301 gcgccggatc atcgcgatcg aggaccgcga gaatcgcgcg gccatcgagc tggaaaagga
      361 aaaggggttt gtcgcgcacg gcatcgacgg cgaaccgacg ctggtgctgc tggagaagtt
      421 gctgcgttag gcacagagcc tcggcgccag ctcccggcgc gacagggtct ccggtttcag
      481 cggctccagc gctgccgtgg cgaaatcgcg cacgatccgc accagcgcct cgttgctgat
      541 ggcgcgatcg aaccgcagcc cggcggcgcg gccgatggcc cagaccacgg ttgcttccac
      601 cgccggcgca tcgggcagca gcagggtcag cccgtctccc tgccgcagag cctcgaggcc
      661 ttcgatccgg gcaccctgcg cggtcaggtc cttgagcagg actgtgccgc gcaggccctt
      721 ccaggcataa cgggcgagca gttcgaccgc atggcgttgg gcacgggtga ccctgtacgg
      781 ggaagctgcc ggggcggggg acaggggagc cggttgggtg tgcatgttgt ctgcctcgct
      841 cgcgggttaa ccacaggcag caagacggcg acggtcgctc gatttaaagg ctggcgagca
      901 gatctttcgc aaacagggtc agagtatcat cgcgcgcgcc catgatcacc actcggtcac
      961 ccggctgcgc cagcgcgacg atccgggccg cgcaatcggc ccgcgcggca aggtactcgg
     1021 ccgatccgcc ggaggcgtta accagttcca ctatccattc gctgcccacc gacctgtcga
     1081 cagttccgcc gaaatagacc ggatcgcaca gcagcagccg gtcatccgga ccaagccccc
     1141 gcgcgaaggt gcgggccagt tcctcgccca tctggcgcag cggcccatag ccgtggggct
     1201 ggaagaaggc gatcacccgc cccgggtggg ccttcaaggt ggccagggtg gcggcgacct
     1261 tgtcggggtt gtggccgaaa tcgtcgatca cggtcacgcc cgacgggctg gtgccaacga
     1321 tgtcgaaccg ccgcgcaagg ccagtgaaac tcgccagcgc tgccacagcc tcggtcaccg
     1381 gaaccccggc tgcgctgctg gcggcgatgg ctgcgagggc atttgccaaa ttgtgtcggc
     1441 cggggacctg aagggccagc gcatgatccg tgccgtcgcg tcgatcatgg agcgtcgcgg
     1501 cgatggtcgt ctgcgcctcg gcaatgccgc tcacgccaag atcggccgcc tggttggcaa
     1561 tggcgaagct gaccgtatgg gccgcgcgcg gggcgagcgc agccacatcc gcgtcgtcga
     1621 ggttgatcgc cgcgcagccc gaccgcgcga ggaaatcccc gaacagcgcc cgcagttcat
     1681 caaggctctt gtgatcgagg ctgacattgc cgagcacggc caccgtcgga tcgtacaggg
     1741 cgatcgagcc atcgctttca tcgacctcgg agacgaacac cccgcctgag ccgacccggg
     1801 ccgaggcaaa gggcgcatcg ggcgccacga agttcttcat caccgcgcca ttcatgatgg
     1861 tcggatcgcg gcccgcttgg gtcaggatcc agccgatcat tcccgtgacc gtggacttgc
     1921 cgctggtgcc ggcgataccg atcgaaaccg gcgcggcgtt gaacagggcg gcgaggagct
     1981 gggcccggct catgcgcggg cagccgagtt ccttggatcg cacgacttcg ggcacggaat
     2041 cctcgacggc ggcggaggcg accagcgtct ggtccgcgct cgcgatcccg ctgccgtcct
     2101 gggggaacaa ggcgaagccg aggctttcga gccaggcgaa cttttccggc gtgcggccct
     2161 ggtcgcggct gcggtccgac cctgccaccg cggcgccctg gccgcgcagg atcagcgcca
     2221 acggcagcat gcccgagccg ccgatgccgc agaagaacca tgggtgggcg gtgagggcgg
     2281 tggcgggcgt ggtcatcgtg ggccagcggt atgcgtcctt tgcccgggag gcaactgtgg
     2341 ttaggaaggc ggcatgatcc gcgtcgccat atgtgcaccg tccaccccgt tcacccgcga
     2401 cgatgccgcg cgggtgctgg aactggggcg cgaatttcca ggtttggagc tgcacttcca
     2461 tgaccagtgc ttcgccgttg acggccattt tgccggcgcc gacgcggtgc ggctcgaagc
     2521 ctttctcgac tgcgccaacg atcccgcctt cgatgccgtc tggttcgtgc gcggcggcta
     2581 cggggcgaac cggatcgccc aggacgccat ggcgcggctc ggcccggcgg cgacggacaa
     2641 ggcctttctc ggctattcgg acgggggcta cctgctggcc gcgctctacc gggcccgcat
     2701 cgggcgtccg gtccacgcgc caatgccgac ggacatccgc cgcgctggcg gtgaggcggc
     2761 ggtacggcgt tcgctcggct ggcttgccgg tgaccgttct ggcgaggagc cgtcgctcga
     2821 tggccgccac ccggcggttg cgttcaatct cactacgctg gccatgctgg gcgggacgcc
     2881 gttgatgccg ggccttgccg gccacgtggt catggtcgag gaggtctcgg agtatcttta
     2941 cgcggtggac cggctgttct tccacgccgc cgcccacctc ggcggcatcg ccggcctcag
     3001 gctcggccgc gtcagtgaag tgccggagaa cgaccgtcca ttcggcgccg ggccggagca
     3061 gatcgcccgc ttctggtgcg agcgacacgg cttggcattc cttggggcgg ccgacatcgg
     3121 ccatgatgct gccaacagga tcgtgccgtt cgggcttgcc agcaatcggc gcgattccta
     3181 aggccgcgcg caatcaggat taaggagtag cggaacaatg cgcgcttttg tgtttccggg
     3241 gcagggcagc caaaaggtcg gcatgggcgt ggagctcgcc gaagccagcg cggccgcccg
     3301 cgaggtgttc gaggaggtcg acgatgcact cggccagaag ctgttccaga tcatgcgcga
     3361 agggcccgac gatgccctga ccctgaccga aaatgcccaa ccggcgatca tggccaatgc
     3421 catcgccgtc ctgcgcgttc tggagaagga aggcggcctc gcgctcgccg acaaggccga
     3481 tttcgtcgcc ggccatagcc tcggcgagta caccgcgctc tgcgccgccg gggccttttc
     3541 gctggccgac accgcccggc tgctcaaact gcgcgggcag gcgatgcagg cggcggtccc
     3601 ggtcggcgtc ggcgcgatgg cggcgctgct gggggccgat atcgagaagg cgtccgcgct
     3661 ggccgaggca gccgccgagg gccaggtctg caccgtcgcc aacgacaacg accccacgca
     3721 agtcgtcatc tccggccatc gcgaggcgat cgagcgagcg gtggcgctgg tcaaggactt
     3781 tgggatcaag cgcggaatgc tgctcccggt ctcggccccg ttccactgcc cgctgatgca
     3841 gccagccgcc gatgcgatgg ccgaggcgct gggcaagacc ccgccgggca ccttgcgcgt
     3901 cccggtcttc gccaacgtca tcgccgccgc cgccagcgag cccgccgatg tccagcgcct
     3961 gctggtcgaa caggtcaccg gccgggtccg ctggcgcgaa agcgcgatcg ccatgcgcga
     4021 tgccggcgtc gagcagttcg tcgaactggg cggcaaggtc ctcggcccga tgatcacccg
     4081 cagcgccggg cccgacgtgg cggtcaccag cgtggtgaca atggccgata tcgaggaact
     4141 ggctaagggg ctgtaagagg ttcgcgcaga gaccgcaaag gacgcagagg ggggatggcc
     4201 gaagtcgacg agatttcctc agccgtgctc aatgaggcca tccggattca tcgcgagctt
     4261 gggccggggc tgctggaaag cgtttatgag caggtccttg cggcagctct tctacggcgg
     4321 ggactggaag tggcccggca ggtgccggtc gagatcagtt acgagggcat ggtgattgcc
     4381 ggtgccttcc gggtggacct tctcgtaggg caaactctcg tcgttgagat taagtccgtg
     4441 gagcagctga ccaagcttca cgccaagcaa ctgttgacct atctgcgact tatgaagcag
     4501 cccgtcgggc tgcttctgaa tttttccggc ctgacgatga aggaaggcat tcgccgcttg
     4561 gtaaacgact accatgaaac tccttgatac ggcgttctct gcgatctctg cgcgaaccaa
     4621 taatcggagc acacgaccat gttcgacctt cacggcatga ccgccctcgt caccggcgcc
     4681 tcaggcggca tcggctcctc cattgcgcgg gcgttggcct cgcagggggc gcggctggct
     4741 atttcgggct ccaatgccac caagctgc
//
LOCUS       DATNBV010000486         2877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4391984,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000486 DATNBV010000000
VERSION     DATNBV010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4391984"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VN627_11450"
     CDS             complement(<1..1196)
                     /locus_tag="VN627_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXD03911.1"
                     /translation="MTAKTKALPSLRRELVRALSLISVAWLAAVFVAVAIGVRHEVDD
                     LMDGALQEAAEILYGLLAPSSPDRPLMAAGSMLAPPHNEGLVWQIVDERQQVVLRSQK
                     APSAPLLPTFAAGLSDSPLDWRVYAMRLPAAGQALYVGQPGRDRVQSRYKAIAVIGAS
                     ALAVGMLCVAWLRRRMVGAMRPLQELSLQVKHYDPMRAETDLPAPSREEFLDIREAIR
                     DLGQRLAGRIHGERAFSAHAAHALRTPLAGMEAQLAVAMKEASESARPRLERTREAVH
                     RLKRVVASLLALFRSAEELKVEDIDIEELVLLLPVESLVVHVAAGCRVRGDANLLAAA
                     LANLLDNCIRHGARECWVSCRSAENRQTLTLIDDGPGVTDEHRAALQLGLDHATDLDS
                     GGLGLKL"
     gene            complement(1193..1846)
                     /locus_tag="VN627_11455"
     CDS             complement(1193..1846)
                     /locus_tag="VN627_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXD03912.1"
                     /translation="MRLLLVEDDEILGEGIRDYLRIEGHLVDWFTRLIDAQTVISEPY
                     DAVLVDWQLPDGSGVDWVRHLRGKGMTLPILVMTARDLLSDRVKGLDSGADDYLVKPF
                     ETEELAARIRAVRRRVSGHGIPLVRIGSVEIDANARTVSRGGKPVDLTAREWALLDAL
                     MTRSGRVVSKPDLDALLTGFKGEAASNSLEVHVFNLRRKLGRDIVQTVRGVGYRIPL"
     gene            1897..>2877
                     /locus_tag="VN627_11460"
     CDS             1897..>2877
                     /locus_tag="VN627_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXD03913.1"
                     /translation="MGNEYLAIYGALAGAGVALHVFLRARREREAKAAREAASQAGLD
                     EPASLHPVADPARCIASGGCVRACPESALGIVNGLAVLVNASACIGHGACVAACPVEA
                     ITLVYGTERRGVDIPTVSPRFETNVPGIFIAGELGGMGLIRKAAEQGRQAIEAVRTRR
                     AAADELEVLIVGAGPAGISAGLAAKKHGMRYRIIEQEASLGGTVYHYPRNKIVMTAPV
                     KLALVGKVRMTKVSKERLLSFWNDVVRKTGLVLNFGERMVQVDREGGSFVVHTSAGVH
                     RARSLLLAIGRRGSPRTLGVPGEELPKVVYRLVDAQQYRGMSVLVVGGGDR"
BASE COUNT          491 a          956 c          953 g          477 t
ORIGIN      
        1 agcttcagac ccagtccccc ggaatcgaga tccgtggcat ggtccagccc cagctgcagg
       61 gcagcgcggt gctcgtccgt cacgccggga ccgtcgtcga tcagggtcag cgtctgccgg
      121 ttttccgcgg aacgacagct cacccagcac tcgcgggccc catggcggat acagttgtca
      181 agcaggttgg ccagcgccgc cgcaagcaga ttggcatcgc cgcggacccg gcagcccgct
      241 gccacgtgaa cgaccagcga ttccaccggc agcaggagca cgagttcttc gatgtcgatg
      301 tcctcgacct tgagctcctc ggcgctgcga aacagcgcca atagcgaagc gacgacgcgc
      361 ttgagccgat ggacggcctc ccgggtgcgc tccagccgcg ggcgcgcact ctccgaggcc
      421 tccttcatgg cgacggcaag ttgcgcctcc atgcccgcca atggcgtgcg cagcgcatgc
      481 gctgcatgtg cggagaacgc ccgttcgcca tggatgcgcc ccgcaaggcg ttgaccgaga
      541 tcccggatgg cttcccgaat gtccaggaat tcctcccgcg atggcgccgg caggtcggtt
      601 tcggctcgca tcgggtcgta gtgcttgacc tgcagcgaca gctcctgcag cggtctcatg
      661 gccccgacca tgcggcgcct caaccacgcc acgcaaagca ttccgaccgc cagggcgcta
      721 gccccgatga ccgcgattgc cttgtagcgg gactgcacac gatcgcggcc aggctgtccg
      781 acatacaacg cctgccccgc cgcgggcagg cgcatggcat agacccgcca atcgagcggg
      841 ctgtcgctca ggccggccgc aaacgtcggc agcagcgggg ccgacggggc cttttgggaa
      901 cgcagcacga cctgctggcg ttcgtccacg atttgccata ccaggccctc gttgtgcggc
      961 ggtgccaaca tgctgcctgc cgccatcaac ggccggtcag ggctgctcgg ggccagcagg
     1021 ccatagagaa tctcggcggc ctcctgcagg gcgccgtcca tgaggtcatc cacttcgtgg
     1081 cgcacgccga tagcgacagc gacgaacacg gcggccagcc aggccacgct gatcaggctc
     1141 agcgccctca ccagttcccg gcgcaatgat ggcagggctt tggtctttgc agtcataacg
     1201 gaatgcggta tccgacgccc cgcacggtct gcacgatatc ccgcccgagc ttgcgccgta
     1261 aattgaagac gtggacctcc agcgaattgc tggccgcttc gcccttgaag ccggtcagca
     1321 gcgcatccag gtcgggcttg gacaccaccc ggccggatcg ggtcatcaat gcgtcgagca
     1381 gcgcccattc gcgcgccgtg agatcgaccg gcttgccgcc acgggagacc gttcttgcat
     1441 tggcgtcgat ctcgacggaa ccgattcgaa cgagcggaat tccgtggccc gacacccgcc
     1501 ggcgcacggc gcgaatgcgg gccgccagtt cctcggtctc gaacggcttg accaggtaat
     1561 cgtcagcccc gctgtccagg cccttgacgc ggtcggagag caagtcgcgc gctgtcatca
     1621 ccaggatggg aagggtcatg cctttgccgc gcaggtgcct gacccaatcg actcccgatc
     1681 catcgggcaa ctgccaatcc accagcactg catcgtaggg ctcgctgatg acggtctggg
     1741 catcgatcag ccgggtgaac cagtccacca gatggccttc aatgcgaagg taatcccgaa
     1801 tgccttcacc caggatttcg tcgtcttcta ccagcagcag ccgcattttt cgaattcttt
     1861 ccggatggcc catgcttata gcaggagaat tctggaatgg gcaacgagta cctcgcgatc
     1921 tacggcgcgt tggccggcgc cggcgtggca ctgcatgtgt tcctgcgggc tcgccgcgag
     1981 cgcgaggcga aggcagcgcg cgaggccgca agccaggccg gcctggacga acctgcatcg
     2041 ctgcatcccg tggcagaccc ggcccgctgc atcgcttcgg gcggctgcgt gcgggcctgt
     2101 cccgagtcgg cgctgggcat cgtcaatggc ctcgccgtgc tggtcaatgc ctcagcctgt
     2161 attggccatg gtgcctgcgt ggcggcgtgc ccggtcgagg cgatcacgct cgtgtacggc
     2221 accgagcggc gaggcgtgga cattccgacg gtctcgcccc ggttcgaaac caatgtgccg
     2281 ggaatcttca tcgcggggga actgggcggc atgggcctga tccgcaaggc cgcggagcaa
     2341 ggccggcagg ccatcgaagc agtgcgcacg cgccgcgcgg ccgccgacga gctggaagtt
     2401 ctcatcgttg gcgccggtcc ggcaggcatt tcggccgggc tggcggcgaa gaagcacggg
     2461 atgcgctatc gaatcatcga gcaggaggct tcgctgggcg gcacggtcta ccactatccg
     2521 cgcaacaaga tcgtgatgac cgcgccggtg aagctggctc tggtgggcaa ggtccgaatg
     2581 acgaaggtat ccaaggagcg gctgttgtcg ttctggaacg acgtggtcag gaagacgggg
     2641 ctggtgctga acttcgggga acgcatggtg caagtggatc gcgaaggggg aagtttcgtc
     2701 gtgcacacca gtgccggcgt ccaccgcgcg cgatcgctgc tgctggctat cggccgccgc
     2761 ggctcgccgc gcaccctggg cgtgcccggc gaggaactgc ccaaagtggt ctaccgcctg
     2821 gtcgacgccc agcagtatcg gggcatgtcg gtgctggtgg taggcggcgg cgacaga
//
LOCUS       DATNBV010000487         5105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1262926,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000487 DATNBV010000000
VERSION     DATNBV010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262926"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1894
                     /gene="fdhF"
                     /locus_tag="VN627_11465"
     CDS             <1..1894
                     /gene="fdhF"
                     /locus_tag="VN627_11465"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HXD03914.1"
                     /translation="RYTADRLNAIKAQYGARALGGITSSRCTNEEAYLVQKLVRAGFG
                     SNNVDTCARVCHSPTGYGLKTTFGTSAGTQDFDSVMDSDVILVIGANPTDAHPVFASQ
                     MKRRLRQGAKLIVIDPRRIDLVRSPHVQAEHHLALQPGTNVAVLTAMAHVIVTEGLAD
                     EAYIRERCDWDEYQDWARFVADERHSPEVLQAVTRVPAADLRAAARLYATGGHAAIYY
                     GLGVTEHSQGSSTVMAIANLAMVTGNVGRRGVGVNPLRGQNNVQGSCDMGSFPHELSG
                     YRHISDGETRALFEADWGVALDPEPGLRIPNMLDAAVEGSFRALYVQGEDILQSDPNT
                     HHVAAGLAAMDLVIVHDLFLNETANYAHVFLPGSTFLEKNGTFTNAERRIQLVRKVME
                     PANGYEDWEVTQELAKAMGLGWDYAHPAEIMAEIARLTPSFAGVSHERLDREGSLQWP
                     VNEAHPDGSPIMHVDGFVRGKGKFVVTDYVPTDEKTGPRFPLLLTTGRILSQYNVGAQ
                     TRRTANTVWHEEDVLEIHPTDAENRGLVTGDIVRLASRVGDTTLRALVTDRVAPGVVY
                     TTFHHPDTQANVVTTEYSDWATNCPEYKVTAVQVSPSNGPSDWQKDYTAQAAHSRRIV
                     AAEPAE"
     gene            1891..2085
                     /locus_tag="VN627_11470"
     CDS             1891..2085
                     /locus_tag="VN627_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HXD03915.1"
                     /translation="MSNLVYMANQIARNLAPQGEATAVDQTAQHIRDFWDPRMKAAIL
                     ANGEGLDPLAKAAVEQLRGG"
     gene            2295..4334
                     /locus_tag="VN627_11475"
     CDS             2295..4334
                     /locus_tag="VN627_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXD03916.1"
                     /translation="MEAIGAGARAIAIVGPAGAGKTTLLEALLHAAGAINRSGSVEAG
                     TSLGDASPEARARGGSTELNLARLTWGGDTYTLLDCPGAPGLAAEGDCALAVADLALV
                     VVPPEPERAGLAEPLLRDLAARGIPHLIFVNRIDKARGDSGTSVSALLDALQPLSAAP
                     LVARQMPILEGGAVTGYVDLALDQAFHYREGGPAAPIPLDDSLRAEEQGARTHFLEQL
                     ADHDDNLLERLLNDEEPDLTLIANDLAAETARGEIVPVLFGSATHGFGLRRLLKALRH
                     DTPAPALTARRLGLGGPALEVFKVLGESQVGRLAVGRIFGGMLAEGAELRTADGKALR
                     AGSLFAVQGSAANRLAKAGEGDIVALAKAEGITAGMRLGIGASVAAPDPRPRRTAHHA
                     LAIAVPDHADEVRLSGALGKLVEEDLGLAWETLAETRETLLRGLDEEHVALAIERLRR
                     RHGLKLATRTPTLPLRETVRKSASQRGRHKKQSGGHGQFGDVLIELRPLGRGEGFLFE
                     DRISGGVVPRQWIPAVEQGIRDAMARGPLGFPVVDVAVALVDGSYHSVDSSELAFRTA
                     GRLAMSAALAAAAPYLLEPVAEVEIHAPPGAASRITSTLASRRARVLGMVPRDGWHQW
                     ETIAALVPEAELDRLQSDLRAASQGLASFTARFDHLAEVAGKTADQLIERAKEAA"
     gene            complement(4441..>5105)
                     /locus_tag="VN627_11480"
     CDS             complement(4441..>5105)
                     /locus_tag="VN627_11480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXD03917.1"
                     /translation="QGGRRNEDPVHPPEHALAGGRVLTAADEVVTYVARNLEPYRGYP
                     SFIRALPELLAQRPQAQVVIVGGDEVSYGRSPPEAANWREHMAREVSLDPARVHFTGK
                     LPYAQYLALLQVSALHIYLTVPFVLSWSCLEAMSAGCLILASDTAPVVEAITDRSNGL
                     LCDFHDPADIAGKAAAALAARGDMAGMRKAARQTVLERYDLSRCLPAQMALVSEVAGM
                     RG"
BASE COUNT          848 a         1884 c         1621 g          752 t
ORIGIN      
        1 gcgctacacc gccgacagac tcaacgcgat caaggcgcaa tatggcgccc gcgcgctggg
       61 cggcatcacc tccagtcgct gcaccaacga agaagcctac ctcgtccaga agctggtccg
      121 cgccgggttc ggctcgaaca atgtcgatac ctgcgcccgg gtctgccact cgcccaccgg
      181 ctatggcctg aagacgacct tcggcacctc ggccggcacg caggatttcg attcggtcat
      241 ggattccgac gtgatcctcg tgatcggcgc caatcccacc gatgcgcatc cggtgttcgc
      301 aagccagatg aagcgccgcc tgcgccaagg cgcgaagctg atcgtgatcg atccgcgccg
      361 gatcgacctc gtccgctcgc cgcatgtgca ggcggagcat cacctcgcgc tccagcccgg
      421 taccaatgtc gcggtgctga cggccatggc ccacgtgatc gtcaccgaag ggctggctga
      481 cgaggcctac atccgcgaac ggtgcgactg ggacgaatat caggactggg cccgcttcgt
      541 ggcggatgag cgccacagcc cggaagtgct ccaggcggtc acccgggtcc ccgccgccga
      601 cctgcgcgcc gccgcccggc tctatgccac gggcgggcac gcggcgatct actatggcct
      661 cggcgttacc gagcacagcc agggctcctc cacggtgatg gcgatcgcca accttgccat
      721 ggtgaccggc aatgtcggcc ggcgcggcgt cggcgtgaac ccgctgcgcg gccagaacaa
      781 cgtgcagggt tcgtgcgaca tgggctcgtt cccgcacgaa ctctcgggct accgccacat
      841 ctccgatggc gaaacccgcg ccctgttcga ggcggactgg ggcgtggcgc ttgaccccga
      901 gccgggcctg cgcatcccca acatgctcga tgccgcggtc gagggctcct tccgcgcact
      961 ctacgtgcag ggggaggaca ttctccagtc cgatcccaat acccaccacg tcgccgccgg
     1021 cctcgccgcg atggacctcg tcatcgtcca cgacctgttc ctcaacgaga ccgcgaacta
     1081 cgcccacgtc ttcctccccg gctccacctt cctggagaag aacggcacct tcaccaatgc
     1141 cgagcgccgc atccagctgg tgcgcaaggt gatggaaccg gccaacggct acgaggactg
     1201 ggaggtgacc caggaactcg ccaaggcgat gggcctgggc tgggactatg cccaccccgc
     1261 cgagatcatg gccgagatcg cccgcctgac gccgagcttc gccggggtca gccacgaacg
     1321 gctcgaccgc gagggttcgc tgcaatggcc ggtcaacgaa gcccaccccg acggcagccc
     1381 gatcatgcac gtcgatggct tcgtgcgcgg caagggcaag ttcgtcgtca ccgactacgt
     1441 gccgaccgac gagaagaccg gcccgcgctt ccccttgctg ctcaccaccg gccgcatcct
     1501 cagccagtac aatgtcggcg cgcagacccg gcggacggcc aacaccgtct ggcatgagga
     1561 ggacgtcctc gaaatccacc cgaccgatgc ggagaaccgc ggcctggtca ccggcgacat
     1621 cgtccgcctc gccagccgcg tgggcgacac caccctgcgt gccctcgtta ccgaccgggt
     1681 cgcaccgggg gtggtctata ccaccttcca ccaccccgac acccaggcca acgtggtgac
     1741 gaccgagtac tccgattggg cgaccaactg ccctgagtac aaggtcaccg ccgtccaggt
     1801 cagccccagc aacgggccga gcgattggca gaaggactat accgcccagg ccgctcactc
     1861 gcgccggatc gtcgcggcgg agccggcgga atgagcaatc tcgtctacat ggccaaccag
     1921 atcgcccgca acctcgcccc ccagggcgag gccacggccg tggaccagac cgcccagcac
     1981 attcgcgact tctgggaccc gcgcatgaag gccgcgatcc tcgccaatgg cgaaggcctc
     2041 gatccgctgg ccaaggcggc ggtggagcaa ctgcggggcg gctgacgtcg tcccggacct
     2101 gatccgggac cggtgtcgcg atcttctggc gaggccttgc ccgcaggcga ggctacagct
     2161 ccgcgccgac cagcggtccc gggtcaagcc cgggacgacg cgcattcaaa tcgactcgtt
     2221 gcaatcgcaa ccggattgac cgatgctccc agcgggatcg ccttggacgc cggagggcta
     2281 acgaggagaa gatcatggag gccatcggtg ccggcgctcg cgccatcgcc atcgtgggtc
     2341 ctgccggggc aggcaaaacc acgctgttgg aagccctgct ccacgccgct ggcgcgatca
     2401 atcgcagcgg ctcggtcgag gccggaacgt cgcttggcga tgccagcccc gaagcccgcg
     2461 cacgcggcgg cagcaccgaa ctcaacctcg cgcgcctgac ctggggtggg gacacttaca
     2521 ccctgcttga ttgccccggc gcacccgggc tcgctgcgga gggagattgc gcccttgccg
     2581 tggccgacct cgccctggtc gtcgtcccgc ccgaaccgga gcgggcgggc cttgccgaac
     2641 cgctcctgcg cgatcttgcc gcgcgcggca ttccgcacct catcttcgtc aaccgcatcg
     2701 acaaggcgcg aggcgattcg ggaacatccg tctccgccct gctcgatgcc ctccagcctc
     2761 tcagcgcggc gccgctcgtc gcccggcaga tgcccattct ggaaggggga gcggtcaccg
     2821 gctatgtcga cctcgccctc gaccaggcct ttcattaccg cgaaggcggc cccgccgcgc
     2881 cgatcccgct cgacgatagc ttgcgggccg aagagcaggg cgcccgcacc catttcctcg
     2941 aacaactcgc cgatcacgac gacaacctgc tcgaacgcct gctcaacgac gaggagcccg
     3001 atctcaccct gattgcgaac gacctggccg ccgaaacagc acggggcgag atcgtccccg
     3061 tgctgttcgg ctcggccacc cacggctttg gtctgcgccg cctgctcaag gccctgcgcc
     3121 acgatacgcc cgccccggcg ctcaccgcca gacgtcttgg ccttggcggt cccgcgcttg
     3181 aggtgttcaa ggttcttggc gaaagccagg tgggccgcct cgccgttggc cggatcttcg
     3241 gcggcatgct cgcggaaggt gccgagctca ggactgccga cggcaaggcc ctgcgcgccg
     3301 gctcgctctt cgccgttcag ggcagcgccg ccaaccggct cgccaaggcg ggggaggggg
     3361 atatcgttgc ccttgccaag gccgagggga tcaccgccgg catgcgtctc gggatcggcg
     3421 cgagcgtggc cgcgcccgat ccgcgcccgc gccgaacggc ccatcatgcc cttgccatcg
     3481 ccgtacccga ccacgccgac gaggtccgcc tgtccggcgc gctgggcaag ctggtagagg
     3541 aagacctcgg cctcgcctgg gagacccttg ccgaaacccg cgagaccctg ctgcgcgggc
     3601 tggacgagga gcatgtcgcc ctcgccatcg aacgcctgcg ccgccggcat ggcctaaagc
     3661 tcgccacccg cacgcccacg ctgcccttgc gcgaaaccgt ccgcaagagc gccagccagc
     3721 gcggacggca taaaaagcag tccggcggcc acggccagtt cggggatgtc ctgatcgagc
     3781 tgcgcccgtt gggaaggggg gagggcttcc tcttcgagga ccggatcagc ggcggcgtcg
     3841 tcccgcgtca gtggatcccg gcggtggagc agggcatccg cgacgccatg gccagggggc
     3901 cgctcggctt cccggtggtc gacgttgccg tggcgctggt cgatggctcc tatcacagcg
     3961 tcgacagctc cgaactcgcg tttcgcacgg ccggccgcct cgccatgtcc gcggcccttg
     4021 ccgccgcggc gccctacctg ctcgaacctg tcgccgaggt tgaaatccac gcgccccccg
     4081 gcgctgcctc gcgcatcacc tcgacccttg ccagccgccg cgcccgcgtc ctcggcatgg
     4141 ttccgcgcga tggctggcat cagtgggaga cgattgccgc actggtgccc gaggccgagt
     4201 tggaccgcct ccagtccgac ctgcgcgccg ccagtcaggg gttggccagc ttcaccgcaa
     4261 ggttcgatca cctggcggaa gtggcgggaa aaacggccga tcagctgatc gaacgggcga
     4321 aggaggcggc ctgagagcac cacctgacgg ttgccaaaaa accgttcatg tcgagcgaag
     4381 tcgagacacg aaacgtgcct cagcatcgct cgacacgaac cgggcatggc ctagtttctg
     4441 ttacccccgc atccccgcca cttcgctcac tagcgccatc tgcgccggca ggcagcgcga
     4501 caggtcgtac cgctccagca ccgtctgccg cgccgccttg cgcatgccgg ccatgtctcc
     4561 ccgcgccgcc agcgccgccg ccgccttccc ggcaatgtcc gcggggtcat ggaaatcgca
     4621 cagcaacccg ttcgagcgat ccgtgatggc ctcaaccacc ggcgccgtat ccgatgccag
     4681 gatcaggcac ccggcgctca tcgcctcgag gcacgaccac gagagcacga aggggacggt
     4741 gagataaatg tgcagcgccg aaacctgcag cagcgccaga tactgcgcat agggcagctt
     4801 gccggtgaag tgcacccgcg ccggatcgag gctgacctcg cgcgccatat gctcgcgcca
     4861 gttggctgcc tcgggcgggc tgcgtccgta ggacacctcg tccccgccga cgatcaccac
     4921 ctgcgcctgc ggccgctgcg ccagcagttc gggcagggcg cggatgaagc tgggatagcc
     4981 gcgatagggc tccagattgc gggcgacata ggtcaccact tcgtccgccg cggtcagcac
     5041 ccggccgccg gccagggcat gttctggtgg atgaacagga tcttcattcc gccggccccc
     5101 ctgcc
//
LOCUS       DATNBV010000488         7666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1562956,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000488 DATNBV010000000
VERSION     DATNBV010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562956"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VN627_11485"
     CDS             complement(<1..687)
                     /locus_tag="VN627_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03918.1"
                     /translation="MTTAYRNRLSVSALAAASSFIALSAPVQAQSVCSSVGTLVTCTN
                     GTATTTIDADAVINAAAQGLSITDPTSLSAAIGGTINTTSTLPGLQVQSVGDLTLTAS
                     SGGALSIVGTGGGVGADLSSTTGATTATFGNVTVAGPFAVRAAGQTGTTLTTGSITDT
                     SVSTPPLATVGSAITAYSFTGPATLVVNGNVVAAGAGNVVIGAVANASVGAAAATVNG
                     SVTTTATAGSG"
     gene            1052..1288
                     /gene="purS"
                     /locus_tag="VN627_11490"
     CDS             1052..1288
                     /gene="purS"
                     /locus_tag="VN627_11490"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HXD03919.1"
                     /translation="MKVRVHVSLKNGVLDPQGRAIQHAIEGLGFAGVKDVRAGRLFEL
                     EVDPGVTDAQLEEMASKLLANMVIENYRIERVAA"
     gene            1288..1968
                     /gene="purQ"
                     /locus_tag="VN627_11495"
     CDS             1288..1968
                     /gene="purQ"
                     /locus_tag="VN627_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HXD03920.1"
                     /translation="MPFRSAVITFPGSNCDRDMAVALEQVSGTAPHRVWHGDSELPAG
                     LDFIAVPGGFSYGDYLRSGAMAARSPIMQAVIAAAGRGVPVLGVCNGFQILTEAGLLP
                     GALMRNAGIRFVCKEVALRVENSRSLFTSAYSAGQVIRIPVAHHDGNYFADQATLDRL
                     EGEGRVAFRYAEECNGSARDIAGVLNEAGNVLGMMPHPERAIEAAHGSTDGRALFEAA
                     IKGLVESL"
     gene            complement(2100..4466)
                     /locus_tag="VN627_11500"
     CDS             complement(2100..4466)
                     /locus_tag="VN627_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXD03921.1"
                     /translation="MKTRPPEIATLPKDLPILAVLGLTQPPEGDWGRLRGLQYASLAR
                     VTVARIIAHGIAAIATVQIFVGSIWFLVLIGWFVALCGALYYGARYDRSLGDADRRRM
                     SREEVNTQTISSTIVALVWTVPILFFARYGPSDARLQLWTIVAMLMTATAVVVPAVPL
                     GTILFTAIVGGISVLQFGLAGMWQTAIIALVFMIIVSVGTVESARTYLASRVAEAGML
                     EKSEVVSLLLREFEEGEADWLWQVDTSRRVRSVSPRFAFALGLDPEDIEGQSFIQLIA
                     GEAWETGQFPSSLHDLAERLKRRESFSNVLVRVTIKGRQRWWELSGTPKLDENGVYEG
                     FRGVGSDVTEQRESADKIAQLARYDTLTGLPNRLMLTEAMGEAMRYAEQWRTRCAFLM
                     IDLDRFKAVNDTLGHVVGDQLLARVSARLKELMSENELCGRLGGDEFAIVIRDASDLA
                     RVDLVAKAVIERLSQPYEVDHHTLYVGASVGSAIGPRDGSTVETLMRNADLALYRSKD
                     AGGGKHFTYEPALHAHAEERRKLEFSLRRALERNELLLHYQPVVSADSETVVSFEALL
                     RWNSADHGFVSPAKFIPLAEDTRLIVPIGEWVLREACAEAMRWPRHIKVAVNVSGEQL
                     LDPQFATTVVSALAVTGLPAPRLEIEVTESIFLRDATAARNALEQLMALGCSIALDDF
                     GTGYSSLGYLRKLRFSTIKIDRSFVQGAAQKNPESLAIIRAVVAMAESLDVTTTAEGV
                     ETETELQVVKSLGCNKIQGFFFGRPMPATDARALFVPGMDQRAAGQAA"
     gene            4742..5596
                     /locus_tag="VN627_11505"
     CDS             4742..5596
                     /locus_tag="VN627_11505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXD03922.1"
                     /translation="MPLPRITIVTPSFRAEQFIQTTIESVLGQGYPDLEYIILDGAGD
                     DTASILQRYDDQLAFWRSAPDAGQYAAINEGFARATGEILCWLNADDMLLPRALFVVG
                     EIFAQHPGLEWVSTIHPAIFDANANLAKVGRLPGFNKQAFLDGLFLPGTQDKGFWIQQ
                     ESTFFRRSLWQRAGGRIPDYPLAGDFALWCEFYKHADLVAVEYPLSGFRMLEGQRSGD
                     LEQYLREGAQALQRLREHEGHPARRVACKTDFYPGRIVMNTARNRIGAPWHIVDHEFA
                     AETDLPLW"
     gene            complement(5615..7438)
                     /gene="glmS"
                     /locus_tag="VN627_11510"
     CDS             complement(5615..7438)
                     /gene="glmS"
                     /locus_tag="VN627_11510"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXD03923.1"
                     /translation="MCGIIGIVGKDEVADRLVDGLRRMEYRGYDSAGVCTVHGGELIR
                     RRAPGKLQNLVVELARHPAPGTTGIAHTRWATHGAPTAANAHPHGTEHLALVHNGIIE
                     NFRPLREALIARGRHFESDTDTEVVAHLLSVQVEAGKSPEAAVQAVLPELRGAFALAI
                     AFKAHPDMLIGARLGSPLVVGYGDGETYLGSDALALAPLTQRISYLDEGDWVVITREG
                     AQVYDAANRPVTRPIVASGASAAAIEKGNYRHFMQKEIFEQPTVVAQTLRSYVRQVDQ
                     SVALPQLDFDLSQINRVTIVACGTSYYAGLVAKYWFEQFARLPVDIDVASEFRYRDPV
                     LEPGGLALFISQSGETADTLAALRHCKAAGQTIAVVVNVPTSSMAREADLLLPTHAGP
                     EIGVASTKAFTCQLAVLAALAAHLAVKRGRMSREEEQDVVAQLIETPSALNAALLHDD
                     EIAAMAHLIAPARDVLYLGRGQDYPLALEGALKLKEISYIHAEGYAAGEMKHGPIALI
                     DEAVPVIVLAPSGPLFEKTVSNMQEVRARGGKIVLISDAEGLAEAGEGCLATIEMPKV
                     HPLIAPLVYAVPVQLLAYHVACAKGTDVDQPRNLAKSVTVE"
     gene            complement(7460..>7666)
                     /locus_tag="VN627_11515"
     CDS             complement(7460..>7666)
                     /locus_tag="VN627_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXD03924.1"
                     /translation="ERFLANFAIREVTPEIARRAAGLKAERPGLSLPAALVFASAQEH
                     GAILVTRNSAEFPANIPGIRIPYT"
BASE COUNT         1314 a         2591 c         2484 g         1277 t
ORIGIN      
        1 gccacttccc gccgtagcag tggtggttac ggacccattc accgtggctg ctgcagcgcc
       61 aacggacgcg ttcgcgaccg cgccgatcac gacgtttcca gcaccagcag cgactacgtt
      121 gccgttcaca accaatgtag cagggccggt aaatgagtac gctgtgatgg ccgagcccac
      181 agtcgccagc ggcggcgttg aaacactggt gtcagtgatg gatccggtag tcagtgtcgt
      241 gccggtctgg ccggctgccc gaacggcaaa cggcccggcg acagtgacat taccaaaagt
      301 tgccgtggtc gcaccagtgg tggaactcag atcggcgcca acgccacccc cagttcctac
      361 gattgaaagt gcccctccgg aagaagccgt aagcgttaga tcgccaacgg attgcacttg
      421 caagcctgga agcgtcgaag tggtgttgat cgttccgccg attgcagcgc tcagagaagt
      481 cggatcggta atagaaaggc cttgagcggc tgcattgatc actgcgtctg cgtcgatggt
      541 cgtcgtcgcg gttccattag tgcaggttac caatgtgccc acggaggagc agacgctctg
      601 cgcctggacc ggcgccgaca gcgcgataaa cgaacttgcg gccgccaagg ccgaaacgga
      661 caagcggttt cgataggccg tcgtcataga attgtccctc attgctggcg catccgccct
      721 gccgcgctgt acggcacgta cggcgggttt attttgtgcg cagacagggg ttcccccgcc
      781 cgcacacata acttgggata ggttcggcgg ttccccggtc aacggatttt tttatttaaa
      841 tccattgtct tgcggtgaat cgttagtaaa cacaccggta agtctaaagt agctgggtgc
      901 gccaattggg cacgccagga gcacccctcc tgactgtgcg aaaacccccg caagggcctt
      961 gcccaaggcc cgcttgccct tgccccgcgc ggggttcgcg ggcataggcg ccggatccgt
     1021 cagccgccat ttgcccacga aaggctcgtc catgaaggtc cgcgtccacg tcagcctcaa
     1081 gaacggcgtt ctcgatccgc aggggcgggc aatccagcat gcgattgagg gcctgggctt
     1141 cgccggggtg aaggacgtgc gggccggccg cctgttcgag ctggaggttg accctggcgt
     1201 gaccgatgcc cagcttgaag aaatggccag caagctgctc gccaacatgg tcatcgagaa
     1261 ctaccgcatc gaaagggtcg ccgcctgatg ccgttccgct ccgccgtcat caccttcccc
     1321 ggctccaact gcgaccggga catggccgtg gccctcgagc aggtctcggg cacggcgccg
     1381 caccgcgtct ggcatggtga cagcgagctg cccgcaggcc tcgatttcat cgccgttccg
     1441 ggcggcttct cttatgggga ctacctgcgc tcgggcgcca tggccgcgcg ctcgccgatc
     1501 atgcaggcgg tgatcgccgc ggccggccgg ggcgtgcccg tgctcggcgt gtgcaacggc
     1561 ttccagatcc tcaccgaagc cggcctgctg ccgggtgcgc tgatgcgcaa cgccggcatc
     1621 cgcttcgtct gcaaggaagt ggccctgcgc gtggaaaaca gccggagcct gttcaccagc
     1681 gcctattccg ccggccaggt catccgcatc cccgtcgccc atcacgacgg caactacttt
     1741 gccgaccagg ccacgctgga ccgcctcgaa ggcgaaggcc gcgtcgcctt tcgctatgcc
     1801 gaggagtgca acggctcggc ccgcgacatc gccggcgtcc tgaacgaagc cggcaatgtg
     1861 ctgggcatga tgccccaccc cgaacgcgcg atcgaggcgg cccatggctc cacggacggc
     1921 cgcgccctgt tcgaggccgc gatcaagggc ttggtcgaga gtctttgatt ggtgcgggtc
     1981 gggcctccgc ccgacccttg ccgcaccagc cacccattga gtgtaccttg tgggtggtcg
     2041 ggccggggga gcgggctggt gccgaaagcc ttccacggat gtggagggcg cacttcaaac
     2101 tacgctgcct gccccgcggc ccgctggtcc atgcccggca cgaacaaggc acgggcatcc
     2161 gtggccggca tcgggcgtcc gaagaagaag ccctggatct tgttgcagcc cagcgacttg
     2221 acgacctgaa gctcggtctc cgtctccacg ccctcggccg tggtggtgac gtcgaggctc
     2281 tcggccatgg cgacgacggc gcggatgata gccaggctct ccgggttctt ctgcgccgcc
     2341 ccctggacga aggaacggtc gatcttgatc gtggagaacc tgagcttgcg cagataaccc
     2401 aatgaagaat aaccggtccc gaagtcgtcg agcgcgattg agcagcccag ggccatcagc
     2461 tgttccagcg cgttgcgggc cgctgtcgca tcgcgcagga agatcgattc ggtcacctcg
     2521 atctccagcc gcggcgccgg caggccggtg acggccagcg ccgagacgac ggtcgtggca
     2581 aactgcggat cgagcagctg ctcgcccgaa acgttcaccg ccaccttgat gtggcgcggc
     2641 caccgcatcg cttccgcgca ggcctcgcgc aagacccact cgccgatggg cacgatcagg
     2701 cgcgtatcct ccgccagcgg aatgaacttg gccggggaaa cgaagccatg gtccgcgctg
     2761 ttccagcgca gcagggcctc gaacgagacc acggtctcgc tgtcggcgct gaccaccggc
     2821 tggtagtgca gcaacagttc gttgcgctcc agcgcgcggc gcagcgagaa ttcgagcttg
     2881 cggcgctcct cggcgtgggc atgcagcgcc ggctcatagg tgaagtgctt gccgcccccg
     2941 gcatccttgg agcgatagag cgcaaggtcg gcgttgcgca tcagtgtctc gacggtcgat
     3001 ccgtcgcgcg ggccgatcgc ggagccgacg ctggcgccca cgtagagtgt atggtgatcc
     3061 acctcatagg gctgcgacag ccgctcgatc accgccttgg cgacaaggtc cacccgggcg
     3121 aggtccgagg catcgcggat gacgatggcg aattcgtcgc cgccgagccg cccgcacagc
     3181 tcgttctcgc tcatcagctc cttgagccgc gcggaaaccc gcgccagcag ctggtcgccg
     3241 acaacgtggc cgagcgtatc gttgaccgcc ttgaaccggt cgaggtcgat catcaggaaa
     3301 gcgcagcgcg tgcgccactg ctcggcatag cgcatggcct cgcccatcgc ctcggtcagc
     3361 atcaggcggt tgggcaggcc agtcagcgta tcgtagcggg cgagctgggc gatcttgtcg
     3421 gcgctctcgc gctgttcggt cacgtccgaa cccacgccgc gaaagccctc gtagaccccg
     3481 ttctcgtcca gcttgggggt gccggacagc tcccaccacc gctggcggcc cttgatggtg
     3541 acgcgcacca gcacgttgga gaaggactcg cggcgcttca gccgctcggc caggtcgtgc
     3601 agcgacgagg ggaactggcc ggtttcccac gcctcgcccg caatcagctg gatgaacgac
     3661 tggccctcga tatcctcggg gtcgagtccc agcgcaaagg cgaagcgcgg gctgaccgag
     3721 cggacgcggc gcgacgtgtc gacctgccac agccagtcag cctcgccctc ctcgaactcg
     3781 cgcaggagca gcgagacgac ctcgctcttt tcgagcatgc cggcctcggc cacgcggctc
     3841 gccagatagg tccgcgcgct ttccaccgtg ccgaccgaga cgatgatcat gaacactagg
     3901 gcgatgatcg ccgtctgcca catgccggcc aggccgaact gcagcaccga tatccccccc
     3961 acgatggcgg tgaacaggat cgtgccgagc ggcaccgccg ggacgacgac ggccgtcgcc
     4021 gtcatcagca tcgccacgat ggtccacagc tgcagccgcg cgtccgatgg gccgtagcgt
     4081 gcaaagaaca ggatcggcac ggtccagacc agggccacga tggtcgaact gatggtctgg
     4141 gtgttcactt cctcgcggct catccgccgc cggtcggcat cgcccagcga acggtcatag
     4201 cgcgcgccgt aatagagcgc gccgcaaagc gcgacgaacc agccgatcag caccagaaac
     4261 cagatcgagc ccacaaagat ctgcaccgtg gcgatcgccg cgatgccatg ggcgatgatt
     4321 cgggcgacgg tgacgcgggc gaggctggca tattgcagtc cgcggaggcg cccccagtca
     4381 ccttcgggcg gctgggtcag cccgagcacc gcgaggatcg gcagatcctt gggcagcgtg
     4441 gctatttccg gcggacgcgt tttcacgaga ctggcctacg ccagcattcc ttagcaccgc
     4501 gtaaacttgc agttgccccg cggtgccggc tcggctcgat tatggcgtat gatcaacgag
     4561 ttttaaccga gccgtcgcgc gatccaggca attgccccca ggttgacgca aggatcgccg
     4621 cgaaagcgga gggttctgga actcgacagg ggatgcggcc gaggctagtc ccgccgggat
     4681 tacggtaaat ccgccgccga tttgccggat aatactcctc ctggaacgac ggatcattcc
     4741 cttgcctctg ccccggatca cgatcgtcac cccatcgttc cgcgccgaac agttcatcca
     4801 gacgacgatc gagtccgtgc tcggccaagg ctatccggat ctcgaataca tcattctcga
     4861 tggcgcggga gacgatacgg ccagcatcct gcagcggtat gacgaccagc tggccttctg
     4921 gcgcagcgcg cccgatgccg gccaatatgc cgcgatcaac gaaggcttcg cccgcgccac
     4981 gggtgagatc ctgtgctggc tcaatgccga cgacatgctg ctgccgcgcg ccctgttcgt
     5041 ggttggcgag atcttcgccc agcatcccgg gctggagtgg gtttccacca tccatccagc
     5101 catcttcgat gccaacgcca accttgcgaa ggtcggccgc ctccccgggt tcaacaagca
     5161 ggccttcctc gatggcctgt tcctgcccgg cacccaggac aagggcttct ggatccagca
     5221 ggaatcaacc ttcttccgcc gcagcctgtg gcagcgcgcg ggcgggcgca ttcccgacta
     5281 cccgctggcc ggcgatttcg ccctgtggtg cgaattctac aagcatgccg atctggtcgc
     5341 ggtggaatac cccctcagcg gcttccgcat gctggaaggc cagaggtcgg gcgatctcga
     5401 gcaatacctg cgcgaagggg cccaggccct tcagcggctg cgcgaacacg aaggccatcc
     5461 cgcccgccgg gtggcatgca agaccgattt ctaccccggg cgcatcgtga tgaacacggc
     5521 ccgcaaccgg atcggcgcgc cctggcacat cgtcgatcac gagttcgccg cggaaaccga
     5581 cctgcccctg tggtgaccgg cacggcgccc gcgcctattc cacggtgacc gacttcgcca
     5641 ggttgcgcgg ctggtcaaca tcggtgccct tggcgcaggc gacgtgatag gccagcaact
     5701 ggaccggcac cgcatagacc agcggtgcga tcagcgggtg gaccttgggc atctcgatcg
     5761 tggccaggca gccctcgccc gcctcggcca gcccttcggc atcggagatc agcacgatct
     5821 tcccgccccg cgcgcgcacc tcctgcatgt tgctgaccgt cttctcgaac agcggcccgg
     5881 acggggcgag gacgatgacc ggcaccgcct cgtcgatcag ggcgatgggc ccgtgcttca
     5941 tctcgcccgc tgcataacct tcggcgtgga tgtagctgat ttccttgagc ttcagcgccc
     6001 cttccagcgc cagcggatag tcctgcccgc gcccgagata gagcacgtcg cgcgccggcg
     6061 cgatgagatg ggccatggcc gcgatctcgt cgtcatgcag cagggccgca ttaagcgcgg
     6121 atggcgtctc gatcagctgg gcgacgacat cctgttcctc ctcgcggctc atccgcccgc
     6181 gcttgaccgc gaggtgcgcg gccagtgcgg ccagcaccgc cagctggcag gtaaaggcct
     6241 tggtcgaagc cacgccgatt tccggcccgg cgtgggtcgg cagcagcagg tcggcctcgc
     6301 gggccatcga actggtcggc acgttgacga ccacggcaat ggtctgcccc gccgccttgc
     6361 agtggcgcag cgcggccagc gtgtcggccg tctcgcccga ctgcgaaatg aacagcgcga
     6421 ggccgcccgg ctcgagcacc ggatcgcggt aacggaactc gctcgccaca tcgatgtcga
     6481 ccgggaggcg cgcgaactgc tcgaaccagt acttggcgac gagccccgcg taataggagg
     6541 tcccgcaggc aacgatggtc acccggttga tctgcgagag gtcgaagtca agctgcggca
     6601 gggccaccga ctggtcgacc tgacgcacat agctgcgcag ggtctgggcg accacggtcg
     6661 gctgctcgaa gatctccttc tgcatgaagt ggcggtaatt gcccttctcg atcgccgccg
     6721 ccgacgcgcc cgaggcgacg atcgggcggg tgaccggccg gttcgccgcg tcatagacct
     6781 gagcgccctc gcgggtgatc accacccagt cgccctcgtc gaggtaggag atccgctggg
     6841 tcagcggcgc cagcgccagc gcgtcggagc ccaggtaggt ttccccgtcg ccatagccga
     6901 ccaccagcgg cgagccgagc cgcgcgccga tcagcatgtc gggatgggcc ttgaacgcga
     6961 tggcgagggc aaaggcgccg cgcagttccg gcaggaccgc ctgaaccgct gcttcgggcg
     7021 acttgccggc ctcgacctgc acggacagga gatgggcgac cacctcggta tcggtatcgc
     7081 tctcgaagtg ccggcccctt gcgatgaggg cttcacgcaa ggggcggaaa ttctcgatga
     7141 tcccgttgtg gaccagcgcg agatgctcgg tcccatgcgg gtgtgcattg gccgcggtcg
     7201 gcgcaccgtg ggtggcccag cgagtgtggg caatgcccgt ggtcccgggc gcgggatggc
     7261 gcgccagctc gaccaccagg ttctgcagct tgcccggcgc gcggcggcgg atcagctccc
     7321 cgccatggac cgtgcagacc cccgcgctgt catagccgcg gtattccatc cggcgcaggc
     7381 cgtcgaccag ccggtccgcc acttcgtcct tgccgacaat gccgatgatg ccgcacatgc
     7441 gtggtaacag cctttcagat caggtgtagg gaatgcggat gcccgggata ttggccggga
     7501 attccgcgga attgcgggtc acgaggatcg ccccgtgctc ctgcgccgag gcgaagacga
     7561 gcgcggccgg caggctcagc cccggccgct cggccttgag ccccgctgcc cggcgggcaa
     7621 tctccggggt aacctcgcgg atcgcgaagt tggcaaggaa ccgctc
//
LOCUS       DATNBV010000489         4400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1563055,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000489 DATNBV010000000
VERSION     DATNBV010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563055"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VN627_11520"
     CDS             <1..264
                     /locus_tag="VN627_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03925.1"
                     /translation="GLHIAAILYYRFIRHDNLVSPMLTGRRVFAEAVTTPRIASLWLA
                     LVCAVLAAALAVWVSWGLPPFATPFPWDRPAAAAAMPDASSYM"
     gene            393..1106
                     /gene="modA"
                     /locus_tag="VN627_11525"
     CDS             393..1106
                     /gene="modA"
                     /locus_tag="VN627_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXD03926.1"
                     /translation="MVTAQPPRAPVVLAAASLQESLGAAADAWARHGHPRPVLSFAAS
                     SALARQVEAGAPADLFVSADEEWMDYLAQRRLIRLETRADFLANRLVLIAPAASRLRL
                     AVGPRMALGAALGSGRLAVADPDSVPAGRYAKTALMRLGAWPQVAGRLARAENVRAAL
                     ALVARGETPLGVVYATDALAEPRVRVVSEFPASSLAPITYPLARLARADNPEGEAFRR
                     FLLSAEARAIFRRYGFGAR"
     gene            1107..1796
                     /gene="modB"
                     /locus_tag="VN627_11530"
     CDS             1107..1796
                     /gene="modB"
                     /locus_tag="VN627_11530"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HXD03927.1"
                     /translation="MLAADEWQIVALSLKVSAVAVGLTLPLAFALAWVLARSRMPGKV
                     LLDALVHLPLVVPPVVTGWLLLLAFGANGPIGGWLERALGVTVMFRWTGAALAAAVMA
                     LPLMVRAIRLSLEAVDPRLEAAARTLGAGPWRAFATITLPLALPGVLAGAVLGFARSL
                     GEFGATITFVSNIPGETQTLPLAIYAALQVPGGESVVTRLAAISVLLSLVALVASEVL
                     ARRAGRGAHVL"
     gene            1786..2412
                     /locus_tag="VN627_11535"
     CDS             1786..2412
                     /locus_tag="VN627_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXD03928.1"
                     /translation="MSFDIAVTLRRARRTIAARITSEAPVVALFGPSGAGKTTVLDMV
                     AGLVRPDAGRIAVGGEVLFDSAGGVNLAPERRACGYVFQDLRLFPHRSVGQNLRYGEA
                     LAPVERRFMGLDEAAGFLGIGHLLDRRPRSLSGGEAQRVAIGRALLAGPRFLVLDEPV
                     SSLDRARREEILGVIARIRDGLGLPILYVTHDERELARLGAEVVQIGD"
     gene            2445..3743
                     /gene="dctA"
                     /locus_tag="VN627_11540"
     CDS             2445..3743
                     /gene="dctA"
                     /locus_tag="VN627_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HXD03929.1"
                     /translation="MSLAEQDLPPVHRARHWYTHLYVQVLAAILAGVLLGQFAPATGE
                     ALKPLGDAFIKLVKLVIAPVIFLTISTGIAGMRDLGAVGRVAAKALAYFLVLSTFALI
                     VGLVVANVVRPGAGLNIDPASLDPAKVADYAHQAHETTIAGFLLAMIPETLLSALTQG
                     NILQVLVVAILFGIALATAGQRGEPVLRVLDHLSVVVFRLVEIVMRFAPIGAFGAMAF
                     TIGKYGLASLAHLGLLVATFYLTSLLFVVVVLGLVARLAGFSILRLISYLRGELLLVL
                     GTSSSESALPSLMEKLERAGCPKAIVGLVVPTGYSFNLDGTNIYMTLAALFIAQACNV
                     ELTLGQQLLLLGVAMLSSKGAAGITGAGFITLAATLSIVPTVPVAGMALILGVDRFMS
                     ECRSLTNFIGNAVATLVVARWEGMLDRAALASALAGGPAE"
     gene            3740..>4400
                     /locus_tag="VN627_11545"
     CDS             3740..>4400
                     /locus_tag="VN627_11545"
                     /inference="COORDINATES: protein motif:HMM:NF023426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HXD03930.1"
                     /translation="MKILFIHQNMPGQFKHLAPAMARAGHEVRFLTQRGDVDLPGVRR
                     ATYGKPRSAHASTHHYVRLFENSVLAGQQVVRACMQFQREGWVPDVIVAHPGWGEALF
                     VKDVWPSVPLLNFCEFYYGGEGSDTGFLPEEPADIDSICRVRARNAAHLLSLEACDAG
                     ITPTQWQLSRHPAPFRDKIRVIFDGIDCGVVRPNPKSSFALPGGRVLTAEDEVVTYVA
                     RN"
BASE COUNT          544 a         1494 c         1577 g          785 t
ORIGIN      
        1 ggcctgcaca tcgcggcgat cctctactac cgcttcatca ggcacgacaa cctggtgagc
       61 ccgatgctga ccgggcggcg cgtcttcgcc gaagcggtga ccacgccccg catcgcgtcc
      121 ctgtggctgg cgctggtctg cgctgtgctg gccgcggcgc tggcggtgtg ggtttcgtgg
      181 ggcctgccgc cctttgccac gcccttcccg tgggatcggc ccgccgccgc ggcggccatg
      241 cccgacgcat cgagctacat gtaaccggct gggccgcgct ttcgggcttc cgtcctcgcg
      301 gcttacgcta tatctgagcg gatatagtga aagctcccgg caaactcctc gccctgttcg
      361 gcctcatcct ctcgcttgtt ctcgggagcg ctatggtgac agcgcagccg ccgcgcgcgc
      421 cggtggtgct ggcggcggcc agcctgcagg aatcgctcgg cgccgcggcc gatgcctggg
      481 cccgtcatgg ccatccgcgc ccggttctct ccttcgccgc cagttccgcc ctcgcccggc
      541 aggtggaagc cggggccccg gcggacctgt tcgtttcggc cgacgaggag tggatggact
      601 acctggccca gcggcggctg atccgcctcg agacgcgggc agatttcctc gccaaccgcc
      661 tggtgctgat cgcccctgcg gcgagccggc tgcgcctcgc cgtgggcccg cgcatggcgc
      721 tgggtgcggc gctgggatcg ggcaggctgg ccgtggccga tcccgacagc gtgccggccg
      781 ggcgctatgc gaaaacggcg ctgatgcggc tgggcgcctg gccgcaggtg gcaggacggc
      841 tcgcccgggc cgagaatgtg cgcgcggccc tggcgctggt ggcgcgcggc gagacgccgc
      901 tcggtgtggt ctatgcaacc gatgccctgg ccgaaccgcg ggtccgcgtg gtgagcgagt
      961 ttcccgccag cagccttgcg ccgataacct atccattggc gcgattggca cgggccgaca
     1021 atcccgaagg cgaggcgttc cgccgcttcc tcctgtctgc cgaggcgagg gccatcttcc
     1081 gccgctacgg cttcggcgcc cgctgaatgc tcgccgcgga cgagtggcag atcgtcgcgc
     1141 tgtcgctgaa ggtcagcgcc gtcgccgtgg ggctgaccct gccgctcgcc ttcgctcttg
     1201 cctgggtgct ggcccgcagc cggatgccgg gcaaggtcct gctcgatgcg ctggttcatc
     1261 tgccactggt cgtgccgccg gtcgtgaccg ggtggctgct gctgctggcc ttcggcgcga
     1321 acgggccgat cggcggctgg ctggagcggg cgctgggggt gactgtcatg ttccgctgga
     1381 ccggcgcggc cctcgccgcc gcggtgatgg ccctgcccct gatggtgcgg gcgatccggc
     1441 tctcgctgga ggcggtcgac ccgcggctgg aagcggcggc gcggacgctg ggcgccgggc
     1501 cttggcgcgc ctttgccacg atcaccctgc cactggccct gcccggcgtg ctggccgggg
     1561 ccgtgctggg ctttgcccgt tcgctcggcg aattcggggc gacgatcact ttcgtgagca
     1621 acatcccggg cgaaacgcag accttgccgc tggccatata tgcggcactc caggttcccg
     1681 gcggcgagag cgtggtgacg cggctggcgg ccatttcggt gctgctctcg ctggtggcac
     1741 tcgtggcgag cgaagtgctg gcacggcgcg ctggacgagg cgcacatgtc ctttgacatc
     1801 gcggtgaccc tgcgccgcgc gaggcgaacc atcgccgcgc gcatcaccag cgaggcgccc
     1861 gtggtggccc tgttcggacc ttccggcgcg ggcaagacca cggtgctcga catggtggca
     1921 gggctggtgc ggcccgatgc cgggcggatc gcggtgggcg gcgaagtgct gttcgacagc
     1981 gccgggggcg tgaaccttgc gccggagcgc cgcgcctgcg gctacgtgtt ccaggatctg
     2041 cgcctgtttc ctcaccgcag cgtgggacag aacctgcgct atggcgaggc cctcgccccg
     2101 gtggagcggc ggttcatggg tctggacgag gcggccggct tcctcggcat cggtcatctg
     2161 ctggaccggc gcccgcgcag cctttcaggc ggcgaggccc agcgggtggc gatcgggcgg
     2221 gcgctgctgg cgggaccgcg cttcctcgtg ctggacgaac cggtctcctc gctcgaccgg
     2281 gcccggcgcg aggagatcct cggcgtgatc gcgcggatcc gcgatgggct tggcctgcct
     2341 atcctctacg tcacccatga cgagcgcgaa ctggcccggc ttggcgccga agtggtgcag
     2401 atcggggact agcggcggcg cggcggcgcg ctacaacggc gcgcatgagc ctcgccgagc
     2461 aagacctgcc gcccgttcac cgcgcccgcc actggtatac ccatctctac gtgcaggtgc
     2521 tggccgcaat cctggccggg gtgctcctgg ggcagttcgc gccggcaacc ggcgaagcgc
     2581 tgaagccgct gggggatgca ttcatcaagc tggtcaagct ggtgatcgcc ccggtgatct
     2641 tcctgaccat cagcaccggc attgccggca tgcgcgacct gggcgcggtg ggccgggtcg
     2701 cggccaaggc gctggcctat ttcctcgtgc tctccacctt tgcgctgatc gtcgggctgg
     2761 tcgtggccaa tgtggtccgt ccgggcgcgg ggctgaacat cgaccctgcc agcctcgatc
     2821 ccgccaaggt ggccgactat gcgcaccagg cgcatgagac gaccattgcc gggttcctgc
     2881 ttgcgatgat cccggaaacg ctgctctccg cgctgaccca gggcaacatc ctgcaggtgc
     2941 tggtcgtggc gatcctgttc gggattgccc tggccactgc cggccagcgg ggcgagccgg
     3001 tgctgcgggt gctggatcac ctgtccgtcg tcgtgttccg gctggtcgag atcgtgatgc
     3061 ggtttgcgcc cattggcgcc tttggggcca tggccttcac gatcggcaag tatggcctcg
     3121 ccagcctcgc ccacctcggc ctgctggtgg cgaccttcta cctgacctcg ctgctgttcg
     3181 tggtggtggt gctggggctg gtggctcgcc tggcggggtt ctcgatcctg aggctgattt
     3241 cctatctgcg gggcgagctg ctcctggtgc tgggcacatc gtcttccgaa agcgcgctgc
     3301 ccagcctgat ggaaaagctg gagcgggcgg gctgccccaa ggccatcgtc ggcctggtcg
     3361 tgccgaccgg ctattccttc aatctcgacg gcaccaatat ctacatgacg ctggccgccc
     3421 tgttcatcgc ccaggcctgc aacgtggagc tgacgctcgg ccagcaattg ctgctgctgg
     3481 gggtggccat gctgtcatcc aagggtgcgg cggggatcac gggcgcaggc ttcatcactc
     3541 tcgccgccac cctctcgatc gtgcccaccg ttccggtggc gggcatggcg ctgatcctgg
     3601 gggtggaccg gttcatgtcg gaatgccgca gcctgaccaa tttcatcggc aatgccgtgg
     3661 cgacactggt cgtggcgcgg tgggagggca tgcttgaccg tgcggcactg gcatcggcgc
     3721 tggcaggggg gccggccgaa tgaagatcct gttcatccac cagaacatgc cgggccagtt
     3781 caagcacctg gccccggcca tggcgcgggc cgggcacgag gtgcgcttcc tcacccagcg
     3841 cggcgatgtc gacctgccgg gcgtgcgccg ggcgacctac ggcaagccgc gctcggccca
     3901 tgcctcgacc caccactacg tgcgcctgtt cgagaattcg gtgctggccg ggcagcaggt
     3961 ggtccgcgcc tgcatgcagt tccagcgcga aggctgggtg ccggacgtga tcgtcgccca
     4021 tcccggctgg ggcgaggcgc tgttcgtcaa ggacgtctgg cccagcgtgc cgctgctgaa
     4081 tttctgcgag ttctactatg gcggcgaagg ttcggacacc ggcttcctgc ccgaggagcc
     4141 ggccgacatc gatagcatat gccgggtacg ggcgcgcaat gccgcgcatc tgctcagcct
     4201 cgaggcttgc gatgcgggga tcacaccgac gcagtggcag ctgagccggc atcccgcgcc
     4261 gtttcgcgac aagatccggg tgatcttcga cgggatcgac tgcggcgtgg tgcggcccaa
     4321 cccgaaatcg agctttgccc tgccgggagg ccgggtgctg accgcggagg acgaggtggt
     4381 gacctatgtc gcccgcaatc
//
LOCUS       DATNBV010000490         4808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4747218,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000490 DATNBV010000000
VERSION     DATNBV010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4747218"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VN627_11550"
     CDS             <1..759
                     /locus_tag="VN627_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03931.1"
                     /translation="DAARLEASLGPVSFDGTYAIAQRTVFGIDAGPRQSYGGDFVFLG
                     ASAKTGPFNVKGFAYLLDYDEAIFFANSSQTYGARATGTLPLSKLVKLNLAASYARQS
                     RYGTNPVAYSADYLAGEAALAYKTYTVTAGYEKLGADGVAGKSFQTPMATLHKFNGWA
                     DMFLVTPATGLQDIYGGVAVKFPKVKALPGLNAQVVYHDFKSDIGSVKLGDEWDASVG
                     FKLRRVGVLAKFASYDAAVGPTVNKFWLQFELAY"
     gene            1114..3609
                     /gene="nirB"
                     /locus_tag="VN627_11555"
     CDS             1114..3609
                     /gene="nirB"
                     /locus_tag="VN627_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HXD03932.1"
                     /translation="MNAPASFPETGSGQQTRERLVVIGNGMAGCRAVEEILARDPARF
                     AVTIFGAEPRVNYNRIMLSPVLAGEKAFDDIVINGADWYADNGIDLVSGDPVIAIDRE
                     RRVVVSRSGRAQPYDRLLIATGSDPFIIPVPGHDLPGVVTFRDLDDVEKMLSAADAGG
                     SAVVIGGGLLGLEAAHGLALRGMAVTVVHLMPTLMERQLDEAAGYLLKSELERRGQTI
                     VTGGDTAEIVAKDGHVSGVRLKDGREFPADIVVMAVGIRPATGLARAAGLDVERGILV
                     DDHMLTNDPAIMAVGECVQHRGQCYGLVAPLWDMCRALADAATGQPSGYTGSVTSTKL
                     KVSGIDLFSAGDFSGGDGAEDIVMRDAARGVYKRVIVKDDRLVGTVLYGDTADGSWYF
                     DLLKKGEDISPIREALIFGQAFAAGGALADPNAAVAALSDDAEICGCNGVSKGTVVAT
                     IQQGACSLDAVRSQCKASASCGSCTGLVESLLNLTLGGEVQSGPKTLCKCTTFTHADV
                     RRLILEKNLKVIPQVMQELGWSTPDGCASCRPALNYYLLCALPGDYEDDQRSRFVNER
                     MHANIQKDGTYSVVPRMWGGLTNPRELRAIADVVEKYNAPMVKVTGGQRLDIFGIKKE
                     DLPAVWADLNAAGMVSGHAYGKSLRTVKTCVGSEWCRFGTQDSTGLGVKLERMTWGSW
                     MPHKFKIAVSGCPRNCAEATIKDFGVVCVDSGYELHVGGNGGIKVRATDLLCKVETEA
                     EAMDVCAAFIQLYREQAWYLERTAPWIERVGLDHVKAALFDDPGAVAALAARFRFSQT
                     FMQDDPWAKRAAGERADLHQHLAEVRPLQVEIV"
     gene            3609..3950
                     /gene="nirD"
                     /locus_tag="VN627_11560"
     CDS             3609..3950
                     /gene="nirD"
                     /locus_tag="VN627_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HXD03933.1"
                     /translation="MIGEWLDIGPVGQIAPGTARTLAVRDGAEIAVFHTLDNRFYALV
                     NACPHKQGPLSQGIVHGNVVTCPLHNWNISLKTGEALGSDEGCVPTIPLKVDAGRLYL
                     LRESVIGRKAA"
     gene            3950..>4808
                     /locus_tag="VN627_11565"
     CDS             3950..>4808
                     /locus_tag="VN627_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXD03934.1"
                     /translation="MREVRTTCAYCGVGCGIRARVTGERAVLIKGDPDHPANYGRLCS
                     KGTHLAETVGLEGRLLAPMIGERESTWDEALDLVSARMKATIAEHGPDAVAFYVSGQV
                     LTEDYYVANKLMKGFVGSGNIDTNSRLCMASAVAAHNRAFGEDVVPCTYDDLDEADLI
                     LLVGSNTAWCHPVIWQRIEAAREKRGTRLVVIDPRRTETAEQADLHVQLAPDGDVALF
                     NDLLAEMAARGLVDEGYIAEHCEVPDDFAPFRRSRRRERQRDLGAKPLSRSGVGTHGS
                     VASAAIHPQP"
BASE COUNT          827 a         1689 c         1508 g          784 t
ORIGIN      
        1 gacgccgccc gcctcgaggc cagcctcggt ccggtcagct tcgacggcac ctatgcgatc
       61 gcgcagcgca ccgtgttcgg catcgatgcc gggccgcgcc agtcctacgg cggcgatttc
      121 gttttcctcg gcgcgagcgc caagactggc ccgttcaacg tcaagggttt cgcctacctg
      181 ctcgactacg acgaggcgat cttcttcgcc aacagctcgc agacctatgg cgcccgtgcc
      241 acgggaaccc tgccgctcag caagctggtc aagctgaacc tggccgccag ctatgcgcgc
      301 cagtcgcgct atgggaccaa cccggtcgcc tactcggccg actacctggc cggtgaggcc
      361 gccctggcct acaagaccta caccgtcacc gccggctacg aaaagctcgg ggccgacggc
      421 gttgcgggca agtcgttcca gacgccgatg gcgaccctgc acaagttcaa cggctgggcc
      481 gacatgttcc tcgtcactcc ggcgaccggg ctgcaggaca tctatggcgg cgtcgcggtg
      541 aagttcccca aggtcaaggc attgccgggg ctcaacgccc aggtcgtcta tcacgacttc
      601 aagagcgaca tcggctcggt gaagctgggc gacgaatggg atgccagtgt cggcttcaag
      661 ctccgccggg tcggagtgct ggccaagttc gccagctatg atgccgcggt cggcccgacg
      721 gtcaacaagt tctggctcca gttcgagctc gcctactgat ccttcccgac cggggagggg
      781 gaccagccgc aggctggtgg aggccacgcg cggttatccg gtctgcccgc gggataactc
      841 gggaagaccg ctgcgagccc tccgccatgc cttgcatggt tcccctcccc ccgtttcgaa
      901 gaggcattgc tgcgctgcaa aatatcttct tgccaattgc cacccaaatg ccatagattc
      961 gccgttgaag gccaaggtgc cgcccaaggt cgggcggacc agcacccggc ccgaacatca
     1021 tcacacgcgt ggcaacgaag ccgcccgtac cgctccctca caggaccggt ccgaggcggc
     1081 tttttcgtgt tgcgcagatg caggagacgc cagttgaacg cccccgccag cttccccgaa
     1141 accggctccg gccagcagac gcgcgaaagg ctcgtcgtga tcggcaacgg catggccggc
     1201 tgccgcgcgg tcgaggaaat ccttgcccgc gatcccgccc gattcgccgt caccatcttc
     1261 ggcgcagagc ctcgggtgaa ctacaaccgg atcatgctct cgccggtcct ggctggcgag
     1321 aaggcgttcg acgacatcgt gatcaacggg gcggactggt atgccgacaa cggcatcgac
     1381 ctcgtctcgg gcgacccggt gatcgccatc gaccgtgagc gccgggtggt cgtcagccgc
     1441 tcgggccgcg cccagcccta tgaccggctg ctcatcgcca ccggctccga ccctttcatc
     1501 atccccgtcc ccggtcatga cctgcccggg gtcgtcacct tccgcgacct tgatgacgtc
     1561 gagaagatgc tcagcgccgc cgatgcgggc gggagtgcgg tggtcatcgg gggcggcctg
     1621 ctcggcctcg aagccgccca cggcctggcc ctgcgcggca tggcggtcac cgtcgtccac
     1681 ctcatgccca ccttgatgga acgccagctc gacgaggccg ccggctacct cctcaagagc
     1741 gaactcgaac gccgcggcca gaccatcgtc accggcggcg acactgccga gatcgtggcc
     1801 aaggacggcc atgtttccgg cgtccgcctc aaggacggcc gcgaatttcc cgccgacatc
     1861 gtggtcatgg ccgtcggcat ccgcccggcc accggcctcg cccgcgctgc cgggctggac
     1921 gtcgaacgcg gcatcctggt cgacgatcac atgctcacca acgacccggc gatcatggcc
     1981 gtcggcgaat gcgtccagca tcgcggccag tgctacggcc tggtcgcccc gctgtgggac
     2041 atgtgccgcg ccctggccga tgccgcgacc ggccagccct cgggctacac cggctcggtc
     2101 acctcgacca agctcaaggt ctcaggcatc gacctgttct ccgccggcga tttctccggc
     2161 ggcgacggcg cggaagacat cgtcatgcgc gatgccgccc ggggcgtgta caagcgcgtc
     2221 atcgtcaagg acgaccggct ggtcggtacc gtgctctacg gcgataccgc cgacggttcg
     2281 tggtacttcg acctcctcaa gaagggcgag gacatctcgc cgatccgcga ggccctgatc
     2341 ttcggccagg ccttcgccgc gggaggcgcc ctcgcggacc ctaatgccgc cgttgccgcc
     2401 ctgtccgatg atgcggagat ctgcggttgc aacggcgttt ccaagggcac tgtcgtcgcc
     2461 accatccagc agggcgcttg cagcctcgat gcagtgcgct cgcagtgcaa ggccagcgcg
     2521 agctgcggat catgcaccgg gctcgtggaa agcctgctga atctgacact tggtggcgag
     2581 gtccagtccg gccccaagac cctgtgcaag tgcaccacct tcacgcacgc cgacgtgcgc
     2641 cgccttatcc tggagaagaa cctcaaggta attcctcagg taatgcagga acttggctgg
     2701 tccaccccgg atggctgcgc aagctgccgc ccggccctca actattacct cctctgcgcc
     2761 ttgcccggcg actacgagga cgaccagcgc agccgcttcg tcaacgagcg gatgcacgca
     2821 aacatccaga aagatgggac ttattcggtt gtcccacgca tgtggggcgg tctcaccaat
     2881 ccccgcgaac tgcgcgccat cgccgacgtg gtggaaaaat acaacgcccc gatggtcaag
     2941 gtcaccggcg ggcaacgcct tgatatcttt ggcatcaaga aggaagacct gcccgccgtc
     3001 tgggccgatc tcaacgccgc cggcatggtc tcgggccatg cctacggcaa gtccttgaga
     3061 acagtgaaaa cctgcgtcgg ctcggaatgg tgccgcttcg gcacgcagga ttccaccggc
     3121 ctcggcgtca agctggagcg gatgacctgg ggctcgtgga tgccccacaa gttcaagatc
     3181 gccgtctccg gctgccctcg caattgcgcg gaagccacga tcaaggactt cggcgtggtc
     3241 tgcgtcgaca gcggctacga gctgcacgtc ggcggcaatg gcggcatcaa ggtccgggcg
     3301 accgacctcc tctgcaaggt cgagaccgag gccgaagcga tggacgtctg cgccgccttc
     3361 atccagctct accgcgaaca ggcctggtac ctcgaacgca ccgcgccgtg gatcgaacgg
     3421 gtcgggctcg atcacgtgaa ggcagccctg ttcgacgatc ctggtgccgt cgcggcgctc
     3481 gcggcccggt tccgcttctc gcaaaccttc atgcaggacg atccctgggc caagcgcgcc
     3541 gcgggcgaac gggccgacct gcaccagcac ctggccgagg tgcgccctct tcaagtggag
     3601 atcgtgtgat gatcggtgag tggctcgata tcggccccgt gggccagatc gcccccggca
     3661 cggcgcgcac cctggccgtc cgggacggcg cggagatcgc ggtgttccat acgctggaca
     3721 atcgcttcta tgccctggtc aatgcctgcc cgcacaagca gggcccgctc agccagggca
     3781 tcgtccacgg caacgtggtc acctgcccgc tgcacaactg gaacatctcc ctgaagaccg
     3841 gcgaggcgct cggcagcgat gaaggctgcg tgccgacgat cccgctcaag gtggatgccg
     3901 gccgcctcta tctgctgcgc gaatccgtca tcgggcggaa ggcggcctga tgcgcgaggt
     3961 ccgcaccacc tgcgcttatt gcggtgtcgg ctgcggcatc cgcgcgcggg tgacgggtga
     4021 acgcgctgtc ctcatcaagg gggaccccga tcacccggcg aactatgggc gactctgctc
     4081 caagggcacg caccttgctg aaacggtcgg ccttgaaggc cgcctgctcg ccccgatgat
     4141 cggcgaacga gaatccacct gggacgaggc gctcgatctc gtttccgcca ggatgaaggc
     4201 caccatcgcc gaacacgggc ctgatgcggt ggccttctat gtctccggcc aggtcctcac
     4261 cgaggactac tacgttgcca acaagctgat gaaaggcttc gtcggcagcg gtaacatcga
     4321 taccaattcg cgcctgtgca tggccagcgc ggttgccgcc cacaaccgcg ccttcggcga
     4381 ggacgtggtc ccctgcacct acgacgatct ggacgaggcc gacctcatcc tgctggtcgg
     4441 ctcaaacacg gcctggtgcc acccggtcat ctggcagcgc atcgaggccg cccgcgagaa
     4501 gcgcggcacc aggctcgtgg tgatcgatcc gcgccgcacc gaaaccgccg aacaggccga
     4561 cctccacgtg cagctcgcgc ccgatggcga cgtggcgctg ttcaacgacc tgctggcgga
     4621 gatggccgcg cgaggactgg tcgacgaggg ttacatcgca gaacactgcg aggtcccaga
     4681 cgatttcgca ccgttccgcc gctcccgcag gcgggagagg cagcgagact tgggggcgaa
     4741 gccccttagt cgcagcgggg tgggcacgca cggcagcgtc gcctcagccg caatccaccc
     4801 ccaacccc
//
LOCUS       DATNBV010000491         8600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3774935,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000491 DATNBV010000000
VERSION     DATNBV010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3774935"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..899
                     /locus_tag="VN627_11570"
     CDS             105..899
                     /locus_tag="VN627_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXD03935.1"
                     /translation="MSNPPGSFIWYELMTSDAAGAAAFYGAVVGWEFHPPQAGAPIEY
                     WHIGRSDGGSAGGMLQLTADMLTGGAHSGWLPYLNVPDVDEAVAAIRDDGGATIMPKT
                     TIDVGAFALVTDPQGAPIYVMTPVPPADNPHATSDVFSPSEPQHVRWNELSTSDVDAA
                     KAFYSAHFGFEFNNSMPMGEMGDYWFIDHHGQVLGAVMRQRDPAGRPQWLPYFGVPSA
                     LAAETAIRANGGTVIAGPHEVPGGDWVVMGIDPQGAAFGVVGPREA"
     gene            902..1534
                     /locus_tag="VN627_11575"
     CDS             902..1534
                     /locus_tag="VN627_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXD03936.1"
                     /translation="MAEYTFFTNPMSRGQIARWALHEAGADYAQVLVELGHKPPALVA
                     ANPMGKVPTLVHHALDGERIVTEAAAICAYLAEEHPGAELTPQDAERADYFRWLFFAA
                     GPLEQAVTARSLGFAPTDPRQQGMAGFGTYERTIDSFESLFADGRAWVCGERFTMADV
                     YVGSHVDWGLNFGSLPPRVAFVAYAERVQQRPAYKAAKAVDNALIAEMKK"
     gene            1531..2055
                     /locus_tag="VN627_11580"
     CDS             1531..2055
                     /locus_tag="VN627_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXD03937.1"
                     /translation="MSDPHKVRVCLWYEGAAREAAEFYAATFPDSRVETVQQAPSDYP
                     DGHQGDELVVLFTVLGIPCFGLNGGPQFKHSEAFSFQVYTDNQEETDRYWNAIVGNGG
                     QESACGWCKDKWGISWQITPRVLTDALGDPDREASKRVFEAMMTMRKIDIAAIEAARN
                     GRGRAAAGEAEEHK"
     gene            2052..2714
                     /locus_tag="VN627_11585"
     CDS             2052..2714
                     /locus_tag="VN627_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXD03938.1"
                     /translation="MTPVLALYGHPFSSYTWKAQIAFHAAGVNYEFREVGPDQPANGE
                     FLVSHAGPWGKFPVLVDGEQVLFEATVIIEHLAIHHAPQAGLLPRDPVAALPLRMLDR
                     VFDNYVMSPMQAVVDEYLRNAEAPDLDRCGEARGRLERSYGWLETWLAGWNRGGPTTL
                     VECAAAPALFYAQWVHPIGAEFPLLRAWRAQLNRRPEVARCIEAARPFRGYFPLGTPQ
                     ED"
     gene            2718..3104
                     /locus_tag="VN627_11590"
     CDS             2718..3104
                     /locus_tag="VN627_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXD03939.1"
                     /translation="MAELNHTIVWVRDQKTAARFFAGVLGLPEPTTFSHFDVVQMVNG
                     VSLDFADKDGEISAQHYAFLVSDAEFDAGFAWVKERGLPYWADPARSHPGEINHHWGG
                     RGVYFEGPDGHFLELITKPYGKIEDL"
     gene            3110..3568
                     /locus_tag="VN627_11595"
     CDS             3110..3568
                     /locus_tag="VN627_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXD03940.1"
                     /translation="MHELMISRHIAATPDAVWDVMVNRTGEWWCPVPWRAEVTHWDRR
                     PGGRCDMVMYGPDGEVMPQEGVFLAWDEGRRFASTDAVKGDLEPAGPFMIGIWEVEPD
                     GEGSRYTARARHWSEDTMRQHEDMGFQQGWSACADQLAAICEREAGSQGD"
     gene            complement(3581..4171)
                     /locus_tag="VN627_11600"
     CDS             complement(3581..4171)
                     /locus_tag="VN627_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HXD03941.1"
                     /translation="MRHDRFHGRVSGGPSGQRRGCERPGCRQEGEFRAPGHRPPGFDG
                     PGDYRWFCLDHVREFNAGYDWFAGMSADEIIAAQHPAAGWDGESRAFRPTAGVDQAPR
                     WADFKDPLDAINLRARTFRDAVRPDAPRLAPEERRAFETLGLGHDADRKALRARYSQL
                     VRRYHPDRNGGDRSMEAKLQAVVEAYNLLKASALFA"
     gene            4211..4483
                     /locus_tag="VN627_11605"
     CDS             4211..4483
                     /locus_tag="VN627_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HXD03942.1"
                     /translation="MSGPLAREIERLLTQQFAPTHLAVINDSANHRGHTGDDGTGESH
                     FTVEIESGMFAGVGRLQRQRMVNSALGDIPGGRVHALAIKARAPGE"
     gene            4517..5425
                     /locus_tag="VN627_11610"
     CDS             4517..5425
                     /locus_tag="VN627_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03943.1"
                     /translation="MLHHATEAALSPAERRAHYFVPDEAFKRSITAVPAAIFRAERAR
                     AFAPDCPTGFIECDQSEALGYAWPATTPAMLARYVVVRAGERLDHRLQASGLIYYVLR
                     GAGTVTCAGEEIAWGMGDGFCLPGNADVTLASSEGAILMLFSDEPLLHLLRATGAEGA
                     TGTIRPTLFPVHVIDRLIGDIHDQTGEQITAGKSVTYVTDELAGQQTTTPTLIGGINS
                     LEPGGDQRPHKHSSAALTLPVWSEGLHSTLNGEVIPWEPDTLFVTPANAVHSHHNRGP
                     GMMRSFVVQDTALHSGLRSITFAWGD"
     gene            complement(5469..6611)
                     /locus_tag="VN627_11615"
     CDS             complement(5469..6611)
                     /locus_tag="VN627_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="12-oxophytodienoate reductase"
                     /protein_id="HXD03944.1"
                     /translation="MTADITEPLFQPLTVRDVTIPNRTVMAPMGRNFCTDHVLDAASP
                     AYYRRRIEGGVGMIITEATGVDHPVSVDRGNTPRMDGDAALAMWRKVVDEVHSAGGII
                     ANQLFHQGMLYGGADPEVLGNSMRPSGTIGTPGPTSYTPEYIEQARRSTRPMTDSEIA
                     DVIAAFGRSARNAVSVGFDAIDVHGAHGYMVDGFLWGHSNQRTDRWGGSQGQRTAFAV
                     EVIKTVRAEMRDGMPLFFRFSQHKSSNYDARTAETPQDLEAMLGPLADAGVDVFDASI
                     RRFWQPAFEGSDLNLAGWARKLTGKPVMAVGSVGLGSTTTETFMKVGTELVADNVPLL
                     MERFNSGEFDLIAIGRSLIGDPDYVNKLRSGEKPTAFSRDHLATLY"
     gene            complement(6673..7491)
                     /locus_tag="VN627_11620"
     CDS             complement(6673..7491)
                     /locus_tag="VN627_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXD03945.1"
                     /translation="MTVSAAEERTVVRVEREGPLAILTMDDQPTRNGLSAALTRQLVA
                     ACSAINADMSIKCAILTAAGDVFCAGGNLKDMYAKANHFAGNAAEIRRVYLTGVQTIA
                     KALYDLEVPIIAAVNGAAMGAGMDFATMCTLRIASEKAKFAESFIKLGLTSAAGGAWF
                     LNRAVGPQRAAELTLSGETFDAHRAVEIGLVLKAVPHESLLDEARALAAKITCHPAHS
                     IRLNTRLLRESARLDLGSALEIAAGMQAVVQQTEDQYEAVCAAVEKRKPNFVGR"
     gene            complement(7488..8219)
                     /locus_tag="VN627_11625"
     CDS             complement(7488..8219)
                     /locus_tag="VN627_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD03946.1"
                     /translation="MRRNTMEQRRVLVIGASGDVGRGIVGAVLAKGWAAVAAGRSREH
                     LASIFPPRDGLTLVEGSLSTEASTAALWQAATAAGGPIDAIAVSVNAPMRVARLDDWS
                     AEDMAELFAANLLTHFNAVKILHPLLGADGVFIGIGGGTADIILPDLAPISVPMAGLR
                     MLYRGFARERKGGPHLRELMVLSMVNGASKRDQAQPEWLTDEEVGRHVCAIIASPDQF
                     PGPILKVRSREQVGQPEPAEGNTRS"
     gene            complement(8313..>8600)
                     /locus_tag="VN627_11630"
     CDS             complement(8313..>8600)
                     /locus_tag="VN627_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXD03947.1"
                     /translation="WHWHWDGGYRDPRHKIGLGAGTEWLQSIATNVKVPVLLAWCELS
                     EVVDAEGIAALKAEIPQLEVEVIPGAHHMIVGDENDVFADALIGFLERHAL"
BASE COUNT         1466 a         2878 c         2842 g         1414 t
ORIGIN      
        1 gactttgctg cccagctcga gggagaaatg ctggaaatgc ccttcgacgg cgcgcgcatg
       61 atcttcggcg ggttcgagcc ggttatagac attggagaac agcagtgagc aatccgccag
      121 gcagcttcat ctggtacgaa ctgatgactt ccgacgccgc cggggccgcg gcgttctatg
      181 gcgctgtggt gggatgggag tttcatcccc ctcaggccgg ggcaccgatc gaatactggc
      241 acatcggccg cagcgacggc ggcagcgcgg gcggcatgtt gcagctgacc gcggacatgc
      301 tgaccggagg cgcccactcc ggctggctcc cctatctcaa cgtgcctgat gtggacgagg
      361 ccgtcgccgc gatccgcgat gatggcgggg caacgatcat gcccaagacg accatcgatg
      421 tcggcgcctt cgcgctggta accgatcccc agggcgcacc gatctacgtg atgactcccg
      481 tgccgccagc cgacaatccc catgccacca gcgacgtctt ctctcccagc gagccccagc
      541 acgtgcgctg gaacgagctt tcgaccagcg acgtggacgc cgccaaggcc ttctattccg
      601 cgcatttcgg cttcgaattc aacaattcga tgccgatggg cgaaatgggt gactattggt
      661 tcatcgatca ccacggccag gttctcggcg ccgtcatgcg ccagcgcgat ccggcggggc
      721 ggccgcagtg gctgccctat ttcggagtcc cgtccgccct tgccgccgag accgcaatcc
      781 gtgcgaacgg cggcacggta atcgccgggc cgcatgaagt cccgggcggt gactgggtgg
      841 tgatggggat cgatccgcaa ggcgcggcct tcggggtggt tgggccacgg gaggcatgag
      901 aatggccgaa tacaccttct tcaccaatcc gatgagccga gggcagattg cccgctgggc
      961 gctgcacgag gccggggccg actatgcgca ggtgctggtc gaactggggc acaagccccc
     1021 tgccctggtg gccgccaacc cgatgggcaa agtaccgacc ctcgtgcacc acgcgcttga
     1081 tggcgagcgg atcgtaaccg aggccgcggc gatctgcgcc tacctggccg aggaacaccc
     1141 gggcgccgaa ctcacccccc aggatgccga gcgggccgac tactttcgct ggctgttctt
     1201 tgccgccggc ccgctggagc aggcggtcac ggcgcgctcg ctcggcttcg caccgaccga
     1261 tccgcgccag cagggcatgg ccggcttcgg cacctacgag cgcacgatcg actcattcga
     1321 gagcctgttc gccgacggcc gggcctgggt ctgcggggag cggttcacca tggccgacgt
     1381 ctatgtcggc agccatgtcg actggggcct gaacttcggc tcgcttccac cgcgcgtggc
     1441 cttcgtcgcc tatgccgagc gggtccagca gcgccccgcc tacaaggcgg caaaggcagt
     1501 cgacaacgcg ctgatcgcgg agatgaagaa atgagcgatc cccacaaggt ccgcgtctgc
     1561 ctgtggtacg aaggcgcggc gcgcgaggcg gctgagttct acgcggcgac ctttcccgac
     1621 agccgcgtcg aaaccgtgca gcaggccccg tcggactacc ccgacggcca ccagggcgac
     1681 gaactggtgg tgctgttcac cgtgctcggc atcccctgct tcgggcttaa cggcgggccg
     1741 cagttcaagc attccgaggc cttttccttc caggtctata ccgacaacca ggaggagact
     1801 gaccgctact ggaacgccat cgtcggcaac ggcggacagg aaagcgcctg cggctggtgc
     1861 aaggacaagt ggggcatctc ctggcagatc accccgcgcg tgctgaccga tgcactgggc
     1921 gatcccgacc gggaggcctc gaaacgggta ttcgaggcaa tgatgaccat gcgcaagatc
     1981 gatatcgcgg cgatcgaagc agcgcggaat ggcagaggtc gcgcagccgc aggcgaagcg
     2041 gaggaacaca agtgaccccc gtgctcgcgc tctacggcca tccattctca agctacacgt
     2101 ggaaggccca gatcgcgttc catgcggcgg gtgtgaatta cgagttccgc gaggtcggac
     2161 ccgatcagcc cgccaacggc gaatttctgg tcagccatgc cgggccgtgg ggcaagtttc
     2221 cggtgctggt cgatggcgag caggtgctgt tcgaagccac tgtcatcatc gaacacctgg
     2281 ccatccacca cgcgccacag gccggactgc tgccgcgcga tcccgttgcc gccctgccgc
     2341 tgcgcatgct cgaccgggtg ttcgacaatt acgtgatgtc gccgatgcag gccgtggtcg
     2401 acgaatatct gcgcaatgcc gaagcgccgg acctcgaccg ctgcggggag gcgcgcgggc
     2461 ggctggagcg gtcctatggc tggctggaga cctggttggc cgggtggaac cgcggtggcc
     2521 cgaccacgct ggtcgaatgc gccgcggctc cggcgctgtt ctatgcccag tgggtccacc
     2581 ccatcggcgc ggagtttccg ctgctgcggg cctggcgcgc gcagcttaac cgccggccgg
     2641 aagtggcgcg ctgcatcgag gcggcccgcc cgtttcgcgg ctattttccc ttgggcacac
     2701 cacaagagga ctgagcaatg gccgagctca accacaccat cgtctgggtc cgcgaccaga
     2761 agaccgccgc ccggtttttc gccggagtgc tgggcctgcc tgagccgacc acgttctcgc
     2821 acttcgacgt ggtccagatg gtcaacggcg tctcgctcga ctttgccgac aaggacggcg
     2881 agatcagcgc gcagcactat gctttcctcg tttccgatgc cgagttcgac gccggcttcg
     2941 cctgggtaaa ggaacgcggg ctgccctatt gggccgatcc ggcgcgcagc caccccggcg
     3001 agatcaacca ccactggggc gggcgcggcg tctatttcga ggggccggac ggccatttcc
     3061 tcgaactgat caccaagccc tatggcaaga tcgaggacct gtaagcgcca tgcacgaact
     3121 gatgatcagc cgccacatcg ccgccacgcc agacgcggtc tgggacgtca tggtcaaccg
     3181 caccggcgag tggtggtgcc ccgtgccctg gcgcgccgag gtcacgcatt gggaccggcg
     3241 accgggcggt cgctgcgaca tggtgatgta tggaccggat ggcgaagtca tgccgcagga
     3301 aggtgttttc ctcgcctggg acgaaggccg ccgctttgcc tcgaccgacg cggtaaaggg
     3361 cgatctcgag cccgccggcc cgttcatgat cggcatctgg gaagtcgagc ccgacggcga
     3421 aggatcgcgc tacaccgccc gtgcccgcca ctggagcgag gataccatgc gccagcacga
     3481 ggatatgggc ttccagcagg gctggtccgc ctgcgccgac caactggccg ccatttgcga
     3541 gcgcgaggcg ggcagccagg gggattaggg tcagcaggac ctaggcgaac agggccgaag
     3601 ccttcagcag gttataggct tccaccactg cctgcagctt cgcctccatc gaccggtcgc
     3661 cgccgttgcg atcgggatga tagcggcgga ccagctgcga atagcgggcg cgcagggcct
     3721 tgcggtcggc atcatggcca aggcccagcg tctcgaaggc gcggcgctcc tcgggcgcga
     3781 ggcgcggcgc atcggggcgc acggcatccc ggaaggtgcg cgcccgcagg ttgatcgcat
     3841 cgagcggatc cttgaaatcc gcccagcgcg gcgcctggtc cacgcccgcg gtgggccgga
     3901 acgcccggct ctccccgtcc cagcccgcgg ccgggtgctg ggcggcgatg atctcgtcgg
     3961 cgctcatccc ggcaaaccag tcatagccgg cgttgaattc gcgcacatgg tcgaggcaga
     4021 accagcgata gtcgcccggg ccgtcaaacc cgggcgggcg gtggccgggc gcccggaatt
     4081 cgccctcctg gcggcacccc ggccgctcgc agccgcgccg ctggccgctc ggccccccgc
     4141 tgacgcggcc atggaacctg tcgtgtctca cagcgtggca aatggggaag aatcggctag
     4201 attgaaaccc atgtcaggac cacttgcccg agaaatcgaa cgactgctca cgcagcagtt
     4261 tgcccccacc cacctcgccg tcatcaacga cagcgccaac catcgcggtc acaccggcga
     4321 cgatggcacc ggcgaaagtc atttcacggt ggaaatcgag agtgggatgt ttgccggcgt
     4381 cggtcgactc cagcgccagc gaatggtcaa cagtgcgctg ggcgacattc ccggcgggcg
     4441 agtccacgcc ttggcgatca aggcccgcgc gccgggagaa tagatttccg gttcaagagc
     4501 ttgaaggaga agatcgatgt tgcaccacgc aacggaagcg gctctcagcc cggccgagcg
     4561 gcgggcgcat tacttcgttc ccgatgaggc cttcaagcgc tccatcaccg cggtcccggc
     4621 cgcgatcttc cgcgccgaac gcgcgcgggc attcgcgccc gattgcccca ccggcttcat
     4681 cgagtgcgac cagtcggagg cactcggcta tgcctggccg gcgacgaccc cggccatgct
     4741 ggcacgctat gtggtagtgc gcgccggcga acggctggat catcgcctcc aggccagcgg
     4801 gctgatctat tatgttctgc gcggcgcggg cacggtaacc tgcgcgggcg aggagatcgc
     4861 ctggggcatg ggcgacgggt tctgcctgcc gggtaacgcc gatgtcaccc tggcctcgtc
     4921 cgaaggggcg atcctcatgt tgttcagcga cgagccgctg cttcacctgc tgcgggccac
     4981 cggggccgag ggcgcgaccg gcacgatccg gcccactctg tttccggtgc atgtgatcga
     5041 ccggctgatc ggcgatatcc acgaccagac cggcgagcag atcacggccg gcaagtcggt
     5101 cacctatgtt accgacgagc tggccggcca gcagacgacc acgcccacac tgatcggcgg
     5161 aatcaactcg ctcgaacccg gcggcgacca gcgcccgcac aagcacagct cggccgcgct
     5221 gaccctgccg gtgtggagcg aagggctgca ctccacgctc aacggcgaag taatcccgtg
     5281 ggagccggac acgctgttcg tcaccccggc caacgccgtc cattcgcacc acaatcgcgg
     5341 gccggggatg atgcgctcgt tcgtggttca ggacacggcc cttcactcgg gcctgcgctc
     5401 catcaccttc gcctggggcg actgatggga cgggcggcga cccagcggcc cgcccttccc
     5461 cgccccgatc agtacagcgt ggcgagatgg tcgcgcgaga aggccgtggg cttctcgccg
     5521 ctgcgcagct tgttcacgta atctggatcg ccgataagtg aacggccgat ggcgatcagg
     5581 tcaaactcgc ctgagttgaa ccgctccatc agcagcggca cgttgtcggc caccaactcc
     5641 gtgccaacct tcatgaaggt ttcggtcgtg gtcgaaccaa ggccgaccga acccaccgcc
     5701 atgaccggct tgccggtgag cttgcgcgcc caacctgcca gattgaggtc cgatccctcg
     5761 aacgcgggct gccagaagcg gcggatcgaa gcatcgaaca cgtccacccc ggcgtcggcc
     5821 agaggcccga gcatggcctc gagatcctgc ggagtttcgg ccgttcgcgc gtcgtagttg
     5881 ctcgacttgt gctgcgagaa acggaagaag agcggcatgc cgtcgcgcat ctcggctcgg
     5941 acagtcttga tcacctcgac cgcgaaggcc gtgcgctgac cctggctgcc gccccagcgg
     6001 tcggtccgct gattgctgtg gccccagagg aacccgtcga ccatgtagcc gtgcgccccg
     6061 tggacatcga tcgcatcaaa gccgacgctg acggcgttgc gcgcggaacg gccgaaagca
     6121 gcaatgacgt cggcaatctc agaatcggtc atcggccggg tcgagcgccg ggcctgctcg
     6181 atatattcgg gcgtatagct ggtcgggccg ggggtgccga tggttcccga aggccgcatc
     6241 gaattgccga ggacctccgg gtccgcgccg ccgtagagca tgccctggtg gaacagctgg
     6301 ttggcgatga tcccgccggc cgagtgtacc tcgtcgacca ccttgcgcca catcgccaga
     6361 gccgcgtcgc cgtccatgcg cggggtattg ccgcggtcaa ccgagaccgg gtgatcgacc
     6421 ccggtggcct cggtaatgat catgcccacg ccgccctcga tccggcggcg gtaataggcc
     6481 ggcgaggcag cgtcgagcac gtgatcggtg cagaagttcc ggcccatcgg cgccatgacg
     6541 gtgcgattgg gaatggtcac atcgcggacc gtcagcggct ggaacaaggg ctcggtaatg
     6601 tctgcggtca tatgatcgca atcctttcat ctgccacctg cgcttgggcg cattggtgcc
     6661 accatctccc catcagcggc ccacgaaatt cggcttgcgc ttctccaccg ccgcgcagac
     6721 cgcttcgtac tggtcctccg tctgctggac gacggcctgc atgcccgcgg cgatctccag
     6781 cgcggagccc agatcgaggc gggcggattc gcgcagcagt cgggtgttga gccggatcga
     6841 atgggccgga tggcaagtga tcttcgcggc cagcgcacgg gcctcgtcga gcaagctctc
     6901 gtgcggcacg gccttcagga ccagcccgat ctcgactgcg cggtgagcat cgaaggtctc
     6961 gccgctcagc gtcagctccg cagcgcgctg cgggcccacc gcgcggttga ggaaccaggc
     7021 gccgccagcc gccgaagtca agccgagctt gataaagctt tcagcgaact tcgccttctc
     7081 cgaggcaatc cgcagggtgc acatcgtggc gaaatccatg cccgcgccca tggccgcgcc
     7141 gttgacagcg gcgatgatcg gcacctcgag gtcatagagc gccttggcga tggtctgcac
     7201 gccggtaaga taaacccggc ggatttccgc ggcattgccg gcgaagtggt tcgccttggc
     7261 gtacatgtcc ttgagattgc cgccggcgca gaacacatcg ccggccgcgg tcaggatcgc
     7321 gcacttgatc gacatgtcgg cattgatcgc ggagcaggcg gcgacgagct ggcgggtaag
     7381 cgccgcgctc aatccgttgc gggtcggctg gtcgtccatg gtcaggatcg caagcgggcc
     7441 ttcgcgctcg accctgacga cggtgcgctc ttcggcggcg gatacggtca tgatctggta
     7501 tttccttcag caggttcagg ttgccccacc tgttcgcggg agcgcacctt gaggatgggg
     7561 ccgggaaact ggtctggtga ggcgatgatt gcgcagacat gcctgccgac ctcctcgtcg
     7621 gtcagccatt cgggttgcgc ctgatcgcgc ttgctggcgc cgttgaccat cgacaggacc
     7681 atgagttcgc gcaggtgcgg cccgcctttc cgctcgcgcg caaaaccgcg gtaaagcatg
     7741 cgcaggcccg ccatcgggac cgagatcgga gccaagtcgg ggaggatgat atcggccgtc
     7801 ccgccgccga tcccgataaa cacgccgtcc gcgccgagca gcgggtgcag aatcttgacg
     7861 gcgttgaaat gcgtcaggag gttggctgca aacagctctg ccatgtcttc ggccgaccaa
     7921 tcatccaacc gggcaacacg catcggtgcg ttgaccgaga cggcaattgc atcgatcggt
     7981 ccgcccgctg ccgtggcggc ttgccacagt gcggctgtgc tggcttcggt ggagaggctg
     8041 ccctcgacca aggtgagccc gtcgcgtggc gggaagatgc tggcgaggtg ctcacggctg
     8101 cgccctgcgg ccacggcggc ccagcccttc gccagcacgg cgccgacaat gccgcgtccg
     8161 acatcgcccg acgctccaat cacgagaacc ctgcgctgtt ccatcgtgtt tctccgcatg
     8221 atagcccaca gcggatagcg ccccgcccgc cccagggaaa ccgccgcgcg cgtagatcaa
     8281 ggatgtgcgc gctgtgcgcg gcggcggctc agctacagcg cgtggcgttc gagaaagccg
     8341 atcagggcat cggcgaaaac gtcgttctcg tcgcccacga tcatatgatg ggcgccgggg
     8401 atgacctcca cttccagctg cggaatttcc gccttgagcg cggcaatgcc ctcggcatcg
     8461 acgacctcgc tcagctcgca ccaggccagc aggaccggga ccttgacgtt ggtagcgatg
     8521 gattggagcc actccgtccc cgcgccgagg ccgatcttgt ggcgcggatc gcgatagcct
     8581 ccgtcccagt gccagtgcca
//
LOCUS       DATNBV010000492         3456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_915156,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000492 DATNBV010000000
VERSION     DATNBV010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915156"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VN627_11635"
     CDS             complement(<1..453)
                     /locus_tag="VN627_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HXD03948.1"
                     /translation="MIIAVDGPTASGKGTIAKALARHYGLPHLDTGLLYRAVGYQCFH
                     DGGNPDNAGDALAATAFADSLLGNPALRSEETGGLASRVSVHPAVRQALYERQRSFAC
                     QPGGAVLDGRDIGTVIAPEAEAKLFVLASVKVRAQRRFLEMQARGAHVT"
     gene            complement(450..737)
                     /locus_tag="VN627_11640"
     CDS             complement(450..737)
                     /locus_tag="VN627_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HXD03949.1"
                     /translation="MILPLSHALANASGFAGMACIIFAYAYATHNKLPNPFVQHGVNL
                     AGAGLLTVSLLVNLNPASLVLEFFWASIAIWGLVKAWRARGRARGAEGPGA"
     gene            complement(734..2080)
                     /gene="aroA"
                     /locus_tag="VN627_11645"
     CDS             complement(734..2080)
                     /gene="aroA"
                     /locus_tag="VN627_11645"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HXD03950.1"
                     /translation="MSHSDPASVRPRRFAATGPLKGRIRVPGDKSISHRSIMLGALAL
                     GETRVSGLLEGEDVLSTAAAMRAMGASVMREATGDWVIHGVGVGGLLQPEAALDMGNS
                     GTSTRLLMGLVASHAITASFVGDASLSKRPMGRVIEPLSLMGADFTASPGGRLPLMMR
                     GAAPAVPITYRLPVASAQVKSAVLLAGLNTPGVTTVIEPVPTRDHSERMLRGFGARLE
                     VEVQADSKRIIRLTGEAELTGRQIVVPGDPSSAAFFVVAALLVPGSDLVIENVGLNPT
                     RAALFDVLRDMGGSIAEVDRREVGGEPVADLHVRHSPLSGIAVDRAVVPAMVDEFPIL
                     FVAAALAKGRTVTTGLEELRVKESDRIAVMRAALELAGARVTETEDGLIIDGTGGEPL
                     AGTPPGSAVATHLDHRIAMSLAVAGLASHRGVEVDDTRPIATSFPVFESLLDGLAG"
     gene            2408..2722
                     /locus_tag="VN627_11650"
     CDS             2408..2722
                     /locus_tag="VN627_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HXD03951.1"
                     /translation="MVKPEWGAKHGCPKCGTRFYDLGKDDPVTCIECGYAWHPEPVLK
                     SKQPMPYEEIQKKTETPDADLAEDDLDIDEDGDSPDNDVDLGGDDDLGVGVDSDGEPD
                     DV"
     gene            2836..2911
                     /locus_tag="VN627_11655"
     tRNA            2836..2911
                     /locus_tag="VN627_11655"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2869..2871,aa:Ala,seq:ggc)
     gene            3074..>3456
                     /locus_tag="VN627_11660"
     CDS             3074..>3456
                     /locus_tag="VN627_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HXD03952.1"
                     /translation="MFDTLSDRLCGVFDKLRGRGALKEQDVRDAMREVRIALLEADVA
                     LPVVRRFIDQVTEAAVGQSVLKSVTPGQQVVKIVNDALVEMLGGEETVGLDLNAAPPV
                     VIMMVGLQGSGKTTTTAKIAKLLKE"
BASE COUNT          585 a         1152 c         1169 g          550 t
ORIGIN      
        1 cgtgacatgg gccccgcgtg cctgcatctc gaggaagcgc cgctgcgcgc gaaccttgac
       61 gctggcgaga acgaacagct tggcctcggc ctccggcgcg atcaccgtgc cgatgtcgcg
      121 cccgtcgagc acggcgccgc ccggctggca ggcaaagctg cgctgccgtt cgtaaagggc
      181 ctggcgcacc gccgggtgaa ccgagacccg gctggccagc cccccggtct cctccgagcg
      241 gagtgccggg ttgccgagca ggctgtcggc aaaggccgtc gcggccagcg cgtctcccgc
      301 attgtcggga ttgccgccgt cgtgaaagca ctggtagccg accgcacgat agagcagccc
      361 ggtgtcgagg tgcggcaggc catagtggcg cgccaaggcc ttggcgatgg tgcccttgcc
      421 cgaagcggtt ggcccgtcga cggcgatgat catgcccccg gtccttcagc gccccgcgct
      481 cgcccccgcg cccgccaagc cttgaccagc ccccagatgg cgatcgaggc ccagaagaac
      541 tccagcacca ggctcgccgg gttgaggttc accagcagcg aaaccgtcag caggcctgcc
      601 ccagcgaggt tcaccccgtg ctgcacgaag gggttgggca gcttgttatg ggtcgcataa
      661 gcataggcaa agatgatgca tgccatgccg gcgaagccgc tggcattggc caaggcgtgg
      721 ctgagcggca ggatcatccg gccaatccgt cgagcaggct ttcgaacacc gggaacgagg
      781 tcgcgatcgg gcgggtgtcg tccacttcca ccccgcggtg gctcgccagc cccgccacgg
      841 ccagcgacat ggcgatccgg tgatcgaggt gagtggcgac ggcgctgccc gggggcgtgc
      901 cggccagcgg ctcgccgccg gtcccgtcga tgatcaggcc gtcctcggtc tcggtcaccc
      961 gcgccccggc aagctctagc gcggcgcgca ttacggcgat gcggtccgat tccttgaccc
     1021 gcagttcctc aaggcccgtg gttacggtcc ggcccttcgc cagcgcggcc gccacgaaca
     1081 ggatcgggaa ttcgtcgacc atcgccggca ccacggcgcg atccaccgcg atcccgctca
     1141 gcggggaatg gcgcacgtgg agatcggcca ctggctcgcc gccgacctcg cgccggtcga
     1201 cctcggcgat cgatccaccc atgtcgcgca aaacgtcgaa cagcgccgcc cgcgttggat
     1261 taaggccgac gttctcgatc accaggtccg atccgggcac cagcagggcc gccacaacga
     1321 agaaggcggc tgacgacgga tctccgggca cgacgatctg ccgcccggtc agctcggcct
     1381 cgccggtcag gcggatgatt cgcttgctgt cggcttgaac ctcgacctca aggcgggcgc
     1441 cgaacccgcg cagcatgcgc tcggaatggt cgcgggtggg gaccggctcg atcaccgtgg
     1501 tgacgcccgg cgtgttgagc ccggcgagca gcacggcgct ctttacctgc gcactggcca
     1561 ccggcagccg gtaggtgatc ggcacggcag gtgccgcacc gcgcatcatc agcggcaggc
     1621 ggccacctgg cgaggcggtg aaatcggcgc ccatcagcga gagcggctcg atcacccgcc
     1681 ccattggccg ttttgacagg ctggcatcgc cgacgaagct ggcggtgatg gcgtggctcg
     1741 cgaccaggcc catcagcagg cgggttgagg tcccggagtt gcccatgtcg agcgcggcct
     1801 cgggctggag cagcccgccc acccccacgc catggattac ccagtcaccc gttgcctcgc
     1861 gcatcacgct cgcccccatg gcgcgcatgg cagccgcggt ggagagcacg tcctccccct
     1921 cgagcaggcc cgatacccgc gtctcgccca gcgccagcgc gccgagcatg atcgagcggt
     1981 ggctgatcga cttgtcgccg ggaacgcgga tgcgcccctt cagggggccg gtggcggcaa
     2041 atcggcgcgg acgcaccgag gcgggatcgg agtggctcac aggcgggctt ccaacatggt
     2101 gatcaatcaa ccgggcggat tgatattcag tccagaacca gccgaaaatg gtggcttcaa
     2161 gtgagccgga gcgcagcgac cttagtggtc gtgagcaccg gatgcacatg gagtcgccat
     2221 ttgcaggccg tcatgggctg aatatcgatg cgcccgaagc gcgcggcttt tgacagcgcg
     2281 ggcctgctat ggcaaggcgc gcgcccggtg gatgccgtgg cgcttgcaat tcgcgccgca
     2341 ccttcccatc accgcgacgg ttttttgatg gtctgcccaa gtgggcgggc caagacgagg
     2401 aatgacaatg gtcaagcctg aatggggcgc caagcatggt tgcccgaagt gcggcacccg
     2461 tttctacgac ctgggcaagg acgacccggt cacctgcatc gaatgtggct atgcctggca
     2521 ccccgagccg gtgctgaagt cgaagcagcc gatgccttat gaggaaatcc agaagaagac
     2581 cgagacgccc gacgccgatc tggccgagga tgatctggac atcgacgagg acggcgacag
     2641 ccccgacaac gatgttgacc tcggcggaga cgacgatctc ggcgtcggcg tcgacagcga
     2701 cggcgagccc gacgacgtct gacgaatttt ttgaaaatac ccgttgccaa gttcgcgggg
     2761 cgcttttaga ggggcgctcc tcggcggcag ccgggccggt tggtccccca gggcgggccg
     2821 gaatgaaatg gtcacggggc cttagctcag ctgggagagc gcctgcatgg catgcaggag
     2881 gtcagcggtt cgatcccgct aggctccacc atttcaaacg aagtttcgcc tttgagcgat
     2941 gcgctggctg acgaggtttc gtccgccggc gtttttcgct tgagggattc gggttgatcg
     3001 ctggccgacg aggtttcgtc cgccggcgcg atttgcgttt ttggccatct ggccggtttg
     3061 gagcaggcga ctcatgttcg acacattgtc cgatcgcctc tgcggggtgt tcgacaagct
     3121 gcgcgggcgc ggtgcgctca aggagcagga cgtccgcgat gccatgcgcg aagttcgcat
     3181 cgcgctgctc gaagccgatg tcgcgctccc ggtcgttcgc cgcttcatcg accaggtgac
     3241 cgaggccgcc gtcggccagt cggttctcaa gtcagtcacc cccggccaac aggtcgtcaa
     3301 gatcgtcaat gacgcgctgg tcgagatgct cggcggcgaa gagaccgtcg gcctcgatct
     3361 caacgccgcg cccccggtcg tcatcatgat ggtcggcctc cagggctcgg gcaagacgac
     3421 gaccacggcc aagatcgcca agctgctcaa ggaaaa
//
LOCUS       DATNBV010000493         3761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_915311,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000493 DATNBV010000000
VERSION     DATNBV010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915311"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="VN627_11665"
     CDS             complement(<1..1198)
                     /locus_tag="VN627_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXD03953.1"
                     /translation="MATLLADNEPTVTTGTPTPEAVVVRFAGDSGDGMQLTGGQFTLS
                     TALAGNDLATFPDFPAEIRAPQGTLFGVSAFQINFGSTAIETAGDAPDVLVAMNPAAL
                     KTNVQALKPGGLIIADTGEFTKRNLEKAKYEVNPLEDGSLAKWDLLAFDISALTLESV
                     KPFGLGNKEALRCKNMWTLGLALWMFDRDRAPLVEWLKDKFRKNPVLADANIAALNAG
                     HAYGETAELAGPLKKLHLDPVESAPGLYRTVTGAEAVSLGLVAGAQLAELPMFFGGYP
                     ITPASAILHNLVKLKEFGVTTFQAEDEIAAICSAIGASYAGQLGVTSSSGPGIALKGE
                     AMGLAIMTELPLVIVNSQRGGPSTGLPTKTEQSDLYQAVYGRNGDAPMPVIAACSPSD
                     AFEMAVE"
     gene            1456..1938
                     /locus_tag="VN627_11670"
     CDS             1456..1938
                     /locus_tag="VN627_11670"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HXD03954.1"
                     /translation="MNDFTALPYRPCVGVMLVNGQGLVFVGKRIDTREGDFWQMPQGG
                     IDDGEEVRAAALRELWEETGVTEAHVTLLAQTREELLYDLPDELLGKLWGGRYRGQRQ
                     FWMLARFTGEDHHIVLDAHDPAEFCDWKWVRPDELPDLIVPFKKRVYRTVVDEFRDLI
                     "
     gene            complement(1984..2799)
                     /locus_tag="VN627_11675"
     CDS             complement(1984..2799)
                     /locus_tag="VN627_11675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD03955.1"
                     /translation="MEITRDTVAVITGGASGMGRSTALALARSGASVAIADLNEARIA
                     ETVAELEALGVGALGVRCDVSRPEDIANLRDRTVERFGRVDILMNNAGILPLGSISAM
                     PLAEFERAMRVNFLAVVCGVQTFLPDLAARGQAHIVNTASMAGTFAYEPDSVAYNASK
                     AAVLSLTENLALGLAPQGIGVTCLVPGGVATNIMEQVKVSGEIGDLASYVGAHFVMRT
                     ADEVGEMVVAAIRDNTFFLPTNPELVALLARRAADPDGFVHEMQDWFAAHQPA"
     gene            complement(2893..3336)
                     /locus_tag="VN627_11680"
     CDS             complement(2893..3336)
                     /locus_tag="VN627_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03956.1"
                     /translation="MSLSAPLQELDPRATALVGEGRAALAADNVEGAIDAFEAALAIQ
                     PGSTAITLDLAEATRRQGLQGKALHYYREALERDPRNVAAISGEGAALLEKGAVEKAK
                     RNLARLQTLCGTSCPEAESLAALIARTPERVASAEGAKPSPVVAN"
     gene            complement(3479..>3761)
                     /locus_tag="VN627_11685"
     CDS             complement(3479..>3761)
                     /locus_tag="VN627_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03957.1"
                     /translation="SLEPVDLVFDAAGGDAAERSAPLVRPGGRLVYVVCSLLDAEGAD
                     QVAGFLGRHPAWRAVVPPLGAGERRGDGLRLTAQFDECDGFFVARLERS"
BASE COUNT          655 a         1270 c         1221 g          615 t
ORIGIN      
        1 cctcgaccgc catctcgaac gcatcggacg gcgaacaagc cgcgatcacc ggcatcggcg
       61 catcgccgtt gcggccatag accgcctgat agagatcgga ctgctcggtc ttcgtaggca
      121 agccggtgga aggcccgccg cgttgcgaat tgacgatgac gagcggcagc tcggtcatga
      181 tcgccagccc catcgcctcg cccttgagcg cgatgcccgg gcctgacgac gaggtcaccc
      241 ccagctgccc ggcatagctt gcgccgatgg ccgagcagat cgcggcgatt tcgtcttcgg
      301 cctggaaggt ggtgacgccg aactccttga gcttcaccag gttgtggagg atcgccgagg
      361 ccggggtgat cgggtagccg ccgaagaaca tcggcagttc ggcaagctgt gcgcccgcaa
      421 ccaggccaag gctcacggct tccgcgccgg tcaccgtccg gtagagcccg ggggcgcttt
      481 ccaccggatc gaggtgcagc ttcttcagcg gcccggccaa ctcggccgtt tcgccatagg
      541 catggcccgc gttcagcgca gcgatgttgg cgtcagccag cacggggttc ttgcggaact
      601 tgtccttgag ccattcgacc agcggtgccc ggtcacgatc gaacatccac agggctaggc
      661 ccagcgtcca catgttcttg cagcgcaggg cttccttgtt gccgaggccg aagggcttca
      721 ccgattcaag ggtcagcgcg gaaatgtcga aggcgagaag gtcccacttg gcgagggagc
      781 catcctccag cgggttgacc tcgtacttgg ccttctcgag gttgcgcttg gtgaactcgc
      841 cggtgtcggc gatgatcagg ccgccgggct tgagcgcctg gacgttggtc ttcagcgccg
      901 ccgggttcat cgccaccagc acgtcggggg catcgcccgc ggtctcgatc gcggtcgagc
      961 cgaagttgat ctggaaggcc gaaacgccga acagcgtgcc ctgcggcgcg cggatttcgg
     1021 ccggaaagtc gggaaatgtc gccaggtcgt tgccggccag cgcggtggac agggtgaact
     1081 gcccgcccgt cagctgcatc ccgtcgccgg aatcgcccgc aaagcgcacg accaccgctt
     1141 cgggggtagg agtgcccgtc gtcacagtgg gctcgttatc ggccagaaga gttgccattt
     1201 tcatcctcta acgggaaatg cgtagccgga ttcgaaccgg ctttttccat gacttcgcct
     1261 aagccactct atggccccgc gcgcaattga gtttttctgt caggcccgaa acccaagggt
     1321 tttttcggcc attgtgaaga gatgggtaca agcaacggct ggtcaagccg gcgcggcgtg
     1381 cctaaggccg gctccggccg actcccaccg ctagaggccg ctgcctctgc ctcatgacgt
     1441 gagcaaaccc ttccgatgaa cgatttcact gccctgccct accgcccctg cgtcggcgtg
     1501 atgctggtca atggccaggg actggtcttt gtgggaaagc ggatcgacac ccgcgaaggc
     1561 gatttctggc agatgccgca aggtgggatc gacgatggcg aggaagtccg cgccgccgcc
     1621 ctgcgcgaac tgtgggagga aacgggcgtg accgaggctc acgtcaccct cctcgcccag
     1681 acccgcgagg aactgctcta cgacctgccc gacgaactgc tcggcaagct ctggggcggg
     1741 cgctatcgcg gccagcgcca gttttggatg ctggcccgct tcaccggcga ggatcaccat
     1801 atcgtgctgg atgcccacga tccggccgag ttctgcgact ggaaatgggt gcgcccggat
     1861 gagctgcccg acctgatcgt gccgttcaag aagcgcgtct accgcacggt cgtggatgag
     1921 ttccgcgacc tcatctgact ggcccggtgc gagcggcctg atccgaacag accgccccgc
     1981 cgctcaggcg ggttggtggg ccgcgaacca gtcctgcatt tcgtggacga acccgtcggg
     2041 atcggccgcg cgccgggcca gaagggcgac cagttccggg ttcgtaggca ggaagaaggt
     2101 attgtcgcgg attgccgcga ccaccatctc cccgacctca tcggccgtgc gcatgacgaa
     2161 gtgggcccca acatagctcg caagatcgcc gatctcgccg gaaaccttca cctgctccat
     2221 gatgttggtc gcaacgccgc cggggacgag gcaggtcacg ccaatgccct gcggggccag
     2281 ccccagagcc aggttctccg tcaggctgag caccgcagcc ttgctggcat tgtaggccac
     2341 cgagtccggc tcgtaggcga aggtcccggc catagaggcg gtgttgacga tatgggcctg
     2401 cccgcgggct gccagatcgg gcaggaaggt ctgtacgccg cagaccacgg cgaggaaatt
     2461 cacccgcatg gcccgctcga attcggccag cggcatggcc gagatcgagc cgagcgggag
     2521 aatgcccgca ttgttcatca ggatgtcgac ccggccgaac cgctcaaccg tccggtcacg
     2581 caggttggcg atgtcctcgg gccgggaaac gtcgcagcgc acacccaatg cgccgacgcc
     2641 gagagcttcg agttcggcca ccgtctcggc gatgcgtgct tcgttcagat cggcaatggc
     2701 cacgctggcc ccgctgcgag cgagcgcaag ggccgtgctg cggcccatcc cgctggcgcc
     2761 gccggtaatg acggcgaccg tgtcacgggt aatttccatg ctggcaatcc tctcgatcag
     2821 actcgcgaca ccgcgcgttg ccgcgctatc gcgcggcgcg aactgcagcg caagagtcgg
     2881 cgaaagagct ggtcagttcg ccaccaccgg cgagggcttg gccccctcgg ccgaggccac
     2941 ccgctcgggc gtgcgcgcga tcagggccgc gaggctttcc gcctcggggc aggacgttcc
     3001 gcacagggtt tgcaggcggg cgaggttacg cttggccttc tccaccgcgc ccttttccag
     3061 cagcgccgcg ccttctccgg agatcgccgc gacgttgcgc ggatcgcgtt ccagcgcctc
     3121 gcgataataa tgcagcgcct tgccctgaag gccctgccgc cgggtcgcct cggcgaggtc
     3181 gagcgtgatc gcagttgaac cgggctggat cgccagcgcc gcctcgaagg catcgatcgc
     3241 cccttcgaca ttgtcagccg ccagcgccgc gcggccttcg ccgacgagcg cggtggcgcg
     3301 gggatcgagc tcctgcagcg gggccgacag gctcatgctg gccgacaggc caagaaccag
     3361 agaaagtgcg atcccagccg gcgcaaaacg catcaggaac tcccgcggtg ccatccgata
     3421 atccaagccc caggttcccg cctgtgccgg gcagggccat aactccaccc aaccgctatc
     3481 acgaccgttc cagtctggcg acgaaaaagc cgtcgcattc atcaaattgc gcggtaagcc
     3541 gcaagccatc accgcgccgc tcccctgccc ccagcggggg tacaacagcc cgccaggcgg
     3601 gatggcggcc cagaaaaccg gcaacctgat ctgccccttc ggcatcaagc agcgaacaga
     3661 ccacatagac cagccgcccg cccgggcgca ccagcggggc ggagcgttcc gcggcatctc
     3721 cgcccgccgc atcgaagacg aggtcgaccg gctcgagcga a
//
LOCUS       DATNBV010000494         2773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4748127,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000494 DATNBV010000000
VERSION     DATNBV010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4748127"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1756)
                     /gene="hcp"
                     /locus_tag="VN627_11690"
                     /gene_synonym="priS"
     CDS             complement(116..1756)
                     /gene="hcp"
                     /locus_tag="VN627_11690"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HXD03958.1"
                     /translation="MYCVQCEQSNKGGCAVKIGSCGKTADVADMQDVLLRLMEGVSVY
                     ADRAARVGAQDAAVNAFTPHAWFTTLTNVNFDARRFDGLIAEALALRERARTLAEAAG
                     ADLSDLPEPATWNPGTTASEIAAAAPMAAIQRFMDRDGPSIVGLRNLILYGLKGTAAY
                     SEHARVLGTEEAAVSAEFHRISAFLAADPTDMNALLTEALAVGALNLKVMELLDAANT
                     GRFGHPEVTMVRMSPVKGKAILVSGHDMGDLEAILQQTAGKGINVYTHGELMPANAYP
                     GLKKFPHLVGNFGGAWQDQQKEFAAFPGAIVMTSNCLINPEIRGYQDRIFTAGPVGWN
                     GLPHIENHDFSPVIACALAQPGFAEDAAEQRIPAGFARNTVMGVADTLLGMIKEGDVK
                     NLFLIGGCDGARPGRNYFHDLAMATPKDSLILTLGCGKFRFNREDLGDIKGVPRVLDM
                     GQCNDAYSAIQVAVAVAGALGCEVNDLPLHYGISWFEQKATAVLLTMLHLGLKRIHLG
                     PTLPQFLTPEVLGVLVEKFDIRPTGDAREDLARMLEAA"
     gene            1910..2311
                     /locus_tag="VN627_11695"
     CDS             1910..2311
                     /locus_tag="VN627_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXD03959.1"
                     /translation="MQLTRHTDYALRLLIYLAGIGDGQSQIAAVAEAQDISRTHLMKI
                     ANELSRAGFIAASRGRGGGIRLARPAAEINLGAVVEAMEPRCPMVDCGSCKLIRRCSL
                     PGVLDQAGTAFREVLSRYTLADICRTPALAG"
     gene            complement(2319..>2773)
                     /locus_tag="VN627_11700"
     CDS             complement(2319..>2773)
                     /locus_tag="VN627_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXD03960.1"
                     /translation="DLGFGPMAPGLDQIYHDECARLGRKPRECRKLVGGPMSIHLCED
                     PDAGWAAIERHAVHVITEYAKWSGEDSAGATSPFAGLTDPVALRQSGLFVAMTPEQFL
                     ATAPAMIDGQTVGFWPLVGGLEPAEAKKSMGLLGQVLPEFRARVAAQG"
BASE COUNT          449 a          921 c          935 g          468 t
ORIGIN      
        1 aaaagcagga gccccttcac ccgcgatgag ggctgcgggt gaaggggctc tccccatccg
       61 gcgccgaggg gcggcgccgg cgggttgtgg aagcgggctg accggtgagg ggtggtcagg
      121 cggcttccag catccgcgcc aggtcctcgc gggcatcgcc ggtcggacgg atgtcgaact
      181 tctcgaccag cacgccgagc acctcgggcg tgaggaactg cggcagcgtg ggccccaggt
      241 gaatgcgctt gagaccgagg tgcagcatgg tcagcaggac ggccgtggcc ttctgctcga
      301 accagctgat cccgtagtgc agcggcaggt cgttgacctc gcagcccagc gccccggcga
      361 cggcgaccgc cacctggatc gccgaatagg catcgttgca ctggcccatg tcgagcaccc
      421 ggggcacgcc cttgatgtcg cccaggtcct cacggttgaa gcggaacttg ccgcagccca
      481 gggtcaagat caggctgtcc ttcggcgtcg ccatggccag gtcatggaag tagttacgcc
      541 ccggccgcgc cccgtcgcag ccgccgatca ggaacaggtt cttgacgtcc ccctccttga
      601 tcatgcctag cagggtatcg gccacgccca tcaccgtgtt gcgggcaaag ccggcgggga
      661 tgcgctgttc cgccgcgtcc tcggcgaagc ccggctgggc aagggcgcag gcgatcacgg
      721 gcgagaaatc gtggttctcg atgtgcggca ggccgttcca gccgaccggc ccggcggtga
      781 aaatgcggtc ctggtaaccc ctgatctccg ggttgatcag gcagttggag gtcatcacga
      841 tcgcgccggg gaaggcggcg aactccttct gctggtcctg ccaggctcca ccaaaattgc
      901 cgacaaggtg gggaaacttc ttgaggcccg gataggcatt ggccggcatc agctcgccat
      961 gggtatagac gttgatcccc ttgcccgcgg tctgctggag gatcgcctcg aggtcgccca
     1021 tgtcgtggcc ggagacgagg atcgccttgc ccttgaccgg gctcatgcgc accattgtca
     1081 cctccggatg gccgaaccgc ccggtattgg ccgcgtcgag cagttccatg accttgaggt
     1141 tgagcgcgcc caccgccagc gcctcggtca gcagcgcgtt catgtcggtg ggatcggcgg
     1201 ctaggaaagc cgagatgcga tggaattcgg cgctcaccgc cgcttcctcc gtgcccagca
     1261 ctcgggcatg ctcggaatag gcggccgtgc ccttgaggcc gtagaggatc aggttgcgca
     1321 ggcccacgat cgaggggccg tcacggtcca tgaaccgctg gatcgcggcc atcggcgcgg
     1381 ccgcggcaat ttccgatgcg gtcgtgccgg ggttccacgt agctggttcc ggcaggtcgg
     1441 acaggtcggc tccggccgct tccgccagcg tgcgagcccg ttcgcgcaag gccagcgcct
     1501 cggcgatcag cccgtcgaaa cggcgggcgt cgaagttcac gttggtgagg gtggtgaacc
     1561 aggcgtgggg tgtaaaggca ttcaccgcgg cgtcctgcgc gcccacccgt gccgcgcggt
     1621 cggcatagac cgagacgcct tccatcaggc gcagcagcac gtcctgcatg tctgccacgt
     1681 cagcggtctt tccgcaggag ccgattttca cggcacagcc gcccttgttc gattgttcgc
     1741 attgcacgca atacatggag gcactccgat aaagatgaag agtgaattcc acttatgggc
     1801 cggcggcaaa gatagctttg atctgaatca attcccttcc tgacattccg gcagataagg
     1861 gtattgcccg gaggcgccgt cagctaggat agtgcggggg ccagatgcca tgcagctcac
     1921 gcgacacact gactatgccc tgcgcctgct gatctacctc gccgggatcg gcgatggaca
     1981 atcgcagatc gccgccgtgg ccgaagcgca ggatatctcg cgcacgcacc tgatgaagat
     2041 cgccaacgag ctgtcgcgcg ccggcttcat cgccgcctcg cgcgggcgtg ggggcggcat
     2101 tcgcctggcg cgcccggcag ccgagatcaa cctcggcgcc gtggtcgagg caatggagcc
     2161 gcgctgcccg atggtcgatt gcggatcgtg caaactgatc cggcgctgca gcctgcccgg
     2221 tgttctcgac caggccggca cggcgttccg cgaagtgctg tcgcgctaca ccctggccga
     2281 catctgccgg acgccggcgc tcgccggctg aaccgcgctc acccctgcgc ggccacgcgc
     2341 gcccgaaatt ccggcaggac ctgtcccagc agacccatgc tcttcttcgc ctcggccggc
     2401 tcgagcccgc cgaccagggg ccagaatccg acagtctgac catcgatcat ggctggcgcc
     2461 gtggcgagga attgctcggg cgtcatggcc acgaacagtc cggactggcg cagcgccacc
     2521 gggtcggtca gtccggcgaa gggcgaggtc gctccggcgc tgtcctcccc cgaccacttg
     2581 gcatattcgg tgatcacgtg gacggcatgg cgctcgatcg cggcccagcc ggcatcgggg
     2641 tcctcgcaca gatggatcga catcggcccg ccgacgagct tccggcattc cctgggtttc
     2701 cggcccaggc gggcgcactc gtcgtggtag atctggtcga gcccgggtgc catgggcccg
     2761 aacccgaggt cga
//
LOCUS       DATNBV010000495         3341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3483158,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000495 DATNBV010000000
VERSION     DATNBV010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3483158"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..393)
                     /locus_tag="VN627_11705"
     CDS             complement(10..393)
                     /locus_tag="VN627_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXD03961.1"
                     /translation="MLLAVQTVPMAQAQTQPQTQGDVTYVSGGIADDWQQAMEAQKGQ
                     YNLHLLFAQEGTGAYFTNVPVQITDSSGQTVLNAVSQGPFFYARLKPGSYKVTASHGG
                     QAVTKSATVSASGGADLDYRWAGGN"
     gene            complement(532..789)
                     /locus_tag="VN627_11710"
                     /pseudo
     CDS             complement(532..789)
                     /locus_tag="VN627_11710"
                     /inference="COORDINATES: protein motif:HMM:NF037969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
     gene            complement(1131..1421)
                     /locus_tag="VN627_11715"
     CDS             complement(1131..1421)
                     /locus_tag="VN627_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03962.1"
                     /translation="MTIKRYSRLFLLAALLGAVFAVLPTKVDHGELFGISAALAAHTG
                     GGNGRNGGMGHESTASGNAGTSAGTEASHDVAHGVDPDRARDQRNCDLVSCE"
     gene            1705..2664
                     /locus_tag="VN627_11720"
     CDS             1705..2664
                     /locus_tag="VN627_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXD03963.1"
                     /translation="MEDTGPGVLDIGRIDLPPLESEAPPPALADGPRVEMTVTPRAAA
                     SRRRADWRGLAGEVVQFAGAAPFEYRFQGQVHLLTSYERAIRSAGETWIDGRQVSTRH
                     DFSRTLSFIPQGHQLKGSFVPRVLPLSSYFYIDPARLPADPELRFAETDFAPMLFFED
                     PALWTTTRKLMGLIESPGAERLYAETLGALLAVELMRAHQGVGMAPPADRGGLAAWQQ
                     RIACDYIEANLDRDIGLESLAALVRLSPTHFCRAFRRSLGMPPHRYHIHRRLEHAKTL
                     LADRERSITTVAMVSGFGASSSFATAFRKATGLTPQQYRRSLF"
     gene            complement(2679..>3341)
                     /locus_tag="VN627_11725"
     CDS             complement(2679..>3341)
                     /locus_tag="VN627_11725"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HXD03964.1"
                     /translation="CADHGDMLGERGLWYKMAPYERSTRIPLIVSTPDAKGGRRVSAN
                     VSLIDLLPTVLDLAGEGGSLDPIEPLDGRSLVPFLTGGGEGWGDVAVSELFFEGLAEP
                     ALMLRKGRYKYIHCNRETQLLFDIESDPHELHDLAGDPAHGAKLAELSGLVARRWDFD
                     RLTREILLSQRRRNLIQRSVLAGRYPAWDFQPFEDATRLYYRGDGGDWHAAEQRDFLR
                     FK"
BASE COUNT          566 a         1135 c         1077 g          563 t
ORIGIN      
        1 aaaggcagcc taattgccgc cggcccagcg gtagtcgaga tcggcaccgc cgcttgccga
       61 cacggtggcc gacttcgtca cggcctgccc gccatggctg gcggtcacct tgtagctacc
      121 cggcttcaac cgggcataga agaacgggcc ctgggagacg gcgttcagca cggtctggcc
      181 gctgctgtca gtgatctgca ccggcacatt ggtgaaataa gcgccggtcc cctcctgggc
      241 gaagagaagg tgcagattgt actggccctt ctgcgcctcc atggcctgct gccaatcatc
      301 agcgatgccc cccgagacat aggtgacgtc accctgggtc tggggctgcg tctgcgcctg
      361 agccatcggc acggtctgca cggccaagag cacggcgaga gagacggacg cggaaatcct
      421 gcgcatggga ttaattctcc aaaggatgac ggccggtaat attcggctta gatccacaac
      481 gtgggattcg gtgcacggtt ccgcaacagc cccggtaacg ccgagggact cctaaaaggt
      541 caggccggcg tgaaccagaa taccgacggc gacgagccca tacatgatca cgccgatggt
      601 gttgccgatg atcaggggga gctcgcgccg gtggatccca taggccaccc agagcagggc
      661 gatggtcgta tagacggccc aggtgatcag ggactgcccc gcggcatgct ggctatgggt
      721 cgcaaagacc ttgatgactt gcggaatggt ggcgaggggg ctgataaggc cgatcaccac
      781 catcaggtgg tggaatttcg atcgctttta ccgtgccatc ccctgccccc aggtccgccg
      841 ccccggcggg tttacctcgc ctgttttgct cgatcagggc cggtttgtcg actcatccga
      901 gcagcaggcg ggcggctgtg accagcgtga cggcgatcca aaatcccagg agaaggtgct
      961 gttcgtttcg gggcatgggt tgcatcccgc attattcccg ccccccatca tctgcccccg
     1021 ggccataagg ctccagacgg ttccacgccg aacgggataa gcggcccata agaaacggcg
     1081 cgctgcgacc ggccgttcat gccggcggcc gcgggcgcgc cgttctcggg tcactcgcag
     1141 gaaaccaggt cgcaattccg ctgatcgcgc gcacggtcgg gatcgacacc atgggcgacg
     1201 tcatggcttg cctccgtgcc ggcgctcgtc ccggcattgc cgctggcggt cgactcatgg
     1261 cccatgccgc cgttcctgcc attgccgccg cccgtgtgag ccgccagggc cgcgctgatg
     1321 ccgaacaact ctccgtgatc caccttggtg ggcagaaccg cgaagaccgc gcccagaagt
     1381 gccgcgagaa ggaaaagcct cgaataccgc ttgatggtca tgacgagcct cgtgctgtga
     1441 agcgatcggc ctgggggtgc agatcccgct gggcctgacc agcatgtagg ccgtcgcgcg
     1501 aaagccggtt ttgcctccct tagggggatt tcagggattc tggcgcaatc gatctgcccg
     1561 ggcgcccggc gtccacggcc aggttgctat gcaacatcgg ctgacagcca tgacgaaacc
     1621 tgcgttgtcc cgcagggcgc ttcggtccaa ttcccctcta ctgcgttgca gcatccacag
     1681 accttggggg tcaaggagca gacgatggaa gacacaggcc ccggagtatt ggacatcggg
     1741 cggatcgacc tcccccctct ggaatcggag gccccgccgc cggccctggc ggacgggccc
     1801 cgggtggaga tgacggtcac ccctcgggct gcggcaagcc gccgccgggc cgattggcgg
     1861 ggtctggccg gcgaggtcgt ccagttcgcc ggggccgcac ccttcgaata ccgcttccag
     1921 ggccaggtcc atctcctgac cagctatgag agggccatcc ggagcgccgg cgagacctgg
     1981 atcgacggaa ggcaggtctc cacgcgccat gacttcagcc ggaccctgtc cttcataccc
     2041 cagggccacc agctgaaggg aagcttcgtc ccgcgcgtcc tgcccctcag cagttacttc
     2101 tatatcgatc ccgcccggct gcccgccgat ccggagctgc gcttcgccga gaccgacttc
     2161 gcgcccatgc tgttcttcga ggatccggcg ctctggacca cgacgcgcaa gctcatgggg
     2221 ctgatcgaga gtccgggcgc ggaacgtctc tatgccgaga ccctgggtgc cctgctggcg
     2281 gtcgagctca tgcgcgccca tcagggcgtg ggcatggcgc cgccggccga tcgcggcggg
     2341 ctcgcggcct ggcagcagcg gatcgcctgc gactatatcg aggccaatct cgatcgggat
     2401 atcgggctgg agtccctggc cgcgctcgtc cgcctgagcc cgacccattt ctgccgcgcc
     2461 ttccgccgct ccctcggcat gccgccgcat cgctatcaca tccaccggcg gctcgagcat
     2521 gccaagaccc tgctagccga tcgcgagcgc tcgatcacga cggtggcaat ggtgagcggc
     2581 ttcggcgcct ccagcagctt cgccaccgcc ttccgcaagg ccacggggct gacgccgcag
     2641 caatatcgcc ggagcttgtt ctaggtcatt ttctcccctc acttgaaccg caggaaatcg
     2701 cgctgctccg cggcgtgcca atcgccgccg tcgccgcggt agtagagccg cgtcgcatcc
     2761 tcgaagggct ggaagtccca ggccgggtag cgcccggcca gcacgctgcg ctggatcaga
     2821 ttgcgacggc gctggctgag caggatctca cgcgtcaacc ggtcgaaatc ccagcggcgc
     2881 gccaccagcc cgctgagctc cgccagcttg gccccatgcg ccgggtcgcc ggccagatcg
     2941 tggagctcat ggggatcgct ctcgatatcg aagagcaact gggtctcccg gttgcaatgg
     3001 atgtatttgt agcggccctt gcgcagcatc agcgccggct ccgccagccc ctcgaagaag
     3061 agctcgctca ctgcgacatc cccccagccc tcgccaccgc cggtgaggaa ggggacgagg
     3121 ctgcgcccgt cgaggggctc gatcgggtcg agcgagccgc cctcgccggc gagatccagg
     3181 accgtcggca gcagatcgat gagcgagaca ttggcggaga cccgccgccc gcccttggcg
     3241 tcgggcgtcg agacgatcag cgggatgcgc gtcgaccgct catagggcgc catcttgtac
     3301 cagagcccgc gttcgcccag catatcgccg tgatcggcgc a
//
LOCUS       DATNBV010000496         2956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_290765,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000496 DATNBV010000000
VERSION     DATNBV010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290765"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..503)
                     /locus_tag="VN627_11730"
     CDS             complement(39..503)
                     /locus_tag="VN627_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HXD03965.1"
                     /translation="MADLPAPYVEPEHRAAIARIAASYLALTGRDLIDPRAEPAMALW
                     EAPLAIVAHGTEADPLFFFASKVALERFGMTPARFLGLPSRFSAEAPLREERQALLDR
                     VSRHGFIDDYAGVRIAADGRRFRIEAATVWNLVDPDGSVHGQAAAFSRWTDL"
     regulatory      698..813
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            846..2711
                     /gene="thiC"
                     /locus_tag="VN627_11735"
     CDS             846..2711
                     /gene="thiC"
                     /locus_tag="VN627_11735"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HXD03966.1"
                     /translation="MADINSKLEIGVTTGPIRGSRKIHVESPRLPGVKVAMREISLEP
                     GSGELPVRVYDTSGPYTDPAAQIDISAGLPQLRREWIMARGDVEEYDARAVRPEDNGL
                     KGPDRSAGVPGFPNVVQRPLRAKAGQNVSQMHYARRGIITPEMEYVAIRENLGRQALK
                     DQLIRDGQDWGASIPDHVTAEFVRDEVARGRAIIPNNINHPESEPMAIGRNFLVKINA
                     NIGNSAVASDVAQEVEKMVWSIRWGADTVMDLSTGRNIHDTREWILRNSPVPIGTVPI
                     YQALEKVGGIAEDLTWEVFRDTLIEQAEQGVDYFTIHAGVRLPYIPLTAKRVTGIVSR
                     GGSIMAKWCLAHHKESFLYEHFDEITEIMKAYDIAYSLGDGLRPGSIADANDEAQFAE
                     LYTLGELTKRAWQQDVQVMIEGPGHVPMHKIKENMDKQLEACGEAPFYTLGPLVTDIA
                     PGYDHITSGIGAAMIGWYGTAMLCYVTPKEHLGLPDRDDVKVGVVTYKLAAHAADLAK
                     GHPAAKLRDDALSRARFEFRWRDQFNLSLDPDTAEQYHDQTLPAEGAKTAHFCSMCGP
                     KFCSMKITQEVRDFAAKQNAGIETFVAAEEAEEGMAHMSEVYREKGSELYLPAAE"
     gene            2724..>2956
                     /locus_tag="VN627_11740"
     CDS             2724..>2956
                     /locus_tag="VN627_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03967.1"
                     /translation="MLAALLLLMTAPPRVPVPRPEPAAMPSPAAAPAPAGAPVVGGFA
                     PMSRFAPELHKPFRAAVAAITPARHPGAHLLAA"
BASE COUNT          510 a         1089 c          933 g          424 t
ORIGIN      
        1 taggcgggag gggctggggg tgggcaagcg ccgggcgcct acaggtccgt ccaccggctg
       61 aacgccgccg cctgaccatg aaccgaaccg tccgggtcga cgaggttcca caccgtcgcc
      121 gcctcgatcc ggaagcgccg gccgtcggcg gcgatccgca ccccggcata gtcgtcgatg
      181 aagccatggc ggctcacccg gtcaagcagg gcctgccgct cctcacgcag gggcgcttcg
      241 gccgagaacc ggctgggcag cccgaggaac cgggccggcg tcatcccgaa ccgctccagc
      301 gccaccttgc tggcaaagaa gaacagcggg tcggcctcgg tgccatgggc cacgatcgcc
      361 aggggcgcct cccacagggc cattgccggt tcggcacgag ggtcgatcag gtcccgcccg
      421 gtcagggcca ggtaactggc cgcaatccgg gcgatcgccg cgcggtgctc gggttccacg
      481 tagggggcgg gcagatcggc catggcgcac ccctgcgcgc gtccactccg ccgcgcaagg
      541 gcacgatcga cttgaatgcc gtcgggaact ctcctctggt ccctcccgcc tgccggtttg
      601 cccctcgccg ccttggcatg gctcggccgg ttgattacgc tttcgtcgca atggcgcagg
      661 gcagggcttc ccttccccgg cgcacatccc tactatcgcg ccatccccgg ggagcgcgcc
      721 gttcacaatg cgctgagagg ggcggatggc accgcccgac ccgtcgaacc tgaacccgtt
      781 agcacgggcg gagggagtgg tctggtcgcc accagtcgcc ccgtcgtcca gtattggagc
      841 gcatcatggc ggacatcaat tccaagctcg agatcggcgt caccaccggc ccgatccgcg
      901 gtagccgcaa gatccacgtc gaaagcccgc gcctgcccgg cgtcaaggtc gccatgcgcg
      961 agatttcgct cgaacccggc tcgggcgagc tcccggtacg ggtctatgac acgtccggcc
     1021 cctataccga ccccgccgcc cagatcgaca tttccgccgg cctgccccag ctgcgccgcg
     1081 aatggatcat ggcgcgtgga gacgtcgagg aatacgatgc ccgcgcggtc aggcccgagg
     1141 acaacggcct caaggggcct gaccgttcag ccggcgtgcc cggcttcccc aacgtggtcc
     1201 agcgcccgct gcgcgcgaag gccggccaga acgtcagcca gatgcactat gcccgccgag
     1261 gcatcatcac gcccgagatg gaatatgtcg ccatccgcga aaacctcggc cggcaggcgc
     1321 tgaaggacca gttgatccgc gacgggcagg actggggcgc gtcgatcccc gatcacgtca
     1381 ccgccgaatt cgtccgcgac gaggtcgccc gcggccgcgc catcatcccc aacaacatca
     1441 accaccccga atccgagccc atggccatcg gccgcaactt cctcgtcaag atcaacgcca
     1501 acatcggcaa ttccgccgtg gcgtccgacg tcgcgcagga ggtcgagaag atggtctggt
     1561 cgatccgctg gggggcggac acggtgatgg acctctccac cggccgcaac attcatgaca
     1621 cccgcgaatg gatcctgcgc aactcgcccg tccccatcgg caccgtgccg atctaccagg
     1681 cgctggaaaa ggtcggcggc attgccgagg atctcacctg ggaggtcttc cgcgataccc
     1741 tgatcgagca ggccgagcag ggggtggact atttcaccat ccacgccggc gtgcgcctgc
     1801 cctacatccc gctcaccgcc aagcgcgtga ccgggatcgt ttcgcgcggg ggttcgatca
     1861 tggccaagtg gtgcctggcg catcacaagg agagcttcct ctacgagcac ttcgacgaga
     1921 tcaccgagat catgaaggcc tatgacatcg cctattccct cggcgatggc ctgcgccccg
     1981 gctcgattgc cgatgcgaac gacgaagcgc agttcgccga gctctacacc ctgggtgaac
     2041 tcaccaagcg cgcctggcag caggacgtcc aggtgatgat cgaaggcccc ggccacgtgc
     2101 cgatgcacaa gatcaaggag aacatggaca agcagctcga agcctgcggc gaggcgccgt
     2161 tctacaccct cgggcccctc gtcaccgata tcgcgcccgg ctacgatcac atcaccagcg
     2221 gcatcggcgc cgccatgatc gggtggtacg gcacggccat gctttgctac gtgacgccga
     2281 aggagcacct cggcctgccg gaccgcgacg acgtcaaggt cggcgttgtc acctacaagc
     2341 tcgccgccca cgcggcggac ctcgccaagg gccaccccgc cgccaagctg cgcgacgatg
     2401 ccctgagccg ggcgcggttc gagttccgct ggcgcgacca gttcaacctc agcctcgatc
     2461 ccgatacggc cgagcagtat cacgaccaga ccctgcccgc cgaaggcgcc aagaccgcgc
     2521 acttctgctc gatgtgcggc cccaagttct gctcgatgaa gatcacgcag gaagtgcgcg
     2581 acttcgcggc caagcagaac gccgggatcg aaaccttcgt cgccgccgag gaggcggagg
     2641 aaggcatggc ccacatgagc gaggtctacc gcgaaaaggg cagcgagctc tacctgcccg
     2701 ccgcggagta gcatgcaagc aggatgctgg ccgcccttct cctcctcatg acggcgccgc
     2761 cgcgcgttcc cgtgccccgg ccggaacccg cggcgatgcc gtcccccgcc gcggcccccg
     2821 ctccggccgg cgcgccggtg gtgggcggct ttgcgccgat gagccgcttc gcccccgaac
     2881 tgcacaagcc gttccgcgcc gccgtcgccg cgatcacccc ggcgcggcac cccggcgccc
     2941 atctcctcgc cgccca
//
LOCUS       DATNBV010000497         9048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_916303,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000497 DATNBV010000000
VERSION     DATNBV010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916303"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VN627_11745"
     CDS             <1..854
                     /locus_tag="VN627_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXD03968.1"
                     /translation="FAMPLPARLMCLMLGALEAEWKSYKAWADQYFFNMYNDNQFDGQ
                     ARTTGTLEITASLFAAMSERRALLEEKGLAPDLSLLGTVLPNDFMSRFICEQIDGTPF
                     SPAEVLSMMMGIIIGGNETTMNLIANLVWRLLEVPSRWEAIKANPDLIEAAIEESLRL
                     DPPVLGMFRTPNEDIVLQGETIPKDHKVMYNIAAVNRDPAIWEDPEEFRLDRSLSSLR
                     KHVAFSGGHHMCLGAPLARMEVKRVMELLIARLPNLRIDGKDTRAPGMNVHGRVHMPV
                     RWDPVTP"
     gene            complement(881..1756)
                     /locus_tag="VN627_11750"
     CDS             complement(881..1756)
                     /locus_tag="VN627_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXD03969.1"
                     /translation="MYTTLTTHLEERILTVTLNRPDRLNAFTVEMANELETLFRAASG
                     DDAVGAIIVTGEGRAFCAGMELGLDGNAFGLNEKLRPDLAEVRQRYDDPEIRDGVRDT
                     GGRVTLAIHDCRKPVIAAIHGAAVGIGVTMTLAMDFRLATADARLGFVFGKLGIVPEA
                     CSSWFLPRLVGLQTALEWVLTADLIAADEAFAHGLLRSVHADRAEMLAEAHKLARRII
                     TGRSPVAIAFARDMLRRNPAMDEPRDAHMIESLAMFYTSIGDGREGVAAFREKRDPVF
                     SGKASQMPPPCPWWE"
     gene            complement(1767..2507)
                     /locus_tag="VN627_11755"
     CDS             complement(1767..2507)
                     /locus_tag="VN627_11755"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXD03970.1"
                     /translation="MDNTAVIRRRRVLIVHAHSEADSFVAAMRDRALQTLEQQGWEVD
                     QSDLYAMGFNPVLSPDDFGPRSSSDHLVYALEQRNAYAAGTLAPDITEEIRKVLSADL
                     LVFTFPVFWFSVPAILKGWIDRVFISGPFYGGRRIYGAGGLSGKRALAAFSLGGREHM
                     FGPGSLHGQLETGMLRHFLQGTLGYVGLDVVEPFVAYHLPYISADARTELLDRLEQRL
                     ARIDHEPTLAMPDLAGFDPSFAPLPANA"
     gene            2764..3267
                     /locus_tag="VN627_11760"
     CDS             2764..3267
                     /locus_tag="VN627_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD03971.1"
                     /translation="MTMTLDEMAARLAALEDKEAIRTLKARYLRACDLKQPDAVRDCF
                     APGEIRIAYQNFPEFTDREAFVETYRAMGCQPGVYDMHHATNWEIDLLGPDRAAGKWS
                     LNFRTILAGPRQVIRLVVEYDDVYERRDGRWWIVETVSRVTSMLMEQVGEDGNITVGV
                     LAEPPVA"
     gene            3327..4082
                     /locus_tag="VN627_11765"
     CDS             3327..4082
                     /locus_tag="VN627_11765"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXD03972.1"
                     /translation="MIDYGLGGKVALVTGAGGGIGRAAALVFARSGASVLVSDINQAG
                     GEETVGMIESAGGKALFQRCDVARSEEVKAMVAAVVAAFGRLDCAFNNAGINQVGDDE
                     YDDAIWERNIGINLSGVMRCMREEAEVMLAQGGGAIVNTASINGLVGNPAQPAYVAAK
                     HGVVGLARHGALRWAQAGIRVNAVCPGIIETPMTAPLVTDPQMKQLMDSMTPMGRMGK
                     AQEVAEAVVWLCSPAASFVTGHAMVVDGGATAV"
     gene            complement(4097..4969)
                     /locus_tag="VN627_11770"
     CDS             complement(4097..4969)
                     /locus_tag="VN627_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD03973.1"
                     /translation="MTQQKVALVTGASRGAGAGIARGFGEMGYTVYVTGRTVTPGDTK
                     GWDGSVLPGTVAETAQRVTENGGKGIPVVCDHADDASVARLFDQIRTEQGRLDVLVNN
                     AAYIHDQLIEKRPFWEKDLDAQKILDVGLRSAYVASWHAARIMVPQGSGIIAMTSSFG
                     ATCYMHGPAYGAQKAGLDKLAHDMEHDFRGTGVAAVSLWLGPQVTERSQISARTHPEQ
                     YEAFLAMAENPEFSAHVIDAIDRAPNRDELSGQTLIVAEIARELGVTDRGQDRPSHRD
                     MLGSPRPKNPAAVY"
     gene            complement(4983..5840)
                     /locus_tag="VN627_11775"
     CDS             complement(4983..5840)
                     /locus_tag="VN627_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXD03974.1"
                     /translation="MTDYTPTAIVQAHTPLGRAEIVEWRWPAILDFTMREEALMVEMS
                     LPPTSADASACFPELDAVRRCFMGTLFIRWPGVLVSGRSEAGHIRVVRCVFDPERTRA
                     LTALRADPDLDFLQAMLAIGNETLRAIMNLLRRELENPDGHSDAAVAALVELVAVEAE
                     RVITREASASISGRLAPWQFRRIRERIAQPGPVPCAAELAALCGISLRHLHRQFHALT
                     GRSVSGYIEAARIQQAKEMLQGSAQPIKAVAQACGFSHANSFTRAFRRTTGQTPLAFR
                     QSSGTHTHH"
     gene            complement(5852..6595)
                     /locus_tag="VN627_11780"
     CDS             complement(5852..6595)
                     /locus_tag="VN627_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HXD03975.1"
                     /translation="MEKVIAALWAPEGEDHAAYGARILSALPAALAAAGAQKIRLNVR
                     DAAVAAGEGLIQRWQAPQQAAVAQFWMPSANARFRGAVDSALAAHSASFAAWLVCEST
                     IIANTGYPVTPNSIGPEQASGKAAPSRTWGWSQASFISFRPDLSRQEAIAHWHSHHTR
                     VAIETQSNFEYVQNIIVRPLTDGAPAYDAFVEECFPLEALTVPEVFFDAVDQPERFAA
                     NTAAMLESCTGFIDFSRIDIIPTSQFDFA"
     gene            complement(6608..7369)
                     /locus_tag="VN627_11785"
     CDS             complement(6608..7369)
                     /locus_tag="VN627_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD03976.1"
                     /translation="MTDLSGKVALITGAGQGIGQGVALAMASAGATVVVTGRTLSKVE
                     QTAQLVAERGGTALPLACDVKSADDLAAAVEAAVNQFGGLDILVNNAQEVPLGKLDDV
                     TDEAFTAGFVSGPLAAMRLMKLARPHMAARGGGTIFNFCSSAGIRWDMSGYGAYGAVK
                     QAIRALTRAAAAEWGSEGIRVLTIAPHAASPGLMWWIENNPEEAEAFFRTIPLGRVGE
                     LEQDIGRALVALCGQDLAYLTGATIPLDGGQANFD"
     gene            complement(7399..8214)
                     /locus_tag="VN627_11790"
     CDS             complement(7399..8214)
                     /locus_tag="VN627_11790"
                     /inference="COORDINATES: protein motif:HMM:NF016879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HXD03977.1"
                     /translation="MAVTMPLCDSAAGSSPWLDDARVRDWVAFHTLRRNNGPQSLIEG
                     LPDMVETVDQLRNRERIEALIKAERRTNKALDRWFDERFISTYTIDDLAANPAGSIGR
                     LLHDHMVALGLTLELLPQRHADPSWAPASDLDYYTLRSGQTHDFDHLIGECGFDAIGE
                     VFPAGLRTANLFAHVSPELAGELMTTTMFVTFPWAMRTMLHYPQIWPVVWDNFTHGYE
                     VGSKSPNLFIVRWEELMHLTPSQVRAELGVKGFTGPRDNRAASLVFGEGREIF"
     gene            complement(8220..>9048)
                     /locus_tag="VN627_11795"
     CDS             complement(8220..>9048)
                     /locus_tag="VN627_11795"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXD03978.1"
                     /translation="AIRWYEAQNYAPLPGDEPGNPQARRAYAEAMISGWLNLSPELAS
                     IHPLDPDAPDEEIIILGQLFITTMVEAMTFLPSFAEWLNGYDQSQGYEDLKTILKYLQ
                     WQDPTRRGKKWILKSPQNLPYTDIIAKAFPQAVLVMTHRDPLEVVPSYVSMEAALYKL
                     NSVHSDEAVGGFWFPRLAEWMKRFEASRARIGEDRFIDIDYREVAREPLKQAERVLAR
                     IGIPLDAAIEAALTEFMAGNKREQRPLHDYSLERFGLNEADVREAFASYRARYITKE"
BASE COUNT         1555 a         2867 c         3091 g         1535 t
ORIGIN      
        1 tgttcgccat gccgctcccc gccaggctga tgtgcctgat gctgggtgcg ctggaagccg
       61 agtggaaaag ctacaaggcc tgggccgacc agtatttctt caacatgtac aacgacaacc
      121 agttcgacgg ccaggctcga accaccggta ccctcgagat caccgcctcg ctgtttgcgg
      181 cgatgagcga gcgccgggcg ctgctcgagg agaaagggtt ggccccggac ctctcgctgc
      241 ttggcaccgt gttgccgaac gatttcatgt cgcgcttcat ctgcgagcag atcgacggga
      301 ccccgttctc gcctgccgag gtgctgtcga tgatgatggg gataatcatc ggtggcaacg
      361 agaccaccat gaacctgatc gccaacctgg tctggcgcct gctggaggtg ccgtcgcgct
      421 gggaggcgat caaggccaac cccgatctga tcgaggcagc cattgaggaa tcgctgcggc
      481 tcgacccgcc ggtcctcggc atgttccgga cccccaatga agacatcgtc ctgcagggcg
      541 agaccatccc caaggaccac aaggtgatgt acaacatcgc cgccgtgaac cgcgatcccg
      601 cgatctggga agaccccgag gagttccggc ttgaccgctc cttgtcgagc ctgcgcaagc
      661 acgtggcctt ttccggcggc caccacatgt gcctgggcgc tccgctggcg cggatggagg
      721 tcaagcgcgt gatggaactg ctgatcgcgc gcctgcccaa cctgcggatc gacggcaagg
      781 atacccgcgc gccgggaatg aacgtccatg gccgggtgca catgccggtg cgctgggacc
      841 cggttactcc ataatgttgc aaacgggacc catgtagagg ttactcccac caggggcagg
      901 gcggcggcat ttgcgacgcc ttgcccgaga agaccggatc gcgcttttcc cggaacgcgg
      961 ccacgccttc ccggccgtcg ccgatgctgg tgtagaacat ggcgagggat tcgatcatgt
     1021 gcgcgtcgcg cggctcgtcc atggcgggat tgcggcgcag catgtcccgg gcaaaagcga
     1081 ttgccaccgg ggaccggccg gtgatgatgc ggcgggccag cttgtgcgct tcggccagca
     1141 tttcggcgcg gtcggcgtgg accgaccgca gaagcccgtg cgcgaaagct tcgtcggcgg
     1201 cgatgagatc ggcggtcagg acccattcca gtgcggtctg caggccgacc aggcggggca
     1261 gaaaccagga cgagcaggct tccggaacga tgccgagctt gccgaatacg aagcccaggc
     1321 gggcatcggc agtggccagc ctgaagtcca tggcgagggt catcgtcacc ccgatgccaa
     1381 cagccgcgcc gtggatggca gcgatcaccg gtttgcggca atcgtgaatg gccagagtga
     1441 cccggccccc ggtgtcgcgc acgccatcgc ggatctcggg atcgtcgtag cgttgccgca
     1501 cttcggcgag gtccgggcgc agcttttcat tcaggccaaa ggcattgccg tcgaggccga
     1561 gctccatgcc ggcgcagaag gcccgccctt cgcccgtgac gatgatggcg ccgaccgcgt
     1621 cgtcaccgga ggcggcacga aacagcgtct ccagttcgtt tgccatctcc accgtgaagg
     1681 cgttcagccg atcggggcgg ttgagcgtca cagtcaggat gcgctcttcc agatgggtcg
     1741 tcagtgtcgt gtacatggca gctccgtcag gcgtttgccg gaagtggcgc aaaggatgga
     1801 tcaaacccgg cgaggtcggg catggccagc gtgggctcgt ggtcgatccg ggccagccgc
     1861 tgctccagcc ggtcgagcag ctccgtgcgt gcgtcggcgc tgatgtaggg gaggtgatag
     1921 gcgacgaagg gttccaccac gtccagcccc acatagccga gcgttccctg gaggaagtgc
     1981 cgcaacatgc ccgtttccag ctggccgtgc agcgagcccg ggccgaacat gtgctctcgc
     2041 ccgccgaggc tgaaggcagc cagcgcccgt tttccgctca gccccccggc gccatagatg
     2101 cgccgaccgc catagaacgg gccggagatg aatacccggt cgatccaccc tttcaggatt
     2161 gccggaacgc tgaaccagaa gaccgggaag gtgaagacga ggaggtccgc ggagagaacc
     2221 ttgcggatct cttccgtaat gtctggcgcg agggttccag cggcataggc gttgcgctgc
     2281 tccagcgcat agaccaggtg atccgaactg ctgcgcgggc cgaaatcgtc gggcgacagc
     2341 acggggttga agcccatggc atagaggtcg gactggtcca cttcccagcc ttgctgctcc
     2401 agcgtctgca gcgcgcggtc ccgcatcgcg gcaacgaacg aatccgcttc cgagtgagcg
     2461 tgaacgatca gcaccctgcg gcgccggatg actgcggtat tatccatcag ggagcgcttt
     2521 ctggtttgtt ttacggccaa gctcacccag cgggcaaacc ccttccgatg cacgacatat
     2581 tcgtagaaaa gcgacaaaca ttttacgaag caagcaattc tatcggcaat tttgtcgcct
     2641 tcaccggaaa catttcctat tgccattccg cactcctcgg atgcatgccg cttggcagca
     2701 cccggcgacg ggaccagagg aagccatgcg gcgcgaaggc cttgaagtga tctggagagc
     2761 aggatgacca tgacgctgga cgagatggcc gcgcggcttg ccgcgttgga ggacaaggaa
     2821 gcaatccgca ccttgaaggc gcgctacttg cgggcctgtg acctcaagca gccggatgcg
     2881 gtgcgggatt gctttgcgcc gggcgaaatc cgcatcgcct accagaactt ccccgagttc
     2941 acggaccgcg aggccttcgt cgagacctat cgtgccatgg gctgccagcc cggcgtctat
     3001 gacatgcatc acgccaccaa ctgggaaatc gacctgcttg ggccagaccg cgcggcgggc
     3061 aaatggtcgc tcaacttccg caccatcctg gccggaccac gccaggtcat ccggctcgtg
     3121 gtcgaatacg acgacgttta cgaaaggcgc gatggccgct ggtggatcgt ggagacggtc
     3181 agccgggtca cctcgatgct gatggagcag gtgggcgagg acggcaacat cacggtcggg
     3241 gtgctggcgg aaccgccggt cgcctgattg ccggcgggga gaggcctgcc gaccagaaca
     3301 ataccaatga gtggagggat acggacatga ttgactacgg cctgggcgga aaggtcgcgc
     3361 tcgtcactgg tgcgggcggc ggtatcggac gggcggcggc cctcgtcttt gcgcgatcgg
     3421 gcgccagcgt gctggtgagc gacatcaacc aggcaggcgg cgaggaaacc gtggggatga
     3481 tcgaaagcgc cggtggcaag gccctgtttc agcgctgcga cgtcgcccgg tccgaggagg
     3541 tcaaggccat ggtcgccgct gtcgtggcgg cattcggccg gctggactgc gcgttcaaca
     3601 atgccgggat caaccaggtg ggcgacgacg agtacgacga cgccatctgg gagcgcaata
     3661 tcggcatcaa cctgtcaggc gtgatgcgct gcatgcgcga ggaagccgag gtcatgctcg
     3721 cgcagggcgg cggggccatc gtcaatacgg cctcgatcaa cgggctggtt ggtaaccctg
     3781 cgcagcccgc ctatgttgcg gccaagcacg gggttgtcgg cctggcgcgc cacggtgcgc
     3841 tgcgctgggc ccaggccggc atccgcgtga acgccgtctg ccccgggatc atcgaaacgc
     3901 cgatgacggc gccgctggtc accgatccgc agatgaagca actgatggac tcgatgaccc
     3961 cgatgggccg catgggcaag gctcaggagg tggccgaggc cgtggtctgg ctgtgttcgc
     4021 cggcggcgag cttcgtcacc ggccatgcca tggtggtcga cgggggagcg accgcggtct
     4081 gatccgcccc gccggtctag taaaccgccg cggggttctt cggacgcggg ctgccgagca
     4141 tatcgcgatg ggaaggacgg tcctggccgc gatcggtcac gcccagctcc cgggcgattt
     4201 ccgccacgat cagggtctgg ccggacaact cgtcgcggtt cggcgcgcgg tcgatggcat
     4261 cgatcacgtg ggccgagaac tcggggtttt cggccatggc gaggaaggcc tcgtattgtt
     4321 cggggtgcgt cctggccgag atctgcgatc gttcggtcac ctgcgggccg agccacaagg
     4381 acacggcggc gacaccggtg ccgcggaaat catgctccat gtcgtgcgcc agcttgtcga
     4441 gccccgcctt ctgggcgcca taggcagggc cgtgcatgta gcaggttgcc ccgaaggacg
     4501 aggtcatcgc gatgatcccc gagccctgcg gaaccatgat ccgcgccgcg tgccagctgg
     4561 ccacgtaggc actgcgcagg ccgacatcga ggatcttctg ggcgtcgagg tctttctccc
     4621 agaacggcct cttctcgatc agctggtcgt gaatataggc ggcgttgttg acgagcacgt
     4681 cgagcctgcc ctgctccgtc ctgatctgat cgaacagccg cgcgaccgaa gcgtcgtcgg
     4741 catggtcgca gaccaccggg atgcccttgc ccccgttttc cgtcacgcgt tgcgcggtct
     4801 ctgccacggt gccgggaaga accgagccgt cccagccctt cgtatcgccc ggcgtgaccg
     4861 tgcggcccgt gacatagacg gtgtagccca tttcgccgaa gccgcgggcg atccccgcgc
     4921 cggctccccg gctggcgccg gtgacgagcg cgaccttctg ctgggtcatc gaatgtcctc
     4981 tctcaatggt gcgtgtgggt gcccgacgac tggcggaagg ccagcggggt ctggccggtg
     5041 gtgcgccgga atgcccgggt gaaactgttg gcatgggaaa agccgcaagc ctgggccacg
     5101 gccttgatcg gctgggcgct gccttgcagc atttccttgg cctgctggat gcgggcggcc
     5161 tcgatgtagc cagacacgct cctgccggtc agtgcatgaa actggcggtg caggtgacgc
     5221 aggctgatgc cgcacagcgc cgccagctcc gccgcgcaag ggaccgggcc cggctgggca
     5281 atgcgctcgc ggatacggcg gaactgccat ggcgccagcc ggcccgagat cgacgcagaa
     5341 gcctcgcggg tgatcacgcg ttcggcctcg accgccacga gctctaccaa tgccgcaacg
     5401 gccgcatcgc tgtgaccatc cgggttctcc agctcccgcc gaagaaggtt catgatggcc
     5461 cgcaaggtct cattgccgat ggcgagcatg gcctgcagga aatcaagatc ggggtcggct
     5521 cgcaaggcgg tgagggcgcg ggtgcgttcc gggtcgaaca cgcagcgcac gacgcggatg
     5581 tgcccggctt ccgagcgccc ggagacgagc acgccgggcc agcggatgaa cagggtgccc
     5641 atgaaacagc ggcgtaccgc gtcgagctcg ggaaagcagg cggaggcgtc ggccgaggtt
     5701 ggcggcagcg acatctccac catcagcgcc tcttcgcgca tggtgaaatc gaggatggcc
     5761 ggccagcgcc actcaacgat ctccgcccgg ccgaggggcg tgtgggcctg gacaatcgcg
     5821 gtgggggtgt agtcggtcat ggccctgcgg ctcaggcgaa gtcgaactgg gacgtgggga
     5881 tgatgtcgat ccggctgaaa tcgatgaagc cggtgcagct ttccagcatg gccgcggtgt
     5941 tcgcggcgaa ccgctccggc tggtcgacgg catcgaagaa cacttcaggt accgtcagcg
     6001 cctcaagcgg gaagcactcc tcgacgaagg cgtcgtaggc cggggcacca tcggtcagag
     6061 ggcggacgat gatgttctgg acatattcga aattggactg cgtttcgatc gccacgcggg
     6121 tgtgatgcga atgccagtgg gcgatcgcct cctgccgcga caggtccggg cggaacgaga
     6181 tgaagctcgc ctggctccag ccccatgtgc gcgacggcgc ggccttgccg gaggcttgct
     6241 cggggccgat gctgttcggg gtgaccgggt agccggtgtt ggcgatgatc gtggattcgc
     6301 acaccagcca ggcagcaaac gaggccgaat gggcggcgag cgcgctgtcc accgcgccgc
     6361 ggaagcgggc attggccgag ggcatccaga actgggcgac agcggcctgc tggggcgcct
     6421 gccagcgctg gatcagccct tcgcctgccg ccacggcggc gtcccgaacg ttcaggcgga
     6481 tcttctgcgc gccggcggcg gcgagcgcgg cgggcagggc ggaaaggatc cgggcgccat
     6541 aggcggcgtg gtcctctccc tcgggagccc acagggcggc gatcaccttt tccatcgggc
     6601 gctgctccta gtcgaagttg gcctgcccgc cgtcgagcgg aatggttgcg ccggtgagat
     6661 aggcaaggtc ctgcccgcac agcgccacca gcgcccggcc gatgtcctgt tccagttcgc
     6721 ccaccctccc gagcgggatc gtgcggaaga aggcctcggc ctcttcaggg ttgttctcga
     6781 tccaccacat cagccccggc gaggcggcgt gcggcgctat ggtgaggacg cggatacctt
     6841 cgctgcccca ttcggccgcc gccgcgcggg taagggcgcg gattgcctgc ttgaccgcgc
     6901 cataggcgcc atagccgctc atgtcccagc ggattccggc cgatgagcag aagttgaaga
     6961 tcgtcccgcc gccgcgcgcc gccatgtggg ggcgggccag cttcatcagg cgcatcgccg
     7021 cgagaggccc cgatacgaag cccgcggtaa aggcctcgtc cgtaacatca tcgagcttgc
     7081 cgagcggcac ttcctgggca ttgttgacca ggatgtcgag cccgccgaac tggttgaccg
     7141 cagcctcgac agcggcggcc aggtcgtcgg ccgatttcac gtcgcaggcg agcggcaggg
     7201 ccgttccgcc ccgttcggcc acaagctgtg cggtctgttc caccttcgag agggtacggc
     7261 cggtcaccac gaccgtcgcg ccagcagagg ccatggccag ggccaccccc tggccaattc
     7321 cttgtcccgc gcccgtgatc agcgccacct tgcctgagag atccgtcatc gtgcatccat
     7381 ctcgttcatt gcgttcgttc agaagatttc ccggccttcc ccgaagacca ggctggctgc
     7441 ccgattgtcg cgcgggccgg tgaagccttt cacgcccagt tcggcccgca cctgcgacgg
     7501 cgtcaggtgc atgagctcct cccagcgcac gatgaacagg ttgggcgact tgctgccgac
     7561 ttcgtagcca tgcgtgaaat tgtcccacac caccggccag atctgcggat aatgcagcat
     7621 ggtgcgcatg gcccagggga aggtcacgaa catcgtcgtg gtcatcaatt cgcctgccag
     7681 ctccggcgaa acatgggcga acaggttggc cgtgcgcagg ccggcgggga atacctcgcc
     7741 gatcgcgtcg aagccgcact cgccgatcag gtgatcgaaa tcgtgggtct gcccgctgcg
     7801 cagcgtgtag taatcgagat cgctggcggg cgcccacgac ggatcggcgt gccgctgggg
     7861 caggagttcg agcgtgaggc cgagcgcgac catatggtca tggagcaggc gaccgatcga
     7921 gcccgcaggg ttcgccgcga gatcgtcaat cgtgtaggtc gagatgaagc gctcgtcgaa
     7981 ccagcggtcc agggccttgt tcgtacggcg ctccgccttg atcagcgcct cgatgcgctc
     8041 ccggttacgg agctggtcga ccgtctcgac catgtccggc aggccctcga tcaagctttg
     8101 cggtccgttg ttgcggcgca aggtgtgaaa cgccacccaa tcgcggacgc gcgcgtcgtc
     8161 aagccagggg gaagatccgg cggcgctgtc gcaaaggggc atcgtgaccg ccattgctgt
     8221 cactccttgg tgatgtagcg ggcacggtag gaggcgaatg cctcgcgcac gtcggcttcg
     8281 ttgagcccga agcgctcgag cgaatagtcg tgcagcgggc gctgctcgcg cttgttgccg
     8341 gccatgaact cggtcagcgc ggcctcgatc gcggcatcca gcgggatgcc gatccgggcg
     8401 agcacgcgct ccgcctgctt gagcggttca cgcgcaacct cgcgatagtc gatatcgatg
     8461 aagcggtcct cgccgatccg cgcccgcgag gcttcgaacc gcttcatcca ctcggccagc
     8521 cggggaaacc agaagccgcc gacggcctcg tccgaatgga ccgagttcag cttgtagagt
     8581 gccgcttcca tgctgacata gcttggtacc acctcgagcg gatcgcggtg cgtcatcacc
     8641 agtaccgcct gcgggaacgc cttggcgatg atgtccgtgt agggaaggtt ctgcgggctc
     8701 ttcaggatcc acttcttgcc gcgccgggtg ggatcctgcc actgcaggta cttgaggatc
     8761 gtcttgaggt cctcgtaacc ttgcgactgg tcatagccat tgagccattc cgcgaaggac
     8821 gggaggaacg tcatcgcctc caccatggtg gtgatgaaca gctgcccgag gatgatgatc
     8881 tcctcgtcgg gagcgtcagg atccagcggg tggatcgagg cgagctcggg cgagaggttg
     8941 agccagcccg agatcatcgc ctcggcatag gcgcgccggg cctgcgggtt gccgggctcg
     9001 tctcccggca ggggagcata gttctgggcc tcgtaccagc ggatggcg
//
LOCUS       DATNBV010000498         5712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2840178,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000498 DATNBV010000000
VERSION     DATNBV010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2840178"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /gene="wecC"
                     /locus_tag="VN627_11800"
     CDS             complement(<1..1113)
                     /gene="wecC"
                     /locus_tag="VN627_11800"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HXD03979.1"
                     /translation="MKSDKPEVCVVGLGYIGLPTAAIVARAGCRVHGVDVSPRVVETI
                     NRGEIHIEEVDLDGLVQGVVARGLLRASTRIEPADVFVIAVPTPFEDGHAPDLSFVLE
                     AGRAVATVLKPGDTVILESTSPVGTTDRLRDLVAELRPDLRLPGLSGETPDVAFAYCP
                     ERVLPGRILEELTNNDRSIGGVTPRCARKALAFYKLFVRGACVTTDARSAEMTKLVEN
                     AYRDVNIAFANELSIIADTMDLDVWEVIRLANRHPRVNILSPGPGVGGHCIAVDPWFI
                     VHSAPEQTPLIRTARGVNDAKIHHVIERADALLAANPEARVACLGLAFKANIDDFRES
                     PARLVASTLTRKYADRISVVEPFITELPIEYRDTPVE"
     gene            complement(1094..2233)
                     /gene="wecB"
                     /locus_tag="VN627_11805"
     CDS             complement(1094..2233)
                     /gene="wecB"
                     /locus_tag="VN627_11805"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HXD03980.1"
                     /translation="MTAAPTRILVVFGTRPEAIKLFPVVAALKADPRFEPVVCVSAQH
                     REMLDQVLAIAGLVPDHDLDLMQPGQTLDGLTAALLTSLGKVMDEVRPDRVMVQGDTA
                     TAMAGALAAYYRKLPVDHVEAGLRSGNIHHPWPEEVNRKIIGAMASLHFAPTEVSAAA
                     LHRENVDPARVHVTGNTVIDALHWVTQRIAANPALACGLAGVEERIAGKRIIGVTTHR
                     RENFGEGMDAIANAIRRIAARPDVAVVFPVHLNPNVRAVMNERLAGLDNVALLEPLDY
                     PHFARLLALAEIMLTDSGGVQEEAPALGKPVLVMRETTERPEGVSAGTARLVGTDEDR
                     IVTEIFTLLDDKAAYEAMARAHNPFGDGRSAARIVELLGNEIGQA"
     gene            complement(2312..2893)
                     /locus_tag="VN627_11810"
     CDS             complement(2312..2893)
                     /locus_tag="VN627_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03981.1"
                     /translation="MIRPLALAAAAPLALLAFTTPAAAQDLTDPSGERVNALIVYGND
                     PCPVGKGDEITVCARKPESERYRIPAPLREQPTTRSEAWNEKVLAYETVGRTGTQSCS
                     PVGPGGSTGCTQKLIDRAFAEKRQSTDVGFAKMVDEERARRAGTVDASAAATQARVEQ
                     AEKDYEARERAREEAAAKPAPGAGDPTVPATKP"
     gene            complement(3009..3533)
                     /locus_tag="VN627_11815"
     CDS             complement(3009..3533)
                     /locus_tag="VN627_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HXD03982.1"
                     /translation="MSKHTARNIGRILRVDQAGEFGAIRIYAGQLAVMGDRAPHAREV
                     AAMAAQEEDHRRRFDAMLAQRGVRPTALAPVWSVAGYALGAATALMGPEAAMACTAAI
                     ETEIDRHYTQQLEELGDSDPELAATITEFRDHEREHKQAALEAGAERAPAYPLLSGAI
                     RLGCRLAIRLSERI"
     gene            complement(3537..4019)
                     /locus_tag="VN627_11820"
     CDS             complement(3537..4019)
                     /locus_tag="VN627_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HXD03983.1"
                     /translation="MLPKGEAALGLARVVALVVPAGLLAGAYVSQYGFGLYPCEMCWW
                     QRYAHFAALPFALLAFALPARRWPLALATIAIAASALIGAYHAGVEYHWWQGLTACTS
                     TADLSSGDALDAILNAPMIRCDQAQWALFGISLAGYNFLISGAGAVLAALLLSRSRKA
                     "
     gene            complement(4034..5389)
                     /locus_tag="VN627_11825"
     CDS             complement(4034..5389)
                     /locus_tag="VN627_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXD03984.1"
                     /translation="MSSAIRKFRPLLRAALLVTAVAMIPATTAGLAAVDASAGPEFGR
                     LFTVYQIIKANYVEPVDDDKLIKGAIQGMLTSLDPHSSYLDGASLSRLDTLIQGNYSG
                     LGLSVVGDDGAVKVVSPMRGSPADKAGVKAGDYITHLDGKLIYGLDLDEAVAKMRGPA
                     GTSIRLTIFRPGRDEPFDVTVTRGVISLEPVTSKLTDNVGVITVNEFSADVGNYVNRE
                     LAKLKQQSGGKLAGVVLDLRQNPGGALDEAVALSDLFLTSGDIVSQRGRVASENEYYR
                     AETVFKGDAAKGLPLVVLINSGSASASEIVAGALQDHHRALIMGERSFGKGSVQSMIR
                     LDKTHAVKLTTARYYTPSGRSVQEGGIEPDIRVPQISDPDARKRAALSYRESDLRGHL
                     VNDVDLDDKKLEDDKQQDPRFKMTPEELKAKGIEDFQLWYAINTIRRTIVPAPAARAA
                     K"
BASE COUNT          918 a         1766 c         2110 g          918 t
ORIGIN      
        1 ttccaccggc gtatcgcggt actcgatggg cagctcggtg atgaagggtt cgaccacgct
       61 gatccggtcg gcgtacttgc gcgtcagggt actggccacc agccgggccg gactttcgcg
      121 gaaatcgtcg atgttggcct tgaaggcgag gccgaggcag gcgacgcggg cttccgggtt
      181 ggcggcgagc agggcgtcgg cgcgttcgat gacgtggtgg atcttggcat cgttcacgcc
      241 gcgcgcggtg cggatcagcg gggtctgctc cggggcggag tggacgatga accacggatc
      301 gaccgcgatg cagtggccgc cgacgccggg gccgggggag aggatgttga cgcgcggatg
      361 ccggttggcg aggcggatca cctcccacac gtcgaggtcc atcgtgtcgg cgatgatcga
      421 cagctcgttg gcaaaggcaa tgttgacgtc gcggtaggcg ttctcgacca gcttggtcat
      481 ctcggccgag cgcgcgtcgg tggtgacgca ggcaccgcgg acgaagagct tgtagaaggc
      541 cagcgccttg cgggcgcagc gcggggtgac gccgccgatc gagcggtcgt tgttggtgag
      601 ttcctcgagg atgcggccgg gcaggacgcg ctcggggcaa taggcgaagg caacgtcggg
      661 cgtttcgccg ctaaggccgg gcaggcgcaa gtcggggcgc agttcggcga cgagatcgcg
      721 caatcggtcg gtggtgccga cgggcgaggt cgattcgagg atcaccgtat cgccgggctt
      781 gagcaccgtg gcgacggcgc ggccggcttc gaggacgaag gacagatcgg gggcgtggcc
      841 atcctcgaac ggggtgggca cggcgatgac gaagacgtcc gccggctcga tccgggtcga
      901 agcccgcaga agcccgcggg cgacgacacc ctggaccagg ccatcgagat cgacctcctc
      961 gatatgaatt tcgccgcggt tgatcgtctc caccacgcgc ggcgacacat caacgccatg
     1021 cacccggcat ccggcgcggg cgacgatggc ggcggtgggg agcccgatgt accccagacc
     1081 tacgacgcag acttcaggct tgtccgattt cattacccag cagctccacg atccgggcgg
     1141 cgctgcggcc atccccgaag ggattgtgcg cgcgggccat tgcctcatag gccgccttat
     1201 cgtcgagcag ggtgaaaatt tcggtaacga tgcggtcctc gtcggtgccg acgaggcgcg
     1261 cggtgcccgc ggagacgcct tcggggcgct ccgtcgtttc ccgcatgacg aggacgggct
     1321 tgccgagggc cggcgcttct tcctgcacgc cgcccgaatc ggtgagcatg atttcggcga
     1381 gcgcgagcag gcgggcgaag tgcggatagt cgagcggttc gagcagggcg acattgtcga
     1441 gcccggccag ccgctcgttc atcaccgcgc ggacattggg gttgaggtgg accgggaaca
     1501 cgaccgcgac atccggccgc gcggcgatgc ggcggatggc gttggcaatg gcgtccatcc
     1561 cctcgccgaa gttctcgcgg cggtgggtgg tgacgccgat gatgcgtttc ccggcgatgc
     1621 gttcttcgac cccggcgagg ccacaggcga gggcgggatt ggcggcgatg cgctgcgtga
     1681 cccagtgcag cgcatcgatc accgtgttgc cggtgacatg gacgcgggcg ggatcgacat
     1741 tctcgcggtg gagcgcggcg gcggagacct cggtcggcgc gaagtgcagg ctggccatgg
     1801 cgccgatgat tttgcggttc acttcctcgg gccagggatg gtgaatgttg cccgagcgca
     1861 ggccggcctc gacgtgatca accgggagct tgcggtaata ggcggcgagg gccccggcca
     1921 tggcggtggc ggtatcgccc tggaccatga cccggtcggg ccggacctcg tccatgacct
     1981 tgccgaggct ggtgaggagg gcggcggtga gcccgtcgag cgtttgcccc ggctgcatca
     2041 ggtcaagatc gtgatcgggg acgaggcccg cgatggcgag gacctgatcg agcatttcgc
     2101 gatgctgggc cgagacgcag accaccggct cgaatcgggg atcggccttg agcgctgcga
     2161 caaccgggaa cagcttgatt gcttccgggc gggtgccgaa gacgacgagg atgcgggtag
     2221 gggcagcggt catggtgggc cgcctagcat gggcgggtta agtttggcta agcgcggctg
     2281 actcccttcc gcttgcggga agggagaaag gctacggctt cgtggccgga accgtcggat
     2341 cgccggcgcc cggggcaggc ttggcggcgg cttcctcgcg ggcgcgttcg cgggcttcgt
     2401 agtccttttc ggcctgctcg acccgggcct gggtggccgc ggcactggca tcgaccgtgc
     2461 cggcgcggcg ggcgcgttcc tcgtcgacca tcttggcgaa gccgacgtcg gtcgactggc
     2521 gcttttcggc gaaggcgcgg tcaatcagct tctgggtgca gccggtggac ccgccggggc
     2581 cgaccggcga gcacgactgg gtgccggtgc ggccgacggt ttcgtaagcg agcaccttct
     2641 cgttccaggc ctccgagcgc gtggtgggct gttcgcgcag cggggccggg atgcggtagc
     2701 gttccgattc gggcttgcgg gcgcaaaccg tgatctcgtc gcccttgcca acggggcagg
     2761 ggtcattgcc atagacgata agcgcattga cccgctctcc gctcgggtcg gtcagatcct
     2821 gcgcggcggc aggggtggtg aaggcaagca gggccagcgg agcggcggcg gcgagggcaa
     2881 gcggtcggat catgagcggc tccggcagga tgtttcggca actatagggc gacttgccct
     2941 tgaacgatag atgaagcggc cgaacggatg ccgccttgcg ctggcgcaaa tcgcgcccgc
     3001 gcagcagctt agatccgctc ggacaggcgg atcgccagac ggcagccgag gcggatggcg
     3061 ccggaaagca ggggataggc cggcgcccgc tcggccccgg cctccagcgc ggcctgcttg
     3121 tgttcgcgtt cgtgatcgcg aaactcggtg atcgtggcgg cgagttcggg atcgctgtca
     3181 cccagctctt ccagttgctg ggtataatgc cggtcgatct ccgtctcgat cgcggcggta
     3241 caggccatcg ccgcttccgg gcccatcagc gcagtggcgg cgccgagggc atagccggca
     3301 accgaccaga ccggcgcgag cgccgtgggg cgcacgccgc gctgagccag catggcatcg
     3361 aagcggcggc ggtgatcctc ttcctgtgcg gccatggcgg cgacctcgcg ggcgtggggc
     3421 gcgcgatcgc ccatgacggc gagctggccg gcatagatgc ggatggcgcc gaattcgccg
     3481 gcctggtcga cccggaggat gcggccgatg ttcctggcgg tgtgcttcga catgggtcag
     3541 gccttgcgac tgcgcgagag gagcagggcg gcgagcacgg cgccggcccc tgagatcagg
     3601 aagttgtaac ccgccaaaga gatcccgaac agggcccact gggcctggtc gcagcggatc
     3661 atcggcgcat tgaggatggc atcgagggca tcgccggagg acaggtcggc ggtggaagtg
     3721 caggcggtga ggccctgcca ccagtgatac tcgaccccgg cgtgataggc cccgatcagc
     3781 gcggaggcgg caatcgcgat ggtggcgagg gccaagggcc agcgccgcgc gggcagggca
     3841 aaggcgagga gcgcgaaggg cagggcggcg aaatgggcat agcgctgcca ccagcacatc
     3901 tcgcaggggt atagcccgaa gccgtattgc gagacatagg ccccggccag cagccccgcc
     3961 ggcaccacga gcgcgaccac gcgggccaga cccagcgccg cttccccctt cggcaacatg
     4021 gctctcgtcc cccctacttg gccgccctcg cggcgggcgc cggcacgatg gtccgccgga
     4081 tcgtattgat cgcgtaccac agctggaaat cctcgatgcc cttggccttg agttcctccg
     4141 gcgtcatctt gaagcgcgga tcctgctgct tgtcatcctc gagcttcttg tcgtcgagat
     4201 cgacatcgtt gacgagatga ccgcgcagat cgctctcgcg ataggacagg gcagcgcgct
     4261 tgcgggcatc ggggtcggaa atctggggca cgcggatatc gggctcgatc ccgccttcct
     4321 gcaccgagcg gcccgacggc gtataatagc gcgcggtggt cagcttgacc gcatgggtct
     4381 tgtcgagccg gatcatcgac tggaccgaac ccttgccgaa cgaacgctca cccatgatca
     4441 gggcgcggtg atggtcctgc agggccccgg cgacgatctc cgaagcggaa gccgagcccg
     4501 agttgatcag gacgaccagc ggcaggccct tggcggcatc gcccttgaac acggtttcgg
     4561 cgcggtaata ctcgttttcg ctggcaacgc ggccgcgctg ggagacgatg tcgcccgagg
     4621 tgaggaacag gtcggacagg gcgacggctt catcgagcgc gccgcccgga ttctggcgca
     4681 ggtcgagcac gacgcccgcc agcttgccgc ccgactgctg cttgagcttg gccagctcac
     4741 ggttcacgta gttgcccaca tcagccgaga actcgttgac cgtgatcacg ccgacattgt
     4801 cggtgagctt ggaggtgacc ggctcgaggc tgatcacgcc gcgcgtcacc gtgacgtcaa
     4861 agggttcgtc gcggccgggg cggaagatag tcaggcggat cgaggtgcct gcgggaccgc
     4921 gcatctttgc gacggcttca tcgagatcga ggccatagat cagcttgccg tcaaggtggg
     4981 tgatatagtc gcccgccttt actccggcct tgtccgccgg actgccgcgc atcggcgaga
     5041 caaccttgac cgcgccatca tcgcccacga ccgagaggcc gaggcccgaa tagttgccct
     5101 ggatcagcgt atccagccgg ctcaggctcg ccccgtcgag ataggacgaa tgcggatcga
     5161 ggctggtcag catgccctgg atcgcgccct tgatcagctt gtcgtcgtcg accggctcga
     5221 catagttcgc cttgatgatc tggtagaccg tgaacaggcg gccgaattcc gggcccgccg
     5281 aggcgtcgac cgcggcaagg cccgcggtgg tggccgggat catcgcgacg gccgtgacca
     5341 gcagggcggc gcgcagcagg ggacggaact tgcggatcgc ggaagacatc ggccggaaag
     5401 cgcctttcgg ggcagggatt tggggccacc ctatagggcg gcgcggctga acgccagatg
     5461 gcggctatac ccttgctccg ttcctcccga gcgaagtcga gggactaggc cgagcgtcag
     5521 cgaggctgca gcggtgcggg ttctcgctag cgctcgaact agtccctcga caagctcggg
     5581 aggaacggag agttggggag ttccctaaaa acgcgcgaaa tccagcgggt tcaccggctc
     5641 cccgtcgcgc cgcagctcaa ttgaaaccac cggccgcccg ggcccggccg tgccgagcgg
     5701 cgagccggcg ag
//
LOCUS       DATNBV010000499         8079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3776598,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000499 DATNBV010000000
VERSION     DATNBV010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3776598"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VN627_11830"
     CDS             <1..727
                     /locus_tag="VN627_11830"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXD03985.1"
                     /translation="WARRAEVTTPYAAKGVQVAASAAELGAACRHVGICVRNDAGVLE
                     VCDALIPAMAPGALIAVHSTILPATSEQLAARCASVGLQFVDAPVSGGALAAQAGTLT
                     VMCGASDEAFATARPVFETFGRLIVLLGPAGSGQRAKLVNNALLSANVALAWAALQAG
                     QALGMDRAALAAVIAQSSGASTGLTHAAAIADPTEYSLGGPLLRKDLDLLCAAIPDDP
                     GAMALDRAARSFLLAATGEGEAP"
     gene            865..1650
                     /locus_tag="VN627_11835"
     CDS             865..1650
                     /locus_tag="VN627_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HXD03986.1"
                     /translation="MTGEQRIEAPIEVDWAALNDPDVLRACIPGCQSLEVIGENRFRA
                     SATIKVGPISARFGGEVQLSELDPPNGYRITGEGSGGAAGSARGGARVALTRDGAATL
                     LAYEVDADVQGRLASLGGKLIDVTAKQLAGVFFSRFAQEVGSRDFSEAAVAPAAAAAP
                     AASASAAPRQPVAPAPAAPASSLGWLLALAAALVGGFILGRAASGWDGSGSLAGMAVG
                     LLTVLAAFAGFAFGRRQPAQPQLVVLDADMLATLVARSREKGA"
     gene            1647..2513
                     /locus_tag="VN627_11840"
     CDS             1647..2513
                     /locus_tag="VN627_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HXD03987.1"
                     /translation="MKAAPFDYVEAGSLDEAVHLMGEAGSDGAVLAGGQTLMPLLALR
                     VASPSVLVDINLAADMAGITETGGALRVAARTRQAHARKDPLVARHLPLMAEALGHVG
                     HFQTRTRGTIGGSVALGEPAAEMPALAVALGANVELRSVRGTRMVPAREFYVGPYSTV
                     RADDEVLAAVHYPAWPQNTRTLFEEVARRQGDFALVGLLGGMVVEGGRIARAGLAWFG
                     MGPTPVPAVAVERALVGTAPADVDVADLTRIALGEVAPGDDLHASEQYRRTVAARVFA
                     RRMRTLLAELAA"
     gene            2510..2992
                     /locus_tag="VN627_11845"
     CDS             2510..2992
                     /locus_tag="VN627_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXD03988.1"
                     /translation="MSLHFIEFELNGRTAEGMAESRRTLLDYLRQDEGCTGPHAGCEH
                     GVCGACTVLVDGEPMRSCLMLAVQANGKKVETLEGLAAPDGALHPVQQMFREEFALQC
                     GFCTPGFVMTMVALDRAEPRPDAAEMKQALSGHLCRCTGYQGILRVVDRLAAGEGEAK
                     "
     gene            2989..5310
                     /locus_tag="VN627_11850"
     CDS             2989..5310
                     /locus_tag="VN627_11850"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXD03989.1"
                     /translation="MNDPAPVAGARFVGQRVQRKEDRRLLTGKGTYVDDVVVPGMLHA
                     AFLRSTVARGRITRLDVTAARALPGVVAVYTAADIDTLRPRFFNSAMDQPDLPYPDNG
                     LVARKDVRFVGDPVAIVVAESRALAEDGAALIEVDYAVEPPIVGIAAARTMPPVHPEY
                     ETNAIGEMEMAIGDDVDAIFASAAHVVEDTIVNCRQTQLPMETRGIVARREGAGELTI
                     NLACQGTHMAATHIANVFGLPHNEVRVLAKDVGGGFGQKVTALRDELAIVGAALLLNR
                     PIKWIEDRLESLTACSQGRDEHCRVKLAFDADHRLLAADVEFHVDYGAYPHAAHGSGG
                     LVAMTFPGPYRLPAYRFKSSGWFTNTCGEGPYRGPWMMDMFARETMLDKAARQMGVDP
                     VELRRKNIIGRPDQPFTMVTGLVLDDVTPRETMDATVAAIDVPAFRREQEEARKQGRY
                     LGLGVAATIEPTAIAFGTYSSEVAHVRVEPTGKVTAMLSTLSQGHGTATTMAQIVADR
                     LGVPFADVTIVEGDSQRTGYGSGAGGSRQAVVGGGAATVAADRLAEKIKVITAHAFNA
                     SPEAVQIDNGQITIQGSEMRASLAEIAAMAYFDTDRLPHGMESGLETQYRFRTPPMVF
                     ANAAHACIVEVDIETGKVKILRWVAGGDCGNLINPAIVEGQISGGVVQGIAGVLFEKV
                     AYDANGQPLAATLKDYLVPTALDVPRLEYVHLCTPSTTPGGYKGVGEGGAMIAPPTLV
                     NAINDALAPFGKTWLTMPLSPDRIVCGLADVEA"
     gene            complement(5382..6110)
                     /locus_tag="VN627_11855"
     CDS             complement(5382..6110)
                     /locus_tag="VN627_11855"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXD03990.1"
                     /translation="MSSPVFSLVNALMPRLSSANEFGVMFSDQERAAVLRGLFESYYA
                     GRPEQVVFDTNRSWTQKLPVLLELFPATKLVCLVRPIGEILQSFEHQFLRHPLQLSQI
                     IGCDPDTSVYTRTDRLMFANGLVGVALNGLKEAFYGAASERLMLVSYQSLVTAPAEVI
                     SAIYRFCGLQSFAHDFNNVTFEEQAFDHKLGAPGLHQVRQAVARIPTRPTLPPDILAR
                     HAGAHFWETDASLTQADCNWAAMR"
     gene            complement(6204..6920)
                     /locus_tag="VN627_11860"
     CDS             complement(6204..6920)
                     /locus_tag="VN627_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03991.1"
                     /translation="MNYVTSIFRFGMAANRFGLQTTLHMHSESLVTRGRNEMVRFFLE
                     HEEFTHLLWVDSDIEFQPRDAFRLLLSDLDVVAGIYPIKNLNWPAEGISEAMTLDEYV
                     VRHATFPFNPVGMWEEPIVPRLNADEFIEVAEAPTGFMCIKRDVIYRMMEHYPELNYM
                     PEGAEKQAKKQFYWLFFDCMVEPETKRYLSEDYAFCKRWRDMGGKVHADFQSDLGHLG
                     QYMYRGNLSRSIRRRDGLPY"
     gene            complement(7261..>8079)
                     /locus_tag="VN627_11865"
     CDS             complement(7261..>8079)
                     /locus_tag="VN627_11865"
                     /inference="COORDINATES: protein motif:HMM:NF015831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HXD03992.1"
                     /translation="AVGANASAAGTQAFASGTSASAFGDRAQALGDSATATGVLAVAN
                     GAAATAGGAGAFANGAQAIAFGNNATANGATASAFGGTANAAANQSTAIGFAATAVAT
                     GSTAVGYNARANNPNDVALGSNSVTAPSNPTSGASVDGWGFTNFPGNNPQSVVSVGSQ
                     GAERQITNVAAGRITPTSTDAVNGGQVYWITDTLSRKLSAGIASAMSMAAMPMPTEPG
                     AWMFSGGFGYWHGQTGYSLGLAKTTDDGKWSFRAGGTVDSQGTTGASAGFGIQF"
BASE COUNT         1299 a         2746 c         2676 g         1358 t
ORIGIN      
        1 ctgggcccgc cgcgcggaag tcaccacgcc ctatgcggcc aagggggtgc aggttgccgc
       61 ctcggcagcc gagcttggcg ccgcctgccg ccatgtcggc atctgcgtgc gcaacgatgc
      121 gggcgtgctg gaagtctgcg atgcgctgat cccggccatg gcgccaggcg ccctgatcgc
      181 ggttcattcg accatcctgc cggcgaccag cgaacagctc gcggcccgct gtgcaagcgt
      241 gggtctccaa ttcgtcgacg cgccagtgag tggcggagca ctcgcagcac aggccggcac
      301 gctgaccgtc atgtgcggag ccagcgatga agcctttgcc acggcccggc cggtgttcga
      361 aaccttcggc cgcctgatcg tcctgctcgg gccggcgggg tcgggccaac gggccaagct
      421 ggtcaacaat gccctgcttt ccgccaacgt cgccctcgct tgggccgcgc tgcaggccgg
      481 ccaagcactg gggatggacc gggcggcact tgctgccgtc atcgcgcaga gcagcggtgc
      541 cagcaccggg ctcacccatg ccgcggccat cgctgatccc accgagtaca gcctgggcgg
      601 tccgctcctg cgcaaggatc tcgacctgct ctgtgccgcc atccccgatg atcccggcgc
      661 catggcgctg gaccgggcgg cgcggagttt cctgctagcc gccacggggg agggcgaggc
      721 accgtgaagc gtcgagcatg cttgtgcttt cgcccgaaag gcggtttcgt tgcgcgcacg
      781 gggcaggcat gaagcgcacg aacaactgtt tcacggtccg tcagtgcgcc gggccggttt
      841 gaggagagta ttccggcaat gcagatgacg ggtgaacaac ggatcgaggc gccgatcgag
      901 gtggactggg cggcgctcaa cgatcccgat gtgctgcggg cctgcattcc gggctgccag
      961 tcgctcgaag tgatcggtga aaaccggttc cgcgcctcgg ccacgatcaa ggtcggcccg
     1021 atctcggccc gcttcggcgg cgaggtccag ctttccgaac tcgatccgcc caacggctat
     1081 cgcatcaccg gcgagggcag cggcggtgcg gctggctcgg cccggggcgg cgcgcgggtc
     1141 gcgcttaccc gcgacggcgc cgcgacgctg cttgcctatg aggtcgatgc cgacgtgcag
     1201 gggcgcctgg cctcgctcgg cggcaagctg atcgacgtga ccgccaagca gctcgccggc
     1261 gtattcttca gccggttcgc ccaggaagtg ggcagtcgcg acttttcgga ggccgccgtt
     1321 gctccggctg ccgctgccgc tcctgctgca agcgcttccg ccgcaccgcg ccagcccgtg
     1381 gcgccggcgc ccgctgctcc cgcatcgtcg ctcgggtggc tgctggcact cgccgccgcg
     1441 ctcgtcggtg gtttcatcct cggccgcgcc gccagcggat gggatggcag cggcagcctt
     1501 gccggcatgg ccgttggcct gctcactgtc ctcgctgcct tcgccggttt cgccttcggg
     1561 cgccggcagc ctgcccagcc ccaactggtg gtgctggatg ccgatatgct ggcaacgctg
     1621 gttgcccggt ccagggagaa gggcgcatga aggccgcacc gtttgactat gtcgaggccg
     1681 gctcgcttga cgaggcggtc cacctgatgg gcgaggctgg cagcgatggc gcggtcctcg
     1741 ccggcgggca gaccctgatg ccgctgctcg ccctgcgcgt cgccagcccg tcggtgctgg
     1801 tcgatatcaa tctggccgcc gacatggcgg gtattaccga gaccggcggc gccctgcgcg
     1861 ttgccgcccg cacccgccag gcccatgcgc gcaaggatcc gctcgtggcc cgtcatctgc
     1921 cgctgatggc cgaggcgctc ggccacgtcg gccatttcca gacccgcacc cgcggcacga
     1981 tcggcggatc ggtagcgctc ggcgaaccgg cggcggaaat gccggctctg gccgtcgcgc
     2041 tcggcgccaa cgtggaactg cgctcggtgc gcggcacccg catggtgccc gcccgcgaat
     2101 tctacgtcgg cccctactcc acggtgcgcg ccgatgatga ggtcctcgcc gccgtgcact
     2161 atcccgcctg gccgcagaac acacgcaccc tgttcgagga agtggctcgc cgccagggtg
     2221 atttcgccct cgtcggtctt ctcggcggca tggtcgtcga aggcgggcgg attgcccggg
     2281 ccggccttgc ctggttcggc atgggcccaa cccccgttcc cgccgtggcc gtggagcgcg
     2341 cgctggtcgg tacggcaccc gccgatgtcg acgtggccga tctcacccgg atcgcgcttg
     2401 gcgaggttgc tcccggcgac gatcttcacg catccgaaca ataccggcgc accgtcgccg
     2461 cgcgggtctt cgcgcgccgg atgcgcactc ttcttgcgga gttggcggca tgagcctgca
     2521 cttcatcgaa ttcgaactga atggccgcac tgccgagggc atggccgaaa gccgtcgcac
     2581 cctgctggat tatctgcgcc aggacgaagg ctgcaccggg cctcacgctg gctgcgagca
     2641 tggcgtttgc ggcgcctgca ccgtgctggt cgatggcgaa ccgatgcgct cgtgcctgat
     2701 gctggcggtc caggccaatg gcaagaaagt cgaaacgctc gagggcctcg ccgccccgga
     2761 cggcgcgctg caccccgtcc agcagatgtt ccgcgaggaa ttcgcgctcc agtgcgggtt
     2821 ctgcacccca ggcttcgtca tgaccatggt cgcgctcgat cgggccgagc cgcggccgga
     2881 tgcggcggaa atgaagcagg ccctctccgg tcacctgtgt cgctgcaccg gctaccaggg
     2941 catattgcgc gtggtcgacc ggcttgccgc cggggaagga gaggccaagt gaacgatccc
     3001 gctcccgtcg ccggcgcccg tttcgtcggc cagcgcgtcc agcgcaagga agatcgccgc
     3061 ctgctcaccg gcaaaggcac ctatgtcgat gatgtggtgg tccccggcat gctccacgcc
     3121 gcattcctgc gcagcacggt ggcgcggggc aggatcaccc gcctcgatgt caccgctgcc
     3181 cgggcgctgc ccggcgtggt cgcggtctac accgccgccg atatcgacac cttgcgcccg
     3241 cgcttcttca actcggcgat ggaccagccc gatctgccct atcccgataa cggcctggtc
     3301 gcccgcaagg acgtccggtt cgtcggcgat ccggttgcca tcgtcgtggc ggaaagccgc
     3361 gccctcgccg aagacggcgc tgccctgatc gaggtggact acgcggtcga gccccccatt
     3421 gtcggcatcg ccgcggcgcg caccatgccg cccgtgcatc ctgaatacga gaccaacgcc
     3481 atcggcgaga tggaaatggc gatcggggac gatgtcgatg ccatcttcgc ctcggcggcg
     3541 cacgtggtgg aggatacgat cgtcaactgc cggcagaccc agctgccgat ggagacccgc
     3601 ggcatcgtcg cccgccgcga aggcgcgggc gagctgacca tcaaccttgc ctgccagggc
     3661 acccacatgg cggcgacgca catcgccaat gtcttcggcc tgccgcacaa cgaagtgcgt
     3721 gtccttgcca aggacgtcgg tggcgggttc ggccagaagg tcaccgccct gcgcgatgag
     3781 ctggcgatcg tcggcgcggc gctcctgctg aaccgcccga tcaagtggat cgaggatcgc
     3841 ctcgagagcc tcaccgcctg ctcccagggc cgcgacgaac attgccgcgt caagctggcc
     3901 ttcgatgccg atcaccggtt gctggccgcc gacgtcgagt ttcatgtcga ctatggggcc
     3961 tatcctcatg ccgcccatgg cagcggcggg ctggtcgcga tgaccttccc cgggccctac
     4021 cgtctgccgg cctaccgttt caagtcgtcc ggctggttca ccaacacatg cggcgaggga
     4081 ccctatcgcg gcccgtggat gatggacatg ttcgcccgcg agacgatgct cgacaaggcg
     4141 gcccggcaga tgggcgtcga tcctgtcgaa ctgcggcgca agaacatcat cggccggccc
     4201 gaccagccgt tcaccatggt caccggcctc gttctcgatg acgtgacccc gcgcgagacc
     4261 atggacgcca cggtagccgc cattgacgtc cccgccttcc gccgcgagca ggaggaggcc
     4321 cgcaagcagg ggcgctatct cggcctgggc gttgcggcca cgatcgagcc gactgccatc
     4381 gccttcggga cctattcgtc ggaagtcgcc catgtccggg ttgagccgac cggcaaggtc
     4441 acggccatgc tcagcactct ttcgcaaggg cacggcaccg ccaccaccat ggcccagatc
     4501 gtggctgacc ggctgggcgt gcccttcgcc gatgtgacga tcgtcgaggg cgattcccag
     4561 cggaccggct atgggtcggg cgcgggcggc agccgtcagg ccgtcgtcgg cggcggggcc
     4621 gccacggtgg ctgcggaccg cctggccgaa aagatcaagg tcatcaccgc ccacgccttc
     4681 aacgcctcgc ccgaggccgt gcagatcgac aatggccaga tcactatcca gggctcggaa
     4741 atgcgcgcct cgctcgccga aatcgcggcg atggcctatt tcgacaccga ccgcctgccg
     4801 cacggcatgg agagcggtct cgaaacgcaa taccgcttcc gcaccccgcc gatggtcttc
     4861 gccaatgccg cccacgcctg catcgtcgag gtcgatatcg aaaccggcaa ggtcaagatc
     4921 ctgcgctggg tggccggcgg cgattgcggc aacctgatca acccggccat cgtcgagggg
     4981 cagatttcgg gcggcgtggt ccagggcatt gccggggtgc tgttcgagaa ggttgcctac
     5041 gacgccaatg gccagccact cgcggccacg ctgaaggact acctcgtgcc gaccgcgctc
     5101 gacgtgccgc gtctcgaata tgtccacctg tgcacgccgt cgaccacgcc gggcggctac
     5161 aagggcgtgg gcgagggcgg ggcgatgatc gcgccgccaa ccctggtcaa tgcgatcaac
     5221 gatgccctcg cgcctttcgg caagacctgg ttgacaatgc cgctttcgcc cgaccggatc
     5281 gtctgcggcc tggccgacgt cgaggcctga tcggaggggg ccggggcagt tcgatctgcc
     5341 ggcggcgggc ctcacggggc tctgccgcag gcaggggcgg ttcagcgcat tgcagcccag
     5401 ttgcagtccg cttgcgtgag cgacgcgtcg gtttcccaga aatgggcgcc ggcgtgccgc
     5461 gcaagaatgt ccggcggcag ggtcggtcgt gtcggaatcc gggccacagc ctggcggacc
     5521 tgatgcaggc cgggagcccc caacttgtgg tcaaacgcct gttcttcgaa ggtcacattg
     5581 ttgaaatcat gggcgaaaga ttgcagcccg cagaaccggt agatggccga gataacctcg
     5641 gccggagcgg tcaccagcga ctggtatgac accagcatca gccgctcact ggccgctccg
     5701 tagaacgctt ccttgagccc gttaagcgcc acgccgacga gcccgttggc aaacatcaac
     5761 cggtcggtcc gggtatagac cgaggtgtcc ggatcgcagc cgatgatctg cgacagctgc
     5821 agcggatgcc gcaggaactg gtgctcaaag ctctgtaata tctcgccaat cggccgcacc
     5881 aggcagacca gcttggtggc cggaaacagc tccagaagga ccggcaactt ctgcgtccag
     5941 ctgcgattgg tatcgaacac tacctgttca ggccgccccg catagtagct ctcgaacagc
     6001 ccccgcagca cggccgcccg ctcctgatcg gaaaacatca cgccgaattc gttggcgctg
     6061 ctcaggcgcg gcatcagcgc gttgaccagg ctgaataccg ggctgctcat ccccgcgtaa
     6121 aatttgcgga ttctgccgca ggatcgcccc cagcagggtc gatcccgagc gcggcaggcc
     6181 ggagatgaag aaggtctgcc gcatcaatag ggcaggccgt cgcgccgccg gatcgagcgc
     6241 gacaggttgc cgcggtacat gtattgacca agatgcccga gatcggattg gaagtcggca
     6301 tgaaccttgc cgcccatgtc gcgccagcgc ttgcagaagg catagtcttc agaaagatag
     6361 cgcttggttt cgggctcgac catgcaatcg aagaacaacc agtagaactg cttcttggcc
     6421 tgcttttcgg cgccttcggg catgtagttg agctcgggat agtgctccat catgcgatag
     6481 atcacgtcgc gcttgatgca catgaacccg gtcggcgcct cggccacttc gatgaactcg
     6541 tcagcattca ggcggggcac gatcggctct tcccacatgc ctaccgggtt gaacgggaag
     6601 gtggcatgcc gcaccacgta ttcgtcaagg gtcatggcct cggaaatgcc ctcggccggc
     6661 cagttgaggt tcttgatcgg atagatcccc gccaccacgt caaggtcgga gaggagcaag
     6721 cggaaagcgt cgcggggctg gaactcgata tcggaatcga cccacagcag gtgggtaaat
     6781 tcctcatgct caaggaagaa ccggaccatc tcgttgcgcc cgcgggtcac gaggctctcg
     6841 gaatgcatgt gcagcgtggt ctgcaggccg aagcggttgg cagccatgcc gaacctgaag
     6901 atgctcgtta cgtaattcat tgaaacggca gagatatagc acggcgtggc aatcatcacg
     6961 tgcattgact tcagtgcttc atcgggaagc atgcaattcc cttcctcatc cgttctgttg
     7021 ccgtcggcag gacctacggg tctggcctgc gatggacgcg ccgcgcctag tcttagtggc
     7081 aatcgattga ccggcaagtg ccgttaacaa tgaaatcggt cggtcacccg agccggggcc
     7141 tcagtcccca agtaaactgg gtgggccgga caccggcatc cacccaagag caatccccga
     7201 gcgcggcagc gcccggggat cgttctcttc agcccatcgg gctggcggat caggtcgata
     7261 tcagaactgg ataccgaagc ccgccgaagc gcccgtagtg ccctgactgt ccaccgtacc
     7321 gccggcgcgg aagctccact tgccgtcgtc ggtagtcttg gcgagaccca gcgagtagcc
     7381 ggtctggccg tgccagtagc cgaagccgcc actgaacatc caggcacccg gctccgtcgg
     7441 catcggcatg gccgccatcg acatggccga ggcgatcccc gcgctcagct tgcggctgag
     7501 ggtgtcggtg atccagtaga cctggccgcc attcaccgca tccgtgctgg tcggagtgat
     7561 ccggcccgca gcgacgttgg tgatctggcg ctcggcgcct tgcgaaccaa ccgaaaccac
     7621 gctctgcggg ttgttgcccg ggaagttcgt gaagccccag ccgtcgaccg atgccccgct
     7681 ggtcgggttc gacggtgcag tgaccgagtt cgaacccagc gcgacgtcgt tcgggttgtt
     7741 ggcccgcgcg ttgtagccaa ccgccgtgga tccggtcgcg actgctgtcg cggcgaagcc
     7801 gatcgccgtg gactggttgg ccgcggcgtt ggccgtcccg ccgaaggcag aagcggtagc
     7861 gccgttggca gtggcattgt taccgaaggc aattgcctgg gctccgttgg cgaatgcccc
     7921 ggcgcctccg gccgtggccg ccgctccgtt ggcaacagcg aggacgcccg tggctgtggc
     7981 cgaatcgccc agggcctgcg cccggtcgcc gaaggccgaa gccgacgtgc cgcttgcgaa
     8041 tgcctgggtg ccggccgccg aggcattagc cccgaccgc
//
LOCUS       DATNBV010000500         3059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_917192,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000500 DATNBV010000000
VERSION     DATNBV010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917192"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VN627_11870"
     CDS             <1..484
                     /locus_tag="VN627_11870"
                     /inference="COORDINATES: protein motif:HMM:NF023982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXD03993.1"
                     /translation="VAESNPLLIRPAHAEEADAIVALIHRAFGQYRGKLRPESSALQE
                     SADTIRAAMTTGTILLAVRSDRIVGCVSVHRKPDFAYAGRLAVEPMERGVGVGRALMA
                     ETEALARRMGVARLRVDVRLKLRDNRAFFKALGFVEGAHRCHPGFASATYVELEKILI
                     "
     gene            588..941
                     /locus_tag="VN627_11875"
     CDS             588..941
                     /locus_tag="VN627_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HXD03994.1"
                     /translation="MSFGTRVFTWLHGEHVGTDEFGNRYYVDKRTKGKKRERRWVLYK
                     GIPEASKVPPEWHAWLHGTILQPPVSRAPQRAWQKPHEPNLTGTDLAYRPPGHVLKGG
                     ARAPASGDYEPWVPN"
     gene            1008..1469
                     /gene="mlaD"
                     /locus_tag="VN627_11880"
     CDS             1008..1469
                     /gene="mlaD"
                     /locus_tag="VN627_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HXD03995.1"
                     /translation="MERNTLETVLGAVVLLVAGYFLYLVYSQTNIGAEASGYPLELRF
                     DSGGSTGVGTDVRIAGVKVGSVIDQHFDPKTYQAVVTIEMDYAVKLPKDTSAVVTSDG
                     LLGDNYILLNIGGDTEMLAPGERIRNVQGAINLADLINKFVVGTNSGGASN"
     gene            1489..1746
                     /locus_tag="VN627_11885"
     CDS             1489..1746
                     /locus_tag="VN627_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD03996.1"
                     /translation="MSSKGNNKIGRAQHGKGSHVWRQPDGNPVSCVEKIKVLNQNLDE
                     IRQLAQDALEDAILMGCDEAQVRAILQALVEDLQNPYRKDK"
     gene            1773..2201
                     /locus_tag="VN627_11890"
     CDS             1773..2201
                     /locus_tag="VN627_11890"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HXD03997.1"
                     /translation="MALTTLAAPAHAIQMEEAVLQGLDKITARISTIKVAVGETVNFG
                     SLQITLRACDKKPPEETPESAAFLQVVEQKPGEQPVTRFSGWMFASSPALSAMEHPVY
                     DLWVLDCENKGGAAPEQPAEPQQTPAPQAEPAPGQADPAD"
     gene            complement(2202..2864)
                     /gene="aat"
                     /locus_tag="VN627_11895"
     CDS             complement(2202..2864)
                     /gene="aat"
                     /locus_tag="VN627_11895"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HXD03998.1"
                     /translation="MSNRAGSPRITPEMLLRAYAIGIFPMAESRHDPHLYWIDPDARG
                     ILPLDDFHVPRSLKKTVLKGPFQVTADRAFEDVVAGCAEPAPGRRQTWINDTIFELCR
                     KLHVMGYAHSVETWLDGELVGGLYGVALGGAFFGESMFSRETDASKVALVHLVARLKR
                     GGFALLDTQFVTKHLERFGAIEISRDLYRKHLTEALKHRASFPAELPMTALADALNGT
                     AL"
BASE COUNT          591 a          951 c         1030 g          487 t
ORIGIN      
        1 agtggcggag agcaaccccc tcctgattcg accggcgcac gccgaggagg ccgacgcgat
       61 cgtcgcgctg atccatcgcg cctttggaca atatcgcggg aagctgcggc cggaatcgag
      121 cgcgttgcag gaaagcgccg acacgatcag agcggccatg acgaccggca ccatcctgct
      181 tgccgtccgg tccgaccgca tcgtcggctg cgtttcggtc caccggaagc cggatttcgc
      241 ctatgccggc cgcctggcgg tcgagccgat ggagcggggt gtcggcgtcg gccgggccct
      301 gatggccgag acagaggcgt tggcccgccg gatgggggtg gcacgcctgc gcgtcgatgt
      361 ccggctgaag ctgcgcgaca atcgggcgtt cttcaaggcg ctcggattcg tcgaaggagc
      421 ccatcgctgc caccccggat tcgccagcgc gacctatgtc gaactggaga aaatcctgat
      481 ttgacggcta cttggcagcc gccgcgcgac ctcacgtcag cttgccagcg cccggacccg
      541 ccgatatagt ccgcggcaat tcgagatttt gaggccggca cgattccatg tctttcggta
      601 cgcgcgtctt tacctggctc catggcgagc atgtcggcac cgacgagttc ggcaaccgct
      661 actatgtcga caagcgcacc aaggggaaga agcgggaacg gcgctgggtg ctctacaagg
      721 gcatcccgga agccagcaag gtgccgccgg aatggcacgc ctggctgcac ggcacgatcc
      781 ttcagccacc ggtcagccgc gcgccgcagc gcgcctggca gaagccgcat gagccgaacc
      841 tgaccggcac cgacctcgcc taccggccac ccggccatgt cctcaagggg ggagcgcggg
      901 caccggccag cggcgattac gagccctggg tgccgaactg aactcagatc aatggcaagc
      961 gaacagtttg gggctgttcc tgccgtttag gagggaacga gagggccatg gagcgcaaca
     1021 cactcgaaac cgtcctcggc gccgtcgtac tgctggtcgc ggggtacttt ctgtacttgg
     1081 tttattcgca gaccaatatc ggcgcggagg cgagcggata tcccctggaa ctgcggttcg
     1141 atagcggcgg atcgaccggc gtcggtaccg acgtgcggat cgcgggcgtc aaagtgggct
     1201 cggtgatcga ccagcatttc gatccgaaga cctatcaggc ggtggtgacg atcgagatgg
     1261 attatgccgt caagctgccc aaggacactt ccgcggtggt caccagcgac gggttgctgg
     1321 gcgacaacta catcctgctg aatattggcg gcgacaccga gatgctggct ccgggcgagc
     1381 gcatccgcaa tgtgcagggc gccataaatt tggcggattt gatcaacaag ttcgtggtcg
     1441 gcaccaacag cggcggcgcc agcaactagg ccgcgggacg ggatgctcat gtcatccaag
     1501 ggcaacaaca agatcggcag ggcacagcac ggcaaaggca gccacgtttg gcggcagccc
     1561 gacggcaatc ccgtttcgtg cgtcgagaag atcaaggtgc tgaaccagaa tctcgacgag
     1621 atccggcaac tggcgcaaga cgcgctggag gatgccatcc tgatgggctg cgacgaggcg
     1681 caggtgcgcg ctatcctgca ggccctggtc gaggatttgc agaaccccta ccggaaagac
     1741 aaatgagata tctccctggg gtgcttggcg cggtggcttt gaccaccctg gccgcgcccg
     1801 cccatgcgat tcaaatggaa gaggcggttc tgcaaggcct cgacaagatc accgcgcgga
     1861 tctccacgat caaggtggcg gtaggcgaga cggtcaattt cggatcgctg cagatcacgc
     1921 tgcgggcctg cgacaagaaa ccgcccgaag agacgccgga aagcgcggcc ttcctgcagg
     1981 tggtcgagca gaagcctggc gagcaacccg tgacgcgctt ctcgggctgg atgttcgcgt
     2041 ccagccccgc gctctccgcc atggagcatc cagtctacga tttgtgggtg ctggattgcg
     2101 agaacaaggg cggtgcagcg ccggagcagc ctgctgagcc acagcaaacg ccggcaccac
     2161 aggcggaacc cgcacccggc caggcggacc cagccgacta gctagagagc agttccgttc
     2221 agcgcatcgg cgagcgccgt catcggcagc tcggccggga atgaggcgcg gtgcttcagc
     2281 gcttcggtga gatgcttgcg gtagaggtcg cgcgaaatct cgatcgcgcc gaagcgttcc
     2341 aaatgcttgg tgacgaactg cgtgtcgagc agggcgaagc cgccgcgctt cagccgcgcc
     2401 accagatgga cgagggccac cttgctggcg tcggtctcgc ggctgaacat gctttcgccg
     2461 aagaacgcgc cgcccagcgc gacgccgtag aggccaccga ccagttcgcc gtcaagccag
     2521 gtctcgaccg agtgggcata gcccatgaca tgcagcttgc ggcacagttc gaaaatcgtg
     2581 tcgttgatcc aggtctgccg gcggccgggc gccggttccg cgcaacccgc caccacgtcc
     2641 tcgaaggcgc ggtcggccgt cacctggaac gggcccttga gaacggtctt cttgaggctg
     2701 cggggaacgt ggaaatcgtc gagcggcagg atgccgcggg cgtccggatc gatccaatag
     2761 aggtgcgggt cgtgccgcga ttcggccatc gggaaaatcc cgatggcata cgcccggagc
     2821 agcatctccg gcgtgatgcg gggcgaccca gcgcgattcg acatggcgcc tcctccaaca
     2881 acgcaagcct aacgcaaatg acggccgtgg catagtcgcc gcgcaaaggg gtgaacagcc
     2941 ggcaggggaa actatgacgc cgatccgggt tcggatgatg gcggcccatg gcggctggat
     3001 gaacgggcgc atgtgctgca tctgcgcggc acgctgcccg atgccgagcg caagcggtg
//
LOCUS       DATNBV010000501         2757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_918034,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000501 DATNBV010000000
VERSION     DATNBV010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918034"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..2054
                     /locus_tag="VN627_11900"
     CDS             84..2054
                     /locus_tag="VN627_11900"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXD03999.1"
                     /translation="MRITNELAQASDERLIDCLNYADPLILRGLIYQVTGDEELAAMP
                     IEKVPFGYSYLDKLVHEADVARVRAKALAWLQGLRDGDRGEAELGPMERLPRSLEMIY
                     GRAIPERELPMWIEETGLNPLARGLEWKGQPSREQKEAFRVVVIGAGISGISAAVQLK
                     AAGVPFVLIEKNPGVGGTWFENRYPGIRVDSPSRGYLHLFSLEYPQPYSFCPGEENVR
                     YMEWVVEKHGLREHIVFDTEVDSMIWDEGTQTWDIQARGPEGRRSWRGNAVISCVGFL
                     SRPQHVQIEGLESFAGVACHTAQWPKDLDLSNKRVGIVGSGASGYQTTPVIAKVAAHT
                     TLFQRTPSWCYATPSYVSPLPEQTVWVDVNVPYYVNFARFSNGSMYSPDNSIKMTRID
                     PDYEHPLARSAHNLAMRDACVAFINEKLGSRPDLVARMTPAAPPMTSRPIRVDPQDNV
                     YTALMQDNVSLVTDPIERVTPQGVVAGGVEHPLDVLVLATGFKANDFLWPMEIRGRGG
                     VTPEELWAKDGPRAYIGAMLPGFPNLFMAYGPNTNNFGGLQIIALLELEIRFALQCIA
                     GLIENAKRSVEVTEEAYWRYNDELDREETLMIYRDPRVSNYYTNEHGRSCVNGPIDIR
                     RMWHWLRDPAGPPRAERDAGLQPWFGGDLVVA"
     gene            2211..>2757
                     /locus_tag="VN627_11905"
     CDS             2211..>2757
                     /locus_tag="VN627_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD04000.1"
                     /translation="MSETAFGLQGKGALVLGGGQGMGEATAMALARAGCRVAVFDYEL
                     ERADRMVAQIEAEGGSAFSLHGDALDDAVLRTAIADADVKLGGIDVMASIIGMAAWSP
                     LVEMATETWDLDHRRNLRYVFVAAQAVAQHMIDRGRGGAMVCIGSVDGIRSAPYHGAY
                     GAAKAGLIHLVKTMAFEWASSG"
BASE COUNT          521 a          851 c          917 g          468 t
ORIGIN      
        1 aacgaccggg tcccgcgcgt tcaattccaa aggtataccg ggaagaagct cagcctgacc
       61 atgtggagct aaggagagac gacttgcgga tcaccaacga gctggcgcag gccagcgacg
      121 agcgcctgat cgattgcctc aactacgccg atccgctcat cctccgcggc ctgatctacc
      181 aggtcactgg cgacgaggaa ctcgccgcaa tgccgatcga aaaggtaccg ttcggctatt
      241 cctacctcga caaactggtc cacgaagccg acgtggcgcg ggtgcgcgcg aaggcgctgg
      301 catggctgca gggcttgcgt gacggggacc gaggcgaggc cgaactcggt ccgatggagc
      361 gcctgccgcg cagcctggag atgatctacg ggcgggcgat cccggagcgc gaactgccga
      421 tgtggatcga ggagaccggg ctcaacccgc tcgcgcgagg tctcgagtgg aagggccagc
      481 cctcgcgtga gcagaaggaa gcgtttcgcg tcgtcgtgat cggtgccggt atctcgggca
      541 tcagcgcagc ggtgcagctc aaggctgccg gagttccgtt cgtcctgatc gagaaaaatc
      601 ccggggtggg cggcacctgg ttcgagaacc gctacccggg catccgcgtg gactcgccga
      661 gccggggcta cctgcacctg ttcagcctcg aatatccgca gccctacagc ttctgcccgg
      721 gcgaggagaa cgtgcgctac atggaatggg tggtcgagaa gcacggcctg cgcgagcaca
      781 tcgtcttcga caccgaagtc gattcgatga tctgggacga gggcacccag acctgggaca
      841 tccaggccag aggccccgag gggcggcggt cgtggcgcgg caatgccgtg atcagctgcg
      901 tcggctttct ttctcgtccg cagcacgtgc agatcgaagg cctggaaagc ttcgccggcg
      961 ttgcctgtca taccgcgcaa tggccgaagg acctcgatct ctccaacaaa cgggtgggca
     1021 tcgtcggaag tggcgcttcg ggctatcaga cgaccccggt gatcgccaag gttgccgccc
     1081 acacgacctt gttccagcgg actccctcgt ggtgctacgc cacgccgtcc tatgtcagcc
     1141 ccctgccgga gcagaccgtg tgggttgacg tcaacgtgcc gtactacgtc aacttcgcgc
     1201 gcttcagtaa cgggagcatg tacagcccgg acaattcgat caagatgacg aggatcgatc
     1261 cggattacga acatcctctc gcgcgcagcg cccataacct ggctatgcgc gatgcctgcg
     1321 tcgccttcat caacgagaag ctcggcagcc ggcccgatct tgtcgccagg atgacccccg
     1381 ccgcgccgcc gatgacctca cgcccgatcc gggtagaccc gcaggacaac gtctacacgg
     1441 cgctgatgca ggacaatgtc agcctggtga ccgatccgat cgagcgggta acgccccagg
     1501 gcgtggtcgc cggcggtgtc gagcatccgc tcgacgtcct ggtgctggcg acgggtttca
     1561 aggcgaatga ttttctctgg ccgatggaaa ttcgcggacg cggaggggtc acccccgagg
     1621 agctgtgggc gaaggacggg ccgcgcgcct atatcggcgc aatgctgccc ggtttcccga
     1681 atctgttcat ggcctacggg ccgaacacca acaacttcgg cggcctgcag atcatcgccc
     1741 tgctcgagct ggaaatccgc tttgccctgc aatgcatcgc cgggctgatc gaaaacgcaa
     1801 agcgcagcgt cgaagtgacg gaagaggcat actggcgcta caacgacgag ctcgatcgcg
     1861 aggaaaccct aatgatctac cgcgacccgc gggtttccaa ttactacacc aacgagcacg
     1921 gccggtcctg cgtcaatggc cccatcgaca tccgccgcat gtggcactgg ctgcgcgatc
     1981 ccgccggtcc gccccgcgcc gaacgcgacg ctggcctgca gccatggttc ggcggagacc
     2041 tggtcgtcgc ctgatgcggc cgccagagag ccggttgata tgtgatcagg tcggcaaatc
     2101 gatatgccag gctggtatcg actgggtgcg ggcggtggcc gtcaaggtgt tgccgcagct
     2161 gactgatgag cagaagcgcg agtgcgctcg gagtgaagca ggagtggatt atgagtgaaa
     2221 cggcattcgg gctgcaagga aagggtgcgc tggtcctcgg cggaggccag ggcatgggcg
     2281 aggcgactgc catggccctg gcgcgcgcag gctgccgcgt ggcggtgttc gattatgaac
     2341 ttgagcgtgc cgacaggatg gtcgcgcaga tcgaggccga aggcggcagt gccttctcgc
     2401 tccatggcga tgcgctggac gatgccgtac tgcgcaccgc aatcgccgat gccgacgtca
     2461 aactgggtgg cattgatgtc atggccagca tcatcggcat ggcggcctgg agcccgctgg
     2521 tcgagatggc gacggagaca tgggacctcg atcaccggcg taacctgcgc tacgtcttcg
     2581 tcgccgcgca agcagtagcc cagcacatga tcgaccgtgg caggggcggt gcgatggttt
     2641 gcatcgggtc ggtcgacggg atccgcagcg cgccttacca cggcgcttat ggtgccgcga
     2701 aggccggcct catccacttg gtgaagacaa tggccttcga gtgggcgtca agcggta
//
LOCUS       DATNBV010000502         2495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4395069,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000502 DATNBV010000000
VERSION     DATNBV010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4395069"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VN627_11910"
     CDS             complement(<1..288)
                     /locus_tag="VN627_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04001.1"
                     /translation="MTTREGSLEAPTRHPIDWKNPEYYDEAKIVAEAERIFEICHGCR
                     RCVSLCNTFPLLFDKIDGTSDGELAEVPQEAYWDVVDQCYLCDLCYMVKCPY"
     gene            complement(335..757)
                     /locus_tag="VN627_11915"
     CDS             complement(335..757)
                     /locus_tag="VN627_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HXD04002.1"
                     /translation="MKSLKGTKTHDNLKAAFAGESQANRRYLYFANKADVEGQNDVAA
                     LFRSTAEGETGHAHGHLDYLAVVGDPATDLPIGSSRDNLKAAVAGETHEYTDMYPGMA
                     KAARGEGFDEIADWFETLAKAERSHANRFQKALDALTD"
     gene            complement(954..1631)
                     /locus_tag="VN627_11920"
     CDS             complement(954..1631)
                     /locus_tag="VN627_11920"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HXD04003.1"
                     /translation="MRKLVFAIATMFSAAVPVAALAEGYGGNDATPPTYADDQYRYYN
                     RYDNNRAEAYDQYRDNREYRDNGARARVVETRAVTEPGSGREECWNARAGHYEELRTE
                     NNSSSDHKNLAGTVIGGIAGGVLGHQLGSGRGNTAATVGGAALGAYAGNRVENNRHDN
                     DQAQADLDRSNCRTIGTSGNVQAYDVRYNWQGREYVTRMDHDPGRFVQLGRDVNADGT
                     PFALVQR"
     gene            1826..2053
                     /locus_tag="VN627_11925"
     CDS             1826..2053
                     /locus_tag="VN627_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04004.1"
                     /translation="MIRMLAATLAGFALAGCALPQTTADASVPREDHDYITGSRLPAH
                     HNNSSGTKVMTLNPIDRDEMMQPKSNGLNGN"
     gene            complement(2112..>2495)
                     /locus_tag="VN627_11930"
     CDS             complement(2112..>2495)
                     /locus_tag="VN627_11930"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HXD04005.1"
                     /translation="VVGGVLGHQVGNGRGNTAATIAGAAVGGLAGNQYDRSNGGPPPV
                     QTTEIERVPVERNVERCRTVQEVREATVGFDVRYDYNGRQFSTRMPHDPGRFVHVRVD
                     VQPVVEEGAPPPPHVSGGPRPPSYR"
BASE COUNT          370 a          818 c          860 g          447 t
ORIGIN      
        1 gtacggacac ttcaccatgt agcagagatc gcagagatag cactggtcca ccacgtccca
       61 gtacgcttcc tgcggcacct cggcgagctc tccgtcgctt gttccatcga tcttgtcgaa
      121 gagcagcgga aacgtgttgc agaggctcac gcagcggcgg cagccatggc agatctcgaa
      181 gatgcgctcg gcctcggcga cgatcttcgc ctcgtcgtag tactcggggt tcttccagtc
      241 gatcgggtgg cgggtagggg cttcgagact gccttcgcga gtggtcatga ggtaaggaag
      301 cgcaaggctt gcgccctgcg ccctaagccc tggcttagtc cgtcagcgca tccagcgcct
      361 tctggaaacg gttcgcatgc gagcgctccg ccttggcgag cgtctcgaac cagtcggcga
      421 tctcgtcgaa gccttcgccg cgcgcggcct tggccatgcc gggatacatg tcggtgtact
      481 cgtgcgtctc gccggcgacc gcggccttga ggttgtcgcg cgagctgccg atcgggaggt
      541 cggtcgccgg gtcgcccacg accgcgaggt aatcgaggtg gccgtgcgcg tggcccgtct
      601 cgccctcggc ggtggagcgg aagagggcgg cgacgtcgtt ctggccttcc acgtccgcct
      661 tgttggcgaa atacaggtag cggcggttgg cctgggattc gccggcgaag gcggccttga
      721 ggttgtcgtg ggtcttcgtg cctttgaggg atttcattgg agcctccttg ggggaacgga
      781 cgaaacggaa acgccggagc aggacgaatg cctgctcgct gctccccaga ctagccccgg
      841 gccgcagcaa gcgtccaatt gattgtatcg atccgcccga ttggctgcgc caatggcagg
      901 aaaaacaaaa gcccggagcg ggaagccccg ggctttttcc ggcgggaacg agcttagcgc
      961 tgcaccagcg cgaacggcgt tccgtccgcg ttcacgtcgc gcccgagctg cacgaaacgg
     1021 cccggatcgt ggtccatgcg cgtcacgtac tcgcgaccct gccagttgta gcgcacgtcg
     1081 taggcctgca cgttgcccga ggtgccgatc gtgcggcagt tcgagcggtc gaggtcggcc
     1141 tgcgcctggt cgttgtcgtg gcggttgttc tcgacgcggt tgcccgcata ggcgccgagg
     1201 gccgcgccgc cgacggtggc ggccgtgttg ccgcgcccgc tgccgagctg gtggccgagc
     1261 acgccgcccg cgataccgcc gatgacggtg ccggcgaggt tcttgtggtc gctgctgctg
     1321 ttgttttccg tgcgcagctc ctcgtagtgg cccgcgcggg cattccagca ctcctcgcgg
     1381 ccgctgcccg gctccgtcac ggcgcgggtt tcgaccacgc gcgcacgcgc gccgttgtcg
     1441 cggtactcgc ggttgtcgcg atactggtcg taggcttccg cacgattgtt gtcgtagcgg
     1501 ttgtagtagc ggtactggtc gtccgcatag gtgggcggcg tggcgtcgtt gccgccgtag
     1561 ccctcggcga gcgccgcaac cggcaccgcc gcgctgaaca tcgtcgcgat cgcgaagacg
     1621 agttttctca tatgtcctcc aaggggaaat gcctatcccc aatggacacg gcccgggccc
     1681 ggcatgttcg gcgcggggat gatcatatgg gcgtaggagg gcgcgtattc gggccccggc
     1741 cggcgcctac gcccgtaatc cggcttcctt tgcgacctga ccgccctgcg ataccatggc
     1801 ggctttacac ccgaaaggta cgtgaatgat tcgcatgctc gccgcgacgc tggccggctt
     1861 cgcccttgcc ggttgcgccc ttccccagac caccgccgat gcttccgtgc cgcgcgaaga
     1921 ccacgactac atcacgggca gccgcctgcc cgcgcaccac aacaattcgt cgggcacgaa
     1981 ggtgatgacg ctgaatccca tcgatcgcga cgagatgatg cagcccaagt cgaacggcct
     2041 gaacggcaac tgaagccggc cggagacgaa aaaagcgccc cgcagggcgc ttttttcgtg
     2101 ccggaacccg ctcagcgata ggacggcgga cgcgggccgc cgctcacgtg cggcgggggc
     2161 ggcgcccctt cctcgaccac gggctgcaca tccacgcgga cgtggacgaa ccggcccgga
     2221 tcgtgcggca tgcgcgtgct gaactggcgg ccgttgtagt cgtagcggac gtcgaagccg
     2281 acggtggcct cgcgcacttc ctggacggtg cggcaacgct ccacgttgcg ctcgaccggg
     2341 acacgctcga tctccgtggt ctggacgggg ggcgggccgc cgttggaacg atcgtactgg
     2401 ttgccggcga ggccgcccac ggcggcaccg gcgatggtgg cggcggtgtt gccgcggccg
     2461 ttgccgacct ggtggccgag cacgccgccg acgac
//
LOCUS       DATNBV010000503         5633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1267916,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000503 DATNBV010000000
VERSION     DATNBV010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267916"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="VN627_11935"
     CDS             complement(<1..121)
                     /locus_tag="VN627_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HXD04006.1"
                     /translation="MLARTANGVFWMARYLERAENSARLLDAGFRIALTRGDHA"
     gene            complement(237..1679)
                     /locus_tag="VN627_11940"
     CDS             complement(237..1679)
                     /locus_tag="VN627_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HXD04007.1"
                     /translation="MAKTQAVTYDEMLGAEGAVRAAYADYSGWYEGQDPAWLRKQAVE
                     AESFFRRTGITFNVYGNDAGEERLIPFDMIPRIITAGEWRKLTRGIEQRVRALNAFLH
                     DVYHRQEIIRAGKLPERLLRENEAYLPQMAGFVPPGGVYTHIVGIDLVRTGPDEFMVL
                     EDNARTPSGVSYMLENRETMMAMFPELFSRVRVRPVSDYPRRLARSLAACLPPAAQFT
                     QSERPTVAVLTPGIYNSAYFEHAFLADQMGAELVEGADLRVVDGRIAMRTTRGWQPID
                     VLYRRVDDDYLDPLTFNPSSALGVPGIMDIYRAGGITIANAPGTGIADDKAIYSFMPE
                     IVEFYTGEKPILKNVPTWRCSEADSLAYVLDNLAELVVKEVHGSGGYGMLIGPTSSRK
                     EIAAFAAKLRARPENYIAQPTLALSTCPILTRAGLAPRHVDLRPFVLMSPGGIEITPG
                     GLTRVALKKGSLVVNSSQGGGTKDTWVLED"
     gene            complement(1771..3510)
                     /locus_tag="VN627_11945"
     CDS             complement(1771..3510)
                     /locus_tag="VN627_11945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXD04008.1"
                     /translation="MGLGPRLVLVLALIVAAGLLLLGTVRAQTSGGGELAACVARQHL
                     GDTPRALLSDPGHFDCRKDSSAWGPGSYWVRLAVPATPTAADPANPATILRLVPQWQR
                     SLTVYSRDATGQILSRSFDNAELTPMVGIGGAIGVPLGGQAGPVTDVLLRIDGALNAA
                     GLVGDPRLVSTDRLHADELVATALFAAFAGLGLGLFCYNIVLWLTIRERFQLTYCLSL
                     LAMLAYVWASSGTMAVQFPLIPNDVRMATSYAMLAFVAALALQFVTDIIEPEKVPTGL
                     RLFARHYGLVFVATALSVGLVPDAWRPVAERAFVYSFLPLPPLVLAVTWIAWRRGSRS
                     VRILAIAWALPLVMSVVRIAHALHLIHSTPLVHYSLVIGMSFEALLSSLAMSYRIKLI
                     AAERDRAIIDERAARHLASVDSLTGLLNRRALLEQVIAWGSPEPLRLLIVDVDHFKQI
                     NDTHGHLVGDEVLRAFAEVLAIRADLRASVARLGGEEFALVGTVDELHEALALGILAD
                     TRARAMYGEVRITVSVGMAEGMVRSEDEWRELYRRADAALYQAKAEGRNRAIHALREG
                     PASQPGIGFRAVA"
     gene            complement(3630..4871)
                     /locus_tag="VN627_11950"
     CDS             complement(3630..4871)
                     /locus_tag="VN627_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXD04009.1"
                     /translation="MSEHAALLDSAPVSPVPAHVPPELVWPHDLAAYAASRAVDPFIE
                     VSAELHGGPDIRWTANAHPGRPGWIMTRYDLMQEVMQRPELFSSAKSTNFSELLGVTW
                     RQNPLEIDPPRHMGFRQVLQPWFQPSAVARLEETVAADCAALLDEVIDRGACDFMADF
                     ALLFPSTVFLRLMGLPLDLRDQFLAWEHKIVRGTPPEKIATLRTVTDYLMAMMREKRR
                     NPSQGDLASHIATARIDGELMDEGDMFGMALVLYVGGLDTVASSLGWYFRHLAANPAL
                     QDALRAAPEKIPDAVDEFLRAFGVTSQLREVTADTEFHGVVLRQGDLVSIPTFLASRD
                     ERVYADPHRIDIDRKARHLTLATGPHNCLGIHLAKREIRIVLEQWLARTRNIRLQPGE
                     TASWHTIGVWGVARLPLMWDT"
     gene            complement(4911..5633)
                     /locus_tag="VN627_11955"
     CDS             complement(4911..5633)
                     /locus_tag="VN627_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXD04010.1"
                     /translation="MRLAREPRPAGVRTPPLGLLLRELALLVNRRRGGALPVSSAADP
                     LPVMILPGFGAHPARMRRMGAALEQAGHLVFDWGLGFNLGPTPENFAFLLARVRRIAG
                     ATGRPVALVGWSLGGLFAREIAKLAPGAVDRVITLGTPFSGDPRANNAWRAYQVITGH
                     SVDAPPVGSGDFAAKPPVPTIALWSPRDGVIAPRCAAGWPGERDRAVALRCSHLGFAT
                     SAQAIAAVMAILEWPLAGPDRQ"
BASE COUNT          994 a         1753 c         2065 g          821 t
ORIGIN      
        1 aggcatgatc gccgcgggtg agcgcgatgc ggaaaccggc atcgagcagg cgggcgctgt
       61 tttccgcgcg ctcgaggtag cgggccatcc agaacacgcc gttggcggtg cgagctagca
      121 tgggcgggcg ctccgggcgg ggaggacgtg gcgcgaggct tcaaccattg cgacctgctg
      181 aaacacgttc ggcatgactg gggtcatcga cgcaccggag ggagcagaaa ctaccatcag
      241 tcctccagca cccaggtgtc cttggtgccg ccgccctgcg aagaattcac cacgaggctg
      301 cccttcttga gcgccacgcg ggtcagcccg cccggtgtga tctcgatgcc gccgggcgac
      361 atcagcacga agggcctgag atcgacgtga cgcggagcaa ggccggcgcg ggtgaggatc
      421 gggcaggtgg aaagcgcgag ggtgggctgg gcgatgtagt tttccggccg ggcgcgcagc
      481 ttggcggcga aggctgcgat ctccttgcga gaggaggtgg gcccgatcag catgccgtag
      541 ccgcccgacc cgtggacttc cttgaccacc agttcggcca gattgtcgag cacgtaggcg
      601 agactgtcgg cctccgagca gcgccaggtg ggaacgttct tgaggatcgg cttctcgccg
      661 gtgtagaatt cgacgatctc cggcatgaac gaatagatcg ccttgtcatc agcaatgccg
      721 gtgcccgggg cattggcgat ggtgatcccg ccggcacggt agatgtccat gatcccgggc
      781 acgccgagcg ccgaggaggg attgaaggtg agcggatcga gataatcatc gtccacccgg
      841 cgatagagca cgtcgatcgg ctgccagccg cgggtggtgc gcatggcgat gcgtccatcg
      901 acaacccgca gatcggcgcc ttcgaccagt tcggcgccca tctgatcggc caggaaggcg
      961 tgttcgaaat aggccgagtt gtagatgccg ggcgtgagca cggcgacggt gggccgttcg
     1021 gactgggtga actgggccgc gggcggcagg caggccgcga ggctgcgggc gagccggcgc
     1081 gggtagtcgc tcaccgggcg gacccggacc cggctgaaca gctcggggaa catggccatc
     1141 atcgtctcgc ggttctccag catgtaggag acgccggagg gggtgcgggc attgtcttcc
     1201 agcaccatga attcatcggg cccggtgcgg acgagatcga tcccgacgat gtgggtatag
     1261 acgccgccgg gcggaacgaa gccggccatc tgcgggagat aggcctcatt ctcgcgcagc
     1321 agccgctcgg gcagcttgcc ggcgcgaatg atttcctggc gatgatagac atcgtgcagg
     1381 aaggcgttga gcgcgcggac ccgctgctcg atcccgcggg tgagcttgcg ccattcgccg
     1441 gcggtgatga tccgcgggat catgtcgaag gggatcaggc gctcttcgcc ggcatcgttg
     1501 ccataaacgt tgaaggtgat gccggtgcgg cggaagaagc tttcggcctc gaccgcctgc
     1561 ttgcgcagcc atgccggatc ctgcccttcg taccagccgg aataatcggc ataggcggca
     1621 cgaacggcac cttcggcgcc gagcatttca tcgtaggtaa ctgcctgtgt cttggccatg
     1681 gacccccgct attcgcaaag gtctgcaaca ggattcgcca atcgccaagc gaaaaatgct
     1741 gcacttgcga agggtgttgc cacctggcgc tcaggcgacc gcgcgaaagc cgatgcccgg
     1801 ctggctggcg ggcccttcgc gcagggcatg gatcgcccgg ttccggcctt cggccttggc
     1861 ctggtaaagc gccgcgtcgg cccggcggta aagttcgcgc cactcgtcct cgctgcgcac
     1921 catgccttcg gccatgccga cgctgaccgt gatccgcact tcaccgtaca tggcccgcgc
     1981 tcgggtgtcg gcgaggatgc cgagggcgag ggcttcgtgc aattcatcaa ccgtgccgac
     2041 gagggcaaac tcctcgccgc ccagccgcgc gacgctggcg cgcaggtcag cccggatggc
     2101 gaggacttcg gcgaaggccc gcaggacctc gtcgccgacg agatggccgt gggtgtcgtt
     2161 gatctgcttg aaatgatcga catcgacgat gaggagacgc agcggctcgg gcgagcccca
     2221 ggcgatcacc tgttcgagca gggcgcggcg gttgagcagg ccggtgagcg aatcgacgct
     2281 ggcgaggtgg cgcgcggcgc gctcgtcaat gatggcccgg tcgcgctcgg cggcgatgag
     2341 cttgatccgg taggacatgg cgagcgagga cagcagggcc tcgaagctca tcccgatcac
     2401 cagcgaataa tggaccagcg gcgtggagtg tatcaggtgc agcgcatggg caatgcggac
     2461 cacggacatg accagcggca gggcccaggc gatggccagg atccgcaccg accggctgcc
     2521 gcgccgccag gcgatccagg tgacggcgag caccagcggc ggcaggggca ggaagctgta
     2581 gacgaaagcg cgttcggcca cagggcgcca ggcatcggga accaggccga cagacaaggc
     2641 ggtggcgacg aagaccagtc cgtaatgacg cgcgaacagg cgcaggccgg tcggcacctt
     2701 ctcgggctcg atgatgtcgg tgacgaactg gagggcaagg gcggcgacga aggccagcat
     2761 ggcatagctc gtcgccatgc ggacatcgtt ggggatcagc gggaactgca ccgccatggt
     2821 ccccgaactt gcccagacat aggcgagcat ggcgagaagc gagaggcaat aggtcagctg
     2881 gaaccgctcg cggatcgtca gccacaggac gatgttgtag cagaacaggc cgaggccgag
     2941 cccggcaaag gcagcgaaca gggccgtggc gaccagttcg tcggcatgca gccggtcggt
     3001 gctgacgagg cgcggatcgc cgacaaggcc ggccgcgttg agcgcaccgt cgatccgcag
     3061 cagcacgtcg gtgacgggcc cggcttggcc gccaagcggc acgccaatgg caccgccgat
     3121 gccgaccatg ggcgtgagct cggcattgtc gaagctgcgg gacaggatct gaccggtggc
     3181 atcgcgcgaa tagacggtaa ggctgcgctg ccattgcggc accaggcgca agatggtggc
     3241 cgggttggcg ggatcggcgg cagtgggggt ggccgggacg gccagacgca cccagtagct
     3301 gcccggcccc caggcggagc tgtccttgcg gcaatcgaag tggccgggat cggacaagag
     3361 ggcccggggc gtgtcgccca ggtgttgccg ggcgacgcag gcggccagtt cgcccccgcc
     3421 actggtttgc gcgcggaccg tgcccagcag gagcagcccg gccgccacga tcagcgccag
     3481 gaccagcacg agccggggcc cgagccccat gcggatcgga gtaaccgatg ccttcatcgc
     3541 cgcgaggctt acctcacaaa cgataaaatt cttttatctc cggcaggtgt gacttgcgga
     3601 ggtcttcaga gggaagagca gaacggcgat caggtatccc acatcagcgg caggcgggcg
     3661 acgccccaga cgccaatagt gtgccacgag gccgtttcgc cgggttggag gcggatgttg
     3721 cgggtccgcg ccagccactg ttcgagcacg atgcggatct cgcgcttggc gagatggatg
     3781 cccaggcaat tgtgcgggcc ggtggcgagg gtcaggtggc gggccttgcg gtcgatgtcg
     3841 atccggtggg gatcggcata gacgcgctcg tcacggctgg cgaggaacgt ggggatcgag
     3901 acgagatcgc cctggcgcag gacgaccccg tggaactcgg tatcggcggt cacctcgcgc
     3961 agctgcgagg tcaccccgaa ggcgcggagg aattcatcca ccgcgtcggg gatcttctcc
     4021 ggcgcggcgc gcagggcgtc ctgcagggcg ggattggcgg cgaggtggcg gaaataccag
     4081 cccaggctgc tggcgacggt gtccagcccg ccgacgtaga gcaccagggc catgccgaac
     4141 atgtcgccct cgtccatcag ctcgccgtcg atccgggccg tggcgatgtg gctggcgaga
     4201 tcgccttgcg agggattgcg gcgcttctcg cgcatcatcg ccatcaggta gtcggtgacg
     4261 gtgcgcaggg tcgcgatctt ctccggcggc gtaccgcgca cgatcttgtg ctcccaggcc
     4321 aggaactggt cgcgcaggtc cagcggcagg cccatcagcc ggaggaacac ggtggacggg
     4381 aacagcaggg cgaaatcggc catgaagtcg caggcgccgc gatcgatcac ctcatcgagc
     4441 agggccgcac aatcggcggc gacggtctcc tccagcctgg cgacggcgct cggctggaac
     4501 cacggctgga gcacctggcg gaagcccatg tggcgcggcg gatcgatctc cagcggattc
     4561 tgccgccagg tgaccccgag caactcgctg aaattggtgg acttcgcgct ggaaaacagt
     4621 tccgggcgct gcataacttc ctgcatcaga tcgtagcggg tcatgatcca ccccgggcgg
     4681 ccgggatggg cattggcggt ccagcggatg tcgggcccgc catgcagttc ggccgaaacc
     4741 tcgatgaagg ggtcgaccgc gcgcgaggcg gcataggcgg caaggtcatg cggccagacg
     4801 agttcgggcg gaacgtgggc gggaacgggc gagaccgggg cggaatcgag gagggcagcg
     4861 tgctcgctca tggcatccac tcacatgttg atggcttatg taggcaccta ctattgccga
     4921 tccggccccg caagcggcca ttcgaggatc gccatcacag ccgcgatggc ttgcgccgaa
     4981 gtggcgaatc ccaggtggct gcaccgcagg gccacggccc tgtcgcgctc gcccggccag
     5041 ccggcggcgc agcgcggggc gatgaccccg tcgcgcgggc tccacagggc gattgtcggc
     5101 accggcggct tggcggcgaa atcgcccgag ccgaccggcg gcgcatcaac cgaatggccg
     5161 gtgatcacct gataggcacg ccaggcgttg ttggcccgcg gatcgccgga gaaaggcgtg
     5221 cccagggtga tcacccgatc gaccgcgccg ggcgccagct ttgcgatctc gcgcgcgaac
     5281 agcccgccga gactccaccc gacgagcgcg accggccgcc ccgtggcccc cgcgatgcgg
     5341 cgcacccggg cgaggaggaa agcgaagttt tcgggggtgg gcccgaggtt gaagccgagg
     5401 ccccaatcga acacgaggtg tcccgcctgc tcgagcgccg cgcccatgcg ccgcatgcga
     5461 gccgggtggg cgccaaagcc gggcaggatc atcaccggca ggggatcggc cgcggagctg
     5521 accggcaagg ctccgccccg gcggcgattc accagtaggg cgagttcgcg caggaggagg
     5581 ccgagcggcg gggtgcgcac gccggcgggg cgcggctcgc gcgccaggcg cac
//
LOCUS       DATNBV010000504         5327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4752770,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000504 DATNBV010000000
VERSION     DATNBV010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4752770"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VN627_11960"
     CDS             complement(<1..1034)
                     /locus_tag="VN627_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXD04011.1"
                     /translation="MLVATHASAQALVQPAPAVPAGQTLNAALGRLARDPRDLAALID
                     AGNAALQMGDVDAAIGFFARADQLSPNNMRVKAGLAGALVRSENPYDAIPLFAEAERA
                     GAVDPVLAVDRGLAYDLVGDNASAQRYYRQALAAGPNDEAVRRLALSQAIAGDRKGME
                     ATLSPLLQRQDKGAWRARAFALAILGAPEEGVAIANQVMPAELANGIAPYLRYMPRLT
                     AAQQAAAANLGRFPRAADVGRDDPRVALYAPPRPVAGGVGSALVPSGEPLGRGRSRQP
                     EPRQPVRTAVAPTPPPVRTAPPEIAPTRSDLPAQRPSGMMAVAAAVSPPAPAPVPAPA
                     RAAVPAQPPVA"
     gene            complement(1225..2742)
                     /gene="ftsZ"
                     /locus_tag="VN627_11965"
     CDS             complement(1225..2742)
                     /gene="ftsZ"
                     /locus_tag="VN627_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXD04012.1"
                     /translation="MSINIGPPAVEELRPRITVIGVGGAGGNAIANMIHAEIEGVDFI
                     VANTDAQALNNAVAEHRIQLGPDITQGLGAGSRPEVGRAAAEETIAEIERALDGVHMC
                     FIAAGMGGGTGTGAAPVIAKTARDKGVLTVGVVTKPFLFEGTRRMRSADAGIEELQKH
                     VDTLIVIPNQNLFLVAKAETTFKEAFQLADEVLQQGVRSITDLMVMPGLINLDFADVR
                     SVMGEMGKAMMGTGEADGPNRAMEAAERAISNPLLDGVSMQGAKGVIISIIGGEDMKL
                     LEVDEAANHIRDLVDPNANIIWGSAFNPDLQGKIRVSVVATGIEQTEQQQQEISRGGF
                     SLGSGRGGIARPGMARSGEPEAAPAPIAAPAPEPIGAEPEPAPAPVAAAPEPVAYEPE
                     AVEAEPLELELDAVADPEGGDAGQADELLLDAGRLADDQPAEPAAEEPLGRRRPLVSE
                     PEERTPRLSGGGSTLFERMANLSRGGGRKDDSDGDDDGDGPAISIPRFLGRQNNQ"
     gene            complement(2887..4167)
                     /gene="ftsA"
                     /locus_tag="VN627_11970"
     CDS             complement(2887..4167)
                     /gene="ftsA"
                     /locus_tag="VN627_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXD04013.1"
                     /translation="MAPPRLTRVISAVNIGSFRISAIIAGITETGELMVLGSAHRAAQ
                     GVKRGYVTDMSAATYAVRDAIERAEKQAQTNVGPVLIATAGAGLVSQVAQVEIEIGGR
                     RIEQDDIDHLLVAGREVIQPDGRMVLHAQPAHYTLDGAHGVANPKGLHAERLGVDIHV
                     MLADGAPIRNLTESVQQAHLAVEAVVGSPIAAGLACLTPEERDLGVALVEFGADVTNV
                     SVHAGGMLLGLASIPLGSADITDAVASHFGIRRFQAERLKCVNGSALASPADHRELIT
                     INGPGEETTGPLARHADEQNRIPRAELVSVITGQLGRIMEDVNRALKSLGFTGQRGQQ
                     VVFTGGGAELVGLADYAQAALGKPVRIGRPAQLPGLPEAYVRPGFATLAGMVLFAAAD
                     PVDIRAVGPRYRAGEQSGGMGLLSRIYRAVLEYF"
     gene            complement(4167..5210)
                     /locus_tag="VN627_11975"
     CDS             complement(4167..5210)
                     /locus_tag="VN627_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HXD04014.1"
                     /translation="MSQTLKRKAPSVRQVAAQQGKARKAKEARARTGSVLDAVMSWLP
                     FSDEQLHRIFLGLIFALAIAGVWGVALMAGVPTLARDQFGMVAANAGFAVHRVDVRGV
                     NHLNGLKVYETALVQKDQALPLVDIDALRANLLQLSWVEDARVSRQLPDTLVIDIVER
                     KPHAVLRKPDKLVLIDALGTELEPIAPERAKGWLIVSGPGAGQQIEALGTLLEAAPAL
                     KPQVKEAEWVGNRRWNLTFKTDQVLALPEGDKAAAGSLMAFARLDGVNRLLGGRVAAF
                     DMRAPDRIYFRVPGRTEDEAKAAAAAAAAKVAAAADRADKAASSGAAEKPVEASRSPA
                     KDATRKPAKREDT"
BASE COUNT          809 a         1836 c         1834 g          848 t
ORIGIN      
        1 gcgaccggcg gctgcgccgg aacggcagct cgcgcaggcg ccgggacggg cgcgggagcc
       61 ggcggcgaaa cggctgcagc aaccgccatc atgccgctgg gacgctgcgc cggcaggtca
      121 gagcgggtcg gcgcgatctc cggaggagcg gtacgcacgg gtggcggcgt gggtgcgacg
      181 gcggtgcgga ccggctggcg cggctcgggc tggcgcgagc ggccgcgacc cagcggttca
      241 ccgctaggca cgagggcaga gccgaccccg ccggccactg gcctcggcgg cgcatagagc
      301 gccacgcggg gatcgtcgcg gccgacatcg gccgcgcggg ggaaccggcc gaggttggcg
      361 gcagccgcct gctgcgcggc agtcagccgc ggcatgtagc gcaagtaggg cgcgatgcca
      421 ttggcaagtt cggccggcat gacctggttg gcgatcgcca cgccttcttc cggcgcgccg
      481 agaatggcga gagcgaaggc acgggcgcgc catgccccct tgtcctggcg ctggagcagc
      541 ggcgagagcg tcgcctccat gcccttgcga tcgccggcga tggcctggct cagcgccagc
      601 cgccgcacgg cttcatcgtt gggacctgcc gccaatgcct ggcgatagta ccgctgggcc
      661 gaagcattgt cgccgacaag atcgtaggcc agcccgcgat cgacagcgag aaccggatcg
      721 accgcgcccg cccgctcggc ctcggcgaac agcgggatcg catcataggg gttttcgctt
      781 cgcaccagcg ccccggccag gcccgccttg acccgcatgt tgttgggcga aagctggtcg
      841 gcacgggcga aaaagccgat tgctgcgtca acatcgccca tctgcaaggc cgcgtttccg
      901 gcatcgatga gggccgccag atcgcgcgga tcacgcgcca aacggccaag cgcggcattg
      961 agcgtctgtc ctgcgggaac ggcaggtgca ggttgcacca gggcctgggc gctcgcatgg
     1021 gtagcgacca acatagcccc ggggcccatc actatagcgg ctgccgtgag cacaaatctc
     1081 gatctggtca tgcgccgtcc acgcgtgtca ggttcccgtc cggtcaccgc cggctcttgc
     1141 cgccccgtgc gggcccggtg cgccttcatg gctgaaccgg aaccgaacgg aagcagccgg
     1201 ggccccaccc cggccgaacg cggtttactg gttgttctgg cggcccagga agcgcggaat
     1261 ggagatggcc ggaccatcgc catcgtcatc gccgtcgctg tcgtccttgc gcccgccgcc
     1321 acgcgacagg ttggccatgc gctcgaacag ggtgctgccg ccaccggaca ggcgcggtgt
     1381 gcgctcttcc ggctcggaga ccagcggccg gcgccggccg agcggctcct ctgccgccgg
     1441 ttcggccggt tggtcatcgg caaggcggcc ggcgtcgagc agcagttcgt cggcctggcc
     1501 ggcatcgccg ccctcgggat cggccacggc atcgagctcc agctcaagtg gctcggcctc
     1561 gaccgcttcg ggctcatagg ctaccggttc cggcgcggcg gcgacaggag ccggtgccgg
     1621 ctcgggctct gcgccgatgg gttcgggagc gggagcagcg atcggggccg gagcagcctc
     1681 cggctcgccg ctgcgggcca tgcccgggcg ggcgatgcca ccccggccgc tgcccagcga
     1741 aaacccgccg cggctgattt cctgctgttg ctgttcggtc tgctcgatcc cggtggcgac
     1801 caccgacacg cggattttgc cctggagatc aggattgaag gctgagcccc agatgatgtt
     1861 ggcgttggga tcgaccaggt cgcggatgtg gttagccgct tcgtcgacct cgagcagctt
     1921 catgtcctcg ccgccgatga tcgagatgat cacgcccttg gcgccctgca tcgagacgcc
     1981 atctagcagc gggttggaga tggcgcgctc ggccgcttcc atcgcccggt tcgggccatc
     2041 ggcctcgcca gtgcccatca tcgccttgcc catctcgccc atgaccgagc gcacgtcggc
     2101 gaagtcgagg ttgatgaggc cgggcatgac catgagatcg gtgatcgaac ggacgccctg
     2161 ctgcagcacc tcgtcggcca gctggaaggc ttccttgaag gtggtttccg ccttggccac
     2221 caggaacagg ttctggttgg gaatgacgat caaagtatcg acgtgcttct gcagttcctc
     2281 gatgccagca tcggccgagc gcatccggcg ggtgccctcg aacaggaacg gcttggtcac
     2341 cacgccgacc gtgagcacgc ccttgtcgcg cgccgtcttg gcgatgacgg gagccgcgcc
     2401 ggtcccggtt ccgccgccca tgccggcggc gatgaagcac atatggacgc cgtcgagcgc
     2461 gcgctcgatc tcggcgatgg tttcctcggc cgcggcccgg ccgacctcag gccgcgaacc
     2521 ggcgccgagc ccctgggtga tatccgggcc caactggatg cgatgctcgg ccaccgcatt
     2581 gttcaacgcc tgagcatcgg tgttggcgac gatgaaatcc acgccctcga tctcggcatg
     2641 gatcatgttg gcgatcgcat tgccgccggc tccgcccacg ccgatgacgg tgatccgggg
     2701 gcgtagttcc tcgactgccg gcggtccgat attgatgctc atcgcggtct ccaacgggcc
     2761 gctgcagccg gcccttcgcc atgtttaaga ttcacttcgc ttgatgcacc ttcagcaact
     2821 ggcgaccaag ggcgcccaca gctttgtcca cagccacaaa tgcaagtttt tggctaagta
     2881 attaccctag aaatactcga gcactgcccg atagatccgg ctcagcaggc ccattccacc
     2941 cgattgctcg cccgcccggt agcgtggacc gaccgcacgg atatccacag gatcggccgc
     3001 cgcgaacagg accatgcccg ccagcgtggc gaaaccaggc cgaacgtagg cttccggcag
     3061 gcccggcaat tgcgccggtc ggccgatccg caccggcttg ccgagcgccg cctgagcata
     3121 gtcggccagg cccaccagtt ccgccccgcc accggtgaac acgacctgtt ggccgcgctg
     3181 gccggtaaag ccgagcgact tcaacgcccg attcacgtct tccatgatcc gcccgagctg
     3241 gccggtgatg accgacacca gctccgcccg cgggatgcgg ttctgctcgt cggcatgacg
     3301 ggcgagcggc cccgtggttt cctcaccggg gccgttgatc gtgatcagct cccggtgatc
     3361 ggccgggctc gccagggccg agccgttgac gcatttcaac cgctcggcct ggaaccggcg
     3421 gatgccgaaa tgcgaggcta cggcatcggt gatatcggca gagcccagcg ggatagacgc
     3481 gaggccaagc agcatcccgc cggcgtggac cgagacattg gtcacatcgg cgccgaattc
     3541 gaccagggcg acgccgagat cgcgctcctc gggggtgagg caggcgaggc ctgcggcaat
     3601 cggcgagccg accacggcct cgaccgcgag atgcgcctgc tggaccgatt ccgtcaggtt
     3661 gcggatcggc gcgccatcgg cgagcatgac gtggatatcg acgcccagcc gctcggcgtg
     3721 gaggcctttg gggttggcca cgccgtgagc gccatccagc gtatagtgcg ccggctgggc
     3781 gtggaggacc atgcggccat caggctggat gacctcgcga ccggcaacca gcaggtgatc
     3841 gatatcgtcc tgctcgatcc ggcgcccgcc aatctcgatc tcgacctggg cgacctggct
     3901 taccagtcca gcaccggccg tggcgatcag gaccgggccg acattcgtct gcgcctgctt
     3961 ctccgcccgc tcgatggcgt cacgtacggc ataggtcgcg gcggacatgt cggtcacgta
     4021 gccgcgcttc acgccctgcg cggcgcggtg ggcggagccg agcaccatga gctcgcccgt
     4081 ctcggtgatg ccggcgatga tcgcggagat gcggaacgag ccgatgttga cggcgctgat
     4141 cacccgggta agccgcggcg gagccatcag gtgtcctccc gcttggcggg tttgcgcgtg
     4201 gcgtccttcg ccggcgacct gctggcctcg accggctttt cggcagcgcc gctagaggcg
     4261 gccttgtccg cccgatcggc tgcggcggcg accttcgcgg cggcggccgc ggcagcagcc
     4321 ttggcctcgt cctcggtgcg gccaggaacg cggaagtaga tacggtcggg cgcgcgcatg
     4381 tcgaaggcgg cgacccgtcc gccgagcagg cggttgaccc cgtcgagccg ggcgaaagcc
     4441 atcagcgagc ccgctgcagc cttgtcaccc tcgggcaggg cgaggacctg atctgtcttg
     4501 aacgtcaggt tccaccgccg gttgccgacc cattcggctt cctttacctg cggcttcagc
     4561 gccggggcgg cttcgagcaa ggtgccgagg gcttcgatct gctgccccgc cccgggaccg
     4621 gagacgatca gccaaccctt ggcccgctcg ggcgcgatcg gctccagctc ggtgccaagg
     4681 gcgtcgatca ggaccagctt gtcgggcttg cgcaggacgg cgtggggctt gcgctcgacg
     4741 atgtcgatta ccagcgtatc gggcagctgc cgcgagaccc gggcatcttc gacccacgac
     4801 agctggagca ggttggcgcg cagggcatcg atgtcgacca acggcagggc ctggtctttc
     4861 tggacgagcg cggtttcata gaccttgagg ccgttgaggt gattgacgcc gcgcacgtcg
     4921 acgcggtgga ccgcgaaacc ggcgttcgcc gcaaccatgc cgaactggtc ccgcgctagg
     4981 gtcggcacgc cggccatcag ggccacgccc caaacgccgg cgatcgcaag ggcgaagatc
     5041 aggccgagga aaatccggtg aagctgctcg tcgctgaacg gcagccagct catcaccgca
     5101 tccaacaccg agccggtccg cgcccgcgcc tccttggcct tgcgcgcctt gccctgctgc
     5161 gcggccacct ggcgcacgct gggggccttg cgcttgagcg tctggctcat gccggggcgg
     5221 cctccccagt ggctttgcgg cgggcggcgg catccatcag cgcctcggcg acaatggctt
     5281 cgaccagatc ctcgtagctc atgccgcaat gcttcgcctg ctcgggg
//
LOCUS       DATNBV010000505         3716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2194309,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000505 DATNBV010000000
VERSION     DATNBV010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2194309"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..520
                     /locus_tag="VN627_11980"
     CDS             20..520
                     /locus_tag="VN627_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HXD04015.1"
                     /translation="MASQPLAPDSDHTFAEARHASAVARGIGRLFARNGIWCMAEMPL
                     RGGRRADLMGIDAKGHVIIVEIKVSRADLLGDAKWPDYLDHCDRFFWGLHPALDRGCM
                     ETEAFRPEACGLIVGDGYDAEILRPAPLLPLAAARRKTEVERLARAALRRQVVGADPH
                     CAAWGD"
     gene            complement(531..692)
                     /locus_tag="VN627_11985"
     CDS             complement(531..692)
                     /locus_tag="VN627_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HXD04016.1"
                     /translation="MREPISPCNRVCRIDQRTGWCLGCRRTLTEIADWPMLTPAEKQA
                     LIDVLDRRI"
     gene            974..2434
                     /locus_tag="VN627_11990"
     CDS             974..2434
                     /locus_tag="VN627_11990"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXD04017.1"
                     /translation="MSYFSELGLAEPILRALETKGYADPTPIQRQAIPALLEGRDLLG
                     IAQTGTGKTAAFALPSLHRLAGNPQQLKPASCRMLVLSPTRELAAQIADNMRGYAKYL
                     RLGVQCVFGGVPVGKQARALTQGTDVLVATPGRLLDLIDQRALTLRHVEIFVLDEADQ
                     MMDLGFIQPLTRVAALLPKDRQSLFFSATMPQAIADLGKRFINDPVRVEVAPQATTAE
                     RVEQYGTLVNQAEKQALLTLTLRAGLADKSIDRALVFTRTKHGADRVVRHLAGAGVSS
                     AAIHGNKSQAQRTRALEGFRQGTVPILVATDIAARGIDVSGVSHVFNFEIPNVPEQYV
                     HRIGRTARAGADGIALSYIAPDERPYIRDIERLTKVKLTPQPLPEDFLKLAAGLPAPK
                     KVDPGEAGADREVRGDRHGQRGGQRAGGRPQGNRDGQRSHGGGHGGGHGGRPQGGRPD
                     GDRAERPEGEARPKRRFRPRGPSGLGQHAGKVRRVS"
     gene            complement(2437..3333)
                     /locus_tag="VN627_11995"
     CDS             complement(2437..3333)
                     /locus_tag="VN627_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HXD04018.1"
                     /translation="MATLPLRSERAAGEQFLSDAFQQQLARWNRARLAPAFPTADWQQ
                     RLADDLTMQRLEGAFLEDLRSEIAPLAAEAPADADDFVAWFGALEATGPGQHDRLHDW
                     LAERASLDEVRWFLRQEAAGEAGFDDLVALTQLKLPDRAKLELARNYWDEMGRGDARG
                     MHGPMLARLTRALKLSPEIETTCWESLALANAMTAMATNRRYAWHSVGALGVIELTAP
                     ARSALTAAALRRLGRSGGERRYFELHAHLDIRHAEAWLAEAIHPLVAEEPARARAIAE
                     GALIRLACGARCYARYRSHFGI"
     gene            3450..>3716
                     /locus_tag="VN627_12000"
     CDS             3450..>3716
                     /locus_tag="VN627_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid hydroxylase family protein"
                     /protein_id="HXD04019.1"
                     /translation="MLIPAAASALAMTAIVAVRYLLASGGFALATRAVRPGLYRGMDR
                     QIRSEIAWSLLSAAIYGLPAGVVAWGWQARGWTRIYADLHAHPLW"
BASE COUNT          547 a         1354 c         1253 g          562 t
ORIGIN      
        1 tatgttctag attgcggaca tggcttccca accgctcgca cccgattccg accacacttt
       61 cgccgaggcg cgccatgctt ccgccgttgc ccgcgggatc gggcggctgt tcgcgcgcaa
      121 tggcatctgg tgcatggccg agatgccatt gcggggcggg cggcgggccg atctgatggg
      181 gatcgatgcc aagggccacg tgatcatcgt cgaaatcaag gtgagccggg ccgatctgct
      241 cggcgatgcc aagtggccgg actatctcga tcattgcgac cggttcttct ggggcctcca
      301 ccccgcgctc gatcgcggct gcatggaaac cgaggcgttc cggcccgagg cctgtggtct
      361 gatcgtgggc gatggctacg atgccgagat cctgcgcccg gcgccgctgc ttccactggc
      421 ggcggcccgg cgcaagacgg aggtggagcg gctggcccgg gccgccctgc gccgccaggt
      481 cgtcggcgcc gatccgcact gcgctgcctg gggcgactag gggcccggcg ttagatacgc
      541 cggtccagca catcaatcag cgcctgcttc tccgctggcg taagcatcgg ccagtcggcg
      601 atctccgtca gcgtacggcg gcagcccagg caccaaccgg tgcgctggtc gatccggcaa
      661 acgcggttgc aggggctgat cggttcgcgc attgcgggat tcaaccacgg cgcgccgtcg
      721 ggggcaaccg gcgcgccacg cgcaatttcc ttgctttatc gcgcaatgct ctttaggggc
      781 ccggtcgtga ccgacgcggg gctccctcgc gcaatcgtcc gccagcgtga gatcggcgcc
      841 ccgaagtgcc tgatccgcgg cgatgcacgg gccacttcac gagcttcccc ttcacgcacg
      901 ggtcgcgaac cgcgaaccca tgcccttccg tgcgaccagc tcgttcgcgc ggcaaatgaa
      961 agccttgaaa tacatgtcct atttttccga actcggcctc gccgaaccga tcctgcgtgc
     1021 gcttgaaacc aagggctacg ccgatcccac gccgatccag cgccaggcca tcccggcgct
     1081 tctcgaaggg cgcgacctgc tcgggatcgc ccagaccggg accggcaaga cggcggcctt
     1141 cgcgctgccc tcgctccatc gcctcgccgg caacccgcag cagttgaagc cggcctcgtg
     1201 ccggatgctg gtgctctcgc ccacgcgcga actcgccgcg cagatcgccg acaacatgcg
     1261 cggctatgcc aagtacctgc gcctcggcgt gcagtgcgtg ttcggcggcg tgccggtcgg
     1321 caagcaggcc cgcgcgctga cccagggcac cgacgtcctc gtcgccacgc ccggccgcct
     1381 gctcgatctg atcgaccagc gcgcgctgac cctgcgccat gtcgagatct tcgtgctcga
     1441 cgaagccgac cagatgatgg acctcgggtt catccagccg ctgacccgcg tcgccgcgct
     1501 cctgcccaag gaccggcaga gcctgttctt ctcggcgacc atgccgcagg cgatcgccga
     1561 tctcggcaag cgcttcatca acgacccggt ccgggtcgag gtcgccccgc aggcgaccac
     1621 ggccgagcgg gtcgagcagt atggcaccct cgtcaaccag gccgaaaagc aggccctgtt
     1681 gaccctcacc ctgcgcgccg gccttgccga caagtcgatc gaccgcgcgc tcgtcttcac
     1741 ccgcaccaag catggggccg atcgcgtggt gcgccacctc gcgggcgccg gggtcagctc
     1801 ggcggcgatc cacggcaaca agagccaggc ccagcgcacc cgcgccctcg aaggcttccg
     1861 ccagggcacg gtgccgatcc tcgtggcgac cgacatcgcc gcgcgcggga tcgacgtgtc
     1921 gggcgtgagc cacgtgttca acttcgagat cccgaacgtg cccgagcagt acgtccaccg
     1981 cattggccgc accgcgcgcg ccggggccga tggcatcgcg ctcagctaca tcgcgccgga
     2041 cgagcggccc tatatccgcg acatcgagcg gctgaccaag gtgaagctca ccccgcagcc
     2101 gctgccggaa gacttcctca agctcgccgc cggcttgccc gcgcccaaga aggtcgatcc
     2161 aggcgaagcc ggggcggacc gcgaagtgcg cggtgatcgt catggccagc gcggcggtca
     2221 gcgcgccggc gggcgcccgc agggcaaccg cgatggccag cgcagccacg gtggtggtca
     2281 cggcggcggc catggtggtc ggccgcaggg tggccgcccg gacggtgacc gcgccgagcg
     2341 ccccgagggc gaggcccggc ccaagcgccg gttccgcccg cgcggcccca gtggtctcgg
     2401 ccagcacgcc ggcaaggttc gccgcgtgag ctgatcctag atcccgaaat ggctgcggta
     2461 gcgcgcatag cagcgcgcgc cgcaggccag ccggatcagc gcgccctcgg caatggcccg
     2521 ggcgcgcgcc ggctcttccg ccaccagcgg gtggatcgcc tcggccagcc acgcctcggc
     2581 gtgacggatg tcgagatggg cgtgaagttc gaaatagcgc cgctcgccgc ccgaccggcc
     2641 cagccgccgc agcgctgccg cggtcagagc cgagcgggcc ggcgcggtaa gctcgatcac
     2701 gcccagtgcg ccgacgctgt gccacgcata gcgccggttc gtcgccatgg cggtcatggc
     2761 attggcgagg gcgaggcttt cccagcaggt cgtctcgatc tcgggtgaca gcttcagcgc
     2821 ccgggtcagc cgcgccagca tcggcccgtg catgccccgc gcgtcgcccc ggcccatctc
     2881 gtcccagtaa ttgcgggcca gttccagctt ggcgcgatcg ggcagcttca actgcgtaag
     2941 tgcgacaagg tcgtcgaacc cggcctcgcc agccgcctcc tggcgcagga accagcgcac
     3001 ctcatcaagg ctcgcccgct ctgcgagcca gtcgtgcagc cgatcgtgct ggccaggccc
     3061 ggtcgcttcc agcgctccga accacgcaac gaaatcgtcg gcgtcagccg gtgcttcggc
     3121 ggcgagcggc gcgatctcgg agcgcaggtc ttcgaggaac gcgccttcga ggcgctgcat
     3181 cgtcaggtcg tcggcaagcc gttgctgcca gtcggcggtg gggaaggcgg gggcaaggcg
     3241 cgcacggttc catcgcgcca gctgctgctg gaacgcgtca ctcaggaact gctcgcccgc
     3301 cgcccgctcc gatcggaggg gaagggtggc catgaatgtt atctcctttg cgccgataaa
     3361 cgatccgcgc aggggccgcg ttccgaccgc tggtcgcggc aggcctgtcc attcgcccgc
     3421 caatcgggca tgggcggtgg gtggaaggaa tgctcatccc cgcagcggcc tcggcgctcg
     3481 ccatgacggc gatcgtcgcc gtgcgctact tgctcgcgag cggcggcttt gccctcgcca
     3541 cccgcgcggt gcgccccggg ctctatcggg ggatggaccg ccagatccgc agcgagatcg
     3601 cttggtcgct gctctccgcc gcgatctacg gactgccggc gggcgtcgtc gcctggggct
     3661 ggcaggcccg cggctggacc cggatatacg ccgatctcca tgcccatccc ctgtgg
//
LOCUS       DATNBV010000506         4912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4114793,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000506 DATNBV010000000
VERSION     DATNBV010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4114793"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VN627_12005"
     CDS             complement(<1..782)
                     /locus_tag="VN627_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04020.1"
                     /translation="MARAAHLVRTTSLAAAAFAAASSGVVIAGPAAPQFRVGVPAGFD
                     ALAAEQHAVLDIFMGGRQVGTVEAVFAPGQFRFADPAAVAAALPGLAARERVIAALSG
                     QLADNRALACRDRREGAACRPLRPEAVAIVFDESRFRVEVFVNPALLETRAAVAESFL
                     PAPVRGLSLTQGLGLTLAGTAGSRAQLAVADSVLVGLGPARLRADLGWQTGQGAYADT
                     LAVETDRRDRRWTAGLFWAPGTELTGRRKLLGVSLATQFDTRL"
     gene            complement(787..1299)
                     /locus_tag="VN627_12010"
     CDS             complement(787..1299)
                     /locus_tag="VN627_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04021.1"
                     /translation="MAQVSPCAPLRQCSALLLAAAVMAMGSAAQAQQAPQVRIRQLND
                     FVFNTLGAAPVDTSLAGNLCVYSTATSGRYTITARGSGTNGAFTLASGSNALPYEVQW
                     GSAANLSSGSALSPNVAFATTTTNQSNSNCNQPASLTASLIVILRASAEQSVQAGSYS
                     GTLTLLVAPN"
     gene            complement(1390..1962)
                     /locus_tag="VN627_12015"
     CDS             complement(1390..1962)
                     /locus_tag="VN627_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04022.1"
                     /translation="MKLLGQYKGRSLAGLSGTVIAAAAVLASPAHATPVQGSIGATST
                     GSVLITATVPNRALITSLTDVTFTNADPSVAATSSQGPCVWSNTSTRNYTITASGSGA
                     SNAFTIANVSGTSVIPYSVGWANSANASSLTALSAGTASSAVASNATNISCGGSGNST
                     LKVTIGASDLQDMIATQSYTGTLTLVVTPQ"
     gene            2158..2715
                     /locus_tag="VN627_12020"
     CDS             2158..2715
                     /locus_tag="VN627_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04023.1"
                     /translation="MMSLVAPRLKKFSPHALTRRFNVLGAVVATLAWAPGIAAAAQPG
                     ELGAASRARLTISASVAARAEASGVTDASLAPTPGARRATALQRICIRSNSVTGRYDL
                     AATGEADGTGFVMTSADGATVPLTLHWTDAATQSAALLPGVPLEGLTAGREACSAASP
                     GLLAAAAPATRKPVTGLITLMVAPL"
     gene            2805..>4912
                     /locus_tag="VN627_12025"
     CDS             2805..>4912
                     /locus_tag="VN627_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04024.1"
                     /translation="MPIPNTRVFLSSVLSSNLGIRFTPAALGDFASISPGNPVAPWLG
                     STADMSGDGKADLIFGASASDVNGLDSGRVIVTLGGLATGTTNALGAGSQLIIDGAAA
                     GDHAGAAVGGIADLNGDGKGEILIGAPGVDWAGATDAGSAYVVFGRGAGEISLSAIDA
                     GTGGFAIRGEAAGDAAGTTILSVGDMNGDGKADFLVGAPGNDIAALDAGAAYVVWGKD
                     TTTPVNLADVANGIGGFKIQGAAAGDLVGTFLGTVGDMNGDGVNEILIGSPNTGSGKD
                     KGGAVYVVFGKADGAAVDLKNLGANGFVINGIRGDTKTGVRGDNLGDAVTGMGDVNGD
                     GRADILVAAPKADHAYLVFGKASTSSIDLVNVKAGIGGVEIVAEAAKDLDQLSVTGGV
                     DLNRDGIADIVIGAPGASTNGTTTGAVYVIWGGGFSSKIDLSLIAQGIGGAKISGTAD
                     SLTGSAVSIVADQNGDGVADLLIGAPGTSEGAYLLYSPTTWQPDVNVYGTEGADVIGA
                     GSRSGVHVVGEGADNIMALGGNDTISTGGGDDTIDGGAGADSMSGGAGNDSYYVDNAL
                     DVVSEAVGQGTDTVYTSVSYTLGANVENLTLLNGGLTGTGNELANLLTGSNGNDDLNG
                     ATGADTMAGGFGNDTYHVDNAGDVVSEAAGQGTDTVIATISTTLAANVENLSLAAAGL
                     TGTGNELANQITGSAGADSI"
BASE COUNT          810 a         1603 c         1785 g          714 t
ORIGIN      
        1 agccgcgtgt cgaactgggt ggcgagcgaa acgcccagca gcttgcgccg gccggtgagc
       61 tcggtgccgg gcgcccagaa caaaccggcg gtccagcgcc ggtcgcgccg gtcggtctcg
      121 acagccaaag tatcggcata ggcgccctgc ccggtctgcc agccgagatc ggcccgcagc
      181 cgcgccgggc ctaggccgac gaggacagag tcggcaaccg cgagctgcgc ccgcgaaccg
      241 gcggtgccgg cgagcgtcag gccgagcccc tgggtgagcg agagcccgcg caccggggcg
      301 ggcaggaagc tttcggccac ggccgcgcgg gtttccagca gggccgggtt gacgaagacc
      361 tcgacccgga agcggctctc gtcgaacacg atggcaacgg cttcggggcg caggggacgg
      421 caggcggcac cctcgcgccg gtcgcggcag gcgagcgccc ggttgtcggc cagctggccg
      481 gagagggcgg cgatcacccg ctcgcgcgcg gcgaggcccg gcaaggcggc ggccacggcg
      541 gcgggatcgg cgaagcggaa ctggccgggc gcgaagaccg cctcgaccgt accgacctgt
      601 ctgccgccca tgaagatgtc gagcacggca tgctgctcgg cggccagcgc gtcgaacccg
      661 gcgggcaccc cgacccggaa ctgcggcgcc gccgggccag ctatgaccac gcccgacgag
      721 gcagcggcaa aggccgccgc cgccagcgag gtggtccgga caaggtgcgc ggcgcgggcc
      781 atcggctcag ttgggcgcca ccagcagggt gagcgtgccg gaatagctgc cggcctgcac
      841 cgattgttct gcactggcgc gcaggatcac gatcagcgag gcggtgagcg aggccggctg
      901 gttgcagttc gaattggact gattggtggt cgtggtggcg aaggccacgt tggggctgag
      961 cgcgctgccg ctggacaggt tggcggcgga gccccactgc acttcataag gcagggcatt
     1021 gctgccactg gcgagggtga aggcaccgtt cgtgccgctg ccgcgtgcgg ttatcgtgta
     1081 ccggccggac gtcgcggtcg aatagacgca caggttgccg gcgagcgacg tatcgaccgg
     1141 cgcggcgcca agggtgttga acacgaagtc gttgagctgg cggattcgca cctggggggc
     1201 ctgctgggcc tgcgcagccg agcccatcgc catcacggcg gcggccagca gcagcgcgga
     1261 gcattggcgc aacggggcgc acggcgaaac ctgggccatg tcatggtcct gtcgggtgaa
     1321 cggcgagcgc gggaaggccg cgcgggatac cggggcgggc ggctgcgccc gccccggccg
     1381 gtccggctgt tattggggcg tcaccaccag cgtgagcgtg cccgtgtagc tctgggtggc
     1441 gatcatgtcc tgcaggtcgc tggcgccgat cgtcaccttg agcgtggagt tgccgctgcc
     1501 gccgcagctg atgttggtgg cgttggaggc cacggccgac gaggccgtac cggcggacag
     1561 cgcggtcagc gagctggcat tggccgaatt ggcccagccc acgctgtagg gaatcaccga
     1621 agtgccgctg acgttggcga tggtgaacgc gttcgacgcc ccgctgccgc tggcggtaat
     1681 ggtgtagttg cgggtggagg tgttgctcca cacgcagggc ccctgcgagc tcgtcgccgc
     1741 aaccgaagga tcggcattgg tgaaggtcac gtcggtgagc gaggtgatca gcgcgcggtt
     1801 cggcaccgta gcggtgatca ggacgctacc ggtcgaggtg gcgccgatcg agccctgaac
     1861 gggcgtggcg tgggccgggc tggccagcac tgcggcggcg gcgatgaccg tccccgacag
     1921 gcccgcgagg ctgcgtccct tatattgtcc aagcagtttc atgatcttag gtctcctcat
     1981 tccagccccc cacgtccgaa acattcggat cgtgaccgga acgagtgcaa tcgatatcaa
     2041 tggtgtagtt aacgaggtcc taagaggtta atttcttgct ccgtaccagg cctgatgggg
     2101 acattacgga gcttgagctt ggacagaaat ttttccattc aatctgatac atatctcatg
     2161 atgtcgcttg tcgcgcctcg actgaagaaa ttcagcccgc acgccctgac ccggcgcttc
     2221 aacgtgctgg gagctgttgt cgccacattg gcctgggcgc ccggcatcgc agcggcggcc
     2281 cagccgggcg aactgggcgc cgcgtcacgg gcgaggctga cgatttcggc ctccgtggcg
     2341 gcccgcgccg aggcatcggg ggtgacggac gccagcctgg ccccgacgcc gggagccagg
     2401 cgtgcaacgg cattgcaaag gatctgcatc cgcagcaatt cggtaacggg ccggtacgac
     2461 ctggccgcca ctggcgaagc ggacgggacc gggtttgtca tgacctccgc cgacggggca
     2521 accgtgccgc tcaccctgca ctggacagac gccgcaacac agagtgcggc cctgttgccc
     2581 ggtgttccgc tggagggcct gaccgccggc cgcgaagcct gttcggccgc atcgccaggt
     2641 ctgctcgccg ccgccgcgcc ggccacgcgt aaaccggtga cggggctgat caccctgatg
     2701 gtggcaccgc tctagagccg cgatcccgaa attccgaggg cgctgacgga ataatcgccg
     2761 ccgggtcccg ttgagtgggg aacgaccacc ttcgggagct tccgatgccc atccccaaca
     2821 cgcgggtttt tctttcgtcc gtcctgtcga gcaatctcgg catccggttc acgcccgccg
     2881 cgcttgggga cttcgcctcg atctcgcccg gcaacccggt tgccccctgg ctcggctcga
     2941 ccgccgacat gagcggcgac ggcaaggccg acctgatctt cggcgcttcg gccagcgatg
     3001 tgaacgggct cgattccggc cgggtgatcg tcacccttgg cgggctggcg acgggcacga
     3061 ccaacgcgct gggcgcaggt tcgcagctga tcatcgacgg cgcggcagca ggtgaccatg
     3121 cgggtgccgc cgttggcggg attgccgacc tcaacggcga cggcaagggc gagatcctga
     3181 tcggcgcgcc gggcgtcgac tgggccggcg ccaccgacgc gggctcggcc tatgtggtgt
     3241 tcgggcgcgg cgcgggcgag atcagcctat ccgccatcga tgcgggaacc ggcggctttg
     3301 ccatccgcgg cgaagcggcg ggcgacgcgg cggggacgac gatcctctcg gtcggcgaca
     3361 tgaacggcga cggcaaggcc gacttcctgg tcggcgcgcc gggcaacgac atcgccgcgc
     3421 tggatgcggg cgcggcctat gtggtgtggg gcaaggacac gaccacgccg gtgaacctcg
     3481 ccgacgtggc caacgggatc ggcgggttca agatccaggg cgccgcggcg ggcgaccttg
     3541 tcggcacctt cctcggcacg gtcggcgaca tgaacggcga cggggtgaac gaaatcctga
     3601 tcggttcgcc caacaccggc tcgggcaagg acaagggcgg cgcggtctac gtggtgtttg
     3661 gcaaggccga cggcgcggca gtggacctga agaacctggg cgccaacggc tttgtcataa
     3721 acggcatccg cggcgacacc aagaccggcg tgcgcggcga caacctgggc gatgccgtga
     3781 ccggcatggg cgacgtcaac ggcgacgggc gggccgacat cctggtcgcc gcgcccaagg
     3841 cagaccatgc ctatctcgtg ttcggcaagg cgagcacttc ttcgatcgac ctcgtcaacg
     3901 tcaaggcggg gatcggcggg gtggagatcg tggccgaggc ggccaaggac cttgaccagc
     3961 tttccgttac cggcggggtc gacctcaacc gcgacgggat tgccgatatc gtgatcggcg
     4021 cgccaggcgc ttcgaccaat ggcaccacta ccggcgcggt ctacgtgatc tggggcggcg
     4081 gcttcagcag caagatcgac ctcagcctga tcgcgcaagg catcggcggg gccaagattt
     4141 ccggcacggc cgacagcctg accggctctg ccgtgagcat cgtggccgac cagaacggcg
     4201 acggcgtggc cgacctgctg atcggcgcgc ctggcaccag cgagggcgcc tacctgctct
     4261 acagcccgac gacctggcag ccggacgtca acgtctatgg cacggagggc gccgacgtga
     4321 tcggcgcggg ctcgcgctcg ggcgtccatg tggttggcga aggcgccgac aacatcatgg
     4381 cgctgggcgg caacgacacg atcagcaccg gcggcggcga cgataccatc gacggcggcg
     4441 ctggcgcgga ttccatgagc ggcggcgcgg gcaacgacag ctactatgtg gacaatgcgc
     4501 tcgacgtggt cagcgaggcg gtcggacaag ggaccgacac ggtctacacc tcggtgtcct
     4561 atacgctggg cgccaatgtg gagaacctga ccctgctgaa cggcgggctc accgggaccg
     4621 gcaacgagct ggccaacctg ctcaccggct ccaacggcaa cgacgatctc aacggcgcga
     4681 ccggggctga caccatggcg ggcgggttcg gcaacgacac ctaccatgtc gacaatgccg
     4741 gagacgtggt gagcgaggcc gccggtcagg gcaccgacac ggtgatcgcc acgatcagca
     4801 ccacgctggc cgccaacgtc gaaaacctct cgctcgccgc agcgggcctg accggcaccg
     4861 gcaacgagct ggccaaccag atcaccggtt cggcgggggc cgattccatc ga
//
LOCUS       DATNBV010000507         2350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2487270,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000507 DATNBV010000000
VERSION     DATNBV010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2487270"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..308)
                     /locus_tag="VN627_12030"
     tRNA            complement(234..308)
                     /locus_tag="VN627_12030"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(274..276),aa:Asn,seq:gtt)
     gene            complement(408..1190)
                     /locus_tag="VN627_12035"
     CDS             complement(408..1190)
                     /locus_tag="VN627_12035"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HXD04025.1"
                     /translation="MKKDRGKLALLVSDSLKAQAGAESMRQAHDWVSFAEADACVVLG
                     GDGFLLHVLHSMIDHGAVKPCYGINLGTVGFMLNRNSGTRPIAERVARAREVEVSPLA
                     MDAVGRDGQHHRFYAINEVSLLRETRQTAKLEVAVNGKVRMAELAGDGVLVATPAGST
                     AYNLSANGPILPLGCRMLALTPISPFRPRRWRGAILPESAKVSFTVREPDKRPVAAVA
                     DQKEVRDVAEVHITTARDHRLTLLFDPGKTLEERIFAEQFQA"
     gene            1304..>2350
                     /locus_tag="VN627_12040"
     CDS             1304..>2350
                     /locus_tag="VN627_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXD04026.1"
                     /translation="MLAAMNQTRTNPGADERDALTGLPGIAAARARLDGWAGDVPAHG
                     LLLSLPRLPAINLAHGTKTGDGVLAEVAARLAHFAAAEFEGPWFAARAGGSTFLLLAG
                     EPLSRERWELLAHGLAERIARPLPGLAPAVRAGSHIALVRGLPGEGADSLLDRLMQAL
                     GMFEKRGGRRLLWADGTSLRPGLAGPALEAELAGALERGEIEVLFQPQFALVPGAPGH
                     GALTGAEALARWNHPRLGRVGAAALFALAERADLVLPLSAAIAASALSQAARWPGALR
                     LSLNVTSGDIATPDWAERLLDQVAASGFPPGRLTLEVTEEALIADVTAAARILARLAR
                     SGIRLALDDFGAGFC"
BASE COUNT          323 a          771 c          866 g          390 t
ORIGIN      
        1 tcgttcgcgt tggacgacga agctatcatc cccttcgggc ccgcgacgat ctattggccg
       61 ctgcgcacgt tgcccgagat gctgggcggg atgctggacg gttcgcgggc tgcctaaccg
      121 agcggcgcaa tccggcacac cgccgctgcg ttcgcaaagt ggggatgaat gtggggaccg
      181 tcaggtggaa tgccgccgaa taccgcacat ttccgccatc catgaggagg aaatggctcc
      241 ctgagtagga ttcgaaccta cggccgctcg attaacagtc gagagctcta ccgctgagct
      301 atcagggagc agtcccgtct ccgggacagg ggtgcgccta taccagcgcc gattggcttt
      361 gcaagcctat ccggagggaa aacgttgctt tccccaacct cgggtaatca ggcttggaat
      421 tgctcggcga agatgcgttc ctccagggtc ttgccgggat cgaacagcag ggtcagccgg
      481 tgatcgcgcg ccgtggtgat gtggacttcg gcgacgtcgc ggacttcctt ctggtcggcc
      541 accgcggcga ccgggcgctt gtcgggctca cgcacggtga acgagacctt ggcgctttcc
      601 ggcaggatcg caccgcgcca gcggcggggg cggaacgggc tgatcggggt cagcgccagc
      661 atccggcagc cgagcgggag gattgggccg ttggccgaga ggttgtaggc ggtcgatccg
      721 gccggggtgg caacgagcac gccgtcgccg gcgagttcgg ccatgcgcac ctttccgttg
      781 accgccactt cgagcttggc cgtctggcgg gtttcgcgca gcagggagac ttcgttgatg
      841 gcgtagaacc ggtgatgctg cccgtcgcga cccaccgcgt ccatcgcgag gggtgagact
      901 tcgacctcgc gcgcccgtgc cacccgctcg gcaatcgggc gggtgccgga attgcggttc
      961 agcatgaagc cgaccgtgcc aaggttgatc ccgtagcagg gcttcaccgc gccatgatcg
     1021 atcatcgagt ggaggacgtg gagcaggaac ccgtcacccc ccagcacgac gcaggcatcg
     1081 gcctcggcga aggacaccca atcgtgcgcc tggcgcatgc tttccgcgcc ggcctgtgcc
     1141 ttcaggctgt cggagaccag cagggcgagc ttgccgcggt ccttcttcat tgccccacca
     1201 tccttgtggc ccgcattatg catggccgtt cgcgccggtg atatggaggc cggggtggtt
     1261 tggcaattga cgggcttgag attttggcaa ggggtctgcc gctatgctgg cggcgatgaa
     1321 ccagacccgg accaatcccg gcgcggacga gcgcgatgcc ctgaccggcc tgcccggcat
     1381 cgccgcggcg agggcgcggc ttgacggctg ggcgggggac gtgccggctc atggcctgct
     1441 gctctcgctg ccgcgcctgc cggcgatcaa tctcgcccac ggcaccaaga ccggcgacgg
     1501 cgtcctcgcc gaggtcgcgg cccggctggc gcactttgcg gccgccgaat tcgaggggcc
     1561 gtggttcgcc gcacgcgcgg gcgggtcgac ctttctcctg ctcgccggcg agccgctcag
     1621 ccgggaacga tgggaactgc tggcccatgg cctggccgaa cgcattgcca ggccgctgcc
     1681 gggcctcgcc ccggccgtcc gcgccggatc gcacatcgcc ctggtgcgcg gcctgccggg
     1741 cgagggggcc gattcgctgc ttgatcggct gatgcaggcc ctgggcatgt tcgagaagcg
     1801 cggcgggcgg cgcctgctgt gggcggacgg cacgagcctg cgtccagggc tggcggggcc
     1861 ggcgctggag gccgaactgg ccggtgccct cgagcggggc gagatcgagg tgctgttcca
     1921 gccgcagttc gccctcgtgc ccggtgcgcc ggggcacggc gcgctgacgg gggccgaggc
     1981 actggcgcgg tggaatcacc cgcggctggg ccgggtcggg gcagccgccc tgtttgccct
     2041 ggccgagcgg gccgatctcg tcctgccgct gtcggccgcc attgccgcga gcgccctgtc
     2101 ccaggcggcg cgctggccgg gagcgctgcg gctctcgctg aacgtgacct ctggcgacat
     2161 cgccacgccg gactgggcgg agcgcctgct ggaccaggtc gccgccagtg gatttccgcc
     2221 ggggcgcctg acgctggaag tgaccgagga ggcgctgatc gccgatgtga cggcggcggc
     2281 gcgaatcctc gcgcggttgg cacggtcggg catccggctg gcgctggacg acttcggggc
     2341 ggggttctgc
//
LOCUS       DATNBV010000508         2696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_294898,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000508 DATNBV010000000
VERSION     DATNBV010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294898"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VN627_12045"
     CDS             <1..1275
                     /locus_tag="VN627_12045"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HXD04027.1"
                     /translation="RSEFGPLPDQSMHEKTSVPMLIEELYTFLKQADARELGGLFREL
                     DAAKAAGDAVGIHRTLGQIDAFQTHVVPIIADIDAGFGNAEATYLLAKKFIEAGACCI
                     QIENQVSDEKQCGHQDGKVTVPHEDFLAKIRAVRYAFMELGVDDGVIVARTDSLGAGL
                     TKQIAVVHEKGDIGDQYNSFLDCEEVDAANLGHGDVLISRDGKLMRPKRLPSNLFQFR
                     SGTGEDRCVLDCINSLQNGADLLWIETEKPHIGQIGGMVNRIREVIPNAKLVYNNSPS
                     FNWTLNFRQQVFDAWTEAGKDVSAYDRAKLMSVDYDSTELAAEADERIRTFQKDAARE
                     AGIFHHLITLPTYHTAALSTDNLAKEYFGEQGMLGYVKGVQRQEIRQGIACVKHQNMS
                     GSDIGDDHKEYFSGEAALKAGGSANTMNQFAA"
     gene            1328..1489
                     /locus_tag="VN627_12050"
     CDS             1328..1489
                     /locus_tag="VN627_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04028.1"
                     /translation="MSEPARARAVLSTEDFKLIKEAVLFYLKAHEDSPDSVKFSNLYH
                     RLGSATRGK"
     gene            complement(1573..1725)
                     /locus_tag="VN627_12055"
     CDS             complement(1573..1725)
                     /locus_tag="VN627_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04029.1"
                     /translation="MTTPVHRTSELRLLLDQMRLHPSRDWSDTPQRAFILNRMLARES
                     TRKGRA"
     gene            complement(1784..>2696)
                     /locus_tag="VN627_12060"
     CDS             complement(1784..>2696)
                     /locus_tag="VN627_12060"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HXD04030.1"
                     /translation="GAARRTDWRVDLSPGVGLGAAMTIAPATDEDQAALRLAAQVPDT
                     ALCTIVGIDGSYSRRRGAQLAVAPDGRMAGSLADGCLEKELATQAAEARAAGTGPAML
                     RYGAGSPFIDFRLPCGAGLDVLIDPAPDRGALAAVMARLDARQPAALALPAPSPVAER
                     LFVPTLRLVVLGAGPEPEALVRLAAAQGLPAELLVPDRDLALGRAPTVLLDPWTAVVL
                     LFHDHEWEPALADWALASPAFYVGAQGGKAAREARAGRLMAMGHDAASLARLRSPVGL
                     IPNARDANTLALSILAEVVAAYEHLRG"
BASE COUNT          492 a          907 c          906 g          391 t
ORIGIN      
        1 cgctccgagt tcggaccgct gcccgaccag tcgatgcacg agaagacctc ggtcccgatg
       61 ctgatcgaag agctctacac cttcctgaag caggccgacg cccgcgagct gggcggcctg
      121 ttccgtgagc tcgacgccgc caaggccgcc ggcgacgcgg ttggcatcca ccgcacgctc
      181 ggccagatcg acgccttcca gacccacgtc gtgccgatca tcgccgacat cgacgccggt
      241 ttcggcaatg cggaagcgac ctacctgctc gccaagaagt tcatcgaggc cggcgcctgc
      301 tgcatccaga tcgagaacca ggtttccgac gagaagcagt gcggccacca ggacggcaag
      361 gtcaccgttc cgcacgagga cttcctcgcg aagatccgcg ccgttcgcta tgccttcatg
      421 gaactgggcg ttgacgacgg cgtgatcgtc gcccgtaccg actcgctcgg cgccgggctc
      481 accaagcaga tcgctgttgt tcacgaaaag ggcgatatcg gcgaccagta caactcgttc
      541 ctcgactgcg aggaagtcga cgctgccaac cttggtcacg gcgatgtcct gatcagccgc
      601 gatggcaagc tgatgcgtcc caagcgcctg ccttcgaacc tgttccagtt ccgctccggg
      661 acgggcgagg accgctgcgt gcttgactgc atcaacagcc tccagaacgg cgccgacctg
      721 ctgtggatcg agaccgagaa gccgcacatc ggccagatcg gcggcatggt gaaccgcatc
      781 cgcgaagtca tccccaatgc caagctggtc tacaacaact cgcccagctt caactggact
      841 ctgaacttcc gccagcaggt gttcgatgcc tggaccgagg ccggcaagga cgtttcggcc
      901 tatgaccggg cgaagctgat gagcgtcgac tatgactcga ccgagctggc ggcagaggcc
      961 gacgagcgca tccgcacctt ccagaaggac gcggcgcgcg aggcgggcat cttccaccac
     1021 ctgatcaccc tgccgaccta tcacacggcc gcgctcagca ccgacaacct cgccaaggaa
     1081 tacttcggcg agcagggcat gctcggctac gtcaagggcg tgcagcgcca ggagatccgc
     1141 cagggcatcg cctgcgtgaa gcaccagaac atgtcgggtt cggacatcgg cgacgaccac
     1201 aaggaatact tctcgggcga agccgccctg aaggccggtg gcagcgccaa tacgatgaac
     1261 cagttcgcag cctgagcaac acggcacccc tccccgaccg ggagggggcc gatcaaggag
     1321 aagcaagatg agcgaacctg cccgcgcccg cgcggtcctc tccaccgagg acttcaagct
     1381 gatcaaggaa gccgtgctgt tctatctcaa ggcgcatgag gacagcccgg attcggtgaa
     1441 gttctccaac ctctaccacc gcctcggctc ggccacgcgc ggtaagtaag caagttcctg
     1501 gggaggaact tccggcccgt cacgcacctg cgtggcgggc cggttgctgt tggggcggtg
     1561 ttctattgcc gcctaggctc gccctttccg ggtgctttca cgagccagca tgcggttgag
     1621 gatgaaggcg cgctgggggg tatcgctcca gtcgcgcgag ggatgaaggc gcatctggtc
     1681 gagcagcagg cgcagttcgc tggtgcggtg aacgggcgtg gtcatgacag gtccttccgg
     1741 cttgtgccgg aacaacgcgc caaggtcgtg gccggatccg cgatcagccg cgcagatgct
     1801 cgtaggcggc gaccacttcg gcgaggatcg agagcgccag ggtgttggca tcgcgggcat
     1861 tgggaatgag gccgacgggg ctgcgcaggc gggcgaggct ggccgcatcg tgccccatgg
     1921 ccatcaggcg cccggcccgg gcttcccgcg cggccttccc gccttgggcg ccgacgtaaa
     1981 aagcaggtga agcgagggcc cagtcggcga gtgccggctc ccattcgtga tcgtgaaaca
     2041 ggagaaccac cgccgtccac gggtcgagca agacggtggg tgcgcggccg agagccagat
     2101 cgcggtcggg caccagcagc tcggccggca ggccctgggc cgcggcaagg cgcaccaggg
     2161 cttcgggttc gggacccgcc ccgagcacga cgaggcgcag cgtgggcacg aacaaccgct
     2221 cggccacggg cgagggcgcg ggaagcgcga gggcggcggg ttggcgggca tccagccggg
     2281 ccatgacggc cgcgagcgcg ccccgatccg gtgccgggtc gatcagcacg tcgagcccgg
     2341 cgccgcaggg caggcggaaa tcgatgaagg gtgagcccgc gccgtagcgc agcatggcag
     2401 gaccagtgcc ggcagcgcgc gcctcggcgg cctgggtcgc aagttccttt tcaaggcagc
     2461 catcggcaag gctgcccgcc atcctcccat ccggagctac ggccagctgc gccccgcggc
     2521 ggcgcgaata ggagccatcg atccccacga tcgtgcacag tgccgtgtcc ggcacctggg
     2581 cggcgaggcg caaggcggcc tgatcttcgt cggtggcggg cgcgatcgtc atcgccgcac
     2641 ccaagcccac gccgggggaa aggtcaacgc gccaatccgt ccgccgcgcc gcgccc
//
LOCUS       DATNBV010000509         5919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3782371,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000509 DATNBV010000000
VERSION     DATNBV010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3782371"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VN627_12065"
     CDS             complement(<1..311)
                     /locus_tag="VN627_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXD04031.1"
                     /translation="MTELARIRNFSIIAHIDHGKSTLADRLIQFTGGLTEREMSAQVL
                     DNMDIEKERGITIKAQTVRLNYTANDGLTYELNLMDTPGHVDFAYEVSRSLAACEGAL
                     LV"
     gene            complement(367..2496)
                     /locus_tag="VN627_12070"
     CDS             complement(367..2496)
                     /locus_tag="VN627_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXD04032.1"
                     /translation="MDAGTEDHQALLLLRDFEASGQGWFWSTDAAGAITYISASVAQA
                     MGRERGELIGAPFHTLFALERDESEDSARTLPLIFSANKSFSELPVRAAQGTGEIAWT
                     ITGRPQFDGQRRFLGYRGNGVEATERLRSHRAASRLAMYDSLTGLANRHRMGQRLVAT
                     LTAYAASQRPCTVMLIDLDRFKQVNDTLGHPAGDELLKQVAQRLQSVCDKGVDIGRLG
                     GDEFQVLLPDVEDRGRLGELAKRIIQILSQPYQIDGSRCVIGASVGIAVAPYDGMTSD
                     ELIRGADLALYAAKGGGRGQFRFYSSELHETAEQRRRLEEDLRDALAKDQIAMYYQPL
                     VSAKNDTVIGVEALMRWQHPEFGSISPSVFIPIAEESDLVIALGEWALRQACSEAMTW
                     PGNIRVAVNVSPAQFAHPNFIANVTQSLAQSGIAPDRLEIELTESIFVNDSETTERTF
                     AAIKRLGVRLALDDFGTGYSSLGYLKHAPFDKIKIDKGFIHEVTVPGSRNPAIITAIV
                     SLARALDMVTTAEGIETRDELALMRELGVDQIQGWIYSKARTGGEVAEALATGDWVID
                     PQGPAKQRAERRTVLRKIGIVHEDHRYEVMMRNLSRTGAAIEGLINVPVGTQFVVDFG
                     EGQLVVATVRRSDDTAQGLEFEIPLVDDGAGGLCTRHRVAPYVLAAMGMPAPGSGGGP
                     VVALNKGTQINLPRFGTADIAKTIRAA"
     gene            complement(2681..5356)
                     /locus_tag="VN627_12075"
     CDS             complement(2681..5356)
                     /locus_tag="VN627_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXD04033.1"
                     /translation="MLRKPALSFPEAPEPDSRPAPVLAPGRAGGRASTARLRPRRFVA
                     RYAIPLEWWMMAPIMLVAVVIHAWASHSLPPVPSGVAAIVAALLAFAAPLAVRYERGH
                     DCGPVQRWAAIGAVVIAPMFLFGLAMSQWSILAQGAGWQTGPAALVMIGMLVGVLCSG
                     RLPTMVAALLSLWFAHVLVSLEPGTMLALVIGAGSGVYISFRQIELNRMAQDRLEQVE
                     RAQQRAELILSDYESTRQGWFWETDRRGLITYVSRTIGEIVGADEGELVGRPFTDLFL
                     EEGQSGESERTLHFHLNARSSFQDLRLRAAALDGEERWWSVNGLPVYDDFDNFQGFRG
                     SGSDLTERKRHEEQATRLAHYDSLTGLANRLQMSKSLEKILNARAVENRTCAVFLLDL
                     DRFKQVNDTLGHPAGDALLRQVAQRLERVVESPGRVGRLGGDEFQVIIPGRSTRNALA
                     HRAERIIMALAEPYQIDGNRVMIGASVGIAIAPDDGVTSEAIIRNADLALYAAKDGGR
                     GRHHFYAPDLHTDAQERRQLEQDLRDALASGGLQLSYQPVVHTASEKIAGFEALLRWN
                     HPTRGWLSPAKFVPIAEDAGIIAQIGEWALRTACQDLAQWPDSVRVAVNVSPLQFANP
                     ALPAIVTSALASAGVVPERLELEITESVFLNDDEGTDAMFAALKRLGVRLALDDFGTG
                     YSSLGYLKKAPFDKIKIDQSFVRGACEKGSCNGAIIAAIVRLAEALGMETTAEGVETL
                     DELDLVRSLGCSHVQGYIYDKPLSAGEAGERLADGVGVSARGPRSSRPARRTVLRRVL
                     LDHDGHSYEATVRNMSATGALVEGLWNVPEGTVFLVRLGEGVTVPATARWCEDDRVGI
                     EFRERIDPETATRGGIVPTRLVYSAAPDLPQRQFG"
     gene            5497..>5919
                     /locus_tag="VN627_12080"
     CDS             5497..>5919
                     /locus_tag="VN627_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXD04034.1"
                     /translation="MRHLAIIGSGPAGYYTAEAALKRWGEDVQVDVFDRLPVPYGLIR
                     SGVAPDHQSIKAVSKRYEGVALSQNVRFIGNVTVGTDITIGELQGLYDAVILATGAPN
                     DRPLDLPGEHLGNVFGSAAFVGWYNGHPQFADLNPDLSG"
BASE COUNT          969 a         1938 c         2011 g         1001 t
ORIGIN      
        1 acgaggagcg cgccctcgca ggcggcgagg ctgcgggaga cctcgtaggc gaagtcgacg
       61 tggccggggg tgtccatgag gttgagctcg taggtgagcc cgtcgttcgc ggtatagttg
      121 aggcgcacgg tctgcgcctt gatggtgatc ccgcgctcct tctcgatgtc catgttatca
      181 aggacttgcg ccgacatctc gcgctcggtc aggccgccgg tgaactggat cagccggtcc
      241 gccagggtgc tcttcccgtg gtcgatatgg gcgatgatgc taaaattgcg gatgcgggcc
      301 agttcagtca tgcccgcccg ttagcagcgt cgcaattggc tgtcatccgc cgcttggcgt
      361 gtgcgctcag gcggcgcgta tggtcttggc gatgtcggcc gttccgaagc gcggcaggtt
      421 gatctgcgtg cccttgttga gcgccaccac cggcccgccg ccggagcccg gcgccggcat
      481 gcccattgcc gccagcacat agggcgcgac gcggtggcgt gtgcacaggc cgcccgcgcc
      541 gtcatcgacc agcgggatct cgaactcgag cccttgggcg gtgtcgtccg atcggcggac
      601 ggtggcaacg accagctggc cttcgccgaa atcgactacg aactgggtgc cgaccggaac
      661 gttgatcagg ccctcgatcg cggcaccggt gcgcgagagg ttgcgcatca tcacctcgta
      721 gcggtgatcc tcgtggacaa tgccgatctt gcgcagcacg gtgcgtcgct cggcacgctg
      781 cttggccggg ccttgcggat cgatcaccca gtcaccggtg gcgagcgcct cggccacttc
      841 gccgccggtg cgcgccttgg aatagatcca gccctggatc tggtcgacgc caagctcgcg
      901 catcagcgcc agctcgtcgc gggtttcgat cccctcggcg gtggtgacca tgtcgagcgc
      961 gcgggccagg ctgacgatcg cggtgatgat cgccgggttg cggctgccag ggaccgtgac
     1021 ctcgtgaatg aagcccttgt cgatcttgat cttgtcgaac ggcgcgtgct tcaggtagcc
     1081 gagcgaagaa tagccggtcc cgaaatcgtc gagcgcgagg cgcacgccca ggcgcttgat
     1141 cgccgcgaag gtccgctcgg tagtttcgct gtcgttgacg aagatgctct ccgtcagctc
     1201 gatctcgagc cggtccggcg cgatgccgga ctgggcgagc gactgggtaa cgttggcgat
     1261 gaagttcgga tgggcaaact gggccgggct gacattgacg gcgacccgga tgttgccggg
     1321 ccaggtcatt gcctcggaac aggcttggcg cagcgcccat tcaccgaggg cgatcaccag
     1381 atcggactcc tccgcgatcg ggatgaacac gctgggcgag atgctcccga attccgggtg
     1441 ttgccagcgc atcagcgcct ccaccccgat caccgtatcg ttcttcgccg agaccagcgg
     1501 ctggtagtac atcgcgatct ggtccttggc gagggcatcg cgcaggtctt cctcaagccg
     1561 ccggcgctgc tccgcggttt cgtgcaattc acttgaatag aagcggaact gcccgcgccc
     1621 gccacccttc gcggcataga gcgcaaggtc ggcgccgcgg atcagctcgt cactcgtcat
     1681 gccgtcgtag ggtgcgacgg cgatgccgac cgaggcgccg atgacgcagc ggctgccgtc
     1741 gatctggtag ggctgagaga ggatctggat gatccgcttg gccagttcgc caaggcgccc
     1801 gcgatcctcg acatcgggca gcaggacctg gaactcgtcg ccacccagac gaccgatatc
     1861 gacgcccttg tcgcagacgc tctgcaggcg ctgggcaacc tgcttgagca gttcgtcgcc
     1921 ggccggatgg cccagcgtat cattgacctg cttgaaccga tcgagatcga tcagcatcac
     1981 ggtgcagggg cgctgcgatg ccgcataggc ggtgagcgtc gcgaccaggc gctggcccat
     2041 gcggtggcgg ttggcgaggc cggtcagcga atcgtacatc gccagccggc tggccgcccg
     2101 gtgcgagcgc agccgctccg ttgcctccac gccgttgccg cgatagccga ggaagcggcg
     2161 ctggccgtca aactggggac ggccggtgat ggtccaggca atttcgccag taccctgggc
     2221 ggcgcgcacc ggcagctcgg agaagctctt gttggcactg aagatcagcg gcagcgtgcg
     2281 ggcgctgtcc tcgctctcgt cgcgctcgag cgcgaacaga gtgtgaaatg gcgcaccgat
     2341 cagttcacca cgctcgcggc ccatcgcttg agcaacgctg gccgaaatgt aggtgatcgc
     2401 cccggccgca tccgtcgacc agaaccagcc ttgcccggat gcctcgaaat cccgcagcag
     2461 gaggagagcc tggtgatcct cggtacctgc gtccatgcgc gcagcctggg aaaaacccga
     2521 ctgtcctcgc aacatgtcgc cgatccgctt taccacttag cgccgccccg tcagtgcctc
     2581 tgccgcgggg cgatgggccc cttggcagaa ttccttacat cgattgtagt gaaggaagtg
     2641 ataaggccga cggactgccg attggcggac gccggagcca tcagccgaac tgccgctgcg
     2701 gcagatcagg ggcggccgag tagaccaggc gcgtgggcac gatccctccc cgtgtggccg
     2761 tttccggatc gatgcgctcc ctgaattcga tgccgacgcg gtcgtcctcg caccaccgcg
     2821 ccgtggcggg gacggtcacc ccctcaccca gccggacaag gaaaaccgta ccttcgggca
     2881 cgttccacaa gccttcgacg agggccccgg tggccgacat gttgcgcacg gtcgcctcgt
     2941 agctgtggcc gtcgtgatcg agcagcactc gccgaaggac tgtccggcgg gcgggccgcg
     3001 acgagcgcgg gccgcgcgcc gagacgccga cgccatcggc gagccgctcg cccgcctcgc
     3061 ccgccgagag cggcttatcg tagatgtagc cctgaacgtg gctgcagccg agcgagcgga
     3121 caaggtcgag ctcgtcgagc gtttccacgc cctcggcggt ggtttccatc cccagcgcct
     3181 cggcgagccg gacgatcgcg gcgatgatcg cgccgttgca gcttcccttc tcgcaggccc
     3241 cgcgaacgaa agactgatcg atcttgatct tgtcgaacgg cgccttcttc agatagccga
     3301 gcgaggaata gccggtgccg aaatcgtcca gtgccagacg cacgcccagc cgcttgagcg
     3361 cggcgaacat cgcatcggtg ccctcgtcgt cgttgaggaa cacgctctcg gtgatctcca
     3421 gttcgagccg ttcggggaca accccggccg aagcaagagc cgaagtgacg atggccggca
     3481 gggctggatt ggcgaattgc agcggcgaga cattgaccgc gacccgtacg gaatccggcc
     3541 attgcgccaa gtcttggcag gccgttcgca aggcccattc accgatctgg gcgatgatcc
     3601 cggcgtcctc ggcaataggc acgaatttgg cgggactcag ccagccgcgc gtcgggtgat
     3661 tccagcgcag cagggcctcg aatccggcga ttttctcgga ggcggtgtgg accaccggct
     3721 ggtaggacag ctggagcccg cccgaggcca gggcatcgcg aaggtcctgt tcgagctggc
     3781 ggcgttcctg ggcatcagtg tgcaggtccg gcgcgtagaa gtgatgtcgc ccgcggccgc
     3841 cgtccttggc cgcatagagc gcgagatcgg cattgcggat gatcgcctcg ctggtcaccc
     3901 cgtcgtcggg cgcgatggcg atgccgaccg aggcgccgat catcacccgg ttgccgtcga
     3961 tctggtatgg ttcggccaag gccatgatga tccgctcggc gcggtgagcc aaggcgttgc
     4021 gggtgctgcg gccggggatg attacctgaa actcgtcacc gccgagtcgc cccacgcgcc
     4081 cggggctttc caccacccgc tcaagccgct gcgccacctg ccggagcagg gcatccccgg
     4141 caggatggcc gagggtgtca ttgacctgct tgaaccggtc gagatcgagc aggaacacgg
     4201 cgcaggtgcg gttttcgaca gcgcgggcgt tgagtatctt ttcgagcgat ttcgacatct
     4261 gcagccggtt ggcgaggcct gtgagcgaat cgtagtgcgc gaggcgtgtc gcctgttcct
     4321 cgtggcgctt gcgctcggta aggtcgctgc cggaaccgcg gaagccttgg aaattgtcga
     4381 aatcgtcgta aaccggcagg ccgttgaccg accaccagcg ctcctcgccg tcgagggcgg
     4441 cagcccgcag gcgcaagtcc tggaaagagg agcgggcgtt gaggtggaag tgcagcgtgc
     4501 gctcgctttc gccgctttgg ccttcttcaa ggaacaggtc ggtgaagggg cggcccacca
     4561 gctcgccctc gtccgcaccg acgatctcgc cgatcgtgcg ggagacatag gtgatcagcc
     4621 cgcgccggtc ggtttcccag aaccagccct ggcgggtcga ttcgtaatcc gagaggatca
     4681 gctcggcgcg ctgctgggcg cgctcgacct gttcgagccg gtcctgcgcc atgcggttga
     4741 gttcgatctg gcggaaggaa atatatacgc cgctgcccgc cccgatcacc agtgccagca
     4801 tcgtgcccgg ctcgaggctc accagcacat gggcaaacca cagtgagagc agtgccgcga
     4861 ccatggttgg caggcggccg gagcagagca cgccgaccag catgccgatc atgaccagcg
     4921 ccgccggccc ggtctgccag ccggcaccct gcgcgaggat cgaccattgc gacatggcga
     4981 ggccgaacag gaacatcggc gcgatcacca cggcgccgat cgcggcccag cgctgcaccg
     5041 gaccgcagtc gtgcccgcgc tcgtagcgca cggccagcgg cgcggcaaag gcgagcaggg
     5101 ccgccacgat tgccgcgact ccgctcggca ccgggggcag cgaatgcgag gcccacgcgt
     5161 ggatgaccac ggcgaccagc atgatcggcg ccatcatcca ccactccagc ggaatggcat
     5221 agcgcgccac gaatcgtcgc ggccgcaggc gcgccgtgct ggcccgcccg cccgcccggc
     5281 ccggcgccag caccggcgcc ggacggctgt ctggctcggg cgcttcgggg aatgacaggg
     5341 ccggtttgcg gagcatcgcc ccatctgctc cggtggggct tgcaaaagcg ttaaaccgat
     5401 tgtccccgca gcgaagcgat tcccccttgc gccgcgcgcc ttttggcggt tgcctgcggt
     5461 aaatggcccg taaggggcag attcgggaga cgaaccttgc ggcacctggc gatcatcggc
     5521 tccgggccgg ccggctatta cacggcggaa gccgcgctca agcggtgggg cgaggatgtc
     5581 caggtcgacg tgttcgaccg gctgccggtg ccttatggcc tgatccgcag cggggttgcc
     5641 cccgatcacc agtcgatcaa ggcggtgagc aagcgctacg agggtgtggc gctatcgcag
     5701 aacgtccggt tcatcggcaa tgtcacggtg ggtacggaca tcaccatcgg cgagctgcag
     5761 ggcctctatg acgcggtgat cctcgccacg ggggcgccga acgaccggcc cttggacctg
     5821 ccgggcgaac atctgggcaa tgtcttcggc agcgccgcct ttgtcggctg gtacaatggg
     5881 cacccgcagt tcgccgattt gaatcccgac ctttccggt
//
LOCUS       DATNBV010000510         3042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4117088,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000510 DATNBV010000000
VERSION     DATNBV010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4117088"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VN627_12085"
     CDS             <1..536
                     /locus_tag="VN627_12085"
                     /inference="COORDINATES: protein motif:HMM:NF019174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HXD04035.1"
                     /translation="RRSASHAGPSGDQRGNADFVRRLEERISCLAANQDLLVRRAWSN
                     VPLGELVEVQLAFLGEALDQVERVGPDLPVTPGAAETIGMALHELATNALKYGALSVP
                     GGKVRIDWMRAPDGGLAMGWSEHGGPPVTPPASQGFGNRLIADMPRGKLRGEVEVDYA
                     RDGFRWQLVCPAEKVLA"
     gene            690..1088
                     /gene="apaG"
                     /locus_tag="VN627_12090"
     CDS             690..1088
                     /gene="apaG"
                     /locus_tag="VN627_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HXD04036.1"
                     /translation="MKELFQHAAMTGGVTVRVAVNFLPEQSRIEAGKWFWVYHIRIEN
                     DSEQAVQLLTRHWRITDGAGHVSLVDGDGVVGEQPVIAPGASHDYVSGCPLSTPQGSM
                     EGHYSLRRADGTRFEAAIPYFPLAAPATAG"
     gene            1127..1912
                     /locus_tag="VN627_12095"
     CDS             1127..1912
                     /locus_tag="VN627_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXD04037.1"
                     /translation="MKRTHLPLNALRVFDAAARHLSFTRAADELAVTPAAVGQQIRAL
                     EDLLGVVLFRRTSKGLELTEEAAAGLEPLRTGFLHFEDAVRAMQAGQASHVFTIAAPR
                     DVLATWLAPRLAAYRADNPDVRYALVDAENADFTEANLDLAIRWTEGPGDLEGVPLGA
                     ARVVIVGAGPWIAWPGDPHPGGEGAEESPVHVADAGLALSAATAGLGRARVPQLLAEA
                     WHEAGRLPDLGEAQDCRRGYWLVAPLPQWRQKKVRALVAALTA"
     gene            complement(1918..2064)
                     /locus_tag="VN627_12100"
     CDS             complement(1918..2064)
                     /locus_tag="VN627_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HXD04038.1"
                     /translation="MLLTIALILFVLWLLGVVVFHVSGAIIHVLLVLAIIVGLVQLFR
                     GRRV"
     gene            2470..>3042
                     /locus_tag="VN627_12105"
     CDS             2470..>3042
                     /locus_tag="VN627_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXD04039.1"
                     /translation="MTARRLNREDSREVTRARLRRSAIRAFARHGVAGARIEAIAEDA
                     GYSRGAFYSNYKTKLDLLIDLLREKQIGEIRLWHEVLGHSGDVDQALASLSARYDAGS
                     DVHERALLNAELQLEAERNPEFRPVYEAYLDAVYAEMRELFITLFKRLGKQVSGNLDA
                     VLVTTRLLGLGLGAPSTLGRAIAARETPGRI"
BASE COUNT          502 a          948 c         1045 g          547 t
ORIGIN      
        1 cccgccgttc ggccagccac gctgggccgt caggggacca gcgcggcaat gctgatttcg
       61 tgcggaggct ggaagaacgc atctcgtgcc tcgccgccaa ccaggatctg ctggtacgcc
      121 gcgcctggtc caatgtcccg cttggtgagt tggtcgaggt ccagctggca ttcctcggcg
      181 aggcgctgga tcaggtcgag cgggtggggc cggacctgcc ggtaaccccg ggcgctgccg
      241 agacgatcgg catggccttg catgaactgg caaccaatgc gctgaaatat ggcgccctgt
      301 cggttccagg cggaaaggtg cggatcgatt ggatgcgggc cccggatggc ggcctcgcca
      361 tgggctggtc cgaacatggc ggccctccgg tgaccccgcc cgccagccag ggtttcggca
      421 accgcctcat cgccgatatg ccgcggggca agctgcgcgg ggaggttgag gtggactacg
      481 cgcgtgacgg cttccgatgg cagctggttt gcccggcgga gaaggtgctg gcgtaattgt
      541 tgggtgcgcc ttcgacatcc gtcgaaggtt tgcggtccct caagaaggcg cggaggcgtt
      601 gttgcgcacc atcaaaaagt ggaaaaaggg aacctgatct caccgtatgg ggttcacccc
      661 gtgcagggaa aggattaccc atggtgacca tgaaggaact cttccagcac gcggcgatga
      721 caggcggggt aaccgttcgc gtagccgtga acttccttcc cgagcaatcg cggatcgagg
      781 cgggcaagtg gttctgggtc tatcacatcc ggatcgagaa cgattccgag caggccgtcc
      841 agttgctcac ccgccactgg cggatcacag acggggcagg tcatgtctcg ctggtcgatg
      901 gcgatggcgt tgtgggcgag cagccggtga tcgcgccggg cgccagccac gactatgtct
      961 cgggctgccc gctctcgacg ccgcagggct cgatggaagg tcactactcg ctccgtcggg
     1021 ccgatggtac gcggttcgaa gcggcgatcc cgtacttccc gcttgcggct ccggccaccg
     1081 ccggttgaga gttgtcgcgg ggcctcggcc cgactagacc gctcgcatga agcgtaccca
     1141 cctgccgctt aacgccctgc gcgtgttcga tgccgcagcg cggcatctgt cattcacccg
     1201 cgccgcggac gaactggcgg tgaccccggc cgccgtcggc cagcagatcc gcgcgctgga
     1261 agacctgctc ggcgttgttc tgttccgccg cacgtccaag gggctggaac tgaccgagga
     1321 agccgcagcc gggctggagc cgctgcgcac cgggttcctc catttcgagg atgcggttcg
     1381 ggcgatgcag gccgggcagg ctagccacgt gtttaccatc gccgctccgc gtgacgtcct
     1441 cgccacctgg ctggcgccgc gccttgccgc ctatcgcgcc gacaatcccg atgtgcgcta
     1501 tgccctcgtc gatgcggaga acgccgattt caccgaggcc aacctcgacc tcgcgatccg
     1561 ctggaccgag ggacccggcg atcttgaagg cgtgcccttg ggcgccgcgc gggtggtgat
     1621 cgtgggtgcc gggccatgga tcgcctggcc cggcgatccg caccccggcg gcgagggtgc
     1681 cgaggaatcc ccggttcatg tcgccgacgc cggacttgcg ctaagcgccg caacggcggg
     1741 ccttggccgt gcgcgggtgc cgcagctgct ggctgaggcc tggcatgagg ccgggcggtt
     1801 gcccgacctc ggcgaggcgc aagattgccg gcgcggctat tggctggtcg cgcccctgcc
     1861 gcaatggcgg cagaagaagg tcagggcgct ggtcgcagcc ctgaccgcat aggctggcta
     1921 aactctcctg ccgcggaaca gctggaccag gccgacgatg atggcgagga cgagcagtac
     1981 gtgaatgatc gcgcccgaga cgtgaaacac cacgacgcct agcagccaca gcacgaacag
     2041 gatgagggca atggtgagaa gcatgggttg cgccctttcg gttgttgcgt tcaccgccag
     2101 aacggggctg ctcaccggaa agttccctcg cgattattac cgatgaatca tgtgcttgtt
     2161 ggcgcggacg gagtcatgat cgttgctcct cactcaggtt gaaggagcat gtccgatgat
     2221 cgagaccgga atggatcgcc gcccgggctt tcggagcggt tgctagtcgg cggcgctgga
     2281 ctggcgggct gcggatcggc ggttcggggc cgtcgcgggt tgagcgcagc gcaatcgcat
     2341 taaattgaat tcattgccgc gtacgcttcg gctgcctcaa ccacggacgt ggcggcggca
     2401 ggacttgcac gcttgacgtg atgcgccgtt gtattcaaat acgctcgtgt atctcgaatc
     2461 ggatgggcaa tgacggccag acgcctaaat cgtgaagaca gccgcgaggt tacccgggcc
     2521 cgcctgcgcc gatcggcgat ccgcgccttt gcccgtcatg gcgtcgcagg cgcacggatc
     2581 gaggctatcg cggaagatgc tggttattcg cgaggcgcgt tctattcgaa ctacaagacc
     2641 aagctcgacc tgctgatcga tctgctgcgc gagaagcaga tcggagagat ccggttgtgg
     2701 cacgaggttc tcggtcatag cggtgatgtc gaccaggcgc tcgccagcct gtcggcccgc
     2761 tatgatgccg gttcggatgt gcacgaacgt gccctgctca acgccgagct tcagctggag
     2821 gcggagcgca atcctgagtt ccgaccggtc tacgaggcct atctcgacgc tgtctacgcg
     2881 gaaatgcgcg aacttttcat caccttgttc aagcggttgg gcaagcaggt cagcggcaat
     2941 ctcgatgcgg tgctcgtcac cacccgcttg ctgggtctgg gcctgggcgc gcccagcaca
     3001 ctcgggcgcg ctatcgccgc gcgggagact cccgggcgga tc
//
LOCUS       DATNBV010000511         3573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4756153,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000511 DATNBV010000000
VERSION     DATNBV010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4756153"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VN627_12110"
     CDS             complement(<1..1216)
                     /locus_tag="VN627_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD04040.1"
                     /translation="MPSYKAPVRDARFVINEVLRIGDRNGLPGFSAATPDLVATVLEE
                     SGKFAAEVCAPLNLSGDQAGCQRRQDGSVTTPEGFKAAYDQFVASGWGTLSQPEAFGG
                     QGLPHVLGFAFEEFLSSANQSFAMYPGLTNGAVGAILTKGSPEQQAAYLPKMISGEWS
                     GTMNLTEPQCGTDLGLIQTKAEPRSDGIHAITGTKIFISAGEHDLTANIIHLVLARLP
                     DAPEGTRGISLFIVPKFLVNADGSLGARNAVSCGALEHKMGIHANATCVMNYDGATGY
                     LIGEPNKGLAAMFIMMNAARLGVGMQGLSQAEVAYQNAVTYALERRQGRALAGPAEPG
                     EKADLLFVHPDVRRMLMDAKAFTEGMRALSLWGGLLVDLVHAAPTEEERQEADDLLGL
                     LTPVIKGYGTDKG"
     gene            complement(1274..1645)
                     /gene="hisI"
                     /locus_tag="VN627_12115"
     CDS             complement(1274..1645)
                     /gene="hisI"
                     /locus_tag="VN627_12115"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HXD04041.1"
                     /translation="MSSDDALREQGSVFMPKFDAAGLLTAVAVDSASGKVLMVAFMDA
                     EALAATRATGKAHFHSRSRRRLWMKGETSGHVLEVKQILVDCDQDALVLQCEPAGPAC
                     HTGAPTCFYRLLEDDRLERVV"
     gene            complement(1993..2745)
                     /locus_tag="VN627_12120"
     CDS             complement(1993..2745)
                     /locus_tag="VN627_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HXD04042.1"
                     /translation="MQVPAPIAPFVDLFFPPRCPLCGAPLTQQVGLCGECWSGMAIPA
                     DPSCAKCQRPFGEGVPEGSICAPCLAAPPVHDGIAAATLYNDPARRLVLAFKRGGRVA
                     LAPFLGRLMAARLMHPGLPQVGPGWLIVPVPLHRWRLWTRGFNQSALLGATIARERGG
                     RLLVDGLVRRKATPVLGGLGAKARARALVGAIAVNPKRAAQLRGADVVLVDDVLTSGA
                     TTEACVKVLKRAGARRVVVACFARVLGEAMPH"
     gene            2825..>3573
                     /locus_tag="VN627_12125"
     CDS             2825..>3573
                     /locus_tag="VN627_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXD04043.1"
                     /translation="MSAAPPQIFAPERRAAIVRRAAALQRAADAPRYLAEDIAEDTLE
                     RLGFLRHQPGSALVLGDLSGAVAGALAAAGAQVTIPDPEMSLDQPWSAPGFDLIVAAL
                     ALDTVNDLPGALVHARQALAPGGLLLVTLLGAGSLPALRAIMLAADGDRPSPRLHPQV
                     DVRAGAQLLQRASLADPVADSRSLVVRFSSFSRLVGDLRAQGLSSALVQAGPPLTRVA
                     YRRALAAFADRAGPVGKGGKGTSIGHAGQWRA"
BASE COUNT          605 a         1255 c         1124 g          589 t
ORIGIN      
        1 agcccttgtc ggtgccgtat cccttgatca cgggggtgag cagaccaagc aggtcgtcgg
       61 cttcctgccg ctcctcctcg gtaggggctg cgtggacaag atcgaccagc agcccgcccc
      121 acagcgacag cgcgcgcatg ccctcggtga aggccttggc gtccatcaac atgcgccgca
      181 cgtcgggatg gacgaacagc aggtcggcct tctcgcccgg ttccgccggc ccggccagcg
      241 cccgaccctg gcgccgctcc agcgcatagg tgaccgcgtt ctggtaggcg acttcggcct
      301 gggacaggcc ctgcatgcca acgcccaggc gcgccgcgtt catcatgatg aacatggccg
      361 ccagcccctt gttcggttca ccaatgaggt atcccgtggc tccgtcgtag ttcatcacgc
      421 aggtggcatt ggcatggatg cccatcttgt gctccagcgc cccgcagctc accgcattgc
      481 gcgcgccaag cgaaccgtcg gcattcacca ggaacttggg cacgatgaac agcgagatgc
      541 cgcgcgttcc ttcgggcgca tcgggcagcc gggcgagaac gaggtggatg atgttggcgg
      601 tgaggtcatg ctcgcccgcg gagatgaaga tcttcgtgcc cgtaatggca tggattccat
      661 cggaccgagg ttcggccttt gtctggatca ggccaagatc ggtgccgcac tggggctcgg
      721 tcaggttcat cgtgcccgac cattcgcccg agatcatctt cgggagataa gccgcctgct
      781 gctccggcga acccttggtc aggatcgcgc cgaccgcgcc attagtcagc cccggataca
      841 tggcgaacga ctggttggcg ctggagagga actcttcgaa ggcgaagccg agcacatgcg
      901 gcaaaccctg cccgccaaac gcttccggct gcgacagcgt accccagcct gaggcaacga
      961 attggtcgta ggccgccttg aacccctccg gggtagtcac gctgccatcc tgacggcgct
     1021 gacaccccgc ctgatcgccg gaaaggttca gcggcgcgca aacctcggcg gcgaactttc
     1081 cgctttcctc cagcacggtg gcaaccaggt caggcgtggc ggcactgaaa ccgggcagtc
     1141 cattgcgatc accgatccgc agcacttcgt tgatgacgaa ccgcgcgtcg cgaaccgggg
     1201 ccttgtagct gggcatggac cactccctcc agaaagtgat cccaaggtcg ggattgatgg
     1261 cctcaacgtc aactcaaacc acgcgttcga gccggtcgtc ctccagcagg cgatagaaac
     1321 aggtcggtgc gccagtgtgg caggccggcc cggctggttc gcactgcaga accagcgcat
     1381 cctgatcgca gtcgaccagg atctgcttca cttccagcac gtggcccgac gtctccccct
     1441 tcatccacag ccgcctgcgg ctgcgcgagt ggaagtgggc cttgccggtg gcccgcgtcg
     1501 ccgccagcgc ctcggcatcc atgaaagcca ccatcagcac cttgcccgaa gccgaatcga
     1561 ctgccaccgc ggtcagcaat cctgcagcat cgaacttggg catgaacacg ctgccctgct
     1621 cgcgcagggc gtcatcgctg gacattgcag gaaattccct attcggaccc tgcggattgc
     1681 aggggtttgt tgcacgataa ccacagccgg tcaccaaaat ccacgccccg cgcggatcgc
     1741 tcttttcttt cctgtcgatt ccatgaacag aggctgtgcg gttcagagat gaactgcccg
     1801 caaccagcgg cgccaggcaa gagcgccagg gttcagggtg aatatcggca gtgccagcca
     1861 tgcagcgagg cacgccggat tgagggatcg ggtggcgatc gtgggggcga tcgcttaaac
     1921 tgcgcaagca gagcccaaca gtttcgtcac gagggcgccc ggaatttcgg ttccgggcgt
     1981 ttccttttgc agctaatgcg gcatggcctc ccccaaaacc cgggcaaaac aggccacgac
     2041 cacgcgtcgg gctccggctc gcttcagcac cttgacgcag gcctcggtgg tcgcgccgct
     2101 ggtcagcaca tcgtccacca gcaccacgtc tgcgccgcgc aattgggcag cgcgcttggg
     2161 attgacagca atggccccga ccagcgcccg agcgcgcgcc ttggcgccca gcccgcccag
     2221 gaccggagtg gccttgcgcc gcaccagacc atcgaccaag agccgccccc cgcgttcgcg
     2281 agcaatcgtg gcgccgagca gggccgactg gttgaacccc cgcgtccaca gccgccagcg
     2341 gtgcagcggc acgggcacga tcagccatcc cggcccgacc tgcggcagcc ccggatgcat
     2401 cagccgggcc gccatcagcc ggccaaggaa cggggccagc gccacgcgtc caccgcgctt
     2461 gaaagccagc accagtcgcc gggccggatc gttgtagagc gtcgccgccg cgatcccgtc
     2521 atgaaccggc ggtgcggcca ggcatggcgc gcagattgag ccttcgggca ctccttcgcc
     2581 gaatgggcgc tggcacttgg cgcaggaggg atccgccgga attgccattc cgctccagca
     2641 ctcgccgcac agcccaacct gctgcgtgag cggtgccccg cacagcgggc agcgtggcgg
     2701 gaagaacagg tcaacgaagg gagcaatcgg cgccggaact tgcatgaccg ccaacatacg
     2761 attgcagaca ctccccgcaa gagagcgctt gcccctgacc cccgccgcgg cctaaccggc
     2821 gcgaatgtcc gccgcaccac cccagatttt tgccccagag cgccgggccg ccatcgttcg
     2881 ccgcgcggcc gcactccaaa gggccgcaga tgcgccgcgc taccttgccg aagatattgc
     2941 cgaggacacc ctcgaacgcc ttggcttcct gcgccaccag cctgggtcgg ccctggtgct
     3001 gggtgatctt tccggggcag tagccggcgc tttggcggcg gcgggggcgc aagttaccat
     3061 tcccgatccg gagatgtcgc tcgatcagcc ctggtccgct ccagggtttg acctgatcgt
     3121 tgccgccctc gccctcgaca cggtcaacga tctgccgggc gctctggtcc acgcgcgcca
     3181 ggctcttgcg cccggtggcc tgctgctggt gaccctgctc ggtgcgggta gccttccagc
     3241 cctgcgtgcg atcatgctcg ccgccgatgg agatcggccc tcgccgcggc ttcatccgca
     3301 agtcgacgtc cgtgccggcg cgcaactgct tcagcgtgca agtctggccg atccggtggc
     3361 cgacagccgg agcctcgtcg tccgtttttc cagcttctcg cggctcgtgg gcgacctgcg
     3421 tgctcagggc ctctcctcag cgctggtcca ggcaggcccg ccactgaccc gcgtggccta
     3481 tcgacgtgcc cttgccgcct ttgccgaccg ggccggcccg gtcggcaaag gcggcaaggg
     3541 cacgtcgata ggccacgcgg gtcagtggcg ggc
//
LOCUS       DATNBV010000512         3979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_592042,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000512 DATNBV010000000
VERSION     DATNBV010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592042"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VN627_12130"
     CDS             <1..1019
                     /locus_tag="VN627_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD04044.1"
                     /translation="GLLLAWQHLAVTTVAIPLMLAALFAMGVHSTFFGPIKYAILPQH
                     LHKDEVLAGTGLVEAGTYVAILAGTILAGWIPVEWAAAGVIVTALAGWFTARFVPPAP
                     PQGEVVPLDHHFIRASIRLIRQTAHDRRVFLAIGAISFFWAVGTILFIQFPPLAKNVL
                     MASKEVASLFLVVFSIGVALGSVSINALLKGRVSARYAPAAVLAMSLFVVAFWAVCSR
                     WTADPAAGLMNAAEFVRHPLAVELLLSLLGIAVAGGMFVVPLYAFLTTFVDKAQTART
                     IAANNIVNSGAMVGGALLAMGLSAAGVPVVSQLLLSAGMGLVCAWLGWRLFRAECRHC
                     PAAE"
     gene            complement(1023..1259)
                     /locus_tag="VN627_12135"
     CDS             complement(1023..1259)
                     /locus_tag="VN627_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04045.1"
                     /translation="MSTAIFGTARHPFRATLPLRTLRRLPRHEPEVQARIDGCRGEVL
                     HQPEQRRAWLSAQDLRDFLIAYIACFMAVSAFLS"
     gene            complement(1416..2324)
                     /locus_tag="VN627_12140"
     CDS             complement(1416..2324)
                     /locus_tag="VN627_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HXD04046.1"
                     /translation="MQTSDMTAKMAAGQGFIAALDQSGGSTPKALAGYGVTDDAWSTE
                     EEMFGLIHEMRCRIIRSSAFTGEKVIGAILFERTMDGTVDGKGVPAALIEKGVVPFIK
                     VDKGLEDEANGVQLMKPMPELDALLTKAKALGVYGTKERSVINSANEAGIAAVVKQQF
                     DVGKQVLAHGMMPIIEPEVNIKSETRKECDAILLKEILKNLDELPEGQQVMLKLSLPV
                     VAGTFDPLVKHPKVLRVVALSGGYKRPEACIELAKNAGIIASFSRALLEDLRHQMSDA
                     EFDASLAGAIDEIHAGSTQKTAPVAA"
     gene            complement(2442..2732)
                     /locus_tag="VN627_12145"
     CDS             complement(2442..2732)
                     /locus_tag="VN627_12145"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HXD04047.1"
                     /translation="MPIPRLSDAEIAQLVTELPGWTLRADGLAIERAFRFGDFSEAFG
                     FMTRVALLAEQHDHHPEWSNVYNRVAITLTTHDCGGLSARDATMARAIARLV"
     gene            2781..3359
                     /gene="ccmA"
                     /locus_tag="VN627_12150"
     CDS             2781..3359
                     /gene="ccmA"
                     /locus_tag="VN627_12150"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HXD04048.1"
                     /translation="MQRGHIHARDLACRRGERILFARLSLELRAGEALHVTGPNGTGK
                     SSLIRILAGLLPAFAGEVAIDGAAALLDERPALDPHLPLARALAFWERIDGHAADAVG
                     RLGLGELGDVPVRFLSTGQKKRAAMARLIAQGAPVWLLDEPLNGLDTAAVALIEQLVD
                     EHCGAGGLAVVASHQPIALPGAARLVITDYAA"
     gene            3356..>3979
                     /locus_tag="VN627_12155"
     CDS             3356..>3979
                     /locus_tag="VN627_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HXD04049.1"
                     /translation="MSRQMLALLRRDLALLLFGGRRGGAMLPLVFFLAVAMLFPFGVG
                     PDPRLLARTGGGVVWIAALLAAILPLDRLIEPDVELGMFDQWALRGISEEWIVAIRVL
                     AHWLSFGPLLMVAALPAAALLGLDGATVRTLELGLLAGTPGLAAVGVMVAALTAGLRG
                     GAALAGLLVIPLAVPLLVFGAGSLAPGGETGVALTAAVSLVLLAIAPI"
BASE COUNT          558 a         1440 c         1270 g          711 t
ORIGIN      
        1 cgggactgct cctcgcctgg cagcacctgg cggtaaccac cgtcgccatc ccgctgatgc
       61 tcgccgcact cttcgcgatg ggggtccact ccaccttctt cggcccgatc aaatacgcga
      121 tcctgccgca gcacctccac aaggacgaag tgctcgccgg caccgggctg gtcgaggccg
      181 gaacctatgt cgccattctc gcggggacga tcctggccgg ctggattccg gtggaatggg
      241 ccgcggcggg ggtcatcgtc accgctctcg ccggctggtt caccgccagg ttcgtaccgc
      301 ctgctccgcc gcagggcgag gtggtcccgc tcgatcatca cttcatccgc gcctcgatcc
      361 gcctgatccg gcaaaccgcg cacgatcgcc gggtcttcct cgccatcggt gcgatcagct
      421 tcttctgggc cgtcggcacg atcctgttca tccagttccc cccgctcgcc aagaacgtcc
      481 tcatggccag caaggaagtg gccagcctgt tcctggtggt cttctcgatc ggcgtggccc
      541 tgggttcggt ctcgatcaac gccctgctca agggccgcgt ctcggcccgc tatgccccgg
      601 ccgcggtgct ggcgatgagc ctgttcgtgg tcgccttctg ggcggtctgc tcgcggtgga
      661 ccgccgatcc cgccgccggc ctcatgaacg cggccgagtt cgtccgccac ccgctggcgg
      721 tcgaactgct gctgtcgctg ctggggatcg cggtggcggg cggcatgttc gtcgtcccgc
      781 tctacgcctt cctcaccacc ttcgtcgaca aggcccagac cgcgcggacg atcgcggcca
      841 acaacatcgt caattccgga gccatggtcg gcggggccct gctggccatg gggctgagcg
      901 cggccggcgt gccggtcgtc agccagctgc tgctcagcgc ggggatgggc ctggtctgcg
      961 cctggcttgg ctggcggctg ttccgggccg aatgccggca ctgcccggca gcggaataga
     1021 cctcagctga gaaacgcgct caccgccatg aagcaggcga tgtaggcaat aaggaagtcc
     1081 ctcaagtcct gggccgacag ccatgcccgg cgctgctccg gctggtgcaa cacctcgccg
     1141 cgacagccat cgatccgcgc ctgcacttcc ggctcatggc gcggcaggcg gcgcagggtg
     1201 cgcagcggca gggtggcgcg gaacgggtga cgggcggtgc cgaagatcgc ggtgctcata
     1261 ccgcaaagaa ttaacggcgc gtttacttag ttccagagcc cccgccccgg catttccccg
     1321 ccatcccgtt tcggaacaat ccgccgcccc gccgccaacg acagcgccgg acaggttgcc
     1381 ctgcccggcg ccgaagtttt cacatgaaat cagcttcagg cggcgacggg cgccgtcttc
     1441 tgggtcgaac cggcgtggat ctcgtcgatc gccccggcca gcgaagcgtc aaattcggca
     1501 tcgctcatct ggtggcgcag gtcttccagc agggcgcggc tgaagctggc gataatcccg
     1561 gcgttcttgg ccagctcgat gcaggcttca gggcgcttgt agccgcccga caacgccacg
     1621 actcgcagga ccttgggatg cttgaccagc ggatcgaagg tgccagccac caccggcagc
     1681 gacagcttga gcatgacctg ctggccttcg ggcagttcat ccaggttctt gaggatttcc
     1741 ttgagcagga tcgcatcgca ttccttgcgc gtctcgctct tgatgttcac ctcgggctcg
     1801 atgatcggca tcatgccatg ggcgaggacc tgcttgccca cgtcgaactg ctgcttgacc
     1861 actgcggcga tcccggcctc gttggctgaa ttgatgaccg aacgctcctt ggtgccatag
     1921 acgcccaggg ccttggcctt ggtgagcagc gcatcgagct caggcatcgg cttcatcagc
     1981 tggacgccgt tggcctcgtc ttccaggccc ttgtcgacct tgatgaaggg caccacgccc
     2041 ttctcgatca gcgcggcggg aacgcccttg ccgtcaaccg tgccgtccat cgtccgctcg
     2101 aacaggatcg cgccaatgac cttctcgccg gtgaaggcgg acgagcggat gatccggcag
     2161 cgcatttcgt ggatcaggcc gaacatctct tcttcggtcg accaggcatc gtccgtgacg
     2221 ccatagccgg ccagggcctt gggagtggag ccgcccgact ggtcaagcgc ggcgatgaaa
     2281 ccctggccgg cggccatctt ggcggtcatg tcggacgtct gcatgaagtt cttgcccttt
     2341 tccctgatga tagtgcgtgc ccaacgcgcc agtggcgatt ctgccgcgcg catacgtatg
     2401 ctgggctcca tagcaagatt cgcgcgcggg gcaaccggcg gctaaaccaa ccttgcgatc
     2461 gcccgcgcca tcgtcgcatc ccgcgccgag agaccgccgc aatcatgggt ggtgagcgtg
     2521 atcgccaccc gattgtagac gttgctccat tccgggtgat ggtcgtgctg ctcggccagc
     2581 aaggcaaccc gtgtcatgaa gccgaaggcc tcgctgaaat cgccaaaccg gaaagcccgc
     2641 tcgatcgcca ggccatccgc ccgcagggtc catcccggca gctccgtcac cagctgcgca
     2701 atttcggcat cggacaggcg ggggatgggc atgacgggct ccttggcaat gcccggcagg
     2761 ttcgcctaaa gccaccagcg atgcaacggg gccacatcca cgcgcgcgac cttgcctgcc
     2821 gccggggcga gcggatcctg ttcgcccggc tcagcctcga actgcgcgcc ggcgaggccc
     2881 tccatgtcac cgggcccaac ggcaccggca aatccagcct gatccgcatc ctggccgggc
     2941 tgctgcccgc ctttgccggc gaggtcgcca ttgacggcgc ggccgccctg ctcgacgaac
     3001 gccccgcgct cgacccgcac ctccccctcg cgcgcgcgct cgccttctgg gagcgcatcg
     3061 acggccacgc cgccgatgcc gtcggccgcc tcggcctcgg cgagcttggc gacgtgccgg
     3121 tccgcttcct ctccaccggc cagaagaaac gcgccgccat ggcccgcctg atcgcccaag
     3181 gcgcacccgt gtggctgctc gacgaaccgc tcaacgggct cgacaccgcc gccgtcgccc
     3241 tgatcgaaca gctcgtggac gaacattgcg gtgccggcgg gctcgccgtg gtcgcctcgc
     3301 accagccgat cgccctgccc ggcgccgcgc ggctcgtcat tacggactat gcagcgtgag
     3361 ccggcagatg ctcgcccttc tgcgccgcga tctggcgctc ctgctgttcg gcggacggcg
     3421 cggcggggcc atgctgccgc tggtgttctt cctcgccgtc gccatgctgt tccccttcgg
     3481 cgtcggcccc gatccccggc tgctcgcccg caccggcggc ggggttgtct ggatcgccgc
     3541 gctcctcgcc gcgatcctcc cgctcgaccg gttgatcgaa cccgatgtcg aactcggcat
     3601 gttcgaccag tgggccctgc gcggcatttc cgaggaatgg atcgtcgcga tccgcgtact
     3661 cgcccactgg ctgagcttcg gcccgctcct catggtcgcc gcccttcccg ccgccgccct
     3721 gctcgggctt gacggggcaa ccgtgcgcac cctcgaactg ggcctgctgg ccggaacccc
     3781 gggccttgcc gccgttgggg tcatggtcgc cgcgctgact gccggcctgc gcggcggcgc
     3841 ggcgcttgct ggtcttctgg tgatcccgct ggccgtgccc ctgctggtat tcggcgcggg
     3901 cagcctggca cccggcggcg agaccggcgt ggcgctgacg gcggcggtaa gcctcgtcct
     3961 gctggccatt gcgcccatc
//
LOCUS       DATNBV010000513         3304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2490147,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000513 DATNBV010000000
VERSION     DATNBV010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490147"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            404..940
                     /locus_tag="VN627_12160"
     CDS             404..940
                     /locus_tag="VN627_12160"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXD04050.1"
                     /translation="MSAAPAPAPVAPAQPPAPAAARAPAPVHSAPPAPQPKARARSLA
                     DAFADLAVVPKGTVAPAPGAVDVRKLAEARARAEAAKTPPKPAHPSRIWVQVGTGRDT
                     GALGFTWRALVKDNPALFKGQSASISDWGRTNRLLAGPFATEAAAESFLAKAKKADLD
                     AFVWTSPAGQVVDALPSR"
     gene            1135..1620
                     /locus_tag="VN627_12165"
     CDS             1135..1620
                     /locus_tag="VN627_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HXD04051.1"
                     /translation="MERDDDAAPVEMLASLFEARGWQFEFVGDDEISGEVQGSWASYQ
                     LRGIWRREDHVLQLLCLPDIRIPDDKRAPMFEVLAMVNEQMWLGHFDVWSNGMVLLYR
                     HALMLGDEGLLSIAQAQTAVEAAIDECDRFYPAFQFILWGDKSPAEALASALVDAAGE
                     A"
     gene            1655..2434
                     /locus_tag="VN627_12170"
     CDS             1655..2434
                     /locus_tag="VN627_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HXD04052.1"
                     /translation="MGGSMLTGWLAGGREAGEFTVVDPFLKQAPDGVTLLREIPPGPP
                     FDVVLLGFKPQQIAEAADPVVGAIGPQTVVLSILAGVELATLRARFPQAGAIVRVMPN
                     LAAALGKSPIGLFGDGDGDGDTGARAVVDSLMAPLGTAEWLAGEDLMDVVTALAGSGP
                     AFVYRLIDALAAGAAALGLPRDQADRLALGMVEGAGALAAASPFAPGELADKVASPGG
                     TTRAGLDVLDADGRLAALMADTLRAARDRGAEMAAEARARS"
     gene            2558..3295
                     /locus_tag="VN627_12175"
     CDS             2558..3295
                     /locus_tag="VN627_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HXD04053.1"
                     /translation="MANWNDPQPRAGFGAASGLTAGAGRVSYDMGLRRYMLSIYNYMA
                     SGVLLSGIVALLFASSGAAAAVFATPLRWLIALAPLGFVMAMSFGLNRMQTSTLRMLF
                     WAFSVAMGLSLSSIFFVYTGPSIASTFFATAAAFAGLSLVGYTTKKDLSGFGTFLIMG
                     VWGLLAAMVINMIWPSQGLGLAISLLGVVIFAGLTAYDTQRLKMMYQQVAGTDFSGKM
                     IVMGALSLYLDFINMFTFLLQFMGDRR"
BASE COUNT          439 a         1173 c         1104 g          588 t
ORIGIN      
        1 gcctagcggt gaaccgctgg gtcgcggccg ctcgcgccag cccgagccgc gccagcctgt
       61 ccgcaccgcc gtcgcaccca cgccgccacc tgtgcgtacc gctcccccgg agatcgcgcc
      121 gacccgctcc gacctgccgg cgcagcgtcc cggcggcatg atggcggttg ctgcagccgt
      181 tccgccgccg gctcccgcgc ccgtcccggc gcctgcgcga gctgccgttc cggcgcagcc
      241 gccggtcgct gccgtggcgc cggcagtcgc tcccgtggtc gcccgggcgc cgcaatcgac
      301 cgtcgctgcc gcccctgctg tcctgccgtc gtcgtccccg acgcggacgg ctgcgctcac
      361 cggtcccggc ttgacctcag gcgccgggtt tgatcttggg cggatgtccg ccgcgcccgc
      421 tccggcccct gtcgcaccgg cgcaaccacc ggcgccggcc gctgcccgag ccccggcacc
      481 ggttcattcc gcgccgcccg cgccccagcc caaggcccgg gcgcgcagcc tggcagacgc
      541 cttcgccgat cttgccgtcg taccgaaggg cacggttgct ccggctcccg gtgcggtcga
      601 tgtgcgcaag ctggccgagg cccgcgcccg cgccgaggcc gccaagactc cgcccaagcc
      661 tgcccacccc agccgcatct gggtccaggt cggcacgggc cgcgatacgg gggcgctcgg
      721 cttcacctgg cgcgcgctgg tcaaggacaa ccctgcgctg ttcaagggcc agtcggcctc
      781 gatctcggat tgggggcgta ccaatcgcct gctcgccggg cccttcgcaa ccgaagccgc
      841 tgccgaatcc ttcctggcca aagcgaagaa ggccgatctc gacgctttcg tgtggaccag
      901 cccggcggga caggtcgtcg acgcgctgcc gtcgcggtga tgaagtgttg ctcacaggga
      961 acccctgtct tttccacacc ttgtaaacag cgttgtcgag ttttgcgcag gtggtagtta
     1021 accgcgcatt gtgcaaccgg cgccgcccga agcatccagc gctcgcaagg ccacggttcc
     1081 cgatgaccgg acggcgcgcg gacaggagat gatgtgagga ccagccagaa cgacatggaa
     1141 cgcgacgacg atgccgcgcc ggtcgaaatg ctcgcctccc tgttcgaggc gcgtggctgg
     1201 cagttcgagt tcgtcggcga cgacgagatc agcggtgagg ttcagggctc ctgggcgagc
     1261 taccagctgc gcggcatctg gcgccgcgag gaccacgttc tccagctgct gtgcctcccc
     1321 gacatccgta tccccgacga caagcgcgcc ccgatgttcg aggtgctggc catggtcaac
     1381 gagcagatgt ggctcgggca tttcgacgta tggtcgaacg gcatggtcct gctttatcgt
     1441 cacgcgctga tgctgggcga cgaggggctg ctctcgatcg cccaggcaca gacggcggtg
     1501 gaagcggcca tcgacgaatg tgaccggttc tatccggcct tccagttcat cctgtggggc
     1561 gacaagtcgc cggccgaggc cctcgccagc gcgctggtcg acgctgccgg ggaagcctga
     1621 cgtggcgcgc atcctgctgg tcggctgcgg caacatggga ggctccatgc tcaccggctg
     1681 gctcgccggt gggcgcgagg ccggcgaatt caccgtcgtc gatcccttcc tcaagcaggc
     1741 gcctgatggg gtgactctgc ttcgcgagat tccgccaggc ccgccattcg acgtcgtcct
     1801 gctcgggttc aagccgcagc agatcgccga ggctgccgac cccgtggtgg gtgcaatcgg
     1861 gccgcagact gtggtgctct cgatcctcgc cggggttgaa ctggcgacgc tgcgcgcccg
     1921 tttcccgcag gcgggtgcaa tcgtccgggt catgcccaac cttgccgcag cgctgggcaa
     1981 gtccccaatc ggcctgttcg gcgacggcga cggcgacggc gacactggcg cgcgggcggt
     2041 agtggatagc ctgatggccc cgctcggaac ggccgaatgg ctggcgggcg aggacctgat
     2101 ggacgtggtc accgcactgg ccggttcggg gcccgccttc gtttaccggc tgatcgatgc
     2161 tttggctgcc ggagccgccg cgctgggctt gccgcgcgac caggccgacc gcctggccct
     2221 tggcatggtc gaaggggcag gggccttggc cgccgcgtcg ccgtttgctc cgggcgagct
     2281 cgctgacaag gtcgccagcc cgggcggcac cacccgcgcc gggctcgacg tgctcgacgc
     2341 tgatggccgc ctcgccgcgc tgatggccga tacgctgcgg gccgcgcgcg atcgcggcgc
     2401 cgagatggcc gccgaggccc gcgccagaag ctgagctttt ccgcgcctgt tacactttgc
     2461 gacaggcgtc cttgtccggt tattcttgaa tccttcaccg atgagggcga tattcggccc
     2521 acggccctat cccgggcctc aatggagctt ggacacaatg gcaaactgga acgaccccca
     2581 gccccgcgcg ggcttcggtg cggcgtcggg ccttacggct ggtgccggcc gcgtgtcgta
     2641 cgacatgggc ctgcgccgct acatgctgtc gatctacaac tacatggctt cgggcgtgct
     2701 gctctcgggc atcgtcgccc tgctgttcgc ctcctcgggc gccgccgccg ccgtctttgc
     2761 gacgccgctg cgctggctga tcgcgctggc cccgctcggc ttcgtcatgg cgatgagctt
     2821 cggcctcaac cgcatgcaga ccagcacgct tcggatgctg ttctgggcat tcagcgtggc
     2881 catgggcctg tcgctgtcgt cgatcttctt tgtctacacc ggcccgtcga tcgccagcac
     2941 tttcttcgca acggctgcgg ccttcgcggg gctgagcctt gtcggctaca ccaccaagaa
     3001 ggatctgtcg ggcttcggca ccttcctgat catgggcgtg tggggccttc tggcggcgat
     3061 ggtcatcaac atgatctggc cctcgcaggg gctgggcctg gcgatttcgc tgctcggcgt
     3121 ggtgatcttc gcgggtctga ccgcttacga tacccagcgc ctgaagatga tgtaccagca
     3181 agtcgcgggc acggattttt ccggcaagat gatcgtgatg ggcgcgctga gcctttatct
     3241 cgacttcatc aacatgttca ccttcctgct tcagttcatg ggcgatcgcc gctgaaccgc
     3301 ggcg
//
LOCUS       DATNBV010000514         2555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2846664,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000514 DATNBV010000000
VERSION     DATNBV010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2846664"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1109)
                     /locus_tag="VN627_12180"
     CDS             complement(96..1109)
                     /locus_tag="VN627_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HXD04054.1"
                     /translation="MARLQPLQRTQTLIAVLTVVVLCAMAVESTGSKKVPHCCRAPAA
                     QVHGIALVPPANAAADIPAVAAEPNTFLPLAPADAEQINDARPLADRPLQPPLPTYMP
                     TAWDALARETARRCLATAVYYEAAGEGWRGELGVAQVVLNRVRHPAYPKTICGVVYQG
                     SNLPTGCQFTFTCDGRLARPPSPSGWKQAMAVAEAALSGTVEPRVGMSTHYHTRAVVP
                     YWAPSLDKLAVLGNHIFYTFAGAAGSRRAFSGQILAEEAPPGAIPLQANASQVDPAAT
                     VGLSAASPIPATRPSAVKADARIPTRLAADERGTLAERGGTLVQSDAVGNRLRSDDSL
                     RLR"
     gene            1190..2116
                     /gene="xrtV"
                     /locus_tag="VN627_12185"
     CDS             1190..2116
                     /gene="xrtV"
                     /locus_tag="VN627_12185"
                     /inference="COORDINATES: protein motif:HMM:NF035943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase V"
                     /protein_id="HXD04055.1"
                     /translation="MVSQPQTVAAADPGTPRRPVSALPVARLAASDLVLLAGCAAIAV
                     PTLVRVAQQGWSTEQGAHGPIVFISGLWLLWRAWPRAAPLATRGQGWLVALLVAVLLP
                     AWYLSRITAMIQLEGFLAYGLVLSAAYARLGAAALRQMLFPLFYLTFAFPFPDTVIDA
                     LTLPMKIGISHFSAWFLALFGYPVGGQGVMLQIGQYQLLVAAACAGLNSIVSLSALSL
                     LYIHLRYGDRLAYALLFAILIIPIAMLANFVRVLILLLLTYHFGESAAQGYMHTFAGL
                     VMFAVALIALGLADLLLQRFDRRATALRPVAA"
     gene            2113..>2555
                     /locus_tag="VN627_12190"
     CDS             2113..>2555
                     /locus_tag="VN627_12190"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HXD04056.1"
                     /translation="MISRRDTLLGGVYLAGAATAFARLPNRRRPQLARGEMERWVPNA
                     VGPWRYVSSQGVVLPTADELQDRLYDNIVSRTYTAPSAPPVMLMLAYNSEQDGVLQIH
                     RPEACYKAGGFALTPTRTGPVEIAPGRSLDANRFAASAPDRVEQVL"
BASE COUNT          356 a          855 c          898 g          446 t
ORIGIN      
        1 taaatccagc gaattcctgc gagccgtggc gtgacggcga tgaacggagc aaaacgccgg
       61 gaaccaaatg gccggggcac aaaatgggct ggcggtcatc gcaggcgcag gctgtcatct
      121 gagcgtagcc ggttacccac cgcatccgat tgcaccaggg tgccgcctcg ctcggcgagg
      181 gtgccgcgtt cgtccgcggc aaggcgggtg gggatgcggg catcggcctt taccgctgaa
      241 ggccgcgtgg ccgggatcgg gctggcggcg ctaagcccga ccgttgccgc aggatcaact
      301 tggcttgcgt tggcctgaag cggaatcgcg ccgggcggtg cttcctcggc aaggatctgg
      361 ccgctgaaag cgcgccggct gccggcggca ccggcgaaag tgtagaagat gtggttgccc
      421 aagacggcca gcttgtcgag cgaaggagcc cagtagggca caaccgcgcg ggtgtggtaa
      481 tgggtcgaca tgcccacgcg cggttcaacc gtgcccgaca gggccgcctc cgcgaccgcc
      541 atggcctgtt tccacccgga gggcgatggt ggccgcgcca ggcgaccgtc gcaggtgaag
      601 gtgaactggc agccggtcgg caggttcgat ccctggtaga ccacaccgca aatcgtcttg
      661 gggtaggccg gatggcgcac ccggttgagc acgacctggg ccacgcccag ttcgccgcgc
      721 cagccttccc cggcggcttc atagtatacg gccgtggcga ggcagcggcg ggccgtctcg
      781 cgggcgagcg cgtcccaggc ggtcggcata taggtcggga gcggcggctg gagcgggcgg
      841 tcggccagcg gccgggcatc gttgatctgc tcggcatcgg ccggggcaag cggcaggaag
      901 gtgttgggct cggctgccac ggcgggaatg tcggctgcgg cattggcggg cggtacgagg
      961 gcaattccat gaacctgggc tgccggcgcg cggcaacaat gtggaacctt tttcgatccc
     1021 gtcgattcaa ccgccatggc gcacagcacg actaccgtca ggacggcaat gagcgtctgc
     1081 gtgcgctgga ggggctgaag acgtgccaag ggtcatgttc tcgcaagtgt tcgcgtccgg
     1141 ccctaaacga gccggaagcc gatcgggtcc ccgcgggggc tgatcggcca tggtcagcca
     1201 accgcaaacc gtggccgccg cggatcccgg gacgccgcgt cgaccggtct cggctcttcc
     1261 ggtggcgcgg ctggccgcct cggatctcgt gctgctggcg ggttgcgcgg ccattgccgt
     1321 gcccaccctg gtgcgggtgg cccagcaggg ctggtccacc gagcagggcg cgcacgggcc
     1381 gatcgtgttc atctccgggc tgtggctgct gtggcgggcc tggccgcgcg ctgcgccgct
     1441 ggccacgcgg gggcagggct ggctggtcgc gttgctggtc gccgtgctgc tcccggcgtg
     1501 gtatctcagc cggatcaccg cgatgatcca gctcgagggc ttcctggctt atggcctggt
     1561 gctgagcgcc gcctatgccc gcctgggtgc ggcggcgctg cggcagatgc tgttcccgct
     1621 gttttacctt acttttgcct ttcccttccc ggatacggtg atcgacgcgc tgaccctgcc
     1681 gatgaagatc ggcatctcgc acttcagcgc ctggttcctc gccctgttcg gctacccggt
     1741 cggcgggcag ggggtgatgc tgcagatcgg ccaataccag ctgctggtcg ccgccgcctg
     1801 cgccgggctc aattcgatcg tctcgctgag tgccctgtcg ctgctctata ttcacctgcg
     1861 ctatggcgac cggctggcct atgccctcct gttcgcgatc ctgatcatcc cgatcgccat
     1921 gctggccaac ttcgtgcggg tgctgatcct gctcttgctg acctaccact tcggcgaatc
     1981 tgccgctcag ggctacatgc acaccttcgc cgggctggtg atgtttgccg tggcgctgat
     2041 tgcactgggc ctcgccgatc tcctgctgca acggttcgac cgccgcgcta ccgccctgcg
     2101 cccggtggcg gcatgatctc gcggcgcgat acgctgctcg gcggggtcta cctcgccgga
     2161 gccgccacgg cctttgcccg gctgccgaac cggcgccggc cgcaactggc gcgtggggag
     2221 atggagcgat gggtgcccaa tgccgtgggc ccgtggcgct atgtttccag ccagggcgtg
     2281 gtcctcccga ccgccgacga attgcaggac cggctctacg acaacatcgt cagccgcacc
     2341 tataccgcac cctccgcccc gccggtcatg ctgatgctgg cctacaacag cgagcaggac
     2401 ggcgtgctcc agatccaccg gccggaagcc tgttacaagg cgggcggctt cgcgctcacg
     2461 cccacccgca ccggtccggt cgagatcgcg cctggccgca gcctcgatgc caaccggttc
     2521 gccgcctcgg cccccgaccg ggtggagcag gtgct
//
LOCUS       DATNBV010000515         9537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2490927,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000515 DATNBV010000000
VERSION     DATNBV010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490927"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VN627_12195"
     CDS             <1..730
                     /locus_tag="VN627_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04057.1"
                     /translation="AAGKHGQGAMHAAAVGAGVVAGAAAFHGAAERGGRAPGGQFAVP
                     ADNGGQARVSFRGERPTFLGGCPPGLAKKYNGCNPPGLVKSSAVPWQPDWWGYPDSGY
                     RYFDGYLLRTSGPAVLGYVPLLGGPLFIGEIWPAAYETAPVPAYYSDYYDLGPDYRYY
                     DDTFYRLEPRTNRIVGVAGFLTGDEFVVGEPMPVGYDVYNVPWDYRDRYADGPDAWYR
                     YADGYVYRIDPKTMLILAAIQLLT"
     gene            742..1317
                     /locus_tag="VN627_12200"
     CDS             742..1317
                     /locus_tag="VN627_12200"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HXD04058.1"
                     /translation="MIKTLRAFVLGLALSGLAGGCQRHDASPEARASGGPGAAAPAQV
                     TLDAAIGKSAQFSALAEIIRQTGLAGVLGGKASYTVFAPTNAAFDTLGEPGRALRQPE
                     QRAALAALLREHIVPGMLTLGDLNQALERSSGKPLKLRSMGKGTLTVSREDGKIMVSG
                     ADGAKAALTNDSATARNGSALGIDGVLRKVA"
     gene            1526..2461
                     /locus_tag="VN627_12205"
     CDS             1526..2461
                     /locus_tag="VN627_12205"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD04059.1"
                     /translation="MRLDDRVAIVTGAGGGLGKAYADLLASRGAKVLVNDFGGSFDGM
                     GSDARYAEAAAAEIRAKGGVAEANGNSVATSAGAKAIVDDAMARWGRVDILVNNAGIV
                     SASTTLPTITDEQWEKDMAVSASGTFYMCRAVWQGMNERNYGRIVNIASGSWFGMGSG
                     LPYPAGKGAVWAMTRGLASTTNVSKNNVKANAIMPIAGSRMTALMGEEINALMQRDFP
                     ARAVAPVVALLAHDDAPCNGEMFSVGGGGFARVMVAVAAGYRGTDKDWTIEDAASHFS
                     EAMDVSRYTVPADALVDAELYQSDVPWDAFRAFIA"
     gene            complement(2539..4665)
                     /locus_tag="VN627_12210"
     CDS             complement(2539..4665)
                     /locus_tag="VN627_12210"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HXD04060.1"
                     /translation="MELETLTPADETVSPIALQVWRSNLEAIGNEGLYGIERTAISPV
                     VVEALDCATAILDHQGRLIAFGGLSTNPGFAAIAHVRSVIRHHGDTIAEGDVFLGNDP
                     YADASLHPNDVFVSEPVYVAGELVGWIASTAHLMDVGGMVFGSFAPHATECFQESVRV
                     PPVRLFRKGVEQTDLWTMFLNNVRISDLVEMDLRALVAGCHVSSEKLAGLARSLGEDG
                     RRFRAMSDAFYRQTREAVDQRVAALQDGVYCKTAYADWNNEFFKLPCRLTVAGKRMVF
                     DFTGASPQCDHFINSRPYLIQGGIATPLGNYLFNDIPACQAIFEAVEVVCPEGTIVNA
                     TAPAPCGACHYDVPGTAASAATVCLVQAIAASPADIACSQYVTAQQGNYASDAFTVWS
                     YGGDGFAVMDGTTTGGSPAARDRDGIDLVDQNVGQHGMVELIDVEVFEDWYPVLVTGK
                     WPGGGGGSEGKFRGGAGMHMIFQPHLIDRIYGVTMAKKEHLPNSGVAGGRPGGGSELW
                     IHRPDGSREAVDNHTMGVELRAGESFEFKSSSGGGYGDPLERDPQAVQSDLALGRITS
                     EAALNIYGVVIDSTGVDGPATERRRQSVRAERLARAEPARHPLALTDAEFLPSGAAEA
                     VIYPGIGRRGAIAYSMASGAPLAKAPHHWTDGCPVLRERHPTRSGTDVIYDTYLDPVT
                     GAALEVDARLDGDERSFTSLPRSWTG"
     gene            complement(4670..6745)
                     /locus_tag="VN627_12215"
     CDS             complement(4670..6745)
                     /locus_tag="VN627_12215"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXD04061.1"
                     /translation="MLENLCIGIDVGGTFTDAVLTGAGEVIRAKALTTHGDPGRGVIA
                     ACELVAERLGVALDDLLPNVKRFGLGTTVVTNQIASRAGRRIGLITTAGFENIVPFSK
                     LHWGSENGWLVSPPQIISRDRIIGVAERIDRRGEVITPLDETAVLAAATTLVESREVE
                     ALAVSFIWSVRNDSHEARAVRLIRERYPDLPVSSAVSLLPVVREYERTMFCLLNSYTS
                     GALAGIDWLSEELARRGLSAPLLLIHSGGGATSVEEARQVPVSLAESGPAAGVGAAAM
                     LAASCGVSEAITCDMGGTTFDFSVLSRGEPARRVRGDLMGIWTALRSVDVDSIGAGGG
                     SIATINAFGALQVGPRSAGSMPGPACYGRGGTEATVTDALLVLGLLDEDTFLGGTMRL
                     DREAAVGACAKLGEKLGLEPEAVALGIREVALAGMIAAVRGRLAKLGIDPRGKSIVSY
                     GGCGSLFTPEIAAAIGASEVLISRNASVLSATGAATLDVRRERPRSVNARMPVDTGVL
                     VNLLTELMTEIDGDLERDGIPVDEREYQLEGELRFNRQKWELAIPFRSLKIDDTVVAQ
                     LEADFREEYISRFGSGSLMPGARPDLSTVRAIGIGRTTKAAVATSRESVERSLGRSSA
                     ANGSRNILVDEARGRQPVKVVSYDQVGRREQLCGPALIDGGDTTVFVPSTCTAQLHDN
                     GTLIISVNG"
     gene            complement(7413..7550)
                     /locus_tag="VN627_12220"
     CDS             complement(7413..7550)
                     /locus_tag="VN627_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04062.1"
                     /translation="MIFTRSATFVTPRFSSVALEKALIEIGTLLTSSARRRAVTTMAS
                     G"
     gene            7504..>9537
                     /locus_tag="VN627_12225"
     CDS             7504..>9537
                     /locus_tag="VN627_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXD04063.1"
                     /translation="MEKRGVTNVADLVKITPGLNISAGAIKSNPFVTIRGQSRGVTGN
                     INPGVITYLNEVPLPTQGALIPTYDMDNMQVLKGPQGTLFGRNSIGGAVLIYTKAPSD
                     DFGGYVKGEMASFDFKQIEGAINVPVVGDKLVIRLAGQVSSGGGYTKAIGVTPYTVDP
                     VTLVATPGHIRPLKHNYDEYDTGSFRVSVRAEPTDWLKNVTVADYTKIRGANNTLFHS
                     FYPQGFGGNPPALYMQPPATITAFLSSIFGPDAGPAFAANMIAVTQCGTSLTCDYRLA
                     QQAGQASDRVQFTNVDPFDSVAIVKGISNTTTIRLGDKHTLKNIFGYRSFFSYNPVDN
                     EGSAIDVADIDAQISMEEYTDELQLSGSFLNDRLKYTLGGFYYKEQPRGLGGNFGTEY
                     NVFQGLSHAQVTDYITNKSKAIYGQFDYSLDDLINGLTVTAGLRQTWDTVGGCTASAT
                     YSPFGQVYYTGRNLNWISQADCQKGSGLTTANVPGAIIVSAQTLPEVEFKKMTYTFGA
                     NWQITPDAMVYVTHRRGYRAGSYNTPLFDPYLAGSQTFAPETLTDWEVGTKLRWQAGG
                     ARGSLEVALFSGKDAGNQFGVSTSGLVGGVCVPQAIGSAGRAADCTTQPTQTAYTPGT
                     PGVLIRHPANTTVGNVGDLTIRGFEIGATVSPTRWLTVEAGMAYVDYSVDAITI"
BASE COUNT         1669 a         3140 c         3125 g         1603 t
ORIGIN      
        1 cgccgccggg aaacacggcc aaggtgccat gcatgctgct gcggtgggcg cgggcgtggt
       61 cgccggcgct gcagcgtttc acggcgcggc agagcgcggc gggcgcgccc cgggcggcca
      121 attcgccgtt ccggccgata atggcggaca ggcgcgggta tcattccggg gcgagcgccc
      181 caccttcctt ggcggctgcc cgcccggtct ggcgaagaag tacaacggct gcaatccgcc
      241 cggcctcgtg aaatcgtctg ccgtgccgtg gcagccggac tggtggggct accccgacag
      301 cggctaccgc tatttcgacg gctatctgct ccgcacgtcc ggccccgcgg tgcttggcta
      361 tgttcccctg ttgggcgggc cgctgttcat cggcgagatc tggccggcgg cttatgaaac
      421 cgcgccggtg cccgcctact acagcgacta ttacgacctc ggcccggact atcgctatta
      481 tgacgatacc ttctaccggc tggagccgcg taccaaccgg atcgtcggcg tggccggatt
      541 ccttaccggg gacgaattcg tggtcggtga gccgatgccc gtcggctacg acgtctacaa
      601 tgtgccgtgg gactaccgcg accgctacgc ggacggaccc gacgcctggt accgctacgc
      661 ggatggctac gtctaccgca tcgatccgaa aaccatgctg atcctggccg cgattcagct
      721 gctgacatga aaggaccggg catgatcaag actttgcggg cattcgtcct cgggctggcg
      781 ctgagcggcc tcgccggggg gtgccagcgg cacgatgcca gtcccgaagc gcgggcgagc
      841 ggcggccccg gcgcggctgc cccggcgcaa gtcacgctgg atgccgccat cggcaagtcc
      901 gcgcagttct cggccctggc ggagatcatc cggcagaccg ggcttgcggg cgtgctcggc
      961 ggcaaggcaa gctacaccgt gttcgcgccg accaacgccg cgttcgacac cctcggggag
     1021 ccgggccgcg ccttgcgtca acccgaacag cgcgccgcgc ttgccgctct gctgcgcgag
     1081 cacatcgtgc ccggcatgct cacgctcggt gacctcaacc aggccctgga gcgcagctct
     1141 ggcaagccgc tgaaactgcg ctccatgggc aaaggcacgc tcaccgtttc gcgcgaggac
     1201 gggaagatca tggtgtccgg ggcggatggg gcgaaggcag ccctgaccaa cgacagcgcc
     1261 accgcgcgga acggttcggc cctgggcatc gacggcgtgc tccgaaaggt ggcctagctt
     1321 ccagccgcga tcctcatcag ggccgtcacc ccgggcaaac caggtcggcc ggcgtgctgc
     1381 tccgctctca cgtcccattt gcaagatcaa gccgcggctg ggccggtgct acgcttgcgc
     1441 aagctcggat caggcgacaa cgtgcaagga taacggatgc tccagtcgcg gcgcatctcc
     1501 tcgggaaagg atgtatatga ccggaatgcg gttagacgac cgggtggcta tcgtcaccgg
     1561 tgccggcggc ggtcttggca aggcctacgc cgatctgctg gcctcccgcg gcgccaaggt
     1621 cctggtcaat gacttcggtg gcagcttcga tggtatgggc agcgatgcgc gctatgccga
     1681 agcggctgcc gccgagatcc gtgccaaggg gggcgtcgcc gaggccaacg gcaacagcgt
     1741 ggccacctcg gccggcgcca aggctatcgt cgacgatgcg atggcccggt ggggccgggt
     1801 cgatatcctc gtcaacaatg ccggcattgt gagcgccagc accaccttgc cgaccattac
     1861 cgacgagcag tgggagaagg acatggcggt ctcggcctcc ggcaccttct acatgtgccg
     1921 cgccgtgtgg caggggatga acgaacgcaa ctacggtcgc atcgtcaaca tcgcctcggg
     1981 ctcgtggttc ggcatgggca gcggcttgcc ctatccggcc ggcaagggcg ccgtctgggc
     2041 catgacccgg ggcttggcct cgacgacgaa tgtctcgaag aacaacgtca aggccaacgc
     2101 gatcatgccg atcgccggca gccgcatgac ggccctgatg ggcgaggaga tcaacgccct
     2161 gatgcagcgc gacttcccgg ccagggcggt ggctcccgtg gtggccctgc tcgcccatga
     2221 cgatgcgccg tgcaatggcg agatgttctc ggtcggcgga ggcggctttg cgcgcgtgat
     2281 ggtcgctgtc gcggcgggct atcgcggcac cgacaaggac tggacgatcg aggacgcggc
     2341 cagccacttc agcgaagcca tggacgtgag ccgctacaca gtgccggccg acgcgctggt
     2401 ggatgccgag ctctaccagt cggacgtgcc gtgggatgcc ttccgagcct tcatcgcctg
     2461 accggccggg cagcgcgagc ccgccacatg tccgggaagt ggcgccccgg tcgcagagga
     2521 cttgcaggcc tggtgttcct acccggtcca actacgcggc agcgatgtga acgaccgctc
     2581 gtctccgtca agcctggcat ccacttccag cgcggcgcct gtcaccgggt cgaggtacgt
     2641 atcgtagatc acgtcggtac cgctgcgggt ggggtgacgc tcgcgcaaca ccgggcaacc
     2701 gtccgtccag tggtgggggg ccttggccaa gggggctccg ctcgccatgc tgtaagctat
     2761 cgcgccgcgg cgcccgatcc cgggatagat gacagcctcg gctgccccgg atggcaggaa
     2821 ctccgcgtcg gtcaacgcaa gcgggtgccg ggccggctcg gcccgggcca gccgctcggc
     2881 gcggaccgat tggcggcgac gctcggttgc gggtccgtct actccggtgc tgtcgatcac
     2941 caccccgtag atgttcagcg cggcctctga agtgatccgc ccaagcgcaa ggtcggactg
     3001 cacagcctgc gggtcccgct ccagcgggtc cccataacca ccgcctgatg aggacttgaa
     3061 ctcgaacgat tcccccgcgc gcagttccac gcccatggtg tgattatcga ctgcctcgcg
     3121 tgagccgtcg ggccggtgga tccagagttc ggagccgccg ccgggccgcc cgccggcgac
     3181 gccggagttg ggcaggtgct ccttcttcgc catggtaacg ccgtagatcc tgtcgatcag
     3241 gtgaggctgg aagatcatgt gcatgcccgc gccgccgcgg aacttgcctt ccgaaccgcc
     3301 accgccgccc ggccacttgc cggtgaccag aaccggatac cagtcctcga acacttcgac
     3361 gtcgatcagt tcgaccatgc cgtgctgccc gacgttctga tccacgaggt cgatcccgtc
     3421 gcggtcgcgc gcggcggggg agccgccggt cgtggtgccg tccatcacgg cgaagccatc
     3481 gccgccatag ctccagaccg tgaaggcatc ggaagcataa ttgccctgct gggcggtgac
     3541 gtactggctg caggcaatat ctgccggcga ggcggcaata gcctggacca ggcacacggt
     3601 cgcggcgctc gcggcggttc ccggtacgtc atagtggcag gcgccgcaag gtgccggggc
     3661 cgtggcgttg acgatcgtcc cctcgggaca aaccacctcc accgcttcga agatcgcctg
     3721 gcaggccggg atatcgttga acaggtagtt cccgagcggg gtcgcgatgc cgccctggat
     3781 gaggtagggg cgggaattga tgaagtgatc gcactgcggc gaggcgccgg tgaagtcgaa
     3841 caccatgcgc ttgcccgcga cggtcagccg gcagggcagc ttgaagaact cgttgttcca
     3901 gtccgcatag gcggtcttgc agtagacccc gtcctgcagc gcggcgaccc gctgatcgac
     3961 cgcctccctg gtctgccggt agaaagcgtc gctcatggcg cggaaccgcc tgccgtcctc
     4021 gccgagcgac cgggcgagcc cggcgagctt ctcgctcgag acatggcacc cggcgaccag
     4081 cgcgcgcagg tccatctcga cgaggtccga aatgcgcacg ttgttgagga acatggtcca
     4141 caggtcggtc tgctcgacgc ccttgcgaaa caggcgcacc ggcgggaccc ggacggattc
     4201 ctggaagcac tcggtcgcat ggggggcgaa ggagccgaac accatgccgc ccacgtccat
     4261 caggtgcgcg gtgctggcaa tccatccgac gagctcgccg gcgacataga ccggttcgga
     4321 tacgaacacg tcgttcgggt ggagcgaggc gtcggcatag ggatcgttgc cgaggaacac
     4381 gtcgccctcg gcgatggtgt cgccgtgatg gcggatgacc gatcggacgt gggcgatcgc
     4441 cgcaaagccg ggattggtgc tcagtccgcc gaacgcgatg agcctgcctt ggtgatcgag
     4501 gatggcggtg gcacagtcga gcgcctcgac cactaccgga ctgatggctg tccgctcgat
     4561 gccgtagagg ccttcgttgc cgattgcctc aaggttggag cgccagacct ggagcgcgat
     4621 ggggctgacg gtttcgtcgg cgggagtgag ggtttcgagt tccatcgtct tagccattca
     4681 ccgagatgat cagggttcca ttgtcgtgca actgtgcggt gcaggtcgag ggcacgaaca
     4741 ccgtggtatc gccgccgtcg atcaacgccg gtccgcagag ctgctcgcgg cgccccacct
     4801 ggtcgtagct gaccaccttc accggttgcc ggccgcgggc ctcgtcgacc aggatgttgc
     4861 gcgaaccgtt ggcggccgag ctcctgccga gcgaccgttc gaccgattcc cgcgatgtcg
     4921 ccacggccgc cttggtcgtc cgaccgatgc cgatggcacg aaccgtcgag aggtctggcc
     4981 gggcgccggg cataagggac ccgctgccga accgggagat gtactcctcg cggaagtcag
     5041 cctccagctg tgccacgacg gtgtcgtcga tcttgagact ccggaacggg atggcgagtt
     5101 cccatttctg gcgattgaag cgaagctcgc cctccagctg gtattcgcgc tcgtcgaccg
     5161 ggattccgtc ccgttcgaga tctccgtcga tctccgtcat cagttcggtg agcaggttca
     5221 ccaggacacc ggtatctacc ggcatcctgg cattgacgct gcgtggccgt tcacgcctga
     5281 catcgagtgt ggccgcgccc gttgccgaaa gcacggaagc gttgcgcgag atcaggactt
     5341 cgctggcacc gatggcggcg gcaatttccg gcgtgaacag cgacccgcaa ccgccatagc
     5401 tgacgatcga cttgccgcgc ggatcgattc ccagcttggc aaggcgcccg cgcacagcgg
     5461 caatcatgcc ggcaagggca acttcgcgaa tgcccagagc cactgcttcg ggctcgaggc
     5521 cgagcttctc gcccagcttg gcgcaggctc ccacggctgc ctcgcggtcc agccgcatgg
     5581 tgccgccgag gaacgtgtcc tcatcgagca ggcccagcac cagcagggcg tcggtcactg
     5641 tggcttcggt cccgccgcgg ccgtagcagg ctgggccagg catggagccg gcggagcgag
     5701 gccccacctg aagcgccccg aaggcgttga tggtggcgat cgatccgccc ccggctccga
     5761 tcgagtcaac gtcgacggaa cgcagcgcgg tccagattcc catgagatcg ccccgcaccc
     5821 gccgggccgg ctcgccgcgg ctgagcaccg agaaatcgaa cgtggtgccg cccatgtcgc
     5881 aagtgattgc ctcgctgacc ccgcagctgg cggccagcat cgccgcagcg cccacgcctg
     5941 cggccggccc ggactcggcg agcgagaccg gcacttgccg tgcttcctcg acactggtgg
     6001 ccccgccgcc cgaatggatg agaagcagcg gagcggacag cccccgccgg gcgagttcct
     6061 cgctcagcca gtcgatcccg gccagggcgc cggatgtata gctgttcagc aggcaaaaca
     6121 tcgtccgttc gtattcgcgc accaccggca ggaggctcac ggcggatgac accggcagat
     6181 cgggatagcg ctcccggatc aggcgcacgg cccgcgcctc gtggctgtcg tttcgcaccg
     6241 accagatgaa cgagaccgcc agcgcctcca cttcacgcga ttcgaccagc gtggtcgcgg
     6301 cggcgagcac cgccgtttca tcgagcgggg tgatgacttc gccgcggcga tcgatgcgct
     6361 ccgcaacacc gatgatccgg tcgcgcgaaa tgatctgggg cggcgacacc aaccagccgt
     6421 tctcgctgcc ccagtgcaac ttcgagaagg ggacgatatt ctcgaaccct gccgttgtga
     6481 tcaggccgat ccgacgcccg gcgcgcgagg cgatctggtt ggtcacgacg gtcgtcccca
     6541 gcccgaagcg cttcacgttc gggaggaggt cgtcaagcgc gacgccaagc cgctcggcca
     6601 ccagttcgca ggctgcaatg acgccgcggc ccggatcgcc gtgcgtggtc agcgccttgg
     6661 cgcggatgac ctcgccggcg ccggtcagca ccgcgtcggt gaaagtaccg ccgacgtcga
     6721 tcccgatgca gagattctca agcatggcgc gcaaccccca atccgccttt cctgcaggcg
     6781 ggccgtgccg gctgttcgcc tccgcaaact ggcccagaca aattgaaacg acgctcgcga
     6841 gagtacattc aggcaacctt cccggatttg tataagtcat ttcgcgccac ggcgcgacgc
     6901 ctgactatga ctggtaacga tcacgctttt cccatacaag ccaaaagttg tcataaaata
     6961 taacctgaca tgggtcagaa gagataaatg aacttcattt attttttcgg tccttcttat
     7021 tcttgaccaa ttaaattccg gggagcatcc aagtcataga gaggtctatg aacgcctgcg
     7081 ccagggcgcg cggtcacggc tggtcttatc tgcggggcgg tagcgcgagg gagcagttgg
     7141 cgggagcgtg cacgatcgcc cggggggggg aattgaggtg cgtcagagga atggggggcc
     7201 ttcggctccc ggtggcggtg ctcgggtcac ctcgcatcgg gcgatgggcg accgagaggg
     7261 ccgtcaacta cagaacagcg aaatagtaaa aacaggagag gtgttgtgcg gcgatttatt
     7321 ctgttggttt gtggcgtcag cggccttgcc ttgggcgccg gtccggcttt cgctcaacaa
     7381 ggcgcgagcg gggcggctgc ggacggcgct gatcagcccg aagccattgt cgtaaccgcg
     7441 cgccgccggg cggaagatgt cagcaaggtg ccgatctcga tcagcgcctt ctccagcgcc
     7501 acgctggaga agcgcggggt cacgaacgtt gccgacctgg tgaaaatcac gcccggcctg
     7561 aacatatcgg caggcgccat caagtcgaac ccattcgtga ccatccgcgg ccagagccgc
     7621 ggggttacag gcaacatcaa ccctggcgtg atcacctatc tgaacgaagt gccgctgccc
     7681 acccaggggg cgctgatccc gacctacgac atggacaaca tgcaggtcct gaagggcccc
     7741 cagggcacgc tgttcgggcg caattcgatc ggcggcgcgg tactgatcta caccaaggcg
     7801 cccagcgacg atttcggcgg atatgtaaag ggcgagatgg ccagcttcga tttcaagcag
     7861 atcgaaggtg cgatcaatgt tcccgtcgtc ggtgacaagc tcgtcatccg cctggcgggc
     7921 caggtgtcgt cgggcggcgg ttacaccaag gcgatcgggg tgacccccta cacggtcgat
     7981 cccgtcaccc tggtcgcgac gccgggtcac atccgtccgc tgaagcacaa ttatgacgaa
     8041 tacgacaccg gctcgttccg cgtctcggtt cgcgcggaac cgacggattg gctgaagaac
     8101 gtgacggtgg cggactacac caagatccgc ggcgccaaca acacgctgtt ccacagcttc
     8161 tacccgcagg gcttcggcgg taatccgccg gcgctctaca tgcagccacc cgccaccatc
     8221 acggcattcc tgtccagcat cttcggtccg gatgccggcc cggcctttgc tgccaacatg
     8281 atcgccgtga cccagtgcgg caccagcctg acctgcgact atcgcctggc ccagcaggcc
     8341 ggtcaggcca gcgaccgggt ccagttcacc aatgtcgatc cattcgactc cgtcgcgatc
     8401 gtcaagggta tcagcaatac cacgacgatc cgccttggcg acaagcacac cctgaagaac
     8461 atcttcggct accggtcatt cttctcgtac aacccggtcg ataacgaagg atcggcgatc
     8521 gacgtcgccg acatcgatgc ccagatttcg atggaagaat atacggacga actgcagttg
     8581 tccgggtcgt tcctgaacga caggttgaaa tataccctgg gcgggttcta ctacaaggaa
     8641 caaccgcgag gattgggtgg gaatttcggg actgaataca acgtcttcca gggtctctcc
     8701 catgcccagg tcaccgatta catcaccaac aagagcaagg cgatctacgg ccagttcgac
     8761 tactcgctgg atgatctcat caatggcctg accgtcaccg ccggattgcg ccagacctgg
     8821 gacactgtcg gcggctgcac ggcctcggcg acctactcgc cctttggcca ggtgtactat
     8881 accggcagga acctgaactg gatttcccag gccgattgcc agaagggctc ggggctgact
     8941 acggcgaacg tccccggggc catcatagtc tcggcccaga ccctgcccga agtcgaattc
     9001 aagaagatga cctacacctt cggcgctaac tggcagatca cgccggacgc catggtctac
     9061 gtgacccacc gccggggcta ccgggccggg agctacaaca cgcccctgtt cgatccatac
     9121 ctggccggct cgcagacctt cgcaccggag acgctgaccg actgggaagt ggggaccaag
     9181 ctgcgctggc aggctggcgg cgcgcgcggc tcgctggaag tcgccctgtt cagcggcaag
     9241 gacgcaggca atcagttcgg cgtgagcacc tcggggctgg ttggcggcgt atgtgtgccg
     9301 caggccatcg gctcggcggg ccgggcagcc gattgcacca cccagccgac gcagacggcc
     9361 tatactcccg gtacgcccgg cgtgctgatc cggcatccgg cgaatacaac cgtcggcaat
     9421 gttggcgatc tcacgatccg cgggttcgag atcggcgcca cggtttcgcc aacccgctgg
     9481 ctgacggtcg aggccggcat ggcctacgtc gactattcgg tcgatgcgat taccatc
//
LOCUS       DATNBV010000516         3205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2847809,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000516 DATNBV010000000
VERSION     DATNBV010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2847809"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VN627_12230"
     CDS             <1..803
                     /locus_tag="VN627_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD04064.1"
                     /translation="GSVGGPAAGGLLYAAAPSAPYWAAALLLAVAAASILSIKPFSMP
                     RLKADVHPLRQIGEGFSYVRQHRLLLGCITLDLFAVILAGATALLPVYARDILTWNGQ
                     PVGPAGLGQMRAAPAVGAALVALLLSFRPIERNVGVKMLWAVVAFGLATIVFGLSRNY
                     LLSLAMLVLLGAVDMISVFIRTALVQLSTPDAMRGRIASISGLAISASNELGEMQSGI
                     AAALLGATGAVVFGGIGAIVITATWALIFPELRRSRSFSTNYSERASA"
     gene            835..1752
                     /gene="cysK"
                     /locus_tag="VN627_12235"
     CDS             835..1752
                     /gene="cysK"
                     /locus_tag="VN627_12235"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HXD04065.1"
                     /translation="MKAASILATIGNTPHVRLARLFPDHEVWVKSERANPGGSIKDRI
                     ALAMVEAAEADGSLKPGGTIVEPTSGNTGIGLAMVAAVKGYKLVLVMPESMSLERRRL
                     MLAYGATFDLTPREKGMKGAIERAKEILTEVPGSWMPQQFENAANIAVHARTTAQEIL
                     ADFADSPIDALITGVGTGGHLTACAEVLKEKWSGLKAYAVEPTLSPVISGGQPGPHPI
                     QGIGAGFIPGNLHTAAINGVIQVDPADAKEWARRSAREEGLLVGISSGATLAAIAQKL
                     GDLPAGSRVLGFNYDTGERYLSVPDFLPE"
     gene            1749..2474
                     /locus_tag="VN627_12240"
     CDS             1749..2474
                     /locus_tag="VN627_12240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXD04066.1"
                     /translation="MSTAPTSLERVDYADRGTPLTGWLARPEGTPRAAIVVFPTIVNV
                     NSPIERRARMLADAGFVALIADFYGEPVASFDAAGPLSKALTADPDHFRARLIAAATA
                     LRDHPAAAGLPLAAIGYCMGGQSAIELARAGEDLAFVASFHGTFRTRRKASADALHKP
                     RLLICHGDADPLAPREDVIALWEELSAAGYSWHFHAYSGVKHGFTDPGSDARGLDAIA
                     YDASADRQSWAALMSLADEVFGA"
     gene            complement(2479..>3205)
                     /locus_tag="VN627_12245"
     CDS             complement(2479..>3205)
                     /locus_tag="VN627_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HXD04067.1"
                     /translation="MRALILWCALQVDLETAAATEEERQLAADLVGLLTPVIKGYGTD
                     KGYAVATNMQQVWGGHGFIVENGMEQFVRDARITMIYEGANGVQAMDLVGRKLPANMG
                     RAIQVFFAAVDKECAEAGADARTAPVAERVGKANGELKAATMWLMQNAMAKPDNAGAG
                     AHHYMHIMGIVALGLMWLRMGRTAAAALDAGAGDTAFYEAKLTTARYFAERFTPDAGA
                     LRRKLEAGAETMMALPAEAFATA"
BASE COUNT          481 a         1120 c         1002 g          602 t
ORIGIN      
        1 tcggctcggt cggcggaccg gcagctggcg ggctgctcta tgctgcggcg ccttctgccc
       61 catattgggc agcagccctc ctgcttgccg tcgccgcggc cagtatcctc tcgatcaagc
      121 ctttctcgat gccccggctc aaagccgacg tgcatccgct gcggcagatt ggcgaaggct
      181 tttcttacgt acgccaacac cggctcctgc tcggctgcat cacgctcgat ctgttcgccg
      241 tcatccttgc cggggccacg gcactgctgc cagtctatgc ccgtgacatt ctcacctgga
      301 atggccagcc ggtaggcccg gccggcctcg ggcagatgcg cgccgctccg gctgtcggcg
      361 cggcgctggt cgcgctactg ctgtccttcc ggcccatcga gcgcaatgtc ggcgtgaaaa
      421 tgctgtgggc ggtggttgcc ttcggtctgg caacgatcgt gttcggcctc tcgcgcaatt
      481 acctgctgtc gctcgccatg ctggtcctgc tcggcgcggt tgacatgatc tcggtcttca
      541 ttcgcaccgc cctggtccag ttgagcacgc ccgatgccat gcgcgggcgg atcgcctcca
      601 tttctggcct ggccatctcg gcatccaatg agctggggga gatgcagtct ggtatagctg
      661 ccgccctgct cggtgcgacg ggggcagttg tgttcggcgg gatcggtgcg atagtaatca
      721 ctgccacctg ggccctgatc ttcccggagc tgcgccgttc gcgcagcttc tccaccaact
      781 attcagagcg ggcatccgca tgatttctta ttcttgcaca aaggagccaa tctcatgaaa
      841 gcggcatcca tcctggccac gatcgggaac accccgcatg tccgcctcgc ccgcctgttc
      901 ccggaccacg aggtctgggt gaagagtgag cgcgccaatc ccggcgggtc gatcaaggat
      961 cgtattgccc tcgccatggt ggaagccgcc gaggcggatg gcagcctcaa acccggcggc
     1021 accattgtcg agccgacctc gggcaacaca ggcattggcc ttgccatggt ggcggcggtc
     1081 aagggctaca agctggttct cgtcatgccc gagtccatgt cgctggagcg ccggcggctg
     1141 atgcttgcct atggcgcaac cttcgatctc accccccgcg aaaagggcat gaagggcgcg
     1201 atcgagcggg ccaaggaaat cctcaccgaa gtccccggct catggatgcc gcagcagttc
     1261 gaaaatgccg cgaacattgc cgtgcatgcc cgcaccacgg cgcaggaaat cctggctgac
     1321 tttgcagata gcccgatcga cgccctgatc accggtgtgg gcacgggcgg ccatctcacg
     1381 gcctgtgccg aagtgctcaa ggagaagtgg tcaggcctca aggcctatgc ggtcgagccc
     1441 accctttcgc cggtaatctc gggcggtcag ccggggccgc atccgatcca gggcatcggt
     1501 gcgggcttca ttccgggcaa tctgcacact gcggcgatca acggcgtgat ccaggtcgat
     1561 ccggccgatg ccaaggaatg ggcccgccgc tcggcgcgcg aggaagggct gcttgtcggc
     1621 atcagctcgg gcgcgacgct ggctgccatc gcccagaagc ttggagacct gcctgccggc
     1681 agccgcgtcc tcggcttcaa ctacgacacc ggcgaaaggt acctctcggt cccggacttc
     1741 ctgcctgaat gagcaccgcc cctacctcgc tcgaacgcgt tgactatgct gatcggggga
     1801 cgccccttac cggatggctg gcccgtccgg aggggacccc gcgcgccgcg atcgtggtgt
     1861 tcccgaccat cgtcaatgtg aactcgccaa tcgaacgccg cgcacggatg ctggccgacg
     1921 ccggttttgt cgcgctgatc gccgacttct acggcgagcc ggttgcgtct ttcgatgcag
     1981 ccggcccgct gtcgaaggcg cttaccgccg atcccgatca tttccgcgcc cgcctcattg
     2041 cggcggcgac tgcccttcgc gatcacccgg ccgccgcggg cctgcccctg gcggcaatcg
     2101 gctattgcat gggcggccag tcggccatcg aactggcccg cgccggggaa gaccttgcct
     2161 tcgtcgccag ctttcacggc accttccgca cccgccgcaa ggcctcggcc gacgccctgc
     2221 acaagccgcg cctgctcatc tgccacggcg atgccgatcc cctcgccccg cgcgaggatg
     2281 tcatcgctct gtgggaagag ctcagcgccg cgggctattc ctggcacttc catgcctatt
     2341 ccggcgtgaa acacggcttt accgatccgg gcagcgatgc ccgcggcctc gatgccatcg
     2401 cctatgatgc cagcgccgat cgccagtcgt gggcggcgct gatgagcctg gcggacgagg
     2461 tgttcggcgc ctgatcgctc aggcggtggc gaaggcttcc gccggcaggg ccatcatcgt
     2521 ttccgccccg gcttccagct tgcgccgcag cgccccggcg tcgggggtga agcgctcggc
     2581 aaaatagcgg gctgtggtca gtttggcctc gtagaacgcc gtatcgccgg ctcctgcatc
     2641 gagtgccgcc gccgccgtgc ggcccatccg cagccacatc agcccgagcg caacgatgcc
     2701 catgatgtgc atgtagtggt gcgcgcccgc gccggcgttg tcgggcttgg ccatggcgtt
     2761 ctgcatcagc cacatggtag cggccttgag ctcgccattg gccttgccca cccgctcggc
     2821 cacgggggcg gtccgggcat cggctccggc ttcggcgcat tccttgtcga ctgcggcgaa
     2881 gaacacctga attgcccggc ccatgttcgc gggcagcttc cggccgacca gatccatggc
     2941 ttgtacgcca ttggcgcctt catagatcat cgtgatccgc gcatcgcgga cgaactgctc
     3001 catgccgttt tcaacgatga agccgtgccc gccccagacc tgctgcatgt tggtggccac
     3061 ggcgtagccc ttgtcggtgc cgtaaccctt gatcaccggt gtcagcagcc cgaccagatc
     3121 ggcggcaagc tggcgctcct cctccgtcgc cgccgcggtc tcgagatcga cctgcagcgc
     3181 gcaccacagg atcagcgccc gcatg
//
LOCUS       DATNBV010000517         4370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3099564,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000517 DATNBV010000000
VERSION     DATNBV010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3099564"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..958)
                     /gene="fmt"
                     /locus_tag="VN627_12250"
     CDS             complement(50..958)
                     /gene="fmt"
                     /locus_tag="VN627_12250"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXD04068.1"
                     /translation="MRVIFLGTPDFAVPTLVALVEAGYEVACVYTQPPRPGGRRGKEL
                     TPSPVQREAERRGIAVRYPVSLRSVEAQEQFAALNADIAVVAAYGLILPQAVLDAPRH
                     GCLNVHGSLLPRWRGAAPVQRAILAGDAETGVGIMRMERGLDTGPVLLEGRTAVDRKT
                     AGELTDELAAMGAGLMVRVLADLAAFPEVPQPEDGVTYAHKIDKAEARIDWTRSAVEI
                     ERQVRAFNPAPGAFFELDGERCKVLAAEVAAGSGRPGTVLDDALTVACGDSALRLARL
                     QRAGRPTMDAADLLRGWAIPPATVLA"
     gene            1118..1714
                     /gene="recR"
                     /locus_tag="VN627_12255"
     CDS             1118..1714
                     /gene="recR"
                     /locus_tag="VN627_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXD04069.1"
                     /translation="MASQEIETLAGALARLPGLGPRSARRAVLWLIKRRESALPALLE
                     ALAQVQGALVECERCGNVDTTSPCGICADPRRDPRSLCVVEDVADLWALDRAHLFTGR
                     YHVLGGRLSALEGVRPEDLTIDRLLARVAEGGIDEVVLAMNATLEGQTTAHYLAERLE
                     QFPVRLTQLAHGLPVGGELDYLDEGTLAQALRARRPLG"
     gene            1776..1870
                     /gene="ffs"
                     /locus_tag="VN627_12260"
     ncRNA           1776..1870
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VN627_12260"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1934..3799
                     /locus_tag="VN627_12265"
     CDS             1934..3799
                     /locus_tag="VN627_12265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXD04070.1"
                     /translation="MSDSTDMFGDDPTDNDPPWDTGEEDRASAAELEAAGQSSMFGDP
                     APTPAPVPVTPPAPPPAAAAAAPTPVPAAAQPYRVLARKYRPQTFSALIGQDAMVQTL
                     ANAIRRDRLAHAFLMTGVRGVGKTSTARLIAKALNCVGPDGQGGPTIDPCGRCEPCTA
                     IAEGRHIDVIEMDAASHTGVDDVREIIEAVRYAAVSARYKIYIIDEVHMLSRNAFNAL
                     LKTLEEPPAHVKFLFATTEVDKLPVTVLSRCQRFDLRRITTGLLAEHFARICAEEGVE
                     ADPEALSMIAEAAEGSVRDGLSILDQAIAHADLGGEGRVTPAQVQDMLGLADKSVQRK
                     LLAALLGGDAPAVLDLVEAQFALGVEPLALLRSQMALVHRITVAQVSRANARAISPEE
                     RAAVDEWSGKLSAAQLHRLWQLLLKGFEEVRSAPDPLVAAQMALLRVLHAADLPDPGQ
                     LVRKLESLAANPAAFTAPIPAGEGAPAATPTAAASPVSATDWAALVEQVEHGSPMLGA
                     SLRLGVRIVSLAPGLLRYQLAPGLAPLADADIRKGLDNATGLSWQVERVTDGEAAPSL
                     EEARVAAESQARESLRRSPLVEAAFAAFPGAELIEEEAASPSRGGPGERNWSRRA"
     gene            3796..4137
                     /locus_tag="VN627_12270"
     CDS             3796..4137
                     /locus_tag="VN627_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXD04071.1"
                     /translation="MKSMEEMIQAAQKAAETIQQQMNQAQGKLDQIEVEGLAGGGLVK
                     IRCSAKGRVIGVAIDESLLQPSEKGILEDLVAAAFNDARARADKVTGEEMAKMQQGLG
                     LPPGFNLPGFG"
BASE COUNT          657 a         1530 c         1490 g          693 t
ORIGIN      
        1 cgcgcgctgg gccggggtgg cgtggatcgt tgtcatcggc tgtcctccct tacgccagta
       61 ccgttgcggg ggggatggcc cagccgcgca gcaggtctgc cgcgtccatg gtcgggcggc
      121 ccgcccgctg gaggcgcgcc agccggagcg cgctatcgcc gcaggctacg gtcaaggcgt
      181 cgtcaagcac ggtgcccggc cgccccgacc ccgccgcgac ctcagccgcg agtaccttgc
      241 atcgctcccc atcaagttca aagaacgccc cgggcgccgg attgaacgcg cgcacctgcc
      301 gctcgatctc caccgcgctg cgggtccagt cgatccgggc ctcggccttg tcgatcttgt
      361 gggcgtaggt cacgccgtcc tccggctgcg gcacttcggg gaaggcggca aggtcggcca
      421 gcacccgcac catcagcccc gcgcccatgg ccgccagttc gtcggtcagt tcgccagctg
      481 tcttgcggtc aaccgctgtg cgcccctcca gcagcaccgg cccggtatcc agcccgcgct
      541 ccatccgcat gatcccgacg ccggtctcgg catcgccggc gaggatcgcg cgctgcactg
      601 gcgccgcccc gcgccagcgc ggcagcaggc tgccgtgcac gttaagacag ccgtgcctcg
      661 gcgcgtccag cactgcctgg ggcaggatca gcccataggc ggcgacgacg gcaatatcgg
      721 cgttcagcgc ggcgaattgt tcctgcgctt ccacgctccg gaggctgacc ggataacgca
      781 ccgcgatgcc ccgcctttcc gcctcgcgct gcacgggcga gggggtcagc tccttgccgc
      841 gccggccgcc ggggcgaggg ggctgggtat agacgcaggc gacctcgtac ccggcctcga
      901 ccagcgctac cagcgtcggc acggcaaagt ccggcgttcc caagaagatc acgcgcacca
      961 gtcagcttcc atctgtcttg cccccctccc ggaggggagg gctgggggag ggggaaggca
     1021 cccgaatgca ccgcccagca cccccaccca gcctccccct caagggagag gggcttttcg
     1081 gttcaaattt cctgcgtccg gccctatctg tcgcgctatg gcatcgcaag agatcgagac
     1141 actggccggc gcactcgccc gcctgcccgg gctggggcca cgctcagccc gccgcgcggt
     1201 gctgtggctg atcaagcggc gggagagcgc cttgccggcc ctgctggagg cgctagctca
     1261 ggtgcagggg gcactggtgg agtgtgagcg ctgcggcaat gtcgatacga ccagcccttg
     1321 cggcatctgc gccgatccgc gccgcgatcc gcgctcgctg tgcgtggtgg aggacgtggc
     1381 cgacctttgg gcgctggacc gggctcacct gtttaccggc cgctaccatg tgctgggtgg
     1441 gcggctttcg gcgctggaag gggtgcggcc ggaggatctg acgatcgacc gcctgctcgc
     1501 ccgggtggct gagggcggca tcgacgaggt ggtgttagcg atgaatgcga cgctggaggg
     1561 gcaaacgacg gcgcattacc tggccgaacg gctcgagcag tttccggtgc ggttgacgca
     1621 gctggcgcat ggcctgccgg ttggcggcga gctggattac ctggacgagg ggacgctggc
     1681 tcaggccctg cgggcgcggc ggccgctggg gtaagccgga ttacaggttt ggcggtccta
     1741 ctggacaaac cgcccacccg ccgccatatg cccgcccggg agtcggtcgg acgcttgcgt
     1801 ccgccaacct ggtcaggtcc ggaaggaagc agccacaacg ggttgcggcg ggtcggccgg
     1861 ctccttttaa atccccgcat ggttgtggcg ctccaatcga caatccgggg ctgcgcgcct
     1921 aactagggca gcgatgagcg attccaccga catgttcggc gatgatccga cggacaacga
     1981 tccgccgtgg gataccggcg aggaagaccg cgcctcagct gccgagcttg aggccgcggg
     2041 acagtcgtcg atgttcggcg atccggcgcc gacgcccgcg cctgttcccg ttaccccgcc
     2101 cgcgccgcct cccgccgcag cagcggcggc tccgacgccc gtcccggccg ccgcgcaacc
     2161 ttaccgcgtc ctcgcccgca agtatcgtcc gcagaccttc tcggccctga tcggccagga
     2221 tgcgatggtg cagacgctgg ccaacgccat ccggcgggac cggctggctc acgcattcct
     2281 gatgaccggc gtgcgcgggg tcggcaagac ctccaccgcg cggctcatcg ccaaggccct
     2341 gaactgcgtc gggcctgacg ggcagggcgg gccgacgatc gatccctgcg gccggtgcga
     2401 accgtgcacc gccatcgccg agggccgcca tatcgacgtg atcgagatgg acgcggccag
     2461 ccacaccggc gtcgacgacg tgcgagagat catcgaggcg gtgcgctatg ctgccgtctc
     2521 cgcgcgttac aagatctata tcatcgacga agtccacatg ctctcgcgca acgctttcaa
     2581 cgcgctgctc aagacgctgg aggaaccacc ggcccacgtg aagttcctct tcgcgacaac
     2641 cgaggtggac aagctcccgg taacggtcct ctcccgctgc cagcggttcg acctgcgccg
     2701 cattaccacc gggctgctcg ccgagcattt cgcccggatc tgcgccgagg aaggcgtgga
     2761 agccgatccc gaggccctct ccatgatcgc cgaggccgcc gaaggctcgg tgcgcgacgg
     2821 gctttcaatc ctcgaccagg ccattgccca tgccgatctc ggcggggaag ggcgggttac
     2881 tcccgcccag gtgcaggaca tgcttggcct tgccgacaag tcggtccagc gcaagctgct
     2941 ggccgccctg ctcggtggcg atgcgcccgc cgtgctcgac ctggtcgagg cccagttcgc
     3001 cctcggggtt gaaccgctcg cgctgctccg atcgcagatg gcgctggttc accgtatcac
     3061 cgtggcccag gtcagccggg ccaatgcgcg ggcgatttcg ccggaagagc gcgcggcagt
     3121 cgatgaatgg tcgggcaagc tttccgccgc gcagcttcac cgcttgtggc agctgctgct
     3181 caagggcttc gaggaagtcc gcagcgcccc cgatccgttg gttgcggcgc agatggcgct
     3241 gctgcgcgtg ctccatgctg ccgacttgcc ggatccgggc cagctcgtgc gcaagctgga
     3301 gagccttgcc gccaatccgg ctgccttcac cgccccgatc ccggcgggcg agggcgcgcc
     3361 tgccgctacc ccgactgctg ccgcctctcc cgtttcggcg accgactggg cggcgctcgt
     3421 ggaacaggtg gagcatggct cgcccatgct tggggcgtcg ctgcgccttg gcgtgcgcat
     3481 cgtcagcctg gcgccggggc tgctgcgtta tcagcttgcc ccgggccttg ccccgctggc
     3541 cgatgccgat atccgcaagg ggctcgacaa tgctacgggc ctgagctggc aagtggaacg
     3601 cgtcaccgat ggagaggccg cgccgagcct tgaggaagcg cgcgttgccg ccgaaagcca
     3661 ggcgcgggaa agcctgcgcc gctcaccgct ggtggaagct gccttcgcgg catttcccgg
     3721 cgccgaactg atcgaagagg aagccgcttc gcccagccgt ggaggtccgg gcgagcgcaa
     3781 ctggagccgt agagcatgaa gtcgatggaa gagatgatcc aggccgccca gaaggccgcc
     3841 gagaccatcc agcagcagat gaaccaggcc cagggcaagc ttgaccagat cgaggtcgaa
     3901 gggcttgccg gcggcgggct ggtgaagatc cgctgctcgg cgaagggccg tgtcatcggc
     3961 gtcgccattg atgaaagcct gctgcagccc tcggaaaagg gcatcctcga agacctggtc
     4021 gccgccgcct ttaatgacgc ccgtgcccgg gccgacaagg tcaccggcga ggaaatggcc
     4081 aagatgcagc agggcctcgg cctgccgccg gggttcaacc tgcccggctt tggctgatcg
     4141 gggaacgact gtcgcgcaga ggcgcaaagg gattgcgacg cagccgcttt ccctcgcagg
     4201 catcgcgggt ttggcggcct ggcgatttcg agagggctac gcccgaccgt tctctgcgcc
     4261 tcagcgcgaa actcttgctc ctgaggctct ctcggtggcg cccggtgggc gatcatccac
     4321 aagtcccgcc ggccccgcca ttgcaccact caggggtcct ccccatatct
//
LOCUS       DATNBV010000518         4127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1579336,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000518 DATNBV010000000
VERSION     DATNBV010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579336"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..597
                     /locus_tag="VN627_12275"
     CDS             202..597
                     /locus_tag="VN627_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04072.1"
                     /translation="MKRTSIKRYTPLAPTRMVRKRQRRIDRETPAEKTHKRLIRRMTW
                     CLARDFGPCEGPLQAAHLARSRGTGLPHGTQEDTAMLCLSHHDQYDGRAKGPLRDVPH
                     AERMELGADWIREAREFVAHVAAAEEAPF"
     gene            612..788
                     /locus_tag="VN627_12280"
     CDS             612..788
                     /locus_tag="VN627_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04073.1"
                     /translation="MTTDTKAAQGEAMSFRLRFWLAAMDLASLMRLPRPIYLWCVVRA
                     SNATDWGDGNGARP"
     gene            785..1825
                     /locus_tag="VN627_12285"
     CDS             785..1825
                     /locus_tag="VN627_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04074.1"
                     /translation="MTTKPPSGESAPQVGDRVMWTSQAAGTTLEKVGIVEKVVAAGEF
                     IRILEHGLYGQRKHESYLVRVDRGPGRQSKLYWPRVAGLRLVSHAAPVPAVGTREGLT
                     FAEFSAANRLRCERDFGRTLGTRDAAVSMCIGLAEESGEVAGAVRAYLGISERKNPSV
                     EAVGNEIADLVSYADLLAQTVGLSLADALVRKFNIVSGRIGSDVRLAAPTGELAADPM
                     DRPGLPWVEQCHCGSGKAPHRFGKHESCESYKHHCDTCHGSRSCTLHECIAAFNSGEP
                     ARRDTIEQVARWFDLEATNLHAVAVDMTAERAGLRRATAAVLAGFAAKLRDGSWLRDL
                     AALEKGDATDGK"
     gene            1815..2156
                     /locus_tag="VN627_12290"
     CDS             1815..2156
                     /locus_tag="VN627_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04075.1"
                     /translation="MENETTTPERSKDSIETHIRCKCSKCGHEGDVWIDPTPLFVAKI
                     NEMVLDGLSIANERIEAARRALKRALDFQGVNDRTLAIKTEVRAALDVIGWSDTPSPA
                     PDAGQTTKGTL"
     gene            2727..3458
                     /locus_tag="VN627_12295"
     CDS             2727..3458
                     /locus_tag="VN627_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04076.1"
                     /translation="MTTVTVAPEKLAILDTLAINKSAHESFDQGHCAMELVAWLAGEP
                     HSDAPACACPVISRAVIRLNDRMPSDEMRAELLRPLLPRIVGSRSTRVVQVKRAYIAA
                     DMACRVFAPMALEARGKLDLAAGLRALAPITDTETAKAAKEKCRAAAAAAAYAAYAAA
                     AAAAYAAADDAYAAAAYDAAAAAAAAAAYDDAAAAAAAAAAYDAAAAAAAAAAYDAAA
                     ADAAARKQVFTEGVRMIGLMLDVRE"
     gene            3699..3971
                     /locus_tag="VN627_12300"
     CDS             3699..3971
                     /locus_tag="VN627_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04077.1"
                     /translation="MSIRPARETKRMTDEELSRVLTAHEAGGLQRGGNGRVVGYPCCI
                     QQARWSIQKLPTRQVFTDITEWFDSNYQSTWTADEFLRQLERRGLA"
BASE COUNT          762 a         1363 c         1401 g          601 t
ORIGIN      
        1 ggtcgtcgtt gagcgtgaga tgggcgaccc cgcatttagg ccctggccgc ttcgctgcaa
       61 gaagctgagc cggcggggcg agacgtgcag ggccatcacc gccgatgcaa tggaggccgc
      121 tctttgggag agcgtgacgc acctactgac ggaggcgatg gagcggcgaa tgcggcccgc
      181 gactgggaag ggcgggaagc cgtgaagcgc acctccatca agcgctacac gcccctggcc
      241 ccgacgcgga tggtccgcaa gcgccagcgg cgaatcgacc gcgagacgcc agccgagaag
      301 acgcacaagc gcctgatccg gcggatgacg tggtgcctgg cccgcgactt cggcccctgc
      361 gaggggccgc tgcaggccgc ccacctggcg cgctctcgcg ggacgggcct gccccacgga
      421 acgcaggaag acaccgcgat gctgtgcctg agccaccacg accagtacga cgggcgggcc
      481 aagggaccgc tgcgagacgt gccgcacgcg gagcggatgg aactcggggc cgattggatt
      541 cgagaggcgc gggagttcgt tgctcacgtt gccgcagcag aggaagcgcc gttttgacca
      601 ggaggagaga catgaccacc gacaccaagg ccgcccaggg agaggctatg agctttcgtc
      661 ttcgcttctg gctggccgca atggacctcg ccagcctaat gcgtctgccg cgtccgatct
      721 atctgtggtg cgtcgttcgg gcgagcaacg cgaccgattg gggcgatggg aacggagcgc
      781 ggccatgacc accaagcccc cgtccgggga gagcgcgccg caggttgggg accgagttat
      841 gtggacctcg caagccgcag gcacaaccct ggagaaggtt ggcatcgtcg agaaggtcgt
      901 cgcggccggc gagttcatca ggattctcga gcacggtctg tacgggcagc gcaaacacga
      961 atcgtacttg gtacgcgttg accgaggccc cgggcggcag tcgaagctct actggcctcg
     1021 cgtcgctggc ctgcgcctcg tctcccacgc cgcccccgta ccagccgtgg ggacgcgaga
     1081 ggggctgacg ttcgcggagt tctcggcggc gaaccggctg cgttgcgagc gggacttcgg
     1141 acgcacgctc ggaacgcgag acgccgccgt gtcaatgtgc atcggcctcg cagaggaatc
     1201 cggcgaagtg gcgggggccg tgcgggccta tctcggaatc agcgaacgca agaacccgag
     1261 cgtcgaggct gtcgggaacg agatcgccga tctggtctct tacgctgacc tgctggcgca
     1321 gacggtcggg ttgtcgctcg ccgacgcact cgtgcgcaag ttcaacatcg tttcggggcg
     1381 cattggctcc gatgtccgcc tagccgcccc caccggggag ctggccgccg acccgatgga
     1441 ccggcccggt ctgccgtggg tcgagcagtg ccattgcggc tcaggaaagg ccccgcaccg
     1501 gttcggcaag cacgaatcct gcgagagcta caagcaccat tgcgacactt gccacgggtc
     1561 ccgctcgtgc acgctgcatg agtgcatcgc tgcgttcaac tccggagaac cggcgagacg
     1621 ggatacgatt gagcaagtag cgaggtggtt tgatttggaa gccaccaatc tgcatgcggt
     1681 agccgtggac atgacagccg aacgcgctgg cctgcgacgc gcgactgccg ctgtgctcgc
     1741 gggattcgcc gccaagctcc gcgacggctc ctggctccgt gacctcgctg cactggagaa
     1801 aggagacgcg accgatggaa aatgagacga cgacgccgga gcggtcgaag gattcaatcg
     1861 aaacgcacat tcgctgcaag tgctcgaagt gcggccatga aggcgacgtt tggatcgacc
     1921 cgacgccgct tttcgtcgcg aagatcaacg agatggttct ggacggtctc tccatcgcca
     1981 acgaacgcat agaggccgcc cgccgcgcat tgaagcgcgc gctcgacttc cagggcgtaa
     2041 acgacaggac cttagcgatc aagaccgagg tccgcgctgc cctcgatgtc ataggctggt
     2101 ccgatacgcc gagtcccgcc cccgatgcgg gacagacgac gaaggggacg ctgtgatcaa
     2161 acgtctgcgg gcgcttggcg ggacatgggt tgctgagcgc accggctcgt ttggcaactg
     2221 gcggtactcg ggctcttatg gcggcgctgc ctgggatgtt cggtcatacg cgcacttggc
     2281 cccgcgttac gacggagacg atgagacgtt cgttgttcgt tggcacatcg aaagaaacgg
     2341 tgtgccgtgg gggtacccga ctagcgaccc tgtgactgca atcggggccg gcatgacgga
     2401 cctcgccatc ccacccaccg aggcagggac gccggggaag ggggaggggc cgtgaaaaac
     2461 cgagcgttcg attcagttga ggcggcgtac gccgcgggcc gaaagatggg gcgcaaagag
     2521 cggcgcgcgg ccagcgcaaa ggcgtctggt attgcgcggt gtccagactg cggctgcaag
     2581 gactggtcat gcggacacgc tgacaccgga acggtggcct gtctgaccgg tgctgtccaa
     2641 ttcgacgacg ccaccccgtc ccccactccg ccagtaccga gagggacgcg atgacctcgt
     2701 ccgccacaac ttcacaggag accacaatga caaccgtaac cgtcgcccca gagaagctcg
     2761 caatcctgga caccctggcc atcaataaga gtgcgcacga atcattcgac caaggccact
     2821 gcgcgatgga actagtcgca tggttggcag gggagccgca ctccgacgcg cccgcgtgcg
     2881 cctgcccggt gatttcgcgc gcggtgatcc gcctcaacga ccgcatgccg tcggacgaga
     2941 tgcgcgccga attgctgcgc ccgctactgc cgcgcatcgt tggcagccgc tcgacgcgcg
     3001 tggtgcaggt caagcgcgcg tacatcgctg ctgacatggc ctgtcgggtc ttcgcgccga
     3061 tggcactgga ggctcgcggt aagctggacc tggcggccgg cttacgcgcg ctcgcgccga
     3121 tcacggacac ggaaacagct aaggcggcga aggagaagtg ccgggccgcc gccgccgccg
     3181 ccgcctacgc cgcctacgcc gccgccgccg ccgccgccta cgccgccgcc gacgacgcct
     3241 acgccgccgc cgcctacgac gccgccgccg ccgccgccgc cgccgccgcc tacgacgacg
     3301 ccgccgccgc cgccgccgcc gccgccgcct acgacgccgc cgccgccgcc gccgccgccg
     3361 ccgcctacga cgccgccgcc gccgacgccg ccgctcggaa gcaagtcttc acggagggtg
     3421 tccgcatgat cggactaatg ctggacgttc gggaatgacc gcccagccca cccctaagga
     3481 gcgccgaccg tgagcgccgt gaaactgtgc cccaaataca ccaaggcgag cccgtgttcc
     3541 ggctcggtgg tcgtcatcga gtgcaccgag gacgttggcc gcagcgagta caaggcgtgc
     3601 ggcgagaagt acgaccgttg tgaggcgcac ggtgggttgc ctggggctcg gcggtcgttg
     3661 aaatcgcacc gagggattta ccacccagcg gagcgcccat gagcatccgc ccagcacgcg
     3721 agaccaagcg tatgaccgac gaggaactgt cgcgggtgct gacggcgcac gaggcgggcg
     3781 gccttcagcg cggcggcaac ggcagggtcg ttggataccc gtgttgcatt caacaggcga
     3841 gatggtcgat tcagaaacta ccgacgcggc aggttttcac agacataacc gaatggttcg
     3901 atagcaacta tcagtcgacc tggaccgccg acgagttcct tcgccaactt gaacgaaggg
     3961 gcctcgcatg accactccag tccaagcgac ggttacgccg gaggaccggg aggccgcgca
     4021 cgcggcaatg ggcggcaacc ctgagcgctt cggaaacctg tgtcgcaacc agcactgccc
     4081 agcgtgcgac cgcatcgccc aagccctcgc caacgcacgg gaggcag
//
LOCUS       DATNBV010000519         2127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3493652,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000519 DATNBV010000000
VERSION     DATNBV010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3493652"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VN627_12305"
     CDS             <1..1099
                     /locus_tag="VN627_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HXD04078.1"
                     /translation="LAAEPGPSERVPVWLLAITFAADAIWEDRLALAWVARCEDLARR
                     TGAMRPLILCLIGASITNGCRGQLALAEQQVAEGRELGRALGWSAGQMRSFSGIRNIA
                     FGGDRQALRRLVASGRAIVAARGSGDMIRIALAAQAILHLGYGEYAEAYETATTMRRD
                     DTIGMSAEALPAVVEAGLRSGHATEAAAALADLERCATASGTGWALGLLTRSQALMAP
                     PDQARKLYQESIDILGRTTATADLARAHLLFGEWLRRNQRPADARNHLQTALSIFTSM
                     GAIPFARRARSELRAAGASPDALQTPNSPLTARESQIASMAAAGYANQEIADRLFITT
                     HTVEYHLKKVFRKLGIVSRRQLRDKVSSEPN"
     gene            1597..1773
                     /locus_tag="VN627_12310"
     CDS             1597..1773
                     /locus_tag="VN627_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04079.1"
                     /translation="MLAVTSGGDIASQMFESTSGLIVDSGRSIDHGFASTGRYGTVCS
                     GALVTVGFQGGRAR"
     gene            complement(1889..>2127)
                     /locus_tag="VN627_12315"
     CDS             complement(1889..>2127)
                     /locus_tag="VN627_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04080.1"
                     /translation="SLRPVVVALAAWGNTRLAPGERSMVLVDAQTGAEAEPVLVDRAT
                     GRRVDGPDFVFTAGPAASRPFQDRYAAHPKPPEP"
BASE COUNT          366 a          742 c          694 g          325 t
ORIGIN      
        1 gctggccgca gagcccggcc ccagcgaacg ggtcccggtc tggctgctgg cgatcacctt
       61 cgcggcggac gcgatctggg aagacagact cgccctcgca tgggtggccc gctgcgagga
      121 cctggcccgg cggaccggcg cgatgagacc gctgatcttg tgcctgatcg gggcgtctat
      181 cacgaacggt tgccgcggcc aactggctct cgccgaacag caggtcgctg agggccggga
      241 gctgggacgg gcactggggt ggagcgccgg gcagatgcgc agtttctccg gaatccgcaa
      301 catcgcgttc ggcggcgacc gtcaggccct gcgccgcctc gtcgcgtccg ggcgggcaat
      361 cgtggctgcc cgcggatccg gcgacatgat ccggatcgcg ctcgcggccc aggccatcct
      421 ccacctcggc tacggggaat acgccgaagc ctacgagacg gccaccacga tgcgccgtga
      481 cgacaccatc ggcatgagtg ccgaggccct gcccgccgtc gtcgaggccg gcctgcggtc
      541 aggccacgct accgaagccg ccgccgccct ggcagacctc gagcgctgcg ccaccgccag
      601 cggcaccggc tgggccctcg ggctactcac ccggtcccag gcgctgatgg ctccgcctga
      661 tcaagcccgc aagctctacc aagagtcgat cgacatcctc ggccgcacga ccgcgacagc
      721 cgacctggcg cgtgcgcacc tactgttcgg cgagtggctg cgccggaatc aacgcccagc
      781 agatgcccgg aaccacctcc agaccgcgct cagcattttc acctcaatgg gcgccatccc
      841 gttcgcccgc cgggcccgca gcgagctccg ggccgctggc gcaagcccgg acgcactaca
      901 gaccccgaac tcgccgctca ctgcgcgcga atcccagatc gcatccatgg ccgccgccgg
      961 ctacgcaaac caggaaatcg ccgaccggct gttcatcacc acccacaccg tcgagtacca
     1021 cctgaagaaa gtcttccgta aactcggcat cgtctcccgc cggcagctgc gcgacaaagt
     1081 cagcagtgag cccaattgaa cggcaccatt ccaatggccc gccacccgtc ctaatcacat
     1141 tgacccttcg gctcctgccg ctgaaccact atcgaacccc acccgccgca aagccttcgc
     1201 cagccccgtc ggcagagagc gccgtgtggc agcaaaagca tccccgctcg ttcccatagg
     1261 catggaagca ccccataaca tccaaatctg gcggcactcg ccttgaccaa atcttgatcc
     1321 aagatcacac ctatcggcgg ctattcgtgc ctgagccaag agcgggagta atcacaggaa
     1381 gtgacgttga gcctgggcgg cgatgcgcct gccgattagg cgcttattgg tactgagcta
     1441 gcactcaggg ccgtcgtgct cagatcgtcc agttcggcgc gctgcgcgac cctgccagcg
     1501 gatgtcatgc aggcgactga cgggtccggg agggatctga aagcctcgat tggcgatcgc
     1561 cgccaaaaca ttaaccgcgc ataaggcgga tctcgcgtgc tggccgtgac cagcggtggt
     1621 gacattgcgt cgcagatgtt cgagagcacg agcggtctta tcgtcgactc ggggcggtct
     1681 atcgatcatg gcttcgcatc gaccgggcga tacggcactg tctgttctgg cgcgctggtg
     1741 acggtcggtt ttcagggtgg ccgcgcgcgg tgatcatcgg aaaccgaagc cgagggcaga
     1801 tagccaagcg tcacgtcagc cgtggctgtc ggcgtgctcc ggggagcagt tcgagaaccc
     1861 gggaggggtc ctgcggggca ggcagatgtc atggttccgg cggcttgggg tgcgctgcgt
     1921 agcggtcctg gaatggcctg ctggcggcgg ggccggcggt gaagacgaag tccggcccgt
     1981 ccacgcgccg cccggttgcg cggtcgacga ggaccggttc ggcctcggcc ccggtctggg
     2041 cgtccaccag gaccatgctg cgctcaccgg gggccaggcg cgtgttgccc caggcggcca
     2101 gggcgacgac gacgggccgc agggaac
//
LOCUS       DATNBV010000520         4861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4763267,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000520 DATNBV010000000
VERSION     DATNBV010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4763267"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VN627_12320"
     CDS             complement(<1..276)
                     /locus_tag="VN627_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD04081.1"
                     /translation="MIAGFIMALSNFMVILDLTIANVAVPHIAGNLGISLDQGTWIIT
                     SYAVAEAICVPLTGWLADRFGSVRVFTVAMMAFGLFSLVCGLSPTLGV"
     gene            complement(349..1512)
                     /locus_tag="VN627_12325"
     CDS             complement(349..1512)
                     /locus_tag="VN627_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXD04082.1"
                     /translation="MADAAPAVPVDTTAGDDAAEAAFRRSQARRIWLVRLGIAVVVVG
                     VLWGGWYLLFGRNHVSTDNAYVNAEMAQVTPLVPGTVLGVKVSDTQAVKQGDVLVVLD
                     PATPQINLAQAEADLAAARRRFRQAQATAGAASAQAGASGADIGRARAQLASAQADFD
                     KARTDYTRRQSLSASGAVSGEELTTARRSFEAAQAGLASARAGVAQAEAAEKAAQGQY
                     QASAAVVAGSTEDTDPAVRAAVARVQQAQLDVKNTVIRAPIDGVVTQRKVQIGQRVAQ
                     GTPIMTIVPVGQLYIDANFKERQLRRVRIGMPAEVTADIYGGDVVYHGKVVGIAGGTG
                     SSMALIPAQNATGNWIKVVQRLPVRIALDPKELAAHPLRVGLSTEVEIDLTGS"
     gene            complement(1505..3028)
                     /locus_tag="VN627_12330"
     CDS             complement(1505..3028)
                     /locus_tag="VN627_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HXD04083.1"
                     /translation="MTANRQPDLLCCHRSRAFRRVPALLVAPALLLAGCAAVPKLGPR
                     SEPLAPAQIAAAQSLAGTPGAQWPTEGWWQAYGDPQLDSLVAEGLAKAPDVAAAEARL
                     ARAAALAQQAGAAQYPRLDVEGRTGEQRSSTNIGISDPRIQSLFPTGWRDAGQLSANL
                     GFDLDLWGKNRAALAAATSERRAAALDAQQARLVLAVAITSAYVDLARLEAERQVRAG
                     ELATATQSRKLFGERQANGLETRGAVAVSEADLAQARIGLSAAEEAVALRRNQLAALV
                     GAGPDRGLAITAPQLAVPAARAWPDDVTTDLVGRRPDIAAARERVEAAASRIKVARAD
                     FFPALKLSAMYGLQSVGLDLLFNKNSQTGFVGPAFSLPIFHGGELSGRYRGARADYDA
                     AVAAYNGAVLTGYREAADAITTARLVGQRLADARAARDAADLAFREINLRYRAGLVTF
                     LDVLQVQDRLLRAQLSVAQYEQAARNADVGLVRALGGGFAPTAAASATASMKDSAHG"
     gene            3117..3767
                     /locus_tag="VN627_12335"
     CDS             3117..3767
                     /locus_tag="VN627_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXD04084.1"
                     /translation="MDAASDQSCCPPLSRREARREERREAILDVAGRYFLEHGYAGAT
                     MSGIAAALGGSKATLWNYYGSKELLFADALERATREFREQLSLALNPDEGVAAALGSF
                     CSRYLDRLTNPEGIALYRLVMGEVVRFPEVGRIFYERAPLGVHQLLADFLEQAMARGE
                     LRRADSFDAAQFLTALCMARSHTKLLTGVTPLLTHAQAQADARAAIDVFLRAYAPD"
     gene            3876..>4861
                     /gene="bioB"
                     /locus_tag="VN627_12340"
     CDS             3876..>4861
                     /gene="bioB"
                     /locus_tag="VN627_12340"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HXD04085.1"
                     /translation="MTSTETIARPAAAAQAGGVPAVRTDWTRAEIAQLFALPFTELVF
                     RAAEVHRAHQAAGEVQLATLLSIKTGGCPEDCGYCAQSSSAEAGVKATKLMDVREVLQ
                     NAAQAKDNGSQRFCMGAAWRNPKDRDMPAIVEIVKGVRALGMETCMTLGMLTPAQAEQ
                     LAEAGLDYYNHNIDTSPERYGEVITTRTFHDRLETIANVRAAGINVCTGGIVGMGETR
                     EDRVGFIHALATMEQHPESVPVNALVPVKGTVLGDMLADTPLAKIDDIEFVRTVAVAR
                     ITMPLSMVRLSAGRESMSEATQALCFVAGANSMFTGDKLLTTPNAGDDADAA"
BASE COUNT          788 a         1630 c         1719 g          724 t
ORIGIN      
        1 cacgcccagg gtgggcgaga ggccgcagac cagggaaaac aggccaaacg ccatcatggc
       61 cacggtgaag acccgaaccg atccgaaccg gtcggcgagc cagccggtca agggtacgca
      121 gatggcctcg gccacagcat aggaggtgat aatccaggtg ccctgatcga gcgagatgcc
      181 aagattgccg gcaatgtgcg gcacggcgac attggcgatc gtgagatcga ggatcaccat
      241 gaagttgctg agcgccatga tgaacccggc gatcatctgg acgcggttcg acagcggcca
      301 actggtcggc acgccctccc cgggggggct cgcagtggta gccacggctt agctgccggt
      361 cagatcgatc tcgacctcgg tggacagacc cacgcgcagc gggtgcgcgg ccagttcctt
      421 ggggtcgagc gcgatccgga ccggcaagcg ctggaccacc ttgatccagt tgccggtcgc
      481 attttgcgcc ggaatgagcg ccatcgacga cccggtaccg ccggcgatgc cgacgacctt
      541 gccgtgatag acgacgtctc ccccatagat atctgccgtg acttccgccg gcatgccgat
      601 gcgaacccgg cgcagttggc gctccttgaa gttcgcatcg atgtaaagct ggcccacggg
      661 cacgatcgtc atgatcggag tgccctgggc gacgcgctgg ccgatctgga ccttgcgctg
      721 ggtgaccacg ccatcgatcg gggcgcggat cacggtgttc ttcacgtcaa gctgggcctg
      781 ctggacccgg gcgactgccg cgcggaccgc gggatcggta tcctcggttg acccggcgac
      841 gacggcggcg ctggcctggt actgcccctg cgctgccttc tccgcggctt cggcctgggc
      901 caccccggca cgggccgagg cgaggcccgc ctgggcggcc tcgaaggagc ggcgtgccgt
      961 ggtcagttct tcgcccgaga cagcgccgct ggccgaaagc gactggcggc gggtgtagtc
     1021 ggtacgggcc ttgtcgaaat cggcctgggc cgaggcgagc tgggcccggg cgcggccgat
     1081 gtcggctccg ctggcgccag cctgggccga tgcggcaccg gccgtggcct gggcctggcg
     1141 gaaccggcga cgggcggcgg cgagatcggc ttcggcctgg gcgaggttga tctgcggggt
     1201 cgccggatcg aggacgacga gcacgtcgcc ctgcttgacg gcctgggtat cgctgacctt
     1261 caccccaagc acggtgccgg gcaccagcgg ggtgacctgc gccatctccg cgttcacata
     1321 ggcattgtcg gtgctgacgt ggttgcggcc gaacagcagg taccagccgc cccagagcac
     1381 gccgacgacg accacggcga taccaaggcg gaccagccag atgcggcgcg cctgcgagcg
     1441 gcggaacgcc gcctcggcag cgtcgtcgcc ggcagtggtg tcgaccggta cggcaggggc
     1501 ggcatcagcc atgggcagaa tctttcatgc ttgcggtcgc actggcggcg gcggtcggcg
     1561 cgaacccgcc gccaagagcg cgaaccagcc cgacatcggc attgcgggcg gcctgctcat
     1621 actgggcgac ggacagctgc gcgcgcagca gccggtcctg gacctgaagg acatcgagga
     1681 aggtgacaag ccccgcgcga taacgcaggt tgatttcgcg gaaggcgaga tcggccgcat
     1741 cgcgggcggc gcgggcatct gccagccgct ggcccaccag gcgggccgtg gtgatggcat
     1801 cggctgcctc gcggtagccg gtgagaaccg caccgttgta ggccgcgacc gctgcgtcgt
     1861 aatccgcccg ggcgccgcga taacgccccg acagctcgcc gccgtggaag atcggcaggc
     1921 tgaaggccgg gccgacgaat ccggtctgcg aattcttgtt gaacaggaga tcgaggccga
     1981 ccgactggag cccgtacatc gcggaaagct tgagcgcggg gaagaaatcg gcgcgggcaa
     2041 ccttgatccg gctggccgcc gcctcgaccc gctcgcgcgc ggcggcgata tccgggcggc
     2101 ggccgacgag atcggtcgtc acatcatcgg gccaggcgcg ggccgccggc acggccagtt
     2161 gcggcgcggt gatggcgaga ccgcggtccg gcccggcgcc gaccagcgcg gccagctggt
     2221 tgcggcgcag cgccacggct tcctcagccg cgctgaggcc gatccgggcc tgggccaggt
     2281 cggcctcgga gacagcgact gcgccgcgcg tttcaaggcc attggcctgc cgctcgccaa
     2341 acagcttgcg cgattgggtg gccgttgcca gctctccggc gcgcacttgg cgctccgcct
     2401 cgagccgggc gagatcgacg taggccgaag tgatcgccac cgcgagaacc agccgggcct
     2461 gctgcgcatc gagcgcggcg gcgcggcgtt ccgacgtggc ggcggccagc gccgcgcggt
     2521 tcttgcccca cagatcgaga tcgaagccga ggttggccga cagctggccc gcatcgcgcc
     2581 agccagtggg aaacaggctc tgtatccgtg ggtcggagat accgatgttg gtgctgctgc
     2641 gctgttcccc ggtacgccct tcgacatcga gccggggata ttgcgcggcc ccggcctgct
     2701 gggcgagcgc cgcggcccgg gcaaggcgag cctcggccgc agccacgtcg ggtgccttgg
     2761 cgaggccttc cgcgaccagg ctgtcaagct ggggatcgcc gtaggcctgc caccagcctt
     2821 cggtgggcca ctgcgcgccc ggggtgccgg cgaggctttg cgcagcggcg atctgggcgg
     2881 gagccagcgg ttccgaccgg ggcccgagct tgggcacggc ggcacagccg gccagcagca
     2941 gcgccggcgc gacgagcaag gccgggacgc gccggaaagc acgcgagcgg tggcagcaaa
     3001 gaaggtcagg ctgtctgttg gcggtcatga aaagtgtact atccggtatg gattgtgcaa
     3061 atgcagcgcc gcgtcgccat tgtcaaccga aaaaccgtac tgtatggtac gcctagatgg
     3121 atgccgcgtc agaccaatcc tgttgtccgc cgctgagtcg gcgggaagcc cgccgtgagg
     3181 aacggcgcga ggcgattctc gacgtggccg ggcgctactt cctggagcac ggctatgccg
     3241 gggcgacaat gtccggaatc gccgcagccc ttggcggttc caaggcaacc ctgtggaatt
     3301 actatggctc caaggagttg ctgtttgccg acgctctgga acgggcgacg cgggagtttc
     3361 gggaacagct ctcgctcgca ctcaacccgg acgaaggggt agccgcggcg ctgggctcgt
     3421 tctgctcgcg ctatctcgac cggctgacca accccgaggg cattgcgctg taccgcctgg
     3481 taatggggga ggtcgtccgt ttccctgagg tcgggcgaat tttctacgag cgcgcgccgt
     3541 tgggcgtcca ccagttgctc gccgatttcc ttgagcaggc catggcgcgc ggggaacttc
     3601 gccgcgccga ttccttcgat gcggcacagt tcctgacggc gctgtgcatg gcccggtcgc
     3661 acaccaagct gctgaccggg gtaaccccgc tgctgaccca cgcccaggcc caggccgatg
     3721 cccgcgccgc gatcgacgtg ttcctgcgcg cctacgcgcc tgactgaacc cgcgcgcccc
     3781 ctgcaggccc ttgccccggg ccgcaaattc ggcaagggga gcccgcaaga tgcgcgcagc
     3841 gcgccgatgc tgccgccaag tggatccgtg agcccatgac cagcaccgaa accatcgccc
     3901 gtcccgcggc tgctgcccaa gccggtggcg tccccgccgt tcgtaccgac tggacccggg
     3961 ccgagatcgc ccagctgttc gccctgccct tcaccgagct ggtattccgc gccgccgagg
     4021 tgcaccgcgc tcaccaggcc gcgggcgagg tccagctggc gaccctgctc tcgatcaaga
     4081 cgggcggctg cccggaagat tgcggctatt gcgcccagtc gtccagcgcc gaggcgggcg
     4141 tcaaggcgac caagctgatg gacgtgcgcg aagtgctcca gaacgccgcc caggccaagg
     4201 acaacggcag ccagcgcttc tgcatgggcg cggcctggcg caacccgaag gaccgcgaca
     4261 tgcccgcgat cgtcgagatc gtgaaaggcg tgcgcgcgct cggcatggaa acgtgcatga
     4321 cactcggcat gctgaccccg gcccaggccg aacagctcgc cgaggccggc ctcgattact
     4381 acaaccacaa catcgacacc tcgcccgaac gctatggcga ggtgatcacc acccgcacgt
     4441 tccatgaccg gctggagacc atcgccaacg tccgcgcggc tgggatcaat gtctgcaccg
     4501 gcgggattgt cggcatgggc gagacgcgcg aggaccgggt cggcttcatt catgccctcg
     4561 ccacgatgga acagcacccg gagagcgtgc cggtgaatgc gctggttccg gtcaaaggca
     4621 ctgtgctggg cgacatgctg gccgatacgc cgctggccaa gatcgacgat atcgaatttg
     4681 tccgcacggt ggccgtggcg cggatcacca tgccgctctc gatggtgcgg ctctcggcgg
     4741 ggcgcgaatc gatgagcgag gcgacccagg cgctgtgctt cgttgcgggc gccaattcga
     4801 tgttcaccgg cgacaagctg ctgaccacgc ccaatgccgg cgacgatgcg gatgcggcga
     4861 t
//
LOCUS       DATNBV010000521         2337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3101028,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000521 DATNBV010000000
VERSION     DATNBV010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3101028"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..439
                     /locus_tag="VN627_12345"
     CDS             212..439
                     /locus_tag="VN627_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04086.1"
                     /translation="MTTLNPHAGPQPDPALALRLEHYAESHRATATSPATAAELDRYD
                     IAVVLQAIYEWGGYRYSNASDAIAAAKRSPK"
     gene            436..660
                     /locus_tag="VN627_12350"
     CDS             436..660
                     /locus_tag="VN627_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04087.1"
                     /translation="MSRALNLAMTEAEIVKHCAEKAIGISALEALPGGGVRLVCMSGH
                     GAAQIRAKLKRHLIAGEVRRERLRPARPLW"
     gene            complement(921..1136)
                     /locus_tag="VN627_12355"
     CDS             complement(921..1136)
                     /locus_tag="VN627_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04088.1"
                     /translation="MSGPVIRGTNLQFFLARAAQAREEAEAATLDHVRERCRRSEAAW
                     SALADKAQRSERLRVEEQIRKAEAGPK"
     gene            complement(1198..1404)
                     /locus_tag="VN627_12360"
                     /pseudo
     CDS             complement(1198..1404)
                     /locus_tag="VN627_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517701.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
     gene            complement(1620..1832)
                     /locus_tag="VN627_12365"
     CDS             complement(1620..1832)
                     /locus_tag="VN627_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04089.1"
                     /translation="MGYIIGGILGIIIAIVLIIAGIKGGPIAITNRNTGQTGIMTDTP
                     NWLLIIIGIVVGLGSIGSIIGGATGH"
     gene            complement(1875..2069)
                     /locus_tag="VN627_12370"
     CDS             complement(1875..2069)
                     /locus_tag="VN627_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04090.1"
                     /translation="MYIVGGIIGILIGLAFIYAGFKGGTLWVEKDGQRAPVSGQPNWV
                     MIIIGLVAAGSGIASLIKGS"
     gene            complement(2092..>2337)
                     /locus_tag="VN627_12375"
     CDS             complement(2092..>2337)
                     /locus_tag="VN627_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04091.1"
                     /translation="PGNWGVERMAETTAETNRNVGIGLIVGGLLLAGLLVVLIVVGWT
                     STPRNLILVVAASFGQLLAVYLIGYGIKKLAGAGSAA"
BASE COUNT          371 a          800 c          764 g          402 t
ORIGIN      
        1 gcgccgccca cgtgaaaata tcgctcgggc caaatccaac agtcgcgcgc gcgtggcgac
       61 cgtgaacttc tgtgagcttt cgcccgaata tccgggcctt ctcgcgccac ccgtgctaca
      121 ggcggcgcat catcggcatt ctcctccgca ggcgccgatg gctgagagtc ccccaaatgc
      181 tcccgcttgc tgcgcaggag cgctccgtaa aatgactacc ctcaaccccc acgccggccc
      241 gcagccggac ccggcgctcg cgctccggct cgagcattat gccgagtccc accgggcgac
      301 cgcgacctcg cccgcgaccg ccgccgagct cgaccgctac gacatcgccg tggtgctcca
      361 ggcgatctac gaatggggcg gctaccgcta ctccaacgcc tcagacgcga tcgccgccgc
      421 caaaaggagc ccgaaatgag ccgcgccctg aacctcgcca tgaccgaggc cgaaatcgtc
      481 aagcattgcg ccgagaaggc catcggcatt tcggcgctgg aggcgctgcc cggcggcggc
      541 gtccgcctgg tgtgcatgag cggtcacggg gcggcgcaga tccgcgccaa gctgaagcgc
      601 cacctgatcg ccggcgaggt gcggcgcgag cggctgcgcc cggcgcggcc gctgtggtag
      661 cgcgggcgcg cctcagttcg agctaggcgg tcccggcgcg aggacgaaca ggctgaaaaa
      721 gcaacgtcga gtgtcgggac cgcgtctctg gaccggaaaa gcttagtgcg gacggctcgg
      781 cggagttgcg gggctggccc ggcggaatga ctgccgccgc gtgcgccggc cgccctcgat
      841 ctggcgccac aggttcaggg tcttgtcgtc catgcgatcc gccgaacgcc ggcgtgcacg
      901 ccgggttcca gcagccgcag tcacttgggc ccggcttcgg cctttcgaat ctgctcttcg
      961 acgcgcaggc gctcgctgcg ctgggccttg tcggccagcg cgctccacgc cgcctcggag
     1021 cggcggcaac gctcgcgcac atggtcgagc gtcgccgcct ccgcctcctc ccgcgcctgg
     1081 gcggcgcggg cgaggaagaa ttgcaagttg gtgccccgga tgaccgggcc ggacaaatta
     1141 ctcgccctgc tggtcggtcg caggctcttc ggcgcttgct tcgggctggg tcgaatcttc
     1201 ggccgccttg aggttggcgg cagccatctt gccgcgattg tcctgctgca gctcgtagct
     1261 gacgcgctgg ttctcgcgca gcgtctgcat gcccgagcgc tcgacggcgc tgatgtggac
     1321 gaaggcgtcc tggccgccgc cgtcgggctg gatgaagccg tagcccttgg tctcgttgaa
     1381 gaatttgact gtgccgactg gcatttctct gactttccct tctggggcgg gccgagtgcc
     1441 ggtccgcttc gagctaggag cgcacgagaa ggaaaaagga gccgcaaagc gcctgtagac
     1501 cgtcattttg cgactagtag ccacgcctgg atacgcgcgc tggcccgaaa ttgtaaccct
     1561 cggcacgata tcgcgctcat gcctccgcca gaccggtcgg agcgggcagc ggaccggggt
     1621 cagtggccgg tggcgccgcc gatgatgctg ccgatgctcc cgaggccgac gacaatcccg
     1681 atgatgatca gcagccagtt gggggtgtcg gtcatgattc cggtctggcc ggtgttgcga
     1741 ttggtgatgg cgatcggccc gcccttgatc ccggcgatta tgagcacgat ggcgatgatg
     1801 atcccgagaa ttccgccgat gatatagccc atgattattc tccctctgcc cctacgcggc
     1861 cgagcgccgc cgacctagct gcccttgatg aggctggcga ttccggagcc tgccgcgacc
     1921 agcccgatga tgatcatcac ccagttcggc tggccgctga ccggcgcccg ctgcccgtcc
     1981 ttctcgaccc acagggtgcc gcccttgaag cccgcgtaaa tgaacgcgag accgatcagg
     2041 atgccgatga ttccgccgac aatgtacatt gcgttcctcc ctagcgcgct gtcacgccgc
     2101 gcttcccgcg ccggcgagct tcttgattcc gtagccgatc agatagaccg ccaggagctg
     2161 gccgaacgaa gcggcgacga ccaggatgag gttgcgcggc gtgctggtcc atccgacgac
     2221 gataagcacg accaacaatc cggcgaggag gaggccgcca acgatcagtc cgatgccgac
     2281 gttgcggttc gtttcagcgg ttgtttcggc catgcgctcg accccccagt tgcctgg
//
LOCUS       DATNBV010000522         2286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_598553,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000522 DATNBV010000000
VERSION     DATNBV010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598553"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1557)
                     /locus_tag="VN627_12380"
     CDS             complement(31..1557)
                     /locus_tag="VN627_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXD04092.1"
                     /translation="MSRVAQAAAAAALLAFALSAPAVQAAPRPAAKAAGRIQPASKAD
                     LALQADLLAHIKVLASDEFGGREPGTESEAKTLRYLGQQWFDIGLVSGTNDPGHPWFA
                     PVSLVAREPAGSAAQFARKGRRIYLPQGSVLMLTSGSRALVENAPLLFVGKASQADQL
                     ARSELAGRVVVLLDAGERDSDRQNALLKAGASAVLTVLDDGRSLAQVTARRKRSGYAL
                     ASDALGGDLEGFIDPASFDRLATVAGTSLAALQAAAGDPAFVPRALDLSATLEATTRE
                     TTIRTHNLIGKLEGRRPDQGAVLFMAHWDHFGQCAEPPAEHLICNGAIDNASGVAALT
                     EVARQLSRMPQPDRDIYFVATTAEELGLLGAEAFAENPPLPLKQVVAAFNIDTVAIAP
                     AGTPFAIVGKGMTRLDPQIAAAAKAFRRPLAAGDEANAYIKRQDGWALLQHDVPAVMV
                     TTAYGDLKRLEAFFDSDYHRPTDVVKPGIELGGAAQDVGFLVQLGRWFADPKRVPLVK
                     "
     gene            complement(1738..>2286)
                     /locus_tag="VN627_12385"
     CDS             complement(1738..>2286)
                     /locus_tag="VN627_12385"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HXD04093.1"
                     /translation="EAAGPVDVLVNNAGMQFVAPVDEFPVEKWDQIIALNLTAAFDAC
                     RLAIPHMKAQNWGRIINIASAHSLTASPFKAAYVAAKHGLAGLTKTIALELATTKITA
                     NCISPGYVWTPLVENQIPDTMKARGMTREQVISEVILERQPTKDFARPEDLGEMAVFL
                     CRDAAQQITGTNISMDGGWTAQ"
BASE COUNT          359 a          793 c          762 g          372 t
ORIGIN      
        1 cctgttcttc tccgcgcctc cgcgtgaacc ctacttcacc agcggcaccc gcttcggatc
       61 ggcgaaccac cgcccgagct ggaccaggaa gcccacgtcc tgcgccgccc cgcccagctc
      121 gatgcccggc ttcaccacgt cggtgggccg gtggtagtcg ctgtcgaaga aggcctcgag
      181 ccgcttgaga tcgccatagg ccgtggtcac catgacggcc ggaacatcat gctggagcag
      241 ggcccagccg tcctgccgct tgatataggc attggcctcg tcgccagctg ccagcgggcg
      301 ccggaatgcc ttggcagccg ccgcgatctg gggatcgagc cgggtcatgc ccttgccgac
      361 gatcgcaaac ggcgtgccgg ccggggcgat ggccacggta tcgatattga aggcggcgac
      421 cacctgcttg agcggcagcg gcgggttctc ggcaaaggcc tcggcgccga gcaggccaag
      481 ttcctccgcc gtggtcgcca cgaaatagat gtcgcggtcg ggctgaggca tgcggctgag
      541 ctggcgcgcc acttccgtca gggcggcaac gccgctggca ttgtcgatgg cgccattgca
      601 gatcagatgc tccgccggcg gttcggcgca ttgcccgaaa tgatcccagt gcgccatgaa
      661 cagcaccgcg ccctggtcgg ggcggcggcc ttccagtttg ccgatcaggt tgtgggtgcg
      721 gatcgtggtt tcgcgggtag tcgcttccaa ggtcgcagac aggtccaggg cgcgcggcac
      781 gaaggccgga tcgccggcgg cagcctgcag agcggccagg ctggtgccgg ccaccgttgc
      841 gagccggtcg aacgaggcgg gatcgatgaa gccttcaaga tcgccgccca gcgcgtcgct
      901 cgccaaggca tagcccgacc gcttgcggcg cgccgtcacc tgggcgaggc tgcggccgtc
      961 gtcgagcacg gtcagcacgg ccgaggctcc ggccttgagc agcgcgttct gccggtcgct
     1021 gtcgcgctcc ccggcatcga gcaggacgac cacccgcccc gccagctcac ttcgggccag
     1081 ctggtcggcc tggctggcct tgcccacgaa cagcagcggc gcattttcca ccaaggcgcg
     1141 cgaacctgag gtgagcatga ggaccgagcc ctgcggcagg tagatacgcc ggcccttgcg
     1201 ggcaaactgc gccgccgaac ccgccggctc gcgcgccacc agcgaaacgg gcgcgaacca
     1261 cgggtggccg ggatcgttgg tgccggaaac caggccgatg tcgaaccatt gctggccgag
     1321 atagcgcagg gtcttcgcct cgctctcggt gcccggttcg cggccgccga attcgtcgct
     1381 cgccagcacc ttgatgtgcg ccagcagatc ggcctgaagg gcaaggtcgg ccttcgaggc
     1441 tggctgaatc ctgcccgccg ccttggcggc cgggcgcggc gctgcctgta ctgccggagc
     1501 cgagagggca aacgccagca aagccgccgc agcagccgcc tgagccactc ggctcatcgc
     1561 tcccatgcct cgtctcatcg ccaccacttg tgaacgacaa cgcacgggca aacaagaagc
     1621 ctccactcgg ttcagcgaaa tttacgcggt gtggcgcaaa agacgcggca cggccggaat
     1681 ctcgccgtcg ccgcgcctcc actcacagta aaggtggccc ttccgctggt cccgcggtca
     1741 ctgcgccgtc cacccgccat ccatcgagat gttggtcccg gtgatctgct gcgccgcatc
     1801 ccggcacagg aacacggcca tctcgccaag gtcttcgggc ctggcgaaat ccttggtcgg
     1861 ctggcgctcg aggatcacct cgctgatcac ctgctcgcgc gtcatgcccc gggccttcat
     1921 cgtgtcgggg atctggttct ccaccagcgg ggtccagaca tagcccgggc tgatgcagtt
     1981 ggcggtgatc ttggtggtcg ccagttcgag ggcaatggtc ttggtcagcc cggccagccc
     2041 gtgcttcgcc gcgacatagg ccgccttgaa cggcgaggcg gtaagcgaat gggcgctggc
     2101 gatgttgatg atgcggcccc agttctgcgc cttcatgtgg gggatcgcca gccggcaggc
     2161 gtcgaacgca gccgtcaggt tcagcgcgat gatctggtcc cacttttcga cgggaaactc
     2221 gtccaccggc gcgacgaact gcatgccggc attgttcacc agcacgtcga ccggccccgc
     2281 ggcctc
//
LOCUS       DATNBV010000523         2523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2496172,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000523 DATNBV010000000
VERSION     DATNBV010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496172"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VN627_12390"
     CDS             complement(<1..1274)
                     /locus_tag="VN627_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HXD04094.1"
                     /translation="MKFNFNNPYPTTRLPVLARNIVSTSHPLAAQAGLRIMWKGGNAV
                     DAAIAAAAAMTICEPLSNGLGSDAFAIVWDGQQLHGLNASGSAPAAWTPEHFKRKYGA
                     EAVRPPKRGFDSVTVPGAVAGWVALSERFGKLPFADLMGPAIEIAERGYLLPVVVQQK
                     WAAATPELRDLPGFAQCFLPWGRAPEVGELFQFKAAARGLRAIGETKGKAYYGGEIAQ
                     AIEKFAATNGGSIAARDFAAYQPEWVKPIGKDYRGYTLHEIPPNGQGIAALIALGILQ
                     NFDVAELPVDGADSQHLQIEAIKLAFADVYKYVAEPSSMTVTPEQMLDDRYLAGRARL
                     IDMKRAQDFGAGNPVKGGTIYLTAADENGMMVSFIQSNYMGFGSGCVEPTFGISLQNR
                     GHGFSLDAGPNQVAPRKRPFHTIIPAFLTKNG"
     gene            complement(1360..>2523)
                     /locus_tag="VN627_12395"
     CDS             complement(1360..>2523)
                     /locus_tag="VN627_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04095.1"
                     /translation="TYFSVHNKKYDIIVSEPSNPWVSGVAGLFSEEFYRQVKQHLNRD
                     GVFAQWVQLYEIDTDLVFSILKAVSDNFPDFAIYASNDQDMIIIAKNQGTLPAIDTQV
                     LGNREISAAMNRIGIRGLQDLEARKIGNKRLFERLLAASGVRANSDYYPVVDQNAART
                     RFLRANAFVIRELSELPLPALEMLTGSRPSWNTTDVARSRLFSKSQDVGTAMMLRDYG
                     LFGQFDPVHGTVPPDIQQLALQFGQIFLACRPEANQGARQIALYNTLIRTAPYLRPQE
                     MEPIWNRLEASPCARSLTLPERNWIALFKAVSRRDAAAMVSGARAILESGQRMPAEVV
                     EFVVASSMLGSLTLGDRQDAYRLWSAYGPKLFKDGRPSLLYRILVAESAMADR"
BASE COUNT          453 a          777 c          792 g          501 t
ORIGIN      
        1 tcgccgtttt tcgtcaggaa cgccgggatg atcgtgtgaa acggccgctt gcggggcgcg
       61 acctgattcg gtcccgcgtc caggctgaag ccgtggccgc ggttttgcag gctgatgccg
      121 aaagtgggct cgacgcagcc cgagccgaac cccatgtagt tactctggat gaagctgacc
      181 atcatgccgt tctcgtcggc ggcggtgaga tagatcgtgc cgcccttgac cgggttgccg
      241 gcgccgaagt cctgcgcgcg cttcatgtcg atcagcctgg cccggccggc cagatagcgg
      301 tcgtccagca tctgctcggg cgtcaccgtc atgctcgaag gctcggccac atatttgtag
      361 acatcggcaa aagccagctt gatcgcttcg atctgcaggt gctgcgagtc ggcgccgtcc
      421 accggcaact cggcgacgtc gaagttctgc aggatgccca gcgcgatcag cgccgcgatg
      481 ccctggccgt tgggcgggat ctcatgcagc gtgtagccgc gatagtcctt gccgatcggc
      541 ttgacccatt cgggctggta ggccgcgaag tccctggcgg cgatgctgcc gccgttcgtc
      601 gctgcgaatt tctcgatggc ctgggcgatc tcgccgccgt agtaggcctt gcctttggtc
      661 tcaccaatcg cacgcagtcc gcgtgccgcc gccttgaact ggaacagctc gcccacttcg
      721 ggcgcgcggc cccacggcag gaaacactgc gcaaagcccg gcaggtcgcg cagctcgggt
      781 gtggcggcag cccatttctg ctgcaccacg accggcagca gatagccgcg ctcggcgatt
      841 tcgatggccg ggcccatcag gtccgcgaaa ggcagcttgc cgaagcgctc gctcagcgcg
      901 acccagccgg cgacagcgcc gggcaccgta accgaatcga agccgcgctt gggtggcctg
      961 acagcctcgg ccccgtactt gcgcttgaag tgctcgggcg tccacgcggc gggcgcgctg
     1021 cccgaagcgt tcaggccgtg cagctgctgg ccgtcccaca cgatcgcgaa tgcgtcgctg
     1081 cccaggccgt tgctcaacgg ctcgcagatc gtcatggcgg cggcggcggc aatcgcggca
     1141 tcgacggcgt tgccgccctt ccacatgatg cgcagccccg cctgcgcggc cagcgggtgc
     1201 gaagtggaaa cgatgttgcg agcgagcacc ggcaggcgcg tggtcgggta cgggttgttg
     1261 aaattgaact tcatgtaccg attatggtgg gctggtccgc atcttgcatg tcacggccga
     1321 ggagttccgc atgcaggaca atgcgaacag ccaccgctat taccgatcgg ccatggcgct
     1381 ttccgcaacc aatatgcgat aaagcaagct gggtcggccg tccttgaata acttggggcc
     1441 gtatgcgctc cagagcctat aggcatcttg ccgatcacct agcgtcaaac tgcccagcat
     1501 gcttgacgcg accacgaatt ccaccacctc ggcaggcatg cgctggccgc tctccaatat
     1561 ggccctggcg ccgctcacca tggctgcggc atcccgcctg cttacggcct tgaacaatgc
     1621 gatccagttc ctctcaggca atgtcagaga acgggcgcat gggctagctt ccagcctgtt
     1681 ccagatgggc tccatctcct gtggtctcag gtagggggcc gtcctgatca gtgtgttgta
     1741 aagcgcgatc tgcctggcgc cttgatttgc ttcagggcgg cacgcaagga agatctgccc
     1801 gaactgcaaa gccagttgct gaatatccgg aggcacagtc ccatgcaccg ggtcgaattg
     1861 cccgaataga ccgtaatcgc ggagcatcat cgccgtcccg acatcctgcg atttggagaa
     1921 cagacgggac cgggcgacgt cggtcgtgtt ccaggacgga cgcgagcctg tcagcatttc
     1981 cagcgccggt agcggaagtt ctgaaagttc ccgaatcaca aatgcgtttg cccgcaaaaa
     2041 acgggtgcgc gcggcatttt gatcgacaac cgggtaatag tcggaattgg cacggacgcc
     2101 ggaggcagcg agcagtcttt cgaacagacg tttattgccg atctttcttg cttcgaggtc
     2161 ctgcaggccg cgaatgccga tcctgttcat ggccgccgat atctcccggt tgcccaggac
     2221 ttgggtatcg atggcaggca gggttccctg gttctttgcg atgatgatca tgtcctggtc
     2281 gttgcttgcg taaatcgcga agtcgggaaa gttatcggaa acagccttga ggatggagaa
     2341 aaccaggtcg gtatctatct cgtacagctg tacccactgg gcgaacacgc cgtcccggtt
     2401 caggtgctgt ttgacctggc ggtagaactc ttccgaaaat agccccgcta caccgctgac
     2461 ccacgggttg gacggctcgg atacgatgat gtcgtacttc ttgttgtgga cggagaagta
     2521 ggt
//
LOCUS       DATNBV010000524         6129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2851671,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000524 DATNBV010000000
VERSION     DATNBV010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2851671"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VN627_12400"
     CDS             complement(<1..386)
                     /locus_tag="VN627_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXD04096.1"
                     /translation="MRNVAASTGEGLRRRVRLAREPRPAGVRTPPLGLLLRELALLVN
                     RRRGGALPVSLAADPLPVMILPGFGAHPARMRRMGAALEEAGHLVFDWGLGFNLGPTP
                     ENFAFLLARVRRIAGATGRPVALVGW"
     gene            complement(477..1046)
                     /locus_tag="VN627_12405"
     CDS             complement(477..1046)
                     /locus_tag="VN627_12405"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HXD04097.1"
                     /translation="MRRIGMMVGAGLLAALAVTGCKKESAPGQPKSIAEVAQQAAGMP
                     KPLPGLYRSTAELVSLEAPGLPAGAAAQMKQMMASRAAPRDFCLTGAEADKGYEERVK
                     KLAGRPDCHFDHYSAVAGKLDAQLTCTGGGNGAQGAVRSVLTMQGTMAPDGSDVTLAM
                     AQSGAQLPGGGMKMTMHVKSARVGDCPAS"
     gene            complement(1124..1492)
                     /locus_tag="VN627_12410"
     CDS             complement(1124..1492)
                     /locus_tag="VN627_12410"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXD04098.1"
                     /translation="MTTDAADRNEIEERGARRLALAMPARCRMLSGFTEDVVIRDVSE
                     SGCRVAGHALHLRIGDRVLLRPPGMESLPGVVRWCERGEAGIEFERGLHVAVVEHLHR
                     RYVTFLAAEVPYRAPRRLAA"
     gene            1652..3850
                     /gene="uvrB"
                     /locus_tag="VN627_12415"
     CDS             1652..3850
                     /gene="uvrB"
                     /locus_tag="VN627_12415"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HXD04099.1"
                     /translation="MSELVIRRGLAEPDTSGNFIPHKPQRPEKSDGGRPFRIVSDYEP
                     AGDQPGAIADLVATALEGEQTQVLLGVTGSGKTFTMAKVIEQLQRPALILAPNKILAA
                     QLYGEFKSFFPDNAVEYFVSYYDYYQPEAYVPRSDTYIEKESSVNEAIDRMRHSATRA
                     LLERDDVIIVASVSCLYGIGSVETYSAMIFDLKKGDTQDQREIIRKLVALQYKRNDAA
                     FARGNFRVRGDNLEIFPSHYEDMAWRISFFGDEIEEIAEFDPLTGKKGASLDKVRVYA
                     NSHYVTPGPTMKQATEAIRFELAERLKELEAEGKLLEHQRLEQRTNFDLEMIAATGSC
                     AGIENYSRFLTGRLPGEPPPTLFEYLPDNALLFVDESHQTVPQIGAMSKGDHRRKITL
                     AEYGFRLPSCIDNRPLRFNEWDAMRPQTVAVSATPGPWEMEQAGGVFAEQVIRPTGLI
                     DPPVEIKPVEDQVQDCIEECRKTAKLGYRTLVTTLTKRMAEDLTEFMHEAGLRVRYMH
                     SDVETLERIELIRDLRLGVYDVLVGINLLREGLDIPECGLVCILDADKEGFLRSETSL
                     IQTIGRAARNVDGRVILYADRMTGSMERAIAETDRRRAKQDAYNREHGITPATIKRNI
                     HDIVADTASRDGVVIDLEDDARNNLVGHNLRAYIEELEQRMRAAAADLEFEEAGRLRD
                     EIRRLEAEELGLPEAEHAGGSRAPRVGRSNEGKPGTRKTRYGKTQRKWGR"
     gene            complement(3872..4282)
                     /locus_tag="VN627_12420"
     CDS             complement(3872..4282)
                     /locus_tag="VN627_12420"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXD04100.1"
                     /translation="MPVSTDDYVAVADHIARYCWKVDAGDADGWADLWLEDGVFTGIS
                     PEPIVGREALRQVPIGIKASVGYTMAHKGVNIFCDYGESKDVIKAHLYNFVTTWMSGE
                     PGKPNVMARCVMTLVRTGGGWKIKSNEADLLTGA"
     gene            4398..4985
                     /locus_tag="VN627_12425"
     CDS             4398..4985
                     /locus_tag="VN627_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04101.1"
                     /translation="MSVVRSFTTTSMLVGGILALSLGGCSQSQSRELVDAAPGGGYPA
                     RLALAELAPADDAAPLQPDGAGWARKGDEVTFGVAGQPALLSLACTHDAAGAASLALA
                     RHTRAEAGAKALLALIGNGRIARLPLDVVTPGEAGEWRGSIPAADGRLDVLRGGNRIE
                     GTLPGGGTLLMPASGEPGRLLEECRASDRAAAGAV"
     gene            complement(5067..5624)
                     /locus_tag="VN627_12430"
     CDS             complement(5067..5624)
                     /locus_tag="VN627_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04102.1"
                     /translation="MQQLEQNWPILAIVVVLALLVAWWLMSRAARPVQRSHRPDVLDE
                     GVGPAERNQALIDAAPAASAAYAIPPAAGTLGGVAEVIAAAAQEEAADAGAAETTPGL
                     PEQQADDLTRIKGLGPKLATVLGQLGVTSYAQVAGWTDADLARIDDQLPPAFKGRPAR
                     DKWVEQARFLAAGDTAGYEGQFGKL"
     gene            complement(5718..>6129)
                     /locus_tag="VN627_12435"
     CDS             complement(5718..>6129)
                     /locus_tag="VN627_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HXD04103.1"
                     /translation="YVARYQNFVLSLFTIVLQGLLTVGGIMLVRASGHAEGWQIAAAA
                     AALMIALATASLLKARLLARVLGEPINNFRGALAWAVVPAVCVGAAARTFLPEWAMIA
                     IGIPAILGIYVWMLWHKGFGPDDRVLFRKEVAPA"
BASE COUNT         1021 a         2059 c         2143 g          906 t
ORIGIN      
        1 ctccacccga cgagcgcgac cggccgcccc gtggcccccg cgatgcggcg cacccgggcg
       61 aggaggaagg cgaagttttc aggggtaggc ccgaggttga agcccaagcc ccaatcgaac
      121 acgaggtgtc ccgcctcctc gagcgccgcg cccatgcgcc gcatgcgcgc cgggtgggcg
      181 ccgaagccgg gaaggatcat taccggcagg ggatcggccg cgaggctgac cggcaaggct
      241 ccgccccggc ggcgattcac cagcagggcg agttcgcgca ggaggaggcc gagcggcggg
      301 gtgcgcacgc cggcggggcg cggctcgcgc gccaggcgca cccggcggcg caggccttcg
      361 cccgtgctgg ccgcgacgtt acgcatgagc gacagagtca ccatcggcgt gaattgcccc
      421 gctgtgcctg tttgcaggct gaacggggcg gcagagccga aagttccgga ggccgctcag
      481 ctcgccgggc aatcgccgac ccgggctgac ttgacgtgca tggtcatctt catgccgccg
      541 ccgggcagct gggccccgct ctgcgccatg gccagggtga cgtcggagcc atcgggggcc
      601 atcgtgccct gcatggtgag gaccgagcgc acggcgcctt gcgcgccatt gccgccgccc
      661 gtgcaggtca gctgcgcatc gagcttgccg gcaacggcgg agtagtgatc gaaatggcaa
      721 tcgggccggc cggcgagctt cttcacccgc tcctcgtagc ccttgtcggc ctcggccccg
      781 gtcaggcaaa aatcgcgcgg ggcggcgcgg ctggccatca tctgcttcat ctgcgcggcg
      841 gccccggcgg gcaggcccgg cgcctcgagg ctgaccagtt cggcggtgga gcgatagagc
      901 cccggcaggg gcttgggcat gccggcggcc tgctgggcca cttcggcaat gctcttgggc
      961 tggcccggcg ccgattcctt cttgcacccc gtcacggcga gggcggcaag cagccccgct
     1021 cccaccatca ttccgatcct gcgcatggcc cgctccctcc gtggctaccc gggaaggcta
     1081 ccagcgccgg gcgcttgtgc caatcggttc aggcccccga gagtcaggcg gcgaggcgac
     1141 ggggcgcgcg gtagggcact tcggcggcga ggaaggtgac gtaccggcga tggaggtgtt
     1201 cgaccacggc gacgtgaagc ccccgctcga attcgatccc cgcctcgccc cgttcgcacc
     1261 agcgcacgac gccgggcagg ctttccatgc ccggcgggcg gagcaggacg cgatcgccga
     1321 tgcgcaggtg cagggcatgg ccggcgacgc ggcagccgct ttccgacacg tcgcggatca
     1381 ccacgtcttc ggtaaagccc gaaagcatcc ggcagcgcgc gggcatggcc agcgccaacc
     1441 ggcgggcgcc gcgttcctcg atctcgttac ggtcggccgc atcggtggtc atgacgcata
     1501 ctccagccac ttgtcgcaag gccaagaacg gcaccggcag gggcgttttc accccatgcc
     1561 atgaacgggc ccaagatatg gttaaggcgt gcttgaccat gaccacgggg acattgccga
     1621 atgcccctgc cgcgcccata ttgccggttc catgagcgaa ctcgtcatcc gccgcgggct
     1681 ggccgagccc gatacttccg gcaacttcat cccccacaag ccgcagcggc ccgagaagtc
     1741 ggacggcggg cggccgtttc gcatcgtctc cgactatgaa ccggcgggcg accagcctgg
     1801 cgccatcgcc gacctggtgg caaccgcgct ggagggcgag cagacccagg tcctgcttgg
     1861 cgtcaccggc tcgggcaaga ccttcaccat ggccaaggtg atcgagcagt tgcagcgccc
     1921 ggccctgatc ctggcgccga acaagatcct tgcggcccag ctctacggcg agttcaagag
     1981 cttcttcccg gacaacgcgg tcgagtattt cgtatcctac tacgactact accagcccga
     2041 ggcctacgtg ccgcggtcgg acacctacat cgagaaggaa agctcggtta acgaggcgat
     2101 cgaccggatg cgccactcgg ccacgcgggc gctgctggag cgggacgacg tgatcatcgt
     2161 cgcttcggtg agctgtctct acgggatcgg ttcggtcgag acctattcgg cgatgatctt
     2221 cgacttgaag aagggcgaca cgcaggacca gcgcgaaatc atccgcaagc tcgtcgccct
     2281 gcagtacaag cgcaacgatg ccgcctttgc gcgggggaat ttccgcgtgc gcggcgataa
     2341 cctggagatc ttccccagcc actacgagga catggcctgg cgcatctcgt tcttcggcga
     2401 cgaaatcgag gagatcgccg agttcgatcc gctgaccggc aagaagggcg cgagcctcga
     2461 caaggtgcgg gtctatgcca attcgcacta cgttacgccc gggccgacga tgaagcaggc
     2521 gaccgaggcg atccgcttcg aactggccga gcggctgaag gaactggagg cggaaggcaa
     2581 gttgctcgaa caccagcggc tggagcagcg taccaatttc gacctcgaga tgatcgccgc
     2641 caccgggagc tgcgccggga tcgagaacta ttcgcgcttc ctcacgggcc gcctccccgg
     2701 cgaaccgccg ccgaccctgt tcgaatacct ccccgacaac gccctgctgt tcgtggacga
     2761 aagccaccag accgtgccgc agatcggcgc gatgtcgaaa ggcgaccatc gccgcaagat
     2821 caccctggcc gaatacggct tccgcctgcc cagctgcatc gacaaccgcc cgctgcggtt
     2881 caacgaatgg gacgcgatgc gcccgcagac cgtggccgtt tcggcgacgc cgggaccatg
     2941 ggaaatggag caggcgggcg gcgtctttgc cgagcaggtg atccggccca cgggcctgat
     3001 cgacccgccg gtggagatca agccggtgga agaccaggtg caggactgca tcgaggaatg
     3061 ccgcaagacg gcgaagctgg gctaccgcac cctcgtcacc accctcacca agcgcatggc
     3121 cgaggacctg accgagttca tgcacgaggc gggcctgcgg gtgcgctaca tgcactccga
     3181 cgtggagacg ctggaacgca tcgagctgat ccgcgacctg cgcctggggg tctacgacgt
     3241 gctggtgggc atcaacctgc tgcgcgaggg tctggacatt cccgagtgcg ggcttgtctg
     3301 catcctcgat gccgacaagg aagggttcct gcgcagcgag acctcgctga tccagacgat
     3361 cggccgcgcc gcgcgcaacg tggacgggcg ggtgatcctc tatgccgacc ggatgacggg
     3421 aagcatggaa cgcgcgatcg cggaaaccga ccgccgccgg gccaagcagg acgcctacaa
     3481 ccgggagcac gggatcaccc cggccacgat caagcgaaat atccatgaca tcgtggccga
     3541 taccgccagc cgcgacgggg tggtgatcga cctcgaagac gatgcgcgca acaatctcgt
     3601 cggccacaac ctgcgggcct acatcgagga actggaacag cgcatgcggg cggcggcggc
     3661 cgatctcgag ttcgaggaag ccgggcgcct gcgcgacgag atccgccgtc tggaggccga
     3721 ggaactggga ctgcccgagg cggaacacgc cggcggctct cgggcgccgc gcgtgggccg
     3781 gagcaacgag ggcaagccgg ggacgcgcaa gacgcggtat gggaagacgc agcggaagtg
     3841 ggggcgctaa cccccacccg ccgcgttggc ttcaggctcc cgtcagcaga tccgcttcgt
     3901 tgctcttgat cttccagcca ccgccggtgc gcacgagggt catcacgcag cgggccatga
     3961 cgttgggctt gcccggttcg ccgctcatcc acgtggtgac gaagttgtag agatgggcct
     4021 tgatgacgtc cttgctctca ccatagtcgc agaagatgtt cacgcccttg tgggccatgg
     4081 tgtagcccac gctggccttg atcccgatcg ggacctggcg cagcgcttcg cggccgacga
     4141 tcggttcggg gctgatgccg gtgaacacgc cgtcttcgag ccacaggtcg gcccagccat
     4201 cggcatcgcc ggcgtcgacc ttccagcagt agcgcgcgat gtgatcggcg acggcgacat
     4261 agtcatcggt ggaaacgggc atcctggact ctcctcaggt tatctgcgcc gcgaatcgtg
     4321 caattcgtcg gctgatgcgt ggaacttgcc ggcacaggct attgccttcg cggccatgcg
     4381 ccaccagaat ctgcccgatg tcggttgtca ggtcgttcac caccacaagc atgctggttg
     4441 ggggcattct cgccctttcc cttggcggct gttcccaatc ccagagccgc gagctggtcg
     4501 acgccgcgcc cggcggcggc tatcccgcgc gcctggcgct ggccgagctt gcgccggctg
     4561 acgatgccgc gccgctccag cccgacggcg ccggctgggc gcgcaaaggc gacgaggtga
     4621 cgttcggcgt ggccggccag ccggcgctgc tgagccttgc ctgcacccat gacgcagcgg
     4681 gcgcggccag cctcgccctt gcccgccaca cccgcgccga agcgggcgcc aaggcgctgc
     4741 tggcgctgat cgggaatggc cggatcgccc gcctgccgct cgacgtggtg acgccgggcg
     4801 aggccgggga atggcgcggg tcgatcccgg cagcggacgg gcggctcgac gtgctcaggg
     4861 gcggcaaccg gatcgagggc accctgcccg gcggcggcac tctgctgatg ccggcgagcg
     4921 gtgagccggg gcggctgctg gaggaatgcc gggcgagcga ccgcgcggcg gccggggcgg
     4981 tttgagggtt tggtgattgc cccctccaca tccgtggagg gccggtgccg cgcaagggca
     5041 agcgccagct tgcctggtcc atgcattcag agctttccga actggccttc gtagcccgcg
     5101 gtgtcgccgg cggcgaggaa gcgggcctgt tcgacccact tgtcgcgcgc ggggcggccc
     5161 ttgaaggcgg gggggagctg gtcgtcgatc cgggcgaggt ccgcgtccgt ccagccggcg
     5221 acttgcgcgt aggaggttac gcccagctga ccgagcacgg tcgcgagctt ggggccgagg
     5281 cccttgatgc gggtgagatc gtcggcctgc tgctcgggca gtccgggagt cgtttcggcg
     5341 gcgcccgcat cggcggcttc ttcctgcgcg gcggcggcga tcacttcggc gacgccgccc
     5401 agagttcccg cggccggggg gatcgcatag gcggcgctgg cagcgggagc ggcatcgatc
     5461 agggcctggt tacgttccgc ggggccaacg ccttcatcga gcacatcggg gcgatggctg
     5521 cgctgtaccg gccgcgcggc gcggctcatc agccaccagg cgaccagcag ggccaggacc
     5581 acgacgatgg ccaggatcgg ccagttctgt tcaagctgct gcatcgcgca ttccctggag
     5641 gatgaggtta gggctcagat accgcagagc ggcggcggag agaagtcagg gcgcagattg
     5701 gtgaggcgcc agatcaatca ggcaggggcc acttccttgc ggaacaggac gcggtcgtcc
     5761 gggccgaagc ccttgtgcca cagcatccac acgtagatcc cgagaatggc cggaatgccg
     5821 atagcgatca tcgcccattc cgggaggaag gtccgcgccg ccgccccgac gcagactgcc
     5881 ggaaccacgg cccaggcgag cgccccccgg aaattgttga tcggctcgcc caggacacgg
     5941 gccagcaggc gcgctttcag cagcgaggcc gtggccaggg caatcatcag cgcggcggcg
     6001 gcggcggcga tctgccagcc ctccgcatgg ccggaggcgc gcaccagcat gatcccgccg
     6061 accgtgagca ggccctggag gacgatcgtg aacagcgaga ggacgaagtt ctggtagcgc
     6121 gcgacgtag
//
LOCUS       DATNBV010000525         3895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_304466,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000525 DATNBV010000000
VERSION     DATNBV010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304466"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VN627_12440"
     CDS             <1..675
                     /locus_tag="VN627_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HXD04104.1"
                     /translation="CNWKAMLDNFYEGYHVATIHQETVAEWLDTKAMAVDIFRNGHNR
                     MCTTRKIFARIKEGISDGVDGQNVAQASMPIVPDFSDVFRDHTVGMATFPNNQAALEP
                     GGFPWQAMWPVGPGRTVMDLWILGWDDPEIDPAVENAYWDRYLADNERIVSEDMHLLS
                     AIQKSLEGGAFTGTMMGAQERGIYWYHEEIDRRIGVERIPENLRVEQVLAKHVVPDDM
                     AATAPA"
     gene            complement(697..1311)
                     /locus_tag="VN627_12445"
     CDS             complement(697..1311)
                     /locus_tag="VN627_12445"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HXD04105.1"
                     /translation="MNSPVTSWAGPTAERILEAAHKLFSEVGFDATKMESVARAARIS
                     RPTLYGYYRSKEELYQAVLAREGALLQRKMAGIDLAGDPVAVLRSFIGMLFDEFVAGA
                     HFSIIDIKLHKGIRVPRSAVISGAAHREILELVLQRGKDAGLFAQNADSAMFQATTVA
                     LLSGFASSREVLPVLTGVEATSAEAIAHWREHLADVMIASIRHP"
     gene            complement(1417..2289)
                     /locus_tag="VN627_12450"
     CDS             complement(1417..2289)
                     /locus_tag="VN627_12450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HXD04106.1"
                     /translation="MKYGLNVVPVPASQLVEVGQRAEELGFDGVYYGEHIAVPKHLKN
                     PYPGKVGYNWKTIQMECYVALGALAAVTTKMRLGTGITILPIRHPLQTARAITTIDNI
                     SNGRFDLIFGVGSIPEEYEVMGVNYKTRGAMLDEWLDIFDKLWTQEEIEHHGRFMQFE
                     TIGFEPKPVQKPGPPLWSGAWSAAGLERAARRCHGWYGAVYNADGVRQVKELIEPFLK
                     KYDRDPATFHYALIHAAGEDILPTAEEIRDYEAEGVERLILSPFTHPNESFETNAMAK
                     LEACAKGLGSALQG"
     gene            2552..3424
                     /locus_tag="VN627_12455"
     CDS             2552..3424
                     /locus_tag="VN627_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXD04107.1"
                     /translation="MKAISFKPAAGLLLAAIALPSAATAQGQQPQYDAPASGIIVNGQ
                     VPDTAAMTKGPDVKGIITARTGDRIKVATPDGNATVIAVTSATKIKSGSGLFGGKSKL
                     GPEALLNGLPVTVKTLQGGGQLVAAEVSFKGGDLKTANMIRNGTDQRFGEQAAATEAL
                     RGRMGDIDKYNIKGTTNVYFDTGKYNISPQARAELCQTATQAEATENALMLVVGYTDA
                     VGGDEYNQTLSEKRASSVINYLQLCHWKPYRMLTPTGMAKADPAADNSTPEGRAQNRR
                     VSVNILVSKAVDVN"
     gene            complement(3539..>3895)
                     /locus_tag="VN627_12460"
     CDS             complement(3539..>3895)
                     /locus_tag="VN627_12460"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HXD04108.1"
                     /translation="VLVRQSCAISGVTGIALTKVDVLDGLETVKICTGYRLGGKVLDY
                     FPSHAADQAAVEPIYEEMEGWSGTTAGARSWADLPAQAIKYIQRVQELIETPVALVST
                     SPEREDTILVRDPFED"
BASE COUNT          689 a         1217 c         1345 g          644 t
ORIGIN      
        1 tgcaactgga aggcgatgct cgacaacttc tacgagggct accacgtcgc cacgatccac
       61 caggaaacag tggcggaatg gctcgacacc aaggccatgg ccgtcgacat cttccgcaac
      121 gggcacaatc gcatgtgcac cacgcgcaag attttcgcgc ggatcaagga gggcatcagc
      181 gacggggtcg acgggcagaa tgtggcgcag gcgtcgatgc cgatcgtgcc ggacttttcc
      241 gacgtgttcc gcgaccacac cgttggcatg gccactttcc ccaacaacca ggcagcgctg
      301 gagccgggcg gttttccgtg gcaggcgatg tggccggttg ggccgggccg tacggtgatg
      361 gacctgtgga tcctgggctg ggacgatccc gagatcgatc cggccgtcga gaatgcttat
      421 tgggaccgct atcttgccga caacgagcgg attgtgagcg aggacatgca cctgctttcc
      481 gccatccaga aatccctgga gggcggcgcc ttcaccggga cgatgatggg agcccaggaa
      541 cgcggcatct actggtacca cgaggaaatc gaccgccgca tcggcgtgga gcggattccc
      601 gaaaatctgc gggtcgaaca ggtgctggcg aagcatgtgg tgcccgacga catggctgcc
      661 accgcgccgg cctgagcagc ctgtccgggc agcgcgtcag gggtgcctga tcgaggcgat
      721 catgacatcg gccaggtgtt cgcgccaatg ggcgatcgcc tcggccgagg tcgcttcgac
      781 gccggtgaga accggcagca cctcacgcga ggaggcaaac ccgctgagca gggccaccgt
      841 ggtcgcctgg aacatcgcgc tgtcggcgtt ttgcgcgaag agtcccgcat ccttgccgcg
      901 ctgaaggacc aactcgagta tctcgcgatg ggcggcgcct gagatgactg cgctgcgggg
      961 gacgcggatc cccttgtgca gcttgatgtc gatgatcgag aaatgggcgc cggcgacgaa
     1021 ttcatcgaat agcatgccga tgaagctgcg caggaccgca accggatcgc ccgcgaggtc
     1081 gatgccggcc atcttgcgct ggagcagggc tccttcgcgg gcgagaacgg cctggtagag
     1141 ttcttccttg ctgcggtaat agccatacag cgtgggccgg ctgatccggg cggcgcgggc
     1201 gaccgattcc atcttggtcg catcgaaccc cacctcggag aacagtttat gggcggcctc
     1261 gaggatccgc tcggccgtgg ggccggccca tgaggtgacg ggcgagttca tggcgcgagc
     1321 ctatcagcgc cggggcggcg cgcaacgggg gctttggggc gcgtgcccct ccttcccctg
     1381 aggtggtccc cctcccccgg cgggggcggg atgggttcag ccctggaggg cgctgccgag
     1441 acccttggca caggcttcga gcttggccat ggcgttcgtc tcgaagctct cgttggggtg
     1501 tgtgaacggc gacaggatca ggcgttcgac gccttcggct tcgtagtcgc ggatctcttc
     1561 ggcggtgggc aggatatcct cgcccgcggc atggatcagg gcatagtgga aggtggccgg
     1621 atcgcggtcg tacttcttga ggaagggttc gatcagctcc ttcacctggc gcacgccatc
     1681 ggcattgtag accgcgccgt accagccatg gcagcggcgc gcggcgcgtt cgaggccggc
     1741 agcagaccag gcgccggacc acagcggcgg cccgggcttc tgcacgggct tcggttcgaa
     1801 gccgatcgtc tcgaactgca tgaagcggcc gtggtgttcg atctcttcct gggtccagag
     1861 cttgtcgaag atgtcgagcc actcatcgag catggcgccg cgcgtcttgt agttgacgcc
     1921 catgacctcg tactcctcgg ggatcgagcc gacgccgaag ataaggtcga accggccgtt
     1981 cgagatattg tcgatcgtgg tgatggcccg cgcggtctgc agcgggtggc ggatcgggag
     2041 gatggtgatg ccggtgccga ggcgcatctt ggtggtgacg gcggcgagtg cgcccagcgc
     2101 gacgtagcac tccatctgga tggtcttcca gttgtagccg accttgccgg gatagggatt
     2161 tttcaggtgc ttgggcacgg cgatgtgttc gccatagtac accccgtcaa acccgagttc
     2221 ctcggcccgc tggcccactt cgacgagctg ggaggcgggg accggaacga cgttgagccc
     2281 gtatttcatg gatgcatcct ctctcggccg cggggcaggc cgggcctgct tcctcgctgg
     2341 gcggagggaa acactccgtg cggaaaaggg caacaggggg attgcgccaa tcgtggatgt
     2401 gctgccaggg gccggggcga cgcggccact cggttcgtcg cagggcgggc ccgggccagc
     2461 catccggcga gcctagcttg cggcacgttc attgggccgc aaggttatgg ccaacaggcg
     2521 caagtggact ggggatacga aggagttgtt catgaaagcc atttcattca agcccgcagc
     2581 cgggctcctg ctggccgcga ttgccctgcc gtcggcagcc accgcccagg ggcagcagcc
     2641 gcaatatgac gcgcccgctt cgggcatcat cgtcaatggc caggtgccgg acaccgccgc
     2701 catgaccaag ggcccggacg tcaagggcat catcaccgcg cgcaccggcg accggatcaa
     2761 ggtggccacg cccgacggca acgccacggt gatcgcggtg accagcgcga cgaagatcaa
     2821 gagcggctcc ggcctgttcg gcgggaagag caagctgggg cccgaggccc tgctcaacgg
     2881 gctgccggtg accgtcaaga ccctgcaggg cggcgggcag ctggtggcgg ccgaagtcag
     2941 cttcaagggc ggcgacctca agaccgccaa catgatccgc aacggcaccg accagcgctt
     3001 cggcgagcag gccgccgcga ccgaggccct gcgcgggcgc atgggcgaca tcgacaagta
     3061 caacatcaag ggcacgacca acgtctactt cgacaccggc aagtacaaca tctcgcccca
     3121 ggccagggcc gagctgtgcc agacggcaac ccaggccgag gcgacggaaa atgcgctgat
     3181 gctggtggtt ggctataccg atgcggtggg cggcgacgaa tacaaccaga ccctgagcga
     3241 gaagcgcgcc agcagcgtga tcaactatct ccagctctgc cactggaagc cctatcgcat
     3301 gctgaccccc accggcatgg ccaaggccga tccggcagcg gacaattcga cacccgaagg
     3361 ccgcgcgcag aaccggcgcg tttcggtgaa tatcctcgtc agcaaggcgg ttgacgtgaa
     3421 ctgagcgagg cgggggcggc catgggctgc cccctccctg ttccattcaa gaggagggtg
     3481 agcgcttgcc cgctagccca ccccaacccc tcccgcctgc gggaggggct tttttggctc
     3541 agtcctcaaa gggatcgcgc acgaggatcg tgtcttcgcg ttcggggctg gtggagacca
     3601 gggcgaccgg ggtttcgatc agctcctgca cgcgctggat gtacttgatc gcctgcgcgg
     3661 gcagatcggc ccacgaacgg gcgccggcgg tggtgccgga ccagccttcc atttcctcgt
     3721 aaatcggctc gaccgcggcc tgatcggcgg cgtggctggg gaagtagtcc agcaccttgc
     3781 cgcccaggcg atagccggtg cagatcttca ccgtctcgag gccatcgagc acgtcgacct
     3841 tggtcagcgc aatgccggtc acgccggaga tggcgcagga ctggcgcacc agcac
//
LOCUS       DATNBV010000526        11346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3793977,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000526 DATNBV010000000
VERSION     DATNBV010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11346
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3793977"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..2763
                     /gene="metH"
                     /locus_tag="VN627_12465"
     CDS             151..2763
                     /gene="metH"
                     /locus_tag="VN627_12465"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HXD04109.1"
                     /translation="MTQSTARFVNIGERTNVTGSAAFKKLILAGDYAKAVEVARQQVE
                     NGAQVIDVNMDEGLLDAVAAMTTYLKLIAAEPDIARVPVMIDSSKWEVIEAGLKCVSG
                     KPIVNSISMKEGEEPFLAAARKCMDYGAAVVVMAFDEKGQADTKARKVEICTRAYNLL
                     TGIGFPPEDIIFDPNVFAVATGIEEHNNYAVDFIEAVKEIRVACPHVHFSGGLSNLSF
                     SFRGNEPVRRAMHSVFLYHAIPAGLDMAIVNAGQLDVYDQIDPALRVACEDVILNRDP
                     DATERLIALAESFKGKDAVAEKQAEEWRGWDPVKRIEHALVKGLDAFIVEDTEEMRAA
                     VAARGGRPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAHLIPFIEAE
                     KDENSRAKGRIVMATVKGDVHDIGKNIVGVVLQCNGYEVIDLGVMVPWSKILETAAQE
                     KADIIGLSGLITPSLDEMVTVAEEMQRAEMTIPLLIGGATTSKVHTALRIDPAYDGPV
                     IHVLDASRAVGVASQLLSDTQATGFIKTTAADYQHVRDARAGKGQSELLPLADARDNA
                     YPADMSEKAPPPEQPGLHVFEDWDLADLVETFDWTPFFRAWELAGVYPAILDDDVVGE
                     SARSLYADARAMLDKIVSEKWLTAKGVVGLWPCARHGDDVVLHPDSDETSVRLPFLRQ
                     QFAKSRGRANFCLADFIDPAGDWLGGFAVGIHGIEPHLARFKAAHDDYSDILLKALAD
                     RFAEAFAERLHQHVRTTLWAYAPGELLTNEALIREQYRGIRPAPGYPACPDHSLKPIL
                     FDLLKAQETAGITLTESQAMLPTSAVSGFYFAHPESQYFGVARIGRDQLEDYAARRGV
                     DLATAERWLRPNLD"
     gene            2864..3325
                     /locus_tag="VN627_12470"
     CDS             2864..3325
                     /locus_tag="VN627_12470"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HXD04110.1"
                     /translation="MDRRVFRPRIAVGGVPIHAMLVPFPIVCFTGALLTDLAYAASAQ
                     VQWTNFSQWLLLFGTVFAGLAAVFGLIDYFSAPRERRPAIGLWHMALNLVLFVVAVFN
                     NLVHARDGWTSVVPTGLTLSIITMLLLIVSGHLGHRLAYRHVVRNLEEDRP"
     gene            3322..4665
                     /locus_tag="VN627_12475"
     CDS             3322..4665
                     /locus_tag="VN627_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HXD04111.1"
                     /translation="MKRLTALLLTGAAPLIVLAACSRSAEPPTQFGFNPKLPEPRQFL
                     LPPMGIANPVGWDKTGAGAVPTVPPGFVVQAFAKGLNSPRNVLPLPNGDIIVATAGGP
                     DMEPNLRPKSFIFQWLMNTSHGGEKGANSGENALLLLRDTNHDGLADQTITLAKDLPS
                     PFGLAWGNGTLYVGMTDSVWRYPLAAGQAALGPGSLVTELPGGPIDHHWTKSITLSPD
                     GTKLYAGVGSNSNIVENGLEAEHGRAMIWEVDPATGARRPFATGLRNPNGLTFYPGSN
                     TLYAVVNERDELGNNLVPDYMTSVRPGAFYGWPWSWYGQHVDVRVQPQRPDMVAKAVI
                     PDYSLSGHVAALGLAFLKGGDFPAHYQGGAFIGEHGSWDRNVLNGYQVAFVPFVNGRP
                     SGTPETFLGGFTTGKGHDVHGRPVGLAFGPDGALYVADDAGKTIWRISWRGNGGT"
     gene            4725..6515
                     /gene="recQ"
                     /locus_tag="VN627_12480"
     CDS             4725..6515
                     /gene="recQ"
                     /locus_tag="VN627_12480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HXD04112.1"
                     /translation="METAAPDLHDALHATFGFTAFRGVQEDVVRRVLAGRSTLAVMPT
                     GAGKSLTYQLPAVMLDGTCVVVSPLIALMHDQLRSARANGIRAATLTSADMDREATID
                     GFRAGELDLLYVAPERASQPHFRELLSRARVSLFAIDEAHCVSEWGHDFRPDYRLLRP
                     LLDEFPQVPRLALTATADAHTRADILTQLGIPDDGLIVAGFDRPNIRYTITGRDQPVQ
                     QLKRLMAENPGPGIVYAQTRAQVERLAEQLGATGRSVLPYHAGLAPEVRAGNQAAFVA
                     SEDMVMVATIAFGMGIDKPDVRFVAHAGLPKSIEAYYQETGRAGRDGDPSVAVMLWGA
                     DDFVRARQRLGEVDQSRLPSERARLDALAALVETATCRRAILLRHFGEDPPATCGNCD
                     NCLDAPGVTDGTMTAQKLLSAVYRTGQTYGLGHVEKVLTGASDPRVIERGHDQLSVFG
                     IVGEDEARLIRPIARALQARGALLATEHGGLRLGGDAKAILKGEAEVNVVVPPKKERT
                     GRRARGGAGAGGAANPVGDPLFDALRGLRRELALEAGVPPYVVFHDSVLREMAATRPS
                     SRAELSAIGGVGTRKLEAYGDAFLSLIRQF"
     gene            complement(6502..6678)
                     /locus_tag="VN627_12485"
     CDS             complement(6502..6678)
                     /locus_tag="VN627_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXD04113.1"
                     /translation="MSDRFFRLLERHQRLDAELRDAQRRRWVDPLEIVRLKKLKLALK
                     DRLSKLARRRPRTA"
     gene            7008..7907
                     /locus_tag="VN627_12490"
     CDS             7008..7907
                     /locus_tag="VN627_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXD04114.1"
                     /translation="MNDEPVAMTSSRERAAWSTSGYLMLVIFLALAFLLVVRIGTFNG
                     EDGQVGTFLGGVFLLPIALIVIAKGFYMIQPNQAAAITLFGSYRGTDRNHGLRWVWPW
                     MGKTRVSVRANNVVSEKLKVNDLRGNPIEIATNVVWRVADTAQALFDVDDYKAFVFVQ
                     IEAAVRAIGSRYPYDDIEHMEVTLRGSHDQINAELRTELNSRLAIAGITVDECGLTHL
                     AYAPEIAGAMLRRQQAEAVIAARRKLVEGAVSMVEMALTQLSEKNVVHLDDDRRAAMV
                     ANLMVVLCGERDTQPVVNTGMGA"
     gene            8021..8956
                     /locus_tag="VN627_12495"
     CDS             8021..8956
                     /locus_tag="VN627_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXD04115.1"
                     /translation="MRKIPLTAALALLAAAPSLAQSEAPYTVVETGQRFGRLQDAVNA
                     IGDGMGTIRLAGGDHRDCAVQERGDVAYVAATPGAATLSGTACEGKASLVLRGRSARV
                     EGLVFTGIRVADFNGAGIRIERGNLAVSQAWFHDSQQGILSAGDPAGTITVDKSTFTR
                     LGTCEGGGGCAHSLYIGEYGALTVTRSRFEAGRGGHYLKSRAARVTILDNSFDDAAGK
                     GTNYMIDLPNGATGRIAGNWLIQGANKENHSAFIAIAAEGRGNSSAGLAVEGNVARLG
                     PGVSWRSVFVADWSGDPLAIGANQLGDGLTRFERR"
     gene            8995..9339
                     /locus_tag="VN627_12500"
     CDS             8995..9339
                     /locus_tag="VN627_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04116.1"
                     /translation="MIAALPRLLLARLLGALMLLTVALQAGMPFAAPLERVHGSAFSA
                     ATHEVALTTDRRVQAVRPALAPEPRLPAIEPLSAPPPPLAAPITPRPFSTGPPPREEI
                     ARRPAPRAPPFA"
     gene            9391..9684
                     /locus_tag="VN627_12505"
     CDS             9391..9684
                     /locus_tag="VN627_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04117.1"
                     /translation="MSQSEAARAARRGEIEAMLARYPHLSPEAVADLGQWFEKEASSL
                     DIGLIACNDAIAEPYRAFRADHVDPLKGKDWLRGIAFAAVILAIMGVLFWRAF"
     gene            complement(9721..10161)
                     /locus_tag="VN627_12510"
     CDS             complement(9721..10161)
                     /locus_tag="VN627_12510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HXD04118.1"
                     /translation="MTAAAFATAQHSDGNEAEVIDRLRADGDLALSLVAVAGGIVGHV
                     AFSPVQISDGSRAWYGLGPVSVTPEFQRRGAGAALVRAGLALLRERSARGCVVLGNPD
                     YYARFGFVHDPVLAYPGPPARFFQRLVLHGAPPSGVVTYAPGFG"
     gene            complement(10218..11282)
                     /gene="ribA"
                     /locus_tag="VN627_12515"
     CDS             complement(10218..11282)
                     /gene="ribA"
                     /locus_tag="VN627_12515"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HXD04119.1"
                     /translation="MRAVTSAPRRLALALDALRHGWPVVVDGVLSLLPAETGFGPGVS
                     APWMLISAARAVTLKLANQREAAAPDAPVLIRGAEAFDLVHARALADPALDLRFPLKG
                     PFRAEHIDHAEAARTAMELARLAGILPAFLVAPEPLGEAQPVSSADLAAWKEPAALVI
                     ASRARLPVAACEDTEIVAFRSADDLREHVALVIGEQNGDRAPLVRLHSECLTGDILGS
                     LKCDCGPQLHAALEAMAEEARGGGWGVLLYLRQEGRGIGLVNKLRAYRLQDLGFDTVE
                     ANNRLGLPTEARDFPVAARMLELLGVHAIRLMTNNPAKVDALAACGVTVAQRVAHKLP
                     ANPHNERYLATKRDRTGHLL"
BASE COUNT         1854 a         3958 c         3774 g         1760 t
ORIGIN      
        1 taaaaatcct ccccgctgac ggggaggggg accacccgaa gggtggtgga gggggctcac
       61 cggctacgcc gcgcccgccc ccaagccccc tccaccgtcc tgcggacggt ccccctcccc
      121 accccgtggg gaggatcgaa agagaagacg atgacccaat ccactgcccg cttcgtcaac
      181 atcggcgagc gcaccaacgt taccggctcg gccgcgttca agaagctgat ccttgccggc
      241 gattatgcca aggcggtgga agtcgcgcgc cagcaggtcg agaacggcgc ccaggtaatc
      301 gacgtcaaca tggacgaggg cctgctcgac gcggtcgcgg ccatgaccac ttacctcaag
      361 ctgatcgccg ccgagcccga catcgcacgg gtgccggtga tgatcgacag ctccaagtgg
      421 gaagtgatcg aggccgggct caagtgcgtc tcgggcaagc cgatcgtcaa ctcgatcagc
      481 atgaaggagg gcgaggagcc gttcctcgcc gctgcgcgca agtgcatgga ctatggcgcg
      541 gccgtcgtcg tcatggcctt cgacgagaaa ggccaggccg acaccaaggc gcgcaaggtc
      601 gagatctgca cgcgggccta taacctgctg accggcatcg gcttcccgcc ggaagacatc
      661 atcttcgatc ccaacgtttt cgcggtcgcc accgggatcg aggagcacaa caactacgcg
      721 gtcgacttca tcgaagcggt gaaggaaatc cgcgttgcct gcccgcacgt ccacttctcg
      781 ggcggcctgt ccaacctctc cttctcgttc cgcggcaacg agccggtgcg ccgcgcaatg
      841 cattcggtgt tcctctacca tgcgatcccc gccgggctcg acatggcgat cgtcaacgcc
      901 ggccagctcg acgtctacga ccagatcgac ccggccctgc gcgtggcctg cgaggacgtg
      961 atcctcaatc gcgatcccga tgcgaccgag cggctgattg cccttgccga aagcttcaag
     1021 ggcaaggatg ccgtcgccga gaagcaggcg gaggaatggc gggggtggga ccccgtcaag
     1081 cggatcgagc atgcgctggt gaagggcctc gatgccttca tcgtcgagga taccgaggag
     1141 atgcgggcag ccgtggcggc gcgcgggggg cggccgatcg aggtgatcga aggcccgctg
     1201 atggacggca tgaacgtcgt cggcgacctg ttcggctccg gcaagatgtt cctgccgcag
     1261 gtggtgaaga gcgcccgcgt gatgaagaag gcggtcgccc acctgatccc cttcatcgag
     1321 gcggagaagg acgagaactc cagggccaag ggccggatcg tcatggccac ggtcaagggc
     1381 gacgtgcacg acatcggcaa gaacatcgtg ggcgtggtgc tccagtgcaa cggctacgag
     1441 gtgatcgacc tcggcgtcat ggtgccgtgg tcaaagatcc tcgaaaccgc cgcgcaagaa
     1501 aaggcggaca tcatcggcct gtcgggcctg atcacgccct cgctggacga gatggtgacc
     1561 gtggccgagg aaatgcagcg cgccgagatg accatcccgc tgctgatcgg cggggcgacg
     1621 acctccaagg tccacaccgc gctgcgcatc gatccggcct atgacggccc cgtgatccac
     1681 gtgctggatg ccagccgcgc ggttggcgtt gccagccagc tgctgagcga cacccaggcg
     1741 accggcttca tcaagaccac cgccgccgat taccagcacg tgcgcgacgc gcgcgccggc
     1801 aagggccaga gcgagctgct gccgctggcc gacgcgcgcg acaatgccta tcccgccgac
     1861 atgagcgaaa aggccccgcc gccggagcag ccgggcctgc acgtgttcga ggactgggat
     1921 ctcgccgacc tggtcgagac cttcgactgg acgccgttct tccgggcctg ggagctggcc
     1981 ggggtctatc ccgcgatcct cgacgacgat gtcgtcggcg agagcgcccg cagcctctat
     2041 gccgatgcca gggccatgct ggacaagatc gtcagcgaga agtggctcac cgcgaagggc
     2101 gtggtcggcc tctggccctg cgcccgccat ggcgacgacg tggtgctcca ccccgacagc
     2161 gacgagacca gcgtgcgcct gccgttcctg cgccagcagt tcgccaagag ccggggccgc
     2221 gccaacttct gccttgccga tttcatcgac ccggcgggcg actggcttgg cggcttcgcg
     2281 gtcggcatcc acgggatcga gccgcacctc gcccgcttca aggccgcgca cgacgattac
     2341 tccgacatcc tgctcaaggc cctggcggac cgcttcgccg aggccttcgc cgagcgcctg
     2401 caccagcacg tgcgcaccac cctgtgggct tatgccccgg gcgagctgct caccaacgaa
     2461 gccctgatcc gcgagcaata ccgcggcatc cgcccggcgc cgggctaccc cgcctgcccg
     2521 gaccactcgc tcaagccgat cctgttcgat ctgctgaaag cgcaggagac cgccgggatt
     2581 accctgaccg aaagccaggc catgctgccg accagcgcag tctccggctt ctacttcgcc
     2641 caccccgaaa gccagtactt cggcgtcgcg cggatcgggc gcgaccagct ggaggactat
     2701 gcggcacggc gcggggtgga cctggccacg gcagagcgct ggctacggcc gaacctggac
     2761 tgaccgtctc gatcctcccc tgcaaccaag tccgcccacc gtcgttaccc ccaaggctgc
     2821 gtgcagccgg ggctgacgac gacaacgaca ggcggagacg tcaatggacc ggcgtgtttt
     2881 ccggccgagg atcgccgtgg gcggcgtgcc cattcatgcg atgctcgtgc ctttcccgat
     2941 cgtctgcttt accggcgcgc tgctgaccga ccttgcctat gcggcgagcg cgcaggtgca
     3001 gtggaccaac ttctcgcagt ggctgctgct gttcggcacg gtcttcgccg ggctggcggc
     3061 cgtgttcggc ttgatcgact acttcagcgc accgcgtgag cggcgtccgg caatcggcct
     3121 gtggcacatg gcgctgaacc tcgtcctgtt cgtcgtcgcc gtgttcaaca acctcgttca
     3181 cgcccgcgac ggttggacca gcgtggtgcc gacgggcctc accttgtcga tcatcaccat
     3241 gctgctgctg atcgtcagcg gccatctggg gcaccggctg gcctaccgcc acgtggtgcg
     3301 caatcttgag gaggaccggc catgaagcgc ctcactgccc tcctgctgac aggagccgcc
     3361 cccctcatcg ttctggccgc ctgcagccgc agcgccgagc cgccgaccca gttcgggttc
     3421 aaccccaagc tgcctgaacc caggcaattc ctgctgccgc cgatggggat cgccaatccg
     3481 gtcggctggg acaagaccgg cgccggggcc gtgcccaccg tgccgccggg cttcgtggtg
     3541 caggcctttg ccaaggggct caattcaccg cgcaacgtgc tgccactgcc caatggcgac
     3601 ataatcgtcg ccaccgcggg cggacccgac atggagccga acctgcggcc caagtcgttc
     3661 atcttccagt ggctgatgaa cacctcgcac ggcggcgaaa agggcgcgaa ctccggtgag
     3721 aacgccctgc tgctgctgcg cgacaccaac cacgatggcc tcgccgacca gaccataacc
     3781 ctcgccaagg acctgccctc gccgttcggc ctcgcctggg ggaacggcac gctctacgtc
     3841 ggcatgacgg attcggtctg gcgctatccc ctggcggcgg ggcaagccgc gctgggcccg
     3901 ggctcgctcg taaccgaact ccccggcggc ccgatcgacc accactggac caagtcgatc
     3961 accctgagcc ccgatggcac caagctctat gcgggcgtgg gctccaattc caacatcgtc
     4021 gagaacgggc tcgaggccga gcacgggcgg gcaatgatct gggaagtcga tccggcaacc
     4081 ggagcgcggc ggccgttcgc cacgggcctg cgcaatccca atggactgac cttctacccc
     4141 ggctcgaaca cgctctacgc cgtggtcaac gagcgtgacg aactgggcaa caatctcgtc
     4201 cccgactaca tgacgtcggt caggccaggc gcgttctatg gctggccgtg gtcgtggtat
     4261 ggccagcatg tcgacgtgcg ggtccagccg cagcggccgg atatggtggc gaaggccgtg
     4321 atccccgact actcgctttc gggacacgtc gccgccctcg gcctcgcctt cctgaaaggg
     4381 ggcgacttcc ctgcgcacta ccagggcggt gccttcatcg gcgaacatgg gtcgtgggac
     4441 cgcaacgtgc tcaacggcta ccaggtggcc ttcgtgccct tcgtcaacgg ccggccgagc
     4501 ggcacgcccg agactttcct cggtgggttc accaccggca aggggcacga tgtgcatggc
     4561 cgcccggtgg ggctggcctt cggtcccgat ggcgcgcttt atgtcgcgga cgatgccggg
     4621 aagacgatct ggcgcatatc gtggcgtgga aacggcggaa cctgagcatt cttcgctgag
     4681 ctgcggttcc gcagccccgc gcgatctgcc atagcggtgc ggtgatcgaa acggccgcgc
     4741 ccgacctgca tgacgcgctc cacgcaacct ttgggttcac cgcatttcgc ggggtccagg
     4801 aggatgtggt gcgccgcgtg ctggccgggc gctccaccct cgccgtcatg cccaccggag
     4861 cgggcaagtc gctgacctac cagctaccgg cggtgatgct ggatggcacc tgcgtggtgg
     4921 tctccccgct gattgcgctg atgcatgacc agctgcgctc ggccagggcc aatggcatcc
     4981 gcgccgcgac gctgaccagc gccgacatgg accgcgaggc gacgatagac ggcttccgcg
     5041 ccggtgagct ggacctgctc tacgtcgccc cggaacgggc cagccagccg catttccgcg
     5101 agctgctgag ccgggcccgg gtatcgcttt tcgcgatcga cgaggcgcac tgcgtttccg
     5161 aatggggcca cgattttcgg ccggattacc gcctgctgcg gcccttgctg gatgaatttc
     5221 cgcaggtccc ccgcctcgcg ctcacggcca cggccgatgc ccacacccgg gccgacattc
     5281 tcacccagct cggcattccc gatgacggcc tgatcgtcgc cgggttcgac cggccgaata
     5341 tccgctacac cattaccggg cgcgaccagc cggtgcagca gctgaagcgg ctgatggcgg
     5401 agaacccggg gccgggcatc gtctatgcgc agacccgcgc gcaggtggag cggctggccg
     5461 agcagctggg cgctaccggc cgatcggtgc tgccctatca cgcgggcctc gcgccggaag
     5521 tgcgcgccgg caaccaggcc gcctttgtcg ccagcgagga catggtgatg gtggcgacca
     5581 tcgccttcgg catggggatc gacaagcccg acgtgcgctt cgtcgcccat gccggcctgc
     5641 cgaaatccat cgaggcctac tatcaggaaa ccggccgtgc cggacgcgat ggcgatccgt
     5701 cggtggcggt catgctgtgg ggtgccgacg atttcgtgcg ggcccggcag cggctcgggg
     5761 aagtcgacca gtcccgcctg cccagcgagc gggcacgcct cgatgccctc gccgcgctgg
     5821 tcgagaccgc cacctgccgc cgcgcgatcc tgctgcggca cttcggcgag gacccgccgg
     5881 ccacctgcgg caactgtgac aactgcctcg atgcgcctgg cgtgaccgac ggaacgatga
     5941 ccgcccagaa actgctgtcc gccgtttacc gcaccgggca gacctatggc ctgggccatg
     6001 tcgagaaggt gctgacgggg gcgagcgatc cgcgcgtgat cgagcggggg catgaccagc
     6061 tttcggtctt cggcatcgtg ggcgaggacg aggcgcggct gatccggccg atcgcccgcg
     6121 ccctccaggc ccgcggcgcg ctgcttgcca ccgagcatgg cgggctgcgg ctcggcggcg
     6181 atgccaaggc gatcctcaag ggcgaggccg aggtgaacgt ggtcgtgccg ccgaagaagg
     6241 agcgcaccgg gcgccgggcg cggggcggcg caggcgcggg cggcgcggca aacccggtcg
     6301 gcgatccgct gttcgacgcc ctgcgcggcc tgcgccgcga actggcgctg gaggcgggcg
     6361 tgccgcctta tgtggtgttc cacgattcgg tcctgcgcga gatggcggcc acccggccat
     6421 cttcccgcgc agaactttcc gccatcggtg gggtcggcac ccgcaagctc gaggcttacg
     6481 gggacgcctt cctctcgctg atcaggcagt tctaggcctg cgccgcgcca gcttcgaaag
     6541 gcggtccttc agcgccagct tgagcttctt gagccgcacg atctccagcg gatcgaccca
     6601 gcgacggcgc tgggcatcgc gcagttcggc atcgagccgc tggtggcgct ccagtaagcg
     6661 gaagaaacgg tcggacatga taatgctcct cgaaacacag gttgcttcga tcggatgtgc
     6721 cgacgccggg cttaggggga acgggggagg acccgggcca cagcatcggc aacatccgat
     6781 gcggtccgac atcacgcagc gcacctgccg aatggccagg tccggaaatt ggcgccgggg
     6841 ttacaatgcg cctgtccggt agagcgccgc gccagagggg cccggtccgc ccgatctcat
     6901 taagagtgat tcggtctttt atcaagcccc gacgccggca cctccgttag cttgcccgaa
     6961 aggacagcgg tggtaggttc tgccgatcct ttgcaagggg gcgggcaatg aacgacgaac
     7021 ctgtggcgat gacctccagc cgcgagcgcg cggcatggag caccagtggc tatctcatgc
     7081 tggtgatctt ccttgcgctc gccttcctgc tggtggtgcg gatcggtacc ttcaatggcg
     7141 aggacggcca ggtcggcaca ttccttgggg gcgtgttcct gctgccgatt gccctgatcg
     7201 tgatcgccaa gggcttctac atgatccagc ccaaccaggc cgcggcgatc accctgttcg
     7261 gctcgtaccg cgggaccgac cgcaaccacg ggctgcgctg ggtctggccg tggatgggca
     7321 agacgcgggt ttcggtccgc gccaacaacg tcgtttccga aaagctcaag gtcaacgacc
     7381 tgcgcggcaa tccgatcgag atcgccacca acgttgtctg gcgcgtggcc gataccgccc
     7441 aggccctgtt cgacgtcgac gactacaagg ccttcgtgtt cgtccagatc gaggctgcgg
     7501 tccgcgcgat cggctcgcgc tacccctatg acgacatcga acacatggaa gtgaccctgc
     7561 gcggcagcca cgaccagatc aacgccgagc tgcgcaccga actcaacagc cgcctcgcca
     7621 tcgccgggat cacggttgac gaatgcgggc tgacccacct cgcctatgcc cccgagatcg
     7681 ccggcgccat gctgcgccgc cagcaggccg aagcggtgat cgccgcccgg cgcaagctgg
     7741 tggaaggcgc ggtatcgatg gtggagatgg cgctgaccca gctttcggag aagaacgtcg
     7801 tccatctcga cgacgaccgc cgcgcggcga tggtggccaa cctgatggtc gtgctgtgcg
     7861 gggagcgcga tacccagccg gtggtgaata cggggatggg cgcttagacc ctggttgggg
     7921 gtgaggacgc cgaataagcc acgcccaaca atccgcccca ccccgggaca ccccgttcgc
     7981 ccctctccgc accgggcaag tttccggata gtctccctcc atgcgcaaga tccccctcac
     8041 cgccgccctc gccctgctcg ccgccgcgcc ttcgctggcc caatccgaag cgccctacac
     8101 cgtcgtcgag accggccagc ggttcggccg cctgcaggat gcggtgaacg ccatcggcga
     8161 tggcatgggc actatccgcc tcgccggtgg cgaccaccgc gactgcgcgg tgcaggagcg
     8221 gggcgatgtg gcctatgtcg ccgccacgcc gggcgcggcc acgctttcgg gcacggcctg
     8281 cgagggcaag gcgtcgctgg tcctgcgcgg gcgatcagcc cgggtcgagg gactggtttt
     8341 caccggcatc cgcgtggccg acttcaacgg cgcgggcatt cgcatcgagc ggggcaatct
     8401 cgccgtcagc caagcatggt tccatgacag ccagcagggc atcctctcgg ccggcgatcc
     8461 ggccggcacg atcacggtcg acaagtccac cttcacccgg ctcggcacgt gcgagggcgg
     8521 cggcggctgt gcccattcgc tctacatcgg cgaatatggc gcactgacgg tcacccgcag
     8581 ccggttcgag gccggacgcg gcgggcatta cctgaaaagc cgcgcggccc gcgtcaccat
     8641 cctcgacaat tcgttcgacg atgccgccgg caagggcacc aactacatga tcgacttgcc
     8701 caacggggcg acggggcgga ttgccggcaa ctggctgatc cagggtgcaa acaaggaaaa
     8761 ccattccgcc ttcatcgcca ttgccgccga ggggcgcggc aacagctcgg cgggcctggc
     8821 cgtagaaggc aacgtcgccc ggctcgggcc cggcgtttcc tggcgcagcg tgttcgtggc
     8881 ggattggtcg ggcgatccgc tggcgatcgg cgccaaccag ctgggcgacg ggctgacaag
     8941 gttcgaacgg cgctgaaatt tgccttccag ccaatgactt cctagatggt gacgatgatc
     9001 gccgcgctcc cccgtctgct gctcgcgcgc ctgctgggcg ccctgatgct gctgaccgtg
     9061 gcgcttcagg ccggcatgcc ctttgccgcc ccgcttgagc gggtgcacgg ctcggcgttc
     9121 agcgccgcga cccatgaagt ggcgctgacg accgaccggc gcgtgcaagc ggtccgccca
     9181 gcgctcgcgc ccgaaccgcg cctgcctgcg attgagccgc tatcggctcc gcctccgcct
     9241 ctcgccgcac cgatcacgcc gcgtccattc tcgaccggtc cgccgccgcg cgaagagatc
     9301 gcccggcgcc ctgctccacg cgccccgcct ttcgcctgaa cagacccgtt cggcggcatt
     9361 gccgccttca actgttcagg agtcctaccc atgtcccaat ccgaagccgc ccgcgccgcc
     9421 cggcgcggcg agatcgaagc catgcttgcg cgctatccgc atctctcacc cgaagccgtc
     9481 gccgacctcg gccagtggtt cgagaaggag gcgagttcgc tcgacatcgg cctcatcgcc
     9541 tgcaacgacg ccatcgccga gccttaccgc gcctttcgcg cggatcacgt cgacccgctg
     9601 aaagggaagg attggctgcg cggaatcgcg ttcgcggcgg tgatcctcgc gatcatgggc
     9661 gtgctgttct ggcgcgcgtt ctgagcacgg ccgggggtgg gcatcgcctg cccccgccgg
     9721 tcagccgaag cccggggcat aggttaccac ccccgagggc ggcgcgccgt gcagcacaag
     9781 ccgctgaaag aaccgtgcgg gcgggcccgg ataggctagc acagggtcgt gaacgaaccc
     9841 gaagcgggcg taatagtccg ggttgccgag taccacgcag ccgcgggccg accgttcgcg
     9901 cagcagcgcg aggccggcgc ggaccagcgc ggccccggct ccgcgccgct ggaactcggg
     9961 cgtgacggac accggtccaa gaccatacca ggcgcggctg ccatcactga tctgcaccgg
    10021 cgagaaggct acatggccga cgataccgcc tgcaactgcc accagggaca aggcgagatc
    10081 gccatcggcc cgaagccggt cgatgacctc ggcctcgttg ccgtcactat gctgcgcggt
    10141 cgcgaaagca gccgcagtca cctgcgcgat cgccgcaata tccgagggcg cctccgcccg
    10201 gactgtccac ccgctcacta cagcaggtgt ccggtccggt cgcgcttggt ggcgaggtag
    10261 cgttcgttgt gcggattggc gggaagcttg tgggccaccc gctgcgccac ggttaccccg
    10321 caggcggcga gcgcgtccac cttggccgga ttgttggtca tcagccggat cgcatggacg
    10381 ccaagcagct ccagcatccg ggccgccacg gggaaatcgc gcgcctcggt cggcaggccg
    10441 agccggttgt tggcctcgac cgtgtcgaac cccaggtcct gcaggcgata ggcgcgcagc
    10501 ttgttgacga gcccgatccc gcgtccttcc tggcgcagat agagcaggac gccccacccg
    10561 ccgccgcgcg cttcctcggc catggcctcc agcgcggcgt gcagctgcgg cccgcaatcg
    10621 cacttgaggc tgccgaggat atcgccggtc aggcattcgg agtggagccg cacgagcggc
    10681 gcgcggtcgc cgttctgctc gccgatgacc agcgcgacat gctcgcgcag gtcatccgcg
    10741 ctgcggaagg cgacgatctc ggtgtcctcg caggcggcca ccggcaggcg ggcgcggctg
    10801 gcgatgacca gcgccgccgg ttctttccag gccgcgagat cggcggacga caccggctgc
    10861 gcctcgccca gtggttcggg agcgacgaga aaggctggga gaatgcccgc cagccgcgcc
    10921 agctccatcg ccgtgcgggc agcctcggca tgatcgatat gctcggcgcg gaacgggccc
    10981 ttcaggggga agcgcagatc cagcgccgga tcggcaaggg cgcgggcgtg aaccagatcg
    11041 aaggcttccg cgccccggat cagcaccggc gcatcgggcg cggctgcctc gcgctggttg
    11101 gcaagcttga gcgtcaccgc gcgcgccgcc gagatcagca tccagggcgc gctcaccccg
    11161 gggccaaagc ccgtctccgc cggaagcagg gagagcacac cgtcgaccac caccggccag
    11221 ccatggcgca gcgcatcgag cgcgagcgca aggcggcgcg gggccgatgt cacagcgcga
    11281 attccgtgac gaggggcacg tggtccgagg gctggtccca gcgccgggtt tcctcaacca
    11341 agcggt
//
LOCUS       DATNBV010000527         3484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4766677,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000527 DATNBV010000000
VERSION     DATNBV010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4766677"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(421..1653)
                     /gene="metK"
                     /locus_tag="VN627_12520"
     CDS             complement(421..1653)
                     /gene="metK"
                     /locus_tag="VN627_12520"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HXD04120.1"
                     /translation="MRSEYLFTSESVSEGHPDKVADQISDAIVDLFLGKDPEARIACE
                     TLTTTQRVILAGEIRCKGVYVDGQWAAGAREEIEQVVRDVVKRIGYEQEGFHWRTFEF
                     ANHLHGQSAHIAQGVDESGNKDEGAGDQGIMFGYACDETPDLMPATLDYSHKILERLA
                     ADRKSGAAPFLEPDAKSQVTLHYVGGKPVAATAIVVSTQHKPGYDQGEREAELHAYVK
                     RVVGEILPAGFITDATAWHINPTGSFEIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAF
                     SGKDPTKVDRSAAYVARYLAKNVVAAGLATRCTIQLAYAIGVARPLSLYVDTHGTGTV
                     DDAVLERVIAAVAEDRLGGLTPKGIRLGLGLNKPIYGVTAAYGHFGRKAEGDRFPWER
                     TDLVEALRAMLEREAATV"
     gene            complement(1699..3273)
                     /gene="lnt"
                     /locus_tag="VN627_12525"
     CDS             complement(1699..3273)
                     /gene="lnt"
                     /locus_tag="VN627_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HXD04121.1"
                     /translation="MTAPPIRTWRLLAPALLAPAAGAVAACGFQPLMLWPLTLAGVAV
                     LIGLVARARGARQAALAGWLWGVGHFCVGDNWIATAFTYQANMPPSLGWAAVFLLSLY
                     LAVFPALAALAAWFARGRPAALILAFGAAWITSEWLRSWVFTGFAWNPLGVAALGGFG
                     RAGLAQLLPWLGTYALSGLVALIGGCWWLAWQARVERPGPAVLLMLSAGLMLVPQLTG
                     ARGEGSLRYTLVQPGVRQEVLEDPANFERQFATTAQLSAAPTPPGRRLVLWPESGVPD
                     YLRDGYPAYAYDRTTFAGSPALSRRRIGAVIGPGSLLLTGAVDLETRGDEIVAARNVV
                     TAVDAGGAIRGGYAKAHLVPYGEYLPMREWLTPLGLSRLVPGSLDFWPGPGPQTLDLG
                     AWGRAGIQICYEIVFSGRVVDRARRPDYVFNPSNDGWFGAWGPPQHLAQARLRAIEEG
                     LPVLRSTTNGISAVIDADGRVRQFLPRPVAGRLDGLVPPAHAPTLFSRLGNALPLGWA
                     AALAIAALLALRGRRR"
     gene            3257..>3484
                     /locus_tag="VN627_12530"
     CDS             3257..>3484
                     /locus_tag="VN627_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04122.1"
                     /translation="MGGAVIGSRMAAGDGRSPPPAQAGYDNGASAVSNRAVIYRWTGK
                     VRSVVQPSSAATEAAAFGLRPRRLGAGASSSG"
BASE COUNT          563 a         1308 c         1131 g          482 t
ORIGIN      
        1 gaaaagtgga ccgacttcac gatccaccgc gccctgctcc tcggccacat ccccgccctc
       61 tacctcgacc cggccctccc gcgcctgacc cactggcccc acgaggcgga tcaactcgcg
      121 gcctgaggac ccaggtcgcg cgtcacaaac ccttcccctt gatgggggga ggttgggtcg
      181 gggtgaacag cgcaacggca gcaaaccgga gggctggggc tgagcaaagc gcaacgcccg
      241 ccaaatcccc ctgtcctaca tccccctacc catgcgatcc tcgccaacgc tctttgattc
      301 gcgtgaatcc ccccacccgg ggaatagcca acggccgccg ctccatccag aacggcggcc
      361 gtcgtcgtct ggcgcacggc ggaaaattca gtccgcgcaa gccagcatgt atttcttcaa
      421 ctaaaccgtc gccgcttccc gctccagcat cgcccgcagc gcctccacga ggtccgtccg
      481 ttcccacggg aaccggtccc cctcggcctt gcggccgaag tggccatagg ccgcggtcac
      541 cccgtagatc ggcttgttga gcccgaggcc gagccggatg cccttgggcg tcagtccgcc
      601 gagccggtcc tcggccacgg ccgcgatcac ccgctcgagc acggcgtcgt cgaccgtccc
      661 agtcccatgc gtgtcgacat agagcgagag gggccgggcc acgccgatgg cataggccag
      721 ctggatcgtg caccgcgtcg cgaggcccgc ggccaccacg ttcttggcca gataacgcgc
      781 gacataggcg gccgagcggt caaccttggt cgggtccttg ccggagaagg ccccgccccc
      841 gtgcggcgcg gcgccgccgt aggtatcgac gatgatcttg cgcccggtca gcccggcatc
      901 gccgtcgggc ccgccgatct cgaacgagcc ggtcgggttg atgtgccagg ccgtggcatc
      961 ggtgatgaag ccggccggca ggatctcgcc caccacccgc ttcacatagg cgtgcagctc
     1021 ggcctcccgt tcgccctggt cgtagccggg cttgtgctga gtcgagacca cgatcgcggt
     1081 cgccgcgacc ggcttgccgc ccacgtagtg cagggtcacc tggctcttcg catcgggctc
     1141 gagaaacggc gccgcgccgc tcttgcggtc ggccgccagc cgctcgagga tcttgtggct
     1201 gtagtcgagc gtcgccggca tcaggtccgg cgtctcgtcg caggcgtagc cgaacatgat
     1261 gccctggtcg cccgcgccct cgtccttgtt gccgctctcg tccacgccct gcgcaatgtg
     1321 ggcggactgg ccatgcaggt gattggcgaa ttcgaaggtc cgccagtgga aaccctcctg
     1381 ctcgtagccg atccgcttga ccacgtctcg aacgacctgt tcgatttcct ccctggcccc
     1441 cgcagcccac tggccgtcga catagacgcc cttgcagcgg atctcgccgg ccaggatcac
     1501 gcgctgggtc gtggtcagcg tctcgcaggc gatccgggcc tcggggtcct tgcccaggaa
     1561 caggtcgaca atggcatcgc tgatctggtc ggcaaccttg tccggatggc cttcggagac
     1621 gctctcggac gtgaacaggt attcgctgcg cattggactt cgcttccgat ataaagaatt
     1681 ctttatgtgc ctcgctctct aacgccgccg accgcgcagc gcaagcaggg cggcgattgc
     1741 cagtgccgcc gcccagccca gcggcaaggc gttgcccagc ctcgaaaaca gggtcggcgc
     1801 atgggcggga ggcaccagtc cgtccagccg accggccacc gggcgcggga ggaactggcg
     1861 cacgcggcca tcggcatcga tcaccgcgct gatcccgttg gtggtggagc gcaggaccgg
     1921 cagcccctcc tcgatcgccc gcagccgcgc ctgggcgagg tgctgcggcg gcccccaggc
     1981 gccgaaccag ccgtcgttgc tcggattgaa cacgtagtcc ggccgacgcg cccggtcgac
     2041 cacccgtccc gagaacacga tctcatagca gatctggatc cccgcccggc cccaggcacc
     2101 caggtccagc gtttgcggcc ccggccccgg ccagaaatcc agcgaacccg gcaccagccg
     2161 cgacagcccc agcggggtca gccattcgcg catcggcagg tactcgccgt aaggaacgag
     2221 atgggccttg gcatagcccc ctcgaatcgc gccgccagca tccacggcgg ttaccacgtt
     2281 gcgcgccgcc acgatctcgt cgccccgggt ctccagatcg acggcgccgg tcagcagcag
     2341 cgagcccggc ccgatcaccg ccccgatccg ccgccgcgaa agggccgggc tgccggcgaa
     2401 cgtcgtccgg tcataggcat aggcgggata gccgtcgcgc aggtagtccg gcacgcccga
     2461 ttcgggccac agcaccagac gtcgccccgg cggcgtgggc gcagcggaaa gctgcgccgt
     2521 cgtggcgaac tggcgttcga agttggcggg atcctccagc acttcctgcc gcacgcccgg
     2581 ctggacgagg gtgtagcgca aggagccctc gcccctcgcc ccggtcagct gcggcacgag
     2641 catcagcccg gccgagagca tcagcaggac ggccggcccg ggccgctcca cccgcgcctg
     2701 ccaggccagc caccagcacc cgccgatcag ggccaccagc ccggacagcg cataggtgcc
     2761 cagccatggc agcagctggg ccagcccggc gcggccaaat ccgccgagcg ccgccacacc
     2821 cagcgggttc cacgcgaacc cggtgaacac ccagctgcgc agccattcac tggtgatcca
     2881 cgccgcgccg aacgcgagga tcagcgccgc cggcctaccg cgcgcgaacc acgcggccag
     2941 cgccgccagc gccggaaaga ctgccaggta aagcgaaagc aggaacaccg cggcccagcc
     3001 gagcgagggg ggcatgttgg cctggtaggt aaaggccgtg gcgatccagt tgtcgcccac
     3061 gcaaaaatgg ccgacgcccc acagccaccc ggcgagcgcc gcctgccgcg cgccgcgcgc
     3121 ccgcgcgaca agcccgatca gcacggcaac gccggccagc gtcaacggcc acagcatgag
     3181 cggctggaac ccgcaggctg ccactgcacc tgcggccggg gcaagcaggg ccggcgcgag
     3241 caggcgccag gtccggatgg gcggggcagt catcggctca cgcatggcgg cgggtgatgg
     3301 ccgctcgccg ccccccgcgc aagccggata cgacaacggc gcgagtgccg taagcaatcg
     3361 cgccgtcatc tatcgctgga cgggcaaggt ccggtcggtc gttcagccct cgtcggcagc
     3421 aaccgaggcg gcggccttcg gcttgcgccc gcgccgcttg ggggccggag cctcttcgtc
     3481 gggg
//
LOCUS       DATNBV010000528         3229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_930819,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000528 DATNBV010000000
VERSION     DATNBV010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930819"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /locus_tag="VN627_12535"
     CDS             complement(<1..1798)
                     /locus_tag="VN627_12535"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HXD04123.1"
                     /translation="MLRTETFPGSVPGSAYRPGLELWGGAECTVNRTENGFFDQSRAT
                     GHDERDDDIDRLASLRLAALRFPLLWERVAPDDPEQRDWRWSDARLAALSRYGIRPIA
                     GLVHHGSGPRYTSLLDPGFAPGLAAHAAAAARRYPWISEWTPVNEPLTTARFSALYGL
                     WYPHARDERLFWTALVNQIEGVQLAMAEIRKVIPRARLVQTEDLGRTWATARMAQQAA
                     FDNTRRWMTWDLLCGRVVPGHALWERLSGFGLGEHLRRLADTPCPPDVIGVNHYLTSD
                     RFLDHRTAAYPAEVVGGNGREAYADVAAVRALDPAPRGLEMALRDAWERYRLPLAVTE
                     VHNGCTREEQLRWTAQAWTTCQSLRGQGIDVRAMTAWALFGNRGWNTLLTEEGLYEPG
                     AWDVRPEPPRETRLGGLLRRLGSAQPDTDASSLPEAVTGAGWWERPLRLHHDPAPRAA
                     PLRDHRRAPERPRTAAPIVILGATGTLGRAFARACHLRNLPCRLIGRRELDLARPDLM
                     IEALRALGPWAVINAAGWVRVDDAEGDPQGCMAANAAGAIALAQACDDLALPCISFSS
                     DLVFGADAAEWFVEGDRPAPINVYGRSKSAMEA"
     gene            1896..>3229
                     /locus_tag="VN627_12540"
     CDS             1896..>3229
                     /locus_tag="VN627_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HXD04124.1"
                     /translation="MEGSAAVNGASVNAAAKADRIGTLVCFSHLRWNFVFQRPQHLMT
                     RLARSFRVLFWEEPEFGAETDVPRLDLRPCARSGVLVAVPQLPPGLGAAEITTALRSL
                     LDAQLAGAKGPLVRWYYTPMMLPFSDHLRADCTVYDCMDELANFRFAPPDLLPNEARL
                     LEQADLVFTGGYSLYDAKRDRHARVYPFPSSVDREHFAAARGAAADPADQQALPHPRL
                     GFFGVIDERIDVALLAHLADARPDWSVVMVGPVVKISEADLPRRPNLHYLGGKDYGEL
                     PAYLGGWDVALMPFAINEATRFISPTKTPEYLAGGRPVVSTPITDVVRHYGEMAGVRI
                     ASSPEEFVAQCEIALALAGTSDWLAEVDDCLAEMGWDETAGRMLALIEAELASARDVH
                     PIASPAIWPAPPHAGYDVMVVGAGFAGSVMAERLATQAGKRVLVVDRRPHVGG"
BASE COUNT          476 a         1052 c         1191 g          510 t
ORIGIN      
        1 cggcttccat ggccgacttg ctgcggccgt aaacgttaat cggggccgga cgatcgcctt
       61 cgacgaacca ttccgccgca tcggccccga agacgaggtc gctcgagaag ctgatgcagg
      121 ggagggcaag gtcgtcgcag gcttgtgcaa gggcgatggc tcctgcggcg ttggccgcca
      181 tgcagccctg cggatcaccc tcggcatcgt cgacccgaac ccagcccgcg gcattgatta
      241 ccgcccacgg gccgagcgcg cgcagcgctt cgatcatcag gtccgggcgg gcgagatcga
      301 gttcgcggcg gccgatcagg cggcagggca ggttgcgcag gtggcaggcg cgggcaaagg
      361 cccggccgag cgtaccggtt gcgccgagga tgacgatagg ggcggcggtg cggggccgct
      421 cgggggcgcg gcgatgatcg cgcaagggcg ccgcgcgcgg cgcggggtcg tggtgcagcc
      481 gcaagggccg ctcccaccag cccgcgccgg tcacggcctc cggaaggctg gaggcgtccg
      541 tgtcgggctg tgcgctgccg agccggcgca gcagcccgcc gaggcgggtt tcgcgcgggg
      601 gctcgggacg cacgtcccac gccccgggtt catagaggcc ctcttcggtg agcaaggtgt
      661 tccacccgcg attgccgaac agggcccagg cggtcatggc ccgcacgtcg atcccctggc
      721 ctcgcagcga ctggcacgtg gtccaggcct gggcggtcca gcgcagctgc tcctcgcggg
      781 tgcaaccgtt gtggacctcg gtcacggcga gcggcaggcg atagcgctcc caggcatcgc
      841 gcagagccat ctccagcccg cgcggggccg gatcgagcgc gcgcacggcg gcgacatcgg
      901 cgtaggcctc gcggccattg ccgccgacga cctcggccgg ataggcggcg gtgcggtgat
      961 cgaggaatcg gtcgctggtg agatagtggt tcacgccgat gacatccggc gggcaggggg
     1021 tatcggccag gcggcgcagg tgttcgccca ggccgaagcc cgacaggcgc tcccacaagg
     1081 catggccggg gactacccgg ccgcagagca gatcccaggt catccagcgg cgggtattgt
     1141 cgaaggcggc ctgctgggcc atccgcgcgg tggcccaggt gcggccgagg tcttcggtct
     1201 ggaccagccg ggcgcgggga atgaccttgc ggatttcagc catggccagc tggacaccct
     1261 cgatctggtt gacaagggcg gtccagaaca gccgctcgtc gcgggcgtgg ggataccaca
     1321 ggccatagag cgcgctgaac cgggccgtgg tcagcggctc gttgaccggg gtccactcgg
     1381 agatccacgg atagcgccgg gccgcggcgg cggcatgggc ggcgaggcca ggcgcgaagc
     1441 cgggatcgag cagcgaagta tagcgcgggc cgctgccgtg atggacgaga ccggcgatag
     1501 ggcgaatgcc atatcggctg agggcggcca gccgcgcgtc actccagcgc cagtcgcgct
     1561 gctctgggtc atcgggcgcc acccgttccc acagcagggg aaagcgcagc gcggcgaggc
     1621 ggagcgaggc caatcggtcg atgtcgtcgt cccgctcgtc atgaccggtg gcgcgggact
     1681 ggtcgaagaa cccgttttcg gtgcgattca ccgtgcattc ggcgccgccc cacagctcca
     1741 gcccggggcg gtatgccgag ccagggacgc ttcccgggaa ggtttcggtt cgcagcatcg
     1801 gtgtctcaca agttgctgat ttaaaagccg tatcggaaca tgtggcggcc tgccgggttc
     1861 cccttgtcgc gaccctcgcg catggaggaa cttggatgga aggctcagcc gcggtcaacg
     1921 gcgcgtctgt caatgcggct gccaaggccg acaggatcgg cactctcgtt tgctttagtc
     1981 accttcgctg gaatttcgtg ttccagcggc cgcagcacct gatgacgcgg ctggcccggt
     2041 cattccgcgt actgttctgg gaagagccgg agttcggtgc ggaaaccgac gtgccgcgcc
     2101 tggacctgcg cccctgcgcg cgcagcgggg tgctggtggc cgtgccgcaa ttgccccctg
     2161 gactgggtgc agccgagata acgacggccc tgcgcagctt gctcgacgcc cagctcgctg
     2221 gcgcgaaggg cccactcgtg cggtggtatt atacgccgat gatgctgccg ttttcggacc
     2281 acctgcgggc ggactgcacg gtttatgact gcatggacga gctggcgaac ttccgcttcg
     2341 ccccacctga tctgctgccc aacgaagcgc gcctgcttga gcaggcggac ctcgtgttca
     2401 ccggcggtta cagtctttac gacgcgaagc gcgaccgtca cgcgcgggtc tatcccttcc
     2461 cgtcgagcgt ggatcgcgag catttcgccg cggcgcgcgg cgcagccgcc gatcccgccg
     2521 accagcaggc cctgccgcac ccgcgactgg ggttctttgg cgtgatcgac gagcggatcg
     2581 acgtggcact gctggcgcac cttgccgatg cgcggccgga ctggtcggtc gtgatggtcg
     2641 gcccggtggt caagatcagc gaggccgatc tgccgcggcg gcccaatctc cactaccttg
     2701 gcggcaagga ctatggcgag ctgccggcct atctcggcgg ctgggatgtg gcgctgatgc
     2761 cctttgccat taacgaggcg acgcggttca tcagtccgac caagaccccg gaatatctcg
     2821 cggggggcag gccggtggtg tccacaccga tcaccgatgt cgtccgccac tatggcgaga
     2881 tggcgggggt gcgcatcgcc agttcgccgg aggagttcgt cgcccagtgc gagatcgccc
     2941 tggccctggc gggcacgtcc gattggttgg cggaggttga cgactgcctc gccgagatgg
     3001 gctgggacga gacggccggg cggatgctcg cgctgatcga ggccgaactg gccagtgccc
     3061 gggatgttca cccaatcgct tctcccgcca tctggccggc tccgccgcac gcgggctatg
     3121 acgtgatggt ggtgggcgcg ggctttgccg gctcggtcat ggccgagcgg ctggcgaccc
     3181 aggccggcaa gcgggtcctg gtggtcgacc gtcggccgca tgtgggcgg
//
LOCUS       DATNBV010000529         5706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2499862,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000529 DATNBV010000000
VERSION     DATNBV010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2499862"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            525..2045
                     /locus_tag="VN627_12545"
     CDS             525..2045
                     /locus_tag="VN627_12545"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HXD04125.1"
                     /translation="MARPPVRRRQRGASSANSASIVPLNQVGETNPSLFANGPDASLN
                     VAVTYRRVEELKLVRRQLRTHNPKQVDQIVSMIRRMGFINPIIVDADLGIVCGVGRYL
                     AAKQIGLALVPVIEVSHLSPAELRAYAIADNKLVLNDSWDDQLLVIELAELMVEALDL
                     PIELTQFDLVEIDNLLQAAVSDEDAGSVEPDESEPISRLGDLFQLGQHRLLCGDSRKE
                     ESYQTLLGDERARCVFSDNPYNIKIAGNASGLGKVKHGDFVMASGEMSQPEFTAFLLE
                     VFTLLARFSVDGAIHFQCMDHRHLHEMIDAGDAAYGGLKTLVVWDKQRAGMGSFYRNQ
                     MELVFVYKVGKAPHTNNFGLGENGRNRSTIWSYPGAKANDLALHPTVKNLAMVMDAIR
                     DVSDRGEIVLDPFSGAGTTLLACERTGRIGRAIELDPKYVDVGIRRWEQATNQDAIHV
                     ATGLTFAELARSRGVELPGDEEEDEAFTEAGDDAEEADDGEGWNAAGAEAADLDDA"
     gene            2117..2719
                     /locus_tag="VN627_12550"
     CDS             2117..2719
                     /locus_tag="VN627_12550"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HXD04126.1"
                     /translation="MTHNPSADGERRLPPVRQRTRAVPSAIPHGDQSGPAATSGLPPK
                     SATKPPRQSPPTGAAAEGSHYVVGYAKPPKSSQFQAGYDPRRPRGRKPGAKNKANTIV
                     AMMESPTPIRTADGKVQTISTAEALARKLRELGLTGQLGAIIKAFELYFKAQPPAPLP
                     PAGGDAALPDTPTELSAADQAMLAWFEGEVAARTRSEGGA"
     gene            2716..3789
                     /locus_tag="VN627_12555"
     CDS             2716..3789
                     /locus_tag="VN627_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04127.1"
                     /translation="MSLPASQVADALCRTRMFAFVMRAFAHMHPDEEPLAPAWYLMAM
                     CHWLERADAGELPRSMIWIQPRTLKSFTVAIAFPCWLLGRNPAAKIMVATYGEALSSD
                     HAEARRRILLSPWYQALFPQTRPAPRGNSGGTLRTTAGGYVRAVSVGGPVTGRGADVI
                     VLDDCMKADARTSEPARNEVKTWFSTTLTSRLNDKRRGVILSIQQRICEDDLPALLEE
                     KGYVCLNLPAVAERDLDVPIGPGVIHRWQRGELLDPVRFPLDVLEQERLNLGPQDYAA
                     QYLQNPVAPGGNLLKMEQFRRFEQPIAREQFDRVIQSWDPAATDLPTSDWSVCTTWGF
                     VAGRYFLLDILRARLDYPNLNVA"
     gene            3786..4205
                     /gene="terL"
                     /locus_tag="VN627_12560"
     CDS             3786..4205
                     /gene="terL"
                     /locus_tag="VN627_12560"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HXD04128.1"
                     /translation="MIAQRKKWNADHVVIERSSNGLALVQQLMADGPFRPMAWPPPGM
                     RQLDKAERLIAQTGQIEEGRVWLPANLEGLDAFLSELRAFPNGRYDDQVDSLTQLLEW
                     SFWNWRRLKEERGANGRLKAPVRGNRPQLPPLPDWIS"
     gene            complement(4701..5276)
                     /locus_tag="VN627_12565"
     CDS             complement(4701..5276)
                     /locus_tag="VN627_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXD04129.1"
                     /translation="MNESGFVDHYGILGVSPDCDDKTLEAAYRHLAKRYHPDHSGTAD
                     VEQFGRVISAYRALKSAEERGEYNLQYARVTGFVFTDVTAAHDKWGEALTDAAAHEKI
                     LSLLYRRRRESTREPGIGHYTLQEELGCSVEAFDFYTWYLKEKGFIQSTPDGALAITI
                     IGVDQVISATRAGTREQLRIAQQTNASGQVG"
     gene            complement(5351..>5706)
                     /locus_tag="VN627_12570"
     CDS             complement(5351..>5706)
                     /locus_tag="VN627_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04130.1"
                     /translation="PICRPEHETALPFDCTNGQTLARNAKPHHRIEGYAHLILRQFED
                     QRGIGNVVTRCHFRRRSPAWAAALQQLCSSFAAALPQGWFLSRLRIIRAIMAKGVKAK
                     GLKVPSAAIPVPQPF"
BASE COUNT         1036 a         1904 c         1765 g         1001 t
ORIGIN      
        1 ggatgcgatg cacccacaga tcgcgccggg accgttcgat cggaaagggc ctgagatagc
       61 cggagcgctg aaaaccgtgc tgttcagagc ctggctgtgg ggcggcagga ttcgaacctg
      121 cgccacctgc cggcgcagac agcgtgctgg ctggccacga ggtctggggc gaaccactct
      181 ctggcgagat ttcccagaaa acccgggatt tgctgggaat tcgcgctgcc cactgggaat
      241 tcccccgctg cgcccgactg cgaccccgca ggttccctgc gtttgcgcgc aaattcccag
      301 tgatacgctt gctgggaatt ccgcttatct gctgggaatt caattgccag cacgcggcca
      361 gctgctgggc tgctaaccag gagcttgtcc cgcccgccct ccatgctata ttgcacgcat
      421 aggcagcgcc tgagccaacg cgttttcgca ccgagctcca ggggcattct gtcgtcatcc
      481 gcatccgcgg ctggcgccct gaatgcttta tcggagcttt ttcaatggcc agacctcccg
      541 tccgccgccg ccagcgcggc gcttcctctg ccaactcagc cagcattgtc ccgctgaacc
      601 aggtaggcga aactaatccc tcgctgtttg ccaatggccc tgacgccagc ctgaacgtcg
      661 cggtcaccta ccgccgcgtc gaggaactca aactcgtccg gcgccagctg cgcacgcaca
      721 atcccaagca ggtcgatcag atcgtgtcga tgatccgccg catgggcttc atcaacccga
      781 tcatcgtcga tgcggacctc ggcatcgtct gcggagtcgg gcgctatctc gcggccaagc
      841 agatcggcct cgccctcgtc ccggtgatcg aggtcagcca tctgtcgcca gccgaactgc
      901 gcgcctatgc gatcgccgac aacaagctgg tgctcaacga cagctgggat gaccagctcc
      961 tggtgatcga gctggccgag ctgatggtcg aggcgctgga cctgccgatc gagctcaccc
     1021 agttcgacct cgtcgagatc gacaatctgc tccaggccgc ggtcagcgac gaggacgccg
     1081 ggtcggtcga gcccgacgag agcgagccga tcagccggct cggcgacctg ttccagctgg
     1141 gccagcaccg gctgttgtgc ggcgactccc gaaaggagga gagttatcaa acgctgctcg
     1201 gggatgaacg ggccagatgt gtgttctcgg acaaccccta caacatcaag atcgccggca
     1261 acgccagcgg actgggcaag gtcaagcacg gcgatttcgt catggcctcg ggcgagatgt
     1321 cgcagccgga gttcaccgcg ttcctgcttg aggtgttcac gctgctcgct cgcttcagtg
     1381 tcgatggcgc catccacttc cagtgcatgg accatcgcca cctgcacgag atgatcgacg
     1441 ccggcgatgc cgcctatggc gggctcaaaa ctctcgttgt gtgggacaag caacgggcgg
     1501 ggatgggctc gttctaccgc aaccagatgg agctggtctt cgtctacaag gttggcaagg
     1561 cgccgcacac caacaatttc gggctcggcg agaacggccg caaccgctcg acgatctggt
     1621 cgtaccccgg cgccaaggcc aacgatctcg cgctccaccc cacagtgaag aatctcgcca
     1681 tggtgatgga tgcgatccgc gatgtcagtg accgcggcga gatcgtcctc gatccgttct
     1741 cgggggcagg caccacactg ctcgcctgcg agcgcaccgg ccggatcggc cgggcgatcg
     1801 agctcgatcc caaatatgtc gatgtcggga tccgccgctg ggagcaagcg accaaccagg
     1861 acgcgatcca tgtggcgacg ggcctgacct ttgccgaact cgcccgctcc cgcggcgtgg
     1921 aactgcccgg agacgaggag gaggacgagg cgttcaccga ggccggcgat gatgcggagg
     1981 aggcggacga cggggagggc tggaacgcgg caggggccga ggcggccgat ctcgatgacg
     2041 cgtgatcaac cctccggccg cgcgcatcgc gcgcggccgc tctcctgcct ccccctgttc
     2101 gatcggactc acgctcatga cccacaatcc ctccgctgac ggcgagcgcc gcttgccgcc
     2161 cgtgcgccag cgcacccgcg ccgtaccttc ggctattccg catggcgacc agtccggacc
     2221 agcggcgacc tccggcctcc cgcccaagtc tgcaaccaag ccgcctcgcc aatcacctcc
     2281 aaccggcgcg gcggcggagg gtagccacta tgtggtgggc tacgccaagc cgcccaagtc
     2341 cagccagttc caggcagggt atgacccgcg ccgaccacgc gggcgtaagc cgggcgcgaa
     2401 gaacaaggcc aataccatcg tggcgatgat ggaaagcccg accccaatcc gcacagccga
     2461 cggcaaggtg cagaccatca gtacggccga agccctcgcc cgcaagctgc gcgagctcgg
     2521 gctcaccggc cagctcggcg cgatcatcaa ggcgttcgag ctctacttca aggcacagcc
     2581 gccggcgccc ctgccgcccg ctggcggtga cgcggcgctg cccgataccc ccaccgagct
     2641 ctcggccgcc gatcaggcga tgctcgcctg gttcgagggt gaggtcgcgg cgcggaccag
     2701 gtcggaggga ggggcatgag cctgccggca tcccaggtcg ccgacgcgct gtgccgcacg
     2761 cggatgttcg ccttcgtgat gcgcgccttc gcccacatgc accccgacga ggagccgctc
     2821 gcgccggcct ggtacctcat ggcgatgtgc cactggctcg agcgggccga cgccggcgag
     2881 ctgccccgct cgatgatctg gatccagccc cgaacactca agtcgttcac cgtggcgatc
     2941 gcctttccct gctggctgct tggccgcaac ccggcggcca agatcatggt cgcgacctac
     3001 ggcgaggcgc tgagcagcga ccacgccgaa gcccggcggc ggatcctgct cagcccgtgg
     3061 taccaggcgc tgttcccgca gacccgcccg gcgccgcgcg gcaatagtgg cggcacgctg
     3121 cgtaccactg ccggcggcta tgtccgggct gtctcggtcg gcggcccggt gaccgggcgc
     3181 ggggctgacg tgatcgtgct cgacgactgc atgaaggcgg atgcgcgcac cagcgagcct
     3241 gcgcgcaatg aggtcaagac ctggttctcg accaccttga cctcgcgcct caacgacaag
     3301 cgccgcgggg tgatcctctc gatccagcag cggatctgcg aggatgacct gcccgccctg
     3361 ctcgaggaga agggctatgt ctgcctcaac cttcctgcgg tcgccgagcg cgatctcgac
     3421 gtgcctatcg ggcccggcgt gatccaccgc tggcagcgcg gcgagctgct tgacccagtg
     3481 cgcttcccgc tcgatgtgct cgagcaggag cgcctcaacc tgggcccgca ggactatgcc
     3541 gcccagtacc tccagaaccc ggtggcaccg ggcggcaacc tcctcaagat ggagcagttc
     3601 cgccggttcg agcagccgat cgcgcgcgag cagtttgacc gggtcatcca gagctgggac
     3661 ccggccgcga ccgatctgcc gaccagtgac tggtcggtct gcaccacctg ggggtttgtc
     3721 gccggccgct acttcctgct cgacatcctg cgggcccggc tcgactatcc caacctaaac
     3781 gtagcgtgat cgcgcagcgc aagaagtgga acgccgacca cgtggtgatc gagcgctcca
     3841 gcaacggcct cgcgctggtc cagcagctga tggccgacgg cccgttccgg cccatggcct
     3901 ggcctccgcc gggcatgcgc cagctcgaca aggccgagcg gctgattgcc cagaccggtc
     3961 agatcgaaga ggggcgggtc tggctgccgg cgaacctcga ggggctcgat gcgttcctct
     4021 ccgagctgcg ggcgtttccc aacggccgct acgacgatca ggtcgacagc ctcacccagc
     4081 tgctcgagtg gtcgttctgg aactggcgcc ggctcaagga ggagcgcggc gccaatgggc
     4141 ggctcaaggc accggtgcgc ggcaaccggc cccagctgcc accgctgccg gactggatca
     4201 gttaggcacg atcccgcgcc agggctctgt cgaagcaaac gcaccgatgc agtgacggct
     4261 ggagtaggga agggcagggg cgtgccgcgc caccgcaaac ctgccaatcc gtttcgctac
     4321 ttcaactcct cgccgcaggt gatccgcctg gttgtgctga tgtacgtgcg attaccgctc
     4381 tcgttggaaa aggtctaaga gccgctgttc aagcgttccg gcagcggtcc gataacgaac
     4441 gcggtgcctt gcgacgccgt acctccgatt ccaggccacg atgaactgct gcacatagac
     4501 atgcatgtca gaaaccgggt gcggtctgca ggggactccc atcgtctgcg ctcagatggc
     4561 aggagtggct tgatcagtcc tcccgctcgg cgtccgcaaa ctccacggac agtatatggg
     4621 cgcaaaaagc agccttgaaa tagaacttaa ccaggtggga tatttttggt caacttggcc
     4681 cccacgggta tgcgtcgctc ctacccaact tggccgctgg cgttggtctg ttgagcgatg
     4741 cgcagctgtt cccgcgttcc agccctagtt gctgagatca cctgatcgac tccgataatc
     4801 gtgatggcta gcgctccatc cggagtggac tggatgaatc ccttctcctt tagataccat
     4861 gtatagaaat cgaaagcctc aaccgagcag ccaagctcct cctgaagcgt gtaatgccct
     4921 atgcctggct ctcgtgtgga ttccctcctc cggcgatata gcagcgacaa gatcttctcg
     4981 tgtgcggctg cgtctgtgag cgcctctccc cacttgtcgt gtgcggccgt gacatcagtg
     5041 aacacaaagc cagtcactcg agcatactgg agattatatt ccccccgttc ttccgcggat
     5101 tttagcgctc tataggctga tattacacgc ccgaattgct ccacatcggc agtccccgag
     5161 tgatccggat gatagcgctt tgccaaatgg cgatatgcag cctcaagcgt cttgtcgtcg
     5221 cagtctgggg aaactcccaa gatgccatag tggtcgacga agccggactc attcattagc
     5281 acaccttacc tcggtggagc gtaatcgcca actccgaacg agtgtttact cggcttttca
     5341 gaatatcctg tcaaaagggc tgcggaaccg ggatagcagc cgaaggcacc ttcaatcctt
     5401 tagccttgac gcctttagcc atgatggcac gaataatccg aagcctcgat aaaaaccaac
     5461 cttgcggcaa agctgctgca aagctgctgc aaagctgctg caaagctgcg gcccatgcgg
     5521 gagatcgacg gcggaaatgg caccgtgtga ccacgttgcc gatcccgcgc tggtcttcaa
     5581 actggcgcaa gatcaggtgt gcatacccct cgatgcgatg gtgcggcttg gcatttcgag
     5641 cgagggtctg cccgttcgtg cagtcgaagg ggagagctgt ttcgtgctcg ggccggcaaa
     5701 tgggat
//
LOCUS       DATNBV010000530         7179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3795756,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000530 DATNBV010000000
VERSION     DATNBV010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3795756"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VN627_12575"
     CDS             complement(<1..429)
                     /locus_tag="VN627_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXD04131.1"
                     /translation="MRRAANPWRNVYAHPCSWDAHYDPLSLPGLLEQAVARWPDRPLV
                     EFLGRRYSYAALHAQARRFAAGLQEIGIGRGDRVGLFLPNVPAYLAAYYGALIAGATV
                     VNFSPLYSPQELEAQVVDSGTRLLVTVDVPALLPTAIKVLR"
     gene            complement(526..1224)
                     /locus_tag="VN627_12580"
     CDS             complement(526..1224)
                     /locus_tag="VN627_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HXD04132.1"
                     /translation="MTQPTPLMPHATASWLVDNTGLTFEQIAEFCGLHILEVQAMADD
                     LASSKYTGRDPVRAGELTMAEIEKGQDDANYRLKMHKAPLTVSRTKGPRYTPVSKRQD
                     KPDGIAWILRNHPEISDAQIGKLIGTTRSTIAAIRDRSHWNISNINPKDPVTLGLCSQ
                     RELDAIVAKSAKRAGIEDDGLDAVRLGDDREALIEELRAEREAQTRLAGEAAQEAEAA
                     AWLEAKRAAEAQGE"
     gene            complement(1335..2336)
                     /locus_tag="VN627_12585"
     CDS             complement(1335..2336)
                     /locus_tag="VN627_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HXD04133.1"
                     /translation="MSDRLPATMTAIAIAAPGGPEVLQPLELPVPRPGPGEVLVKVAH
                     AGVNGPDVAQRKGAYPPPPGASPIPGLEISGQVAALGEGVTALLPGQPVCALVTGGGY
                     AEWCLAKAAHCLPVPAGLSLAEAAALPETLFTVWHNVFQRGYARDGETLLVHGGTSGI
                     GTMAIMLGKLFGLAVIVTCGSPEKCAAALALGADHAIDYRAADFVAEVARITGGHGVD
                     LVLDMVSGDYVPRNLKCLAEDGRHVTIAVQGGAKAELNMAVIMSRRYTLTGSTLRPRS
                     DAFKALLADEIAREVWPLVASGELRPVMDRAFPLREAAAAHEWMAAGAHVGKIVLTV"
     gene            2427..2636
                     /locus_tag="VN627_12590"
     CDS             2427..2636
                     /locus_tag="VN627_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HXD04134.1"
                     /translation="MDDDLPRPKGDSASKLATEPLDSYSQDELDERIAQLEAEIARVR
                     AHQGKAAAHRLAAEAFFKPPAGGSQ"
     gene            2789..5158
                     /gene="clpA"
                     /locus_tag="VN627_12595"
     CDS             2789..5158
                     /gene="clpA"
                     /locus_tag="VN627_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HXD04135.1"
                     /translation="MPSFAQSLEKTLHAALHNAAERSHEYATLEHLLLALIDDPDAAQ
                     VMTACGVDLHDLAEVVKQYLDQEYQSLKTQDKGDPAPTAGFQRVIQRAILHVQSSGKD
                     TVTGANVLVALFSERDSYAVYFLQQQDMSRLDAVSFISHGIGKGGKRMEDRSPKGAEE
                     SQPAPEEKADAKANGKKDSALDQFCVNLNEKALAGKVDPLIGRGPEVDRTIQILCRRS
                     KNNPLYVGDPGVGKTAIAEGLARKIVEGEVPEVLSEAVIYSLDMGALLAGTRYRGDFE
                     ERLKQVVNELEKMPHAVLFIDEIHTVIGAGATSGGAMDASNLLKPALSGGTIRCIGST
                     TYKEFRNHFEKDRALLRRFQKIDVNEPTVEDTIKILKGLRSAFEEHHKVKYTPEAIKT
                     AVELSARYINDRKLPDKAIDVIDEVGAMQMLLPPSRRKKTITAREIEQVIATMARIPP
                     KSVSTDDKKVLEHLDRDLKRVVFGQDNAISVLATAMKLSRAGLRDPDKPIGSFLFSGP
                     TGVGKTEVARQLASIMGIPLVRFDMSEYMERHSVSRLIGAPPGYVGFDQGGLLTDAVD
                     QQPHSVLLLDEIEKAHPDLFNILLQVMDNGRLTDHHGKTVDFRNVVLIMTTNAGASDM
                     ARQGIGFGDVSKADAGDEAVKKMFSPEFRNRLDAIVPFAYLPTAVISRVVDKFILQLE
                     LQLAEQNVHIQFDSDARAWLGERGYDKLYGARPMARLIQDKVKQPLAEELLFGKLAHG
                     GEVHVKVKDGEGGKELAFELTPAPPKLVKRASKARKPSAAPEPSEDGTE"
     gene            5381..6385
                     /locus_tag="VN627_12600"
     CDS             5381..6385
                     /locus_tag="VN627_12600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HXD04136.1"
                     /translation="MAAPFSWLRAHPQGLCIGPANAWIDPARPVDRALVTHGHSDHAR
                     GGHREVIATAPTLAMMDLRYGGAGREVRVAYGESVDLGGGVRASFYPAGHVLGSAQIL
                     LEHAGERIVVTGDYKRRADPTCAPFQPVPCDVLVTEATFGLPVFRHPPISEEVARLLE
                     TLAANPARCVLVGAYALGKAQRLIAELRLAGWHEPIYLHGAMERMCRLYEEFHVELGD
                     LRLVAGMPKAGLAGQIVICPPSALNDRWSRRLPEPITAMASGWMRVRQRARQRNVELP
                     LVISDHADWDELTGTIAELEPQESWITHGREDALIRWCQLTQRRARALELVGYEDEDD
                     "
     gene            6537..>7179
                     /locus_tag="VN627_12605"
     CDS             6537..>7179
                     /locus_tag="VN627_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXD04137.1"
                     /translation="MERFAALLDALVYTRSRNAKLVLIADYLRATPDPDRGWALAALT
                     DGLDFPAVKGGTIRGLLMDRVDPILFQLSRDFVGDTAETASLLWPGPEVAADPPTVSE
                     VVETLAGLTRATAATGLAALLDRLDANGRYALLKLATGAMRIGISARLAKVAFAQAFD
                     VMVEDVEEYWHGQLPPYAALFAWAAAGAPPPSGEDLPLFRPFMLAHPLEEGTSL"
BASE COUNT         1243 a         2393 c         2436 g         1107 t
ORIGIN      
        1 gcgcagcact ttgatcgcgg ttggcagcag ggcgggaacg tcgaccgtga ccagtaggcg
       61 ggtgcccgaa tcgacgacct gggcctccag ctcctgcggg gaatagagcg gcgaaaaatt
      121 gacgaccgtc gccccggcga tcaaggcgcc ataataggct gcgaggtaag cggggacatt
      181 gggcaggaac aggccgaccc gatcgcccct gccgatgccg atctcctgaa gcccggcggc
      241 aaagcggcgg gcctgcgcat gaagtgcggc gtagctgtag cgacggccaa ggaattcgac
      301 gagcgggcgg tccggccagc gggccacggc ctgttcgagc agccccggca gcgacaacgg
      361 atcatagtgc gcatcccagc tgcaggggtg cgcatagacg ttacgccaag gattggccgc
      421 cctcctcatg ctcatgccag gcaaagtgga tgctgcgggc aggcaaagca aggggcccgg
      481 aagcgaagtg cttccgggcc ccttgctgca ggcaatggca aaagcttact cgccctgggc
      541 ttccgcggcg cgcttggctt cgagccaggc ggcggcctcg gcttcctggg cggcttcacc
      601 ggcaaggcgg gtctgggctt cgcgctcggc ccgcagttcc tcgatcagcg cctcgcgatc
      661 gtcgccgagg cggacagcgt cgagcccgtc atcctcgatg ccggcgcgct tcgccgactt
      721 ggcgacgatg gcatcaagct cgcgctgcga gcacaggccc agcgtcaccg gatccttggg
      781 attgatgttc gagatgttcc agtgcgaacg atcgcggatc gccgcgatgg ttgaccgggt
      841 ggtgccgatc agcttgccga tctgcgcatc ggagatttcc ggatggttgc gcaggatcca
      901 ggcgatgccg tcgggcttgt cctggcgctt ggacaccggc gtgtagcggg ggcccttggt
      961 gcggctgacg gtaagcggcg ccttgtgcat cttcaggcga taattcgcgt cgtcctgacc
     1021 cttttcgatc tcggccatgg tcagctcgcc agcgcgcacc ggatcacggc cggtgtactt
     1081 ggatgaggcg agatcatcgg ccatggcctg gacttcgagg atgtgcaggc cgcagaattc
     1141 ggcgatctgc tcgaaggtca ggccggtgtt gtcgacaagc catgacgccg tggcgtgcgg
     1201 catcaacggg gtgggctggg tcacgttggg tgtactccgg aaacaacaag ggccgcccca
     1261 gcggagcggc ctctaccccg ccgatctatg cgcaaattgc ggattcggca agagcaagca
     1321 agccgcgggc cgattcagac cgtcagcacg atcttgccga cgtgggcgcc cgcggccatc
     1381 cattcatgtg cggcggccgc ttcacgcagg ggaaaggcgc ggtccatcac cggccgcagt
     1441 tcgccagagg cgaccagcgg ccagacctcg cgggcgatct catcggccag cagggccttg
     1501 aaggcatcgg agcgcgggcg cagggtcgag ccggtcagcg tgtagcggcg gctcatgatc
     1561 acggccatgt tgagctcggc cttggcgccg ccctggacgg cgatggtgac gtggcggcca
     1621 tcctcggcaa ggcacttgag attgcgcggc acgtagtcgc ccgaaaccat gtcgaggacg
     1681 agatccaccc cgtgcccacc ggtgattcgc gcgacttcag cgacgaaatc cgcggcgcga
     1741 taatcaatcg cgtgatcggc cccgagggcc agagccgccg cgcacttctc cggcgagccg
     1801 caggtgacga tgacggcgag gccgaacagc ttgcccagca tgatcgccat ggtcccgatc
     1861 ccgctggtcc cgccgtggac gagcagggtc tcgccatcgc gggcgtagcc gcgctggaat
     1921 acgttgtgcc agacggtgaa gagcgtttcc ggcagggccg cggcttcggc cagcgaaagg
     1981 cccgcgggca ctggcaggca atgcgccgcc ttggcgaggc accattcggc atagccgccg
     2041 ccggtgacca gcgcgcagac cggctggccc ggaagcagcg cggtgacccc ttcgccgaga
     2101 gcagcgacct gcccggaaat ctccagcccg ggaatcggcg aggcgcccgg cggcgggggg
     2161 taggcgccct tgcgctgggc gacgtcgggc ccgttgaccc cggcatgggc caccttgacc
     2221 agcacctcgc ccgggccggg ccggggcacc ggcaattcca gcggctgcag cacctcgggt
     2281 cctcccggtg cggcaatggc gatggccgtc atggtcgcag gcaggcggtc tgacatggtg
     2341 gttcctgtgg ctaacctcga gcgcgacccc gcggcgttca tgcagggcgt tgacacgaag
     2401 cgcaaggagt ccaatgttgc attgcaatgg acgacgacct gccccgtccg aaaggcgata
     2461 gcgccagcaa gctggcgacc gagccgctcg actcctactc ccaggatgaa ctggacgagc
     2521 ggatcgccca gctggaagcg gagatcgcgc gggtgagggc ccatcagggc aaggccgccg
     2581 cacatcggct ggccgccgaa gcctttttca agccgccggc cggcggctcg caatgacctt
     2641 tgtgccgcct ctcacaatcg cgcaggcggc acctatattg caaccgatcg ggcaggcagg
     2701 gcgttcaact cctgtctacc ttgccgtcct aagcttcgat ttctcctgcg ggacatccag
     2761 tccgagtccc caacgaaagc cagcctgaat gcccagcttc gcccagagcc ttgaaaagac
     2821 gctgcacgcg gcgctccaca atgccgcgga acgcagccac gaatatgcca cgctggagca
     2881 cctgctgctg gcattgatcg acgatcccga cgccgcccag gtgatgaccg cctgcggggt
     2941 ggatcttcac gacctcgccg aggtggtgaa gcagtacctc gaccaggaat accagtcgct
     3001 gaaaacccag gacaagggcg atcccgcccc gaccgcgggg ttccaacggg tgatccagcg
     3061 cgcgatcctc cacgtgcagt cgagcggcaa ggacacggtg accggggcca acgtcctcgt
     3121 tgccttgttc tccgagcgcg attcctacgc ggtctacttc ctccagcagc aggacatgag
     3181 ccggctcgac gcggtgagct tcatcagcca cggcatcggc aagggcggca agcgcatgga
     3241 agaccgcagc cccaagggcg ccgaggaaag ccagcccgcg ccggaagaga aggccgacgc
     3301 caaggccaat ggcaagaagg attccgcgct cgaccagttc tgcgtcaacc tcaacgagaa
     3361 ggcgctcgcc ggcaaggtcg atccgctgat cgggcgcggc ccggaagtgg accgcacgat
     3421 ccagatcctc tgccgccgct cgaagaacaa cccgctttac gtgggcgatc cgggcgtggg
     3481 caagaccgcg atcgcggaag ggctcgcgcg caagatcgtg gaaggcgagg ttcccgaggt
     3541 gctctccgaa gcggtgatct actcgctcga catgggcgcg ctgctcgccg gcacccgtta
     3601 tcgcggcgac ttcgaggagc gcctcaagca ggtggtcaac gagctcgaga agatgccgca
     3661 tgcggtgctg ttcatcgacg agatccacac cgtgatcggt gccggcgcga ccagcggcgg
     3721 ggcgatggat gcctcgaacc tgctcaagcc cgcgctgtcg ggcgggacca tccgctgcat
     3781 cgggtcgacc acctacaagg agttccgcaa ccacttcgag aaggaccgcg ccctgctgcg
     3841 ccggttccag aagatcgacg tgaacgagcc cacggtggag gacacgatca agatcctcaa
     3901 gggtctgcgc agcgccttcg aggagcacca caaggtcaag tacaccccgg aagcgatcaa
     3961 gaccgcggtg gagctttcgg cccgctacat caatgaccgc aagctgccgg acaaggcgat
     4021 cgacgtgatc gacgaggttg gcgcgatgca gatgctgctg ccgccgagcc ggcgcaagaa
     4081 gaccatcacc gcccgcgaga tcgagcaggt catcgccacc atggcgcgca tcccgcccaa
     4141 gtcggtctcg accgatgaca agaaggtgct ggagcatctg gaccgcgacc tgaagcgggt
     4201 ggtcttcggc caggacaatg cgatcagcgt gctggcgacg gcgatgaagc tcagccgggc
     4261 cggcctgcgc gatccggaca agccgatcgg ctcgttcctg ttcagcgggc ccaccggcgt
     4321 cggcaagacc gaggtcgcgc gccagctggc gagcatcatg ggcatcccgc tcgtacggtt
     4381 cgacatgtcg gaatacatgg agcgccactc ggtctcccgc ctgatcggcg cgccgccggg
     4441 ctatgtcggc ttcgaccagg gcggcctgct gaccgacgcg gtcgaccagc agccgcactc
     4501 cgtgctcctg cttgacgaga tcgagaaggc ccaccccgac ctgttcaata tcctcttgca
     4561 ggtgatggac aacgggcggc tgaccgatca ccacggcaag acggtcgact tccgcaatgt
     4621 cgtcctgatc atgaccacca atgccggggc ctccgacatg gcgcgccagg gcatcggctt
     4681 cggcgacgtc tccaaggcgg atgccgggga cgaggcggtc aagaagatgt tctcgccgga
     4741 attccgcaac cgcctcgatg cgatcgtgcc gttcgcctac ctgcccacgg cggtcatcag
     4801 ccgggtggtg gacaagttca tcctccagct ggagctccag ctggccgagc agaacgtcca
     4861 catccagttt gattccgacg cgcgcgcctg gctgggcgag cgtggctacg acaagctcta
     4921 cggcgcgcgg ccgatggcgc ggcttatcca ggacaaggtc aagcagccgc tcgccgaaga
     4981 gctgctgttc ggcaagctgg cgcacggcgg cgaggtccac gtcaaggtca aggacggcga
     5041 gggcggcaag gagctggcct tcgagctgac cccagccccg cccaagctgg tcaagcgcgc
     5101 gagcaaggcc aggaagccca gcgccgcgcc tgagccgagc gaggacggca cagagtaagt
     5161 tacgatttag ctagactaag gccctcggcg atgccggggg ccttttcttt tgtcactccg
     5221 atccgcttgc ccacccctga cccctcccgc ctgcgggagg gggaacaccg ctgggacaat
     5281 cgcattgctc acttccctcc cgcaagcggg aggcggtggg cactcgcacg accgcaaagg
     5341 cctctggaac cggctgacgt gccgacccat tatccgtggg atggccgcac ccttttcctg
     5401 gcttcgcgcc cacccgcaag ggctgtgcat cggccctgcg aacgcatgga tcgaccctgc
     5461 acggccggtc gaccgggcac tcgtcacgca tggccattcc gaccacgccc gcggcggcca
     5521 tcgcgaggtg atcgcgacgg cgccgacgct ggcgatgatg gacctgcgtt atggcggcgc
     5581 ggggcgcgag gtgcgggtgg cctatggcga gagcgtggac ctgggcgggg gggtgcgggc
     5641 ctcgttctat ccggccgggc acgtgcttgg ctcggcccag atcctgctgg aacatgcggg
     5701 tgagcggatc gtggtcaccg gggattacaa gcgccgggcc gatcccacct gcgccccgtt
     5761 tcaaccggtg ccgtgtgacg tgctggtgac cgaggcgacc ttcggcctgc cggtgttccg
     5821 ccatcctccg atcagcgagg aagtggcccg gctgctggag accctggcgg ccaatccggc
     5881 gcgctgcgtg ctggtggggg cctatgcctt gggcaaggcg cagcggttga ttgccgaact
     5941 gcggctcgcc gggtggcacg agccgatcta cctgcacggt gcgatggagc ggatgtgccg
     6001 cctttatgaa gagttccacg tggaactcgg cgacctccgg ctggtcgccg ggatgccgaa
     6061 agctgggctc gccggccaga tcgtgatctg tccgccctcg gcgctcaacg atcggtggag
     6121 ccggcgcctg cccgagccga tcacggccat ggcctcgggc tggatgcggg tacgtcagcg
     6181 ggcgcggcag cgcaacgtcg aactgccgct ggtgatttcc gaccacgccg actgggacga
     6241 gctgaccggg acaattgccg aactggagcc gcaggaaagc tggatcaccc acggacgcga
     6301 ggatgcgctg atccgctggt gccagctgac gcagcgccgg gcgagggcgc tggagctggt
     6361 ggggtatgag gacgaggatg actgatgccc gacagatcct ccccaccggt ggggaggtgg
     6421 cagccggcac ggctgacgga gtgggctttc cgaccagcgc ggcgcgccat ggaaatcccc
     6481 cttcaccacc cgccggcacg gacggtcccc ctgcccacca gaggggagga ttttaggtgg
     6541 agcgcttcgc cgccctgctc gacgccctcg tctacacccg cagccgcaat gccaagctgg
     6601 tgctgatcgc cgattacctg cgggccacgc ccgatccgga ccgcggctgg gccctggcag
     6661 cattgaccga cgggcttgat ttccctgcgg tgaaaggcgg caccatccgc ggcctgctga
     6721 tggaccgggt cgatccgatc ctgttccagc tctcgcgcga tttcgtcggc gatacggcgg
     6781 aaaccgccag cctgctctgg ccggggcccg aagtcgccgc cgatccgccc acggtgagcg
     6841 aggtggtcga aaccctcgcc ggcctcaccc gcgccacggc cgccaccggt ctcgccgccc
     6901 tgctcgaccg gctcgatgcc aacggccgct acgccctgct caagctcgcc accggcgcca
     6961 tgcggatcgg catttccgcg cggctggcca aggtcgcctt cgcccaggcg tttgacgtca
     7021 tggtggaaga tgtcgaggaa tactggcacg gccagctccc gccctatgcc gccctgttcg
     7081 cctgggccgc agcgggcgca ccgccgccct cgggcgagga cctgccgctg tttcgcccgt
     7141 tcatgctggc ccatccgctc gaagaaggga ccagcctcg
//
LOCUS       DATNBV010000531         3039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2205621,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000531 DATNBV010000000
VERSION     DATNBV010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2205621"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VN627_12610"
     CDS             complement(<1..579)
                     /locus_tag="VN627_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04138.1"
                     /translation="MALALGAAPASAQLLGGGLGGAVSLPGTMIGGAGDVGVSRVGRE
                     VSTTTGGNIEATRRVRQPAVPRRVSAEGALSSRVAAPAAPLAEATASVRAAVPTAAGA
                     ITSSVTGAAGAARVGTSVAVPTLPNLAVRRTAIIEAGIAPVVYDEIPAYVDRQYVVLQ
                     DELRGTGVNVRKQGQQIVLDLPSDVNFAFDRSD"
     gene            complement(729..1946)
                     /locus_tag="VN627_12615"
     CDS             complement(729..1946)
                     /locus_tag="VN627_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXD04139.1"
                     /translation="MDRIPALALLAGLVLLPSGSAAQIAVPPLGGVLGRLDTTLTDTI
                     GTLDRTVRNADDLVRARTERLGRFVAGNAAAVEYDARRQPARRGELLVIDPTADDLRL
                     AADAGYSIVAQGSIEGLDIPYAELAVPDRMPLSRAESALRRLLPRAEITADHIYFASG
                     AVTEGPARSAGAPRLPRGGTVGVIDGGIGPGGDLIGQAGFAIGAPRPSAHAQAIRSLL
                     AGAGTARLYVADIYGSDPAGGNALAIARALGWMIRQEVSVVSISLVGPANPLVRRAVE
                     LARAKGLVVVAAVGNDGPAAPAAYPASYPGVIAVTGVDRQNRVLFEAGKAAHLDYAAP
                     GADLTAVGLDGRRVALRGTSFAAPLAASRIAAHRVNQSSQLSTLQAVDAEAQVRGPRV
                     GRGILCAGCRSGT"
     gene            2029..2526
                     /locus_tag="VN627_12620"
     CDS             2029..2526
                     /locus_tag="VN627_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXD04140.1"
                     /translation="MIPAQFGDKLVALLPQMRRFARGLARDATDGDDLCQATIERALK
                     SADQWQDGTRVDAWMYRIMRNLWIDEHRARARRGPVLPPDDPAFDGADRGDAQIEARL
                     ELGNIDRALARLPDEQREAVLLVLVEGLAYKEAAEVIGCPMGTLTSRLGRGREALLRM
                     IGEAP"
     gene            2523..>3039
                     /locus_tag="VN627_12625"
     CDS             2523..>3039
                     /locus_tag="VN627_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04141.1"
                     /translation="MTISSDQLGAYVDGQSDAEEAQRIEQAAANDPDLTRRIAGERAL
                     RERLRGHFAPVIAEPIPDAWEAMIRAAAAAEQNSGAAVIDLAEARARRGGSVPPNDRR
                     WGRRAWAGAAIAASMVLGLFMGMQLRGGGPIGLRDGALTAQGSLAQALDTQLAASQGS
                     VRYRVMLTFRGT"
BASE COUNT          495 a         1002 c         1096 g          446 t
ORIGIN      
        1 gtccgacctg tcgaaggcga agttcacgtc actcggcagg tcgaggacaa tctgctggcc
       61 ctgcttgcgc acattgaccc cggtcccgcg cagttcgtcc tgaaggacga cgtactggcg
      121 gtcgacgtag gctggaatct cgtcatacac gacaggggcg atgccagcct cgattatggc
      181 tgtgcggcgg acggcgagat taggcagggt cgggaccgca acgctggtgc cgacgcgcgc
      241 ggcgccagcc gcaccggtga cacttgaggt gatcgctcca gcggcggtcg ggacggccgc
      301 gcggacgctg gccgttgcct cggccagagg cgcagcgggc gcagcgaccc gcgacgacaa
      361 ggctccttca gcgctgaccc gtcgcggcac ggccggctgg cggacgcgcc gcgtcgcctc
      421 gatgttgccg ccagtggtgg tcgagacctc ccggccgacg cgcgacaccc cgacgtctcc
      481 cgcgccgccg atcatggtac cggggaggct gacggcgccg ccaagcccac cgccgagcag
      541 ctgggcgctc gccggcgcgg caccaagcgc cagggccatt gtaaccgcaa gggaagttcc
      601 aaggctgttg ttcgagattg gcatcatgtg tcctcctggt tcgctcccgc caagcctcgt
      661 ggcggcaaac tctgtgtgag gaacgatggc ctcatcggat ttaatccatg ggcttgcgca
      721 aaattgaatc aggttccgct gcggcatccg gcgcagagaa tgccgcggcc cacgcggggc
      781 ccgcgcacct gcgcctcggc atcgactgct tgcaacgtgc tgagctgcga gctctggttc
      841 acgcgatggg cggcgatccg ggaggctgca agcggagctg caaacgacgt cccgcgcagt
      901 gccacccgac gaccatcaag gccgacggcg gtgagatcgg caccgggcgc ggcatagtcg
      961 agatgggcgg ctttgccggc ttcgaacagg acgcggttct gccgatccac cccggtgacg
     1021 gcgatcacgc cgggatagga ggcggggtag gctgccggag cggccggacc gtcgttaccg
     1081 acggcggcaa ccacgaccag tcctttcgca cgagccagct cgaccgcgcg gcggaccagc
     1141 ggattggccg gaccgacgag gctaatcgag acgaccgaca cctcttgccg gatcatccag
     1201 ccgagcgctc gggcaatggc gagggcattg ccgcctgccg gatccgaacc atagatgtcg
     1261 gcgacataga gccgcgctgt acccgcgccc gccagcagcg aacggatcgc ctgggcgtgc
     1321 gcgctggggc ggggggcacc gatggcgaag ccggcctggc caataaggtc gcctccgggg
     1381 ccgatcccgc cgtcgatgac gccgacagtg ccgccccggg gcagcctggg agctccggcg
     1441 ctgcgagccg gtccttcggt gacagcgccg ctcgcaaagt agatgtggtc cgcggtaatc
     1501 tcggcccggg gcaacagtcg gcgcagggcg gactcggcgc ggctcaacgg catccggtcc
     1561 ggcacggcga gctccgcgta ggggatgtcg agcccctcga tcgagccctg cgccacgatc
     1621 gaatagccgg cgtcggctgc gaggcggagg tcgtcggcgg tcgggtcgat taccagcagt
     1681 tcgccgcgac gcgccggctg ccgtcgggcg tcgtactcga ccgcggcggc gttgccggcg
     1741 acaaagcggc cgaggcgttc cgtcctggcc cggacgaggt cgtcggcgtt ccgcacggtc
     1801 cggtccagcg tgccaatagt atcagtcagc gttgtatcca ggcgcccgag cactccgccc
     1861 aatggcggca ccgcgatctg ggctgcggag cccgagggca gcagcaccag gcccgcgagc
     1921 agggccagtg cgggaattct atccatgcga ttgtccctca cgaaaaatgt tgccatccag
     1981 ggatgaaacg acggttggtc ggcgtttcat ccctgacaag gccaagtcat gatccctgcg
     2041 cagttcggag acaagctcgt cgcgctgctg ccacagatgc ggcggttcgc acgcggactg
     2101 gcgcgcgatg cgaccgatgg tgacgacctg tgccaggcaa cgatcgagcg cgcgctcaag
     2161 tctgccgacc agtggcagga cggcacgcgc gttgatgcat ggatgtaccg gattatgcgt
     2221 aacctgtgga tcgacgaaca tcgcgcaagg gcacgtcggg gcccggtcct gcctccggac
     2281 gacccggcct tcgacggcgc cgaccgcggc gatgcgcaga tcgaggcgcg gctggagctg
     2341 ggcaacatcg accgcgcgct ggcccgcctg cctgacgagc agcgcgaggc ggtcctgctc
     2401 gtgctggtcg agggcctggc ctacaaggag gcggcagagg taatcggctg cccgatgggc
     2461 acactgacct cgcgcctggg gcgcggacgc gaggcgctgc tgcgtatgat cggagaagcg
     2521 ccatgacgat cagttccgac cagctcggtg cctatgtcga cgggcagtcc gatgccgaag
     2581 aggcccagcg gatcgagcag gctgcggcaa acgatcccga cctcactcgg cgaattgccg
     2641 gcgagcgggc cttgcgcgag cggctgcgag gccatttcgc gccggtcatc gccgagccga
     2701 ttcccgatgc ctgggaagcc atgatccgag ccgccgcggc ggccgagcag aattccggcg
     2761 cggcggtgat cgatcttgcc gaggcgcgtg cccggcgcgg cggttcggtg ccgccgaacg
     2821 atcgcaggtg gggccggcgt gcctgggcag gggccgccat cgcagcgtcg atggtgctgg
     2881 ggttgttcat gggcatgcaa ctgcgcggtg gcgggccaat cggattgcgg gatggagcgc
     2941 taaccgccca aggctcgctg gcgcaggcgc tcgacactca gctcgccgca tcgcagggaa
     3001 gcgtccggta tcgggtcatg ctgacattcc gcgggacag
//
LOCUS       DATNBV010000532         8297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2856366,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000532 DATNBV010000000
VERSION     DATNBV010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2856366"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..984)
                     /locus_tag="VN627_12630"
     CDS             complement(70..984)
                     /locus_tag="VN627_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXD04142.1"
                     /translation="MDVYLPIANLAVNGLVIVALGALTGLLSGVFGVGGGFLTTPLLI
                     FYGIPPTVAAASAASQVTGASVSGVFAHSRRGGVDYQMGGVLVLGGVIGTGIGALLFR
                     LLSALGQIDTVISVLYVVLLGSIGSFMARDAVREIRAHARGLTLPASKRRHHPLVASL
                     PLRWRFYRSGLYISPLAPLLLGVVTGIATMLMGIGGGFMLVPAMLYILGMSAGVVVGT
                     SLFQIMFVTMATTMMHALTTRAVDMVLAVLLLLGSVTGAQVGARLALSVRPERLRLAL
                     AAIVLVVAFRMALGLTYRPDAVYTVTAL"
     gene            complement(1068..1664)
                     /locus_tag="VN627_12635"
     CDS             complement(1068..1664)
                     /locus_tag="VN627_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HXD04143.1"
                     /translation="MRRRLSHEQSRAVALEAARSLLLESGPQAVTLKSVAGRIGRTHA
                     NLLHHFGSAAELQRELAAEMARSICAEIADAVEARRAGARSRRDVVDLVFDAFGRQGG
                     GALASWMLVNGNRDALHPVLGTIHQLLDRLHPEDGEVVRTTALGLVLMALGDALLGEP
                     LSQSLDLPREAAREEAVRMVTEKMKSIDLAAGWNKEPD"
     gene            1701..2588
                     /locus_tag="VN627_12640"
     CDS             1701..2588
                     /locus_tag="VN627_12640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXD04144.1"
                     /translation="MSAIQPLSLVSRGALGNPSQGATTPAGLEIHVRDLRFGRSEAPQ
                     RWGLAEDPVATAWHTGLSATFPRGEAFFIESVKACREGAPPALAAAIGGFIRQEINHT
                     REHLAFNRLAMATGYDLSRVDARVDQLIGETRGKPPAVNLAITMALEHFTAMFAHEFL
                     ANPRHFAGAASEAAAMWRWHAVEELEHKGVAFDTWRHFTRAWPRRRRWLVRSAMMLLV
                     TRKFLANRVGDALDLLDQDSIHGWRAKARLLHYFVVRPGVLRRIFPAWLAYFRPGFHP
                     WHRDDRALIAAAERDLRPA"
     gene            complement(2605..3192)
                     /locus_tag="VN627_12645"
     CDS             complement(2605..3192)
                     /locus_tag="VN627_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXD04145.1"
                     /translation="MFFRSERLFLRPGWPEDWSDLFARIADERIVRNLARAPWPYGPD
                     HARDFAELAQDRRFPHFFITLPRMAAGGYELIGCIGLHEAEGTGGAAELGYWIAKERW
                     GQGYATEAGRAVVAIARTLGHRRITASHFPDNPASGRVLAKLGFRATGRDEPRFSLAR
                     GERVWASVHVLDIAEARGGGDEGGGREMGSMRRAA"
     gene            complement(3428..3697)
                     /gene="rpmA"
                     /locus_tag="VN627_12650"
     CDS             complement(3428..3697)
                     /gene="rpmA"
                     /locus_tag="VN627_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXD04146.1"
                     /translation="MAHKKAGGSSRNGRDSESKRLGVKKFGGQQVIGGNIIIRQRGTR
                     VYPGVNVGMGKDHTLFALGAGRVRFHDGKLGRKYVSVDAMAEAAE"
     gene            complement(3717..4079)
                     /gene="rplU"
                     /locus_tag="VN627_12655"
     CDS             complement(3717..4079)
                     /gene="rplU"
                     /locus_tag="VN627_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXD04147.1"
                     /translation="MFAVVRTGGKQYRVAAGDKIAVEKLAGEAGDKITLGDVLLAGDA
                     GEIKDAAGVVVSAEIIAQAKSEKVIVFKKRRRHNYRRRNGHRQQLTLLRILAVGGEEK
                     KAAKKAAKSEAAPAAAAE"
     gene            4317..4904
                     /locus_tag="VN627_12660"
     CDS             4317..4904
                     /locus_tag="VN627_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04148.1"
                     /translation="MNRPVSPLPRLVSPRRAAGPATLLVGMALLAGCASTAGYPALTK
                     GANELAAEGRITACGIPVARPAPVPVSPAVLPPQLPLPADLPARLAALADQARAANAD
                     FAARRGKASSLVGAASGSGRGSEAWSVASIALADLSSARSRTAVALADLDQLYVAQRV
                     DGGDGIAIAAVRDQVTAWVADEDAVLADLSGRLES"
     gene            complement(4927..5352)
                     /locus_tag="VN627_12665"
     CDS             complement(4927..5352)
                     /locus_tag="VN627_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04149.1"
                     /translation="MSQHASHAELTAVATVSTAPSSHKVDRTFELPAGLYAITVGCYL
                     GFLGLMSVLFMNGELALPMIAFVVSIVGGFGLGYKWVAMNPDNDARTLTMGQFANRGV
                     QTLSGKLTASEASAQVLILPVLLMCWGVAIAVIYAVVGH"
     gene            complement(5461..6477)
                     /gene="hemH"
                     /locus_tag="VN627_12670"
     CDS             complement(5461..6477)
                     /gene="hemH"
                     /locus_tag="VN627_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HXD04150.1"
                     /translation="MTKPADHPAVRTGGIGVLLVNLGTPDAPTPAAVKRYLAEFLSDR
                     RVVEIPPLVWQPILRGIILNTRPKKSAHAYGQVWGPEGSPLAAITRAQAEALQARLGS
                     DLRVRWAMRYGNPAMGAELQALKDAGCDRILVAPLYPQYSGATTASAMDGLAAALKGM
                     RWQPAVRTLPPYYDDALHIAALKDDIARQLSALPFRPEVLLLSFHGMPERTLHLGDPY
                     HCQCRKTARLLAEALAGEWGDLRVETSFQSRFGRAKWLEPATDAVLAAEAARGTRRLA
                     IAAPGFSAECLETREELAIRGREQFLAAGGQDYAALDCLNAGEAGMAMLEALVRRELA
                     GWIN"
     gene            complement(6477..>8297)
                     /locus_tag="VN627_12675"
     CDS             complement(6477..>8297)
                     /locus_tag="VN627_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXD04151.1"
                     /translation="HAAAGARELLCRAAARRWDVDWSECKAANGFVIHGNQRAGFGDL
                     AEDAAEHDLPGDPQLGPAPAEPEGNARTAWPRIDLPPLAEGSARFAGDVRLDGMVYAA
                     IRHGPVGESVALGTVDENKAAGVKLVKGPDWLAAVASTWWAADQALGRIAPEFKVSHP
                     VDGGQIEAALDKAVKKARTERLVTVGDPEGAMGEGAPSLVARYDIAPALHATLETAAC
                     TARFADGRLELWLATQAPETSRRAAARALGLNPRDVVLYPMPAGGSFDARLETPHAVE
                     AALIAREVGKPVQLIWSRWQEHLADRPRAPAAAVLWARTREAADGPNAGGGGEVISWR
                     TRIATPAAAREFGRRFFGGDSPAQALIASAGDADAMALAGAVPPYALANFALDQVPVV
                     TGIPAGRMRGNADAMTAFFTESFVDELARAAKREPLSYRIEMLGGDVRLAECLQHCAT
                     LADWDAGADASGQGLACHRMGEGLRAGRIAVIATARQDEAGVKVEKISAVADIGRIVN
                     RDIARQQIEGGLVFGLGLTLGASTGWARGLPTAERLSQLSLPVLGNTPEIAVEFIESS
                     EPPVDPGELGVVAVAPAVANALHSATGVRFRKLPLFAEEV"
BASE COUNT         1324 a         3100 c         2619 g         1254 t
ORIGIN      
        1 atcggatcgc gcgccgcacc caggaaaacc aggcacagga gggcgaacag ccgcttcacc
       61 gcccgcttct cacaatgccg tgaccgtata gactgcgtcc ggccggtagg tcagcccgag
      121 cgccatgcgg aaagccacga ccagcacgat cgccgcgagg gccagccgca gccgttccgg
      181 ccgcaccgac agggccagcc gtgcgcccac ctgcgctccc gtaaccgaac ccagcagcaa
      241 caggacggcg agcaccatgt cgaccgcgcg cgtggtcagc gcgtgcatca tcgtcgtcgc
      301 catggtcacg aacatgatct ggaacagcga ggtgcccacc accacgcccg cactcatccc
      361 caggatatag agcatggccg gcaccagcat gaacccgccg ccgattccca tcagcatggt
      421 ggcaatgccg gtgaccacac ccagcagcag cggtgccagc ggagagatgt acagccccga
      481 gcggtagaac cgccaccgca gcggcaggct cgcgaccagc ggatgatggc ggcgcttgct
      541 ggcgggaagc gtaaggcctc tggcatgagc gcgaatctcg cgcacagcgt cgcgcgccat
      601 gaaagacccg atcgaaccca ggagaaccac gtacagtacg ctgatcacgg tatcgatctg
      661 gccaagtgcg ctcagcaggc ggaacagcag ggccccgatg ccggtcccga tcaccccgcc
      721 caacaccagc acgccgccca tctggtaatc caccccgccc cggcgcgaat gggcgaacac
      781 cccggacacg ctggcaccgg tcacctggct ggcggccgaa gcggcggcga cggtcggagg
      841 gataccgtag aagatcagca gcggcgtggt caggaagccg ccccccacgc cgaacacgcc
      901 cgacagcagc cccgtcagcg caccgagcgc gacgatgacc aggccattta ccgcaaggtt
      961 ggcgatggga aggtagacat ccatgacttg cggacgtcag cctaggtcag gcagtggtcc
     1021 gggggaagcc gccggacctt gcaaaactgc ggagaaccgg aggcaagtca gtccggctcc
     1081 ttgttccacc cggcggcaag gtcgattgac ttcattttct cggtcaccat ccgcaccgcc
     1141 tcttcgcgcg cggcttcgcg gggcaggtcc agcgactggc tgagcggctc gcccagcagc
     1201 gcatcgccca gcgccatcaa caccaggccg agcgcggtcg tgcgcaccac ctcgccgtcc
     1261 tcgggatgca gccggtccag caattggtgg atcgtcccga gcaccggatg gagcgcatca
     1321 cggttgccgt ttaccagcat ccatgaggcc agcgcgccgc cgccctgccg cccgaatgca
     1381 tcgaacacga ggtcgacaac atcgcgccgt gaccgtgccc ccgcccggcg tgcctccacg
     1441 gcatcggcga tctcggcgca gatcgagcgg gccatctcgg ccgccagttc gcgctgcaac
     1501 tcggccgccg agccgaaatg atggagcaga ttggcatggg tccggccgat ccgtcccgcg
     1561 acggacttga gggtgaccgc ctgtggcccc gattcgagca gaagactgcg cgctgcttcc
     1621 aaggcaactg ccctgctttg ctcgtgggag aggcgccgcc tcattgactt cttgtcaata
     1681 tttgacacct tcccaatacc atgagtgcaa ttcagccgct cagcttagtc tcgcgtggag
     1741 cgctcggcaa tccgtcgcag ggtgccacca ccccagccgg tctcgaaatt catgtgcgtg
     1801 acctgcgctt tggccggtca gaggccccgc agcgctgggg gctcgcggaa gatcccgtcg
     1861 ccacggcctg gcacaccggc ctgtcggcca cgttcccgcg cggcgaagcc ttcttcatcg
     1921 aatcggtcaa ggcctgccgc gagggcgcac cgcccgcgct cgccgcggcg atcggcgggt
     1981 tcatccggca ggaaatcaac cacactcgcg agcatctcgc cttcaaccgc ctggctatgg
     2041 ccaccggcta tgatctctcg cgcgtcgatg cccgcgtcga tcagctcatt ggcgagaccc
     2101 ggggcaagcc gcctgcggtg aacctggcga tcaccatggc cctcgaacac tttaccgcca
     2161 tgttcgccca cgaatttctc gccaacccgc gccatttcgc cggcgccgcg agtgaggcag
     2221 ccgcgatgtg gcgctggcat gcggtcgagg agctcgagca caagggcgtc gccttcgata
     2281 cctggcgcca cttcacccga gcctggccgc gccggcggcg ctggctggtg cggtcggcga
     2341 tgatgctgct cgtcacccgc aagttcctcg ccaaccgggt gggcgatgca ctcgatctgc
     2401 tggaccagga cagcatccac ggctggcgcg ccaaggctcg cctgctgcac tactttgtgg
     2461 tgcgcccggg cgtgctgcgc cggatcttcc cggcctggct ggcctacttc cgaccgggct
     2521 tccatccctg gcaccgcgac gaccgcgccc tgatcgccgc ggccgagaga gaccttcggc
     2581 cggcctgatc cgcacttggg cggatcaggc agcgcgccgc atcgatccca tctcgcgccc
     2641 accgccttca tcgccgcccc cccgggcttc ggcgatgtcg agcacgtgaa ccgaggccca
     2701 cacccgctca ccgcgagcga gcgagaagcg cggctcgtcc cggccagtgg cgcgaaagcc
     2761 aagcttggca aggacccggc ccgaggccgg gttgtccggg aaatggcttg cggtgatccg
     2821 ccggtggcca agcgtgcgcg ctatggcgac gaccgcgcgg cccgcctccg tggcatagcc
     2881 ctggccccag cgctccttcg cgatccagta gcccagttcc gccgcgccgc cggtgccttc
     2941 cgcctcatgc aggccgatgc agccgatcag ctcgtagcca cccgccgcca tccgcgggag
     3001 cgtgatgaaa aagtgcggaa agcgccggtc ctgcgcaagc tcggcaaagt cgcgcgcatg
     3061 gtccgggcca tagggccagg gcgcgcgggc aaggttgcgc acgatccgct cgtcggcgat
     3121 ccgggcgaac aggtcactcc agtcctccgg ccagccgggc cgcagaaaca ggcgttcgct
     3181 gcgaaagaac atgggtgttc tcctctctct cccgcccctc gaccgcactg gtatgccggc
     3241 aagatgacag gtgtgtggcg gttcgcaggc acggcgctcg ggccatcgat cctgcggggc
     3301 gtctcgaggg agaatcaaaa aagggagacg ggcccggggc ccctctccct ctggttctgc
     3361 cggatcagcc aagcggcagg tccggcgggg gccatcccgt caggatggcc cgttatcgac
     3421 catccgatta ttcggcggct tccgccatgg cgtcgaccga gacgtacttg cggccgagct
     3481 tgccatcatg gaagcgcacg cgcccggcgc cgagggcgaa cagggtatgg tccttgccca
     3541 tgcccacgtt cacgcccggg tacacgcggg tgccgcgctg gcgaatgatg atgttgccgc
     3601 cgatgacctg ctggccaccg aacttcttca cgccaaggcg cttcgactcg gaatcgcgac
     3661 cgttacgcga cgagccgcct gctttcttgt gtgccatctc gaactactcc gaaatcttat
     3721 tcagcggccg cggcgggcgc ggcttcagac ttggcggcct tcttggcggc cttcttttcc
     3781 tcgccaccaa cggcgaggat gcgcagcagg gtgagctgct ggcggtggcc gttgcggcgg
     3841 cgatagttgt gccggcgacg cttcttgaag acgatcacct tctcgctctt ggcctgggcg
     3901 atgatttcgg ccgagaccac gacgcccgcg gcgtccttga tctccccggc gtcacccgcc
     3961 agcaggacat cgcccagcgt gatcttgtca ccggcctcac cagccagctt ctcgaccgcg
     4021 atcttgtctc cggcggcaac ccggtattgc ttgccgcccg tgcgcacgac tgcgaacatg
     4081 gtaatcggtt ccaatccaaa cttgcttcta ccgccccgcc gtcccggcac cctgccggct
     4141 agtggcgacg cgccgactcc ccgcccgaag gcaggagccg gaaagaaggt gcgcccctag
     4201 tccaagcgtc ccggcctgtc aacacgccgg cggccccaaa aggccagcaa tcgcggggta
     4261 acgcgcgggt aatgcgcggg ctttcgttcg ctggcccggc gttctatgcc tgcgccatga
     4321 accggcctgt ttctccgctg ccccgcctcg tttccccgcg ccgcgcggcc gggccggcaa
     4381 ccctcctcgt gggcatggcc ctgctcgccg gctgcgcttc gaccgctggt tatcccgcgc
     4441 tcacgaaggg ggccaacgaa ctcgctgccg aagggcgaat caccgcctgc gggatccccg
     4501 tcgcccggcc cgcgccagtg ccggtaagcc cggcggtgct gccgccgcaa ctgccgctgc
     4561 cggctgactt gcccgcccgc ctcgccgccc tcgccgacca ggcccgcgcg gccaatgccg
     4621 attttgcggc caggcgcggc aaggcgtcca gcctcgtcgg agcagcctcg ggatcggggc
     4681 ggggctcgga agcctggtcg gtggccagca tcgcgcttgc cgacctgtcc tcggcccgca
     4741 gccgcaccgc ggtcgccctc gccgatcttg accagctcta tgtcgcccaa cgtgtcgacg
     4801 gcggcgatgg cattgccatc gccgccgtcc gcgaccaggt gaccgcatgg gtcgccgatg
     4861 aagatgcggt cctggccgat ctgtccgggc gcctggaaag ctgaccaagc cggcgctgcg
     4921 tttaactcag tggcccacca ccgcgtagat caccgcgatg gccacgcccc agcacatcag
     4981 cagcaccggc aggatcaaca cctgggccga agcttccgaa gccgtgagct tgcccgagag
     5041 ggtctgcacc ccgcgattgg caaactggcc catggtcagc gtgcgggcat cgttgtcggg
     5101 gttcatcgcc acccacttgt agccaagccc gaacccgccg acgatcgaga ccacgaaggc
     5161 aatcatcggc agcgccagct cgccgttcat gaacagcacg ctcatcaggc caaggaagcc
     5221 gaggtagcaa ccgaccgtga tcgcatagag cccggcgggc agttcgaagg tgcgatcgac
     5281 cttgtgcgac gatggcgcgg tcgaaaccgt ggcgacggcc gtgagttcgg catggctggc
     5341 atgttgcgac atgatcggcg actccttgga ccctgaaagc cggcagacca tccgtccgcc
     5401 ctgtgcctcc cggttactcc gccgccgccg gcgattcgtt gacctcgatc aagcgtcgcg
     5461 tcagtttatc cacccggcca gttcgcgccg caccagcgcc tcgagcatgg ccatcccggc
     5521 ctcgccggca ttgaggcaat cgagcgcggc atagtcctgc ccgcccgccg ccagaaattg
     5581 ctcgcgcccg cggatcgcca gctcctcgcg cgtctcaagg cattcggcgg agaagccggg
     5641 cgccgcgatc gccagccgcc gggtccctcg cgccgcttcg gccgccagca ccgcatccgt
     5701 tgccggctcg agccatttgg cccgcccgaa gcgcgactgg aagctcgtct ccacccgcag
     5761 gtcgccccac tcgccggcca gcgcctcggc cagcaggcgg gcggtcttgc ggcactggca
     5821 atgatagggg tcgcccagat gcagcgtgcg ctcgggcatg ccgtgaaagc tcagcagcag
     5881 cacttcgggg cggaacggca gggccgacag ttgccgggcg atgtcgtcct tcagcgcggc
     5941 aatgtgcagc gcgtcgtcat agtagggcgg cagcgtgcgc accgcgggct gccagcgcat
     6001 gcccttcagc gccgccgcca gcccatccat ggcgctggcg gtcgtcgccc cggaatattg
     6061 cggatagagc ggcgcgacga ggatccggtc gcacccggcg tccttcagcg cctgtagctc
     6121 cgcgcccatc gccgggttgc cataacgcat ggcccagcgc acccgcaggt ccgatccaag
     6181 gcgcgcctgg agcgcctcgg cctgggccct ggtgatcgcg gctagcggcg agccttccgg
     6241 cccccagacc tgcccatagg catgggcgct cttcttcggc cgggtgttga ggatgatccc
     6301 gcgcaggatc ggctgccaca ccagcggcgg gatctcgacc acgcgccggt cggacagaaa
     6361 ttccgcgaga tagcgcttca ccgcggccgg ggtcggcgca tcgggcgtac caagattgac
     6421 cagcagcacg ccgatcccgc cggtccgcac tgctggatgg tcggcaggct tcgtcatcac
     6481 acttcctccg cgaacagcgg cagcttgcga aaccgcacgc cggtcgccga atggagcgca
     6541 ttggccacgg ccggggccac ggccaccacg cccagctcgc ccggatcaac cggcggctcg
     6601 ctgctctcga tgaattccac cgcgatttcc ggggtattgc ccagcaccgg cagcgagagc
     6661 tgtgaaagcc gctctgccgt cggcagcccg cgcgcccagc cggtgctcgc gcccagcgtc
     6721 agacccaggc caaagaccag cccgccctcg atctgctggc gggcaatgtc gcggttcacg
     6781 attcggccga tatcggcgac cgcgctgatc ttctccacct tgacccccgc ctcgtcctgc
     6841 cgcgccgtcg caatcaccgc aatccgcccc gcgcgcaaac cctcgcccat gcggtggcag
     6901 gccagcccct gcccgctggc atcggcgccc gcatcccagt ccgccagggt ggcgcagtgc
     6961 tgcaggcatt cggccagccg cacatccccg cccagcatct cgatgcggta ggagagcggc
     7021 tcgcgcttcg ccgcgcgggc aagttcgtcc acgaaacttt cggtgaagaa ggcggtcatc
     7081 gcgtcggcat tgccccgcat ccgccctgcc gggatacctg tcaccacggg cacctggtcc
     7141 agcgcgaaat tcgccagcgc atagggcggc acagcgcccg ccagcgccat ggcatcggcg
     7201 tcccccgcgc tggcgatcag cgcctgtgcc gggctgtcgc cgccaaagaa gcgccggcca
     7261 aactcgcgcg ccgccgccgg cgtggcgatc cgcgtgcgcc agctgatcac ctcgccgccg
     7321 ccgccggcat tgggcccgtc tgccgcctcg cgcgtccggg cccacagcac cgcggcggca
     7381 ggcgctctcg gcctgtccgc cagatgctcc tgccagcgcg accagatcag ctgaaccggc
     7441 ttgcccacct cgcgcgcaat cagcgccgcc tccaccgcat ggggcgtttc cagccgcgca
     7501 tcgaagctgc cgcccgccgg catcggataa agcacaacgt cgcgcgggtt cagccccagc
     7561 gccctggccg ccgcgcggcg gctggtctcg ggcgcttgcg tcgccagcca cagctcgagc
     7621 cgcccatcgg cgaagcgcgc ggtgcaggcc gccgtctcga gcgtcgcatg cagcgccggg
     7681 gcgatatcgt agcgggcaac caggctcggc gctccctcgc ccatggcccc ttccggatcg
     7741 ccaacggtca ccagccgctc ggtccgggcc ttcttcactg ccttgtccag cgcggcctcg
     7801 atctgaccgc catcaaccgg gtggctgacc ttgaactccg gcgcaattcg ccccagcgcc
     7861 tggtccgccg cccaccaggt ggatgccacc gccgccagcc aatccggtcc cttcaccagc
     7921 ttcacaccgg ctgccttgtt ctcatcaacc gtgcccagcg ccacgctttc gcccaccggc
     7981 ccatgacgga tcgcggcata gaccataccg tccagccgca cgtcgcccgc aaatcgggcc
     8041 gagccctcgg ccaagggagg caagtcaatc cggggccatg cggtccgcgc attgccctcg
     8101 ggttcggcgg gcgctgggcc cagctgcgga tctccgggca agtcatgctc cgcggcgtcc
     8161 tcggccaggt cgccgaaccc ggcgcgctgg ttgccatgga tcacgaaacc gttcgccgcc
     8221 ttgcactcgc tccagtcgac atcccagcgg cgcgccgcag cccggcacag cagttcccgc
     8281 gcccctgccg ccgcatg
//
LOCUS       DATNBV010000533         5523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2207444,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000533 DATNBV010000000
VERSION     DATNBV010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2207444"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            554..1069
                     /locus_tag="VN627_12680"
     CDS             554..1069
                     /locus_tag="VN627_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HXD04152.1"
                     /translation="MEGRRVRFNGQGFSLKGEKDRFVLPPDFRKALRESGTDETGAVR
                     KELCLQLHPDFPCLIAFGLGREAELDDLLDREEENALRRGAPFNRQVRSSQLFGFLKV
                     PFDDSGRFVLPPRFVKGAQIAEQLYFQGAGAEFLIFNPAELMKLPEADWRHAQLACED
                     LAEQALAKAKK"
     gene            1069..2013
                     /gene="rsmH"
                     /locus_tag="VN627_12685"
     CDS             1069..2013
                     /gene="rsmH"
                     /locus_tag="VN627_12685"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HXD04153.1"
                     /translation="MGSAPHVPVLLDEVIAVLAPTPGSLIVDATFGAGGYTRRLLDAG
                     ATVHAFDRDPDAIAAGRLWPETQEQPPRLVLHPRRFSEMVAALAEAGVASVDGVVMDI
                     GVSSMQLDQPHRGFAFAADGPLDMRMAQDGPSAADFLNTADEGEIADVLYTYGEERQS
                     RRVARAIVAARPLGTTGQLAAVVRRALGYKPGQPKDPATRSFQAIRIHVNAELDELRE
                     ALAAAERLLREGGKLAVVSFHSLEDRIVKQFLREASGANPAGSRHLPLQQSAQSPTFA
                     QVTKGIRPSEQELNFNPRARSATLRGATRTGTPARRAA"
     gene            2010..2852
                     /locus_tag="VN627_12690"
     CDS             2010..2852
                     /locus_tag="VN627_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04154.1"
                     /translation="MMLTRSHARSIGWAAILAVCFALTVALSFRVNAVKSQVRLTERR
                     IAQLQVENGLLETEFETRANQQQLTALNEVDFGYQAPTPAQYFESERQLADLGKPRGP
                     DAPPMIRVAANDAPPAGTSVLPAMVNPLTGKTLGADQPKADKPEPKAAAKPAEARLAE
                     RGSATLVERLSRVEQLSHMEKSPKVIDRKVPGLAPRLAAKADPKAKADPKAKSAPKLA
                     KAEIKPAPAKAKAADPKLTSRLAKAAAPKSAAPKSAAKPVIKVALKAPAKAHPGKSDR
                     VAIE"
     gene            2852..4564
                     /locus_tag="VN627_12695"
     CDS             2852..4564
                     /locus_tag="VN627_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXD04155.1"
                     /translation="MTSLTVSTAISSGRVQLVNVRQYTLLVAKLRVWFVVAAFTFIGV
                     VALFRIAWLGVSGDGADSRGMVGLPPRGEIVDRNGVPLARAFPAYSLWYNPKALGDGP
                     ALVKSPAEVAAALVAIFPDEDYAKLKARLASGKPQYLRRRIMPEDANRIHLLGEPALE
                     FPRESERYYPQGSMAAHVLGLIGSNGHGRLGMEAVLDKQLTDPAARGAPAVLSLDSRV
                     QGALEDELLKGMHLSNSKGAGGIVLDVDTGEVLALASLPSYNPNLIDPASVPNIFNRV
                     TNQVYELGSVMKPITVASAIEAGVITDMAKRYPAGPLQIGGFTIHDSHNFGASLNVPE
                     ALIHSSNIVTAQVADQLGGPRLKATMEALGFNRRPDIELPAGFPIWRKGEWPRITTMT
                     VSYGHGLALTPMHLASAYAALVNGGIWRPATLRRLAPNEVPAGKRIWQASTSARMRQL
                     LRMIVSVGTGRKADAAGYRVGGKTGSAEKATGHGYAKSSLITTFAAAFPMDKPRYVII
                     AMMDEPQGTVATSGQRTAAWNAAPVVGRVVPRIGPLLGIMPDATRDVDISDLAPLIGN
                     GDGE"
     gene            4561..>5523
                     /locus_tag="VN627_12700"
     CDS             4561..>5523
                     /locus_tag="VN627_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HXD04156.1"
                     /translation="MNLGALAEAAGIPLPAGAAGTPAGTPAGINVTGFAIDNRKVAPG
                     TVFGAFQGATVNGEDFISAAIQAGAVAIVARPEATVSGAVHIADANPRRLFARLAAPF
                     FAPVPETVVAVTGTNGKTSCVELTRQLWRMAGFSAASIGTLGVTTPDGSQSTGLTTPD
                     IVTFLANMAGLAREGVTHVAYEASSHGLSQYRSEGLRVAAGAFTNLSRDHLDYHATMA
                     DYFAAKMRLFDEVVDGDGAAVLWADDEWSAAAIERARTRGLRLLTVGEKGDTLRLLAR
                     SPSQLGQVLRISHEGAEHTVNLPLIGAYQAANAMVSAGLVLATGG"
BASE COUNT          886 a         2068 c         1720 g          849 t
ORIGIN      
        1 gcctcgcctc gatcatcatg gagcgcgccc gcatggcgga aagcaccatg cccgccgtgg
       61 tcggcgtccc caccgccgcc gccagcccga agagcgatcc tctggccgac atgggggtga
      121 ttccctcccc cgatcccgtc cgcgccaccg ccgccccgcg ctaggctccc gcgctttccc
      181 caaaaccgcc ggtttcccgg tccccctccg gggaaatcgg gggaaggcgc ccgccggcgc
      241 gtcgtaaacc ccacaattcc gccattcccg ccctccgccg cgaggccgcc ctggcgcctc
      301 ccgtggcccg tgccctgtgc cattctcggc aggttatccc caaatccctg cataaaacgg
      361 cccggttgaa cgcattttcc cgcgatttcc cgtaaaattc ccttccctcc cggctcaacc
      421 cgggttacac ctctcctcca tcggaaaggc gaaaggcccg cccatgtgcg ggcgcagcat
      481 ccggtcgccc ttgcggcggt cggaaggggc ttttgcattt ccggtttcgg caaccctggg
      541 cgggggcggg gacttggagg gcaggcgtgt caggttcaac ggacagggct tctcgctgaa
      601 aggcgagaag gaccggttcg tcctgccgcc cgacttccgc aaggccctgc gcgaatccgg
      661 aaccgacgag accggggcgg tccgcaagga gctttgcctc cagctccacc cggacttccc
      721 ctgcctcatc gccttcggcc tcggccgcga agccgagctg gacgatctgc ttgaccgcga
      781 agaggaaaac gccctgcgcc gcggcgcgcc tttcaaccgc caggtccgct cctcgcagct
      841 gttcggcttc ctcaaggtcc cgttcgacga ttcgggccgc ttcgtactcc cgccccgctt
      901 cgtgaagggc gcccagattg ccgagcagct ctatttccag ggcgccggcg cggaattcct
      961 catcttcaac ccggccgaac tgatgaagct gcccgaagcc gattggcgcc acgcccagct
     1021 cgcctgcgaa gatcttgccg agcaggcgct cgccaaggcg aagaagtgat gggctccgca
     1081 ccccacgtcc cggtcctgct cgacgaggtg atcgccgtgc tcgcgcccac cccgggcagc
     1141 ctcatcgtcg atgccacctt cggcgccggc ggctacaccc gccgcctgct cgacgccggg
     1201 gcgaccgtcc acgccttcga ccgcgatccc gatgccatcg ccgccgggcg cctctggccc
     1261 gaaacgcagg agcagccgcc ccgcctcgtg ctccacccgc gccgcttttc cgaaatggtc
     1321 gccgccctcg ccgaagccgg ggtggccagc gtcgatggcg tggtgatgga catcggcgtc
     1381 tcctcgatgc agctcgacca gccccaccgc ggcttcgcct tcgccgccga cggcccgctc
     1441 gacatgcgca tggcccagga cggacccagc gccgccgatt tcctcaacac ggcggacgag
     1501 ggcgagatcg ccgacgtgct ctacacctat ggcgaagagc gccagtcgcg ccgcgtcgcc
     1561 cgcgccatcg ttgctgcccg tccgctgggc acgaccgggc agctcgccgc agtcgtgcgc
     1621 cgcgcgctcg gctacaagcc cggccagccg aaggacccgg cgacccgctc gttccaggcc
     1681 atccgcatcc acgtgaacgc cgaactcgat gaactgcgcg aggcccttgc cgccgccgaa
     1741 cggctcctgc gcgaaggcgg caagctcgcc gtggttagtt tccacagcct ggaagatcgc
     1801 atcgtcaagc agttcctgcg cgaggccagc ggggcgaatc cggcgggttc ccgccacctg
     1861 ccgctccagc agagcgccca gtcgccgact tttgcacagg ttaccaaggg gatacgcccc
     1921 tcggaacagg aactgaactt caatccccgc gcccgctccg ccactttgcg gggcgccacg
     1981 cgaaccggca cgccggcaag gagggcggca tgatgctcac ccgttcccac gcacgatcaa
     2041 tcggctgggc cgcgatcctc gcggtgtgct tcgcgctgac cgtggctctc tcgttcaggg
     2101 tcaatgccgt gaaaagccag gtgcgcctta ccgaacgccg gatcgcccag ctccaggtcg
     2161 agaacggcct gctcgagacc gagttcgaaa cccgcgccaa ccagcagcag ctgaccgcgc
     2221 tcaacgaagt cgatttcggc taccaggcac ccacgccggc ccagtatttc gagagcgagc
     2281 gccaattggc cgatctcggc aagccgcgcg gccccgatgc tccgccgatg atccgcgtgg
     2341 cggcaaacga tgcgccgccc gccggaacgt cggtcctgcc cgccatggtc aatcccttga
     2401 ccggcaagac cctcggcgcc gaccagccca aggccgacaa gcctgaaccg aaagctgccg
     2461 ccaagccggc tgaagcccgc ctcgccgaac gcggctccgc caccctggtc gaacgcctgt
     2521 cgcgggtcga gcagctctcg cacatggaaa agtcgcccaa ggtgatcgac cgcaaggtcc
     2581 ccggcctggc cccgaggctg gccgccaagg ctgatcccaa ggccaaggcc gatcccaagg
     2641 ccaagtccgc tccgaagctc gccaaggccg agatcaagcc ggccccggcc aaggccaagg
     2701 ccgccgatcc caagctgacc tccaggctcg ccaaggccgc cgcgcccaaa tccgccgcgc
     2761 ccaaatccgc cgcaaagccc gtgatcaagg tcgccctcaa ggcgcccgcc aaggcgcatc
     2821 ccggcaagtc tgaccgggtg gccatcgaat aatgacttcg ctcaccgtct cgaccgcgat
     2881 ctcatcggga cgggtgcagc tcgtcaacgt gcgccagtat accctgctgg tcgccaagct
     2941 cagggtgtgg ttcgtcgtcg cggccttcac cttcatcggc gtggtcgccc tgttccggat
     3001 cgcgtggctc ggcgtctcgg gcgacggggc cgatagccgc ggcatggtcg gcctgccgcc
     3061 gcgcggcgag atcgtcgatc gcaacggggt gccgctggcc cgcgccttcc cggcctattc
     3121 gctgtggtac aaccccaagg cgctcggcga tggcccggcc ctggtgaaga gcccggccga
     3181 agtcgccgcc gcgctcgtcg ccatcttccc ggacgaggac tacgccaagc tcaaggccag
     3241 gctcgcctcg ggcaagccgc aatacctgcg ccgccggatc atgccggaag atgccaaccg
     3301 cattcacctg ctcggcgaac cggcgctcga attcccgcgc gagagcgaac gctactatcc
     3361 gcaaggctcg atggccgccc acgtgctcgg cctgatcggg tcgaacgggc atggccggct
     3421 cggcatggag gccgtgctcg acaagcagtt gaccgacccc gcggcgcgcg gtgcacccgc
     3481 cgtgctttcg ctcgacagcc gggtccaggg cgcgctcgag gatgaactgc tcaagggcat
     3541 gcatctctcc aattccaagg gcgcgggcgg gatcgtgctc gatgtcgata ccggtgaagt
     3601 gctggcgctg gcgtcgctgc cctcctacaa ccccaacctg atcgatccgg cttcggtccc
     3661 caacatcttc aaccgggtca ccaaccaggt ttacgaactc ggctcggtga tgaagccgat
     3721 caccgtcgcc agcgcgatcg aagcgggggt gatcaccgac atggccaagc gctatcccgc
     3781 agggccgttg cagatcggcg gcttcaccat tcatgacagc cacaatttcg gggcctcgct
     3841 caacgtcccc gaagccctga tccactcgtc gaacatcgtg actgcccagg ttgcggacca
     3901 gctcggcggc cccaggctca aggcgacaat ggaggcgctc ggcttcaacc ggcgccccga
     3961 tatcgaactg ccggccgggt tcccgatctg gcgcaagggg gaatggccgc gcatcaccac
     4021 gatgaccgtg tcctatggtc atggcctcgc gctcaccccg atgcatctgg cctcggccta
     4081 tgccgcgctg gtcaacggtg ggatctggcg tccggctacc ctgcggcggc tcgccccgaa
     4141 tgaggtgccc gccggcaagc gcatctggca ggccagcacc tcggcccgca tgcgccagtt
     4201 gctgcggatg atcgtctcgg tcggtacggg ccgcaaggct gatgctgccg gctaccgggt
     4261 tggcggcaag accggctcgg cggaaaaagc gaccggacac ggctatgcca agtccagcct
     4321 gatcaccacc ttcgctgcgg ccttcccgat ggacaagccc cgctacgtga tcatcgccat
     4381 gatggacgag ccgcagggca cggtcgccac ctcgggccag cgcaccgccg cctggaacgc
     4441 ggctccggtg gtcggccggg ttgttccgcg gatcgggccg ctgctcggga tcatgcccga
     4501 cgccacgcgc gatgtggaca tttccgatct cgccccgctg atcggcaatg gagacgggga
     4561 atgaacctcg gcgcgctggc cgaggcggcg gggattccgc tgcccgccgg cgccgccgga
     4621 acccctgccg ggactcccgc cggaatcaac gtcaccggct tcgccatcga caatcgcaag
     4681 gtcgcgcccg gcaccgtctt cggggccttc cagggcgcca cggtcaacgg tgaggatttc
     4741 atttccgcgg caattcaggc cggggccgtc gcgatcgtcg cccgcccgga agccacggtc
     4801 agcggcgctg tccacattgc cgatgccaat ccccggcggc tcttcgcccg ccttgccgcg
     4861 ccgttcttcg cgcccgtgcc cgaaacggtg gttgccgtca ccggcaccaa cggcaagacc
     4921 tcctgcgtcg agctgacccg ccaattgtgg cgcatggccg ggttttccgc cgcctcgatc
     4981 ggcacgctcg gcgtcactac gcccgacggc agccagtcca ccgggctgac cacgcccgac
     5041 atcgtcacct tcctcgccaa catggccggc ctcgcccgcg agggcgttac ccatgtcgcc
     5101 tacgaggcga gcagccacgg cctgtcgcaa taccgcagcg aaggactgcg tgtggcggcg
     5161 ggggccttca ccaatctgag ccgcgaccac ctcgactacc atgccaccat ggcggattac
     5221 tttgccgcca agatgcgcct gttcgacgaa gtggtggatg gggatggcgc cgctgtcctc
     5281 tgggccgacg acgaatggtc ggcagcggcg atcgagcggg cgcggacgcg ggggttacgc
     5341 ctgctcacgg tgggcgagaa gggggacacg ctccgccttc tcgcccgctc ccccagccag
     5401 ttgggacagg ttcttcggat ttctcatgaa ggggcggagc acacggtcaa cctgccgctg
     5461 atcggggcct accaggccgc caatgcgatg gtttcggccg gcctggtgct cgccacgggc
     5521 ggc
//
LOCUS       DATNBV010000534         2553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2856481,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000534 DATNBV010000000
VERSION     DATNBV010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2856481"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..2167
                     /locus_tag="VN627_12705"
                     /pseudo
     CDS             337..2167
                     /locus_tag="VN627_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611810.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
     gene            2301..>2553
                     /locus_tag="VN627_12710"
     CDS             2301..>2553
                     /locus_tag="VN627_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04157.1"
                     /translation="MLHSQPIANAYDRINPSISDSAQFIAASIDLPDCVHMAIILGMV
                     ACANICVPIFQRRWVAHNRCCHFAAWHHVVVGTLGQSFLF"
BASE COUNT          482 a          823 c          786 g          462 t
ORIGIN      
        1 cccgccgccc gccgccgatg cgcatgactg catcatggtc aggatcaaac gatcctaccg
       61 catacaagtc tgtggcacga agatgcgccc ccaatggcaa gctcccctcg gatccgcaat
      121 agacagacag acaggcactg accagacacc gaagctatgc aaaaagcccg tctcgccgtt
      181 catcatcgct ccgcgtgctc aggcgtcaag gcgctgcgaa acacaagccc cgctagcctg
      241 acttggccga ttgggtccgg ggctccagaa ttttcgcgtc acaagtaact gatcggaata
      301 ggggcaggag gctgcaatcg gcggccaagc cgtgtagtga agaccaaggt catttcccgg
      361 gtggtggccg cctttataat gcgcagccgt catgtttctc cggcacacca agcgcaagaa
      421 ggacggcaag gagcaccgtt attggagcat cgtggagaat cgccggatcg gcggcggccg
      481 ggtggtgcag cggccgctgc tgtacctggg cgagatcaac gattcgcagg aactggcttg
      541 gcgcaagtca atcgccatcc tggaggaagg ggcactgcag ccgcgcacgc tgtcgctgtt
      601 tccggaggac cgttgcgagg gattgctggc ggacgcctcg atcgttcggt tgcggctaaa
      661 cgaactgcgg ttgtgccggc cgcgccagtg gggcggatgc tggttggcgc tgagcttgtg
      721 gcgcgagctt gagctcgatc gtttctgggg cggacggctg ccgccgagcc gcaagggtac
      781 gcggtgggac cgggtgctgt tgctgctggc gacctaccgg ctgctggcgc cgggcagcga
      841 gtggcggttg catcgcgaat ggtttgagcg cagtgccatg gccgatctgc tcggcgaaga
      901 cctcgggctg gccgaaatcc acaagctgta tcgctgtcac gaccggctgc tcgaacacaa
      961 gcaggccttg ttcgatcatc tggtcgggcg ctggcgcgac ctgttcaaca ttagcttcga
     1021 tgtgctgctc tacgatctga ccagcaccta tttcgaggcc gatccgccgt ttgccgaaag
     1081 cgacaagcga cgccacggct attcgcgcga tcaccggccg gactgcgtgc aggtcgtcat
     1141 cgccttggtg gtgaccccgg aggggttgcc gctggcctac gaggtgctgc ctggcaacac
     1201 cgcggacaac accaccttgc gcgacttcct ggcgcggatc gagcggcagt acggcaaggc
     1261 gcagcgcatc tggttgatgg accgcggcat cccgaccgag gtggtgctgg ccgagatgcg
     1321 gggggccgat cccccggtcc aatacctcgt cggcacgccc aagggccgcc tgacccgatt
     1381 ggagaaggac ctcctcgcga agccctggca gcaggcccgg ccgggggtcc gggtcaagct
     1441 cttgccgcgc gacggcgaac tctatgtttt cgccgacagc gccagccgcg tcgccaagga
     1501 gcgggcaatg cggcgacgac agctgaaatg gttgtgggcg cgcctcaagc agctctcaat
     1561 gatggcgctc agccgcgagg aattgctgat gaagctcggc gctgcccgag cccatgcccc
     1621 agccgcgtgg cgcctggtcg gcatcgcggt agcgcctgag ggcgttacct ttacctacca
     1681 cctcgaccgc aatcggctgc gacgggcgcg ccggcgcgag gggcgctatc tcttgcgcac
     1741 caatctcact gaagacgacc ccgccaagct gtggaatttg tacctgctac tcgtccgcgt
     1801 cgaagaagcc ttcaagaacc ttaagggcga cctcgccatc cggccgatct tccatcaaga
     1861 ccaggcccgg atcgaagcgc atatcttcat cgccttcctg gcctattgcc tgcacgccac
     1921 tctggcgcgg cggctgcatg cactggcgcc ggggctctcc ccgcgcagcg tcatcgagaa
     1981 gttcgcggcg atgcagatga tcgatgtgca cgtcccgacc accgacggcc gcgaattgcg
     2041 gctcacccga tataccgaac ccgatgccga gctgcgctta ctgctcgata agctcaaact
     2101 cgcgttgcca gcgcagtcgc cacccaaaat caccaccgcg caggttgctc cgcaaccccc
     2161 gccgtagtga agacctttga gcgtccgcgc acagaaactc aacgacttaa accactcaaa
     2221 atgcccgaac cggccaagtc aggctagccg agcgcatcca cgacgcaggt gcccactgca
     2281 acaagtaagg ggtatcagcg atgctccaca gccagccgat cgcgaacgct tacgaccgca
     2341 taaacccttc tatctcagac tctgcccagt tcatcgctgc ctcgatcgat ttaccggatt
     2401 gcgtacacat ggcgatcatc ttagggatgg tcgcctgtgc gaacatctgc gtgccgattt
     2461 ttcaacggcg ctgggtagcc cacaatcgat gctgtcactt cgccgcgtgg caccatgttg
     2521 tagttggtac ccttgggcag tccttcctct tca
//
LOCUS       DATNBV010000535         2423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4135073,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000535 DATNBV010000000
VERSION     DATNBV010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4135073"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1166
                     /locus_tag="VN627_12715"
     CDS             291..1166
                     /locus_tag="VN627_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HXD04158.1"
                     /translation="MHAYEHLIGTELGRGRWSWDEETVMLYAVGVGAGLDDPTRDLHL
                     TTENTAGQPLEVLPTFLTQMDGPGKWSRLLGWGDDQYGIAAVHAGQSVTLARPIPTRG
                     TATIINSLDGIYDKGAGALLVVGKRVVLEDSGELLGQGDSIIFVPGKGGFGGPRNPPA
                     QADMSDTAAHEPDMVVSLPIGLNQSLIYRLLGDRNPHTTDPEQARADGFERPAFFGRG
                     TLGVVCRALVRGLCDSDPARMGHVEGRFSNPVYPGDRLDTLIWKTADGAHFQVRANGE
                     RVVFDRGVFRFALAG"
     gene            complement(1190..1849)
                     /locus_tag="VN627_12720"
     CDS             complement(1190..1849)
                     /locus_tag="VN627_12720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXD04159.1"
                     /translation="MTVRQPKIIVFAGSLRAGSYNKALARHAARVAEEAGAEVRLLDL
                     TEYDIPLYNADIYNADAANPYSLVDAGGVCPPYSPPMPAGLLRLREHFRWADGWIVVT
                     PEYNGTFPAVLHNLFEWLTRLAPGERIMELFTFKPVTVLSAAFHGNGKAALEHIKSVL
                     TNLGVLIVPNLVGEAVVYISDENFGPDGALVSPRAQRMAKVTIGRLVDTIVRLKRLDA
                     E"
     gene            2011..>2423
                     /locus_tag="VN627_12725"
     CDS             2011..>2423
                     /locus_tag="VN627_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HXD04160.1"
                     /translation="MRPAEASYAYDRVAFEKTRDAYPEGLPALPEIPGGRYFDEQFYA
                     LEMEHLWRKTWVVAGHISQLPREGSFITFERLGASVVIVRDKTGDIRAFHNVCRHRGA
                     PLVLESSGRVPRFVCPYHSWSFGLDGEVLAITCKE"
BASE COUNT          428 a          759 c          832 g          404 t
ORIGIN      
        1 aagacatcgc cttgtcgcac ccgccggtag gctatcgcgc gcggttcgcg atcggccggg
       61 cactgtagca gcctgatgcg gcggccttgg gagaccaggg ccgccgcatc ggctctgccg
      121 ctccgcacgc atcaccaccc ggacctgagc gatgcgatag aagaaaaatc gccagggggc
      181 acaaatcagc atggacccga tcatcgccgc ctcaagggtt cacggcctgg cgaggtaggc
      241 ttgaccggcg ggacggaggt gcggcaagcc gccggcaaca cggggacacg atgcacgcgt
      301 acgaacacct gatcgggacg gagcttggcc gcggccggtg gagctgggac gaggaaaccg
      361 tgatgctcta cgccgtgggc gtgggcgccg ggctcgacga tccgactcgc gaccttcacc
      421 tgaccaccga gaacacggct ggccagccgc tcgaagtgct accgaccttt ctcacgcaga
      481 tggacggccc cggcaaatgg agccgactgc tgggctgggg cgacgaccag tacggaatcg
      541 cggccgttca cgcggggcaa tccgtcaccc tggcgcggcc gatccccacg cgcgggacgg
      601 ccacgatcat caactcgctg gacgggatct acgacaaggg cgcgggggcc cttctcgtgg
      661 tgggcaagcg agtggtgctg gaagattccg gcgaattgct gggccagggg gacagcatca
      721 tatttgtgcc cggcaagggc ggcttcggcg gtccgcgcaa tccccctgcc caggcggaca
      781 tgagcgacac cgctgcccat gagcccgaca tggtggtgag cctgccgatc gggctcaacc
      841 agtcgctgat ctaccgcctg ctgggcgatc gcaacccgca cacgacagac ccggagcagg
      901 cgagggccga tgggttcgaa cggcccgcat tcttcgggcg cggcactctt ggcgtggttt
      961 gccgggccct ggtgcgcggc ctgtgcgaca gcgatccggc gcgcatgggg cacgtcgaag
     1021 ggcgattctc caacccggtc tatccaggag accggctcga cacgctgatc tggaaaacgg
     1081 ccgacggcgc gcacttccag gtcagggcga atggtgagcg ggtggtattc gatcgggggg
     1141 ttttccggtt cgccctggcc ggatagaggg ccgcgccccc ttgcctgcct cactccgcgt
     1201 cgaggcgttt gaggcgcacg atggtgtcga ccaggcgacc gatggtcacc tttgccatgc
     1261 gctgggcgcg cgggctgacc agtgcgccat cagggccgaa attctcgtcc gagatataga
     1321 ccaccgcctc gcccacgaga ttcggaacga tcaggacccc gaggttggtg aggacggact
     1381 tgatgtgttc aagggcggcc ttgccattgc cgtggaacgc cgccgacagg acggtgacgg
     1441 gcttgaaggt gaagagctcc atgatccgct cgcccggagc caggcgcgtg agccattcga
     1501 acaggttgtg aagcaccgcc gggaaagtcc cgttgtattc gggggtgacg acgatccagc
     1561 catcggccca gcggaagtgt tcgcgcaggc gcaggagccc ggccggcatg ggcgggctgt
     1621 acggcgggca gacaccgccg gcgtcgacca ggctgtaggg attggccgcg tcggcattgt
     1681 agatgtcggc gttgtagagc gggatgtcgt attccgtgag atcgagcaac cgcacttcgg
     1741 caccagcctc ttccgccacc cgcgccgcat ggcgggctag cgccttgttg taggatccgg
     1801 cccgcaggct accggcgaaa acgatgatct tgggctggcg aacggtcatg ctggtctccc
     1861 tgatttttcc gcatccttat gggatcgccg atgggagtcc aacgcaggtt tacacgatgt
     1921 ctggattcgc tgctcgaccc attggtgccg ggcgcttagt ttcatcgcgc accgcaagaa
     1981 acaggcgggt cacccattgg gagaaatgcc atgcgcccgg cagaagccag ctacgcttac
     2041 gacagagttg ccttcgaaaa aacccgtgac gcctaccccg aggggctgcc cgcgttgccg
     2101 gaaattcccg gcggtcgcta cttcgacgaa caattttatg cgctggagat ggagcatctg
     2161 tggcgcaaga cctgggtcgt ggccgggcac atttcccagc tgccgcgcga gggatcgttc
     2221 atcacgttcg agcggctcgg cgccagtgtg gtgatcgtgc gcgacaagac tggtgacatc
     2281 cgcgccttcc acaatgtctg ccgccaccgc ggggcgccgc tggtgctgga aagcagcggg
     2341 cgcgtgcccc ggttcgtctg cccctatcac tcgtggagct tcgggctcga cggcgaggta
     2401 ctggcgatca cctgcaagga aga
//
LOCUS       DATNBV010000536         8229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3799145,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000536 DATNBV010000000
VERSION     DATNBV010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3799145"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VN627_12730"
     CDS             <1..969
                     /locus_tag="VN627_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HXD04161.1"
                     /translation="PVAQVVACGALMKRGIMVKDGSALERLATVDRVLIDKTGTLTLG
                     RPLPDPAVLDALEPDAGRVSLALASHSKHPLSRALVEALAARGIAAAKLTGVLEVPGR
                     GISATWLDATGQATPVALRRPGEATVMAAALDIAGRPTWLIPFSDRLRPGVGQALQAL
                     AGLGVEASILSGDNAGAVGEVARATGLTAEAAADPLAKRDAIARLQGAGRKVLMAGDG
                     LNDGPALAAADASIAPGTASDVGRQAADLVFLGDGLDALPRSIRAARRTMAVVRQNFA
                     LAVGYNVLAVPLAMAGLVTPLIAAAAMSLSSLIVIANSLRLVRAAK"
     gene            966..1121
                     /gene="ccoS"
                     /locus_tag="VN627_12735"
     CDS             966..1121
                     /gene="ccoS"
                     /locus_tag="VN627_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HXD04162.1"
                     /translation="MTGLALLIPIALGMGLFGLAAFFWALRSGQFDDMDGAAARILID
                     EEGDDLP"
     gene            1118..1399
                     /locus_tag="VN627_12740"
     CDS             1118..1399
                     /locus_tag="VN627_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04163.1"
                     /translation="MKRALFGILALVPAAFNVSPATAHGLAVPLCTGDGAARTVTVPM
                     PVRDLPGGDQPGCCAKGCHTGRNGGRDGGRDGGSRKKGPSRQPGRQIDA"
     gene            1437..2750
                     /locus_tag="VN627_12745"
     CDS             1437..2750
                     /locus_tag="VN627_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXD04164.1"
                     /translation="MWPYHPDLLARPVPRYTSFPTAAEFSGAVGAAEQGAALERTSGD
                     VSVYVHIPFCEEICWYCGCNTSRTNREARLTSYLDALHREIAVVGSRLGKDVRVRRIA
                     FGGGSPNAISPLEFVRLTDVLTLHLPLHDPVLSIELDPRTLREDWGMVLRCVGVTRAS
                     LGVQTLSPRLQQAIGRVQPREVIARGTELLRRSGITSLNYDLMYGLPGQTDEELSETL
                     AGAVELGADRVALFGYAHVPHLIPRQKRIDASALPDQDARFRMAWNGWAQLTGAGYHP
                     IGFDHFALPGDALAQAAVAGTLRRNFQGFTEDQAPVLLGLGATAISQFPDLIVQNEKN
                     AGRYRMLLSQERLPGALGIVRSAADQATGLLIEQLLCVGEARVGRDKLVQLAGSLAPF
                     IERGLADAREDWLVIHASGLPYARTIAALFDPYRQDSMRRFSSAV"
     gene            complement(3118..3519)
                     /locus_tag="VN627_12750"
     CDS             complement(3118..3519)
                     /locus_tag="VN627_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXD04165.1"
                     /translation="MLHSDHGASGKAGRERPRVLIMEDEFIVALDLSDMAEDLGFCVD
                     GPFATLAEGAEAVASHCPDAAILDVQLADGEVFPLADALMRLGVPIIFHSGHADNGRL
                     LARYPDARSAGKPCPAELIASHLIQATSRQV"
     gene            complement(3676..5709)
                     /locus_tag="VN627_12755"
     CDS             complement(3676..5709)
                     /locus_tag="VN627_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXD04166.1"
                     /translation="MILSYRPLIAILLQALALVAAVGAAPARAEPTHIAAQLLVEGPA
                     PAGGTATLAFLMKPEAGWHGYWANPGDAGFGMTLNWALPVGVTAGPPRYPVPGTLVVS
                     GLMNHVFEHDYAVLAPLRLPAGLAPGTRLPVRVKAEWLACTDQICVPERAELAAEVLV
                     GPARASDPRFDAWRAKLPAPLGAPARFQLDGKVIRLGIPLPHSLALAKPHVFPAEDGV
                     IAYAAPQEFRRQGDLLVVTLTRSGQAAPAALNGVLRLDEAGNGVEFGAGPGPVPAGGE
                     PLDSGTGASRFALLVLAAIAGGLLLNVMPCVFPILSLKALSLARAGESAAEARTEGLA
                     YTAGVVLACAGLGALLLGLRAAGHELGWAFQLQEPGVVAALLVLAAAITANLAGLFEF
                     AVPGFARAGSANGAFATGLLAAFVATPCTGPFMATAMGAALLLPAVQGMALFVALGLG
                     LALPFLAIALIPALRRALPRPGAWMNTLRRVLAVPMGLTALALAWLAWRLGGQTFAAL
                     AIALTEALLVLLWRLGRAQHGGRPARPFALGAGALAVAAVLALPLTAAPERPASLGGV
                     LPSRPFSEAALAEARASGKPVFAWFTADWCLTCKVNEEVAIERAETAQAFARAGVIVL
                     RGDWTRRDPAIARYLASQGAAGVPLYVWYPAHGGQPQVLPQLLTAGTLSGLTR"
     gene            5811..7493
                     /locus_tag="VN627_12760"
     CDS             5811..7493
                     /locus_tag="VN627_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXD04167.1"
                     /translation="MRRPASFRLALSAALALAQALTLPGSAFAEPAVPAVSNAAPPSP
                     PRLVVAIAVDQFSADLFAQYRQHFTGGLARLTQGAVFPSAYQSHAATETCPGHSTILT
                     GDHPARTGIIGNSWFDPAIGRADKRIYCAEDESDPASTSGQPVVSATQLKVPTLGEWL
                     KRADPRSRNVAVSAKDRAVMMMGGHQIDEAYWYLKGRFVTLKDRAIAPATEAANAAMG
                     AQIARGAPALPAPAWCTPRDRSVQAGRVAVGQGRFALAPGDADGFRVSPRMDAATLDL
                     AGRLVASMSLGRGPAPDVLSVSLSATDYVGHAMGHEGVEMCIQLARLDAGLGTFFARL
                     DAAKLDYVVVLTADHGGVDLPERQVQQALPDAMRVDAGLTPGALGKVLAAELGYSGPG
                     PLVYGEGTFGDFYLARSLPAEQRQRAVASLAAIVARHPQVAAVFTAGELAAAPMPAGD
                     PQDWTLRDRARASFDPARSGDVIVLLRRAVSPIVTPAPGIVATHGSPWDYDRRVPLLF
                     WRRGMHGFEQPAPVETVDIAPSLAALLGLKLPAGTFDGRCLDLDGGADDTCR"
     gene            7692..>8229
                     /locus_tag="VN627_12765"
     CDS             7692..>8229
                     /locus_tag="VN627_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXD04168.1"
                     /translation="MTQDKRDLVILGGGLVGMTLALAAAREGISSHVIERGDPAEITA
                     AGADGRASAISTASWNLFCNIGLKDRLEPLGCPIDAIAVTDQLKPGRIDFRPEPHEGS
                     LGRMYANRDLRLALFGAAKEEPLIAWHTRAEVTARERGPHGVSVTLADGRALAGSLLV
                     AAEGRQSPTRDEAGFGLAK"
BASE COUNT         1235 a         2908 c         2902 g         1184 t
ORIGIN      
        1 ccggtcgccc aagtggtcgc ctgcggggcg ctgatgaagc gcgggatcat ggtcaaggat
       61 ggctcggcac tggagcggct ggccacggtc gaccgggtcc tgatcgacaa gacgggaacg
      121 ctgacgctgg gccggcccct gcccgatccc gccgtgctcg atgcccttga gccggacgcg
      181 gggagagtgt cgctcgcgct cgcctcgcac tccaagcatc cgctgtcgcg cgcgctggtc
      241 gaggcgctcg cggcgcgtgg catcgcggcg gcaaagctca ccggagtgct cgaagtcccg
      301 ggccggggga tcagcgcgac ctggctggat gcgaccgggc aggccacgcc ggtggccctg
      361 cggcgcccgg gcgaggccac ggtgatggcc gcggcgctcg acatcgccgg gcggccgacc
      421 tggctgatcc cgttctccga ccggctgcgg ccgggggtgg gccaagcctt gcaggcgctg
      481 gcgggactcg gggtcgaggc ctcgatcctg tcgggcgaca atgcgggcgc agtgggcgaa
      541 gtggcgcgcg ctaccgggct taccgccgag gccgccgccg atccgctggc caagcgcgac
      601 gcgatcgccc gcctgcaggg cgcgggccgc aaggtgctca tggccggcga tgggctcaat
      661 gacgggcccg ccttggccgc cgcggatgcc tcgatcgccc cgggcacggc gagcgatgta
      721 gggcggcaag cggcagacct cgtgttcctc ggcgacgggc tcgacgccct gccgcgcagt
      781 atccgcgcgg cgcggcggac catggccgtg gttcgccaga acttcgccct ggccgtcggc
      841 tacaatgtgc tcgccgttcc gctggccatg gccggcctgg tcacgccgct gatcgcggcg
      901 gcggcgatga gcctgtcctc gttgatcgtc atcgccaatt cgctgcggct ggtgagggcg
      961 gcgaaatgac cggcctcgct ctcctcatcc ccatagccct cggaatgggc ctgttcggcc
     1021 tcgccgcctt cttctgggcc ctgcgctccg gccagttcga cgatatggac ggcgccgcgg
     1081 cgcgaatcct catcgacgag gaaggagacg acctgccgtg aagcgcgccc tgttcggcat
     1141 tctcgcactg gttccggcgg cgttcaacgt cagcccggcc accgcccatg gccttgccgt
     1201 gccgctgtgc actggcgatg gcgcggcgcg gacggtaacc gtgcccatgc ccgtgcgcga
     1261 ccttccggga ggtgaccagc ccgggtgttg cgccaagggc tgccacaccg gccgaaatgg
     1321 gggccgcgat gggggccgag atggcggatc gcgcaagaaa ggcccctcgc ggcagcccgg
     1381 ccggcaaatt gatgcgtgac aatgatgtcc gggcctgagc gggcgacata actcgcatgt
     1441 ggccctacca ccccgacctg ctggcccggc ccgtgccgcg ctataccagc ttccccactg
     1501 cggccgagtt ctccggcgcg gtgggggcgg cggaacaagg ggcggcgctt gagcggacga
     1561 gcggcgatgt ctcggtttac gtccacattc cgttctgcga ggaaatctgc tggtactgcg
     1621 gctgcaacac cagccgcacc aaccgcgagg cccggctgac cagctacctc gacgcactcc
     1681 atcgcgagat cgcggtggtc ggcagccgcc tcggcaagga cgtgcgcgtc cgccggatcg
     1741 cgttcggcgg cggcagcccc aatgcgatca gcccgctcga attcgtccgc ctgaccgatg
     1801 tcctgaccct gcacctgccg ctgcacgatc cggtgctctc gatcgagctc gatccgcgca
     1861 ccctgcggga ggactggggc atggtcctgc gctgcgtggg cgtcacccgc gccagccttg
     1921 gcgtccagac cctgtcgccc cgcctgcagc aggcgatcgg ccgggtccag ccgcgcgagg
     1981 tgatcgcccg gggcaccgaa ctgctgcgcc ggtccgggat cacctcgctc aactatgacc
     2041 tgatgtatgg cctgccgggc cagaccgacg aggaactgtc cgaaacgctc gccggcgcgg
     2101 tggagcttgg ggccgatcgc gtagccctgt tcggctatgc tcacgtgccg cacctgatcc
     2161 cccgacaaaa gcggatcgat gccagtgccc tgcctgacca ggacgcccgc ttccgcatgg
     2221 cgtggaacgg gtgggctcag ctgaccggag cgggctacca cccgatcggg ttcgaccatt
     2281 tcgccctgcc cggcgatgcc ctcgcccaag cagccgtagc gggaaccctg cgccgcaatt
     2341 tccaggggtt taccgaggat caggcgccgg tcctgctcgg gctcggggca acggcgatca
     2401 gccagtttcc cgacctgatc gtccagaatg agaagaacgc gggtcgctat cgcatgttgc
     2461 tgtcgcagga gcggctgccc ggcgccctcg gcatcgtgcg cagtgcggcc gaccaggcga
     2521 ccggtctgct gatcgagcaa ctgctctgcg tcggcgaggc gcgggtgggg cgggacaagc
     2581 tggtgcagct ggccggctca ttggcgccgt tcatcgagcg cgggctcgcc gacgcccggg
     2641 aggactggct ggtgatccac gccagtggcc tgccctacgc gcggaccatt gccgcgctgt
     2701 tcgaccccta ccgccaggac tcgatgcgcc ggttcagctc ggccgtctga ttgcttcctc
     2761 tccggcaggc aaaaaaaggg acttatgtcc tttgcaaacg caagcgtcgt acacccaccc
     2821 ccaccccctc ccttgaggga ggggagcgag acttggcgca ccccaggtgc gccttagtcg
     2881 cagcggggag ggtttccaaa gctgtcgtgt ttgcaaacga cgcaatcgcc aaaaaaaggg
     2941 gcggccgtcc gatggaggcc gcccctatga aggtggggag atttgcatgt gagacccgga
     3001 ccggtgtcgc gcttggcgga agccagatcg cgtctcgcaa accggaatag gcgaaccagt
     3061 gagtcgccac tttgcgcttg atcaaaaata aataacaagg cgaacttttt tacctgctca
     3121 cacttgccgg ctggtcgcct gaatgaggtg gctggcaatc agctccgctg ggcagggctt
     3181 gcccgcgctg cgggcatcgg gatagcgcgc gagcagccgg ccgttgtccg catggccgga
     3241 atggaagatg atcggcacgc ccaggcgcat cagcgcatcg gccaagggaa acacctcgcc
     3301 gtcggccagc tgcacgtcga gtatcgcggc atcgggacag tggctggcaa cggcctccgc
     3361 gccttcggcc agggtggcga aggggccatc gacgcagaag ccgaggtcct cggccatgtc
     3421 ggaaagatcg agggcgacga tgaattcgtc ttccatgatg agcacgcggg gccgctcgcg
     3481 ccctgccttg ccacttgcac catgatcgct atgcaacatc tggacccgaa acccgtcgtg
     3541 attgttaagc tggaccggcg gccgacggac tccccaatcc gcgtcgccgt gccgcggaag
     3601 gcggccgatt aaattcatca atgcggtaag caggccccgg ttccccgggt taccgacaat
     3661 ggttcggttt ctcggtcagc gggtcaggcc ggacagggtg ccggcggtga gcagttgcgg
     3721 gagcacttgc ggctgtccac cgtgcgcagg gtaccagaca tagagcggaa ccccggccgc
     3781 gccctggctg gcaaggtagc gggcgatggc cggatcccgc cgggtccagt cgccccgaag
     3841 cacaatgact cccgcgcgcg caaaggcctg cgctgtttcc gcccgctcga tcgcgacttc
     3901 ctcgttgacc ttgcaggtca ggcaccagtc ggcggtgaac caggcgaaca ccggcttgcc
     3961 cgaggcgcgc gcctcggcca gcgcggcttc gctgaaggga cggctcggca gtacgccgcc
     4021 caggcttgcc gggcgttcgg gggcagcggt cagcggcagg gcaagcacgg cggcgaccgc
     4081 cagcgcccct gcgccgagcg caaaaggccg cgcagggcgg ccgccatgct gggcccggcc
     4141 aaggcgccag agcaggacaa gcagcgcctc cgtcagcgca atcgccagcg cggcgaaggt
     4201 ctggcctccc agccgccagg ccagccaggc cagcgccagc gcggtcagcc ccatcggcac
     4261 ggcgaggacg cggcgcagcg tgttcatcca ggcgccgggg cgcggcaggg ctcgtcgcag
     4321 cgccgggatc agggcaatgg cgaggaacgg cagggcgagg ccaaggccga gcgccacgaa
     4381 cagcgccatg ccctgcaccg ctggcagcag cagggccgcg cccatggccg tggccatgaa
     4441 cggccccgtg cagggcgtgg cgacgaaggc ggcgagaagc ccggtggcga aggcgccatt
     4501 ggcgctgccc gcgcgggcaa agccgggcac ggcaaattcg aacagtccgg caaggttggc
     4561 ggtaatcgcc gccgccagca ccagcagcgc ggccaccacg cccggttcct gcagctggaa
     4621 ggcccagccg agctcatgcc ccgccgcgcg caagcccagc agcagcgcgc cgagcccggc
     4681 acaggcgagc acgaccccgg cggtataggc caggccttcg gtccgcgctt ccgccgcgct
     4741 ctccccggcg cgggcgagcg agagcgcctt gaggctgagg atcggaaaga cacagggcat
     4801 gacgttgagc agcagcccgc cggcaatggc ggcaaggacc agcagggcaa agcggctggc
     4861 gccggtcccg ctgtcaagcg gctcgccgcc cgctggcacc ggccccgggc cggcgccgaa
     4921 ctccaccccg ttgccggcct cgtcgagccg cagcacaccg ttcagcgcgg cgggcgcggc
     4981 ctggcccgac cgcgtgagag tgaccaccag caggtcgccc tggcggcgga actcctgcgg
     5041 cgcggcatag gcgatcaccc cgtcttcggc gggaaagacg tggggtttgg cgagggcgag
     5101 gctgtggggc agggggatac cgaggcggat caccttgccg tcaagctgga agcgcgccgg
     5161 ggcgccgagc ggcgcgggca acttcgcgcg ccaggcatcg aagcgcgggt ctgacgctcg
     5221 ggccgggcca acgaggactt cggccgccaa ctcggcgcgt tcggggacgc agatctgatc
     5281 agtacaggcc agccattcgg ccttcacgcg gaccggcagc cgggtgcccg gggccagccc
     5341 ggccgggagg cgcaagggag cgagaacggc ataatcgtgc tcgaacacat ggttcatcag
     5401 cccggagacc accagcgtgc caggcaccgg atagcgcggc ggcccggcgg tgacgccgac
     5461 gggtagcgcc cagttcagcg tcatcccgaa gccggcatcg ccggggttcg cccagtagcc
     5521 gtgccagccg gcttccggct tcatcaggaa ggcgagggta gccgtgccgc ccgccggggc
     5581 cggaccttcc accagcagct gcgcggcgat gtgcgtcggc tcggcgcggg cgggggcggc
     5641 acccacggcc gccaccagcg cgagcgcctg caacaggatt gcaatgaggg ggcggtagct
     5701 gaggatcatc gggcgggctg gccttggcgc gaaactgtgg cttgcgcggg gctgttgctc
     5761 ccgcctagcc atgcactgcc gctttgccgg ctgcaattca aggatcgatc atgcgccgac
     5821 ctgcttcctt ccggctggcc ctcagcgccg ccttggccct ggcccaggcg ctgaccctgc
     5881 ccggctccgc cttcgccgag ccggctgtgc ccgccgtcag caatgcggcg ccgccaagtc
     5941 cgccgcgcct cgtcgtcgcc atcgcggtcg accagttttc ggccgacctc ttcgcccagt
     6001 accgtcagca cttcaccggc gggctggccc ggttgacaca gggcgcggtc tttcccagcg
     6061 cgtaccagag ccacgcggcg accgagacct gcccggggca ttcgaccatc ctcaccggcg
     6121 atcacccggc ccgcaccggg atcatcggca actcctggtt cgatcccgcc atcggccggg
     6181 ctgacaagcg gatctactgc gccgaagacg agagcgatcc ggcgagcacc tcgggccagc
     6241 cggtggtttc ggccacgcag ctcaaggtgc cgacgctggg cgaatggctc aagcgggccg
     6301 atccgcgcag ccgcaacgtc gccgtctcgg ccaaggaccg cgccgttatg atgatgggcg
     6361 gccaccagat cgatgaagcc tactggtatc tcaagggccg cttcgtgacg ctgaaagacc
     6421 gggcgatcgc accggcgaca gaggcggcca atgcggccat gggcgcgcag atcgcccgcg
     6481 gcgccccggc gctgcccgcc cctgcctggt gcacgccgcg tgaccggtcg gtccaggccg
     6541 ggcgggtggc cgtggggcag gggcgcttcg ccctggcccc gggagatgcc gatggatttc
     6601 gcgtttcgcc ccggatggat gccgccacgc tcgatcttgc cggacgcctg gtcgcatcga
     6661 tgagcctggg ccgcggcccg gcgcccgacg tgctgtcggt cagcctctcg gccaccgact
     6721 atgtcggcca tgccatgggc catgaagggg tggagatgtg catccagctg gcccggctcg
     6781 atgccgggct ggggaccttt ttcgcccggc tggatgcggc caagctcgac tacgtggtgg
     6841 tgctgaccgc cgatcatggc ggggtcgacc tgcccgaacg gcaggtccag caggccctgc
     6901 cggatgcgat gcgggtcgat gccgggctca cgccgggcgc gctgggcaag gtgctcgccg
     6961 ccgagctggg ctacagcggg cccggtcccc tggtctatgg cgagggcacc tttggcgact
     7021 tctatctcgc gcgcagcctt cccgccgagc agcgccagcg cgcggtcgct tcgctggccg
     7081 ccatcgtcgc ccgccacccg caagtggccg ccgtgttcac cgccggtgaa ctcgccgccg
     7141 ccccgatgcc cgccggcgat ccgcaggact ggaccctgcg cgaccgcgcc cgcgccagct
     7201 tcgatcccgc ccgctcgggc gacgtgatcg tcctgctgcg ccgcgcggtt tccccgatcg
     7261 tcacccccgc gccggggatc gtcgccaccc atggcagccc atgggactat gaccggcggg
     7321 tgccgctgct gttctggcgc cggggcatgc acgggttcga gcagcccgcg ccggtggaaa
     7381 cggtcgacat cgcgccgagc cttgccgcgc tgcttgggct caagctgccg gcgggaacct
     7441 tcgatggccg ctgcctcgat ctcgatggcg gggcggacga tacgtgcagg tagtctaact
     7501 ccaacaccgc aaactacccg tcgcccctgc gtaggcgggg gcccagccca gccccgcgcc
     7561 agagcgccgc tgttacacgt gtgggtaacg tcggcttggg cccccgcctg ccctccgggc
     7621 agtctatcct gagcgcgtcc gcacctgcgg acccttgccc tgcctccgcc cccgggcgta
     7681 aggctgggcc catgactcag gacaagcgcg atctcgtgat cctcggtggt gggctggtgg
     7741 gcatgaccct ggccctggcc gccgcacgcg aggggatttc cagccacgtg atcgaacgcg
     7801 gcgacccggc cgagatcacc gccgccgggg ccgacgggcg ggcttcggcc atctcgaccg
     7861 ccagctggaa cctgttctgc aacatcgggc tcaaggaccg gctggagccg ctcggctgcc
     7921 cgatcgatgc cattgccgtg accgaccagc tgaagccggg gaggatcgac ttccggccag
     7981 agccgcacga gggctcgctc ggccggatgt acgccaaccg tgacctgcgc ctcgccctgt
     8041 tcggcgcggc gaaggaagaa ccgctgatcg cctggcatac gcgggccgaa gtcaccgcgc
     8101 gcgaacgcgg accccacggg gtgagcgtga ccctcgccga tggacgggcg ctcgccggca
     8161 gcctgctggt cgccgccgaa ggccgccagt cgcccacccg tgacgaggcg ggtttcggcc
     8221 tggccaagt
//
LOCUS       DATNBV010000537         2786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3799756,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000537 DATNBV010000000
VERSION     DATNBV010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3799756"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VN627_12770"
     CDS             <1..546
                     /locus_tag="VN627_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXD04169.1"
                     /translation="QTNDDFGMMREVMARRFGRALAEDPDRENGTWPDLVLIDGGKGQ
                     MSAVKETVEELGIEDLPLIAIAKGPHHGREGREVFHFPDGREKTLPVNSPVLFYLQRL
                     RDEVHRYAIGAHRDKRSRAIVASPLDEIPGIGPARKRALLLHFGTAGKVRAASLEDLQ
                     RAPGVSATVAQAIYDFYHPGG"
     gene            649..1923
                     /locus_tag="VN627_12775"
     CDS             649..1923
                     /locus_tag="VN627_12775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD04170.1"
                     /translation="MDELATTGARAEWKAHWRVAVAATAGVSLGAVPAFGLGMFIHPL
                     EQEFGWSRAGISLATTILSSVGVLLGWLSGIMVDRIGARRVGMAGVATFCALLVLLSQ
                     ITADIRTWWAVWLLLSIACLGMQTTVWSKAVNSCFDKGRGVALALMASGTGISTIFLP
                     TLCNFLIGNYGWRIAFIAVGAIYAAVILPLLWRWFYEATDDRPGTRAAGPSRAERQAG
                     LPGWAAREGLRKRQTYQIAIAGMLAGTVCTGYSVHIVSMLTDKGMARDAAAAMLSVLG
                     LLAVISRLVIGRIFDRVPSPLVGIISVSLPVIPGLLLLLLPPTPLVGLLAAASVGLAI
                     GGEYDTVLYMSSRFFGLKAFGTLFGVVATILTAGFAMGPLIAGRIFDLTGDYQLYFIG
                     TVPLTLIAVLLIATLGPYPDHAPADGRTAHPA"
     gene            complement(1959..>2786)
                     /locus_tag="VN627_12780"
     CDS             complement(1959..>2786)
                     /locus_tag="VN627_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HXD04171.1"
                     /translation="LNCNWKVALEAFLEGYHAQTTHRQVNNCDGGAHSICQTFGRHSM
                     YSMYNVGNLGDRSHNAEYLPPFNSDLFEGVTDRRARIALYMQVLNADLQCMFTDHLVE
                     AGRRLPERVEADATSDEMGLALLTLERDIATEAGVDLSHLSLEDFNKLGIDWNMFPNM
                     AMLPTQNGLLVYRARPDGDDPDKCLFDIMPLERYAPGKAPKVTHEFYEDWRDGNYPRI
                     FIQDYENVDEVQRGMKSAGFPGALPNPLAEKVVWNLHRELREMVLGEQQSAERMAAE"
BASE COUNT          416 a          875 c          971 g          524 t
ORIGIN      
        1 cagaccaacg atgacttcgg catgatgcgc gaggtcatgg cccggcggtt cggccgtgcc
       61 ctggccgagg atccggatcg cgagaacggg acctggccgg acctggtgct catcgacggc
      121 ggcaagggcc agatgagcgc ggtgaaggag acagtggagg agctcggcat cgaggacctg
      181 ccgctgatcg ccatcgccaa gggtccgcat cacggacgcg aggggcgcga ggtgttccat
      241 tttcccgacg ggcgcgagaa gacgctgccg gtcaattctc ccgtgctgtt ctatctgcag
      301 cggctgcgcg acgaggtcca ccgctatgcc atcggcgcgc accgcgacaa gcgcagccgg
      361 gcgatcgtgg ccagcccgct tgacgagatt ccgggcattg gtcccgcgcg caagcgagcg
      421 ttgctgctgc acttcgggac ggccgggaag gtccgtgcgg caagccttga ggacctgcag
      481 cgcgcccccg gcgtgagcgc gaccgtggcg caagcgatct acgatttcta tcaccccggc
      541 ggttgaccag ccgggctccg gctgcttcgt tggcgccgac gcagcccaag ctgcgcaggg
      601 tctccggcgc cggaagcgca ggtcagaccg gccgaccggg agacatgcat ggacgagctg
      661 gccacaaccg gcgcgcgggc ggaatggaag gcgcactggc gggttgccgt tgccgcgacg
      721 gccggtgttt cgctcggcgc ggtcccggcc ttcgggcttg gcatgttcat ccacccgctc
      781 gagcaggagt tcggctggag ccgggcggga atcagcctgg cgacgaccat cctttccagc
      841 gtgggcgtgc tgttgggctg gctctcgggt atcatggtcg accggatcgg cgcacggcgc
      901 gtgggcatgg ccggagtcgc gacgttttgc gcgctgcttg tccttctgtc gcagatcacg
      961 gccgacatca ggacgtggtg ggcggtttgg ctgctgctga gcatcgcctg ccttggcatg
     1021 cagacgacgg tctggtccaa ggcagtcaat tcctgtttcg acaaggggcg cggggtcgct
     1081 ctggcgctta tggccagcgg caccggcata tcgaccatct ttctgccgac tctgtgcaat
     1141 ttcctgatcg gtaattacgg gtggcgcatc gccttcatcg cggtcggcgc gatctatgcg
     1201 gccgtgattt tgccgctgct gtggcgctgg ttctacgaag ccaccgacga ccggccgggg
     1261 acgcgagcgg caggcccgag ccgggccgag cggcaggccg gattgcccgg gtgggcggcg
     1321 cgggagggcc tgcgcaagcg gcagacctac cagatagcga tcgcgggcat gctggccgga
     1381 acggtgtgca ccggctattc cgtgcacatc gtctcgatgc tgaccgacaa gggcatggca
     1441 cgcgacgcgg ccgctgccat gctcagcgtg ctgggcctgc tggcggtaat ctcgcgcctg
     1501 gtgatcggcc gcatattcga ccgcgtgccg agccccttgg tagggatcat cagcgtcagc
     1561 ctgccggtga tccccggcct tctgctgttg ctgctgccgc ccacgccact ggtcggcctg
     1621 ctggctgcag cctcggtcgg cctcgccatc ggcggcgagt acgacaccgt gctctacatg
     1681 agcagccggt ttttcgggct gaaggcgttc ggcaccttgt tcggagtcgt ggcaacgatc
     1741 cttacggcgg gcttcgccat ggggccactg atcgccgggc gcatcttcga tctgaccggc
     1801 gactatcaac tctactttat cgggaccgtc ccgctgaccc tgatcgccgt gctgttgatc
     1861 gcgaccttgg ggccctatcc agatcatgcc cccgccgacg gcaggacggc ccacccggct
     1921 tagcttgccg ggtgagccgc cactgttcgg cagtaggcct attcggccgc catccgttcg
     1981 gccgattgct gttcgcccag caccatttcc cgcagttcgc ggtggaggtt ccacaccacc
     2041 ttttccgcaa gcggattggg cagggccccg gggaagccgg cggatttcat gccgcgttgg
     2101 acttcatcga cgttctcgta atcctggatg aagatccgcg gatagttgcc gtcgcgccag
     2161 tcttcgtaga actcatgcgt gaccttgggc gccttgccgg gggcgtagcg ttcgagcggc
     2221 atgatgtcga acaggcactt gtcggggtca tccccatctg gccgggcgcg gtagacgagc
     2281 aggccgtttt gcgtgggcag catggccatg ttggggaaca tgttccagtc gatcccgagc
     2341 ttgttgaaat cctcgagcga caggtgcgac aggtcgaccc cggcctcggt cgcgatgtcc
     2401 cgctccagcg tgagcagcgc caggcccatc tcgtccgacg tggcgtcggc ctcgacgcgt
     2461 tcgggaaggc gccggcccgc ttccaccaga tggtcggtga acatgcactg gagatcggcg
     2521 ttgagcacct gcatgtagag ggcgatgcgc gcgcgccgat cggtcacccc ttcgaacaga
     2581 tcggagttga acgggggcag gtactcggca ttgtggctgc gatcgccgag gttgccgacg
     2641 ttgtacatcg agtacatcga gtggcggcca aaggtctggc agatggagtg ggcgccgccg
     2701 tcgcagttgt tgacctggcg gtgggtggtc tgcgcgtgat agccttcgag gaatgcctcc
     2761 agcgcgactt tccagttgca gttgag
//
LOCUS       DATNBV010000538         2037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2209552,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000538 DATNBV010000000
VERSION     DATNBV010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2209552"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VN627_12785"
     CDS             <1..1032
                     /locus_tag="VN627_12785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXD04172.1"
                     /translation="DASELRGDGARIEWVASPRLPLDRRYRYFDDEAALHALLDALAP
                     DVVEASSPWGSASMIGRWRGPALRALVMHADPLSAYAYRWFGPLLSRETIDKRFERFW
                     EHLREMGEAFDLVVCASRELQERLAAGGVANTALHPMGIEAGLFGPERRSEAVRRDLL
                     ALCELPEDAHLLISVGRLAPEKRLPMVVDAVEAASHELPLGLVILGAGSQKRRILGQI
                     AGNPHIRLLSPERNRAKFATLLASADALIHGCEAETFCMAAAEARASGIPVIVPDRGG
                     AADHAGGGAGVRYAAGNAQAAARAIVDLFAGQVALSAEPVPVVTTADHFARLFAEYER
                     IAGAARQAA"
     gene            1277..>2037
                     /locus_tag="VN627_12790"
     CDS             1277..>2037
                     /locus_tag="VN627_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXD04173.1"
                     /translation="MLAGCGNGADKTKRPVPIVGYVVAEPTAVPLETSLGGRTVAFAT
                     SEVRPQVSGVIRSRLFEEGGVVRQGQPLFQIDPSLYRAAFNQASANLEAARANAEAAS
                     VKAERYRPLAEMEAISRQQYTDALAQSRQARASVAQNGAAVETARINLRYTTVPAPIS
                     GRIGRSLFTTGALVNASQADPLAVIQQVDPIYVDMQQSSAELLTLKRALASGGVSAGS
                     TSVRLKLEDGSDYPFAGTVQFSEMMVNEATGTITLR"
BASE COUNT          305 a          668 c          726 g          338 t
ORIGIN      
        1 gacgcgagcg agctgcgcgg cgacggagcg cggatcgaat gggtcgcctc gccgcggctg
       61 ccgctcgacc gccgctatcg ctatttcgac gatgaagcag cgttgcacgc gctgctcgat
      121 gcactcgcgc ccgatgtcgt cgaggcctct tcgccctggg gcagcgcatc gatgatcggg
      181 cgctggcgcg gtccggcgct gcgcgcgctg gtgatgcatg ccgatccgct ctcggcctat
      241 gcatatcgct ggttcgggcc gctgctcagc cgtgagacga tcgacaagcg cttcgagcgg
      301 ttctgggagc atctgcgcga aatgggcgag gcgtttgatc ttgtggtctg cgccagccgc
      361 gagttgcagg agcgcctggc ggctggcggg gttgccaaca cggcgctgca cccgatgggg
      421 atcgaagccg gcctgttcgg gcccgagcgg cgcagcgagg cggtgcggcg tgacctcctg
      481 gcgctgtgcg agctgcccga ggacgcgcac ctgttgatca gcgtcggtcg gctggcgccg
      541 gaaaagcgcc tgccgatggt ggtcgatgcg gttgaagccg ccagccatga gctgccgctc
      601 ggcctggtga tcctcggcgc gggaagccag aagcggcgga tcctggggca gattgccggc
      661 aacccgcata tccggctgct ctcgcccgag cgcaaccgcg ccaagttcgc caccctgctg
      721 gccagcgccg atgccctgat ccacggctgc gaggcggaga ccttctgcat ggcggcggcc
      781 gaagctcggg cgagcggcat tcccgtgatc gtgcccgatc gtgggggcgc ggccgatcat
      841 gccggcggcg gggccggggt gcgctatgcc gccggcaatg cgcaggccgc ggcccgggcc
      901 attgtcgatc tgttcgccgg ccaggttgcg ctgagcgccg agcccgtgcc ggtcgtcacc
      961 accgccgatc atttcgcccg gctgtttgcc gaatacgagc gcatcgccgg cgcggcgcga
     1021 caggcggcct gagcgtccgg catctgccca ccgggccgat ggtgcgccaa ttggttgcag
     1081 ggcaaattgc gtgtcgcaaa atgtcgcact gcaagaacaa ggtgtttcca atcgccctta
     1141 acaacgccaa ggaagacggc gcagcgcaaa atctggcgga ggcagtggtt tgataccgtc
     1201 gaagtggata ccggcggcgg atcgcgggag cggccgcagg tgggcaggag cgctggccgt
     1261 tgccacggcg ctcgccctgc tggccggttg cggcaacggg gctgacaaga ccaagcggcc
     1321 cgtgcccatt gtcggctatg ttgttgccga accgacagcg gttccgctgg aaacgtcgct
     1381 cggcggccga accgtggcct tcgctacctc tgaagtccgc ccgcaggtga gcggggtgat
     1441 ccggtcgcgg ctgttcgagg aaggcggtgt ggtgcgtcag ggccagccgc tgttccagat
     1501 cgatccgagc ctctatcgtg ccgcgttcaa ccaggcctcg gccaatctcg aggccgcccg
     1561 ggccaatgcc gaggctgctt cggtcaaggc ggagcggtat cgtccgctgg ccgagatgga
     1621 ggccatttct cgccagcaat ataccgacgc cctggcccag tcgcgccagg cccgggcttc
     1681 cgtcgcgcag aacggcgcgg cagtggagac ggcgcggatc aaccttcgtt ataccactgt
     1741 gcccgctccg atttcgggcc gcatcggccg ttcgctgttc accaccggcg cgctggtcaa
     1801 tgccagccag gccgatccgt tggcggtgat ccagcaggtc gatccgatct atgtcgacat
     1861 gcagcagtcc agcgccgaac tgcttacgct caagcgcgcg ctggcgagcg gcggcgtgtc
     1921 ggcgggcagc acttccgtgc ggctcaagct ggaggacggc agcgactacc ccttcgccgg
     1981 cacggtccag ttctccgaaa tgatggtgaa cgaggccacc gggacgatca ccctgcg
//
LOCUS       DATNBV010000539         3050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4776469,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000539 DATNBV010000000
VERSION     DATNBV010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4776469"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..997)
                     /gene="accD"
                     /locus_tag="VN627_12795"
     CDS             complement(149..997)
                     /gene="accD"
                     /locus_tag="VN627_12795"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HXD04174.1"
                     /translation="MSWLTRVRNALPFTPKRDTPDNLWIKCPSCSEMLFTKDYEANLS
                     VCPLCDHHGRVKTDERFAQLLDAGFAVLPTPKVKEDPLKFRDSKKYPDRLKAARAANP
                     HPDALTNASGAIDGHPVVLGVQDFAFLGGSMGMAVGAAFVAGAEAAIKAKVPYVICTA
                     AGGARMQEGILSLMQMPKTTVAVRRLARAGLPYVVVLTDPTTGGVTASYAMLGDIHIA
                     EPGSLIGFAGQRVIQDTIREKLPEGFQRAEYLKDHGMVDMVVHRRDLKATLAQVLRYL
                     QPAKAA"
     gene            complement(994..1794)
                     /gene="trpA"
                     /locus_tag="VN627_12800"
     CDS             complement(994..1794)
                     /gene="trpA"
                     /locus_tag="VN627_12800"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HXD04175.1"
                     /translation="MSRLADAFGKGRPALVTFVTGGDPTPDASAAILDALVDGGADVI
                     ELGMPFTDPMADGPAIQLANLRSLGAGTKTADIFAIAAAFRARHPEVPLVLMGYANPM
                     ITRGSAWFAGECVKAGVDGVICVDIPPEEDAELGPALRGAGVSLIRLATPTTDAARLP
                     AVLEGSSGFLYYVSVAGITGLQQAAQASIEEAVARLKASATIPVAVGFGVRTPEQAAA
                     IAKVADGVVVGSALVDLVAKHGANAAGPVRELTAALSQAVHQSRKEPA"
     gene            complement(1791..3026)
                     /gene="trpB"
                     /locus_tag="VN627_12805"
     CDS             complement(1791..3026)
                     /gene="trpB"
                     /locus_tag="VN627_12805"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HXD04176.1"
                     /translation="MTSPAPNSFRNQPDERGHFGQFGGRYVAETLMPLILDLEREYRA
                     AKADPAFAAEFDDLLEHYVGRPSPLYYAPRLTEALRTKADKGMGAQIWFKRDELNHTG
                     AHKINNCIGQILLAMRMGKTRIIAETGAGQHGVATATVCARFGLPCVVYMGATDVARQ
                     APNVFRMKLLGAEVVPVTAGAATLKDAMNEALRDWVANVHDTFYIIGTAAGPHPYPEL
                     VRDFQSVIGKEARAQMLSRTGRLPDLLVAAIGGGSNAIGLFHPFLDDPSVKMLGVEAA
                     GHGLDKEHAASLAGGRPGVLHGNKTYLLQDEDGQIVEGHSISAGLDYPGIGPEHAWLK
                     EIGRVEYTSATDKEALDAFQLLCRTEGIIPALEPSHAIAALARIAPTLSEDTIILANL
                     CGRGDKDIFSVAEHLGVSL"
BASE COUNT          476 a         1116 c          982 g          476 t
ORIGIN      
        1 cacgcataaa tcctccccac gagtggggag ggggaccgcc gggacggcgg tggagggggt
       61 tctcccccaa acgctgcgcc cgccggcgcg cccccaccac cacgcttcgc gcggtcaacc
      121 tccccacgag tggggaggaa cggctggatc acgccgcctt cgccggctgc agatagcgca
      181 gcacctgcgc cagcgtcgcc ttcaggtcgc gccggtggac gaccatgtcg accatgccgt
      241 ggtccttgag atactcggcc cgctggaacc cttcgggcag cttctcgcgg atcgtgtcct
      301 ggataacgcg ctggccggca aacccgatca ggctgcccgg ctcggcgatg tggatgtcgc
      361 cgagcatggc atagctcgcg gtcaccccgc ccgtggtcgg gtcggtcagc accacgacat
      421 agggcagccc ggcccgcgcc agccgccgca cggcgaccgt cgtcttgggc atctgcatca
      481 ggctgaggat gccctcctgc atccgcgccc cgccggcggc cgtgcagatc acatagggca
      541 ccttggcctt gatcgccgct tccgcgccgg ccacgaaggc cgcgcccacg gccatgccca
      601 tcgagccgcc aaggaaggca aagtcctgca cgcccagcac caccgggtga ccatcgatcg
      661 ccccgctggc attggtcagc gcatcgggat gcggattggc cgcgcgggcg gccttcaggc
      721 ggtcgggata cttcttggaa tcccggaact tgagcgggtc ttccttcacc ttgggcgtcg
      781 gcaacaccgc aaacccggcg tcgagcaact gggcaaatcg ttcgtccgtc ttcacccggc
      841 cgtggtgatc gcacagcggg cacaccgaga ggttcgcctc gtagtccttg gtgaacagca
      901 tctcgctgca cgaaggacac ttgatccaca ggttgtcggg cgtgtcgcgc ttgggggtaa
      961 acggaagcgc attgcggacg cgggtaagcc agctcatgcc ggttccttcc tcgattgatg
     1021 caccgcttgc gagagcgcag cggtcagctc gcgcaccggt ccggcggcat tggcgccatg
     1081 cttggccacc aggtcgacca gcgccgagcc gacgaccacg ccgtccgcca ccttggcgat
     1141 cgccgccgcc tgctcgggcg tacgcacgcc gaagcccacg gccacgggga tcgtcgccga
     1201 agccttgagc cgcgccacgg cttcctcgat gctcgcctgc gccgcctgct gcagcccggt
     1261 gatcccggcc accgaaacgt agtagagaaa cccggaagag ccctccagca ccgccggcag
     1321 ccgcgccgcg tcggtggtcg gcgtcgccag ccggatcagg ctcacgcccg ccccgcgcag
     1381 ggccgggcct agctcggcat cctcctcggg cggaatgtcg acgcagatca ccccgtccac
     1441 gccggccttc acgcattcgc cggcaaacca cgcgctcccg cgcgtgatca tcgggttggc
     1501 atagcccatc agcaccagcg gcacctcggg gtggcgcgcg cggaaggcgg cggcgatggc
     1561 gaagatgtcc gccgtcttcg tccccgcgcc aaggctgcgc aggttcgcca gctggatcgc
     1621 cgggccgtcc gccatcggat cggtaaacgg catgcccagc tcgatcacgt cggccccgcc
     1681 atccaccagc gcatcaagga tcgcggcgct cgcatccggc gtcggatcgc ccccggtcac
     1741 gaaggtgacg agggcagggc gccccttgcc aaaggcgtca gccagccggc tcacagcgaa
     1801 acccccaggt gctcggccac cgagaagata tccttgtccc cccggccgca caggttggcg
     1861 aggatgatcg tgtcctcgct cagcgtcggc gcgatcctgg ccagcgccgc aatggcgtgg
     1921 ctcggctcca gcgcggggat gatcccctcg gtccggcaca gcagctggaa cgcgtccagg
     1981 gcctccttgt cggtcgccga ggtatattcc acgcgcccga tctccttgag ccaggcatgc
     2041 tccggcccga tacccggata gtcgagcccg gccgagatcg aatggccctc cacgatctgc
     2101 ccgtcctcgt cctgcagcag ataggtcttg ttgccgtgaa gcacgcccgg ccgcccgccg
     2161 gccaatgaag ccgcatgctc cttgtcgagc ccatgcccgg ccgcttccac gcccagcatt
     2221 ttcaccgagg gatcatccag gaacggatgg aacagcccga tcgcgttcga tccaccgccg
     2281 atcgccgcca ccagcagatc aggcagccgc ccggtccggc tgagcatctg cgcccgcgct
     2341 tccttgccga tgaccgactg gaaatcgcgc accagctcag gatagggatg cggccccgcc
     2401 gccgtgccga tgatgtagaa cgtgtcgtgc acgttggcga cccagtcgcg cagcgcctcg
     2461 ttcatggcgt ccttgagcgt cgccgccccg gcggtcacgg gcacgacttc cgcgcccagc
     2521 agcttcatcc gaaacacgtt gggtgcctgc cgcgccacgt cggtcgcccc catgtagacc
     2581 acgcaaggca ggccgaaccg cgcgcagacc gtcgccgtcg ccacgccgtg ctggcccgcg
     2641 ccggtctcgg caatgatccg ggtcttgccc atccgcatcg ccagcaggat ctggccgatg
     2701 cagttgttga tcttgtgcgc gccggtgtgg ttcagttcgt cgcgcttgaa ccagatctgc
     2761 gcgcccatgc ccttgtccgc cttggtccgc agcgcctcgg tcagccgcgg cgcgtaatag
     2821 agcgggctcg gccggccgac atagtgctcg agcaggtcgt cgaactcggc ggcgaaggcc
     2881 ggatcggcct tggccgcgcg atattcgcgc tcgagatcga ggatcagcgg catcagcgtt
     2941 tcggccacgt agcgaccgcc aaactggccg aagtggccgc gctcgtcagg ctggttccgg
     3001 aagctgttgg gggcagggga agtcatgccg ctgcgcttgg cagagccctc
//
LOCUS       DATNBV010000540         4534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1876410,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000540 DATNBV010000000
VERSION     DATNBV010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876410"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /gene="guaA"
                     /locus_tag="VN627_12810"
     CDS             <1..1314
                     /gene="guaA"
                     /locus_tag="VN627_12810"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HXD04177.1"
                     /translation="GICYGQQALMQQLGGEVLLGDAGEFGRAFIDIEDRCVLFDGLWA
                     EGEKHQVWMSHGDKVTRLAPGFRAVAASPGAPFAVIANDEKRIYAMQFHPEVVHTPDG
                     GKLLKNFVRHVCGLAGDWTMAEFRKTKIAEIREQVGSGKVICGLSGGVDSAVAAVLIH
                     EAIGDQLTCVFVDHGLMRLGEAEQVVGLFKGHYNIPLIHKDVSALFLGGLAGVTDPEA
                     KRKFIGKTFIDVFEEEARGIGGAEFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGL
                     PERMNMKLVEPLRELFKDEVRELGRELGLPEIFVGRHPFPGPGLAIRIPGEVTRERCD
                     VLRKADQIYLEEIRNAGLYDAIWQAFAVLLPVRTVGVMGDGRTYDNVCALRAVTSTDG
                     MTADIYPFDAAFLSRVATRIINEVKGINRVVYDYTSKPPGTIEWE"
     gene            complement(1347..1715)
                     /locus_tag="VN627_12815"
     CDS             complement(1347..1715)
                     /locus_tag="VN627_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04178.1"
                     /translation="MSAVSASDCALPRDFASLEPWVAAGWAGQTAHDRSLLRVDRSPE
                     EVAAFHAAVAPLLDPALAYLDLFPVDRLPPAEERLMRLMLTFAHVALAVEMQGPDEVL
                     QRVATRHMRITRAVADRPPA"
     gene            complement(1712..2953)
                     /locus_tag="VN627_12820"
     CDS             complement(1712..2953)
                     /locus_tag="VN627_12820"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXD04179.1"
                     /translation="MDTIACTAGSELPGTPIDPAEYGPRTVATGPYYRRDWFEWEVEA
                     IFRREWLQVAHVSEVQNPGDFVVREVDCCKVSALIVKGKDGVIRAFHNVCTHRGTLLV
                     SDRPAGNAPVFTCRYHGWSFERDGSLRAAPDFDTFGLAKADCALKPIALDVCGGLIYI
                     NLDPQPRQSLAEFLGPAWDLLPYHAAATATGFSEYVYEIEGNWKLVYDNFQETYHLRF
                     IHPRSIGTSTSPAGNFFGYADTFATFGPHRTMLLPVNPEFEPAPTAARGFGKVIQSAM
                     ERGTELGPWNRYYFCMFPDNYLLLSQANPFVHSIMPISETRTRGTVRLYWNGEDASAS
                     ERFAREFAMAATRDIHSEDVSVIEACQKGILSRAIDRVHFQQHELFCHYLYDQARLRV
                     QDWLSQQGMADAADAALEFGR"
     gene            complement(3070..>4534)
                     /locus_tag="VN627_12825"
     CDS             complement(3070..>4534)
                     /locus_tag="VN627_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXD04180.1"
                     /translation="VGYLFAIGNPRADWLLRTEPEPGLNGRSLAYPRGRVLGGSSAIN
                     AMIAMRGQAADYDHWRDLGLPGWGWDDVLPAFRGIEDHFLGESAHHGAGGGWRVEPPR
                     VAWPVLDAVARAATQLGVPPTADFNTGNNEGVGYFHVNQKRGRRWSAARGFLAPIRSR
                     QNLRVMTGVTAECVELRDGRATGVRLRAGNRLMTARARGEVVLCAGAIGSVQILHRSG
                     IGPGEWLAPLGIPVALDRPGVGRNLQDHLQQRAIYRVHGIGTLNEAYHSLPRRALMGL
                     DYLLRRRGPLTMAPSQLGLFTRSRPEVDRPDLQFHVQPLSLDKFGDPLHREPAITVAA
                     CNLRPTSRGTVRLASPDPAAPPRISPAYLSTAEDRRVAADAIRVTRRLMAQPALAPYC
                     PEEVLPGPAVGDDDVALAGAAGDIGTTIFHPVGTAKMGAADDPLAVLDERLRLRGVRD
                     LRVIDASVMPTITSGNTNTPTAMIAYMGTRYLLADCG"
BASE COUNT          748 a         1464 c         1643 g          679 t
ORIGIN      
        1 ggcatctgct atggccagca ggcgctgatg cagcagctgg gcggcgaggt cctgctcggc
       61 gatgccggcg aattcggccg cgccttcatc gacatcgaag accgctgcgt cctgttcgac
      121 gggctctggg ccgagggcga gaagcaccag gtgtggatga gccacggcga caaggtcacc
      181 cggctcgccc ccggcttccg cgccgtcgcc gccagcccgg gcgcgccctt cgcggtgatc
      241 gccaatgacg agaagcgcat ctacgcgatg caattccacc ccgaggtggt ccacacgccc
      301 gacggcggca agctgctcaa gaacttcgtc cgccacgtct gcggcctcgc cggcgactgg
      361 accatggccg agttccgcaa gaccaagatc gccgagatcc gcgaacaggt cggcagcggc
      421 aaggtcatct gcggcctctc gggcggcgtc gacagcgcgg tcgccgccgt gctcatccat
      481 gaggcgatcg gcgaccagct gacctgcgtc ttcgtcgacc atggcctgat gcggctgggc
      541 gaggccgaac aggtcgtcgg cctgttcaag ggccactaca atatcccgct gattcacaag
      601 gacgtctcgg ccctattcct cggcggactc gccggcgtca ccgatcccga ggccaagcgc
      661 aagttcatcg gcaagacctt catcgacgtg ttcgaggaag aagcgcgcgg gatcggcggg
      721 gccgaattcc tcgcccaggg cacgctctac ccggacgtga tcgagagcgt cagcttcacc
      781 ggcgggcctt cagtgacaat caagagccac cataacgtcg gcggactgcc cgagcggatg
      841 aacatgaagc tcgtcgagcc cctgcgcgag ctgttcaagg atgaggtgcg tgagctgggc
      901 cgcgagctgg gcctgccgga gatcttcgtc ggccgtcatc ccttccccgg gccgggtctc
      961 gccatccgca tccccggtga ggtgacgcgc gagcgctgcg acgtgctgcg caaggctgac
     1021 cagatctacc ttgaggagat ccggaatgcc gggctctacg acgcgatctg gcaggccttc
     1081 gccgtgctgc tgccggtgcg cacggtgggc gtgatgggcg acgggcggac ctacgacaac
     1141 gtctgtgcgc tgcgtgccgt gaccagcacc gacggcatga ccgccgatat ctacccgttc
     1201 gacgccgcct tcctcagccg cgtcgccacg cgcatcatca acgaggtcaa gggcatcaac
     1261 cgggtggtct acgactatac ctcgaagccg ccgggcacga tcgagtggga gtgagcgatc
     1321 gcgccgaccg gcgccgaagg tgagcctcag gccggcgggc ggtcggccac ggcgcgggta
     1381 atgcgcatgt ggcgggtggc cacgcgctgc aggacttcgt cggggccctg catctcgacg
     1441 gccagggcca cgtgggcgaa ggtcagcatc agccgcatca ggcgttcctc ggccgggggc
     1501 aggcgatcga ccggaaagag gtcgagatag gccagggccg ggtcaagcag cggcgccacg
     1561 gcggcgtgga atgcggcgac ctcttccggc gaacggtcca cccgcagcag cgagcggtca
     1621 tgcgcggtct gaccggccca gccggcggcg acccacggct caaggctggc gaaatcgcga
     1681 ggcagcgcgc agtcgctggc actcacggcg ctcaccggcc aaactccagg gctgcgtcgg
     1741 ccgcatcggc catgccctgc tgagagagcc agtcctgcac gcgcaggcgc gcctggtcat
     1801 aaagatagtg gcagaacaac tcgtgctgct ggaaatgcac gcggtcgatc gcccggctta
     1861 gaatgccctt ctggcaggcc tcgatcaccg atacgtcctc cgaatggata tcgcgggtgg
     1921 cggccatggc gaattcgcgg gcaaagcgct cgctcgcgct ggcgtcctcg ccgttccaat
     1981 agagccgcac ggtcccgcgg gtgcgggttt ccgatatcgg catgatcgaa tggacgaagg
     2041 gattggcctg gctcagcagc aggtaattgt cggggaacat gcagaaatag taccggttcc
     2101 agggccccag ttcggtgccc cgttccatgg cgctctggat caccttgccg aagccccgcg
     2161 ccgccgtggg ggccggctcg aactcggggt tcaccggcag cagcattgtg cggtgggggc
     2221 cgaaggtggc gaaagtgtcg gcatagccga agaaattgcc cgccggcgag gtactggtgc
     2281 cgatgctgcg tggatggatg aagcgcaggt gataggtctc ctggaaattg tcgtagacga
     2341 gcttccagtt accttcgatc tcgtagacgt attccgaaaa acccgtggcc gtggcggcgg
     2401 cgtgataggg caggaggtcc caggcgggcc cgaggaattc ggccaggctc tggcggggct
     2461 ggggatcgag gttgatgtag atcagcccgc cgcagacatc gagcgcgatg ggcttgagcg
     2521 cacagtcggc cttggcgagg ccgaaggtgt cgaaatcggg ggcggcgcgc aggctgccgt
     2581 cgcgctcgaa gctccagccg tgatagcggc aggtgaaaac cggcgcgttg ccggccgggc
     2641 gatcgctcac cagcagggtg ccgcgatggg tgcagacatt gtggaaggcg cggatcaccc
     2701 cgtccttgcc cttgacgatc agggcggaaa ccttgcagca gtcgacctcg cggacgacaa
     2761 aatcgccggg gttctgcacc tcgctgacgt gcgcgacctg gagccattcg cgccggaaga
     2821 tcgcctcgac ctcccattcg aaccagtcgc gccggtaata gggccccgtc gctacggtgc
     2881 gcgggccgta ttcggccgga tcgatgggcg taccgggcag ttcgctgccg gccgtgcagg
     2941 caatggtatc catggacttc tctccggtcc gcgtccccgc ctgaccgcgg ctggcgacgc
     3001 tgccggagtc agggcttaca acgaggtaag cacggcgggg cgggcaaggc aacgcccgca
     3061 accctttgtt tatccgcaat cggcaagcag gtacctagtt cccatatatg cgatcattgc
     3121 cgtcggcgtg ttcgtattgc cggaggtgat cgtcggcatg accgaggcat cgatcacgcg
     3181 caggtcccgc acgccgcgca ggcgcaggcg ttcgtcgagg acggcgagcg ggtcgtcggc
     3241 cgcgcccatc ttcgccgtgc cgaccgggtg gaagatcgtc gtgccgatat cgccggcggc
     3301 accggcgagg gcgacatcgt cgtcgcccac tgccgggccg ggcaggactt cctcggggca
     3361 gtagggcgcc agggctggct gcgccatgag gcggcgggtg acgcggatcg cgtcggcggc
     3421 gacgcggcgg tcctccgcgg tggagagata cgcgggcgag atgcgcgggg gcgcagcggg
     3481 atcgggggag gcgaggcgga cggtgccgcg cgaggtgggc ctgaggttgc aggcggcgac
     3541 ggtgatcgcc ggctcgcggt gcagcggatc gccgaacttg tcgaggctga gcggctggac
     3601 gtggaactgg aggtcggggc ggtccacttc gggccgggag cgggtgaaca ggcccagctg
     3661 cgagggggcc atggtcagcg ggccgcgccg gcgcagcagg tagtcgagcc ccatcagcgc
     3721 gcggcggggc agcgaatgat aggcctcgtt gagcgtgccg atgccgtgga cccggtagat
     3781 cgcgcgctgc tggagatggt cctgcaggtt gcggccgacc ccgggacgat cgagcgcgac
     3841 ggggatgccg agcggggcca gccattcgcc cgggccaatg ccggagcggt gaaggatctg
     3901 gaccgagccg atcgcgccgg cacagagcac gacctcgccc cttgcccggg cggtcatcag
     3961 gcgattgccc gcgcggaggc gcacccccgt ggcacggcca tcgcgcagtt ccacgcattc
     4021 ggcggtgacc ccggtcatca cgcgcaggtt ctggcgggag cggatggggg cgaggaagcc
     4081 gcgcgcagcc gaccagcgcc gcccgcgctt ctggttgacg tggaaatagc ccacgccctc
     4141 gttgttgccg gtgttgaaat cggcagtcgg cggaacgccc agctgggtcg cggcgcgggc
     4201 gacggcatcg agcactggcc atgcaacgcg cggcggctcg acccgccagc cgccgccggc
     4261 gccgtgatgc gcgctttccc cgaggaagtg gtcctcgatg ccgcgaaagg cggggagcac
     4321 atcgtcccac ccccagccgg gcaggcccag atcgcgccag tgatcatagt ccgccgcctg
     4381 cccgcgcatg gcaatcatcg cgttgatcgc cgaggagccg cccagcacgc gcccgcgcgg
     4441 ataggcgagc gagcggccat tgagacccgg ctcgggctcg gtgcgcagca gccagtcggc
     4501 ccggggattg ccgatggcga agaggtatcc gacg
//
LOCUS       DATNBV010000541         6649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1879214,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000541 DATNBV010000000
VERSION     DATNBV010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879214"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /gene="lptD"
                     /locus_tag="VN627_12830"
     CDS             complement(<1..1610)
                     /gene="lptD"
                     /locus_tag="VN627_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HXD04181.1"
                     /translation="MLGLLLAAPALAQESAPAQLPDAPVVTAPAGNPPISFEADQVTY
                     GWNDEVVTATGSVVLRRPSGEATQSVRADKIVWNRKTGVVEASGRVRLVDDDGNQVFT
                     DHVELTDELKAGAMENLLIALRDGGRLAAVSGKRDLQGRIVLERAAYSGCPVEDGNGC
                     PHNPSWQVNARRVVYDPARREIHFTGARLRLLGITLMPLPGLTIASDGRPINGLLIPD
                     LQVTANNGIQVSDTWYQRLGPNHDLAVTGYLFTQALPMVSARYRALLDDGAFQITGYA
                     TRSRRTSVTGSAVSNSSVTDFRGYLAANGRFQFSPEWSLSSSIRVASDRTFLRRYDIN
                     RDDRLRSTVELERIGQNSYFSLAGWATQTMRVGDRQGLVPVALPVVDWRQRLSDPVLG
                     GKVELQLNTLAITRTAGQDTQRAFVKAQWDLTKVLPGGQLVSLTALARGDVYHSDANA
                     LTTTALYRGDPGWQGRGIAIGALDVKWPLVGSAFGGTQVFTPRFQLVASPPVRNLAVP
                     NEDSRAIDLEDSNLFALNRFPGYDRVEDG"
     gene            1819..3285
                     /locus_tag="VN627_12835"
     CDS             1819..3285
                     /locus_tag="VN627_12835"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HXD04182.1"
                     /translation="MKITFAASIPADAKLVAQVCNQDALPAGLEPVLAAAAGASRFTG
                     KVGQVFEGFVERGGQVVRVALAGAGAGAGPTGADRTGALEKAGAGLTAKYLTSGERVM
                     ALDLSGAGLSAQEACAVLLGVRLRGWRLDTYRTTLKPEQAPSLCQVAVVGAPDGTEAL
                     WAVESAVAAGVEFTRELVTEPANVIYPESFVARCQARFAGTGLELRVLDENEMTALGM
                     GALLGVAQGSARPPRLLAAIWRGDGEAPFTAFVGKGVTFDTGGISIKPAAGMEDMKWD
                     MGGAGAVAGTMLALAGRKARANVIGVVGLVENMPDGKAQRPGDVVTTLSGQTVEVINT
                     DAEGRLVLCDVLTWVQREYKPAAIVDLATLTGAIIISLGHEHAGLFTNDDELAAALDS
                     AGKASGDKLWRFPLGPAYDKLIDSPIADIKNVGPRYGGSITAAQFLQRFIDKGTPWAH
                     LDIAGTVWTDKPGATWDKGASGFGVRLLDRYVRDRIEG"
     gene            3326..3784
                     /locus_tag="VN627_12840"
     CDS             3326..3784
                     /locus_tag="VN627_12840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HXD04183.1"
                     /translation="MQVDFYQVSRDPAEAIVAMLAEKTLGAGQRLLVVAAPDERLEAI
                     GQALWSRSGRDGAPTFLANGRAGGGHESRQPVLLAGAVEPANGARFVALADGVWRDEA
                     ADPERFDRAFLVFDGATLDAARACWRMLGEREGLVRNFWKQDGGRWVKAA"
     gene            3894..3969
                     /locus_tag="VN627_12845"
     tRNA            3894..3969
                     /locus_tag="VN627_12845"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3927..3929,aa:Thr,seq:cgt)
     gene            4119..4730
                     /locus_tag="VN627_12850"
     CDS             4119..4730
                     /locus_tag="VN627_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HXD04184.1"
                     /translation="MIVRETGATSMDEEAKFVDHYIVLGVHPETNSRDIEAAYRHLAM
                     RYHPDHPGTADVDRFRQILAAYRALKSPEERARYNHEYAKATGFEFGASVDREEAFGE
                     ALSDAAAHAKILSVLYKRRRESTREPGLGHYMVQQELNCSDESFEFFVWYLKEKGFIA
                     VTEQGQLAITIVGVDHVISTSKADAKQRLRITQLDSFGDEPTS"
     gene            4840..5016
                     /locus_tag="VN627_12855"
     CDS             4840..5016
                     /locus_tag="VN627_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04185.1"
                     /translation="MGVDPLRLTIEQDIDDCTTELLALEKDPERSRSTEEILLLKQRL
                     FNLCAALAHLGDNG"
     gene            complement(5047..5424)
                     /locus_tag="VN627_12860"
     CDS             complement(5047..5424)
                     /locus_tag="VN627_12860"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HXD04186.1"
                     /translation="MPSPSGDDPQAEALKRMELVANLLDSAFLVPGTNQRIGIDAIIG
                     LVPGIGDVITTLLSSYVLWEAKRLGVSRLAMTRMLGNLAVHATLGSVPLIGDAFDAWF
                     RVNQRNLKIVRSHIARRPQATRS"
     gene            5765..>6649
                     /locus_tag="VN627_12865"
     CDS             5765..>6649
                     /locus_tag="VN627_12865"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXD04187.1"
                     /translation="MPNFSCLAVPLSTPPMEARLVPELPTGAEWQFEPKWDGFRCLAF
                     RDGGAVELMSKSGKPLGRYFPEVVATVLSMPDARLVLDGELVLPRGETLSFADLQLRL
                     HPSASRIARLARETPAQLMVFDCLQRGSEMLVTQPLAKRRAILEDLARQGLPPQMRLS
                     PATDDPATARAWLARSEGALDGIVAKQRDQPYRPGERAMVKYKVRRTADCVIGGYRTD
                     KAGTALASLLLGLFDDEERLHHVGFVAGLDGGQRAALLERLRPLAAQSSFDGNAPGGK
                     SRWSGERSTAWCPVRPELV"
BASE COUNT         1099 a         2079 c         2400 g         1071 t
ORIGIN      
        1 ccgtcctcca cccggtcgta gccggggaag cggttgaggg cgaagaggtt ggaatcctca
       61 aggtcgatcg cccgcgaatc ctcgttgggg acggccagat tgcgcaccgg cgggctggcg
      121 acgagctgga agcgcggggt aaacacctgg gtgccgccga aggcgctgcc gaccagcggc
      181 cacttcacat cgagcgcgcc gatggcgatg ccccggccct gccagccggg gtcgccgcga
      241 tagagcgcgg tcgtggtcag cgcgttcgcg tcggagtgat agacatcgcc gcgggcgagc
      301 gcggtgaggc tgacgagctg gccgccgggg agcactttcg tgaggtccca ctgggcctta
      361 acgaaggcgc gctgggtatc ctgcccggcg gtgcgggtga tggcgagggt gttgagctgc
      421 agctcgacct tgccgccgag gaccggatcg gacagccgct ggcgccagtc caccaccggc
      481 agggcgacgg ggacgaggcc ctgccggtcc cccacccgca tggtctgcgt ggcccatccg
      541 gccagcgaga aatagctgtt ctggccgatc cgctcgagct ccacggtgga gcgcaggcga
      601 tcgtcgcgat tgatgtcata gcggcgcagg aaagtacggt cgctcgcgac gcggatcgag
      661 ctggacaggc tccattccgg cgagaactgg aacctgccgt tggcggcgag atagccgcgg
      721 aagtcagtca ccgaggaatt ggacaccgcg ctgccggtga ccgaggtgcg gcggctgcgg
      781 gtggcatagc cggtgatctg gaaggcgccg tcatccagca gggcgcggta gcgggccgag
      841 accatcggga gggcctgggt gaacaggtag ccggtcaccg ccagatcatg attggggccg
      901 aggcgctggt accaggtatc gctgacctgg atgccgttgt tggcggtcac ctgcaggtcc
      961 gggatcagga ggccattgat cgggcggccg tcgctggcga tggtgaggcc gggcagcggc
     1021 atcagcgtga tgcccagcag gcgcaggcgc gcgccggtga aatggatctc gcgcctggcc
     1081 gggtcgtaga ccacgcggcg ggcattgacc tgccatgacg ggttgtgcgg gcagccattg
     1141 ccgtcttcca ccgggcagcc cgaataggcc gcgcgttcca gcacgatccg gccctggaga
     1201 tcgcgcttgc ccgagacggc ggcgagccgc ccgccgtcgc gcagggcgat cagcaggttt
     1261 tccatagcgc cggccttgag ctcgtcggtc agctcgacat ggtcggtgaa gacctggttg
     1321 ccgtcgtcat cgaccaggcg gacgcggccg ctggcctcga ccacgccggt cttgcggttc
     1381 cacacgatct tgtcggcccg gaccgactgc gtggcctctc ccgaaggacg gcgcagcacg
     1441 acgctgccgg tcgcggtgac cacctcatcg ttccagccgt aggtcacctg gtcggcttcg
     1501 aagctgatcg gcggattgcc agccggggcc gtgacgacag gcgcatcagg gagctgcgcc
     1561 ggcgctgatt cctgggccag ggccggggcc gccagcagga ggccgagcac cgctgcagag
     1621 cctgcgagca agcgggccga ggcccatttt ccgcgtggtc cggttccccg cggggcaggg
     1681 ctggtggcac gggcagggca ggggagcatg gcttgcctat cgcaccgccg ggccctaact
     1741 gcaatcgcgt aggcggcgga aaaacgtcgc ggtccgacac ccggggccaa ttgcctgccc
     1801 ttcgttgatg gagttcccat gaagatcacg ttcgccgcct cgattcccgc cgatgcgaag
     1861 ctggttgcgc aggtttgcaa ccaggacgcc ctgccggcgg gactggagcc ggtgctcgcc
     1921 gccgcggccg gtgcttcgcg cttcacgggc aaggtcggcc aggtgttcga gggcttcgtt
     1981 gagcgcggcg gccaggtggt gcgcgtggct ttggcgggag ccggcgccgg ggctggccct
     2041 acgggcgcgg accgcacggg cgcgctggaa aaggccgggg ccgggctgac ggcgaagtac
     2101 ctcacctccg gcgagagggt catggcgctg gacctgtccg gcgcgggcct ttcggcgcaa
     2161 gaggcctgcg cggtgctgct cggcgtgcgg ctgcgcggct ggcggctcga tacctatcgc
     2221 acgacgctca agcccgagca ggcgccttcg ctgtgccagg tcgcggtggt tggcgcgccg
     2281 gacgggaccg aggccctgtg ggcggtggaa agcgccgtgg ccgccggggt ggagttcacc
     2341 cgcgagctgg tgaccgagcc ggccaacgtg atctatccgg aaagcttcgt ggcccgctgc
     2401 caggcgcgct ttgccgggac cggccttgag ctgcgcgtgc tggacgagaa cgagatgacc
     2461 gcgctgggga tgggtgcgct gctgggcgtg gcgcagggtt cggcccgtcc gccccggctg
     2521 cttgcggcga tctggcgcgg ggacggggaa gcaccgttca ccgcatttgt gggcaagggc
     2581 gtgaccttcg atacgggcgg catttcgatc aagccggcgg ccggcatgga agacatgaag
     2641 tgggacatgg gcggggcagg cgccgtggcc gggaccatgc tggcgctggc cgggcgcaag
     2701 gccagggcca atgtgatcgg cgtggtcggg ctggtcgaga acatgcccga cggcaaggcc
     2761 cagcgccccg gtgacgtggt gaccacgctg tcgggccaga cggtcgaggt gatcaacacc
     2821 gacgcggaag gacggctggt gctgtgcgac gtgctgacct gggtccagcg tgaatacaag
     2881 ccggcggcca tcgtcgacct cgccacgctg accggcgcga tcatcatctc gctcggccac
     2941 gagcatgccg gcctgttcac caatgacgac gagctggccg ccgcgctcga ctccgcgggc
     3001 aaggcgagcg gcgacaagtt gtggcgcttc cccctgggcc cggcctacga caagctgatc
     3061 gacagcccga tcgccgacat caagaacgtc ggcccgcgct atggcggctc gatcaccgcg
     3121 gcgcagttcc tccagcggtt catcgacaag ggaactccct gggcccacct cgacatcgcc
     3181 gggacggtgt ggaccgacaa gccgggcgcg acctgggaca agggcgccag cggcttcggc
     3241 gtgcggctgc tcgaccgcta tgtgcgcgac cggatcgaag gctgaggtaa acggccgtct
     3301 ggcgacataa aggggggcgg gcgcggtgca ggtcgacttc taccaggtga gccgcgatcc
     3361 cgccgaggca atcgtggcca tgctggcgga aaagacgctg ggcgcgggcc agcgcctgct
     3421 ggtcgtcgcc gccccggacg agcggctgga ggccatcggc caggcgctgt ggagccggag
     3481 cggccgggac ggggcgccga ccttcctggc caacggccgc gcgggcggag gtcacgaatc
     3541 gcgccagccg gtgctgctgg ccggagcggt cgagccggcg aacggcgccc gcttcgtggc
     3601 gctggccgac ggggtatggc gcgacgaggc ggccgatccg gaaaggttcg accgggcctt
     3661 cctcgttttc gacggcgcca ccctggacgc ggcccgcgcc tgctggcgca tgctcggcga
     3721 gcgcgagggg cttgtgcgca acttctggaa gcaggacggc ggccgctggg tgaaggcagc
     3781 ctgagcgggg cgcctgatat cgggcgcgcg ccaaggctgc gtttaaggca ttgccttgcc
     3841 cgcaacggct ggctaaagcg ccgccctgcc tgcaggccat ccggccacgg catgccgctt
     3901 tagctcagtc ggtagagcac atcattcgta atgatggggt caggtgttcg aatcacctaa
     3961 gcggcaccat tttcccgcaa tttcagtgaa gctctggggc ggcaattggc ttcccgggtg
     4021 atgccctgcg ggtatggtgc gggctaattg ccaatagctt cgcttgtctg cgcactgaaa
     4081 atgtcagatg acgtacaggt acgactggac ggtcgaagat gatcgttcgc gaaactgggg
     4141 caacctcgat ggacgaagag gcgaaattcg tcgaccatta catagtactg ggcgttcacc
     4201 cggaaacgaa cagccgggac attgaagctg cctatcgcca cctggcaatg cgttatcatc
     4261 cagatcaccc cggcacggct gacgtggaca gatttcgcca aatcctggcg gcctatcgcg
     4321 cgttgaaatc gccggaggag cgagcgcggt acaatcacga atatgccaag gccaccggct
     4381 ttgaatttgg cgcatccgtc gatcgtgagg aggcattcgg cgaagcgctg tcggatgccg
     4441 ccgcccatgc aaagattctt tctgttctct acaagaggcg ccgcgaatct acccgggaac
     4501 ccggattggg ccactatatg gttcagcagg agctgaactg ctccgacgaa agtttcgagt
     4561 ttttcgtgtg gtacctgaag gagaagggct tcatcgcggt taccgagcag ggccagttgg
     4621 cgatcaccat cgttggcgtg gatcacgtta tttcaacgtc gaaggcggat gccaagcaga
     4681 gactgcggat cacgcaattg gactccttcg gggacgagcc gacgagttag acgggcgcag
     4741 tgattgccgt aaccgattga aattccgtct gagatccaca aggctgttta cctcgagagg
     4801 ccggcaagtt agatttcggc cggggcagaa gggggcccca tgggtgtcga tccacttcgc
     4861 ctgacgatcg aacaggatat cgacgactgc accaccgaac tgctggccct ggaaaaggac
     4921 ccggagcgat cccggtcaac cgaagaaatc ctgttgctca agcagcggct gttcaacctt
     4981 tgtgccgcgt tggctcacct gggagataat gggtgatctg gcgggcataa cggcgcggcc
     5041 gcgcgctcaa ctccgcgtag cctgcggacg gcgggcgatg tgagagcgca cgatcttgag
     5101 gttgcgctgg ttgacccgga accaggcatc gaaggcatcg ccgatcaagg gcaccgagcc
     5161 gagcgtggca tggaccgcca ggtttcccag catgcgcgtc atcgccaggc gcgagacgcc
     5221 gaggcgcttc gcttcccaca ggacgtagct tgacagcagc gtggtgatca cgtcgccgat
     5281 ccccggaacc agtccgatga tcgcatcgat gccgatccgc tggttcgtgc cggggacgag
     5341 gaaggcactg tcgagcaggt tggcgacaag ctccatgcgc ttcagtgcct cggcctgcgg
     5401 atcgtcgcct gacgggctgg gcatgcggga aaggcgtccg gtctggtaat tcgccacatt
     5461 ggtctccggc ctggccccac gggagggatg tggcgaccaa cggcgggcct tcaagcaggc
     5521 ggcgtgcgac ttgcggcgaa ccgagcggga tcggcgggcc cagggtcagg accctagctt
     5581 tccgcgctgc ccggcagatc gaagagattg cggtactggt gtcgaacccg gccccgccca
     5641 tggcgatcgg ctcgaacggg ccgggtgaca ccaaggcggt actcctcttc acactcgggc
     5701 ggcctcgacc agccgaccat ttggtgccgc cggaacgcac tgccaatgca atctttccac
     5761 cagcatgccg aacttctcgt gtctggccgt gccgctgtcg actccgccga tggaagcgcg
     5821 ccttgtcccg gaactgccga ccggcgcgga atggcagttc gagccgaagt gggacggctt
     5881 ccggtgcctg gcctttcgcg acggcggagc ggtggagctc atgtcgaaat cgggcaagcc
     5941 gctgggccgc tactttcccg aggtcgtggc aaccgtcctg agcatgcctg acgcgcggct
     6001 ggttctcgac ggggaactcg tgctgccgcg gggagaaacc ttgtcctttg ccgacctgca
     6061 attgcggctg cacccatcgg ccagcaggat cgcgcgcctg gcgcgggaaa cgccggccca
     6121 gctgatggtg ttcgattgcc tgcaacgcgg cagcgaaatg ctcgtgacgc agccgctcgc
     6181 gaaacgccgg gccattctgg aggacctggc gcgccaggga ctgccgccgc agatgagatt
     6241 atcgcccgcg acagatgatc ccgcgacagc ccgcgcgtgg ttggcgcgca gcgagggtgc
     6301 gctcgacggc attgtcgcca agcagcgtga ccagccctat cggcccggcg agcgggccat
     6361 ggtcaagtac aaggtgcgcc ggaccgcgga ctgcgtgatc ggcggttatc gcaccgacaa
     6421 ggccggaacc gcgcttgcct ctctgctgct cggcctgttc gatgacgagg agcggctcca
     6481 tcatgtgggg ttcgtcgccg gactggatgg cggccagcgt gccgccctgc tggaacgcct
     6541 gcgaccgctc gccgcgcaat cgtcattcga cgggaatgcc ccaggcggga agagccgctg
     6601 gagcggcgag cgcagcaccg cctggtgtcc ggtcaggccc gaactggtg
//
LOCUS       DATNBV010000542         6185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4140742,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000542 DATNBV010000000
VERSION     DATNBV010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4140742"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VN627_12870"
     CDS             <1..583
                     /locus_tag="VN627_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04188.1"
                     /translation="QSERLFQAYEIAKGLLRELKIFPGEGRERDILDIDEFDRSWPTM
                     KLDDLRYLVSAIISIGEKSGEGEPPFRESQFYGKWNTARRRILPHFNSGDSDAEAKKT
                     KINSPIGWKALMAKLSRLQRLRIFDRGRDRAIEYDDEGAVAVAELLLAHGADASVRNT
                     AGLTPLKSPRRAVSRRRPTCSIPVRGREDGRTT"
     gene            complement(872..3196)
                     /locus_tag="VN627_12875"
     CDS             complement(872..3196)
                     /locus_tag="VN627_12875"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXD04189.1"
                     /translation="MYISPKASRLRQREAQRARDNRAEIRKALSKGEISKRDLIRWGI
                     FTATGALALKNGLSPFARSAFADVPTGTPRSPLFGAARFTQRMPRLRLQTPIPLVRTA
                     ANEAVFPAALGYPNAKRLSYHNDFNDFVDPVTGQPVPAADNPFRNPVTNRGPMEGRPP
                     GEFFAHQRWEEFFPKVGYAMSWTQCAPGTKFHPNFPDQAPDSVWCYGTGNTHAGFMPP
                     FLIKGRYGEPILTRIYNNTPVDREDNGPNHFGRNETQLHFHNAHNGAESDGATGAHHF
                     PGTFYDYRWSTTLARRDRINVNGTDQRAAGPDNGSGIVKVPGDWREIQGTMWAHDHRF
                     FFTAENVYKGNLGMVNYYSGRDRGHETVTSGGNLRLPSGTRLGFGNTDFDVNLVVSDG
                     ATTDPDGQYFFDIFTTDGFLGDLPLVNFAYAPYFEVLPRKYRFRILNGSMSRYYQLAL
                     AYNGAAVPFKFIANDGNLVVSPITLTALDEQGIAERYDIVVDFSIFPVNSRIKLVNLL
                     KMRDDGRGPQGALSLAEALAGLLSDPVVGPMMEFRVVNSVPSVDQPGTTLTVANSCGP
                     NDRSQVPAVLTEQIPIVAPVRTRTVAFGRSGPGDSRDPVTGQCTPDCPEGMPFPWTIE
                     VNGGPAHSFNANRISELIPKPGEIEHWTLVNKGGGWDHPIHLHFEEGITMDRGADTIP
                     ATELLVRKDVWRLRDSGQVTFQVQFGEYGGSYVNHCHNTVHEDFALLMRIQLLSAAGP
                     QVAVTPTPNPTPDGVFFTTPEILPEGDPRGGATG"
     gene            complement(3275..3826)
                     /locus_tag="VN627_12880"
     CDS             complement(3275..3826)
                     /locus_tag="VN627_12880"
                     /inference="COORDINATES: protein motif:HMM:NF014813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HXD04190.1"
                     /translation="MAGPARPQVAAQVRPREARQARFARSFAQVVAVLMRDPNFRTMP
                     LADLEWLVLPAVMSGQFRVAEAPASLGPGKGPDKAADKAPDKTRTGGVLVPVAVALWA
                     RVSGEIDKALAGSLDQPVRLKPHQWASGEHVWLVAVAGDRRAVPKLIEQIARTDLKGQ
                     RIKMRVKGTGNTVVVKTLGEARD"
     gene            complement(4069..>6185)
                     /locus_tag="VN627_12885"
     CDS             complement(4069..>6185)
                     /locus_tag="VN627_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HXD04191.1"
                     /translation="VANVNDLPTAGPTISDTTPTEGRALTVDPATIVDPDGTATAVAG
                     GLFAFRWQQSADGITWVDAVGDLTDGTGQLFVPTQAQVGLQLRVLVTYTDDQGTVETV
                     ASAATQVVGDLIFGTNGPDTFIGTDGQDEIFGQGGADILNGLAGNDLLDGGAGNDRLD
                     GGLGADTMSGGLGNDIYFVDNAGDQVLENPGEGTDTVQTDLSSYTLTANVETLTFIGA
                     GGFTGTGNDLANTINGGFDSDTLLGGAGNDTLNGNVGADTLTGGAGDDTVNGGADDDR
                     LVAGVGDGNDAYNGGAGIDTYDLSATAAGAVVTATTSTSAETGTDTLAGVENVIGSQG
                     SDTITGNGGVNVIDGQGGNDTINAGGGNDTVSGGAGDDTLTGGTGNDILSGGLGNDSF
                     IFNFGDGADTVDGGAGTDTLSVTGTAANDTLDVIFDGVSLTGLELGTVTSVEAITADL
                     LGGTDRLSYAGSTADVTVNLAAGTASGFAAIAGIDNVTGGSGNDTLSGAGNAAVNNLA
                     GGAGNDTYLADNGDTITEAAGAGIDSVLTSSASFTLAANVDNLTFTGAGGFTGTGNGS
                     DNVIAGAGGTDVLAGGGGNDTLIGNGGVDSLDGGAGADTLIGGAGNDVMNGGTGNDSF
                     VFAAGFGNDTISGFDANPTGGQDLLDISALGITAANFAASVLITDLGNDTLVAIGGDT
                     ILLLGVNGVGANTITQQDFILH"
BASE COUNT          863 a         2094 c         2178 g         1050 t
ORIGIN      
        1 ccagagcgag cgcctgttcc aggcttacga gatcgccaag ggcctgttgc gcgagctgaa
       61 aatctttccc ggcgagggcc gcgagcgcga catcctcgac atcgacgagt tcgaccgcag
      121 ctggcccacg atgaagctcg atgacctgcg ctaccttgtc tcggcgatca tctccatcgg
      181 ggagaagtcc ggcgagggag agccgccctt ccgcgagtcc caattctacg gcaagtggaa
      241 taccgcgcgg cggcgcatcc tcccacactt caacagcggg gattccgatg ccgaggccaa
      301 gaaaaccaag atcaacagcc cgatcggctg gaaggccctg atggcgaagc tgagccgcct
      361 gcaacggctg cgcatcttcg atcgtgggcg cgaccgcgcc atcgaatacg acgacgaggg
      421 cgcggtcgcg gtcgcagagc tgctcctcgc ccacggcgcc gatgcatcgg tgcggaacac
      481 cgcggggctc accccgctca agtcgcctcg ccgcgcggtc tcgaggaggc ggccgacttg
      541 ctcaatccca gtcagaggcc gtgaagacgg caggaccaca taagccgaac tcggattatg
      601 cgagaccgag agccagcaga tctgcagccg tgcagctcct gctcggtgat tcgaagatcg
      661 acagcttccg ggtgttacgc aagcttggca tgctgcggtc tcccgcgccg gttctcttga
      721 cggcgaagac gccgccacat gtgaaggttt cggacgccgg ccattgcgag ggtgaggcaa
      781 gcgccggcct gcggcaggcc gaaggggcgc cctgggcgcg ccttcggtgg tcgtgcaacg
      841 ccgcccggcc cctgagcgac ctgagcgggg gctagccggt ggcgccgcct ctggggtcgc
      901 cctcgggcag gatttccggg gtggtgaaga ataccccgtc cggggtcggg ttgggggtgg
      961 gcgtcaccgc cacctgcggc ccggccgcgg agagcagctg gatgcgcatc aggagcgcga
     1021 agtcctcgtg cacggtgttg tggcagtggt tgacgtagga gccgccgtac tcgccgaact
     1081 gcacctggaa cgtcacctgc ccgctgtcgc gcagccgcca cacgtccttg cgcaccagca
     1141 gctcggtggc cgggatggtg tcggcacccc ggtccatggt gatgccttcc tcgaagtgca
     1201 ggtggatcgg gtggtcccag ccgccgccct tgttgacgag cgtccagtgc tcgatctcgc
     1261 cgggcttggg gatcagctcg gagatgcggt tggcgttgaa tgagtgggcc ggcccgccgt
     1321 tgacctcgat ggtccacggg aacggcatgc cctccgggca gtcgggcgtg cactgacccg
     1381 tgacgggatc gcgcgagtcg ccgggaccgg agcggccgaa cgcgaccgtg cgggtgcgca
     1441 ccggcgccac gatcgggatc tgctcggtca gcaccgccgg cacctggctc ctgtcgttgg
     1501 gaccgcagct gttggccacg gtgagcgtgg tgcccggctg atcgacgctc ggcaccgagt
     1561 tgacgacccg gaactccatc atcggcccga ccaccggatc gctcagcagg cccgccagcg
     1621 cctcggccag cgacagcgcc ccctgcggcc cgcggccgtc gtcgcgcatc ttgaggagat
     1681 tgacgagctt gatgcgggaa ttgaccggga agatcgagaa gtcgacgacg atgtcgtagc
     1741 gctcggcgat gccctgctcg tcgagcgcgg tcagcgtgat cgggctgacc accaggttgc
     1801 cgtcgttggc gatgaacttg aacggcaccg ccgccccgtt gtaggccagc gccagctggt
     1861 agtagcgcga catcgagccg ttgaggatgc ggaagcggta cttgcgcggc agcacctcga
     1921 agtagggggc ataggcgaag ttgacgagcg gcaggtcgcc caggaagccg tcggtggtga
     1981 agatgtcgaa gaagtactgg ccgtccgggt cggtggtggc gccgtcggag acgacgaggt
     2041 tcacgtcgaa gtcggtattg ccgaagccga gccgggtgcc cgacggcagc cgcaggttgc
     2101 cgcccgaggt gacggtctca tgcccgcggt cgcgcccgga gtagtagttc accatgccga
     2161 ggttgccctt gtagacgttc tcggcggtga agaagaagcg gtggtcgtgc gcccacatgg
     2221 tgccctggat ctcgcgccag tcgcccggca ccttgacgat gcccgagccg ttgtcgggcc
     2281 cggccgcccg ctggtcggtg ccgttgacat tgatcctgtc gcgccgcgcc agcgtggtgc
     2341 tccagcggta gtcgtagaag gtaccgggga agtgatgggc gccggtggcg ccgtcgctct
     2401 ccgccccgtt gtgggcgttg tggaagtgca gctgcgtctc gttgcggccg aagtggttgg
     2461 gaccgttgtc ctcgcggtcg accggcgtgt tgttgtagat gcgggtcagg atcggctcgc
     2521 cgtagcggcc cttgatcagg aacggcggca tgaagccggc gtgcgtgttg ccggtgccgt
     2581 agcaccacac cgagtccggg gcctggtcgg ggaagttggg gtggaacttc gtgccgggcg
     2641 cgcactgcgt ccacgacatc gcgtagccga ccttggggaa gaactcctcc caccgctggt
     2701 gcgcgaagaa ctcgccgggc gggcggccct ccatcggccc gcggttggtc accgggttgc
     2761 ggaacgggtt gtcggcggcc ggcaccggct gcccggtcac ggggtcgaca aagtcgttga
     2821 agtcgttgtg gtaggacagc cgcttggcat tggggtagcc gagcgcggcc gggaacaccg
     2881 cctcgttggc ggcagtgcgc acgagcggga tcggcgtctg caatctcagc cgcggcatcc
     2941 tctgggtgaa cctggcggcg ccgaacaggg ggctccgcgg cgtgccggtc ggcacgtcgg
     3001 caaaggcgct cctggcgaac gggctgaggc cgttcttcaa ggccagcgcg ccggtggccg
     3061 tgaagatgcc ccaccggatg agatcgcgct tggagatctc gcccttcgac aaggccttgc
     3121 ggatctcggc acggttgtcg cgggcgcgct gcgcctccct ctggcgcagg cgggatgctt
     3181 ttggcgaaat atacattcgt gcgttctccc tacgaggctg cgcccgcgtc catcgacccc
     3241 cgcgtcaatc gacccgggcg cccggcctca tgccctagtc ccgggcttct cccaaagtct
     3301 tcaccaccac cgtgttgccg gtgcccttga cgcgcatctt gatgcgctgt cccttgaggt
     3361 cggtcctggc gatctgctcg atgagcttgg gcaccgcgcg ccggtcgccg gccaccgcca
     3421 ccagccacac gtgctcgccc gaggcccact ggtgcggctt gagccgcacc ggctggtcga
     3481 gactgccggc cagcgccttg tcgatctcgc ccgacacccg cgcccacagc gccacggcca
     3541 ccggcaccag cacaccgccc gtcctggttt tatcgggggc tttatcggcg gctttgtcgg
     3601 ggcccttgcc cggaccgagc gacgccggcg cctcggccac cctgaactgg cccgacatca
     3661 ccgccggcag caccagccat tcgaggtcgg cgagcggcat ggtcctgaag ttggggtcgc
     3721 gcatcagcac cgccaccacc tgggcgaacg agcgcgcgaa gcgcgcctgc cgcgcctccc
     3781 gcggccggac ctgggcggcg acctggggtc tggccgggcc ggccatgccc gcctgtgctc
     3841 ccgctgccgc cgcagagcgc gacttgccgg ccccatgcgg gctcgcaccc ggtgtcggat
     3901 tgttcaatat cgtctccctc attgccgggc tcattccccg ggctcatttc ccgggctcat
     3961 tcccggactc cggcggacac tcgcgcgata gtccctcgaa cgtccgccgg ccgtgctcag
     4021 tgcccgtgct cagtgcccgg gctcagtgcc cggactcagt gcccgtgctc agtgcaggat
     4081 gaagtcttgc tgggtgatgg tgttggcgcc cacgccgttg acacccagca gcaggatcgt
     4141 gtcgccgccg atggcgacca gcgtgtcgtt gccgagatcg gtgatcagga cgctggctgc
     4201 aaaattggcc gccgtgatgc cgagcgccga gatgtcaagc aggtcctggc cccccgtcgg
     4261 gttggcgtcg aagcccgaga tcgtgtcgtt gccgaacccg gccgcgaaca cgaagctgtc
     4321 gttgccggtg ccgccgttca tcacgtcgtt gccggcgccg ccgatcagcg tatcggcgcc
     4381 ggcgccgccg tcgagcgaat cgacgccgcc gttgccgatc agggtgtcgt tgccgccgcc
     4441 gcccgccagc acgtcggtgc cgccggcccc ggcgatgacg ttgtccgacc cgttgccggt
     4501 gccggtgaag ccgccggcgc cggtgaaggt caggttgtcc acgttggcgg ccagcgtgaa
     4561 gctggcactg gaggtgagca cggagtcgat gccggcaccg gccgcctcgg tgatcgtgtc
     4621 accgttgtcg gcgaggtagg tgtcgttgcc ggcaccaccg gccaggttgt tgaccgccgc
     4681 attgccggcc ccgctcagcg tatcgttgcc cgatccgccg gtgacgttgt cgatgccggc
     4741 gatggcggcg aagcccgatg ccgtccccgc cgccaggttg actgtcacgt ccgccgtcga
     4801 cccagcgtag gacagccggt cggtcccgcc caggaggtcg gcggtgatcg cctcgacact
     4861 tgtcaccgtg cccagctcga gcccggtgag cgaaacgccg tcgaagatca cgtcgagcgt
     4921 gtcgttggcg gcggtgccgg tgacgctcag cgtgtcggtg ccggcgccgc cgtcgaccgt
     4981 gtcggcgccg tcgccgaagt tgaagatgaa gctgtcgttg ccgaggccgc ccgacaggat
     5041 gtcgttgccg gtgccgccgg tcagcgtatc gtcgccggct ccgccgctga cggtgtcgtt
     5101 gccgccgcca gcgttgatcg tgtcattgcc gccctggccg tcgatgacat tgacgccgcc
     5161 gttgccggtg atggtgtcgc tgccctggct gccgatcacg ttctcgacgc cggccagcgt
     5221 gtcggtgccg gtctcggcgc tggtcgaggt ggtggccgtg accaccgcgc cggccgcggt
     5281 cgccgacagg tcgtaggtgt cgatgccggc accgccgttg taggcatcgt tgccgtcgcc
     5341 gacgccggcc accagccggt cgtcgtcggc cccgccgttc accgtatcgt cgccggcccc
     5401 gccggtcagc gtatcggcgc cgacattgcc gttgagcgtg tcgttgcccg ccccgcccag
     5461 gagcgtgtcg ctgtcgaagc cgccgttgat ggtgttggca agatcgttgc cggtgccggt
     5521 gaagccgccc gcgccgatga aggtcaaggt ctcgacgttg gccgtcagcg tgtagctgct
     5581 gagatcggtc tgcaccgtgt cggtgccctc gcccgggttc tcgagcacct ggtcgccggc
     5641 gttgtcgacg aagtagatgt cgttgccgag cccacccgac atggtgtcgg cgccgaggcc
     5701 gccgtcgagc cggtcgttgc cggcgccgcc gtcgagcagg tcgttgccgg ccagcccgtt
     5761 gaggatatcg gcgccgccct ggccgaagat ctcgtcctgc ccgtcggtgc cgatgaaggt
     5821 gtcgggaccg ttggtgccga agatcaggtc gccgaccact tgcgtggccg ccgaggccac
     5881 cgtctccacc gtgccctggt cgtcggtgta ggtgaccagc acccgcagct gcaggcccac
     5941 ctgcgcctgc gtcggcacga acagctggcc ggtgccgtcg gtcaggtcgc cgacggcatc
     6001 cacccacgtg atgccatccg ccgactgctg ccagcggaag gcaaacagcc cgcccgcaac
     6061 cgccgtggcg gtcccgtccg gatcgacgat ggtggccgga tccaccgtca gcgcccgccc
     6121 ctccgtcggc gtggtgtcgc tgatcgtcgg cccggccgtg ggcagatcgt tgacattggc
     6181 gaccg
//
LOCUS       DATNBV010000543         2058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1596241,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000543 DATNBV010000000
VERSION     DATNBV010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596241"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VN627_12890"
     CDS             complement(<1..359)
                     /locus_tag="VN627_12890"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406925.1"
                     /note="NADP-dependent; catalyzes the oxidative
                     decarboxylation of malate to form pyruvate; decarboxylates
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HXD04192.1"
                     /translation="MSSQNDGHGGRPTFTDQEALLFHSQGRPGKLEVVATKPMATQRD
                     LSLAYSPGVAVPVLAIAENEDLAFDYTTKGNFVAVITNGTAILGLGNRGALAAKPVME
                     GKAVLFKRFADIDSIDL"
     gene            605..928
                     /locus_tag="VN627_12895"
     CDS             605..928
                     /locus_tag="VN627_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04193.1"
                     /translation="MIRFVFRFAGLVALALGFIFLVHDGTKSIADQTLYLSSVSSTWE
                     NVHQSSLAALQPVVERYAGTWGWQGVVKPYFLDQPVSLVLAIIGAILILLGRKKKPLI
                     GYARD"
     gene            complement(982..1554)
                     /locus_tag="VN627_12900"
     CDS             complement(982..1554)
                     /locus_tag="VN627_12900"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HXD04194.1"
                     /translation="MRRVVVTLFAACAALAMPGAAWAQAPAPAPAPAPAAPAAPAAPA
                     AVPDQMPFDVPYGASITADRAAQVVAAAVAEAQKSPRNWKLAISVVDPNGDLVYFYKM
                     DQTQAASVGISQGKARTAARFRRPSQVFFNVMQNPAGAFAGTLDPTLVASPGGFPLVE
                     GGKIIGAIGCSGATGDQDAVACKAGADTIK"
     gene            1797..>2058
                     /locus_tag="VN627_12905"
     CDS             1797..>2058
                     /locus_tag="VN627_12905"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HXD04195.1"
                     /translation="MFMFKKKLEMPSAAEALPGRSTPIPTAREHFVLHRPIKGPYPEG
                     LETAMFGLGCFWGAERKFWELSDGIYATAVGYAGGPTPNPTYE"
BASE COUNT          363 a          648 c          680 g          367 t
ORIGIN      
        1 tcgaggtcga tcgaatcgat gtcggcgaag cgcttgaaca gcaccgcctt gccttccatc
       61 acgggcttgg ccgccagcgc gccgcgattg cccaagccca aaatcgcagt gccgttggtg
      121 atgacggcga cgaaattgcc cttggtggta taatcgaagg cgaggtcctc gttctcggcg
      181 atggcgagca ccggaacggc gacgccgggc gaataggcca gcgacaggtc gcgctgggtc
      241 gccatcggct tggtggccac gacttcgagc ttgcccgggc ggccctggct gtggaacagc
      301 agggcttcct ggtcggtgaa agtgggccgt ccaccgtgac cgtcgttttg tgaagacatc
      361 tcaacccctt acaagaactt gcgtagagcg gctggagagc cgaaggtaat ccgtcttatc
      421 tcccccgcgt gcgggaaagt catgaatcca tcacccccca cctgcgccat catgctggcg
      481 tgggtaaggg ctcgtccaca gcgataggtt gtccggaccg ggaataattg aacggcgagg
      541 gcgagcgcgc taatcggtag cgcgaagacg cacatttctc cagggcgtag aacggacacg
      601 cgccatgatc cgctttgtat ttcgtttcgc cgggctggtg gcgctcgcct tgggtttcat
      661 cttcttggtc cacgacggca ccaagtcgat cgccgaccag accttgtatc tcagcagcgt
      721 gagctcgacc tgggaaaacg tgcaccagag ctcgctcgcg gcgttgcagc ccgtggtcga
      781 gcgttatgcc gggacgtggg gatggcaggg cgtggtcaag ccctactttc tcgaccaacc
      841 ggtctcgctc gtgctcgcca tcatcggcgc catcctgatc ctgttggggc ggaagaagaa
      901 accgctgatc ggatacgccc gcgactgaaa caatatggac cgcggctgac gccgcggtcc
      961 gttcggtccc ttcggcgcag attacttgat ggtgtccgcg cccgccttgc aggcgaccgc
     1021 gtcctgatcg ccggtggcgc cgctgcagcc gatcgcgccg atgatcttgc cgccttcgac
     1081 gagcgggaag ccgccgggag aggccaccag cgtcgggtcg agcgtgccgg cgaaggcacc
     1141 ggccggattc tgcatgacat tgaagaagac ctgagatggc cgccggaagc gggccgccgt
     1201 acgggccttg ccctgtgaga tcccgaccga ggcagcctgg gtctgatcca tcttgtagaa
     1261 gtagacgaga tcgccgttcg gatcgaccac cgagatcgcg agcttccagt tgcgcggcga
     1321 cttctgggct tcggccaccg cggcggcgac cacctgcgcg gcacgatcgg cggtaatcga
     1381 cgccccgtat ggaacatcga acggcatctg atcaggaacg gccgccggtg ccgcgggcgc
     1441 ggccggtgcc gccggcgctg gggccggcgc gggtgcgggc gcctgcgccc acgcggcgcc
     1501 aggcatcgcg agcgcggcac aggcagcaaa caaggtgaca acaacacgac gcattttaag
     1561 tcctcccact ttggtttaat cgccgtcctt gctggcacgg cgttcatacg cgcgtcagca
     1621 tacgggcttg cagtcgggcg cgaaacgggg ttgtcggcct cgatgcggcc cgcgcgctcg
     1681 tgtgaccccc gcggcgggtc ggttcgacgc gcagtgcggg caatggagac gcgagacagg
     1741 tgcgccgccc ctcgaccgca cctatattgg tgtcacgatc acgggagaac gatgccatgt
     1801 tcatgttcaa gaagaagctt gaaatgccgt cggccgccga ggcattgccc ggccgttcga
     1861 ccccgatccc gaccgcgcgg gagcacttcg tccttcaccg tccgatcaag gggccgtatc
     1921 cggaaggtct cgaaaccgcg atgttcggcc tgggttgctt ctggggcgcc gaacgcaagt
     1981 tttgggaact gagcgatggc atttacgcca ccgctgtcgg ctatgccggc ggacccacgc
     2041 caaatccgac ctatgagg
//
LOCUS       DATNBV010000544         3806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2213618,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000544 DATNBV010000000
VERSION     DATNBV010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213618"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VN627_12910"
     CDS             complement(<1..278)
                     /locus_tag="VN627_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXD04196.1"
                     /translation="MSAPLPLRNIAIIAHVDHGKTTLVDQLFRQSGTFRDNQRVEERA
                     MDSNDLEKERGITILAKCTSVEWTNPGTNETTRINIVDTPGHADFGGEV"
     gene            583..3264
                     /locus_tag="VN627_12915"
     CDS             583..3264
                     /locus_tag="VN627_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXD04197.1"
                     /translation="MIISDSRFAVASRHALAWGIALAALAAPVAAQAQAQAQAEAAPP
                     PEEAQPAPPADDADASGNEIVVTATKREQTLQDVPVAVTVTTAVTIERAQIRDIRDLS
                     TVVPSLRVNTLQSSASTNFYIRGFGNGANNAGIEPSVGLFVDGVYRSRSASMISDLPD
                     VQRIEVLRGPQSTLFGKNASAGVISLVTKEPKFTLGGNVEASYGNYNAVVAKGYVTGP
                     ISQTVAASLAAGYNKRDGYVTDTGTGTKSNERNRWFVRGQVLIKPSADLKVRLIGDYS
                     KIDEVCCAAINLQRSPGLGASQVVELLGGQLSDPAAPYADRVAYNFPSTNKIDNWGLS
                     GQVDYSAGILKFSSITAFRHTGAITNQDSDFSAADLLRRNYQDLQIDTFTQELRASTD
                     WDGPFNALLGAFYFNEKIKQHNELQYGSQFQAYANGLIQGATGCFNTPVSLACNNLAG
                     LSGALGAVDGSGSTLFRAGQGMDENYALKDESWSAFAQFDYRVNDRLTLTGGINYTHD
                     AKRFSTATTSSDVFSAIDLDAPALAPLRAQLLAAGALDKGATLQQAQLFAAAYMNDPT
                     ANPLNDLKAFQFMPQFLNVPNALETGRIGDGNWSFTARAAYDLTDRVNVYASYATGYK
                     AASVNLSRDSRPVASVQAAFAGNPLYPNLTVGSRFADPEDSAVWEAGLKGNWGNATAN
                     LAVFKQSIKGFQSNIFTGTGFKLANAGKQSAWGVEFEGMIKPTPELTFNVGMTYLDPK
                     YDDFRQSGVGDITGTRPGGISSFTATIGGQWDHELGNGDHVILRGDYHYEAPFALVEG
                     LPNLVVKVKNAVGQVTGLDYTAAIAAATSRKQEIKLANASLTYAMHSGLELTLWGRNI
                     TNNRTILQIFDSPVQPGSISAYPSEPRTWGGSVRYRF"
     gene            3640..>3806
                     /locus_tag="VN627_12920"
     CDS             3640..>3806
                     /locus_tag="VN627_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HXD04198.1"
                     /translation="MEWMLMPLRRYAEFSGRSRRKEFWMFVLFMMLVYAACGVLMLGG
                     GLGASLATFNTG"
BASE COUNT          680 a         1260 c         1217 g          649 t
ORIGIN      
        1 acctcgccgc cgaaatcggc gtggccgggc gtgtcgacga tgttgatgcg ggtggtttcg
       61 ttagtgcccg ggttggtcca ctccaccgag gtgcacttgg cgagaatggt gatcccgcgt
      121 tccttttcga gatcgttcga atccatcgcc cgctcttcga cgcgctggtt gtcgcggaag
      181 gtgccggact ggcggaaaag ctggtcgacc aaggtggtct tgccgtggtc gacgtgggcg
      241 atgatcgcga tattgcgcag gggaagcggg gcggacatgg gacttgaggt ctttcggctg
      301 gcggcggggc cgcatattcg ctgcggcgca acaattgcgc gcccgtagcc gaaccggcgg
      361 attcgggcaa gcatggcgac cgccacgcgg cgcatgcgaa tggaatgcat tgttgctgca
      421 cggccacaag gccctgtccc catgggaatg gaggcgattg ccgttgcctc gggccggcgg
      481 gattcctatg gtttgggaca agcacgaagg ggctgcgtca attcggtggc aagggatcgc
      541 cgccaacacc ttgccgcagt gcaccgggag agggaccgcg ccatgatcat ttccgacagt
      601 cgttttgccg tcgccagccg ccacgccctg gcttggggca tcgccctcgc cgcgctagcc
      661 gcaccggtgg ccgcccaggc ccaggcccag gcccaggccg aggctgcccc gccgcccgag
      721 gaagcacagc ctgcccctcc tgccgatgac gcggatgcca gtggcaacga gatcgtggtt
      781 accgccacca agcgcgagca gaccctgcag gacgtgccgg tggccgtcac cgtcaccacc
      841 gccgtgacga tcgagcgtgc ccagatccgc gatatccgcg atctttccac cgtggtgccc
      901 tcgctgcggg tcaacacgct gcaaagctcg gccagcacca atttctacat ccgcggcttc
      961 ggcaacggcg cgaacaatgc cgggatcgag ccttcggtcg gcctgttcgt cgacggggtc
     1021 taccgctcgc gctcggcctc gatgatctcc gacctgcccg acgtccagcg gatcgaggtg
     1081 ctgcgcgggc cgcagtccac cctgttcggc aagaatgcct cggccggcgt gatctcgctg
     1141 gtgaccaagg aacccaagtt caccctcggc ggcaatgtcg aggcgagcta cggcaactat
     1201 aacgccgtgg tcgccaaggg ctacgtgacc gggccgatct cgcagacggt cgccgccagc
     1261 ctcgccgccg gctacaacaa gcgcgacggc tatgttaccg ataccggcac cggcaccaag
     1321 tcgaacgagc gcaatcgctg gttcgtgcgc gggcaggtgc tgatcaagcc ttcggccgat
     1381 ctcaaggtcc ggctgatcgg agactacagc aagatcgacg aggtgtgctg cgccgcgatc
     1441 aacctccagc gctcgcccgg gctgggtgcg tcgcaggtgg tcgagctgct cggcggccag
     1501 ctgagcgatc cggctgcgcc ctatgccgac cgggtggcct acaatttccc gtcgaccaac
     1561 aagatcgaca actggggcct ctccggccag gttgactaca gcgccggcat cctgaagttc
     1621 agctcgatca ccgctttccg ccacaccggg gcgataacca accaggacag cgatttcagc
     1681 gcggccgatc tgctccggcg gaattaccag gacctgcaga tcgacacctt cacgcaggaa
     1741 ctgcgcgcct cgaccgattg ggacgggccg ttcaatgcgc tgctgggcgc cttctacttc
     1801 aacgagaaga tcaagcagca caacgagctg caatatggca gccagttcca ggcctacgcc
     1861 aacggtctca tccagggcgc gaccggctgc ttcaacacgc ccgtctcgct cgcctgcaac
     1921 aaccttgccg gcctgtccgg agcactgggc gcggttgatg gcagcgggtc aaccctgttc
     1981 cgcgccggcc aggggatgga cgaaaactac gcgctgaagg acgaaagctg gtcggccttc
     2041 gcccagttcg actatcgggt gaacgaccgc ctgacgttga ccggcgggat caactacacc
     2101 cacgatgcca agcgcttctc caccgccacc acgtccagcg acgtgttctc ggcgatcgat
     2161 ctcgacgccc cggcgctggc gccgctgcgg gcccagctgc tggcggccgg cgcgctcgac
     2221 aagggggcga ccctgcagca ggcccagctc ttcgccgcgg cctacatgaa cgatccgacg
     2281 gccaatccgc tgaacgacct caaggcgttc cagttcatgc cgcaattcct caacgtgccc
     2341 aacgcgctgg aaacggggcg gatcggcgat ggcaactggt cgtttaccgc ccgcgcggcc
     2401 tacgatctca ccgatcgcgt caacgtctat gccagctatg cgaccggcta caaggccgcc
     2461 tcggtcaacc tctcgcgtga cagccggccg gtggcttcgg tgcaagcggc ctttgccggc
     2521 aatccactct accccaacct caccgtcggc agccgctttg cggatcccga ggattcggcc
     2581 gtgtgggaag ccgggctcaa ggggaactgg ggcaatgcca cggccaacct tgcggtgttc
     2641 aagcagtcga tcaaggggtt ccagtcgaac atcttcaccg gcaccggctt caagctggcc
     2701 aacgccggca agcagagcgc ctggggcgtg gaattcgagg gcatgatcaa gcccaccccc
     2761 gaactcacct tcaatgtcgg catgacttac ctcgatccca agtacgacga tttcaggcag
     2821 tcgggcgtgg gcgatatcac cggcacccgg cccggcggca tctccagctt caccgccacc
     2881 atcggcggcc agtgggatca cgagctcggc aatggcgacc atgtgatcct gcggggcgac
     2941 tatcactatg aggcgccctt cgccctggtg gaggggctgc ccaacctggt ggtgaaggtg
     3001 aagaacgccg tgggtcaggt gacgggcctg gactacaccg cggccatcgc cgcggcgacc
     3061 agccgcaagc aggagatcaa gctggccaat gcctcgctga cctatgcgat gcacagcggg
     3121 ctggaactaa ccctgtgggg ccgcaacatc accaacaacc gcacgatcct gcagatcttc
     3181 gattcccccg tacagcccgg ctcgatctcc gcctatccaa gcgaaccgcg gacctggggc
     3241 gggtcggtgc gctatcggtt ctgagggggg cggggaaggc gactggccgc acccgcaagt
     3301 gggtcacccc gtcatcccgt caccccgtca tcctgaacgt gtttcaggat ccacggagcg
     3361 accctgcctg atagacggcg ctcgccatta agagcgcccg ccatgccgat tgaatcgcgc
     3421 catgagcgca gcgcttcatc cccgacactg cgtggcgggc gatgcttgcg caatggatcc
     3481 tgaaacaagt tcaggatgac gggttcttgt agggccgttg ctcacgatcc gatccggcct
     3541 tgtctcctcg cccgccaagg ggcaccatgc atgaaggtcg cggatcgtag acagctcgag
     3601 ggcaatggcg ccgggcatgt ttggacaagg gggcaagcca tggaatggat gctgatgccg
     3661 ctacgacgct acgccgagtt ttcggggcgg tcgcgccgca aggaattctg gatgttcgtg
     3721 ctgtttatga tgctggtcta tgcggcctgc ggggtgctga tgctgggcgg cgggctgggc
     3781 gcttcgctgg cgacgttcaa cacggg
//
LOCUS       DATNBV010000545         3644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3119788,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000545 DATNBV010000000
VERSION     DATNBV010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3119788"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VN627_12925"
     CDS             <1..872
                     /locus_tag="VN627_12925"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXD04199.1"
                     /translation="DADVVLLLGSRAITEASPRPAPYFPKARFVAQVNADPSRLEAIR
                     RFDWVSASDSGAFATALLAALEAGPAPSAEIRAARADWLARPTPPLPRSGSLGRLFAG
                     FARCVAPLHDALDHGWLVDETVMAAGVLQSALTAKDGRRYMGTNGASLGWGTGAASGV
                     ALASGEPVTCTLGDGSLRFGMAGLWTARAMNLPITFIIMDNHGYGSTRNYERQHIAKL
                     GPDARPQKPGYFNMDMRTLGPDIQGMVEGFGIPCRRLSPEDDLRAAVLDAWARRSDGP
                     NALIMPVGFEDED"
     gene            884..2275
                     /locus_tag="VN627_12930"
     CDS             884..2275
                     /locus_tag="VN627_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXD04200.1"
                     /translation="MADLTFPERPDHSAGIADFAAIFGEAFTTSRDVCKVHSNSSGIR
                     RPSDGVLFASTTEQIVEALRLCSHHRMPLIPWGAGTSVDGNTAAWLGGVSLDLSRMDQ
                     VLRVSPEDQDCTVQAGVTRENLNKYLRDTGLFFPIDACPQASMGGMASLRASGTTAVR
                     YGTMREQVLGLKVVLANGEVIRTGGRARKSAAGYDLTHLFVGAAGTLGVITEVTLRLQ
                     GIPEHLLAATCAFPDLDAAMNAVIGTIQMGVPMARIEIMDALQIKFCNAFMNLGLPEL
                     PHLFMEFDGLPEANAAYSALVREIAEGCGGTGFEASSNREERERIWTARHNNGASAMH
                     VHPGTMGLATDVTVPISRLAECLTETHADLAKIDVPGPISGHVGDGNFHVILLLEPNN
                     PEHMEQANWFMDRLIERALRMDGTCSGEHGMGLAKKHWAEREFGAGGVEAMRMIKRTF
                     DPLNILNPGKIFD"
     gene            complement(2272..3087)
                     /locus_tag="VN627_12935"
     CDS             complement(2272..3087)
                     /locus_tag="VN627_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXD04201.1"
                     /translation="MSATSLHPRLSLNGIVTPAWSFEQDLAFWRECGLSCVGLLHPKV
                     MAHGPDRVAAALKSEGLTVSTIICGVFTLEQPECWPAERARLDAMIDLAAEVGGTVYG
                     PPGRGWFDQWDANCAAYAEAVAPCLAHARERGVTLAFEPTLRPALSFVHTLRDGLDLC
                     AVSGAAMVVDIGNCCTERDHRATIRAMPINRMGLVQISDLDAGTPAAPGMGIRVLPGE
                     GDLPLEDHIRAAHEAGYAGAYEIELLGEAEPDRAAIRRSLELVSAMLDRVVGR"
     gene            complement(3091..>3644)
                     /locus_tag="VN627_12940"
     CDS             complement(3091..>3644)
                     /locus_tag="VN627_12940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD04202.1"
                     /translation="ILMNNAGVVYSGPFLEVDMALWRKSFNVNMFAVVEATQLMLPDI
                     LAAGGGHIVNVATTAALYPYNADRMSYNSARAGVVLFSESLAMDLWEKGVGVTCLCPG
                     PTRTNMIQNMHFVGEPAITTPDLPIMEPEELADKLVKAIRGGKFFLPGHDEVRAIVAR
                     RGADVDAFLTDAAEKFNAGRKGR"
BASE COUNT          596 a         1179 c         1260 g          609 t
ORIGIN      
        1 aggatgccga cgttgtgctg ctgctggggt cgcgggcgat taccgaggcg agcccgcggc
       61 cggcacctta tttccccaag gcgcggttcg ttgcccaggt caatgccgat ccgtcgcggc
      121 ttgaggccat tcgccggttc gactgggttt cggcaagcga ttccggcgcc ttcgccacgg
      181 ccttgctggc cgcgctcgag gcgggccccg cgccatcggc cgagatcagg gctgcccgcg
      241 ccgattggct ggcgcggccc actccgccat tgccgcgttc gggctcgctg ggccggctgt
      301 tcgccgggtt cgcccgctgc gtcgcgccgc tccatgatgc gctggaccat ggctggctgg
      361 tcgatgaaac ggtgatggcg gccggcgtgc tgcaatcggc gctcacggcc aaggacgggc
      421 ggcgctatat gggcaccaac ggcgcgtcgc ttggctgggg cacgggggcg gcttccggcg
      481 tggccctggc ttcgggggag ccggtgacct gtacgctcgg tgatggcagc ctgcgcttcg
      541 gcatggccgg gctgtggacg gccagggcaa tgaacctgcc gatcaccttc atcatcatgg
      601 ataatcacgg ctacggctcg acccgcaatt acgagcgcca gcacattgcc aagctcggcc
      661 ccgatgcccg gccgcagaag ccgggctact tcaacatgga catgcgcacg ctggggccgg
      721 acatccaggg catggtggaa ggcttcggga taccctgccg gcggctcagc ccggaggacg
      781 acctgcgcgc agccgtgctc gacgcctggg cgcgccgcag cgacgggccc aatgccctga
      841 tcatgccggt aggcttcgag gacgaggact agggagagcg cagatggccg accttacttt
      901 tcccgagcgc ccagaccata gcgccggcat tgccgacttc gccgcaatct ttggcgaggc
      961 gttcaccacc tcgcgcgatg tctgcaaggt tcactccaac agcagcggca tccgccggcc
     1021 gagcgacggg gtgctcttcg ccagcacgac cgagcagatc gtcgaggcac tgcggctgtg
     1081 ttcgcatcac cggatgccgc taatcccgtg gggcgcgggc acttcggtgg atggcaacac
     1141 ggccgcctgg ctgggcgggg tctcgctcga cttgtcgcgg atggaccagg tcctgcgggt
     1201 ctcgcccgag gaccaggact gcacggtcca ggccggggtt acgcgcgaga acctcaacaa
     1261 gtacctgcgc gataccggtc tgttcttccc catcgatgcc tgcccgcagg cctcgatggg
     1321 cggcatggcc agcctgcgcg cctcgggcac gacggccgtc cgctatggca cgatgcgcga
     1381 gcaggtgctg ggcctcaagg tggtgctggc caacggcgag gtgatcagga ccgggggcag
     1441 ggcgcgcaag tctgcggctg gctatgatct tacccacctg ttcgtgggcg cggcgggcac
     1501 gctgggagtg atcaccgagg taaccttgcg ccttcagggt attcccgagc atctgctcgc
     1561 cgcgacctgt gcctttcccg acctggatgc ggccatgaac gcggtgatcg gcacgatcca
     1621 gatgggcgtg ccgatggccc ggatcgagat catggacgcc ctgcagatca agttctgcaa
     1681 cgccttcatg aacctggggc tgcccgagct gccgcatctg ttcatggagt tcgacgggct
     1741 gcccgaggcc aatgccgcct attccgcgct ggtcagggag atcgccgagg gctgcggcgg
     1801 caccggtttc gaggcatcgt ccaaccgcga ggaacgcgag cggatctgga ccgcccggca
     1861 caacaacggg gcctcggcga tgcatgtcca tcccggcacc atggggctgg ccaccgacgt
     1921 gaccgtgccg atctcgcgct tggccgaatg cttgaccgaa acgcatgccg accttgcaaa
     1981 aatcgatgtc cctgggccga tctcgggcca cgttggcgat ggcaacttcc atgttatcct
     2041 gctgctcgag ccgaataatc cagaacacat ggagcaggcc aactggttca tggaccgcct
     2101 gatcgagcgg gccctgcgga tggacgggac ctgctcgggc gaacacggca tgggcctggc
     2161 caagaagcac tgggccgagc gggaatttgg cgcgggcggg gtggaggcga tgcggatgat
     2221 caagcggacc ttcgatccgc tgaacatcct caatcccggc aagatcttcg attaacgccc
     2281 gacgacccgg tccagcatgg cgctgaccag ttcgaggctg cgcctgattg cggcgcgatc
     2341 gggctcggcc tcgccaagca gctcgatttc gtaggcgccg gcatacccgg cctcgtgggc
     2401 cgcgcggata tggtcctcca gcggcagatc gccctctccg ggcaggacgc ggatgcccat
     2461 gccgggggct gcgggcgtgc ccgcgtcgag gtcgctgatc tggaccaagc ccatgcggtt
     2521 gatcggcatg gcgcggatcg tggcgcggtg atcgcgctcg gtgcagcaat tgccgatatc
     2581 caccaccatc gccgcgccgc tgaccgcaca caggtcaaga ccgtcgcgca gcgtgtgcac
     2641 gaacgacagc gcggggcgca aggtcggctc gaatgcgagt gtcacgcccc gctcgcgcgc
     2701 atgagcaagg cagggggcca cggcctcggc ataggccgcg caattggcat cccattgatc
     2761 gaaccacccc ctgccgggcg ggccatagac cgtgccgcca acctcggcgg cgaggtcgat
     2821 catggcgtcg aggcgggcgc gttcggcggg ccagcactcg ggctgctcaa gcgtgaagac
     2881 cccgcagatg atggtcgata cggtgaggcc ctccgacttg agagcggcgg cgacccggtc
     2941 tggcccatgg gccatgactt tcggatggag cagcccgacg cagctcaggc cgcattcgcg
     3001 ccagaaggcg aggtcctgct cgaaggacca ggccggggtg acgataccgt tgagtgagag
     3061 ccggggatgg agcgacgtgg cgctcatcgc tcagcgaccc ttgcggccgg cgttgaactt
     3121 ttcggccgcg tcggtgagga aagcatccac gtctgcgcca cgccgggcaa cgattgcgcg
     3181 cacctcgtca tggcccggga ggaagaactt gccgccgcgg atcgccttga ccagcttgtc
     3241 tgccagctcc tcgggttcca tgatcggcag gtccggggtg gtgatcgcgg gctcgccgac
     3301 gaagtgcatg ttctggatca tgttggtgcg cgtcggtccc gggcacaggc aggtgacgcc
     3361 gacgcccttc tcccacagat ccatggctag gctttcactg aacaggacca cacccgcgcg
     3421 ggcggaattg tagctcatcc gatcggcgtt gtagggatag agagctgcgg tggtggcgac
     3481 gttgacgatg tggccgccgc cggcagcaag gatatcgggc agcatcagct gcgtggcttc
     3541 gacgaccgcg aacatgttca cattgaacga tttgcgccac aacgccatgt ccacctcgag
     3601 gaacgggccg gaatagacta ccccggcatt gttcatgagg atgg
//
LOCUS       DATNBV010000546         6815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2516671,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000546 DATNBV010000000
VERSION     DATNBV010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2516671"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VN627_12945"
     CDS             complement(<1..451)
                     /locus_tag="VN627_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HXD04203.1"
                     /translation="MEFSRRRVLGALAVGGASLAFPRSGLQDAYAQDLPPPAGAIPGF
                     SEMGGVRPLSLSETATGQARLTSVLLPQARAALDRHGYAIGNRQRIGLVDFSAPSKSA
                     RFYLIDLPAGEVVSSHLVAHGRGSDPANSGWVERLSNRPGSNASCEGA"
     gene            752..3862
                     /locus_tag="VN627_12950"
     CDS             752..3862
                     /locus_tag="VN627_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXD04204.1"
                     /translation="MVRTAARVWRRGIAGCAWLAAASGIVGAGLANAAPRDALRAPSY
                     AESFRIGSSGPLCEAQAVAAGSPRGSMFDRRWALLCREVARPVGTAAFVRGGAPAAPG
                     DGLDEPLDCAEPARVAVAGLSGVVARDCRGRTSGAAYRSYALRTGRGGWSVAGLAAFD
                     SALKLTLRSLVADRLVPGAIEAVTLGTGGAGGFFQSKAAAADAESLLGQGYRRNAAGA
                     YAEAAQIFAATAAAIGAGNADSAARRHELVINQALQLSNLGQFDQAAVLFAQARAMPG
                     IDFVQARLARNFEAIDALNRRDVAAVEGILDRPAPPVTAGVIAGDGAAEIDPATAAGL
                     ATGTAPNLARILGQDVRLTPAERAAIADAQALALRGTALRLSGKPAEARTALEGANRA
                     ALAVRDGRVVSVTRLRSQILAEIGQAWESEGKPAEAERLYREVLLLVATQYPDSASVN
                     SAKARLAGFLVRQGRIDAGRADYKALVTGVIGERDALVGMANLIQPYFDLLVANGATS
                     EADLGELLMASQLVQRPGAADTLSQLSRQLEAGNDDAAALFRRSLAVGRDIERARVQQ
                     ARLATAEGDKTLLAAQAADLAAQQARLEEAQLQLVSQLSAFPQYRAISRSYVTLPELR
                     ATLGTGEGYLKLVTLGERTYALLVTPSGGRGWLVGKSAAELADMVTALRESISVTVNG
                     VRSTYPFDIDTDLALSQALLGPARADLAGIRHLVFEPDGAMLQLPANLLVIDPAGADA
                     YRARLAAGGDEFDMTGVAWLGRTTAISTALSAASFRDSRAAPASKAAHAYLGLGHNQP
                     LGAVTTLPSVRGVAQGSPFAQGCDWPVATWNQPIAATELQQAAGLFGPANAALMTGAA
                     FTDTAIETRPDLADFRVVHFATHGLVTAPRTGCPARPALVTSFGGNGSDGLLRFDEIF
                     NLHLDADLVILSACDTAGGASLDATREAGETSGGGQALDGLVRAFIAAGGRQVIASHW
                     PAPDDFNATERLVGGLFAATGEESLAEALRLSEVALMDSADTSHPFYWAGFALIGDGR
                     RPLHAR"
     gene            3938..5905
                     /locus_tag="VN627_12955"
     CDS             3938..5905
                     /locus_tag="VN627_12955"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXD04205.1"
                     /translation="MRGAGGRRLSATGAALLIALFLAIFAWRVPGLGDAERSLYDLRS
                     YLAAPKVAQDPRVLLVVYDDQTLIAARKRSPLDRGLLAKALANLDAMGAKAIGIDILF
                     DQPQDEDDALVAALRGMKTPTFVAYANVAGNRDDIVYDQQQYLDAFQRRLVGSNAHPA
                     SIRLDFAQGATRRWPEIVPSLPPVLARAMLAAGGDQGAVQRFAGYRGPVRYRLPESAD
                     RPVFTSLKIDLFADPEIAAAMADQVRGRYVILGGDIVDVDRVPTTFTSVDGEAVPGLQ
                     VHAAMISQMLDGAAQGRVPPLQRAAFAVLLVVAATLTSLLEWQPWRLVPFILVQAVGF
                     GGVPFLLQARGVDTWDLPAAGWALAWIIAFGAVASAARASTAAQRRFAHDALGKYLPR
                     DIAREIIEKPELLALHGEKKPLFIVFSDLEGFTKLSHDLPPETVARLLNQYLEMLSKV
                     VLDHGGVIDKFVGDAVVAFWGAPVARPNDGERAARAAYAMWQAGEEFRRINAVEGLPP
                     IGKTRVGLHHGEAVVGNFGGERRIQYTALGDAMNTAARLESANKPLAASVMASREAAD
                     LSGLDWWRPMGKVVLRGRTRAVELREPAPDFPAEDRAALAEALAAFEADRAAGLAQLR
                     LIAARHPADAALANLVTRCESLGERDAYVLD"
     gene            5886..6614
                     /locus_tag="VN627_12960"
     CDS             5886..6614
                     /locus_tag="VN627_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04206.1"
                     /translation="MPMFSINYLAAGAAALALAIPAAAEAAIVVASSGPSAATYPVGR
                     KLDDAGTITLRAGDTLTVLDGKGTRVLRGAGNFPVAAAAGTPAKSATFAVLTMQRSAQ
                     RVRTGAVRTGVGAEAPASPNLWFIDVAKPGKRCLVAGQPVRLWRLDTKAAATYTLAPA
                     AGGAGAAVTFAAGSGVAPWNTVAIPVTDGGRYVVSAGGKPVGTVETALLPAQPRDAEA
                     MAAALIGKGCTAQVELLAATLGQQ"
BASE COUNT          945 a         2573 c         2251 g         1046 t
ORIGIN      
        1 aagcgccctc gcacgaggca ttcgagcccg gccggttgga cagccgttcc acccagccgg
       61 aattggccgg gtccgaaccg cggccgtggg cgaccagatg gctcgagacc acttcaccgg
      121 cgggcaggtc gatcaggtag aagcgggctg atttcgaggg cgcggagaaa tcgacgaggc
      181 cgatccgctg gcgattgccg atggcatagc cgtggcgatc gagcgccgcg cgcgcctggg
      241 ggaggagcac tgaggtgagc cgggcctgcc cggtggccgt ttccgaaagg gagagtggcc
      301 gcacgccgcc catttccgaa aaaccgggaa tggcgccggc cggtgggggc aggtcctgcg
      361 cataggcatc ctgcagcccc gaacggggga aggccaggct ggcgccgccg acggcgagtg
      421 cgccgagcac ccggcgcctg ctgaattcca tgactgctgc gatcccgaaa acgttgtccc
      481 gcagcgcaca gtgcggacgg gaactctaat tttcaagaaa ttgtcggact aatccggcgt
      541 gagccgttag tattttgcga ttacccgagg gcacaggcga aacctggcgc atatcgacaa
      601 gtcgctcaac gccggagtgc taggtcgctc acaaacgggt taaaccgaac cccagcgcta
      661 tggacttggt tccatgcttg cgggatttac tggcattcgt cgggtgagtc gtccggttcg
      721 gctcgtaccc ggctcaagtg ggggaattgg catggtgcgc accgcagcgc gcgtctggcg
      781 ccggggcatt gccggctgtg cgtggttggc cgcggcctcg ggcattgtcg gcgcaggcct
      841 cgccaatgct gctccgcggg acgccttgcg cgcgccgtcc tatgcggaat ccttcaggat
      901 cgggagttcc gggccgctct gcgaagccca ggcggtcgcc gccgggtcgc cgcgcggttc
      961 gatgtttgac cggcgctggg ccttgctctg ccgcgaggtt gcccgccccg tgggtacggc
     1021 ggctttcgtt cgcggcggcg cgcccgccgc ccccggcgat ggcctcgacg aaccgctcga
     1081 ttgcgccgaa ccggcccgtg tcgccgtggc cggcctttcc ggcgtggtcg cccgcgattg
     1141 ccgggggcgg accagcggcg cggcctatcg cagctatgcc ctgcgcaccg ggcgcggcgg
     1201 ctggtcggtg gccggcctcg ccgcctttga cagcgcgctc aagctgaccc tgcgcagcct
     1261 cgtggccgat cgccttgtcc ccggcgcgat cgaggctgtt accctgggca ccggcggggc
     1321 gggcgggttc ttccagtcta aggctgccgc ggccgatgcc gagagcctgc tcgggcaagg
     1381 ctatcgccgc aacgcggccg gcgcctatgc cgaagccgcg cagatcttcg ccgccacggc
     1441 ggccgcaatc ggcgcgggca atgccgattc cgccgcgcgc cgccacgagc tggtaatcaa
     1501 ccaggcgctg cagctctcca atctcggcca gttcgaccag gccgcggtcc tgttcgccca
     1561 ggctcgcgcc atgccgggga tcgatttcgt ccaggcgcgc ctcgcccgca acttcgaggc
     1621 gatcgacgcg ctcaaccggc gcgacgttgc cgccgtcgag gggatcctcg atcgcccggc
     1681 gcccccggtc accgccgggg tgatcgccgg tgatggcgcg gcggagatcg atccggccac
     1741 cgccgcgggc ctcgccaccg gcacagcgcc caatcttgcg cgcattctcg ggcaggacgt
     1801 caggctcacc ccggccgagc gcgcggctat tgccgacgcc caggccctcg ccctgcgcgg
     1861 cacggcgctg cgcctgtcgg gcaagccggc cgaagcccgc accgcgcttg aaggcgccaa
     1921 ccgcgctgcc ctggccgtgc gcgatggccg ggtcgtctcg gtcacccgcc tgcgcagcca
     1981 gatactggcc gagattggcc aggcctggga aagcgaaggc aagcccgccg aggccgaacg
     2041 cctctatcgc gaggtcctcc tcctcgttgc cacccagtat cccgatagcg cgtcggtcaa
     2101 ttccgccaag gcgcgcctcg ccggtttcct ggtccgccag ggccgcatcg atgccggccg
     2161 ggccgactac aaggccctcg tcaccggcgt catcggcgag cgtgacgcgc tggtggggat
     2221 ggccaacctg atccagccct atttcgacct gctcgtcgcc aacggagcca ccagcgaggc
     2281 cgatctcggc gagctgctga tggccagcca gctggtccag cgcccgggcg cggccgatac
     2341 gctgagccag ctgtcccgcc agctcgaagc cgggaatgac gatgccgccg ctctgttccg
     2401 ccgctcgctg gccgtcggcc gcgatatcga gcgcgcccgc gtccagcagg cccgcctggc
     2461 tactgccgag ggcgacaaga ccctgctcgc cgcccaggct gccgatctcg ccgcgcagca
     2521 ggcccggctg gaggaggccc agctccagct ggtcagccag ctctccgcct tcccgcaata
     2581 ccgcgccatc agccgctcct atgtcaccct gcccgaactg cgcgcaacgc tcgggacagg
     2641 ggagggctat ctcaagctcg tgaccttggg cgaacgcacc tatgccttgc tggtcacccc
     2701 cagcggcgga cgcggctggc tggtcggcaa gagcgctgcc gaactggccg acatggtcac
     2761 cgccttgcgc gaatccatct cggtcactgt caacggggtg cgctcgacct atccgttcga
     2821 catcgatacc gatcttgccc tgtcgcaggc cctgctcggc cccgcccggg ccgacctcgc
     2881 cggcattcgc cacctcgtgt tcgagcccga tggggccatg ctccagctgc ccgccaacct
     2941 gctggtgatc gatcccgccg gtgccgatgc ctatcgcgcc cgccttgccg ccgggggtga
     3001 cgaattcgac atgaccggcg ttgcctggct cggccgcacc acggcgatca gcaccgcgct
     3061 ctccgccgcc tccttccggg acagccgcgc cgcgccggcc tcgaaggcgg cccatgccta
     3121 tctcggcctc ggccacaacc agccgctcgg cgcggtaacc accctgccga gcgtgcgcgg
     3181 cgtcgcccag ggcagccctt tcgcgcaagg ctgcgactgg ccggtagcca cctggaacca
     3241 gcccatcgcg gcgaccgagc tgcagcaggc ggccggcctg ttcggccccg ccaacgccgc
     3301 gctgatgacc ggcgcggcct ttaccgatac cgccatcgaa acccggcccg accttgccga
     3361 tttccgcgtg gtccacttcg ccacccacgg tctcgtcacc gcgccgcgca ccggttgccc
     3421 ggcccgcccg gcgctggtta cctcgttcgg cggcaacggc tccgatggcc tcctgcgctt
     3481 cgacgagatc ttcaacctcc atcttgatgc cgatctcgtg atcctctcgg cctgcgatac
     3541 ggccggcggg gccagcctcg atgccacgcg cgaggcgggc gagacttcgg gcggcgggca
     3601 ggcgctcgat ggcctggtcc gcgcgttcat cgctgcgggc gggcggcagg tgatcgccag
     3661 tcactggccc gcgcctgacg atttcaacgc gaccgagcgt ctcgtcggcg gcctcttcgc
     3721 cgccactggc gaggagagcc tggccgaggc cctgcgcctc agcgaagtcg cgctgatgga
     3781 cagtgccgat acgtcgcacc cgttctactg ggccggcttc gcgctgatcg gcgacggccg
     3841 ccgcccgctg cacgcgcgct gatcggacct gcgcgccgtg ggggaccagc cagcacgtct
     3901 gacaccagcg gccgccatca ggcggtcctg gcgtaacgtg cgcggcgccg gggggcgccg
     3961 cctgtcggcc accggggcgg ccctgctgat cgccctgttc ctcgccatct tcgcctggcg
     4021 cgttcccggc ctcggggatg ccgaacggtc gctctatgac ctgcgctcct atctcgccgc
     4081 gcccaaggtg gcgcaggacc cgcgcgtcct gctggtggtc tatgacgatc agaccctgat
     4141 cgccgcccgc aagcgctctc cgctcgatcg cggcctcctc gccaaggcgc tcgccaatct
     4201 tgacgccatg ggcgccaagg ccatcggcat cgacatcctg ttcgaccagc cgcaggacga
     4261 ggacgacgcc ctcgtcgccg ctctgcgcgg aatgaagacg cccaccttcg tcgcctatgc
     4321 gaatgtcgcg ggcaaccgcg acgatattgt ctacgaccag cagcagtatc tcgacgcctt
     4381 ccagcgccgc ctcgtcggct ccaacgcgca ccccgcctcg atccggctgg actttgccca
     4441 aggggcaacc cgccgctggc ccgagatcgt ccccagcctg ccgcctgtcc tcgcccgcgc
     4501 catgctggcg gcggggggtg accagggggc cgtccagcgc ttcgccggct atcgcggccc
     4561 ggtgcgctat cgcctgccgg aaagcgccga tcggcccgtc ttcacctcgc tcaagatcga
     4621 tctcttcgcc gatcccgaga tcgccgccgc catggccgat caggtgcgcg ggcgctacgt
     4681 gatcctcggc ggcgatatcg tcgatgtcga ccgggtgccg accaccttca cctcggtcga
     4741 tggcgaggcc gtgcccggcc ttcaggtcca cgccgcgatg atctcgcaga tgctcgatgg
     4801 cgccgcgcaa ggccgcgtcc ccccactgca acgcgccgca ttcgccgttc tgctggtcgt
     4861 cgccgccacg ctcaccagcc tgctcgaatg gcagccctgg cggctggttc cgttcatcct
     4921 cgtccaggcg gtaggcttcg ggggggtgcc gttcctgctc caggcccgcg gggtcgatac
     4981 ctgggacctg cccgccgccg gctgggctct ggcctggatc atcgcctttg gcgccgtcgc
     5041 ctccgccgcc cgcgcctcta ccgccgcgca gcgccgcttc gctcacgatg cgctgggcaa
     5101 atacctcccg cgcgacatcg cgcgcgagat catcgagaag cccgaactgc tcgccctcca
     5161 tggcgagaag aagcccctgt tcatcgtctt cagcgatctc gagggcttca ccaagctctc
     5221 gcatgatctc ccgccggaaa cggtcgcgcg gcttctaaac cagtatctgg aaatgctgag
     5281 caaggtcgtg ctcgatcacg gcggggtgat cgacaagttc gtcggcgatg ccgtcgtcgc
     5341 cttctggggc gccccggtgg cccggcccaa tgatggcgag cgtgctgcgc gggccgccta
     5401 tgccatgtgg caggcgggcg aggagtttcg ccggatcaat gccgtcgaag gcctgccgcc
     5461 catcggcaag accagggtcg ggctgcacca tggtgaggcc gtcgtcggca atttcggcgg
     5521 cgagcggcgc atccagtaca ccgcgctggg cgatgccatg aacaccgccg cccgcctcga
     5581 aagcgccaac aagccgctcg ccgccagcgt catggccagc cgggaggctg ccgacctgtc
     5641 cgggctcgac tggtggcggc cgatgggcaa ggtggtcctg cgcggccgca cccgcgccgt
     5701 cgaactgcgc gagcccgcgc ccgatttccc ggcggaagac cgagccgccc tggccgaggc
     5761 cctcgccgct ttcgaggcag accgcgccgc cggtcttgcc cagctccgtc tcatcgcagc
     5821 ccggcacccc gccgatgctg ccctcgccaa tctggtaacc cgttgcgaat ccctgggaga
     5881 gcgcgatgcc tatgttctcg attaattacc tggctgccgg cgctgccgcc cttgccctgg
     5941 ccattcccgc agcagccgaa gcggccatcg tcgtcgcctc gtccggcccg tccgctgcca
     6001 cctacccggt cggccgcaag cttgacgatg ccggcacgat caccctgcgc gccggcgata
     6061 cgctgaccgt gctcgacggc aagggcaccc gcgtcctgcg cggcgccggc aacttcccgg
     6121 tcgccgccgc agccggaacc ccggccaaga gcgcgacctt cgccgtgctc accatgcagc
     6181 gctcggccca gcgggtccgc accggggccg tgcgcacggg tgtcggcgcc gaagcgccgg
     6241 ccagcccgaa cctgtggttc atcgatgtcg ccaagcccgg caagcgctgc cttgtcgccg
     6301 gccagccggt tcgcctgtgg cgcctcgaca ccaaggctgc cgccacctac acgctggcgc
     6361 ccgcagctgg cggggcaggc gctgcggtga ccttcgccgc cggctccggc gtcgccccgt
     6421 ggaataccgt ggccatcccg gtcaccgacg gcggccgtta cgtggtgagc gccgggggca
     6481 agcctgtcgg cacggtcgag accgccctgc tgcccgccca gccgcgcgat gccgaagcca
     6541 tggcggcggc gctcatcggc aagggctgca ctgcccaggt cgaactgctc gccgcaacac
     6601 tcgggcagca gtaaatcatg gcgcaccagg gggccttcag gaccatgcga accacaccgg
     6661 ccgtggcaac ggccctcctg cttgcagccc tgccggcggc ggccctcccg gaatccgcca
     6721 gggcacagca agcaccagcc gcacccacgc gcgaggagct gcaggcccag gcccccgccg
     6781 cggaaacccc gcgccagagc cggctgacca tcgag
//
LOCUS       DATNBV010000547         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1886486,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000547 DATNBV010000000
VERSION     DATNBV010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886486"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            549..1850
                     /locus_tag="VN627_12965"
     CDS             549..1850
                     /locus_tag="VN627_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04207.1"
                     /translation="MSTVSIPPILSLLIGHIAAAVPRRARITFTELLIGAAATRGGHV
                     TDAILAAGLSRSWTTYYWLLQRGRWTWLAVWQALLRGLKMLFDPPVWHVVLDDTLVER
                     ISARAPGSLIHHNHTAKPNRPRFLRGQGWLCLAAVVEHEWKVGAVPLMLRLVRRGPNR
                     GKLKSAGFLLRLLGNRLGRVRVLLDAWFMRAWLIERTLAASHTVIGRVRRDLALYEVP
                     KPPGPRGRGRPRKYGARMTRQKIEALPVHRTAQILYGNLEVVRYRTCRVAARFLKGRV
                     VRAVWVQLEQPDRGDQPTEERLLICTDPDLSAITVITSDAKRWSIEALFAAIKHGWGL
                     KDAWQQSRQVLMRWVTILAAGYALNQMLAHTDPARIPGLADPAPWRPVGARTAGLIQA
                     GITRILRVVGLPALMALTSLKSDLQDVHAAPVLASAGPKAG"
BASE COUNT          348 a          697 c          700 g          362 t
ORIGIN      
        1 tgcatctccc aaatgatata cccggtgttg accaatcttt gtgcctgccc agctgtgggc
       61 gactatgcat tccaggccgg gctggttggc tggcgctatc gataccgagc cattgacaca
      121 ggcgacctca aggcaaagct atggctgttt gacggcgcat cgcccgttta cacgggctac
      181 gctggctcga ccttatcgag tgcagtggag tgggggaggt cacagcctac gtcgagccaa
      241 aaccccaagg cttgctgccg gggtattttg cccaacgtgg actctcccac tgaagtttac
      301 agggaggcga tcgggactgc gggcatggcg gggcttgccg gacggggtag gacgggcctt
      361 aaccgtggca cgggatcgca cgagaacccg gcggctgcgg gcaagagggc agcggcggac
      421 aggctttgag gatttcagga tcggtgggca tcgcggggtc cccgggcagg cgagagtgtc
      481 ggcggttttg agcagttgaa ccgacatctg ggcgcgatgc agcgcgtcct ccagggctgg
      541 ttcaaccgat gtccaccgtc tccatcccgc cgatcctgag cttgctcatc ggccacatcg
      601 ccgccgctgt gccgcggcgt gcccgcatca ccttcacgga actgctcatt ggagcagcgg
      661 cgacccgagg cggccacgtc acggacgcga tcctggcggc cgggctgtcg cgcagctgga
      721 ccacgtacta ctggctgctt cagcgcgggc gctggacatg gctcgccgtc tggcaggccc
      781 tgctgagggg gctcaagatg ctgtttgacc cgccggtctg gcacgtggtc ctcgatgaca
      841 cgctggtcga gcggatttcc gcccgggcgc cggggtcgct gatccatcac aaccatacgg
      901 ctaagccgaa ccggccgcgg ttcctgcgtg gccaaggctg gctgtgcctg gccgcggtcg
      961 tcgaacatga gtggaaggtc ggagcggtgc cgctgatgct gcgcttggtc cgccgtggcc
     1021 ccaatcgcgg caagctcaag agcgccggct tcctgctgcg cctgcttggc aaccggctcg
     1081 gccgggtgcg cgtcctgctc gatgcctggt tcatgcgcgc ttggctgatc gaacgcaccc
     1141 tcgcggccag ccacaccgtg atcggccggg tccgccggga cctcgcgctc tatgaggttc
     1201 caaagccgcc tgggccgcgg ggtcgcggac ggccccgcaa gtacggcgcg cggatgacgc
     1261 gccaaaagat cgaggcgctg ccggtccacc gcacggccca gatcctgtac ggcaatctgg
     1321 aagtcgtgcg ctaccgaacc tgtcgcgtcg ctgcgcgctt cctgaaaggg cgggtggtcc
     1381 gcgccgtatg ggtgcagttg gagcagcccg accgcggcga tcagccgacc gaggagcgcc
     1441 tgctgatctg caccgacccc gacctgtctg cgatcacggt catcaccagc gacgccaaga
     1501 ggtggagcat cgaagcgctg ttcgcagcca tcaagcacgg ctgggggctc aaggatgcct
     1561 ggcagcagtc gcgccaagtg ttgatgcgct gggtgaccat tctggccgcg ggctacgcgc
     1621 tcaatcagat gctggcccac accgacccgg cccgcatccc gggtctcgcc gacccggcac
     1681 cctggcggcc agtcggggcg cgaacggccg ggctgatcca ggcaggcatc acgcggattt
     1741 tacgcgtggt cggcctgccg gccctcatgg ccctgacctc gttaaaatca gacctccagg
     1801 acgttcacgc cgcccccgtg ctggcgtccg ccggccccaa agccggctga gccaacactc
     1861 cagacgtcct cacgccgacc acgcggccgc agttcgccgc agggggcaaa tccctctgcc
     1921 tgtaaacttc aggatattaa tccacatgcc gccgccgctg cagcagagaa tgcgcgagct
     1981 aacggactcg gtgagcgcgt catcgcggtc ggaatgaacc tgctttcagg gtttcagccc
     2041 agcccgatgt tcgatgttgt gatttccagc ccgcccaagc acgcaggcga accaagggac
     2101 ctagctg
//
LOCUS       DATNBV010000548         6353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4420506,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000548 DATNBV010000000
VERSION     DATNBV010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4420506"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VN627_12970"
     CDS             <1..384
                     /locus_tag="VN627_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HXD04208.1"
                     /translation="RVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDIQGEFAQC
                     DVAFVIGTNDVVNPAAKTDKSSPIYGMPVFDVEKAKTIMFIKRSMGGVGYAGVDNDVF
                     YMDQTMMLLADAKKMVEDIVKVLAH"
     gene            complement(393..632)
                     /locus_tag="VN627_12975"
     CDS             complement(393..632)
                     /locus_tag="VN627_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04209.1"
                     /translation="MQALKSFRLAAAAAALLPLAALAPATAEAKGCLRGAAAGAVAGH
                     MAHHHAIAGAMIGCAAVHHHYAKKARAQAVPAHRT"
     gene            692..1018
                     /locus_tag="VN627_12980"
     CDS             692..1018
                     /locus_tag="VN627_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04210.1"
                     /translation="MNSPSRSAPILLGALAVAALALSGCVERIAESRVRSALVDAGLS
                     DQTSACMARRMVDRLTISQLRKLQALRGPKRSLADYVLAVRKVGDREVLEVTASSAAL
                     CATGFG"
     gene            1105..1797
                     /locus_tag="VN627_12985"
     CDS             1105..1797
                     /locus_tag="VN627_12985"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HXD04211.1"
                     /translation="MRKLGLIGGMSWLSTRTYYDHLNRLVQASRGRQASAPLLIESLD
                     YTDLQRLQSEEEWDRAAQILADSARRLEQAGATAVIIGSNAMHKVYDAVAGAVGVPVL
                     HIAEAVAERMARDKVRKAALIGTRNVMAETFYRQKLIARDIELLPPQMRFVETLDRII
                     YDELMLGKATRQSERELKTMMVELDRAGAQAVVLGCTELEMVVDVDANVLPIYDCTRI
                     HAERAAEWILGA"
     gene            1797..2963
                     /locus_tag="VN627_12990"
     CDS             1797..2963
                     /locus_tag="VN627_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HXD04212.1"
                     /translation="MASSRATYACDPARSRGREFAADDTVPRGPRTCYQRDRDRIIHS
                     IAFRRLRHKTQVFVAPDGDHYRVRLTHSLEVAQIGRVIARTLGLDEDLTEALCLAHDI
                     GHPPFGHAGEEALNAALAPHGGWDHNAHCLRTLMRLETPYCEFDGLNLTWELLEGLAK
                     HNGPVTAPNWALAELDRAWPLDLGEWSCLEAQVASLADDIAYDNHDIDDGLRAGFLEL
                     DDLLTLDFVGEQWRAVERRFPRAERDRQLRELVRGQIGLMVNDLLGETRQRTAGMGGP
                     ADIRAAGRATAAFSPELAANERRLKAFMYERLYLHPEQLATARKAGEVIATLFAAYSA
                     EPDLMGPGWAERLREAEPDRSRHIADYLAGMTDRFAIAAYARITGITPEGLRNV"
     gene            2956..3669
                     /locus_tag="VN627_12995"
     CDS             2956..3669
                     /locus_tag="VN627_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXD04213.1"
                     /translation="MSERVRICLLGASGLVGSALIRACVGRDDVRLVAVARSELALPP
                     GARMEVLLADPANWGDAIAAARPQVLVSALGTTWARSGKDEAAFRAVDQELVLACARW
                     GVEAGARAMIAISSVGADPHARGLYLRVKGEVDQALNKVGYARLDIIRPGLLRGRRSE
                     RRLLEGLGQIVAPLVDPLLGGNLARYRSIRAATLAEAILALAHEKARGRFVHEWPSLE
                     RALKRQAFQASLRQQVAVE"
     regulatory      3733..3854
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      3856..3994
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            4089..5189
                     /gene="gcvT"
                     /locus_tag="VN627_13000"
     CDS             4089..5189
                     /gene="gcvT"
                     /locus_tag="VN627_13000"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HXD04214.1"
                     /translation="MLPLDSWHRARGGRMVEFAGYWMPVQYEGIIAEHLWTRDHAGLF
                     DVSHMGQLLVSGEGAAGALEALLPGDISALNPGRQRYSLLLDETGGILDDLMVTNRGD
                     HFYLVVNGATKWDDIAHLREYLPDEITLNLLDEHGLLALQGPDAAASLGALGLHPAFD
                     GMPEVVHLKFMQAAAYHWGEVPLGVSRSGYTGEDGYEISVRAADIEALAEALCALQQV
                     KPIGLGARDSLRLEAGLPLYGHDLSPETDPVSAGLAFAISKRRRTEGGFLGAEPVLAA
                     LEGGTATRRVGLALEGRQAAREGALVFAGEEEVGRVTSGGFSPSLNHPIAMAYVNAAH
                     AADGTALTIEVRGKRLPARVVPMPFVPHRYHR"
     gene            5201..5572
                     /gene="gcvH"
                     /locus_tag="VN627_13005"
     CDS             5201..5572
                     /gene="gcvH"
                     /locus_tag="VN627_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HXD04215.1"
                     /translation="MTTYYTKDHEWIAVEGETGTVGITDYAQGQLGDITFVELPEPGS
                     AVKQGDAPCVVDSVKAASDVYAPVGGTVTEANAALADAPELVNTEAESGGWLFRLTLA
                     DPGQLGGLMDRPAYDEYVKGL"
     gene            5700..>6353
                     /locus_tag="VN627_13010"
     CDS             5700..>6353
                     /locus_tag="VN627_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HXD04216.1"
                     /translation="MRYLPLTPAERADMLRTIGAASIDELFVDVPPEARLDGPIAGLP
                     GHASEMAVERHMARLAADNLSAGSAPFFLGAGAYRHHIPASVDHLIQRGEFLTAYTPY
                     QPEIAQGTLQVLFEFQTQVARLFGTDIANASMYDGSTACWEAVAMAGRITRRKKAILS
                     GGLHPHYVSTATTMARFTGDALDTRLPELSAEPDDAALIAAIDGETSCVVVQYPDVLG
                     "
BASE COUNT         1007 a         2325 c         2029 g          992 t
ORIGIN      
        1 cgggtgaagt acgcgatcca tcccgtcgcc ggacggatgc cggggcatat gaacgtgctg
       61 ctcgccgagg cgaacgtgcc gtacgatgag gtgttcgagc tggaggacat ccagggcgaa
      121 ttcgcccagt gcgacgtcgc cttcgtcatc ggcaccaacg acgtggtcaa ccccgccgcc
      181 aagacggaca agtcatcgcc gatctacggc atgccggtct tcgacgttga gaaggccaag
      241 accatcatgt tcatcaagcg ctcgatgggc ggcgtcggct atgcgggcgt cgacaacgac
      301 gtgttctaca tggaccagac catgatgctc ctggccgacg ccaagaagat ggtcgaggac
      361 atcgtcaagg tgctggccca ctagtctccc gctcaggtcc ggtgcgccgg caccgcttgg
      421 gcgcgcgcct tcttggcata gtgatggtgc acggccgcgc agccgatcat cgcgccggcg
      481 atggcgtgat ggtgcgccat gtgcccggcc acggcgcccg ctgccgcccc gcgcaggcag
      541 cccttggctt cggcggtggc gggggcgagg gccgccagcg gcagcagggc ggcagcggcg
      601 gcggcaagac gaaacgattt gagggcttgc atcggcttcg aactcctgtt gaaccaatgc
      661 catgccaatc ccccgcgctt cagcccgttt catgaattcg ccgtcacgtt cggcaccgat
      721 tctcctcggc gccctcgcgg tcgccgccct cgcgctgagc ggctgtgtcg agcggattgc
      781 cgaaagccgc gtgcgctcgg ctctcgtcga tgccggcctg tcggaccaga ccagcgcctg
      841 catggctcgg cggatggttg accggctgac catttcccag ctgcgcaagc tccaggccct
      901 gcgcggcccc aagcgctcgc tggcggacta tgtcctggcc gtgcgcaagg tgggggaccg
      961 cgaagtgctc gaagtcaccg cctcctccgc cgccctgtgc gccaccgggt tcggctgacc
     1021 gcacgcagcc gctggaccga atccccgcgc ccacctaagg gcagggcata gggggtcctt
     1081 cacacttccc gggagatact gaccttgcgc aagctcggcc ttatcggcgg catgtcctgg
     1141 ctgtcgacac gcacctacta cgatcacctc aaccggctcg tccaggcgag ccggggtcgt
     1201 caggccagcg cgcccctgct gatcgagagc ctcgattata ccgacctcca gcgcctgcag
     1261 agcgaggagg agtgggacag ggcagcgcag atcctggcag acagcgcccg ccgtctcgaa
     1321 caggccggcg ccaccgccgt gatcatcggc tccaatgcca tgcacaaggt ctacgatgcc
     1381 gtcgccggcg ccgtcggcgt gcccgtgctc cacatagccg aggccgtggc cgaacgcatg
     1441 gcgcgtgaca aggtgcgcaa ggccgccctg atcggcacgc gcaacgtcat ggccgaaacg
     1501 ttctatcgcc agaagctcat cgcccgcgac atcgagctgc tcccgcccca gatgcgcttc
     1561 gtcgaaacgc tcgaccggat catttacgat gaactcatgc tcggcaaggc cacccgccag
     1621 tccgagcgcg agctgaagac gatgatggtc gagcttgacc gcgccggcgc ccaggccgtc
     1681 gtcctcggct gcaccgagct ggaaatggtg gtcgatgtcg atgccaatgt cctgccgatc
     1741 tacgattgca cccgcattca tgccgaacgc gcggcggagt ggatcctcgg cgcctgatgg
     1801 cctcttcgcg tgccacctat gcctgcgatc ccgcacgcag ccgggggcgc gaattcgcgg
     1861 cggacgacac tgtcccgcgc gggccgcgca cctgctacca gcgtgatcgc gaccggatca
     1921 tccattccat cgccttccgc cgcctgcgcc acaagaccca ggtcttcgta gcgcccgatg
     1981 gcgatcacta ccgcgtccgc ctcacccaca gcctcgaggt tgcccagatc ggccgggtca
     2041 tcgccagaac cctcgggctc gacgaggacc tgaccgaggc cctgtgcctc gcccacgata
     2101 ttggccaccc acccttcggc catgccggcg aggaggcgct gaacgccgcg ctcgctcccc
     2161 acggcgggtg ggaccacaac gcccactgcc tgcgcaccct gatgcgcctc gaaaccccct
     2221 attgcgagtt cgacgggctc aacctcacct gggaactgct cgaaggcctc gccaagcaca
     2281 acggcccggt aaccgcgccc aactgggcgc tcgccgaact cgaccgcgcc tggccgctcg
     2341 atcttggcga atggtcctgc ctcgaagccc aggtcgcttc gctggccgac gacatcgcct
     2401 atgacaacca cgatatcgac gatggcctgc gcgccggctt cctcgaactg gacgatctgc
     2461 tgaccctcga tttcgtgggc gaacaatggc gcgcggtcga gcgccggttc ccccgggcgg
     2521 agcgcgatcg ccagctgcgc gaactggtgc gagggcagat cggcctgatg gtcaacgatc
     2581 tgctcggcga aacccgccag cgcaccgccg gcatgggcgg ccctgccgat atccgcgccg
     2641 ccgggcgcgc caccgccgcc ttctcccccg aactcgccgc caacgaacgc cgcctcaagg
     2701 ccttcatgta cgaacggctc tatctccacc ccgaacagct cgccaccgcc cggaaggcgg
     2761 gcgaggtgat cgccaccctc ttcgccgcct attccgccga gcccgatctg atgggtcccg
     2821 gctgggccga acgcctgcgc gaggccgagc ctgaccgcag ccgccacatt gccgactacc
     2881 tcgccgggat gactgaccgt ttcgccatcg ccgcctacgc ccggatcacc gggatcacgc
     2941 cggaaggcct caggaatgtc tgagcgggtc cgcatctgcc tgctcggcgc cagcggcctc
     3001 gtcggctcgg ccctgattcg cgcctgcgtc gggcgcgacg atgtccggct cgtcgccgtc
     3061 gcccgcagcg aactcgccct gccgcccgga gcgcgaatgg aggtcctgct ggccgacccc
     3121 gccaactggg gcgatgccat cgccgcggcc cgcccgcagg tgctggtctc cgcgctcggc
     3181 accacctggg ccaggtccgg caaggacgag gcagcgtttc gtgccgtcga tcaggagctg
     3241 gtcctcgcct gcgcccgctg gggtgtcgaa gccggcgcgc gggcgatgat cgcgatcagc
     3301 tcggtcggcg ccgatcccca cgcccggggc ctctatcttc gggtcaaggg cgaggtcgac
     3361 caggccctta acaaggtcgg ctatgcccgg ctcgacatca tccgtcccgg cctgctgcgc
     3421 gggcggcgga gcgaacggcg cctgctcgaa gggctggggc agatcgtcgc gccgctggtc
     3481 gatccgctgc tcggcggcaa tctcgcgcgc tatcgctcga tccgggctgc caccctggcc
     3541 gaggcgatcc tggccctcgc ccacgaaaag gcgcgcggtc ggttcgtcca cgaatggccc
     3601 tcgctggaac gcgcgctgaa gcggcaggcc ttccaggcct cgctgcgcca gcaggtggct
     3661 gtggaatagt ccgaggcatc gcgggcactt gcgattgact tcgcaagtgc gaacagccag
     3721 caccaaggcg acgctgaccg cgcgggagag tccgttgcga atcgcggccc ttccattcgg
     3781 ggcccgccat cgccacggcg ccgaaggagc aaccgccccg gaatctctca ggcatcaggg
     3841 accgcgtggg gtcgatagcg tcgctctgga aagtgccttg tccggcccgg gattcgggaa
     3901 gggcagggcc gccgaagggg taacccggtt gtctccgccc cactggcggg ggaacggggg
     3961 aaagctctca ggcttccgtg acagaggggg cacgtgaacc gtgtgccaat cctgtgcgga
     4021 gcatgctcgt gcctgatgac gatgcctcga tcgaggccaa tgacaatctt gatgaacttg
     4081 agccggagat gctcccgctc gattcctggc atcgcgcgcg cggcggccgc atggtcgaat
     4141 tcgccggcta ctggatgccg gtccagtatg aaggcatcat cgccgaacac ctgtggacgc
     4201 gcgatcacgc cggcctgttc gacgtctcgc acatgggcca gctgctggtt tcgggggagg
     4261 gcgcggccgg tgcgctcgaa gccctcctgc cgggcgacat ctccgcgctg aaccccggcc
     4321 gccagcgcta ttcgctcctg ctggatgaga ccggcggcat cctcgacgat cttatggtca
     4381 ccaaccgcgg cgatcacttc tacctcgtcg tcaatggcgc caccaagtgg gacgacatcg
     4441 cccacctgcg cgaatacctg cccgacgaga tcaccctcaa tcttctcgac gaacacggcc
     4501 tgctcgccct gcaggggccc gacgcggccg ctagcctcgg tgcgctgggc ctgcaccccg
     4561 ccttcgacgg catgcccgag gtcgtccacc tcaagttcat gcaggccgcc gcctatcact
     4621 ggggcgaggt tccgctcggc gtcagccggt cgggctatac cggcgaggac ggttatgaga
     4681 tctcggtgcg cgcagccgat atcgaagccc tggccgaagc cctctgcgcc ctgcagcagg
     4741 tcaagccgat cggcctcggc gcgcgcgaca gcctgcggct tgaagccggc ctgccgctct
     4801 atggccacga cctttcgccc gagaccgatc cggtcagcgc cggcctagcc ttcgccatca
     4861 gcaagcgccg ccgcaccgaa ggcggcttcc tcggtgccga gccggtgctg gccgcccttg
     4921 agggtggcac cgccacccgg cgcgtcggcc tcgccctcga aggccgccag gccgcccgcg
     4981 aaggcgcgct ggtttttgcc ggcgaagagg aggtcggcag ggtcacctcc ggcggtttct
     5041 cgcccagcct caatcacccc atcgccatgg cctatgtcaa cgccgcccat gccgccgatg
     5101 gcacggcgct caccatcgag gtgcgcggca agcggctgcc ggcgcgcgtg gtgcccatgc
     5161 ccttcgttcc ccaccgctat caccgctgag gagatccgcc atgaccacct attacaccaa
     5221 ggaccacgag tggatcgccg ttgaaggtga gaccggcacc gtcggcatca ccgactatgc
     5281 ccagggccag ctgggcgaca tcaccttcgt cgaactgccc gagcccggca gcgccgtgaa
     5341 gcagggcgat gccccctgcg tggtcgacag cgtcaaggcc gcctccgatg tctatgcgcc
     5401 tgtcggcggc acggtcaccg aggccaacgc ggcgctcgcc gacgcgccgg aactggtcaa
     5461 caccgaggcc gaaagcggcg gctggctgtt ccgcctgacc cttgccgatc ccggccagct
     5521 cggcggcctg atggatcggc ccgcctacga cgaatacgtg aagggcctct gagcctgcaa
     5581 tcctccccgc tggcggggag gggaaccgcc ggaacggcgg aggagggggc tctcctgcaa
     5641 acggcagccc tcgccggcac ccgccctccc cacgggtggg aggatcaagg agcgaaccaa
     5701 tgcgatacct ccctctgacc ccggctgaac gcgcggacat gctccgcacg atcggcgcgg
     5761 ccagtatcga tgagctgttt gtcgacgttc cgccggaggc ccggctcgac ggcccgattg
     5821 ccgggctccc gggccacgcc agcgaaatgg cggtggagcg gcacatggcg cggctcgcgg
     5881 ctgataatct ctcggccggc agcgcgccgt tcttcctcgg ggcaggggcc tatcgccacc
     5941 acatcccggc ctcggtcgat cacctgatcc agcgcggcga attcctcacc gcctataccc
     6001 cctaccagcc ggagatcgcc cagggcaccc tgcaggtcct gttcgaattc cagacccagg
     6061 tcgcccgcct cttcggcacc gatatcgcca atgcctcgat gtacgacggc tcgaccgcct
     6121 gctgggaggc cgtggccatg gccgggcgca taacccggcg caagaaggcc atcctctcgg
     6181 gcggcctcca tccccactat gtctcgaccg cgacgaccat ggcccgcttc accggcgacg
     6241 ctctggacac ccgcctcccc gagctctccg ctgagcccga tgacgccgcc ctgatcgcgg
     6301 ccatcgatgg cgagacctcc tgcgtcgtcg tccagtaccc ggacgtgctc ggc
//
LOCUS       DATNBV010000549         5981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1887839,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000549 DATNBV010000000
VERSION     DATNBV010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887839"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VN627_13015"
     CDS             <1..217
                     /locus_tag="VN627_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04217.1"
                     /translation="IPGAGQHVGSIAGTHPTARVFGPAIRSVNRCLVSLFFSQLTIFT
                     RRYGGASASQLLRVETMLPNVRASSRA"
     gene            252..1706
                     /locus_tag="VN627_13020"
     CDS             252..1706
                     /locus_tag="VN627_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04218.1"
                     /translation="METITIDGVDEHAFRHSVEDMLRYDNVDEATEKLRALLEPYAGP
                     GGPLPARFLAVTAADVEITGWDRLASRLGSYDRPQFPISAIGVVLSDARVLGGPGPKD
                     GRLAPFIKTYYFSDDAYPFTNAARDDLLDGYSRDGFGWQGDYQATDATLAIKGIDDLH
                     GAIIGLEDRLLDSANPPEDQLRAGTIGACFLAALIHQALRDTIRQQGLPRPLCVLAAC
                     DGVYPFFDAPVAGWDDCLTAAAAEAATGEAWPAELEADEAALEGEGSLLSIVSRKGTK
                     HLALELGEDDLREAARLAQDAGAQRLVIGDEALKGLFHGVPMAELVPEEDATASLAEA
                     GEEPQALTQMPVPPTEIELAPEPAPEPEPEPEPFDYYAQARAAFRQQEEAQAFPTGRS
                     LRNRFKEEPVVRESPLAARAKALRRQWRRQARRTRVQLARKLIAGRCKAAAAAEQIGH
                     AGARLNPAPALAALRRAGARWQARLSAMVPRRRR"
     gene            complement(1726..2508)
                     /locus_tag="VN627_13025"
     CDS             complement(1726..2508)
                     /locus_tag="VN627_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXD04219.1"
                     /translation="MTYETILVEQQGAVTLITINRPQALNALSPQVLDEAIRAFAAFE
                     EDPGQRCAVVTGAGDKAFVAGADIKIMADMAATDFYAQDFFGDWTSKLLKATRKPWIA
                     AVNGFALGGGCELAMMADFIIASDTAKFGQPEIKLGVGPGMGGSQRLTRAVGKAKAME
                     MCLTGRMMDAVEAERSGLVSRVVPAAELLADALKTAQAIAAMPPLAAIANKEMVNAAF
                     ELTLDQGLVFERRLFQILAATEDKTEGMQAFIEKRPGVWKGR"
     gene            complement(2525..3397)
                     /locus_tag="VN627_13030"
     CDS             complement(2525..3397)
                     /locus_tag="VN627_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXD04220.1"
                     /translation="MNTVGIVGAGQMGIGIAQIAAQAGMRVLLSDVSQELAEKGAGRI
                     AGGLDRMVAKGELDQAARDKAVGLIVPLAGVDSMAEAALVIEAATEREEIKRAIFANV
                     GKVLGPDAILATNTSSISVTRLAQAVAAPERFIGMHFFNPVPRMPLLEIIRGMASSEA
                     TVAAAKAFGEATGKVAIVATDRPGFVVNRILLPLLNEAYFAVEERLASIPDIDAGVKL
                     GLGHPMGPLTLSDMIGLDTILEILQVFQADFGDPKYRPAPILRKLVEAGWLGRKTGKG
                     FYDYSGETPVPIAL"
     gene            complement(3424..4923)
                     /locus_tag="VN627_13035"
     CDS             complement(3424..4923)
                     /locus_tag="VN627_13035"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HXD04221.1"
                     /translation="MRLIDHFLDGGTAGLRADRKADVFDPNSGAVQAQVVLGGQDALD
                     RAVAAAQAAQPGWAGTNPQRRARVMFRFKELVEANMDELARLLSSEHGKVIADSKGDF
                     QRGLEVVEFACGVPHALKGEYTQGAGPGIDVYSMRQPIGIGAGITPFNFPAMIPLWMC
                     AVAIACGNAFICKPSERDPSVPVRLGELFLEAGLPAGIFQVVHGDKEMVDAILDHPAI
                     AAVSFVGSSDIAHYVYNRGVAAGKRIQAMGGAKNHGIVMPDADLDQVVNDLCGAAFGS
                     AGERCMALPVVVPVGADTAERLKAKLIPAIEALRVGVSTDAEAHFGPVVTAEHKAKIE
                     QWIQTCADEGGEIVVDGRGCTLQGHENGFFVGPTLIDNVTPEMDSYHNEIFGPVLQMV
                     RAANFEEALALPSKHQYGNGVALFTRNGHAAREFAQRVNVGMVGINVPIPVPVAYHTF
                     GGWKRSAFGDTNQHGMEGIRFWTKVKTVTQRWPDGSPDGGNAFVIPTMA"
     gene            5010..5909
                     /locus_tag="VN627_13040"
     CDS             5010..5909
                     /locus_tag="VN627_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXD04222.1"
                     /translation="MQKPDWNDLRIFLAVARTGQLARAGALLGVNGTTVARRVRRLES
                     QLGGILFEQTREGQVLTEAGELLLLHVEAMAGAAGEIETVGGPVTSALTGTLRVSVAE
                     GFGSWFLARHVPEFVAANPNLTLDLVANSGFLSVTKREADIAVMLSRPRTGALISGKL
                     SDYTLQLFATREYLACRGIPRSSSDLADGHRFIGYIPDLIYAPELKYLDEVQPGLRPE
                     IRSSSINAQYHLVASGSGIAVLPSFIGKVDPQLQVLLPDFRIVRSFWLVTHKDTHQLA
                     RVRAGREWIVDMARRHRSALVAE"
BASE COUNT          986 a         2046 c         1939 g         1010 t
ORIGIN      
        1 tattcctgga gccggtcagc atgtcggctc cattgccgga acccacccga ctgcacgcgt
       61 cttcggaccg gcaatccgca gcgtgaatcg ttgcttggtt tcattatttt tcagccaatt
      121 aaccatcttc acccgccgct acgggggcgc cagcgcttct caactgctcc gcgtcgaaac
      181 catgttgcct aacgtgcggg caagcagtcg cgcttagtct gtattgacca gattctcgcg
      241 ggggccttcc gatggagacc atcaccatcg atggcgtgga cgaacacgcg ttccgccact
      301 cggtcgaaga catgttgcgc tacgacaacg tcgatgaggc gacggagaag ctgcgcgcct
      361 tgctggagcc ctatgccggg cccggggggc cgctgcctgc ccgcttcctc gccgtgaccg
      421 ccgccgacgt ggagatcacc ggatgggacc gcttggccag ccgcctcggc agctacgacc
      481 ggcctcaatt tccgatctcg gccatcggcg tcgtcctctc cgacgcccgg gtgctcggcg
      541 gccccggccc caaggatggc cggctcgctc ccttcatcaa gacctactac ttcagcgacg
      601 acgcctaccc cttcaccaat gccgcccgcg acgacctcct cgacggctac tcgcgcgacg
      661 ggttcggctg gcagggcgat taccaggcga ccgatgccac cctggcgatc aaggggatcg
      721 acgatctcca cggcgcgatc atcgggcttg aggaccgtct gctcgacagc gccaatcccc
      781 ccgaggacca gctgcgcgcc ggaaccatcg gcgcgtgctt cctcgccgcc ctgatccatc
      841 aggccctgcg cgacaccatt cgtcagcagg gcctgccccg cccgctctgc gtgctggcgg
      901 cttgcgacgg cgtctacccg ttcttcgatg cgcccgtggc cgggtgggac gattgcctga
      961 ccgccgccgc cgccgaagcc gcgacgggcg aggcctggcc cgccgagctt gaggccgacg
     1021 aggccgcgct ggagggcgaa ggcagcctgc tcagcatcgt ctcgcgcaag ggcaccaagc
     1081 acctcgccct tgaactcggc gaggacgacc tgcgcgaggc ggcgaggctc gcccaggacg
     1141 ccggcgccca gcgcctggtc atcggggacg aggcgctcaa gggcctgttc cacggcgtgc
     1201 ccatggccga actggtccca gaggaggacg cgaccgccag cttggccgag gcaggcgagg
     1261 agcctcaggc actgacgcaa atgccggtcc ctccgaccga aatcgagctt gctcccgaac
     1321 cggcccccga accggagccg gaacccgaac cgttcgacta ctatgcccag gcccgcgcgg
     1381 ccttccggca gcaggaagaa gcccaggcct ttcctaccgg cagaagcctg cgcaaccggt
     1441 tcaaggaaga accggtggta cgcgagagtc cgctggcggc acgggcgaag gcgctccgcc
     1501 ggcaatggcg gcgtcaggct cgccgcaccc gcgtgcaact ggcccgcaag ctcattgccg
     1561 gtcgatgcaa ggccgccgcg gcagcggagc agatcgggca tgccggtgcc cggctgaacc
     1621 cggcgccggc gctggccgcc ctgaggaggg cgggcgcccg ctggcaggcg cggctatcgg
     1681 ccatggtgcc gcgccgccgc cgataggcag ccggtgaggg gccggttacc ggcccttcca
     1741 cacgcccggg cgcttctcga tgaaggcttg cattccctcg gtcttgtctt ccgttgcggc
     1801 caggatctgg aacagccgcc gctcgaacac gaggccctga tccagcgtca gctcgaacgc
     1861 ggcgttgacc atttccttgt tggcgatggc ggccagcggc ggcatggcgg caatggcctg
     1921 ggcagtcttc agcgcgtcgg cgaggagctc ggcggccggg accacgcggc tgacgaggcc
     1981 gctgcgctct gcctcgaccg cgtccatcat ccgcccggtc aggcacattt ccatggcctt
     2041 ggccttgccc accgcgcggg tcaggcgctg cgagccaccc atgccgggcc cgacgcccag
     2101 cttgatttcg ggctggccga acttcgccgt gtcggaggcg atgatgaaat cggccatcat
     2161 cgccagctcg cacccgccgc cgagcgcgaa gccgttaacc gcggcgatcc atggcttgcg
     2221 ggtggccttc agcagcttgc tggtccaatc gccgaagaaa tcctgcgcgt agaaatcggt
     2281 cgcggccatg tcggccatga tcttgatgtc ggcgccggcc acgaaggcct tgtcgcctgc
     2341 cccggtcacc acggcgcagc gttggcccgg atcctcctcg aacgcggcga aggccctgat
     2401 cgcctcatcc agcacctgcg ggctgagcgc gttcagcgcc tgggggcggt tgatggtgat
     2461 gagggtgacg gcaccctgct gttcgacgag aatggtttcg taagtcatgg ccgcaaggcc
     2521 tccctcagag cgcgatcggg accggagtct cacccgaata gtcgtagaac cccttgccgg
     2581 tcttgcggcc gagccaaccg gcttccacca gcttgcgcag gatcggcgcc gggcggtact
     2641 tggggtcgcc gaagtcagcc tggaagacct ggaggatttc caggatcgta tcgaggccga
     2701 tcatgtcgga cagggtcagc gggcccatcg gatggcccaa tcccagcttc acgccggcat
     2761 cgatatcggg gatggaagcg agccgctcct cgaccgcgaa gtaggcttcg ttgagcagcg
     2821 gcaggaggat gcggttgacg acgaagccgg ggcgatcggt ggccacgatc gcgaccttgc
     2881 cggtcgcctc gccgaaggcc ttcgccgcgg cgaccgtcgc ctcgctgctc gccatgccgc
     2941 ggatgatctc gagcaggggc atgcgcggga cgggattgaa gaagtgcatg ccgatgaacc
     3001 gctcgggcgc ggccacggcc tgggcgaggc gggtcaccga gatcgacgag gtgttggtgg
     3061 caaggatcgc gtccggcccc agcaccttgc cgacattggc gaagatcgcc cgcttgatct
     3121 cctcgcgctc ggtcgccgcc tcgatgacga gggcggcctc ggccatggag tcaacccccg
     3181 ccaggggaac gatcagcccc acggccttgt cccgcgcggc ctggtcgagc tcgcccttgg
     3241 cgaccatgcg gtcgagcccg ccggcaatgc ggccggcgcc cttctcggcc aattcctgcg
     3301 aaacatccga cagcagcacc cgcatgcccg cctgggccgc gatctgggca atgccgatgc
     3361 ccatctggcc ggcgccaacg atccctaccg tgttcatcgc aattcccccc tggtttcgtg
     3421 caatcaggcc atggtcggga tgacgaaggc attgccgcca tcgggcgaac catccggcca
     3481 gcgctgggtc acggtcttga ccttggtcca gaaccggatg ccttccatgc cgtgctggtt
     3541 ggtatcgccg aaggccgagc gcttccagcc gccgaaggtg tggtaggcca ccggcacggg
     3601 gatcggcacg ttgatgccga ccatgcccac gttcacgcgc tgggcgaact cacgcgcggc
     3661 atggccgttg cgggtgaaca gggccacgcc attgccgtac tggtgcttgc tgggcagcgc
     3721 cagcgcctcc tcgaaattgg cggcgcggac catctgcagc acggggccga agatctcgtt
     3781 gtggtaggag tccatctccg gcgtgacgtt gtcgatcagc gtcgggccga cgaagaaccc
     3841 gttctcatga ccctgcaagg tgcagccgcg tccgtcgacc acgatttccc cgccctcgtc
     3901 ggcgcaggtc tggatccatt gctcgatctt ggccttgtgc tcggcggtga ccacggggcc
     3961 gaaatgggcc tcggcatcgg tcgacacgcc cacgcgcagg gcttcgatcg ccgggatcag
     4021 cttggccttc agccgctcgg ccgtatcggc gcccaccggc accaccaccg gcagcgccat
     4081 gcagcgttcg cccgccgagc cgaatgccgc gccgcacagg tcgttcacca cctggtcgag
     4141 atcggcatcg ggcatgacga tgccgtggtt cttggccccg cccattgcct ggatgcgctt
     4201 tccggcggcc accccgcgat tgtagacata atgggcaatg tccgacgagc cgacgaagct
     4261 gaccgcggcg atcgccggat ggtcgaggat cgcgtcgacc atttccttgt cgccgtgaac
     4321 cacctggaag atgcccgccg gcagcccggc ctcgaggaac aactcgccca gtcgcaccgg
     4381 cacggaagga tcgcgctccg aaggcttgca aatgaaggca ttgccgcagg cgatggcgac
     4441 ggcacacatc cacagcggga tcatcgccgg gaagttgaac ggagtgatcc ccgccccgat
     4501 gccgatcggc tggcgcatcg aatagacgtc gatccccggg ccggcgccct gggtatattc
     4561 ccccttgagc gcgtgcggca cgccgcaggc gaactccacc acctcgagcc cgcgctggaa
     4621 atcgcccttc gaatccgcga tgaccttgcc atgttcggag ctgagcaggc gggccagctc
     4681 gtccatgttg gcctcgacca gttccttgaa gcggaacatg acgcgggccc ggcgctgcgg
     4741 gttggtcccc gcccagcccg gctgcgccgc ctgggcggcc gcgaccgcgc ggtcgagcgc
     4801 gtcttgccca cccagcacga cctgtgcctg caccgccccg ctgttgggat cgaatacatc
     4861 ggccttgcga tcagcccgga ggccggcggt cccgccatcg aggaaatggt cgatcaggcg
     4921 catcactagg gatcccaaaa atctacacaa ctgatgccct ccgacatttt cgcggacaac
     4981 ccgcaatttt gcgcttaagg attgcaatca tgcagaagcc ggattggaat gacctgcgga
     5041 tttttctcgc ggtagcgcgc accgggcagc tcgcccgggc gggtgcgctc ttgggtgtga
     5101 acggaacaac ggtcgcccgc agagtccggc gacttgaaag ccaattaggg gggattcttt
     5161 ttgaacaaac ccgcgaaggc caggtactga cggaggccgg cgagctcctg cttctgcatg
     5221 tcgaagcgat ggccggggcg gctggagaaa tcgaaacagt gggagggccg gtcacctcag
     5281 ccttgaccgg aaccttgcga gtcagcgtag ctgaaggatt tgggagttgg ttcctcgccc
     5341 ggcacgtgcc tgagttcgta gctgccaatc caaatcttac gctcgacttg gtcgccaaca
     5401 gtggcttcct ctcggtgacg aagcgggaag ccgacatcgc ggtgatgctg tcgcgtccgc
     5461 ggactggtgc gctcatttcc ggaaagcttt ccgattacac tctgcaacta ttcgcaaccc
     5521 gggaatacct ggcgtgccgc ggcattcccc gcagctcgtc ggacctggcc gatggccacc
     5581 gtttcattgg ctacatcccg gacctgatct atgcacccga gctcaagtat ctcgacgagg
     5641 tgcagcctgg tctccgaccg gaaattcgct cctctagcat caatgcgcag tatcatctgg
     5701 tcgcatccgg cagcggcatc gccgtgcttc cttccttcat tggaaaggtc gacccgcagc
     5761 tccaggtcct gctcccggat ttccggatcg tgcgctcgtt ctggctggtc acacacaagg
     5821 atacgcacca gttggcgagg gtgcgggccg ggcgtgaatg gatcgtggac atggctcgcc
     5881 ggcaccggtc cgcgctcgtc gccgagtgag gtgatctgcc cccagtggga caggacccgg
     5941 tgctgattgg atcggggccg ggttgatcaa ggacaccggg a
//
LOCUS       DATNBV010000550         2306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_620367,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000550 DATNBV010000000
VERSION     DATNBV010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620367"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VN627_13045"
     CDS             complement(<1..1077)
                     /locus_tag="VN627_13045"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HXD04223.1"
                     /translation="MAEHRTESDSLGEIAVPASAYWGAQTQRSLENFPFAQAERMPLP
                     IVHALALIKQAAARVNRAPGPAPGLAPDLAHGLAPDLAQAIEQAAAEVAAGRHDDQFP
                     LAIWQTGSGTQTNMNVNEVIAGRANELLTGTRGGKAPVHPNDHVNRGQSSNDAFPTAL
                     HVAAAREVTQRLLPSLKRLHHALADKAAEWAAIVKIGRTHLQDATPLTLGQEFSGYAA
                     QLDRCRERLAHALHEELMPLAQGGTAVGTGLNAPEHFAADMAGQLATLTGLPFRPAPN
                     AFEALASHDPLVQLGGTLTTLAVALTKIANDIRLLGSGPRSGLGELDLPANEPGSSIM
                     PGKVNPTQAEMLTMVAAQVIGNGQA"
     gene            1160..1384
                     /locus_tag="VN627_13050"
     CDS             1160..1384
                     /locus_tag="VN627_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04224.1"
                     /translation="MQQQPIRPDPVSGPEPEDVPGGGGRAGGPPPDPPFRGRNAGVGT
                     GRPTEDAAVNNGRAGQKGHDDGTDGWNSNT"
     gene            1384..2142
                     /locus_tag="VN627_13055"
     CDS             1384..2142
                     /locus_tag="VN627_13055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXD04225.1"
                     /translation="MTPRWPEVLWVVRHGQSAGNVARDLATEQGLPRIALEHRDVDVP
                     LSDLGREQASALGQWFAELVERPEVMLASPYVRAVQTAELFRAAGGCHPAERICIDER
                     LREKEFGILDGLTAHGIREFQPEQAAFRRNLGKFYHRPPGGESWVDVIFRLRALMDTV
                     SLHYGGRRVMIVAHQVVVLCLRYVIENLSEAEVLAIDKAGDVANCAITEYRFDPAQGR
                     DGGLVLSRYNVTAPMEQGHTAVTAEPDRMVAARG"
BASE COUNT          317 a          684 c          957 g          348 t
ORIGIN      
        1 cgcctgccca ttgccgatga cttgcgccgc caccatggtc agcatctcgg cctgggtggg
       61 attgaccttc cccggcatga tcgaggagcc gggctcgttg gcgggcaggt cgagctcgcc
      121 gaggcccgag cgcgggccgg agccgagcag gcggatatcg ttggcgatct tggtgagggc
      181 gacggccagg gttgtcagcg tgccgccgag ctggaccagc ggatcgtggc tggcgagtgc
      241 ctcgaaggca ttgggcgcgg ggcggaaggg gagtccggtg agcgtggcga gctggccggc
      301 catgtcggcc gcgaagtgtt cgggggcatt gaggccggtg ccgacggccg tgccgccctg
      361 ggccagcggc atgagttcct cgtgtagggc atgggcgagg cgctcgcggc agcggtcgag
      421 ctgggcggca tagccggaga attcctggcc gagggtgagc ggggtggcgt cctgcaggtg
      481 ggtgcggccg atcttgacga tcgcggccca ctcagcggcc ttgtcggcca gggcgtggtg
      541 gaggcgcttc aggctcggga gcaggcgctg ggtcacctcg cgcgcggcgg cgacgtggag
      601 cgcggtgggg aaggcatcgt tggacgactg gccgcgattg acgtggtcgt tggggtggac
      661 cggggccttg ccgccgcgcg tgccggtgag cagctcgttg gcgcggccgg cgatcacctc
      721 gttgacgttc atgttggtct gggtgccgga gccggtctgc cagatagcga gggggaactg
      781 gtcgtcgtgc cggccggcgg cgacttcggc ggcggcttgt tcgatggcct gtgcaagatc
      841 cggggccagc ccatgggcga gatcgggggc gaggccgggg gcgggaccgg gggcgcggtt
      901 cacccgggcg gcggcctgct tgatcagggc gagggcgtgg acgatcggga gcggcatgcg
      961 ctcggcctgg gcgaagggga agttttcgag gctgcgctgg gtctgcgcgc cccaataggc
     1021 ggatgccggg acggcgattt cgcccaggct gtcgctctcg gtccggtgtt cggccatcgg
     1081 ggcaagctcc ttagaggcca aggatagcag agggaacagc gcgggggcga ggggcgttcc
     1141 ggcctcgagg agatttgcga tgcaacagca gccgatcagg cccgatccgg tgagtgggcc
     1201 ggagccggag gacgtccccg gcggtggcgg gcgtgcgggc gggccgccgc cggacccgcc
     1261 gtttcgcggg cgcaatgccg gggttggcac cgggcggccg accgaggatg ccgcggtaaa
     1321 taatgggcgt gccgggcaga agggccatga cgatggcacc gacggctgga attcgaacac
     1381 ctgatgaccc cgcggtggcc agaggtgctg tgggtcgtcc gccacgggca gagcgcgggc
     1441 aatgtcgcgc gcgatctcgc gaccgagcag ggattgccgc ggatcgcgct ggagcaccgg
     1501 gatgtggacg tgccgctgtc cgacctcggg cgcgagcagg cctcggcgct ggggcagtgg
     1561 ttcgcggaat tggtggagcg gcccgaggtc atgctcgcct cgccctatgt gcgggcggtg
     1621 cagacggccg agctgttccg ggcggctggc ggctgccatc cggcggaacg gatctgcatc
     1681 gacgagcggc tgcgcgagaa ggagttcggc atccttgacg gcttgacggc ccacggcatc
     1741 cgcgaattcc agcccgagca ggcggcgttc cggcgcaacc tcggcaagtt ctatcaccgg
     1801 ccgccgggcg gggagagctg ggtcgacgtg atcttccggc tgcgggcgct gatggatacg
     1861 gtttcgctgc actatggcgg gcggcgggtg atgatcgtgg cgcaccaggt ggtggtgctg
     1921 tgcctgcgct atgtgatcga gaacctctcg gaagccgagg tgctggcgat cgacaaggcg
     1981 ggggatgtcg ccaactgcgc gatcaccgaa taccgcttcg atcccgccca ggggcgggac
     2041 ggcgggctgg tgctgagccg ttacaacgtg accgcgccga tggagcaggg gcataccgcg
     2101 gtgaccgccg agcccgaccg gatggtggcc gcgcgtggct gacgggcccg atgtaccggg
     2161 cagtacgccg ctcgacagca cctggctgcg cgccaacccc ctgccgctgc ctgacggcga
     2221 gaccgacaag aacgggcgcg ggcgggtgct ggccatcggc ggatgccgga ccgtgccggg
     2281 cggcatccag ctgaccgccg aagcgg
//
LOCUS       DATNBV010000551         3099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3513486,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000551 DATNBV010000000
VERSION     DATNBV010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3513486"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VN627_13060"
     CDS             <1..198
                     /locus_tag="VN627_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04226.1"
                     /translation="PGLLKPVGLMAADFPAMKEKVFERYPMMRSTAFERRMLFERPGG
                     RAARPARVASAYERASIAPGS"
     gene            536..1027
                     /gene="pal"
                     /locus_tag="VN627_13065"
     CDS             536..1027
                     /gene="pal"
                     /locus_tag="VN627_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXD04227.1"
                     /translation="MKYQMRFLQGWKLAAVVAVALSMGACAKNNVGADGAMASAATPG
                     SQQDFVVNVGDRVFFESDQTELSPQAIATLEKQAQWLQTYNRYSFTIEGHADERGTRE
                     YNIALGARRAQSVRSYLASRGIDANRMRTISYGKERPVAVCNDISCWSQNRRAVTVLN
                     AGA"
     gene            complement(1149..2021)
                     /locus_tag="VN627_13070"
     CDS             complement(1149..2021)
                     /locus_tag="VN627_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXD04228.1"
                     /translation="MYVTVNGVRLFFDVEGAKLVPDGPVMREKPVLLMLHGGPGADHS
                     IYRPAYSALADVAQIVYLDHRGNGRSEDGPRESWNLAQWGDDVRAFCDALGIVDPIVL
                     GASFGGMVALAYATRHPAHPSKLVLISTEAAGGSYRERRVALFERFGGPEVGALARRR
                     FLQPEAPLDQEAREAWRRLAIPVYTRIPRDPDMARRAISRPEVALHWFAKPGGESETF
                     NMFPALDRIRCPTLVIGGEDDPMHPIESQADLAAALPPSLVQFERFADCRHGVVPDAP
                     ERAMALLREFILKR"
     gene            2203..>3099
                     /locus_tag="VN627_13075"
     CDS             2203..>3099
                     /locus_tag="VN627_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04229.1"
                     /translation="MSSRFLNAAGAAAFAAMLALSAQTASAQITFPWERSQQQQGYPQ
                     GQQGYPQGQGQSDDADMEMRIQRLESQLRQLTGQNEELQYRNRQLEDRLRQLGAAPPA
                     PGGQPAQPNVAAAPPPVQPGPPQGQQGYPQQGYPQQAPRQQGYPQAQGGYDQQPQIAA
                     PAPIVQDPAAPPAGGRRRGDAFDPSQNPNAPGAPRALGGGQMPMSAEAAAGGAPGGRG
                     PGEPLNLGGPRDPGGALPPPAAAAPPPRGPGPGANAALTTLPPSATSKDEFDLGIGYM
                     QRKDYALAEETMKNFAQKYPSDP"
BASE COUNT          559 a         1036 c         1001 g          503 t
ORIGIN      
        1 cccggcctgc tcaagccggt cgggctgatg gcggcggatt ttccggcgat gaaggaaaag
       61 gtcttcgagc gctatccgat gatgcgctcg accgcattcg agcgacggat gctgttcgag
      121 cgccccggcg gccgggccgc acggccggcc cgtgtcgcct ccgcctacga gcgcgcctcg
      181 atcgctccag gttcctgaac gggacgggcc cgcgggcaga atcaactgcg tgcggccgtg
      241 tcggcttccc accgccggca accaggcaag acgcgtcatt ggccatcggg gccggggcgc
      301 atttcgcccc aatgccccgg acaaagcccc aaatatggtg atttgggcct gctgcgcgct
      361 tgccttcacc atcgcgccac atttacaaac cattaactat cgcggtcgct tttcacggtt
      421 tgtcggcatt tgatcgggtc tggcaaggtt ccatcaaggt tgacggaacg ttcgcttaac
      481 catcgacctg tagaccggat aacagtcgaa aaacgtgagc gttggaggct ccggaatgaa
      541 atatcagatg cgtttcctcc agggatggaa gctggcggct gtggtcgcgg tggcgctgtc
      601 gatgggcgcc tgcgccaaga acaacgtcgg cgccgatggt gcgatggcga gcgcggcaac
      661 cccggggagc cagcaggatt tcgtggtcaa tgtcggtgac cgcgtgttct tcgagagcga
      721 ccagaccgag ctgagcccgc aggcgatcgc cacgctggag aagcaggcgc agtggctgca
      781 gacctacaac cgctattcgt tcacgatcga aggccatgcc gacgagcgcg gtacccgcga
      841 atacaacatc gcgctcggcg cacgccgtgc ccagtcggtg cgcagctatc tcgcctcgcg
      901 cgggatcgac gcgaaccgca tgcgcaccat ctcctacggc aaggagcgtc cggtggcggt
      961 ctgtaacgac atctcctgct ggtcgcagaa ccgccgcgcg gtcacggtgc tgaacgccgg
     1021 cgcctgacgc gttcggactt tcgcagagtt tcaaaaccgg cgtcttcaga cgccggttta
     1081 acgacatttt aagtattttg gccaagtgga tggcggcgtt cgttaagaat gcttgccaag
     1141 aagttaatct agcgcttcag gatgaattcc cggagcagtg ccatcgcccg ttcaggcgca
     1201 tcgggcacca cgccgtgcct gcaatccgca aaccgttcga actgcacgag gctcggcggt
     1261 agcgcggcgg cgagatcggc ctggctttcg atcgggtgca tcggatcgtc ttcaccgccg
     1321 atcaccaggg tcggacaccg gatccggtca agcgccggga acatgttgaa cgtctcgctc
     1381 tcgccgcctg gtttggcgaa ccagtgcagg gccacctccg gacggctgat cgcacggcgc
     1441 gccatatccg ggtcccgcgg aatgcgcgta tagaccggga tcgcgagccg cctccacgcc
     1501 tcacgcgcct cctggtctag aggggcctcg ggctgcaaga accggcggcg tgccagtgcg
     1561 ccgacttccg ggcctccgaa gcgctcgaac agcgccacac ggcgctcccg ataggaaccg
     1621 ccggcggctt cggtgctgat caatacaagc ttcgaggggt gggccggatg gcgggtggca
     1681 taggccagcg caaccatgcc gccgaacgag gcgccgagca cgatcggatc gacgatgccg
     1741 agcgcatcac agaacgcgcg cacgtcgtcg ccccactgcg ccagattcca gctctccctg
     1801 gggccatcct cgctgcgacc gttgccgcga tggtcgagat agacgatctg tgcgacgtcg
     1861 gccaaggccg agtaggcggg ccggtagatc gagtggtcgg cgccgggccc gccatgcagc
     1921 atcagcagca ccggcttttc ccgcatcacc ggcccgtccg gaaccagctt tgcgccctcg
     1981 acatcgaaga acagccgcac cccgttcact gtcacgtaca tgaacgagac tccctcgatt
     2041 tgtgaacggt ccgccgggcc ggacggcttg tcttgtagta gatactaaac cataccggag
     2101 cagcgcgagc cgagtcggaa atgccagtca caattctggc gtactggctc tctcaaggtg
     2161 ttctgctttc aatcgacacg tggttttcgt cgtcagggca aaatgtcatc cagattcctc
     2221 aatgctgctg gtgccgcggc gtttgccgcg atgctggcat tgtctgcgca gacggcgtcc
     2281 gcgcagatca cgttcccttg ggagcgctcg cagcaacagc aaggttaccc gcaggggcag
     2341 caaggctatc cgcagggcca ggggcagtcc gacgacgccg acatggagat gcggatccag
     2401 cggctggaaa gccagcttcg gcaactgacg ggtcagaacg aggaactgca gtaccgcaac
     2461 cgccagctcg aagatcggct gcgccagctc ggcgccgcac cgcccgcgcc gggtggccag
     2521 cccgcacagc ccaacgtggc cgctgccccg cctccagttc agccgggccc gccgcagggg
     2581 cagcagggct atccgcaaca aggttatccg cagcaggcgc ctcgccagca gggctatccg
     2641 caggcccaag ggggctacga ccagcagccg cagatcgccg ctccagcgcc gatcgtacag
     2701 gacccggcag ccccaccggc cggtggccgt cgccgtggcg atgccttcga cccgagccag
     2761 aatcccaacg ccccgggcgc gccgcgcgcg ctcggcggtg gtcagatgcc gatgtcggcc
     2821 gaagcggcgg ccggcggcgc tccgggcggg cgcgggccgg gcgagccgct caatcttggc
     2881 ggtccgcgcg atcccggtgg cgcgttgccg ccgcctgctg ctgccgcacc gccaccgcgc
     2941 ggccccgggc cgggcgccaa cgccgcgttg accacgctgc cgccctcggc cacgtcaaag
     3001 gacgagttcg atctcggcat cggctacatg cagcgcaagg actatgcgct ggccgaagag
     3061 accatgaaga atttcgcgca aaaatacccg agcgatccg
//
LOCUS       DATNBV010000552         2105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_947415,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000552 DATNBV010000000
VERSION     DATNBV010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947415"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VN627_13080"
     CDS             <1..556
                     /locus_tag="VN627_13080"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD04230.1"
                     /translation="GRPVAIHGGAGGWANPPSTGGGNANLYFEAHTLWPQAVMTHLIS
                     FISHGVFDKFPRLRLLLIEAGAAWLPSLLWRCDTDYKGLRREVPWLRRLPSEYVAEHV
                     WLTTQPLEISPRREQIVELLSWINGSERLVYSSDYPHWDADEIKHVSAQLPPSWHRKV
                     FFENAMELYGWAETALPSLQTVDA"
     gene            553..930
                     /locus_tag="VN627_13085"
     CDS             553..930
                     /locus_tag="VN627_13085"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HXD04231.1"
                     /translation="MSVDVGAVGDFPCERVRIVDIDRREIGIVHWQDRFYAVSNLCAH
                     QGGPLCRGVLSARLTAAEPGELTLDQSAPLLACPWHGWEFDLRTGRAILDPKVRVRTF
                     PARVAEGRVLVEMNDASPISNPS"
     gene            972..>2105
                     /locus_tag="VN627_13090"
     CDS             972..>2105
                     /locus_tag="VN627_13090"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXD04232.1"
                     /translation="MSTVRAAVMERPGRIAVQEFPMPEPQEGAVLMQVSYSGICGTDK
                     HTFRGESKQYAGTAHERDLTYPLICGHENVGRVAALGGTVRDSDGFPLKVGDRIVPGA
                     NVPCGECHFCVNDYPYYFCERMEDYGNSLHCGRAPHLFGGWAEHMYLLPGTPIFRVPD
                     DLPDSVAVLTEIMAVTHGVETALAVLGMHGGPRFGGTVAVLGVGPLGLCHLIKAKLLG
                     AATLIATDRFPSRLAVAQSFGAELVIPVDETEPEERIARVRERTGGLGADIVLSCSGV
                     PSTFVEALRMVRVGGVVVEAGTFVDMGPVAINPNADICTRNVTVIGVGGETATTYMPA
                     MRLMAANLKNLPLERFVSHRFKLDQAQHAVELAQTDAAMKVVMA"
BASE COUNT          369 a          640 c          700 g          396 t
ORIGIN      
        1 cggccgtccg gtggcgatcc acggcggagc cggtggttgg gccaacccgc cgagcaccgg
       61 cggcggcaat gccaacctct atttcgaggc gcacacgctt tggccgcagg cggtaatgac
      121 ccatctcatc tcgttcatca gtcacggggt attcgacaag tttccaaggt tacgcctgct
      181 gctgatcgag gcgggagcgg cttggttgcc cagtctgctc tggcgctgcg ataccgacta
      241 caagggcttg cgccgggagg ttccatggtt gcgtcggctg ccgagcgaat atgtcgccga
      301 acacgtctgg ctgacgaccc agccgctcga gatctcgccc aggcgcgagc agattgtgga
      361 gctgttgagc tggataaacg gttcggaacg cctcgtttat tcctcggact atcctcattg
      421 ggacgccgac gagatcaagc acgtttcagc ccagcttccg ccaagctggc accgcaaggt
      481 cttcttcgag aacgcgatgg agctttacgg ctgggccgag acggccctgc cctcgctgca
      541 aacggttgat gcatgagcgt ggatgtcgga gcagtcggcg atttcccctg cgagcgggta
      601 cgcatcgtcg atatcgaccg gcgagagatc ggaatcgtgc actggcagga ccggttttat
      661 gccgtgagca atctatgcgc tcaccagggc ggccccttgt gtcgcggcgt cttgagcgcg
      721 cgtctgacag ccgccgaacc gggggagctc acgctcgatc agtcggcgcc gttgcttgcc
      781 tgtccatggc atggctggga attcgacctt cgcaccggaa gagcgatcct cgatccgaaa
      841 gtccgggtgc ggacctttcc ggcgcgggtg gccgagggcc gcgtgcttgt cgagatgaat
      901 gatgcaagtc cgatatcaaa cccaagctga ggcatgactg tgagtctcca gctcgaggga
      961 ggagccgcca catgagcacc gttcgcgccg ccgtcatgga gagaccgggc cggatcgccg
     1021 ttcaggaatt cccgatgccg gaaccgcagg agggcgcggt gctgatgcag gtcagctatt
     1081 cggggatctg cggcaccgac aagcacacgt tccgcggcga gagcaagcaa tacgccggca
     1141 ccgcgcatga gcgcgacctt acctacccgc tgatctgcgg ccatgagaat gtcggccggg
     1201 tcgcggctct cggaggcacg gtgcgcgaca gcgacggctt ccctctcaag gtcggggacc
     1261 ggatcgtgcc gggcgccaac gtgccctgcg gagagtgcca tttctgcgta aacgactacc
     1321 cctattattt ctgcgagcga atggaagact acggcaacag cctgcattgc gggcgggcgc
     1381 cgcatttgtt cggaggctgg gccgaacaca tgtacctcct gcccggaacg ccgattttcc
     1441 gggtgcccga tgacctcccc gacagcgttg cggtgctcac cgagattatg gcggtgacgc
     1501 acggcgtcga aaccgcgctt gcggtcctcg gcatgcatgg aggcccgcga tttggaggaa
     1561 cggtggcggt gcttggggtg ggcccgctcg gcctgtgtca cctcatcaag gccaagttgc
     1621 tcggtgcggc aactctgatc gctaccgacc gcttcccttc ccgcctcgct gttgcgcaga
     1681 gtttcggcgc cgaactggtt atcccggtcg acgagaccga gcccgaggag cggattgcgc
     1741 gggtgcgcga gcgcaccggc ggactgggcg ccgatatcgt gctctcgtgc agcggcgttc
     1801 ccagcacttt cgtggaagca ttgcgcatgg tgagggttgg cggcgtcgtg gtggaggcgg
     1861 gcacgttcgt ggacatgggc ccagtcgcaa tcaatcccaa tgccgacatc tgcacgagga
     1921 acgtcaccgt gatcggcgtc ggcggcgaga cggcaacgac ttacatgccc gcgatgcggc
     1981 tgatggcggc aaatcttaag aacttgccgc tcgagcgctt cgtcagtcat cgcttcaagc
     2041 tcgatcaggc gcaacatgcg gtggagctcg cgcagaccga tgccgccatg aaagttgtga
     2101 tggct
//
LOCUS       DATNBV010000553         4414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3128422,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000553 DATNBV010000000
VERSION     DATNBV010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3128422"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..592)
                     /locus_tag="VN627_13095"
     CDS             complement(62..592)
                     /locus_tag="VN627_13095"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HXD04233.1"
                     /translation="MNDNLAKFLKAATENIQEHLKLYIETDGGDEAYRRDMSPQGGDP
                     KTKTLVLKTVGRKSGRDVLAPLIFNTWGDDFIVVASKGGSDQSPAWYLNLVANPEVVV
                     QVRDKRYRCTARVAEGDERAKLWRFMAGYYPPYLEYQARTSREIPVVVLTPGEEVAEK
                     FVWHDGEGVDAAMRGK"
     gene            complement(727..2115)
                     /locus_tag="VN627_13100"
     CDS             complement(727..2115)
                     /locus_tag="VN627_13100"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXD04234.1"
                     /translation="MLGQVKDRAGTPASGLRDEWRAGKGELPAARYCSKEFAALEAEQ
                     LWGKAWQMACRIDQLPEVGSYVVYDILDQSVVVVRVDEVTVKAFHNVCPHRATALAVD
                     TGRFQLKQIVCPFHGWKWNLSGENTFVADLQEFRGGCMTNADADLTEVHCEVWAGFVY
                     ISMAPDPVPFDEFVAPIRELVDGVLLGEMKFHYHYQARVNANWKVALEAFIEAYHVPQ
                     THPQLTPGSAADFTALYTYEPMANGHGLFHSSGANSVGRISREQLAKLDKGQQVEALL
                     RGLQTLHTGQDAQAHIEELEIVRTLRNRDIPEGMTVGAYFQTVIREHYAAQGRAIGTF
                     ENLSKVGDMHIFPHVVFLPTYGNAVMYRARPSPDNDPDWCIFDMYAIRTYPEGMAPPP
                     WQTIHAEGRLDQAKTWFLIPSQDFSSIVRQQRGMHSLAFRSAVLSARQESLILNLHHE
                     IDRYLDGEVPAA"
     gene            complement(2125..2472)
                     /locus_tag="VN627_13105"
     CDS             complement(2125..2472)
                     /locus_tag="VN627_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXD04235.1"
                     /translation="MLLTEHDGAGAGVAIVRVDPMGFDVAVPAGTSLMAAAAAAGVRW
                     PSLCGGDAQCGVCAVEVIESGAPLAEPSLAEGQMLARLATRPRQGGTMRLACQWRPSA
                     GARVAKPGVRAPK"
     gene            complement(2459..3868)
                     /locus_tag="VN627_13110"
     CDS             complement(2459..3868)
                     /locus_tag="VN627_13110"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXD04236.1"
                     /translation="MSDAAEVPAAELPHPALPPEQPAAPQVRPWDVLPKDVVPPPATL
                     IEDRSNWVPEVKPIDLTRYTSPEFHKLEVERMWSRTWQFAVWDGDIPNPGDIYVYRNV
                     GRSVLVVRQRDGSVKAFVNSCLHRGRELCSEKTHQSQLRCPYHAFTWGLEGDLKWIPS
                     QWDYPQIEKDKFSLPEVRVEQWNGFWFVNFDPAAPSLDEYLGDMVDHWKAWDWSDRYR
                     SVTVARKLNCNWKAALDAFIETLHVYASHPEAAYLTPDSATQYDTWPDQPHFSRFHSI
                     VGWASANLDPEPSQQEVLDSYTSVYLPEMFGTPEGDLQEGEGAREALRRIAVDVYRDR
                     MGVDVSQMPNSELLDGTEYMIFPNFIIWPSTANPLVYRFRPGNGPDECLWETSLYLQY
                     QGERPAPGPVYELEVGEPMRDIPELGYVGPILQQDCDNLEFMQDGMKASVTGEANVAR
                     YQESRLRHYHETLGKYVAD"
     gene            complement(4229..>4414)
                     /locus_tag="VN627_13115"
     CDS             complement(4229..>4414)
                     /locus_tag="VN627_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04237.1"
                     /translation="WPGGFGIAQGWTTMAGLTGPVCLGMRICCKVLLFRIRDMALTRQ
                     TPMKSSSYVGRNLTTNC"
BASE COUNT          757 a         1483 c         1399 g          775 t
ORIGIN      
        1 accggcaccg ccggcaacgc ctgaaggatg accccggccc gccggccggg cttcattgcg
       61 gctacttgcc gcgcatcgcc gcatccacgc cctcgccatc gtgccagaca aacttctccg
      121 caacttcctc gccgggggtc agcaccacga ccggaatttc gcgcgacgtg cgcgcctgat
      181 attcgaggta aggcggataa taaccggcca tgaaccgcca cagctttgcc cgctcatcgc
      241 cctcggccac ccgcgccgtg cagcgatagc gcttgtcgcg cacctggacc acgacctcgg
      301 gattggcgac gaggttgaga taccaggccg ggctttggtc agacccacct ttggaggcga
      361 cgacgatgaa gtcgtcgccc caggtgttga aaatcagcgg ggcaaggacg tccctgccgc
      421 tcttgcggcc gacggtcttc agcaccagcg tcttggtctt gggatcaccg ccctgggggc
      481 tcatgtcgcg acgataggct tcgtcgccgc catcggtctc gatgtagagc ttgaggtgct
      541 cctggatgtt ttccgtcgcg gctttcagga acttggccaa gttgtcgttc accatgcgtc
      601 tccccgttgg aatggcggcc acttgcggac taatacggca gcgtgcggaa ccgccaatcg
      661 agctggaaag caacctccct gaccagccga ccatccgcag gtcatccacc cggcgcccca
      721 taaggctcag gcggcgggaa cctccccgtc cagatagcgg tcaatttcgt ggtgaaggtt
      781 gaggatcagg ctttcctgcc gggccgacag cacggccgag cggaaggcaa gcgaatgcat
      841 gccgcgctgc tggcgcacga tgctggagaa gtcctggctg gggatcagga accaggtctt
      901 cgcctggtcc agccgcccct cggcatggat ggtctgccac ggcggcggcg ccatcccctc
      961 aggataggtg cggatcgcgt acatgtcgaa gatgcaccag tcgggatcgt tgtcggggct
     1021 gggccgcgcg cgatacatca ccgcattgcc ataggtcggc aggaacacga catgcgggaa
     1081 gatgtgcatg tcgccaactt tcgagaggtt ctcgaacgtg ccgatcgccc gcccctgcgc
     1141 cgcatagtgc tcgcggatca ccgtctggaa ataggcgccc acggtcattc cctcgggaat
     1201 gtcgcggttg cgcagggtgc ggacgatctc gagctcctca atgtgagcct gcgcgtcctg
     1261 cccggtgtgc agcgtctgca gcccccgcag cagcgcctcg acctgctgcc ccttatcgag
     1321 cttcgccagc tgctcgcgcg agatgcgccc cacgctgttc gcgcccgagg aatggaacag
     1381 gccgtgacca ttggccatcg gctcgtaggt gtatagcgcg gtgaagtcgg ccgcgctccc
     1441 gggcgtcagc tgggggtggg tttgcgggac gtggtaggcc tcgatgaagg cctccagggc
     1501 caccttccag ttggcgttga cccgggcctg gtagtggtaa tgaaacttca tctcgcccag
     1561 cagcacgccg tcgacgagct cgcggatcgg cgccacgaac tcatcaaacg ggaccggatc
     1621 gggcgccatg ctgatgtaga cgaacccggc ccagacctcg cagtggacct cggtcagatc
     1681 cgcgtccgca ttcgtcatgc agccgcccct gaactcctgc aggtcggcca cgaaggtatt
     1741 ctcgccggac aggttccact tccagccatg gaatgggcag acgatctgct tgagctggaa
     1801 ccgccccgtg tccaccgcca gcgccgtcgc gcgatggggg cagacattgt ggaatgcctt
     1861 gaccgtcacc tcgtcgacgc ggacgaccac gacggactgg tcgaggatat cgtagaccac
     1921 gtagctgccg acctcgggca actggtcgat ccggcaggcc atctgccacg ccttgcccca
     1981 cagctgctcg gcctcaagcg cggcgaattc cttcgagcag tagcgggcgg ccggcagttc
     2041 gcccttgccg gcccgccact cgtcgcgcaa gccacttgcc ggtgttccgg cgcgatcctt
     2101 aacctgacca agcatttcgc acctctactt cggggccctt acgcccggct tcgccacgcg
     2161 ggccccggcg gaaggtcgcc actggcaggc cagccgcatc gtgccgccct gtcggggccg
     2221 cgttgccagc cttgcgagca tctgcccttc ggcaagcgac ggctcggcca gcggcgcacc
     2281 gctctcgatc acttccaccg cgcagacgcc gcactgcgcg tcgcccccgc acaggctcgg
     2341 ccagcggact cccgccgcgg ctgccgcggc catcaggctg gtcccggccg gaacggccac
     2401 gtcgaacccc atcgggtcga cccggacgat cgcaacaccg gcaccagctc cgtcgtgctc
     2461 agtcagcaac atacttcccg agcgtctcgt gatagtggcg cagccggctt tcctggtacc
     2521 gcgccacgtt ggcctcgccg gtcacactgg ccttcatccc gtcttgcatg aactcgagat
     2581 tgtcgcagtc ctgctgcagg atcggaccga catagccgag ctcgggaatg tcgcgcatcg
     2641 gttcccccac ttccagctcg tagaccgggc cgggtgccgg gcgctcgccc tggtactgca
     2701 ggtaaagcga ggtctcccac aggcattcgt ccggcccgtt gccgggacgg aagcggtaga
     2761 ccagcggatt ggcggtcgac ggccagatga tgaaattcgg gaatatcatg tactcggtgc
     2821 cgtcgagcag ctcggaattg ggcatctggc tgacgtcgac gcccattcgg tcgcggtata
     2881 cgtccaccgc gatccgccgc agcgcctcgc gcgcgccctc gccttcctgg agatcgccct
     2941 cgggcgtgcc aaacatctcg ggcaggtaga ccgaggtgta gctgtcgagc acttcctgct
     3001 gggagggttc gggatccaga ttggccgacg cccagccgac gatcgagtgg aatcggctga
     3061 agtgcggctg gtcgggccag gtatcgtact gcgtggcgct gtccggcgtc agataggcag
     3121 cctcggggtg cgaggcatag acgtgaagcg tctcgatgaa ggcgtcgagc gcggccttcc
     3181 agttgcagtt gagcttgcgc gcgaccgtga ccgagcggta gcggtcggac cagtcccagg
     3241 ctttccagtg atcgaccatg tcgcccaggt attcatccag gctcggcgcg gccggatcga
     3301 agttgacgaa ccagaacccg ttccactgtt ccacccgcac ctccggcagg ctgaacttgt
     3361 ccttctcgat ctgcgggtag tcccactgcg aggggatcca cttgagatcg ccttccagcc
     3421 cccaggtgaa ggcatggtaa gggcagcgca gctggctctg gtgagtcttt tcgctgcaca
     3481 gctcgcggcc gcgatgcagg cacgaattga cgaaggcttt caccgagccg tcgcgctggc
     3541 gcaccaccag cacggaccgg ccaacgttgc gatagacata aatgtcgccc ggatttggga
     3601 tatcgccgtc ccacaccgcg aactgccagg tgcggctcca catccgctcg acctcgagct
     3661 tgtggaactc ggggctggta tagcgcgtca ggtcgatcgg cttgacctcc ggcacccaat
     3721 tcgaccggtc ttcgatcagc gtggcgggag gcggcaccac gtccttgggc agcacgtccc
     3781 aggggcgcac ctgcggcgcc gccggctgct cgggcggaag cgccggatgg ggaagttcgg
     3841 cggcagggac ttctgcggca tcggacacgg gcttactcct ggtttggcag agggcgcgga
     3901 agggctgacc gcgaatgctg cagcatcggc ttgtcaagcg cgcgcgccat cctgtcatcc
     3961 ttccatcggg cgcgcagcgg caagatcctg ccgctggtca accgtcctgc aatagtagtg
     4021 gcaagcaagc caacccacca gccctgctca gaagcaccac acagatgatg gagtgcgtat
     4081 cgcgggatcc agtgccgccg gaacgacgcg atgacagggc aatgcgagcc cgcgcggaga
     4141 cggttggacg accggtcgcc tccgcccggg cttggaccgc gcggcgtggc tgtcaggccg
     4201 tgcagcccga caaagttccc ttgctcacct aacagttggt agttagatta cggccgacat
     4261 atgatgaact cttcatggga gtctgcctcg tcagcgccat gtcccgaatt cggaaaagca
     4321 ggaccttgca gcagatgcgc atccccaggc acaccggacc agtcagcccc gccatggtgg
     4381 tccagccttg agcaattccg aagccgcccg gcca
//
LOCUS       DATNBV010000554         4372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3129443,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000554 DATNBV010000000
VERSION     DATNBV010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3129443"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VN627_13120"
     CDS             <1..492
                     /locus_tag="VN627_13120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXD04238.1"
                     /translation="EDGTRTGVAGAGSIMDFGRSGTPDAILAPGSEPAPILLVEDEIL
                     IRLANADWLRDAGYAVIEAANAAEALDVIATGQVLALLATDITMPGALDGLALAAATR
                     MRRPGLPVLLLSAHVPDDYSAIAEAALSKPFGPSQLVAKVAALIGAPWQTTNPVTGAH
                     KAC"
     gene            447..854
                     /locus_tag="VN627_13125"
     CDS             447..854
                     /locus_tag="VN627_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXD04239.1"
                     /translation="MADDEPRDRSPQSLLIAESDVLVRHAIADYLRRCGYGVIEAASF
                     EEARTVLEGLPEKPQLVLSDVELGGEGNGFDLRRRAAREWPEIEFVLAGNVAAAAQAA
                     GDLCERGPHLSRPYDPQLVLDRIRRALATRTAR"
     gene            999..2126
                     /locus_tag="VN627_13130"
     CDS             999..2126
                     /locus_tag="VN627_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04240.1"
                     /translation="MNAPIRSAEFIGLIGVVANLALYNTPIKGAQALDALHYLGLSSI
                     RTTLSSGLLQAGSVADRMALAGVRLDVLMGGARPLAESLGYATTFARAHPNSLVALEG
                     PNEINNWPIAYNGLTGVKAAIAFTNALAAGAAGDPALAGAAIYDFTGSWRTAETSADA
                     AGFTNIHPYPQRGAQPFDWLKQAVQGAAVLGKGLVITEAGYTTATGQSAFEGVDPQTQ
                     AKLTLNLLADARLLGASRTFLYQLGDTGAAGSADSGFGLFDAQLQAKPVATAIHNLTA
                     ILADPGGQAGSFATHGLDYTLSGLPAGAHSLLAEKSNGTYELLVWAEPDIWDEAADRP
                     IAAARAEVTVRLGGSARMAIYDPLVSPTPWPARRAAASPFR"
     gene            2123..2674
                     /locus_tag="VN627_13135"
     CDS             2123..2674
                     /locus_tag="VN627_13135"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HXD04241.1"
                     /translation="MSDHVMVVEISGLAPGPVVRPATFDPAILLNGTTGADRLVGGNN
                     DDVLAGMPGNDTLLGGAGDDRLIGGLGADELWGGAGADRFVLQQSGQSSLKAMDTIRD
                     FSPGEGDRIDLSAVDAEISVTGNQAFRLGGSSFTKVAGELIQFDTATGLSVQGDVDGD
                     GRADFALLLAGSHAPLGADAFVL"
     gene            complement(2682..3395)
                     /gene="radC"
                     /locus_tag="VN627_13140"
     CDS             complement(2682..3395)
                     /gene="radC"
                     /locus_tag="VN627_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HXD04242.1"
                     /translation="MFADGNGKAPPVDAAHDPSGHRARLRKRLVDGGIDALGDHEVIE
                     LLLMGAVPRRDMKPLARALIQRFGSLAGVLNADPQTLARHPGMGEATAAALRIVAVAA
                     RRMARQAVHEQPVLGSWQALIDYLTIDMAHLTRERVRVLYLNTRNMLIHDDHLGDGSI
                     DEATIHPREVIHKAMDLGASAVILVHNHPSGSPEPSRADIQITQRIAEAGRLLNITVH
                     DHVIIGRQGHVSLRAQGLI"
     gene            complement(3470..>4372)
                     /locus_tag="VN627_13145"
     CDS             complement(3470..>4372)
                     /locus_tag="VN627_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD04243.1"
                     /translation="ASLMRLPLRGAARKAILPGIRYETAGRMERAMDLGIKGKSALVL
                     GGSRGIGRATARELAQAGANVAIVARNQAKIEEAVAELGALGVQACGIAADLFDIRSY
                     DRVVAECTASLGAPDIAIYNLDPPPPGGFYDIDEDQLAQAYQIVVLNYSRMLRLVLPH
                     MRARKWGRVVTIGSGTAKELVRGGFGWPFHYILANSSRVAATAFAKTVAAEVAADGVT
                     INTIATGYIRSEQQEAWTANRASDQDQAAADFEAAMLANIPMGRTGTTQEMASLAAFL
                     CSDLASYTTGEIISCSGGSANSIL"
BASE COUNT          706 a         1407 c         1512 g          747 t
ORIGIN      
        1 gaagacggca ccaggacggg ggtcgccgga gcagggtcga taatggactt cggcaggtcg
       61 ggcacgcctg atgcgattct tgcgccgggc agcgaaccag cgccgattct gctggtcgag
      121 gatgaaattc tcatccgcct cgccaatgcc gattggctgc gcgacgccgg ctatgctgtg
      181 atcgaggcag ccaatgctgc cgaggcgctt gacgtgatcg ccaccggaca ggtcctggcg
      241 ctgctggcga ccgacattac catgccaggt gcgcttgatg gcctggcgct ggctgcggca
      301 acgcggatgc ggagaccagg cctgccggta ctgctgctct cggcccatgt gccggacgac
      361 tattcggcca ttgccgaagc tgcgctctcg aaacctttcg gaccttcgca gcttgttgcc
      421 aaagtagcag ccctgatcgg agcgccatgg cagacgacga accccgtgac cggagcccac
      481 aaagcctgct gatcgccgaa agcgatgtgc tggtgcgcca cgccatcgcc gattacctgc
      541 gccgatgcgg ctacggggtg atcgaggcgg cctcgttcga ggaggcccgc acggtgcttg
      601 aaggtctgcc cgagaagccg cagctggtgc tcagcgatgt ggaactcggc ggcgagggca
      661 acggcttcga cctgcgccgg cgggcggcga gggaatggcc cgagatcgaa ttcgtgctgg
      721 ccggcaatgt tgccgcggcg gcgcaggcgg cgggcgacct gtgcgagcgc ggcccgcatc
      781 tttcccgccc ctatgatcct cagctggtgc tggacaggat ccgtcgggca ctggcgacga
      841 gaactgcgcg ctgaaacacc gttatcgcct tattcgcaag ttaatattca aatatgtatt
      901 tggcgaaggt gttaaacggt ggttttcctt gcctatgaac tcacgtgaaa tatggagggc
      961 atacctgtaa cggcagaagc catgcaggat ttaagcccat gaatgctccg atccgttctg
     1021 ccgaattcat tggattgatt ggcgtggttg ccaatctcgc gctctataac accccgatca
     1081 aaggcgcgca ggcgcttgac gcgctgcatt acctgggtct ctccagcatt cgcaccaccc
     1141 tttcgtcggg gctgttgcag gccggcagcg tggccgaccg gatggcgctg gcgggcgtca
     1201 ggctcgatgt cctgatgggc ggcgcccgtc cgctagccga gagcctgggc tatgcaacca
     1261 cctttgcccg cgcccacccc aattcgctcg tcgcactcga aggccccaac gagatcaaca
     1321 actggccgat cgcctacaat gggctgaccg gagtgaaggc ggcgattgcc ttcaccaatg
     1381 cgcttgccgc cggggccgcc ggcgatccgg cgctcgccgg cgccgcgatc tacgatttca
     1441 ccggctcatg gcgaacggcg gagaccagcg ccgatgccgc gggtttcacc aatatccatc
     1501 cctatcccca gcgcggggcc cagccgttcg actggctcaa gcaggcggtg cagggcgcgg
     1561 cggtgctcgg caaggggctg gtgatcaccg aggccggcta taccacggcg accggccaga
     1621 gcgccttcga gggcgtcgat ccgcagaccc aggccaagct cacccttaac ctgctggccg
     1681 acgcgcggct actcggcgcc agccgcactt tcctgtacca gcttggcgac acgggcgcgg
     1741 ccggttcggc ggattcgggc ttcgggctgt tcgatgcgca gctgcaggcg aagcccgtgg
     1801 cgaccgcgat ccataatctg accgcgatcc tcgcggatcc aggcgggcag gccggcagct
     1861 ttgccacgca tggcctggac tacacgctct cgggcctgcc ggcgggcgct cattcgctgc
     1921 tcgcggaaaa gagcaacggg acctacgaac tgctggtctg ggccgagccc gacatctggg
     1981 acgaggccgc ggaccggccg attgccgctg cccgcgccga ggtgaccgtg cggcttggcg
     2041 gctccgcccg gatggcgatt tacgatccgc tggtctcgcc aaccccctgg ccagcgcgac
     2101 gggcagcagc atcgccgttt cggtgagcga ccacgtcatg gtcgtcgaga tttcaggcct
     2161 tgcgccgggg ccggtcgttc gcccggctac gttcgatccg gcgatcctgc tcaacggcac
     2221 caccggggcc gaccggctgg tgggcggcaa caacgacgat gtcctcgccg ggatgcccgg
     2281 caacgatacc ctgctgggcg gcgcgggcga cgaccggctg atcggcggac tgggcgccga
     2341 cgagttgtgg ggcggggccg gtgccgaccg gttcgtgctg cagcagtcag gccaatcgtc
     2401 cctgaaggcg atggacacga tccgggattt cagcccgggc gagggcgacc ggatcgacct
     2461 gtcggcagtc gatgccgaga taagcgtcac gggcaatcag gctttccggc ttggcggcag
     2521 ctccttcacc aaggtggcgg gcgagcttat ccaattcgat actgcgacgg ggctatcggt
     2581 ccagggtgac gtggatggag acggcagggc cgatttcgca ctgctgctgg ccggcagtca
     2641 cgccccgctc ggcgcggatg ccttcgtgct ttagttaatg cctatatcag cccttgcgcg
     2701 cgcagggaga catggccctg ccggccgatg atcacgtgat cgtgcaccgt gatgttgagc
     2761 aggcgcccgg cctcggcgat gcgctgggtg atctggatat cggcgcggct cggctcgggc
     2821 gagcccgagg gatggttgtg gaccaggatc acggccgagg cgcccagatc cattgccttg
     2881 tggatcacct cgcgcggatg gatcgtcgcc tcgtcgatcg aaccgtcgcc aaggtggtcg
     2941 tcgtggatca gcatgttgcg ggtgttgaga tagagcaccc gcacgcgttc cctggtgagg
     3001 tgcgccatgt cgatcgtcag gtagtcgatc agggcctgcc acgagccgag cacgggttgt
     3061 tcgtgcaccg cctgccgggc catgcgccgc gccgcgacgg cgacgatccg cagcgccgcc
     3121 gcggtggctt cacccatgcc gggatggcgg gcgagggtct gcggatcggc gttgagcacg
     3181 ccggcgagcg agccgaaccg ctggatgagc gcgcgggcca gcggcttcat gtcgcggcgg
     3241 gggacggctc ccatcagcag cagctcgatg acctcgtgat cgcccagcgc atcgatcccg
     3301 ccgtcgacca gccgcttgcg caggcgcgca cggtggccgc tgggatcatg ggcggcgtca
     3361 accggcggtg ccttgccatt gccgtccgca aacagttcct gatcggccac gcccaccccc
     3421 tcgagcaagg gcgcagcttg ccgccatgcc gggccccgcg caagcgccgt cagaggatcg
     3481 agtttgccga accgccactg caggagatga tttcgccagt ggtataggag gcgaggtcgg
     3541 agcagaggaa ggcggcgagg gaggccatct cttgggtggt cccggtccgg cccatgggaa
     3601 tgttggcgag catggcggcc tcgaaatcgg cggcggcctg gtcctgatcg gaagcgcggt
     3661 tggcagtcca ggcctcctgc tgttcgctgc ggatataccc agtggcgatc gtgttgatcg
     3721 tcaccccgtc tgcggccact tcggcggcaa ccgtcttggc gaaggccgtg gcggcgacgc
     3781 gggaggaatt ggcgaggata tagtggaacg gccagccgaa cccgccgcgc accagttcct
     3841 tggccgtgcc ggagccgatg gtgacgacgc ggccccattt gcgcgcgcgc atgtgcggca
     3901 agaccaggcg cagcatgcgc gagtagttga ggacgacgat ctggtaagcc tgggcgagct
     3961 ggtcctcgtc gatgtcgtag aagccgcccg gcgggggagg gtcgaggttg tagatcgcga
     4021 tgtccggggc gccgaggctg gcggtgcact cggcaacgac gcggtcatag gacctgatgt
     4081 cgaacaagtc ggcggcaatg ccgcaggcct gtacgccgag ggcccccagc tcggccacgg
     4141 cctcttcgat cttggcctgg ttgcgcgcaa cgatggcgac attagcgccg gcctgggcca
     4201 gttcgcgcgc cgtcgcgcgg ccgatacccc gactgccccc cagcaccagg gctgacttgc
     4261 ccttgattcc aagatccatc gctctctcca ttcgcccggc cgtctcataa cggatgccgg
     4321 gcaggattgc ctttcgcgcc gcgccgcgca agggtagccg catgagcgac gc
//
LOCUS       DATNBV010000555         2736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3129791,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000555 DATNBV010000000
VERSION     DATNBV010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3129791"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VN627_13150"
     CDS             <1..729
                     /locus_tag="VN627_13150"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXD04244.1"
                     /translation="IIDAAEVFQEFGIPITIFVCAGWAASASKGDGSDLLARTCSMIE
                     WYSGEDFEISLQNRSYLFAEKTKAQNIENIARTGSLSVSEMIDICEQIDRRNTGQSSE
                     CCTWSELSQLASAKVAMGAHSVTHINLAAASPWRRKFEIAESRRVLASFFGDCSAFAY
                     SFGTDDAHNPETKRYLSECGYSSAFLTHSGFVTPLSDSFLYPRIALPDEPMSDREFRA
                     RVLGAGVFFRSMGELLCKFTPGRR"
     gene            complement(846..2105)
                     /gene="lysA"
                     /locus_tag="VN627_13155"
     CDS             complement(846..2105)
                     /gene="lysA"
                     /locus_tag="VN627_13155"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HXD04245.1"
                     /translation="MDHFQYVDGVLCAEDVPLPAISAAVGTPVYVYSRATLTRHARVF
                     LDGLGALPSKHVAFAVKSNPNVAVLRVLAREGLGADVVSAGEMDRALAAGMAPGSIVF
                     SGVGKTRAEMARALEAGIGQFNLESEEEGVELAQLAAAKGKIASAALRVNPDVDAGTH
                     GKISTGKAENKFGVPYDRAGGIYARLAALPGLSMRGLAVHIGSQLASLAPLEAAFTKM
                     GELMREIRSGGDAITHMDLGGGVGVPYKAGDVFPSPAEYGAMVALVTADWGVTLMFEP
                     GRVISGNSGVLVTEVVRVKEGVSAPFVVVDAAMNDLARPALYDAWHDFWAVEPSGAKM
                     VANIVGPICETSDTFAMGREIDAVGPGDLAVFRTAGAYGATMANTYNSRSLVPEVMVD
                     GDKWAVVAERIEPATILAAEHVPDWLD"
     gene            complement(2127..2378)
                     /locus_tag="VN627_13160"
     CDS             complement(2127..2378)
                     /locus_tag="VN627_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04246.1"
                     /translation="MLRRSLAALGLGLALPALTSCGQTAELKPRAGHELPVAPLGRGD
                     RPKPSELLVPSPQASPERSVELRSRSEPRQDDPFDLPPQ"
     gene            complement(2387..>2736)
                     /locus_tag="VN627_13165"
     CDS             complement(2387..>2736)
                     /locus_tag="VN627_13165"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685952.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXD04247.1"
                     /translation="VAATEFRTDRMRAAAELGYATATDLADWLVRQANIPFREAHHIT
                     GAAVKLAESRGVALDQLPLADLRAIDARIDERVYAALSVEASVAARCSHGGTAPAEVE
                     KRVAEACAALGLS"
BASE COUNT          475 a          943 c          835 g          483 t
ORIGIN      
        1 atcatcgatg ctgcagaagt gtttcaggaa tttggcatac ccatcacaat ttttgtttgc
       61 gcaggttggg cggcatctgc aagcaaaggc gacggatcgg atctactcgc gcgtacctgc
      121 tcgatgattg aatggtattc gggagaagac ttcgagatat ctttgcaaaa tcggtcttat
      181 cttttcgcag aaaaaaccaa ggcgcagaac attgagaaca tagcgcggac cggctcgtta
      241 tctgtctctg aaatgatcga catatgtgag cagatagatc gccggaatac cggccagagc
      301 agcgaatgct gcacctggtc ggaattgtcc caactggcgt ctgccaaggt cgccatgggt
      361 gcacattcgg taacgcacat caatctcgcc gctgcctcgc catggcgacg caagttcgag
      421 atcgcggaaa gccggagagt tttggccagt tttttcggag attgcagcgc gttcgcctat
      481 tcatttggta ctgatgacgc gcataatcct gagactaaac gctatttgtc ggaatgtgga
      541 tactcgtcag cttttttgac ccattccgga ttcgtaacgc cgttgagcga cagttttcta
      601 tatcctcgca tagcgctgcc agatgagccc atgagcgacc gtgaattccg cgcgcgagtg
      661 ctcggagcag gggtattctt tcggtcaatg ggcgagctgt tgtgcaagtt cacacctggg
      721 cgccgctgat caagagttgc tccacggcgt gcaagcatgt ccgcaatggc ggccttagcg
      781 gaaccgcagc tctcggcaaa gaccacacag aaaccagacg ccgccctcca ggccgctccc
      841 cccgctcagt ccagccagtc cggcacgtgc tccgccgcca ggattgtcgc cggctcgatc
      901 cgctcggcca ccacggccca cttgtcgccg tcgaccatga cctcgggcac cagcgagcgc
      961 gagttgtagg tgttggccat ggttgcgcca taggccccgg cggtgcggaa cacggcgagg
     1021 tcgcccggcc ccacggcatc gatctcgcgg cccatggcga atgtgtcgct ggtctcgcag
     1081 atcggtccga cgatattggc gaccatcttg gcgccgcttg gctccactgc ccagaagtcg
     1141 tgccaggcgt catacagggc cggccgggca aggtcgttca tcgccgcgtc gaccaccacg
     1201 aacggcgcgc tcacgccttc cttgacccgg acgacctcgg tcaccagcac gccggaattg
     1261 ccggagatca cccggcccgg ctcgaacatc agggtcaccc cccagtccgc cgtgaccagc
     1321 gcgaccatgg cgccgtattc ggccgggctc ggaaacacgt cgccggcctt gtacggcacg
     1381 ccgaccccgc cgccgaggtc catgtgggtg atcgcatcgc cgccgctgcg gatttcgcgc
     1441 atcagctcgc ccatcttggt gaaggctgct tccagcgggg cgaggctggc gagctggctg
     1501 ccgatgtgga ccgcaagccc gcgcatcgaa aggcccggca gggccgccag gcgcgcatag
     1561 atcccgccgg cgcggtcata gggcacgccg aacttgttct cggccttgcc ggtggaaatc
     1621 ttcccgtgcg ttcccgcgtc gacatcgggg ttcacccgca gcgccgcgct ggcgatcttg
     1681 cccttggcgg cggcaagctg cgccagttcg acgccttctt cctcgctctc gagattgaac
     1741 tggccaatcc cggcctccag cgcgcgggcc atttcggccc tggtcttgcc gacgccggag
     1801 aacacgatgc tgccgggcgc catgccggcg gccagcgccc ggtccatctc gcccgccgaa
     1861 accacgtcag cccccagccc ctcgcgcgcc agcacgcgca ggacggcgac attggggttg
     1921 gacttgaccg cgaaggccac atgcttgctc ggcagggcgc caaggccgtc caggaacacc
     1981 cgcgcgtgcc gcgtcagggt cgcgcgtgaa tagacataaa ccggcgtacc gacagccgcg
     2041 gagattgccg gaaggggcac gtcctccgcg caaaggacgc catcaacgta ttgaaaatga
     2101 tccatttttt atcctcgggc cggccgttat tgcggcggca ggtcgaaggg gtcgtcctgc
     2161 cgcggctccg accggctccg cagctccacg ctccgctcgg gcgaggcctg ggggctgggt
     2221 accagcagct cgctcggctt ggggcggtcc ccccgcccca gcggcgcgac cggcagctca
     2281 tgccccgccc gcggcttcag ctccgccgtc tgcccgcagg acgtcagcgc cggcaaggcc
     2341 agtccgagcc ccagggccgc aagagaacgc ctcagcatgt gtttcctcaa ctaagcccca
     2401 acgccgcgca cgcctcggcc acccgcttct ctacctcggc cggcgccgtt ccgccatggc
     2461 tgcaacgcgc cgccaccgac gcctcgaccg acagcgcggc atagacccgt tcgtcgatcc
     2521 gcgcatcgat cgcccgtagg tccgccagcg gcagctggtc cagcgcgacg ccccggctct
     2581 ccgccagctt caccgcagcg ccggtaatat ggtgcgcctc gcggaacggg atattggcct
     2641 gccgcaccag ccagtcggcg aggtccgtcg ccgtggcata gcccagctca gccgccgcgc
     2701 gcatccggtc ggtccggaac tcggtggccg cgacca
//
LOCUS       DATNBV010000556         3040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4425065,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000556 DATNBV010000000
VERSION     DATNBV010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4425065"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VN627_13170"
     CDS             complement(<1..294)
                     /locus_tag="VN627_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD04248.1"
                     /translation="MTRVAIVTGGSRGIGRAVCLALRDQGVSVVANYAGNDAAAQQFT
                     ADTGIPAYRWDVGDHEACLAGCAEVAAKHGPIDIVVNNAGITRDGVLHRMTFDD"
     gene            complement(436..1185)
                     /locus_tag="VN627_13175"
     CDS             complement(436..1185)
                     /locus_tag="VN627_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04249.1"
                     /translation="MVAITRGQMGAGSPALLLAGAVCLLALPSAVLAFSSDFGSAEDP
                     GLDAHSSMANFMPAQVDPRLARAITVPGLVGGQTFHFTPAGSATRPDRSVTVAVRVDA
                     VGGRAILVQAPSRDNQAALSALRIAPTAYSLGVSRGFQSFTMAGDKPRVDGPDLSSFN
                     LRSGVGGADEPSRLNARIAFDEREKTGRAPRTLEGNGEQTVDVGGSYRLTGNLNVTAG
                     VRYSQERDRVLQAFDGQKDGQAVYVGTQFRF"
     gene            1473..1961
                     /locus_tag="VN627_13180"
     CDS             1473..1961
                     /locus_tag="VN627_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HXD04250.1"
                     /translation="MTASRISGTSLSGRTVARCVRSLGTAGLAFVLAGGLAGCAHKER
                     PKADLAASRVTTIGVNSYLWRASLDALSFMPLVQTDSSGGVIVTDWYANPRNPGERVK
                     VTVTILDQDLRADALRVAASRQVAQNGTWVDAPVQAATVQRLEDVILTKARDLRRQAI
                     SG"
     gene            2000..>3040
                     /locus_tag="VN627_13185"
     CDS             2000..>3040
                     /locus_tag="VN627_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXD04251.1"
                     /translation="MTGTPSDSRPSDTRFDSASADLRWQRIWDEAQTFRADDKSAKPR
                     SYVLEMFPYPSGRIHIGHVRNYTMGDVLARAKRMQGFEVLHPMGWDAFGMPAENAAME
                     KGVHPGGWTRDNIANMKAQLKRLGFALDWSRELATCEPDYYGHEQALFLDLYAAGLVY
                     RKESEVNWDPVDHTVLANEQVIDGRGWRSGALVERRKLNQWFLKITDFAEELLEGLAT
                     LDKWPEKVRLMQENWIGKSQGLQFAFTLSNGESLEVYTTRPDTIFGASFVAVAADHPV
                     ARGVAGANPAAQEFIALCKQGGTTAAELETAEKLGFDTGLTAKHPFTGEALPVYIANF
                     VLMDYGTGAIMAV"
BASE COUNT          516 a         1067 c          968 g          489 t
ORIGIN      
        1 gtcgtcgaag gtcatccggt ggagcacgcc gtcgcgggtg atgccggcat tgttgacgac
       61 aatgtcgatc ggcccgtgct tcgccgcaac ctcggcgcag ccggcgaggc aggcctcgtg
      121 atcgcccacg tcccagcggt aggcgggaat gccggtatcg gccgtgaact gctgcgccgc
      181 cgcatcgttg cccgcgtagt tcgcgacaac ggaaacgccc tggtcccgaa gtgcgaggca
      241 gacagctctg ccgatgccgc gggaaccgcc ggtcacgata gctacgcgtg tcactttcgc
      301 ccctcctatt gtcgcattaa cgcaattgaa tagaggtgga ggctaggaaa cgccgccggt
      361 accggcaagc acgaccttgg tcgctccata cgaattcagg ccgcggccgg cgactgcgcg
      421 ggcaattgcc tgcggtcaga agcggaactg ggtgcccacg taaacggctt ggccgtcctt
      481 ctgtccgtca aaggcctgaa gcacgcggtc gcgctcctgc gaataccgta cgccggccgt
      541 cacattgaga ttgccggtca ggcgatagga gccgcccaca tccaccgtct gctcgccatt
      601 tccttccagc gtgcgcgggg cgcggccggt cttctcgcgc tcgtcgaacg cgatccgggc
      661 gttgaggcgc gaaggttcgt cggctccgcc cacacccgag cgcagattga agctcgaaag
      721 atcgggcccg tccacgcggg gcttgtcgcc ggccatggtg aagctctgga acccgcgcga
      781 aacgccaagg ctgtaggccg tcggcgcgat ccgcagtgcc gagagcgcgg cctggttgtc
      841 gcgcgaaggg gcctggacaa ggatcgcccg tccgcccacc gcgtcgaccc gcaccgccac
      901 ggtcaccgaa cggtcggggc gggtggcgct gcctgcgggc gtgaagtgga acgtttgtcc
      961 gcccaccagt ccgggcacgg tgatggcgcg ggcaaggcgg ggatcgacct gggccggcat
     1021 gaaattggcc atgctcgaat gggcgtcgag ccccggatcc tcggcgctgc cgaagtcgct
     1081 cgaaaaggcc agcacggcgc tgggcagggc gagcaggcac accgcacccg ccagcagcag
     1141 ggccgggctt cccgctccca tctgtccgcg cgtgatcgcc accatcgcca aaatccgctt
     1201 ccctctgccc tgcagcatag tctgccaagg tgaacccggg cataaccggg acccgccgga
     1261 atcgctccgg cgaagatgat tttacgccag atgcgcggcg attgccatgg tctcgcgtgt
     1321 tcagccgagg tcaacctctc ccggggcagt tccgccttgc gcctcaacgc cccttgtcgc
     1381 tcccggtccg gcggctatag aggctcgacc ggaatgccgt gcatgacggg taaccggccc
     1441 tcccgggcaa cgacgacagg aatgtagcga cgatgactgc ctcccgcatt tccggcacca
     1501 gcctttccgg tcgaaccgtt gcccgctgcg tgcgcagtct cggcacggcc gggctggcct
     1561 tcgtcctggc cggcggcctc gccggttgcg cccacaagga acgtcccaag gccgatctcg
     1621 cggcttcgcg ggtgacgacc atcggcgtca attcctatct gtggcgcgcc tcgctcgatg
     1681 cgctctcgtt catgccgctc gtccagaccg acagcagcgg cggcgtcatc gtgaccgact
     1741 ggtacgccaa tccccgcaat ccgggcgaac gcgtgaaggt caccgtgacc attctcgacc
     1801 aggaccttcg cgccgacgcc ctgcgcgtcg ccgccagccg ccaggtcgcg cagaacggca
     1861 cctgggtcga tgccccggtc caggccgcca cggtccagcg tctcgaagac gtgattctca
     1921 ccaaggcgcg cgaccttcgc cgccaggcga tttccggctg atactcgaca agtcggcctg
     1981 agcggctaga gggacggcca tgactggcac gccctctgac tctcggccct ccgacacccg
     2041 tttcgattcc gcttccgcag acctgcgctg gcagcgcatc tgggacgagg cgcaaacctt
     2101 ccgtgccgac gacaagagcg cgaagccccg ctcctacgtc ctcgagatgt tcccctatcc
     2161 ctcggggcgc atccacatcg gccacgtgcg caactacacc atgggcgacg tgctcgcccg
     2221 ggccaagcgg atgcagggct tcgaggtgct ccacccgatg ggctgggatg ccttcggcat
     2281 gcccgcggaa aacgccgcca tggaaaaggg cgtccacccc ggcggctgga cgcgcgacaa
     2341 cattgccaac atgaaggccc aattgaagcg cctcggcttc gcgctcgact ggtcgcgcga
     2401 gctcgcgact tgcgagccgg actactatgg ccacgaacag gccctgttcc tcgatctcta
     2461 tgcggcgggc ctcgtctatc gcaaggaatc ggaggtcaac tgggacccgg tcgatcatac
     2521 ggtcctcgcc aacgagcagg tgatcgacgg acgcggctgg cgctcgggcg cgctggtcga
     2581 acggcgcaag ctcaaccagt ggttcctcaa gatcaccgat ttcgccgagg aactgctcga
     2641 aggcctcgcc acgctcgaca agtggcccga gaaggtccgc ctcatgcagg agaactggat
     2701 cggcaagagc cagggcctgc aattcgcctt caccctgtcc aacggcgaaa gcctcgaggt
     2761 ctataccacc cggcccgata cgatcttcgg cgcgagcttc gtcgccgtcg ccgccgatca
     2821 cccggtggcg cggggcgttg cgggcgccaa tccggccgcg caggagttca tcgccctgtg
     2881 caagcagggc ggcaccaccg cggccgaact ggaaaccgcc gagaagctcg gcttcgatac
     2941 cggcctcacc gcgaagcatc ccttcaccgg cgaggccctg ccggtctaca tcgccaattt
     3001 cgtcctgatg gactacggca ccggcgcgat catggccgtg
//
LOCUS       DATNBV010000557         2622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1893705,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000557 DATNBV010000000
VERSION     DATNBV010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893705"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VN627_13190"
     CDS             complement(<1..358)
                     /locus_tag="VN627_13190"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HXD04252.1"
                     /translation="MRPAEASYAYEKVAFEKNRDAYPDGLPALPEIPGGRYFDQAFYD
                     LEMEHLWRKTWVVAGHVSQLPQEGSFITFERLGASVVIVRDKRGEVRAFHNVCRHRGA
                     PLVLQSSGRVPRFVCPY"
     gene            complement(953..>2622)
                     /locus_tag="VN627_13195"
     CDS             complement(953..>2622)
                     /locus_tag="VN627_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HXD04253.1"
                     /translation="AAAGFASQTGPRAENQDFGGVHVGSALERMRHGILAAVADGVSG
                     GKAGREAAELAVRALVEGFYAMPDTVGPARAMQAPLAAYNRWLHAQGRGETMANSATT
                     FTAMALRGRRAHLVHVGDSRAWRYSGGRLTCLTTDHVRPEPDLSHVLIRAIGIEPELR
                     LDHAQLELAEHDRLVLTTDGVHGVLSPARIGAILAENASAEATAEQLVDAALAAGGRD
                     NATAVVVDVVRLPAPDHDGILAGLADLPFVAPPAPGQWIDGFQVERVLSTGRHAVLLV
                     ATDGEDGARVVLKFPRDEVLSERALRLAFAREIMLGQRVASPFVLAAHPVRPERQTAL
                     YGVLPYLTGESMAERMGRGLPGLKGGIEAAIGLTRAAAALHRLEVVHRDIKPDNVMLL
                     PDGGLKLIDLGVARLPRVEDFRPDEIPGTPGFMAPEQFAGNAGDALTDQFALGVTLYR
                     WFTGKWPYGEQEAFQRPRFGRAVAPSQHRPEIPSWLDNAILTAIQPDPAARFGDVVEL
                     LRALEGGGAREVGPRRKLPLAARHPELFWQVTSAVLLAALIASLLLR"
BASE COUNT          431 a          917 c          885 g          389 t
ORIGIN      
        1 gataggggca cacgaagcgg gggacgcgcc cgctgctctg gagcaccagc ggcgccccgc
       61 ggtggcggca gacattgtgg aaggcccgaa cctcgcccct cttgtcgcgc acgatgacca
      121 cactggcgcc gagccgctcg aacgtgatga acgaaccttc ctgcggcaat tgcgagacat
      181 gcccggcaac cacccaggtc ttgcgccaga gatgctccat ctcgaggtcg tagaaggcct
      241 ggtcgaaata gcgcccgccc ggaatctcgg gcaaggcggg caggccgtcg ggataggcgt
      301 cccgattctt ctcgaacgcc actttctcgt atgcgtagct tgcttctgcc ggtcgcatgc
      361 caattctccc gctgccaatc cgcttgtgcc agcggcgctt gctgaaaatt tagtagcacg
      421 attgcgggcg gtcgagcagc gaatccagac acattgtaaa cccggcttgc ctttcaccaa
      481 cgatttcatt ggcgcgagca gagccaaggc agatcagccg gaccgtttgc cgacccaaga
      541 ccatggtctt cgtcggtagc ctgcgtgccg gctcctgcaa aaaggccctc gcccggcttg
      601 cgaccagcag agccgaggag gcgggtgccg aactgcggtt gctcggaatg tgggggagaa
      661 ccggcattcc gcgaccaaac aatgaaccct tgggggtgcc ggcgcgacca ttgggatcgc
      721 agggccgaag aatgcgagtg cactgcaaaa taaaagttgc gtgacgccct gcaaatgcaa
      781 tagcatagca ggcatcggga atgtgtgccc caaggatggg gcatagcgaa caggcccgaa
      841 atccaaccat cgcgtggcaa tggcgccgcc cgaatcgatc cagccggatc gttcgaggcg
      901 gcttttttgc gtgcgcgaag aacgaagggc tctggccgtg gctttccggt gttcagcgca
      961 gcagcagcga ggcgatcagc gccgcgagca gcactgcgct ggtcacctgc cagaacagct
     1021 cgggatggcg cgccgccagg ggcagtttgc gccgggggcc tacttcccgc gccccgccgc
     1081 cctccagcgc gcgcagcagt tcgaccacgt cgccgaagcg ggccgccggg tccggctgga
     1141 tcgcggtgag gatcgcgttg tcgagccacg aggggatttc cggccggtgc tgcgagggcg
     1201 ccacggcgcg gccgaaccgc gggcgctgga acgcctcctg ttcgccatag ggccacttgc
     1261 cggtgaacca gcggtagagc gtcaccccca gcgcgaactg atcggtcaga gcgtcgccgg
     1321 cgtttccggc gaactgctcg ggtgccatga acccgggcgt gccggggatc tcgtcgggcc
     1381 ggaagtcctc cacccggggc agccgcgcca cgccaaggtc gatcagcttc agcccgccgt
     1441 cgggcagcag catcacgttg tcgggcttga tgtcgcggtg gaccacttcc agccggtgca
     1501 gcgcggccgc ggcgcgggtc agcccgatcg ccgcctcgat tccccccttg agaccgggca
     1561 ggccgcgccc catccgctcg gccatgctct cgccggtcag gtacggcagc accccgtaaa
     1621 gcgcggtctg gcgctcgggg cggaccggat gagcggccag cacgaagggg ctcgccaccc
     1681 gctgccccag catgatttct cgggcaaagg cgagccgcag cgcgcgctcc gaaaggacct
     1741 cgtcgcgcgg gaatttcagc actacgcgcg cgccgtcctc cccgtcggtt gccaccagca
     1801 aaacggcatg gcgcccggtc gacagcacgc gttccacctg gaacccgtca atccattgtc
     1861 ccggcgccgg cggggccacg aagggcaggt cggccagccc ggcgaggatc ccgtcatggt
     1921 cgggcgcggg cagccgcacc acatcgacca cgaccgccgt cgcattgtca cggccgccgg
     1981 ccgccagggc cgcgtcgacc agctgctcgg ccgtggcctc ggcgctggcg ttctcggcca
     2041 ggatcgcgcc gatccgcgcc ggcgagagca ctccgtggac cccgtcggtg gtcagcacca
     2101 gccggtcatg ctcggccagc tccagctggg catgatcgag ccgcagttcc ggctcgatgc
     2161 cgattgcgcg gatcagcacg tggctgaggt cgggctccgg gcggacatga tcggtggtca
     2221 ggcaggtcag tcggccgccc gaatagcgcc aggcccggct gtcgccgaca tggaccaggt
     2281 gggcccgccg cccacgcagg gccatggcgg tgaaggtcgt ggccgaattg gccatggtct
     2341 cgccccggcc ctgggcatgg agccagcggt tgtatgcggc cagcggtgcc tgcatcgcac
     2401 gggccgggcc gaccgtgtcg ggcatggcgt agaacccctc gaccagcgcg cgcaccgcca
     2461 gttcggcagc ctcgcgcccg gccttcccgc ccgaaacgcc gtcggccacc gccgccagga
     2521 tcccgtggcg catccgctcg agcgcgctgc cgacgtgaac cccaccgaaa tcctggtttt
     2581 ccgcgcgcgg ccccgtctgg ctcgcaaagc ccgccgccgc ca
//
LOCUS       DATNBV010000558        11596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4425473,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000558 DATNBV010000000
VERSION     DATNBV010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11596
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4425473"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1874)
                     /gene="tkt"
                     /locus_tag="VN627_13200"
     CDS             complement(<1..1874)
                     /gene="tkt"
                     /locus_tag="VN627_13200"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXD04254.1"
                     /translation="MSIDPSRLGPMANAIRALSMDAVQAANSGHPGMPMGMADVATVL
                     FSQFLKFDPAEPKWSDRDRFVLSAGHGSMLIYSLLHLTGYASPTLEDIRKFRQIDSVC
                     AGHPENFLIEGVECTTGPLGQGFAMAVGMALAERHLNATFGDELVDHKTWVIAGDGCL
                     MEGINHEAVGLAGTLKLGRLNVLWDDNKITIDGDTSLSTSEDILARYAASGWHTTACD
                     GHDFADVARALAEAQADPRPSLVACRTVIGKGAPNKQGGHNVHGSPLGADEIAAARDY
                     LGWSAAPFEIPADILADWRSLGAKGAAARAEWEARATASPKGAELARRMAGDLPADTG
                     FAAYAAEIAASQPKVATRKSSEMALEALTVGVPEMVGGSADLTGSNNTKTKATGPLTA
                     ADFAGRYVYYGIREFGMAAAMNGMALHGGVIPYGGTFLIFSDYARNAIRMSALQRARA
                     IYVLTHDSIGLGEDGPTHQPVEQVMSLRMIPGLNVFRPADAMETAECWELALQAKDRP
                     SALILTRQNLPAVRSDTEMKSARGAYRLKAATAARKVVLLATGSEVELALKVAADLEG
                     KGIGADVVSMPSWELFDAQPAAYRADLLAADALKVSIEAGVTLGWQKYVGDGITIGLD
                     R"
     gene            2022..2228
                     /locus_tag="VN627_13205"
     CDS             2022..2228
                     /locus_tag="VN627_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04255.1"
                     /translation="MTGERTETALARIDAALARINAATSRVSARTGDLARKHDHLRSA
                     VAQTLHDLDLLIGEQEGAGQEVAV"
     gene            2225..2581
                     /locus_tag="VN627_13210"
     CDS             2225..2581
                     /locus_tag="VN627_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HXD04256.1"
                     /translation="MSNVNLHIGGRTFTVACEAGQEEHILGLGRLIDEKVRTLGPGAN
                     QNETRMLLFAAVVLADELHETRHGPGAPPPPPPPPPPPLPPEPVEDAQLAGRLEAIAG
                     ALEKCAAALETLADEA"
     gene            2952..3527
                     /locus_tag="VN627_13215"
     CDS             2952..3527
                     /locus_tag="VN627_13215"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HXD04257.1"
                     /translation="MTDKPTLRARLRAERRAHVAALPASIRALILLRPPAPVAARVPE
                     GSVVGLYHATAHEAPTLGYARWFYENGRHLALPWFASREAPMQFRAWTDPFDLTDLEP
                     GPWGQLQPRAEAPELVPATAFVPLIGFTADLHRLGQGGGHYDRWLAAHPDTVPIGLAW
                     DMQRLDSLPREDHDHPLAAVVTPTRIYEGEA"
     gene            3527..3742
                     /locus_tag="VN627_13220"
     CDS             3527..3742
                     /locus_tag="VN627_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HXD04258.1"
                     /translation="MRTEPTWRIPIGVLALFAALLVYGIVIARYVPGIIGTWPTLAQT
                     PVYTVLGLLWLLPMRRFLIWMETGRWG"
     gene            3797..4174
                     /locus_tag="VN627_13225"
     CDS             3797..4174
                     /locus_tag="VN627_13225"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HXD04259.1"
                     /translation="MISPVATVEAFLARLGEPGGFVTAVCDWFTPATVYENIGMTKTT
                     GIEEAAAFAEQFVTGSGSVAIRVENLAVATVGNTVLTERVDHLVDAAGKNTMSIPLMG
                     IFVVEDGKIAQWRDYFDTAPFRA"
     gene            complement(4242..4318)
                     /locus_tag="VN627_13230"
     tRNA            complement(4242..4318)
                     /locus_tag="VN627_13230"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4282..4284),aa:Asp,seq:gtc)
     gene            complement(4422..5321)
                     /locus_tag="VN627_13235"
     CDS             complement(4422..5321)
                     /locus_tag="VN627_13235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HXD04260.1"
                     /translation="MQIGCIYPQTEVESDPGAVKAFAQAAEGMGFAFIESYDHVVGAN
                     RPSPSGTQRPYNLDSPFHEPFVLLSYMAAITERIGFATGILILPQRQAALVAKQAACL
                     DVLSNGRFRMGIGTGWNEVEYEALGSDFATRGARMGEQVEFMRALWTQREVTFHGEYN
                     HIEEAGLWPLPVQRPIPVWFGGASDRPYFGEKAKMGVLRRIARLGDGWIQQTMPRDRT
                     IELIGQFHEFAREYGRDPAKLGVETRLETGSTPEDDWAATTAGWEGTGITHIAVNTML
                     GGLKGMDAHIKRLEAFRRVVPAT"
     gene            5480..5662
                     /locus_tag="VN627_13240"
     CDS             5480..5662
                     /locus_tag="VN627_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HXD04261.1"
                     /translation="MGEMTDKLKGAANSLTGKAKQAAGERQGDPDLAAQGRVQDAKGG
                     LQNLKGKVKGALGDKI"
     gene            complement(5678..6634)
                     /gene="thyA"
                     /locus_tag="VN627_13245"
     CDS             complement(5678..6634)
                     /gene="thyA"
                     /locus_tag="VN627_13245"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HXD04262.1"
                     /translation="MAPLSARSDSADHYEWQYLALMRRIWDEGCERVDRTGVGTRALF
                     GAELRFDLSECRVPLLTTKRVYWKTAAREFLWFLTGDTNIRALCAQGVEIWTDWPLAK
                     YRRESGEDISAEDFSARIVADAEFAARWGDLGPVYGKQWVDWPVYEPEGETGLFRRRA
                     QGVNQVAEVVRSLRENPGSRRHIVEGWNVAELDGMALPPCHKSYQFFVSGRRLSCLLY
                     QRSCDVALGLPFNLFGAALFTRMMAQQCDLEAGDLVWMGGDVHLYLNHADLVHEQLNR
                     LPAGNPRLELTRRPESIFDYAIGDFAVTGYEPQKAISAPVAV"
     gene            6700..8238
                     /locus_tag="VN627_13250"
     CDS             6700..8238
                     /locus_tag="VN627_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04263.1"
                     /translation="MHAQGSLPPASWRLAAALLVTLGLALLAFAAVRSGTYYDDEIFT
                     IRLLRLCPTPTELIACANSRDVHPPLGYLLDAALRGWLGSWAAVKLAKGLAMALAAGW
                     LAWSAGRALPRPAFILLTALLALDGTLVMWTTSLRWYGWFVPLFTVCLAQVLWGRLSA
                     LGTLALLTGAGLLLFHVNYLAVIAMPLLGLGWWRRFGAGLSHRERGLALALVGAGLLA
                     CLPQAAVLARVHLHYHGPQTGSPVMALVQTGLTIFPGTALFPLAPLSLLASGTFAFAA
                     MAAWRQRLRLPPGLLMMLAFGAAVMIASGLGYRHRNSLFLHVTALPLVASVLAAIARP
                     RWRAAAVAIVLAASLQGALNAARHVGTIKRSYNTPYPAMLDRIARWGKACPHYAVAHT
                     DIVLAELLRGRAVQTGLGATGSVVLAPGDCLIVVSGSAAASWPEETARWRETIAAAPA
                     RGVAVVRFSPEPGAALVGRLTGHPVEDHAATLRLLRLTGPATIPEFGSTAPLLPPPPH
                     PGQR"
     gene            8322..8398
                     /locus_tag="VN627_13255"
     tRNA            8322..8398
                     /locus_tag="VN627_13255"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8356..8358,aa:Pro,seq:cgg)
     gene            complement(8561..9175)
                     /locus_tag="VN627_13260"
     CDS             complement(8561..9175)
                     /locus_tag="VN627_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04264.1"
                     /translation="MNPLAGLLAPALAVLPVALSGSAEPAGDALSSPVELTPAAAPGA
                     EIEREEPGPWRSFDEAARALRAAEPARQVRIEQRVIIRIAPGAVRGPATIDARRLFSP
                     FPTMARAPRTIERRTAQCQPVGSIAGVQPDGSRLLLFMRDQRILSATLEKGCSARDYY
                     SGFLVERTSDGMLCAGRDKLLARSGANCGMGKLSQIVVVGDDDE"
     gene            9358..10797
                     /locus_tag="VN627_13265"
     CDS             9358..10797
                     /locus_tag="VN627_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXD04265.1"
                     /translation="MTEKPDFAEFLAAAAALLGPRGLTADPDLLAPWLTDWRGQFTGR
                     ALAMASPESTAQVAALVRLCGEHGVAIVPQGGNSGMCGGATPDAQGQSLLLSLRRMDA
                     VRRLDVEARRITCEAGMILENLHHAAEAEGLRFPLSLGGKGSATVGGLISTNAGGTQV
                     LRHGSMRALVLGIEAVLADGSVFSALTPLKKDNRGFDLKQLLIGSEGTLGIVTAATLT
                     LVPAVAERVVAWCAVPSLQVARRLLLHCDDALGAALEGFEVIPRASLAAVLGYLPTAR
                     APLAGDHAWHVMVEAVADSAGAADLRERMESTIAGAFAAGLVEDAVFSASEAQAEAFW
                     TLRDSVAPAERAKGPAVQHDISVPVEQMPDFVDHASAEIEARFPGVKAVAFGHLGDGN
                     VHFHAIAPAGSEHGAWQATTGKEISAVVNDMVTRWGGSISAEHGIGQLKIGELERLGD
                     PVALGLMRQVKQALDPRGLLNPGKLVRTA"
     gene            10939..>11596
                     /locus_tag="VN627_13270"
     CDS             10939..>11596
                     /locus_tag="VN627_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HXD04266.1"
                     /translation="MASAPQANLPVFYQDLMPLNSRDHTAWRSRTTDKAPWLAGFHAV
                     PLTVEEFPAAARNFPIIFSSGENPVPLALMGLNEGVNVFVDAEGKVAENIYIPAYVRR
                     YPFLLAKLSPDSEELSLCFDPTTDLVGEFDEGAPLFDGEQPSDQTKALLGFCEQFEQA
                     GLKTQAFVEELTKHKLLMDGEVAIQQEGVEAPFVYRGFQMVNQEAMRDLRGDVLRGWN
                     Q"
BASE COUNT         1825 a         4079 c         3803 g         1889 t
ORIGIN      
        1 cggtcgagcc cgatggtgat cccgtcgccg acatacttct gccagcccag agtcaccccg
       61 gcctcgatcg agaccttgag cgcatcggcg gccagcaggt cggcgcggta ggccgccggc
      121 tgggcgtcga acagttccca cgagggcatc gagacgacgt cggcaccgat gcccttgcct
      181 tcgaggtccg cggcaacctt gagcgccagt tcgacttccg agcccgtggc cagcagcacg
      241 accttgcggg ccgccgtggc agccttgagg cgataggcgc ccctggcgct cttcatctcg
      301 gtgtcggaac ggacggcggg caggttctgc cgggtgagga tcagcgccga ggggcggtcc
      361 ttcgcctgga gcgccagttc ccagcattcg gccgtttcca tcgcatcggc cggccggaac
      421 acattgagac ccgggatcat ccgcaggctc atgacctgct ccaccggctg gtgagtcggc
      481 ccgtcctcgc ccaggccgat gctgtcatgc gtcagcacgt agatcgcccg ggcgcgctga
      541 agggccgaca tgcggatcgc gttgcgggca tagtcggaga agatcaggaa ggtgccacca
      601 taggggatca cgcccccgtg cagcgccatg ccgttcatcg ccgcggccat gccgaactcg
      661 cggatgccgt aatagacata gcgcccggcg aaatcggccg cggtcagcgg gccagtggcc
      721 ttggtcttgg tgttgttcga accggtgagg tcggccgagc cgcccaccat ttccggcacg
      781 cccacggtca gcgcctcgag cgccatctcg ctcgacttgc gggtggcaac cttgggctgg
      841 cttgccgcga tctcggcggc ataggcggca aagccggtgt ccgccggcag gtcgccggcc
      901 atgcggcggg cgagttcggc ccccttgggc gaggccgtgg cacgtgcttc ccattccgcg
      961 cgggcggcgg cacccttggc gccgagcgag cgccagtcgg ccagaatgtc agcagggatc
     1021 tcgaacggag ccgcgctcca ccccagataa tcgcgagcgg cggcgatctc gtccgcgcca
     1081 agcgggctgc cgtggacgtt gtggccgccc tgcttgttgg gcgcaccctt gccgatcacc
     1141 gtgcggcagg cgaccagcga cgggcgcgga tcggcctggg cctcggccag cgcgcgggcg
     1201 acgtcggcaa agtcatgccc gtcgcacgca gtggtgtgcc accccgaggc ggcatagcgg
     1261 gcgaggatgt cttccgaagt cgagagcgag gtatcgccat cgatcgtgat cttgttatcg
     1321 tcccacagga cgttgagccg gcccaacttc agcgtgccgg ccagccccac cgcctcgtgg
     1381 ttgatgcctt ccatgaggca gccgtcgccg gcaatcaccc aagtcttgtg atcgaccagc
     1441 tcgtcgccga acgtggcgtt gaggtgccgt tcggccagcg ccatgccgac ggccatggca
     1501 aagccctgac ccagcgggcc agtcgtgcat tccacgcctt cgatcaggaa gttttccggg
     1561 tgcccggcgc agaccgaatc gatctggcgg aacttgcgga tgtcttccag cgtgggtgag
     1621 gcatagccgg tcagatgcag cagcgagtag atcagcatcg agccatggcc cgctgaaagc
     1681 acgaaccggt cacggtccga ccacttcggc tcggcggggt cgaacttgag gaactggctg
     1741 aacaggacgg tcgccacgtc ggccatgccc atcggcatgc cgggatggcc ggaattggcc
     1801 gcctgcaccg cgtccatcga gagggcgcga atggcgttgg ccatgggccc gaggcgggac
     1861 gggtcgatgc tcatgcgaat ttggctccag cgcagcggtc gggcgcgccc ctcgcgcgcg
     1921 tcgattcggg attgcggccc tttcgcggac gcactgcacc gcgtcaagtt taggcccggc
     1981 ggcgctttga ataaggaccg ttttgcgcta gcgaatcatt catgaccggc gaacgaaccg
     2041 aaacggcgct ggcgcggatc gacgccgccc ttgcccgcat caacgcagcc acctctcgcg
     2101 tgtcggcgcg gacgggcgat cttgcccgca agcacgatca cttgcgcagc gccgtggcgc
     2161 agacgttgca cgatctcgat ctcctgatcg gcgaacagga aggcgccggg caggaggtgg
     2221 ccgtatgagc aacgtcaacc tccacatcgg cggccgcacc ttcacggtgg cctgcgaagc
     2281 gggccaggaa gagcatatcc tgggcctggg ccgcctgatc gacgagaagg tccgcacgct
     2341 cggcccgggc gccaaccaga acgaaacacg gatgctgctc ttcgcggcgg tggtcctggc
     2401 cgacgaactg catgagaccc gccacggccc gggcgccccg ccgccgccgc ctccgccccc
     2461 acctccccca ctgccaccgg agccggtgga ggatgcacag ctcgccgggc gtctggaggc
     2521 catcgccggc gcgcttgaaa aatgcgccgc cgcccttgag accctggccg acgaagccta
     2581 agtgagacgg cgacgggtac tgcccggtgc gagcctttga aaatccctga ggctataagc
     2641 aatcctaggg ggctgtccct atcctggccc tggcccggaa tacacggtcc ccacctgacg
     2701 ttgaggcgtc agaggatttc ctggcaacgg cccatggtgg tcccgtcacc tattcgctga
     2761 gtgcgcaaag cccatccggg ctttgcttgc ggcaccggcg ccgggaaacg tcgcgccaga
     2821 tcctccctgt tttcgcgtgc agcacgcaaa tggggaggtg gcagcccgca gggctgacgg
     2881 aggggcaatt gcgccatccc gccaccaatg cccgccttgc ccccaaccaa acacccccta
     2941 tgaaagcccc gatgaccgac aagcccaccc tgcgcgcccg cctgcgggcc gagcgccgcg
     3001 cccatgtggc ggccctgccg gccagcatcc gcgcgcttat tctcctgcgc ccgccggctc
     3061 ccgttgcggc acgggtgccg gaaggatcgg tcgtcgggct ctatcatgcg accgcccacg
     3121 aggcgccgac cctcggctat gcccgctggt tctacgaaaa cggccgccac ctcgccctgc
     3181 cgtggttcgc ctcgcgcgag gcaccgatgc agttccgcgc gtggaccgat ccgttcgacc
     3241 tgaccgatct cgagcccgga ccctggggcc agctgcagcc gcgcgccgag gcgcccgaac
     3301 tcgtgccggc cacggcattc gttcccctca tcggcttcac cgccgacctc catcgcctcg
     3361 gccagggcgg cggccattat gatcgctggc ttgccgccca cccggacacc gtgccgatcg
     3421 ggcttgcctg ggatatgcag cggctcgatt cgctcccgcg cgaggatcac gaccacccgc
     3481 tcgccgccgt ggtcaccccc acccgcatct atgaaggaga ggcctgatgc gcaccgaacc
     3541 gacctggcgc atcccgatcg gcgtcctcgc cctcttcgcg gcgctgctgg tctacggcat
     3601 tgtgatcgcc cgctacgtac cgggcatcat cggcacgtgg ccgacgctgg cccagacgcc
     3661 ggtctacacc gtgctcggcc tgctctggct cctgcccatg cgccgcttcc tgatctggat
     3721 ggagaccggc cgctggggct gacttgcgcg ggcgctccgc tccgctaggc tggcccaaac
     3781 agcactggag agagccatga tcagccccgt cgccaccgtc gaagctttcc ttgcgcggct
     3841 gggcgagccg ggcggcttcg tcaccgcggt gtgtgactgg ttcacccccg cgaccgtcta
     3901 cgaaaacatc ggcatgacga agaccaccgg catcgaggaa gccgcggcct tcgccgaaca
     3961 gttcgtcacc ggctcgggct cggttgccat ccgcgtggaa aatctcgccg tggcgacggt
     4021 cggcaacact gtcctgaccg aacgggtcga tcacctcgtc gatgcggcgg gcaaaaacac
     4081 catgtcgatc ccgctgatgg gcattttcgt ggtggaagac ggcaagatcg cacagtggcg
     4141 ggactacttc gacacagcgc cgtttcgcgc ctaggccata cgcaaaaagg cgccggaacc
     4201 ggcgcctttt ttctgttcag cccttgctga aaatccccaa gtggcgcgag tgacggggct
     4261 cgaacccgcg acctccggcg tgacaggccg gcactctaac caactgagct acacccgctc
     4321 gccccttggg gagccgcgcc actaaggcag ggcgccaagg ctgtcaacag ccgatcgccg
     4381 gatcgcgcga ccatttgttc tcggtcgcga atccggcctg gtcaggtggc gggcaccacc
     4441 cggcggaacg cttcgagccg cttgatatgg gcatccatgc ccttcagtcc gccgagcatc
     4501 gtgttgaccg cgatatgggt aatgccggtg ccttcccacc ccgccgtggt ggccgcccaa
     4561 tcatcctccg gagtcgaccc cgtctccagc cgcgtctcga cgccgagctt ggccggatcg
     4621 cggccatatt cccgcgcgaa ctcgtgaaat tgcccgatca gttcgatcgt gcggtcgcgc
     4681 ggcatggtct gctggatcca gccatcgccc agccgcgcga tccgccgcag cacgcccatc
     4741 ttggcctttt cgccgaagta gggccggtcc gatgcgccgc cgaaccatac ggggatcggc
     4801 cgctggaccg gaagcggcca gagcccggct tcctcgatgt gattgtattc accgtggaag
     4861 gtcacctcac gctgggtcca cagggcgcgc atgaactcaa cctgctcgcc catccgggcc
     4921 ccgcgcgtgg cgaaatccga acccagcgcc tcgtattcca cctcgttcca acccgtgccg
     4981 atccccatgc ggaagcggcc gttggagagc acgtcgaggc aggccgcctg cttggcgacg
     5041 agcgcggcct gacgctgcgg caggatcagg atgccggtgg caaagccgat ccgctcggtg
     5101 atcgcggcca tgtaggagag caggacgaac ggttcatgga aggggctgtc gagattgtag
     5161 ggccgctggg tgccgctggg cgaaggccga ttggcgccga ctacgtggtc atagctctcg
     5221 ataaacgcaa aacccatgcc ctcagccgcc tgggcaaagg ccttcaccgc gcccggatcg
     5281 ctctcgacct ccgtctgcgg ataaatgcaa ccgatctgca tggcctgtac tccgtgtcat
     5341 caatgctgcc gctcatcgcg gcttccggtg gctaacaatc actctccttg ccgcaggtcc
     5401 accccccagg ccggaacagc gcaagcccgc acaagtttga tcgacgtgag cgcaccatct
     5461 gcgcaagagg agacaaacca tgggtgaaat gaccgacaag ctcaaaggcg ccgccaattc
     5521 gcttaccggc aaggccaagc aggccgccgg cgaacgccag ggcgatcccg accttgccgc
     5581 ccagggccgg gttcaggatg ccaagggcgg gctgcagaac ctcaagggca aggtgaaggg
     5641 cgcgctgggc gacaagatct gagccgctcc cgcgggttca gacggcgacc ggcgccgata
     5701 tcgctttctg cggttcgtag ccggtcaccg cgaaatcccc gattgcatag tcgaagatcg
     5761 attcgggccg gcgggtcagc tccagtcggg ggttgcccgc cggcagccga ttgagttgtt
     5821 cgtgcaccag gtcggcatgg ttcaggtaga ggtggacgtc gccgcccatc cacaccagat
     5881 cgcccgcttc aaggtcgcac tgctgcgcca tcatccgggt gaacaaggcg gccccgaaca
     5941 ggttgaacgg caggcccagc gccacatcgc agctgcgctg gtaaagcagg cacgacaggc
     6001 gccgccctga aacgaagaac tggtacgatt tgtggcacgg cggcagggcc atcccgtcca
     6061 gctcggccac attccatcct tcaacgatgt gccgccggct gcccgggttt tcccgcagcg
     6121 agcggactac ctcggctacc tggttcaccc cttgcgcgcg gcggcggaac aggcccgttt
     6181 cgccctccgg ctcatagacc ggccaatcga cccactgctt gccgtagacg ggacccagat
     6241 cgccccagcg cgccgcgaat tcggcatcgg cgacgatgcg ggccgaaaag tcttccgccg
     6301 agatgtcttc cccgctttcc cgccggtatt tcgccagcgg ccagtcggtc cagatctcca
     6361 ccccctgggc gcagagcgcg cggatattgg tatcgccggt caggaaccac aggaactcgc
     6421 gcgcggcggt cttccagtag acccgcttgg tggtcagcag cggcacccgg cattcggaca
     6481 ggtcgaagcg cagttcggcc ccgaacaagg cgcgggtgcc cacgcccgta cggtccaccc
     6541 gctcgcagcc ttcgtcccag atccggcgca tcagggcgag atactgccac tcgtagtgat
     6601 cggcggaatc ggaacgggcg gaaagcgggg ccatggcctc ctgcataagc ccggtgggca
     6661 gtttgccaag ccgccgcccg gccgatacga acagccgcga tgcacgcaca aggctccctg
     6721 ccgcctgcca gctggcgatt ggccgccgcc ctgctcgtaa ccctcgggct cgcgctgctc
     6781 gccttcgccg ctgtgcgcag cggcacctat tacgatgacg agatcttcac gattcgcctg
     6841 ctgcgcctgt gcccgacgcc aaccgagctg attgcctgcg ccaacagccg cgacgtccac
     6901 ccgccgctgg gttatctgct ggacgccgcg ctgcgcgggt ggctcggcag ctgggcggcg
     6961 gtgaaactgg ccaagggact ggccatggcg ctcgccgccg ggtggctggc gtggagcgcc
     7021 ggccgcgccc tgccgcgccc ggccttcatc ctgctcaccg ccctcctcgc gctcgacggg
     7081 acgctggtga tgtggaccac ctcgttgcgc tggtatggct ggttcgtgcc cctgttcacc
     7141 gtatgcctgg cccaagttct ctggggccgg ctctccgccc ttggcacgct cgcgctgctc
     7201 accggggccg gccttctcct tttccacgtc aactatcttg ccgtgatcgc catgccgctg
     7261 ctcggcctcg gctggtggag acggttcggg gcgggcctgt cgcaccggga gcggggcctt
     7321 gcccttgccc ttgtcggcgc cggcctgctc gcctgcctgc cgcaagccgc ggtgctggcc
     7381 cgggtacacc tgcattacca tggccctcag accggctcgc cggtcatggc cctggtacag
     7441 accggtttga cgatttttcc cggcacggcg ctgtttccgc tggccccgct ctcgctactg
     7501 gcctcgggca catttgcctt cgcagccatg gccgcctggc ggcagcgcct ccgcctgccg
     7561 ccggggctac tgatgatgct ggcgtttggt gcagcagtga tgatcgccag cgggcttggc
     7621 taccgccatc gcaacagcct gtttcttcac gtgaccgcct tgccgctggt cgcctcggtc
     7681 ctcgccgcca ttgcacgccc gcgctggcgg gctgccgccg tggcgatagt actcgccgcc
     7741 tcgcttcagg gcgccctcaa tgccgcccgc cacgtgggca cgatcaagcg ctcctacaac
     7801 acgccgtatc cggccatgct cgaccggatc gcacgctggg gcaaggcttg cccgcattat
     7861 gccgttgccc ataccgatat cgtactggcc gaactgctgc gaggtcgcgc cgtgcagacc
     7921 ggccttggtg cgacgggcag cgtagttctc gccccgggcg attgcctgat cgtggtcagc
     7981 ggttcagcgg cagcctcatg gccggaagaa actgcccggt ggcgcgagac catcgccgct
     8041 gcgcccgcgc gcggggtagc ggtggtgcgc ttctcccccg aacccggcgc cgcgctcgtc
     8101 ggccggctga ccggccaccc ggtggaggat catgccgcca cactgcgcct gctgcgcctc
     8161 accgggccgg ccaccatccc cgaattcggc agtaccgcgc cgctcttgcc cccgccgcca
     8221 cacccagggc aacgataatt gcaactcgca cttggcaagg ggggaatcgc cccttatagg
     8281 cgcgctcctg ccctgccccg gcgcaagcgg gggcatccgg tcggggagta gctcagcctg
     8341 gtagagcact gtcttcggga ggcaggggcc ggaggttcga atcctctctc cccgaccaca
     8401 tttttctgcc gattccggca tctataaggc cccgccagct cggcgcgacg agcttcgacc
     8461 gagcgggcgc aaggcgctcg gggttaaggc gaaggcccat ctccgctgcg gctctggtgg
     8521 gccgaaccta tccgatggtc acggcggctc ttccggtcac ctattcgtcg tcgtcaccga
     8581 cgaccacgat ctggctcagc ttgcccatgc cgcaattggc gcccgaccgc gccagaagct
     8641 tgtcccggcc cgcgcagagc atgccgtcgc tcgtccgttc gacgagaaac ccggaatagt
     8701 agtcacgcgc gctgcagccc ttttccagcg ttgcgctgag gatgcgctgg tcgcgcatga
     8761 agagcagcag gcggctgccg tcgggctgaa ccccggcgat ggagccgacc ggctggcatt
     8821 gggccgtgcg gcgctcgatc gtgcgcgggg cgcgggccat ggtggggaac ggcgaaaaca
     8881 gccggcgcgc atcaatcgtg gccggcccgc gcaccgcgcc aggggcgatg cggatgatca
     8941 cgcgctgctc gatccgcacc tgccgagccg gctcggccgc gcgcagggcg cgggcggcct
     9001 cgtcgaacga gcgccagggg ccgggctctt cgcgctcgat ctcagcgccc ggcgcggcgg
     9061 ccggggtcag ctcgactggc gatgagaggg catcgcccgc cggctccgca ctgcccgaca
     9121 gcgcgaccgg gagcacggca agtgccggtg caagcaggcc agcgagcggg ttcatggtcg
     9181 ggtcgggtct ccagccgggt tgcgggacag atcatacccg caccgaaatt cgcatctcat
     9241 ccgcattacc cgaaccggtt gaacagtcgc ttaactcacc ctagcgcgcc gcgaagcaaa
     9301 gccaagcgcc ggttgggcgg ctggcccgcc ggcacctgct atggcatagc ccgggccatg
     9361 accgagaagc ctgacttcgc cgaattcctc gccgccgccg ccgccctgct gggcccccgc
     9421 gggctgaccg ccgaccccga cctgctcgcc ccgtggctga ccgactggcg cgggcaattc
     9481 acgggccgtg ccctggccat ggcctccccc gaaagcacgg cgcaggtcgc cgcgctggtg
     9541 cggctgtgcg gcgaacatgg cgtggcaatc gttccacagg ggggcaattc cggcatgtgc
     9601 ggcggagcca cgcccgatgc gcagggccag tcgctgctgc tctcgctgcg gcgcatggac
     9661 gcggtgcgcc gcctcgacgt cgaagcccgg cggattacct gcgaggcggg gatgatcctg
     9721 gaaaatctcc accacgccgc cgaggccgag ggcctgcgct tcccgctttc gcttggcggc
     9781 aagggatcgg ccaccgtggg cgggctgata tcgaccaatg ccggcggcac ccaggtgctg
     9841 cgtcacgggt cgatgcgcgc gctggtgctg ggcatagagg cggtgctggc cgacggctcg
     9901 gtgttcagcg cgctgacccc gctaaagaag gacaatcgcg gattcgatct caagcaattg
     9961 ctgatcggct cggaagggac cttggggatc gtcaccgccg cgaccttgac cctggtacca
    10021 gcggtggccg aacgggtggt ggcatggtgc gcggtgccga gcctgcaggt ggcgcggcgg
    10081 ctgctgcttc actgcgacga tgccctgggg gcggcgctcg aaggcttcga ggtgattccc
    10141 cgcgccagcc tggccgcggt gctggggtac ttgccgaccg cccgtgcgcc gctcgccggc
    10201 gatcatgcct ggcatgtgat ggtggaagct gttgccgata gtgccggcgc cgcggatctg
    10261 cgcgaacgga tggaatcgac gattgccggt gccttcgcgg ccgggctggt cgaggacgcg
    10321 gtgttctccg ccagcgaggc tcaggccgaa gcgttctgga ccctgcgcga ttcggtggct
    10381 cccgcggaac gggccaaggg ccctgcggtg cagcacgaca tctcggtccc ggtggagcag
    10441 atgcccgatt tcgtcgacca tgcctcggcc gagatcgagg cgcggtttcc cggcgtgaag
    10501 gccgttgcct tcggtcacct gggcgacggc aatgtgcact tccatgccat tgcgccggcc
    10561 ggatcggaac atggcgcctg gcaggcgacg acggggaagg agatctcggc cgtggtcaac
    10621 gacatggtca cccgctgggg cgggtccatt tctgccgagc acggcatcgg ccagctcaag
    10681 attggcgagc ttgaacgact gggcgatccc gtggcgctgg gcctgatgcg ccaggtcaag
    10741 caagccctcg atcctcgggg gctgctcaat cccgggaagc tcgtccgaac ggcctgaact
    10801 cctgccgcat ccaccctgcc ctgcccttgc gcgggcatgc gccagcgctt aaagcctgcg
    10861 cccctgcccg gcggaggaaa acttccgccg gccagaccca attcggcccg cccagcgcgc
    10921 ccaaccggag agtgaccaat ggccagcgcg ccgcaagcca acctgcccgt gttctaccag
    10981 gacctgatgc ccctcaacag ccgcgaccac accgcctggc gcagccgcac gaccgacaag
    11041 gccccctggc tggccggctt ccacgccgtg ccgctcactg tcgaggaatt ccccgccgcc
    11101 gcgcgcaatt tcccgatcat cttctcctcg ggcgagaatc cggtgccgct ggcgctgatg
    11161 ggtctcaacg agggtgtgaa cgtgttcgtc gacgccgaag gcaaggttgc cgagaacatc
    11221 tacatcccgg cctacgtgcg ccgctatccg ttcctgctgg cgaagctgtc gcccgacagc
    11281 gaggagcttt cgctgtgctt cgacccgacg actgacctcg tcggcgagtt cgacgagggc
    11341 gcgcccctgt tcgacggcga acagccgagc gaccaaacca aggccctgct cggcttctgc
    11401 gagcagttcg agcaggccgg gctcaagacc caggccttcg tggaagagct gaccaagcac
    11461 aagctgctga tggacggcga agtggcgatc cagcaggaag gcgtcgaggc gccgttcgtc
    11521 tatcgcgggt tccagatggt caaccaggaa gccatgcgcg acctgcgcgg cgacgtgctg
    11581 cgcgggtgga accaga
//
LOCUS       DATNBV010000559         4176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3131721,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000559 DATNBV010000000
VERSION     DATNBV010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3131721"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VN627_13275"
     CDS             complement(<1..418)
                     /locus_tag="VN627_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HXD04267.1"
                     /translation="MRMTKRIAMALGVVGVAAGLGGVALAQQDMGMNGAMRAHDPFGS
                     ATVTRAEAQAKAAEMFARLDVNGDGKLDAADRMAAMSKRFDAMDANHDGVLSRQEFLD
                     AHQKMMGRMAGMQPGHEGGMMHHSGGMGRGMRMLGRM"
     gene            complement(631..2067)
                     /locus_tag="VN627_13280"
     CDS             complement(631..2067)
                     /locus_tag="VN627_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXD04268.1"
                     /translation="MTRHDSVRRTIKGSISSAILVALLATPAALVAQPSLKPAPVATA
                     SPAPAPVTSKPAPGSSPPPGSTSAVGADPVVTPPVTLPPVEVKEPVMHWPLADARQLL
                     ATINSVGAEGLAPADYTPAALKAAIAGGEGPELDEVASKAMAWLIEDRRDGRTPMAAR
                     VQWFAVDNDVEVNPTDAVMARALASHDIAGTLASLDPTYPDYAVLRQSLADTPRTNAK
                     ARALIRANMDRWRWFSRDLGEYYLMTNVPEFQLRLVRRDKIVRTYRTIVGKPGKTATP
                     QLAEKVQNVVFNPTWTVPQSIVVGEGLGAKYLKNPAKAAHDGYKVTTTADGMVIVVQQ
                     PGPKNSLGLVKIDMPNPHAIYLHDTPARALFNTPSRAYSHGCVRTERALELGMMLTIL
                     KAGKTPEEVTAISESGKYTKVPLTSPMPVYVGYFTMAKDINGELISWPDIYGRDAPVL
                     ASFAQPRQPKTGQRTSNEKVEAIDAPGA"
     gene            2248..3132
                     /locus_tag="VN627_13285"
     CDS             2248..3132
                     /locus_tag="VN627_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXD04269.1"
                     /translation="MIVAIGLFATMDALMKRAAIATGVYSALAARGMLAAAVLWPVWR
                     WRQGGRGWPAAEVLRLHALRGAVVAGMAASYFWGLVRTPLAEAIALSFIAPLIALFLA
                     AMFLGERLRRAAVAGSLVALAGVAVIASARISATSSTPAAGPGIAAILVSAVLYAWNL
                     VLQRRQAQLAGPLEVALFQNLFVTLALVAAVPVALLAGGSLPGLLVPARAALPDIAWA
                     ALLASASVMLLAWAYARAEAQALVPLEYTAFPWAALTGWLWFGEAMAMPTLAGLGLIL
                     LGVGIGARGAPQGQLPPA"
     gene            3279..>4176
                     /locus_tag="VN627_13290"
     CDS             3279..>4176
                     /locus_tag="VN627_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HXD04270.1"
                     /translation="MTQVGQDTLGTRSTMNVGGKDFAYYSLKKAAAKIGDVSRLPFSM
                     KVLLENLLRFEDGGFTVSADDVQAIADWQKNPVAPSREIQYRPARVLLQDFTGVPCVV
                     DLAAMRDAIAKLGGDTSKINPLVPVNLVIDHSVMVDEFGTPKAFEQNVEIEYQRNMER
                     YDFLKWGSKSLNNFYAVPPGTGICHQVNLEHIAQCVWTSTDQDGALVAYPDTCVGTDS
                     HTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFRFTGELQEGVTATDLVLTV
                     VQMLRKKGVVSKFVEFFGPGMDN"
BASE COUNT          621 a         1373 c         1420 g          762 t
ORIGIN      
        1 ccatccggcc gagcatgcgc atgcctcgtc ccatgccgcc ggaatgatgc atcattccgc
       61 cctcgtggcc gggctgcata ccggccatgc ggcccatcat cttctggtgc gcgtcgagga
      121 attcctgcct ggagagcacg ccgtcgtggt tggcatccat ggcatcgaag cgcttgctca
      181 tggccgccat gcgatcggcg gcatcgagct tgccatcgcc attgacgtca agccgggcga
      241 acatctcggc cgccttggcc tgggcttcgg cccgcgtaac cgtggcactg ccgaaggggt
      301 catgggcgcg catcgcgcca ttcatgccca tgtcctgctg ggccagggca acgccgccaa
      361 ggccggccgc gacaccaacg acgccgagcg ccatggcaat tcgcttcgtc atgcgcatcg
      421 tgaccatcct cttcgtccag gaaaggggcg aagccgacgg gaccgggtcg gggaagggag
      481 gggatgcgaa gtccggtccc gcggcttctg cctcatcata taggaggggt ttgtcgcggg
      541 attatgccag ccgaagcgtt tattgtcgcc atttgtcgcg ggtggccccg ggtgggaaag
      601 ccaaaccgct cccgccccgg ccggaccggg tcaggccccc ggcgcgtcga tcgcctcgac
      661 cttctcgttg ctggtgcgct ggccggtctt gggttgacgc ggctgggcga agctggcgag
      721 caccggcgca tcgcggccgt agatgtcggg ccaggagatc agctcaccgt tgatgtcctt
      781 ggccatggtg aaatagccga cgtaaaccgg catcgggctg gtcagcggga ccttggtgta
      841 cttgcccgat tcgctgatcg cggtgacttc ctccggtgtt ttgccggcct tgaggatggt
      901 cagcatcatg cccagctcaa gcgcgcgttc tgtccgcacg cagccatggc tgtaggcacg
      961 gctgggggtg ttgaagagcg cgcgggcggg ggtatcgtgc aggtagatcg cgtgaggatt
     1021 gggcatgtcg atcttgacga gaccaagcga gttcttgggc cccggctgct ggacgacgat
     1081 gaccatgcca tcggccgtgg tcgtcacctt gtagccgtca tgcgccgcct ttgccgggtt
     1141 cttgaggtac ttggcgccca ggccttcccc caccacgatt gactggggca cagtccaggt
     1201 cgggttgaac accacgttct ggaccttttc ggccagctgc ggtgtcgccg tcttgccggg
     1261 cttgccgacg atcgtgcgat aggtccgcac gatcttgtca cggcggacaa ggcgcagctg
     1321 gaactcgggc acgttggtca tcaggtaata ttcgcccaga tcccggctga accagcgcca
     1381 acggtccata ttggcgcgga tcagggcccg cgccttggca ttggtgcggg gggtatccgc
     1441 cagggactgc ctgagcacgg catagtcggg ataggtggga tcaagcgagg ccagggtgcc
     1501 ggcgatgtca tggctggcaa gagcccgggc catcaccgca tcggtcggat tgacctcgac
     1561 gtcgttgtcc accgcgaacc attggacccg ggcagccatc ggggtgcggc catcgcggcg
     1621 gtcctcgatc agccaggcca tggccttgct cgcaacttcg tcaagctcgg gcccttcgcc
     1681 gccggcgata gcggccttga gcgcggcggg cgtgtagtcc gccggggcga gcccttcggc
     1741 gccgacggaa ttgatcgtgg cgaggagttg ccgggcatcg gccagtggcc agtgcatcac
     1801 cggttccttg acctccacag gcgggagggt cacgggcggg gtgaccaccg ggtcggcgcc
     1861 gacggccgag gttgatccgg gcggcgggct ggagccgggg gccggcttgc tggttacggg
     1921 agccggagct ggcgaggctg tggcgacggg agccggcttg aggctgggct gggcgaccag
     1981 cgcggcgggc gtggcaagca gcgcgaccag aatcgcggaa gaaatcgaac ccttaatcgt
     2041 ccggcggacg ctgtcatgcc gtgtcacgtg attccccaaa agccgttcgc gcgggcgaag
     2101 ccgcttcgtg atgccctgtt ccgaggcaat catcaattgc ccgccctgcc ccatcttccc
     2161 tgtcgccaag ctgaatccac ccctatcctg acagcgatgc ccgtccgccg cgatccgttc
     2221 catcatccat ttgccccatt cttggcggtg atcgtcgcca tcggcctgtt cgcgaccatg
     2281 gatgcgctga tgaaacgggc ggcaattgcc actggcgtct actccgcctt ggctgcacga
     2341 ggcatgctcg ccgcggcggt gctgtggccg gtgtggcgct ggcggcaggg cgggcgaggg
     2401 tggccggcgg ccgaggtgct gcggctgcat gccctgcgcg gtgcggtcgt ggccggcatg
     2461 gcggcgagtt acttctgggg actggtgcgc acgccgctgg ccgaggccat cgccctgtcc
     2521 ttcatcgcgc cgctgatcgc gctgttcctc gcggccatgt ttctgggaga acgcctgcgc
     2581 cgcgctgccg tggctggatc gctggtggca ttggccggcg tcgctgtgat tgcatcggca
     2641 agaatcagcg cgaccagctc cacgcccgct gcaggtcccg gcatcgccgc catccttgtt
     2701 tcggcagtgc tctacgcgtg gaacctcgtg ctgcagcggc gccaggcgca gctggccggg
     2761 ccgttggaag tggcgctgtt ccagaacctc ttcgtcacgc tggccctcgt cgccgcggtg
     2821 ccggtggcgt tgctggccgg gggcagcctg cctggcctgc tggtccctgc ccgggccgcc
     2881 ctgcccgata ttgcctgggc cgcgctgctt gcctcggcct ccgtaatgct gctggcctgg
     2941 gcctatgccc gggccgaggc tcaggcactg gtgccgctcg agtatacggc ctttccctgg
     3001 gccgcgctca ccggctggct gtggttcggc gaggcgatgg ccatgccgac cctggcgggc
     3061 ctcggcctga tcctgctcgg cgtggggatt ggcgcgcgcg gtgcgcccca gggccagcta
     3121 ccccccgcct gacccgggat agcagtcgat ggtcggggag gccttgacgt tgcgcttgcg
     3181 aacgcgcatc ggcggcgcgc ccatttctca cgcctgcgca gccatgccgc actgcggcaa
     3241 gattcgcgca ggccagcctc cagggggact atagcctaat gacccaggtc ggacaggaca
     3301 cgctcggcac ccgttccacg atgaacgtcg gcggcaagga ctttgcctac tattcgctca
     3361 agaaggccgc tgcgaagatc ggcgacgtct cgcgcctgcc cttctcgatg aaggtgctgc
     3421 tggaaaacct gctccgcttc gaagatggcg gcttcaccgt ttcggcggac gacgtgcagg
     3481 ctattgccga ctggcagaag aacccggtcg cccccagccg cgagatccag taccgcccgg
     3541 cccgcgtcct cctgcaggat ttcaccggcg ttccctgcgt ggtcgacctt gccgccatgc
     3601 gcgatgccat tgccaagctc ggcggcgaca ccagcaagat caacccgctg gtcccggtca
     3661 acctcgtgat cgaccactcg gtcatggtcg acgagttcgg cacccccaag gcgttcgagc
     3721 agaacgtcga gatcgaatac cagcgcaaca tggagcgcta cgacttcctc aagtggggct
     3781 ccaagtcgct caacaacttc tacgcggtgc ccccggggac cggcatctgc caccaggtca
     3841 acctcgagca catcgcccag tgcgtgtgga ccagcaccga ccaggacggc gcgctcgtcg
     3901 cctatcccga tacgtgcgtc ggcaccgaca gccacacgac gatgatcaac ggcctcgggg
     3961 tgctcggctg gggcgtcggc gggatcgagg ccgaggcggc gatgctcggc cagccggtct
     4021 cgatgctgat ccccgaagtc gtcggcttcc gcttcaccgg cgagcttcag gaaggcgtga
     4081 ccgccaccga cctcgtgctg acagtcgtgc agatgctgcg caagaaggga gtcgtttcga
     4141 aattcgtcga attcttcggc ccgggcatgg acaaca
//
LOCUS       DATNBV010000560         3724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1894219,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000560 DATNBV010000000
VERSION     DATNBV010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894219"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..2050
                     /locus_tag="VN627_13295"
     CDS             125..2050
                     /locus_tag="VN627_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HXD04271.1"
                     /translation="MSETAQHPAPSPEAAAPVVGHGHSHGSLLGLAVGAIGVVFGDIG
                     TSPLYAMRDTFAGHHKLPLDALHLYGIVSLMFWSMMIIVTLKYVTVIMRADNKGEGGS
                     LALLALINQNISGKRWSAGIVLLGVFATSLFYGDSMITPAISVMSSIEGLAVYEPGLA
                     GAVVPMVVTILVGLFLIQSRGTAKVATFFGPIMLLYFLVIAVLGALSVAQHPQVIEAL
                     SPHHAVLLFAADPFRGFLAMGSAVLAVTGAEALYADMGHFGRSPIRVSWLVFVLPALV
                     LNYLGQASLLVRVGAPALESPFYLLAPEWFQWPLLVIASAAAVIASQAVITGAFSVTQ
                     QAIQLGFIPRMTIKYTSLNAGQIFIPVINWALMIAVILLVLMFQHSSNLTAAYGIAVT
                     GAMAIDNVLLAVVLFNLWNWKWWQSIPLLALFFSLDAAYLAANFTKIPDGGWVPLLMG
                     MTIFTLLTTWSKGRALMRQSMAEGTIPFDVFAKSAHSSAARVPGTAVFMASSDKGVPS
                     ALLHNIKHNKVLHERVVVLTISIQDVPYVDPAERSSVKQYGEGFFKVTLRFGFLEETD
                     VPSALSGVAMCGEPFEMMKTSFFLSRQTLIPSSKPGMAIWREKLFAWMLRNAASAMEF
                     FRLPTNRVVELGSQVEI"
     gene            2205..2576
                     /locus_tag="VN627_13300"
     CDS             2205..2576
                     /locus_tag="VN627_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXD04272.1"
                     /translation="MESEPAVRILSALAQEHRLAAFRMLVQAGRDGLAAGEIVSRLGI
                     APSSVSFHLAQLVGAGLATQTRHGRSIRYAPRYPTVGALVGYLIENCSGGEGCGDVPG
                     CGTAAADLPECVPSVSAKAGL"
     gene            complement(2737..3309)
                     /locus_tag="VN627_13305"
     CDS             complement(2737..3309)
                     /locus_tag="VN627_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HXD04273.1"
                     /translation="MAKFFGNLHLVLIVGLVLAIVVMVKFAGSAPVDANSVLRWLHVF
                     FGVLWIGLLYYLNFVQVPTMPKVPAELKKGVTGYIAPNVFFFFRYGALFTVLTGLGIA
                     FVDGYGHQALTFSGEGNVNLIGLGMWLALIMAFNVWFIIWPAQKKILGIVEATDEAKA
                     KAAPLALAASRTNLLLSLPMLYCMVSANLG"
     gene            complement(3467..>3724)
                     /locus_tag="VN627_13310"
     CDS             complement(3467..>3724)
                     /locus_tag="VN627_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HXD04274.1"
                     /translation="EAAFEGLDDDGWMILSRNWGFFFLVLAVLNEGLRHYLSFAGWLQ
                     AKLWLFLPLSFLFTFVHMPMLLRHGLAQSAEDEVVSHPPHE"
BASE COUNT          614 a         1196 c         1202 g          712 t
ORIGIN      
        1 agtgagccgc aagggccgtc ccgggcgctg caggcgtgtg gttgcggggg cgacagggcg
       61 ttgctaaggc gcggcacctt gccgcaaacg cggcgactcg ggggcaccgg gctggtcgtt
      121 tgcgatgagt gaaactgcac agcatcccgc accgtcgcca gaagcggcgg cgccagtcgt
      181 cggccatggc cattcccatg gctcgctgct cggccttgcc gtcggcgcta ttggcgtcgt
      241 cttcggcgac atcggcacca gcccgctcta cgccatgcgc gataccttcg ccgggcacca
      301 caagctcccg ctcgatgcac tccatctcta tggcatcgtc agcctgatgt tctggtcgat
      361 gatgatcatc gtgactctga aatacgtcac agtgatcatg cgggccgaca acaaggggga
      421 aggcggcagc ctcgccttgc tggcgctgat caaccagaac atctcgggca agcgctggtc
      481 ggccggcatc gtcctgctcg gggtcttcgc cacctcgctg ttctacggcg attcgatgat
      541 cacccccgcg atctcggtga tgtcgtcgat cgagggtctg gcggtttatg aacccggtct
      601 cgccggtgcg gtcgtgccca tggttgtgac gatcctcgtc ggcctgttcc tgatccagag
      661 ccgcggcacg gccaaggtcg ccaccttctt cggcccgatc atgctgctct atttcctcgt
      721 cattgcggtg ctgggcgccc tgtcggtggc gcagcacccg caggtgatcg aggcgctgag
      781 cccgcaccac gccgtcctgc tgttcgccgc cgatccgttc cgcgggttcc tcgcgatggg
      841 ctcggccgtg ctggcggtga ccggcgcgga agcgctctat gccgacatgg gccacttcgg
      901 gcgctcgcca atccgcgttt cgtggctggt cttcgtcctg ccggcgctgg ttctcaatta
      961 cctgggccag gcctcgctgc tggtccgcgt cggtgcgccg gcgctggaaa gcccgttcta
     1021 cctgctcgcg cccgagtggt tccagtggcc gctgctggtc attgccagcg ccgcggcggt
     1081 gattgcctcg caggccgtga tcaccggggc cttctcggtc acccagcagg cgatccagct
     1141 cggcttcatc ccgcgcatga cgatcaagta caccagcctc aacgccgggc agatcttcat
     1201 cccggtgatc aactgggcgc tgatgattgc cgtgatcctg ctggtactca tgttccagca
     1261 ctcatcgaac ctgaccgcgg cctacggcat cgccgtgacc ggcgcgatgg cgatcgacaa
     1321 cgtgctgctc gccgtcgtcc tgttcaacct gtggaactgg aagtggtggc agtcgatccc
     1381 gctgctggcg ctgttcttca gcctcgatgc cgcctacctc gcggccaact tcaccaagat
     1441 ccccgatggg ggctgggtgc cgctcctgat gggcatgacc atctttaccc tgctgaccac
     1501 atggtccaag gggcgcgcgc tgatgcgcca gtcgatggcc gaaggcacga tcccgttcga
     1561 cgtcttcgcc aagagcgccc attccagcgc cgcgcgcgtc cccggcacgg ccgtgttcat
     1621 ggcctcgagc gacaagggcg tgccctcggc cctgctgcac aacatcaagc acaacaaggt
     1681 gctgcacgaa cgcgtggtgg tcctgaccat atcgatccag gacgtgcctt atgtcgatcc
     1741 ggccgaacgc agttcggtca agcaatatgg cgagggcttc ttcaaggtga cccttcgctt
     1801 cggcttcctc gaggaaaccg acgtgcccag tgcgctgtcc ggcgtggcca tgtgcggcga
     1861 acccttcgag atgatgaaga caagcttctt cctctctcgg caaacgctga tcccatcgtc
     1921 caagccgggc atggcgatct ggcgcgagaa gctgttcgcg tggatgctgc gcaatgccgc
     1981 cagcgccatg gagttcttcc gcctgccgac caatcgcgtg gttgaactcg gcagccaggt
     2041 cgagatctga cttacgcaag ggcgcgccgg tgctctcccg gtcgccggcc tgtctgccca
     2101 cttcacctgt ccggctgctg gggataagta gccgacccgc agggctcgct gcttgctggc
     2161 ggaccatatt tcggtagttc tcgaattatc gaaatggagg tcgcatggag agcgagccag
     2221 ctgtccgcat tctctcagct ctggcccagg aacaccggct tgccgctttc cgcatgctgg
     2281 ttcaggctgg acgcgacggg ctggccgctg gcgagattgt cagccggctg ggcattgcgc
     2341 cctcctcggt cagttttcac cttgcgcagc tcgtcggcgc gggattggcg acgcaaacgc
     2401 ggcatgggcg ttcaatccgc tatgcgcccc gctatccgac ggtgggtgcg ctggtgggct
     2461 acttgatcga gaactgcagc ggaggcgagg gctgcggcga tgttccgggg tgcggcaccg
     2521 cggcggcaga cttgccggag tgcgtgccat cggtctcggc caaggccggc ctatagagtt
     2581 tcgcgcggcg gcgcggaagg tcgttcgcaa gagtgcgtgc cgggtcgcgg catgcttgcg
     2641 ggcgagaaca acgaaccctt ccacgggtcg caaacgggaa aggggcggag ccttgcggcg
     2701 ccgccccttt cttgtgtcgg tcagaaccgg cggtggttag ccgaggttgg cgctaaccat
     2761 gcagtagagc atcggcagcg acagcagcag gttggtgcgg ctggcggcga gggcgagcgg
     2821 ggcagccttg gccttggctt cgtcggtcgc ctcgacgatg ccgaggatct tcttctgggc
     2881 cggccagatg atgaaccaca cgttgaaggc catgatcagc gcgagccaca tgccgaggcc
     2941 gatcaggttc acattgcctt cgcccgagaa ggtcagcgcc tgatggccgt agccgtcgac
     3001 gaaggcaatg ccgaggccgg tgaggacggt gaacagcgcg ccgtagcgga agaagaagaa
     3061 cacgttcgga gcgatgtagc cggtcacgcc cttcttcagt tcggccggga ccttcggcat
     3121 ggtcggcacc tggacgaagt tcaggtaata gagcaggccg atccacagca cgccgaagaa
     3181 cacgtgcagc cagcgcagca ccgaattggc gtcgaccgga gccgagcctg cgaatttgac
     3241 cattaccacg atcgcgagca ccagcccgac gatcaggacg aggtgcaggt ttccaaagaa
     3301 cttggccatt gcgttccccc taattgcctt cgtttgttag catttccaaa cctccgccgc
     3361 gcggaggcat gggggagctt attgcgcgtt gatcgcagct tgtcacccgc tttcgcgcgc
     3421 tgaaccgccg ctatccagcg atcagtcgct cactcccaaa ggcttgtcac tcgtgcggag
     3481 ggtggctgac gacctcgtcc tccgcgctct gggcgaggcc gtggcgcagc agcatcggca
     3541 tgtgcacgaa ggtgaacagg aacgagagcg gcaggaacag ccacagcttg gcctgaagcc
     3601 agccggcgaa gctgagataa tggcgcagcc cttcgttgag cacggcaagg accaggaaga
     3661 agaaccccca gttgcgcgag aggatcatcc atccgtcgtc gtccagtccc tcgaacgcgg
     3721 cttc
//
LOCUS       DATNBV010000561         3641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3132856,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000561 DATNBV010000000
VERSION     DATNBV010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3132856"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..387
                     /locus_tag="VN627_13315"
     CDS             202..387
                     /locus_tag="VN627_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04275.1"
                     /translation="MRKLVLVAAATAGVLALSACKGEEAAPAATETAVESTEAAPADT
                     AVSTETATEASTEAAKM"
     gene            544..864
                     /locus_tag="VN627_13320"
     CDS             544..864
                     /locus_tag="VN627_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04276.1"
                     /translation="MRKAMLFLAAAVLGLGAAGCSQKTEEAARTAADAAASDAATNAA
                     KAGDAIAGAARDVGAKASEAASDAGDKLKAGGRQAADAANEAGDRTRHKAREASEEAT
                     EKAR"
     gene            complement(923..1330)
                     /gene="arsC"
                     /locus_tag="VN627_13325"
     CDS             complement(923..1330)
                     /gene="arsC"
                     /locus_tag="VN627_13325"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849768.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HXD04277.1"
                     /translation="MTDFPITIYHNPKCGTSRNTVAMVEAAGYAPTVVEYLKSGWTAP
                     QLAELVKAAGGTAQDLLRVKGTRAEELGLTAPGTPDDTIIAAMVADPILVNRPIVVTP
                     KGTRLCRPSELVLDLLDRKPAGFTKEDGEVVKI"
     gene            complement(1327..2037)
                     /locus_tag="VN627_13330"
     CDS             complement(1327..2037)
                     /locus_tag="VN627_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HXD04278.1"
                     /translation="MTPAVSTSRLFVAEALGTALLLAIVVGSGIMGARLAGGNDAIAL
                     LGNTIATGAGLVVLIHVFGPISGAHFNPVVTLAALFARQRTAGQALVMLAAQFLGGLI
                     GVWAAHLMFGEPVLQVSAKLRSGGAQVFAEAVATFGLIGTILGTQRARPEFTPVAVGL
                     YITSAYWFTASTSFANPAVTLARSLSETFAGIAPASVPGFIAGQVAGGLAGVVVFEWL
                     LAKQGQDLPLDEFSGTET"
     gene            complement(2172..2297)
                     /locus_tag="VN627_13335"
     CDS             complement(2172..2297)
                     /locus_tag="VN627_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04279.1"
                     /translation="MSFAGQPAAKRSTTMSTFTKIVLPVIIAAGTSGLMFTAALI"
     gene            2457..>3641
                     /gene="guaA"
                     /locus_tag="VN627_13340"
     CDS             2457..>3641
                     /gene="guaA"
                     /locus_tag="VN627_13340"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HXD04280.1"
                     /translation="MTIQPSDSILIVDFGSQVTQLIARRVREAGVYSEIAPFTTAAEA
                     FARMKPRGIILSGGPASVLDENGPRIPYEILDSGLPMLGICYGQQALMQQLGGEVLLG
                     DAGEFGRAFIEIEDRCVLFDGLWGEGEKHQVWMSHGDKVTRLAPGFRAVAASPGAPYA
                     VIANDEKRIYAMQFHPEVVHTPDGGKLLKNFVRHVCGLAGDWTMAEFRKTKIAEIREQ
                     VGSGKVICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGLMRLGEAEQVVGLFKGHYN
                     IPLIHKDVSALFLGGLAGVTDPEAKRKFIGKTFIDVFEEEARGIGGAEFLAQGTLYPD
                     VIESVSFTGGPSVTIKSHHNVGGLPERMNMKLVEPLRELFKDEVRELGRELGLPEIFV
                     GRH"
BASE COUNT          608 a         1164 c         1276 g          593 t
ORIGIN      
        1 gtgggcaagc tggtggaaaa ccgcgcggct gagagcgaat cggcctctgg agagcgacta
       61 gcgcttgcat cgcggggctc tcgccggtat ttgacgtgcg aagtctactc aagcggtagg
      121 ctttccgcac ggttttcgca tgcgccgaac aggtgtcagc ggggccgggc gtggtccact
      181 cgctgatcaa ggatttgttt catgcgcaag ctcgttctcg tcgctgccgc caccgccggc
      241 gttctcgctc tctcggcctg caagggtgaa gaagccgctc cggccgccac cgaaaccgcc
      301 gtcgagtcca ccgaagctgc tccggccgac accgctgttt cgaccgaaac ggccaccgaa
      361 gcttcgaccg aagccgccaa gatgtaatct tgccttcggg caaattgcac gaaggggcgc
      421 tcgagcatcg gctcgggcgc ccttttcttt tgccccgcgc gcccgcggcg gggcggggcg
      481 acggaaccgg gctgcgcaag cttcattgat cgggcattcg cctgaccaac ggagtttcgc
      541 gccatgcgca aggccatgct gttcctcgct gccgctgtcc tcggcctggg cgcggccggg
      601 tgttcgcaga agaccgagga agcggcccgg actgccgccg acgccgctgc ctcggatgcc
      661 gcgaccaatg cggcaaaggc cggcgatgcg atcgccggcg cggccaggga cgtgggcgcc
      721 aaggcttcgg aggctgcgtc ggacgccggt gacaagctca aggccggagg ccggcaggcg
      781 gccgatgcgg cgaacgaggc cggcgaccgg acgcgccaca aggcgcggga agcctctgag
      841 gaagcgaccg aaaaggcacg ctgaggctga agccactggc cgggattcca acccggccgg
      901 gagcctctgc gtccgggctg cctcagatct tcaccacctc gccgtcttcc ttggtgaagc
      961 cggcgggctt gcgatcgagg aggtcgagca ccagttcgga cgggcggcac aggcgggtgc
     1021 ccttgggcgt gaccacgatg gggcggttca cgaggatcgg atcggccacc atggcggcga
     1081 tgatcgtgtc gtccggggtg ccgggcgcgg tcaggccgag ttcctcggcc ctggtgccct
     1141 ttacccgcag caggtcctgc gcggtgccgc ccgccgcctt gaccagctcg gcgagctgcg
     1201 gcgcggtcca gcccgacttg aggtattcga ccacagtggg cgcgtagccg gcggcttcga
     1261 ccatggccac ggtgttgcgc gaggtgccgc acttggggtt gtggtagatg gtgatcggga
     1321 agtcggtcat gtctcggttc ctgaaaattc gtcgagcggg aggtcttgcc cctgtttcgc
     1381 caggagccat tcgaacacga cgacgccggc aagtccaccg gcgacctgcc cggcgatgaa
     1441 gccgggaacc gaggcggggg cgatgccggc gaacgtctcc gacaggctgc gcgcgagcgt
     1501 taccgccggg ttggcgaagg aggtgctggc ggtgaaccag taggcgctgg tgatgtagag
     1561 gccgacggcc acgggcgtga actccggcct ggcgcgctgg gtgcccagga tcgtgccgat
     1621 gaggccgaag gtcgcgaccg cctcggcaaa gacctgcgcg ccgccgctgc gcagcttggc
     1681 cgagacctgc aggaccggct cgccgaacat caggtgcgcg gcccacaccc cgatcaggcc
     1741 gccgaggaat tgggccgcga gcatgacgag agcctgcccg gcggttcgct ggcgggcgaa
     1801 gagggcggcc agggtgacga ccggattgaa gtgagccccg gagatcgggc cgaacacgtg
     1861 gatgagcacg accagcccgg cgccagtggc gattgtgttg ccgaggaggg cgatggcgtc
     1921 gtttccgccg gcaaggcgcg cgcccatgat gcccgagccg acgactatcg caagcaggag
     1981 cgcggtgccg agcgcttcgg cgacgaacaa gcggctggtt gatacggctg gtgtcattgc
     2041 ggcaggctcc cctgcgcgcg gactgcctca gcgagccgcg cgaggcaatg tcataattcg
     2101 gtcatcgaac gtgcaaatgc aaacggccgc cccgcgaggg gcggccgctc aggcttccga
     2161 tggggaagcg atcagatgag tgcggcggtg aacatcaggc cgctggtgcc agcggcgatg
     2221 atgaccggca gcacgatctt ggtgaaagtg ctcatggtgg tgctcctttt tgccgccggt
     2281 tgtccggcga aactcaatac ggcagttccg tattcgcatg tgcatctaag catgctcgtt
     2341 ttatgttgca aatgcgaaaa gatggagcac agttgcagtt cgtgcaacta ctctgctctg
     2401 gggagcaggc ccggcctccg ctggacagcg cgcgcgcgca tgcttatccg ccctgcatga
     2461 ccatccagcc ttcagactcg atcctgatcg tcgatttcgg cagccaggtg acccagctca
     2521 tcgcccgccg cgtccgcgaa gccggggtct attccgagat cgcgcccttc accacggcgg
     2581 cggaagcctt cgcgcggatg aagcccaggg gcatcatcct ctcgggcggc ccggcctcgg
     2641 tgctcgacga aaacggcccg cgcattccct acgagatcct cgacagcggc ctgcccatgc
     2701 ttggcatctg ctatggccag caggcgctca tgcagcagct tggcggcgag gtcctcctcg
     2761 gcgatgccgg cgagttcggc cgcgccttca tcgagatcga ggatcgctgc gtcctgttcg
     2821 acggcctgtg gggcgagggt gagaagcacc aggtatggat gagccacggt gacaaggtga
     2881 cccgtctggc ccccggcttc cgcgccgtcg ccgccagccc gggcgcgccc tatgcggtta
     2941 tcgcgaacga cgagaagcgc atctacgcga tgcagttcca ccccgaggtg gtccacaccc
     3001 ccgacggcgg caagctgctc aagaacttcg tgcgccacgt ctgcggcctt gccggcgact
     3061 ggaccatggc agaattccgc aagaccaaga tcgccgagat ccgcgagcag gtcggcagcg
     3121 gcaaggtgat ctgcggcctc tccggcgggg tggacagcgc ggtcgcggcc gtgctcatcc
     3181 acgaggcgat cggcgatcag ctgacctgcg ttttcgtgga ccatggcctg atgcggctgg
     3241 gcgaggccga gcaggtcgtc ggcctgttca agggccacta caatatcccg ctgatccaca
     3301 aggatgtctc ggcgctgttc cttggcggcc ttgccggggt caccgatccc gaggccaagc
     3361 gcaagttcat cggcaagacc ttcatcgacg tattcgagga agaggcgcgc gggatcggcg
     3421 gggccgaatt cctcgcccag ggcacgctct acccggacgt gatcgagagc gtcagcttca
     3481 ccggcgggcc ttcggtgacg atcaagagcc accataacgt cggcggactg cccgagcgga
     3541 tgaacatgaa gctcgtcgag ccgctgcgcg agctgttcaa ggacgaggtg cgcgagctgg
     3601 gccgcgagct gggcctgccg gagatcttcg tcggacgtca t
//
LOCUS       DATNBV010000562         2811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2225374,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000562 DATNBV010000000
VERSION     DATNBV010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225374"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VN627_13345"
     CDS             complement(<1..811)
                     /locus_tag="VN627_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HXD04281.1"
                     /translation="MKATQRDFPRQAESAARNARYFFFCGPDEAGVADAVDRVASLLE
                     DPGERIDFTGADLKRDPVRLGDEARSASLFGSSRHIVVRASGDEVAEATEILIGGTAP
                     SCPVLIAAPGATDKSKTAKLLADRKDALVVVFWPPDARSVAGTVRELADRAGLRIDTG
                     LAERIARAANLDTRIAASEVAKLAVFLDASPQAPRSADTKAFEAIGASTEEDGMAAIV
                     NAVLGGEPGRLGAELDRMRHLGLNPVAVLLAFERRTAQLAALAAKLGNGGGN"
     gene            complement(821..1336)
                     /gene="lptE"
                     /locus_tag="VN627_13350"
     CDS             complement(821..1336)
                     /gene="lptE"
                     /locus_tag="VN627_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HXD04282.1"
                     /translation="MNRYIAAVLLALAAAPLGGCGLQPLYAGGGQGQVARGLAAVDVP
                     PIEGKAGWLVRNALTDRLAAAGRTGGAPAYRLDVRLNDRLEGFGLLADDTIGRERRTL
                     RARYQLVDLASGKIVYDATGGADAGIDVVSSEYATIAAEQTALENLSKEVADRIVTAL
                     ALQLRSRDARR"
     gene            complement(1333..>2811)
                     /locus_tag="VN627_13355"
     CDS             complement(1333..>2811)
                     /locus_tag="VN627_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXD04283.1"
                     /translation="TKYGLPIRRVVASDESLADQPFNGEAEPGEGVLVNSYFINKLTV
                     AEARQAVIARAEAEGWGQGKTVWRLRDWGVSRQRYWGTPIPFIHCEVCGVVPVPKKAL
                     PVTLPDDVTFDVPGNPLDRHPTWKHVDCPHCGKPARRETDTLDTFVDSSWYFLRFASQ
                     PADAPFDPEEIKRWLPVEQYIGGIEHAILHLLYARFWTRALARIGKLEVKEPFASLFT
                     QGMVTHETYERRNPENGQPLFFSPTEVDRTGEGATLKADGLAVEIGRVIKMSKSKKNV
                     VDPDEIVAKYGADAIRWFMLSDSPPERDLPWSEAGIEGCARFVQRLWRLFGQFDAAAG
                     GEDEALDRRTHQTVAAVADDIAALSFNKAVARIYELTNATEKAAPSVSRSAAIRTLLL
                     LVAPMMPHLAEEAWAQMGGPGLVAEAAWPEVDPALLVDDEVTVAVQVNGKLRDTLTVA
                     KGSDKAALEALALASAKVQAILAGAAPKKVIVVPDRLVNVVA"
BASE COUNT          420 a          920 c         1036 g          435 t
ORIGIN      
        1 cgttgccgcc cccattgccg agcttcgcgg cgagcgcggc gagctgggcg gtgcggcgct
       61 cgaaggccag caggaccgcg accggattga gcccgagatg gcgcatccgg tcgagctcgg
      121 cgcccagccg gcccggctcc ccgccgagca cggcgttgac gatggcagcc atgccatcct
      181 cctcggtgct ggccccgatc gcctcgaagg ccttggtatc ggcgctgcgc ggggcctgcg
      241 ggctggcatc gagaaagacg gccagcttgg cgacttccga ggcggcgata cgggtatcga
      301 ggttggcggc gcgcgcgatc cgttcggcca gccccgtatc gatcctgagc cccgcccggt
      361 cggccagctc gcgcacggtg ccggcgacgc tgcgcgcatc gggcggccag aacacgacga
      421 cgagcgcgtc cttgcgatcg gcgaggagct tggcggtctt cgacttgtcg gtcgcccccg
      481 gcgcggcgat caggaccggg caggacgggg ccgtgccgcc gatcaggatc tcggtggctt
      541 cggccacctc gtcgcccgag gcgcgcacga cgatatggcg cgaggagccg aagagcgatg
      601 ccgagcgggc ctcgtcgccg aggcgcaccg gatcgcgctt gaggtcggcc cccgtgaagt
      661 cgatccgctc gcccggatct tccagcaggc tggccacgcg gtccaccgcg tcggcgactc
      721 cggcctcatc ggggccgcag aagaagaaat agcgtgcatt gcgggcggcg ctttccgcct
      781 gccgggggaa atcgcgctgg gtggccttca tggtgcggcc tcagcggcgc gcatcacgac
      841 tgcgcagctg gagcgcgagc gcggtgacta tccggtccgc cacttccttc gagaggtttt
      901 cgagcgcggt ctgctcggcg gcgatagttg catattcgga ggagaccacg tcgatccccg
      961 catcggctcc gccggtcgcg tcgtagacga tcttgcccga ggcgagatcg accagctggt
     1021 agcgcgcgcg cagggtgcgc cgctcgcggc cgatggtatc gtcagccagc aggccgaaac
     1081 cttccagccg atcgttgagg cgcacgtcga gccgataggc gggagcgccg ccggtgcgac
     1141 cggcggccgc gaggcgatcg gtcagggcat tgcggacgag ccagccggcc ttgccctcga
     1201 tcggcgggac gtcgactgcg gccagcccgc gcgccacctg gccctgcccg ccaccggcat
     1261 agagcggctg gagcccgcag ccgccaagcg gggcagcggc gagagcgagc aggacggcgg
     1321 caatatagcg gttcatgcga ccacgttaac gagccggtcc ggaacgacaa tcaccttctt
     1381 tggcgcagct ccggcgagaa tggcctgaac cttggcgctg gcgagcgcga gcgcctcgag
     1441 agccgccttg tcgctgccct tggcaacggt gagcgtatcg cgcagcttgc cgttgacctg
     1501 gaccgccacg gtcacctcgt catcgaccag cagggccgga tcgacctcgg gccaggcggc
     1561 ctcggccacc aggcctggtc cgcccatctg cgcccaggct tcctccgcga ggtgcggcat
     1621 catcggggcg accagcagca gcagggtgcg gatcgcggcc gagcggctga ccgagggcgc
     1681 ggccttttcg gtggcgttgg tcagctcgta gatgcgagcg acggccttgt tgaacgagag
     1741 cgcggcgata tcgtcggcga cggcggcgac agtctggtgg gtgcggcgat cgagcgcctc
     1801 gtcctcaccg ccagccgccg catcgaactg gccgaacagg cgccacaagc gctggacgaa
     1861 gcgggcacag ccctcgatgc cggcctcgga ccagggcagg tcgcgctcgg gcggcgaatc
     1921 ggacagcatg aaccagcgga tcgcgtcggc gccgtacttc gcgacgatct cgtccggatc
     1981 gaccacgttc ttcttcgact tcgacatctt gatgacgcgg ccgatctcga cggccaagcc
     2041 atcggccttg agggtcgcgc cttcgccggt gcggtccacc tcggtcgggg agaagaacag
     2101 cggctggccg ttttcgggat tgcggcgctc gtaggtctcg tgagtgacca tgccctgggt
     2161 gaacaggctg gcgaacggct ccttcacctc cagcttgccg atgcgggcca gggcgcgggt
     2221 ccagaagcgg gcgtagagga ggtggaggat cgcgtgttcg atgccgccga tatactgctc
     2281 cacgggcaac cagcgcttga tctcttcggg atcgaacggc gcatcggcgg gctggctggc
     2341 gaagcggagg aagtaccagg agctgtcgac gaaggtgtcg agcgtgtcgg tctcgcgccg
     2401 ggccggcttg ccgcagtggg ggcagtcgac gtgcttccag gtggggtggc ggtcgagcgg
     2461 gttgccgggg acgtcgaagg tgacgtcatc cgggagggta acgggcaggg ccttcttcgg
     2521 gacggggacg acgccgcaga cttcgcagtg gatgaagggg atcggggtgc cccagtagcg
     2581 ctggcggctg acgccccagt cgcgcaggcg ccagacggtc ttgccctgtc cccagccctc
     2641 ggcctcggcg cgggcgatga cggcttgcct ggcctcggcg acggtgagtt tgttgatgaa
     2701 atacgaattg acgagcacgc cttcgccggg ctccgcttcg ccattaaacg gttgatctgc
     2761 aaggctttca tccgaggcga cgacgcggcg gatggggagg ccgtatttgg t
//
LOCUS       DATNBV010000563         5895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4429713,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000563 DATNBV010000000
VERSION     DATNBV010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4429713"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VN627_13360"
     CDS             complement(<1..1158)
                     /locus_tag="VN627_13360"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXD04284.1"
                     /translation="MTFRLRTVSTTADGRRIVRDKDVSQDALTIGRAAENDIHLPDLA
                     VEPRHARLALQGQRVSVEATGSLGFVLDGQKLRSAAIDPRTGGELRFGSTRILVGEEE
                     GVVLLTVEPIAGEAEPEQDGMALAAVLPGKRRLAWILAGLILALFLALPVASYLMRAP
                     DVKQSHVVGDGAWTPGALSLAHHQLEGKCEACHVKPFQSVRNETCISCHKGVHDHADE
                     ARLAMARAEPGLGGKFLAKVAHTFGKEGAGACMDCHREHEGMTRMAPPAQQFCADCHG
                     SLKDRLGDTKLGNAADFATLHPQFAPAVVTNAAAGTVQRLSLDANPRENSGLAFPHKL
                     HLDPRGGVAKMAATLAADKGYGQNGLACADCHRPTEDGVRFKPIVMERDCEA"
     gene            complement(1169..2050)
                     /locus_tag="VN627_13365"
     CDS             complement(1169..2050)
                     /locus_tag="VN627_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04285.1"
                     /translation="MASTSAPRSTPGGAQTGRKVSADHEGFLRHRSFRWLKLAAVLCV
                     VAILAYALVDVQPHPNGGSWLGYTLGTIGAGLIVWLALLGVRKRAMTPGAWSLKAWTS
                     AHVYLGLSLIVVATLHTGLQFGWNVHTLAYVLMMLVIASGVWGIAVYASLPAALSANR
                     EELTREQMIEGIRAVDRQLHAAAQPLPHDQAELVQAALEQDLFAGGLATRLSGTWRNC
                     ATARAIDRFALAGPGDEAAEKVEKLLIRRKAQLDRLRRQMRLRAMLEVWLFIHVPATI
                     ALLAALAAHIISVFFYW"
     gene            complement(2050..4494)
                     /locus_tag="VN627_13370"
     CDS             complement(2050..4494)
                     /locus_tag="VN627_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXD04286.1"
                     /translation="MAERVRIAIVGSGPAGLSAAARAAELGLSHVLLEKTDHLSDTIY
                     KYQKGKHVMATPASLVLRSDFDFEAGKREAILGRWNEQAAAAGVNVRYNSEVVRITGE
                     KGAFELALGDGASVAAEAVILAIGTQGNPNRMRCTGGDLPHVQYQLDDPGEYVDEHIV
                     VVGTGDAGIENALGLAADAAQGNVVSILNRGADFARAKSANVKLLQEAAAAGRIAIRL
                     ETSPAEVREGELVLDTRDGQETIRCDRIIARMGSAAPRAFVEACGIEFTSPDREAFPV
                     LSPVFETTKPGIHVIGALAGYPLIKHCMNQGYDVVEFLNGNTTLKPADEPILADKFAP
                     LPGSRSVEDWLALFGSRIEIFRELSTLQLRELMLDSDVRAFAPGEAVFERNEPGSSLF
                     CIAEGAVGVGVNPADPSITVPIGEGSIFGEVGLISGRRRGATVRSTAPLIVVELSRNA
                     ALKLIATAPAAGRAVTRISIERQLLQIFGSGLTREDVAELVEGAEVKTVRAGEVVLAE
                     GDEGQDVFIIRRGSMIVQKELGGKLVFLSYVPAGSYIGEMALIDKAPRSATVKAAIRS
                     EVIRLPGEAFARLLEKKPDLLRKASADMDKRREVNAFIEGRKDSFGGAVDMYSATAQF
                     LIDNGIGEATDVLLIDEKLCVGCDNCEKACADSHEGLSRLNREAGKTYAHLHVPTSCR
                     HCEHPHCMADCPPNAIRRGPDGEVFIDDTCIGCGNCQRNCPYGVIRMDSVPPKKPSLL
                     SWLLFGSGPGPGEPSYKWRKKHASGEAPKKAIKCDMCSGIEGGPACVRACPTGAALRV
                     SPEAFLSVARLAEEAE"
     gene            4648..5583
                     /locus_tag="VN627_13375"
     CDS             4648..5583
                     /locus_tag="VN627_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXD04287.1"
                     /translation="MKPARILLAGAALVAGFAVAWTLTRTNSPTMPGSEVAASASGAA
                     DPLAALEQAARDNPQDIGAQRALGAGYFDAGRFDDAARAYEKAAGLAPQTAVVWSALG
                     EALVMASRQEPMPAPALDAFRKAAAIDPKDPRARYFLAVNKDLGGDHQGAIGDWLALL
                     ADTPPGAPWEADLRRTIEQVGKIHKIDVASRLAAVKQPAAPAGQGAGMGPPAPPGMPV
                     VAQGIPGPTAMDLKAAGSIPPSQQREMAEGMVARLEGKLKANPQNPDGWLMLIRSRAT
                     LGQPDKAGQALRDAVATNPGQAARLRQEAGVLGVK"
     gene            complement(5645..>5895)
                     /locus_tag="VN627_13380"
     CDS             complement(5645..>5895)
                     /locus_tag="VN627_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Pycsar system effector family protein"
                     /protein_id="HXD04288.1"
                     /translation="VAETDNLLFFGIYSKLSEEEFTARVIPLLTSDEAIWRAMLRDMH
                     QNGVVLARKKYRLLGYAYRAFLVGLVLTVIVFLGERVG"
BASE COUNT          952 a         1993 c         2022 g          928 t
ORIGIN      
        1 ggcctcgcaa tcgcgctcca tgacgatggg cttgaaccgc acgccgtcct cggtcgggcg
       61 gtggcaatcg gcacaggcca ggccgttctg gccatagccc ttgtccgccg ccagcgtcgc
      121 cgccatcttc gccacgccgc cgcgcggatc gaggtggagc ttgtgcggga aggccagtcc
      181 tgaattctcg cgcgggttgg cgtcgagcga caggcgctgg acggtgcccg cggccgcgtt
      241 tgtcaccacg gcgggagcga actgggggtg gagcgtggcg aaatctgcgg cgttgccgag
      301 cttggtgtcg cccaaccggt ccttgagcga tccgtggcaa tcggcgcaga actgctgcgc
      361 cggcggcgcc atcctggtca tgccctcgtg ctcgcgatgg caatccatgc aggcgcccgc
      421 gccctccttg ccgaaggtat gggccacctt cgccaggaac ttgccaccaa gccccggctc
      481 ggctcgggcc atggccagcc gcgcctcgtc ggcatggtcg tgcacgccct tgtggcagct
      541 gatgcaggtc tcgttgcgga cggactggaa cggcttgacg tggcaggcct cgcacttgcc
      601 ctcaagctgg tggtgggcaa ggctgagcgc gcccggagtc catgctccat cgcccacgac
      661 gtggctctgc ttcacgtcgg gcgcgcgcat caggtagctg gcgaccggca gggcgaggaa
      721 caacgccagg atcaggccgg caaggatcca cgccagccgc cgcttgcccg ggaggaccgc
      781 ggcgagggcc atgccgtcct gctccggctc ggcctcgccg gcgatgggct cgaccgtcag
      841 caggacgacg ccctcttcct cgccgacgag gatccgggtc gacccgaagc gcagttcgcc
      901 gccggtgcga ggatcgatcg cggcgctgcg cagcttctgc ccgtcgagca cgaagccaag
      961 cgagccggtg gcctcgacgc tgacgcgctg cccctgcaag gccaaccgcg catgacgggg
     1021 ctcgaccgcg agatcgggca ggtgaatgtc gttctcggcg gcgcggccga tggtcagcgc
     1081 atcctgcgag acgtccttgt cgcgaacgat tcggcggcca tcggcggtgg tggatacggt
     1141 gcggaggcgg aaagtcatgg gatgcccctc accagtagaa gaagacgctg atgatgtgcg
     1201 cggccagcgc cgcaagcagc gcgatggtcg ccggcacatg gatgaacagc cacacttcga
     1261 gcatggcgcg caggcgcatc tgccgccgca accggtccag ctgggctttc cggcggatca
     1321 gcagcttctc caccttctcg gccgcctcgt cgcccggccc ggcaagggcg aagcgatcaa
     1381 tcgcccgcgc cgtggcgcaa ttgcgccagg tccccgagag ccgcgtggcg agcccgccgg
     1441 cgaacaggtc ctgctccagc gcggcctgaa ccagttcggc ctggtcgtgg ggcaggggct
     1501 gggctgcggc gtgcagctgc cggtccaccg cccggatgcc ttcgatcatc tgttcgcgcg
     1561 tcaactcctc gcgattggcg ctgagcgcgg caggcaagct cgcataaacc gcgatgcccc
     1621 acacgcccga ggcgatgacc agcatcatca acacataggc gagcgtgtgg acgttccagc
     1681 cgaactggag gccggtgtgc agcgtcgcca cgacgatcag gcttaggccg agatagacat
     1741 gggccgaggt ccacgccttc aaggaccagg ctccgggcgt catcgcccgc ttgcgcacgc
     1801 cgagcagggc gagccagacg atcagcccgg ccccgatcgt gccaagggtg tagccgagcc
     1861 acgagccgcc gttaggatgc ggctgcacgt cgaccagcgc ataggcgagg atcgccacca
     1921 cgcacagcac ggccgccagt ttcagccagc ggaacgagcg gtggcgcagg aaaccttcat
     1981 ggtcggcgga aaccttgcgg ccggtctggg cgccgccggg cgtgctacgg ggggctgatg
     2041 tgctcgccat cactcggctt cctcggcaag gcgggcgacc gaaaggaatg cttccggcga
     2101 cacgcgcagg gccgccccgg tcgggcatgc ccgcacgcag gccggtccgc cctcaatccc
     2161 ggagcacatg tcgcacttga tcgccttctt aggcgcttcg cccgaggcat gcttcttgcg
     2221 ccacttgtag ctgggctcgc ccggcccggg tccgctgccg aacagcagcc aggagagcag
     2281 cgagggcttc tttgggggca ccgagtccat ccggatcacg ccataggggc agttgcgctg
     2341 gcaattgccg cagccgatgc aagtatcgtc gatgaacact tcgccgtccg gcccgcggcg
     2401 gatcgcgttg ggcgggcaat cggccatgca gtgcgggtgt tcgcaatgcc ggcagctcgt
     2461 cggcacgtgg aggtgcgcat aggtcttgcc ggcttcgcgg ttgaggcggg aaaggccttc
     2521 atggctgtcg gcgcaggcct tctcgcaatt gtcgcagccg acgcacagct tctcgtcgat
     2581 caggagaacg tcggtggcct cgccaatccc gttgtcgatc aggaactgcg ccgtggccga
     2641 atacatgtcg accgcgccgc cgaagctgtc cttgcgccct tcgatgaagg cgttgacctc
     2701 gcgccgtttg tccatgtcgg cgctggcctt gcgcagcagg tcgggtttct tctccagcag
     2761 tcgggcgaaa gcctcgcccg gcaggcggat tacttccgaa cggatcgccg ccttgaccgt
     2821 ggccgagcgc ggcgccttgt cgatcagcgc catctcgccg atgtaggacc cggcggggac
     2881 gtaggagagg aagaccagct ttccgccgag ctccttctgc acgatcatcg agccgcggcg
     2941 aatgatgaag acatcctggc cttcatcgcc ctcggccagc acgacctcgc cggcgcggac
     3001 cgtcttcacc tcggcgcctt cgaccagttc ggccacgtct tcgcgggtca ggcccgagcc
     3061 gaagatctgc agcagctggc gttcgatcga aatgcgggtg acggcgcgtc cggcggcggg
     3121 cgccgtggcg atcagcttga gggccgcgtt gcgcgaaagc tcgaccacga tcagcggggc
     3181 ggtggaccgg acggttgccc cgcgacggcg gccggaaatc aggcccactt cgccgaagat
     3241 cgaaccttcg ccgatgggca ccgtgatcga cggatcggcc ggattgaccc ccacccccac
     3301 ggcgccttcc gcgatgcaga acagcgagga gcccggctcg ttgcgctcaa acacggcctc
     3361 gccgggggcg aaggcgcgca cgtcgctgtc cagcatcagc tcgcgcagct ggagggtgga
     3421 cagttcgcgg aagatctcga tgcggctgcc gaacagggca agccagtcct cgaccgaacg
     3481 gctgccgggg aggggggcga atttgtccgc taggatcggc tcgtccgctg gcttcagcgt
     3541 ggtgttcccg ttgaggaact caaccacgtc atagccctgg ttcatgcagt gcttgatcag
     3601 cggatagccg gccagcgcgc cgatcacgtg aatccccggc ttggttgtct cgaataccgg
     3661 cgagagcacg gggaaagcct cgcggtcggg cgaggtgaac tcgattccgc aagcctcgac
     3721 gaaggcgcgc ggcgcggccg agcccatgcg ggcgatgatc cgatcgcagc ggatggtttc
     3781 ctgcccgtcg cgcgtatcta ataccagttc gccctcccgc acctcggcgg gcgaggtttc
     3841 gagccggatg gcgatccgcc cggcggcggc ggcttcctgc agcagcttga cattggccga
     3901 cttggcgcgg gcgaagtcgg ccccgcggtt gaggatcgag acgacgttgc cttgcgccgc
     3961 atcggccgca aggccaagcg cgttctcgat acccgcgtcg ccggtcccga ccacgacaat
     4021 atgttcgtcg acatattcgc cgggatcgtc gagctgatac tgcacgtggg gcaggtcgcc
     4081 gccggtgcag cgcatgcggt tggggttgcc ctgggtgccg atggcgagga tcaccgcctc
     4141 ggccgccacg ctggcgccat cgcccagcgc cagttcgaag gcccccttct caccggtgat
     4201 gcgcaccact tcagaattgt agcgcacgtt gaccccggcc gcggctgcct gctcgttcca
     4261 gcggccgaga atcgcctcgc gcttgccggc ctcgaaatcg aaatcggagc ggagcacgag
     4321 gctggcggga gtcgccatca cgtgcttgcc cttctggtac ttgtagatcg tatccgagag
     4381 gtggtcagtc ttttccagca gaacgtggct gaggcccagc tcagccgcgc gcgcggcggc
     4441 gctcaggccg gccgggcccg atcccacgat cgcaatgcgt acccgctctg ccaatttgcc
     4501 ccccacctgt tgtcgcgggc aatagccgcg caattgccaa ccggtaaccc ttgccgctaa
     4561 tcccgtaagc ggcgccaagg ctgcggcgaa ttactgccaa cgtgacggga tgcttatgaa
     4621 cgcgaagcgc agggagttac aagtgcgatg aagccggccc gaatccttct tgctggcgcg
     4681 gcgctggtcg ccggttttgc cgtcgcctgg accctaaccc gcacgaattc gcccacaatg
     4741 cccggcagcg aggtcgccgc aagcgcctcg ggcgcggccg atccgctggc cgcgctcgaa
     4801 caggcggcgc gtgacaatcc acaggatatc ggcgcgcaga gggcgctcgg cgcggggtat
     4861 ttcgatgcgg gccggttcga cgatgcggcg cgcgcttatg agaaggcggc cgggcttgcc
     4921 ccccaaactg cggtggtatg gtcggcgctg ggcgaggcgc tggtcatggc cagccggcag
     4981 gagccgatgc cggcccccgc gctcgacgcc ttccgcaagg ccgctgcgat cgatccgaag
     5041 gacccccggg cccgctattt cctggccgtg aacaaggacc tcggcggcga tcaccagggc
     5101 gccatcggcg actggctggc cctgctggcc gatacgccgc ctggcgcgcc gtgggaggca
     5161 gatctcaggc gcacgatcga gcaggtcggc aagatccaca agatcgacgt cgccagccgc
     5221 ctcgccgcgg tgaagcaacc ggcagcgccg gcggggcagg gggccgggat gggccctccg
     5281 gccccgcccg gcatgccggt ggtggcccag ggcatcccgg ggcccacggc gatggatctc
     5341 aaggccgccg gttcgatccc gcccagccag cagcgcgaga tggccgaagg tatggtcgcc
     5401 cggctcgagg gcaagctcaa ggctaatccc cagaatcccg acggctggct catgctgatc
     5461 cgcagccgcg ccacgctcgg ccagccggac aaggcgggcc aggcgctcag ggacgccgtc
     5521 gcaaccaatc cgggccaggc ggcgcggctc cgtcaggaag cgggggtgct gggggtgaag
     5581 tgagcgttcc acgccggatc tgctgagttt tacggcaacg tcattggcgg gagccgcgtc
     5641 gatctcagcc cacccgctcc cccaaaaaaa cgatcacggt cagcaccagg ccgaccagaa
     5701 acgcccggta ggcatagccc agaagccggt atttcttgcg ggcaagcacc acgccgttct
     5761 ggtgcatgtc gcgtagcatg gcccgccaga tcgcctcgtc gctggtgagc agcgggatga
     5821 cgcgggcagt gaattcctct tccgaaagct tcgaatagat gccgaagaac aggaggttgt
     5881 cagtctccgc gacct
//
LOCUS       DATNBV010000564         2669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3137730,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000564 DATNBV010000000
VERSION     DATNBV010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3137730"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="VN627_13385"
     CDS             complement(<1..1190)
                     /locus_tag="VN627_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXD04289.1"
                     /translation="MTSFARVAARTLLALPLLALAAPPLGAAPRPRVAAAVPAAKPYT
                     GPWLYRGSDVPQDKEWIFGELPNGLRYAVRRNGVPPGQVAIRVRVDVGSLNETESERG
                     FAHFLEHLVFRQSKYLADSEAIPAWQRLGATFGTDTNAETTPTGTTFKIDLPEATDAG
                     LDESMKFLSGMVAAPTLSDHNIRTDLPIVLAEMRERGGAASRVQDEMREVLYAGQPLA
                     TRAPIGTEQALNAARQEAVRAFHARWYRPENTVVVLAGDRDPAQLAALVKKWFGDWQG
                     QGPRVPAPSFGDPVAPPGALAPAGGGAPIGQAKVLVEPDQPRSITWATLRPWRQVNDT
                     VVYNQGLMMDQVAQAVINRRLEARARAGSSFLVAAVNQQDVSRSTDATFVAVTPLSDD
                     WRKAV"
     gene            1398..1970
                     /locus_tag="VN627_13390"
     CDS             1398..1970
                     /locus_tag="VN627_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HXD04290.1"
                     /translation="MFIETETTPNPATLKFLPGQEVMPAGTREFTSPEAAEASPLAQA
                     LFDLGEVTGVLFGRDFVSVTAGPGVSWPALKPQVVAMLLDHFVSGAPLFAGGSAGGIT
                     VAAEDEIADDPADADVIAQIQELIETRVRPAVANDGGDIVYRGFREGVVYLSMQGACS
                     GCPSSAATLKNGIESLLKHYVPEVSEVRAA"
     gene            1985..2614
                     /gene="tsaB"
                     /locus_tag="VN627_13395"
     CDS             1985..2614
                     /gene="tsaB"
                     /locus_tag="VN627_13395"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXD04291.1"
                     /translation="MDTLVIDAATEACSVALVRGGEITAGTWALLGRGHAEALVPMIA
                     ELPGKGRAGRIAVALGPGSFTGLRVGLAAARALAFAWGAELVGYPTLALIAAQARAEA
                     GARPVTVATIGGHGEWFVQDFTTEGLPDSSLASLKPAEAAARGGALLVAGSQAEALVA
                     LRGAGTALPLASDARAFPLLPPALVSTRTEPLYGRAPDARLPGGVLAAG"
BASE COUNT          366 a          879 c         1023 g          401 t
ORIGIN      
        1 acggccttgc gccaatcatc gctgagcggg gtgaccgcga cgaaggtggc gtcggtcgag
       61 cgggagacgt cctgctggtt gaccgcggcg acgaggaacg agctgcccgc gcgggcgcga
      121 gcttccagcc ggcggttgat cacggcctgg gcaacctggt ccatcatcag gccctgattg
      181 tagaccacgg tatcattgac ctgccgccat gggcgcagcg tggcccaggt gatcgagcgg
      241 ggctggtcag gctccaccag cactttcgcc tggccgatag gggcgccccc gcctgccggg
      301 gcaagagcac ccggcggggc gaccggatcg ccgaagctgg gcgctggcac gcgcggcccc
      361 tggccctgcc aatcgccgaa ccacttcttc accagcgcgg cgagctgggc cggatcgcga
      421 tcgccggcga gcacgacgac ggtgttttcc gggcggtacc agcgggcatg gaaggcgcgc
      481 acggcctcct gccgggcggc attgagcgcc tgctcggtgc cgatcggcgc gcgggtcgcg
      541 agcggctggc cagcatagag cacctcgcgc atttcgtcct gcacgcgcga ggcggcgccg
      601 ccgcgctcgc gcatttccgc caggacgatg ggcaggtcgg tgcggatgtt gtggtccgac
      661 agggtgggcg cggcgaccat gccggagagg aacttcatcg attcgtcgag gcccgcatcg
      721 gttgcctcgg gcaggtcgat cttgaacgtg gtcccggtcg gcgtggtttc ggcattggtg
      781 tcggtgccga aggtggcgcc gagccgctgc caggcgggga tggcttcgct atcggcaagg
      841 tacttcgact ggcggaagac gagatgctca aggaagtggg caaagccgcg ctcgctctcg
      901 gtctcgttca gcgagccgac atcgacccgg acacggatcg ccacctgccc cggcggcacg
      961 ccgttgcggc gcacggcata gcgtaggcca ttgggcagtt cgccgaagat ccattccttg
     1021 tcctgcggga cgtcgctccc gcggtagagc caggggccgg tgtacggctt ggccgccggc
     1081 acggcggcag cgacgcgcgg cctcggtgcg gcgccgagcg gcggcgcggc cagggcgagc
     1141 agcggaaggg caagcagggt gcgggcggcg acgcgggcaa acgatgtcat cgcgggaggt
     1201 tataggagcc ggggggatga agggccagtg aatagggcgg cggtggatca gccgtagtga
     1261 ctgaccgaac ctgccccacc gcccccgcct tcggcggcgg ccccgctggc aaacctgcgg
     1321 ttcgccgtct gcgacacccc cgtttcgggg aggaatggct tgacccctgc ccccgcgccc
     1381 ccatatcgcg cagcaccatg ttcatcgaaa ccgaaaccac gccgaatccg gccacgctca
     1441 agttcctgcc cgggcaggaa gtcatgcccg cgggcacgcg cgagttcacc tcgcccgagg
     1501 cggcggaggc ttccccgctg gcccaggcgc tgttcgacct tggcgaggtg accggcgtgc
     1561 tgttcggccg ggatttcgtc tcggttacgg ccgggccggg ggtgagctgg ccggcgctga
     1621 agccgcaggt cgtggccatg ctgctcgacc atttcgtttc gggcgcgccg ctgtttgccg
     1681 gcggaagcgc gggcgggatc accgttgccg ccgaggacga gatcgccgac gatccggccg
     1741 atgccgacgt gatcgcccag atccaggagc tgatcgaaac gcgggtgcgg ccggccgtgg
     1801 ccaacgacgg cggcgacatc gtctatcgcg gcttccgcga gggcgtggtc tacctgtcca
     1861 tgcagggcgc ctgttcgggc tgcccctcgt ctgccgccac gctgaagaac gggatcgagt
     1921 ccctgctcaa gcattacgtc cccgaagtca gcgaagtgcg cgccgcctga cagagggcgc
     1981 ggccttggat accctggtta tcgatgccgc gaccgaggcc tgctcggtgg cgctcgtgcg
     2041 cgggggtgaa atcaccgccg gaacctgggc gctgctcggg cggggccatg ccgaagcgct
     2101 ggtgccgatg attgccgaac tgccgggcaa gggccgcgcc gggcggatcg ccgtggcgct
     2161 ggggccgggc agcttcaccg gcctgcgcgt gggcctggcc gcggcgcggg ccctggcctt
     2221 tgcctggggt gccgaactgg tgggctatcc gacgctggcg ctaatcgcgg cgcaggcccg
     2281 ggccgaggcc ggcgcacggc cggtgaccgt ggcgacgatt ggcgggcatg gcgagtggtt
     2341 cgtccaggat tttacgactg agggcttgcc ggacagttcg ctcgcttccc tgaagcccgc
     2401 cgaggccgct gcgcgcgggg gggcgctgct ggtggcgggc agccaggccg aggcgctggt
     2461 ggcgctgcgc ggcgcgggca cggccctgcc gctggcgagc gacgcgcggg cattcccgct
     2521 gctcccccca gcgctggtga gtacccggac cgagccgctt tatggacgcg cgcccgatgc
     2581 gcggttgccg ggcggcgttc tggcggcggg ctgaccgcgg gctgaggggc ggggtgagca
     2641 agatgggcga cgacgtcgac cggatcatg
//
LOCUS       DATNBV010000565         5106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2229859,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000565 DATNBV010000000
VERSION     DATNBV010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229859"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VN627_13400"
     CDS             complement(<1..667)
                     /locus_tag="VN627_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXD04292.1"
                     /translation="MPARPQAGPGRAAGPLVGLRVVEFESIGPGPHCAMLLADLGADV
                     VRIGREGGNGWPNPIMDRGRASVEVDIRSDEGREFALETCDLADVVIEGMRPGVMERL
                     GLGPEVMLARNPRLVYGRMTGWGQEGPLARVAGHDINYIALAGALAPMGRPGEPAPVP
                     LNLVGDFGGGSMFLAFGIMAALWERERSGLGQVIDAAIVDGVSSMMTMFSGLSPRGFS
                     LDRA"
     gene            complement(667..1428)
                     /locus_tag="VN627_13405"
     CDS             complement(667..1428)
                     /locus_tag="VN627_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD04293.1"
                     /translation="MQISGLAAIVTGGASGLGGATAEMLARAGAKVTIFDMNEEAGAA
                     HAAQIGATFSKVDVSDDEAVAAAVAAAEERNGVARVLVNCAGIGPAIKTVSRDFTPHP
                     MAAFRKAIEVNLIGTFNVLSKVAARLAQAEDLGEERGVIVNTASAAAFDGQIGQAAYS
                     ASKGGVVGMTLPIARELAQHKVRVMTIAPGLFRTPLLASLPQAAQDSLATQVPHPRRL
                     GRPKEYAQMVESIIANPMLNGETIRLDGAIRMAPK"
     gene            complement(1482..2609)
                     /locus_tag="VN627_13410"
     CDS             complement(1482..2609)
                     /locus_tag="VN627_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016542544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD04294.1"
                     /translation="MQLSETQSAIRDVVRAFARERIAPASRQFEAAGGYPRELFEELA
                     ALGLMGMTAPEELGGAGADVVSYALALMEIAAADGALSTIISIQNSLIVSGLIRDGSP
                     AQRERFLPGLLAGRAIGAFALTEADAGSDASALRTRARRVDGGYRLDGAKQFITSGRT
                     AGLAIVFAVTDPAAGKKGISAFLVPTDRPGYSVDKVEHKLGQGASDTCALRFDDLFLE
                     EDLRLGEEGQGYRIALSNLEAGRIGIAAQAVGMAQAALELAVAYAKERRSMGKAIIEH
                     QAVGFRLADLAAALEAARQLVLHAASTRDAGLPCLAEASMAKLVASETAERVCSGAIQ
                     TLGGYGYLEEFGLAKIYRDVRVCQIYEGTSDIQRMVIARGL"
     gene            2834..3991
                     /locus_tag="VN627_13415"
     CDS             2834..3991
                     /locus_tag="VN627_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HXD04295.1"
                     /translation="MDRFPRGRAAIVGAATYGIGEAPGMRSNDLAACAVLDALRQANL
                     TLPEVDALFVCTADDALSGLGLSEYLGIKPGFTDNNRTGGSAFMSHAIVATLALEAGY
                     CDVAVIVHASNQRTSGRGLVSMRPPSIYEERYRPMTPLSSYALAASRHMHQYGTTREQ
                     LAEVAVSARAWANLNPEAFCYADGPLTIDEVLSSRMVSSPLTARDCCLIVDGAAAVVM
                     VRADRARDHAARPAYLLGGAAATWHADIAAMPDLTVTAAAESSRRAYAMAGVGPEDID
                     VVQLYDAFTINTILFLEDLGFCPKGEGGRFVSGGRIAPGGSLPVNTNGGGLSYCHPGM
                     YGLLAMVEAVRQLRGEGGARQIDGVELALAHGNGGTLSSQATVILGTEATL"
     gene            4049..4834
                     /locus_tag="VN627_13420"
     CDS             4049..4834
                     /locus_tag="VN627_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXD04296.1"
                     /translation="MTDPTLNPMSLDGRNILITGAGQGIGYGIAEMAIGLGANVAIVE
                     RNPETAAKAVETLGADRAAAYVGDVSDEAFVEATVAQAVDRFGGIHGLVNGAGITRPS
                     MIEKMTSDQWRAVIDVHLTGAFLFLAAVGRDMLRRARAGDSNPGSIVNILSDAGVQGT
                     IGQVNYAAAKGGLASMAMSAAREWGKYGIRANSVAFGMVETPMTEVIRGEKFRDTYLS
                     RIPLARWSLPAEAAAPVCFLLSDAASYITGQRLSANGGSQMNG"
     gene            4872..>5106
                     /locus_tag="VN627_13425"
     CDS             4872..>5106
                     /locus_tag="VN627_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXD04297.1"
                     /translation="MPSNLPADLDPRIVFELDGDIATITMDQPAKRNAVAPPMWDAIA
                     ALFDHCSKNPAVRVVVLTGRGEAFCSGTDLAEID"
BASE COUNT          831 a         1864 c         1616 g          795 t
ORIGIN      
        1 cggcgcggtc cagcgagaat ccccggggcg aaaggcccga gaacatcgtc atcatcgacg
       61 agacaccatc gacgatcgcc gcgtcgatca cctgccccag ccccgatcgc tcgcgctccc
      121 acagcgccgc catgatcccg aaggccagga acatcgatcc gccgccgaaa tcgcccacaa
      181 ggttcagcgg cacgggtgcc ggctcgcccg gccggcccat gggcgccaga gcgccggcca
      241 gcgcgatgta attgatgtcg tgcccggcga cccgggccag cggaccctcc tgcccccagc
      301 cggtcattcg tccatagacc agccgcggat tgcgcgcgag catgacctcc ggcccaagcc
      361 ccaggcgctc catcaccccg ggccgcatgc cctcgatcac cacgtcggca agatcgcagg
      421 tctccagggc gaattcacgg ccctcgtcgg aacggatgtc gacctcgacc gaggcgcggc
      481 cgcgatccat gatcggattg ggccagccat tgccgccctc tcggccgatc cgcaccacat
      541 cggcgcccag atcggccagc agcatggcgc agtgcgggcc ggggccgatc gattcgaatt
      601 cgaccacgcg cagcccgacc agcgggcctg ccgccctgcc cggccccgcc tgcggccgcg
      661 cgggcattac ttcggcgcca tccggatggc cccgtcgagc cggattgtct cgccgttcag
      721 catcggattg gcgatgatgc tctccaccat ctgggcatat tccttggggc ggccaagccg
      781 gcggggatgg ggcacctggg tggccagcga atcctgcgcg gcctggggca ggctggcgag
      841 tagcggcgtt ctgaacaggc ccggagcaat ggtcatgacc cgcaccttgt gctgcgccag
      901 ctcgcgcgcg atcggcaggg tcatgccgac cacgccgccc tttgaggccg aataggccgc
      961 ctgcccgatc tggccgtcga aggccgccgc actggcagtg ttgacgatca cgccgcgctc
     1021 ctcgcccaga tcctccgcct gggccagccg ggcggcgacc ttggacagga cattgaaggt
     1081 gccgatgagg ttcacctcga tggccttgcg gaaggccgcc atgggatgcg gggtgaaatc
     1141 tcggctcacc gtcttgatcg ccgggccgat accggcgcag ttcaccagca cgcgagcaac
     1201 gccattgcgc tcctcggccg cggcaacggc tgcggcgacg gcctcgtcat cagacacgtc
     1261 gaccttgctg aaagtcgccc cgatctgcgc cgcgtgggca gcgccggctt cctcgttcat
     1321 gtcgaagatg gtgaccttgg cgccggctcg cgccagcatc tccgcggtcg cgccgcccag
     1381 ccccgaagcg cccccggtca caatcgccgc cagtcccgaa atctgcattg tcgtcacctt
     1441 ccttcgtcgg ccgcgccgcc attatcggca gccacggcgt cttacagccc ccgcgcaatc
     1501 accattcgct ggatatccga cgtcccctcg tagatctggc aaaccctgac gtcgcggtag
     1561 atcttggcga ggccgaactc ctcgagatag ccgtagccac ccagcgtctg gatcgcgccg
     1621 gagcagaccc gctcagccgt ctccgaagcc accagcttgg ccatcgaggc ttcggccagg
     1681 cacggcagcc ccgcatcgcg cgtgctcgcc gcgtggagca caagctgacg cgcggcttcc
     1741 agcgcggcag ccagatcggc cagacggaag ccgaccgcct ggtgctcgat gatcgccttg
     1801 cccatcgagc gccgctcctt ggcataggcc accgccagtt ccagcgcggc ctgggccatg
     1861 ccgaccgcct gcgcggcaat gccgatgcgg ccggcctcca ggttggacag ggcgatgcga
     1921 tagccctgcc cttcctcgcc aaggcgcagg tcttcctcga ggaacaggtc gtcaaagcgc
     1981 agggcacagg tgtcggaagc gccctggccc agcttgtgct cgaccttgtc gaccgaatag
     2041 ccgggccggt cggtcggaac gaggaaggca ctgatgccct tcttccccgc cgccggatcg
     2101 gtcaccgcga acactatggc aaggcccgcc gtccgccccg acgtaatgaa ttgcttggcc
     2161 ccgtcgagcc ggtagccgcc gtcgacccgg cgcgcgcgcg tgcgcagcgc cgacgcgtcg
     2221 gaaccggcat cggcctcggt cagggcaaag gcgccgatgg cgcgcccggc cagcaggccg
     2281 ggcaggaacc gctcgcgctg ggccgggctg ccgtcgcgga tgagccccga gacgatcagg
     2341 ctgttctgga tcgagatgat ggtggacagg gccccgtcgg cagcggcgat ctccatcagc
     2401 gccagggcat aggaaaccac gtcggcgccg gctccgccga gttcctccgg cgcggtcatt
     2461 cccatcaggc ccagcgcggc gagttcctcg aacaactcgc gcggatagcc gccggcggcc
     2521 tcgaactggc ggctggcggg ggcgatccgc tcccgcgcga aagcgcgcac cacatcgcga
     2581 atcgcgctct gggtttcgct aagctgcatc gcggtgtgtc cttgggccat ggcgcgcctc
     2641 cgcgcggagg atggagagga attctaacaa ttgttcggtg atgtcaaacc ggcttggcaa
     2701 aaaactaacg aacagtagac agttggcctg ccgcgtgtat tcttgttctg gttcgccacg
     2761 ttccgaccgc gctggtccac gcgggtccag aaagttcggc cggacggcga agtccaaggc
     2821 aaggagcgta gaagtggatc gtttcccccg gggccgcgcc gccatagtcg gcgccgcaac
     2881 ctacggcatt ggtgaagcgc ccggcatgcg ctcgaatgac ctcgccgcct gtgcggtgct
     2941 cgacgcgctg aggcaggcca acctcacctt gccggaagtc gatgccctgt tcgtctgcac
     3001 ggccgatgac gcgctgtcgg gcctcggtct ttcggaatat ctcggaatca agcccggctt
     3061 cacggacaac aaccgaaccg gcggctcggc tttcatgagc cacgccatcg tcgcgaccct
     3121 ggcgcttgag gcaggctatt gcgacgtggc ggtgatcgtc cacgccagca accagcgcac
     3181 ctcggggcgc ggcctcgttt cgatgcgccc gccctcgatc tacgaggaac gctatcggcc
     3241 gatgaccccg ctgtcgtcct acgccctggc cgcctcgcgt cacatgcacc agtatggcac
     3301 cacgcgcgaa caactcgccg aagtcgcggt atccgcccgg gcctgggcca atctgaaccc
     3361 ggaagccttc tgctacgccg acggcccgct caccatcgac gaagtgctgt cctcgcgcat
     3421 ggtgtcgagc ccgcttaccg ctcgcgactg ctgcctgatc gtcgacggcg ccgccgccgt
     3481 ggtgatggtg cgggccgatc gcgccaggga ccatgccgcc cgtccggcct acctgctcgg
     3541 cggggccgcc gccacctggc atgccgatat cgccgccatg cccgatctga cggtcactgc
     3601 cgccgccgaa tccagccggc gcgcctatgc catggccggg gtcggcccgg aggacatcga
     3661 cgtcgtccag ctctacgacg ccttcaccat caacacgatc ctgttcctcg aggatctcgg
     3721 cttctgcccc aagggcgaag gaggccgatt cgtttcgggc ggccggatcg cccccggggg
     3781 gagcctgccg gtcaacacca acggcggcgg cctgtcctac tgccatcccg ggatgtacgg
     3841 cctgctggcc atggtcgagg ccgtgcgcca gctgcgcggc gaaggtggcg cgcgccagat
     3901 cgatggcgtc gaactcgcgc tggcccacgg caatggcggc accttgtcga gccaggccac
     3961 cgtcatcctc ggaaccgagg ccaccctctg acccctgacc tctgacccct gacctctcac
     4021 ttctccgcat ctgccaggag ctcgcaccat gaccgacccc acgctcaatc ccatgtcgct
     4081 cgacgggcgc aacatcctga tcaccggcgc tggccagggc atcggctatg ggatcgccga
     4141 aatggccatc ggcctcggcg ccaatgtcgc catcgtcgaa cgcaatcccg aaacggcggc
     4201 aaaggccgtc gaaaccctcg gggctgaccg cgccgccgcc tatgtcggcg acgtgtccga
     4261 tgaggccttc gtagaagcaa ccgtcgccca ggctgtagac cggttcggag ggatccacgg
     4321 actcgtcaat ggagccggga tcacccggcc gtccatgatc gagaaaatga ccagcgatca
     4381 gtggcgcgcg gtgatcgatg tccacctcac cggcgcgttc ctgttcctcg ccgccgtggg
     4441 ccgcgacatg ctccggcggg cgcgggcggg cgacagcaat cccggttcca tcgtcaacat
     4501 cctgtccgac gccggcgtcc aggggaccat tgggcaggtc aactacgccg cggcgaaggg
     4561 cggtctcgcc tccatggcga tgagcgcggc acgggaatgg ggcaagtacg gcatccgcgc
     4621 caattcggtt gccttcggca tggtcgaaac accgatgacc gaggtcatcc gcggcgagaa
     4681 gttccgcgat acctacctgt cgcgcattcc gctcgcccgc tggtcgctgc ccgcggaggc
     4741 cgccgcaccc gtttgcttcc tgttgtccga cgccgcctca tacatcaccg gccagcgcct
     4801 gtcggccaac ggcggcagcc agatgaacgg ctgatcgccg cccgccgccg atcgtcaaac
     4861 gagtaaacgc catgcccagc aaccttcccg ccgaccttga tccccgcatc gtgttcgaac
     4921 tcgacggcga cattgccacg atcacgatgg accagccggc caagcgcaac gccgtggcgc
     4981 caccgatgtg ggacgcgatc gccgcgctgt tcgatcactg ctcgaaaaac ccggccgtcc
     5041 gggtcgttgt cctgaccggc cggggcgagg ccttctgctc gggcaccgac ctggccgaga
     5101 tcgacc
//
LOCUS       DATNBV010000566         3443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3139013,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000566 DATNBV010000000
VERSION     DATNBV010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3139013"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="VN627_13430"
     CDS             complement(<1..1354)
                     /locus_tag="VN627_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HXD04298.1"
                     /translation="MALLILGVNIIAGFALGMISHGLSASEAAEKYITLAVGDALVAQ
                     VPALLLSIAAAVIVTRVSDSRDLAGQIGGQFANPATWLPVAVVLGGIGMIPAMPQTVF
                     LPAAGLAFWLYRKLKQRADRPEPVVDVLPPPDPQRIVLDDVSDQTLVTIELGYGVVHL
                     VDERRGSPLVSRVTGVRKQLSQMFGFIVPQFRVRDALEMPPHDYRVLLGGVPLGQATV
                     RAEKILAIDAGEARDNHSLAGEATTDPSFGCPALWIDPAQRDHAIAEGFLTVDASTVI
                     ATHLNQLLADRPQALLGPDEVRAILDAVKEHSAGLVETVYPAPLTLAAITRLLRTLVE
                     EGVSLGHPVPILQSLSQAVQVTLEHERLVDLVRADLGAMIVGRICGPHERLPVITLDA
                     RLEGAIVSGLQDPATGQPVIEPDLARTIGERIAELIAGRQSSGGPSACPPALIVQPRA
                     R"
     gene            1323..2021
                     /locus_tag="VN627_13435"
     CDS             1323..2021
                     /locus_tag="VN627_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXD04299.1"
                     /translation="MMLTPRISRATTEKQRKGIADYFTPTLVKMKEASAGSNGLLGGS
                     SITDADNYPNRMGQTGTKAMTIPRDTTGGPKEGGGKVKRMQETLQKKLEKSETMRRLA
                     RQVKMVDTTEGVRIDLVDDAQFAMFRLGTTVLTPEAAELLAAIGQTIAPEAGTLTIRG
                     HTDAVPYRPGELANNWSLSAGRAEATRQALMRNGIREDRFRRIEGVADRELLIPASPH
                     DPRNRRISIVVAPS"
     gene            complement(2031..3254)
                     /locus_tag="VN627_13440"
     CDS             complement(2031..3254)
                     /locus_tag="VN627_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXD04300.1"
                     /translation="MATIPDSATPGGQRLTPAQRRAVWAGFLGWTLDAFDFFLMVFML
                     KAIAAEFGVGVKDVSEALFLTLAARPLGALVFGRLADRVGRRPVLMGVILAFSVLSAL
                     SGAAGSLTQLLLVRALFGFAMGGEWGLGASLVMETVPAAARGRVSGLLQAGYPAGYLL
                     ASLAYFLLFDALGWRWMFVLGLAPALLVAFIRAGVEESPVFLARRHETPPPALRTLAA
                     HWKRGLYLVVLMTAFTTLSHGTQDMYPTFLQEQQGFPTKLTGTITLLMNVGAICGALV
                     FGALSHRLGRQRTIIAAALLALPVIPLWAYGNGMAQLAVGAMLMQFAVQGAWSMVPAW
                     LNELSPFAVRATFPGVVYQLGNLLSSRNVVFQADLAASRGGDYGYALAVVAGVTVAVL
                     VVWTAFGPQPADDLD"
BASE COUNT          631 a         1233 c         1154 g          425 t
ORIGIN      
        1 ggcgcgcgcg cggctggacg atcagcgccg gcgggcaggc cgatggcccc ccggagcttt
       61 ggcgcccggc gatcagctcg gcaatgcgct cgccgatggt gcgggccaga tcgggctcga
      121 tcaccggctg cccggtcgcc gggtcctgca ggcccgaaac gatggcccct tcgagccgcg
      181 cgtcgagcgt gatcaccggc aggcgctcat gcggcccgca aatccgcccg acgatcatcg
      241 cgccaaggtc ggcgcggacg agatcgacca gccgctcgtg ttccagcgtc acctgcacgg
      301 cctgggacag gctctgcaga atcggcaccg ggtggccgag cgacacgccc tcctccacca
      361 gggtgcgcag caggcgggtt atcgccgcga gggtcagcgg ggcgggatag accgtttcga
      421 cgaggccggc cgaatgctcc ttcaccgcgt cgaggatcgc gcggacctcg tcggggccga
      481 gcagggcctg cggccggtcg gccagcagct ggttgaggtg ggtggcgatc acggtcgagg
      541 cgtcgaccgt cagaaacccc tcggcgatgg cgtggtcgcg ctgggccgga tcgatccaca
      601 gcgccgggca gccgaagctg ggatcggtcg tggcctcgcc tgccagcgaa tggttgtcgc
      661 gcgcctcgcc ggcatcgatg gcgaggatct tctcggcgcg caccgtcgcc tgcccgagcg
      721 gcaccccgcc cagcagcacg cggtagtcat gcggcggcat ctccagcgca tcgcggacgc
      781 gaaactgcgg cacgatgaag ccaaacatct gggaaagctg cttgcgcacc ccggtaaccc
      841 gggaaaccag cggcgatccg cgccgctcgt cgaccaggtg aaccacgccg tagccgagct
      901 cgatggtcac cagggtttgg tcggatacgt cgtccagcac gatccgctgc ggatcgggcg
      961 gcggcaggac atcgaccacc ggctcgggcc ggtcggcgcg ctgcttgagc ttgcggtaaa
     1021 gccagaaagc cagcccggcg gcgggcagga agaccgtctg cggcatggcc gggatcatcc
     1081 cgatcccgcc caggaccacc gccaccggca gccaggttgc cggattggcg aactggccgc
     1141 cgatctgccc ggcaaggtcg cggctgtcgg acacgcgcgt gacgatcacg gcggcagcaa
     1201 tggacagcag cagcgcaggg acctgcgcca ccagcgcatc gcccaccgcc agcgtgatgt
     1261 acttctccgc cgcctcgctt gcgctcagcc cgtggctgat catccccagg gcgaaaccgg
     1321 cgatgatgtt gacgcccagg atcagcaggg ccacgaccga aaagcagcgc aagggcattg
     1381 ccgactattt cacgccgacc ctcgtcaaga tgaaggaggc gagcgcgggc tccaacggcc
     1441 tgctcggcgg ttcatcgatc accgatgccg acaactaccc caatcgcatg ggccagaccg
     1501 gcaccaaggc gatgacgatc ccgcgcgaca ccaccggagg ccccaaggaa ggcgggggca
     1561 aggtcaagcg catgcaggag accctgcaaa agaagctgga gaaatccgaa accatgcgcc
     1621 gtctcgcccg gcaggtgaaa atggtggaca ccaccgaggg cgtgcggatc gatctggtcg
     1681 acgatgccca gttcgccatg ttccggctcg gcaccaccgt gcttaccccg gaagccgcgg
     1741 aactgctcgc cgcgatcggc cagaccatcg cgcccgaagc cggcacgctg accatccgcg
     1801 gccacaccga cgccgtgccc tatcgcccgg gcgaactggc caacaactgg tcgctctccg
     1861 ccgggcgcgc cgaggccacg cggcaggcgc tgatgaggaa cggcatccgc gaagaccgct
     1921 tccgccgcat cgaaggcgtg gccgaccgcg agctgcttat ccccgccagc ccccacgacc
     1981 ctcgcaaccg gcgtatctcg atcgtggtgg ccccgagcta acccgccagg tcaatccaga
     2041 tcgtccgccg gctgcgggcc gaaggcggtc cacaccacca gcaccgccac ggtcaccccg
     2101 gccacaactg ccagcgcgta gccgtaatcc ccgccccggc tcgccgcgag atcggcctgg
     2161 aacaccacgt tgcgcgagga cagcaggttg cccagctggt agaccacgcc ggggaaggtc
     2221 gcccggaccg cgaagggcga cagctcgttg agccaggccg ggaccatcga ccaggccccc
     2281 tgcaccgcga actgcatgag catggccccc acggcgagct gagccatgcc attgccgtaa
     2341 gcccacagcg ggatcaccgg caaggccagc agggccgcgg caatgatcgt gcgctgccgg
     2401 cccagccggt gcgagagcgc gccgaacacc aatgccccgc agatcgcgcc cacgttcatg
     2461 agcagggtga tcgtgcccgt cagtttcgtc gggaagccct gctgctcctg caggaaggtc
     2521 gggtacatgt cctgggtgcc gtggctcagg gtggtgaagg cagtcatcag caccaccaaa
     2581 tagagcccgc gcttccagtg ggcggcgagg gtcctgagcg cgggcggcgg cgtttcgtgg
     2641 cggcgggcga ggaacaccgg gctctcttcc acgcccgcgc ggatgaaggc caccagcaag
     2701 gccggggcga ggcccagcac gaacatccag cgccagccga gcgcatcgaa caagaggaaa
     2761 taggccaggc tcgccagcag gtatccggcc ggatagccgg cctggagcaa gccggagacc
     2821 cgccctcgcg cggcggcggg gacggtttcc atgacaaggc tcgcccccag gccccactcg
     2881 ccgcccatgg cgaagccgaa caaggcacga accagcagca actgggtcag gctccccgct
     2941 gcgccggaca gggccgaaag caccgagaag gccaggatca cccccatcag caccggtcgc
     3001 cggcccaccc gatcggccag ccgcccgaac accagcgcgc ccagcggccg ggcggcaagc
     3061 gtgaggaaca gggcttcgga cacgtccttc acccccacgc cgaactccgc ggcaatggcc
     3121 ttcagcatga acaccatcag gaagaagtcg aaggcatcga gcgtccagcc gaggaacccg
     3181 gcccagactg cccggcgctg cgcgggggtt aggcgctggc ccccgggcgt tgcactatcc
     3241 gggattgtcg ccatgccacc tgcccctgct ttgccactgg cgaagcctgc aagccccccc
     3301 gacggaaccc gcagacgcca aaaggcccgg cggaatggag tccgccgggc cagttggtgc
     3361 cccgggagag gcattgagtc aaacttggtg cgtcaagctt agcgcagcag cgacagcacg
     3421 ttctgctggc tctgacctga ccc
//
LOCUS       DATNBV010000567         3246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4431790,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000567 DATNBV010000000
VERSION     DATNBV010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4431790"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VN627_13445"
     CDS             complement(<1..843)
                     /locus_tag="VN627_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXD04301.1"
                     /translation="MTADVIPLRPRPGAPSLAPLLALTAPGMNAVNAIILDRMQSEIP
                     LIPALAGHLISGGGKRMRPMLTIAGAALCDYQGTRHHKLAAAVEFIHTATLLHDDVVD
                     GSDMRRGKQTANIVYGNPATVLVGDFLFTRSFELMVEDGSLKVLKILSRASSIIAEGE
                     VDQLTAQRKVETSEEHYLQIIGAKTAALFAAATRIAAVVAERSEADEQAHDAYGRNLG
                     VAFQLVDDAIDYDSEASEMGKGKGDDFRDGKMTLPVILAYARGTDEERQFWQDAIAGF
                     RAEDE"
     gene            922..1230
                     /locus_tag="VN627_13450"
     CDS             922..1230
                     /locus_tag="VN627_13450"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HXD04302.1"
                     /translation="MAQDTTSTAADPQLAAFRASIDNIDAALIHMLAERFRITKAVGA
                     YKAEHDLPASDPGREDRQIARLRRLAEDAQLDPDFGEKFIRFIIDEVIRHHHQAKSAS
                     "
     gene            1425..2861
                     /locus_tag="VN627_13455"
     CDS             1425..2861
                     /locus_tag="VN627_13455"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXD04303.1"
                     /translation="MAIGFLGLAGASGTAFWVQRINEQNSRAVAHTLEVESRINAFNS
                     ANERAETARRGYLLAPGAAFRLRMQGAQQLRDDLLERIDEITRDNPAQQERLHDLRQM
                     VMELDTLQDQTISGVVGVRERLAQNFGEDGAVLLTRRIRDKAREMLDEEEHLLTARQE
                     QQDTTIFRFNVLLVLTTVLVVALSAMTLTLIRRNLADLRASRNDLNQLNDELEDLVDL
                     RTEALRRANAEIQRFAYIVSHDLRAPLVNVMGFTAELETAREEIHRFAARLDVDQPGL
                     IDRATRSAIEEDLPEAIGFIRSSTRKMDRLINSILKLSREGRRTLTPEPVDVTALVRG
                     ILDSLQHRATEQGTTFTLDPLPALVTDRLGLEQILSNLLENAVKYLRPGVPGEIHVSG
                     HEEAARTVISVRDNGRGIDARDHQRVFELFRRSGPQDQPGEGIGLAHVRALAHRLGGT
                     VDLESTPGTGSTFHLSLPHSWQPGDSHA"
     gene            2854..>3246
                     /locus_tag="VN627_13460"
     CDS             2854..>3246
                     /locus_tag="VN627_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXD04304.1"
                     /translation="MPETHAPLPPGSAPVSIVMIEDDEGHARLILKNIRRAGITNPVH
                     HFLDGTTGLEFLINDARGPARNGPALVLLDLNLPDMSGIDILARLKGTEELRRTPVVV
                     LTTTDDKVEIQRCYDLGCNVYITKPVNYE"
BASE COUNT          577 a         1112 c         1053 g          504 t
ORIGIN      
        1 ctcgtcctcg gcgcggaagc cggcgatggc gtcctgccag aactggcgtt cttcatccgt
       61 gccgcgggca taggcgagaa tgaccggcaa ggtcatcttg ccgtcgcgga aatcgtcgcc
      121 cttgcccttg cccatttccg aggcctcgga atcatagtcg atcgcatcgt cgaccagctg
      181 gaaggcgacg ccgagattgc ggccataggc atcgtgcgcc tgctcatcgg cttcggagcg
      241 ttcggcgacg acggcggcga tgcgggtggc ggcggcgaac agggcggcgg ttttcgcgcc
      301 gatgatctgg agatagtgct cctcgctcgt ctccaccttg cgctgggcgg tgagctgatc
      361 cacttcgccc tcggcgatga tcgagctcgc gcgcgagagg atcttgagga ccttgaggct
      421 gccatcctcg accatcagct cgaacgagcg ggtgaacagg aaatcgccga ccagcacggt
      481 cgccggattg ccatagacga tgttggcggt ctgcttgccg cggcgcatgt cggagccgtc
      541 aaccacgtca tcgtgcaaca gggtcgcggt gtggatgaat tcgacggcgg cggcgagctt
      601 gtggtggcgg gtgccctggt agtcgcacag ggccgcgccg gcgatggtca gcatcggccg
      661 catgcgcttg ccgccgccgg agatgaggtg accggcgagc gcggggatca gggggatctc
      721 gctctgcatc cgatcgagga tgatcgcgtt cacggcgttc atgccgggcg cggtcagcgc
      781 caggagggga gccagcgagg gtgcgcccgg gcggggccgc agggggatca cgtcggcagt
      841 catagcgccg ttcccttggg cccgccctga acggaaggca agcttggatt ccgggtcact
      901 ctcgtgctag cgcgctcggc gatggcacag gataccacct caaccgcggc cgatccgcag
      961 ctggccgcct tccgcgcaag catcgacaat atcgatgccg cgctgatcca catgctggcc
     1021 gagcgattcc ggatcaccaa ggccgtcggc gcctacaagg ccgagcacga cctgccggcg
     1081 tccgacccgg gccgggaaga tcgccagatc gcccgcctgc gccggctggc cgaggatgcc
     1141 cagcttgacc cggatttcgg cgagaagttc atccgcttca tcatcgacga ggtgatccgc
     1201 catcaccacc aggccaaatc ggcgagctga acccagccaa ctgaccttac tgtaaaaaat
     1261 ccgggaacct ccggcaccac caaccgttag cagcgggttc gggggcgcat tcggtcggga
     1321 gggaccaggg atgcgcggga tgggaatcgc gcgcgatcat gcaagccaaa agcccgagct
     1381 cgctcggcag ggacgatcgg accaaccggc agatcgcact gctgttggcc atcggattcc
     1441 ttggcttggc gggcgcgagc ggaaccgcct tctgggtcca gcggatcaac gaacagaatt
     1501 cgcgggccgt ggcgcatacg ctggaggtcg aatcccggat aaatgcgttc aattcggcca
     1561 acgagcgcgc ggaaaccgcg cggcgcggct atctgctggc gcccggcgcg gctttccggc
     1621 tgcgcatgca gggcgcccag caactgcgcg atgatctgct tgagcgcatc gacgagatca
     1681 cccgggacaa tccggcccag caggagcgcc tgcacgacct gcgccaaatg gtaatggagc
     1741 tggacaccct gcaggaccag acgatcagcg gggtggtggg cgtacgcgag aggctcgcgc
     1801 agaatttcgg cgaggatggc gcggtgctcc tcacccgccg gatccgcgac aaggcgcggg
     1861 agatgctcga cgaggaggag cacctgctga ccgcgcggca ggagcagcag gacacgacca
     1921 tcttccggtt caacgtgctg ctggtgctga ccacggtgct ggtggttgcc ctctcggcca
     1981 tgaccctcac cctgatccgc cgcaacctgg cggacctgcg cgcctcccgc aacgacctca
     2041 accaactgaa cgacgaactg gaggacctgg tcgacctgcg caccgaggct ttgcgccggg
     2101 ccaatgccga gatccagcgg tttgcctata tcgtcagcca cgacctgcgc gcgccgctgg
     2161 tcaacgtgat gggcttcacc gccgaactgg agacggcgcg cgaggagatc caccgcttcg
     2221 ccgcccggct cgacgtcgac cagccgggcc tgatcgaccg ggcgacgcga tcggcgatcg
     2281 aggaggacct gcccgaggcg atcggcttca tccgcagttc cacccgcaag atggaccggc
     2341 tgatcaactc gatcctcaag ctctcccgcg aggggcgccg caccctgacg cccgagccgg
     2401 tcgacgtgac cgccctagtg cgcggcattc tcgactcgct ccagcaccgg gccaccgaac
     2461 agggcaccac cttcaccctc gatccgctcc cggccctcgt cacggaccgt ctcgggctgg
     2521 agcagatcct ctccaacctc ctcgagaacg cggtcaaata cctgcggccc ggcgtacccg
     2581 gcgaaatcca tgtgagcggc catgaagagg ccgcgcgcac ggtcatctcg gtccgcgaca
     2641 acgggcgcgg gatcgatgcg agggaccacc agcgggtgtt cgagctgttc cgccgctccg
     2701 gcccccagga ccagccgggc gaaggcatcg gcctcgccca tgtgcgtgcc cttgcccatc
     2761 gtctcggcgg caccgtcgac ctcgaatcca cgcccggaac gggttccacc ttccacctca
     2821 gcctgccgca ttcctggcag cccggagaca gtcatgcctg agacccacgc cccactcccc
     2881 cccggctccg ctcccgtcag catcgtcatg atcgaggatg acgagggcca tgcccgcctg
     2941 atcctgaaga atatccgccg ggccgggatc accaatccgg tgcaccactt ccttgacggc
     3001 acaaccggcc tggagttcct gatcaacgat gcccgcggcc cggcccgcaa cggcccggcg
     3061 ctggtcctgc tcgacctcaa cctgcccgac atgagcggga tcgacattct cgcccggctc
     3121 aagggcacag aagaactgcg gcgcacgccg gtggttgtcc tgaccacgac cgacgacaag
     3181 gtcgagatcc agcgctgcta tgacctgggc tgcaacgtct acatcaccaa gccggtcaac
     3241 tacgag
//
LOCUS       DATNBV010000568         4983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1900723,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000568 DATNBV010000000
VERSION     DATNBV010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900723"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VN627_13465"
     CDS             <1..499
                     /locus_tag="VN627_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXD04305.1"
                     /translation="GKGIMLDTDVAPSLAARSFTITADLTGGDGVIAATGSSQGGWSF
                     AVENGHPVIHQAFSVMPSDQTHVVSSEALKPGQTARVTFDFDYDGGGAGKGGVLSIAI
                     DGRKVAEQRIARTILNGAPHAESFDIGLDSGVPVIWTDRGVNAYTGEIRKVTFDLGPQ
                     GRKRQ"
     gene            complement(502..657)
                     /locus_tag="VN627_13470"
     CDS             complement(502..657)
                     /locus_tag="VN627_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04306.1"
                     /translation="MAARRARAPAGRENVLIGSTTGQGNQQHGGATAAWALLAGYGVP
                     LAEGGGT"
     gene            719..1462
                     /locus_tag="VN627_13475"
     CDS             719..1462
                     /locus_tag="VN627_13475"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HXD04307.1"
                     /translation="MWWNHWRRGAGWLAAVVAGCGIGTLALGGYLGGSARTFTPVVPG
                     PAPAARPGMAAVFWSGDMGTWVGFGSDLPERLASKGIPVLTINTPALFGRARDAAFAR
                     AEVAGSLADAIRRSGARQVAVIGFSFGADVLAATLGQLDPALRQRIGPVVLVGPATGI
                     YFHANPFGLFYRGPSVADPRAAAASLRGLRLSCIFAAAEADESLCREPALRRSRLTQI
                     DDGHMMLFHREDVTRAVIASVLNPPEPLP"
     gene            1459..1893
                     /locus_tag="VN627_13480"
     CDS             1459..1893
                     /locus_tag="VN627_13480"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HXD04308.1"
                     /translation="MNPLLRWCGWLAAVLFSCSAALAAFAAFAAFAAPADLRGLWRNP
                     DGSVVVRIDSCGAEICGTVVRADATAQADARDGGYPRLIGLRLLYGYRPTSARHWVGR
                     VLVPDLGHVFSSHIDLIDQNHARIAGCLFHQHLCQTQVWERT"
     gene            1897..4428
                     /gene="mprF"
                     /locus_tag="VN627_13485"
     CDS             1897..4428
                     /gene="mprF"
                     /locus_tag="VN627_13485"
                     /inference="COORDINATES: protein motif:HMM:NF033480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HXD04309.1"
                     /translation="MLAAPRRLPIARQLAIVVATIAGTAIIGAALLHFARGLDPAQIH
                     EALAALSAGRITAALALTTESYVLLALFDGLALASLGEKVAWPVIGRASFTGYTVAHN
                     LGFGLLTGGSARLVIYRQAGVPPTKVARVVLMASAAFWLGIAAIAGAALLLGGGHLAL
                     FGVALSPLGARLAGAAVVALLVALPFSGRLLHRTSLRDLPPARFVAAMLGTTCADIAV
                     SVATLLVLVPSLGPGDFPQLFLAYALAIVAGLVTHVPGGLGVFEGTVLAALGRHGPEI
                     AAGLIAYRAIYYLLPLGVSLLLNGAIALRATYRRGGTISRALGFIAVEAGPVSMAAMV
                     FAGGLVLLLSGALPAVHDRLWALRQMVALPVVEAAHLAASLTGAALLLVAPALLSRSR
                     SGFVTARNLLLAGAVFSLAKGLDFEEAGLMLAMAGGLNGFAAAFYRQSMGAFSSHNRI
                     WLVAAMVAVVFTAVSGLSAYPNHSLLNGWWLRFAWRADRARYLRASFATCAMVAAFAV
                     RELLSRPPVKVGLAGLPAEAFIRATAHSGRSDAMLALTGDKRFLVSGRGDAFLMFRPL
                     GRTWFVMGDPVGQASLWGDLAWELRRQSDRVGARLCFYQISDAFLPLALELGLRPVKY
                     GEEAVVALGGFTLAGSRMKPLRNSHAQARRSGLVLDMVPAAQAAAHIDELRAISDAWL
                     ARHGGREKRFSLGAFDPDYLTRCDIAVVRGAGGSAVAFANLWRSGDGAELSIDLMRQR
                     PDAPAGTMDFLLVELIQQAARQGFARFNLGVAPLSGMRGGRLATAWTRGARLAFGFRQ
                     LRYNFKGLRRYKEKFAPEWHNRYIALPPGLAGYRALIQLLRLIGR"
     gene            4593..>4983
                     /locus_tag="VN627_13490"
     CDS             4593..>4983
                     /locus_tag="VN627_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HXD04310.1"
                     /translation="MVRPIFFDPTGRRRRRVRLSFLGALLMLVLASVLFATTVLEVPI
                     AAPLPIGYERMAPLPFRAQVSRLKHRVTGLFRPQPVTAAASGHPLTIAFYSPAMEDGT
                     ASLTHHVDQVDWLAPTTLSLDPGGTMTV"
BASE COUNT          598 a         1850 c         1666 g          869 t
ORIGIN      
        1 gggcaagggg atcatgctcg ataccgacgt cgccccctcg ctggccgcgc gctccttcac
       61 catcaccgcc gatctcaccg gcggcgacgg ggtgatcgcg gccaccggca gcagccaggg
      121 tggatggagc tttgccgtgg agaacggcca ccccgtcatc caccaggcct tctcggtcat
      181 gccgagcgac cagacccatg tcgtatcgtc cgaggcgctg aagcccggcc agacggcccg
      241 cgtcaccttc gacttcgact acgatggcgg cggcgccggc aagggcggcg tgctgtccat
      301 cgccattgat ggccgcaagg ttgccgaaca gcggatcgcc cgcacgatcc tcaacggcgc
      361 gccccatgcc gagagcttcg atatcggtct agacagcggc gtgccggtga tctggaccga
      421 tcgcggggtc aatgcctaca ccggggagat ccgcaaggtg accttcgacc tcgggccgca
      481 gggccgcaag cgccagtagg ctcaggtgcc gccgccctcg gccagcggca cgccgtagcc
      541 ggccagcagc gcccaagctg ccgtggctcc gccatgctgt tggtttcctt ggccggttgt
      601 gctgccgatc aggacgtttt cccgcccggc cggtgcccgt gcacggcgag cggccatgat
      661 gacggggatt taatctcccc cccatcggct gcttatcacg ccgcgctagt gaggcgtgat
      721 gtggtggaat cattggcggc gcggggcagg ctggcttgcc gctgtcgtcg cgggctgtgg
      781 catcggaaca ctggcgttgg gcggctacct gggtggcagc gcacgcacgt tcacgcctgt
      841 cgtacccggc cccgcgcctg ccgcgcggcc gggcatggcc gccgtgttct ggtcgggcga
      901 catgggcacc tgggtcggct tcggctccga cctgcccgag cgcctcgcaa gcaagggcat
      961 cccggtcctc accatcaata ccccggcgct gtttgggcgg gcacgcgacg cggcctttgc
     1021 ccgggcggag gtggccggga gcctcgccga tgcgatccgc cggtctggcg cgcggcaggt
     1081 cgccgtgatc ggcttctcct tcggcgcgga cgtccttgcc gcgacgctgg gccagctcga
     1141 tcccgccctg cggcaacgga tcggtccggt cgtgctggtc ggcccggcaa ccggcatcta
     1201 cttccatgcc aatcccttcg gcctgttcta ccgcggcccc tccgtagctg atccgcgcgc
     1261 ggcggcagcc tcgctgcggg gcctgaggct gtcctgcatc ttcgccgccg ccgaggccga
     1321 cgagagcctg tgccgcgagc ccgcgcttcg ccgtagccgc ctgacccaga tcgacgacgg
     1381 ccacatgatg ctgttccacc gcgaggacgt gacccgcgcc gtcatcgcct cggtgctcaa
     1441 tccgccggag cccctgccat gaacccgctg ctgcgctggt gcggctggct ggccgccgtc
     1501 ctcttctcct gctccgccgc cctcgccgcg ttcgccgcgt tcgccgcgtt cgccgcaccg
     1561 gccgacctgc gcggcctgtg gcgcaacccc gatggttcgg tcgtcgtgcg gatcgattcc
     1621 tgcggggccg aaatctgcgg cacggtcgtc cgcgccgatg ccacggccca ggccgatgca
     1681 cgcgacggcg gctatccgcg cctgatcggc ctgcggctgc tctacggcta ccgcccgacc
     1741 agcgcccgcc actgggtcgg ccgggtgctg gtccccgatc tcggtcacgt gttctcgtcc
     1801 cacatcgacc taatcgacca gaaccacgcg cggatcgccg gctgcctgtt tcaccagcac
     1861 ctgtgccaga cccaggtctg ggagcgcacc tgagccatgc tggccgcccc ccgccgcctg
     1921 ccgatcgcgc gccagctggc catcgtcgtg gccacgatcg ccggaaccgc gatcatcggg
     1981 gcggcgctgc tgcacttcgc ccgggggctc gatccggcgc agatccacga agccctggct
     2041 gcgctgtccg ccggccggat cacggccgcg ctcgccctga ccacggagag ctatgtgctg
     2101 ctggccctgt tcgatggcct tgccctcgcc agccttggcg agaaggtggc ctggccggtg
     2161 atcggccgcg cctcgttcac cggctacacg gttgcgcata acctcgggtt cggcctgctc
     2221 accggcggtt cggcccggct ggtgatctac cggcaggccg gtgtaccgcc gacgaaagtg
     2281 gcgcgggtgg tcctcatggc ctcggccgcc ttctggctgg gcattgccgc catcgcggga
     2341 gcggccctgc tgctgggcgg cggacacctc gccctgttcg gggtcgcgct ctcccccctg
     2401 ggcgcccgac tggcgggggc ggcagtcgtg gcgctgctgg tggccctgcc gttcagcgga
     2461 cgcctgctgc accgcacgag cctgcgcgat ctgccgcctg cccggtttgt agcggcgatg
     2521 ctgggcacca cctgcgccga tatcgccgtg tcggtcgcga ccttgctggt gctggtgcca
     2581 tcgcttgggc ccggcgattt cccgcagttg ttcctggcct atgccctcgc catcgtggcc
     2641 gggctggtga cccacgtgcc cggcggcctc ggcgtattcg agggcacggt cctcgccgcc
     2701 ctcggccggc acgggccgga aatcgctgcc gggctcatcg cctaccgggc catctactac
     2761 ctcctcccgc tcggcgtctc gctgctgctc aacggcgcta tcgccctgcg cgccacctac
     2821 cggcgcggcg ggaccatcag ccgcgccctc ggcttcatcg ccgtcgaggc ggggcccgtc
     2881 tcgatggcgg ccatggtctt tgccgggggc cttgtcctgc tgctgtcggg cgccctccct
     2941 gcggttcatg accggctctg ggccctgcgc cagatggtgg ccctgccggt ggtggaggcc
     3001 gcccacctcg ccgcaagcct caccggcgct gccctcctgc tcgtcgcccc cgccctgctt
     3061 tcacgcagcc ggtctggctt tgtgaccgcg cgcaacctgc tgctggcagg cgccgtgttc
     3121 tcgctcgcca aggggctgga tttcgaggaa gccgggctga tgctggccat ggccggcggg
     3181 ctcaacgggt ttgcggcagc cttctaccgc cagtcgatgg gcgccttttc cagccacaac
     3241 cggatctggc tggtcgcggc catggtggca gtcgtgttca cagcagtgag cggactttcg
     3301 gcctatccca accactcgct gctcaatggc tggtggctcc ggttcgcctg gcgcgccgac
     3361 cgcgcgcgct acctgcgcgc cagctttgcg acctgcgcca tggtggcggc cttcgcggtg
     3421 cgcgagctgc tctcgcgccc gccagtcaag gtcggcctcg ccggattgcc tgctgaagcc
     3481 ttcatccggg caaccgccca ttccggccgc agcgatgcga tgctggcgct cactggtgac
     3541 aaacgcttcc tcgtctccgg gcgcggcgat gctttcctga tgttccgccc gctggggcgg
     3601 acatggttcg tcatgggcga tccggtcggc caggcctcgc tctggggcga ccttgcctgg
     3661 gagctgcgcc ggcagagcga tcgggtgggc gcccggctgt gcttctacca gatcagcgat
     3721 gccttcctgc ccctggcgct cgagctgggc ctgcggccgg tgaaatacgg cgaggaagcg
     3781 gtggttgcgc ttggtggttt cactctcgcc gggtcgcgga tgaagccctt gcgcaacagc
     3841 catgcccagg cccggcgcag cggcttggtg ctggacatgg tgcccgcagc gcaagcggct
     3901 gcgcacatcg acgagctgcg cgccatatcc gatgcctggc tcgcccggca cggcggccgc
     3961 gagaaacgct tcagcctcgg cgccttcgat cccgactacc tcacccggtg cgacatcgcc
     4021 gtggttcgcg gcgccggcgg cagcgccgtg gccttcgcca acctgtggcg ctcgggcgac
     4081 ggggccgaac tgtcgatcga cctcatgcgc cagcgccccg acgcgccggc cgggaccatg
     4141 gatttcctgc tggtcgagct gatccagcag gcggcccggc aaggctttgc ccggttcaac
     4201 ctgggcgtcg cccccctgtc cggcatgcgc ggcgggcggc tggcgacggc ctggacccgc
     4261 ggcgcgcgcc tcgccttcgg gttccggcag ctgcgctaca atttcaaggg cctgcgccgc
     4321 tacaaggaga aattcgcgcc cgaatggcac aaccgctaca tcgccctgcc cccgggcctc
     4381 gcgggctacc gggcgctgat ccagctgctg cggctcatcg ggcgctaggg cgcgctgcat
     4441 gacgaaagcg taatacagcg gccactgcac ctgttataat catggcgtaa ttgaggccga
     4501 atggaaccgg ggcttccgac cgctcagcct gatcgctgga cgcgggcccc gcgtgccagc
     4561 cgtccctgtc gcagtcgaaa gtaggcaagt tcgtggtccg cccgatattc ttcgatccca
     4621 ccggtcgccg tcggcgccgg gtccggctct cgttcctcgg agccctgctg atgctggtgc
     4681 tggccagcgt gctgttcgcc acgaccgtgc tggaagtgcc catcgccgcc ccgctgccga
     4741 tcggctacga gcgcatggcg ccgctgccat tccgcgccca ggtgagccgc ctgaagcacc
     4801 gcgttaccgg cctgttccgc ccgcagccgg tcaccgccgc cgccagcggc catccgctga
     4861 cgatcgcctt ctattccccg gccatggaag atggcacggc ctcgctcacc caccatgtcg
     4921 accaggtgga ctggctcgcg ccgaccacgc tgtctctcga tccgggcggc acgatgactg
     4981 tct
//
LOCUS       DATNBV010000569         2396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_631798,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000569 DATNBV010000000
VERSION     DATNBV010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631798"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(441..1259)
                     /locus_tag="VN627_13495"
     CDS             complement(441..1259)
                     /locus_tag="VN627_13495"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXD04311.1"
                     /translation="MYLQVNGHPTYCYTGGKTFDATKPTVVFIHGVVCDHSVWILQTR
                     YLAHHGWNVLAVDLPGHCRSEGDAPSSVEEAADFIAGLLEAAGVSKAALVGHSWGSLI
                     ALEAAARLKERVSHLILVGTAYPMKVSAALLDASLSQPMKALEMINVFSRSTLAAPPS
                     ALGPGTWVYGASMALGQRVLASNPRVNVFHRGFKACDSYANGDKAIATIACPVLFVLG
                     SEDQMTPPKAAQSLIGAARESGKSVKIVALPVGHHQMTETPDATLFAIRDFLKT"
     gene            complement(1268..>2396)
                     /locus_tag="VN627_13500"
     CDS             complement(1268..>2396)
                     /locus_tag="VN627_13500"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006004.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HXD04312.1"
                     /translation="VYSRISNPTNAVLEQRMAALEGGIGAISTASGQAALHLSIATIM
                     GAGSHIVASTALYGGSQNLLHYTLRRFGIATTFVKPGDIDGWRAAIRPQTRLLFGETV
                     GNPGLEVLDIPQVAAIAHEAGVPLLVDSTLTSPYLMRPLALGADLVYHSATKFLSGHG
                     TVIGGVVVDGGSFDWERSGKFPELTTPYDGFHNMVFSEESTVGAFLLRARREGLRDFG
                     ACMSPHTAWLILQGIETLPLRMERHMGNTRKVVEFLAAHPFVASVGHPMLQSHPSYEL
                     AQQLLPKGAGSVFSFDIKGNREQGKKFIEALKVFSHLANVGDCRSLVIHPASTTHFRM
                     SDEALAAGGITQGTIRLSIGLEDPADLIDDLKRALKAAEKA"
BASE COUNT          410 a          765 c          809 g          412 t
ORIGIN      
        1 atactgcaca gcacgctcgt gctgctgcgc ttcttcaagc gtgttgaagg tgccaaggtt
       61 gcggcgcttg ccggtcttgg ggtcgacctt gcgcgagtac aggcgatagc cgccgttgga
      121 aagtttgcgg atcatgtcgc cagcttaagt tcattgcgcg aggccttcct gtcggctcgc
      181 agcgagcgcc ggcgtaggca gatgcgcacg acaccggagc cggacgtcag ctgcggacga
      241 gcaattcaac ccagtacgaa aggcgcttgc aggaaccctc tgaaccgcgc cggccgccgc
      301 gcgtgggcga agcagtgagg cggtagcccg gaaggcggcg cagaaaacgc ccgatccggc
      361 ggcagcgccc gcaggtcgaa cccggcggcg atttcccgtg cgcgcccccg tcatcggcgg
      421 cgtgatcagg ggaacggaac tcatgtcttc aggaagtcgc ggatcgcgaa gagcgtggca
      481 tcgggggttt ccgtcatctg gtggtgaccg accggcagcg ccacgatctt cacggacttg
      541 ccgctttccc gggccgcgcc gatcagcgat tgcgcggcct tgggtggagt catctggtcc
      601 tcggagccca gcacgaacag caccgggcaa gcgatcgtgg cgatcgcctt gtcgccattg
      661 gcatagctgt cgcacgcctt gaagccgcga tggaacacgt tgacacgcgg gttgctcgcg
      721 agcacccgtt gccccagcgc catgctcgcc ccgtacaccc aggtgccggg gccgagagcc
      781 gacggcggcg ccgcgagcgt gctgcgcgaa aagacgttga tcatctcgag cgccttcatt
      841 ggctggctga gcgacgcgtc cagcagcgcc gccgacacct tcatcggata tgcggtgccc
      901 accaggatca ggtgcgacac cctttccttg agcctggccg cggcctcgag cgcgatcagc
      961 gagccccagc tgtggccgac cagcgcggct ttggagacgc ccgcggcttc gagcaggccg
     1021 gcgatgaaat ccgccgcttc ttcgacgctc gatggagcat cgccttcact gcggcaatgg
     1081 cccggcaggt ccacggcgag cacgttccat ccatgatgcg cgaggtagcg ggtctgcagg
     1141 atccatacac tgtgatcgca aaccacgccg tgaataaaga cgaccgtcgg cttggtcgca
     1201 tcgaacgtct tgccgccggt gtagcagtag gtggggtggc cgttgacttg caggtacatt
     1261 gcgcctccta tgccttctcg gccgccttca gcgcccgctt cagatcgtcg atcaggtcgg
     1321 ccgggtcttc cagcccgatc gacaggcgaa tcgtcccctg tgtgatgccg ccggccgcca
     1381 gcgcttcgtc gctcatccgg aaatgagtgg tgctggccgg atggatgacg agcgagcggc
     1441 agtcgcccac gttggccaga tggctgaaca ccttgagggc ctcgatgaac ttcttgcctt
     1501 gctcgcggtt gcccttgatg tcgaagctga agacggagcc ggcgcccttg ggcaacagct
     1561 gttgcgccag ctcatagctg gggtgcgact gaagcatcgg atggcccacg ctggccacga
     1621 acggatgggc tgccaggaat tccacgacct tgcgcgtgtt gcccatatgg cgctccatgc
     1681 gcagcggcag ggtctcgatg ccttgcagga tcagccaggc ggtgtgcggg ctcatgcaag
     1741 caccgaagtc gcgcaggcct tcgcgccggg cgcgcagcag gaaggcgccg actgtcgact
     1801 cctcgctgaa caccatgttg tggaagccgt cgtagggggt ggtgagctcg ggaaatttgc
     1861 ccgaacgctc ccagtcgaaa ctgccgccgt ccaccacgac gcccccgatc accgtgccat
     1921 ggccggaaag gaatttggtg gccgagtggt agaccaggtc agcacccagg gccagtggcc
     1981 gcatcaggta aggcgacgta agtgtggagt cgacgagcag gggcacgccc gcctcgtggg
     2041 cgatcgcggc cacctgcgga atatcgagga cttccaggcc cgggttgccg acggtttcgc
     2101 cgaacagcag gcgcgtctga ggccgaatcg cggcgcgcca gccatcgatg tcgcctggtt
     2161 tgacgaacgt ggtggcgatg ccgaagcgcc gcagggtgta gtgcagcagg ttctgcgaac
     2221 ccccatacaa ggccgtgctc gccacgatgt gcgaccccgc gcccatgatg gtggcgatgc
     2281 tcaggtgcag ggccgcctgg ccgctggcgg tggaaatggc gccgataccg ccttccagcg
     2341 ccgccatgcg ctgctccagg accgcgttgg tcgggttgct gatgcgcgaa tacaca
//
LOCUS       DATNBV010000570         2272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2232937,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000570 DATNBV010000000
VERSION     DATNBV010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232937"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VN627_13505"
     CDS             complement(<1..435)
                     /locus_tag="VN627_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HXD04313.1"
                     /translation="MPAECALIRTAAPPDAPFALIEKQRGALRIVALSREAAALGLTP
                     GLPLADARARVPGLAAVPFDPEADAVLLGRLVAHCRVYTPSVMADPPQGLALDIAGCT
                     HPFSDGEAGLQRDLLGALAGKGLTARAACAGTPDAARALARFG"
     gene            complement(392..1141)
                     /locus_tag="VN627_13510"
     CDS             complement(392..1141)
                     /locus_tag="VN627_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04314.1"
                     /translation="MDAPYTPERGEAFLRILGIPRVDDWLGGGLRGDGLHEFYAAETA
                     DLTSALSVALLLGALPVGTGRPGLLWLRRGSRSVPYGPGLADLGIDPAAITILSVADD
                     RAVLRAALDGVRAGAVGTVLLELAGRQPLLDLTATRRLVLAAGETGTLVLLVRSGVKP
                     SPSAAHSRWRVASAPSIPLEADAPGQPAFDLSLLHYRGGREGLHIILEWNRDTASFRE
                     REDRPAAAPLSGRASAVDAGGVRPDPHSRAA"
     gene            complement(1241..1915)
                     /locus_tag="VN627_13515"
     CDS             complement(1241..1915)
                     /locus_tag="VN627_13515"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXD04315.1"
                     /translation="MKLAIRTIRSAVIGLLLLGLIVFVPAGTLAYWQGWAFIIVFTIS
                     TNIMGVYLALKDPALLERRIKAGPGAETRPAQKVLISIAFAGMIALVVVSALDHRFGW
                     SHVPVWVSLLGNVLVALGLMIDLRVFRENSYGASTIRKMEGQKVISTGPYALVRHPMY
                     VGALVMVMGVPLALGSYWGLLFMLLNVPVLMLRILDEEKMLGSELEGYTAYARTVRYR
                     LIPGLW"
     gene            complement(1946..>2272)
                     /locus_tag="VN627_13520"
     CDS             complement(1946..>2272)
                     /locus_tag="VN627_13520"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HXD04316.1"
                     /translation="VTGALMLDRWIGAALGYGVLMAIALAYRALRGRDGLGFGDAKLL
                     GAIGAWAGWQALPLVLLFASGGGLLWALVLALVGRDVGHKTRLPLGTFLCLAVLPALL
                     SGAMLW"
BASE COUNT          454 a          802 c          726 g          290 t
ORIGIN      
        1 gccaaagcgg gccagcgcac gcgccgcatc gggtgtgccg gcacaggccg cacgggcggt
       61 caggcccttg cccgccaatg cgccgagcag gtcccgttgc aaccctgctt ccccgtcgga
      121 gaaggggtgc gtgcagccgg caatgtccag ggccagaccc tgcggcgggt cggccatcac
      181 gctgggagta tagacacgac aatgcgccac cagccggccg agcaggacgg catcggcctc
      241 cggatcgaac ggcacagcgg caagccccgg cacccgggcc cgcgcatccg ccagcggcag
      301 gcccggcgtc aggcccagcg cggcggcctc tcgcgacagc gcgacgatgc gcagggcacc
      361 ccgctgtttc tcgatcaggg cgaaaggcgc atcaggcggc gcggctgtgc ggatcagggc
      421 gcactccgcc ggcatccacg gcagatgcac ggccagatag cggcgcggcg gcgggccggt
      481 cctcccgctc gcggaaagag gctgtgtcac ggttccactc caggatgatg tgcaatcctt
      541 cccgcccgcc acgatagtgc agcaagctga ggtcgaaggc gggctggccg ggcgcatcgg
      601 cctccagcgg gatcgagggt gcgcttgcca cccgccagcg gctgtgcgcg gcgctggggg
      661 atggcttgac gccggaccgg accaacagca ccagcgtccc ggtctcgccc gccgccagca
      721 ccagccgccg ggtcgcggtg agatcgagca gcggctgccg cccggccagt tccagcagca
      781 ccgtgccgac tgccccggcg cgaacaccat cgagcgcggc gcgcaggacc gcgcggtcat
      841 cggcgacgct caggatcgtg atcgcggcgg ggtcgatgcc cagatcggca aggcccggcc
      901 cgtaaggcac gctccttgaa ccgcgccgca accagagcag gccaggccgc cccgtcccca
      961 ccggcaacgc gcccagcagc agcgcgacgc tcagcgcgct ggtgaggtcc gcggtttcag
     1021 cggcgtagaa ttcatggaga ccatcgccgc gtagcccacc gccgagccaa tcatcgacgc
     1081 gaggtatacc aagtatacgc aagaacgctt cgccgcgttc tggcgtgtag ggcgcatcca
     1141 acaggctccg aaaagccgcc gggcgcaggg ggagaaacga ctcacggcgc atatgttcac
     1201 tttatgttct attttgtgcg ccatcctgtc tcctgtcaac tcaccaaaga ccggggatga
     1261 ggcgatagcg taccgttcgc gcataggctg tgtagccctc cagctccgat ccgagcattt
     1321 tctcttcgtc caggatacgc agcatcagga ccggcacgtt gagcagcatg aacagcagac
     1381 cccaatagga gcccagcgcc agcggcacgc ccatgaccat gaccagtgcg ccgacataca
     1441 tgggatgccg cacgagagca taagggccgg tggagatgac cttctggcct tccatcttcc
     1501 ggatcgtcga cgcaccatag ctgttctcgc gaaagacccg gaggtcgatc atgagcccga
     1561 gcgccacgag gacattgccc aacagcgaga cccacacagg cacatgcgac cagccgaagc
     1621 gatggtcgag cgcggagacg accaccagcg cgatcatgcc ggcgaaagcg atggaaataa
     1681 gcaccttctg cgccggccgc gtttcggcac ccggcccagc cttgatacgc cgttccagca
     1741 gtgcaggatc cttgagcgcc agatagacac ccatgatgtt cgtcgagatc gtgaagacga
     1801 taatgaaggc ccagccctgc caataggcca gcgttccggc cgggacgaag acgatgaggc
     1861 caaggaggag caggccgatg acggcggacc tgatagtgcg aatggcaagc ttcatcgcaa
     1921 ttcccggctg aaattcaacg atattctacc agagcatcgc ccccgacaaa agcgccggca
     1981 ggacggcaag gcacaggaac gtgccgagcg gcaggcgcgt cttatggccg acatccctgc
     2041 cgaccagcgc gaggaccagc gcccagagca gcccgccgcc ggacgcgaac agcagcacca
     2101 gcggcagggc ctgccagccg gcccatgcgc cgatcgcacc cagcagcttg gcatcgccga
     2161 accccagccc atccctgccc cgcaacgccc ggtaagccag cgcaatcgcc atcaggacgc
     2221 catagcccag ggcggccccg atccagcgat ccagcatcaa cgcccccgtc ac
//
LOCUS       DATNBV010000571         2237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_633059,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000571 DATNBV010000000
VERSION     DATNBV010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633059"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1190)
                     /locus_tag="VN627_13525"
     CDS             complement(207..1190)
                     /locus_tag="VN627_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04317.1"
                     /translation="MYSHLRFVIAAALIAFGSVIAVGVPALLLAVDRPLLAQTDVSEP
                     PAASSCGQQGWLHFDRNCSQRGLPWAAARGTANAGPVEAPLEPAADQPLTEGRQAATA
                     PEPSAAQFSMRQLSEPQLFAPQISVPQSFTSRDTAAREVPPPESATNDAAPRTAAAKD
                     TAPQVTAPQVTAPQAAAPQVAAPQVAAPPVAAPQAKAPAVAAPVEAMRQELATRESAP
                     QEVGPGQTVEQSEVTAPVRTRSVRRAPAEERHVAQSPATAQTRAPLSKRVARGERTVK
                     RPASEALNAVRKFDDALPEIPANSYAADGTPRRIVIRPTSIQDVYYYSAPR"
     gene            1499..>2237
                     /locus_tag="VN627_13530"
     CDS             1499..>2237
                     /locus_tag="VN627_13530"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXD04318.1"
                     /translation="MPAATIATIDTIPLRLPLDTWAPPPQFAGKPRTHVDMLLVRVTT
                     SGGVVGWGEAYGSSSPMIPVVFDSWIKHVAIGQDATDRGLTARLERLLHGFGRCGPVV
                     HAISGLDIALWDIRGKLEGKPVSALLGGVRRKRVEAYASLLQYGGSIEHVRRNVARAL
                     ERGYRHLKLHERTADSVAAAREVAGPDIPIMVDTNCAWTADQATAPVAAMAPSKPFWV
                     EEPIWPPEDFDALAVLRRATGVPLAMGE"
BASE COUNT          350 a          737 c          790 g          360 t
ORIGIN      
        1 acgccgccgg ggccggtgac cggacagacc tcgacgcact tgccgcagcg ggtgcaagcg
       61 tccgccatcg cctcgacgcg tgcggccagc gcggtttcga aggtgacggt catgcgggca
      121 cgacctttcg ttcgcccagt cgcggtgaga ttaccgattc gaccccggcg atgaaacccc
      181 actcccgcgg cttggatgaa ctcgcgctat cgcggtgcag agtagtaata gacgtcctgg
      241 atcgacgtgg gccggatgac gatcctgcgc ggtgtaccgt cggcggcata agagttcgca
      301 ggaatctcgg gcagggcatc gtcgaatttc cgcacggcat tcaaggcttc gctcgccggt
      361 cgtttcacgg ttcgctcccc gcgagcgact ctcttcgaga gcggcgcacg ggtctgcgca
      421 gtggccgggg attgcgcgac gtgccgttcc tcggcgggcg ctcgccgtac cgacctcgtc
      481 cgcacaggcg ctgtcacctc gctctgctcc accgtctgcc cgggtccgac ctcttggggt
      541 gcggattcgc gggtcgccaa ttcttggcgc atggcttcca cgggcgcggc caccgctggt
      601 gccttcgctt gtggggccgc tactggtggc gccgctactt gcggtgccgc tacttggggc
      661 gccgccgctt gtggcgccgt tacttggggc gctgtcactt ggggcgccgt gtctttggcc
      721 gccgccgttc ggggcgcggc gtcattggtt gcggattccg ggggagggac ctcccgggcc
      781 gccgtgtctc gggatgtgaa actctgcggc acggagatct gcggcgcgaa cagttggggc
      841 tcggaaagct ggcgcatgga gaactgcgcc gccgacggct caggtgcggt cgctgcctgc
      901 cggccttccg tcaaaggctg gtcggctgcc ggttcaagcg gggcctcgac cgggccggcg
      961 ttcgcggtcc cgcgtgccgc ggcccagggc aggccgcgct gggaacagtt ccgatcgaag
     1021 tgcagccagc cctgctgccc gcaagaggag gcggcaggcg gctcggaaac gtcggtttgg
     1081 gcgagcagtg gccgatcaac ggcaagcagc aaggccggca cgccaacggc gatcacgctt
     1141 ccgaaggcga tcagggcggc ggcgatgacg aaacgcagat gcgagtacaa ggcccatctc
     1201 cttcaggccg cccccgcgcc gcgccgattc cgggcaagaa caacggtggc ggcgggacta
     1261 tgtccgtttg gggggccggc tcccagcgga aatcgaaact gtgtttaacg cgtcgcggcc
     1321 cttgggacca gggcgccacc agacggaaac tcgggcccaa agggaaaacc ctacagtctg
     1381 gccagagttc cgacagcggc cgacccgccg gcgtttgcca aacaccaaga agtacgctcg
     1441 attggtcgca acagcggcgt aaggccgttg acatataacg acgcaccaag gacccgcaat
     1501 gcctgccgcc accatcgcca ccatcgacac aataccgctt cgcctcccgc tggacacctg
     1561 ggcgccgccg ccgcaattcg cgggcaagcc gcggacgcac gtggacatgc tgctggtgcg
     1621 cgtcaccacc agcggcggcg tggttggctg gggcgaagct tacggcagca gttcgcccat
     1681 gatcccggtc gtgttcgatt cctggatcaa gcatgtcgcc atcggacagg atgcgaccga
     1741 ccgcggcctg actgcgcggc tggaacgcct gctgcatggc ttcggtcgct gtggcccggt
     1801 ggtgcatgcg atcagcgggc tcgacatcgc gctgtgggac atccgcggca agctcgaagg
     1861 caagccggtg tcggcgctgc ttggcggggt gcggcgaaag cgcgtggaag cctatgcgtc
     1921 gctgctgcaa tacggcgggt cgatcgagca cgtgcgccgc aacgtcgcgc gggcgctcga
     1981 acggggctac cgtcacctca agctgcacga gcggaccgcg gacagcgtcg cggcggcacg
     2041 cgaggtggcc gggcctgaca tcccgatcat ggtcgatacc aactgcgcct ggactgcgga
     2101 ccaggccacc gcgccggtag ccgccatggc gccctcaaag ccattctggg tcgaggagcc
     2161 gatctggccg ccggaggact tcgacgcgct ggcggtgctc aggcgcgcaa ccggggtacc
     2221 gctggcgatg ggcgaga
//
LOCUS       DATNBV010000572         3791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3826890,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000572 DATNBV010000000
VERSION     DATNBV010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3826890"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2156)
                     /locus_tag="VN627_13535"
     CDS             complement(<1..2156)
                     /locus_tag="VN627_13535"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HXD04319.1"
                     /translation="MALGASAERRPVLDQIAVPHDYYYREMYLPQLSSGPSGVAFTPD
                     GRSLVYSMQGSLWIQPLDSGTATQLTAGPGYDYQPDVAPDGKRVVFVRYLADALELNV
                     LDLESGTVQQITSGGAVNCEPRWSPDGKRIAWVSTNGTGHFHVFVGDLNDGKLVGGAA
                     WPERKSHTARYYYSAYDHELSPSWSPDGRELIYVGNPEIIYGTGSIWRRALTSGAEPR
                     LIRQEETTWRARPDWSPDGKRIVYASYTGRNGHQIWSTTSAGNGDPLALTYGAGEATG
                     ARWSADGRRVAYLSNRSGDNQIQVMDIPGAKTRTIAVRERHYLKPMGRLQLHVTGVAA
                     HGPPVSARIAVVAADGRAYAPDDAWMHAEDGYDRKLAAFETHYFHSQGNSTLSLPPGL
                     AKITVYRGLATQIARSEILVRAGETVAVNVALSPLALPPAWQTSWHSADVHVHMNYAG
                     AYRNTPEHLVRQADAEDLDVVFDLVVNKEQRIPDYDYFSPLPDAASTPAVLISHGQEF
                     HTSYWGHLGLLGLNDHFLLPGYAAYANTAAASLYPTNAAVADLAHAQSALVGYVHPYD
                     HVPDPAHDATLTSELPVDVALGKVDYYEVVGFSDNHRATAEVWHRLLNCGFRVAAAGG
                     TDAMANFASLHGPVGLYRVYVQDGAHATSNYGAPELKSRLANWLQALKAGHSLATNSA
                     LLGLEVEGKGPGSEVALTPGHSSVHVKGFMRSIVPI"
     gene            complement(2203..3540)
                     /locus_tag="VN627_13540"
     CDS             complement(2203..3540)
                     /locus_tag="VN627_13540"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HXD04320.1"
                     /translation="MNSVPKPKITRPTLPGPKARALIERDRAVVSPSYVRDYPFVMDR
                     GRGAEVWDVDGNRFIDFASGIAVCSTGHSHPDVVAAIKSASERFLHISSDYWHQGQVE
                     LGEAIAALDPVSGPALSFFANSGTEAVEAALKLARQVSGRSRFIGFLGGFHGRSMGSL
                     AFTASKYTQQRGYFPTMPGVTHVPYPNAYRPLFAGDDQGAAVVAYIEQLMERNVPASE
                     VAAILIEPIQGEGGYVVPPAGFLAKLRALCDKHGIYLIFDEVQTGIGRTGKMFAWQHT
                     GVRPDIMALAKGLGSGLPIGLMVAARKIIEKWPPGSHANTYGGNPICCAAALATLKLV
                     SGSYTENARAMGERAMQRLKQFAQEFSIIGDIRGAGLMIGMEFVKDRATKVPAKEFAT
                     RLVARAYENGLLLLECGASGIRIIPPLMIEPKVLDEGLDMLHQSFQEVLAEQD"
     gene            complement(3537..>3791)
                     /locus_tag="VN627_13545"
     CDS             complement(3537..>3791)
                     /locus_tag="VN627_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXD04321.1"
                     /translation="VNDSELGLTAGFFGAPDEVDWFFDKVEAGVAYSNRSQGATTGAW
                     PGFQPFGGWKGSGTTGKGIASSYYLALYQREQSQTRVESL"
BASE COUNT          655 a         1232 c         1221 g          683 t
ORIGIN      
        1 tcgatcggca cgatcgatcg catgaacccc ttcacgtgca cgctgctgtg cccgggcgtc
       61 agtgcaactt cgctgcccgg ccctttgcct tcgacttcga gcccgagcaa cgcgctattg
      121 gtcgccaggc tgtgcccggc tttcagcgcc tgcagccagt tggccaggcg gctcttcagt
      181 tcgggcgctc cgtagttgct ggtggcatgc gcgccgtcct ggacatacac acggtagagc
      241 ccgaccgggc catggagcga agcgaaattg gccatcgcgt ccgtcccacc cgccgcggcg
      301 acgcgaaatc cgcagttgag caaccgatgc cagacttcgg ctgtggcgcg gtgattgtcg
      361 gagaagccga ccacctcata gtagtcgacc ttgcccagcg ccacatcgac aggcaactcg
      421 ctcgtcagag tcgcatcgtg ggccggatcc ggcacgtgat cgtaagggtg cacatacccg
      481 accagcgccg attgggcgtg tgccagatcg gccaccgccg cattggtggg gtacaggctg
      541 gccgctgcgg tgttcgcgta ggccgcatag ccgggcagca ggaagtggtc attgagcccc
      601 agcagcccca ggtgccccca gtagctggta tgaaattcct gaccgtgtga gatcagcacc
      661 gcgggagtcg aggccgcatc gggtagcggt gagaaataat catagtcggg aatgcgctgt
      721 tccttattca cgaccagatc gaacaccacg tccaggtcct cggcatcggc ttgccgcacc
      781 aggtgctcgg gcgtgttgcg gtaggcaccg gcgtaattca tgtgcacgtg gacatcggca
      841 ctgtgccagc tggtctgcca agccggcggt agtgccagcg gactcagcgc cacgttgaca
      901 gcgacggttt ccccggcgcg caccagaatt tcgctgcgcg cgatctgggt ggccagtccg
      961 cgatacaccg tgatcttcgc cagccccggg gggagcgaga gcgtggagtt accctggctg
     1021 tgaaagtaat gagtctcgaa cgctgctagc ttccgatcgt agccatcctc ggcgtgcatc
     1081 caggcatcgt cgggcgcgta tgcgcggccg tcggcggcca ctaccgcgat gcgcgctgag
     1141 accggcggac catgggccgc aactccggtg acgtgcagct gcaagcgccc catgggcttg
     1201 aggtagtgcc gctcgcgaac cgcgatagtg cgcgtcttgg cgccgggaat atccatcacc
     1261 tggatctggt tgtccccgct gcgattgctg agataagcca cgcgccgtcc gtcggccgac
     1321 cagcgcgcgc cggtagcctc gcccgccccg taggtcagcg ccagagggtc gccgttgccg
     1381 gccgatgtcg tgctccatat ctgatgtccg ttgcggccgg tgtatgaggc atacacgatg
     1441 cgcttaccgt ccggtgacca gtcgggccgc gcgcgccagg tggtttcttc ctgtcgtatc
     1501 aggcgcggtt cggccccgga ggttagcgcg cgccgccaga tcgagccggt gccgtaaatg
     1561 atttcggggt tgcccacgta gatgagctcg cgcccgtccg gggaccagct gggcgagagc
     1621 tcatgatcgt aggcactgta gtagtaacgg gcggtgtggc tcttgcgctc cggccatgcc
     1681 gccccgccga cgagcttgcc gtcgttaaga tcgcccacaa acacgtgaaa gtgaccggtg
     1741 ccgttcgtcg acacccaggc gatgcgcttg ccatccggcg accagcgcgg ctcgcagttc
     1801 acggccccgc cgctggtgat ctgctgcacc gtgccgctct ccaggtccag cacattgagc
     1861 tccagagcat cggcgaggta gcgaacaaac actacccgct tgccgtcggg agcgacgtct
     1921 ggctgatagt cgtaacccgg gccggcggtc agttgggtgg cagtgccgga atccagaggc
     1981 tggatccaca ggcttccctg catggagtac acgagcgagc gcccgtcggg cgtgaaggca
     2041 acgccgctcg gcccgctgct gagctgcggc aggtacatct cgcggtagta gtaatcgtgc
     2101 ggtacggcga tctgatcgag caccggccgg cgctcggccg aggccccgag cgccatgcac
     2161 agcagcgcgc tcgcgcccgc caaggcccgc agtcgccgca agctagtcct gctccgcgag
     2221 tacttcctgg aaggactggt gcagcatgtc cagtccttca tccagtacct taggctcgat
     2281 catgagcggc ggaatgattc tgattccgct ggcaccgcac tccagcaata acaagccgtt
     2341 ctcgtaggcc cgcgccacaa gccgggttgc aaattccttg gccgggacct tggtggcacg
     2401 gtccttaacg aattccatgc cgatcatcag cccggctccg cggatatcgc caatgatcga
     2461 gaattcctgc gcgaactgct taagccgctg catggcgcgc tcgcccatgg cgcgcgcgtt
     2521 ctcggtgtag ctgccgctga ccagcttgag cgtcgccagc gcggccgcac agcaaatggg
     2581 attgccgcca taggtattgg cgtgcgaacc gggcggccac ttctcaatga tcttgcgggc
     2641 ggcgaccatc aggccaatgg gcaatccgga gcccaggccc ttggccagtg ccatgatgtc
     2701 cgggcgcacg ccggtgtgct gccaggcgaa catcttgcca gtgcggccga tgccggtttg
     2761 cacttcatca aagatcaggt agatgccgtg cttgtcgcac agtgcccgaa gcttggccag
     2821 gaaccccgcc ggcggcacga cgtagccgcc ctcgccctga atcggctcga tcagtatggc
     2881 cgccacttcg ctggcaggga cgttgcgctc catcagctgc tctatatagg caacgaccgc
     2941 cgcgccctga tcgtcgcccg cgaacaacgg gcgataggca ttcggatacg ggacgtgggt
     3001 tacgccgggc atggtcggga agtaaccgcg ctgctgcgtg tacttcgagg ccgtgaaggc
     3061 cagcgagccc atcgagcgcc cgtggaatcc gcccagaaag cctatgaaac gcgagcgccc
     3121 gctgacctgg cgtgcaagct tgagcgcggc ttccaccgcc tcggtgcccg aattggcaaa
     3181 gaagctgagc gccggaccgc tgaccgggtc cagcgcggca atcgcctcgc caagttccac
     3241 ctggccctgg tgccagtaat ccgaagagat gtgcaggaag cgctcggagg cggacttgat
     3301 cgcggcgacc acgtccggat ggctgtgtcc ggtcgagcac acggcgatgc ccgatgcaaa
     3361 atcgatgaag cgattgccgt ccacatccca gacttccgcg ccacgacccc ggtccatgac
     3421 gaatggatag tcacgcacgt aactggggga aaccaccgcg cggtcgcgtt cgatgagcgc
     3481 gcgggccttc ggccccggca acgtcggtcg cgtgatcttc ggcttgggta cgctgttcat
     3541 agggattcca cccgtgtttg tgattgttcg cgctggtaga gcgccaggta atagctagat
     3601 gctatgccct tgcctgtggt tccggaaccc ttccatcccc cgaagggctg aaagccgggc
     3661 caggcgccgg tcgtagcgcc ctgcgaacgg ttgctgtagg cgacgcccgc ctcgaccttg
     3721 tcgaaaaacc agtccacttc gtccggcgcg ccgaagaatc cggcagtcag gccgagttcg
     3781 ctgtcgttga c
//
LOCUS       DATNBV010000573         7014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2531216,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000573 DATNBV010000000
VERSION     DATNBV010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531216"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="VN627_13550"
     CDS             <1..156
                     /locus_tag="VN627_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HXD04322.1"
                     /translation="AYMRRCGFDSFAPEHPLNQADAEAAMARYPDVYTPTVDGRAPIW
                     AKRHHSA"
     gene            182..991
                     /locus_tag="VN627_13555"
     CDS             182..991
                     /locus_tag="VN627_13555"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HXD04323.1"
                     /translation="MTEHPLSAERKLDRIDTGPRFTEADAVRLNRMFRGAETVEWLSS
                     VLKDQLAGDVAVVSSFGAESAVLLHLVSTIAPETPVLFLDTGKHFPETLAYRDTLVAR
                     LGLNLVVLTPDAGELAKRDESGLRWSYDPDGCCELRKVKPLARALEKFDASITGRKAF
                     QSATRANLPRFEIDKSDAQGRLKINPLIDWTAEDLAAYIAAHDLPPHPLVAQGYPSIG
                     CSPCTHKVEAGEDPRSGRWKGWDKTECGIHVPGEDEGKPGDELPPGYDPVF"
     gene            1095..3989
                     /locus_tag="VN627_13560"
     CDS             1095..3989
                     /locus_tag="VN627_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HXD04324.1"
                     /translation="MLSRLIPTRLVLHASLLLSLVLAACAPQVAPPASTQAQPLRPRP
                     VWAMEESDIPLDPAFRASRLANGMRFIVRPNGTPKGTMLVRMEVEAGSLDETEAERGY
                     AHFVEHMAFNGSTHVPEGEMVRLLERKGLAFGADTNAQTNFHYTLYTLDLPRADPALL
                     DTALMLMRETASELTISPEAVARERGVVLSEMRDRNTWGFRAAVDEMRFTNLGARYAE
                     RLPIGTPEALNAATADSLRAFWQRTYVPAKTTIVVIGDLPADTAEAAIRKHFEDWQAA
                     PSPSQPSAGPVRFADKGRTAVYLDPALSERVTASRNGPYEDLPDSVAKRRENLLRQIG
                     YDIIGRRFQRIARQPPAPGQSPPFRAATFSTGDVFRAGRTTSLEVHTVDGGWRKGLLA
                     AAREYRRALSYGFTRDEVAEQVAITRTAHRNAVGASTTRANTALVNAALALVRDDTVP
                     ATPESSLARLEAFIPAITPEAVLAALKREALPLDQPLLRFAGRRAPDGGAPAIRAAWD
                     EAMRGKITRSGTSAPAEFAYTDFGPAGRVLSDTREPALGIREIRFANGVRLNLKQTGL
                     EQDRVLVQLSLDGGDMLVTHDDPLATEMMSAFAAGGLGQHSQDQLQSILAGRTVGVDL
                     QSGPETFTAISQTTPADLELQLQLMAAYVVDPGYRPEGETIYRQNVNNFFAQRFATPG
                     SALGSSLGGILSDNDPRFTFQKVEDYRALTFAKLKGEISDRLRRGAIEIGIVGDIPED
                     EAIALVARTFGALPPREAEFLPYAEQHERPFTRDLSRRIVRHTGPADQSIVRFTWPTR
                     DGEDPVAAMKLELLEKVMRIELIETIREKLGKAYSPAADSETSRTWRGYGTFAVAASV
                     NIADLAATRAAILETVAELRDRPVSADVIQRAREPMLEALDNALKTNRSWLSLVDRAQ
                     TEADRIDRQLKARERLSELTAADVQAIARQYLVPDHAVEVDVIPEGAPAP"
     gene            4154..6169
                     /locus_tag="VN627_13565"
     CDS             4154..6169
                     /locus_tag="VN627_13565"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HXD04325.1"
                     /translation="MGRNTAGNVLALVAAALPPILILVGGAVDMGRAYMAQTSLQNAC
                     DAGVLAGRRAQAKSGTWGTAEQAKAQKMFDINYQGLAANATSTSFVPTNPSVGVISAT
                     ATSTIPTTIMKIFGTNTFTLTASCSAEFQISNIDVMMVLDTTGSMAYCPDGSWCNSGA
                     GSKIEGLKSAIRSFYYTIAAAVPSGSGTRVRFGFVPYSDTVNVKNLTSGASPDIPQGY
                     IASAANYQTRLYNFDQASYTSTTGSPVVTQETYGGSATPIAISSADCDNYGINAYPTN
                     GGNPVTTGTAPDDVITKTYSKKSWTKTGTITLSGCGNCTGNNGQGNGNNGTKVDVGTC
                     VRQVSTVTTSYVLNGYKLNSTTPYRYTQASLNTSGLKSYAAVNLATEVYSNSTVPTAG
                     LYDMVTLGSLAGTTNILKVSTTWNGCIEERSTVNTLISAASIPSGATDLDVLTAPTSA
                     AATQWKPYISDFQFNRGQTATLDTTDDKDPMTAYCPAPAKNFTTVDTSAPTTVPSWIE
                     TYLGTLYASGGTYHDIGMIWGARLANPAGINSTVVNSGNVSSISRHIIFLTDGIMEPR
                     CDFLYSAYGVEYLDNRVMPAQPTRDAACDTTLATYHNGRYLAACQRAKDMGYTIWVIG
                     FGQAITPEMSACAAGSGGQAMTASSTTALQDTFRLIASRIANLRLRS"
     gene            6166..6789
                     /locus_tag="VN627_13570"
     CDS             6166..6789
                     /locus_tag="VN627_13570"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HXD04326.1"
                     /translation="MTHRSEHHRRPDGAHLRRLAADRSGAALVEFAIIIPVVALLLLG
                     SIEFGLNVYLRAVLEGAMQQAGRNSTLQSAQAGQTAIDTFVRDRVQAILPNATVAFTR
                     ENYYTFSTVGRPEDFTDTNGNGVHDANECFQDANGNNTWDADGARTGNGGANDVVEYT
                     ATVTYPSLIPGGTAIGLSPSTRISATTLLRNQPFANQPSWTSTQVCP"
BASE COUNT         1210 a         2767 c         1991 g         1046 t
ORIGIN      
        1 gcctacatgc gccgttgcgg gttcgacagc ttcgcccccg agcacccgct gaaccaggcc
       61 gacgccgagg cggcgatggc ccgctacccc gacgtctata cccccacggt cgacggccgc
      121 gcgccgatct gggccaagcg ccaccactcg gcctgaccgg ccgcaaccga ggctcattgc
      181 catgacggag caccccttga gcgccgaacg caagcttgac cggatcgata ccggacctcg
      241 cttcaccgag gccgatgcgg tgcgcctcaa ccggatgttc cgcggcgccg agacggtcga
      301 atggctgtcc tcggtgctca aggaccagct cgccggcgat gttgccgtgg tctccagctt
      361 cggcgcggaa agcgcggtgc tgctccatct ggtcagcacg atcgcgccgg agaccccggt
      421 gctgttcctc gacaccggca agcacttccc cgagaccctc gcctatcgcg acacgctcgt
      481 cgcccggctg ggcctcaacc tggtcgtgct caccccggat gccggggaac tggcgaagcg
      541 agatgaaagc gggcttcgct ggtcctacga tcccgatggc tgctgcgagc tgcgcaaggt
      601 caagccgctg gcccgcgcgc tggaaaagtt cgacgcctcg atcaccgggc gcaaggcctt
      661 ccagtccgcc acccgcgcca atctcccccg cttcgagatc gacaagtccg atgcccaggg
      721 gcgcctgaag atcaacccgc tgatcgactg gacggccgaa gacctcgccg cctacatcgc
      781 cgcgcacgac ctgccgcccc acccgctggt ggcgcagggc tatccctcga tcggctgctc
      841 gccctgcacg cacaaggtcg aggccggcga agacccgcgc tcgggccgct ggaagggctg
      901 ggacaagacc gaatgcggca tccacgtgcc cggcgaggac gaaggcaagc ccggggatga
      961 gctgccgccg ggctacgatc cggtattctg accgccagtc ccgtcgcccc cttctcccgg
     1021 ccccgatccg gccgctgcac ccggccgccc acaccccctt gcccgccgcg ccgcggcccc
     1081 taccctcacg ctccatgctc tcccgcctga ttcccacccg cctggttctt cacgcctccc
     1141 tcctcctctc gctcgtcctc gccgcctgcg cgccccaggt cgcgccgccc gcctccaccc
     1201 aggcgcagcc cctccgcccg cggcccgtct gggcgatgga ggagagcgac attccgctcg
     1261 atcccgcctt ccgcgccagc cgcctcgcca acggcatgcg cttcatcgtc cgccccaacg
     1321 gcacgcccaa gggcaccatg ctggtgcgca tggaagtcga ggccggctcg ctcgatgaaa
     1381 ccgaggccga gcgcggctat gcccatttcg tcgagcacat ggccttcaac ggctccactc
     1441 acgtgcccga gggcgagatg gtgcgcctgc ttgaacgcaa gggcctggcc ttcggggccg
     1501 acaccaacgc ccagaccaat ttccactaca cgctctacac gctcgacctg ccccgcgccg
     1561 atccggccct gctcgatacg gcgctgatgc tgatgcgcga gacggcgagc gagctgacca
     1621 tctcgcccga ggccgtggcg cgcgagcgcg gcgtggtcct gtccgaaatg cgcgaccgca
     1681 acacctgggg ctttcgcgcc gcggttgacg agatgcgctt caccaacctc ggggcgcgct
     1741 acgccgagcg cctgccgatc ggtacgcccg aggcgctgaa cgccgccacc gccgattccc
     1801 tgcgcgcctt ctggcagcgc acctacgtgc cggcgaaaac caccatcgtc gtcatcggcg
     1861 atcttcccgc cgacacggcc gaggccgcca tccgcaagca cttcgaggac tggcaggccg
     1921 ctccctcccc gtcccagccc tccgccggtc ccgtgcgctt cgccgataag ggccgcaccg
     1981 cggtctacct cgatcccgcc ctgtccgagc gtgtcaccgc ctcgcgcaac ggcccctacg
     2041 aggacctgcc cgacagcgtc gccaaacgcc gcgaaaacct gctgcgccag atcggctacg
     2101 atatcatcgg ccgccgcttc cagcgcatcg cgcgccagcc gcccgcgccc ggccaaagcc
     2161 ccccgttccg cgccgccacc ttctccactg gcgacgtgtt ccgcgccggg cgcacgacca
     2221 gcctcgaggt tcacacggtc gacggcggct ggcgcaaggg cctcctcgcc gcggcccgcg
     2281 aataccgccg cgccctgtcc tatggcttca cccgcgacga ggtggccgaa caggtcgcca
     2341 tcacccgcac cgcccatcgc aatgcggtcg gcgcctcgac cacccgcgcc aatacggcgc
     2401 ttgtcaatgc cgccctcgcc ctggtgcgcg acgataccgt gcccgcgacg cccgaaagct
     2461 cgcttgcccg gctcgaagcc ttcatccccg caatcacccc cgaagcggtc ctcgccgcgc
     2521 tcaagcgcga ggcgctcccg ctcgaccagc cgctcctgcg cttcgccggc cgccgcgcgc
     2581 ccgatggcgg cgccccggcg atccgcgcgg cctgggacga ggcgatgaga ggcaagatca
     2641 cccgctcggg caccagcgcg cccgccgaat tcgcctatac cgatttcggc cccgccggcc
     2701 gcgtcctgtc cgatacccgc gagccggccc tcggcatccg cgagatccgc tttgccaatg
     2761 gcgtgcggct caacctcaag cagaccggtc tcgaacagga ccgcgtcctc gtccagctca
     2821 gcctcgacgg cggggacatg ctggtcaccc atgacgatcc cctcgccacc gagatgatga
     2881 gcgcctttgc cgccggcggt ctcggccagc acagccagga ccagctccag tcgatccttg
     2941 ccgggcgcac ggtcggcgtc gatctccagt cgggcccgga aaccttcacc gcgatcagcc
     3001 agaccacgcc cgccgatctc gaactccagc tccagctgat ggccgcctac gtggtcgatc
     3061 cgggctaccg cccagagggc gagacgatct accgccagaa cgtcaacaac ttcttcgccc
     3121 agcgcttcgc cacgcccggc tcggcgctgg gctcttcgct cggcggcatc ctttccgaca
     3181 acgacccccg cttcaccttc cagaaggtgg aagattaccg cgcgctcacc ttcgccaagc
     3241 tcaagggcga gatatccgac cgcctgcgcc ggggggcgat cgagatcggc atagtcggtg
     3301 atattcccga ggacgaggcc attgccctcg tcgcccgcac cttcggcgcg cttccgccgc
     3361 gcgaggccga attcctgccc tatgccgagc agcacgagcg ccccttcacc cgcgatttgt
     3421 cgcgccgcat cgtccgccac accggcccgg ccgaccagtc catcgtccgc ttcacctggc
     3481 ctacccgcga cggcgaggac ccggtcgccg ccatgaagct cgaactgctc gagaaggtca
     3541 tgcgcatcga actgatcgaa accatccgcg aaaagctcgg caaggcctat agcccggcgg
     3601 ccgacagcga gaccagccgc acctggcgcg gctatggcac cttcgccgtc gccgcctcgg
     3661 tcaacatcgc cgaccttgcc gccacccgcg ccgcgatcct cgagaccgtg gccgaactgc
     3721 gcgatcgccc cgtcagcgcc gacgtgatcc agcgcgcgcg ggaacccatg ctcgaggcgc
     3781 tcgacaacgc gttgaaaaca aatcgttcct ggctctcact cgtcgatcgc gcccagaccg
     3841 aggccgatcg gatcgatcgc cagctcaagg cccgcgagcg cctgtccgag cttaccgccg
     3901 ccgatgtcca ggcaatcgcc cgccagtacc ttgtccccga ccatgcagtg gaggtcgacg
     3961 tgatccccga aggtgctccg gcgccctgag cggggctgag gcaagtcccg caaccgatcc
     4021 gaaccaatcc ttaactcctg aaaggctagt tccacggcgg atccacaatc gactgccgtg
     4081 ccaaatggtt aactgcaccc tagcccaacc gattgctcgc gccgcgcggg ccgcgcagac
     4141 tttcctcgcg cgcttgggcc gcaacaccgc cggcaatgtc ctcgccctcg tcgccgccgc
     4201 gctcccgccg atcctcatcc tggtgggcgg cgccgtcgac atgggccgcg cctacatggc
     4261 gcagaccagc ctgcagaacg cctgcgacgc cggcgtcctc gccgggcggc gcgcccaggc
     4321 caagagcggc acctggggca cggccgaaca ggccaaggcc cagaagatgt tcgacatcaa
     4381 ctatcagggc ctcgccgcca acgctaccag caccagcttc gtgccaacga accccagcgt
     4441 cggcgtgatc agcgccaccg ccaccagcac gatcccgacc acgatcatga agatcttcgg
     4501 gaccaacacc ttcaccctta ccgccagttg cagcgccgag ttccagattt ccaacatcga
     4561 cgtgatgatg gtgctcgata ccaccggctc catggcctat tgccccgatg gctcatggtg
     4621 caattccggc gccggctcga agatcgaggg cctcaagagc gcgatccgct cgttctacta
     4681 caccatcgcc gctgccgttc cgtcgggcag cggcacccgc gtgcgctttg gcttcgtgcc
     4741 ctatagtgat acggtcaatg tcaagaacct gacctccggc gcctcgcccg atatccccca
     4801 gggctacatc gccagcgccg ccaactacca gacgcggctc tacaacttcg accaggcttc
     4861 ctacacgtcc accaccggca gcccggtggt cacgcaggaa acctatggcg gatcggccac
     4921 gcccatcgcg atcagctcgg ccgattgcga caactacggg atcaatgcct atccgaccaa
     4981 cggcggcaac ccggtcacca ccggcaccgc cccggacgac gtcatcacaa agacctactc
     5041 gaagaagtcg tggacgaaga ccggaacgat caccctctct ggctgcggca actgcaccgg
     5101 caacaacggc cagggcaacg gcaacaatgg caccaaggtc gatgtcggca cctgcgtgcg
     5161 tcaggtcagc acggtcacca ccagctacgt gctcaacggc tacaagctca attcgaccac
     5221 gccttaccgc tacacccagg ccagcctcaa cacctcgggc ctcaagtcct atgcggcggt
     5281 gaaccttgcc accgaagttt actccaactc gacggtgccg acggccggcc tctatgacat
     5341 ggtcacgctc ggctcgctgg cgggaacgac gaacatcctc aaggtcagca cgacctggaa
     5401 tggctgcatc gaggaacgct cgaccgtcaa caccctgatc agcgcggcct cgatcccgtc
     5461 aggcgccacc gatctcgacg tgctgaccgc acccacctcg gccgccgcta cccagtggaa
     5521 gccctatatc tctgacttcc agttcaaccg cggtcagaca gcaacgctgg acaccaccga
     5581 cgataaggat ccgatgacgg cctattgccc ggcgccggcc aagaacttca ccacggtcga
     5641 tacctctgca ccgactacgg tccccagctg gatcgaaacc tatcttggca cgctctacgc
     5701 ctcgggcggc acctatcacg acatcggcat gatctggggt gcgcgcctcg ccaaccccgc
     5761 cgggatcaat tccaccgtcg tcaattcggg caacgtcagt tcgatcagcc ggcacatcat
     5821 ctttctgacc gacgggatta tggagccgcg ctgtgacttc ctctattcag cctacggcgt
     5881 tgaatacctc gacaaccggg tcatgcccgc gcagcccacc agggacgccg cctgtgacac
     5941 cacgctggcc acctaccaca acggtcgcta tctcgccgcc tgccaacgcg ccaaggacat
     6001 gggctacacc atctgggtga tcggcttcgg ccaggcgatc accccggaaa tgagcgcctg
     6061 cgcggcgggc agcggcgggc aagcgatgac cgccagcagc acgacggcgc tgcaggatac
     6121 cttccgcctg atcgccagcc gcatcgccaa ccttaggctt cgctcgtgac ccaccggtcc
     6181 gaacaccatc gccgccctga cggtgcgcac ctgcgccgtc tcgcggcgga ccggagcggc
     6241 gcggctctcg tcgagttcgc catcatcatt ccggtcgtcg ccttgctcct gcttggctcg
     6301 atcgagttcg gcctcaacgt ctacctgcgc gccgtgctcg aaggggcgat gcagcaggcg
     6361 gggcgcaatt ccacgctcca gtcggcccag gccgggcaga ccgccatcga caccttcgtg
     6421 cgcgaccggg tccaggcgat cctgcccaat gccacggtcg ccttcacccg cgagaactac
     6481 tacaccttct ccaccgtcgg ccgtccggaa gatttcaccg acaccaacgg caacggcgtc
     6541 cacgatgcca acgaatgctt ccaggatgcc aacggcaaca acacctggga cgccgacggc
     6601 gcccgcaccg gcaacggcgg tgccaacgac gtggtggaat ataccgccac ggtcacctac
     6661 cccagcctga tccccggcgg caccgccatc ggcctttccc ccagcacccg gatcagcgcc
     6721 accaccctcc tcaggaacca gcccttcgcc aaccagccca gctggacctc cacgcaggtc
     6781 tgcccatgag caaccgccga tcgacccgcc tccccccggc ggaagaggtg cttccccttg
     6841 ctcccctccc gcaagcggga ggggtatccc ctggcgctcc cctccctcag gctggagggg
     6901 ctgggggagg gcaagcggcc aggcgcagcg tcggcaacat cgtcaccact atcctgcgca
     6961 acaccgccgc cgtttccgcg gtcgagttcg ccctccttgc cccgctcctg atga
//
LOCUS       DATNBV010000574         5608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2234679,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000574 DATNBV010000000
VERSION     DATNBV010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2234679"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VN627_13575"
     CDS             complement(<1..386)
                     /locus_tag="VN627_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HXD04327.1"
                     /translation="MARLRDPQSGCEWDIAQTFATIAPYTIEEAYEVADAIQREDMAA
                     LKDELGDLLLQVVFHARMAEEAGLFGFDDVAEAIAAKMEARHPHVFGDATSHEANREV
                     RWEKLKAAERAAKGATSALDGVALALP"
     gene            complement(538..732)
                     /locus_tag="VN627_13580"
     CDS             complement(538..732)
                     /locus_tag="VN627_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04328.1"
                     /translation="MGGGALDGDTVVALLGVVMAMVLVSRNTRMRAMPGRSKLMLGAA
                     WAVIFGVVAGIAALLSRGSP"
     gene            complement(734..1516)
                     /locus_tag="VN627_13585"
     CDS             complement(734..1516)
                     /locus_tag="VN627_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXD04329.1"
                     /translation="MKLIMLGSGTSTGVPRIGGPDGRGDWGECDPNEPRNRRSRVSIV
                     VESSAGSRILVDTTPDLRRQLLDNAIDQVDAVFWTHDHADHTHGIDDLRPMRYGRGGP
                     LPGYGHEETVRRLRNRFGYVFAGQHGYPTICTIEPLDRLRMCAGFGVGWCQMAHGPAQ
                     STAFRFDADGKSVGYATDFSAINREMVELFDGADVLVIDCLKREPHPTHAHLAMSLEL
                     AEAARVRTAVLTHMDRSMDYAAVSSEVPGHVLVGYDGLVIEA"
     gene            complement(1513..2289)
                     /locus_tag="VN627_13590"
     CDS             complement(1513..2289)
                     /locus_tag="VN627_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXD04330.1"
                     /translation="MLVDSHCHLNYKGLVEDQQAVIARARAAGVGAFLNISTRRREWD
                     EVIGVAEREPDVWASVGIHPHEADQHADLGAETLLEAAAHPRVVAIGETGLDYYYDKS
                     DRQVQQDLFRVHVGVARETGLPLIVHTRDAEDDTARILTEEMEKEPYPALIHCFTASA
                     DFARAMLDLGLTISLSGIVTFKNAKDLQDIAKVIPDDRLLVETDAPFLAPVPHRGRPC
                     EPAFVADTARFVADLRGVSLEALSETTTANFKRLFAKAFA"
     gene            complement(2289..3869)
                     /gene="metG"
                     /locus_tag="VN627_13595"
     CDS             complement(2289..3869)
                     /gene="metG"
                     /locus_tag="VN627_13595"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HXD04331.1"
                     /translation="MAEPFYITTAISYPNGKPHIGHAYEAIAADVIARFQRLSGRDVR
                     FQTGTDEHGLKMAQKARDLGTTPQALADEMSQHFIDMCDALNVSYDVFIRTTEPRHHA
                     ATQELWRRMEANGDLYLDRYEGWYSVRDEAYYDESELVAGEGAEKLSPQGTPVEWTVE
                     ESWFFRLSKYAEPLLALFADHPEFLQPDSRRNEIMRFVEGGLRDLSVSRTSFDWGIKV
                     PGSDASGNTGHVMYVWVDALTNYLTGLGFPEETEAMAKWWPADLHLIGKDIVRFHSVY
                     WPAFLMSAGLPLPKQVFGHGFLLNRGQKESKSLGNVTDPLALAGQFGVDTLRYFLMRE
                     VAFGQDGSYSPEAIVTRANAELANSFGNLVQRVCSMIFKNMDGKLVAFTEAEADKVLL
                     GQVHEACAGELPREFAALNFSVGIEAWMRGVFACNAYIDEQAPWGLKKTDPERMGAVL
                     LTLFMAIRDLTIAISPVVPTAAGKVLDQLGIPADERNLAALADREWYLRRVATGEPLG
                     APSPAFPRLELPAEEEPA"
     gene            complement(3903..4856)
                     /locus_tag="VN627_13600"
     CDS             complement(3903..4856)
                     /locus_tag="VN627_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HXD04332.1"
                     /translation="MTLIGHDEAWREWRGAMASGRMHHAWILAGKRGLGKARFAMAAA
                     RELVAEPGLPQPEHHPDILELTPLPATDDDQKKKEAGQPYQTKRNISVDQVRQIQHRL
                     TTRPTLGARRAIIIDPADDMEKGAVNALLKSLEEPPAGTFFLLVAHRPGRLLPTIRSR
                     CRSLRFPALADGEVAHIVRTITPETDSASRAAAVAAAEGSPGAALGFVEQELGPVHAL
                     MEQIVGEGDPGFILRGALASEIGARPDRSRLLATLDLARAVLAARLRVAAPVQRRCII
                     DAHAALATLTAQVPTFNYDAGLLAMEIGGLLASAAMPREAA"
     gene            complement(4853..5482)
                     /gene="tmk"
                     /locus_tag="VN627_13605"
     CDS             complement(4853..5482)
                     /gene="tmk"
                     /locus_tag="VN627_13605"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HXD04333.1"
                     /translation="MKERGRFIAFEGGEGAGKSTQARLLAEALRARGLDVVVTREPGG
                     TAGAEAIRALLLDTAGEGFLPRAEALLFAAARADHVERLIRPALAAGQWVVCDRFLDS
                     SRAYQGGGGGLADAELLELHRIGSEGLMPDLTLLIEVSPEEAAARIAHRDGDEADRIG
                     GRPAEYHAKVAAAFRRFAEADPARFTAIDGNGPREATQAAILAAVDRLA"
BASE COUNT          947 a         1890 c         1850 g          921 t
ORIGIN      
        1 ggcagggcca aggcgacacc gtccagcgcc gatgtcgcgc ccttggccgc gcgctcggcg
       61 gccttgagct tttcccagcg aacctcgcgg tttgcctcgt gactggtggc atcgccgaac
      121 acgtgcgggt ggcgagcctc catcttggcg gcaatggcct cggcaacgtc atcgaagccg
      181 aacaggccgg cttcctcggc catccgggcg tggaacacga cctggagaag gagatcgcca
      241 agctcgtcct tgagcgccgc catgtcctcg cgttggattg cgtcggccac ttcgtaggct
      301 tcctcgatcg tgtaaggcgc gatcgtggcg aaggtctggg caatgtccca ttcgcatccg
      361 ctttgcggat cgcgcaatcg cgccatgatt gccagcaatc gggacaacga gggcgtggct
      421 gtcgcttgag gctgctcggc tgaggaaggg gggctcgcct tggaggtcat ccggttggct
      481 ccattggaat gataatatat attatgttaa atgaaaggac ctcgggtaag cgccctctca
      541 cgggctcccc cggctcagca aggcagcaat cccggcgacc acgccgaaga tcaccgccca
      601 ggcagcgccg agcatcagct tgctgcgccc aggcatggcc cgcatccgtg tgttgcggct
      661 gaccaggacc attgccatga caactccgag cagcgcgacg accgtgtccc cgtcaagcgc
      721 gccgccgccc acgtcaggcc tcgatcacca ggccgtcgta gccgaccagc acgtgaccgg
      781 gcacctcgct gctgaccgcg gcatagtcca tcgaccggtc catgtgtgtc agcacggccg
      841 tgcgcacccg ggcggcttcc gccagctcca gcgacatcgc caggtgcgca tgggtcggat
      901 gcggctcgcg cttgaggcag tcgataacga gcacgtcggc accgtcgaac agttcgacca
      961 tctcgcgatt gatcgcgctg aagtccgtgg cgtaaccaac tgatttcccg tcagcatcga
     1021 agcgaaaggc cgtgctctgc gccggcccat gggccatctg gcaccagccc acgccgaacc
     1081 cggcgcacat ccgcaaccgg tcgagcggct cgatcgtgca gatcgtcgga tagccgtgct
     1141 gtccggcgaa cacatagccg aaccggttgc gcagccgccg cacggtttcc tcgtggccat
     1201 agcccggcag cggaccgccg cggccatagc gcatgggcct gaggtcatcg atgccgtggg
     1261 tgtgatcggc atggtcgtgc gtccagaaca ccgcgtcgac ctggtcgatg gcattgtcca
     1321 gcaattggcg gcgcaggtcc ggcgtggtgt cgacgaggat gcgcgatccc gcgctgcttt
     1381 cgaccacgat ggaaacccgg ctgcggcggt tgcgcggctc gttcgggtcg cattcgcccc
     1441 agtcgccgcg cccgtccggc ccgccgatcc gcggcactcc ggtcgaggtg cccgagccaa
     1501 gcatgatcag cttcatgcga aagccttggc gaacagacgc ttgaagttcg ccgtggtggt
     1561 ttcggacagt gcctccaggc tcacgccgcg caagtccgcc acgaagcggg ccgtatccgc
     1621 cacgaaggcc ggctcgcacg gcctgccccg atggggcacc ggcgcgagga acggcgcatc
     1681 ggtttccacc agcaggcgat catccggaat taccttggca atatcctgca agtccttggc
     1741 attcttgaaa gtcacgatgc cggaaagcga gatcgtcagc cccaggtcaa gcatcgcgcg
     1801 ggcaaagtcg gccgaagcgg tgaagcagtg gatcagggcc ggataaggct ccttctccat
     1861 ctcctcggtg agaatccgag cggtgtcgtc ctcggcgtct cgagtgtgga caatcagagg
     1921 caggccggtc tcccgcgcca cgccgacgtg gacgcggaac aggtcctgct gcacctgccg
     1981 gtcgctcttg tcgtaatagt agtcgaggcc cgtctcgccg atcgcgacca cgcgcggatg
     2041 cgctgcggcc tccagcagcg tctcggcgcc aagatcggca tgctggtccg cctcatgcgg
     2101 gtgaatgccg acgctggccc agacatccgg ctcgcgctcg gcaacgccga tcacctcgtc
     2161 ccattcccgg cggcgggtgg aaatattgag gaacgccccc acccccgcgg cccgcgcccg
     2221 cgcgatgacc gcctgctggt cctcgaccag gcccttgtaa ttgagatgac agtgggaatc
     2281 gacgagcatc aggccggctc ttcctccgcc ggcagttcga gccggggaaa cgccgggctc
     2341 ggcgcgccga gcggttcgcc agtggcgacg cggcgcagat accactctct atccgcgagg
     2401 gcggcaaggt tgcgctcgtc agccggaatt cccagctggt ccaggacctt gccggccgct
     2461 gttggcacga ccggcgagat cgcgatggtt aggtcgcgga tagccatgaa cagggtaagc
     2521 agcaccgcgc ccatgcgttc cgggtcggtc ttcttcaggc cccatggcgc ctgctcgtcg
     2581 atatacgcat tgcaggcgaa gacaccgcgc atccacgcct cgatgccgac ggagaagttc
     2641 agcgccgcga actcacgcgg gagttcgccg gcgcaggctt cgtgcacctg tcccagcaga
     2701 accttgtccg cctcggcctc agtgaacgcg acaagcttgc catccatgtt cttgaagatc
     2761 atggaacaaa cccgctgcac caggttgccg aaggagttcg ccagctcggc attagcgcgg
     2821 gtcacgatgg cttcgggcga gtacgatcca tcctgcccga aggcgacctc gcgcatcagg
     2881 aaatagcgca aggtatcgac cccgaactgc ccggcgagcg ccagcggatc ggtgacattg
     2941 ccgagcgact tcgattcctt ctgcccgcga ttcagcagga agccatggcc gaacacctgc
     3001 ttgggcagcg gcagcccggc gctcatcagg aaagccggcc agtagaccga gtggaagcgg
     3061 acgatgtcct tgccgatcag gtgcagatcg gccggccacc acttggccat ggcctcggtc
     3121 tcttccggaa aaccgagacc ggtcagatag ttggtcagtg catcgaccca gacgtacatc
     3181 acgtgcccgg tattcccact ggcatcgctg ccgggaacct tgatccccca gtcgaagctg
     3241 gtccgcgaaa ccgaaagatc gcgcaggccg ccctcgacga agcgcatgat ctcgttgcgg
     3301 cggctgtcgg gctggaggaa ttccgggtgg tcggcaaaga gcgccagcag cggctcggca
     3361 tacttcgaca gccggaaaaa ccacgattcc tcaacggtcc attccactgg cgtcccctgc
     3421 gggctgagct tttcagcgcc ctccccggca accagttcgc tctcgtcgta ataggcttcg
     3481 tcgcggaccg agtaccaacc ctcgtagcgg tcgagataga ggtcgccgtt ggcttccatg
     3541 cgccgccaca gctcctgcgt cgcggcatgg tggcgcggct cggtggtgcg aatgaataca
     3601 tcgtaagaga cattcagagc gtcgcacatg tcgatgaaat gctgtgacat ttcatcggca
     3661 agcgcctggg gcgtggtacc aaggtcgcgc gccttctgcg ccatcttgag gccgtgctcg
     3721 tcggtcccgg tctggaagcg cacgtcacgg cccgagaggc gctggaagcg cgcgatcacg
     3781 tcggcggcga tcgcctcata ggcatggccg atgtgcggct tgccgttggg gtaggagatc
     3841 gctgtcgtaa tgtaaaaagg ttcggccata atcgccaatc gggtctcgcc agtcagggaa
     3901 aatcaggcgg cctctctagg catggcggcc gaggcgagca acccgccgat ttccatggcg
     3961 agcagccccg cgtcgtaatt gaaggtcggc acctgcgcgg tcagcgtggc cagggccgcg
     4021 tgggcgtcga taatgcaacg gcgctgcacc ggcgctgcaa cgcgcaggcg tgccgccagc
     4081 acggcccggg caagatccag cgtggccagc agccgcgacc gatccggccg cgcgccgatt
     4141 tcgctagcca gggcgccgcg caggatgaac cccgggtccc cctccccgac gatctgttcc
     4201 atcagggcat gcaccggccc cagctcctgc tcgacgaagc cgagcgccgc gccgggagaa
     4261 ccttccgccg ccgccaccgc cgcggcgcgg gaggcggaat ctgtttcggg ggtgatggtc
     4321 ctgacgatgt gagccacttc gccatccgcc agcgcgggaa accgaagaga gcggcagcgc
     4381 gaacggatag tggggagcag gcggcccggg cggtgggcaa ccagcaggaa gaaggtgccc
     4441 gcaggtggtt cctccaggct cttgagcagg gcgttgaccg cgcccttctc catgtcatcg
     4501 gccggatcga tgatgatcgc gcgccgcgcg ccgagggtcg gccgggtggt cagccggtgc
     4561 tggatctggc ggacctggtc gaccgagatg ttgcgcttgg tctggtaggg ctgcccggcc
     4621 tccttctttt tctggtcatc gtccgtggcg gggagcgggg tgagttcgag aatgtcgggg
     4681 tgatgctcag gctgcggcag gccgggttcg gcgaccagct cgcgggcggc ggccatggca
     4741 aagcgcgcct tgcccaggcc ccgctttccg gcaaggatcc aagcgtggtg catccggcct
     4801 gaagccatgg ccccgcgcca ttcgcgccag gcttcatcgt gtccgatgag cgtcatgcca
     4861 ggcggtccac ggcggcaagg atcgccgctt gggtcgcctc gcgcgggcca ttgccgtcga
     4921 tcgcggtgaa acgcgccggg tcagcctcgg caaaccggcg aaacgctgcg gccaccttgg
     4981 cgtggtactc ggccgggcgc ccgccgatcc ggtcggcctc gtccccatcg cggtgcgcga
     5041 tccgcgcggc ggcttcctcg gggctgactt cgatgagcag cgtcaggtcg ggcatcaggc
     5101 cctcgctgcc gatacggtgc aattcgagca gctcggcatc ggccagaccg ccgcccccgc
     5161 cctggtaggc gcggcttgaa tcgaggaacc ggtcgcagac cacccattgc cccgccgcca
     5221 gcgccgggcg gatcagccgc tcgacgtgat cggcgcgggc ggcggcaaac agcagggcct
     5281 cggcacgcgg gagaaatccc tcgcccgccg tatcgagcag cagggcgcgg atcgcctcgg
     5341 cccccgccgt gccgcccggc tcgcgggtga ccacgacatc gagcccccgg gcgcgcaggg
     5401 cctcggccag caggcgggcc tgggtggact tgccggcccc ttccccgcct tcgaaggcaa
     5461 taaaacgccc gcgttctttc acgagaccag tcccgccagg ccattgagca ggcggcggaa
     5521 agttccggcc ttggccacgt cggccccggc aaccaatggg acccgctcgg ccgggccgtt
     5581 gccgaccttg atctccagcg acgcgaca
//
LOCUS       DATNBV010000575         3679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2535596,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000575 DATNBV010000000
VERSION     DATNBV010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535596"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /gene="mutM"
                     /locus_tag="VN627_13610"
     CDS             <1..661
                     /gene="mutM"
                     /locus_tag="VN627_13610"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HXD04334.1"
                     /translation="MGRRAKYGLVHVDRGQTMVFHLGMSGRWRIDPEELEKHDHLVLE
                     TGSGHRLALCDPRRFGSVDLVATAALEQWPPFAALGPEPLSDALTPGHLAASFKDRVA
                     AIKLLLLDQRIVAGLGNIYVCEALYRARIDPRKAAGKASRPALGRLVPAIKTVLDEAI
                     AAGGSTLRDYAQPDGQLGYFSKTFDVYDREGEPCRSCSGTVRRIVQGGRSTWFCPSCQ
                     K"
     gene            982..1245
                     /gene="rpsT"
                     /locus_tag="VN627_13615"
     CDS             982..1245
                     /gene="rpsT"
                     /locus_tag="VN627_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXD04335.1"
                     /translation="MANTPQARKRIRRNEKRAEINGARIGRIRTFVKKVEAALAGGDK
                     SAAADALKAAQPELARGVARGVLHKNTASRKLSRLSKAVAKLA"
     gene            1838..3280
                     /gene="dnaA"
                     /locus_tag="VN627_13620"
     CDS             1838..3280
                     /gene="dnaA"
                     /locus_tag="VN627_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXD04336.1"
                     /translation="MMEDQEALDLAADWADISQGLRKDLGQQLHSQWIKPIQLGSFCK
                     ETGTLDLFLPTDFSANWVADRFADRLSLAWKIARSEVRHVRISVHPARRQMPDLRLGG
                     GRRPANDGAPSAPVPEPGPLTLPSADLSGSEFVTLGQPSIGLDPSLTFTSFVTGAGNV
                     LACNAAKRMAAAEKPQFSPLYLKAATGQGKTHLLHAIGHAFLAAHPRSRIFYCSAERF
                     MVEFVQALRQNQMLEFKSRLRGFDLLMVDDIQFIIGKASAQEELLYTIDALLNEGKRL
                     VFAADRAPQALDGVEQRLLSRLSMGLVADIQPADIELRRSILEHRLARFAPTDVPADV
                     VEFLARTINRNVRELVGGLNKLIAYAQLTGQKVSLQLAEEQLTDILSANRRRITIDEI
                     QRTVCQFYRVDRTEMASKRRARAVVRPRQVAMYLAKVLTPRSYPEIGRKFGGRDHSTV
                     IHAVRLIEDLRARDADMDGDVRTLLRQLED"
BASE COUNT          620 a         1260 c         1224 g          575 t
ORIGIN      
        1 catgggccgc cgcgccaagt acggcctcgt ccatgtcgac cgcggccaga ccatggtctt
       61 ccacctcggc atgtcgggcc gctggcggat cgatccggaa gagttggaga agcacgacca
      121 tctcgtgctg gagacaggct cgggccatcg cctcgcgctg tgcgacccgc ggcggttcgg
      181 ttcggtcgac ctggtggcga ccgccgcgct ggagcaatgg ccgcccttcg ccgcgctggg
      241 gccggagccg ctctccgacg ccctcacccc gggccacctt gccgcctcgt tcaaggaccg
      301 ggtggcggcg atcaagctgc tgctgctcga ccagcggatc gtggcggggc tgggcaacat
      361 ctacgtgtgc gaggcgctgt accgggcgcg gatcgatccg cgcaaggcgg cgggcaaggc
      421 ttcccggccg gcgctggggc ggctggtgcc ggcgatcaag accgtgctgg atgaagccat
      481 cgccgcgggg ggcagcaccc tgcgcgacta tgcccagccg gacgggcaac tggggtattt
      541 ctccaagacc ttcgatgtct atgaccgcga gggggagccc tgccgatcat gcagcggcac
      601 ggttcggcgg atcgtccagg gcgggcgcag cacctggttc tgcccgagct gccagaagta
      661 ggggagcggg cgctccctgc tatcaggggc gcttgccgcg agtcttttgt tgggtgcgga
      721 aagcgcatcc gcgctttcct tgcggcaccg gcccgctccc ccggcccaac cacccacagg
      781 atacactcaa tgggtggttg ggccggggga gcgggcgggt gccgcaagcc ctcggcggat
      841 gtcggggcag cactaatcaa gattgacgat tcgccccggc gcggttaagg gccgcgcttc
      901 ccgcggggcg gcttcggctg aactccgcga ttttgcacgc aattcaggac agggccggga
      961 ccggccgaac gaaggacaga catggccaat acgccgcaag cccgcaagcg catccgtcgc
     1021 aacgagaagc gcgccgaaat caatggcgcc cgcatcggcc gcatccgcac cttcgtgaag
     1081 aaggtcgaag cggccctggc cggtggcgac aagtccgctg cggctgacgc gctcaaggcg
     1141 gcccagccgg aactggctcg cggcgtggcc cgcggcgtgc tccacaagaa cactgcctcg
     1201 cgcaagctgt cgcgcctgtc gaaggccgtc gccaagctcg cctgagcagg cgaatcgatt
     1261 cgccaccgga caggccccga gggcctttcc ggtgggacga aaacggaaca cggccggggg
     1321 tcatcctccg gcctttttcg tggccgggta aatgcctccc ggggcacctc gctcaggtgc
     1381 tttagaaaag ggcagaattt ctgcgattcg gctgcgcggt tgagcaaaaa cgacggaaaa
     1441 cagcactttg atacgtaaac aacgggattc tccggggtcc cctagtgtca aggagcatta
     1501 tttcagtttt tttgcagccg ggcgcttgcg cggcgccaaa tccgcttcat aaatcgccct
     1561 gccccgagcg gcagcgcaaa ggggtatcac gggacatgta tcgaaggacg cgccgggcac
     1621 accaggtccc cggcaagggt cgagccgtgt ctgctgcaag cgggggcgcg cagccaagtg
     1681 gtgggggttc tgacaggatg ggtattccga caggacagcg gcaactggca cggaccgggc
     1741 acggcgcagc agcgcccgta ctcgatccgt cgccaagggt tttcgggcgc aggggtttgt
     1801 tggcatgaca cataagggca agatcagcga agcgcgtatg atggaagacc aggaagccct
     1861 cgatcttgcc gccgactggg cggatatcag ccagggcctg cgcaaggacc tggggcagca
     1921 gctgcacagc cagtggatca agccgatcca gctgggctcc ttctgcaagg aaacgggtac
     1981 gctcgacctg ttcctgccga ccgacttctc cgccaactgg gtggccgacc ggtttgccga
     2041 ccggctgagc ctggcctgga agatcgcccg ttcggaagtg cgccacgttc gcatctcggt
     2101 tcaccccgcc cgccggcaga tgcccgacct gcgccttggc ggcggtcgtc gcccggccaa
     2161 cgatggcgcc ccctcggcac ccgtgccgga gcccggcccc ctcaccctgc cctcggccga
     2221 cctgtcgggc agcgaattcg ttaccctggg ccagccgtcg atcgggcttg acccctcgct
     2281 gaccttcacc tcgttcgtga ccggcgccgg caacgtgctg gcctgcaatg ccgccaagcg
     2341 catggcggcg gcggaaaagc cgcagttctc tccgctctac ctcaaggcgg ccaccggcca
     2401 gggcaagacc cacctgctcc atgccatcgg ccacgccttc ctcgcggccc atccgcgcag
     2461 ccggatcttc tactgctcgg ccgagcggtt catggtcgaa ttcgtccagg ccctgcgcca
     2521 gaaccagatg ctggaattca agtcccgcct gcgcggcttc gacctgctga tggttgacga
     2581 catccagttc atcatcggca aggcttcggc ccaggaagag ctgctctaca cgatcgacgc
     2641 cctgctcaac gagggcaagc ggctggtctt cgcggccgac cgcgcgcccc aggcgctcga
     2701 cggggtggag cagcgcctgc tctcgcgcct gtcgatgggc ctcgtggccg atatccagcc
     2761 ggccgacatc gaactgcgcc gctcgatcct cgagcatcgc ctcgcccgct tcgcgccgac
     2821 cgacgtgccg gccgacgtgg tcgaattcct cgcccggacc atcaaccgca atgtgcgcga
     2881 gctggtcggc ggcctcaaca agctgatcgc ctatgcccag ctgaccggcc agaaggtgag
     2941 cctgcagctt gccgaggagc agctgaccga catcctctcg gccaaccgcc gccggatcac
     3001 gatcgacgag atccagcgca cggtctgcca gttctaccgg gtcgaccgga cggaaatggc
     3061 gagcaagcgc cgggcccgcg ccgtggtccg cccgcggcag gtggcgatgt acctggccaa
     3121 ggtactgacg ccgcggtcct acccggagat cggccgcaag tttggcggac gcgatcactc
     3181 cacggtgatc cacgccgtgc gcctgatcga ggacctgcgc gcccgcgacg ccgacatgga
     3241 cggcgacgtg cgcaccctgc tgcgccagct ggaagactag ggtggcggtt gcgggcgtct
     3301 gacagtctcg ttcgccccgc tcacaccgcc gatagcatcc cgtggcttaa aggaagccgg
     3361 tcgcacggcg cccagcggat atacaccgcg gcgacaccgg ccctgcccgg gttatcccct
     3421 gccttgtcaa cagaccgcct gcggccagat gggtcaaaga cgtgaaaaag gcccccgcct
     3481 tgcggcggag gccctgtcgc gcgcttcagg cggtccctag aaccgcagcc cgatcatcga
     3541 gcccacgccc acatcgggcg cgcccttggt aaggccggcc gagccgtaga gcgtgaagtt
     3601 gaggcggtgc gagaccggcg tgctgaaggc gccgaacagt tcctcgctat cgcgcgagag
     3661 atcgctggtg gcctggcga
//
LOCUS       DATNBV010000576         2541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4440004,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000576 DATNBV010000000
VERSION     DATNBV010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4440004"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2541)
                     /locus_tag="VN627_13625"
     CDS             complement(<1..>2541)
                     /locus_tag="VN627_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HXD04337.1"
                     /translation="IAAAAVLALFAILFGARRFELAGRSEGLVFAIGLESALKLLALL
                     AVAGLATVLFLHAGPARQADGFAQIAERFNPTHLSLDTVAIAMIAALAIIALPRQFYM
                     GLVEAREPEDLVRARGGLAAYLAVMAALAIPIALAGAALFDGVVAPDVFVLELPLEAG
                     AHWALVAALLGGVGAAASMVIVDATALATMVSNDLVFPSILRTAQAGGASAPAGGSIG
                     RRMLLVRRLSIVGIVALALAWAALVGRTNSLASIGHVAFAAMALFAPHLLLAAARGGR
                     DPLPARVSLAAGLVLWLWTLALPPIMPEAWLAALRGTLIDPLNLLGIGKATPLVHGVG
                     WSLGVDVGLLGVVAARRGAGPARISLFAPGRPVSDLAGLHRLVADFVGTEAAEREFPA
                     ARHGDPVDRAAAQRAQELIARVVGASSARTLVASALAGGQMRLADVARLLDEGAQSLR
                     FSRPLLAATFENVDTAISVVDAEMNLVAWNTRYLDMFDYPPGLARVGVPVADLIRHNA
                     KAGDFGPEPVEHHVAKRLAHMRRGQPHEFERRRRDGRVIKTVGGPMPGGGYVMSFTDV
                     TAEAEARDELQRTLAELESRVAERTRELSEANRQLAEATRDKTRFLAAASHDLLQPLH
                     AARLFAAALARDPGLTRPELVQRLDRSILGGENLLRALLDISKLDAGGVQPACSAVAL
                     RPFLADLVDGIRPLAAERGLALRLGPTPGSLFTDPGLLRSVLQNFLTNAVRYTTRGGI
                     LVGVRRRGDNWRIDVVDTGIGVPPARQAEIFKEFIRIGEAEAEGLGLGLAIATRIVRL
                     LGGRIELVSAPGKGSRFSLLLPAAPETTAPPAPISAAPGEARSMAVLVI"
BASE COUNT          424 a          841 c          969 g          307 t
ORIGIN      
        1 gatcaccagc acggccatgg accgcgcctc gccgggcgct gcggaaatgg gcgccggcgg
       61 cgcggtagtt tccggggcag cgggtagcag gaggctgaaa cggctgccct ttccgggagc
      121 tgataccagt tcgattcgcc ctccgagcag gcggacgatc cgggtggcga tggcgaggcc
      181 aaggccgagc ccttcggctt ccgcttcgcc gatacgaatg aattccttga agatttctgc
      241 ctggcgcgca ggcgggacgc cgatgccggt atccaccaca tcgatccgcc aattgtcccc
      301 gcgccgccgg acgccaacga gaatgccgcc gcgggtggtg taacgcacgg cattggtcag
      361 gaaattctgg aggacggagc ggagcaggcc ggggtcggtg aacaggctgc cgggggtggg
      421 ccccagccgc agggcaagac cgcgctcggc ggcgagcggg cggatgccgt cgaccagatc
      481 ggccagaaag gggcgcagtg caacggcgct gcaagcgggc tgcacgccgc cggcatcgag
      541 cttggagata tcgagcaggg cccgcagcag gttctcgccg ccgaggatcg agcggtcgag
      601 gcgctgcacc agttcgggcc gggtcagtcc tgggtcgcgc gcgagggcgg cggcgaagag
      661 gcgggcggcg tgaagcggct ggagcaggtc gtgactggcg gcggcgagga agcgggtctt
      721 gtcgcgcgtg gcttcggcga gctggcggtt ggcctcggac agttcgcggg tccgttcggc
      781 gacccggctt tcaagttcgg caagggtgcg ctggagttcg tcgcgggctt cggcctcggc
      841 ggtgacgtcg gtaaagctca tgacgtagcc gccgccgggc atcgggccgc ccacggtctt
      901 gatcacccgc ccgtcgcggc ggcggcgctc gaactcgtgg ggctggcccc ggcgcatgtg
      961 ggcgaggcgc ttggcgacat ggtgctcaac cggctcgggc ccgaaatcgc cggccttggc
     1021 attgtggcgg atgagatcgg caacggggac gccgacccgg gccagtcccg gcggatagtc
     1081 gaacatgtcg agatagcggg tgttccacgc gacgaggttc atctcggcat cgaccacgct
     1141 gatcgccgta tccacgttct caaaggtggc ggcgagcagg gggcgcgaga agcgcagcga
     1201 ctgggcgccc tcgtcgagca ggcgggcaac gtcggcaagc cgcatctggc cgccggcgag
     1261 agccgaggcg acgagggtgc gggccgagct ggcgccgacc acccgcgcga tcagttcctg
     1321 cgcgcgctgg gccgcggcac ggtccaccgg atcgccgtgg cgggcggcag ggaattcgcg
     1381 ctcggccgct tcggtgccga cgaaatcggc gacgaggcga tggaggccgg caaggtcgct
     1441 gaccggtcgg cccggcgcga acaggctgat ccgcgcggga ccggcgccgc gcctggcagc
     1501 caccacgcca agcaggccaa catcgacccc gaggctccag ccgacgccgt ggaccagcgg
     1561 tgtggccttg ccgataccca gaaggttgag cggatcaatg agagtgccgc gcagggccgc
     1621 cagccaagcc tcgggcatga tcggcggcag ggccagcgtc cacagccaca gcacgagccc
     1681 ggcggcgagg ctcacccggg ccggcaacgg atcacgcccg ccgcgggctg ccgccagcaa
     1741 caggtgcggc gcgaacaggg ccatggcggc aaaggcgacg tgaccgatcg aggcaagcga
     1801 attggtgcgg ccgaccagcg ccgcccaggc gagggcaagg gcgacaatgc cgacgatcga
     1861 caggcgccgg acgaggagca tgcgccggcc gatgctgccg ccggccgggg cgctcgcccc
     1921 gcccgcctgt gccgtccgca gaatcgaggg gaaaacgagg tcattcgaga ccatcgtcgc
     1981 cagcgccgtc gcatccacga tgaccatcga ggccgccgcg ccgactccgc cgagcagggc
     2041 cgcgacgagc gcccagtgcg cgccggcctc gagcggaagt tcaagcacga acacatccgg
     2101 cgcgaccacg ccgtcgaaca gggctgcgcc ggcaagggcg atggggatgg cgagcgccgc
     2161 catcactgcg agataggcgg cgaggccacc ccgggcccgc acgaggtctt ccggctcgcg
     2221 cgcctcgacc aggcccatgt agaactggcg cggcagcgcg atgattgcca gcgccgcgat
     2281 catcgcgatc gcgactgtat ccaggctgag gtgggtagga ttgaaccgtt cggcgatctg
     2341 ggcgaagcca tcggcctggc gggctggtcc ggcgtggagg aacagcaccg tggcaaggcc
     2401 ggccacggcg agcagggcca gcagcttcag cgccgattcg aggccgatgg cgaagaccag
     2461 cccctcgctg cgcccggcca gctcgaaccg ccgcgcgccg aacaggatcg cgaacagcgc
     2521 cagcacggca gcggcggcga t
//
LOCUS       DATNBV010000577         2800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2537036,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000577 DATNBV010000000
VERSION     DATNBV010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2537036"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1250)
                     /gene="rffA"
                     /locus_tag="VN627_13630"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(105..1250)
                     /gene="rffA"
                     /locus_tag="VN627_13630"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF008687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HXD04338.1"
                     /translation="MQEIPFNRPALIGREVSYLREAIRRGQLSGDGHFTKRCNALIAE
                     MTGSGKALLTHSCTAALEMAALLCDLEPGDEVIMPSFTFVSTANAVALRRATPVFVDI
                     DPLTLNVDPARVAEAMGPRTRAIFPVHYAGVPADMDAINAIARAGDCLVVEDAAQALG
                     STYKGRPCGSLGDLAAFSFHETKNIISGEGGALTINRADLQDRAEIIREKGTNRSQFF
                     RGEVDKYTWVDIGSSFLPGELIASYLCAQLEMAELINARRLAIYDRYREAFADLAASG
                     RVALPFIPDGCTGNGHMFYLLLRDLDDRSAFIAHMRAHGIIAPFHYVPLHSAPAGQSH
                     ARSVGPMDVTERISDTLVRLPMFFELGSQVEDVIDQALAYFVGERAA"
     gene            complement(1284..>2800)
                     /locus_tag="VN627_13635"
     CDS             complement(1284..>2800)
                     /locus_tag="VN627_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04339.1"
                     /translation="RMFGAVVDRHALWIYGTLLVLLFAVCQLAVRSFTVETDEAWILL
                     STFKALRLGMGGSEAVGFPTVTSGGLHFLLHGLLGRATDSIVVHRTVSLLFLAALVLL
                     VFGLMRRLGAEPRHAAAGGMLVLATPGLVLQASLAMAEIIATVVLLATVVYWERRGRL
                     SLSGSAITGLLLGLTCATRTNCLVSIPVMAVCATLDASDWTTGLSRAAAMVAGSVAVY
                     GIGVGGYAGLFVVGNWAGFAHVMGGATGASVDKNLGDYLLNLNLAADFFPPLLIAGVL
                     AGLVAIWRRGTGDLRLPLVLVSISLVGALAWIIGAPIPHVRYAWPFAPFLFVAAGMLA
                     HLTGALARPWSQFIVHLFVIGSVLHQTVTSLVYIGAGDSLVAVSQPSRQVGIPSQLEH
                     TTNIRQQEDIARFIAALPAQADITTEFYGTGFPLSYLSQRPVLGIAPSDSPRLRPLFF
                     IIAPGDIAVPHQHSRVIGWLQRNCTLVFRAGDYAIFSVHGPEPFPVLVAVHQAG"
BASE COUNT          556 a          891 c          938 g          415 t
ORIGIN      
        1 gagccctgtg ccggcaaagg cgatgcgcac ggcttcgctc aatggctcga ggcaagcggt
       61 gcagccgtag accggcgaaa ccacgctgat caggaccggc gggctcatgc cgcgcgttcc
      121 ccgacgaaat aggccagcgc ctggtcgatc acgtcctcga cctgcgagcc cagctcgaag
      181 aacatcggta ggcggaccag cgtgtcgctg atccgctcgg tcacgtccat cggcccgacc
      241 gagcgggcat gactttgccc ggcgggggcg ctgtggagcg gcacatagtg gaacggcgcg
      301 atgattccgt gggcgcgcat gtgggcgatg aacgccgacc tgtcgtcaag gtcacgcagc
      361 agcaggtaga acatgtggcc gttgcccgtg cagccatccg ggatgaaggg cagggcgacc
      421 cgcccggatg cggcgagatc ggcaaaggcc tcgcggtagc ggtcgtagat cgccaggcgc
      481 cgcgcattga tcaactcggc catctcgagt tgggcacaga ggtaggaggc gatcaactcg
      541 cccggcagga acgaggagcc gatgtccacc caggtgtact tgtccacttc gccgcggaag
      601 aactgcgagc ggttggtgcc cttctcgcgg atgatttcgg cgcggtcctg caggtcggcc
      661 cggttgatcg tcagcgcccc gccttcgccg ctgatgatgt tcttcgtctc gtggaagctg
      721 aaggccgcaa ggtcgcccag cgaaccgcag gggcgtccct tgtaggtgga gcccagggcc
      781 tgtgccgcat cttccaccac caggcagtca cccgcgcggg cgatggcatt gatcgcgtcc
      841 atgtccgccg gcaccccggc atagtgcacc ggaaagatcg ctcgcgtgcg cggtcccatc
      901 gcctcggcca ctcgcgcggg atcgacgttg agcgtgagcg gatcgatgtc gacgaacacc
      961 ggcgtcgccc gccgcagcgc taccgcattg gccgtggaga cgaaggtgaa cgagggcatg
     1021 atcacctcgt cgcccggttc gagatcgcac agcagcgccg ccatctccag cgcggccgtg
     1081 cacgaatggg tcagcagggc ctttccggag ccggtcatct ccgcgatgag ggcattgcac
     1141 ctcttggtga aatgcccgtc gcccgaaagc tgcccgcgcc ggatcgcctc gcgcaggtag
     1201 ctcacttccc tgccgatcag cgccgggcgg ttgaagggaa tttcctgcat tgcggggtgc
     1261 cttgtgaaat tagttggatc gggtcagccg gcttggtgca ctgccaccag gactggaaat
     1321 ggctccgggc cgtggaccga gaatatcgca tagtcgccgg ccctgaacac cagtgtgcaa
     1381 ttgcgctgga gccagccgat cacccgactg tgctggtggg gtaccgcgat atcgccgggg
     1441 gcgatgatga aaaagagggg gcgcaagcgc gggctgtccg acggcgcgat gccgagaact
     1501 ggccgctgcg agaggtagct cagcgggaat cccgtgccat agaattcggt cgtaatatcg
     1561 gcctgggccg gaagtgccgc gatgaagcgc gcaatatctt cctgctgccg gatatttgtc
     1621 gtgtgctcca gctggctggg aatgccaacc tgccggcttg gctgcgagac cgccaccagg
     1681 ctgtcgccgg ccccgatata gacaagagaa gtaacagtct gatggagcac cgatccgatc
     1741 acgaacaggt gaacgatgaa ctgcgaccag ggccgggcaa gggcgccggt cagatgcgcc
     1801 aacatgcccg cagcgacaaa caggaacggc gcgaacggcc acgcatagcg gacatggggg
     1861 atgggggccc caataatcca ggccagggcg ccgaccaggc tgattgatac aagcaccagc
     1921 ggaaggcgca gatcaccggt gccgcgccgc cagattgcga caaggcccgc cagcacgccc
     1981 gcgatgagca agggagggaa gaaatcggcg gcaaggttga ggttcagcag gtagtcgccc
     2041 aggttcttgt cgaccgatgc ccccgtcgca ccgcccatga cgtgggcgaa gccggcccag
     2101 tttccaacga cgaacaggcc ggcgtagccg cccaccccga taccgtagac ggcaactgat
     2161 ccggcaacca tcgccgcggc gcgggacagg ccggtcgtcc agtccgaggc gtcgagggtg
     2221 gcgcagaccg ccatgacggg aatgctcacc aggcaattcg tccgggtggc gcaagtcagt
     2281 cccagcagga gtcccgtgat cgccgatccg gacagactga gccggcccct gcgctcccag
     2341 tagacgactg ttgcaagcaa cacgaccgtc gcgatgatct ccgccattgc caggctggcc
     2401 tggagaacca gtcccggcgt ggccagaacc agcatccccc cggcggcggc atgcctgggc
     2461 tcggcaccga gccggcgcat cagaccgaac accaacagca ccagtgcggc aagaaacagc
     2521 agcgagacgg ttcggtgaac cacgatcgaa tccgttgcgc ggccgagcaa accgtgaagc
     2581 aggaagtgga gcccgccgct ggtgacagtg gggaatccga cagcttcgct cccacccatg
     2641 cccagtcgaa gggccttgaa ggtggacaac agtatccatg cctcgtcggt ctccacggta
     2701 aaggatcgca cggccaactg gcaaaccgcg aacaagagca cgaggagggt gccgtaaatc
     2761 cagagcgcgt gacgatcgac cacggcgccg aacatccgcg
//
LOCUS       DATNBV010000578         8420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2537138,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000578 DATNBV010000000
VERSION     DATNBV010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2537138"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1328
                     /locus_tag="VN627_13640"
     CDS             204..1328
                     /locus_tag="VN627_13640"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HXD04340.1"
                     /translation="MEKTEPPIVAPSQRSLDELAQTLANWLPTRIEGASDIAITDLRY
                     PRGAGLSHETILFTASWRVDGVAHERGLVVRIKPRPDKSVYLDDQFVDQFRIMRAARQ
                     SGHVRVAEVLWLEEDPAILGAPFFVMERMDGRVPVSYPSYVTHGWLAEATPAERRVAW
                     EDSVRQLAAIQLIPHQDVPFLDLGTGGTGFEQEIDRWRRYVAWITEPGPLPFVERAFA
                     EILRTAPANPPPGIVWGDARIGNMMFDDSFRVLAVMDWEQVSLGGALHDLGWWLSNEH
                     HRTVGSGVARPDGIGSAEETCALWSEVTGIPVTDIEWYTVFACFKMNCLRTHMLDANW
                     YPPTPGNDWGECPGWHFVAEHLGIAPPEPMPRRILWEDVR"
     gene            complement(1404..2174)
                     /locus_tag="VN627_13645"
     CDS             complement(1404..2174)
                     /locus_tag="VN627_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD04341.1"
                     /translation="MVGKLLAGKSVVIAGAGSGVGRAASLLFARHGARLVCADIMADW
                     VAETVRLVQAEGGEAIAVTCNVVSEADIQRAIDAAVDAYGRLDVIYNNVGITGGPVQL
                     AETSEEQFEKLVSVNFGGVVRGSKLAVRQFRKQGTGGVIVNTASVSGLIGWGGVVYGG
                     TKGAVVAMTRTLAMEVAKDGIRVNSVCPGGMITNFGADEPFATPTPEKVAAMGSFHPL
                     GRPIDPRNSAALAQSMGSDLSSNITGVNLPVDGGLSAG"
     gene            complement(2190..3701)
                     /locus_tag="VN627_13650"
     CDS             complement(2190..3701)
                     /locus_tag="VN627_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXD04342.1"
                     /translation="MEVVESEAMAALSDGGTSQAVDGLYCGQFQSGQGPLLDVVDPSD
                     GRAFAQVRTASPGQIADTIGAARSAFDSGVWSGLPIAERAAYLRRMLQRMEGQSARLL
                     DLLVREAGVVRGSMTRPIHVDVALRHGFQCIDLALTLPEAVDNPIPIAERATPNYLLE
                     SFQRYEPVGVVAAISAYNVPLFIGLWKIIPALITGNTVILRPSPLTPLATLLIGEAAS
                     AAGLPDGVLNIVAEAGNDGAVLLSTDRRIDMVTFTGSSAVGKQVIRQSADTMKRYQLE
                     LGGKSAQIYLPDALELAAGAADRVCGVQSGQGCVLGTRIFVPAAEKAALLDRMAQSVG
                     KMTVGPAEGADTVVGPVINAAAVERCEAIVAAAIAAGGRVVAGGRRPAHLTEGFYFEP
                     TILDVPDNSNPAAQEEIFGPVVCVIGYDNLDHAVAMANDTQYGLSGHVFGKDMKAAMD
                     VALRLRTGTVNINGGMISAYASSGGRKLSGFGRERGVEGIRIYQEIKCISITR"
     gene            complement(3728..4870)
                     /locus_tag="VN627_13655"
     CDS             complement(3728..4870)
                     /locus_tag="VN627_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXD04343.1"
                     /translation="MVDYKVICADSHVNPAPDFWQDYLPESLRHLAPRIEQGDDADYV
                     VFEGRRKKLNLIGAQAGRSGENFKIEGRLSDMRGGGWLPDERLKDMDQDGLDAAVMFG
                     GGPLGTTSDELYIESYDAYNRWLADFCAYEPKRLIGVAYLPMRDIDETITMMRAAAKR
                     GFKACNIPAFPQAKTLKEMDIAGGAQTLALTGNPFGELQYDDPQFDRFWAEAQDLDMT
                     ICIHLGARMARWQEPRFFLSDLLMTKLAMAEPIALLIFGGVFQRFPKLRFVSVESGVG
                     WFAFAANYMDMTWQKQRYWTKNKLTEPPSFYMDQNVYGSFIHDKVGVDTRHMPGARNI
                     MWSSDYPHSETSYPESRQCIENLFAGVPEDEKYEIICGRAKRVFGL"
     gene            complement(4972..5802)
                     /locus_tag="VN627_13660"
     CDS             complement(4972..5802)
                     /locus_tag="VN627_13660"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXD04344.1"
                     /translation="MKARFPTFDFSNVRAHWTRSPEFAQRCNAASLVPAYVEPFLLKV
                     MKTARDKIDPANTRLIEEIDIFCKQEMQHCKQHLAFNAAIRRQGYEGLKPLEDKMQAD
                     YKAFLEQRSLRFNLAYCEGFESMSASGCEAWFEDYGELLEGADPATADLWRWHLAEEF
                     EHRTVCSEVFHALSGLDPVSRYFYRIYGYAYALIHIGRFVKAAGQYLRTTDREAMSAD
                     ELAQSLAREAEVKRITTRRILPMMLKVLSPFYDPAKRREPEGYRAFLDSLEQRRATAA
                     "
     gene            6065..7129
                     /locus_tag="VN627_13665"
     CDS             6065..7129
                     /locus_tag="VN627_13665"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXD04345.1"
                     /translation="MHLTAPAPTFSPAPLVLEEVLDPAWLGMALGGIEVRDVRVVETL
                     GPSCTKVRMELAFGPGVDPAMPTAYCLKGFFGDPALGYLQSGTQLTEAAFYRDCAPHL
                     TMRLAECVHAGSDEQTGAGVILMKDLIAGGARFLTALEPYSVEQARSSVDQLARLHAA
                     SWGEAKLARWPWVRPRVEELTNYKGVPRERLTELMRGERGEPLPDELRDGQRIYAALT
                     SIAGRDAGLQHCLVHGDAHAGNVFETPEGCGMVDWQLLQRGHWSLDVAYHIGAVMTVE
                     NRRQHEESLLRHYLDRLDAHGGQAPAWDEAWDRYRESLAYGMFLWGITVRVDPPIIME
                     FNRRLGTAVADHESFRRLGV"
     gene            7227..8072
                     /locus_tag="VN627_13670"
     CDS             7227..8072
                     /locus_tag="VN627_13670"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXD04346.1"
                     /translation="MSRASPAATRVVSVINFFVEHPSDAFTLTELVKSLRLSRATCHA
                     LLATLVDKGYLYRKPDKSYVIGPALVAAGRIARENYSPLEIARAEMRGLADEFDAICT
                     AQFIEKDEMVLRDRAGGISHLGWLPPIGQRVSMNSLWAAPSMSFESDEAIAQWLDTIK
                     NEATPEQREEVRAAMDAARERGYFVTVRLDSWSVHLAQAPIAERREHTNFFVRELDDD
                     RSYQLSFILVPVMDAKGLAFVMTLSGFLHEVTGKKIKQIAETAVAASQRISRFLKHAD
                     GVSPA"
     gene            8119..>8420
                     /locus_tag="VN627_13675"
     CDS             8119..>8420
                     /locus_tag="VN627_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HXD04347.1"
                     /translation="MSAEIFPFHAVVSDEVLRDLAARLARTRYPDQLEGAGWDYGTEL
                     GYLSHFCEYWRTGYDWRAAEARFNAFPQFVTEAQGERLHFYHVRSPEPDALPLIVT"
BASE COUNT         1422 a         2781 c         2680 g         1537 t
ORIGIN      
        1 tggcgcccat gctgccctga ccagtcgaca aggcctgatc aattgaaatc ccggcatttt
       61 tccccgctgc cgcagcccga tccgcgatgc tacggcagca gtgcggcccc gccacggccg
      121 gcccgccggt tccatatatc aaactcttgg tcgttcatgc gaattgattg acatcaattg
      181 ggagtggtga cagtccagag gacatggaga aaaccgagcc gcccatcgtc gccccgtccc
      241 agcgctcgct ggatgaactc gcgcaaaccc tggcaaattg gctgccgacg cgcatcgagg
      301 gggcgagcga tatcgccatt accgacctgc gctatccccg cggcgccggc ttgtcgcacg
      361 agacgatcct gttcaccgcc agttggcggg ttgacggcgt ggcccatgag cgtggcctgg
      421 tcgtgaggat caagccccgg ccggacaagt cggtctatct cgacgatcag ttcgttgacc
      481 agttccggat catgcgcgcc gctcggcaga gcggtcatgt gcgggtggcc gaggtgctgt
      541 ggctcgagga agatcccgcg attcttggtg cgccattctt cgtgatggag cggatggatg
      601 gccgggtgcc ggtcagctac ccgtcctacg tgacccacgg ctggcttgcc gaagccaccc
      661 cggccgagcg gcgtgttgcc tgggaggatt cggttcggca actggcggcc atccagttga
      721 tcccgcacca ggacgtgccg ttcctcgatc tgggcaccgg cgggacgggt ttcgaacagg
      781 agatcgatcg ctggcgccgg tacgtggcgt ggatcaccga gcccgggccc ctgccgttcg
      841 tcgaacgtgc cttcgccgaa attctccgta ccgctccggc caatccgccg cccggcatag
      901 tctggggcga tgcccggatc ggcaacatga tgttcgacga cagcttccgc gtcctcgcag
      961 tgatggactg ggagcaggtt tcgctgggcg gcgctctgca cgacctgggc tggtggctct
     1021 ccaacgagca ccatcgcacc gtgggatcag gcgtcgcgcg cccggatggc atcgggtccg
     1081 ccgaagagac ctgcgcgctc tggtccgagg tgaccggcat cccggtcacg gatatcgaat
     1141 ggtatacggt ctttgcctgc ttcaagatga actgtctgcg cacgcacatg ctggatgcca
     1201 actggtaccc gccaaccccc ggcaacgact ggggcgaatg cccaggctgg catttcgtgg
     1261 ccgagcacct cggcatcgct ccgcccgaac ccatgcccag gcgcatcctg tgggaggacg
     1321 tccgctgaat tgaccggggc acagtgccgt gcccgatccc cggatcggaa ccgtcccggc
     1381 cttaactgtc cagctgccgg ctgttaccct gcggagagtc cgccatcgac cggcaagttg
     1441 actccggtga tgttggatga aaggtctgac cccatggact gcgccaatgc cgcgctgttc
     1501 ctggggtcga tcgggcgtcc caggggatga aagctcccca tcgcggccac tttttcgggg
     1561 gtgggcgtcg caaagggctc gtcggcgccg aaattggtga tcattccgcc cgggcagacc
     1621 gagttgacgc ggatcccgtc cttggccact tccatcgcca gggtgcgggt catggccacg
     1681 acggcgccct tggttccgcc atatacgacc ccgccccagc cgatcagccc tgagaccgac
     1741 gcggtattga cgatgacgcc gccggtgccc tgcttccgga actgccggac ggcgagcttg
     1801 cttccccgga cgaccccgcc gaagttcacg ctgaccagct tttcgaactg ttcttcgctc
     1861 gtctcggcca gttggaccgg cccgccggta atgccgacat tgttgtagat cacgtccagc
     1921 cgcccatagg catcgacggc ggcatcgatc gcccgctgga tatccgcctc gctgaccacg
     1981 ttgcaggtca cggcgatcgc ctccccgcct tcggcctgca cgagacgcac cgtctcggcc
     2041 acccagtccg ccatgatgtc ggcgcagacc agccgcgcgc cgtggcgggc gaacagcagg
     2101 gaggcggccc ggccgacccc cgacccggcg ccggctatga cgaccgactt accagcaagc
     2161 agcttcccga ccatgccttc tcccctgctt tatcgcgtta tgctgatgca cttgatttcc
     2221 tggtagatgc gaatgccctc gacgccgcgt tcgcgcccga acccgctcag ctttcgcccc
     2281 ccgctcgagg cataggcgct gatcatgccg ccattgatgt tcaccgtgcc ggtgcgcagc
     2341 cgcagcgcca cgtccatggc ggccttcatg tccttgccga agacgtggcc ggagagccca
     2401 tattgcgtgt cgttggccat ggccacggcg tggtcgagat tgtcgtagcc gatcacgcag
     2461 acgaccgggc cgaatatttc ctcctgcgcc gccggattgc tattgtcggg cacgtcgagg
     2521 atcgtgggct caaaatagaa cccttccgtc aaatgggcgg ggcgccgccc gcctgccacc
     2581 acgcggcccc cggcggcaat agccgcggcg acgatcgcct cgcagcgctc caccgcggcg
     2641 gcattgatca ccgggccgac caccgtgtcc gccccttccg ccgggccgac ggtcatcttg
     2701 ccgaccgact gcgccatccg gtcaagcaag gcggcctttt ccgcggccgg gacaaagatc
     2761 cgggtcccca gcacgcagcc ttggcccgat tgcacgccgc acacgcgatc ggccgcaccg
     2821 gctgcaagct cgagggcgtc gggcaggtag atctgggccg acttgccgcc cagttccagc
     2881 tggtagcgct tcatcgtgtc ggcgctctgc cggatgacct gcttgcccac cgcgctggat
     2941 ccggtgaagg tgaccatgtc gatcctgcgg tcggtgctga gcagcacggc gccgtcgttc
     3001 ccggcctcgg cgacgatgtt gagaacgccg tcaggcaggc cggcggcgga agccgcctcg
     3061 ccgatcagga gcgttgccag cggtgtcagt gggcttggcc gcaggatgac ggtattgccg
     3121 gtgatcagcg cggggatgat cttccacagg ccgatgaaga gcggaacgtt gtaggccgag
     3181 atcgcggcaa cgacgcccac gggctcatag cgctggaagc tctctagaag gtagttcggg
     3241 gtcgcacgct cggcaatcgg aatggggttg tcgactgctt ccggcaaggt cagggcgaga
     3301 tcgatgcact ggaaaccgtg gcgcagggcg acgtcgacat ggatcggcct tgtcatcgat
     3361 ccgcgcacca cgcccgcttc gcggaccagc aggtccagca gccgcgccga ctggccctcc
     3421 atgcgctgca gcatgcgccg cagataggca gcccgttccg ctatcggcag gccggaccag
     3481 acgccgctgt cgaaggccga gcgggcggcg ccgatcgtgt cggcgatctg gccgggcgag
     3541 gccgtgcgca cttgcgcgaa ggcgcggccg tccgatggat cgacaacgtc gagcagaggt
     3601 ccctggcctg attggaactg accgcaataa aggccatcaa ctgcctgaga cgtgccacca
     3661 tcggacaagg ccgccattgc ttcgctctcc accacttcca tgctttttta accgcgcagc
     3721 gccccggtca gagcccgaag acccgcttcg cgcggccgca gatgatctcg tatttctcgt
     3781 cctcgggcac gccggcgaac agattttcga tgcactgccg gctttcggga tagctggttt
     3841 ccgagtgcgg gtagtccgac gaccacatga tgttcctggc ccccggcatg tggcgggtat
     3901 cgacgcccac cttgtcgtgg atgaacgaac cgtagacgtt ctggtccatg tagaagctgg
     3961 gcggctcggt cagcttgttc ttcgtccagt accgctgctt ctgccaggtc atgtccatgt
     4021 aattggcggc gaaggcgaac cagcccacac cgctttccac gctgacaaag cgcagcttcg
     4081 ggaagcgctg gaacacgccg ccgaaaatca gcagcgcgat cggttcggcc atggcgagct
     4141 tggtcatgag caggtccgac aggaagaacc gcggctcctg ccagcgcgcc atgcgggcgc
     4201 ccaggtggat gcagatcgtc atgtcaaggt cctgggcctc ggcccagaac cggtcgaact
     4261 gcggatcgtc gtactgcagc tcgccaaagg gattgccggt caacgccagg gtctgcgctc
     4321 cgccggcgat atccatctcc ttgagcgtct tggcctgggg gaaggcagga atgttgcagg
     4381 ccttgaaccc gcgcttggcc gcggcgcgca tcatggtgat cgtctcgtcg atatcccgca
     4441 tggggagata ggcgacaccg atcaggcgct tgggctcata ggcacagaag tcagcgagcc
     4501 agcggttata ggcatcgtag ctctcgatgt agagttcgtc gctggtcgtg ccgagcggtc
     4561 cgccgccgaa catcaccgcc gcatccagcc cgtcctggtc catgtccttc aggcgctcgt
     4621 ctggcaacca accgccgccg cgcatgtcgg acagccggcc ttcgatcttg aagttctcgc
     4681 ccgaccggcc cgcctgcgcg ccgatcaggt tgagcttctt ccggcgcccc tcgaagacca
     4741 cgtaatccgc atcgtcaccc tgctcgattc ggggggccag atgccgcagc gattcaggca
     4801 ggtagtcctg ccagaagtcc ggcgcggggt tcacgtggga atcggcgcag atgaccttgt
     4861 aatcgaccat ggttactctc cgaattcgat atatggaatt ttatgtcgta atacctgact
     4921 tttacatggt cgccccaccc cgtcaacccg cgtgagatct gccgtggcat ttcacgccgc
     4981 agtcgcccgc cgttgctcca ggctatcaag gaaggcccgg tatccttcgg gctcccggcg
     5041 cttcgccgga tcgtagaacg gcgaaaggac cttgagcatc atcggcagga tccggcgggt
     5101 ggtgatcctt ttgacctcgg cctcgcgggc gagagattgg gccagttcgt cggccgacat
     5161 ggcttcccgg tctgtggtgc gcaggtactg ccccgccgcc ttcacgaagc gcccgatgtg
     5221 gatgagcgcg taggcgtagc cgtagatgcg atagaaatat cgcgaaacgg gatcgagccc
     5281 gctcagggca tggaacactt ccgaacagac cgtgcggtgt tcgaactctt cggcaagatg
     5341 ccagcgccag agatcggccg ttgccggatc ggcgccttcg agcagttcgc cgtaatcctc
     5401 gaaccaggct tcgcagccgc tggcgctcat cgattcgaac ccttcgcagt aggcaaggtt
     5461 gaaccggagc gagcgctgtt cgaggaaggc cttgtagtcg gcctgcatct tgtcctcgag
     5521 cggcttgagc ccttcatagc cttggcgccg aatcgcggca ttgaacgcca ggtgctgctt
     5581 gcagtgctgc atctcctgtt tgcagaagat gtcgatctcc tcgatcagcc gggtgttggc
     5641 cggatcgatc ttgtccctgg ccgtcttcat caccttcagc agaaatggct cgacgtaagc
     5701 cggcaccagc gaggcggcgt tgcaccgctg cgcaaattcg ggcgaacgcg tccagtgcgc
     5761 gcgcacattg gagaagtcga aggtcgggaa gcgggctttc atcgtctact cctgtcccgg
     5821 cgggccggga tgccggtgcc gaatgcggcc atgtcgaacc ggaagggggc tgcaacgcga
     5881 ggcgccgcca ccgcgccgat ccgccgcgtg ccgccttgca cccagtatct tgccgtcccg
     5941 gtcggcggtc atggcttgcc attttggcga aatacaattc taataggcga attctggcag
     6001 caattgccca aatgatcaat tgcctgaggt ttcgaccgca aacctcaagg caaaaccgga
     6061 gagaatgcat ttgaccgccc ccgcccccac gttcagcccc gctcccctgg tccttgagga
     6121 ggtgctcgat cctgcctggc tcggcatggc cctgggcggg atcgaggtgc gcgacgtgcg
     6181 agtcgtcgaa acgctcggcc catcatgcac caaggtgcgc atggagctgg cgttcggccc
     6241 cggcgtcgat ccggccatgc cgacggccta ctgcctcaag ggcttcttcg gcgatcccgc
     6301 gctcggctat ctccagtccg gcacgcagct gaccgaggcc gccttttacc gcgattgcgc
     6361 accgcacctg accatgcggc tggcggaatg cgtccatgcc ggtagcgacg agcagaccgg
     6421 cgcgggggtg atcctgatga aggacctcat tgcaggcggc gcccggttcc tgacggcgct
     6481 tgaaccctac tcggtcgagc aggcgcgcag cagcgtcgac cagctggccc ggcttcatgc
     6541 cgccagctgg ggtgaggcga agctggcgcg ttggccctgg gtgcgcccgc gcgtcgagga
     6601 gctgaccaac tacaagggcg tgcccaggga gcggcttacc gagctgatgc ggggagagcg
     6661 cggcgagccc ctgccggatg agctgcgcga cggccagcgc atctacgcgg ccctgaccag
     6721 catcgccgga cgcgacgcgg gcctgcagca ctgcctcgtc catggcgatg cccatgccgg
     6781 gaacgtgttc gaaacgcccg aaggctgcgg catggtcgat tggcaattgc tgcagcgcgg
     6841 ccattggtcg ctcgacgtcg cctaccatat cggggcggtg atgacggtgg agaaccgccg
     6901 ccagcacgag gaaagcctgc tccggcatta tctcgaccgc ctcgacgccc atggcggaca
     6961 ggcgccggcc tgggatgagg cgtgggacag gtaccgggag tctctggctt acgggatgtt
     7021 cctgtggggg atcacggtgc gggtcgatcc gccgatcatc atggaattca atcgccggct
     7081 gggcaccgcc gtcgccgatc acgagagctt ccgtcgcctt ggcgtctgaa ccctccgcat
     7141 ccgtggctgg acgtgctgga aaacagctct ttgcgtcggt caacggcgcg cgtaagggcg
     7201 ggcagttcga aatggcgaaa gcttccatgt ccagagcctc acccgcagcc acccgcgtcg
     7261 tatccgtgat caactttttc gtggagcatc cgtccgatgc gttcacgctg acggaactcg
     7321 tcaaatcgct ccggctgagc cgggccacgt gccatgccct gctggcgact ctggtcgaca
     7381 agggctatct ctatcgcaag cccgacaaga gctatgttat tggcccggcg ctcgttgcgg
     7441 ccggccggat cgcgcgcgaa aactattctc cgctcgagat cgcgcgggcg gagatgcgcg
     7501 ggctcgctga cgaattcgat gcgatctgca cggcccagtt catcgaaaag gatgaaatgg
     7561 tgctgcgcga ccgcgctggc ggcatttccc acctcgggtg gctcccgccg atcggtcagc
     7621 gggtaagcat gaattcgctg tgggctgctc cttcgatgtc gttcgagagc gacgaagcga
     7681 tcgcccaatg gctcgatacg atcaagaacg aggctacgcc cgagcagcgc gaggaagtcc
     7741 gtgcggcaat ggatgcggcc cgcgaacggg gctacttcgt caccgtccgg cttgacagct
     7801 ggtccgtcca cctggcccag gccccgatcg ccgagcgccg ggagcacacc aatttcttcg
     7861 tgcgcgagct cgacgacgat cgttcgtacc agctgagctt catcctggtc ccggtcatgg
     7921 acgcgaaggg gctggctttc gtcatgaccc tgtccgggtt cctgcacgag gtcaccggca
     7981 agaagatcaa gcagatcgcg gaaaccgccg tggccgccag ccagcgcatt tcccggttcc
     8041 tcaagcatgc cgacggagtc agtccggcct agggccgggg ccggcatctc gttccacacc
     8101 caacgcgcag gagcatcgat gtcggccgaa atattcccgt tccatgccgt ggtctccgat
     8161 gaagtgctgc gcgacctcgc tgcccgcctg gcccgcacgc gctatcccga ccagcttgag
     8221 ggcgcgggct gggattacgg gacggaactc ggctatctga gccacttctg cgaatattgg
     8281 cgaaccggct acgactggcg ggcggccgaa gcccggttca acgccttccc ccagttcgtg
     8341 acggaagcgc agggtgagcg cctccacttc taccatgtcc gctcacccga gcccgatgcc
     8401 ctgccgctga tcgtcaccca
//
LOCUS       DATNBV010000579         3695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_1914264,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000579 DATNBV010000000
VERSION     DATNBV010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914264"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VN627_13680"
     CDS             complement(<1..1083)
                     /locus_tag="VN627_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXD04348.1"
                     /translation="MTPVRTTCPYCGVGCGISATLTGVRTAEVKGDVDHPANAGRLCS
                     KGTHLGETTSLEGRLLRPMIGKRNVSWDKALDLVARRFADTIARHGPDSVAFYVSGQL
                     LTEDYYVANKLMKGFIGSANIDTNSRLCMSSAVAGYIRAFGEDVVPAAYDDLEAADLI
                     VLVGSNTAWCHPIVYQRIQAARAARGTKLVVIDPRRTETCEDADLHLALKPGTDVALM
                     NGLLAHCWRTGLIDEDYLAQSVNVPDGFWEALDEGHDLWSVARTCDVPHADLQRFFDL
                     FAATPRTVTLFSQGINQSLRGTDQVNAICNVHLATGRIGKPGAAPFSITGQPNAMGGR
                     EVGGLASTLASHMDFAPENVARTGRFW"
     gene            complement(1080..1418)
                     /gene="nirD"
                     /locus_tag="VN627_13685"
     CDS             complement(1080..1418)
                     /gene="nirD"
                     /locus_tag="VN627_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HXD04349.1"
                     /translation="MTAEWLDIGSVDQLPALGARTLPVQGAREIAIFHTANGDVYALV
                     NQCPHKQGPLSQGIVHDTSVTCPLHNWRISLITGKALGEDEGCVPVIPVKIDGGRILI
                     SRKEALQGMA"
     gene            complement(1466..3496)
                     /gene="nirB"
                     /locus_tag="VN627_13690"
                     /pseudo
     CDS             complement(1466..3496)
                     /gene="nirB"
                     /locus_tag="VN627_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
     gene            3404..>3695
                     /locus_tag="VN627_13695"
     CDS             3404..>3695
                     /locus_tag="VN627_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04350.1"
                     /translation="MDDGHLHALQAEAMGGFQPQQAAADHDRVAAQLGRDQIEDSIDR
                     LRRAEPSEAFPLRVPRREAIAAAVLALALAPLVLLGPAARRDATGPRVDQIAR"
BASE COUNT          617 a         1282 c         1170 g          626 t
ORIGIN      
        1 ccagaagcgg ccggtgcgcg ccacattctc cggcgcgaag tccatgtggg aggcaagggt
       61 cgacgcaagc cccccgactt cgcggccgcc catggcattg ggttgaccgg tgatcgaaaa
      121 gggcgccgcg cccggcttgc cgatgcggcc ggtggcgagg tgcacgttgc agatggcatt
      181 gacctgatcg gtgccgcgca gcgactggtt gatgccctgg ctgaataggg tgacggtgcg
      241 cggagtcgcc gcgaacaggt cgaaaaagcg ctgcagatcg gcgtgcggaa catcacaggt
      301 ccgggcgacc gaccaaagat catggccctc gtccagcgcc tcccagaagc catcgggaac
      361 attcacgctc tgggcgagat agtcctcgtc gatgaggccg gtgcgccagc aatgggccag
      421 cagtccgttc atcagcgcga cgtcggttcc aggcttcagc gccagatgca gatcggcatc
      481 ctcgcaggtt tcggtacgcc ggggatcgat cacgaccagc ttggtgcccc gggcggcccg
      541 cgccgcctgg atgcgctgat aaacgatggg atggcaccag gcggtgttcg agcccaccag
      601 cacgatgagg tcggcagcct ccagatcgtc ataggcggcg ggaacgacgt cttcgccaaa
      661 ggcgcggata tagccggcga ccgcgctcga catacaaagg cgcgaattgg tgtcgatatt
      721 ggccgatccg atgaagccct tcatcagctt gttggcgaca taataatctt cggtgagcag
      781 ctgtcccgag acatagaacg ccacgctgtc cggcccgtgc cgcgcaatgg tgtcggcaaa
      841 gcggcgggca acgaggtcca gcgctttgtc ccagcttaca ttgcgcttgc cgatcatcgg
      901 gcgcagcagg cgtccttcga ggctggtggt ttcccccaga tgcgtgccct tggagcagag
      961 gcgcccggca ttggcggggt ggtccacgtc gcccttgacc tccgccgtgc gcacacccgt
     1021 cagggtcgcg gaaatgccgc aaccgacgcc gcaatagggg caggtagtgc gaactggcgt
     1081 catgccatgc cctgcaaggc ttccttgcgg ctgatcagga tacggccgcc gtcgatcttg
     1141 accgggatca ccggcacgca gccttcatcc tcgcccagcg ccttgccggt gatgagcgag
     1201 atgcgccagt tatgcagcgg gcaggtcacg ctggtgtcgt ggacgatccc ctggctcagc
     1261 ggcccctgct tgtgcgggca ttggttgacg agcgcgtaga catcgccatt tgcggtatgg
     1321 aaaatcgcga tttcgcgcgc accctgcacc ggcagcgtcc gcgcgccaag ggcggggagc
     1381 tggtccaccg atccgatgtc gagccactct gcggtcatat gtttgtccgt tgcattttca
     1441 ggttgaacca agcgtagcgc tacattcacc ggcccgcccc cacaacagca aagggcctga
     1501 tttcggcgat atgctggtgg aggtgggctt cggcgccggc ggcgcgctcg gcccaggggt
     1561 cgtgctggag gaagctctgg gcgtggagga agcggctgcg cagcgcctcg cggccgacct
     1621 cgtccataac gatgcgctgc ttcacatagt cgagcccgac ccgctccacc cagggcgcgg
     1681 tgcggtccag atagcgcgcc tcctcgcggt agagctggat gaacgccgcg caatagtcga
     1741 gcgcctcctg ctcggtcgcg accttgcaca gcaggtccgt gccgcgcagg tggatgccgc
     1801 cattgccgcc gatatgcagc tcatagcccg agtccacgca gatcacgcca aagtccttga
     1861 tcgtggcctc ggcgcagttg cgcgggcagc ccgacaccgc gatcttgaac ttgtgcggca
     1921 tccagctgcc ccaggtcatc tgctcgagct ggatgccaag cccggacgag tcctgcgtgc
     1981 cgaaccggca ccattcggac cccacgcagg tcttcaccgt gcggagcgcc ttgccatagg
     2041 catggcccga gaccatgccg gccgcattga ggtcggccca gatcgcgggc agatcctcct
     2101 tcttgatgcc gaacaggtcg agccgctggc cgcccgtcac cttgaccatg cgcgcgccat
     2161 atttgtcggc ggcatccgcg atcgcgcgca gctcattggg cgtcgtcacc ccgccccaca
     2221 tgcgcggcac caccgaatag gtgccgtcct tctggatatt ggcgtgcatc cgctcgttga
     2281 cgaagcggct ctgcgggtcc tcgacatgct cgcccggcca ggcgcacagc agataatagt
     2341 tgagcgcggg ccggcacgag gcgcagccat cgggcgtcga ccagctttgc gcctgcatca
     2401 cctgcgggat ttctttcagc cccttgtcgc ggatcgcggc gcgcacttcc tcatgggtga
     2461 agctggtgca ggcgcacatc ggcttgaccg cgcgttcgcc cgcataatcg tcgcccaggc
     2521 tcagcgcgag caggttctcg accagcccgg tgcacgagcc gcagctggcc gaggccttgc
     2581 aaccgctgcg caccgcatcg agcgtgcccg cgccaccgga aatgcaggcc atgacctggc
     2641 ccttgctgac gccgttgcag ccgcaaatct ccgcatctgc agagagcgcc gcaacggccg
     2701 ccctagggtc cgccagcgca cccccggacg cgaacgcctg cccgaagatc agcgcctctc
     2761 ttatggaagc gatgtcttcc tgtttcttca gcaggtcgaa ataccagttg ccgtccgccg
     2821 tgtcgccata gagcaccgcg ccgatcacgc ggtcctcttt cacgatcacc cgcttgtaga
     2881 tgccccgcgt cgcgtcgcgc agcacaatgt cctcgcagcc atccccgccg gaaaagtcgc
     2941 ccgccgagaa cacgtcgatc cccgatacct tcagcttggt cgaggtcacc gagccgcggt
     3001 agggattctc ggcgcccacc agcccgtccg ccaacgaccg gcacatctcc cagagcggcg
     3061 ccaccaaacc atagacattg ccgtcatgct cgacgcactc gcccacggcg aggacatcgg
     3121 cgtcggaggt gaccatgtga tcgtccacat ggatgccgcg tcccacggcc agacccgcag
     3181 ctttcgccag gatcgtgctg ggacggatgc cgaccgccat gacgacgatg gaacagggaa
     3241 tctccgtccc atccttcagc cgcacaccct cgaccttgcc gtcgccaaag acttcggcgg
     3301 tatcggcttg cgtcaggatc gtctggccgc gcttttccag ctcattcttg agcagccagc
     3361 ctgccgcctc gtcgagctgc cgctccatca gcgtcggcat caggtggatg acggtcacct
     3421 tcatgccctg caggctgagg ccatgggcgg cttccagccc caacaggccg ccgccgatca
     3481 cgaccgcgtc gccgcccagc tcggccgcga ccagatcgag gactcgatcg accggctgcg
     3541 ccgggccgag ccgtccgagg ctttcccgct gcgggtaccg cggcgcgagg ccatcgccgc
     3601 cgccgtgctg gcgctggcgc tcgccccgct cgtcctgctc gggccggccg cccggcgcga
     3661 cgcgaccggt ccgcgcgtcg accagatcgc ccgcg
//
LOCUS       DATNBV010000580         3700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3158130,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000580 DATNBV010000000
VERSION     DATNBV010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3158130"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..756
                     /locus_tag="VN627_13700"
     CDS             49..756
                     /locus_tag="VN627_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXD04351.1"
                     /translation="MELTVASYNIHKAVGLDRRRDPERIMAVLREIDADVIALQEADR
                     RFGDRVSVLPRQVLEDHGHWQAVALGQRPDSIGWHGNAVLVRRGIEVADADIVPLPTL
                     EPRGAVRVDLVKHGQPVRVVGMHLDLSGLRRRQQVRTVLAHVEICGEACPTVLMGDFN
                     EWSRTGGCLHEFHHDDWRVLAPGRSFPSRRPVAMLDRIVASADWDCGHCAVHHSALAA
                     RASDHLPVHARLTLLKK"
     gene            908..1246
                     /locus_tag="VN627_13705"
     CDS             908..1246
                     /locus_tag="VN627_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXD04352.1"
                     /translation="MKFIIAIIKPFKLDEVREALGAVGVAGMTVSEVKGFGRQKGQTE
                     IYRGAEYSTNMLPKVKIEIAAADDLAPQIVETIQQVASTEAIGDGKIFVLDLAEAVRI
                     RTGEAGDTAL"
     gene            1263..2746
                     /locus_tag="VN627_13710"
                     /pseudo
     CDS             1263..2746
                     /locus_tag="VN627_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993554.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
     gene            complement(3143..3574)
                     /locus_tag="VN627_13715"
     CDS             complement(3143..3574)
                     /locus_tag="VN627_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HXD04353.1"
                     /translation="MFKTIVDGVYASPQIGLPEVELAKAMGVTLIINNRPELESPDQT
                     PGAEIEAAARNAGIAYVAIPVTHAGFSEPQVAAMARALEGAAGPVLAYCRSGTRSTLL
                     WALAEASTGADPDDLAAAAAQAGYDVSPIRTLLDMLAARAG"
BASE COUNT          586 a         1189 c         1239 g          686 t
ORIGIN      
        1 caattgcatc ggggttgcaa cgcaactgca aggcttcaca atgccgcgat ggaactgacg
       61 gtcgcctcct acaacatcca caaggcggtg gggctcgacc gccgccgaga tcccgagcgc
      121 atcatggccg tgctgcgcga aatcgatgcc gacgtgatcg ccctgcagga ggccgaccgc
      181 cggttcggcg accgggtcag cgtgctgccg cggcaagtgc tggaggacca cggacactgg
      241 caggccgtgg cgctcggcca gcggcctgat tcgatcggct ggcatggcaa tgccgtgctg
      301 gtgcggcggg ggatcgaagt ggccgacgcc gatatcgtgc cgctgcccac gctggagccg
      361 cgcggggcag tgcgggtgga cctggtcaag cacggccagc cagtacgggt cgtgggcatg
      421 catctcgatc tgtcgggcct gcgccggcgc cagcaggtgc gcacggtgct ggcccatgtc
      481 gagatctgcg gcgaggcctg ccctaccgtg ctgatgggcg acttcaacga atggtcgcgc
      541 accggcgggt gcctgcacga gttccatcac gatgattggc gggtgctggc gccgggtcgc
      601 agctttccca gccggcggcc ggtggccatg ctcgaccgga tcgtggcctc cgcggattgg
      661 gactgcggcc attgcgccgt tcaccacagc gctctggcgg cgcgtgcctc ggatcacttg
      721 ccggtccatg cccggctgac gctgcttaaa aaataggcag tttgcacagg attcaggcgc
      781 tgtccttact ccgggattgg tgggaccaaa gtgactcaat cggaatccta acgattccct
      841 aaactggcac gctccttgcg aagcggatgc cgccggcgac gggtccggca ggctccaggg
      901 ggcaaggatg aaattcatca tcgccatcat caagccgttc aagctggacg aggttcgcga
      961 agccctcggc gccgtaggcg tcgcgggcat gaccgtttcc gaggtcaagg gttttggacg
     1021 gcaaaaaggc cagacggaaa tttaccgcgg cgccgaatat tccaccaaca tgctgcccaa
     1081 ggtgaaaatc gagatcgcag cggccgacga cctcgcgccg cagatcgtcg agacgatcca
     1141 gcaggttgcc agcaccgaag ccatcggtga cggcaagatc ttcgtgctcg acctggccga
     1201 agccgtgcgc atccgcaccg gcgaggccgg cgacaccgct ctgtaacggc aggagggaaa
     1261 ccatgatgcg taaactcgct tatggtgctg gcgcgatcgg agcctcgctc ttcgccgcca
     1321 ccaccgcttt cgcgcaggag gcgacggaag ccgccactgc ggctgcgtcg accgcagccg
     1381 cggctgcgac caccgcggcg gccaccgcca ccagcaccct gatcgccgcg ccgaccgata
     1441 cggcggcgat ggtcaacaag ggcgacacca gctggatgct gatctcctcg gcactcgtgc
     1501 tgatgatgtc gatcccgggc ctcgcgctgt tctacggcgg cctggtccgc acaaagaaca
     1561 tgctgagcgt ctgcatgcag gtgttcatga tcgtggccat tgccggtctg gtctgggcct
     1621 gctggggtta ctcgctggcc ttcactggcg gctcgacccc gtttgtcggc ggcctctcca
     1681 aggccttcct gatgggcgtg accgccagct cgaacgctgc gacgttctcc aacaacgtct
     1741 acatccccga gctggtctac atggtcttcc agatgacctt cgcgatgatc accccggcgc
     1801 tgatcgtcgg cgctttcgcg gaacgcgtga agttcactcc gttgatgatc ttcatggtcc
     1861 tgtggatgac cgtggtctac ttcccgatcg cgcacatggt ctggtactgg gccggtccgg
     1921 acttcctctc ggccaatccg actgacgcgg gctatctgtt ccaatgggcg cgctcgattt
     1981 cgcgggcggc accgtggtcc acatcaacgc gggtatcgcc ggcctcgtcg gctgcctcat
     2041 gatcggcaag cgcctcggct tcggcaagga agccactccg ccgcactcgc tgaccatgac
     2101 catgatcggc gcctcgctgc tgtgggtggg ctggttcggc ttcaacgccg gctccaacct
     2161 tgaagccaac ggcgtgaccg gcgtggcctt cgtcaacacc atgctcgcca cctgcgcggc
     2221 cgccgtctcc tggtcggtga tcgagcagat ccaccacggc aagccctcga tgctgggcgg
     2281 cgccacgggt gcggtggccg gcctggtcgc catcactccg gcctcgggct tcggcgcacc
     2341 gatgacctcg atcgtgctcg gcctgctggt ttcgccggtc tgctacttct tcgtcagcgt
     2401 ggtgaagaac aagctgaagt acgacgacac cctcgacgtg ttcggcgtgc actgcatcgg
     2461 cggcatcttc ggcgccatcg ccaccggcat cgttgccgac ccggcgctcg gcggccaggg
     2521 cttcttcgac tacacgaagt tccctgccgt tcccggcacc tatgacatgg ccgcccaggt
     2581 ggtcatccag atcaaggccg tgctgctgac gctggtctgg tccggcgtgg gttcggcggt
     2641 gctctacttc atcgtcgaca aggtgttcgg tctgcgtccg acggctgatg ccgagcgcga
     2701 aggcctcgac atctccgagc acggtgaacg cgcctacaat atgtgatctg accagtttgg
     2761 cggggccgga gactttcctc tcctccaccc ttcggccccg ccatccgacg ttcctcctgc
     2821 gaacgcaaac ttagcccgga gccagccgct ccgggctttt tttgtgtgtt gggggaaagg
     2881 ggaagagtgg ggttcacgca gaggcgcaga gaagaaaggg gagacgcgga ggggttgtga
     2941 ctgcggcgaa gcctggcttg acccaatgga ttcggggatg gcttggtggg agtggtcgcg
     3001 tcctcaccgc gccctttgct tccgctgcgg tccggcgggt agtctggtgg gagcgcggtt
     3061 gcgctattgc ccctccacgg cccgcttgga gggcggtccc ccctccccat ttatggttcc
     3121 caaatacggg gaggatcttg gctcagcccg cgcgggctgc gagcatgtcc agcagggtgc
     3181 ggatcgggct gacgtcgtaa ccggcttgcg ctgcggcagc ggcgagatcg tcggggtcag
     3241 cgcccgtgct ggcttcggcg agggcccaca gcagggtgga gcgggtgccc gagcggcaat
     3301 aggcgaggac ggggccggcg gcgccttcca gcgcccgggc catggcggcg acctgaggtt
     3361 cgctgaagcc ggcgtgagtg acgggaatcg cgacgtacgc gattcccgcg ttccgggcgg
     3421 ccgcctcaat ttcggcgcca ggggtctggt cgggcgattc cagttccggc cggttgttga
     3481 tgataagggt gacgcccatg gccttggcga gttcgacctc ggggagcccg atctgggggc
     3541 tggcgtagac gccatcgaca atggtcttga acatgtccgt cagctcctgg cggtgcggga
     3601 ctcttcctgc ttgccgcgca ggcgggcccg acggttgcgg ccgatgatgt ttaggctttc
     3661 caccgcagcg gaaaaagcca tggcggcata gacgtaaccc
//
LOCUS       DATNBV010000581         2048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4453980,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000581 DATNBV010000000
VERSION     DATNBV010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4453980"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(464..1624)
                     /locus_tag="VN627_13720"
     CDS             complement(464..1624)
                     /locus_tag="VN627_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXD04354.1"
                     /translation="MEGKGSDRVDVASRTLYVAIELSRSGWLAGVLGHGGQRASRHKL
                     KAGDVDGLLALIARTRGTATRVCSCYEAGYEGFWLHRRLVAAGIDSRIVDPSSLQVDR
                     RARRKKTDAIDVEMILRALVAWDRGERGLCAMVRVPDVAAEDARRTSRERERLVKEQT
                     GHLNRIRGLLTTMGIYDFNPRRTNRLAVLATLMTGDGRELPERLTVEIRREIDRLQLV
                     ERQIAELERERNPPAPRGRPRKDAIPRKPDPASPPGRIAHLLQLRGVGPEIAGVLQSE
                     VFYRRFGNRRELTAYCGLDPTPFASGATHREQGISKAGNRRARHAAVELAWLWLQWQP
                     DTALARWFHARVGLATGRLKRTMIVALARKLLIALWRFLEAGVVPDGAALKA"
     gene            complement(1750..>2048)
                     /locus_tag="VN627_13725"
     CDS             complement(1750..>2048)
                     /locus_tag="VN627_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04355.1"
                     /translation="VISNQGGTTFKDVLFAAARAPGDAFGTFAVGDVAQAAAAHGLVL
                     SSLALQLPLKKLTEPGRGALLRRIAGLDGLRYQFVNQRARHHVLVRQAVERGLV"
BASE COUNT          310 a          677 c          713 g          348 t
ORIGIN      
        1 ggcctccgtc tgctacgttg ttctgtgttt gttcacgttc tttgacattg tcaatcatgt
       61 cagcggatcc caccgggacc gcacccgccg tccaaccgcc cgagcgctgc ggtggcggcg
      121 gttctctcca tgccatcgcc cgcatcgctc cgaaccacaa gatctgggaa cgctgcctca
      181 tatggggtgg gatttcgtca agtcggttgt ttctccttgg tgaagggggt tgggattttg
      241 aatccaaggc agggcctgct ttttgatgcg gagccgtgct cgacatcacc tcatatccgg
      301 ttcggccggt tgcggccgtg accccaatcg cgcattctgg cgtatgcagg cccaaggtcg
      361 tcgggatcct tctgtaaagc ggtggtctac ccaggcaggg ttgcgatcac acgacggcct
      421 tggatgtggc ggcgagctgg ccggtggtgc ggctggctcg ccgttacgct ttgagggcgg
      481 cgccgtcggg gacgacgccg gcttcgagga agcgccagag ggcgatgagg agctttcggg
      541 cgagcgccac gatcatggtg cgcttgaggc gcccggtggc gaggccgacg cgggcgtgga
      601 accagcgggc gagggcggtg tcgggctgcc actggagcca gagccaggcg agctcgacgg
      661 cggcgtggcg ggctctgcgg ttgccggcct tgctgatgcc ctgctcgcga tgggtggcgc
      721 cgctggcgaa gggggtcggg tcgaggccgc aataggcggt gagctcgcgg cggttgccga
      781 agcgccggta gaagacctcg ctttgcagga caccggcgat ctcgggcccg acaccacgca
      841 gttgtaggag atgggcgatg cggcccggtg ggcttgctgg atcgggcttg cgtgggatcg
      901 catccttgcg gggtcggccg cgcggcgccg gcggattgcg ctcacgctcg agctcggcga
      961 tctggcgctc gaccagctga aggcggtcga tctcgcggcg gatctcgacg gtcaggcgct
     1021 cgggcaactc gcggccgtcg ccggtcatca aggttgcgag caccgcaaga cggttggtgc
     1081 ggcgcgggtt gaagtcgtag atgcccatgg tcgtgagcaa gccgcgaatg cggttgaggt
     1141 ggccggtctg ctccttgacc aggcgttcgc gctcgcggct ggtgcgccgg gcatcctcgg
     1201 ccgcgacatc gggcacgcgg accatggcgc acagcccgcg ctcgccgcgg tcccaggcga
     1261 cgagggcgcg caggatcatc tcgacgtcga tggcgtcggt cttcttacgc cgggcgcggc
     1321 ggtcgacctg cagcgagctc gggtcgacga tccggctgtc gatgccggcg gcaaccaggc
     1381 gccggtgcag ccagaagcct tcgtagccgg cctcgtagca cgagcacacc cgtgtcgccg
     1441 tcccacgcgt gcgagcgatc agcgccagca gcccatcgac atcgccggcc ttcagcttgt
     1501 gccgcgaggc tcgttgccca ccgtgcccga gaacccctgc cagccaaccc gagcggctca
     1561 gctcaatggc aacatacagc gtcctcgacg ccacatcgac ccgatcagaa cctttccctt
     1621 ccatggcaca cccctcaaga ttgagacgcg tccatgatca ccatcggacg cccccgcgcc
     1681 ccatgggatc tgtaaaggac ctgtatttac aggccgaccc accagatcta gaggtcatcg
     1741 gctagcaacc tagaccagcc cccgctcgac cgcctgccgc accagcacgt ggtgccgcgc
     1801 ccgctgattg acgaactggt agcgcagccc gtcgaggccg gcgatgcgcc gcaacagcgc
     1861 cccgcggccc ggctcggtca gcttcttcag cgggagctgc agggcgaggg acgagagcac
     1921 cagcccgtgc gccgccgcag cttgcgccac atcgccgacc gcgaaggtgc cgaaggcgtc
     1981 gcccggagcc cgtgccgccg cgaacagcac gtccttgaac gtcgtgccgc cctggttgct
     2041 gatgaccg
//
LOCUS       DATNBV010000582         2238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3162874,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000582 DATNBV010000000
VERSION     DATNBV010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3162874"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VN627_13730"
     CDS             complement(<1..1374)
                     /locus_tag="VN627_13730"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HXD04356.1"
                     /translation="MVTFNRTDLDYILDQIRLAEDGQVPSNTQLAFGLREVAGTNNNL
                     LPGQSEFGSVDQPLPRVGAPVFRTVQQMSLIDAFTNPLGYLTQGVDAAGNIVMGTTGQ
                     VANEIDEFVTGALRNNLLGLPLDLAAINIARGRDTGVPPLNVLRNQLFTQTGDSNLKP
                     YANWAEFGQFLKHAASLINFVAAYGTHASVLGATTLEEKRAAALELVVAGLDEANIGT
                     DAWDFMHSLGAYANDVDNAIAVHGEWSTGSVTGLDAVDLWIGGLAEKQILFGSLLGST
                     FQFIFETQLEALQDGDRLYYLPRIEGIHFGDEIEGNSFAALIAANTSARHLPGDVFLT
                     PEYVIEAGTITDDPATWLRNPETGALLVERLPDGTIRFIGDDNFLGNTIVLGGTEGDD
                     RLVAGHADDDTVWGDGGNDFIEGGAGDDLITDSGGDDIIHGDDGNDTIHGGIGDDAIF
                     GGDGNDLI"
     gene            1959..>2238
                     /locus_tag="VN627_13735"
     CDS             1959..>2238
                     /locus_tag="VN627_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04357.1"
                     /translation="MTAAAGDHETAARKLEIAVRSAGVGPRFAAALSAWMIRQARSVS
                     GLDYSLIDYRGMGLAASQLSFEWWLEPQRTVVALTELWWRNLALIANHA"
BASE COUNT          364 a          698 c          764 g          412 t
ORIGIN      
        1 gatgaggtcg ttgccgtcgc cgccgaagat ggcgtcgtcg ccgatgccgc cgtggatggt
       61 gtcgttgccg tcgtcgccgt ggatgatgtc gtcgccgccg ctgtcggtga tgaggtcgtc
      121 gccggcgccg ccttcgatga agtcgttgcc gccgtcgccc cacaccgtat cgtcgtcggc
      181 atggcccgcg accaggcggt cgtcgccctc ggtgccgccc agcacgatgg tgttgccgag
      241 gaagttgtcg tcgccgatga agcggatggt gccgtcgggc agccgctcca ccagcagcgc
      301 cccggtctcg gggttgcgca gccaggtggc gggatcgtcg gtgatggtgc cggcctcgat
      361 cacgtactcc ggggtcagga acacgtcgcc gggcaggtgg cgggcgctgg tgttggccgc
      421 gatcagcgcg gcgaacgagt tgccttcgat ctcgtcgccg aagtggatgc cctcgatgcg
      481 gggcaggtag tagagacggt cgccgtcctg cagcgcttcg agctgggtct cgaagatgaa
      541 ctggaaggtg gagccgagca ggcttccgaa caggatctgc ttctcggcca ggccgccgat
      601 ccacaggtcg acggcatcga ggccggtgac cgagccggtg ctccattcgc cgtggacggc
      661 aatggcattg tccacgtcgt tggcgtaggc cccgaggctg tgcatgaagt cccacgcgtc
      721 ggtgccgatg ttggcctcat cgaggcccgc caccaccagc tcgagcgccg ccgcgcgctt
      781 ctcctccagg gtggtcgcac cgaggaccga ggcgtgcgtg ccgtaggcgg ccacgaagtt
      841 gatcagcgac gcggcgtgct tgaggaactg cccgaactcc gcccagttgg cgtagggctt
      901 caggttcgag tcgccggtct gggtgaagag ctggttgcgc agcacattga gcgggggcac
      961 gccggtatcg cggccgcgcg cgatgttgat ggcggcaagg tcgagcggca ggcccagcag
     1021 gttgttgcgc aaggcgccgg tgacgaactc gtcgatctcg ttggcgacct ggccggtggt
     1081 gcccatgacg atgttgccgg cggcatcgac gccttgcgtc aggtagccga gcgggttggt
     1141 gaaggcgtcg atcagcgaca tctgctggac ggtccggaac accggcgccc cgacgcgcgg
     1201 caacggctga tccaccgagc cgaactccga ctgacccggc agcaggttgt tgttggtccc
     1261 cgcaacctcc cgcagcccga acgcaagctg cgtgttcgac ggcacctggc catcctcagc
     1321 cagacggatc tgatcaagaa tgtagtccag atccgtcctg ttgaatgtga ccatcgcctc
     1381 tacctcaccg aattggctct aaagcgcccg acctattatt gcgcacaatg cggctcctta
     1441 gtgcccatca acaagagagg aatgccctag ggccgcagac cggcgctgct gtccagacgg
     1501 acagaggagt gtggcctttg gacagatgca ttgccggctg ccattgccgg ctgccattgc
     1561 cggcaaagcg catggggcga tgctgatcgg attgggagcc ggtccgacaa cccgcccctg
     1621 aggcgtcgag atatcggacg ccgagtaggg tcctgaatga accctccctc tgcctccaaa
     1681 gctcgcgccc agccgccggc aaccagcgtt tcccggttgg cgaatcgggt cttgccccga
     1741 tatcgccgcg cagtcccgct cgatgagact ctgatccgga gagagggttg ccccctaaag
     1801 gtgaggcttg ggaacccatg gtgccactgc tgtgggttca gcctgcgcca aggcaacggg
     1861 ctgcgcggct catacccatt tcggctggat cagtttgggc aatgagtcag gcacgaaagt
     1921 cgcaagccgt tctgtcggcc aactcttagg tcacgagtat gacggcggcc gcaggagatc
     1981 atgagaccgc cgctcgcaag cttgaaatcg ccgtccgttc ggccggtgta gggccacgct
     2041 ttgcggccgc gctctcggct tggatgatcc gccaggctcg aagtgtgagc gggcttgact
     2101 attcccttat cgattatcga ggcatgggct tggccgcgag ccaactcagc ttcgagtggt
     2161 ggcttgagcc gcagcgcacg gtcgtggcgc tgacagaact ctggtggcgc aatcttgccc
     2221 tgatcgcgaa ccatgccg
//
LOCUS       DATNBV010000583         3648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4455718,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000583 DATNBV010000000
VERSION     DATNBV010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4455718"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1369)
                     /locus_tag="VN627_13740"
     CDS             complement(191..1369)
                     /locus_tag="VN627_13740"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXD04358.1"
                     /translation="MDAVIVSTARTPIGKSYRGSLNGTPGATLGGHAIAAAVGRSGFE
                     PGEIDDVLMGCAAQEATTSSNVARQALLRAGLPVTVPGTTIDRKCSSGLTTICFAAQR
                     IRSDGDAVFVAGGLEQCSMVQNRHRNRYRNKDPWVAEHYPAIYWGMIDTAEVVARRYG
                     IDRERQDSYSLLSQQRTAAAQAAGRFDAEIVPITTFRDEVDKAGDITGTAEVTLAVDE
                     CNRADTTLEGLARLRTVNEGGTITPGNASQISDGASACVITSSRLAERRGLEPLGIYR
                     GFEVVGCEPDEMGIGPVFAIPKLLKKHGLTVDDIDLWELNEAFAVQTIYCQERLGIPL
                     DRMNVNGGAIAVGHPWGMSGSRMTGHALIEGKRRGARLVVVTMCVGGGLGAAALFEVA
                     "
     gene            complement(1386..1901)
                     /locus_tag="VN627_13745"
     CDS             complement(1386..1901)
                     /locus_tag="VN627_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HXD04359.1"
                     /translation="MSAYLIETLPYSMPGIDARFMTPETVNHLPEDPRAWVPRPGGGE
                     YLPCMFDTCSGTMANVYRYTKPGGIGRHLHSSPVYAYTLEGSWGYKEHDWTAKKGTFV
                     FEAPGEVHTLEIYEAPMTAFFVWMGGMQAMDENDNVIAATNVHTLIAACDKHYREVGL
                     GADYIRQFVRG"
     gene            complement(1927..2658)
                     /locus_tag="VN627_13750"
     CDS             complement(1927..2658)
                     /locus_tag="VN627_13750"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXD04360.1"
                     /translation="MHDFSGKTVIVTGGAGGIGGAAAVAFARAGAEVIATGWGEAELA
                     ARRADPDFAGIRLVELDVTNDAAVEALAAQTPRLDVLVNCAGVSIRGPGTITVEAFQK
                     NYDVNMLGTLRMCNAFLPQLEASGAGAIINTASINSFKGTATASGYAASKGAVALATK
                     SMAIAWAPQNIRVNAVAPGWIDTQMSKDAASSPEFYERIIKRTPMGRWGVPEHLAGPI
                     LFLASPDAAWVTGVILPVDGGYLAV"
     gene            2939..>3648
                     /locus_tag="VN627_13755"
     CDS             2939..>3648
                     /locus_tag="VN627_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXD04361.1"
                     /translation="MNGKQAQGPERFRIGRPDLIPTARYHDPAFMQLEEERFWSRVWQ
                     MACRLEEIPEPDDFVEYRILSRSVVLIRQKDGAVKAFENACRHRGLKLCEDRGTARGG
                     ISCPFHAWHYNTDGTCRAVFQPALFDPAVLRKEDIDLVPVRVETWGGCAFITFDDHAP
                     PLRECLGPFAPMEEMRQVEDLKVALWIAIRLPANWKMAAEAFMESYHVLRSHPQLVSM
                     FGLTIEGFNEAAARGITSS"
BASE COUNT          626 a         1179 c         1191 g          652 t
ORIGIN      
        1 aggacaacgc ggacctcctc gtcctgggcg atcttcaccc agatgtcctc aagctcggta
       61 tgaacctcga gggaagcggc gttgcgcact tcggggcgat tcagagtcac cgtggcgaca
      121 tggtcaacga tatcgaccag aatgtgtttg tagcctgtgt agtccatatt cctccccgtc
      181 cgttctgatt tcacgccact tcgaatagtg ccgcagcccc gagaccgccg cccacgcaca
      241 tggtcaccac caccagcctg gcgccgcggc gcttgccctc gatcagggcg tgcccggtca
      301 tgcgggatcc gctcatgccc caggggtggc cgacggcgat ggcgccgccg ttcacattca
      361 tccgatccag cggaattccc agccgttcct ggcaatagat cgtctggacg gcgaaggcct
      421 cgttcagctc ccacaggtcg atgtcgtcca cggtgaggcc atgcttcttc agcagcttcg
      481 ggatggcgaa gaccgggccg atgcccatct cgtcgggctc gcagccaacc acttcgaacc
      541 cgcggtagat gcccagcggt tcgaggccgc gccgttcggc gagacgcgaa ctggtgatca
      601 cgcaggccga ggcgccgtcg gagatctggc tggcattgcc tggagtgatc gtgccgccct
      661 cattgaccgt ccgcagccgg gcaagacctt ccagcgtggt gtcggcccga ttgcactcat
      721 cgacggcaag ggtgacctcg gcggtgccgg tgatatcgcc cgccttgtcg acctcgtccc
      781 ggaacgtggt gatcggcacg atctcggcat cgaaccggcc ggcggcctgg gccgcggccg
      841 tgcgctgctg gctgagcaac gaatagctgt cctgccgttc gcgatcgata ccatagcgcc
      901 gggccacgac ttcggccgta tcgatcatcc cccagtagat cgccgggtag tgctccgcca
      961 cccacgggtc cttgttgcgg tagcgattgc ggtgtcggtt ctggaccatg gagcactgct
     1021 cgagcccgcc ggctacgaat accgcatcgc catcggagcg gatgcgctgg gcggcgaaac
     1081 agatggttgt gagccccgac gaacacttgc ggtcgatcgt cgtgcccgga accgttaccg
     1141 gcaaaccggc acgaagcagg gcctgtcgcg cgacattgct cgatgtcgtc gcttcctggg
     1201 cagcacagcc catcaggacg tcgtcgatct caccgggttc gaatccgctg cggccgaccg
     1261 ccgcggcaat ggcgtggccg cccagcgtgg ctcctggcgt gccgttcagc gaaccccgat
     1321 aggatttgcc tatcggggtt cgcgctgtcg aaacaatgac ggcgtccatt gcggaactgg
     1381 caggctcagc cgcgcacgaa ctgacggata tagtccgccc cgaggcccac ctcgcgatag
     1441 tgcttgtcac aggcagcgat cagggtgtgc acgttggttg cggcgatcac gttgtcgttc
     1501 tcgtccatgg cctgcatgcc gcccatccat acgaagaacg cggtcatcgg cgcttcgtag
     1561 atttccaggg tgtgcacctc gcccggcgct tcgaacacaa aggtgccctt cttggcagtc
     1621 cagtcgtgct ccttgtagcc ccagctgcct tccagcgtat aggcatagac cggcgaagag
     1681 tggaggtgac gaccaatgcc acccggcttg gtgtagcggt agacgttggc catggtgccc
     1741 gagcaggtat cgaacatgca cggcaggtat tcgccgccgc ccggccgcgg gacccaggcg
     1801 cgcggatctt cgggcaggtg gttgaccgtt tcgggcgtca tgaagcgggc gtcaatgccc
     1861 ggcatcgaat aaggcagggt ttcgatcaga taggcagaca tgcaagatac tccgtttggc
     1921 gccgggtcag acggcgagat agccgccgtc gaccgggagg atcactccgg tgacccaggc
     1981 ggcgtcgggc gaggcgagga acaggatggg gccggcaaga tgctcgggca cgccccagcg
     2041 gcccatgggc gtgcgcttga tgatgcgctc atagaattcc ggggaactgg ccgcatcctt
     2101 tgacatctgc gtgtcgatcc agcccggcgc gaccgcattg acgcggatgt tctggggtgc
     2161 ccaggcaata gccatcgatt tggtcgccag cgccacggcg cccttgctcg cggcatagcc
     2221 ggacgcggtg gccgtgcctt tgaagctgtt gatcgaggcg gtattgatga tggcgcccgc
     2281 gccgctggcc tcgagctgcg gcaggaaggc gttgcacatg cgcagggtgc cgagcatgtt
     2341 gacgtcgtag ttcttctgga aggcttccac ggtgattgtg ccgggcccgc ggatcgagac
     2401 gccggcgcaa ttcaccagaa cgtcaagccg cggggtctgc gcggccaggg cctcgaccgc
     2461 ggcatcgttg gtcacgtcga gctcgaccag acggatgccg gcaaagtcgg gatcggcgcg
     2521 ccgcgcggcc agttcggcct cgccccagcc ggtggcgatc acctcggccc cggcccgcgc
     2581 gaaggccacg gcagcagcgc cgccgatgcc gccggcacca ccggtcacga tcactgtctt
     2641 gccggaaaaa tcatgcatcg cgtgcgttca cttccacttg gttccactgc agccgaggcc
     2701 ggtcccgcgg acatcggtat atgccacaag ctgccgcgct gcgggatatc ccttgagggc
     2761 aggactgtca cgtcaggggg taatcggaca tccatgtgat cgatggccgg accggcccat
     2821 gcggtcgagc gatcggccac gccttgacaa tttgacgctt gatcaacagg ctgacgagtg
     2881 cagtctgctg ccacgcgccg gatagacaat cgcaagactg gcgtcgtgga gggacctgat
     2941 gaacggcaag caagcgcagg gcccggaacg cttccggatc ggacgccccg atctaattcc
     3001 cacggcgcgc taccacgatc ccgcattcat gcagctcgag gaggagcgct tctggtcgcg
     3061 ggtgtggcaa atggcctgcc gcctggaaga gatccccgag ccggacgact tcgtcgaata
     3121 cagaatcctg tcccgctcgg tggtgcttat ccgccagaag gatggcgcgg tgaaggcatt
     3181 cgagaatgcc tgccgccatc gcgggctcaa gctctgcgag gatcggggca ccgcgcgggg
     3241 cggcatttcg tgcccgtttc acgcctggca ctacaacacc gacgggacct gccgggccgt
     3301 attccagccg gcgctgtttg accctgccgt gctgcgcaag gaagacatcg acctcgtacc
     3361 ggtgcgggtg gagacgtggg gcggctgcgc cttcatcacc ttcgacgacc acgccccgcc
     3421 actgcgcgag tgcctgggcc cgttcgcccc gatggaagag atgcggcagg tcgaggatct
     3481 caaggtagcc ctgtggattg cgatccgcct gcctgccaac tggaagatgg cggccgaggc
     3541 cttcatggaa agctaccacg tcctgcgctc gcacccgcag ctggtctcga tgttcggcct
     3601 gacgatcgag ggcttcaacg aggctgccgc gcgcggaatc accagttc
//
LOCUS       DATNBV010000584         5548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_2555100,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000584 DATNBV010000000
VERSION     DATNBV010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2555100"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VN627_13760"
     CDS             complement(<1..1029)
                     /locus_tag="VN627_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04362.1"
                     /translation="MRVPPRKCSAPVNGTNQTSLAFVDPQRLGFRPNEPRRFSKDDIA
                     RACDVLRRFPPGLPLPIVVTEEYGVLVGHLLVHAAQKLGLTAITVIVHAGADPIDQNR
                     YSVAITQLLTKGSWDPADLETWVKQFEQQIEDFSHLDLGFGNGELDRILGFSDRLTAG
                     GAEADAGPQMHAVAVSRPGMVWHLGANRLMVGDATKTEHFAVLMAKLQAAMVITDPPY
                     GCPIDGFVSKKGLHRDFVEAAGEKAPEELKAFFLAFLRNLAGVMRPGALAYLFIDWRS
                     LSLLLDAGEAVFGSLVQLCCWTKDRGGMGGLYRSQHELVLVFRAPGAKHINNVELGRH
                     GRNRTNVWD"
     gene            complement(1095..1496)
                     /locus_tag="VN627_13765"
     CDS             complement(1095..1496)
                     /locus_tag="VN627_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04363.1"
                     /translation="MTQALEETDEGRGPGRPEIEDHAFLVGFLKSARQRGLTLSGAVR
                     EAKQMTQFGYERDPATGEHRLVAKRVLSAAGLRRRVHAALKEFTEAYDAAAPVLDGDA
                     ARYSSPRQPKPELLDFEPATRGRPRKLRPRN"
     gene            1725..1919
                     /locus_tag="VN627_13770"
     CDS             1725..1919
                     /locus_tag="VN627_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04364.1"
                     /translation="MSGLRALLGQPRGGERDRNPIPPDQPDDGKCLANADGVAAGDYP
                     QIPPPASPATAPAFAGTGLI"
     gene            complement(1936..2100)
                     /locus_tag="VN627_13775"
     CDS             complement(1936..2100)
                     /locus_tag="VN627_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04365.1"
                     /translation="MQRFWTRDFLMRRIDRCYLVACWARAADRRAAYLRLARHYRAIL
                     AGMAPAAAGV"
     gene            complement(2242..3552)
                     /locus_tag="VN627_13780"
     CDS             complement(2242..3552)
                     /locus_tag="VN627_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HXD04366.1"
                     /translation="MLAVAAPARADSAPDGLIIAGSVRLRYEAIDAQPRAGFNASDEL
                     VNLRTQLKASWKTGDVQFVAEMYDSRAWGANAGTPLSTGEVNTFEPVQAYLQADLGSA
                     LGRGTATKLQAGRFTLDFGSRRLVASDDYRNTTNSFTGLRADVTMPAGVKATAIYVLP
                     QTRLPDDAASLRANTPAIDKESFAAVLWGGLLARQSKGSRQLTEVSFIHFGEHDTPGR
                     ATRDRSLNNVALRLVTEPRPRQFDWGAEAIYQWGETATSLTPGAPLVPVSATFVRLHA
                     EYSFPGRWKPRLLFEFDRASGAGPGRTYGRFDPLFGMRRADLGPAGLYNAVGRSNILS
                     PGIRLEVTPSKRVDAFIGYRALWLADRHDAFSTSGVRDASGRSGAFAGHQVDARLRYW
                     LAPAKLRFELDGTLLARGHFLEAAPNGRTGTARYGSINLTGFFP"
     gene            3812..4816
                     /locus_tag="VN627_13785"
     CDS             3812..4816
                     /locus_tag="VN627_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXD04367.1"
                     /translation="MFIGAVAACAAMLGACSGSKSSGNAVELTNVSYDPTRELYQAIN
                     PAFAAAWKQKTGQEVTFRMSHGGSGKQSRAIIDGLDADVATLALAYDIDAIAAKAKLL
                     PGDWQRTLPNNSAPYTSTIVFLVRQGNPKGIHDWGDLIKPGVAVITPNPKTSGGARWN
                     FLAAWAYGRKAGGSDTAAEDYVRKLYAQVPVLDSGARGATTTFAERQIGDVLIGWENE
                     ALLARSKMGAGKFEVVVPPTSILAEPPVAVVTANAKKHGTEALARAYLEFLYTPEAQV
                     AIARNFYRPRDPQIAAQFKGQFPEVTLATIDKDFGGWAKAQHQFFDDGGVFDRIYARK
                     "
     gene            4847..>5548
                     /gene="cysT"
                     /locus_tag="VN627_13790"
     CDS             4847..>5548
                     /gene="cysT"
                     /locus_tag="VN627_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HXD04368.1"
                     /translation="MPSPKPLRHGWRTPSVIPGLPLTLGVTLTWLTLIVLIPLATLFL
                     KSAGMGWERFISVGFSERALAAYRLSFGTAAAAGLVNAIFGLLVAWALVRYAFPGKRL
                     ISAMVDLPFALPTAVAGIALTALYAPNGWIGRYLEPLGIKVAFTAIGITVALVFIGLP
                     FVVRSVEPVLADLGRDVEEAAATLGARRSQTFIRVILPAIFPALLTGFALAFARGVGE
                     YGSVIFIAGNMPGKTE"
BASE COUNT          960 a         1789 c         1856 g          943 t
ORIGIN      
        1 gtcccacaca ttggtgcggt ttcgcccatg gcggccgagc tcgacgttgt tgatatgctt
       61 tgccccaggt gcccggaaca ccagtaccag ctcgtgctgc gaccgatata ggccgcccat
      121 cccaccgcgg tctttggtcc agcagcacag ttgaaccaac gaaccgaaca ccgcttcccc
      181 ggcgtccaac agcaggctga ggctgcgcca gtcgatgaac aggtaggcca gcgccccggg
      241 gcgcatgacc ccggcaagat tgcgcaggaa agcgaggaag aacgctttca gttcctcggg
      301 tgccttctcg ccggcggcct cgacgaagtc gcggtgcagg cccttcttcg agacaaaacc
      361 gtcgatcggg caaccatagg ggggatcggt gatgaccatc gcggcctgca gcttggccat
      421 cagcaccgcg aagtgctcgg tcttcgtcgc gtcgccgacc atcagccggt tagcgccgag
      481 gtgccagacc atgccgggcc gcgatacggc caccgcatgc atctgcgggc cggcgtcagc
      541 ctcagcgccg ccggccgtca atcggtcgga aaacccgagg atgcggtcga gctcgccatt
      601 gccgaagcca agatcgagat gcgagaagtc ctcgatctgt tgctcgaact gcttgaccca
      661 ggtttcgagg tcagccggat cccagctgcc cttggtgagg agctgggtga tcgccaccga
      721 gtagcggttc tgatcgatcg gatccgcacc ggcatggacg atcaccgtga tcgcggtgag
      781 ccccagcttc tgcgccgcgt ggaccagcag atggccgacg agcacgccgt attcttcggt
      841 gacgacgatc ggcagcggca ggcccggggg gaagcggcgc agcacatcgc aggcgcgggc
      901 aatgtcatcc ttgctgaacc ggcgcggctc gttcgggcgg aagcccagcc gctgtggatc
      961 gacaaacgca aggctcgtct ggttggtccc gttgacggga gcggaacatt ttctcggtgg
     1021 aacgcgcatt cagccctccg tgattcggtg gagggcaagc aaatggacca cgaaaaatga
     1081 gcggataaag ccctttagtt gcgcggccgc aattttctcg gccggccgcg ggtagctggc
     1141 tcgaaatcga gcagctcggg cttaggctgg cggggcgagg agtagcgggc cgcatcaccg
     1201 tcaagcaccg gcgcagccgc atcgtacgct tcggtgaatt ctttcagcgc ggcgtggaca
     1261 cgccgccgca agcccgcagc cgagagcacg cgcttggcca cgaggcggtg ctcaccggtg
     1321 gccggatcac gctcgtagcc gaattgggtc atctgtttcg cttcccggac ggcgccgctg
     1381 agcgtcagtc cgcgctggcg tgccgacttg aggaagccga ccaagaacgc gtggtcctcg
     1441 atttccgggc gtccgggccc gcggccctcg tctgtttctt ctaaggcttg ggtcatggcg
     1501 cgattccctg ttgtggggcg cgaggctgga acagggaatg ggcggtgact agccccctcc
     1561 ccctgcggct ccgagccgga attgatgaga tttggaagcc cgcaattgcg ccgctgcggc
     1621 gcggcaaccg cccccttcga acctgattta acataagatc agttggcgta ccgggtcctc
     1681 aacagtgcgc tgggccaaac cgcaccagcg cgggccgctc tggcatgtcc gggttacggg
     1741 ctctgcttgg ccaaccgcgc ggcggcgagc gcgatcgcaa cccgatcccg cccgaccagc
     1801 cggacgatgg caaatgcctg gcgaatgctg acggcgtggc ggcgggcgat tacccgcaga
     1861 taccaccacc cgccagcccg gccaccgcgc ctgcgtttgc cggaaccggc ctgatttagc
     1921 accgccgtca cccgctcaga cccccgccgc cgccggagcc atgcccgcca gaatggcgcg
     1981 atagtggcgc gccaaccgca ggtaggcggc gcgcctgtcg gccgcgcgcg cccagcacgc
     2041 aaccagatag caacggtcga tccggcgcat gaggaagtcg cgggtccaga agcgctgcat
     2101 agttcactcc tctgaaattc gactgatttg gtagagaata atctccggcg tcgcaccatc
     2161 aagggctggg cgccgaggcg cggcctgctt gcgctgaagt gaccgccccg cccggccttc
     2221 gccggtgcag cagaccgggc atcaggggaa gaagcctgtg aggttgatcg aaccgtaccg
     2281 cgcggtgccg gtccgtccgt tcggcgccgc ctcgagaaag tggccgcggg ccaacagggt
     2341 gccgtcgagt tcaaagcgca gcttcgccgg agccagccaa tagcgcaggc gcgcatcaac
     2401 ctggtgcccg gcgaacgccc ctgagcggcc gctggcatcg cgcacaccgc tcgtcgaaaa
     2461 ggcgtcgtgg cgatcggcca gccacagggc ccgatagccg atgaaggcat cgacgcgctt
     2521 ggagggggta acctcgagcc ggatgcccgg cgacaggata ttcgaccggc ccacggcatt
     2581 gtagagcccg gccgggccga ggtcggcccg gcgcatgccg aacagcgggt caaagcggcc
     2641 ataagtccgt ccgggtccag cgccactggc gcgatcgaat tcaaacagca ggcgcggctt
     2701 ccaccggccg gggaagctgt attcggcgtg gaggcggacg aaggttgcgc tgacggggac
     2761 gagcggcgca ccaggcgtca gcgaggttgc ggtttcgccc cactggtaga tcgcttccgc
     2821 tccccagtcg aactggcggg ggcgcggctc ggtgacgagg cggagggcga cattgttcag
     2881 cgaccgatcg cgggtggccc ggcccggcgt gtcatgttca ccgaaatgga tgaagctgac
     2941 ctcggtgagc tggcggctgc ccttgctctg ccgggcgagc aggccgcccc agagcacggc
     3001 ggcgaagctt tccttgtcga tcgccggcgt attggcacgc agcgaggccg cgtcgtccgg
     3061 gagccgcgtt tgcggcaaga catagatcgc ggtcgccttc acgccggcgg gcatggtgac
     3121 gtcggcgcgc aggccggtga aactgttggt ggtgttgcga taatcatcgc ttgccaccag
     3181 gcggcgggag ccgaagtcga gggtgaagcg gccggcctgg agctttgtcg cggtgccgcg
     3241 gcccagggcg ctgcccagat cagcctgcag gtaggcctga accggctcaa aggtgttgac
     3301 ctcgccggtg ctcagcgggg tgccggcatt cgcgccccag gcccggctgt cgtacatttc
     3361 cgcaacgaac tggacgtcgc cggttttcca cgaggccttc agctgcgtgc gcaggttgac
     3421 cagctcgtcg ctcgcattga accccgcgcg tggctgggca tcgatcgcct cgtagcgcag
     3481 acgcacactg ccggcgatga tcaggccatc gggcgcggaa tccgcccggg caggcgcagc
     3541 aacagcgagc aagcaaacag gcaacagggt agcgtatcca gcaaacatgc ggcgtccttc
     3601 gatcgacaat ggacgcgatg cttgggcgca agtgcctgga ggggagatcg catccccgca
     3661 ggcgaatttg cgcaatcgca tttcaatgtc aagtcagcta gtagagtttg caatggactc
     3721 cggccgcctc tcagcatatc tcccacttga cggggacgac ccagcagcgg aggcgacaat
     3781 gttgaaattc acgcgggtgg tccggcgcac ggtgttcatc ggcgcggttg cggcttgcgc
     3841 ggcgatgctc ggtgcgtgtt cgggcagcaa aagcagcggc aatgcggttg aactgaccaa
     3901 cgtctcctac gacccgaccc gcgaactcta tcaggcgatc aatccggcgt tcgcggccgc
     3961 gtggaagcag aaaaccggcc aggaggtcac tttccggatg agccatggcg gttcgggcaa
     4021 gcagagccgg gcgatcatcg atgggctcga cgccgacgtg gcgacgctgg ccctcgctta
     4081 cgacattgat gcgatcgcgg cgaaggccaa gctgttgccc ggtgactggc agcgtacgct
     4141 gccgaacaat tcggcgccct atacctcgac catcgtcttc ctggttcgcc agggcaatcc
     4201 caaaggcatc cacgactggg gcgatctgat caagccgggg gtggctgtga tcaccccgaa
     4261 tcccaagacc agcggcggcg cgcgctggaa cttccttgcg gcctgggcct atggccgcaa
     4321 ggccggcggc tccgataccg cagccgaaga ctacgtccgc aagctctatg ctcaggttcc
     4381 tgtcctcgac agcggcgcgc gtggggcgac caccaccttt gccgagcgcc agatcggcga
     4441 cgtgctgatt ggatgggaga acgaagcgct cctggcccgg agcaagatgg gcgccggcaa
     4501 gttcgaggtc gtggtgccgc caacatcgat cctggcggag ccgcccgtgg cagtcgtaac
     4561 agccaatgcc aagaaacatg gaactgaagc gctcgcccgc gcctatctgg aattcctgta
     4621 cacgccggag gcccaagtgg cgattgcccg caacttctac cgcccgcgcg atccgcagat
     4681 tgcggctcaa ttcaagggcc agttccccga ggtgaccctt gctaccatcg acaaagattt
     4741 tggcggctgg gcgaaggcgc agcatcagtt cttcgacgac ggcggggtgt tcgaccgtat
     4801 ctacgcgagg aagtagggac actgaccgca cgtgagcagg cgcgagatgc cctccccgaa
     4861 gccactccga cacgggtggc ggacgccatc ggtcattccg gggctgcccc tgacgctcgg
     4921 cgtcaccctg acgtggctga cgctgatcgt gctgatccca ctcgcgacct tgttcctgaa
     4981 atcggcgggc atgggttggg agcgcttcat cagtgtcggt ttctccgaac gagctctggc
     5041 ggcctaccgc ctcagctttg gcaccgcggc ggccgctggc ctcgttaatg cgatcttcgg
     5101 actgctcgtc gcttgggcgc tggtccgcta tgcctttccc ggcaagcggc tgatctcggc
     5161 aatggtcgat ctgccatttg ccctgcccac cgctgttgcc ggtatcgcgc tgaccgcgct
     5221 ttacgcgccg aacggctgga tcggccgcta tcttgagcca cttggaatca aggtcgcctt
     5281 cacggcgatc gggatcacgg tcgcgctcgt gttcatcggc ctgccgttcg tcgtgcgctc
     5341 ggttgagccg gtgctggccg atctcggacg cgatgttgag gaggcggcgg cgacgctcgg
     5401 ggccaggcgt tcgcagacct tcattcgggt gatccttccg gcgatcttcc ccgcgctcct
     5461 cacgggcttt gccctcgcct tcgcccgcgg ggttggggaa tatgggtcgg tgatcttcat
     5521 cgcgggcaat atgccgggca agaccgag
//
LOCUS       DATNBV010000585        10437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4462586,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000585 DATNBV010000000
VERSION     DATNBV010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4462586"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VN627_13795"
     CDS             complement(<1..548)
                     /locus_tag="VN627_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04369.1"
                     /translation="MTEQSIPEWVEGDRFGLALPSRSPGLREGGAEFLTRAFHATGAL
                     APANRVRAITRLDEWSVGGTGAKAMVSVSYEREEPGLVHDLFVKFSRNFTDRTRDRVR
                     FHMEPEVKLANLSRDPAFPVPVPRCLFADFEHASGTGIIITERIPFGEGAVEPHHLKC
                     MDQRLPEPLDHYRTLIATLAHLS"
     gene            complement(628..1050)
                     /gene="ribH"
                     /locus_tag="VN627_13800"
     CDS             complement(628..1050)
                     /gene="ribH"
                     /locus_tag="VN627_13800"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXD04370.1"
                     /translation="MARILIVEARFYDHLNDMLVAGAKAAIKAAGHQAEVITVPGALE
                     IPGAIALADQGGSYDGYVAIGVVIRGETYHFEIVAGESARGIMALTMDGMAIGNGILT
                     VENEQQALVRADPKQKNKGGEAALAALALLSLREKFGA"
     gene            complement(1080..2360)
                     /gene="ribB"
                     /locus_tag="VN627_13805"
     CDS             complement(1080..2360)
                     /gene="ribB"
                     /locus_tag="VN627_13805"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HXD04371.1"
                     /translation="MSTIERVRTLVTTGQMTRAGLARAAGLHANTLRDCTDDDWNPTS
                     ETLGKLERFLLTNDDRPVLVSIEEIIDEARNGRMFVLVDDEDRENEGDLVIPAQMATP
                     AAINFMATHGRGLICLTLTGERCEQLGLELMSRHNGTRHETAFTVSIEAREGVTTGIS
                     AADRARTVSVAIDGTKSKADIVTPGHVFPLRAKDGGVLVRTGHTEAAVDISRLAGLNP
                     SGVICEIMRDDGTMARMDDLVTFARMHDLKIGTIRDLIAYRRKHDRMVEKRNELAFTS
                     KWGGDWKAITFYNKATGDETLALVKGRIDPEQPTLVRMHSMSFYADAFGEVGPRSDLL
                     SRSMEMIAEAGAGVIVVINRFMTGAISRAMEIKQQVREGIEPPMEELRDYGVGAQILA
                     ELGVHDMVLLTNTHHSLVALEGYGLSIVGERKIA"
     gene            complement(2483..3724)
                     /locus_tag="VN627_13810"
     CDS             complement(2483..3724)
                     /locus_tag="VN627_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXD04372.1"
                     /translation="MSTVNPLNGPLKGIRVLDLSRVLAAPFAAQMLSDMGAEVIKVER
                     PGLGDDSRRISPFLTNADGSRGESMFYLSANRGKRSITIDLSKPEGQELVRQLAADSD
                     VLIENYKVGDLKRYGLDYDAIRAINPRIVYCSVTGYGQNGPYAPKPGYDAIFQAMSGL
                     MSTNGAPAGEKGEGPTKVGVSMSDMLAGLFASSAICGALVDQARTGTGRYIDVALLDA
                     SFATLSHLAQWYLSLGQVPPRRGSEGNGGMPAGLFTCGDGKDLMITAGNQAQWLRLAE
                     AMDVMHLTEDPRFDTVFTRSDNRRALVPLLQEVFETRPLAYWIEKLDEAGVPAGPVYN
                     IAEALEDPQLKSRGPVVTTHHPSYGEMKLVANPVKFPGEQNTAEPLHPPLLGEHTDEV
                     LRDVLHFDDARIAELKAKGIV"
     gene            complement(3823..5118)
                     /locus_tag="VN627_13815"
     CDS             complement(3823..5118)
                     /locus_tag="VN627_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXD04373.1"
                     /translation="MPETLDAATDTAAHAHREQAERLAARVDDVPADTPLIPVDHVGI
                     IGAGTMGRGIALAFLNGGLRVTIVEMVPAALENAQKLMRKSYDGQLRRGQITQEEVEQ
                     RLARLTAAGTLDAVADCDLVIEAIFEDMALKLELFGKLDTIVKPGALLASNTSYLDID
                     AMAAATARPELFLGMHFFSPANIMKLLEIVRSAKTGNSAIATVMALTGRIGKVPVLSR
                     VCHGFIGNRMNRRRREAAERLMLEGAEPARIDAVVERFGFKMGPFAASDMAGLDVMWS
                     AAKSTGSTPKDVLCDAGRYGQKNGKGYYDYDENRNRTPSAEAVELIRGLARKLNVAQR
                     EFTDEEILGAVLYPAVNEGAKILDEGIAQRASDIDVTWLNGFGFPATEGGIMYWADRQ
                     GLDKVLAGMEQLEARYGEDYAVAPLLRRLAAEGRTFAEA"
     gene            complement(5190..5813)
                     /locus_tag="VN627_13820"
     CDS             complement(5190..5813)
                     /locus_tag="VN627_13820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HXD04374.1"
                     /translation="MPQGKLHMLVGKSALIINEMQLGVVDPAHAGFPGLANQVVERGI
                     AARIAQLAAAFRGAGLPVFHTPVVHRPDLADVKANALIHALTLKSGKMKEGSVEVRYV
                     PELQPAKGDFEIVRTSGMVSICATQLDAILRRMAIETVVITGVSTNIGVAGNAIVAGE
                     LGYHVVVPEDCIAGSDPDVHKILVREQLRMVARIVTADDVISSLPAG"
     gene            6032..6865
                     /locus_tag="VN627_13825"
     CDS             6032..6865
                     /locus_tag="VN627_13825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXD04375.1"
                     /translation="MKARFPKFDFSQVKAHWAPSAEFSQMFNASSLIPAYVEPYLLKV
                     MRMAAGKIDPSDTKLLEEIDIFCKQEMQHCKYHIAYNKVIRGQGYEGLKPLEDKIAAE
                     YDDWLKNRSLRFHLSYCEGFESMSATACEAWFESFDDLVDGADPAVLDMWRWHLAEEF
                     EHRTVCSDAYHALSGLNPVSRYFYRVYGYIYAMAHLGKFAGAATKYLLEQDRAKMTPE
                     EVAASIARQKAVKKRVTKGILPMMLKIMSPFYDPAKRRDPVGSREFLEEFEQRRAALA
                     A"
     gene            6969..7790
                     /locus_tag="VN627_13830"
     CDS             6969..7790
                     /locus_tag="VN627_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXD04376.1"
                     /translation="MQDGEWVFMYDVSAEVAQAQEILCHDVDGTVVVTLNAPQFRNAL
                     STEMRELLLERLQALTSSADCRAIVVAGAGGHFCSGGRLPPNVAPDPDRSRRNGVFLQ
                     EIARILHLGPKPTIAAVEGTAFGAGFAIAMACDRVVAAEGARMCAAFGKVGLVADTGI
                     AYTLSQRIGSHHARDLLLTGREVRGAELIELGLVDRVVPAEDVLSAALTEAGRYAQVA
                     PLSLAATKRMLGQCQSLDAVFAAESAELPPLTMTHDYAEGRSALREKRKPVFRGA"
     gene            7819..9006
                     /locus_tag="VN627_13835"
     CDS             7819..9006
                     /locus_tag="VN627_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXD04377.1"
                     /translation="MDLLIDDKHLALRDEMRAFFAKHLPADFSRRTLAGDKLTKDEHN
                     HWHALLREKGWNVPLWPKEHGGPGWGPMERFIWEEESGLAGAPRLNHVSQDLLGPVLI
                     EFGTPEQKAELLPRILSSEDWWCQGFSEPQAGSDLANLQMRAVRDGDDYIVTGTKLWT
                     SYAHLSNKIFCLVRTSADGPKQAGISFLLVDMEQPGVTVSPIYTLGHMLSVNEVQIEE
                     VRVPVTNLVGREGGAWDITKFLLGNERLMGAWIGVNMAFARQVRERLEQVGPDGRKRS
                     ENAVLAQRVAEVETDLLALRYTTYRVLADELSGKAPGPEVSVIKVRGGEIMQSLTELL
                     MEMAEMDGLVHPYHLPHGGLPVVPLELEHMAQQFFDRRKLTIYGGSAEIQRNIIARRL
                     LNV"
     gene            9020..10156
                     /locus_tag="VN627_13840"
     CDS             9020..10156
                     /locus_tag="VN627_13840"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXD04378.1"
                     /translation="MDFNYSDNQRMLSDMVDRFLTDKHDLPTRQSLVTDPAADAALWR
                     ELAELGLIGAAFPESVGGYGESSADIYVVMERFGRHLVTQPFLYTAVICGRLLIEGLS
                     GDQRDGLIAEVIAGERLLALAACSTHALRSPQDTSFTATKAGNGWTLTGRMPVVMGGD
                     RANTFLVAARTSGELGDRDGVTLFMVDAQAPGLTRRALRTIDLFGAAELVLEGVEVAA
                     DAIVGTEGGAVPLIELALDHGVAAVCAEAIGSMEYLIPTTAEYTRTRIQYGAPLAKFQ
                     VLQHRMADMYIQTQTAKSIAMVAAMSLDGDPVERQRLVSAAKAQVGRSAKFVGYQSVQ
                     LHGGMGVSEELDIGHHYIRLNVINQLLGDGSFHLRRFGTLAAAA"
BASE COUNT         1759 a         3330 c         3455 g         1893 t
ORIGIN      
        1 gaaaggtgcg cgagcgtggc gatcagcgtg cggtaatggt ccagcggctc gggcaggcgc
       61 tggtccatgc acttgaggtg gtgcggctcc accgcgccct cgccgaaggg gatacgctcg
      121 gtgatgatga tcccggtgcc cgaggcatgc tcgaaatcgg caaacaggca gcggggcacc
      181 ggcacgggga aggccggatc gcgcgacagg ttggcgagct tgacctccgg ctccatgtga
      241 aaccgcaccc ggtcgcgggt gcgatcggtg aaattgcgcg agaacttgac gaacaggtca
      301 tgcaccagac cgggctcttc gcgctcgtag gagaccgaca ccatggcctt ggcaccggtt
      361 ccgcccacgc tccattcgtc gaggcgggtg atggcccgca cccggttggc aggggcaagc
      421 gcgccggttg catggaaggc ccgggtgaga aactccgctc cgccttcgcg cagccccggc
      481 gagcgggagg gaagcgccag cccgaaccga tcgccctcaa cccattccgg aatgctctgc
      541 tctgtcatcg ccgatgcgct agcgcggggg aggggacagg agcaagcagc cgccgagcct
      601 gcgcgcgact gtgatgcgtg gctgcaatca ggcgccgaat ttttcgcgca ggctcaagag
      661 cgccaaggcg gccagagcgg cttcgccacc cttgttcttc tgcttgggat cggcgcggac
      721 tagcgcctgc tgttcgttct cgacggtgag gatgccattg ccgatggcca tgccgtccat
      781 ggtcagggcc atgatcccgc gcgcgctttc gccggcgacg atctcgaagt gataggtttc
      841 cccccggatc accacgccga tggcaacata gccgtcatag ctgccgccct ggtcggcgag
      901 ggcgatcgcg ccggggattt ccagggcccc gggcacggtg atcacctcgg cctggtggcc
      961 ggcggccttg atggcggcct tggctccggc gaccagcatg tcgttcagat ggtcgtagaa
     1021 acgcgcctcg acgatgagga tgcgggccat gtcaggtatc cttggaaaag aggtgtgcgt
     1081 caggcgatct tgcgttcgcc gacgatggac aggccatagc cttccagggc gaccagcgaa
     1141 tggtgggtgt tggtcagcag caccatgtcg tggacgccca gttcggcgag gatctgggca
     1201 cccacgccat agtcgcgcag ttcctccatg ggcggttcga tgccttcgcg gacctgctgc
     1261 ttgatctcca tggcccggct gatggcgccg gtcatgaacc ggttgatcac cacgatgact
     1321 cccgcgccag cttcggcgat catctccatc gaccggctga gcaggtctga ccgggggccg
     1381 acttcgccga aggcatcggc atagaaagac atgctgtgca tccgcaccag ggtcggctgt
     1441 tcgggatcga tccgcccctt gacgagggcc agcgtctcat cgccagtcgc cttgttgtaa
     1501 aaggtgatgg ccttccagtc tccgccccac ttggaggtga aggccagttc gttgcgcttc
     1561 tcgaccatgc ggtcatgctt gcggcgatag gcgatcagat cgcggatcgt gccgatcttg
     1621 agatcgtgca tccgcgcgaa ggtgacgagg tcgtccatcc gcgccatcgt tccatcgtcg
     1681 cgcatgattt cgcagatcac gcccgaggga ttgaggcccg ccagccgcga gatatccact
     1741 gccgcctcgg tatggccggt gcggaccagc acgccgccat ccttggcgcg cagcgggaag
     1801 acatgccccg gggtgacgat atcggccttg ctcttggtgc catcaatggc gacgctgacc
     1861 gtgcgggcgc ggtcggcggc ggagatgccg gtggtcaccc cctcgcgcgc ctcgatcgaa
     1921 acggtgaagg cggtttcatg ccgggtgccg ttgtggcggc tcatcagttc gaggccaagc
     1981 tgctcgcagc gctctcccgt cagcgtgagg cagatcaggc cccgcccgtg ggtggccatg
     2041 aagttgattg ccgccggcgt cgccatctgt gccgggatta cgaggtcgcc ctcgttctcg
     2101 cggtcctcgt cgtcgaccag cacgaacatt cgcccgttgc gagcctcatc gatgatttcc
     2161 tcgatcgaga cgagcacggg ccggtcgtcg ttggtcaaga ggaagcgctc cagcttgccc
     2221 agcgtttcgg atgtgggatt ccagtcgtca tccgtgcagt cgcgcagggt attggcgtgg
     2281 agcccggccg cgcgggcaag gccggcgcgg gtcatctggc cagtggtgac gagggtgcgg
     2341 acgcgttcga tcgtggacat ggcccacctt atatcacccc cgcgatcacc ttgcaatgtg
     2401 gccacacatt aaagtgtgac gcgccgggcg cggtatgttg atcgtgggta gaatgacccg
     2461 tgcgttcccg ccgtaatccg gcctagacga tgcccttggc tttcagctcg gcaatccggg
     2521 catcgtcgaa gtgcagcacg tcgcgcagca cctcgtcggt gtgctctccc agcaggggcg
     2581 ggtgcagcgg ttcggccgtg ttctgctccc ccgggaactt caccggattg gcgaccagct
     2641 tcatctcgcc gtaagacgga tggtgcgtcg tcacgactgg cccgcgcgat ttgagctgcg
     2701 ggtcctccag cgcctcggca atgttgtaga cggggccggc gggcaccccg gcttcatcga
     2761 gcttctcgat ccagtaagcg agcggcctgg tctcgaacac ttcctgcagc agggggacga
     2821 gcgcgcggcg gttgtcgctg cgagtgaaca ccgtgtcgaa gcgcgggtcc tcggtcaggt
     2881 gcatgacgtc catcgcttcg gcaaggcgca gccactgagc ctggttgccg gcagttatca
     2941 tcaggtcctt gccgtcaccg caggtgaaca gccccgcggg cattccgcca ttgccttccg
     3001 agccgcgccg tggcggcacc tggccgagcg agagatacca ctgcgccagg tgcgacagcg
     3061 tggcgaagga agcatcgagc agcgccacgt cgatatagcg gccggtcccg gtgcgggcct
     3121 ggtcgaccag ggcgccgcag atagccgagc tggcgaacag gcccgccagc atgtcggaca
     3181 tcgagacgcc aaccttggtc gggccttcgc ccttttcgcc ggcgggagcg ccgttagtgc
     3241 tcatcaggcc gctcatcgcc tggaagatcg cgtcgtagcc gggcttggga gcataggggc
     3301 cgttctggcc gtagccggtc accgagcagt agacgatgcg cgggttgatc gcgcggattg
     3361 cgtcatagtc gaggccgtac cgcttgaggt cgcccacctt gtaattctcg atcagcacgt
     3421 cgctgtcggc ggcaagctgc cggaccagtt cctggccttc gggcttcgac aggtcgatgg
     3481 tgatcgagcg cttgccgcgg ttggccgaga ggtagaacat cgattcgccg cgcgacccgt
     3541 cggcattggt caggaagggg ctgatccggc ggctgtcgtc gccaagcccc ggccgctcca
     3601 ccttgatcac ctcggcgccc atgtccgaca gcatttgcgc cgcgaagggc gccgccagca
     3661 cgcggctgag gtccagtacg cggataccct tgagcgggcc attcagcggg ttgaccgtcg
     3721 acacttgcta ctccgttagc tctcgaaaaa tgaattgcct gcctggcaga tagaacggtg
     3781 gcccgccgat ctcaacggcg gggcgccgca attgcagctg cctcatgcct cggcgaaggt
     3841 gcgcccctcg gcggccagcc ggcgcagcag cggggccacc gcgtaatcct cgccatagcg
     3901 cgcttcgagc tgctccattc cggccaggac cttgtccagc ccctgccggt ctgcccagta
     3961 catgatgccg ccctcggtcg ccgggaatcc gaagccgttg agccaggtga cgtctatgtc
     4021 ggaagcgcgc tgggcaatgc cttcgtccag gattttggcg ccttcgttga ccgcagggta
     4081 aagcaccgcg cccaggatct cctcgtcggt gaattcgcgc tgggccacgt tcagcttgcg
     4141 cgcaagcccg cggatcagtt cgacggcttc ggccgaaggg gtgcggttgc ggttctcgtc
     4201 gtagtcgtaa tagcccttgc cgttcttctg gccgtagcga ccagcgtcgc acagcacgtc
     4261 cttgggggtc gatccggtcg atttggcggc tgaccacatg acgtccagcc cggccatgtc
     4321 ggacgcggcg aagggcccca tcttgaagcc gaaccgctca accacggcgt cgatccgcgc
     4381 aggctcggcg ccctccagca tcagccgctc tgccgcctcg cggcggcgcc ggttcatccg
     4441 gttgccgatg aagccatggc acacgcgcga aagcaccggc accttgccga tccgcccggt
     4501 caaggccatc accgtggcaa tggcgctgtt gccggtcttc gcgctgcgca cgatctcaag
     4561 caatttcatg atgttggcgg gcgagaagaa gtgcatgccc aggaacagct ctggccgcgc
     4621 ggtggcagcg gccatggcgt cgatgtcgag gtacgaggtg ttgctggcca gcagcgcgcc
     4681 gggcttgacg atcgtgtcga gcttgccgaa caactcgagc ttgagcgcca tgtcttcgaa
     4741 gatcgcctcg attacgaggt cgcagtccgc cacggcgtcg agcgtgccgg ccgccgtcag
     4801 ccgggccagc cgctgctcca cttcctcctg cgtgatctgg ccccgacgca gctgcccatc
     4861 gtagctcttg cgcatcagct tctgcgcgtt ttccagcgct gccgggacca tctcgacgat
     4921 ggttacccgc aacccgccgt tgagaaaggc caaagcgatg ccccggccca ttgtgcccgc
     4981 gccgatgatg ccgacgtgat ccaccgggat caacggcgtg tcggccggaa cgtcatccac
     5041 gcgggcggca agccgctcag cctgttcgcg gtgggcgtgg gcggcggtat cggtggcggc
     5101 gtcaagggtt tcgggcatcg ggaagtcttt caagcgatgt gccggcgagt caggcgcgcc
     5161 ggtctggagg agccatgcgg cctgggcggt cagccggcgg ggagggacga gatcacatcg
     5221 tcggcggtaa cgatccgggc gaccatgcga agctgttcgc gcacgagaat cttgtgaacg
     5281 tccgggtcgg aaccggcaat gcagtcttcc gggaccacga catggtagcc cagctcgccc
     5341 gcgacaatgg cgttcccggc caccccaatg ttggttgata ccccggtgat caccacggtc
     5401 tcgatggcca ttcggcgcag tatggcgtcc aattgggtgg cgcaaattga caccatcccc
     5461 gacgtacgaa cgatctcgaa atcgcccttc gcgggctgaa gctcgggaac gtaccgcact
     5521 tcgacgctgc cttccttcat cttgccgctc ttgagggtga gggcatggat gagagcattg
     5581 gccttgacgt cggcaagatc ggggcggtgg accacaggcg tatggaagac cggcaggccc
     5641 gcgccgcgga aggccgcggc tagctgcgct atccgcgccg cgatccctcg ttcgaccacc
     5701 tggttggcga ggccaggaaa tccggcatgg gcaggatcga ccacgccgag ctgcatctcg
     5761 ttgatgatca gggcagactt acctaccagc atgtggagtt tcccctgtgg cacggtgcag
     5821 cctctgcggc gatccggggg ctaaaggcaa acaccgccgg agagccatca catggttgat
     5881 ccccgcgccg tgaggcgtga ggggtgtgat catgcacttt ggggttgtcg cggcgcataa
     5941 gcgatcacac atgtgaaaaa tcgtggtatg atccccgcat aggatgccgg atgggatatc
     6001 gggcgtccat tgctaccgag agaggctgct catgaaagcc cgttttccga agttcgattt
     6061 cagtcaggtg aaggcgcatt gggctcccag tgcggagttt tcgcagatgt tcaacgcgtc
     6121 gtcgctgatc cctgcctatg tcgagcccta cctgctcaag gtaatgagga tggccgccgg
     6181 aaagatcgat ccctccgaca cgaagctgct cgaggaaatc gacatcttct gcaagcagga
     6241 gatgcagcac tgcaagtacc atatcgccta taacaaggtg atccgcggcc agggctacga
     6301 agggctcaag cccctggagg acaagattgc ggcggagtac gacgattggc tgaagaaccg
     6361 ttcgctgcgc ttccatctgt cgtactgcga aggctttgag tcgatgagtg ctacggcttg
     6421 cgaagcctgg ttcgagagtt tcgacgatct cgtcgacggt gcggatccgg cagtgctcga
     6481 catgtggcgg tggcatctgg ccgaggagtt cgagcatcgc accgtgtgtt cggatgccta
     6541 tcacgccctc agcggcctga acccggtctc gcgctacttt taccgggtct atggttacat
     6601 ctatgcgatg gcgcacctcg gcaagtttgc cggcgcggcg acgaagtacc tgcttgagca
     6661 ggaccgcgcc aagatgaccc cggaagaggt cgcggcatcg atcgcgcgtc agaaggccgt
     6721 gaagaagcgc gtgaccaaag gcattctgcc gatgatgctc aagatcatgt cgcccttcta
     6781 cgatccggcc aagcgccgcg atccggtcgg ctcgcgcgag tttctcgagg aattcgagca
     6841 gcgccgcgcc gcattggcgg cctaggctcg cggtcggcac atacatgata acccgagccc
     6901 gacaggtgcg tgcagcggac ggtctcgcct aaaaggctgt cgaccggccc cgtcgtgtgg
     6961 gccgaggcat gcaagacggg gaatgggttt tcatgtatga cgtgagcgcg gaagtagcgc
     7021 aggcgcagga aatcctgtgt cacgacgtgg acggcactgt tgtcgtcacg ctcaatgctc
     7081 cgcaattccg caacgccctg tcgacggaaa tgcgcgaatt gctgctggag cggctgcagg
     7141 cgctgaccag ttcggcggac tgccgcgcca tcgtggtggc cggcgcaggc gggcacttct
     7201 gctctggtgg acggctgcct cccaatgttg ctccggatcc cgaccggagc cggcgcaatg
     7261 gcgtgttcct tcaggaaatt gcccgcatcc tgcacctcgg tccgaagcca acgatcgcag
     7321 cggtggaggg taccgcgttc ggtgccggat tcgccattgc catggcctgc gaccgggtgg
     7381 tcgcggcgga gggagcccgc atgtgtgcgg ccttcggcaa ggtggggctc gtggccgata
     7441 ccggcatcgc ctacaccttg tcgcagcgca tcggcagcca tcacgctcgt gacctgttgc
     7501 tcactggccg tgaagttcgc ggtgcggaat tgatcgagct tggcctggtc gaccgcgtcg
     7561 tcccggccga ggacgtgctt tccgcggctc tcaccgaagc gggccgctat gcccaggtcg
     7621 ccccgttgtc cctcgcggcg accaagcgca tgctcggcca gtgccagtcg ctcgatgcgg
     7681 tgtttgcagc cgaatcggca gaactgccgc cattgaccat gacacacgac tatgcggagg
     7741 gccgcagcgc cctgcgcgag aagagaaagc cggttttccg cggcgcctga tacgcctgac
     7801 cggcgaggag ttgatcaagt ggacctgtta atcgacgaca agcacctggc cttgcgcgat
     7861 gaaatgcgcg cattttttgc caagcacctg ccggccgact tttcgcggcg cacgctggcc
     7921 ggggacaagc tcaccaagga cgagcataat cactggcatg cgctgttgcg ggaaaaaggc
     7981 tggaacgtgc cgctctggcc aaaggagcat ggcggcccgg gctggggccc gatggagcgg
     8041 ttcatctggg aagaggaatc cggccttgcc ggtgcgccgc gcctcaacca cgtttcgcag
     8101 gacctgctcg gcccggtcct gatcgagttc ggcacgcccg aacagaaggc cgagcttttg
     8161 ccgcgcattc tctccagcga ggactggtgg tgccagggct tctccgagcc gcaggccggt
     8221 tccgaccttg ccaacctgca gatgcgcgcc gtgcgtgatg gcgatgacta tatcgtcacc
     8281 ggcaccaagc tgtggaccag ctacgcgcat ctttcgaaca agatcttctg cctcgtgcgt
     8341 acctcggccg acggtcccaa gcaggcgggc atttccttcc tgctggtcga catggagcag
     8401 cctggagtca ccgtgagccc gatctacacg ctcggccaca tgctctcggt caatgaggtc
     8461 cagatcgagg aggtgcgggt cccggtcacc aatctcgtcg gccgggaagg cggggcctgg
     8521 gacatcacca agttcctgct cggcaacgag cggctgatgg gcgcgtggat cggcgtgaac
     8581 atggcctttg cccgccaggt gcgcgaacgg ctcgagcagg ttggccccga cggccgcaag
     8641 cgcagcgaga acgcggtcct cgcccagcgc gtggccgagg tcgagaccga cctcctggcc
     8701 ctgcgctaca ccacctaccg cgtgctggct gacgagctct cgggcaaggc gcccgggccg
     8761 gaggtctcgg tcatcaaggt gcgcggcggc gagatcatgc aatcgcttac cgagctgctg
     8821 atggaaatgg ccgagatgga cggcctggtt catccctacc acctgccgca tggcgggctg
     8881 ccggtcgtgc cgctcgaact ggagcacatg gcccagcagt tcttcgaccg gcgcaagctc
     8941 acgatttatg gcggcagcgc ggaaatccag cgcaacatca ttgcgcgccg gctgcttaac
     9001 gtctgacggg aacagggcga tggacttcaa ctatagtgat aaccagcgaa tgctgtcgga
     9061 catggtcgac cgcttcctga ccgacaagca cgacctgccg acccgccagt ccctggtgac
     9121 ggacccggcg gccgatgccg ccctctggcg cgaactggcc gaactgggcc tgatcggcgc
     9181 cgccttcccg gaaagcgtgg gcggctacgg cgaatcgagc gccgatatct atgtggtgat
     9241 ggaaaggttc gggcgtcatc tcgtcaccca gccgttcctc tacaccgcgg taatctgcgg
     9301 ccggctgctg atcgaggggc tctccggcga ccagcgcgac ggcctcattg ccgaggtcat
     9361 cgcgggcgaa cgcctgctgg cgctggcagc ctgttcaacc catgccctgc gcagcccgca
     9421 ggacaccagt ttcaccgcca cgaaggcagg caacggctgg accctgacag gccgcatgcc
     9481 cgtcgtcatg ggaggtgacc gggccaacac gttcctcgtc gcggcgcgca cgtccgggga
     9541 actgggggac cgggatggcg tcaccctgtt catggtcgat gcgcaagcgc ccggcctcac
     9601 ccgccgggcc ctgcgcacga tcgacctgtt cggcgcggct gagctggtgc tcgagggggt
     9661 cgaggttgcc gctgacgcta tcgtcggcac cgagggcggg gcggtcccgc tcatcgaact
     9721 ggcgctcgat catggcgtcg ccgcggtctg cgccgaggcg attggctcga tggaatacct
     9781 gatcccgacc acggcggaat atacccgcac ccgcatccag tatggcgcgc ccctggccaa
     9841 gttccaggtg ctccagcacc gcatggccga catgtacatc cagacccaga ccgccaagtc
     9901 gatcgccatg gttgccgcca tgtcgctcga cggtgatccg gtggagcgcc agcggctggt
     9961 ttcggcggcc aaggcccagg tgggccgctc ggccaagttc gtcggctacc agtcggtcca
    10021 gttgcatggc ggcatgggcg tctcggagga gctcgatatt ggccaccact acatccggct
    10081 gaacgtcatc aaccagctgc tgggcgacgg ctcgttccac ctcaggcgct tcggcacgct
    10141 cgccgccgcc gcctgacccg gggctccgat cccattcgag accccgctcg gcacgctcgc
    10201 cgggcggggt ttcttgtgcc tgccacaata tctaatgaga atcagttgca atagactcgt
    10261 cttcgcgtct aggtggtcgt gcgttgcgaa tcgttcgcaa tacatcgacg cgtgggaatg
    10321 gaccgaatga gcaaggggct ggattactgc cgaccgtgga tcaccgcagc ggcaatgctg
    10381 atcgccgcgc cggccgcagc ggcaagtccc gccatgccgg ccgacctgac cgtcggc
//
LOCUS       DATNBV010000586         8890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4466763,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000586 DATNBV010000000
VERSION     DATNBV010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4466763"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VN627_13845"
     CDS             <1..637
                     /locus_tag="VN627_13845"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXD04379.1"
                     /translation="RPAGAAKAAIIVIPEIFGVNPGIRQKADHFASQGYLAVSPDVFW
                     RQEEGIELDADVPEQLQRAFGLFGGHDFDAGIRDVEATIRWIRREAGVPKVGLVGYCM
                     GGKVAYQAATRTDIDATVGYYGVGIADMLNESHAIARPLLLHVPMADGFVPPEQQKAM
                     HEGLDPNPHVTIHDYPGLDHGFAAEMGLRRNEEGARLADSRTEAFFAENLA"
     gene            complement(701..1957)
                     /gene="rho"
                     /locus_tag="VN627_13850"
     CDS             complement(701..1957)
                     /gene="rho"
                     /locus_tag="VN627_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXD04380.1"
                     /translation="MHLKELKKKTPAELVEMAEEFGVEGASTMRRQDLMFAILKEVAE
                     DGEEILGIGTIEVLTDGFGFLRSPEANYLAGPDDIYVSPNQVRKWGLRTGDTVEGEIR
                     APKDGERYFAINKLISVNFDDPDAVRHRVNFDNLTPLYPDERLRLDTIDPTVKDKSAR
                     VIDLISPQGKGQRALIVAPPRTGKTVLLQNMAKAITDNHPEVFLIVLLIDERPEEVTD
                     MQRSVRGEVIASTFDEPATRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLGRAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIATALIDTGSRMDEVIFEE
                     FKGTGNSEIVLDRKVSDKRIFPALDVGKSGTRKEELLVEKANLSKMWVLRRILMQMGT
                     VDAMEFLLDKIKDTKTNEDFFATMNQ"
     gene            2255..4468
                     /locus_tag="VN627_13855"
     CDS             2255..4468
                     /locus_tag="VN627_13855"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXD04381.1"
                     /translation="MDFEHRLNEAREQFHAGQLQTALEIVSALFESHGERGDTLNMAA
                     LCHLHGGDQAGALGLFRRAVAADPAYSPAWSNLGNLLRTLGAEAEGVAALRQAVAVDP
                     DCIEGWNNLGLAAMVAGQAAEAEQLFRRALEIHPGYAVSLFNLGRALLMTHRSEESEA
                     VLRRLLAAAPDYPFGQACLASALIDQGRTEEGEAAYRRELDRDPDNSVALNNLGLLAQ
                     NQRRLHEALGWLERAAMLPGADALTLSNYANVLREVGRPDDAAQWYRTAAQRDPEFTT
                     ALSKWMLSARAVCDWGDLTELEARIAEHIARGIVEAPAAFDLLVHPGLTDAQHRAVAE
                     AYGARAYGAALARPPLVPPGRPLHSGGGPLRIGYVSSDFYEHATMRLLRGVLAGHDPA
                     RVQFLCYSTGAIIGDAERERIEAAPACAGFRDLARLPDEAAACQIRDDAIDILVDLKG
                     YTQENRLGIQALRPAPVVVSWLGYPGTLGVARLADYIIGDPVVTPLQAQADYSEALAL
                     MPHCYQPNDDTMVTEAPPSRASQGLPDDGPVFCNFNAAYKFSPAAFASWMHLLRATPG
                     SVLWLLRPGEAARARLIAAAAAEGIAAERLVFAAPLSQRDHLARLQLADLALDTFPYT
                     SHTTGSDALLAGVPLVTRPGDTFASRVAASLLTAVGLPELIADDDEAARQLVLDLARD
                     GARRAALRAHLLAVRRNCPLFDTSGFTRDLERLYHAIGEQLQRGERRGFALSAEP"
     gene            complement(4473..5249)
                     /locus_tag="VN627_13860"
     CDS             complement(4473..5249)
                     /locus_tag="VN627_13860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXD04382.1"
                     /translation="MQELELYTACPLCDCTDLADLRTASCAGHGLYNPALNDTLLWRT
                     CAACGHQFRSGFYTDEACQLLFSGTHDNQRLGHDYEAQRWVAARMIEKVLPWRSSGPW
                     LDVGFGNGALLLTALEYGFTPVGIDLRTDNAAALAGLGIETHARPLETLEFPGRFSVI
                     SLADVLEHTPFPRLVLSHAHSMLASGGVLFLSMPNAESPLWHLLDAAGANPYWGELEH
                     YHNFTRTRLEALLRQHGFTPMRYGVSERYRACMEVIALKN"
     gene            complement(5272..5715)
                     /locus_tag="VN627_13865"
     CDS             complement(5272..5715)
                     /locus_tag="VN627_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HXD04383.1"
                     /translation="MQQALAMTYLWLKAGHVIFVIFWMAGLFMLPRYFVYHQEAPAGS
                     PERAVWIDRERKLLRIILWPSLIITWLLGLALAMQIGAFQQGWFHVKLAFVLALTAYH
                     LLLASYAAALARGEERLTGKRLRLLNEVPGIAAAFIVVMVIVKPF"
     gene            complement(5834..5989)
                     /locus_tag="VN627_13870"
     CDS             complement(5834..5989)
                     /locus_tag="VN627_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04384.1"
                     /translation="MKRTRLSICQRKVRYPSFAEARSVALAFPGAARPYRCDKCRRFH
                     LTSRRRT"
     gene            complement(6097..7122)
                     /gene="hemE"
                     /locus_tag="VN627_13875"
     CDS             complement(6097..7122)
                     /gene="hemE"
                     /locus_tag="VN627_13875"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HXD04385.1"
                     /translation="MPGLLFDTLRGTNGSRRPVWFMRQAGRYLPEYRELRARKGGFLP
                     LVYDSEAAAEITLQPLRRFGFDGAILFSDILIVPYAMGQDLEFLAGEGPRMSPRLVDH
                     ALSALESVPERLSPIYETVRLVRAALTPEQTMLGFAGSPWTIATYMVAGEGSRDQHET
                     RAYAYRDRGAFQAIIDAVVATTVDYLEGQVRAGAEAVQLFDSWAGSLAPAEFERWVIA
                     PNAEIVRQLKARCPDTPIIGFPKGAGEKLVAYARETGVDALGVDETIDPLWAARELPA
                     GLPVQGNLDPLLLLAGGDELDSQARRVLDAFADRPHVFNLGHGIGQFTPIEHVERLLK
                     VVRGWSR"
     gene            7438..8289
                     /locus_tag="VN627_13880"
     CDS             7438..8289
                     /locus_tag="VN627_13880"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HXD04386.1"
                     /translation="MPTTRLHLHLLSDSTGETLEMIAKAALAQFDDADVTRHFWPMVR
                     SQQHLDRILTEIAANPGLVLFTLVNAEIRVRLEDRCRALGLPVVPALDAVSNALEDML
                     GQQAKARPGRQHMLDEAYFARVDAIQFTIAHDDGLQWEDWEEADILLAGVSRSSKTPT
                     AIYLANRGYKVANIPIVPESPPPPVLFQLGRPLVVGLTTAPERLVQVRRNRLLSLNQA
                     PSTHYVDQERVAGEVQFARRMFADNGWPVIDVTRRSIEETAAAVIRLHQEHRARGETA
                     GAGSRAI"
     gene            8305..8886
                     /locus_tag="VN627_13885"
     CDS             8305..8886
                     /locus_tag="VN627_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HXD04387.1"
                     /translation="MLILASQSASRRAMLEAAGVPFMAVPAEVDERALEASLAGAAPD
                     VVATALARAKAEAVAARHPGRWVLGSDSLVVCEGRRFDKPANREAAAEHLRFFSGKTM
                     ELHSAATLVRDDAVIPCADKAELAVRELSEEFIADYLAHEWPAVAGCVGVFRIEGRGV
                     QLFTQLRGDHFTILGMPLLPVLAGLRSVGVMAA"
BASE COUNT         1466 a         2825 c         3115 g         1484 t
ORIGIN      
        1 gcggcccgcg ggcgcggcga aggctgcaat catcgtcatc cccgagatct tcggggtgaa
       61 ccccggcatc cgccagaagg ccgaccactt cgccagccag ggatatctcg ccgtctcgcc
      121 cgacgtgttc tggcggcaag aggaagggat cgagctggat gccgatgtcc ccgaacagct
      181 ccagcgggcg ttcggcttgt ttggcgggca cgatttcgat gcgggcattc gcgacgtcga
      241 ggcgaccatt cgctggatcc ggcgcgaggc gggcgtgccc aaggtcggcc tcgtcggcta
      301 ttgcatgggc ggcaaggtgg cctaccaggc ggcgacgcgg accgacatcg atgcgacggt
      361 gggctactac ggcgtcggca ttgccgacat gctgaacgaa agccatgcga tcgcccggcc
      421 gctgctgctc cacgtgccga tggccgatgg cttcgtcccg cccgagcagc agaaggcgat
      481 gcatgaaggg ctggacccca acccacatgt gacgatccac gactacccgg ggctggacca
      541 cggctttgcc gccgagatgg gcctgcggcg caatgaagaa ggcgcgcggc tggccgacag
      601 ccggaccgag gcgttcttcg cggagaacct cgcctgacgg gcatggccgc ccgaggctga
      661 acaaagaaaa agggcgcgga gtgatccgcg ccctttctgg ttactggttc atcgtggcga
      721 agaaatcttc gttggtcttg gtgtccttga tcttgtcgag caggaactcc atcgcgtcga
      781 ccgtccccat ctgcatgagg atccggcgca ggacccacat cttggagagg ttggcctttt
      841 cgaccagcag ctcttccttg cgggtgcccg acttgccgac atccagcgcc gggaagatgc
      901 gcttatcgga aaccttgcgg tcgagcacga tttcgctgtt gccggtgccc ttgaactctt
      961 cgaaaatcac ttcgtccatg cggctgccgg tatcgatcag ggccgtggcg atgatcgaga
     1021 gcgagccgcc ttcctcgatg ttgcgcgccg cgccgaagaa gcgcttgggg cgctgcaggg
     1081 cattggcgtc gacaccgccg gtcagcacct tgcccgagct gggcacgacg gtgttgtagg
     1141 cgcggccgag gcgggtgatc gaatcgagca ggatgaccac atccttcttg tgctcgacca
     1201 ggcgcttggc cttttcgata accatttcag caacttggac gtggcgggtg gccggttcgt
     1261 caaatgtgga ggcaatcacc tcgccgcgca cggagcgctg catgtcggtg acttcttccg
     1321 ggcgctcgtc gatcaggagc acgatcagga agacttccgg atggttgtcg gtgatcgcct
     1381 tggccatgtt ctgcagcagc acggtcttgc cggtgcgcgg cggggcgacg atcagggcgc
     1441 gctggccctt gccctggggg ctgatcagat cgatcacccg ggccgacttg tccttcaccg
     1501 tgggatcgat ggtatcgagc cgcaggcgct cgtccgggta gagcggggtg aggttgtcga
     1561 agttcacccg gtggcgcacg gcatcgggat cgtcgaagtt gacgctgatc agcttgttga
     1621 tcgcgaagta gcgctcgccg tccttggggg cgcgaatctc gccctcaacg gtatcgccgg
     1681 tgcgcaggcc ccatttgcgg acctggttgg gcgagacgta gatatcgtca ggccccgcga
     1741 ggtagtttgc ctcggggctg cgcaggaagc cgaagccgtc ggtcaaaacc tcgatcgtgc
     1801 cgatgccgag aatctcctcg ccatcttcgg ccacttcctt gaggatggcg aacatgaggt
     1861 cctgccggcg catggtgctg gcgccctcga cgccgaattc ctcggccatc tcgaccagtt
     1921 cggccggggt tttcttcttc agttctttca ggtgcattat ggtatccgag tgctgggtgt
     1981 ggccggctcg ggtcatgggg ctgggggaca gcggacctgc gaggctggca tttaggccgc
     2041 actcgcgccg gagacggttg tggagatagg ccgcggcgcc aggaaggtca atcacgcaaa
     2101 gcgaacgcga cggcgttagc cggggcgaaa ttatttccgc cttttttcaa cgacaactgc
     2161 agtaactctg gcggttcccg cgcgatcgcc catgcaaagg gctcaggccc gtgccaagaa
     2221 cggcagcaac gaggggccgg atactttagc gaccatggat ttcgagcatc gcctgaatga
     2281 agcccgagag cagttccacg cggggcaact ccagacggcg ctggaaatcg tgagcgccct
     2341 gttcgaaagc catggcgaac gcggcgatac gctgaacatg gcggcgctgt gccacctcca
     2401 cggtggcgat caggccgggg cgctcgggct gttccgccgg gccgtagccg ccgatccggc
     2461 ctatagcccg gcgtggagca accttggcaa cctcctgcgc acgctgggcg cggaagccga
     2521 gggcgtcgca gccttgcgcc aggcggtggc ggtcgatccc gattgcatcg aggggtggaa
     2581 caaccttggc ctggcggcga tggtcgccgg gcaggcggcg gaggcggagc agctgtttcg
     2641 ccgcgctctc gagatccacc ccggctatgc ggtgagcctc ttcaacctgg gccgggcgct
     2701 gctgatgacc catcgcagcg aggaaagcga ggcggtcctt cgccggttgc tggccgccgc
     2761 gcccgactat ccgttcggcc aggcgtgcct tgcctcggcc ctgatcgacc agggacggac
     2821 agaggaaggg gaagcggcct accggcgtga gcttgaccgc gatcccgaca attccgtggc
     2881 gctcaataac cttggcctgc tcgcccagaa ccagcggcgg ctgcacgagg cgctgggctg
     2941 gctggaacgg gcagccatgc tgccgggggc cgatgcgctg accctgagca attacgccaa
     3001 tgtgctgcgc gaggtggggc ggcccgacga tgctgcccag tggtatcgca ccgccgctca
     3061 gcgcgatccg gaattcacca cggccctgtc gaaatggatg ttgtcggccc gcgcggtctg
     3121 cgactggggc gacctgaccg agctcgaggc gcggattgcc gagcacatcg cgcgcgggat
     3181 cgtcgaagcc ccggccgctt tcgacctgct ggtgcatccg ggcctgaccg acgcgcagca
     3241 ccgggcggtg gccgaggctt atggcgcccg agcctacggc gccgcgctgg cgaggccgcc
     3301 gctggtcccg ccgggacggc cgcttcattc cggcggcggc ccgctgcgga tcggatacgt
     3361 ctcctccgat ttctacgaac atgcgacgat gcggctgctg cgcggcgtgc tggccggaca
     3421 cgatccggcg cgggtgcaat tcctgtgtta ttctaccgga gcgatcatcg gcgacgcgga
     3481 gcgcgaacgg atcgaagcag cgccggcctg cgccggcttc cgcgacctgg cgcggctgcc
     3541 cgacgaagcc gcggcctgcc agatccggga cgatgcgatc gacatcctgg tcgacctcaa
     3601 gggctatacc caggagaacc gccttggcat ccaggccctg cggccggcgc ccgtggtggt
     3661 ttcctggctc ggctatcccg gcaccctggg cgtcgcgcga ttggccgact acatcatcgg
     3721 cgatccggtg gtgacaccgc tgcaggccca ggcggactat tcggaagccc tggcgctgat
     3781 gccccattgc taccagccca acgacgatac catggtcacc gaagcgccgc ccagccgggc
     3841 gtcgcaaggc ctgccggacg acggcccggt gttctgcaac ttcaacgccg cctacaagtt
     3901 ctctcccgcg gctttcgcca gctggatgca cctgctgcgc gccacgccgg gcagcgtgct
     3961 gtggctgctg cgcccgggcg aggcggcccg cgcgcgcctg atcgcggcag ccgcggcgga
     4021 ggggatcgcg gccgaacggc tggtcttcgc cgccccgctg tcgcagcgcg accaccttgc
     4081 ccgcttgcag ctggccgatc tcgcgctcga tacctttccc tacacctcgc ataccaccgg
     4141 cagcgacgcc cttctcgccg gggtgccgct ggtgacccgt ccgggcgata cctttgcctc
     4201 gcgggtggcg gccagcctgt tgacggcggt cggcctgccc gagttgatcg cggacgatga
     4261 tgaggccgcc cggcagctgg tgctggacct ggcccgtgac ggggcgcggc gggccgcctt
     4321 gcgggcgcat ctgctggcag tgcggcgcaa ttgcccgctg ttcgatactt cggggttcac
     4381 ccgggacctg gagcggctgt accacgcgat cggcgaacag ttgcagcgcg gtgagcgccg
     4441 gggatttgcc ctgtcggcgg agccttaggc gctcagttct tcagcgcgat cacttccatg
     4501 caggcgcgat atctttcact caccccatag cgcatcgggg tgaagccgtg ctggcgcagc
     4561 agtgcctcga gccgggtgcg ggtgaagttg tgataatgct cgagctcgcc ccaataagga
     4621 ttggcgccgg cggcatcgag caggtgccag agcgggcttt cggcattggg catcgagagg
     4681 aacaggaccc cgccggaggc cagcatgctg tgggcatggc tgaggacaag ccggggaaaa
     4741 ggggtgtgtt ccagcacgtc agccagcgaa atgacgctga atctcccggg aaactccaag
     4801 gtttccagcg gccgggcatg ggtttcgatg cccagcccgg caagcgctgc ggcattatcg
     4861 gttcgcaggt cgatgccgac cggggtgaaa ccatattcga gcgccgtgag cagcaaggcg
     4921 ccattgccga agcccacatc cagccacggg cccgagctgc gccacggcag gaccttttcg
     4981 atcatccgcg cggcgaccca gcgctgggcc tcgtaatcat ggccgagccg ctggttgtcg
     5041 tgcgtgccgg aaaacagcag ctggcaggct tcatccgtat agaagccact gcgaaactgg
     5101 tgcccgcagg cggcgcaggt gcgccacagc agggtgtcgt tcaaggccgg gttatagagc
     5161 ccatgcccgg cgcaggatgc ggtgcgcagg tcggcaaggt ccgtgcagtc acacaggggg
     5221 caggcggtgt agagctcgag ttcctgcatg atccccccgc acggattgcc gtcaaaacgg
     5281 cttcacgatc accatgacga caatgaacgc cgcggcaatt ccgggcactt cgttgagcag
     5341 gcgcaggcgc ttgccggtca ggcgctcctc gccgcgggcg agggcagcgg cataactggc
     5401 gagcagcagg tgataggcgg tgagggccag gacgaaggcc agcttcacgt gaaaccagcc
     5461 ctgctggaag gcgccgatct gcatggccag agccagcccg aggagccagg tgataatcag
     5521 ggatggccac aggatgatcc tgagcagctt gcgctcacga tcaatccaca ccgcgcgttc
     5581 gggcgaaccg gccggggctt cctgatgata aacgaaatag cgcgggagca tgaacagccc
     5641 ggccatccag aagatcacga agatcacgtg gccggccttg agccagaggt aggtcatcgc
     5701 gagggcctgc tgcatggggt ggattgtagg acggcggcag gggtggggga aggggccgcg
     5761 gatggcaagt ccttcgagac ggcggctcag cccgcggctt cgccacctcc ccgggacgaa
     5821 cggcaatttg agatcaggtc ctgcgccggc tggtcaggtg gaagcggcgg cacttgtcac
     5881 aacggtaggg gcgcgcggca ccggggaagg cgagggcgac cgagcgggcc tcggcgaagg
     5941 aggggtagcg caccttgcgc tggcaaatgc tgagacgggt gcgtttcatg gcgggtcata
     6001 cttctccgcg atcttcttcc cgacgatgct ccctcgcgcg agcctcctcc gcaaccgctt
     6061 gcggcggcgg tccccctccc cgcacgcgag gaggtttcag cggctccagc cgcgcacgac
     6121 tttcaacagc cgctcgacgt gttcgatcgg cgtgaactgg ccgatgccgt ggcccaggtt
     6181 gaacacgtgg ggacggtcgg cgaaggcatc gagcacgcgg cgggcctggc tgtcgagttc
     6241 gtcgccgccg gccagcagca ggagcggatc gaggttgccc tggaccggca ggccggccgg
     6301 cagttcgcgc gcggcccaca gcggatcgat ggtctcgtcc acgccgagcg catcgacccc
     6361 ggtttcgcgg gcataggcga cgagcttttc gcccgcgccc ttggggaagc cgatgatcgg
     6421 cgtatcgggg cagcgcgcct tcagctggcg gacgatttcg gcgttggggg caatgaccca
     6481 gcgttcgaat tcggcggggg cgagggagcc ggcccaggaa tcgaacagct gcaccgcctc
     6541 ggcgccggcg cggacctggc cttcgaggta atcgaccgtg gtggcgacca cggcatcgat
     6601 gatcgcctgg aacgcgccgc gatcacgata ggcataggcc cgggtctcat gctggtcgcg
     6661 gctgccttcg ccggcgacca tgtaggtggc gatggtccac gggctgcccg caaagcccag
     6721 catggtctgc tcaggggtga gcgcggcgcg gacgaggcgc acggtctcat agatcgggga
     6781 aaggcgctca gggacgctct caagggcact gagggcatga tcgacgaggc ggggcgacat
     6841 gcgcgggccc tcgccagcga ggaattcgag atcctggccc atggcgtagg gcacgatcag
     6901 gatgtcggag aacaggatcg cgccgtcgaa gccgaagcgg cgcagcggct ggagagtgat
     6961 ctcggcggcg gcctcgctgt catagaccag cgggaggaag ccacccttcc ttgcgcgcag
     7021 ttcccggtac tcgggcaggt agcgccccgc ctggcgcata aaccacacgg gtcgccggga
     7081 accgttcgtt ccgcgcaggg tatcaaagag gagaccgggc attcgatcgg gtccaatcca
     7141 ataaaataag agtctttaaa tagggattct gatagattct ggccctgtgg aaaccgggga
     7201 ttgggcgccc ttgcccgaat tgtcccggaa agaacagggg ccgggccgat gcgaatcccg
     7261 ggcgctcccg gtcaagcgcg agttatcccg cttgtcccca gcctgtgggg aacgctttcg
     7321 acatgtccac aagtcgcccc ggcgaagacg ggaacagcgc atggaatccc ttgccggaat
     7381 tgtcaccggt gccctcccgt ggtttatgcc gcatcctatc cacagcggac ctgacgcgtg
     7441 cccacaaccc ggcttcacct ccatctcctg tcggattcca ccggtgaaac gctggaaatg
     7501 atcgccaagg ctgcgctcgc gcagttcgat gatgccgatg tgacgcgcca cttctggccg
     7561 atggtccgct cgcagcagca cctcgaccgg atcctcaccg agattgccgc caatccgggc
     7621 ctggtgctgt tcaccctcgt caatgccgag atccgggtgc ggctggaaga ccgctgccgg
     7681 gcgctgggcc tcccggtcgt ccccgcgctc gatgccgtga gcaatgcgct tgaggacatg
     7741 ctcggccagc aggccaaggc ccggccgggg cgccagcaca tgctcgacga ggcctatttc
     7801 gcccgtgtcg acgcgatcca gttcaccatt gcccatgacg acgggctgca gtgggaggac
     7861 tgggaggagg ccgatatcct gctggccggg gtctcgcgca gttccaagac gccgacggcg
     7921 atctacctcg ccaaccgcgg atacaaggtc gccaatatcc cgatcgtgcc ggaaagcccg
     7981 ccgccgccgg tgttgtttca actgggccgg cctctagtgg tgggcctgac caccgcgccg
     8041 gagcggctgg tgcaggtacg gcgcaaccgg ctgctgtcgc tgaaccaggc gccgtcgacc
     8101 cactacgtcg accaggagcg ggtggcgggc gaagttcagt ttgcgcggcg gatgtttgcc
     8161 gataatggct ggccggtgat cgacgtcacc cggcgctcga tcgaggaaac ggcggcggcg
     8221 gtgatccgcc tgcaccagga acaccgcgcc cgcggcgaga cggccggggc cggatcccgg
     8281 gcgatctgag gggcgagtga acgcatgttg atcctggcct cgcaaagcgc ctcccgccgg
     8341 gccatgctgg aagcggccgg ggtgccgttc atggccgtgc ccgccgaggt ggacgaacgc
     8401 gcgctggagg ccagcctcgc aggagcggcg cccgacgtgg tggccaccgc gcttgcccgg
     8461 gcaaaggccg aggccgtggc ggcgcggcat ccggggcggt gggtgctggg gtccgacagc
     8521 ctggtggttt gtgaggggcg gcggttcgac aagccggcca accgcgaggc cgcggccgag
     8581 cacctgcggt tcttttcggg caagacgatg gaacttcaca gcgcggccac gctggtgcgg
     8641 gatgacgcgg tgatcccttg cgcggacaag gccgaactgg ccgtgcgcga gctgtcggag
     8701 gagttcattg ccgattacct tgcccacgaa tggccggcag tggccgggtg cgtcggcgtg
     8761 ttccgcatcg aggggcgcgg ggtgcaactg ttcacgcagc tgcgcggcga tcatttcacc
     8821 atccttggca tgccgctgct gccggtcctc gccgggctgc ggagcgtggg ggtgatggcg
     8881 gcatgaggcc
//
LOCUS       DATNBV010000587         2727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_4469480,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000587 DATNBV010000000
VERSION     DATNBV010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4469480"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VN627_13890"
     CDS             complement(<1..304)
                     /locus_tag="VN627_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04388.1"
                     /translation="MMSSQAAMPRMGEGYSPPASMFAFGQSAADLDIPGDQRQLPVDE
                     LELRHLRSPRDFDAVRALRGHIDLSVHLGVDPQFHEREKKEMSWAWPSHSRCEGKSS"
     gene            541..2034
                     /locus_tag="VN627_13895"
     CDS             541..2034
                     /locus_tag="VN627_13895"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HXD04389.1"
                     /translation="MDQAVDAPQRPLELALAWRHRFARLGPAFYTELPPTPLPSPYLV
                     GLNRALARELGLPGQALAGQDGIEAFTGNLPIAGSQPLASVYSGHQFGVWAGQLGDGR
                     AILLGEIETPAGPQELQLKGSGLTPYSRMGDGRAVLRSSIREFLGSEAMNGLGIPTTR
                     ALCVTGSDAPVRREEIETAAVITRVSPSFVRFGHFEHFSARERYDELAKLADYVIDNF
                     YPDCRATSQFSGNAYAALLEQASERTAAMVAQWQAVGFCHGVMNTDNMSILGLTIDYG
                     PFQFLDAFDPGHICNHSDEQGRYAYNRQPNVAYWNLFCLGQALLPLIGDQELALSALE
                     SYKAVLPRELEARMGAKLGLRDRQPGDRELIEAILKLLAAERVDYTIFWRRLSRHVAG
                     ESAETVRDLFLAREAFDAWLLQYSQRLESAGRTQAADLMLKTNPKYVLRNHLCELAIR
                     QAKLKDYSEVEALLALVQAPYEEHPGHDAKAGFPPDWASQIEISCSS"
     gene            2031..2447
                     /gene="msrB"
                     /locus_tag="VN627_13900"
     CDS             2031..2447
                     /gene="msrB"
                     /locus_tag="VN627_13900"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXD04390.1"
                     /translation="MTYKIEKSDAEWKALLTQKGAEPRAFEVTRRAATERPFTGKYEA
                     VWDDGSYHCMCCGNKLFDSDTKFDAGCGWPSFSQAVPGAIKEITDASLGMVRTETVCA
                     QCGAHLGHVFPDGPTDTGLRYCMNSASLDFKAGEGK"
     gene            2458..>2727
                     /locus_tag="VN627_13905"
     CDS             2458..>2727
                     /locus_tag="VN627_13905"
                     /inference="COORDINATES: protein motif:HMM:NF016189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HXD04391.1"
                     /translation="MKLLLDFFPIILFFVAFKFAGIYVATGVAIAATVIQIVWLRHRN
                     GKVEPMQWVSLAVIVLFGGATIALQNETFIKWKPTVLYWLMAGTLM"
BASE COUNT          499 a          914 c          819 g          495 t
ORIGIN      
        1 ccgacgattt tccctcgcac ctcgaatgcg acggccacgc ccaactcatc tctttttttt
       61 cgcgttcgtg gaactgcggg tccaccccca agtgcacgct caggtcgatg tgcccccgca
      121 acgcccgcac cgcatcgaag tcgcgcgggc tgcgcagatg ccgaagctcc agttcatcca
      181 cgggaagctg gcgctgatcc cctggaatgt ccaggtcggc ggcgctctgc ccgaaggcga
      241 acatgctcgc gggaggcgaa tagccttccc ccatacgtgg cattgctgcc tgactgctca
      301 tcatttgttg ttgctcccct tttggtccgc agcgtcccgg cactggagcc atgccgctga
      361 gccacggcca attatcggca ttcaacgagt aaacgcaaca gtacaggctg cgctggatgt
      421 gtttatgcca ctctgacgga ccggggcgcg cggcccgttc ggtcccgccg gcccacgaac
      481 aatttgttgc caatgccctt gctcccccgc acgggaggcg aagcagggac aatgccaatc
      541 atggatcaag ccgtagatgc gccgcagcgc cccctggagc tggccctcgc ctggcgccac
      601 cggtttgccc ggctgggacc ggcgttctat accgagctgc cgccaacccc gctgccctcc
      661 ccctatcttg tcgggctgaa ccgcgcgctc gctcgcgaac tgggacttcc cggccaggca
      721 ttggccggcc aggacggcat cgaggcattc accggcaact tgccgatcgc gggcagtcaa
      781 ccgctggcca gcgtctacag cggccaccag ttcggcgtct gggccggtca gctgggcgat
      841 ggccgggcca tcctgctagg cgaaatcgag acgcccgccg gcccgcagga attgcagctc
      901 aaaggcagcg gcctcacccc ctattcgcgc atgggggacg gccgtgccgt gctgcggtcc
      961 agcatccgtg aattccttgg gtccgaggcg atgaacggcc ttggcatccc gaccacccgc
     1021 gcgctctgcg tcacgggttc ggacgcgccg gtgcgccgcg aggaaatcga aactgccgcc
     1081 gtgatcaccc gggtgtcgcc cagcttcgta cggttcggcc atttcgaaca cttctcggcg
     1141 cgcgaacggt atgacgaact ggccaagctg gccgattacg tcatcgacaa cttctatccc
     1201 gattgccgcg ccacgagcca attctcgggc aatgcctacg ccgccctgct ggaacaggca
     1261 agcgagcgca cggcggccat ggtcgcgcag tggcaggcgg tggggttttg ccatggcgtc
     1321 atgaacacgg acaacatgag catcctgggc ctgaccatcg actacggccc cttccagttc
     1381 ctcgatgcgt tcgatcccgg ccatatctgc aaccacagcg atgaacaagg acgctacgcc
     1441 tacaaccggc agccgaacgt ggcgtactgg aacttgttct gcctcggcca ggccctgttg
     1501 ccgctgatcg gagaccagga attggcgctg tcggccctcg aatcgtataa agccgtcctg
     1561 ccgcgcgaac tggaggcccg aatgggcgcc aagctcggcc tgcgcgatcg gcaaccgggc
     1621 gaccgcgagc tgatcgaggc catcctgaag ctcctcgccg ccgagcgcgt cgattacacg
     1681 atcttctggc ggcggctgtc gcgccacgtt gcgggcgaga gcgcggaaac cgtccgcgac
     1741 ctgttcctgg cgcgcgaagc gttcgatgcc tggttgctac agtattcgca gcggctggag
     1801 tcggctgggc gcacccaggc agccgatctg atgctcaaga ccaacccgaa atacgtgctg
     1861 cgcaaccatt tgtgcgaact ggcgatccgc caggcgaaac tcaaggacta ttccgaagtc
     1921 gaagccttgc tggccctggt ccaagcccca tatgaagaac accccgggca tgatgccaag
     1981 gccggcttcc cgcccgactg ggcctcacag atcgaaatca gctgctcctc atgacttaca
     2041 agatcgagaa atccgatgcc gagtggaagg cgctgctcac gcaaaaaggc gccgagccgc
     2101 gcgccttcga agtgacccgc cgcgccgcca ccgaaaggcc gtttaccggc aagtacgaag
     2161 ccgtgtggga cgacggcagc tatcactgca tgtgctgcgg caacaagctg ttcgactcgg
     2221 acaccaagtt cgatgcgggc tgcggctggc ccagtttttc gcaggccgtg cccggcgcga
     2281 tcaaggagat caccgatgcc agcctgggca tggtgcgcac cgaaaccgtc tgcgctcaat
     2341 gcggcgccca cctcggccat gtgttccccg acggcccgac cgacaccggc ctgcgctatt
     2401 gcatgaactc agcctccctc gacttcaaag ccggcgaagg caaatgatcg ctatccgatg
     2461 aaactgcttc tcgacttttt tcccatcatc ctgtttttcg tcgcgttcaa gttcgccggc
     2521 atttatgtgg cgacgggcgt ggcaatcgcg gcaaccgtga tccagatcgt ttggctgcgc
     2581 catcgcaacg gcaaggtgga gcccatgcaa tgggtcagcc tggcggtgat cgtgctgttc
     2641 ggcggcgcga cgattgcgtt gcagaacgaa acgttcatca aatggaaacc gacggtgctg
     2701 tactggctca tggccgggac tttgatg
//
LOCUS       DATNBV010000588         2807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3176829,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000588 DATNBV010000000
VERSION     DATNBV010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3176829"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VN627_13910"
     CDS             complement(<1..1352)
                     /locus_tag="VN627_13910"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HXD04392.1"
                     /translation="MNLRATFEHRSGRVIAVLRVVLAFVFLVALAFEPVAAGESVHDG
                     LVLISSYLLFSLVLVAIAWRSWWLDHRLARPVLVADVAIFLGAVFVTEAYDADFTSPF
                     LALFALAVLSSTLRWGSAVAARTGVIVSLLFIALGFVLAGADIPIEVYRFSRRAFYMV
                     ALLLVLVWFGIDRRGAQVPPLVLPRESTDPDMLGDAALAYALRLTGAAHGALAWSDSE
                     EPWTEVHELRTDGSVAHWRAGPEELGAGGEWNARPCLFDQGSGVALVHVGEGRVRGER
                     LIAPIPLASRVGITEGLALPLACSSREGLIVLGGIEGPGPDFLALGLAIAREIETTTQ
                     RLTAARLERESAVIRARSAIARDLHDSVAQSLAGACFRLEALRRNIANGALAAPAEEI
                     AAVRDALRGEQAQVRGLIDTLRDPSGLLAHRPLAADLDRALTEAAAHWHVAASFAIDA
                     P"
     gene            complement(1399..1806)
                     /locus_tag="VN627_13915"
     CDS             complement(1399..1806)
                     /locus_tag="VN627_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXD04393.1"
                     /translation="MDGTHRARALTRLALGLLYGAAGVLHLAFPSPFVAITPRWVPDP
                     PLVIALTGLAELAGAAGLLQPFSPALRRAAGWGLAAYALCVWPANFNHLLIDLARPGH
                     GLGLAYHLPRLAFQPVLIWAALWAGGAPCRRPA"
     gene            1974..2513
                     /locus_tag="VN627_13920"
     CDS             1974..2513
                     /locus_tag="VN627_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HXD04394.1"
                     /translation="MSRARGKNDWGFPRWRGYGSGSAAEPVRMCDRAGCHEKGDCPAP
                     KSPNSPERWYFCQSHAAEYNQGWNYFEGLTKEEAAEREAREQADNSGYKEAAHYGWAG
                     SGDGTRSRDELKALDVLGLDPDADFEAVKKAWRAKAKEVHPDVKPGDAEAADQFRKLQ
                     VSYEVLRAAEERREWKGAS"
     gene            complement(2553..>2807)
                     /locus_tag="VN627_13925"
     CDS             complement(2553..>2807)
                     /locus_tag="VN627_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXD04395.1"
                     /translation="TGVANRDPNLFAAPTEARFDRKGAHYTFGTGENLCLGLHLARLE
                     LRVAVEEFLAAVPTFTLQPGHVMEHALCSIPQPIALPLVW"
BASE COUNT          499 a          903 c         1070 g          335 t
ORIGIN      
        1 ggcgcatcga tggcaaagct ggcagcgacg tgccagtggg ccgcggcttc ggtcagggcg
       61 cggtcgaggt cggcggcgag cgggcgatgg gcgagcaggc cggagggatc gcgcaaggta
      121 tcgatcaggc cgcgcacctg cgcctgttcg ccgcgcaggg catcacgcac ggcggcgatt
      181 tcctcggccg gcgcggcgag ggcaccattg gcgatattgc ggcgcagcgc ctcgagccgg
      241 aagcaggctc cggcgagcga ctgggcgacg gaatcgtgca ggtcgcgcgc aatggcgctg
      301 cgggcgcgga tcacggcgct ttcccgctcc agccgggcgg cggtgaggcg ctgcgtggtg
      361 gtctcgatct cgcgggcgat ggccaggcca agggcaagga aatccgggcc gggtccttcg
      421 atcccgccaa gcacgataag gccctcgcgg ctgctgcagg cgagtgggag ggcgaggcct
      481 tcggtgatac cgacgcggct ggccaaggga attggcgcga tgaggcgctc gccccgcacg
      541 cgtccttcgc cgacgtggac cagcgcgacg ccgctgccct ggtcgaacag gcaggggcgc
      601 gcattccatt cgccgccggc gccgagctct tccgggccgg cccgccagtg cgcgacggag
      661 ccatcggttc gcagttcatg cacttcggtc cacggctcct cgctgtcgct ccaggcgagc
      721 gcgccgtggg cggcgccggt aaggcgcagc gcataggcga gggcggcatc gccgagcatg
      781 tcgggatcgg tggattcgcg tggcaggacc aggggcggca cctgggcgcc gcgccggtca
      841 atcccgaacc agacgagcac gagcagcagg gcgaccatgt agaacgcgcg gcggctgaag
      901 cggtagacct cgatcggtat atcggccccg gccaggacga agccgagcgc gatgaacagc
      961 agcgagacga ttaccccggt gcgcgcggcc acggccgagc cccagcgcag ggtgctcgac
     1021 aggactgcca gggcgaagag cgcgaggaac gggctggtga aatcggcatc gtaggcctcg
     1081 gtcacgaaga cagcgcccag gaaaatcgcg acgtcggcaa ccaggaccgg ccgggccagc
     1141 cgatggtcga gccaccacga gcgccaggcg atcgctacca ggaccagcga gaacagcagg
     1201 taggaactga tcaggacaag gccgtcgtga acgctttcgc cggcggcgac cggctcgaag
     1261 gcgagggcga cgaggaagac gaaggcgagc acgacccgca ggaccgcgat gacgcggcct
     1321 gagcggtgtt cgaatgtcgc gcgcagattc atcgcagccc ccgtcccgcc atgtgaatga
     1381 acggggaaca aaggccggtc aagcgggcct gcgacagggc gctccaccgg cccacagggc
     1441 agcccagatc agcaccggct ggaacgcgag ccggggcagg tgataggcca ggccgaggcc
     1501 gtgccccggc cgggcgagat cgatcagcag gtgattgaag ttggccggcc agacgcacag
     1561 ggcataggcg gcaagacccc atcccgcggc ccggcgcagg gcaggcgaga acggctggag
     1621 caggccagcc gcaccggcca gttcggccag gccggtgagc gcgatgacca gcggcggatc
     1681 cggcacccag cgcggcgtga tcgccacgaa gggcgagggg aaggccaggt gcagcacgcc
     1741 ggccgcgcca tagagcaggc caagggcgag ccgggtgagc gcgcgggcgc ggtgtgtgcc
     1801 atccatgcct gttcttgcct cctgacctgc ctgccgccgt tgcctgccgg ccggcgggat
     1861 agaccagcac tggggttggc gcgagtggta tcctgccccg accgggcgcc gcttgatcgc
     1921 gaccccggtg ccggtgcttg ccggatcgcc ccgcggccca tatctggcca accatgagtc
     1981 gcgcgcgcgg aaagaatgac tggggtttcc cgcgctggcg cggctacggc agcggcagcg
     2041 cggccgagcc tgtgcgcatg tgcgaccgcg ccggatgcca cgaaaagggt gattgcccgg
     2101 cgcccaagag ccccaacagc ccggagcggt ggtatttctg ccagagccac gcggccgagt
     2161 acaaccaggg ctggaactac ttcgaaggcc tgaccaagga agaggccgcc gagcgcgagg
     2221 cgcgggaaca ggcggacaat tccggctaca aggaagcggc gcactatggc tgggccgggt
     2281 cgggtgacgg cacccgcagc cgcgacgagc tgaaggcgct ggacgtgctg gggctcgatc
     2341 cggacgccga tttcgaggcg gtgaagaagg cctggcgggc aaaggcgaag gaagtgcacc
     2401 ccgacgtcaa gccgggggac gcggaagcgg ccgaccagtt ccgcaagctc caggtaagct
     2461 acgaggtcct gcgcgccgcc gaggagcggc gcgagtggaa gggcgcttca taagccggag
     2521 tagtgcacgg ccgcattgct cccgcccagc ggttaccata ccagcggcag ggcaatgggc
     2581 tgtgggatcg agcacagcgc atgctccatg acatggcccg gttgcagcgt gaacgtcggc
     2641 accgccgcga gaaactcctc gaccgcgacg cgcagttcca ggcgggcgag gtgaaggccc
     2701 aggcacaggt tttcgccggt gccgaaggtg tagtgggcgc ccttgcgatc gaaccgggcc
     2761 tccgtcggcg cggcgaacag gttcgggtcg cggttggcta ccccggt
//
LOCUS       DATNBV010000589         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3178607,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000589 DATNBV010000000
VERSION     DATNBV010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3178607"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1387)
                     /locus_tag="VN627_13930"
     CDS             complement(125..1387)
                     /locus_tag="VN627_13930"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HXD04396.1"
                     /translation="METLARQPAPDPAKQPGVLLDIADVRAAAGRIAGQVARTPTLHS
                     KTLSKITGAEIWLKFENLQFTAAYKERGALNALLLLDEERHSRGVIAASAGNHAQGLS
                     YHGTRLGVPVTIVMPKTTPTVKVMQTESVGGNVVLEGETFDEAYEHARAMERQLGLTF
                     VHPFDDPAVAAGQGTVALEMLEDVPELDMLAIPIGGGGLLSGMGTAARGLKPDIGLIG
                     VQAQLFPSMYAKLNHVDLPCGGDTLAEGIAVKVPGEFTSGVLARLVDDIVLVDEAALE
                     TALALLLQIEKTVVEGAGAAGLAAVMENRERFAGRKVGIVLTGGNIDTRLLANVLLRD
                     LARSGRLARLRLTLQDRPGALFKVMEEFNAHQVNIIEIYHQRIFTHLPAKGLITDIEC
                     EARDREQLDSLVHALRAKGYAVSQVELG"
     gene            complement(1442..1804)
                     /locus_tag="VN627_13935"
     CDS             complement(1442..1804)
                     /locus_tag="VN627_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXD04397.1"
                     /translation="MPHTAPIARAARSAFWLAALFAVTMASLPKPPQLGLEQFGDKVQ
                     HIVAFLVLAVLANLGWPRTERVRIIERLSLLGAVIEMIQSIPELHRDCDIRDWIADSL
                     AVIVVAAGFALWSRWRTR"
     gene            1884..>2039
                     /locus_tag="VN627_13940"
     CDS             1884..>2039
                     /locus_tag="VN627_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXD04398.1"
                     /translation="MTQPLKVSFLGLGVMGTAMARHLGNAGHDLTVYNRSPARIEAWK
                     AANPGLRP"
BASE COUNT          344 a          703 c          656 g          336 t
ORIGIN      
        1 acggccgtgc ggaaatcctc tcggccccgg cccacccggc gccttgtcgg ataacaatgc
       61 cccagaccgc gaaagggtta aagaccctta accaaacatt cggggttaat gcgccaaaat
      121 tcgctcaacc caattcgacc tgggacaccg catagccctt ggcgcggagc gcgtggacca
      181 gcgaatcgag ctgttcgcgg tcccgcgcct cgcactcgat gtcggtgatc aggcccttgg
      241 ccggcagatg agtgaatatg cgctggtggt agatctcgat gatgttcacc tgatgcgcgt
      301 tgaattcctc catcaccttg aacagcgcgc cggggcggtc ctgcaaggtc agccggaggc
      361 gggcgagccg gcccgaccgg gcgaggtcgc gcagcagcac gttggcgagg aggcgggtgt
      421 cgatgttgcc gccggtcagc acgatgccga ccttgcgccc ggcaaaccgt tcgcggtttt
      481 ccatcaccgc ggcaagacca gccgcgcccg cgccttcgac cacggtcttc tcgatctgga
      541 gcagcagagc gagcgcggtt tcgagcgccg cttcgtccac cagcacgata tcatcgacca
      601 gacgcgccag cacgccggag gtaaattcgc cggggacctt caccgcgatg ccttccgcca
      661 gcgtgtcccc tccgcacggc aggtccacgt ggttcagctt ggcatacatc gaggggaaca
      721 gctgcgcctg gaccccgatc aggccgatat cgggcttgag gccgcgcgca gcggtgccca
      781 tcccggagag cagcccgccg cccccgatcg ggatcgccag catatcgagt tcgggaacgt
      841 cctcgagcat ctcgagcgcg accgtgccct gtcctgccgc caccgccgga tcgtcgaagg
      901 gatgtacgaa ggtcaggccg agctggcgct ccatcgcacg ggcgtgttcg taggcttcgt
      961 cgaaggtttc cccttcgagc acgacattgc ccccgacgct ctcggtctgc atgaccttca
     1021 ccgtgggcgt ggtcttcggc atcacgattg tcaccggcac gccaagccgc gtcccgtggt
     1081 agctcaaccc ctgggcatgg tttccggccg aagcggcgat gaccccgcgc gaatggcgct
     1141 cctcatcgag caggagcagc gcgttgagcg cgccgcgctc cttgtaggcg gcggtgaact
     1201 ggaggttttc gaatttcagc cagatctcgg cgccggtgat cttgctcagc gtcttgctgt
     1261 gcagcgtcgg cgtgcgcgcc acttgcccgg caatccgtcc ggccgccgcg cgcacatcgg
     1321 cgatatcgag cagcacgccc ggctgcttcg cgggatcggg ggcgggctgg cgggcgagcg
     1381 tttccatggg ccgggcccta acggaaagcg ctctccaagg gaaccggcaa agggcttcgc
     1441 gctatcgcgt gcgccagcgg gaccagagcg cgaaccctgc cgccaccacg atcacggcaa
     1501 ggctgtcggc aatccagtcg cggatgtcgc agtcgcggtg cagctcgggg atcgactgga
     1561 tcatctcgat caccgcgccg agcaacgaaa gccgctcgat tatccgcacc cgctcggtcc
     1621 gcggccagcc gaggttggcc agcaccgcca gcaccaggaa ggcgacgatg tgctggacct
     1681 tgtcgccaaa ctgttcgagc ccaagctgcg gcggcttcgg cagcgaggcc atcgtcaccg
     1741 cgaacagcgc ggcaagccag aacgcggaac gtgcggcgcg ggcgatcggg gcggtgtgcg
     1801 gcattgcacc cgcttctacc gtctcggtcc gctcccgcaa ggcggggctg gcgaagtttt
     1861 cctgacccgt taaacaggcg gccatgaccc agcctctcaa agtttccttc ctcggccttg
     1921 gcgtgatggg cacggccatg gcccgccacc tcggcaacgc cggccacgat ctcaccgtgt
     1981 acaatcgctc accggcccgg atcgaggctt ggaaggcggc caatcccggc ctccggccc
//
LOCUS       DATNBV010000590         2250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U11197 k141_3183438,
            whole genome shotgun sequence.
ACCESSION   DATNBV010000590 DATNBV010000000
VERSION     DATNBV010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945772
            Sequence Read Archive: SRR6185369
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:14:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,757
            Genes (coding)                    :: 2,735
            CDSs (with protein)               :: 2,735
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3183438"
                     /isolate="SMAG_U11197"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VN627_13945"
     CDS             <1..233
                     /locus_tag="VN627_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HXD04399.1"
                     /translation="EAHAEAGRLTAPMPGKVMSFAVKAGDKVTKGQALAVMEAMKMEH
                     TITAPVDGTVAELMFAPGDQVTEGAELLTLAT"
     gene            370..1290
                     /locus_tag="VN627_13950"
     CDS             370..1290
                     /locus_tag="VN627_13950"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HXD04400.1"
                     /translation="MRIAFCCINTKAAPWLEGLRAALPHATVEEWHPGAPQADHAVVW
                     APPQQFVDEQPRLKGIFNIGAGVDALLKLKLPPGTLLVRIDDGGMAAQMAEFVCQAAI
                     RHFRELADYEAEMRQGKWSFRRPRERGDFPLGVMGLGVLGARVAQAAAQFEFPVRGWS
                     RTPRDVPGVRCFAGPAQFDEFLAATRILVCLLPLTPETNGIMNAKNLSRLMPGGYVIN
                     VARGAHLVDEALIALLDSGHLAGAALDVFRSEPLPAEHPFWRHPKITLTPHTSARTLR
                     GESIAQIAGKIRALERGEPIAGVVDVKRGY"
     gene            1382..>2250
                     /locus_tag="VN627_13955"
     CDS             1382..>2250
                     /locus_tag="VN627_13955"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HXD04401.1"
                     /translation="MKLPGKVKLVDVGPRDGLQNEKEAVPADVKIGLVNRLQDAGVRE
                     IEVTSFVSPKWVPQMADNAQVMAGIERRKGVRYSVLTPNMKGFEAAVASRPDEIVVFG
                     AASEAFSQRNINCSIAESIERFAPVVEAAKAKGIYVRGAISVAVGCPYEGEVAPERVE
                     LVARLMKQIGVQHIGVADTIGVGTPVKVQRAMEAALKHYDLEDISGHFHDTYGQALGN
                     TLASLEMGIWQYDASIAGLGGCPYAKGATGNVATEDVVYMLRGMDIDTGIDLDKLVDA
                     GKYISDYLQRKPNS"
BASE COUNT          409 a          677 c          813 g          351 t
ORIGIN      
        1 gtgaagcgca tgctgaagcc ggccgcctga cggcgccgat gccgggcaag gtgatgtcgt
       61 tcgccgtgaa ggccggcgac aaagtcacca agggccaggc cctggcggtc atggaggcga
      121 tgaagatgga gcacacgatc acggcgccgg tcgacggcac tgtggccgag ctgatgttcg
      181 cgccggggga tcaggtgacg gaaggggcgg aattgttgac gctggcgacg tgacagcccc
      241 tcggggttgt catgccggac tcgatccggc atccatgact atgttttctg gcgaggcctg
      301 gattgcgggt caagcctgtc ctgagcctgt cgaagggccc gcaatgacag atcacagcaa
      361 aatagcccca tgagaatcgc cttctgctgc atcaatacca aggccgcgcc ctggctcgag
      421 ggcttgcgcg cggcgctgcc tcacgcgacc gtggaagagt ggcaccccgg cgcgccgcag
      481 gccgaccatg cggtggtgtg ggcaccgccc cagcagtttg tcgatgagca gccgcggctc
      541 aagggcatct tcaacatcgg ggccggcgtc gacgcattgc tgaagttgaa gttgccaccc
      601 gggacactgc tggttcgcat cgacgatggc ggcatggccg cgcaaatggc cgagttcgtc
      661 tgccaggccg cgatccggca tttccgcgaa ctggccgact acgaggcgga aatgaggcag
      721 ggcaagtggt cgttccggcg gccgcgcgag cgcggcgatt tcccgctcgg agtgatgggc
      781 ctgggcgtgc tgggtgcgcg ggtcgcgcag gctgcggcgc aattcgagtt tccggtccgc
      841 ggctggagcc gcacgcccag ggacgtgccc ggcgtgcgct gctttgccgg gccggcgcag
      901 ttcgatgagt ttctggcggc gacgcggatc ctggtttgcc tgttgccgct cacgcccgaa
      961 acgaacggga tcatgaacgc gaagaacctg tcgcggttga tgccgggcgg ctacgtgatc
     1021 aatgtcgcgc gcggcgcgca tctggtggac gaagccctga tcgcgctcct cgacagcggg
     1081 catctggccg gcgcggcgct cgacgtcttt cgcagcgagc cgttgccggc ggagcatccg
     1141 ttctggcggc acccgaagat cacgctgacg ccgcacacct cggcgcgcac cttgcggggt
     1201 gagagcatcg cgcagatcgc cgggaagatt cgcgccctgg agcggggaga gcccatcgcc
     1261 ggtgtggttg acgtgaagcg gggatactaa gccgtcgata ggcgggtgcc cccaccccag
     1321 ccctcccccg gaaggggagg gagcaacacc gctcccggaa ggggagggag aaagacaaga
     1381 gatgaaactt cccggcaaag tcaaactcgt cgacgtcggt ccgcgcgacg ggctgcagaa
     1441 cgaaaaggaa gcggtacctg ccgacgtgaa gatcggcctc gtgaatcgcc tgcaggatgc
     1501 gggcgtgcgg gagatcgagg tcaccagctt cgtgtcgccc aagtgggtgc cgcagatggc
     1561 ggacaacgct caggtgatgg ccgggatcga gcgcaggaag ggcgtgcgct attcggtgct
     1621 cacgcccaac atgaagggct tcgaggcggc ggtggcgagc cggcccgacg agatcgtggt
     1681 gttcggcgcc gccagcgagg cgttcagcca gcgcaacatc aactgctcga ttgccgagag
     1741 catcgagcgc ttcgcaccgg tggtcgaagc ggccaaagcc aaaggcatct acgtgcgggg
     1801 cgcgatctcg gtcgcggtgg gctgcccgta tgagggcgag gtcgcgcccg agcgggttga
     1861 gctggtggcg cgcctgatga agcagatcgg cgtgcagcac atcggtgtcg cggacaccat
     1921 tggcgtcgga acgccggtga aggtgcagcg ggccatggaa gcggcgctca agcactacga
     1981 cctggaagat atttccgggc atttccacga cacctatggc caggcgctgg gcaacaccct
     2041 ggcgagcctg gagatgggca tctggcaata cgacgcatcg attgccggac tgggcggctg
     2101 tccttacgcc aagggcgcga ccggcaacgt cgcgaccgag gacgtggtct acatgctgcg
     2161 cggcatggac atcgacaccg gcatcgacct ggacaagctg gtggacgccg gcaagtacat
     2221 cagcgactac ctgcagcgca agccgaattc
//
